LOCUS       DAOQWR010000001        57654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_576_length_57654_cov_6.004410, whole genome shotgun sequence.
ACCESSION   DAOQWR010000001 DAOQWR010000000
VERSION     DAOQWR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 57654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57654
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_576_length_57654_cov_6.004410"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1220)
                     /locus_tag="PKK36_00005"
     CDS             complement(54..1220)
                     /locus_tag="PKK36_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR92973.1"
                     /translation="MIERRKELETLDGLLKRHRMVGIIGARQVGKSTLADEYLKSVSL
                     PTHRFDLENPDDAARLAEPMLALKELKGLVVIDEVQRSPGLFPVLRVLADRKPIPARF
                     LILGSASPELLRQSSETLAGRIFYHELGGFALDETGVENMDRLWLRGGLPRSYLAASL
                     RESVEWRAGYIRSFLERDLPQLGISTGASTMRRFWTMLAHYHAQTWNASEIARSFGVA
                     HTTIQNYLDQLTSALAVRKLQPWHENISKRQVKAPKVYIADSGILHSLLNQPEKADLE
                     AHPKLGASWEGFAIGQIIRRLGARAEECFYWATHAGAELDLLVVRGRKRMGFEIKRTS
                     APRTTASMQHALNDLKLSHLDVIHAGDRTFPLSEKIRAVPLRCILQEIEPLSAD"
     gene            complement(1359..2183)
                     /locus_tag="PKK36_00010"
     CDS             complement(1359..2183)
                     /locus_tag="PKK36_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR92974.1"
                     /translation="MIILRKIGSIAAVLAGAWLCLRWFEWKSLYYPDRKMDELPSDAG
                     LEYEEVWFVAEDGTRLHGWWIPHPEARGTVIHLHGNAGNISSRIWIAADLNRLKLNVF
                     LFDWRGYGLSRGLPREKGLYRDARAAYEVVRAKYDDAEYPPVILHGQSLGGAVAAQLA
                     LDKPCRGLIIESSFSSVPDMARVMYPALPAVLIRSRFDTASKMPKLKIPVIVAHSRTD
                     DLVPYEQGRKIFEAANEPKTFCELSGGHNEAGWHTGGEYWSAIEKFAAAVFPPENR"
     gene            complement(2180..3304)
                     /locus_tag="PKK36_00015"
     CDS             complement(2180..3304)
                     /locus_tag="PKK36_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNR92975.1"
                     /translation="MKKLLTDPLLLVGSPAAVPDIRYAAGFNAPDAVVCLCIGNEIHL
                     VVSALEYGRARGETCGVILHTPDSLHLESAKRRDPVAWTVALLKRMHVRRVRVPWDFP
                     AGITLKLNAGGIRIEVIEGPAFPERAVKTRTEIECIRESQRAAVKAMKAACARLRAAS
                     VSSKGLLREGRRVLTSEELQLFIDRVLLESGCSADSTIAACGKQSADPHERGSGPLRA
                     AEPVVIDIFPQHRGHGYFGDLTRTVVKGKPSPKVAAMYRAVKAAQEAALGSIRAGVRA
                     CEVHAAVHETFVRHGFQTGLIGGWPQGFIHGTGHGVGLAIHEAPSVSTAENILKAGNV
                     ITVEPGLYYRDTGGVRIEDTVVVTRTGWEYLCPFRKAIAP"
     gene            3717..5069
                     /gene="dnaA"
                     /locus_tag="PKK36_00020"
     CDS             3717..5069
                     /gene="dnaA"
                     /locus_tag="PKK36_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNR92976.1"
                     /translation="MKKSAAAVLWNQACRHLSQVLSKDVYDRWIALIEPGTLADGVLT
                     LEVANNFYLTWLEDNYLSLMQDAVRSVCGEDIAIHLEVNLSVPSQTPPAEPAAKKASP
                     APARKTLADVPLNPKYTFESFVVGSSNQFAHAAAMAVAQSPGKAYNPLFIYGGSGLGK
                     THLMQAIGHSVQQSGRNAVTYLACEVFLNEYIDALQRGAVVAFRKKYRNTDVLLIDDI
                     HFLAGKERMQEEFFHTFNALFDRHKQIVLASDRPANEIPGLEHRLVSRFEWGLVTDLE
                     TPDMETRIAILRKKQELFNISLPPEVLSYIAQRVRSNIRRLEGALIRAASYSSLTGKE
                     LTLDNLERLLRSVLDEEAQDTLSMEAIQRTVAEYYKISHEDMISKRRPAAIAFPRQVA
                     MYLCRTLTDHSLPAIGNAFGRNHATVLHACRLLTEKIQSDPDIRQTVSILNDQLENKG
                     "
     gene            5325..6419
                     /gene="dnaN"
                     /locus_tag="PKK36_00025"
     CDS             5325..6419
                     /gene="dnaN"
                     /locus_tag="PKK36_00025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNR92977.1"
                     /translation="MKLKVEKAAVVEGLQKVLSIVSTRTTLPILSNVLLQAEGGKLHL
                     TSTDLEVSVKTSVDASVSKKGGTTLPARRLASIIRELASHDVEITIEDKDTAVIQAGP
                     SFFKLRGASEDDFPPLPKFEGSRAFTLKQGVFRDMLVKTSYAASTDETRYILNGVLLS
                     FRDEKLTVVATDGRRLALVEQEVEFPKDSEGDLVIPTKTVNELIRTLQDDGSIRIQAS
                     ANQVAFEFGDMLIISKLIEGAFPNFRQVIPSQAEQRVTIEREALMNAVRRVALLASEQ
                     ASTVRLTFANNRLEITAETPDVGEARETLPVKFSAKEITVAFNPEFIVAPLKTLDSDE
                     VFFEVTDALSPGVIKSNVPFLYVLMPMRIN"
     gene            complement(6432..6505)
                     /locus_tag="PKK36_00030"
     tRNA            complement(6432..6505)
                     /locus_tag="PKK36_00030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6471..6473),aa:Gln,seq:ctg)
     gene            6639..10154
                     /locus_tag="PKK36_00035"
     CDS             6639..10154
                     /locus_tag="PKK36_00035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR92978.1"
                     /translation="MSCCFKRRPRGIIPRRGGGCIHTGWLLVLAFHLLVPSASVSARG
                     LEPVKLQLKYRHGFQFAGYYAAKIKGFYEAEGLDVTILEAVRGVYPVDAVLSGEAEYG
                     VMAEELLVERAHGKPLVVMGAIYQHSPYILLALRESGIRTVEHLAGRRVMRSFNRPSP
                     QMDAMLKNADVLPDSVHFVPHDWNIESLISGRADAMDAYLTDEPNLVRLRGLEPVILS
                     PMDYGIDFYGDCFFTTEDEIRLHHRRARGFHCATLRGWAYALEHQEELIDFILTLPGV
                     EERGITREHLQYEAEQSVKLIRPDLIKLGEINPNRWERMAKTYVALGLIPSGFSLEGF
                     IYDPGHEPHWHWLHILLIVLASLLIAAAIFWSWNRQLRRSVAAAIRDRRETDRRAYKL
                     FHEVAQGVVIADTDNGKITYANPSIGRLLGYSKPELLKMESQDLHPAKEWDRVRNAFT
                     AQTEGDHLVASEIPYLRKDGTLMYADVTVSKTRIGGRARNVAFITDITERLEAKRSLQ
                     RSLAVSNRIRKFMVDISGCRTVEGLLEPLLQAAMDICGMDCGGVYIVENDAACLKHTV
                     NLDDELKSLVARMPLANPLMKSAAEAREPVYAHDVSEDMRSMLLSFGIQHAYSIPFFE
                     DGHLFGFLNVGTHCEEPPDSVAIHNLSVLALEAQSVIRRIADEAVRNRMMMAIEQAAE
                     SIVISGPDHLIVYANPAFEKLTGYTLEEVLGVHPHILDAGQHEPGYLEEVEAMVTRGE
                     TWRGQFINKTKDGRIIIEEACVTPVFDPAGAIVNYVTVKRDITRELKLEEQLRHAQKM
                     EAVGKLAGGIAHDFNNLLQTILGYAELIMKSPNADADTRADMEEVYSAAERAAGLTRQ
                     LLTFSRRQVIRPQSVDVNELLTSLVKILRRGIRADIALEVDPGTDLWPVFADPGQMEQ
                     VLINLCFNARDAMPEGGRLLIEARNEEISQEFFRQNPWAREGRYVRLRVTDTGSGIAP
                     ETMERMYEPFFTTKEAGKGTGLGLSTVYGVIEQHNGLIHVESQVGRGSVFDVYFPVAR
                     RDPERPAGAASSPSGNGRETILLAEDDPSVVGYTRRVLERAGYTVLTASTGVEAVELF
                     SRSTGRVNLVLMDVVMPGLTGVAARDEMLKIRPDIPILFISGYSHGAIRAERIAKEKI
                     EIIQKPFRASDLLARIREILDRRPA"
     gene            10362..12881
                     /locus_tag="PKK36_00040"
     CDS             10362..12881
                     /locus_tag="PKK36_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR92979.1"
                     /translation="MTITAKKMLKDNPILTDAQLRAEVEKCEFCEEKPCKAACPCDCS
                     PADFIMAVKLGTPADFKRAAAEIMSKNPFGGICGMVCPDTLCMGACVHKKMDCAVNIP
                     AVQATIIAKARELKSFPEFEKVKFNGRTVAVVGAGPAGLAAAATLAQKGYKVDIFEQN
                     EKSGGMCRLIPEHRLYREMLEKDVDFCLGMGGSRIKMKTGKAVSDPKTLLKKGYKAVI
                     VAAGLWEPVILNIPNKDKAIGGIEFLQKPKSFGLRGKKVAVIGGGATALDCAVAAKLS
                     GASSVEMIALENVGEMPLTPREKQDLVEHGIDFNGRMRVTEICANGDKVTGLKTIKIA
                     LPAGEKFSVSALKDIKGTEQTRADIQIVIMAIGARSTIPKVDSRGVFYAGDIANGPTF
                     VVTASAAGKNCAMVVDAALNQQKAPVIKDPKKSTVLVEGYNMVPVSLETDFFGRKLIN
                     PFLLSASPASDGYEQMKKAYEAGWAGGIMKTAFDNLPIHIPAAYMTCFNDTTWGNCDN
                     VSDHPIDRVCTEVKKLVREYPDRLTGASTGGNVSGNDESDKKSWQSNTRKLEKAGAMV
                     IEYSLSCPQGGEGAEGDIVSQNAALTAKIIGWVLEVGDPSVPKLFKLTSAVTDIKSIL
                     KAVKPVFAAYPKAKAGVTLANTFPSLAFKPSPAKKVWEDGVVSGISGEGITPVSYLCL
                     ASAGGMGVTISGNAGPMNYKAAADFLALGTNTVQFCTVVEKHGYLIIDELCSGLSHLM
                     QARKIKSVKQLVGIAQPEPIRGFMDLDGTKQISTCSTDLCVQCGNCTRCPYLAISMNA
                     DNYPETDASRCIGCSLCVLQCPAKALSLRDRTAKEKKALHA"
     gene            12992..13077
                     /locus_tag="PKK36_00045"
     tRNA            12992..13077
                     /locus_tag="PKK36_00045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13025..13027,aa:Leu,seq:gag)
     gene            complement(13084..13914)
                     /locus_tag="PKK36_00050"
     CDS             complement(13084..13914)
                     /locus_tag="PKK36_00050"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNR92980.1"
                     /translation="MKRSTNRIWITQVVPAAGGRRLKVTFRDGEASWAARAPDCWEMK
                     LPGFEPVTCSPYSKVRRGALSSETGAVYLKKFLLRGWRDAFKGMFRTSRAGRAWRGDL
                     LMEKSGFSAPHVRCLIRPAGFFRSESMLITDEVSNAVSVKKRLMDPQYGLAAGTSRRF
                     AFLRAVAEAAARLHAAGICHGDLRTNNILSRETGEGWEFIWLDNERNRKYRTLPRRKR
                     NHNLMQFNTDREPSLADRVCFWRAYARAAGLTGDDRIGTARWIRNRTRERWIRHGFMK
                     "
     gene            13978..15579
                     /gene="murJ"
                     /locus_tag="PKK36_00055"
     CDS             13978..15579
                     /gene="murJ"
                     /locus_tag="PKK36_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNR92981.1"
                     /translation="MSKTHFIKSTAIVSGFTALSRVLGLVRDMLMAGVFGTSLYMSAF
                     VVAFTIPNLFRRLFGEGALSASFIPVFIEVRHKEGEGPAWIMVRKVVTLLTLTLLAIV
                     IAAMLVVTAASLWMDLGEKAALVMPLLRIMLPYTVFICLAALSMAILNSYHHYAVPAF
                     TPCLLNFVWIVAVLFICPRFTGDPMGQIGAVAWAVIAAGVLQLGVQIPQLVKYGYRPG
                     LSFDWKDRRMIRILTLMGPAALGLAVTQFNVVIDRLLAIWIGPWAPAALFFSERLIYL
                     PLGVFAVALSTVLLPTFSGHMAVTDTVRMKSTLNRALRNLLFVMIPAAAGLLILSGPI
                     VEMIFEWGNFTRQSTRLTALALQFYAPGLVVFSLAKVFVPAYYAMQDTRTPVRIGLYA
                     VLLNLILNVTFVLTWPLYWKHAGLAFATVLAEGFYALALAVVLHRRIGSPGWRSIGSS
                     VSLCAACTVMMGLTVVYVHSALTLHPGILPLDGKLMQVANVLISIAAGMAVYFGASAA
                     LKSSELADVAAALRRRKPQTSSTNT"
     gene            complement(15555..16652)
                     /gene="hemW"
                     /locus_tag="PKK36_00060"
     CDS             complement(15555..16652)
                     /gene="hemW"
                     /locus_tag="PKK36_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNR92982.1"
                     /translation="MNYGLYIHVPCCLRKCSYCDFHSQAMTDGETRAFADALESELDS
                     LPEDFSPATIFIGGGTPTALPEDVLSRLLGAVRGRVNTGRVEEWSCEANPGTLTEGKI
                     QVLLNGGVNRVSLGIQTFDEKLLKFLNRIQTGEEALKSFRALRTAGFQNIGADLIYGL
                     PGGSTRQVENDLNRLLELGPEHISCYCLSIEPGTALARMSVSEADDEEALAQYRLILK
                     TLGAAGYEHYEISNFARTGRECRHNLLYWEGGEYIGCGPSAHSHWNGTRRANEKDTQD
                     YILHGPRRAFEERLAPEARARETLVMSLRKIRGVSQSLFRKQTGFDYYALCGPAIEKL
                     IADGLLKQDGDFLQLTESALFVSNQVFVELV"
     gene            16757..18052
                     /locus_tag="PKK36_00065"
     CDS             16757..18052
                     /locus_tag="PKK36_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR92983.1"
                     /translation="MKKNYVLDTNVLLHDPNAINSFKDCNVVIPLTVLEEIDNFKREL
                     TERGRNARQLSRQLDELRHRGRLSEGVALDNGGGLRVLFHGDLEAFAESRKNDDIILN
                     AALDLKKSATEPTILISKDINMRLKADAIGLDAEDYESGQVKSPEFHCGYIELEVDSQ
                     SFSRFEHEHKLPMEGEELNPNLYIVLEEESNPGHQALGRYDGKTKSIVPLIALPSQMH
                     PIHPRNTEQRFAMDALMNDDIRLVTLTGKAGTGKTLLAVAAGFFKTVDEGTYTKLLVS
                     RPTLPMGRDLGYLPGDLEEKLNPWMQPIQDALDLVRTGRKAGQKSDRDFTMFGDKISV
                     EPLTYIRGRSIPGQFIIIDETQNLTPLEVKTIITRVGHDTKIILTGDIYQIDNPYVDI
                     LSNGLNAVAERFRPHAVAAHVMLKTGVRSELAELAANIL"
     gene            18062..19591
                     /locus_tag="PKK36_00070"
     CDS             18062..19591
                     /locus_tag="PKK36_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR92984.1"
                     /translation="MKKIISIALLAGLLLCPASRARDAALVRQQYDMVKSRLDDGGDL
                     MIVANVDGLVEAGVQKLVEMASMAASTQPSAPGLPAALEKLPAFLKANGFYAVTAVGF
                     SAVPKDNGLNTIKAFVGRDAAAAALPLWRGLFGLQGIEMITPAYLPADTEIVRTFAAD
                     PEQLWKLSREAVTRLGTPEGAMAFEQSLAMSTMMLGVPLDTLISSLGGEGFLSMQLSK
                     TASAELPLGGAGKTMTVAEPSVLLGFTVKDETLATLIKTRFCGEGLPAALTEKDGAEI
                     ITMSMKLPSPIPMQITLAVKSNVFLIGSNPGVINRALAAAESGEGLLASEKFKTAFGS
                     LPMKNNGMMYFSPECAEFFKTIQQAAVMNDSGADADMNAAIMKLFGSDEPQQSAMVIL
                     NTRNGIQLQGNTTFSGRDVLAGAVFAPVGIVAGMMLPAVSSARAKAQTVACMNNLRQI
                     QAAKDQWAIEKGMERGAPVSPSDIEPYLREIPTCPAGGSYILNAVGEEPTCDHPGHGM
                     K"
     gene            19599..20267
                     /locus_tag="PKK36_00075"
     CDS             19599..20267
                     /locus_tag="PKK36_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNR92985.1"
                     /translation="MLTGGYFIFFIPAMILSMIASAMTKGTFRKYARVAASSGMTGGE
                     AARRLLDSQGIDSVSVNHIGGFLTDHYHPLKKELNLSDGVYGSSSLAAIGVACHEAGH
                     ALQHAHGYGPLMFRSAMVPVTQFSSRISYLVLILGLAMNFRPLVDIGIILFSVAVLFS
                     LVTLPVEWNASSRAKRLMVSSGIVTAAEEDDAGKVLNAAFLTYVAAAVSSIMTLLYYI
                     SRRR"
     gene            complement(20271..20684)
                     /locus_tag="PKK36_00080"
     CDS             complement(20271..20684)
                     /locus_tag="PKK36_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR92986.1"
                     /translation="MKRLILALFAMIVAVSAIADSYITSPTTGERRYKWDGTYLMKPN
                     TGERLYKWDGTCIMKPNTGERLYKWDGEYLMRPNTGERLYKWDGTYIMKPNTGERLFK
                     FDGTYLMKPNTGERLAKVDGIFSIPVLIAYATGLL"
     gene            20838..21170
                     /locus_tag="PKK36_00085"
     CDS             20838..21170
                     /locus_tag="PKK36_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNR92987.1"
                     /translation="MDFRTYQERARSTAVYPGMGEGNWIYPALGLAGETGEICEKIKK
                     CIRDDGGKMKPERLADIEKELGDVLWYIAALGSELGLDMETIAERNLAKLAHRKKNGK
                     IHGDGDHR"
     gene            21173..21763
                     /locus_tag="PKK36_00090"
     CDS             21173..21763
                     /locus_tag="PKK36_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HNR92988.1"
                     /translation="MKIDLHVHASERSACSVSGEEDMIRAAARRGLDALVFMDHHCLV
                     PVSRLQELNREFAPFRIFGGVEITLSEEEDILVLGVRDPRLEQRNWTMPDLHSFVRDR
                     GGWMALAHPFRYRDNVNFDMEKYPPDAIEVRSSNIAAGNLKRIEALLGLTGLPAICTS
                     DAHIANRAGLYYVELDQTPADETELARLLRARKFCA"
     gene            21760..22263
                     /locus_tag="PKK36_00095"
     CDS             21760..22263
                     /locus_tag="PKK36_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR92989.1"
                     /translation="MTGDEIFPVVDENGTEIGRARRSECHGNPALIHPVVHVIVRSRR
                     GEIFLQRRNIRRDVQPGKWDTSVGGHMHPGEEAAGAAQREMQEELGVRPPRLQFAYRY
                     LWRSETESELVHTFLAEHEGPFILQAEEIDEGRFWTEGEIRAAVGSGKLTPNFEAEFE
                     RLVKHES"
     gene            22253..23185
                     /locus_tag="PKK36_00100"
     CDS             22253..23185
                     /locus_tag="PKK36_00100"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HNR92990.1"
                     /translation="MNLDRELGVALEAVKAGCLLSRAVQQGITSVAKEDRSPVTIADL
                     GTQCVISMRLMNHLPGETLIGEEDTKIFEGREQLKSQVLELVRRFEPDTDEDQMMGAI
                     RFGSQPAVPERFWVLDPIDGTKGFLRGDQYAIALGLVVDGRVVLGVLGCPNYGERMFY
                     AVTGRGAFAVHLNGGDKKQINVDDVADPSRAVFCESVESAHAAHGDHAKISEILGMTR
                     APFRMDSQCKYAAVAQGDASIYLRLPSRKDYQECIWDHAAGSILVEEAGGRVSDCDGK
                     ALDFTAGRRLVNNRGIIATNGRIHDAVLAAIAQL"
     gene            complement(23187..25598)
                     /locus_tag="PKK36_00105"
     CDS             complement(23187..25598)
                     /locus_tag="PKK36_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNR92991.1"
                     /translation="MKSSPPSEYLPGPRRPLVGLAVAVMAGCLAGLRGPPCFLLPAVT
                     AFVLTVAAMVFRKHPAAGAAVYAALVLTAAAHTSLLHHSPSRRDVRSMMRHSREYLQL
                     MGVVTDDPESEPGFGRFSTSWSFTLKLEGVRRREQWQAARGRVFVRISLPEADPPLRY
                     GDRLIVEGLLKNLASPRAWLPPVTMEGEDGRTRILSGGNGSRLKGWCYASRARFSLVL
                     SRGLENYPDIRGLLEALLLGYRSALPDNLRDVFALTGTLHIFAISGLHVGVMAILIIA
                     VLKMLGISRQYWILLLGPALILYVMGTGLKASAARACIMALLYWSGPLFKRRPDGGST
                     LAAAAILILAADPRQIASPGFILSFSVVAGIILFYPVFRRLYTLPLRKPRWIPGPRPR
                     LERWGRFLAEKAADLAAVSTAAWLVSAPTTAYYFNLCSPIGLLGNIFIVPGSFLVVLT
                     GCLSLVTGSLFPVCAEIFNHANRVFVDLLLWIVRLMSSIPHGHSHVQSPSPLLMLLWY
                     ITLSALVFMRMGFRKIFFCGILIASLAAFSLAWYRQDVAIDVLDVGEGNAVFMNLPRD
                     GDILIDTGPHYFANRVIRHLQAEGVDRLETVVLTHPDADHTGGAEAVMDRFEVGEVWV
                     CPFKGQSERYERMLEKVRARGIRVRRLERGLRGTAAGDVVWEVFSPEGTKNFRIANEA
                     SLVMRFGRKAAAILFMGGAGISEERAMLQAGIQPAAGILVLGDHGSEKTTSDEWLTAV
                     SPRLAVISVGRNNRRGDPSPTVVQRLDSRNIERWRTDLQGSLRITLPDDPQAAPWIVG
                     PVPNK"
     gene            complement(25606..26412)
                     /gene="thiD"
                     /locus_tag="PKK36_00110"
     CDS             complement(25606..26412)
                     /gene="thiD"
                     /locus_tag="PKK36_00110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNR92992.1"
                     /translation="MSQNNTAVAALTIACSDSGGGAGLQADLMSFSAMGVFGTCAVTA
                     ITARSPTEVRTVVPLLPELVLDQIRMVCESFPIAAAKTGLLYSADIIRAVVDADISQG
                     IPILVVDPIMVSRTGVRLLQADAIEILCNELLHEARVVTPNLHEAEILCGHTISSLED
                     LQRAAREIGEKYDVACVVKGGILPGEEVYDVLYDEGEEYVFPVPRIHVAEKHGAGCAF
                     SGALTALLARGELICDAVRTAQSFVTDALNRAYRIGPHTPLNLRDARPGL"
     gene            complement(26487..26906)
                     /locus_tag="PKK36_00115"
     CDS             complement(26487..26906)
                     /locus_tag="PKK36_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNR92993.1"
                     /translation="MTVSPVSREKAELLRKRMQALGIRDDDLTEKFVRGAGRGGQKIN
                     KTSSCVYLIHGPTGIEVKCQRERSQALNRHLARRELCDRLEEQREKRKSARRQAAEKI
                     RRQKRRRSRRQKQRMLEDKRHQGEKKRMRAGVTGGGE"
     gene            complement(26931..28871)
                     /locus_tag="PKK36_00120"
     CDS             complement(26931..28871)
                     /locus_tag="PKK36_00120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNR92994.1"
                     /translation="MSLIDYSSELNEEQCRAVLAPDGPVLVIAAAGTGKTRTLTYRVA
                     HLVERGIDARRILLLTFTNRAAREMLERAAKLVGAAVGGLWGGTFHHMANRILRRHAD
                     RLGYGLDYTILDQDDSRKLVKAISEELDYAGKHFPKPDVLLSLFGYAASTGESLRLVA
                     EERFKYHDEINPEQVIAVAEAYEERKRRLNGMDFDDMLLNVLRLFRQEPGICASYQEQ
                     FQHILVDEYQDTNYLQSEWVDLLASKNRNLFVVGDDFQSIYSWRGANFRNILSFPERY
                     QGAKVFKLETNYRSVPEILNVANACIAGNPEQFQKTLRSVRENYKVPSVAVLRDGSAQ
                     ARYIIEQIARLKRSGYKTSEMAVLYRSHFHAMELQMELTRERIPYVITSGARFFEQAH
                     IKDVCAIVRLLRNPVDELSFLRILEFLPRVGRRSAAKIWESLGGRFDASEQALAAVRA
                     KLPAPARESWQAIETVITEITRDPEGAGAEAYMEAFVDGFYDEYAVETFDNYNRRQED
                     IQELMNYASQFATPDDFLSEMALLSNLDAEDDHPADDGKGVIRLSTIHQAKGLEWKVI
                     FILWLADGMFPSAKSLEVPGGVSEERRLFYVATTRAKDELFLCVPEVRRGRDGSTQFC
                     ITSRFVEEIPEKLVSRPYVGFI"
     gene            complement(28917..30251)
                     /locus_tag="PKK36_00125"
     CDS             complement(28917..30251)
                     /locus_tag="PKK36_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNR92995.1"
                     /translation="MNMSEFKQKCRRLKQFAFRDVWDMEVSSLPALRAIPVRVYRIGH
                     LVIKGFIDDELSIRASALTYTALMSLVPMLAIVFSLLKGFGAGQAGMDRFMEWQTTMP
                     QQFQDFIVKTMDIVQNTNFAALGWAGFGVLLLATITVLGSIEASFNRIWGIAISRNLI
                     RRMANYISILVVVPILIGVAGTVMATLNSERVIQNLGSIGPVYTGFLKLSPVFSAWLA
                     FSFLYVFLPNTRVKAWPAFWGSLIGALLWLGWQKTYISLQVGVARYNAIYGTFASIPI
                     FLAWVYISWVIVLLGAEIAFAVQHHDTYHIERESERASLRARATLGMALITAAAERLE
                     KGDGCLVIKDFAAEHLIPVRLLYDVVKLYTEAGWLSPVADREDAYVLLKAPERLLLKN
                     VINVIMDAGVSAEYLGLDRLKGKVPAVMARYGRALEAGLENKSVLDLIDGLV"
     gene            complement(30263..30799)
                     /locus_tag="PKK36_00130"
     CDS             complement(30263..30799)
                     /locus_tag="PKK36_00130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR92996.1"
                     /translation="MRETTIGSRTVFKGRLLTVDVLDVELENGVRSTREVVRHRGAAA
                     VLARLPDGRFVLVRQFRKPVEREVLEIVAGLLEEREAPEACARREVREETGHEVEELV
                     RLGTVCPSVGCMDEHIQLFYARLADGPAATRLDHDEHVETVVLSAGEITDMIRRGAVE
                     DAKTLAAWLLYEKMFPNP"
     gene            30937..31950
                     /gene="trpD"
                     /locus_tag="PKK36_00135"
     CDS             30937..31950
                     /gene="trpD"
                     /locus_tag="PKK36_00135"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNR92997.1"
                     /translation="MIKETIAKLVDGKELTRQESYETLKEIMSGNATPAQIGSFITAL
                     RIRGESADVIAGGVQAMREKLIAIATPEGNVVDIVGTGGDQQHTFNISTTAAFVVAGA
                     GVMVAKHGNRRVSSSCGAADVLTELGVNIEIGPPQMEKCIREAGIAFLFAPALHPAMK
                     YASAPRSEVGIRSIFNILGPLSNPTGARRGVLGVYTRDLVKLFSGALAQLGSVHMFVA
                     HGLDGLDEITTTGKTAMGEVRGGDIRLFELDPRDYGIPLTDSASLRGGDSAANALLVK
                     EVLSGKTGPKRDIVLLNAAVTIMAGGGAETIEAGLTAAAKSIDSWAAMEKLEKLVALT
                     NEK"
     gene            32230..32565
                     /locus_tag="PKK36_00140"
     CDS             32230..32565
                     /locus_tag="PKK36_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR92998.1"
                     /translation="MKKSGFTLVEIMIVVAIIGLLAAIAIPNFVKARQSSQQKSCMNN
                     LRQIDGAKDQWAIDEGKTSSDTPGKADLEPYLKSWPICPAAGAYTINAVGTEASCSIH
                     GTVLSPTTL"
     gene            32680..33006
                     /locus_tag="PKK36_00145"
     CDS             32680..33006
                     /locus_tag="PKK36_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR92999.1"
                     /translation="MRARGFTLVEILIVVGIVGLLAVLAIPSFMKSREKSQAKTCMNN
                     LRQIYYAKEHFASQNGLNAGDPVTENDVAVFLNHGLPVCPAGGAYSVNPVGSDPSCTV
                     AGHICP"
     gene            33071..33781
                     /locus_tag="PKK36_00150"
     CDS             33071..33781
                     /locus_tag="PKK36_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNR93000.1"
                     /translation="MRKDIFMKRIALTAVTVLLCLSASAYDTDAVKFKDGPHAGFSFG
                     GDVRSEDFAWGWQAAYEHSDWLSLEASITAQNDELEQSINDVAIPQPFGLELDVYALA
                     FSARAGIRPWSRLHLYALAGAGYYIMKTDAEEARVIIAGAEPSPSGVRLSGLTVDVEN
                     SFGLHAGAGLEIRLSERWEIFAEFRQVFLEADADLKITEEWPSGVPNNPRMMQTRMVD
                     DKLPYDHSLVRAGVSYRF"
     gene            complement(33791..34948)
                     /locus_tag="PKK36_00155"
     CDS             complement(33791..34948)
                     /locus_tag="PKK36_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93001.1"
                     /translation="MATEKNLSSTMRIEISPAALKDLVQQRDRSTSFVKTSTGIKVRT
                     KKSTQIMDSKYQHLLQSIYDGVLIADLSGKITDVNIRALDFLGHDRDELLQMNVVDIL
                     AGAEKTLLSALRDNLQNERFALIQAYCIRRDGTFFPAEIAVNLLKFADPLMCFFLRDI
                     SLRKEAEEKMITSFNAIQNAGTGIAIADVDGRLTYVNPSFLRQWGIHDQNDVLHTDLV
                     RLWQNDTHAGEMVRAVLNDLVSWRGEMVAVRADGSSFESEVSATPNFDSEGDLVGMVL
                     SVEDITNRKRAEEGMRLAEQHRAMLASLGAACHHLGQPATVLLANLEMLKQDKFRESE
                     VFSEIIGLSCEAAERIAEILHKMKEVEEFRTTRYISTTGDSDLPTNRILDL"
     gene            complement(35077..35832)
                     /locus_tag="PKK36_00160"
     CDS             complement(35077..35832)
                     /locus_tag="PKK36_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93002.1"
                     /translation="MKKTVTAAALLALAGNLHADTLAERMLAAYDAVETVSCEVRRDT
                     ESAQGKSRMLSRVFCQKPDLIHIENVMPSLGTGRRRIVSDGKALYIHEQDEPRGYSRP
                     VSELDADKLIELRRLPGTAMDHLLRLKGVEEQPLPGTPEFPVRAGYQTARNFVVLCAD
                     GESRLVQVEFYRTSAMDERIGRYAYSSFSEPLPGVFFAGLIRGEFSDMGLQLTETVRI
                     SSLAVNEPVSPMLFVPGAFFENVEFVPNTGAGS"
     gene            36042..37124
                     /locus_tag="PKK36_00165"
     CDS             36042..37124
                     /locus_tag="PKK36_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93003.1"
                     /translation="MIGKKTGGRMAENASNPVIAAADLKKVFRDFWRRPKVRAVDGIS
                     FEVSRGEVFGLLGPNGSGKSTTIKMLLGLLFPTSGRLRVLGESPRHVKTRSRVGYLPE
                     HSYIYPHLTSEETLDFYGRLFDLSRRERRRRMEQLLEMIGLQHARHRMVGEFSKGMAR
                     RIGLAQALINDPDVVVLDEPTSGLDPIGCRQVKDLILTLARRGKTVLMSSHLLADVED
                     VCDRIAILYNGKIQAMGPVRELLEEKGRRRITLPELSPEAMARVLELLRKEFGEEPEL
                     EHPRRDLEQFFLDVVEKARRGTQASPPKKEKLAGYLEGEDLLQKLAAPSTTETKREEP
                     PPSAPPPAARTDARLSHLLQNGPDNE"
     gene            37117..38472
                     /locus_tag="PKK36_00170"
     CDS             37117..38472
                     /locus_tag="PKK36_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93004.1"
                     /translation="MNRVLSIAAVTLRTAVRSRIVVFMMLLLVFILTGLPSAIRGDGT
                     LTGYVQVLLSYTLGFAGFILSVVAVWTGCTAVSAEIRTHQMDLVVTKPVPAVQIWFGK
                     WLGILALSLALLLFTFAAAYGGLRWAMIHRSASEVDIQKLREQVLVAREKIAPRDEGL
                     EEQAENLYRLRQAEGAVPEGVAPSTARRIIADDLRLQANSVPPGYGRRWIFDFGSFVK
                     DRPLFLRYTFTASRAEIEGLWTAGESGSRDQAVRRVTEASGGVRTWRIPSAAARDGSL
                     TIEFGNIHEEPATVIFPPENPPVLLLYHDSFEMNFLRVFCIAFIRIAVLAAIGLTAGT
                     LFSEPVALFFSAWMVLLLNIGRYIRAMAATDVFFPVHDGPARIPGLLDRLLHLFFAAL
                     DGLVRPLQSPDVLGAMAAGEWVSWGLVAAEFLVKVLIFCGLLAALSSFVLNRREVGLP
                     A"
     gene            38469..39791
                     /locus_tag="PKK36_00175"
     CDS             38469..39791
                     /locus_tag="PKK36_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93005.1"
                     /translation="MNRLVNILLLLAAVGLILLAGARHEELRRLKMEYEFTLVDPVEN
                     ASPLVAFTTVALGGFRGLAADALWLRATRLQDEGRYFELVQLSNWITQLEPGFGPVWA
                     FHAWNLAYNISVKYSEAEDRWRWVQSGIQLLRDKGLVYNPASAELYRELAWIFHHKVS
                     GVTDQAHWFYKQQWADEMERALGGATPDFEALETRADALDRYRLEPAFMQQVENTYGP
                     LDWRLPQAHAIYWAAKGRPFARGFYEVALDRIIFDSLAEAFRRGKLFRDDERGVFINS
                     PNLDLLPRVQAAYAKALADYPEEDSIRRGHENFLREAIMIIYSHNRVRQARELFADLK
                     ERYGSHIEADTLEEFIPAATADFLDKRAPREALPLIEGALYQSALWSALGEADLAAGH
                     RQLAKLYWDHFMESAVSEEWIERMALPPLEELAAKAEKQAADYLQQMP"
     gene            39917..39991
                     /locus_tag="PKK36_00180"
     tRNA            39917..39991
                     /locus_tag="PKK36_00180"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39949..39951,aa:Cys,seq:gca)
     gene            40014..40436
                     /gene="gcvH"
                     /locus_tag="PKK36_00185"
     CDS             40014..40436
                     /gene="gcvH"
                     /locus_tag="PKK36_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNR93006.1"
                     /translation="MKVPEHLLYTRTHEWISIEDGLATVGITGFAAQQLDDVAYVELP
                     AAGDRFMPGDEAAVVESVKAASDIYAPVAGAVEEVNGELHDHPELVSQDPYGEGWLFK
                     IRLAVEDDHDGLLSEEEYRKLIPNAGQETFDDGSGPVF"
     gene            40411..41109
                     /gene="lipB"
                     /locus_tag="PKK36_00190"
     CDS             40411..41109
                     /gene="lipB"
                     /locus_tag="PKK36_00190"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNR93007.1"
                     /translation="MTAVALYFDELQPFGPVSDLQSRLVQARHTGAIPDTVLFLEHKP
                     VVTLGRRGRDRFLKLPSEEYARRGIELMTSPRGGDVTWHGPGQLVMYPVIKLGDREAD
                     THGYLRNMEEIAIRTAADFDVKAFRREGKSGAWTEQGKLAAIGFRVSRWVTSHGMSFN
                     VSPDLSGFDTIVPCGLEGEAVASLKSILGERCPSLSAARDALKTRFEEVCERPLEVFA
                     SCAPPAELAGFMNF"
     gene            complement(41096..42349)
                     /locus_tag="PKK36_00195"
     CDS             complement(41096..42349)
                     /locus_tag="PKK36_00195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNR93008.1"
                     /translation="MQSADGNIQIQAAAIVEAQLEKGLFPDRLLDSVREQHGLLLELV
                     YGAIRWRASLEWMIRELAPRPPALRLRALLLTGLYRLLYMNSAKPYFVVSECVDAARA
                     AGAEKAARFVNAVLRNALRRRRELLSGVARQKPEIRLSCPEDLFRRWTDQWGREDAVR
                     LCKWNNRRARTVLCIPAAMDSIRAALEKRKIAAVPHPFDPQHFLVLPPGCPVRDLPGY
                     HDGNFTVQDPATTLAVSLLNPQPGEKILDACSAPGGKALMIAGAMRRTGSLTAMDLYP
                     ERLDRLKENMNRTGADFIRVLQGDAKMSPRDDAFDGILLDVPCSNTGVIRRRPDAKWR
                     FSEKRLRELCRTQRMILDHQAELVKPGGRIVYSTCSLEPEENTLMVNHWLAAHPDFRL
                     DESRLLFPPDSGTDGAFAARLVRNS"
     gene            42590..44695
                     /locus_tag="PKK36_00200"
     CDS             42590..44695
                     /locus_tag="PKK36_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNR93009.1"
                     /translation="MSPKKNESKSEAREMLTDIYGENVFSLKVMRDYLSDAAFKSLSG
                     TIRSGATIDPRIADEVADAMKIWALERGATHYTHWFQPLTGTTAEKHDSFIVPDGEGG
                     AIAKFSGEELIQGEPDASSFPSGGLRATFEARGYTAWDPTSPAFIKEDGQSRTLCIPT
                     VFCGYHGEALDKKTPLLRSMKALNTQICRLAKLFGVKSDGCRVITTLGSEQEYFLIDK
                     ELHEARLDLLQCGRTLFGRSPAKHQQLEDHYFGAIKSRVVAFMTDLDRELWRLGVPAR
                     TRHNEVCPGQFEIAPMFEELNLAVDHNMLTMEIIRRVAEEHGFACLLHEKPFAGVNGS
                     GKHNNWSICGPDGKNWLSPGDDPHENAKFLTVICALIKAVDTHADLLRAAVASPGNDH
                     RLGANEAPPAIISIFLGEQLTDIIDQIEKGGANRSHKGGMIELGVDSIPPLPRGNTDR
                     NRTSPFAFTGNKFEFRAVGSEQNPSAANVVLNTIMAEALDEICTKLEKAGAEEFNAAL
                     QSILSDIIRKHKRVLFNGDGYTDEWVAEAERRGLPNLKKTPEALASYRTEKAKALFSK
                     YRVLSERELASRYEVYIEAYHRVVKIEARCAVTMARTMIAPAAIRAQTELAGNIKSVK
                     EAGGSAAGAQSLLEEICRHTEMMLSAIKSLESRIAEDDVSGMISATLALREPVDELEG
                     LLPDDAWPLPSYAEMLFMM"
     gene            44709..49277
                     /gene="gltB"
                     /locus_tag="PKK36_00205"
     CDS             44709..49277
                     /gene="gltB"
                     /locus_tag="PKK36_00205"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNR93010.1"
                     /translation="MQTIRKERDDVPVRQCRNCFPAPQGLYDPQREHDACGVGFLVNI
                     QGEKSHRIIEQGITVLKNLLHRGATGADPETGDGAGLLFQIPDAFFRKACGALRMDLP
                     PAGEYGVGMLFLPRDPDRRALCRAAVERAAEDEGLRFLGWRDVPVDSSVLGRKALEEM
                     PFVAQCFVSGASGETLERKLCVMRKQAQGKAGAVHAPGDLFYIPSLSSRTIVYKGLML
                     PKQIAAFYPDLNDEALVSALVIVHQRYSTNTFPSWALAQPFRYLAHNGEINTLQGNRN
                     WMASRENNLRSDVLGDDIRKVLPVIEPGGSDSAAADNVLELLCRAGRSLDHSVAMMIP
                     QAWGTKYPIGPDLRGFFEYHSGLMEPWDGPAAVAFSDGRFIGAVLDRNGLRPARYTLT
                     RDGFMVFASEAGVLDIPPEDVKEKGSLRPGEMIMADVQTGRLMKNIEIKQGLARRQPY
                     RRWVEENRIDIHGFFSAVNPVTADEKNLNFREKLFGYTREDLTVILGPMAATGHEPVG
                     SMGSDQPLAVLSEKPQLLYWYFKQIFAQVTNPPIDPYREELVMSLMTFLGNTDDILSE
                     SPQHARLVKLQHPILSNEDLQRIRELPHEDFRSVTLKMDFFSRGSGRDLEDALDSLCR
                     QAETAVRSGARIVILSDRDLAGDRAPIPALLATAAVNRHLIGHGLRTRTGLLVETGEA
                     REVMHLALLLGYGASAVNLYLAYEIIADLAVKKQLPVNLPVTRATKNYFVALQKGLLK
                     IMSKMGISTLRSYRNAQVFEAVGLSSELISRYFTGTPSRVEGIGLDGIAREARARYDF
                     AQDRPLYGDVLTSGGQYAFRKDGERHLWTADTITLLQQAARENDREKYRRYAELINNQ
                     TQRQSTLRGLLGFRAQNPVPLEEVEPAGEIMKRFVTGAMSFGSISREAHETLAVAMNR
                     IGGKSNSGEGGEDAARYRPLPNGDSRCSAIKQIASGRFGVTAEYLVNAKELQIKIAQG
                     AKPGEGGQLPGHKVNAEIARVRHSTPGVTLISPPPHHDIYSIEDIAQLIYDLHCANRE
                     ARVSVKLVSETGVGTVAAGVAKAKADMILISGYDGGTGASPLSSIKHAGVSWELGLSE
                     VQQTLVLNNLRSRVRLQVDGQLKTGRDVVIGALLGAEEFGFATAPLVVCGCLMMRDCH
                     LNTCPVGVATQDPRLRKCFRGKPEHVVNFMRFVAEEVREYMALLGVRRLDDLVGRTDL
                     LQMNEAVDFWKARGIDLSRVLYQAPGPEQDRRCTQKQEHGVEDALDLEIMDRIRPAIE
                     KAERVDLTLPIRNSRRTVGAMISSEIARRRGGQGLPDDTITLRFSGSAGQSFGAFCSR
                     GMTLILEGEANDYAGKGLSGAKIIIRPPAGSAFPPSESVIAGNVLLYGATSGELYLNG
                     RAGERFCIRNSGAIAVAEGAGDHGCEYMTGGRVVILGPVGVNFGAGMSGGIAYVYDET
                     GTFDENCNLDMIDLESVSSAEDEQELRGMIQKHFDCTGSSRAAEILRNWDSARALFVK
                     VFPMEYKKALGRLSREDAETERAEVVND"
     gene            49270..50679
                     /locus_tag="PKK36_00210"
     CDS             49270..50679
                     /locus_tag="PKK36_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNR93011.1"
                     /translation="MTKRFGFLDRRRDPGYRPAVVRVQDFEPVEQTLTPEQMREQAGR
                     CMECGTPFCHGHGCPLANVIPECNELVYQGRWADALELLLITNPFPEFTGRICPAPCE
                     TSCIAGLHGEPVTIREMERAVMDYGYENGLLSRKPPVRKNQRVAVIGSGPAGLAAAEM
                     LNREGYRVTVYDDAEKPGGILRYGIPGFKLDKALVDRRTGLMREAGIVFECGVRVGED
                     VAFRYLRDRFDAVCLCFGARKPRDLEVPGRELAGVHFAMDYLVQQNLREDGHAIDPGA
                     EIAAYGKKVVVIGGGDTGSDCLGTALRQGAASVLQIEIMPQPPADRAPENPWPEWPRI
                     VRATSSHKEGGTRRWCVNTKAFLGNRGRVCGMRCVEVEWSGGKCSEKEGSEFEIPADL
                     VLLAMGFTGPAENRLAASIGLELDARGNIRTNADRQTTVDGVFAAGDAVSGQSLVVRA
                     IADGMTAARGIMRYLEARP"
     gene            complement(50834..52075)
                     /locus_tag="PKK36_00215"
     CDS             complement(50834..52075)
                     /locus_tag="PKK36_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93012.1"
                     /translation="MSKRSKFFWGSVLTVGLLTGAAGTLAFRAHRVWNVAKDMRAQLR
                     YLTAGDETIGEVPASDAFDADYLKSIFGEDPELFHQLRTVVAKGLEETPVLNLGEVSA
                     MMVTHRKGDDGQPRDVVVHVMGGFPPDARKIGFHRDGYMKKMVDSHLWDAGNIALGVL
                     GRDMVLFAQEETIGPHQELIAAIFRGDIMPLVESLDDPIYFTLVFPDPQHIVPPQLKR
                     HIQAVIMKGYLGQKDGKTDVIFLTPSARSARYAFSILQDLKTAAELALQVKWNGVKTP
                     DRWRAEPTVQCWWAYEIVQNSKASTFEREAGIIRLSSSYGRVMNNAVLKIIERFGRDA
                     RQMQLAYAPQTEPVGDDEKAYWSLTHHWGPDWPVGSDGMIVAGAPATEPAPAEPAAVE
                     PAPAEPADAEVPADEGPAAAQ"
     gene            complement(52164..53156)
                     /locus_tag="PKK36_00220"
     CDS             complement(52164..53156)
                     /locus_tag="PKK36_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNR93013.1"
                     /translation="MQKHRILILFPLLFICMTFSVSGWGGAIHAVINEAAAQNVPEEM
                     SAWRAYAPLLAEHGADPDFWKRDDPAEPVRHFIDLEHYGTPPFEIPRNLSPLKNGARR
                     EGEAPWAIMDTMERLTEAMREKDWYQAARIAAAIGHYVGDTHQPLHCTVNYDGYQTGN
                     AGIHLRWEVQMPRRIGSAATVTSPEPAVYITNLWNCVNGWIMEAWPLCANILQADNEA
                     RWASRNNVESQYYFFQLWEKSQDVFLRQISRAAADLSSVWYTAWVDAGRPEIPPPPRQ
                     ISSASIHVGRAARSPKEENRKGAIAVLAVIGAAGLIIIISSVFKQRRQNTPPAK"
     gene            53222..53542
                     /gene="cutA"
                     /locus_tag="PKK36_00225"
     CDS             53222..53542
                     /gene="cutA"
                     /locus_tag="PKK36_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNR93014.1"
                     /translation="MKHYFVYITCSDAEQAKRMALALVEEGLAACANIIPGMRSVYRW
                     KGAVEQAEEAVLIAKTTEDMAGPLTDRVRDLHSYECPCIEMLPVADGNPDFLRWIEDS
                     VNKS"
     gene            complement(53543..55003)
                     /locus_tag="PKK36_00230"
     CDS             complement(53543..55003)
                     /locus_tag="PKK36_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNR93015.1"
                     /translation="MANKIDELLRLMVAHEASDLHLTTSLKPYLRIHGSMQRIEDHPE
                     LTQKDTETYLLPLVPEDNMKVFAEKWDTDFAYELEGVGRFRVNIFMDHNGLGGVMRII
                     PSVIPNADKLGLPQSVRNFCYLSKGLVVVTGPTGSGKSTTLAAMIDMINNDRREHIIT
                     IEDPIEFVHPTKRCLINQREVHKHTRSFSNALRAALREDPDVVLVGEMRDLETIEIAI
                     ETAETGHLVFGTLHTTTAASTVDRIIDKFPPDRQNQIRTMLADSLKGVVAQTLCKRRQ
                     GGRVAVMEVLVINTAVASNIREGKTHHIPSAMQTGKSEGMQVFNDELIRLIRDGVITP
                     KEAYLKAIDKIEVLKKITEAGFTLDLQGTDSEGVQHEALLQEAHARLQENPSDVEALN
                     TMAWVMSTCVDSHIRDGAQALKIAKQAFKLSKGRDVRVMQTLAAACAENGNFKEAIGV
                     AQSAAKQAAKEGQSELAGEIQKNITLYEAGRPLREA"
     gene            complement(55023..55526)
                     /locus_tag="PKK36_00235"
     CDS             complement(55023..55526)
                     /locus_tag="PKK36_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR93016.1"
                     /translation="MGQFLPLDDLQTYKQTLQHIPHLKDFRGKYLDELFRASELCDFA
                     AGETIVEEGEFGHQIFFLLLGEVAVTKKGVPVASIRGYGEIFGEDPILNQERRAATVK
                     AVTPCYCLSVHTQRLEMLNDQEQRSCFAVIHHICAQVLSRRLKETTEELARVENELQV
                     LRSQMVK"
     gene            complement(55614..56018)
                     /locus_tag="PKK36_00240"
     CDS             complement(55614..56018)
                     /locus_tag="PKK36_00240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNR93017.1"
                     /translation="MPAASPFEVEILVQAEDIDELGHVNNTVYLRWVQDAAVAHWTKI
                     APAEAREHLRWVVIRHEIDYRAPARAGETVIARTWVGSAKGLQFERRTEILRAADRRL
                     LARARTLWCPIDAETDRPRRVSPEVRSLFSAG"
     gene            56216..56659
                     /locus_tag="PKK36_00245"
     CDS             56216..56659
                     /locus_tag="PKK36_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93018.1"
                     /translation="MEQLSLLSKISLYVMLAFMGLYTLLLLGWQVMILRGRSFKNPDG
                     SSDDWHEQKNFYGIAFADVFVACPVNVIGLVLVFIAPRWGFYLLAQAAFWWVWANVMT
                     TATSIRFYNPRRSLGTWLLGYPFGILLGLAYIAWTIVHFDVIYPP"
     gene            57074..>57654
                     /locus_tag="PKK36_00250"
     CDS             57074..>57654
                     /locus_tag="PKK36_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNR93019.1"
                     /translation="MNTASSRKPRPMKECLIKPVEPLILTIRGQRVILDADLAQIYGV
                     ATKRLNEQVKRNADRFPEDFMFQLTKEEKDKVVANCDHLTRLKYSPQLPMTFTEHGAM
                     MAAMVLNSPKAVAMSVFVVRAFIQMRERLVANADILKRLAEIDKTLLEHNDALALIWE
                     QIQPLLAPPPEPPPKQIGFHVKEKRAAYTIRKTG"
BASE COUNT        11474 a        17010 c        17673 g        11497 t
ORIGIN      
        1 cggcgaattt ttcaatcgcg gaccagtatt cgcccccggt atgccatccg gcctcagtcg
       61 gcggacagcg gttcgatttc ctgcagaatg cacctgaggg gaaccgcgcg gattttctcc
      121 gacagcggga aggtgcggtc gccggcatgg ataacatcga gatgactcaa tttcagatca
      181 ttcagggcgt gctgcatgga ggcggtcgtc cttggcgcgg aagtgcgctt gatttcaaag
      241 cccattctct ttcggccgcg caccaccagc agatccagtt ccgctccggc gtgagtcgcc
      301 cagtaaaaac attcttccgc acgggcgccg aggcggcgga tgatctggcc aatggcgaag
      361 ccctcccagg aggctccaag ttttggatga gcttcaaggt cggctttttc gggctgatta
      421 agcagcgagt ggagaatgcc gctgtcggca atgtacacct tcggcgcctt gacctgccgc
      481 ttggatatgt tttcgtgcca cggctgaagc ttccggacag ccagcgccga ggtgagctga
      541 tcaagatagt tttgtatcgt ggtgtgcgcg acgccaaaag agcgggctat ttccgaagca
      601 ttccatgttt gcgcatgata atgcgccagc atggtccaga acctccgcat cgttgatgcg
      661 cctgtgctga tgcccagttg aggaagatcc ctttccaaaa agctgcgaat atatcctgca
      721 cgccattcga cactctcccg cagcgaggcc gccaggtagg agcggggcaa tccgccgcgc
      781 agccaaagac ggtccatgtt ttcgactccc gtctcgtcga gcgcaaagcc tcccagctca
      841 tgataaaaaa tccgtccggc cagagtttcc gaactctggc gaagcagttc gggcgatgcg
      901 gagcccagaa taaggaaccg tgcgggaatc ggcttgcggt cggccagaac ccgcagcacc
      961 ggaaagagtc cgggactgcg ctgcacctca tcaatgacca ccagcccctt aagttctttc
     1021 agggccagca tcggttcagc caggcgggcc gcatcgtccg gattctcaag gtcaaaccgg
     1081 tgggttggca aagagacgct tttcagatat tcatccgcca gcgtggactt gccgacctgc
     1141 cgcgcaccga tgattccgac catgcgatgc cttttcagca ggccgtcaag cgtttcaagc
     1201 tctttgcgtc tttcaatcat gcctcctatt taccttgaaa tttgaaatcg gtcaactcat
     1261 ttttcaagat tattcataaa ctctgaatgg gcattttttg acgttttttg gccatttttc
     1321 gcgttttttg agcgtttttg gccgattttt tcgtgttttc agcggttttc gggcggaaaa
     1381 acggcggcgg cgaatttttc aatcgcggac caatattcgc ctccggtatg ccatccggcc
     1441 tcgttgtggc ctccgctgag ctcgcagaat gttttgggct cgtttgcggc ttcaaagatt
     1501 ttccggccct gctcataggg caccaggtca tcggtccggc tgtgcgccac gatgaccgga
     1561 attttcagtt taggcatttt ggaagccgtg tcgaatctgg accggatcag gaccgcgggc
     1621 agggcgggat acatcacgcg cgccatgtcg ggcacgctgg agaaggagct ctcgatgatg
     1681 agaccgcggc agggcttgtc gagcgcgagc tgcgcggcca ccgcgccgcc cagcgactgc
     1741 ccgtgcagga tcacaggagg atattccgcg tcgtcatatt ttgcgcggac cacttcgtag
     1801 gccgcgcgcg cgtcgcggta cagccccttt tcgcggggaa gcccccggct gagaccgtat
     1861 ccgcgccagt caaacagaaa cacattcagc ttcagccggt tcaggtccgc cgcgatccaa
     1921 atgcggctgc tgatgttccc ggcgttgccg tggagatgga tcaccgttcc gcgcgcttcg
     1981 gggtggggga tccaccagcc gtgaagcctt gtgccgtcct cggccacgaa ccatacctcc
     2041 tcgtattcga gaccggcatc cgacggcagt tcatccattt tccggtccgg ataatagagg
     2101 cttttccatt caaaccagcg caggcagagc catgcgcccg ccagcaccgc cgctatggat
     2161 ccgatttttc tgaggataat catggagcga tcgcctttct gaacgggcag agatattccc
     2221 agccggtccg ggtcaccacc acggtgtcct cgatgcgcac gccgcccgtg tcgcggtaat
     2281 agagaccggg ctcgacggtg atgacgtttc cggccttcag gatattttcc gccgttgaaa
     2341 cggacggcgc ctcgtgaatg gcgaggccga cgccgtgacc ggtgccgtgg atgaatccct
     2401 gcggccagcc tccgatgagg ccggtctgaa atccgtgacg gacgaacgtt tcgtggacag
     2461 ccgcatggac ttcgcacgcg cgcactccgg cgcggatgct tccaagcgcg gcctcctgcg
     2521 cggctttcac cgcgcgatac atcgcggcaa ctttcgggga gggttttccc ttcaccaccg
     2581 tgcgggtcag gtcgccgaaa tatccatgcc ccctgtgctg cggaaagatg tcaatcacca
     2641 ccggctcggc ggcgcgcagc ggtcccgatc cgcgctcatg aggatcggct gactgctttc
     2701 cgcaggcggc gatcgtggag tcggcggagc agcctgattc gagcagaacc cggtcaatga
     2761 agagctggag ctcttcggag gtcagcaccc ggcggccttc gcggagcagg cctttgctgc
     2821 tgacggaagc ggcgcggagc cgcgcgcagg cggccttcat ggccttcacg gccgcgcgct
     2881 gcgattcgcg gatgcattca atttcggtgc gggtcttcac ggcgcgctcc ggaaacgcgg
     2941 ggccctcgat cacttcaatc cggatgcccc cggcgttgag cttcagcgta atgcccgccg
     3001 gaaaatccca cggcacgcgc acgcggcgca catgcatcct cttgagcagc gcaacggtcc
     3061 acgccaccgg atcccttcgc ttcgcggatt ccagatgcag gctgtcgggc gtgtgcagaa
     3121 tcactccgca tgtttcgccg cgggcgcggc cgtattcaag cgcggagaca accagatgaa
     3181 tttcatttcc gatgcagaga cacaccaccg catccggcgc gttgaatccg gcggcatagc
     3241 ggatgtccgg aaccgcggcc ggagagccga ccagcaggag aggatctgtc aataactttt
     3301 tcacagcggc atcctaaaat aaaaaacaaa ataatacagc aaaaacgggg acggcctttc
     3361 taaccgcttc gacgcaaaag atttcaacag gggccgccag tatccccacc atatcttgtg
     3421 ggtgttgaaa attttgcacc cagaaatggt gggccgcgtt ttttttcggg ttatccacag
     3481 tttttccctt gagcggtttt ttttattggc ctagttttgc gtcacacaga tgaaagagac
     3541 gtcgcggaac acgccacgtc cgacggagca tgtgctgttt gaaagccgga tcgcagccgg
     3601 ccggaaacgg aggtctgaac agcggaatga tacgcagcat tccgccggaa ggaaaaccgg
     3661 ataacagctc cgggcttcgc gcggacaggc gccgccggat aaatttttgg gtgaacatga
     3721 aaaagtcagc agcagcagtt ttgtggaacc aggcgtgcag gcatctcagt caggtccttt
     3781 cgaaggatgt ctatgaccgg tggatcgcgc tgatcgagcc gggcactctc gccgacggtg
     3841 tcctcacact ggaagtcgca aacaatttct atctcacgtg gctcgaagac aactatcttt
     3901 ccctgatgca ggacgccgtg cggtcggtct gcggagaaga catcgccatc cacctcgagg
     3961 tgaacctgag cgttccctcc cagaccccgc ccgccgagcc ggcggcgaaa aaagcttcac
     4021 ccgcgcccgc cagaaagaca ctcgcagacg tcccgctgaa tccgaaatac acgttcgaga
     4081 gtttcgtggt cggttcttcc aaccagtttg cgcacgcggc cgcgatggct gtcgcgcagt
     4141 ccccgggcaa ggcctacaat ccgctcttca tctacggcgg atccgggctg ggaaagaccc
     4201 atctgatgca ggccatcggc cattccgtcc agcagagcgg gcggaacgcg gtgacgtatc
     4261 tcgcctgcga agtttttctg aacgagtaca ttgacgcgct tcagcgcgga gccgtggtgg
     4321 ccttccggaa gaaataccgg aacactgacg tgctgctgat tgacgacatt catttcctcg
     4381 ccgggaagga gcgcatgcag gaggagttct tccacacctt caacgcgctc ttcgaccggc
     4441 acaagcagat tgtgctcgcc agcgaccgtc cggcgaacga aattcccggc ctcgaacacc
     4501 ggctggtgtc gcgctttgaa tggggactgg tcaccgatct ggaaaccccg gacatggaaa
     4561 cgcggattgc gatcctgcgc aagaagcagg agctcttcaa catatcgctc ccgccggaag
     4621 tgctttccta tattgcgcag cgcgtgcgct ccaacatccg ccggctcgag ggggcgctga
     4681 tccgcgccgc ctcctacagt tcgctcaccg gaaaggagct cacgctcgac aacctggagc
     4741 ggctgctgcg ctccgtgctg gacgaggagg cccaggacac gctcagcatg gaagccatcc
     4801 agcggaccgt ggccgaatac tacaagatca gccatgagga catgatcagc aagcgccggc
     4861 cggccgcgat cgccttcccg cgccaggtgg ccatgtacct ctgccggacg ctcacggacc
     4921 actcgctgcc ggcgatcgga aacgcctttg gacgaaatca tgcgaccgtg ctgcacgcct
     4981 gccggctcct cacggagaag attcagagcg atccggacat ccggcagacc gtttcaatcc
     5041 tgaacgacca gcttgaaaac aagggatgag gagggctgtg gacgggctga tgataatttg
     5101 ttgaaaaagt gcgtgtcatt cacaggccgc gcttttatca tcagcggtca aaaaatggcg
     5161 ccggttatga acccggtttt caacaggaaa aggatggatg aaaaggccgc tgaatggcgg
     5221 tgaaaagatg ttgttgtatt tgttcacaga ccataataat aggaatattt aaatatatat
     5281 atatcctatt atcagtacgg agtgaataac cggagaaaat gggcatgaaa ctcaaagttg
     5341 aaaaagcggc cgtggtcgag ggtcttcaga aggttctttc tattgtcagc acccgaacca
     5401 ctctcccgat tctttccaat gtgctgctcc aggccgaagg cgggaagctt catctgacct
     5461 ccaccgatct tgaagtcagc gtcaagactt ccgtggatgc ctctgtttcc aaaaagggag
     5521 gcacgacgct tccggcccgc cggcttgcaa gcatcatccg cgaactggcc agccatgatg
     5581 tcgagatcac catcgaggac aaggacacgg ccgtcattca ggccggcccc tccttcttca
     5641 agctccgcgg cgcgagcgag gatgattttc cccctctgcc gaagttcgaa ggaagccgcg
     5701 ccttcaccct gaagcagggc gtgttcaggg acatgctggt caagacttcc tatgcggctt
     5761 cgaccgacga gacgcgctac atcctcaacg gtgtgctgct cagcttccgc gacgagaagc
     5821 tgaccgtggt ggccaccgac ggccgccggc tggcgctggt cgagcaggaa gtggagttcc
     5881 ctaaggattc cgaaggcgat ctcgtcatcc ccaccaagac tgtcaacgag ctgattcgca
     5941 cgcttcagga cgacggcagc atcaggattc aggcgtcggc caaccaggtg gcctttgaat
     6001 tcggcgatat gctgatcatc tccaagctga tcgaaggcgc gtttccgaat ttccggcagg
     6061 tcattccgtc gcaggcggaa cagcgggtga ccatcgagcg cgaggcgctg atgaatgcag
     6121 tccgccgcgt tgccctcctg gcgagcgagc aggccagcac cgtcaggctg acctttgcaa
     6181 acaaccggct cgaaatcacg gcggagacgc cggatgtcgg cgaggcccgc gaaacgcttc
     6241 cggtcaaatt ttccgccaag gagatcacgg tggccttcaa tcccgaattc atcgtcgccc
     6301 cgctcaagac gcttgacagc gacgaggtct tctttgaggt gaccgatgcg ctgagtccgg
     6361 gagtgatcaa gagcaatgtc ccgttcctct acgtgctgat gccgatgcgg atcaactgat
     6421 ccgcgggagg atggttgccc gactaggatt tgaacctaga ctgagcggtc cagagccgct
     6481 tgtgctacca ttacaccatc gggcagaaaa actcacagga atctacggaa ttctccgcgt
     6541 aagtcaaaaa ttttctttga ttcactgaaa aaaccggtgc tccgggaggc ccttgcgcct
     6601 tgacatttcc gcggaaacgg gagcagaatc cgtcctctat gtcatgctgt ttcaagagga
     6661 gaccgcgcgg aattatcccc cgaagaggag gagggtgcat tcataccggc tggcttctgg
     6721 ttcttgcctt tcatcttctg gttccttccg caagtgtttc tgcccgcggg ctggagcccg
     6781 tgaagctcca gcttaaatat cgccacggat tccagtttgc cgggtattat gctgcgaaaa
     6841 tcaaggggtt ttatgaggcg gaaggcctcg atgtgacaat tctggaggcc gttcggggcg
     6901 tttatcctgt ggatgcggtg ctctccggag aggccgagta cggcgtgatg gctgaagagc
     6961 ttctcgtgga acgcgcccac ggcaagccgc tggtggtgat gggggcaatt tatcagcact
     7021 ccccctatat tctactggcc ctcagggaaa gcggcatccg aacggtggag catcttgccg
     7081 gccgccgggt gatgaggtcg ttcaaccgac cgtctcccca gatggatgcc atgctgaaaa
     7141 atgcagatgt gcttcccgat tccgttcatt ttgttcccca tgactggaac atcgagtcgc
     7201 tcatcagcgg tcgcgcggat gccatggacg cctatctgac cgatgagccg aacctggtcc
     7261 gcctccgcgg cctcgagcct gtgattctgt ctccgatgga ttacggcatt gatttctacg
     7321 gcgactgctt tttcacgacc gaagacgaga tccgcctcca ccaccgccgc gcaagaggtt
     7381 ttcattgcgc gacgctcagg ggctgggcct atgcgctgga gcatcaggag gagctgattg
     7441 atttcatcct cactcttccc ggggtggagg aacgggggat cacccgcgag caccttcaat
     7501 acgaggcgga gcagtcggtc aaactgattc gcccggacct gatcaagctg ggtgaaatca
     7561 atccaaaccg ctgggagcgg atggcgaaga cctatgtcgc gctggggctg attccttccg
     7621 gcttcagcct ggaggggttc atttatgatc ccggacacga accccactgg cactggctgc
     7681 atattctgct gatcgtgctg gccagcctgc tgattgccgc ggcgatcttc tggtcatgga
     7741 accgtcagct ccgccggtcg gttgcggcgg ccatacggga tcgccgcgaa accgaccgca
     7801 gagcctacaa gctgtttcac gaggtggcgc agggcgtggt gattgccgac acggacaacg
     7861 ggaagatcac ttatgccaat ccctccatcg gccggctgct cggctattca aaacccgaac
     7921 tgctcaaaat ggaatcacag gaccttcatc ccgcgaagga gtgggaccgt gtgcggaacg
     7981 cgttcaccgc ccagaccgag ggcgaccacc tcgtcgcttc cgaaattccg tatctccgga
     8041 aagatggaac gctcatgtat gcggatgtca cggtgagcaa aacccgcatt ggcggccgcg
     8101 cccgcaacgt ggccttcatt accgacatta ccgagcgcct ggaggcgaaa agaagtctgc
     8161 agcgatccct cgctgtcagc aaccgcatcc ggaagttcat ggtggacatc agcggatgcc
     8221 gcacggtgga gggcctgctg gaaccgctgc ttcaggccgc catggacatc tgcggcatgg
     8281 attgcggcgg ggtttatatt gtcgaaaatg acgcggcctg cctgaagcat acggtgaatc
     8341 ttgacgatga actcaaaagc cttgtggcgc ggatgcccct tgccaacccg ctcatgaaat
     8401 cggccgctga ggcccgggag cctgtctatg cccatgatgt ttcagaagac atgcgcagca
     8461 tgttgctctc attcgggatt cagcatgcct attccattcc cttttttgag gacggtcatc
     8521 ttttcggctt tctgaatgtc ggcacccact gcgaggagcc gccggattcc gtggcaatcc
     8581 acaacctgag cgtgctggcc cttgaggccc agtcggtcat ccgccgcatt gcggacgagg
     8641 cggtgcggaa ccggatgatg atggccatcg agcaggccgc cgaaagcatt gtcatttccg
     8701 gtcctgacca cctgattgtc tatgccaatc ccgcattcga aaagctgacc ggatacacac
     8761 tggaagaagt gctcggtgtg cacccccaca ttctcgatgc cggtcagcat gagcccggct
     8821 atcttgaaga agtcgaggcc atggttacaa ggggagagac ctggcgcgga cagtttatca
     8881 ataaaacaaa agacggccga atcattatcg aggaggcctg cgtgaccccg gtttttgatc
     8941 cggccggagc gattgtcaat tatgtcaccg tcaagcgcga catcacccgc gaactgaaac
     9001 tggaagaaca gctccgccat gcccagaaaa tggaagccgt cggaaaactg gccggcggca
     9061 tcgcgcatga tttcaacaac ctgctccaga cgattctcgg ctatgcggag ctgatcatga
     9121 aaagtccgaa cgccgatgcc gacacccggg cagacatgga ggaggtttac agcgcggcgg
     9181 aacgcgccgc cggcctgacc cgtcagctgc tcactttcag ccgccggcag gtgattcgcc
     9241 cgcagagcgt agacgtcaac gaactgctga cgagcctggt caaaatcctc cgccgcggaa
     9301 tccgtgccga tattgcgctc gaagtggatc ccggaacgga tctgtggccg gttttcgccg
     9361 accccggcca gatggagcag gtgctgatca acctttgttt caatgcgcgc gacgccatgc
     9421 ccgaaggagg ccggctgctg atcgaggccc ggaacgagga aatctctcag gaattcttcc
     9481 gccagaatcc gtgggcccgc gagggccgtt atgtccggct ccgggtgacc gacaccggca
     9541 gcggcatcgc gcccgaaacc atggaacgca tgtacgaacc gttcttcaca acgaaggaag
     9601 ccggaaaggg caccggcctc gggctctcca cggtctatgg cgtcatcgag cagcacaacg
     9661 gtttgattca tgtggaaagc caggtgggcc ggggcagtgt tttcgacgtc tatttccccg
     9721 tggcccgcag ggacccggag cggccggccg gtgcggcctc gtcgccgtcc ggcaacggcc
     9781 gcgaaaccat tctgctggcg gaggacgacc cgtcggtcgt gggctacacc cggcgcgtgc
     9841 tcgagcgggc gggatacacc gtcctgaccg cctcaaccgg cgtcgaggcg gtggagttgt
     9901 tctcccgcag cacgggccgg gtgaatctgg tcctgatgga tgtggtcatg cccggcctca
     9961 ccggcgtggc cgcccgcgat gaaatgctga aaatccgtcc ggacattccg attcttttca
    10021 tcagcggtta cagtcacggt gccatccgtg ctgaacggat cgcgaaggag aaaatcgaga
    10081 tcatccagaa accgttccgg gcatccgatc tgctggcccg gattcgggaa atcctcgacc
    10141 gccgccccgc ctgaaaagtt ccaatccctg gaactttctc ccgaaaaact tccaatcatt
    10201 ggaacttttc gaccggaaac ttccagtcat tggaactttt tgaccttcga gaaaaaaaca
    10261 cggcgttcga agtccgtggt tggcaagccg gaatatggat gctagattcc tgcaactatt
    10321 gtcaaccata gcaggttcag cctgtcagga gaaatgaagt tatgacgatt accgcgaaaa
    10381 agatgttgaa agacaacccg attctgaccg acgcgcagct gcgcgccgag gtcgagaaat
    10441 gcgaattctg cgaagagaag ccctgcaagg ccgcctgtcc ctgcgactgc tcgcccgcag
    10501 atttcatcat ggccgtgaag ctcgggacac cggcggattt caagcgcgcc gccgctgaaa
    10561 tcatgtccaa gaacccgttc ggcggcatct gcggcatggt gtgtcccgac acgctttgca
    10621 tgggcgcctg cgtccacaag aagatggact gcgcggtgaa tattcccgcg gtgcaggcga
    10681 ccatcatcgc gaaagcccgt gaactgaaat ccttccccga attcgaaaag gtgaagttca
    10741 acggccgcac ggtggcagtg gttggcgcgg gtccggccgg acttgccgcc gccgcgacgc
    10801 tggcccagaa gggctacaag gtggatatat tcgagcagaa cgaaaagagc ggcgggatgt
    10861 gccgtctgat tcccgagcac cgcctctatc gcgaaatgct ggagaaggac gttgatttct
    10921 gcctcggcat gggcggcagc cgcatcaaga tgaaaaccgg caaagcggtg tcggatccga
    10981 aaacgctcct gaaaaaaggc tacaaggcgg tgattgtcgc ggccggcctt tgggaacccg
    11041 tcatcttgaa cattccaaac aaggacaagg cgattggcgg cattgaattc ctgcagaagc
    11101 ccaaatcgtt cggcctgcgc gggaagaaag tggccgtcat cggcggcggc gccacggcgc
    11161 tcgactgcgc ggtggcggcg aagctttccg gcgcgagctc ggtcgagatg atcgccctgg
    11221 aaaacgtcgg cgaaatgccg ctcaccccgc gtgaaaagca ggatctggtc gagcacggca
    11281 ttgacttcaa cggccgcatg cgcgtcaccg aaatctgcgc caacggcgac aaggtgaccg
    11341 gcctcaagac gatcaagatc gcacttccgg ccggcgagaa attcagcgtc agcgcgctga
    11401 aggacatcaa gggtaccgag cagacccgcg cggacatcca gattgtgatc atggccatcg
    11461 gcgcgcgttc caccattccg aaagtggatt cccgcggcgt gttctacgcc ggcgatatcg
    11521 ccaacggccc gacctttgtg gtgaccgcct ccgcggccgg aaagaactgc gccatggtgg
    11581 tggatgccgc gctgaaccag cagaaggccc cggtcatcaa ggatccgaaa aagagcaccg
    11641 tgctggtgga aggctacaac atggtcccgg tttcactgga aaccgatttc ttcggccgca
    11701 agctgatcaa cccgttcctg ctgtcggcct cgccggcttc ggacggatat gagcagatga
    11761 agaaggccta tgaggccggc tgggcgggcg gcattatgaa gaccgccttt gacaacctgc
    11821 cgatccatat tccggcggcc tacatgacct gcttcaacga cacgacctgg ggcaactgcg
    11881 acaacgtgtc cgatcatccg attgaccggg tctgcaccga ggtcaaaaag ctggtccggg
    11941 aatatccgga ccgcctgacc ggcgcgtcca cgggaggcaa tgtctccggc aatgacgaga
    12001 gcgacaagaa gagctggcag agcaacaccc gcaagctgga aaaagccggc gcgatggtga
    12061 tcgaatacag cctgtcctgc ccgcagggcg gcgagggcgc cgaaggcgac atcgtttcgc
    12121 agaacgcggc cctgacggcg aagatcatcg gatgggtgct ggaagtcggc gatccctcgg
    12181 ttcccaagct gttcaagctc accagcgccg tgacggacat caagagcatc ctgaaggcgg
    12241 tgaagccggt ctttgccgcg tatccaaagg caaaggcggg cgtcacgctg gccaacacct
    12301 tcccgtcgct ggcgttcaag cccagcccgg caaagaaggt ctgggaagac ggtgtggttt
    12361 ccggcatcag cggcgagggc atcacacccg tgagctatct ctgtctggcc agcgccggcg
    12421 gcatgggcgt gaccatctcc ggcaatgccg gtccgatgaa ctacaaagcg gcggcggact
    12481 tccttgcgct gggcaccaac acagtccagt tctgcacggt ggttgaaaag cacggctatc
    12541 tgatcattga cgaactctgc tccggtttga gtcatctgat gcaggcccgc aagatcaagt
    12601 cggtcaagca gcttgttggt attgcccagc ctgagccgat ccgcggcttc atggacctgg
    12661 acgggaccaa gcagatttcg acctgcagca cggacctgtg cgtgcagtgc ggcaactgca
    12721 cccgctgtcc gtacctggcc atttcgatga acgcggacaa ctatccggaa acggatgcgt
    12781 ccaggtgcat cggatgcagc ctgtgcgtgc ttcagtgtcc cgcaaaggcc ctgagcctgc
    12841 gcgaccggac agcaaaggag aagaaggcgc tgcatgcctg accgcgattc ccgcgtcggg
    12901 cggccggaaa cggaccgccc gccgtgtccg gaaaaatgac cttgctctgg accggtgcag
    12961 gcagtaactt ctccctgttt tccatcggaa tgctcgggtg gtgaaatggc agacacgcca
    13021 ggttgagggc ctggtggccg caaggctgtg cgggttcgac tcccgccccg agcaccatgc
    13081 gcgctatttc atgaagccat gtcggatcca gcgttcccgc gtccggtttc tgatccaccg
    13141 cgcggttcca attcgatcat cgccggtcag gccggccgcc cgcgcatagg cgcgccagaa
    13201 acacacccgg tcagccagag acggttcgcg gtctgtattg aactgcatca gattgtgatt
    13261 tctcttccgg cgggggagcg tccgatattt ccggttgcgc tcgttgtcca gccagatgaa
    13321 ttcccagcct tcgccggttt cgcgcgaaag gatgttgttc gtccgaagat ccccgtgaca
    13381 gattcccgcc gcatgcaggc gcgccgcggc ctccgccacg gcccgaagga aggcaaagcg
    13441 tcgggatgtt ccggccgcaa gcccgtattg cggatccatc agccgttttt tcaccgaaac
    13501 cgcattcgac acctcgtccg tgatcagcat gctttcactt ctgaaaaatc cggccggccg
    13561 gatcaggcag cggacatgcg gggcggagaa cccggacttc tccatcagca ggtcaccccg
    13621 ccaggcccgg cccgccctcg aggttctgaa catccccttg aaggcatcgc gccatccccg
    13681 gagcaggaat ttcttcagat aaaccgctcc ggtctcgctg ctcagcgctc cgcgccgaac
    13741 tttggaatac ggagaacagg tgaccggctc aaaacccgga agcttcatct cccagcagtc
    13801 cggcgcccgc gccgcccagg aggcctcgcc atcccggaag gtgaccttca gccggcgccc
    13861 gcccgccgca ggaacaacct gcgtgatcca gatgcggttt gtgcttcgtt tcatgcgggg
    13921 tactgtgtaa tgataaccgc tgtttagcaa gtcagttcgg ttgaaggacg acaggcgttg
    13981 agcaaaactc atttcataaa gtccaccgcc attgtgagcg gtttcacggc cctgagccgc
    14041 gtgcttggac tggtgcgtga catgctcatg gcgggagtgt ttggaacctc cctgtatatg
    14101 tcggctttcg tggtggcgtt caccatcccc aacctgttcc gcaggctgtt cggcgaaggc
    14161 gcgctctccg cgtcctttat ccctgtattc atcgaggtcc gtcacaagga gggcgaaggc
    14221 cccgcgtgga tcatggtgcg caaggtggtc actctgctga cactgaccct gctggcaatt
    14281 gtcatcgccg ccatgctggt cgtgaccgcc gcgtcgttgt ggatggatct cggcgaaaaa
    14341 gcggcgctgg tgatgccgct gctcaggatc atgctcccct ataccgtgtt catctgtctc
    14401 gccgcgctgt ccatggcgat tctcaactcc tatcatcact atgccgttcc ggcctttacg
    14461 ccctgcctgc tgaatttcgt gtggatcgtc gccgtgcttt tcatctgtcc gaggttcacg
    14521 ggagacccga tggggcagat tggcgctgtg gcctgggcgg tgattgcggc cggagtgctt
    14581 cagctcggcg tgcagattcc gcagctggtg aaatacggat atcgcccggg tctgtcgttc
    14641 gactggaaag accgccgcat gattcgcatc ctcacgctga tggggccggc cgcgctgggc
    14701 cttgccgtta cccagttcaa cgtggtgatt gaccgcctgc tcgcgatttg gatcggcccg
    14761 tgggcgccgg ccgccctgtt cttcagcgaa cggctgatct atcttccgct cggcgtcttt
    14821 gcggtggccc tgagcaccgt gctgcttccg actttttcgg gccatatggc cgtcacggac
    14881 acggttcgaa tgaagagcac cctcaaccgg gcgctgcgca atctgctgtt tgtgatgatc
    14941 cccgccgcgg cggggcttct gatcctttcc ggccccattg tggagatgat attcgagtgg
    15001 ggcaacttca cccggcagtc cacccggctg acggcgcttg cacttcagtt ctatgccccg
    15061 gggctggtgg tgttcagcct ggccaaggtt tttgttccgg cctattacgc catgcaggac
    15121 acccgtactc cggtccgcat cgggctctat gccgtcctgc tgaacctgat tttaaatgtc
    15181 acgtttgttc tcacctggcc gctgtactgg aagcatgcgg gccttgcgtt cgccactgtg
    15241 ctcgcggaag gtttctatgc cctggcgctt gccgtggtgc ttcatcggcg catcgggtcg
    15301 cccggctggc ggtccatcgg ctcgagtgtt tccttatgcg ccgcctgcac cgtcatgatg
    15361 gggcttacgg ttgtttatgt tcactccgcg ctgactctgc atccgggcat tcttccgctg
    15421 gatggaaaac tgatgcaggt tgcaaatgtc ctgatttcca ttgccgcggg catggccgtc
    15481 tatttcggcg catcggccgc gctgaaatcc tccgaactgg ctgatgtggc ggcggccctg
    15541 cggcgccgga agcctcaaac cagttcgaca aacacctgat tggatacaaa aagcgccgac
    15601 tccgtcagtt gcagaaaatc cccatcctgt ttcagcagtc cgtccgcgat cagcttctca
    15661 atggccggtc cgcagagtgc ataatagtca aaacccgtct gtttccggaa aaggctctgc
    15721 gagacaccgc ggattttccg gaggctcatc acaagcgtct cgcgcgcccg ggcctcgggc
    15781 gccagccgtt cctcaaatgc ccggcgcggc ccgtgcagga tgtagtcctg cgtgtctttc
    15841 tcgtttgccc ggcgcgtgcc gttccagtgg gaatgggccg aaggtccgca gccaatgtat
    15901 tctccgcctt cccagtacag cagattgtgg cggcattccc gtccggtccg ggcgaaattt
    15961 gagatctcat aatgctcata acccgccgcc ccgagcgttt ttaggatgag ccgatactgc
    16021 gccagcgcct cttcatcatc ggcttcggac acgctcatgc gcgcgagcgc cgtgccgggt
    16081 tcgatggaaa ggcagtagca ggagatgtgt tccgggccca gttccagaag ccggttcagg
    16141 tcgttttcca cctgccgggt gcttccgccg ggaagcccat agatgagatc cgcgccgatg
    16201 ttctggaatc cggcggttcg cagcgcccgg aaactcttca acgcctcctc tccggtctga
    16261 atccggttca gaaacttcag cagcttttca tcaaacgtct gaatgcccag cgacacccgg
    16321 ttgacgccgc cgttgagcaa aacctgaatt tttccttcgg tcagtgttcc gggattggcc
    16381 tcgcagctcc attcctcaac acggcctgta ttcacgcggc cgcgcaccgc gcccagcagt
    16441 cgcgaaagga cgtcctcagg cagcgccgta ggtgttccgc ccccgataaa aattgtggct
    16501 ggtgaaaaat cctccggcag gctgtccagt tccgattcca gcgcatcggc aaacgcccgg
    16561 gtttccccat ccgtcatggc ctgcgaatgg aaatcgcagt agctgcattt ccggaggcaa
    16621 cagggcacat gaatgtacag cccatagttc atgccgcgga tcatatcaga aagtcttgag
    16681 aaggcacctc cggatatcgt atgtttcccc ctttcggccg gcgaggccgg ggcggtcagc
    16741 aacagagaga agctatatga agaaaaatta tgttctggac accaacgttc ttctccacga
    16801 tcccaatgcc atcaacagtt tcaaggactg caatgtggtc ataccactca ccgttcttga
    16861 ggaaattgac aatttcaagc gcgagctgac cgagcgcggc cggaatgcgc gccagctgtc
    16921 gcgccagctt gatgagctcc ggcaccgggg gcgtctttcc gaaggcgtgg cccttgacaa
    16981 cggcgggggc ctgcgcgtcc tttttcacgg ggacctggag gcctttgccg aatcgcgcaa
    17041 gaacgacgac attatcctga acgccgccct tgatttgaag aaaagcgcga ctgagcccac
    17101 catcctgatt tcaaaagaca tcaacatgag gctcaaggcc gacgccattg gactggatgc
    17161 cgaggattac gaatcgggac aggtcaaatc gccagagttt cactgcggct acattgagct
    17221 tgaagtggac tcgcagtcat tcagccggtt tgagcatgag cacaagcttc cgatggaggg
    17281 ggaagagctg aacccgaatt tgtatatcgt gcttgaagag gagagcaatc ccggacatca
    17341 ggcgctgggc cgttatgacg ggaaaacgaa aagcattgtt ccgctgattg cgctgccttc
    17401 gcagatgcat cccattcatc cccgcaacac cgagcagcgc tttgcgatgg atgcgctgat
    17461 gaacgacgat atccgcctcg tgacgctcac gggaaaggcg ggcaccggaa aaacactgct
    17521 ggcggtcgcc gccggtttct tcaagaccgt ggacgaaggc acctacacca agcttctggt
    17581 ttcgcgcccc acgctgccca tgggccgcga tctgggttat cttcccggcg atcttgagga
    17641 gaagctgaac ccgtggatgc agcccatcca ggatgcgctt gacctcgtgc ggacggggag
    17701 aaaagccgga cagaaatccg accgcgactt caccatgttc ggcgacaaga tcagcgtgga
    17761 acccctgacg tatatccgcg gccgcagcat tcccggccag tttatcatca ttgatgaaac
    17821 ccagaacctg acgcctctgg aggtcaagac catcatcacg cgcgtgggtc acgacacgaa
    17881 gatcattctc acgggcgata tttatcagat cgacaatccc tatgtggata ttctgtcgaa
    17941 cgggctcaac gcggtcgcgg agaggttccg tccgcacgcg gtggcggccc atgtgatgct
    18001 gaaaaccggt gttcgctcgg aactggccga actggctgcg aacatcctgt aggagatacc
    18061 catgaagaaa atcatcagca ttgcgcttct ggcggggctg ctgctctgcc cggcctcccg
    18121 ggcccgggat gcggcgctgg tccggcagca gtacgacatg gtcaaatcca gactcgatga
    18181 cggcggcgat ctgatgattg tcgccaatgt ggacgggctg gttgaggcgg gcgtccagaa
    18241 acttgtcgag atggcttcga tggccgcttc cacacagccg tctgcgccgg gcctgcctgc
    18301 cgcgctggaa aaactgcccg cttttctgaa ggcgaacgga ttttatgccg tcacggccgt
    18361 tggattcagc gcggtgccga aggacaatgg cctgaacacc atcaaggcct tcgttggccg
    18421 agatgccgcc gccgccgccc ttcctctgtg gcgcggactt ttcgggctgc agggtattga
    18481 aatgatcacg ccggcctatc tgcccgccga caccgagatc gtccggacct ttgccgccga
    18541 tccggagcag ctctggaagc tctcgcgcga ggccgtgacc cgcctgggaa ccccggaagg
    18601 cgccatggcg tttgaacagt cgctggcaat gtccaccatg atgctgggcg taccgctgga
    18661 caccttgatc agcagcctgg gcggcgaagg cttcctttcc atgcagctgt caaagaccgc
    18721 aagtgcggaa cttccgctcg gcggcgccgg gaaaaccatg acagtcgctg aaccctcggt
    18781 gctgctgggt ttcaccgtga aggatgaaac gctcgccacg ctgatcaaaa cccgtttctg
    18841 cggcgagggc ctcccggccg ctttgaccga aaaagacgga gcggaaatca ttactatgag
    18901 catgaagctt ccgtcgccga tcccgatgca gatcacgctg gcggttaaga gcaatgtgtt
    18961 cctgatcggg tcgaatcccg gcgtgatcaa ccgcgcgctg gcggcggcag agtccggcga
    19021 agggctgctg gcctccgaaa aattcaaaac ggcatttgga tcacttccga tgaagaacaa
    19081 cggaatgatg tatttcagtc ccgaatgcgc ggaattcttc aagaccattc agcaggccgc
    19141 cgtcatgaac gacagcggag cggatgccga catgaacgcg gccatcatga agttgttcgg
    19201 aagtgacgag ccccagcaga gcgcgatggt cattctgaac acaagaaatg gaatccagct
    19261 tcagggaaac accacattca gcggccggga tgtgctggcc ggagccgtgt ttgcgcccgt
    19321 gggcatcgtg gcggggatga tgctgccggc cgtctcatcg gcccgcgcga aggcccagac
    19381 cgtcgcctgc atgaacaatc tgcggcagat ccaggccgcc aaagaccagt gggccattga
    19441 aaaaggaatg gaacgcggcg cgccggtgtc cccgtccgac atagaaccct atttgaggga
    19501 aattccaacc tgcccggccg gaggaagcta cattctgaat gccgtgggtg aagagccaac
    19561 ctgcgaccat ccgggccacg gaatgaagtg aggcgcccat gttgaccggc ggatatttca
    19621 tttttttcat tcccgcgatg atcctttcga tgatcgcgtc cgcgatgacc aaaggaacct
    19681 tcaggaaata cgcgagggtg gcggcttcgt ccggcatgac cgggggcgag gcggcccggc
    19741 gtcttctgga ctcgcagggc attgacagtg tcagcgtcaa ccacatcggc ggcttcctga
    19801 ccgaccacta tcatccgctg aagaaagaac tcaacctttc agacggggtc tacgggtcca
    19861 gttcactggc ggccatcggg gtggcctgtc acgaagccgg ccatgcgctg cagcacgcgc
    19921 acggctacgg cccgctgatg ttccgctcgg ccatggtgcc ggtcacgcag ttcagcagca
    19981 ggatatccta tctggtcctg attctcggcc tcgccatgaa cttccgtccg ctggtggaca
    20041 ttggcatcat cctgttctcg gtcgcggtgc tgttttcact ggtgacgctt ccggtggagt
    20101 ggaacgcgag ttcgcgcgca aaaagactga tggtttcatc gggcattgtg accgcggccg
    20161 aagaagacga tgccggcaaa gtgctgaacg cggccttcct gacctatgtc gccgcggcgg
    20221 tgtcgtccat catgacgctt ctgtactaca tctcgcgccg gcggtagttc tcacagcagg
    20281 ccggttgcgt aggcgatcag aacgggaatg gagaagattc catccacctt cgccagtcgc
    20341 tcgccggtat tcggtttcat caggtaggtg ccgtcaaact tgaaaagacg ttctccggtg
    20401 ttgggtttca taatgtaagt gccgtcccac ttgtacaggc gttcaccggt gttgggcctc
    20461 atcaggtact ccccatccca cttgtagagc cgttcgcccg tgttgggttt catgatgcag
    20521 gtcccatccc atttgtacag ccgttcgccg gtgttgggct tcatgagata ggttccatcc
    20581 cacttgtagc ggcgctcgcc ggtcgtcggg gatgtaatgt atgaatccgc aatcgcgctc
    20641 accgcgacga tcatcgcaaa caatgccaaa attaatcttt tcatggcttc agcataaaac
    20701 tccggccggc acgagtcaac cggcgcaaaa caaaacttcc aatgattgga actttctgtc
    20761 agattttttc caatgattgg aacttttttc cgtaaaactt ccagccattg gaagttttgc
    20821 agtcaggaga gattatgctg gatttcagga catatcagga gcgggcaaga agcacggcgg
    20881 tgtatcccgg catgggcgag ggcaactgga tttatccggc gctgggactg gccggcgaga
    20941 cgggcgaaat ctgcgagaag atcaagaagt gcatccgcga cgacggcgga aagatgaagc
    21001 cggaacggct cgcggacatc gaaaaggagc tgggggacgt gctctggtac atcgccgcgc
    21061 tcggcagcga gctcggcctt gacatggaaa ccattgccga gcgcaatctt gcgaaactcg
    21121 cgcaccgcaa gaagaacggg aaaattcacg gggacgggga tcatcgctga tcatgaagat
    21181 tgatctccat gtgcatgcct ccgagcgttc cgcctgcagc gtatccggcg aagaggacat
    21241 gatccgcgcc gccgccagac gcggcctcga cgcgctggtc ttcatggatc accactgcct
    21301 ggtgccggtg agccgcctgc aggagctgaa ccgggagttt gcccctttcc gcatcttcgg
    21361 cggcgttgaa atcacgctca gtgaggagga ggacattctg gttctgggtg tgcgcgatcc
    21421 aaggctggaa cagcgcaact ggaccatgcc cgacctgcac tctttcgtcc gcgaccgcgg
    21481 cggctggatg gcgcttgcgc atccgttccg ctacagggat aacgtcaact ttgacatgga
    21541 aaaatatccg ccggacgcca tcgaggtgcg ctcctcgaac atcgcggccg ggaacctgaa
    21601 gcgaattgaa gcgctgctcg gactgacggg cctgcccgcc atctgcacct ccgatgccca
    21661 cattgcgaac cgggcgggtc tgtactatgt ggagctggac caaacccctg cggacgagac
    21721 ggagcttgcc cgcctgctgc gcgcgcgaaa gttctgcgca tgaccgggga tgaaatattt
    21781 ccggtggtgg atgaaaacgg aacggaaatc ggccgcgccc ggcgctctga atgccacgga
    21841 aatcccgccc tcatccatcc ggttgtgcat gtgatcgtgc gcagccgacg gggcgaaata
    21901 tttctccagc gccggaacat tcgacgtgat gtgcagcccg gaaagtggga cacatcggtt
    21961 ggcgggcaca tgcatcccgg agaggaggcg gccggggccg cccagaggga aatgcaggag
    22021 gagctcggtg ttcgcccgcc ccggctgcag ttcgcgtacc gttatttgtg gcgctccgaa
    22081 acggagtccg aactggtcca cacgtttctt gcggaacatg aaggaccctt catcctgcag
    22141 gccgaggaga ttgacgaagg gcggttctgg actgagggtg aaatcagggc cgctgtcgga
    22201 tcgggaaagc tgaccccgaa ttttgaggcg gaattcgaaa ggctggtgaa acatgaatct
    22261 tgaccgggaa cttggggtgg cactggaagc ggtgaaggcg ggatgcctgc tttcccgcgc
    22321 agtgcagcag ggaatcactt ctgtcgcaaa agaggaccgg tcgcccgtga ccatcgccga
    22381 tctcggcacg cagtgcgtga tttccatgcg cttgatgaat catctgcccg gtgaaacgct
    22441 gatcggtgaa gaggacacaa agatatttga ggggcgcgag cagttgaagt cgcaggtgct
    22501 ggagctggtt cgccgcttcg agccggatac ggatgaggat caaatgatgg gggcgatccg
    22561 cttcggttcg cagcccgcgg ttccggaacg gttctgggtg ctggatccga ttgacggcac
    22621 aaagggtttt ctgcggggcg accagtacgc catcgccctc gggctggtag tggacggccg
    22681 ggttgttctc ggggtcctgg gctgcccgaa ttacggcgaa agaatgttct atgccgtgac
    22741 gggcagggga gcctttgcag tccatctcaa tggcggagat aaaaagcaga tcaacgtgga
    22801 tgatgttgcc gacccgtcac gggccgtatt ctgcgagtcg gttgaatcgg cgcacgccgc
    22861 gcacggcgac cacgcgaaga tttcagaaat cctgggcatg acgcgcgcgc cgttccggat
    22921 ggacagccag tgcaaatatg cggcggtggc gcagggcgat gcctccattt atctccggct
    22981 ccccagccgc aaagactatc aggagtgcat ctgggaccat gcggcgggat ccattcttgt
    23041 tgaagaggcg ggaggcaggg tcagcgactg cgacggcaag gctctggact tcacggccgg
    23101 ccgccgtctt gtgaacaacc gcggcattat cgccaccaac ggccggattc acgatgccgt
    23161 gctggccgcc atcgctcagc tttgatttat ttgttcggca ccgggccgac gatccacggc
    23221 gcagcttgtg gatcgtccgg aagagtaatg cggagagaac cctgaaggtc tgtgcgccag
    23281 cgctcgatgt ttcgggagtc gagccgttga acaactgtcg gagagggatc gccgcgccgg
    23341 ttgtttctgc cgacggaaat gaccgcgagg cgcggtgaaa ctgccgtgag ccattcatct
    23401 gatgtggtct tttccgatcc gtggtcgccc aggaccagaa tgcctgccgc gggctggatt
    23461 ccggcctgca gcatggcgcg ctcttcggat attcccgctc cgcccatgaa caaaatggcg
    23521 gcagccttgc gtccgaaccg catgaccagc gaggcctcgt tggcgatgcg gaagtttttc
    23581 gttccttccg gtgaaaacac ctcccagacc acgtcgcccg cggccgttcc ccgcaggccg
    23641 cgctccagcc gacggacgcg gattccccgc gcacggactt tttccagcat gcgttcgtat
    23701 ctttcagact gtcccttgaa ggggcagacc cagacttcgc cgacctcaaa acggtccatg
    23761 acggcctcgg cgccgccggt gtgatcggcg tcgggatgtg tcaggacaac ggtttccagg
    23821 cgatccaccc cctcggcctg cagatggcgg atgacgcggt tcgcgaagta gtgcggcccg
    23881 gtgtcgataa ggatatcccc gtctcgggga agattcatga agaccgcgtt gccttcgccg
    23941 acatccagca cgtcaatcgc cacatcctgc cggtaccatg cgagggagaa cgccgccagc
    24001 gaggcgatca atattccaca gaaaaaaatc tttctgaaac ccatccgcat gaaaaccagc
    24061 gcggacaaag tgatgtacca caggagcatc agaagcgggg agggcgactg gacatggctg
    24121 tgtccgtgcg ggatgctact catcaggcgg acgatccaca gcaggaggtc cacaaaaaca
    24181 cggttggcat gattgaaaat ctcggcgcac acagggaaaa gcgatcccgt gaccagagag
    24241 aggcagccgg taagcacaac gagaaacgat ccgggaacga tgaagatgtt tccgagcagg
    24301 ccgatgggag aacacagatt gaagtaatag gccgtcgtgg gggccgaaac cagccaggcg
    24361 gccgtggaaa cagcggcaag atcagccgcc ttttccgcga gaaaccggcc ccagcgctcc
    24421 agccgtgggc gcggtcctgg aatccaccgg ggtttccgga gcggaagagt gtaaagacgc
    24481 cggaagaccg gatagaaaag gatgatgccg gcaaccacgc tgaaagaaag aatgaaaccg
    24541 ggcgaggcaa tctgtcgcgg atcggctgcg agaatcagga tggcggccgc cgcgagggtg
    24601 gagcctccgt ccggccttct cttgaacagc gggccggacc agtatagaag agccatgatg
    24661 cacgcgcggg ccgcactggc cttgaggcct gtgcccatca cataaaggat gagagccggc
    24721 cccagcagca gaatccagta ctgtcgcgag attcccagca tcttgagcac ggcaatgatc
    24781 agaatggcca tcacgccgac gtgaagtccg gagatggcga agatatgcag cgttccggtc
    24841 agggcgaaca catcgcgcag gttgtcggga agggcgcttc gatagccgag cagaagggcc
    24901 tcaagcagcc cgcgaatatc cggataattt tccagccccc gggaaagaac gagagaaaat
    24961 cgcgcgcggc tggcatagca ccaccccttg agccggctgc cgtttccgcc ggagaggatg
    25021 cgggtccttc cgtcttcgcc ctccatggtt acagggggga gccaggccct gggtgatgcc
    25081 aggttcttca gcaggccttc aacgatcagc cggtcgccgt agcggagagg cggatcggct
    25141 tctggaagcg atatcctgac gaacaccctg ccgcgggcgg cctgccactg ctcccttcga
    25201 cgaacgccct ccagcttgag cgtgaagctc cagcttgtgc tgaagcggcc gaagccgggt
    25261 tcggattcag gatcgtcggt gacgaccccc atgagctgaa gatactcgcg ggaatggcgc
    25321 atcatgctcc gaacatctct gcgggagggg ctgtgatgca gaagcgaagt atgcgcggcc
    25381 gccgttaaga ccagcgccgc ataaacggcg gcgcccgccg ccggatgttt ccgaaagacc
    25441 atcgccgcca cggtcaggac gaatgccgtc acggccggaa gcaggaaaca ggggggaccg
    25501 cgcaggcccg cgagacagcc ggccatcacc gcgacagcca gaccgaccag cggccggcgc
    25561 gggcccggca gatattcgga aggcggcgaa gacttcatta aggcgtcaga gtccggggcg
    25621 ggcgtcgcgc aggttcagcg gggtgtgcgg cccgatccgg taggcccggt tcagggcatc
    25681 ggtcacaaag gactgcgcgg tgcgcacggc gtcacagatc agttcgccgc gcgccagcag
    25741 cgcggtcagg gctccggaga atgcgcatcc ggcgccgtgt ttttcggcca catgaatgcg
    25801 gggcacgggg aaaacgtatt cttcgccctc atcatagaga acgtcataaa cctcctcgcc
    25861 cggaaggatg ccgcccttca cgacgcaggc cacatcgtat ttttctccga tttcccgagc
    25921 ggcgcgctgg aggtcctcca gcgacgaaat ggtatgcccg cacaggattt cggcttcatg
    25981 cagattgggg gtgaccacac gggcctcgtg cagaagctca ttgcagagta tttcgatggc
    26041 atcggcctgg agcaggcgga cgcccgtgcg cgacaccatg atgggatcca ccacaagaat
    26101 ggggatgccc tggctgatgt ccgcatccac gacggcgcgg atgatgtcag cggaatagag
    26161 caggccggtc ttggcggccg caatgggaaa gctttcgcag accattcgaa tctgatccag
    26221 cacgagttcg ggcaggaggg gaacaaccgt tcgcacctcg gtggggctgc gggccgtgat
    26281 ggccgtgact gcgcaggttc cgaacacgcc catggcggaa aaactcatca ggtcggcctg
    26341 caggccggcg ccgcctccgc tgtcggaaca ggcaatggtg agcgcggcca ccgcggtgtt
    26401 attttgactc atggttccta tcctccatac atcccatctg tgcgcattct aggcacaacg
    26461 cgcgggcaaa atccattgca tttttcctat tccccgccgc cggtcactcc ggcccgcatg
    26521 cgcttctttt ctccctgatg cctcttgtcc tccagcatgc gctgcttttg cctccggctt
    26581 ctgcgccgct tctgtctgcg gattttttcc gccgcctgcc ggcgggctga cttccgtttt
    26641 tcccgctgct cttcgaggcg gtcgcagagt tctctgcggg ccaggtggcg gttcagggcc
    26701 tgcgagcgtt cccgctggca tttgacttca atcccggtgg ggccgtgaat cagatatacg
    26761 caggaggagg ttttgttgat tttctgtccg ccccttcccg cgccgcggac gaacttttcg
    26821 gtcaggtcat cgtcacgaat accgagcgcc tgcatccgtt tccggagcag ctctgccttt
    26881 tctctgctga cgggcgatac agtcatattt ctcaccgggt ccggcctggt tcaaataaat
    26941 ccgacgtagg ggcgcgaaac gagcttttcc ggaatttctt cgacgaagcg cgaagtgatg
    27001 cagaactgcg tgctgccgtc ccgcccccgg cgcacttccg gaacgcagag gaacagttcg
    27061 tccttcgcgc gtgtggtggc cacatagaac agccgccgtt cttcggacac gccgccggga
    27121 acttccagcg acttggctga cggaaacatg ccgtcggcca gccacagaat gaaaatcacc
    27181 ttccattcga gccccttggc ctggtgaatg gtgctcaggc gaatcacccc cttgccgtca
    27241 tcggccggat ggtcgtcttc cgcatcgagg ttggaaagca gcgccatttc gctcagaaaa
    27301 tcatccggag ttgcgaactg cgacgcatag ttcatcagtt cctgaatatc ctcctgccgg
    27361 cggttgtaat tgtcgaaggt ctcgacggcg tattcgtcat agaacccgtc cacgaaggcc
    27421 tccatgtagg cttcggcgcc cgcgccctcc ggatcgcggg tgatttccgt gatgacggtt
    27481 tcgatggcct gccacgattc ccgcgccggc gcaggcagtt tggcgcgcac ggccgccagc
    27541 gcctgttcgg acgcgtcgaa gcggccgccc agcgactccc agattttcgc ggcggaacgg
    27601 cgtccgacgc gcggaagaaa ctccaggatg cgcaggaagg aaagctcgtc aaccggattg
    27661 cggaggaggc ggacaatggc gcagacatcc ttgatgtgcg cctgctcgaa aaaccgcgcg
    27721 ccggaagtga tgacataggg gatgcgctcg cgggtcagtt ccatctgcag ttccatcgcg
    27781 tggaaatgcg agcggtagag cacggccatc tcggaggttt tatatcccga ccgcttcagg
    27841 cgggcgatct gctcgataat gtagcgggcc tgcgcgctgc cgtcacgcag cacggccacg
    27901 gagggcacct tgtagttttc ccggacagac cgcaaagttt tctggaactg ctcgggattg
    27961 cccgcgatgc aggcgttggc cacgttcaga atttccggca cgctgcggta gttcgtctcc
    28021 agcttgaaaa cctttgcgcc ctgatagcgt tcgggaaagg aaaggatgtt ccggaaattc
    28081 gctccgcgcc aggaatagat gctctgaaaa tcgtcaccga cgacgaacag gttgcggttt
    28141 tttgacgcca gcaggtccac ccattccgac tgcaggtagt tggtgtcctg gtattcatcc
    28201 acaaggatat gctgaaactg ctcctgatag gatgcgcaga ttccgggctc ctgccggaac
    28261 aggcgcagca cgttcagcag catgtcgtca aaatccatgc cgttaagcct gcgcttgcgt
    28321 tcttcatagg cttccgccac ggcgatgacc tgctcgggat tgatttcatc gtgatacttg
    28381 aagcgctcct cggcgacaag ccgcagagat tccccggtgc tggccgcgta gccaaacagg
    28441 ctcagcagca catcgggctt tggaaaatgc ttgccggcat agtccagctc ctccgaaatg
    28501 gccttcacca gtttgcggga gtcgtcctgg tcgagaatgg tgtaatccag cccgtagccc
    28561 agcctgtccg cgtgccggcg caggatgcgg ttggccatgt gatggaaggt tccgccccag
    28621 aggccgccga cagcggctcc caccagtttg gccgcgcgtt ccagcatttc gcgtgcggcg
    28681 cggttggtga aggtgagcag caggatgcgg cgcgcgtcta tgccgcgctc cacaagatgg
    28741 gccacgcgat atgtcagtgt gcgggtcttg ccggttccgg cggcggcgat caccagcacg
    28801 ggcccgtcgg gcgccagcac ggcgcggcac tgctcttcat tcagttcact gctgtagtca
    28861 atcagactca tagatatttt gtgtttgtaa aaatttcctg cggagccgga ccgggctcaa
    28921 accagcccgt caatcagatc cagcaccgat ttgttttcca gtccggcttc gagggcgcgc
    28981 ccgtagcggg ccatcacggc aggaaccttg cctttcagac ggtcgagccc cagatattcg
    29041 gcggaaacgc cggcatccat gatcacgttg atgacgttct tcaggagaag ccgctccggc
    29101 gctttcagga gcacataggc atcctcgcga tccgccacgg gcgacagcca gccggcctcg
    29161 gtatagagct tgacgacatc atacagcagg cgcacgggga tgaggtgttc ggcggcgaag
    29221 tccttgatca cgaggcatcc atcgcccttt tccagccgct cggcggcggc ggtgatgagg
    29281 gccatgccga gagtggcgcg tgcgcgcagg ctggcccttt ccgattcgcg ctcgatgtga
    29341 taggtgtcat ggtgctgcac cgcgaaggcg atttccgcgc cgagcaggac gatcacccag
    29401 ctgatataga cccacgcgag gaaaatcggg atggaggcga aagtgccata aatggcattg
    29461 tagcgggcga cgcccacctg aagggaaata taggtcttct gccagccgag ccagagcagc
    29521 gcgccgatca ggctgcccca gaaggcgggc caggccttca cgcgcgtgtt gggcagaaaa
    29581 acatagagga agctgaaggc cagccacgcc gaaaaaacgg gagagagttt caggaacccg
    29641 gtatacaccg ggccgatcga gccgaggttc tgtatcacgc gttcgctgtt cagcgtggcc
    29701 atgaccgtgc ccgccacgcc gatcaggatc ggcaccacca ccagaatgct gatgtaattg
    29761 gccatgcgcc ggatcaggtt gcgcgaaatg gcgatgcccc agatgcggtt gaaggaggcc
    29821 tcaatgctgc ccagcaccgt gatggtggcc agcagcagca cgccgaaacc cgcccagccg
    29881 agcgccgcga aattcgtgtt ttggacgatg tccatggttt ttacaatgaa atcctgaaac
    29941 tgctgcggca tggtggtctg ccactccatg aaccggtcca tgccggcctg tcccgccccg
    30001 aaacccttga gaagtgaaaa aacgatggcc agcatgggca ccagcgacat cagcgccgtg
    30061 taggtcaggg ccgaggcgcg aattgaaagc tcgtcatcaa tgaacccctt gatgacgaga
    30121 tggccgatgc gatacactct caccgggatg gcgcgcagcg cgggcaggga ggacacttcc
    30181 atgtcccaca catcgcggaa ggcgaactgc ttcagccgcc ggcatttctg cttgaattcg
    30241 ctcatgttca tattgtctcc catcaggggt tcgggaacat tttctcatac agcagccagg
    30301 ccgcaagcgt tttcgcgtcc tcgacggcgc cgcgccggat catatccgtg atttcgccgg
    30361 ccgaaagaac aaccgtttcc acatgttcat catgatcaag gcgggtggcc gccggcccgt
    30421 cagcaagccg ggcatagaag agctgaatat gctcgtccat gcacccgacg gacgggcaaa
    30481 ccgttccgag gcgaacgagt tcttccacct catggccggt ttcttcgcgg acttcgcgcc
    30541 gcgcgcaggc ttcgggggcc tctctctcct ccagcagccc cgccacgatt tccagcacct
    30601 cccgttcgac gggtttccgg aactggcgaa ccaggacaaa gcgcccgtcg ggaagccggg
    30661 ccagtaccgc cgcggccccg cggtgccgca cgacttcccg ggtggagcgc accccgtttt
    30721 ccagctccac atccagcacg tccaccgtca gcaggcgtcc cttgaaaact gttctgcttc
    30781 cgattgttgt ttcgcgcata ctttctgctc ccgaacggat tgaatcacaa accggggcga
    30841 aacttcaatt cgcgtcttgt gaatccgaaa aaaacttgct tttcccattg tatgaccggc
    30901 gcgttccgcc tatatccaga gagtcggaga ttcatcatga taaaagaaac gatagccaaa
    30961 cttgtggacg ggaaagagct gacccgtcag gaatcctatg aaacgctgaa ggaaatcatg
    31021 tccgggaatg caaccccggc gcagatcgga tccttcatca cggcgctgcg catccgcggc
    31081 gagtcggcgg acgtcattgc cggcggggtg caggcgatgc gcgagaagct gatcgccatt
    31141 gccaccccgg agggcaacgt ggtggacatc gtgggaaccg gaggcgacca gcagcacacc
    31201 ttcaacatat ctaccacggc ggcgtttgtg gtggccggcg cgggggtcat ggtggccaag
    31261 cacgggaacc ggcgcgtatc gagttcctgc ggtgccgccg atgtgctgac ggagcttggg
    31321 gtgaacattg agattggacc cccgcagatg gaaaaatgca tccgcgaggc ggggattgca
    31381 tttctttttg cgccggcgct gcatccggcc atgaaatacg ccagcgcccc ccggtccgaa
    31441 gtgggcatcc ggagcatctt caacattctc ggtccgcttt ccaatcccac cggcgctcgc
    31501 cgcggggtgc tgggggtcta tacgcgggat ctggtgaagc ttttttcggg cgcgctggcg
    31561 cagctgggtt ccgtgcacat gttcgtggcc catggtttgg acggtcttga cgagatcacc
    31621 accaccggca aaaccgcgat gggagaagtt cgtggcggag acatccggct gtttgaactg
    31681 gacccccgtg actatggaat tccgctgacg gattccgcca gcctcagggg aggggattcg
    31741 gcggcgaatg cgctgctggt gaaagaggtt ctgtcgggga aaacgggtcc gaagcgggac
    31801 attgtgcttc ttaacgcggc agtgaccatc atggcgggcg gcggcgcgga gacaatagag
    31861 gcgggtctga cggccgccgc gaaatccatc gacagctggg cggcgatgga aaagctggag
    31921 aaactcgtgg ccctgaccaa cgagaaatag aatctatttc ttcgaaaaat tcgcctctcg
    31981 caaaacccga ttatcaaatt tgagaatatc tatcattttc taaaatatga ttatattgtc
    32041 ttcctgcgct tgattttctg aaatattgtg ctttcttcag gtaatgggcg ggttgtccta
    32101 atgatttcgc gtttttttat ccgccgattc cccgttggca tgcatcctgc ttaaaagtac
    32161 atcgatgcgg ctgtctgatg gcagcggcag agtgaatgaa gagttaataa aaagttatgg
    32221 agtatgaaaa tgaagaagag tggtttcacg cttgttgaaa ttatgatcgt ggtcgccatt
    32281 atcggtctgc tggccgctat tgcgatcccg aacttcgtca aagcccgcca gtcctcgcag
    32341 cagaagtcct gcatgaacaa cctccgtcag attgacggcg cgaaggatca gtgggccatt
    32401 gatgaaggca aaacgtcctc ggacaccccg ggaaaggccg atctggagcc ctacctgaag
    32461 tcctggccga tttgcccggc tgccggcgca tacaccatca acgcggtggg cacggaagct
    32521 tcctgctcca tccatggaac ggtgctgagc ccgacaaccc tgtaagggta attgtttgaa
    32581 caggcggccc ggaggaaact ccgggccgct ttttttccgc tttttacttt tctggccgcc
    32641 tccggccgcg ctacattcag cgcaacagga ggaagctgca tgagggctcg ggggttcact
    32701 ctggtcgaaa tcttgattgt ggtcggcatt gtcggtcttc tggccgttct cgcgattccc
    32761 tctttcatga agtcgcgtga aaaatcccag gccaaaacct gcatgaacaa cctgcggcag
    32821 atctactacg ccaaggagca tttcgcatcc cagaacggcc tgaacgcggg cgatcccgtc
    32881 acggagaacg acgtggcggt gtttcttaat cacggactgc ccgtctgtcc ggccggggga
    32941 gcctattctg tgaatcccgt cggctctgat ccatcctgca ccgttgccgg ccacatctgt
    33001 ccctgaatac gcaatcaaag aatggacacg cgcacacgtt tcttctaggc tctgcatctg
    33061 gaaagtggtt atgagaaagg acattttcat gaaacgcatt gccctgaccg cggttaccgt
    33121 gcttctgtgc ctgtccgcct ccgcctatga tacggatgcg gtcaaattca aggacggccc
    33181 gcacgccggt ttttcctttg gcggggatgt ccgcagcgag gacttcgcct ggggctggca
    33241 ggccgcttac gagcacagcg actggctttc cctggaagcc tccatcacgg cgcagaacga
    33301 cgaactcgag cagagcatca atgacgtggc cattccccag ccgtttggac ttgaactgga
    33361 tgtctatgcc ctcgccttct ccgcgcgcgc cggcattcgc ccatggtccc ggctgcacct
    33421 ctatgcgctg gcgggcgccg gctactatat tatgaagacc gatgccgaag aagcccgcgt
    33481 catcatcgcg ggtgccgaac cctccccgtc cggggttcgc ctgtccgggc ttacagtgga
    33541 tgtggagaac agctttggtc tgcatgccgg cgccggcctc gagatccggc tgtccgaacg
    33601 gtgggaaatc ttcgccgagt tccggcaggt gtttctggag gcggatgcgg atctgaagat
    33661 caccgaggag tggccgtccg gtgttccgaa caatccccgc atgatgcaga cgcggatggt
    33721 ggacgacaag ctcccctatg atcacagcct cgtgcgcgcg ggcgtcagct accggtttta
    33781 acccgtccgg ttacaggtcc agaatccggt tggtcggcag gtcggaatct ccggtggtgc
    33841 tgatatagcg cgtggtgcgg aattcctcga cttccttcat cttgtgcagg atttccgcga
    33901 tgcgctcggc cgcctcgcag ctcaggccga taatttctga aaacacttcg ctttcccgga
    33961 acttgtcctg cttgagcatt tccagattgg caagcagcac ggtggccggc tgccccaggt
    34021 gatggcaggc cgcgccgagg ctggccagca tggcccggtg ctgttcggcc agccgcatgc
    34081 cttcttcagc ccgcttgcgg ttggtgatgt cttccaccga cagcaccatg ccgaccaggt
    34141 cgccctcgct gtcgaaattg ggcgtggccg aaacctcgga ttcaaaggag gagccgtccg
    34201 cccgcacggc gaccatctcg ccgcgccagg aaaccaggtc gttcaaaacg gcccgcacca
    34261 tctcgccggc gtgggtgtcg ttctgccaga gccggaccag gtcggtatga agcacgtcat
    34321 tctggtcatg aatgccccac tgccgcagaa aggacggatt cacataggtg aggcggccgt
    34381 ccacgtcggc gatggcgatg cccgtgccgg cattctggat ggcgttgaaa ctggttatca
    34441 ttttctcttc ggcctctttg cgcagcgaaa tgtcgcgcag gaagaagcac atcaggggat
    34501 cggcgaattt cagcaggttc acggcgattt ctgcggggaa gaaggtgccg tcccggcgga
    34561 tgcagtaggc ctggatcagg gcaaaacgct cgttctggag attgtcccga agagcggaga
    34621 gcagcgtttt ttcggccccg gccagaatat ccaccacgtt catctgcagc agttcatccc
    34681 ggtcgtggcc caggaagtcc agcgcgcgga tgttcacatc ggtgatcttt ccggaaagat
    34741 ccgcaatcag caccccgtca taaatgctct gcagcaggtg ctggtacttc gaatccatga
    34801 tctgggtgga tttcttggtg cggaccttga tgccggtcga ggttttgaca aacgacgtgg
    34861 accggtcccg ctgctgcacc aggtccttga gcgcagcagg ggaaatttca atccgcatcg
    34921 tgctggataa atttttctcc gttgccataa aacaaaaccc atccaccctc ttccggcccc
    34981 cccggccggg cagttactcc ttcttcggat aatagcacac cgtgctgttt agaacagcag
    35041 tttctgtgcc atattcggcg ggaaaggttt tttccctcag gatcctgcgc cggtgttggg
    35101 gacgaactcg acattttcga aaaaagcgcc gggcacgaag agcatcgggg aaacgggctc
    35161 attgacggcc aggctgctga tgcgcacggt ttccgtgagc tgcaggccca tgtcgctgaa
    35221 ctcgccccgg atcagcccgg caaagaacac gcccggcagg ggctcggaaa aagagctgta
    35281 tgcataacgg ccgatgcgtt catccatggc cgaagtcctg taaaattcca cctgaaccag
    35341 ccggctttcg ccgtccgcgc aaagcacaac gaaatttcgc gcggtctgat aaccggcccg
    35401 caccggaaac tccggtgtgc cgggaagggg ctgctcctcc acacccttca ggcggagcag
    35461 gtgatccatc gccgtgccgg gcaggcggcg cagttcaatg agcttgtcgg catccagttc
    35521 ggaaacgggc cgggagtagc cccggggttc gtcctgctcg tggatataga gcgctttgcc
    35581 gtccgaaacg attctgcgcc ggccggtgcc cagcgagggc atcacgtttt caatatggat
    35641 caggtcgggc ttctggcaga acacccggct cagcattctg gattttccct gcgcggactc
    35701 ggtgtcccgc cggacttcgc aactgaccgt ttcaactgcg tcataggcgg ccagcatcct
    35761 ctcggccagc gtgtccgcat gcaggtttcc cgccagggcc agcagcgcgg cggctgtaac
    35821 tgttttcttc atttgcgttc ctcgttaata atcaaccgca gaccgataac atattcatgc
    35881 gggttcggga acagcatttt cgccgccggg caggtccgcg ccgcaaaaga gccgacagaa
    35941 aacttccaat cattggaact ttcaggtaaa aaacttccaa tgattggaac tttttcccga
    36001 aaaacttcca atggttggaa ctttttgcgg gcgaacttcc agtgattgga aaaaagacgg
    36061 gcggacgcat ggcggaaaac gcatcgaatc ctgtaattgc ggcggcggac ctgaagaagg
    36121 tgttccgcga tttctggcgc cggccgaagg tccgcgcggt ggacggcatt tcatttgaag
    36181 tgagccgggg cgaggtgttc ggcctgctgg ggcccaacgg ttccggaaaa tccacaacca
    36241 tcaagatgtt gctcggcctg ctgtttccca cgagcggccg cctgcgcgtg ctgggggagt
    36301 cgccgcggca tgtgaagacc cgttcccgcg tggggtatct gccggagcat tcatatattt
    36361 atccgcatct gacgtccgag gagacccttg atttctacgg ccggctgttt gacctgagcc
    36421 gccgcgagcg ccgccgccgc atggagcagc ttctggagat gatcggcctg cagcatgcgc
    36481 gtcaccggat ggtgggcgaa ttctccaagg gcatggcgcg ccgcatcggc ctcgcgcagg
    36541 ccctgatcaa cgatcccgac gtggtggtgc tggacgagcc gacctcgggg ctggatccga
    36601 tcggatgccg gcaggtgaaa gatttgatcc tgacgctggc gcgccggggt aaaaccgtgc
    36661 tgatgtcgtc ccatctgctg gcggacgtgg aggatgtctg cgaccggatt gcgattctgt
    36721 acaacggtaa aatccaggcc atgggaccgg ttcgcgagct tctggaggag aaggggcgcc
    36781 gccggatcac gctccccgag ctttcgccgg aggcgatggc gcgtgtgctc gaactcctgc
    36841 ggaaggagtt cggagaggag ccggaactgg agcatccgcg gcgcgatctc gaacagttct
    36901 ttctcgatgt ggtggaaaag gcccgccgcg gcacgcaggc ttcgcccccg aagaaggaga
    36961 aactggccgg ctatctcgag ggcgaagatc tgctgcagaa gctggccgcg ccgtccacga
    37021 cggaaacgaa acgggaggag cctccgccgt ccgcgccccc gcccgcggcc cggacggacg
    37081 ccaggctgag ccatcttctc caaaacggtc cggacaatga atagggtgct gtccattgcg
    37141 gccgttacgc tgcgcaccgc ggtccgttca aggattgtgg ttttcatgat gctcctgctt
    37201 gtttttatcc tgacgggcct cccgtccgcc attcgcggag acggcacact gacgggatac
    37261 gtgcaggttc tgctgagcta cacgctcggg ttcgccggat tcattctgtc ggtggtggcc
    37321 gtctggaccg gatgcacggc ggtttcggcc gaaatcagga cacatcagat ggatctggtg
    37381 gtcacgaagc cggtgccggc cgttcagatc tggttcggaa aatggctggg cattctggcg
    37441 ttgagccttg cgctgctgct gttcacgttt gccgcagcct acggcggtct ccggtgggcg
    37501 atgattcacc gctccgccag tgaagtggat attcagaagc tgcgcgagca ggtgctggtc
    37561 gcgcgcgaaa aaatcgctcc gcgcgacgag gggctggaag agcaggccga aaacctctat
    37621 cgcctgcggc aggcggaagg ggccgtgccg gaaggagtgg cgccctcgac cgccagaagg
    37681 atcatcgccg acgacctgcg cctgcaggcc aattccgtgc cgccgggata cggccggcgc
    37741 tggatttttg atttcggctc gttcgtgaaa gaccggccgc tcttcctgcg ctacaccttc
    37801 accgcgtcgc gggccgagat tgaagggctc tggacggcgg gcgaaagcgg cagccgggat
    37861 caggcggtcc gacgcgtcac ggaggcctcc ggcggggttc gcacatggcg cattccctcc
    37921 gccgcggccc gcgacggttc gctcaccata gaattcggca acatccatga ggagcccgcc
    37981 acggtgattt tcccgccgga aaatccgccg gtccttctgc tttatcatga ttcttttgaa
    38041 atgaactttc tgagagtgtt ctgtattgcc tttatccgga tcgccgtgct ggcggccatt
    38101 ggactgacgg ccggcacact tttttcagag ccggttgcgc tctttttctc ggcatggatg
    38161 gtgctgctac tgaacatcgg ccgctatatc cgcgccatgg ccgccacgga tgttttcttt
    38221 cccgtccatg acggccccgc gcgcatcccg ggcctgctgg accggcttct gcatctgttt
    38281 ttcgcagcgc tggacggact ggtccggccg ctgcagagcc cggatgtgct gggcgcgatg
    38341 gcggccggag aatgggtctc ctggggcctt gtcgcggctg aatttcttgt gaaggtgctg
    38401 atcttctgcg gcctgctggc cgcgctgagt tcgtttgtgc tcaaccgccg ggaagtgggg
    38461 cttccggcat gaacaggctg gtgaatattc tgcttctgct tgcggcggtc ggcctgattc
    38521 ttctggccgg agcccggcat gaggaactcc ggcgtctcaa gatggaatat gagttcacgc
    38581 tggtggatcc cgttgagaac gccagtccgc tggttgcgtt cacaacagtg gcgctgggcg
    38641 gcttccgcgg cctcgcggcc gatgcgctgt ggctgcgggc gacgcgcctt caggacgaag
    38701 gccgctattt tgagctggtg cagctttcaa actggatcac gcagcttgaa ccgggcttcg
    38761 gcccggtgtg ggcttttcac gcgtggaatc tggcatacaa catcagtgtg aaatacagcg
    38821 aggcggagga tcgctggcgc tgggtgcaga gcggcattca gcttctgcgc gacaaggggc
    38881 ttgtctacaa tcccgcgagc gcggagctct accgcgaact ggcatggatc ttccaccaca
    38941 aagtgagcgg agttaccgat caggcgcact ggttctacaa gcagcagtgg gccgacgaaa
    39001 tggagcgcgc gctcggaggc gccacgcccg atttcgaggc gctcgaaacc cgcgcggacg
    39061 cgctggaccg ctatcgtctc gaacccgcct tcatgcagca ggtggaaaac acctacggac
    39121 cgctggactg gcggcttccg caggcgcacg cgatttactg ggccgcaaaa ggccggccgt
    39181 tcgcccgggg tttctacgag gtggcgctcg accggatcat tttcgattcc cttgcggagg
    39241 cgttccggcg cggaaagctt ttccgggacg atgaaagagg cgtgttcatc aactcgccga
    39301 acctggacct tcttccgaga gtgcaggcgg cgtacgccaa ggcgctggca gattatcccg
    39361 aagaggacag cattcggcgg ggtcacgaga atttcctgcg cgaggccatc atgattatct
    39421 acagccacaa ccgtgtccgg caggcgcggg agctgttcgc ggacctgaag gagaggtacg
    39481 gctcgcacat tgaggcggat acgctggagg agttcattcc agcggccacg gcggactttc
    39541 tggacaagcg cgcgccgcgg gaggcgcttc cgctgatcga gggcgccctc tatcagagcg
    39601 ccctgtggag tgcgctgggg gaggcggatt tggcggcggg ccaccgtcag ctggccaaac
    39661 tgtactggga tcacttcatg gaatcggccg tttcggagga atggatcgag cgcatggcgc
    39721 tgcctccgct ggaagaactg gccgccaaag ccgaaaagca ggcggcggac tacctgcagc
    39781 agatgccata gcgccggaac accgcctgtc cttgccggga catcaaacgg gtcggcgcgg
    39841 cctgttaaaa aatcgttttt tcttctggaa atgtcgggct gtcgcggata cgttacgtga
    39901 cctgagctcg attcacggcg cggtagccaa gtggtaaggc acgggtctgc aaaacctgta
    39961 tgcaccggtt caaatccggt ccgcgcctcc agctttgtcc gaggaaacag aaaatgaagg
    40021 ttccggaaca tctgctctac acccgaacgc atgaatggat atccattgag gacggccttg
    40081 ccaccgtggg catcaccggc tttgcggcac agcagcttga tgatgtggcg tatgtcgaac
    40141 tgccggccgc gggcgaccgc ttcatgcccg gggacgaggc cgcggtggtg gagtcggtga
    40201 aagctgcttc ggacatctat gcgcccgtgg cgggcgcggt ggaggaagtg aacggggagc
    40261 ttcatgatca tcccgaactc gtcagccagg atccctatgg cgaaggctgg ctcttcaaga
    40321 tccggcttgc ggttgaggac gaccacgacg gacttctcag cgaggaagaa taccgcaagc
    40381 tgattccgaa tgccggccag gaaactttcg atgacggcag tggccctgta ttttgacgag
    40441 cttcagccct ttgggccggt ctccgatctt cagtccaggc ttgtacaggc ccggcacaca
    40501 ggcgccattc cagacacggt tctttttctg gagcataaac ccgtcgtgac cctgggtcgt
    40561 cgcggccgcg accggttcct gaagctcccg tccgaagaat atgcccgccg gggcatcgag
    40621 ctgatgacct cgccgcgcgg cggagacgtg acctggcacg gcccgggcca gctggtcatg
    40681 tatcccgtta tcaaactcgg cgatcgcgag gcggacacgc acggctatct gcgcaacatg
    40741 gaggagatcg cgatccggac cgcggcggat ttcgacgtga aggcgttccg gcgcgaagga
    40801 aaaagcggcg catggaccga acagggcaag ctggcggcga tcggtttccg ggtgtcgcgc
    40861 tgggtgacca gtcacggcat gagtttcaac gtgtctcccg atctgtcggg atttgacacc
    40921 atcgtgccct gcgggctgga gggagaagct gtcgcgtcgc tgaaatccat cctgggcgaa
    40981 agatgtccgt cactgtcggc cgcgcgggat gccctgaaga cccgctttga agaggtctgc
    41041 gagcgtccgc tggaagtatt tgcctcgtgc gcgccgcccg cggagctcgc cggattcatg
    41101 aatttctgac aagccgcgcg gcaaaagcgc cgtcggttcc gctgtcgggc ggaaaaagca
    41161 gccggctttc gtcgagccgg aaatcaggat gcgcggccag ccagtgattc accatgaggg
    41221 tgttttcttc cggttcaagg ctgcaggtgc tgtagacgat gcgccctccg ggcttcacca
    41281 gttcggcctg atggtccaga atcatgcgct gggtgcggca gagttctcgc agccgctttt
    41341 cagaaaagcg ccattttgcg tcgggccgcc gtcggatcac tccggtgttg ctgcagggaa
    41401 catcgagaag aatgccgtca aaggcgtcgt cgcgcggcga catttttgca tcgccctgaa
    41461 gcacccggat gaaatctgcg ccggtgcggt tcatgttttc cttcaggcga tccaggcgct
    41521 cgggatacaa gtccatggcc gtcagtgatc ctgtccgccg catggccccg gcgatcatca
    41581 gcgccttccc gcccggcgcg gagcaggcgt ccaggatttt ttcgccgggc tgcgggttca
    41641 gcagggaaac cgcaagcgtg gtggcggggt cctgaacggt gaaatttccg tcatggtatc
    41701 cgggcagatc gcggacgggg catccggggg ggagcactag aaaatgctgc gggtcgaaag
    41761 gatgcggtac cgcggcgatc tttcttttct ccagtgcggc ccggatggaa tccatggccg
    41821 ccgggatgca aagaaccgtt cgtgccctcc ggttgttcca tttgcagagc cgcaccgcat
    41881 cctcccgacc ccattgatct gtccagcggc gaaaaaggtc ttcggggcag gagaggcgga
    41941 tctccggttt ctgccgggcc actccggaga gcagttcccg gcggcggcgg agcgcattgc
    42001 gcaggacggc attgacaaac cgcgcggctt tttcggcgcc ggcggcgcgg gcggcgtcca
    42061 cgcattcgct gaccacgaaa tagggtttgg cgctgttcat gtagagcagc cggtaaaggc
    42121 cggtcagcag cagcgcgcgc aggcgcaggg ccggcggccg cggggccagt tcgcggatca
    42181 tccattccag acttgcgcgc cagcggatcg caccgtagac cagttcgaga agaagtccgt
    42241 gctgctcccg cacggagtcg aggaggcggt ccgggaagag gcccttctcc agctgtgcct
    42301 cgacaatggc ggccgcctga atctgaatat tgccgtcggc tgattgcatg cgcagagatt
    42361 cggtcacccc ctgcttaaaa tcaaacgcaa agtgcggccg tttccggctt tcgcgcggtc
    42421 cggcaataat ctcatatagg caatattgcc gtattattaa aaaaatattt cctgcgcgga
    42481 agggagaaat aaagagcgca gttttgaaaa tgatctattt attacggcac acaaatgcca
    42541 taacatttgt gcaaacatta atatggaata aaaagcgggg tataaagaaa tgagcccaaa
    42601 aaagaacgag agcaagagcg aggcccggga gatgctgacg gacatctacg gggagaatgt
    42661 gttcagcctg aaggtcatgc gcgactatct ttcggatgcc gccttcaagt cgctttccgg
    42721 aaccattcgc agcggcgcaa cgatagaccc gagaattgcc gacgaggtgg ccgatgcaat
    42781 gaagatctgg gcgctggaac gcggggccac tcactacacg cactggttcc agcccctgac
    42841 cggcaccacc gccgaaaaac atgattcgtt cattgtcccg gacggcgagg gcggagccat
    42901 tgccaagttt tcgggcgaag agctgattca gggcgagccc gacgcctcca gttttccgtc
    42961 gggcggcctg cgggccacgt tcgaagcccg cggctatacc gcgtgggatc ccaccagtcc
    43021 ggcgttcatc aaggaggatg gtcaaagccg gacgctctgc attcccacgg tcttctgcgg
    43081 atatcacggc gaggcgctgg acaagaagac cccgctgctg cgctccatga aggcactgaa
    43141 cacgcaaatc tgcaggctgg caaaactgtt cggggtgaaa tcggacggct gccgggtgat
    43201 caccacgctt ggttccgagc aggaatattt cctgatcgac aaggaacttc acgaggcccg
    43261 gctggatctg ctgcagtgcg gacgcacact gttcggccgc tcgcccgcca agcatcagca
    43321 gctcgaagat cactacttcg gcgcgatcaa gagccgcgtg gtggcattca tgaccgatct
    43381 ggaccgcgag ctctggcgtc tcggagtgcc cgcgcgcacc cggcacaatg aggtctgtcc
    43441 cggacagttt gaaatcgcgc ccatgttcga ggagctgaat ctggccgtgg accacaacat
    43501 gctgacgatg gaaatcatcc gccgcgtggc cgaagagcac ggctttgcgt gcctgctcca
    43561 cgaaaagcct tttgcgggcg tgaacggctc cggcaagcac aacaactggt ccatctgcgg
    43621 tccggatggg aagaactggc tgagccccgg cgacgatccg catgagaatg ccaagttcct
    43681 gacggtgatc tgcgcgctga tcaaggcggt ggacacgcac gccgatctgc tgcgcgccgc
    43741 cgttgcctct ccgggcaacg atcaccgcct cggcgcaaac gaggcgcccc cggccatcat
    43801 ctccatcttc ctgggcgaac agctcacgga tattattgac cagatcgaaa agggcggcgc
    43861 aaaccgttcg cacaagggcg gcatgatcga gctgggcgtg gattccattc cccctctgcc
    43921 gcgcgggaac acggaccgca accggacatc gccctttgcg ttcaccggga acaaatttga
    43981 attccgcgcg gtgggatcgg agcagaatcc ttcggccgcg aacgtggtgc tgaacaccat
    44041 catggccgag gcacttgatg agatctgcac caagctggag aaggcgggcg cggaggaatt
    44101 caatgccgcc ctgcagagca tactctccga catcatccgg aagcacaagc gggtgctctt
    44161 caacggggac ggctatacgg acgaatgggt ggccgaagcc gagcggcgcg gcctcccgaa
    44221 cctgaaaaag acgcccgagg ccctggcttc ctaccgcacc gaaaaggcaa aggccctgtt
    44281 cagcaaatac cgcgtgctga gcgagcgcga actggcctcc cgctacgagg tgtacatcga
    44341 agcctatcat cgcgtggtca aaatagaagc gcgctgcgcg gtaaccatgg cccgcacgat
    44401 gatcgctccc gccgccattc gcgcgcagac cgaactggcg gggaacatca agtcggtcaa
    44461 agaggcgggc ggatctgccg cgggcgccca gtcgcttctc gaggaaatct gccggcatac
    44521 agaaatgatg ctctccgcca ttaagtcgct ggagagccgg atagccgaag acgatgtgtc
    44581 gggcatgatt tccgccactc tggcgctccg ggagcccgtg gatgagctgg agggactgct
    44641 tccggatgac gcatggccgc ttccgtccta tgccgaaatg ctgttcatga tgtaggctgg
    44701 ggaatcccat gcaaacgatc aggaaggaga gggacgacgt cccggttcga cagtgcagga
    44761 actgttttcc cgcgccgcag ggcctttacg atccgcagag ggaacacgat gcctgcggcg
    44821 tgggtttcct ggtgaacatt cagggagaga aatcgcaccg catcatcgaa cagggcatca
    44881 cggtgctcaa aaacctgctg catcgcggcg ccaccggcgc cgatcccgag accggtgacg
    44941 gggcggggct tctgttccag attcccgacg cgtttttcag gaaggcctgc ggggcgctgc
    45001 gcatggatct tccgcccgcg ggggaatacg gggttggcat gctgtttctt ccgcgtgatc
    45061 cggatcgccg cgccctgtgc cgcgccgcgg tcgagcgcgc cgcggaggac gaaggcctgc
    45121 gattcctcgg ctggcgtgat gttcccgtgg acagttccgt gctcggccgg aaggcgctgg
    45181 aagagatgcc ttttgtggcg cagtgttttg tttccggggc ctccggcgaa acgctggagc
    45241 ggaaactctg cgtaatgcgc aagcaggcgc agggaaaagc cggggcggtc cacgcgccgg
    45301 gtgacctttt ttacattccc agcctgtctt cgcgcaccat cgtgtacaag gggctgatgc
    45361 ttccgaaaca gattgcggcg ttttatcccg acctgaacga cgaagccctt gtatccgcgc
    45421 tggtcattgt gcatcagcgc tacagcacca acaccttccc ttcatgggcg ctggcgcagc
    45481 cgttccgcta tcttgcgcac aacggcgaga tcaacaccct tcagggcaac cggaactgga
    45541 tggcttcccg cgagaacaac ctgcgctcgg atgtgctggg cgatgacatc cgcaaggtcc
    45601 ttccggtgat tgaaccgggc gggagcgact ccgccgccgc ggacaatgtg ctggaactgc
    45661 tgtgccgggc gggccggtcg cttgatcatt cggtggcgat gatgattccg caggcctggg
    45721 gcacgaaata tcccattggg cccgatctga ggggtttctt cgagtaccat tcgggcctga
    45781 tggagccgtg ggacggcccg gcggccgtcg cgttttcgga cggccgtttc atcggcgcag
    45841 tgctcgaccg gaacggcctg cgtcccgcgc ggtacacgct cacccgggac gggttcatgg
    45901 tgttcgcatc cgaagcgggt gtgctggata ttcccccgga ggacgtgaag gaaaagggat
    45961 ccctcaggcc gggcgaaatg atcatggccg atgtgcagac cggccgcctg atgaaaaata
    46021 tcgagatcaa gcagggcctt gcgcgccggc agccgtatcg ccggtgggtt gaggagaacc
    46081 gcatcgacat tcatggtttc ttcagcgccg tcaaccccgt caccgccgac gaaaagaacc
    46141 tgaacttccg cgagaagctt ttcggctaca cgcgcgagga tttgacggtc attctggggc
    46201 cgatggcggc caccggccac gaacccgtcg gttcaatggg ttccgatcag ccgctcgccg
    46261 tgctgtccga gaaaccgcag cttttgtact ggtacttcaa gcagatcttc gcgcaggtca
    46321 ccaatccgcc gatcgatccg taccgcgagg agctggtgat gtcgctgatg acctttctgg
    46381 ggaacacgga cgacatcctc agcgaaagcc cccagcatgc gcggctggtg aagctgcagc
    46441 atccgattct ttcaaatgaa gacctccagc ggattcgcga acttccgcac gaggatttcc
    46501 gcagcgtgac gctgaagatg gacttctttt cgcgcggaag cgggcgggat ctggaggatg
    46561 cgctggactc actctgccgg caggccgaga cggccgtgcg gagcggggcc cggattgtga
    46621 ttctcagcga ccgggacctg gcgggggacc gcgcgccgat accggcgctg ctggccacgg
    46681 cggcagtcaa ccgccacctg atcgggcatg gcctgcgcac ccggacggga ctgctggttg
    46741 aaacaggcga agcgcgggag gtcatgcatc tggcgctgct gctgggctac ggggcttcgg
    46801 cggtcaattt gtacctcgcc tatgagatta ttgccgatct cgcggtgaag aagcagctcc
    46861 cggtgaatct gcctgtcacg cgcgccacta agaactactt cgtcgccctt cagaaaggcc
    46921 tgctgaagat catgtcgaaa atgggaattt cgaccctgcg cagctaccgc aatgcgcagg
    46981 tgtttgaagc ggttggcctg agcagtgagc tgatcagccg gtatttcacg ggcacacctt
    47041 cgcgcgtgga gggcatcggg ctggatggca tcgcgcgcga ggcccgggcg cgatatgatt
    47101 ttgcgcagga ccggccgctc tacggggacg tgctgaccag cgggggacag tacgccttcc
    47161 ggaaggatgg agagcggcac ctctggaccg cggacaccat caccctgctg cagcaggccg
    47221 cgcgggagaa cgaccgggag aaataccggc gctatgcgga gctcattaac aaccagacgc
    47281 agcggcaaag cacactgcgc ggcctgctcg ggttccgcgc gcagaatccg gttcccctgg
    47341 aggaggtcga gccggccggg gaaatcatga agcgctttgt caccggagcg atgtcgttcg
    47401 gatccatcag ccgggaggcg catgagacgc tggcggtggc gatgaaccgc atcgggggga
    47461 aaagcaacag cggcgagggc ggagaagacg ccgcgcgcta ccggccgctg ccgaacgggg
    47521 attcgcgatg cagtgcgatc aagcagattg ccagcgggcg tttcggcgtg acggccgaat
    47581 acctggtgaa cgcaaaagag ctgcaaatca agatcgcgca gggagcaaaa ccgggcgagg
    47641 gggggcagct tcccggacac aaggtgaacg cggaaatcgc gcgggtgcgc cactccacgc
    47701 cgggcgtcac gctgatttcc ccgccgccgc atcacgacat ctattccata gaggacatcg
    47761 cccagctgat ttatgacctt cactgcgcaa accgcgaggc gcgggtctcc gtcaaacttg
    47821 tctctgaaac cggagtgggc accgtggccg cgggtgtggc caaggccaag gcggacatga
    47881 tcctgatcag cggctatgac ggaggcacgg gcgcctcgcc gctgtcgtcc atcaagcatg
    47941 ccggtgtgag ctgggagctt ggactcagcg aggtccagca gacgctggtg ctgaacaatc
    48001 tccgcagtcg tgtgcgcctg caggtggacg gccagctcaa gaccggccgg gatgtcgtga
    48061 tcggggcgct gctgggagcg gaggaattcg ggtttgcgac cgcgccgctg gtggtctgcg
    48121 gctgcctgat gatgcgcgac tgccatttga acacctgtcc ggtcggcgtg gccactcagg
    48181 atccgcgcct gcgcaaatgc ttccggggga agccggagca tgtggtcaat ttcatgcgct
    48241 ttgtggccga agaggttcgc gaatacatgg cgctgctggg tgtgcgccgg cttgatgacc
    48301 tggtcggccg gacggatctt ctgcagatga atgaagccgt tgatttctgg aaggcccgtg
    48361 gaatagacct ttcgcgggta ctgtatcagg ctccgggccc ggaacaggac aggcgctgca
    48421 cgcagaagca ggagcacggc gtggaagatg ccctggacct cgagatcatg gatcgcattc
    48481 ggcctgccat cgaaaaagcc gagcgggtgg atttgactct gcccatccgc aacagccggc
    48541 gcaccgtggg cgccatgatt tcgtcggaga ttgcccgtcg ccgcggagga cagggcctgc
    48601 cggatgacac catcacgctg cggttttccg gttcggccgg gcagagtttc ggcgctttct
    48661 gttcgcgggg gatgaccctg attctggaag gggaggccaa tgattatgcg ggcaagggcc
    48721 tgtcgggcgc aaagatcatt attcgccctc cggccggctc ggcctttccc ccgtcggaga
    48781 gtgtaattgc cggcaacgtg cttctttacg gggcgacctc gggcgaactg tacctgaacg
    48841 gccgcgccgg cgagcggttc tgcatccgca acagcggcgc gattgccgtg gccgaagggg
    48901 caggtgatca cggatgcgaa tacatgacgg gcggccgcgt ggtcattctg ggtccagtcg
    48961 gtgtgaattt cggcgcgggc atgagcggag gcattgccta cgtgtatgac gaaacgggaa
    49021 cctttgacga aaactgcaat ctcgacatga ttgatcttga atccgtcagt tcggcggaag
    49081 acgagcagga actgaggggc atgatccaga agcatttcga ctgcaccggg agttcccgtg
    49141 cggcggaaat cctgcgcaac tgggattccg cgcgggcgct gttcgtgaaa gtgtttccga
    49201 tggaatacaa gaaggcgctg gggcggctca gccgcgagga tgcggaaacc gaacgcgcgg
    49261 aggtggtcaa tgactaagcg ctttggtttt ctggaccggc gcagggaccc gggctatcgt
    49321 ccggcggttg tgcgggttca ggattttgag ccggtggagc agacgcttac gccggaacag
    49381 atgagggagc aggcgggccg ctgcatggaa tgcggcacgc ccttttgtca cggccacggc
    49441 tgcccgctgg ccaatgtgat tccggaatgc aatgagctgg tttatcaggg acgctgggcg
    49501 gatgcgctgg agcttctgct gatcaccaat ccctttccgg agttcaccgg ccgcatttgt
    49561 cccgcgccgt gcgaaacatc gtgcattgcg ggcctgcacg gcgaacccgt caccattcgc
    49621 gaaatggagc gcgccgtaat ggactacggc tacgaaaacg ggctgctgtc ccggaagccg
    49681 cccgtgcgaa aaaatcagcg tgtggccgtt atcggctcgg gcccggccgg ccttgccgcg
    49741 gccgaaatgc tgaaccggga gggctatcgg gtgacggtgt atgatgacgc ggagaaaccc
    49801 ggcggcattc tgcgctacgg catccccggc ttcaagctgg acaaggcgct ggtggatcgg
    49861 agaaccggcc tgatgagaga ggccggaatc gtttttgaat gcggagtgcg cgtgggtgag
    49921 gatgtggcct tccggtatct gcgcgaccgt ttcgacgcgg tctgcctctg ctttggcgcc
    49981 cggaagccgc gcgatttgga ggttcccggc cgggaactgg ccggcgttca ttttgcgatg
    50041 gactatcttg tgcagcagaa cctgagagaa gacggtcatg cgatagaccc cggggcggag
    50101 attgccgcat acgggaaaaa agtcgtggtg atcggtggcg gcgacacggg atcggactgc
    50161 ctgggaaccg ccctgcgcca gggcgcggcg agcgtgcttc agattgaaat catgccgcag
    50221 ccgcccgcgg accgcgcacc ggagaatccg tggcccgaat ggccgcgcat tgtgcgcgca
    50281 accagcagcc acaaggaggg cgggacccgc cgctggtgcg tgaacacgaa ggcgtttctc
    50341 ggaaaccgcg ggcgcgtgtg cgggatgcgc tgcgtggaag tggagtggtc cggcgggaaa
    50401 tgttctgaaa aggagggatc agaattcgaa attcccgctg atcttgttct gctggccatg
    50461 ggttttaccg gtccggcgga aaaccggctg gccgcctcca ttggccttga actcgatgcg
    50521 cgcggaaaca tccggacaaa cgcggaccgg cagaccaccg tggatggtgt cttcgccgcc
    50581 ggcgatgcgg tcagcggcca gtcacttgtg gtgcgcgcaa ttgccgacgg aatgacagcc
    50641 gcccgcggca ttatgcgtta tcttgaagcc cgtccctgaa aaagagaagc cgtccggtat
    50701 tcgggggagg ggggcaccgg acggctccag ccgccgcgtt tccgcaacgg cgtttttaaa
    50761 ctatttatgg tgtttaacct aaagcctctt caaataaaaa gtcaatacca aattgctgtt
    50821 tatttttcag gaactactgg gcagcagcag gcccctcatc agcgggaact tccgcatcag
    50881 ccggctctgc gggcgcgggt tcaaccgccg cgggttctgc cggggccggt tctgtcgcgg
    50941 gggccccggc cacaatcatg ccgtcggaac caaccggcca gtcgggaccc cagtggtgag
    51001 tgaggctcca gtaggccttc tcgtcatctc caacgggttc tgtctggggc gcataggcta
    51061 gctgcatctg ccgggcgtcg cggccgaagc gttcaatgat tttcaaaacc gcattgttca
    51121 tcacgcggcc gtagctgctg ctcaggcgaa tgatgcccgc ttcacgctcg aaggtgctgg
    51181 cttttgagtt ctgcacaatc tcgtaggccc accagcactg gaccgtgggc tcggcccgcc
    51241 accggtcagg agtcttgact ccgttccatt tcacctgcag cgccagttcg gcggcggttt
    51301 tcaggtcctg cagaatggaa aacgcatagc gcgccgaccg ggcggagggg gtgaggaaga
    51361 tcacatcggt ctttccgtcc ttctgcccca gatagccctt catgatcacg gcctggatat
    51421 gccgcttgag ctgcggggga acgatgtgct gggggtcggg gaatacaaga gtgaaataga
    51481 ttggatcatc gaggctttcc acaagcggca tgatatcgcc ccgaaagatg gccgcaatca
    51541 gttcctgatg gggaccgatg gtttcctcct gggcaaaaag gaccatgtcg cgcccgagga
    51601 cgccaagcgc gatgttgccc gcatcccaca gatgagagtc caccattttc ttcatgtagc
    51661 catcgcggtg gaatccgatc tttcttgcat cgggcggaaa tccgcccatc acatgaacca
    51721 cgacatcgcg cggctgcccg tcatcgcctt tccggtgcgt caccatcatg gcggagactt
    51781 cgccgaggtt caaaacggga gtttcctcaa gccccttcgc cacaacggtt ctcagctgat
    51841 gaaaaagctc gggatcttcg ccgaagatgc ttttcagata atcagcgtcg aatgcatctg
    51901 atgcgggcac ttcgccaatg gtttcatctc cggccgtcag atagcgcagc tgcgcgcgca
    51961 tatcctttgc gacattccac acccggtggg cgcgaaaagc cagtgtgccg gccgctccag
    52021 tcagcaaccc caccgtcagc accgatcccc agaaaaattt tgatctcttg ctcatcccaa
    52081 ccccgctatg tccggttcaa gggcgtaatt cacctgctga aaaacatacc ccatcacggc
    52141 attctcggca acaggaaatc cggctatttg gccggagggg tgttctggcg tctctgtttg
    52201 aacacgctcg aaattattat tatgagtccc gcggctccaa tgacggccag gacagcaatg
    52261 gcgcccttcc ggttttcctc cttcgggctc cgcgccgccc ggcccacatg aatggaggcg
    52321 gaagatatct gccttggcgg agggggaatc tcgggccgtc cggcgtccac ccatgccgtg
    52381 taccagacgc tggacaggtc ggccgccgcc cggctgatct gccggaggaa cacatcctgc
    52441 gacttttccc acagctggaa gaagtagtac tgcgactcca cgttgtttct gcttgcccag
    52501 cgcgcctcgt tgtcggcctg gagaatattc gcgcagagcg gccaggcttc catgatccag
    52561 ccgttgacgc agttccacag gttggtgata tagaccgccg gttccggact cgtgacggtt
    52621 gcggccgagc cgattcgccg gggcatttga acctcccagc gcagatgaat gccggcgttg
    52681 cccgtctggt agccgtcata attcacggtg cagtgcaggg gctggtgggt gtcgcccaca
    52741 tagtgcccga tggcggccgc gatgcgggca gcctgatacc agtccttctc ccgcatggct
    52801 tcggtcagcc gctccatcgt atccatgatt gcccagggcg cttcgccctc ccgccgggcg
    52861 ccattcttca gcggggaaag atttcgcggg atttcaaacg gcggcgtccc gtaatgctca
    52921 aggtcaatga aatgccgaac aggttccgcg ggatcatccc tcttccagaa atccggatcc
    52981 gcgccgtgct cggcaaggag cggggcgtag gcgcgccatg cgctcatttc ctcggggaca
    53041 ttctgggcgg ccgcctcatt gatcaccgca tgaattgcgc cgccccagcc cgaaacggaa
    53101 aaggtcatgc agatgaagag gagcgggaac agaataagta ttcgatgttt ctgcatagat
    53161 atggttacca ttaaatccag atatttcaag ccggccaagt atgacaggga gaatgcgcgg
    53221 tatgaagcat tatttcgttt atattacatg cagcgacgcg gagcaggcaa agcgcatggc
    53281 tcttgcgctg gtggaagagg gtctggccgc ctgcgcgaac atcattccgg gcatgcgttc
    53341 agtgtaccgg tggaagggcg ccgtcgaaca ggcggaagaa gcggttctga ttgccaagac
    53401 gacggaggat atggctggtc cgctgactga ccgcgtcagg gatctgcaca gctatgaatg
    53461 tccctgcatc gagatgctgc ccgtcgcgga cgggaatccg gattttcttc gctggattga
    53521 ggattcggtt aataaatcct gatcaggcct cgcgcagggg gcgtccggcc tcatagagcg
    53581 tgatattttt ctggatttcg ccggcaagct ccgactggcc ctccttggcg gcctgcttgg
    53641 ccgcgctctg ggccacgccg atggcctcct tgaaattccc gttctcggcg caggccgccg
    53701 ccagcgtctg catgacgcgc acgtcgcggc ccttggaaag tttgaaggcc tgcttggcga
    53761 ttttcagggc ctgggccccg tcgcggatat ggctgtccac gcaggtggac atcacccacg
    53821 ccatggtgtt cagagcctcg acatcgctcg ggttttcctg cagccgcgca tgcgcctcct
    53881 gcagcagagc ttcgtgctgg acgccctcgc tgtccgttcc ctgaaggtcc agcgtgaatc
    53941 ccgcctcggt gatcttcttc agcacttcga ttttgtcgat ggccttgaga taggcctcct
    54001 tgggagtgat gactccgtcg cgaatcaggc ggatcagctc gtcattgaag acctgcatgc
    54061 cctcgctctt gccggtctgc atggcgctgg ggatgtggtg ggtcttgcct tcgcggatgt
    54121 tggaggcgac ggccgtgttg atcaccagca cctccatcac ggccacgcgg ccgccctgcc
    54181 ggcgcttgca gagtgtctgc gcgacgacgc ctttcagcga gtcggccagc atggtgcgga
    54241 tctggttctg ccggtcggga gggaatttgt caatgatgcg gtccacggtg ctggcggccg
    54301 tggtggtatg cagcgtgccg aacaccagat ggccggtttc ggcggtttcg atggcgattt
    54361 cgatggtctc caggtcgcgc atttctccga ccagcaccac atccggatcc tcgcgcagcg
    54421 cggcccgcag cgcattggag aagcttcgtg tgtgcttgtg cacctcgcgc tggttgatca
    54481 ggcaccgctt ggtcgggtgc acaaattcaa tcggatcctc aatggtgatg atgtgctccc
    54541 ggcggtcgtt gttgatcatg tcgatcatgg cggccagcgt ggttgatttt ccgcttccgg
    54601 tcggacccgt gaccaccaca agcccctttg aaagatagca gaaattccgc acggactgcg
    54661 gaaggcccag cttgtcggca ttgggaatca ccgacgggat gatgcgcatc acgccgccga
    54721 gtccgttgtg gtccatgaaa atattcacgc ggaaacggcc gaccccctcc agctcgtagg
    54781 cgaaatcggt gtcccatttc tcggcgaaca ccttcatgtt gtcttccggc acgaggggaa
    54841 gaagataagt ctcggtgtcc ttctgcgtca gttcggggtg gtcttcaatc cgctgcatgg
    54901 agccgtggat gcgaagatag ggtttcagtg aagtggtcag gtggaggtca cttgcttcat
    54961 gggcgaccat caggcgcagg agttcatcaa ttttgttggc cataatacat ccccggggtt
    55021 gtctatttca ccatctggct tcgaaggacc tgaagttcgt tttcaacgcg cgccagttcc
    55081 tctgtggttt ccttgagccg gcgggaaagc acctgcgcgc agatgtggtg aatgacggcg
    55141 aagcaggacc gctgttcctg atcattcagc atttcgagcc gctgggtgtg cacggaaagg
    55201 cagtagcagg gggtgacggc cttcaccgtc gccgctctcc gctcctggtt caggatcggg
    55261 tcctccccga agatttcccc atagccgcga atggaggcca ccggcacgcc ttttttcgtc
    55321 acggcgacct cgcccagcag cagaaaaaat atctggtggc caaactcgcc ctcctcaaca
    55381 atggtctccc ctgctgcgaa atcgcagagc tcgctggcgc ggaacagctc gtccaggtac
    55441 tttccccgga aatctttcag gtgcggaata tgctgcaggg tctgcttgta ggtctgcaaa
    55501 tcatccaacg gaagaaactg gcccatatgt catcctcgca tccggtaaaa cttgaaaact
    55561 cactgcaggc atagtagcgg cgtccggcgg tccggaaaag agaaaaaagg aaatcatccc
    55621 gccgaaaaca ggctgcgcac ttccgggctt acgcgtcgcg gccggtcggt ttcagcgtca
    55681 atggggcacc agagcgtgcg tgcgcgcgcc agcagccttc ggtcggccgc ccgcagaatc
    55741 tccgtcctgc gctcaaactg cagccccttt gccgagccca cccatgtccg cgcgatgacg
    55801 gtttcgcccg cgcgcgcagg cgccctgtag tcgatttcgt ggcgtatcac aacccatcgc
    55861 aggtgctccc gggcctctgc cggagcgatc tttgtccagt gggccacggc cgcatcctga
    55921 acccaccgga gatacaccgt gttgttcacg tggcccagct cgtcaatgtc ctccgcctga
    55981 accaggattt ccacttcaaa agggcttgcc gccggcatgt caaaggcctt tcttccaatc
    56041 aaaaaaacgc gcagccgcca ctttatcctc cgtgccggcc gcccggcaac atcaaactgt
    56101 gctgcggcct ccgccgggct gattcgccgg ataaaaaact ggaacctccc gcggccgacg
    56161 gctatgctct gtcttgtggt gtgcggcagc cggattgaaa aaggaggcgg cgattatgga
    56221 gcagctctct ctcctctcga aaatcagcct gtatgttatg ctcgcgttca tggggcttta
    56281 caccctgctt ctgctgggct ggcaggtgat gattctccgg ggccggtcct tcaaaaatcc
    56341 ggatggatca tccgatgact ggcatgagca gaaaaacttc tatggcatcg ccttcgccga
    56401 tgtgttcgtg gcctgtccgg tcaacgtcat cgggctggtt cttgttttca ttgcgccgcg
    56461 ctggggattc tatctgctgg cccaggccgc cttctggtgg gtttgggcca acgtgatgac
    56521 cacggcaacc agcattcggt tctacaatcc gcgccggagt cttggaacat ggcttctggg
    56581 ctacccgttc ggcatcctgc tgggcctggc ctacatcgcg tggaccattg tccactttga
    56641 tgtcatatat cctccatgat gccggcggga atatcccttg tcccgaattc tgcatcccgc
    56701 ccccgcttcg ggatattctc ttcttgatgg tgacaatctt cttgactgcc gaagagcaga
    56761 attctttaag gtcgcaattt gcgaccttaa agacgggccg ccgccaaaac agatcggatt
    56821 ccacgtaaag gaaaagcgcg ccgcttatac cactcggaaa accgggaagg ccatgaagtc
    56881 ctgaatccgg ccccggatgg atcctttgcg ttcttttgcg gtgaataaaa tattcgcgca
    56941 gagacgctgg ggcgcagaga taaagacgta aacgcgaact tcatgataaa attatcatct
    57001 tgaatcctga ctgcgttttg catgaccgtc gaaagtcctt ttattgaatc aggcattgat
    57061 gtagaaacat gccatgaaca cagcatcgtc acgaaaacca aggcccatga aggaatgcct
    57121 tatcaaaccg gtcgagccat tgattcttac catccgcggc cagcgcgtga ttctggacgc
    57181 cgatctggcg cagatttacg gggttgcaac caagcgcctg aatgagcagg tcaaacgtaa
    57241 cgccgaccgt ttcccggaag atttcatgtt tcaattgacg aaagaggaaa aagataaggt
    57301 ggtcgcaaat tgcgaccacc tcacccgact caaatattct ccgcaattac ccatgacctt
    57361 caccgagcac ggcgcaatga tggctgcgat ggtcttgaac agcccgaagg cggtggcgat
    57421 gagcgtgttt gtggtgcggg ccttcatcca gatgcgcgaa cggctggtcg ccaacgccga
    57481 tattttgaaa cggctggcgg agattgataa aacgctgctt gaacacaacg atgccctcgc
    57541 tttaatttgg gagcagatac agcctttgct cgctccgccg ccggaaccgc ccccaaaaca
    57601 gatcggattc cacgtcaagg aaaagcgcgc cgcctacact atccggaaga ccgg
//
LOCUS       DAOQWR010000002        53815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_654_length_53815_cov_5.350205, whole genome shotgun sequence.
ACCESSION   DAOQWR010000002 DAOQWR010000000
VERSION     DAOQWR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 53815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53815
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_53815_cov_5.350205"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PKK36_00255"
     CDS             complement(<1..1479)
                     /locus_tag="PKK36_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR93020.1"
                     /translation="MSFFFTGSGPALGYIGPGAGFAFVGSFFILLTALLLVAAAVLSW
                     PFRVLLSLLLRRGRKRRKTGVHRVVIVGLDGLDAKRTERMMEAGRLPQLRTLKEQGCF
                     KPLLSTCPPISPVAWSTFMTGVNPGKHNIYDFLNRSLRTYLPELSSSRIGSGRKSYAK
                     LLRRSRPFWQVAGEYGIFSTILRVPITFPPEKFNGVCLSGMCVPDLRGTQGSFTCFTD
                     SVQDSPLEGGMQVRVDFIGGRAKTFLPGPEFQPGAPLQVPLAIEREEDDAAVRIAVCG
                     QKFRLLRGRTSDWVSVRFRAGLFRSAYGICRFYFASDEKGFRLYVSPLNIDPARPALP
                     VSHPPFYSVYLARLIGPFATLGLAEDTWALNEGVIGDDTFVEQVYSIHEERKAMLMES
                     LKRVKKGIVACVFDASDRIQHMFTRDGGEGEAVVETMYEKMDELVGEVSARMRDGDLL
                     IVMSDHGFTSFRQGFNLNAWLLREGWLHLRPGAKEDKYLQAVD"
     gene            1574..2419
                     /locus_tag="PKK36_00260"
     CDS             1574..2419
                     /locus_tag="PKK36_00260"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR93021.1"
                     /translation="MNKDLTCFTVPPGKKDSSLLDFIADRMNLSKRKAKALLDARNVH
                     VNRRRIWMAQHRLKAGDIVEVQQTGLAGGHAQILFQDEDYLVADKPAGLLSNGPESLE
                     DRLRRELLVPGLRAVHRLDRGTTGCLIFAKHPEAFDAMIPLFREHGVIKVYKAIASGL
                     IQKKELTISEPLEGKPAETRLRVLAANQTASYISVKLVTGRTHQIRKHLAHIGHPLAG
                     ERTYGGGRSEDDALRAMPRQMLHAEKLRFTSPVTGAEVSCEAPLPQDFKSALRVLGLS
                     STPAR"
     gene            complement(2397..4046)
                     /locus_tag="PKK36_00265"
     CDS             complement(2397..4046)
                     /locus_tag="PKK36_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNR93022.1"
                     /translation="MSATGQPREHWNSSVGFILAAAGSAVGLGNIWKFPYITGENGGG
                     AFVIVYLLCIAVIGIPVMLCEICLGRHTQRNPVGAFKLLNPPTSRLAHFIGSMIILAG
                     IILLCMRNWGWGLMMIILGFSIFKFRWTVVGAMGVIAGFVILSYYSVVAGWTIGYFIE
                     AVLHRLDFSNVTAAGDHFGSFIGNPDLSIGCHFLFMFSCVFIVYKGVKSGIERASKIL
                     MPVLFLLLVLLIIRGLTLPGAMKGVQFYLSPDFSKITASSVLIALGHAFFSLSLGMGA
                     IITYGSYVQREQNLFMSTLSIVALDTLVALMAGLAMFPAVFAMGFSPDVGPGLVFQVL
                     PTVFNQIPLGMLWAALFFLLLFVAALTSGISLLEVVTAYFVDERNWTRKQASIVFGLM
                     IFMLGALCALSVGDWNSLASVHAALLKAFGHTKNSFFELMDYLSSSWMLPLGGLFISL
                     FVGWIWGVQKAVDEIRHGSHNFADVHLISLLAGLKDDPSHNSNVHVITLAALWGIFIR
                     FISPVAVMIAFLNIIGWVDFSPKEPAEAPPAVEAVSEQGSN"
     gene            complement(4095..4322)
                     /gene="infA"
                     /locus_tag="PKK36_00270"
     CDS             complement(4095..4322)
                     /gene="infA"
                     /locus_tag="PKK36_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNR93023.1"
                     /translation="MAGGKEDLIQVEGEVVKVLPATMYRVKLANGHEILAHISGKMRK
                     HFIRITTGDKVTVEISPYDLKKGRITFRHKD"
     gene            complement(4436..5692)
                     /locus_tag="PKK36_00275"
     CDS             complement(4436..5692)
                     /locus_tag="PKK36_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93024.1"
                     /translation="MKKQLQILSILLAALFVCATSRGQGVFSLDGFAVDTLESESPFA
                     ERDAYFEAEIPASYEDFEKYRKAEAAAGQALAQHRDLLAAAEHSISQGDWETARRNIE
                     TVLQAAPDNTDLLKKASLLCALAGEYNAADRYFERYVRLKPDDVAHVAAWAAVAVRQR
                     AWPKAEALLNRAFELSPRNLSAHFQAVVLRIATGRTKPLRNYNWHHLTLAEMYILANW
                     MEADRQAYEKILGEKGFDQLISETLGPVPVSALSEVRLAAQRAYAAKRRNQWQPFRDI
                     LLRLQDLGAQSYWVPLHLSEAHYALGSAAEALNYLQLVQQQHPQVVDAWRNAAFVRIK
                     TGDFEGAIADARRARRLAPNDPDVEFTLISALAGARRISELWPLLEQWARGNPRRFRE
                     AMRSEDPLLQIIREDPRFGALQRSLQ"
     gene            complement(5694..6404)
                     /locus_tag="PKK36_00280"
     CDS             complement(5694..6404)
                     /locus_tag="PKK36_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNR93025.1"
                     /translation="MNQVQEMELQAVIFDFDGVIVDTEPLHWRAFSEALRDDGLAFTW
                     EEYADYYIGFDDRDAFREVFQRAGKPLTSARLASLIRRKADAFQHLISTEPPEPYPGV
                     LDRLREISGQLPLGLCSGALRGDIEPVLERFELGSLFDAIVTAEDVQASKPNPACYRR
                     VLERLNAGRPSAIPPAGCLAVEDTTAGIASAQGAGMRVLAVSNSHPPPALASADRVMD
                     SLVSVSLDDLRIWFAGLK"
     gene            6435..7850
                     /locus_tag="PKK36_00285"
     CDS             6435..7850
                     /locus_tag="PKK36_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNR93026.1"
                     /translation="MNKDNSSLNEASRQITERMQAVRMPQLAIDTFLHHYAKLRAGAR
                     MEIPESDIRPVTDLPSSLDLGQHEPAGRSLMGKTVIIKLNGGLGTSMGLDKAKSLLPV
                     KNGMSFLDIIACQVSALRRKLGFSIPLVLMNSFSTEEDSLAALHKHKEFISGSPDIPL
                     SFLQHQVPKISKATGLPVTFPADPEMEWCPPGHGDIYTALVTSGVLENLLSRGIEYAF
                     VSNADNLGATLDFSILGYFASGGSPFMMEVTERTAADRKGGHLAQGRSGLLLREIAQC
                     PAAELDAFQNIAVHRYFNTNNLWINLRRLDAALRASQGILDLPLIINQKTVDPCDKNS
                     EAVYQLETAMGSAISAFEGAAAVCVPRTRFAPVKTTNDLLALWSDIYDLTQEYHVVVS
                     PRRTLGTIDISLDPSHYKLMKDFDERFRCGAPSLIDCESLTITGDIYFEDHIRLQGRL
                     LLKNRGRKGVCIPAGTTICGR"
     gene            complement(7914..9083)
                     /gene="metK"
                     /locus_tag="PKK36_00290"
     CDS             complement(7914..9083)
                     /gene="metK"
                     /locus_tag="PKK36_00290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNR93027.1"
                     /translation="MSKRCLFTSESVTEGHPDKVCDAISDAILDAALRQDKNSRVACE
                     SLVKTGMAIIAGEITTTASINYADVARGTIRRIGYTDSSIGFDGNTCAVLVAVEKQSP
                     DISQGVTAGQGLFKEQGAGDQGMMFGYACDETRELMPAPIMYAHRLCRELSRTRKSGR
                     LPFLRPDGKSQVTVVYENGRPVAVDTVVVSTQHDPDITHKKLSRAIIDEVIMKTIPKK
                     MLSRNTRCLVNPTGRFVIGGPQGDCGVTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMARYVAKNVVAAKLAARCEVQLAYAIGFAEPVSVLVDTFGTGRVPEEKIEKAVR
                     RVFGLKPAQIIRELNLLRPIFEATAAYGHFGRTSGLENFTWERTDKVKALLNALK"
     gene            complement(9129..10904)
                     /gene="ptsP"
                     /locus_tag="PKK36_00295"
     CDS             complement(9129..10904)
                     /gene="ptsP"
                     /locus_tag="PKK36_00295"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNR93028.1"
                     /translation="MPDPAAHEELVLKGIGVSPGVAVGEAYLIAPEVLRLVEHDIREQ
                     DIPGEIARFEQALIATRQQIHEIQKQVNKALGERKASIFDAHLMVVDDHSFVEKVVAG
                     LSEQKKNVEAVVYRVTEEFADVLSRVQDDYLRERAADVRDVARRILRNLSGHKLSDLA
                     GLQESCIIVASDLAPSDTATLNRDKTIGFATDLGSHTSHTAIMARALEIPAVVGLHDA
                     STRVPAGAVVLVDGTRGLLIVNPTAQRLTYYGKLAQEQRHIRSELAALQGESAETLDG
                     YRVILSANIELPSDSDAVIRHGAAGVGLFRTEFLYLSGDHLPTEDEQVEAYSSVATRT
                     APHPVIIRTLDLGGDKFVSHINTPLEVNPFMGWRAIRFCLAQQDIFKTQLRAVLRASR
                     HENIRLMYPMISNVDEVLQANEVLEKAKQELRAEGKPFNENIEVGIMIEIPSAALAAD
                     ILAPHVDFFSLGTNDLVQYTLAVDRVNERVAYLYEPAHPAIIRLIKHTIDTGREHGIW
                     TGVCGEMAANLLIVPLLIGLGADELSVSPVMVPMVKRIIRCLKYSQALELGEAALQSR
                     SASEILAQCRKLVEQIAPELLDFVV"
     gene            complement(10911..11183)
                     /locus_tag="PKK36_00300"
     CDS             complement(10911..11183)
                     /locus_tag="PKK36_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNR93029.1"
                     /translation="MSEHVRKVEIVNRLGIHARPASLFVKKANEFSADITVEKDGTRV
                     CAKSIMGLLTLEGYMGSVLILRATGADAPQALDALEELVKNKFFED"
     gene            complement(11204..12142)
                     /gene="hprK"
                     /locus_tag="PKK36_00305"
     CDS             complement(11204..12142)
                     /gene="hprK"
                     /locus_tag="PKK36_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNR93030.1"
                     /translation="MPVSVGQFYEAGKESLQMELEFGGEYFPRPIPESAINRPGLALT
                     GFFRHFSYRRIQVIGLAEFDYLRSLTAALRLERIREVCRRDIPCMVLTRNRHAIPELM
                     SAAQEYQIPVMRSPMITSRFINAATIIMENLAAPQIRVQGTMVDIMGIGVLLQGPPGL
                     GKSETALALIERGHSLVADDVTVLRRDSEGFVIGSAVQVTRYHMEIRGLGIIHVPSLF
                     GVASMRSNMRLDLVIQMQPWDPAADMDRTGLNSETMTFMDVSVPLIRLPVAPGRDMAQ
                     VIEVAALNQKLKRMGHDAAKELDQKLVNVLTEKSRM"
     gene            complement(12170..12514)
                     /gene="raiA"
                     /locus_tag="PKK36_00310"
     CDS             complement(12170..12514)
                     /gene="raiA"
                     /locus_tag="PKK36_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNR93031.1"
                     /translation="MQITVTGRHVDITSAIREYAEDRVNDAMALFPRVESVHVILSVE
                     KYRHRAEIVVQGANHIRIDAHDESDDMYASIDSAIEKAMRQLRKLRDKVQDHKGAEPL
                     AEAEVKVSKKEA"
     gene            complement(12537..13274)
                     /gene="lptB"
                     /locus_tag="PKK36_00315"
     CDS             complement(12537..13274)
                     /gene="lptB"
                     /locus_tag="PKK36_00315"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNR93032.1"
                     /translation="MVTDSTYLVSTEELEKVYGGRRVVSGVNINVRPGEIVGLLGPNG
                     AGKTTSFYMIMGLIRPTAGRVHFAGREVTRTPMYRRARMGMGYLSQEPSIFRRLTVEE
                     NVMAILETLPLSKQERRDRLEFLLNELKIAYLAKQRAYTLSGGERRRLEITRALVTSP
                     TLILLDEPFSGVDPLAVYDVQQIIMELKEKGLGILITDHNVRETLAVVDRAYLLCDGK
                     VLREGTREFLINDKVSRDLYLGPRFSM"
     gene            complement(13276..13773)
                     /locus_tag="PKK36_00320"
     CDS             complement(13276..13773)
                     /locus_tag="PKK36_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNR93033.1"
                     /translation="MSRRRTLLILAAGIAAACAPAVFAQGGKKEVTEITSERLTFDYN
                     EHYALFEENVVVVDPEMKLKADRMTVHFAEDNEVKSIIARGNVIIEQEDRTATAGRAV
                     YDVESGKIQLDEKPRVTRGNDVLEGTTISFWRDLNKMVCEPRARLVIYPQSGKTSRAK
                     TVSGE"
     gene            complement(13770..14201)
                     /gene="lptC"
                     /locus_tag="PKK36_00325"
     CDS             complement(13770..14201)
                     /gene="lptC"
                     /locus_tag="PKK36_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNR93034.1"
                     /translation="MKKGWNLLLMFVFCAAAAAQDPGVQSLAGFELPEYDADGKIVRH
                     IFGDMAEMVPGGLIRISNLRVDIYNNEQVEMKVIAPRCELNQQTKQAVSDGPVRIIRD
                     NMVVTGRGFDFSNEKERIQIMHDTKVVLKGFRSRVNPGDKK"
     gene            complement(14198..14731)
                     /locus_tag="PKK36_00330"
     CDS             complement(14198..14731)
                     /locus_tag="PKK36_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNR93035.1"
                     /translation="MPSSVEKKAAAIEAIIFDVDGVLTSGAIVYGPEGEWKIFNVQDG
                     HGFKMAARAGLKLGVLTGRSSAVVDRRMKELGVEFYEQGAMNKGEALPRLLRKMKVNA
                     GQTCYVGDDVIDLPPMRLAGLSVAVANAVDEVKAAADAVTARKGGEGAAREVIELVMR
                     ARGLWEEAMKRYTEDAE"
     gene            complement(14715..15539)
                     /gene="kdsA"
                     /locus_tag="PKK36_00335"
     CDS             complement(14715..15539)
                     /gene="kdsA"
                     /locus_tag="PKK36_00335"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNR93036.1"
                     /translation="MTKTKTVRVGQVGVGGGGPLVLMAGLCVIESRAICLDLARRLAK
                     LAEKEKIPLIFKASYDKANRTSGDAFRGPGMVKGLEILREVKDRFGLPILVDVHSETE
                     VPHAAKVADVLQLPAFLCRQTDLVLALGESGRPVNIKKGQFLAPWDIVNVIRKVESTG
                     NRKIILTERGASFGYNNLVADMRSLLIMRETGYPVVFDATHSVQKPGGAGGRTGGDGQ
                     FAPALARAAVATGCDGVFLETHVHPEKALSDRDNAIRFAGIAKLWKQLRGIHAVVS"
     gene            complement(15768..17111)
                     /locus_tag="PKK36_00340"
     CDS             complement(15768..17111)
                     /locus_tag="PKK36_00340"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNR93037.1"
                     /translation="MIQVQRIHALNSIPAGGGEYVLYWMQQAQRAEFNHALEHAACQA
                     NALQQPLVVCFALTSYPEANLRHYRFMLEGLRDVRGQLAKRGIPFCVRRGAPADVVQE
                     LARKASLLVADVGYTRIQKQWRRDAAGRAACRMEAVETDVIVPVQEVSDHEEFGARTI
                     RPKIHRILPLYLEPLRKVPVKYPFSGKPPASLSLEDIDAVCAGLKTDASVKPSSRFAG
                     GSHAARKQLSKFIAQHLDEYHENSSDPVLDACSHMSPYLHFGQISPLDMALQISGSDA
                     PRAAKDACLEQLIVRRELSMNFCWYNPEYDKYDSLPAWALKTMGEHAKDRREHVYTRE
                     QWEAAATHDPYWNAAQRELAKTGFMHNYMRMYWGKKILEWSRTPEDAFETALHLNNKY
                     ELDGRDPNGFAGVSWCFGRHDRAWTERPVFGKIRCMNANGLKRKFDIEAYVERWS"
     gene            complement(17114..18637)
                     /gene="crtI"
                     /locus_tag="PKK36_00345"
     CDS             complement(17114..18637)
                     /gene="crtI"
                     /locus_tag="PKK36_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNR93038.1"
                     /translation="MAEQAEKKHVVVIGAGPGGLTAAMILAHRGFRVTVCEAQPHVGG
                     RNGAIRLGPYTFDIGPTFLMLKDILDEVFREAGGDADKAMDMRRLEPMYRLQYQDKYI
                     ETSTDHEKMKSEIARVFPGHENRYDKFFKQEQTRFRYLFPCLQKSYHRFRTMFAPDMI
                     KALPYIAAGKSLFDVMYGIFGNRDLALTFTFQAKYLGMSPWECPGLFSIIPYIEHGFG
                     IYHPIGGLSRISETMADQARANGAEIRLSAPVEKIIVKDRRAKGVRLAGGEVIEADDV
                     VINADFGYAAQNLFEPGVLRKWTPQKLQKMRLSCSIFMMYLGVDKSYDLPHHTVFFAK
                     DYRRNVEDVFHLRDPGTDISFYVRNASLTDPTLAPPGHSALYVLVPVANLRAPVDWAA
                     KRDSFREVVLKNIEERTPMKDIRSHIREEKIIGPREWRDDLNVYEGATFNLAHNLTQM
                     LYFRPRNKFEEVGNCYLVGGGTHPGSGLPTIYESGRIAANLIARSHQVPFVSGNLEV"
     gene            complement(18671..20050)
                     /locus_tag="PKK36_00350"
     CDS             complement(18671..20050)
                     /locus_tag="PKK36_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNR93039.1"
                     /translation="MTESFCDLKMREYFAAGRTRDLAFRLQSLSRLARAVEQSEAEIL
                     DALKKDMGKPAIEAYASEPGFVLRDIRYTMKHLRRWARPRRESVPPMLWPGRARLIPE
                     PRGVVLIMGPWNYPFQLVLSPLVAAVAAGNCAVIKPSEYAPHTSAVIRHVLSAAFPDD
                     HVRVVEGDYTAAETLLQNRFDYIFFTGGTETGRRVMAAAARHLTPVTLELGGKSPAIV
                     CADADIPVAARRIARGKFMNAGQTCVAPDHVWVDRSAAGGFVEEIAKAVTEFYGPDPE
                     TSPDYGRIVSAKHVQRLARYLEGQTIVHGGQHNSASKYFAPAILLNPRLDAPVMREEI
                     FGPVLPVIEYSSLDTAVAEIQKMPSPLALYVFSSSNELAEDLLARIPSGGACVNDTIS
                     HLLVEGLPFGGIGESGIGAYHGRAGFDAFTHWRGTVRRGARPDMPQAYPPYGMTLKTF
                     KKIYRWLTG"
     gene            20164..20925
                     /locus_tag="PKK36_00355"
     CDS             20164..20925
                     /locus_tag="PKK36_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93040.1"
                     /translation="MTFAAAMLAALLCPLFAPARTPAHVLQGMALLEEGMSRWDPVRL
                     EQALEHFRRSTASNPEQFEPWYLKGLAEFHLMLVHESRAAEADKALTAKLRKDAEHSM
                     DRTRALKQDFGEASALKAVLMGMAIAEHPATAIWRGPRLQHLQKEALALDPENPRIWY
                     LMGMSRHFAPRPFGSRKKAREYLLRAAELFEHDQPGRRVFRYPAWGHAHCLMFLGEIS
                     REENNNREAAEFYREALAVNPHLDGIRKLLKEIQK"
     gene            20922..22409
                     /gene="crtI"
                     /locus_tag="PKK36_00360"
     CDS             20922..22409
                     /gene="crtI"
                     /locus_tag="PKK36_00360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNR93041.1"
                     /translation="MNRHKKVAVIGSGLGGISAAISLAQEGYPVSIYEKNDRIGGKLN
                     VLSKQGYTFDMGPSILTLPHIFERLFTRSGRKMSDYFPIRPLTPHWRNFFDDGTVVNL
                     FMEKDRMAAEAEQLEPGAAKQVEAFLNYAAEQYDLVEKGYFEHGLDTIRDFMKFYTGS
                     TFFKFDLWHTMHQGVAKRISNRYLQDIFDFFIKYVGSSAYDAPGFMNCLPVIQFRYDL
                     WYVDGGLYGIARGLGRLMDELGIRVHLNSEVTAIRKSGNRVTGLTLADGTAVDAGIIV
                     SNMEVIPACQRLLHEDAAFMKKLERFEPACSGLVLDLGLDCKYDQLAHHNFFFSSNQK
                     KHFKSVFRDKVLPHNPTIYLVAASRTDPTVAPPGCECLKILPHIPYISDSNPLSQEDY
                     LQFKEHIIDRLESMGLKDLRKHTVFEHVWTPLDIQRQYYSNKGSIYGVVSSMTKNLAF
                     KAPKQSTRYDNLFFTGGSVNPGGGMPMVVLCGQNVCKKIIEWDRN"
     gene            22394..23485
                     /locus_tag="PKK36_00365"
     CDS             22394..23485
                     /locus_tag="PKK36_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93042.1"
                     /translation="MGPELTLWLLDLGIWFAGILMLWRIPLCRKRPSSAPAPSVIIPA
                     RDEERNLPRLLESLKRQIPGPLEIIVVDDHSTDRTAEIARQAGARVTGSAPLPAGWTG
                     KTWACHQGAQAAQSKILIFMDADTWLEDGGLAGITSTWEEKKGALSVIPFHFTRKPYE
                     WFSVFFNVIMAASMNAFSIWSRTDRSEGLYGQFLMVSKEDYFKIGGHESVRQHILENL
                     HLAPLFRAEGIPVRCVGGERSLGIRMYPDGFASLAGGWRKAFGSGAACTPRPLLLLMV
                     AWLAAAAGTALMLLLAPLRTAGTGIAWLLLYLLFVIQMRWHFRRIGNFPLAAALFYPV
                     FLFFFFWISALPQQANWKGRTVGAAGNES"
     gene            23482..23961
                     /locus_tag="PKK36_00370"
     CDS             23482..23961
                     /locus_tag="PKK36_00370"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HNR93043.1"
                     /translation="MIIHLPPFWIAGLNVIACLVLQTGIAWGVTQIRASRFRPDAWLY
                     RKREWERNGHIYEDLFLIRKWKTLLPDGAAWFKKGFAKKRLNERSAEYYRRFGMETCR
                     GELAHWLQIACAPLFFLWNPAWAGWAVASFLLLANFPCILAQRYNRIRLASSRMCRR"
     gene            24045..24821
                     /locus_tag="PKK36_00375"
     CDS             24045..24821
                     /locus_tag="PKK36_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93044.1"
                     /translation="MLFAAVAVYCAYLFMPRPAPKNVPRLAAGMEIFRNGQSSRDAAV
                     MEKALRLFEDTCMKEPGSYEAWHMKSLTELHLLLHSRDVRDTEGEAVFSRAAEHSAMK
                     VTALQSTFPEAYAMLAAVTALRPPQRGQTAAVRRYEKLALMLGGNNPRVHYLLGLRYY
                     LSRGRLRNPDRAEELFLKAAGLFEREPPGARAFNFPSWGHAASLEHLGDIHEERGDVQ
                     AAAGYYRRALAVNPFLEPVRRSLQDLKDARPGTAPRGKSP"
     gene            complement(24818..25774)
                     /locus_tag="PKK36_00380"
     CDS             complement(24818..25774)
                     /locus_tag="PKK36_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNR93045.1"
                     /translation="MKTLRDIGECEAIRRLAGKLFPAVAEVVGIGDDAAVMHDEGFNA
                     DLVLTSDPVIEGTHFTIDAPPKGIGNKAVGRVLSDIAAMGAEPLWLLINIVAPPEMPF
                     DKLQQIYAGASALALKFGVAIIGGDVASGATLALHVFGVGKLPRGTAVCRGGASEGDI
                     LYVTGELGGSIRKKHLFFEPRVAEGIWLRENRWATAMIDLSDGLATDLRHLVEMSRAG
                     AELEPDALPVSRAAREMNDGVSPLDHALTDGEDFELLFTVAPGNADSFEQAWRAEFEL
                     SCARIGRITGRTGSIVCRTERGGMLLTGKRGYEHYSGRGSRA"
     gene            complement(25771..26829)
                     /gene="mtnA"
                     /locus_tag="PKK36_00385"
     CDS             complement(25771..26829)
                     /gene="mtnA"
                     /locus_tag="PKK36_00385"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNR93046.1"
                     /translation="MKVLSIEWISESAEGMMPGFIRMIDQTRLPEELAYLNTTDIEIV
                     WEAIKMLRVRGAPAIGIAAAMGVAAALQGSEASGERLLAEAEKHADYLATSRPTAVNL
                     FQALERMKSVARASASLPPAAFKERLVREAVAIRDEDAAMCRAIGEHGLKLIRDGQTV
                     LTHCNAGALAVSEYGSALAPIYAAQAHGMRVRVFADETRPLLQGARLTAWELMQAGID
                     VTLICDNTAAVVMKEGKIDMVMVGSDRIAANGDAANKIGTYGVARLARAHGIPVYFLA
                     PTTTIDMSLPSGDCIPIEERSAEEVTCGFGRRTAPEGVKVYAPAFDVTPAEYITAIVT
                     EKGAAYPPFGETLREWMK"
     gene            complement(26832..27746)
                     /locus_tag="PKK36_00390"
     CDS             complement(26832..27746)
                     /locus_tag="PKK36_00390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR93047.1"
                     /translation="MGCFLMLFLAEIPLELNPEALAQLPHAWMAGIFYFFLLLAGLGA
                     DALLLSNFRGASFSWAACAERLKARAWPSEDFRRLVLWLVFLHLAGMMMVQSLYAHDA
                     VSGDGSPMIVLVQSLIFHWAGLALAAAMLLQRGATGKTAFGLDWPGAARSAARGFYYY
                     LASLPVLLTGAVLAEFVLVRLGLEPQLQDIAGIVAGAHVWWIRIYLILLAVVLAPVFE
                     EVLFRGIALPWLARRTGMPAAVVLVSLVFAAMHGYLFQTIPLFLIAAAFSLAYIRTGN
                     LLVPVVMHAVFNGVNTAMLVVLQSGAGK"
     gene            complement(27758..28714)
                     /locus_tag="PKK36_00395"
     CDS             complement(27758..28714)
                     /locus_tag="PKK36_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93048.1"
                     /translation="MDVSVVVPVYNEEECVDALCTKLHESLSNLGRSYEIILVDDGSR
                     DGTWARLKEWTGKIPNLRIIRFRRNFGQTAAMSAGFHKSRGRIVITLDADLQNDPADI
                     PKLLENMDDNVDVVSGWRKDRKDPFINRRLPSMLANSLISRITGVALHDYGCTLKAYR
                     REVIEKVHLYGEMHRFIPALASWVGGDIKEVVVNHHARQFGKSKYGISRTFRVVLDLI
                     TVKFLLRYSMGPMQIFGKIGGLFAVPALLMMAVVAADRIFGWNDPYLLKRPFWLLTPL
                     MLLTFCIQFIGLGLLAEMQIRTYHESQDKPIYVIREAAESAV"
     gene            28895..30040
                     /locus_tag="PKK36_00400"
     CDS             28895..30040
                     /locus_tag="PKK36_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNR93049.1"
                     /translation="MNRKSADQTRRCTFRISTETPLPAGEQVFISGNDPALGDWAPDG
                     LPLTRVQDCLWTGTAVLPAGRTIEYKITRGSWQAEEALPSGAFSPNKTLRPGADSVIE
                     NKVLRWRDSHLPPAPQITGNYEIHRNVKSKFLAEPRDVIVWLPPSWSKKGAASYPVLY
                     MHDGQQVFDPNTSTWNHALDVDDYCTELMKAGEMQEIIVVAVYSTGDRSVEYDPAAKG
                     GDYAKFIVEELKPFMDKTYRTKPGRESTAVAGASRGGDISFHLAWTRPDVFFGAACLS
                     PAFRFEGREPCLDAVRNALTVPDIKCYLYCGSGDPLERELMPGMRDMARQLRLAGYKD
                     GVNLLVEEDEPAAHNEESWSRHTPSWLKFLFGIQNPKPARKARKNRK"
     gene            complement(30045..30509)
                     /locus_tag="PKK36_00405"
     CDS             complement(30045..30509)
                     /locus_tag="PKK36_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR93050.1"
                     /translation="MQKLVDKYKLLPHPEGGYYREVFRSGLKVSSAAAGAERDAATHI
                     YYLLTRGDISRFHRVLHDEIWNFYEGDPLRLIKYDGETIREERISGAGDGFVCIMGAG
                     IYQAAETSGEFSLIGCTVAPGFDFRDFSFLSDDKSASERIKTRHPEYARFIG"
     gene            30635..31870
                     /locus_tag="PKK36_00410"
     CDS             30635..31870
                     /locus_tag="PKK36_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNR93051.1"
                     /translation="MNFYLVFIVGILVAHYLLDLLADVLNVRNCSEQLPEEFRDVYDA
                     GKYARSQQYLKDHTRMDTVVNSAHTLIILAFILLGGFRLVELAARKAGWDMIGTGLLF
                     GGILMILSQLLHLPSSIYSTFVLEAKYDFNKTTPKTFVLDIVKSLALTVIIGAPVFAL
                     ILWFFDAAGEWAWLWCWLAVMLIQALLMFIAPVVIMPLFNKFEPLEDGELKTAIENYA
                     RGQNFKMKGVFKMDGSKRSAKTNAFFTGFGKSRRIVLYDTLIAKHSVDELVAIVAHEM
                     GHYKKKHILSAMFRKFLQTAFMFWLLSLFIGSEQLFLAFKMPLPPTVYGSLIFFGFLY
                     TPIGILISIIENAISRKHEYAADRYAAETTGKADAMISGLKKLSADNLSNLTPHPFMV
                     WLSYSHPPVLERIRALMEA"
     gene            31873..32142
                     /locus_tag="PKK36_00415"
     CDS             31873..32142
                     /locus_tag="PKK36_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNR93052.1"
                     /translation="MHKRKISAGVVHDVPADLGAALLADSDALSLWENITPLARNEWI
                     CWVEDARKNRTRLKRIERVQTELKAGKRRPCCWSGCPHRSKTRWP"
     gene            complement(32171..34270)
                     /locus_tag="PKK36_00420"
     CDS             complement(32171..34270)
                     /locus_tag="PKK36_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR93053.1"
                     /translation="MTRNILIAMLLAAGAVQAEEKAALPVELPDVMVTATRSEKEAAA
                     LPFTTCLVSAEVMQMEKLSRTSPEALREIPSVMVQKTGHGQGSPFIRGFTGFHTLFLI
                     DGIRLNNSTFRDGPNQYWNTVDPFGIESMEIVKGPGSVLYGSDAVGGTVNALTRSVFD
                     DEADRAVSGRAYYRYSTAEDSHTVRAEGRGTAGGKAGLRAGATVRDFGDLRSGGGDQP
                     KTGYDEVDFDIRAEYLINPNLRLSAAHQTVEQDDAWRTHKTIYGESFHGTTVGDEKKR
                     VLDQERHLSYIAFDAADLSGFIRDLNLTVSHQRQEEDQFRIKKDDTSDIQGYDVDTFG
                     VSLQAISPTKAGEWTYGAEYYRDSVDSYLKKFKADGSLKSIGIQGPVGDDAVYETVGA
                     YVQDVVPLSDRLDLIAGARYNYAQADVGRYEDTAADGPGSLKEDWDEAVGSVRLLYRL
                     LPDGGLNLFAGVSQGFRAPNLSDLTRLDTARTDELEVPSPGLDPETYLAYEAGIRGGS
                     DKASFELVYYYTDMKDMIVRTPTGRVIDEYSEVVKKNAGDGFIHGIELSARRTFAEVW
                     TLWGNCAWMEGEVDTYPTSLPEKKREPVSRLMPLTANAGIRFQPSGKYWLEAVVTAAD
                     RQDKLSTSDVADTQRIPPGGTPGYGVFTVRGGWHFTKNLTMSAALENIADKDYRIHGS
                     GLNEPGRNLVVALDCKF"
     gene            34407..34982
                     /locus_tag="PKK36_00425"
     CDS             34407..34982
                     /locus_tag="PKK36_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR93054.1"
                     /translation="MIRKQKKPDPALLADAEVMKRVRAGHTEAFSVIILRYQNSLLNF
                     FRRLGVYTDAEDLVQECFLRIFRNRMNYRETAKFTTFLYRVGRSVWIDWVRRQARRSS
                     FLTEYEDGMPELNEYCERPAGSGMDAQIALESLPEKLRLVVVMSIYQGMKYAEIAEAL
                     DIPVGTVKSRMSIAFDLLRDYMYADAGEKKV"
     gene            34985..36019
                     /locus_tag="PKK36_00430"
     CDS             34985..36019
                     /locus_tag="PKK36_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93055.1"
                     /translation="MKQPFEELILPYVLEELPPEKRQAFEKEMECNSLLRVRVYETRE
                     MLKKLRELPQDTVSRDLSAEILEAIKSEKKQGNPSWLPRLLKAAACLAVLAGAWLVIQ
                     SMTGGREDPAAAQGFKWIAAAQETSGAWDGARWGGSGKHTIELTSLALLTLASGKSAG
                     MNSDYDEQIRRAAAYLAAHQDREGIIGEGGICSGYGHAIATCALLEVYRHNPSGGLKR
                     VLDGAIRHLQTSHAASGGWAYQTSSGKSVHPTGSAWALLALKRAAAQGWPCSGPPARL
                     PSQPASRIDLYPWYLLSTEFAGADRSNAVRMLELMKENPASDGCLSAGGQIYSTVLAT
                     LTLCAGNPQN"
     gene            complement(36168..37871)
                     /locus_tag="PKK36_00435"
     CDS             complement(36168..37871)
                     /locus_tag="PKK36_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNR93056.1"
                     /translation="MKDMIRSISTLVLVLVVAWGVWMWGFCRFYVEPDSMAVITAKVG
                     KDLPPGQILAGKGQKGVREEVLGEGRHFLNPILYEHEIRSVERIPPGQVGLVTSKIGE
                     ELPQGEFIAARNQKGIWRGVLGPGKYRFNPYGYQIDILDAISIPVGYAGVVTSLSGEQ
                     APEGKFAKLNQKGVREDILQPGLYYVNPKEFKVDVLEIGVNQVSLVGKMGGEVITKGK
                     IEMQNVAMEKLQTKVLEKQQQKRYDYLSQVASSVRARRAMAPAAMQEQAYGDARIPQQ
                     QMADTTAMMGLTEFVEFPSRDGFQISLDMTVEVELLPENIAFIYSRYGDLPAVVDKII
                     LPQITSISRNKGSEYGAKDFIVGEGRQKFQDDLTAALAKTLKAKNLIVHNALIRHVEV
                     PMQILEPIQLASIAVQQDLTNKEKQNTAKKQAELNTELTLIEQRREQVARETEKIKAE
                     IKADQQKQVATIRAEASKSVAEIDKQTALVRADIVRKIGTAEAEAIKMVDGEKAKGLQ
                     LKAKACGDPVAFTMLNMAASLNPDVKINILHSGEGTLWTDLEKATLGDLGGAKIIKPE
                     K"
     gene            complement(37868..39409)
                     /locus_tag="PKK36_00440"
     CDS             complement(37868..39409)
                     /locus_tag="PKK36_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNR93057.1"
                     /translation="MNDIEHSEPRRPGPMPKKPRIPDLSAISWIPVIAVFLLIALPLF
                     IWFGCRIEPRSGEIAVLIRKTGKDLPSGQILAMNPKEKGIQLEVLPEGRYFRNPYTWG
                     WKIHSILDIPAGKLGVKTRLYGEALPVGKIIAPDGFKGVVREVLRPGKYRINPYAYTI
                     ELADAITIRPGHVGVVTALIGDDVLDAELPEPDRNTFLVKEGLKGVLTAVLDPGTYYL
                     NPYEFSVVEVNLQSQRFEMSGADVITFLTFDGFTVTVEGTIEYVLQRDQAALLTHRVG
                     DMDDIIKKVILPRARGFSRIEGSKHPAINFIVGETRQKFQNDLETHLREKCMDWGVSI
                     NSVLIRKIIVPDEIASIIREREIAVQNALKFDQQIEQAKSQAELVKQEMLAVQNKEKV
                     EADTARIAALINARQDQQVRIIAAQQELDVAKLDNEAAAFRAEAVLARAAGDKDAIEA
                     RNLAEASVLASQVSAFGNGMNLARYRFYEKIAPQIGSVLSSDQKQGMGAIFGPLLPEG
                     KGGAQ"
     gene            complement(39513..41543)
                     /locus_tag="PKK36_00445"
     CDS             complement(39513..41543)
                     /locus_tag="PKK36_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNR93058.1"
                     /translation="MKPTVAIMLGMLAATAGRAEVVTLKTGKTLTGEVSRVGKSIVEV
                     RSGKSTSYHSLSDLKPDDAKRIAPDFNPAGGAKPVQRRTPSRAARQETDRPAAPEKPD
                     APAAGKSATLHQVCAAGNVKLAASLIDKGADIAAVDAKGNQPLHAAAAAGQADVVHLL
                     LEKGANVHARGDYERMPLHLAARAGSVEVAEILLSAGADINARSYENEAPLHLAAEGG
                     HAEMIRFLLERDAYPGILSRGNGTALHYAAATGSLSAVEALIGAGLDVNAQDISGRTP
                     LHYAAEQNAADVAALLLDKGAKHNLYGRGYGIHSDAWIQRTPMHVAARFSHPAIVRLL
                     LENGADPHAPDSDGSTPLHEAAAAVTAVYAGRQDEKTEVVRMLAAVCDVNAANDAGET
                     PLICLAQKRAPHPEKSAEILIQRGADVNVEDRTGRTALHYAVAEGYAALVSRLTASGA
                     NQAIRDFDGRLPSDLNPAKKPGKPARAPGLDEPTLLEAARKHDLHLVQHLIARGAAVN
                     EQDEAGNTPLHRAIWGDGINRDDIERLLLEGGADPKKTNAAGRTPLHTAMAVLSVLKV
                     EALIAGGAPVNAQDRNGDTALHLAAAYGKWDLAAVLLKAKADPNIRNREGSTPLHLAS
                     AGRKPEKGIPLLLQHGADPRIMNARGLTPPALARAKGVKDAVAAWEKAGVAP"
     gene            complement(41574..42329)
                     /locus_tag="PKK36_00450"
     CDS             complement(41574..42329)
                     /locus_tag="PKK36_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR93059.1"
                     /translation="MSIKKLLLSALLVMSFAACIPAEEPALAEPDPSLKYLLKSLSDK
                     DWKVARDAQRDILQIGSRAVPFLLEMADGRREGNAEAAISVLGKMGDARVGEPLIRLL
                     QTSPDDKVRKASAEALGRLGETRAVDALMAAASHSEKYLNNRYAAIKALGQIKDPRAV
                     TFLMLIMNGDAYLECRNYAADALDAIAGTQFGHDHRRRMEWIRANHPEWLDLNEEQWF
                     IHPGMIWMAGAVVVFALVGFWLIRSTWNSFRHG"
     gene            42508..43800
                     /locus_tag="PKK36_00455"
     CDS             42508..43800
                     /locus_tag="PKK36_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93060.1"
                     /translation="MTYRKAGIVFLITVLAFSHCPGQDPAPAPGAPVKMSPEAAVLQK
                     LLTAGQIKPGIDYMRKKLAEDPENDRLRYALGLLQFTRAVERLSQSWYRYGLRPPPEF
                     ARELPFLRLPVALNPSPEEADHAAIRGVLQTFLDDLARCRATLGGMKDESASMPVRLG
                     LVRLDLDGDGQAGDGEMLWNIVSRIMQADLPPETVESFVIEFDAADAYWLRGYSHIMS
                     ALLEFWLAHDDAELFSATAHLFFERPNPAHDFLLEERADARAPGRRVVPRHFFDAAAL
                     VHGMISLPLKEPARMHAVLEHLRQVTAMSRESWRCCMAETDNANEWIPNPKQSSVIPG
                     AKVTPEMIRQWMAMLDELDAILKGEKLIAFWRGSQPRGVNIKKVFENPADFDLILWIQ
                     GTAAEPYLEYGPLTDPSFWRQLTESFNGRFVPYAIWFN"
     gene            complement(43756..44760)
                     /locus_tag="PKK36_00460"
     CDS             complement(43756..44760)
                     /locus_tag="PKK36_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNR93061.1"
                     /translation="MKNIGSSLLKLAAAGIVWAGLSAPVQAWAPEGHEIIARIADYYL
                     TPATREKLQEILGSYKLHEYEIASWPDIIRGNKEYEALYPRNGRWHYVDYDAAKRYND
                     DFKLEPPEDGDSIVTQLGRWKDVLSDGRVSTEKRLDALRFVVHFAGDMHQPMHCAYRY
                     GDMGGNMIPVNSFKGKHYSFDADTPMDYAPSLHSIWDEALVNEAMEGLTPFAFAKRLR
                     KEITPEQLRRWRHDDIPAWAIDSYWKARKLAYRWADRNKPVPFKWSRPGMDVTLDNYI
                     NARVPVVREQLQKAGVRLAHLLNEALDPDYRQAAEQAAKEKRSRSVEPDGVGDKSSVE
                     "
     gene            complement(44828..45175)
                     /locus_tag="PKK36_00465"
     CDS             complement(44828..45175)
                     /locus_tag="PKK36_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93062.1"
                     /translation="MASGETVCPEAEESGDSSAPAETPDASHPVRRAERAFGPVLAGV
                     LLDCVDFATMGPFAFIIGIIVGFWIFSIYRLPLQHRIIGALLAGAYCMMPFTRFLPLA
                     TLVGAWVRFRESK"
     gene            complement(45177..49007)
                     /locus_tag="PKK36_00470"
     CDS             complement(45177..49007)
                     /locus_tag="PKK36_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNR93063.1"
                     /translation="MNKTANCFLVSFLLVFAAVLSAHAIPNPAAAYAAKLGYEYSATE
                     DGETVAVADGVVLDAWDFYRGKVGSEYAYGAQFGYDTVNRRVEADGFVQSYAVCVPKA
                     PLKAGGAEEIPLLELMKQNGEPLWDDAMEATPAEIEAAMKTAPLKTFPKMELPSSWDW
                     RNMGGASYIGSVRNQGSCGSCYAFGAAAAAEGTFNYAMGKTGSQCVDFSEAYIAWCLG
                     EYGDYSSHFSGCDGADYDYAELEALTVEGICGENQMPYNNGSDPGSCTYGGTTTVFDA
                     WARVTSGDIDAIKAAIMTYGVVDAAVYVGSAFDAYDDGVYDDSNNSCSSGWYTTSNHA
                     ISLVGWDDNPPEGGGGVWILRNSWGTSWGESGYMRLRYDAAVVHCAVCYMQYTPSSPT
                     PPTFGANPGPLSATTGVAKVFTVTANGYPSPTLALKSTTASSGYNFTAGTGQLSYTAP
                     LADVGSRTFTFTATNTAGAVTQVVSVTVYEAPPAAPASIWASATNTTDFTAAWSSVSG
                     ATGYRLDVGTNGSFSTGGGGAATNLLAEGFASASPSGWTLSGLGTYSSSPYVGTLLSG
                     TYSLKFDGTGDYAITPTFASGATNLQFWAYGNGGAGSTFAISGLVNSVWTLVDTVVIA
                     QNGATYNVALNPQTTQIGFYFTKSYNCALDDVIVQGGAGNPFYIAGYSNRTVASTSQS
                     VTGLTAGMTYYFRARAVSGGGTSPNSSVANVTTYATLSVPVFGANPGPVATTAGLDTE
                     FTVSASGVPAPTLALVETTAAVDSYDFDTGTGDLLYIAPQSDAGSRTFRFSAVNSQGT
                     AYQTVTVNVAAATAPVFTGGAGPYSTTTGVEVAFTVTASGTAPALALQSSTASADNYD
                     FDTGTGYFLYLPTAGEVGTQTFTFTAANAAGTVTQITSVVVSEAPSAPSAPASIWASA
                     TNMQQFTAAWSTASGAASYRLDVSESATFSSGGGGGSNLMTNPGFESGDSTAWTKFET
                     EYSVVTTDPQEGTYHVSCAATGTRDLMQPVAITGDGVTEYEVSFWYKKPGSGNCRIWA
                     SWASGGQVSGDSLQPATYLPTASDWTKVTYTVVPNNGANTLNFEVRTYSGATVYWDNF
                     FVGQAGGGSSPSFVPGYEDRTVAGTSQSVTGLTAGTTYYFRARAVNAVGTSGNSPTGS
                     VTTLESAPPADPYEEWIAGQGENPEDPEFAPEEDADNDTVSNYDEYLADTDPTSPSSV
                     FQGVGSAVLLNQSLTFPASTGRQYSLYYKLDLGDATWLPVVQDQQGTNTTMTLTDTNV
                     RERVFYRLEAELLP"
     gene            complement(49329..52475)
                     /locus_tag="PKK36_00475"
     CDS             complement(49329..52475)
                     /locus_tag="PKK36_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR93064.1"
                     /translation="MKKQIVGVILALLTGPVLHAATVSFGTARVYGEEGAASVSLPVV
                     LSASANATVHVAIAGTALPGGTDFSCSTTLIFSASGSTASNMVFTITDDALAEGPESA
                     RLRLGPVSGVTMGSTTQAVLFVRDNDAFSIVSANLTSGTNEVNSTTTWDDPGGRILEA
                     LCPDVALMQEWVLKPGITYRGFVDEHFGTGFSYYVEYQTGTYAQPNGIISRWPIVDYG
                     EWPDGEVFNRDFAWVKVDLPGSKDLHAVSVHLKASSLETPEEDAATRLAEARALTNYI
                     AQAGWLTNGYVVVGGDLNLTNRTEWALKTLTNIVSDAHQPADQDGDRDTNSGKYRPYD
                     LILPGPSLDERHCSIVCYGETFPNGMVFDTRPSEVTWSGGVPPPALTTDSAAENMQHL
                     AVMKVFELEKDLEAPSTIWASLTNTTAFTAAWNSVPDAIDYRLDVGTSETFMSAGGGG
                     SNLMTNPGFESGDSTGWTKFETEYAVVTTDPQEGAHHVSIIASATRDLTQNVSITGDG
                     VTEYEISYWYKGTGNARIWAAWTTGGQVSGDSLQPTSYNPATSAWTKISYSVVPQSGA
                     NVLFYEIRTYSGASMSFDSFFAGVAGTGGGEPDYVAGYSNRTVSGTSQSVTGLTPNTT
                     YYFRARAVDAVGPGSNSVVASVTTMAEVSGTPPVMDAIPSQNAMVGGEDFEYTVTAAQ
                     TDGDPILAFACTSAVDTNTWDFDTATGDFLFIPTTNETGVNLFSFTASDKDGTSPAVA
                     MTVTVSAAASGASDLFISEYIEGESYNKAVEIFNGTGTAIDLSDYEIRAYFNGSTSYS
                     SVSLNSTLANGAVYVITHGSANAAILAVANLTNSTVMNFNGDDALALYKVSSASFADI
                     FGRIGEDPGTAWTDGSHSTVNQTLVRKSSVTGGITVNPAAGFPSLASEWDSYARDTVS
                     GLGSHSFDGGTGGDTPYDIWADGRGMDPEGPLGGADDDYDSDTMKNYDEYLADTDPTD
                     PDSVFEAAGAAGTLHQVMTFPASTNRRYSLLWKSEIGDAAWIPLLEAVQGTNAIMSLT
                     DTNRRMRAYYRLEAELLP"
     gene            complement(52567..>53815)
                     /locus_tag="PKK36_00480"
     CDS             complement(52567..>53815)
                     /locus_tag="PKK36_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93065.1"
                     /translation="VSYLGDHDFDGGGTPDGTNLPVISYSPAGTNHSVTVSNLLTVTV
                     TATDADGDEIVLVDVSLPSGASFPGDLDTSPVSSDFTWTPTVTGTYVAVFEAEDNDGG
                     VTSTIQIVVNPAPVGGTGTNLLVCDFSSGLPSGWTNIDWLANSPEQKWLFNNPKARMF
                     NASSAGNGFAIMDSDYYGTGKSQAADMVSPVLDMSGATDVVIRFEHYFRNYSTNDWAG
                     IWYNTDGSLTSYVFIATWDGADTANAATYVSNVSSLVSGHSQVRFGWYYQASYDWYWC
                     VDDISITGIVASAGNDDDGDGIPNDWESQYSGTSTGLVASADDDEDDFTNYEEYLADT
                     NPTDPDSVFMGIGAAVALNQVLSFPGSTARMYSLYYRTNIISGPDWTPVVTEVAGTNT
                     LMSITDTNKLDPVYYRLKVELLP"
BASE COUNT        10946 a        16531 c        15441 g        10897 t
ORIGIN      
        1 gtccaccgcc tgcagatatt tatcctcttt cgcaccaggc cgcaggtgaa gccatccctc
       61 gcggagaagc caggcgttca gattgaagcc ctgccggaac gaggtgaagc cgtgatcgga
      121 catgacaatg agcaaatcgc cgtcgcgcat ccgggcggac acttcgccca ccagttcatc
      181 catcttctca tacatggttt cgacaacggc ctccccttct cccccgtctc tcgtgaacat
      241 gtgctgaatg cggtccgaag cgtcaaacac gcacgccacg atacctttct tcacgcgctt
      301 cagcgattcc atcagcatcg ccttccgctc ttcgtgaatg gagtacacct gctccacgaa
      361 ggtatcgtcg cctatcacgc cttcgttgag cgcccaggtg tcctcggcca gacccagtgt
      421 cgcaaacggc cctatcagtc gggccaggta aacggaataa aacgggggat gtgaaaccgg
      481 gagagccggg cgggccggat ctatgttgag cggggacaca taaagcctga aacccttttc
      541 gtccgacgcg aaatagaatc tgcagatgcc gtacgccgaa cggaacaggc cggccctgaa
      601 gcgcacagac acccagtccg aggtccggcc cctgagaagc cggaatttct gtccgcagac
      661 cgcgatgcgg acggcggcat catcctcctc ccgttctatt gcgagcggga cttgaagcgg
      721 cgcgccgggc tgaaattccg gacccggcag gaatgttttc gcgcgaccgc cgataaaatc
      781 cacgcgaacc tgcatgccgc cctcgagcgg cgaatcctgc acagagtcgg tgaagcaggt
      841 aaaagaaccc tgtgtgccgc gcagatcagg cacgcacatg cccgaaaggc agaccccgtt
      901 gaatttctcc ggaggaaaag tgatcggtac gcgaaggatg gtgctgaata ttccatactc
      961 cccggcgacc tgccagaagg gccggctgcg gcggagcagt ttggcgtagg atttgcgacc
     1021 ggaaccgatg cggctggacg acagttccgg aagatacgtg cggagactgc ggttgagaaa
     1081 atcatagata ttgtgcttgc cgggattaac gccggtcatg aacgtggacc aggccacggg
     1141 cgaaatcggc gggcaggtcg aaagaagcgg cttgaagcat ccctgctcct tcagggtcct
     1201 cagttgcgga agacggcccg cctccatcat gcgctctgtg cgttttgcgt ccaaaccgtc
     1261 caaacccaca attaccaccc ggtgaacccc ggttttccgg cgcttgcgcc cccgccgaag
     1321 aagaagcgac agcagcacgc gaaaaggcca gctcaggaca gcggccgcaa ccagcagcag
     1381 ggcggtgagc agaatgaaaa aagagccgac aaacgcaaac ccggcgccgg gaccgatgta
     1441 gcccagcgcc ggaccggaac ctgtgaagaa aaagctcatt cgcgtgaaaa catagcacaa
     1501 accgcgcaaa taggaacagt tcagggtcta tttatttgct gaatgcctgc ctcgcagcgg
     1561 ctataatgcg cccatgaaca aagatctgac atgtttcacc gtgccgcccg ggaaaaagga
     1621 ttcctctttg ctggacttca tcgcagaccg gatgaatctg tcgaagcgca aggcaaaagc
     1681 gcttctggac gcgcgcaacg tgcatgtcaa ccgccgacgg atctggatgg cccagcatcg
     1741 gctgaaagcg ggcgacattg tggaagttca gcaaacaggt ctggccggcg gccacgcgca
     1801 gattctcttt caggatgaag actatctggt ggcagacaag cccgcgggcc ttttgtcaaa
     1861 cggccccgaa agccttgaag accggctcag gcgggaactg cttgttcccg ggctgcgggc
     1921 ggtgcaccgt ctggaccgcg gcaccaccgg atgcctgatc ttcgcaaagc atcctgaggc
     1981 atttgacgcc atgattcccc tgttcaggga gcatggagtc atcaaggttt acaaggcgat
     2041 tgcctccgga ctgattcaga agaaggagct gaccatcagc gagcctcttg agggaaaacc
     2101 ggcggaaacg cgtctgcggg tgctggcggc aaaccagacg gccagttata tttccgtgaa
     2161 gctggtaacc ggccggacgc atcagattcg caaacatctt gcgcacatcg gtcatcctct
     2221 ggccggagaa cggacctacg gcggagggcg gtctgaagat gatgcgctgc gggcaatgcc
     2281 gcgccagatg ctgcacgccg aaaaactgcg cttcacatca cctgtgacag gagcggaagt
     2341 gagctgtgaa gcgccgctcc cgcaggactt taaaagcgcc ctgcgggtcc tcgggctcag
     2401 ttcgacccct gctcgctaac ggcctcaacc gccggcggcg cttccgcagg ctccttcggg
     2461 ctgaaatcca cccatccaat gatgttcagg aaggcgatca tcacggccac ggggctgatg
     2521 aaacggatga aaatgcccca cagggcggcc aacgtgatga catgaacatt tgaattgtga
     2581 gaaggatcgt ccttcagtcc cgccagaaga gaaatcaggt gcacatcggc gaaattgtgt
     2641 gacccgtggc gaatctcatc caccgccttt tgaactcccc atatccagcc gacaaagagc
     2701 gagataaaca gcccgccaag cgggagcatc cagctggacg aaagataatc catcagttcg
     2761 aaaaacgaat ttttggtgtg accgaaagcc ttcagcagcg ccgcatgaac cgaagccagc
     2821 gaattccagt caccgacact cagggcgcag agcgcgccga gcatgaaaat catcaggccg
     2881 aacacgatag aagcctgctt gcgggtccag ttgcgctcat ccacgaaata ggcggtaacc
     2941 acctccagca ggcttatgcc tgacgtaagc gccgcgacaa acagaagcag gaaaaacagg
     3001 gccgcccata acatcccgag gggaatctgg ttgaataccg tcggcagcac ctgaaagaca
     3061 agcccggggc cgacatcggg cgagaatccc atcgcgaaca ccgcggggaa catggcaagg
     3121 cccgccatca gcgcgacaag tgtatcaagc gcgacgatgg acagcgtcga catgaacaga
     3181 ttctgttccc gctggacgta actcccatag gtgataatag cgcccatgcc caggctgaga
     3241 gaaaagaagg catggccgag ggcaatcagg acactggatg ccgtgatctt gctgaaatcg
     3301 ggactgagat agaactgcac tcccttcatg gcgccgggaa gggtcagtcc ccggataatc
     3361 aaaaggacca gcagcagaaa aagcacgggc atcaggattt tggaggcccg ctcaatgccg
     3421 gatttgactc ccttgtatac aataaacacg caggaaaaca tgaagaggaa atggcagccg
     3481 atggaaagat ccggatttcc gatgaacgag ccgaagtggt caccggcggc cgttacattg
     3541 ctgaaatcca gccggtgcaa tacggcttcg atgaaatagc cgatggtcca gccggccaca
     3601 acactgtagt aggaaaggat cacaaatccg gcaatcaccc ccatggcacc caccacagtc
     3661 cagcggaatt tgaaaatgga aaagccgagg atgatcatca tcaggcccca gccccagttt
     3721 ctcatgcaca gcagaatgat tccggccaga atgatcatgg acccgatgaa atgcgccagg
     3781 cgggaggtgg gcggattgag cagtttgaag gcgccgaccg gattgcgctg cgtgtgccga
     3841 ccgaggcaga tttcacacag catcacagga atgccgatca cggcaatgca gaggagataa
     3901 acgataacaa acgcaccgcc gccgttttcg ccggtgatat aggggaactt ccagatattg
     3961 cccagtccaa cagccgaacc ggccgccgcc agaataaagc cgacagaact gttccagtgt
     4021 tcgcgcggtt gacccgtggc actcatgatc acctctcctg cgggaacagc atgcacaaac
     4081 ggcccgggaa aagcctaatc tttgtggcgg aaggtgatgc ggcccttttt gagatcgtag
     4141 ggggaaatct caaccgtgac cttgtctccc gtcgtgatgc ggatgaagtg tttccgcatt
     4201 ttcccggaga tgtgcgcaag gatttcgtga ccattggcca gcttcacccg atacattgta
     4261 gccgggagaa ctttcacaac ttctccttcc acttgtatta ggtcttcttt tccgcctgcc
     4321 atactgctcc tgattttcaa ttaaagcaca gcactctata aatccggaga ctatgctagc
     4381 gggcaggtcc ggtgagtgca agtctgcttt tcatgcacag gagaaaaagc gccggtcact
     4441 gcaaagatcg ctgcagcgct ccaaaccgcg gatcttcgcg aatgatctgc agcagcggat
     4501 cttcgcttcg catcgcctcc ctgaagcgcc gggggttccc gcgcgcccat tgttcgagaa
     4561 gcggccacag ttcgctgatg cggcgcgcgc cggccagcgc ggagatcaat gtgaattcca
     4621 catcgggatc attcggagca aggcggcggg cgcgacgcgc atccgcgatg gctccttcga
     4681 agtcacccgt tttgattcgc acaaacgcgg cgttccgcca ggcgtccacc acctgcggat
     4741 gctgctgctg aaccagctgg agataattga gcgcctccgc ggcgcttccc agcgcataat
     4801 gcgcctccga gagatgtagg ggcacccagt aactttgcgc gcccagatcc tgaagccgca
     4861 gcaggatgtc gcgaaacggc tgccactgat tccgcctttt ggccgcgtag gcgcgctggg
     4921 cggccagccg gacctcgctc agggcggaca ccggcactgg ccccagcgtt tcagaaatca
     4981 gctggtcaaa gcccttttcg ccgagaatct tttcatacgc ctgacggtcc gcctccatcc
     5041 agtttgccag gatatacatt tctgccagag tcagatgatg ccagttgtag ttccgcaggg
     5101 gcttggtccg gccggtggca atgcgcagga caacagcctg aaaatgagcg gacagatttc
     5161 gcggagacaa ctcaaaggcg cggttcagca gggcttcagc cttcggccat gcccgctgac
     5221 ggaccgccac ggccgcccag gcagccacat gcgccacatc gtccggtttc agccgcacat
     5281 agcgctcaaa ataccggtcg gccgcattgt attcgcctgc cagggcgcac agaagcgagg
     5341 ccttcttgag cagatccgtg ttgtcagggg cggcctgaag gacagtttcg atgttgcggc
     5401 gcgcggtctc ccaatcgccc tgtgatatgc tgtgttcggc cgcagcgagc aggtcccggt
     5461 gctgcgccag cgcctggccg gcagccgcct ccgccttccg gtatttttca aaatcctcat
     5521 agctggccgg aatttcggct tcaaaatagg cgtcgcgctc cgcaaagggg ctttccgatt
     5581 caagggtgtc caccgcaaag ccgtccagag aaaacacccc ctgcccccgc gaggtcgcgc
     5641 acacaaaaag cgcagcaaga aggatactca aaatctggag ctgttttttc atatcacttc
     5701 agtccggcaa accatattcg caaatcgtcg agtgaaacgc tgaccaggga atccatcacg
     5761 cgatcggcgg aagccagcgc cggcggcgga tggctgttgg aaaccgccag cacacgcatg
     5821 cccgcccctt gtgccgacgc aatgccggct gtcgtatcct caacagcaag gcatccggcc
     5881 ggagggatcg ccgaagggcg gccggcgttg agccgttcga gtacacggcg gtaacaggcc
     5941 gggttcggct tgctggcctg cacatcttcg gcggtcacga tcgcatcaaa cagggagccc
     6001 agctcgaagc gctcaaggac cggttcgatg tcgccgcgta aagcgccgct gcagagcccc
     6061 agcggaagct ggccggagat ttcccgcagg cggtccagca caccgggata gggttcggga
     6121 ggctctgtgg agatcaggtg ctggaaggca tcggccttcc ggcggatcag cgaggcaagg
     6181 cgggcggaag tcaaaggctt tccggcccgt tgaaagactt cgcggaacgc gtcgcggtcg
     6241 tcgaatccga tgtagtaatc cgcatactcc tcccaggtga acgcaagccc gtcgtcgcga
     6301 agcgcttcac tgaaggcgcg ccagtgcagg ggttcggtat ccacgataac cccgtcaaaa
     6361 tcaaaaataa cggcctgcaa ttccatctcc tgaacttgat tcatgacgat ctgtttaata
     6421 cactccctga cggaatgaat aaagacaata gctcattaaa tgaggcttcc cgtcaaatca
     6481 ccgagcgcat gcaggcggtc cggatgccgc agctggccat tgacacgttt ctgcatcatt
     6541 acgcgaaact tcgggcgggc gcgcgcatgg agattcccga atcggacatc cgtccggtga
     6601 cggacctgcc cagcagcctt gacctcggac agcatgagcc ggcggggcgg agcctgatgg
     6661 gcaaaacagt tatcatcaag ctgaacggcg ggctgggcac cagcatgggg ctggacaagg
     6721 ccaagtcgct gctgccggtc aagaacggca tgtcctttct ggacatcatt gcctgccagg
     6781 tttcggcgct gcgcaggaaa ctcgggttca gcattccgct ggtcctgatg aacagttttt
     6841 caaccgaaga ggattcactg gcggccctgc acaaacacaa ggaattcatc agcggatctc
     6901 cggacattcc cctgagtttt ctacagcatc aggtgccgaa aatcagcaag gccaccggcc
     6961 tgcctgtcac gttcccggca gatccggaaa tggaatggtg cccgcccggc cacggggaca
     7021 tctacactgc cctggtcacc tctggtgtgc tggaaaacct gctttcacgc gggatcgaat
     7081 atgcctttgt gtcgaatgcc gacaacctcg gcgccacact cgatttcagc attctgggtt
     7141 atttcgcgag cggcggatcc ccgttcatga tggaggtgac ggaacgcaca gcagccgacc
     7201 gcaagggagg ccatctggcg cagggccgca gcgggctgct gctgcgggaa attgcgcagt
     7261 gcccggcagc ggaactggac gccttccaga acatcgccgt ccaccggtat ttcaacacca
     7321 acaacctatg gatcaatctg cgcaggctgg atgcggcgct acgggcctca cagggtatcc
     7381 tggatctgcc gctcatcatc aatcagaaaa ccgtggatcc ctgcgacaaa aactccgagg
     7441 ctgtctatca actggaaacg gccatggggt cggccatctc ggcatttgag ggcgccgcgg
     7501 ccgtctgcgt gccccggaca cgctttgccc cggtaaaaac cacaaatgat ctgctcgcgc
     7561 tctggtcgga tatctacgac ctgacacagg aatatcacgt cgtcgtcagc ccccggcgaa
     7621 ctcttggaac catcgatatc tcgctggatc cgtctcatta caaattgatg aaagactttg
     7681 atgagcgttt ccgctgcggc gcgccgtccc tgatagactg cgaatcgctg accatcaccg
     7741 gcgacattta tttcgaagat cacattcgtc tgcaggggcg gcttctgctg aaaaaccggg
     7801 gccggaaagg tgtctgcatt ccggcaggca cgacaatctg cgggcgataa tcaccccaaa
     7861 aggaacacgg cggccttccg gccgccgtgt tcttttttcg acaaggcgaa tgattatttc
     7921 agcgcgttca gaagagcctt caccttatca gtccgctccc acgtgaagtt ttcgaggccg
     7981 ctggtgcgcc cgaaatgacc ataggcggca gtggcttcga atatcggccg cagcagattc
     8041 agctcacgga tgatctgggc gggcttcagc ccgaacaccc tgcgaacagc cttttcgatt
     8101 ttctcttcag gcacccggcc ggtgccgaac gtgtccacca gcacggatac tggctcggca
     8161 aatccaatgg cataggccag ctgcacttca cagcgcgccg ccagttttgc ggccacgaca
     8221 ttcttggcca catagcgcgc catgtaggct gcgctgcggt ccaccttgga gggatccttt
     8281 ccggagaagg ccccgccgcc gtgccgcccc atgcctccat atgtatccac aatgatttta
     8341 cggccggtga cgccgcaatc gccctgcgga ccgccgatga caaaacgtcc ggtgggattg
     8401 acaaggcagc gggtgttccg gctaagcatt ttctttggaa tggttttcat gatcacttca
     8461 tcaatgatcg cccggctgag tttcttatga gtgatgtccg ggtcatgctg cgtggatacc
     8521 accacggtgt ccaccgcgac gggacggccg ttttcatata ccacggtcac ctgcgacttg
     8581 ccgtccggac ggaggaaggg caggcggccg ctcttgcggg tcctggaaag ctcgcggcac
     8641 aggcgatggg catacatgat gggcgccggc atcagctcgc gcgtttcatc acacgcatag
     8701 ccgaacatca tgccctgatc gcccgcgccc tgctccttga agaggccctg cccggccgta
     8761 acgccctggg aaatgtccgg ggactgcttt tcaacggcca ccagaaccgc gcaggtgttt
     8821 ccgtcgaaac caatcgagga atcagtatat ccgatccggc ggatggtgcc gcgggccacg
     8881 tcggcatagt tgatcgaggc ggtcgttgtg atttcgccgg caatgatggc catgccggtc
     8941 ttgaccagcg actcgcaggc cacacggctg tttttgtcct gccgcaaagc tgcatcaaga
     9001 atcgcatccg agatggcgtc gcaaaccttg tcgggatggc cttcggtcac cgattcagaa
     9061 gtgaacaaac atctttttga catactcatc acctttcttt gaaaaaccaa aggggcactt
     9121 tggcaagctc agaccacaaa atcaagcaat tcgggcgcaa tctgttcaac cagtttccgg
     9181 cactgcgcga gaatttcgga agcggaccgg gactggagcg cggcttcgcc gagttccagg
     9241 gcctgggaat atttcaggca gcggataatg cgcttgacca tcgggaccat gaccgggctt
     9301 acgcttagtt catcggcgcc aaggccgatc agcagcggaa caatcagcag attggcggcc
     9361 atttcgccgc acacgccggt ccagatgccg tgctcgcggc cggtgtctat cgtgtgcttg
     9421 atcagccgga tgatggccgg atgggcgggt tcatagagat aggccacccg ctcattcacc
     9481 cggtccaccg ccagcgtgta ctgcaccaga tcattggtgc cgaggctgaa aaaatccaca
     9541 tggggcgcaa ggatgtccgc cgcaagcgcc gcggagggga tttcaatcat gatgccgact
     9601 tcaatgtttt cgttgaaagg cttgccttcg gcccggagtt cctgcttggc cttctccagc
     9661 acctcattgg cctgcagcac ctcatccaca ttgctgatca tcggatacat cagccggatg
     9721 ttctcgtgac ggctggcgcg gaggacggcg cgaagctgcg tcttgaaaat atcctgctgg
     9781 gccaggcaga agcggatcgc gcgccagccc atgaacggat tgacctccag cggggtgttg
     9841 atatgggata caaacttgtc gccccccaga tccagggtgc ggatgatgac cggatgcggg
     9901 gccgtgcggg tggccaccga ggaataggcc tccacctgct cgtcctccgt cggcagatga
     9961 tcccccgaga gatacaggaa ctctgtgcgg aacagtccca cgccggcggc gccgtggcgg
    10021 atcaccgcgt cgctgtcgct cggcagttcg atgttggcgg acagaattac acggtatcca
    10081 tcgagtgtct ccgcgctttc gccctgaagc gccgccagtt cggaacggat atgccgctgc
    10141 tcctgcgcca gtttcccgta gtaggtcagg cgctgggccg tcggattgac aatcagcagg
    10201 ccccgggtgc cgtccaccag cacaacggcg ccggccggga cacgggtgct ggcatcgtgc
    10261 agcccgacca ccgccgggat ttccagtgca cgggccatga tggcagtgtg cgaagtgtga
    10321 ctcccgaggt cagtcgcaaa accaatcgtt ttgtcgcggt tgagcgttgc ggtatcagag
    10381 ggggcaaggt cgctggccac aatgatgcat gattcctgca gccccgcgag atcggaaagc
    10441 ttgtgcccgg aaaggttcct gagaatgcgg cgggccacat cgcgcacatc ggcggcccgc
    10501 tcgcgcagat agtcgtcctg cacgcgcgaa agcacgtcgg cgaattcctc ggtcacgcgg
    10561 taaaccacag cctccacatt tttcttctgc tcggagagcc cggcgacaac cttctccacg
    10621 aaggagtggt catccaccac catcaggtga gcgtcgaaga tgctggcctt gcgctcgccc
    10681 agcgccttgt tcacctgttt ctggatttca tggatctgct ggcgggtggc gatcagggcc
    10741 tgttcgaagc gggcgatttc cccggggatg tcctgctcgc ggatatcgtg ctcgaccagt
    10801 cgcagcactt cgggtgcaat caggtaggct tcgcccacgg cgacgccggg ggaaacccca
    10861 attcccttca ggacaagctc ttcatgtgca gccggatcag gcatctgccg ctaatcctca
    10921 aaaaatttgt tcttcaccag ttcttccagc gcgtccagcg cctgcggagc atccgccccc
    10981 gtggcgcgga gtatgagcac cgagcccatg tatccctcca gcgtcaggag gcccataatg
    11041 cttttggcgc agacacgggt tccgtctttt tccaccgtga tgtcggcgga aaactcatta
    11101 gccttcttta caaagagcga cgccgggcgg gcatgaattc ccaggcgatt gacaatttcc
    11161 accttccgga cgtgctcaga cactgtatct gtccgggagg ccgtcacatc cggctctttt
    11221 ctgttaaaac attcaccagt ttctgatcca gttccttggc ggcatcatgt cccatccgct
    11281 tgagtttctg attcagcgcc gcgacctcaa tcacctgcgc catgtcgcgg ccgggcgcca
    11341 cggggagacg aatcagcggg actgaaacat ccatgaaagt catggtttcg gaattcaggc
    11401 ccgtgcggtc catatcggcc gcgggatccc acggctgcat ctggatcacc aggtccaggc
    11461 gcatgttgct gcgcatggag gccacgccga aaagactggg cacgtgaata atcccgagcc
    11521 ccctgatttc catgtgatag cgcgtcacct gcaccgcgga gccgataacg aaaccctcgc
    11581 tgtcgcggcg cagcacggtg acgtcatcgg ccaccagact gtggccacgc tcgataagcg
    11641 ccagcgcagt ttcactcttg ccgaggcccg gcgggccctg cagcagcacg ccaatgccca
    11701 tgatgtccac catggtcccc tgcacgcgga tttgcggagc ggccagattc tccatgatga
    11761 tggtggccgc attgatgaag cggctggtga tcatcgggga gcgcatcacc ggaatctggt
    11821 attcctgcgc ggcggacatc agttcgggaa tggcatgccg gttgcgggtg aggaccatgc
    11881 aggggatatc gcgccggcag acctcgcgga tgcgttccag cctgagtgcg gccgtcaggc
    11941 tgcgcagata gtcaaactcc gccaggccga tcacctgaat ccggcgatat gaaaaatgcc
    12001 ggaaaaagcc ggtcagcgcg aggccgggcc ggttgatggc ggattccgga atcgggcggg
    12061 gaaaatactc cccgccgaat tccagctcca tctgcaggct ttccttccca gcctcataaa
    12121 actgtccgac actgacaggc acaggatgcc tttcccctga gatgatgctt caggcttctt
    12181 ttttactgac cttgacttcg gcttcggcca ggggctcggc gcctttgtga tcctgcacct
    12241 tgtcgcgaag cttgcggagc tggcgcatgg ccttttcaat ggcgctgtcg atggatgcat
    12301 acatgtcatc gctttcatca tgcgcatcaa tgcggatatg gttggcaccc tgcaccacga
    12361 tttctgcgcg gtgcctgtat ttttccacac tcaaaatcac gtgcacactc tccacgcgcg
    12421 ggaagagggc catggcgtcg ttcacccggt cttcagcata ttcgcgaatg gcactggtta
    12481 tgtcgacatg tcgtcctgtc actgtgattt gcacaacacg actcctttct ggttgtttac
    12541 atgctgaacc gcggtcccag atacagatcg cggctcacct tgtcattgat caaaaattcc
    12601 ctggttcctt ctctcagcac ttttccgtcg cacagcagat aggcccggtc caccaccgcg
    12661 agggtctcgc ggacattgtg gtcggtgatc aggatgccca ggcccttttc cttcagttcc
    12721 atgatgatct gctggacatc atacaccgcc agcggatcca cgccgctgaa cggctcgtca
    12781 agcaggatca gcgtcgggct ggtcaccagc gcgcgcgtga tctccagccg gcggcgctcg
    12841 cccccgctga gggtgtaggc gcgctgtttg gccagatagg cgattttcag ctcgttcagg
    12901 agaaactcca ggcggtccct gcgctcctgc ttgctcaggg ggagcgtctc gaggatggcc
    12961 atgacatttt cctccaccgt cagtcggcgg aaaatggacg gctcctgcga gagatagccc
    13021 atgcccatgc gggcgcggcg gtacatcggg gtgcgggtca cctcgcggcc cgcaaaatgc
    13081 acacggcccg cggtggggcg gatgagcccc ataatcatgt agaagctcgt ggtcttcccc
    13141 gcgccgttgg gccccagcag gcccacgatc tcgccggggc ggacattgat gttcactccg
    13201 ctgaccaccc tgcggccgcc gtagactttc tccagctctt cggtgctgac cagataagtg
    13261 ctgtcggtca ccatcctact ctcccgaaac ggtctttgca cgcgatgtct taccgctctg
    13321 tggatatatt accagccgcg cgcgcggttc gcaaaccatt ttgttcaggt cccgccagaa
    13381 ggagatggtc gtgccctcca gcacgtcgtt cccgcgcgtg acgcgcggtt tctcatccag
    13441 ctgaatcttg ccgctctcga catcgtagac cgcccggccc gccgtggccg tccgatcctc
    13501 ctgctcaatg atcacattgc cgcgcgcgat gatggacttg acttcgttgt cctccgcgaa
    13561 atgcacggtc atccggtcgg ctttcagctt catttcgggg tccaccacca ccacgttctc
    13621 ctcaaagagc gcataatgct cgttatagtc gaaggtgagg cgctcggagg tgatctccgt
    13681 tacctccttt tttccgccct gcgcgaaaac cgccggcgcg caggcggccg caattcccgc
    13741 cgccaaaatc aaaagggtcc gtcgtctgct cattttttgt ctcccggatt tactctgctt
    13801 cgaaaacctt tcagcaccac tttggtgtcg tgcatgattt gtatgcgttc cttctcattc
    13861 gagaaatcga accccctgcc ggtgacgacc atgttgtcgc ggatgatgcg cacggggccg
    13921 tcggatacgg cctgtttggt ctgctggttc agctcgcagc ggggtgcgat gaccttcatt
    13981 tcaacctgct cattgttgta gatgtccacg cgcaggttgg aaatgcggat cagcccgccc
    14041 gggaccattt cggccatgtc cccgaagatg tgccgcacga ttttcccatc ggcgtcatat
    14101 tccggcagct caaagccggc gagcgactgc acgccgggat cctgcgccgc ggccgcggcg
    14161 cagaacacaa acatcagcag gaggttccaa ccctttttca ttctgcatcc tcggtatatc
    14221 ttttcattgc ttcctcccag aggccccggg ccctcatcac cagttcaatc acctcgcgcg
    14281 cggcgccttc gccgcccttc cgcgccgtga cggcgtccgc ggcggccttc acctcgtcca
    14341 cggcgttggc cacggccacc gacaggccgg ccagccgcat cggcggcagg tcaatcacat
    14401 catctccgac atagcaggtc tgccccgcgt tcactttcat tttccggagc aggcgcggca
    14461 gggcctcgcc cttgttcatc gcgccctgct cataaaactc cacccccagc tccttcatcc
    14521 ggcggtccac caccgccgac gagcgccccg tcagcacccc gagtttcagg ccggcccgag
    14581 cggccatttt gaagccgtgc ccgtcctgca cgttgaagat tttccactcc ccttccggcc
    14641 cgtaaacaat cgcccccgac gtcagcaccc cgtccacatc aaaaatgatc gcctcgatcg
    14701 cggccgcttt cttttcaact gacgacggca tgaatccccc gcagctgctt ccagagtttt
    14761 gcgatgccgg caaaccggat ggcgttgtcc cggtcggaca gcgctttttc cggatgcaca
    14821 tgggtttcga ggaatacgcc gtcgcagcct gtcgcgacag cagcgcgcgc cagcgcgggc
    14881 gcaaactgcc cgtcgccgcc ggtgcggccg ccggctccgc cgggcttctg aacgctgtgc
    14941 gtggcgtcaa agacgaccgg atagccggtt tcgcgcatga tcagcaggct gcgcatgtcc
    15001 gccaccagat tgttgtagcc gaagctggcg ccacgctccg tcaggatgat tttccggttt
    15061 ccggtgcttt ccaccttgcg gatcacgttc acaatgtccc acggggcaag aaactggccc
    15121 ttcttgatgt tcacgggcct gccgctctcg cccagcgcca gcaccagatc ggtctggcgg
    15181 cagagaaagg cgggcagctg cagcacatcc gccactttgg cggcatgcgg aacttccgtc
    15241 tcgctgtgga catccaccag gatcgggagg ccgaagcggt ccttgacctc gcgcaggatt
    15301 tccagaccct tcaccatgcc cggcccgcgg aaggcgtcgc cggaggtccg gttggccttg
    15361 tcgtaggagg ccttgaaaat cagcggaatt ttctcttttt ccgcaagctt cgccaggcgc
    15421 cgggcaagat caagacaaat ggcgcggctt tcgatgacgc acaggcccgc catcagcacc
    15481 agcggaccgc cgccgcccac cccgacctgt ccaaccctga ctgtctttgt ttttgtcatg
    15541 cgctgttccc aaattgcgtg gctaccataa aggctccggc ggaagaaata aagctttttg
    15601 cgcggcggcg ggaaattgtc cctttcgcca gttttttgtg aaaatctggg taaaaatggc
    15661 aaaaaatcgc gaaaaatcaa gaaaaatcgc cattttttgc gatttttggc cattttttgg
    15721 gcttttttga cactttttta gcgtttttgg cctgtttttt atgtttttca ggaccagcgc
    15781 tcaacatagg cctcgatgtc aaatttgcgc ttgaggccgt tggcgttcat gcagcggatc
    15841 ttaccgaaga cgggccgctc ggtccaggcg cggtcatgcc gcccgaagca ccacgacacc
    15901 cccgcaaagc cgttcggatc gcgtccgtcg agttcatatt tattgttcag gtgcagcgcg
    15961 gtttcaaatg cgtcttcggg cgtccggctc cactcaagga tttttttgcc ccagtacatg
    16021 cgcatgtaat tgtgcatgaa gccggtcttt gccagttccc tctgcgcggc attccagtac
    16081 gggtcatgtg tggcggccgc ctcccactgc tcgcgcgtgt aaacatgttc ccgcctgtcc
    16141 ttcgcatgct cccccatcgt cttcagcgcc cacgcgggga gcgaatcata tttgtcatat
    16201 tccggattgt accagcagaa gttcatgctg agttcgcggc ggacaatgag ctgctccaaa
    16261 caggcgtcct ttgccgcgcg gggcgcatcg gaccctgaaa tctgcagggc catgtccagc
    16321 ggcgatattt gaccaaagtg cagatacgga ctcatgtgcg aacaggcatc cagcacggga
    16381 tcgctgctgt tttcatgata ttcatcaaga tgttgtgcaa tgaatttcga aagctgtttc
    16441 cgggcggcat gcgagccgcc cgcgaatcgc gatgacggct tcacggaagc atccgttttc
    16501 aatccggcgc aaacggcatc aatgtcttca agcgaaagcg acgcgggcgg tttcccggaa
    16561 aatggatatt tcacgggcac ctttcggagc ggctccagat aaagcggaag tattctgtga
    16621 attttcggcc gaatggttcg cgcgccaaac tcttcgtgat ccgaaacctc ctgcaccgga
    16681 acgatcacat ccgtctcaac ggcctccatg cggcaggccg cgcggccggc cgcatccctg
    16741 cgccactgct tctggatgcg agtatagccg acatccgcaa ccagcagaga agccttgcgg
    16801 gccagttcct gaacaacatc ggccggcgcg ccacgcctga cacagaacgg aatgcccctt
    16861 ttcgcaagct gaccccggac atcgcgcaga ccttccagca tgaaccggta gtggcgcaga
    16921 ttggcctccg ggtacgaggt cagcgcaaag cagaccacca gcggctgctg cagggcattc
    16981 gcctgacacg ccgcatgctc cagcgcatga ttgaactctg cgcgctgcgc ctgctgcatc
    17041 cagtacagga catattcacc cccgccggcg gggatgctgt tcagagcatg gatgcgctga
    17101 acctgaatca tggtcagact tccaggttgc cggaaacaaa cggcacctga tgcgaacgcg
    17161 cgatcaggtt ggcggcaatc cggccggact cgtagatcgt cggcaggccg cttcccggat
    17221 gggtgccgcc gccgaccaga taacagttcc cgacctcctc gaacttgttg cgcggacgga
    17281 aatacagcat ctgcgtcagg ttgtgggcga ggttgaaggt cgcgccctcg tacacattga
    17341 ggtcatcgcg ccattcgcgc gggccaatga ttttttcctc cctgatgtgt gaacggatgt
    17401 ccttcatggg cgtgcgctcc tcgatgttct tcagcaccac ttcgcgaaat gaatcgcgct
    17461 tggcggccca gtccacgggc gcgcgcaggt tggcaaccgg aaccagcaca tagagggcgc
    17521 tgtgccccgg cggagcgagc gtcggatccg tcagcgacgc attgcggaca tagaaggaaa
    17581 tgtccgttcc ggggtcgcgc agatggaaca cgtcctccac attgcggcgg tagtcctttg
    17641 cgaagaacac ggtgtgatga ggcaggtcgt atgacttgtc cacgcccaga tacatcatga
    17701 aaatggagca ggacagccgc atcttctgca gcttctgcgg cgtccacttg cgcaggaccc
    17761 cgggttcaaa cagattctgc gccgcatagc cgaagtccgc gttgatcacg acatcgtcgg
    17821 cctcgatgac ctcgcccccg gcaaggcgga cgcctttggc ccggcggtct tttacaatta
    17881 ttttttcgac gggtgcgctg agacggattt ccgcaccgtt cgcgcgggcc tggtcggcca
    17941 tcgtttcgga aatgcggctg agaccgccga tcggatgata gatgccgaag ccgtgctcga
    18001 tgtagggaat gatggaaaac agaccggggc attcccacgg cgacatgccc agatacttgg
    18061 cctgaaaagt gaacgtgagg gcaaggtccc ggttcccgaa gattccgtac atgacatcga
    18121 aaaggctctt cccggcggcg atgtacggga gggccttgat catgtccggc gcaaacatcg
    18181 tgcggaagcg atgataggac ttctgcaggc acgggaacaa atagcggaac cgcgtctgct
    18241 cctgcttgaa gaacttgtcg tagcggttct catgacccgg gaaaacgcgc gcgatctcgc
    18301 ttttcatctt ctcgtggtcg gtcgaggttt cgatgtactt gtcctgatac tgcaggcggt
    18361 acatcggttc gaggcggcgc atgtccatgg ccttgtccgc gtcgccgccc gcctcacgga
    18421 acacctcatc cagaatatcc ttgagcatca gaaaggtggg cccgatatcg aaggtgtacg
    18481 gtccaagcct gatggcgccg ttgcggccgc ccacgtgcgg ctgcgcctcg caaacggtga
    18541 cgcggaatcc gcgatgcgcc agaatcatgg ctgctgtcag ccctcctggc ccggccccga
    18601 tcacaacaac atgtttcttc tcagcctgct ctgccatcag tgcctcccca tattttgaac
    18661 ctgtacgata tcagcccgtc agccagcggt aaatcttttt gaaggttttt agcgtcatcc
    18721 catagggcgg gtacgcctgc ggcatgtccg gccgggctcc gcgcctcact gtcccgcgcc
    18781 agtgcgtgaa cgcgtcgaac ccggcgcggc cgtgatacgc ccctatcccg ctttcgccga
    18841 tgcctccgaa cgggagtccc tcaacaagaa gatgactgat tgtatcgttg acacacgccc
    18901 cgcctgaggg aatgcgcgcc agcaggtctt cggccagctc atttgatgac gagaagacat
    18961 agagcgcaag cggcgagggc attttctgaa tttccgcaac cgcggtatcc agtgaactgt
    19021 actcaatgac cggcagcacc ggcccgaaga tttcctcgcg catgactgga gcgtcgagcc
    19081 ggggattcag cagaatggcg ggcgcaaaat atttcgatgc cgaattatgc tgtccgccat
    19141 gcacaatcgt ctgaccctca agataccgcg ccagccgctg aacatgcttc gcgctcacaa
    19201 tgcggccgta atcggggctg gtttccgggt ccggaccata aaactccgta acggcctttg
    19261 cgatctcctc aacaaagccc cctgccgccg accggtccac ccacacatga tctggagcca
    19321 cgcaggtctg gcccgcgttc atgaattttc cccgcgcaat cctgcgggcc gccaccggga
    19381 tatctgcgtc ggcgcagacg atggcgggac tcttgccgcc gagttccagc gtcaccggcg
    19441 tcagatgcct tgcagcggcc gccatcaccc tgcggccggt ctccgtgccc cccgtgaaaa
    19501 atatataatc aaatctgttt tgcagaagag tttccgcggc ggtgtaatcg ccctcaacaa
    19561 cacgcacatg gtcgtcggga aacgcggccg acaggacatg cctgatcacg gcagaagtgt
    19621 gcggggcgta ttccgacggc ttgatcaccg cacagtttcc agcggccacg gccgcgacaa
    19681 gcggagaaag caccagctgg aatggatagt tccacggtcc catgatgaga accaccccgc
    19741 gcggttcggg aatcagtcgc gcccgacccg gccacagcat cggaggcacg gactcgcggc
    19801 ggggccgcgc ccagcggcgc aggtgtttca tggtataacg gatgtcgcgc aggacaaaac
    19861 ctggttccga cgcataggct tctatggccg gttttcccat atctttctta agcgcatcaa
    19921 gaatttcggc ctcggactgt tcaacagcgc gggcgagcct ggacaggctc tgcaggcgaa
    19981 acgcaaggtc gcgggtgcgg ccggccgcga aatattcgcg cattttcaga tcgcaaaagc
    20041 tttctgtcat gtcgcactta tagcgcttat ccggcaggtt gtcagatttt ttaatatcca
    20101 tgtgcgcaaa aagcggttat tttcccgcaa gaggaaatgc acgtcttgcg acaaagagga
    20161 aacatgacat ttgcggcggc aatgctggcc gccctgcttt gccccctgtt cgcccccgcg
    20221 cgcacgccgg cacatgtgct tcagggaatg gcccttcttg aagagggcat gtcccggtgg
    20281 gatcccgtcc ggctggaaca ggcgctggag catttccgcc gctccaccgc atcgaatcct
    20341 gaacagtttg aaccctggta tctgaaggga ctcgcggagt tccatctgat gcttgtccat
    20401 gaaagcaggg cggctgaagc cgacaaggcc ctgacggcga aactgcgaaa ggacgcggag
    20461 cattcgatgg acaggacccg ggcactcaag caggatttcg gcgaagccag cgcactgaag
    20521 gcggtcctga tgggcatggc catcgcggag catccggcca ccgcaatctg gcgcgggccc
    20581 cggctgcagc atctccagaa ggaggcgctt gcgctcgacc ccgaaaaccc ccgcatctgg
    20641 tacctgatgg gaatgagccg ccattttgcg ccccgtccgt tcggcagccg gaagaaagcc
    20701 cgcgaatatc tgcttcgagc agcagaactt ttcgaacacg accagcccgg ccgacgcgtt
    20761 ttcagatatc ccgcatgggg acacgcgcac tgcctgatgt ttcttggaga aatcagccgc
    20821 gaagagaaca acaaccggga ggcggccgag ttctatcgcg aagcgctcgc cgtcaaccct
    20881 catctggacg ggatcaggaa actgctgaag gagatacaaa aatgaacagg cataaaaaag
    20941 tcgccgtcat cggttccggg ctggggggaa tttccgctgc catctccctc gcgcaggagg
    21001 gttatccggt cagcatttac gagaaaaacg accgcatcgg aggcaagctc aacgtattaa
    21061 gcaaacaggg ctacacgttt gacatggggc cgtccattct gaccctgccc catatctttg
    21121 agcgcctgtt cacccggtcg ggacggaaaa tgtccgatta ctttcccatt cgccccctga
    21181 cgccgcattg gagaaacttc tttgacgacg ggaccgtcgt gaatctcttc atggaaaagg
    21241 acaggatggc ggctgaagcc gagcagcttg aacccggtgc ggccaaacag gtggaggcgt
    21301 tcctgaacta tgccgctgaa caatatgatc ttgtggaaaa gggctacttc gagcacggcc
    21361 tggacaccat tcgcgatttc atgaagttct acaccggctc gacgttcttc aagttcgacc
    21421 tgtggcacac catgcatcag ggggtcgcca agcggatttc aaaccgttat ctgcaggaca
    21481 tttttgattt cttcatcaag tatgtcggct catccgccta tgatgcgccg gggtttatga
    21541 actgcctgcc ggtcattcag ttccgctatg acctgtggta tgtggacggc ggactctacg
    21601 gcatcgcccg ggggcttggc cgcctgatgg acgagctggg catcagggtg cacctcaatt
    21661 ccgaggtaac cgccatccgg aaaagcggga acagggtcac aggcctgaca ctggccgacg
    21721 ggacggctgt ggatgccggc atcatcgtct ccaatatgga ggtgattccg gcctgccaga
    21781 gactgcttca cgaggacgcg gcattcatga agaaacttga gcgctttgag ccggcgtgct
    21841 cgggacttgt gcttgatctc ggcctcgact gcaaatacga ccagctggcg catcataatt
    21901 tcttcttctc cagcaaccag aagaagcact tcaaaagcgt tttccgcgac aaggtgctgc
    21961 cccataatcc gacgatctat ctcgtggcgg catcccgcac ggacccgacg gtggctccgc
    22021 cgggctgcga atgcctgaag atcctccccc acatcccgta catcagcgac agcaatccgc
    22081 tctcgcagga ggattatctc cagttcaagg agcatatcat cgaccgcctg gaaagcatgg
    22141 ggctgaagga tcttcgaaaa cacacagtct ttgagcatgt ctggaccccg ctggatattc
    22201 aaaggcagta ctattccaac aagggctcca tctacggcgt ggtatccagc atgacgaaga
    22261 acctcgcgtt caaggccccg aagcagagca cgcgatatga caatctcttc ttcaccggcg
    22321 ggtcggtcaa tcccggcggc ggcatgccga tggtggtgct ctgcggccag aacgtctgca
    22381 agaaaatcat cgaatgggac cggaactaac attgtggctt cttgatctgg gcatctggtt
    22441 tgccggcatt ctgatgctct ggcgcatccc tctctgccgc aaacgcccgt catccgcgcc
    22501 ggccccgtcc gtcatcatcc ccgcgcggga cgaagagcgc aacctcccgc gcctgctgga
    22561 atcgctgaag cgccagattc ccggcccgct tgaaattatc gtcgtggacg atcactccac
    22621 tgaccggacc gctgaaattg cccggcaggc cggggcgcgc gtgacaggtt ccgcaccgct
    22681 cccggcaggc tggaccggga aaacctgggc ctgtcatcag ggggcgcagg cggcacagag
    22741 caaaatactg atcttcatgg atgcggacac ctggctggaa gacggcgggc ttgccggaat
    22801 cacctccaca tgggaagaga aaaaaggcgc gctgtccgtg attcctttcc attttacccg
    22861 caaaccctat gaatggtttt cggtcttctt caacgtcatc atggcggcct ccatgaatgc
    22921 gttttccatc tggtcgcgca ctgaccgcag cgagggactt tacggccagt tcctgatggt
    22981 ttcaaaagag gattacttca aaatcggcgg gcacgaaagt gtgcggcagc acattcttga
    23041 aaacctgcat ctggcacccc tcttccgggc ggaaggaatt cccgtccgct gcgttggggg
    23101 ggagagaagc ctcgggatcc ggatgtatcc cgacggtttt gcaagcctcg cgggtggatg
    23161 gcgcaaagcg ttcggttccg gagccgcctg cactccgcgg ccgctccttc tcctgatggt
    23221 tgcctggctt gcggcggcag cgggaacagc cctgatgctg ctgctcgcgc ccctgcgcac
    23281 ggcgggaaca ggcattgcat ggcttctgct ttatctgctg tttgtcatcc agatgcggtg
    23341 gcacttccgg cggattggga acttcccgtt ggccgccgcc ctgttctatc ccgttttcct
    23401 ttttttcttt ttctggatca gcgccctccc gcagcaggca aactggaaag gccgcactgt
    23461 cggggccgcg gggaatgaat catgatcatc catctgccgc cattctggat tgccggttta
    23521 aatgtaatcg cctgccttgt tctgcaaacg ggcatcgcct ggggcgtcac acagatcaga
    23581 gcgagcagat ttcgtccaga tgcatggctt taccgcaagc gggaatggga acggaatgga
    23641 cacatttacg aggatctttt tctcatccgc aaatggaaaa ctcttcttcc ggacggagcc
    23701 gcctggttca agaaaggatt cgcgaaaaag aggctgaatg aacgcagtgc cgaatactat
    23761 cgccggttcg ggatggaaac ctgccgcgga gaactggccc actggctgca gatcgcctgc
    23821 gccccgctgt ttttcctctg gaatccggcc tgggcgggat gggccgttgc gagctttctt
    23881 cttctggcga attttccatg catactggca cagcgttaca accgcattcg actggcttcc
    23941 agccgcatgt gccgccggtg aattttcgtg ttttttccgg ctgctcgagt atctttaacg
    24001 gggtgatcat gaagcccgga agattaaagt ggattttggg agccatgctg ttcgcggctg
    24061 tggccgtgta ctgcgcatat ctttttatgc cgcgacccgc gccgaagaat gtgccccgcc
    24121 tcgccgcagg aatggaaatt ttccggaacg ggcagagcag cagagatgcg gccgtcatgg
    24181 aaaaggccct ccgcctcttt gaagatacct gcatgaagga gcccggttcc tatgaggcct
    24241 ggcatatgaa aagcctgacg gaactgcatc tgcttctcca cagcagggat gtccgcgaca
    24301 cggagggtga ggccgtattc agcagggcgg cggaacactc cgcgatgaaa gtcacggcgc
    24361 tgcaaagcac atttcccgaa gcctatgcca tgctggcggc ggtaaccgcg ctgcggcccc
    24421 cgcagcgcgg gcaaaccgcg gcggtcaggc gctatgaaaa acttgcactg atgctgggcg
    24481 ggaacaatcc gcgggtgcat tatctcctcg ggctcagata ttatctgagc cgcggccgcc
    24541 tcagaaatcc ggacagggcg gaagaactgt ttctgaaggc cgccgggctt tttgagaggg
    24601 aaccgcccgg cgcaagagcc ttcaacttcc cctcatgggg ccatgcggcc agcctggagc
    24661 atcttgggga cattcatgag gaacggggcg atgttcaggc ggcggccgga tactaccgcc
    24721 gcgcccttgc ggtaaacccc tttcttgagc ctgtgcgccg ctcacttcag gacctgaaag
    24781 atgcccgccc cggcactgcg ccgcggggga agagccctta ggcgcgggat ccgcggccgg
    24841 aataatgctc gtagccccgt ttacccgtca gcagcatgcc gccccgttcc gtgcggcata
    24901 ctatggaccc cgttctgccg gtgatcctcc cgatcctggc gcacgataat tcaaattccg
    24961 cgcgccaggc ctgctcaaag ctgtccgcat tcccgggcgc aacagtgaaa agcagctcaa
    25021 aatcctcccc atccgtcagc gcatgatcaa gcggagacac gccgtcgttc atctcgcgcg
    25081 cggcgcggga aaccggaagc gcgtccggct ccagttccgc tccggcgcgg ctcatctcaa
    25141 ccaaatgccg cagatccgtc gcgaggccgt cgctcagatc aatcatggcg gtggcccagc
    25201 ggttttcccg gagccagatg ccttcagcca cgcgcggctc aaagaagaga tgcttcttcc
    25261 tgatactgcc gcccagttcc ccggtgacat acagaatatc gccctcggac gccccgcccc
    25321 ggcagacggc ggtgccccgg ggaagcttcc cgaccccgaa cacatgcagc gccagcgtgg
    25381 cgccgctggc cacatcgccg ccgatgatgg ccaccccgaa cttgagcgcc agcgccgaag
    25441 cgccggcgta gatctgctgg agcttgtcaa aaggcatttc cggcggcgcc acgatgttga
    25501 tcagcagcca gagcggctcg gcccccatgg cggcgatatc actcagcacc cggcccacgg
    25561 ccttgttccc gatccccttc ggcggcgcgt ctatcgtgaa atgcgtgccc tcgatgacgg
    25621 gatcggatgt gagcacgaga tccgcattga acccctcgtc atgcatgacg gccgcatcat
    25681 ccccgatgcc caccacctct gcaacggccg ggaaaagctt tcccgccagt cggcggatcg
    25741 cctcgcactc accaatgtcg cgcagtgttt tcacttcatc cactcccgca gtgtctcgcc
    25801 aaagggcgga taggccgcac ccttctccgt cacgatggcc gtgatgtatt cggccggtgt
    25861 cacatcaaat gccggagcat acaccttgac cccctccggc gccgtccggc ggccgaaacc
    25921 gcaggtcacc tcttctgcgg accgctcttc aatgggaatg cagtctccgg aaggcaggga
    25981 catgtcaatc gtggtggtcg gagcgagaaa atacacgggg atgccgtggg cgcgcgccag
    26041 tcgcgccacg ccgtaggttc cgatcttgtt ggccgcgtcg ccgttggccg ctatgcggtc
    26101 cgagccgacc atcaccatgt cgatcttccc ctccttcatt accacggccg cggtgttgtc
    26161 acagatgagc gtgacatcaa tcccggcctg catcagctcc catgcggtca gccgcgcacc
    26221 ctgcagcagc gggcgggttt catccgcgaa cacacgcacc ctcatgccgt gcgcctgcgc
    26281 ggcatagatg ggagccaggg ccgaaccgta ttcggacaca gccagcgcgc ccgcgttgca
    26341 gtgcgtcagc accgtctgtc cgtcccggat cagcttgagg ccatgctcgc cgatggctct
    26401 gcacatggcc gcatcctcgt cgcgtatggc caccgcttcg cgaaccagcc gctccttgaa
    26461 cgcggcgggc ggcagcgaag cgctggcccg cgcgacgctc ttcattcttt ccagcgcctg
    26521 gaaaagattc acggcggtcg ggcgcgaggt ggccagatag tcggcatgct tttccgcctc
    26581 ggccagaagc cgctccccgg aggcttcaga gccctgcagc gcggccgcca cccccatggc
    26641 ggcggcaatg ccgatggccg gggcgccgcg cacacgaagc attttgatag cctcccatac
    26701 gatctctata tccgtcgtgt tcagataggc cagttcttcg ggaagccgcg tctgatcaat
    26761 catgcggata aatccgggca tcatgccttc ggcagattcg gaaatccatt ctatggaaag
    26821 caccttcatg tttatttccc ggctccgctc tgcagcacca ccagcatggc ggtgttgacc
    26881 ccgttgaaca ccgcgtgcat gacaacgggc accagcaggt tgcccgtgcg aatataggcc
    26941 agcgagaatg ccgcggcgat gagaaacagc gggatggtct ggaaaagata gccgtgcatg
    27001 gcggcgaaaa ccagcgagac cagcaccacc gccgccggca tgcctgtacg gcgcgcgagc
    27061 catggcaggg caatcccgcg gaagagcacc tcctcgaata caggcgccag caccaccgcc
    27121 agcaggatca gataaatccg tatccaccag acatgggccc cggcgacgat cccggcgatg
    27181 tcctgcagct gcggctcaag cccgaggcgc accagcacaa actcggccag cacggctccg
    27241 gtcagcagca cgggaagcga ggcgaggtaa tagtagaacc cccgcgccgc cgaacgggcg
    27301 gccccgggcc agtccaggcc gaaggcggtc tttccggtcg ccccgcgctg cagcagcatc
    27361 gcggcggcga gtgccaggcc cgcccagtgg aaaataaggc tctgcaccaa aacgatcatc
    27421 ggcgatccgt cgccgctgac ggcgtcatgc gcgtaaaggg actgcaccat catcatgccc
    27481 gccagatgca ggaaaaccag ccagagcacc agtcgccgga aatcctctga aggccaggcg
    27541 cgggccttga gccgttcggc gcaggccgcc cacgaaaagg aagcgccgcg gaaattcgaa
    27601 agcagcagcg cgtcagctcc cagtccggcc agaagcagga agaaatagaa aataccggcc
    27661 atccatgcat gcggaagctg ggccagtgcc tccggattga gttcgagcgg gatttccgcg
    27721 agaaacagca tgagaaaaca acccatgaga cggaatccta cacggcagat tccgccgctt
    27781 cacgaatcac ataaataggc ttgtcctgcg attcatggta ggtgcggatc tgcatttcgg
    27841 ccagcagccc cagaccaatg aactgaatgc agaacgtcag cagcatcagt ggggtcagca
    27901 gccagaatgg acgcttgagc agatagggat cgttccaccc gaagatgcgg tcggcggcga
    27961 cgacggccat catcagcagc gccgggacgg cgaacaggcc gccgattttc ccgaagatct
    28021 gcatcggccc catgctgtag cggagcagaa acttgacggt gatcaggtca agaacaacgc
    28081 ggaacgtccg cgaaatcccg tacttagatt tcccaaactg gcgggcgtga tgattgacca
    28141 ccacctcctt gatgtccccg cccacccagc tggccagcgc agggataaac cggtgcattt
    28201 ccccgtaaag atgaaccttt tcgatcacct cccggcggta ggccttcagc gtgcacccgt
    28261 aatcgtgcag cgccacgccc gtgatgcggc tgatgagcga gttggccagc atggacggaa
    28321 ggcggcggtt gatgaacgga tccttgcggt ccttgcgcca gccgctcacc acgtccacat
    28381 tgtcatccat gttctcaagc agtttgggga tgtcggcggg atcgttctgg agatccgcat
    28441 ccagcgttat cacgatcctg ccgcgcgatt tgtgaaagcc ggcgctcatg gcggccgtct
    28501 gcccgaaatt ccggcggaag cggatgatcc gcagattggg gattttcccg gtccattctt
    28561 tcagccgggc ccacgtgccg tcgcggctgc cgtcgtccac cagaatgatt tcatagcttc
    28621 ggcccagatt cgaaagcgac tcgtgcagct tcgtgcagag cgcgtccacg cattcttcct
    28681 cgttgtaaac cggaaccacc actgaaacat caatcatcat caaccccatt tcagaaaccg
    28741 gcctgcggcc agcgtgtttt accggcatat tggagaggag gacccccaaa atcaaacaat
    28801 taaacagcgg attctttttg tttggctccc gaaggctttg cgatatactg tccgcagaga
    28861 gacgtatcaa cagaaagggt tgcggggcag ttccatgaac aggaaatcag cagaccagac
    28921 cagaagatgc acctttcgga tcagcacgga aacgccgctc ccggcgggcg agcaggtttt
    28981 catttccgga aacgacccgg ccctcgggga ctgggcgccg gacggcctgc cgctgacgcg
    29041 cgtgcaggac tgcctctgga ccggaacagc cgtgctgccc gccgggcgca ccattgagta
    29101 taaaatcacc cgcgggagct ggcaggccga agaagcgctc ccctccgggg ctttctcgcc
    29161 caacaaaacg ctccggcccg gagccgactc cgtcattgaa aacaaagtcc tccgctggcg
    29221 cgacagccat ctccccccgg cgccgcagat cacaggcaac tatgaaatcc acaggaatgt
    29281 gaaatcgaaa tttctcgcag agccccgtga tgtgatcgtg tggctcccgc cctcctggag
    29341 caaaaagggc gccgcctctt atcccgtgct ctacatgcac gacgggcagc aggtcttcga
    29401 cccgaacacc tccacatgga atcatgcgct ggatgtggat gattactgca cggagctcat
    29461 gaaggccggc gagatgcagg aaatcatcgt cgtcgccgtt tattccaccg gcgaccggtc
    29521 ggtggaatat gatccggctg caaaaggcgg cgactacgcg aagttcattg tggaggaact
    29581 caagcccttc atggacaaaa cctaccggac aaagcccggc cgcgaaagca cggccgtggc
    29641 cggcgcttcg cgcgggggcg acatttcgtt ccacctggcg tggacgcgac cggatgtgtt
    29701 tttcggcgcg gcctgccttt ccccggcctt ccgcttcgag gggcgcgaac cctgtctgga
    29761 cgccgtgcgc aacgcgctga ccgttcccga catcaaatgc tatctctact gcggcagcgg
    29821 agacccgctc gagcgcgagc tgatgcccgg catgcgcgac atggcccgcc agctgcgcct
    29881 cgcaggttac aaggacggag tcaatctgct cgtggaggaa gatgagccgg cggcgcacaa
    29941 cgaggaatcc tggtcccggc acaccccctc atggctgaaa ttcctgttcg gaattcagaa
    30001 tccgaagccg gcccgcaaag ccaggaaaaa ccggaaatag gaagtcagcc gataaatcgc
    30061 gcgtattccg gatggcgggt tttgattctt tcactcgcgc ttttatcgtc gctcaggaac
    30121 gagaaatccc tgaaatcaaa gcccggggcg accgtgcatc cgatcaggct gaactcccct
    30181 gacgtctccg cggcctgata tatgccggcc cccatgatgc agacgaaacc gtcgccagcc
    30241 ccgctgatcc gctcttcgcg gatggtttcg ccgtcatact tgatgagccg cagcgggtcc
    30301 ccttcataga agttccagat ttcgtcatga agcacccggt ggaatctgct gatatcgccc
    30361 ctggtcagca gataatagat gtgcgtggcc gcgtcgcgct ccgcgccggc ggcagcggat
    30421 gacaccttca gccccgacct gaacacctcg cggtaatacc cgccctccgg atgagggagc
    30481 agcttatact tgtccaccag tttctgcatc gtcactcctt gtgtctgtct gagtgcgccc
    30541 attttacata aactgccgca tgccgcacaa tccataaagc ttcggcgggg cacggcagtg
    30601 ttgcagaccg gttttctttt gatagactgc gcgcatgaat ttttatctgg ttttcattgt
    30661 tggaattctt gtcgcacatt atctgctgga tctgctggcc gacgtcctga atgtccgcaa
    30721 ttgctcggag cagcttccgg aggaattccg cgatgtctat gacgccggaa aatatgcccg
    30781 ttcccagcag tatctgaagg accacacgcg gatggacacg gtcgtgaatt cagcccatac
    30841 gctgatcatc ctcgcgttca tcctcctcgg cggattccgg ctcgtggagc ttgccgcccg
    30901 caaagccggc tgggacatga tcggaaccgg actcctgttc ggcggcatcc tgatgattct
    30961 ttcgcagctc ctgcatctcc cctcctccat ctacagcacc ttcgtcctcg aggcgaaata
    31021 cgacttcaac aaaaccactc cgaagacctt cgtcctcgac atcgtcaaat cgctggcgct
    31081 gaccgtcatc atcggcgcgc cggttttcgc gctgatcctc tggttctttg acgctgccgg
    31141 cgaatgggcc tggctctggt gctggctggc ggtcatgctg atccaggcgc tcctgatgtt
    31201 catcgcgccg gtcgtgatta tgccgctgtt caacaagttt gagccgctgg aggacggtga
    31261 actcaaaacc gccatcgaaa attatgcgcg cgggcagaat ttcaagatga agggcgtttt
    31321 caaaatggac ggctcaaagc ggtcggccaa aacgaacgcc ttcttcaccg gcttcgggaa
    31381 aagccggaga attgtcctct acgacacgct gatcgcaaag cactccgttg acgaactggt
    31441 ggccatcgtc gcgcatgaga tgggacacta caaaaagaag cacatcctct ccgccatgtt
    31501 ccggaagttc ctgcaaacgg cgtttatgtt ctggcttctg tcgctgttca tcggcagcga
    31561 acagcttttc ctcgccttca aaatgcccct gcccccgact gtctacggga gcctgatctt
    31621 cttcggattc ctgtacacgc ccatcggaat cctgatcagc atcatcgaaa acgccatctc
    31681 ccggaaacat gaatacgccg ccgaccgcta tgcggccgaa accaccggca aggccgacgc
    31741 catgatctcg gggctgaaaa agctcagcgc ggacaatctt tccaacctca ctccgcaccc
    31801 gttcatggtc tggctcagct acagccatcc gccggtcctc gagcgtatcc gcgccctgat
    31861 ggaggcctga acatgcataa gagaaaaata tccgccggag tggttcatga tgtccctgcg
    31921 gacctgggcg cggcgctcct tgcggacagc gatgctttat ccttgtggga gaatatcact
    31981 ccactcgccc gcaatgagtg gatctgctgg gtcgaggacg ccaggaaaaa ccgaacacgc
    32041 ctcaaacgaa tagagagggt gcaaacagaa ctcaaagcag gcaagcgccg gccgtgctgc
    32101 tggtccggat gtccgcaccg gagcaaaaca cgctggccct gaaagcgcag ccgatgcaca
    32161 ggcgcgcgaa tcagaatttg cagtccagcg ccacaaccaa gttccggccc ggttcattca
    32221 ggcctgaccc gtgaatgcga tagtccttgt cggcgatgtt ttccagcgcc gcggacattg
    32281 tcaggttctt tgtgaaatgc catccgccgc ggacggtgaa cacaccatag cccggcgtcc
    32341 cgccgggcgg aatcctctgc gtgtcggcga catcgctggt tgaaagttta tcctgccggt
    32401 cagccgccgt gaccacggct tcaagccagt attttcccga aggctgaaag cgaatccccg
    32461 cgttggccgt caggggcatg aggcggctga ccggctccct cttcttttcg ggcaatgatg
    32521 tcggatacgt gtccacttcg ccttccatcc atgcgcagtt gccccacagg gtccagactt
    32581 ccgcaaaggt cctgcgtgcg ctgagctcga tgccgtgaat gaaaccgtcg ccggcgtttt
    32641 tcttcaccac ttcgctgtat tcatctatga cgcggccggt gggtgttcgg acgatcatgt
    32701 ccttcatgtc cgtgtagtag taaaccagtt caaaggaggc tttgtcgctt ccgcccctga
    32761 tgccggcttc gtacgccaga tatgtttcgg gatcaagccc cggagaaggg acttccagtt
    32821 catcggtccg ggctgtgtcc agccgcgtga ggtcggaaag attcggcgca cggaatcctt
    32881 gcgaaacgcc cgcgaacaga ttcagtccgc catccggaag aagcctgtag agcagccgca
    32941 cgctgcccac cgcctcgtcc cagtcttctt tcagcgagcc ggggccgtct gcggcagtat
    33001 cctcgtagcg gcccacatcc gcctgcgcat agttgtagcg cgccccggca atcagatcca
    33061 gacgatccga cagcggcaca acatcctgaa cataggcgcc gaccgtttcg tacacggcat
    33121 cgtcccccac cgggccctga atgccaatgc tcttgagaga gccgtccgcc ttgaattttt
    33181 tgagatacga atccacgctg tcgcggtagt actccgcgcc ataagtccac tcacccgcct
    33241 ttgtcggact gatcgcctga agcgaaacgc cgaatgtgtc aacatcatag ccctgaatat
    33301 cgctcgtatc gtccttctta atgcggaact gatcctcctc ctggcgctga tgcgaaacgg
    33361 tgaggttcag atcgcggatg aaaccggaca aatccgcggc atcgaatgcg atgtatgaga
    33421 ggtgccgttc ctggtccaga acgcgctttt tctcatcgcc aacagtggtg ccgtggaagc
    33481 tttccccgta aatcgttttg tgcgtgcgcc aggcatcatc ctgctccact gtctgatgag
    33541 ccgcggaaag ccgcaggttg ggattgatca ggtattccgc cctgatgtcg aagtcaacct
    33601 catcataacc cgtcttgggc tgatcccccc cgccggagcg gaggtcccca aaatccctca
    33661 cagtggctcc agcccgaaga ccggccttgc cgccagccgt tcctcgtcct tctgcgcgca
    33721 cggtgtgaga atcttccgcc gtgctgtagc ggtagtatgc gcggccggaa acggcgcggt
    33781 ccgcttcatc atcaaataca ctgcgcgtca gcgcgttcac ggttccgccc accgcatcgc
    33841 ttccatacaa gaccgatccg gggcccttca cgatttccat ggattcaatg ccgaacggat
    33901 ccacggtgtt ccagtactga ttgggcccgt cacggaatgt ggaattgttc agccggattc
    33961 catcaatcag gaacagtgtg tggaagccgg tgaatccgcg aatgaaaggc gaaccctgac
    34021 catgacccgt tttctgcacc atgacagacg gaatctccct gagcgcctca gggctcgtgc
    34081 gtgagagttt ctccatctgc atgacttcgg cactgaccag acaggtcgtg aaaggaagag
    34141 ctgccgcctc cttctccgac cgagtggcag tcaccatcac atcgggaagc tcgacgggaa
    34201 gtgcagcctt ttcctccgcc tggactgccc cggcagccag cagcatcgca atcaggatat
    34261 tccttgtcat gatcatactc ctcagtctca ttggttatta atccttctaa atttccggtt
    34321 cttctctgtt aacgcaccgg aggaacattt ggttcactgg aaattctgtt ttttgaggga
    34381 cgggatatct gctatacagg atatccatga ttcgaaaaca gaaaaaaccc gatcccgccc
    34441 tgcttgcaga cgcggaagtg atgaagcgtg tccgcgcagg ccatacagag gccttttcag
    34501 tcatcatcct cagatatcag aattctctgc tgaacttttt ccggcggctg ggcgtttata
    34561 cagatgcgga agatctggtg caggaatgtt tcctgcggat attccggaac cggatgaact
    34621 accgggaaac ggccaagttc acgacgttcc tgtacagggt tggaaggagc gtctggatag
    34681 actgggtccg gcggcaggcg cggcgaagca gtttcctgac cgaatacgaa gacggaatgc
    34741 cggaactgaa cgaatattgc gaacgcccgg cgggaagcgg aatggatgcg cagatcgcgc
    34801 tggaatcgct gcccgaaaaa ctccggctgg tggtggtcat gagcatttat cagggaatga
    34861 agtatgcgga gattgcggag gcactcgaca tcccggttgg gacagtgaaa tcccggatgt
    34921 ccatcgcctt tgacctgctg agagattata tgtatgcgga cgccggggag aagaaagtct
    34981 gaaaatgaag cagccctttg aagaattaat tctgccctac gttctggagg aactgcctcc
    35041 cgaaaaacgg caggctttcg aaaaggagat ggaatgcaat tctctcctca gggttcgcgt
    35101 ctatgagaca cgggaaatgc tgaaaaagct gcgggagctc ccgcaggaca ctgtcagccg
    35161 cgacctgagc gctgaaatcc tggaggccat caaatctgag aaaaaacagg gaaacccgtc
    35221 ctggcttccc cgcttgctga aagcggccgc ctgcctggct gtgctggccg gggcgtggct
    35281 tgtgattcag tccatgacgg gcggaaggga agaccccgcc gccgcgcagg gtttcaaatg
    35341 gatcgccgcc gcgcaggaaa cctccggcgc atgggacggg gccaggtggg gcggaagcgg
    35401 aaaacacacc atcgaactga ccagtctggc gctgctgacc ctggcttcgg gaaaatcggc
    35461 aggcatgaat tcggattatg acgagcagat ccgccgggcc gccgcctatc tggctgcgca
    35521 tcaggaccgg gagggaatca tcggagaagg cggcatttgt tcgggctacg gacatgccat
    35581 tgctacgtgc gccctgctcg aggtgtaccg gcacaacccc tccggcgggc tgaaacgcgt
    35641 actggatgga gccatacgcc atctgcagac cagtcatgcc gcatcaggag gatgggcata
    35701 ccagaccagc agcgggaaat ccgtccatcc gaccggttcg gcctgggcgc ttctggccct
    35761 taaacgcgcc gcggcgcagg gctggccgtg cagcggcccg cccgcccgcc tgccctcaca
    35821 gccggcaagc agaatcgacc tttatccatg gtatcttctg agtaccgagt tcgccggcgc
    35881 ggaccggtca aacgccgttc gcatgctgga actgatgaaa gagaatcctg catctgatgg
    35941 atgcctgtcg gccggcggac aaatctattc caccgtcctc gcgacgctca ctctgtgcgc
    36001 cgggaatccg cagaactgaa agttccaatc cctggaagtt tctcaggaaa cagtttcaac
    36061 cattggaagt attttgacga aaaggtccaa tgactggaaa ttccagccgg ccgtttcccc
    36121 tcatccgccc gctctcatgc acccgcgaca caaggtcgcg ggggttttca tttttcgggt
    36181 ttgatgattt tggctccgcc caaatccccg agggttgcct tctccaggtc ggtccagagc
    36241 gtgccttcgc ccgaatgcag gatgttgatt ttgacatccg ggttcaggct ggcggccatg
    36301 ttcaacatgg tgaaggccac aggatcgccg caggccttcg ccttgagctg aagtcccttt
    36361 gctttttccc catcgaccat tttgatggct tcggcctcgg cggtgccgat cttgcggacg
    36421 atatcggcgc ggaccagcgc cgtctgcttg tcaatttcgg ccactgattt tgacgcctcg
    36481 gcgcgtatgg tagccacctg cttctgctgg tcggccttga tctccgcctt gatcttttcg
    36541 gtttcgcggg ccacctgctc gcggcgctgc tcaatcagcg tcagttccgt gttcagctcg
    36601 gcctgttttt tggccgtgtt ctgcttttcc ttgttggtca gatcctgctg cacggcaatg
    36661 ctggcgagct ggatcggctc aagaatctgc atggggacct ccacgtgccg gatgagggcg
    36721 ttgtggacaa tcaggttctt ggccttcagc gtctttgcca gcgcggcggt caggtcgtcc
    36781 tggaacttct ggcggccctc gcccacaatg aaatccttcg cgccgtattc agaccccttg
    36841 ttgcgcgaga tggaagtgat ctgcgggagg atgatcttgt ccaccaccgc cggcaggtcg
    36901 ccgtagcggc tgtagatgaa ggcaatgttt tccggcagca gttccacctc aacggtcatg
    36961 tcgaggctga tctggaatcc gtcgcgcgac gggaactcaa caaactcagt gagtcccatc
    37021 atggccgttg tgtccgccat ctgctgctgc ggaatccgtg catcgccata agcctgctcc
    37081 tgcatggccg ccggcgccat cgccctgcgg gcgcgcacgg agctggccac ctgagagagg
    37141 tagtcgtagc gcttctgctg ctgcttttcg agcaccttgg tctggagctt ttccatggcc
    37201 acgttctgca tctcgatctt gccttttgtg atgacctcgc cgcccatctt cccgaccagt
    37261 gaaacctggt tcacgccgat ctccagcacg tccaccttga actccttcgg atttacgtag
    37321 tagaggccgg gctgcaggat atcctcgcgc acgcctttct ggttcagttt tgcgaacttt
    37381 ccttccgggg cctgctcgcc agaaagcgac gtgaccacgc ccgcatagcc gaccgggatg
    37441 ctgatggcgt ccaggatatc gatctgatac ccgtagggat tgaaacggta cttgccgggg
    37501 cccagcacgc cgcgccagat gcctttctga tttctggccg cgatgaactc gccctgcggg
    37561 agttcttcgc cgatcttgga ggtgaccagc ccgacctggc cgggcgggat gcgctccacc
    37621 gaacggattt catgctcata gagaatggga ttcaggaaat ggcgtccctc gccaagaacc
    37681 tcctcgcgca cgcccttctg gcccttgccg gccagaatct gtccgggcgg aagatctttg
    37741 ccgaccttcg ccgtgatgac cgccatggag tccggctcga catagaagcg gcagaagccc
    37801 cacatccaga cgccccacgc gacaaccaga acaagaacca gagtgctgat ggatctgatc
    37861 atatctttca ttgggcgcct cctttccctt ccggcagcag cgggccgaag atggcgccca
    37921 tcccctgctt ctgatcgctg ctgagcacag agccgatctg cggcgcgatt ttttcataaa
    37981 agcggtagcg ggccaggttc atgccgtttc cgaatgcgct gacctgtgaa gccagaacgg
    38041 aggcttcggc caggttccgg gcttcaatgg cgtccttgtc tccggcggcg cgggccagca
    38101 ccgcctcggc gcggaaggcg gcggcctcat tgtcgagttt tgccacatcc agctcctgct
    38161 gcgcggcaat gatgcgcacc tgctggtcct gccgggcatt gatcagagcc gcaatgcggg
    38221 cggtgtccgc ttcaaccttt tccttgttct gcacggccag catttcctgt ttcacgagtt
    38281 cggcctgcga cttggcctgc tcgatctgct ggtcgaactt gagggcgttc tgaacggcga
    38341 tttcgcgttc gcggatgatg ctcgcgattt catccgggac aataatcttg cggatcagca
    38401 cggaattgat gctcacgccc cagtccatgc acttctcgcg cagatgagtc tccaggtcgt
    38461 tctggaactt ctggcgggtt tcgccgacga tgaaattgat cgccgggtgc ttgctgccct
    38521 cgatgcggct gaatccgcgc gcgcgcggca gaatgacctt cttgatgatg tcatccatgt
    38581 cgcccacgcg gtgcgtcagc agcgcggcct gatcgcgctg gaggacatac tcaatggttc
    38641 cctcgacggt gacggtgaat ccgtcgaagg tcaggaaagt aatcacgtcg gcgccgctca
    38701 tctcgaagcg ctggctctgg agattcacct cgaccacgct gaactcatag gggttgagat
    38761 aatatgtgcc cggatccagc accgccgtca gcaccccctt cagcccctcc ttcacgagga
    38821 acgtgttgcg gtcaggctcc ggaagttcgg catccagcac gtcgtccccg atcagagcgg
    38881 tcacaacgcc cacatgaccc ggacggatgg tgatggcatc ggccagttca atggtgtagg
    38941 catacggatt gatgcggtat ttgccggggc ggagaacttc acggaccaca cccttgaacc
    39001 cgtcgggagc aatgatcttg ccgaccggaa gagcctcgcc gtacaggcgg gttttcacgc
    39061 ccagcttgcc ggccggaata tccagaatgc tgtggatttt ccatccccat gtatagggat
    39121 tgcggaaata gcggccttcg ggaagcactt caagctggat gcccttctct ttcgggttca
    39181 tggcgagaat ctgaccggag ggcaggtctt ttccggtttt ccggatcagc accgcgattt
    39241 cgccggagcg cggctcgatg cggcatccga accagatgaa cagcggcagg gcgatcagca
    39301 gaaagactgc gatcaccgga atccaggaaa tcgctgacag atcggggatt cggggtttct
    39361 tcggcatggg gcccggtcgg cggggctcgg agtgttcgat gtcattcata cgcatcctcc
    39421 tgatgaaact catgtgcgga aagcatacgg cacaaaggcc cctgccgcaa gagggaactc
    39481 gtcaatccgg ccctggcctt cgcgcgggga attcaggggg caacacccgc tttctcccat
    39541 gctgcaacgg catctttaac ccccttcgcc cgggccagcg ccggaggcgt cagtcccctc
    39601 gcattcatta tccggggatc agcgccatgc tggagcagaa gcggaatccc cttttccggt
    39661 ttccgcccag ccgatgccag atgcagcggt gtgctgcctt cgcgattcct gatgttcggg
    39721 tcggctttcg ccttcaaaag caccgcagcc agatcccatt tgccgtaagc cgccgccaga
    39781 tgcagggcgg tatccccatt tcgatcctgc gcattgaccg gagcccctcc ggcaatgagg
    39841 gcctccactt tcagcacgga gagcacggcc atggccgtgt gcagcggggt ccgtcccgcc
    39901 gcgttggtct tcttcggatc ggcccctccc tcaagcagaa gcctctcaat gtcgtcgcgg
    39961 tttatgccat cgccccaaat cgcccggtgc agcggagtgt tgcccgcctc gtcttgttca
    40021 ttcaccgctg ctccgcgggc tatcaaatgc tgcaccaaat gcagatcatg ttttcgcgcg
    40081 gcctccagca gagtgggttc gtccagtccc ggcgcacgcg caggctttcc cggctttttt
    40141 gcaggattca gatcggatgg cagccggccg tcgaaatctc gtatggcctg atttgcaccc
    40201 gaagccgtca gccggctgac cagggcagca tagccttccg caaccgcgta atgaagcgcg
    40261 gtgcgtcccg tgcgatcctc aacgttcaca tccgcgccgc gctggatcag gatttcggct
    40321 gatttctcag gatgaggggc ccttttctgc gccagacaaa tgagcggcgt ttcgccggca
    40381 tcattcgctg cattgacatc acacaccgcc gccagcattc ggaccacctc tgtcttttca
    40441 tcctgccggc cggcataaac ggcggtgacc gccgcggcgg cctcatgcag cggagttgaa
    40501 ccatcggagt ctggagcatg cgggtcagcc ccgttttcca gcagaagcct gacgatcgcc
    40561 ggatgggaaa agcgcgccgc cacatgcatc ggagttcttt gaatccatgc gtcagaatga
    40621 attccgtatc cgcggccgta aagattgtgc ttcgcacctt tatcaaggag cagcgccgcc
    40681 acatccgccg cgttctgctc agcggcataa tgcagggggg tgcggcctga aatatcctga
    40741 gcattcacat ccagtccggc tccgatcagc gcttcgacgg ccgacagcga tccggttgct
    40801 gccgcataat gcagcgcggt cccgtttccc ctgctcagga tgccgggata agcgtcacgc
    40861 tcaagaagga accggatcat ctccgcatgg ccgccctcgg ccgccaggtg cagcggggcc
    40921 tcgttttcat agctgcgcgc attgatatcc gccccggctg acagaagaat ttctgccacc
    40981 tccacggagc ccgcgcgggc ggccaaatgc agaggcattc tttcgtagtc ccccctggca
    41041 tgcacattgg cgcctttctc cagcagcaga tggaccacgt cggcctgtcc ggccgcagca
    41101 gccgcatgca gaggctggtt acccttcgcg tcaacggcgg cgatatccgc tcctttatca
    41161 ataagcgaag ccgccagctt tacatttccg gcggcacaaa cttgatgcag tgtggcactt
    41221 tttcctgctg cgggagcatc cggtttctct ggagccgcag ggcggtccgt ttcctgccgt
    41281 gcggcgcggg acggggtcct gcgctgaacg ggctttgccc cgcccgccgg attgaaatcc
    41341 ggcgcgatgc gttttgcgtc atccggcttc agatccgaca aactgtgata gcttgtgctc
    41401 ttgccggagc gcacctcaac aatggacttt ccgacccggc tgacttctcc cgtcagcgtt
    41461 ttccccgtct tgagggtgac cacctctgcg cgacccgccg ttgcggccag catccccagc
    41521 atgattgcaa ctgttggttt cataaccggt tctcccgtaa tcaaaggttt gtgctagccg
    41581 tggcgaaagg aattccaggt tgatcggata agccagaagc ccacgagggc aaaaacgacc
    41641 accgcgccgg ccatccagat cataccggga tggatgaacc actgctcctc gttcaaatcc
    41701 agccactccg gatggttggc ccgaatccat tccatgcggc ggcggtgatc atgtccgaac
    41761 tgtgttccgg cgattgcatc cagcgcatcc gcggcataat tcctgcattc cagatatgca
    41821 tcaccgttca tgatcagcat cagaaatgtc accgcccgag ggtctttgat ctgccccaga
    41881 gccttgatgg cggcataccg gttgttgaga tatttctcag aatgcgaggc agcggccatg
    41941 agcgcatcca ccgcccgggt ttctccgagg cggcccagcg cctcggccga cgcttttcga
    42001 actttgtcgt cgggcgaagt ctgcagaaga cggatcaggg gctcacccac gcgcgcatcg
    42061 cccatcttcc cgagaactga aattgcggct tcggcattcc cctctcggcg gccgtcggcc
    42121 atttcaagca gaaaaggaac cgccctgctc ccgatctgca aaatgtcccg ctgcgcatca
    42181 cgcgccactt tccagtcttt gtcggaaaga gatttcagca gatacttcag agaggggtcc
    42241 ggttcggcca gggcgggctc ttccgccggg atgcatgcgg caaaggacat gacaagcagt
    42301 gcagacaaca gcagtttttt gatggacatc gttccctcct gtgatttcgg cgctgtggat
    42361 tcatactatc cgacgccggc ggggtattac aagctcccat ccatttgaac ttttcacagc
    42421 tttattctcg cttcctccgg aatggatgca gataatgaag gccgtcggat ccaaatgcgc
    42481 accatgcaga acagcgggag gcggaagatg acatatcgca aagcgggaat tgtttttctc
    42541 atcacggttc tggcattcag tcactgtccc ggacaggatc ccgcacccgc cccaggcgcg
    42601 cccgtgaaga tgtccccgga ggcggccgtg cttcagaaac tcctgaccgc gggccagatc
    42661 aaaccgggga tcgattacat gaggaagaag ctggccgaag atccggaaaa cgaccggctg
    42721 agatacgcgc tgggcctgct tcagttcacc cgcgcggttg aacggctgtc gcagtcatgg
    42781 taccggtacg gcctgcggcc gccgccggaa ttcgcacggg aactgccttt tctgcgcctg
    42841 ccggtcgcat tgaatccctc tccggaagag gctgatcacg ccgcaatccg cggagtgctc
    42901 cagacttttc tggatgatct ggcccgctgc cgggccacgc tcggcgggat gaaggacgaa
    42961 tcggcatcca tgccggtccg gctgggactg gtccggctgg atctggatgg tgacggccaa
    43021 gccggtgacg gggagatgct ctggaacatt gtttcgagga tcatgcaggc cgaccttccg
    43081 ccggaaacgg tggagagctt tgtgatcgag tttgacgcgg ccgatgcgta ctggctgcgg
    43141 ggctattccc acattatgag cgcgctgctc gaattctggc tggcgcacga tgacgcagag
    43201 ctgttttcag caacggctca tctgtttttc gagcgaccga atccggcaca tgatttcctg
    43261 ctggaggagc gcgccgatgc ccgcgcgccc ggaaggaggg tggtgccccg tcacttcttc
    43321 gatgcggcag cgctggtgca cgggatgatc agtctgcccc tgaaggaacc cgcgcgcatg
    43381 cacgccgtgc ttgaacacct gaggcaggtg accgccatga gccgcgaatc gtggcgctgc
    43441 tgcatggctg aaaccgacaa cgccaatgaa tggattccca atccaaaaca gagcagcgtg
    43501 attcccggcg cgaaagtgac gccggaaatg atccgccagt ggatggccat gctggatgag
    43561 ctggacgcca tcctgaaggg agagaaactg attgcgttct ggcggggcag tcagccgaga
    43621 ggcgttaaca tcaaaaaggt attcgagaat ccggccgact ttgatctgat cctttggatt
    43681 cagggaactg ccgccgagcc ctatctggaa tatgggccgc tgaccgatcc gtcattctgg
    43741 aggcagctga ccgaatcatt caacggacga tttgtcccct acgccatctg gttcaactga
    43801 gcgggagcgc ttctcttttg ccgcctgttc cgcggcctgt ctgtagtccg gatcaagcgc
    43861 ctcgttcagg agatgcgcca gccgcacacc agccttctga agctgttcac gaaccacagg
    43921 gacgcgggca ttgatataat tatccagcgt cacatccata cccggacgcg accatttgaa
    43981 gggcaccggc ttgttgcggt cagcccagcg ataggccagc ttgcgcgctt tccagtaact
    44041 gtcaatggcc caggcgggaa tatcatcatg ccgccagcgt ctcagctgtt cgggagtgat
    44101 ttcctttctg agccgcttgg caaacgcaaa cggcgtcagg ccttccatgg cctcgttcac
    44161 cagcgcttca tcccaaatgc tgtgcagact gggagcatag tccatgggcg tgtcggcatc
    44221 gaacgaataa tgctttccct tgaaggagtt caccgggatc atgttgccgc ccatgtctcc
    44281 ataccggtag gcacagtgca tcggctgatg catatctccc gcgaaatgga ccacaaaacg
    44341 cagggcatcc aagcgcttct ccgtggaaac gcggccgtcc gacaacacat ccttccaccg
    44401 gccgagctgg gtgacaatgc tgtcgccatc ttcagggggc tcgagcttga aatcgtcgtt
    44461 gtaccgtttg gcggcatcgt agtccacata atgccatcgg ccgttgcgcg gataaagggc
    44521 ctcgtattcc ttgttgcccc gaataatatc gggccagctg gcgatttcat attcatgcag
    44581 cttgtagctc cccagtattt cctgcagctt ctcgcgggtg gcgggggtca gataatagtc
    44641 ggcaatgcgg gcgatgattt catgcccctc gggcgcccag gcctgaaccg gcgccgaaag
    44701 cccggcccac acaatgcccg ccgccgcaag tttcaacagg gatgatccga tatttttcat
    44761 agtgctcctc tggtaaaaag caaacctacg acatgaactg cgccccgtca acagcaagcg
    44821 gacacgctca ttttgattcg cggaaccgga cccatgcgcc aaccagcgtg gccagaggca
    44881 ggaaacgggt aaacggcatc atgcaatagg cgcccgccag cagggctcca atgatgcgat
    44941 gctgaagcgg aagacggtaa atggagaata tccagaaacc gacaatgatt ccaatgatga
    45001 acgcaaaagg gcccatcgtc gcgaaatcca cgcagtcgag aagcacgcct gccagcacag
    45061 gcccaaatgc gcgttccgcg cgccgaacag gatgcgatgc atccggggtt tccgccgggg
    45121 cggaggaatc ccccgattcc tccgcttccg ggcagacagt ttctcccgac gccatgttac
    45181 gggagcagtt cagcttccag ccggtagaag accctttcgc ggacattggt gtccgtgagc
    45241 gtcatggtcg tattcgtgcc ctgctgatcc tgcaccaccg ggagccaagt cgcatcgccg
    45301 aggtccagtt tgtaatacag cgaatactgc cggccggtcg aggccgggaa tgtcagcgac
    45361 tggttcagca acaccgcact gccgacgccc tgaaagacag atgacggact cgtcgggtct
    45421 gtgtcggcca ggtactcatc atagttgctg accgtgtcat tgtcggcatc ttcctccggc
    45481 gcgaattccg gatcttcggg attctcgccc tggcccgcga tccattcttc ataaggatcg
    45541 gccggcggcg cggattccag cgttgtgacg ctgccggttg gggaatttcc actggtgccg
    45601 acagcgttga ccgcgcgcgc gcggaagtag taggtcgtgc cggcggtcag tccggtcacg
    45661 ctctggctcg ttcccgccac ggtccggtcc tcataacccg gcacaaagct gggtgagctg
    45721 ccgcctccgg cctggcccac gaagaagtta tcccagtaca ccgtggcgcc gctgtaggtg
    45781 cgcacttcga agttcagcgt attggcgcca ttgttaggaa caacggtata ggtcaccttg
    45841 gtccagtccg atgccgtcgg cagataagtg gccggctgaa gactgtcgcc ggagacctgc
    45901 ccccccgatg cccagctggc ccatatgcgg cagttgcctg aaccgggttt cttgtaccag
    45961 aagctgactt catattccgt cacaccgtcc ccggtgatgg caaccggctg catcagatcc
    46021 cgggtccctg ttgccgcgca ggagacatga taggtcccct cctgcggatc ggtcgtcacc
    46081 acggaatatt ccgtttcgaa cttggtccat gccgtgctgt cgccggattc gaatcccggg
    46141 ttggtcatca gattggaacc gccgccgcca ccgctggaga atgtcgcgct ttcgctgaca
    46201 tccaggcgat aagaggccgc gcccgaagct gtgctccatg ccgcggtgaa ctgctgcatg
    46261 tttgtcgcgc tcgcccagat ggatgccggc gcggacgggg ccgagggggc ctcgctcacg
    46321 accacgctgg tgatctgggt tacagttccc gcggcgttgg ccgctgtaaa ggtgaaggtc
    46381 tgcgtgccaa cttcgcctgc cgtcggcaga tagaggaaat agcccgtgcc cgtgtcaaag
    46441 tcgtaattgt ccgcggaggc ggtggaactc tgcagggcca gcgccggcgc cgtacccgaa
    46501 gccgtcactg tgaacgccac ttccacaccc gtcgtcgtgc tgtagggacc ggcccctccg
    46561 gtgaacaccg gcgcggtggc cgccgccacg ttcacggtca cggtctggta ggccgtgccc
    46621 tggctgttga cggccgagaa acggaaggtc cgcgaaccgg catccgactg cggggcgata
    46681 tacagcaggt cgcctgtgcc cgtgtcaaag tcatagctgt caacggccgc ggttgtttca
    46741 accagcgcca gtgtcggagc aggcacaccc gaggcgctga ccgtgaattc cgtgtccagt
    46801 cccgccgtgg tggccacggg gccggggttc gcgccgaaca ccggcacgga caatgtcgca
    46861 taggtcgtca cattcgcgac cgatgagttg ggactggttc cgccgccgga caccgcccgg
    46921 gcgcggaagt agtaagtcat gccagcggtc aggccggtca cactctggct ggtcgaagcc
    46981 acggtccggt ttgagtagcc tgcaatatag aacggattgc ctgcaccacc ctgcactatc
    47041 acatcatcca aagcgcagtt atacgacttt gtgaaataga agccgatttg cgttgtctgc
    47101 ggattcaacg ccacattgta ggttgcgccg ttctgggcta ttaccactgt atccaccagc
    47161 gtccagacac tgttcaccag gccggaaata gcgaacgtgc tgcccgcgcc cccgttgcca
    47221 taggcccaga actgcaaatt cgttgctccc gatgcaaagg tcggggtgat cgcataatct
    47281 cccgtgccgt cgaacttgag ggaataggtt ccgctcagga gtgtgcccac atagggcgaa
    47341 ctggaatagg ttcccagacc ggaaagggtc cagccgctcg gcgaggcgga cgcaaaacct
    47401 tcggccagca gattggtggc ggctccgccg ccgccagttg agaaactgcc gttggtcccc
    47461 acatccagcc ggtagccggt tgcgcccgac acggaactcc aggcagcggt gaaatcggtg
    47521 gtgttcgtcg cgctggccca gatggaggcc ggggccgcgg gcggggcttc atagacggtg
    47581 acgctcacga cctgcgtgac cgcgccggcc gtattggtgg ccgtgaaggt gaaagttctt
    47641 gaacccacgt ccgccagcgg ggcggtgtag ctgagctgcc ctgtcccggc ggtgaagttg
    47701 tacccgctcg acgccgtcgt gctcttcaga gccagtgtgg gagaaggata gccgttggcg
    47761 gtcacagtga acactttggc gacgccggtg gttgcgctga gaggacccgg attggcgccg
    47821 aaggtcggcg gggtcggaga cgacggcgta tactgcatat agcacaccgc gcaatggaca
    47881 acagccgcat cataacggag gcgcatatag ccgctctcgc cccaggatgt gccccagctg
    47941 ttgcgcagga tccaaactcc gccgccgcct tcgggcgggt tgtcatccca gcccacgagc
    48001 gaaatcgcat gattggatgt cgtataccag ccgctcgagc aggagttgtt gctgtcgtca
    48061 tagaccccgt cgtcataggc atcaaaggcc gacccgacat acacagcggc atcgaccacg
    48121 ccataggtca tgatggcggc tttgatggca tcaatgtctc cggaggtcac acgcgcccat
    48181 gcatcaaaca cggtggtggt gccgccgtag gtgcaggatc cgggatcgga cccgttgttg
    48241 tacggcatct ggttttctcc gcagattccc tcgacggtca gcgcctccag ttcggcgtag
    48301 tcataatccg cgccatcgca gccggaaaag tgagaggaat agtccccata ctcacccaga
    48361 caccatgcga tataggcttc cgagaaatcc acgcactggc tgccggtctt gcccatggca
    48421 tagttgaagg ttccttccgc ggcggcggct gcgccaaacg catagcagct cccgcaagaa
    48481 ccctggttgc ggaccgatcc gatatagctg gcgccgccca tgttgcgcca gtcccatgac
    48541 gacggaagct ccatcttcgg gaatgtcttg agcggagccg ttttcattgc agcttcgatt
    48601 tcggccggag tcgcctccat cgcatcgtcc cagagaggct cgccgttctg cttcatcagt
    48661 tcaagaagag gaatctcctc cgcgccgccg gccttcagcg gcgctttcgg aacacacacg
    48721 gcatagctct gcacaaagcc gtctgcctcg acgcgcctgt tgaccgtgtc atagccgaac
    48781 tgcgcgccat aggcatattc actgcccacc ttgccgcgat agaaatccca ggcatccagc
    48841 acgacgccgt cggccacggc aacggtttca ccatcttcgg tggcggaata ttcataacca
    48901 agctttgccg cataggcggc ggcaggattg ggaatggcat gggctgaaag aacagccgca
    48961 aaaaccagca ggaaggacac gagaaaacag ttcgcagttt tattcatcag gcacctcttt
    49021 tttctagtaa aatatttgac cagattatca aatccggtat cggccgcaaa agtcttattg
    49081 gaaaaatatt ttaccggata gcccggccgc cgggtggccg gccgccaaaa aatatcaaaa
    49141 tattcggttt ttattgatat tttggcattt ttgtcaattt tttggcgatt tttgggcatt
    49201 ttgagccatt ttttgatctt ttttgctgtt ttccggccat tttttgagct ttttacggtt
    49261 ctgcataaaa aaagaggcgg aagcccgctg aaggccctcc gcctcatgat tcgactgtca
    49321 gaaccgcatt atggcagcag ttcagcctca aggcgatagt acgcgcgcat tcgcctgttt
    49381 gtatcggtga gcgacatgat cgcgtttgtg ccctgcacag cctccagaag aggaatccac
    49441 gccgcatctc cgatttccga tttccacaaa agggaatagc gccggttggt cgaggccggg
    49501 aaggtcatca cctgatgcag tgtccccgct gcgcccgctg cctcgaatac agaatcagga
    49561 tcagtcgggt cggtatccgc aagatattca tcatagtttt tcatcgtgtc gctgtcatag
    49621 tcgtcgtccg ccccgccaag cggaccctcc gggtccattc cgcgaccatc ggcccagatg
    49681 tcgtacgggg tgtctccgcc ggttcctcca tcaaaagaat ggctgcccag accggagact
    49741 gtgtcccggg cataggaatc ccattctgat gccagcgaag ggaacccggc ggcgggattc
    49801 accgtgatgc ccccggtcac agacgacttg cgaaccagcg tttgattcac cgtggaatgt
    49861 gaaccatccg tccaggccgt gcccggatcc tctcctatcc ggccgaagat gtccgcaaag
    49921 gaggccgatg acaccttgta caatgccagc gcatcgtccc cgttgaaatt catcaccgtg
    49981 ctgtttgtta aatttgccac ggccagaatc gccgcattcg cgcttccgtg cgtgattaca
    50041 taaacggctc cgttggccag cgtactgttc agggaaacgc tgctgtaact cgtgctgccg
    50101 ttgaagtatg cgcggatttc gtagtcgctc aaatcaatgg cggtgccggt cccgttgaag
    50161 atttcaaccg ccttgttata gctttcgcct tcaatatatt ccgaaatgaa cagatcagaa
    50221 gccccgctgg cggcagcgct caccgtaacc gtcatcgcca cggccggact cgtgccgtcc
    50281 ttgtccgagg ccgtgaaact gaacaagttc acccccgtct cgtttgtggt gggaataaag
    50341 agaaaatcgc ccgtcgcggt gtcaaaatcc catgtattgg tgtccaccgc gctcgtgcat
    50401 gcaaaagcaa gaatggggtc accgtcggtc tgagccgctg tcacggtgta ttcgaagtct
    50461 tccccgccca ccatcgcgtt ctgtgaggga atcgcgtcca tcacaggcgg ggtgcccgaa
    50521 acttccgcca tggtcgtcac actggccacg accgagttgg aacccggtcc aaccgcatcc
    50581 actgccctag cgcggaaata ataagtcgtg ttcggggtca ggccggtcac gctctggctt
    50641 gtgcccgaga ccgtgcggtt tgaatagccc gccacataat ccggttcccc tccccctgtg
    50701 ccggcaaccc ccgcgaagaa gctgtcgaaa ctcatgctcg cgccggagta ggtgcggatc
    50761 tcatagaaaa gcacattggc gccgctctgc ggcacgactg aatatgaaat tttagtccag
    50821 gctgatgtgg ccggattgta agaggtcggc tggaggctgt cgcctgaaac ctgcccgccg
    50881 gttgtccatg cggcccagat gcgcgcattg ccggtgccct tgtaccagta gctgatctca
    50941 tattccgtta ctccgtctcc cgtgatgctg acgttctgcg tcagatcgcg cgtggcgctg
    51001 gctatgatgg acacgtgatg cgcgccttcc tgcggatcgg tcgtcacaac ggcatactcg
    51061 gtctcgaact tggtccagcc cgtgctgtct ccggactcaa accccggatt ggtcatcagg
    51121 ttgcttccgc ctccgcctgc gctcatgaat gtttcagaag ttccgacatc caggcggtag
    51181 tcaatcgcat cgggaacgga gttccaggcg gcggtgaagg cggtggtgtt ggtcaggctc
    51241 gcccagatcg tggacggggc ctccagatcc ttctccagtt caaacacctt catcacggcc
    51301 agatgctgca tgttttccgc ggcggagtcc gtcgtgagcg cgggcggcgg cacgcctccg
    51361 ctccaggtca cttcagatgg acgagtatca aacaccatgc cgttggggaa ggtctcgcca
    51421 tagcagacga tggagcagtg cctctcatcc aggctggggc cgggaaggat caggtcatac
    51481 ggcctgtact ttccgctgtt ggtgtccctg tcgccgtcct gatccgcggg ctgatgggcg
    51541 tccgaaacga tgttggtcag agttttcagc gcccattcgg ttctgttggt cagattcaag
    51601 tcgcccccga ccaccacata gccgttcgtc agccatcccg cctgcgcaat gtaattggtc
    51661 agggcgcgcg cttcggcaag gcgggtcgcc gcatcctctt ccggggtttc aaggctgctg
    51721 gccttcaggt gcacactcac ggcatgcaga tctttcgatc ccggcagatc caccttaacc
    51781 catgcaaaat cccgattaaa gacctcgccg tcaggccatt ctccataatc cacaatgggc
    51841 cagcgactaa tgatgccatt gggctgggcg taggtgccgg tctggtattc gacataatat
    51901 gagaacccgg ttccaaaatg ctcgtccaca aacccgcgat aggtgatgcc gggcttgagt
    51961 acccactcct gcatcagcgc cacatcggga cacagcgcct ccagaatccg gccgcctgga
    52021 tcatcccatg ttgtggtgct gttgacctcg ttggtgccgc tcgtgagatt tgccgaaaca
    52081 atggagaaag cgtcgttgtc gcggacaaac agcacagcct gggtggtgct gcccatcgtc
    52141 acccctgaca caggtcccag ccgcagacga gccgattccg ggccctctgc aagagcgtca
    52201 tcggtgatgg taaaaaccat attgctcgct gtggatcctg aagccgagaa aatcagcgtc
    52261 gtggagcagg aaaaatcggt cccgccggga agcgccgtgc ccgcaatggc cacatgcacc
    52321 gtggcattgg ccgaagctga aagaaccacg ggcaatgaaa cggaggcggc gccttcctcg
    52381 ccatatactc ttgcagttcc aaacgacacc gtggccgcat gcagaaccgg ccctgtcaac
    52441 aaagcaagga tcaccccaac aatctgtttc ttcatcaatt cccgtctgtg cggcaaaata
    52501 cacgcacatg atccggccgg tcctgcccgg ccggaagctc tgaccatcaa aatcttcccg
    52561 tccccgctac gggagcagtt ccactttcag gcggtagtac accggatcca gcttgttggt
    52621 gtccgtgatg gacatcagag tgttcgtgcc ggccacctcg gtgacaaccg gagtccagtc
    52681 cggtccgctg atgatgttcg tccgatagta gagcgaatac atgcgggccg tggagccggg
    52741 gaacgacagg acctgattca gcgccaccgc cgcgccaatg cccatgaaca cggaatccgg
    52801 atcggtcgga ttcgtgtcgg caaggtattc ctcatagttt gtgaaatcgt cctcatcatc
    52861 gtctgcgctg gccacgagcc cggtcgaagt gccggagtac tgcgattccc agtcattcgg
    52921 aattccatcg ccatcgtcgt cattcccggc cgacgccacg attccggtga tggaaatatc
    52981 atccacgcac cagtaccagt cgtaggaagc ctggtaatac cagccgaacc ggacctgtga
    53041 atgcccggaa accagcgatg agacattgct cacatacgtc gcggcattgg ccgtgtccgc
    53101 cccgtcccac gtggcaatga acacataact ggtgagcgac ccgtcggtgt tgtaccatat
    53161 tccggcccag tcgtttgtgc tgtaattccg gaaatagtgt tcaaaccgaa taacgacgtc
    53221 cgtcgcgccc gacatatcca gcaccggact caccatatcc gccgcctggg acttgcccgt
    53281 tccgtagtag tcactgtcca tgatcgcgaa accgttccct gcagaagacg cgttgaacat
    53341 gcgggccttc ggattgttga acagccactt ctgttccggg ctgttggcca gccagtctat
    53401 gttggtccag cctgagggca ggccggaact gaagtcgcac accagcagat tggttcccgt
    53461 gcctccgacc ggagccggat tcaccacgat ctgaatggtc gaagtcactc ccccgtcgtt
    53521 atcctccgcc tcgaagaccg ccacataggt tccggtgacg gttggtgtcc acgtgaaatc
    53581 tgacgataca ggcgaagtgt ccaaatcccc cgggaaggag gcccccgaag gcaggcttac
    53641 atccaccagc acaatttcat cgccatccgc atcggtcgcg gtcacagtaa ccgtcagcag
    53701 attactcacg gtcacggaat gattcgtgcc ggccgggctg taggagatca ccggcagatt
    53761 ggtgccgtcc ggagttcctc cgccatcaaa atcgtgatca ccgagatagg acacc
//
LOCUS       DAOQWR010000003        39533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1168_length_39533_cov_6.090962, whole genome shotgun sequence.
ACCESSION   DAOQWR010000003 DAOQWR010000000
VERSION     DAOQWR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 39533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39533
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_39533_cov_6.090962"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..318
                     /locus_tag="PKK36_00485"
     CDS             76..318
                     /locus_tag="PKK36_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009722422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNR93066.1"
                     /translation="MTTLTATEARKQLYTLLDDVSDSHEPVQIAGKRNSAVLISDDDW
                     RAIQETLYLTSISGMRESIVKGLKTKVEACSKELHW"
     gene            315..578
                     /locus_tag="PKK36_00490"
     CDS             315..578
                     /locus_tag="PKK36_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNR93067.1"
                     /translation="MSWSIVYTNQAKRDAKKISRSGLKPQALKLLEILERDPYQNPPA
                     YEKLVGDLHGACSRRINIQHRLVYQVMDDIKTVKVIRMWTHYE"
     gene            734..934
                     /locus_tag="PKK36_00495"
     CDS             734..934
                     /locus_tag="PKK36_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93068.1"
                     /translation="MTTVQEIEKAVQSLPEPELGTFRAWFEDFDANAWDKQFDQDAQS
                     GKLDALADEALRDFKSNRCTRL"
     gene            931..1185
                     /locus_tag="PKK36_00500"
     CDS             931..1185
                     /locus_tag="PKK36_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93069.1"
                     /translation="MNHFASPRFWDHYHSLPAAIRKLADDNFVLLKSNPQHPSLHFKK
                     VGRFHSVRIGRKYRTVGVLRDQDIVWFWIGTHADYDQIIK"
     gene            1343..1600
                     /locus_tag="PKK36_00505"
     CDS             1343..1600
                     /locus_tag="PKK36_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004480826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNR93070.1"
                     /translation="MKTALIHARIEPETKKRAEIVLDRLGMTPTEAIRLFYHQISLRK
                     GLPFSVEIPNELTAHTLHNSSKGKDIQNFENLDDLFASWNK"
     gene            1597..1869
                     /locus_tag="PKK36_00510"
     CDS             1597..1869
                     /locus_tag="PKK36_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNR93071.1"
                     /translation="MKSVSQTNQFAKDIKRMIRRGKDPAKMQTVVRKLANGEVLDRRH
                     RDHSLVGEWDSFRDCHIEPDWVLIYFTDAEKLILQRTGTHSDLFKT"
     gene            2040..2330
                     /locus_tag="PKK36_00515"
     CDS             2040..2330
                     /locus_tag="PKK36_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNR93072.1"
                     /translation="MGRTSFEWDPAKDRQNQKKHGVPFVLAQYAFADPKRVILVDGEH
                     STEEQRFYCLGVVGEGILTVRFTYRNNVIRLFGAGYWRKGKKIYEEQNKIHG"
     gene            2302..2535
                     /locus_tag="PKK36_00520"
     CDS             2302..2535
                     /locus_tag="PKK36_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNR93073.1"
                     /translation="MKNRIKYTDEPMDKVRPVKDFLPSPDQLAFKEEKVKVTISLSRA
                     SLEFFKMQANRHHTAYQKMIRNLLDAYAGQHMK"
     gene            2706..2966
                     /locus_tag="PKK36_00525"
     CDS             2706..2966
                     /locus_tag="PKK36_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNR93074.1"
                     /translation="MSKSAMVRARLEPGLKNHAEHIFHRLGLNATQAITMFYKQVELQ
                     KGLPFNVAIPTAATHNTFKATDEGRDLVICNDADDMFDKLGI"
     gene            2966..3238
                     /locus_tag="PKK36_00530"
     CDS             2966..3238
                     /locus_tag="PKK36_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNR93075.1"
                     /translation="MYIPVYTKQFEKDIKRCKRRGKNLNKFKMIATSLLAGNPLDPIH
                     RDHKLVGNYSGRRDCHIESDWLLIYKIDCDHIIFERTGSHSDLFKK"
     gene            complement(4121..4366)
                     /locus_tag="PKK36_00535"
     CDS             complement(4121..4366)
                     /locus_tag="PKK36_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93076.1"
                     /translation="MENRPVSVGDWMLTYLLMCIPLVNIIMLLVWAFGGGAPESKSNW
                     AKASLIWMVIGIVLTVMLIMAAGGLAAALSASGGSAY"
     gene            complement(4382..5905)
                     /gene="proS"
                     /locus_tag="PKK36_00540"
     CDS             complement(4382..5905)
                     /gene="proS"
                     /locus_tag="PKK36_00540"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNR93077.1"
                     /translation="MAKQEKTAITPRRDEDYPEWYQQVVKAADLAETSPVRGCMVIKP
                     WGYAIWENIQFVLDRMFKETGHRNAYFPLFIPMSFLQKEAEHVEGFAKECAVVTHHRL
                     EADASGVLKPSSPLDEPLVVRPTSETIIGAMFSKWVQSYRDLPLLINQWANVVRWEMR
                     TRLFLRTTEFLWQEGHTAHATQAEAEEETRKMLGVYTRFAQNYMAMPVIPGEKTAGER
                     FPGAVNTYSIEAMMQDRKALQAGTSHFLGQKFAKASDIKFQDENGSEVLAWTTSWGVS
                     TRLVGGLIMTHSDDNGLVLPPRLAPAHVVILPVIHKDADRAAVLEYCDRVASELRAVQ
                     YDGRAVRVEVDARDISGGEKVWSWIKKGIPVRLEIGPRDMASDSVFMGRRDREARDKK
                     GVPRAELVATFATLLDEIQNGLFERAKAFRDENTVKIDKKDDFYAYFTPKNAEKPEIH
                     GGFALSHWSGEEAVENQLREELNVTVRCIPQDAPEEEGTCVISGKPSRRRVIFAKSY"
     gene            complement(6087..8156)
                     /locus_tag="PKK36_00545"
     CDS             complement(6087..8156)
                     /locus_tag="PKK36_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNR93078.1"
                     /translation="MIRRRDIFIRILYSAAISFGVIALIFRLVSASHPGVSAAQVVGA
                     VRNASLAAVGIYLLCQLLQAFLRSVRYGVLLKAGGEQNVPSLFHLYLTTMTRNMLVDM
                     LPARLGELSYIAMLNKGYRVRGEDCVSSLALSMLFDFVALLLIAAVIIALPVLGQSAH
                     QLLLISTGILLVITLAGWMALLYAPGWISAKLRAVRFQRPALNRMFGFLHRLADAFTF
                     TRRSGRLTAVVALSLGVRLVKYAGLYLLFAGIAPAFGGHFAEASPVAVIIALITAEGT
                     ASLALLPTFMSFGTYEAGGLAALTLLGFPADVSIVMMLSMHVLSQFIDYTLGGMALVV
                     FTFSAGSPKTEKEMKTITRRKILIASAAVILLVFAAAAAAFSWRGIKKSGSLTPPKAG
                     HEEGLSREQRNKLRQALRELDGFIVWSSNRGGNHDIWKMDLPSGKIRPLTSHPHTETY
                     PRISPDGSQIVFCRTKIPWASQRDPLPWDVVVMDAENGKELKIIPDGNTPTWAADGKS
                     VYFQRRASLFVRHSLEDGTETILFDSGDPKVHLQTPDYNEDARRLAVTLRGRQRDTVL
                     LGRGEERLRVGSGRGCQLAWSPDHGFLYYVDHGGQMKNAVWKLESRRAGGALWLDLPL
                     PWSHEYFPRLSPGGKYMVLGACEKGHEHDAADYEIFLWPVDQPAEAAVRITHHTGNDC
                     WPDIFIR"
     gene            complement(8163..10697)
                     /locus_tag="PKK36_00550"
     CDS             complement(8163..10697)
                     /locus_tag="PKK36_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93079.1"
                     /translation="MTSRKYFPAAASLLITLAVWMIFTWPLPRFAASGIPAAHHRSDT
                     PAERIMFPGDHLQLLYHFWLAGDMLFGETPVFHNVYEFNTGDDSTRFEPGAYYAPFSV
                     VWALLNLSFSRALAWNLTDLFSLWLTAFFTWKLASRYTPRWPLAALAALLGIALPYRW
                     VSLLGGSPTGFAMAWIPMLWLGLDDAIRRERFRAGLLAGLVILFACWTDTHVFFFSVL
                     SIPVWGAIALIQRGRPRTRADWKRLAAVLLPVAAGVLLSYVFTKWVALSLDESVTAGG
                     RKISEVRLFSPVAAGLFSRRDMGLSSQVYLGFAAVLLAAGGFVAQVWQLRTARAGLRR
                     TGITGLLLLCIAACAVLSLGPRGPFDGGIFLFVRKVIPPYEMIRQPAKIFALLPTLLA
                     MAVVTGWSSVRRRGLKHLTAARALAAAGVIIVAEYAAFCDPAICILQGDQRAYAAVAE
                     DASARGILPRALVLTLWPGDSHYTSVYQDYASLYRIRLINGYRPMVPRAYVENVFRVF
                     ESMNQGLISDDQLASLENMGIDYVLLHEDLYPEKVSPFPVGVTLDRLLSHPRLQLLRQ
                     DGPVWAFRILDAARPPVSDIPSETFAFPAWHWEAENLHRENCEIASDSGFSWLAFRTP
                     GASVITRPLLVSAFDENRWIIRIRGQGALSVRTIARAEENAVALDVDSPDWSWIAVPF
                     TRPSPFEELQIEIILAAGAVDVDQFYLAGRTPVLPEPGQNLEMPASAFFHAGFTDAAS
                     GSVVLRNDYDPSGSIFYGFNLPLEPGRYRLTLDLSAEDAAPGPLGKLSVIPVRGKKPA
                     ASVTVAQPGSTSVEFLVPDNHPLRFDFDYSRAADVTLRKLTLARLE"
     gene            complement(10694..11653)
                     /locus_tag="PKK36_00555"
     CDS             complement(10694..11653)
                     /locus_tag="PKK36_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR93080.1"
                     /translation="MKGIRKFIPLVKIAFSVCLLTFLYARADWTSFREIFSQASPVYL
                     LPIFILCFLNTLASAFKWELLLKADGIDVGLWRLARSYLIGTFFNLFLPSSIGGDAYR
                     IYDVSKRSSRAAEGFASVFADRFTGFLAIAAWGLVFSLAGIRRLPEPRIILIPAGVFA
                     LMMIMVWMLLQQRLLLWGLRLFHLDRLAKLQEFVRRFLNSISTYRRNGVLLVKTLSIS
                     LAFQFMVIVIVWMMGLTLGLDIGMLPFAIFIPLITLIEALPISIYGLGVRDGAYVFFF
                     SQVGVSREAALSLALLYVAITFVYAAVFGGAAFLTRGKREVPA"
     gene            11801..12763
                     /locus_tag="PKK36_00560"
     CDS             11801..12763
                     /locus_tag="PKK36_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNR93081.1"
                     /translation="MPYIQIARPDHWFKNIFVLPGIVLVYFFERLPLDFQFAVNVVTG
                     LVCACLVASSNYVLNEILDSAKDKFHPEKQFRPIPSGQVKLPLAWLEWVLLAVAGLGL
                     AFHLNWRFGAMAALLWIAGCCYNIPPVRTKDMAYADVISESFNNPLRMAMGWYSTGFT
                     ELPPVSILLAYWMFGAFLMAMKRFAEYRRIADPARAGQYRKSFAYYNEERLIESLFFY
                     GALFGMFSGVFIARYHVELVLATPLVAFLIAYYMHLGFKPDSVVQKPEYLYHERKLVL
                     LSVLAFLACAFLLMTEIPGFQSLIEPTKVSPGAHHLILTSGGNP"
     gene            12760..13128
                     /locus_tag="PKK36_00565"
     CDS             12760..13128
                     /locus_tag="PKK36_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNR93082.1"
                     /translation="MKSETVAILGASDNPERYSHKAFILLRENGHKVIPVNPSGKIIE
                     GIPAAQSPGDITEPVDTLTMYVSPGISSKLTDAILALKPRRIIFNPGTENPELQQKCA
                     AQGMEVIEACTLVMLRTGQF"
     gene            13189..13274
                     /locus_tag="PKK36_00570"
     tRNA            13189..13274
                     /locus_tag="PKK36_00570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13222..13224,aa:Leu,seq:taa)
     gene            13367..14380
                     /locus_tag="PKK36_00575"
     CDS             13367..14380
                     /locus_tag="PKK36_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR93083.1"
                     /translation="MKQPIKTAARRIRLRCALTGIAVALAGLVVFANILAWRHARALM
                     HYSDFTGIKPRLESVESEDKLAILFDGIEIPRPESDDTPDELAPDCVKILIEEPDGIT
                     LGAWYCNRGTSSPLVILFHGYTEDKTALIPEARIYLKLGASVLLVDFRGSGESSEDYT
                     TIGFKEGDDVTAAVNYARQSLQHTKIILSGYSMGAAAILRAVAANGISPDGIVAEAVF
                     DTMLNTVRNRFHMLNAPAFPGAELLVFWGGVQVGFDGFEHNPVEYAAAVQCPILFMHG
                     TDDPRALLSEGQNVYDAVPSQKMFRVFPNVAHQSYVEADPAAWEQAVKDLLLAADAIT
                     QEP"
     gene            complement(14364..15035)
                     /gene="rsmI"
                     /locus_tag="PKK36_00580"
     CDS             complement(14364..15035)
                     /gene="rsmI"
                     /locus_tag="PKK36_00580"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR93084.1"
                     /translation="METGVYIVGTPIGNLEDITARALRTLREANVIMAEDTRHTGILL
                     ARYEIRTPMVSCHKFNEARRFERIQSEFAQGRSIALVTDAGMPGISDPGARTVRMCRE
                     AGIPVWVIPGPCAASAAVSLSGMGDAGYHFEGFLPHKSGGRRRRLEELSHEPVPVVFY
                     ESPYRLIKLMNEIEQFVGPRQIFVGRELTKKFEEGISGTPAEIRASFEKRTVKGELVV
                     IMAPA"
     gene            complement(15091..15543)
                     /locus_tag="PKK36_00585"
     CDS             complement(15091..15543)
                     /locus_tag="PKK36_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR93085.1"
                     /translation="MNVLKSEIVRLARKEAKSETAPARRIMAAQRGLIADLRRQVGAM
                     QKELNVLKKNVASSGRAVLEKAQPKGRFWITGKGVRALRKKLGLTQADFARLAGVSVP
                     TIVNWESSKGKVSIRRKEIPARLQAMRGMNRRAAAEILGKRKSKRAKA"
     gene            15990..17921
                     /gene="dnaK"
                     /locus_tag="PKK36_00590"
     CDS             15990..17921
                     /gene="dnaK"
                     /locus_tag="PKK36_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNR93086.1"
                     /translation="MSKVLGIDLGTTNSCMAIMEGGKPVVIPNAEGGRTTPSVVAFTK
                     TGERLVGQAAKRQAVTNPLNTVFSIKRFIGRRFNEVDREKNLVPFETVAAANGDAHVK
                     AHEKMYSPPEISSMILQKLRADAEAYLGEKITRAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTAASLAYGLDQKKEEHIAVYDFGGGTFDITVLELGDGVFEVKATNGDT
                     HLGGDDLDQRVIDWLVAEFKKDQGVDLSKDPMALQRLKEAAEKAKCELSSSMETDINL
                     PFITADASGPKHLNVKLTRAKLEQLVEDLIERTRKPVMQCLQDAKVSPDKIDEVILVG
                     GMTRMPAIQAKVQEMFSKTPHKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLTL
                     GIETLGGIATALIERNTTIPTRKSQIFSTAADNQNTVEIHVLQGERKMASDNKSIGKF
                     HLDGIPPAPRGIPQIEVTFDLDANGILHVSAKDKGTGREQKISITASSGLSEQEIKNM
                     VKDAESHAAEDQKKRESIETRNQADAVAYQTEKFIKEQGEKIPADKKAKAEAAVERLK
                     QAIKDENADEMKSAMDAVNSEMQSISQDLYANASQQQPPPGAGPQADSGPSKGEEAEG
                     EVIDADFEMMDDDKGKKKK"
     gene            17993..18280
                     /gene="groES"
                     /locus_tag="PKK36_00595"
     CDS             17993..18280
                     /gene="groES"
                     /locus_tag="PKK36_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNR93087.1"
                     /translation="MQIQPLGDRVLVEPLKENEVKKGGIIIPDTAKEKPQEGKVIAVG
                     TGKLDDNGKVIPFNVKKGDRVLMPKYGGTEIKMDDKVYQIMREEDILGILK"
     gene            18298..19944
                     /gene="groL"
                     /locus_tag="PKK36_00600"
     CDS             18298..19944
                     /gene="groL"
                     /locus_tag="PKK36_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098456.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR93088.1"
                     /translation="MMAKQLKFDAAARQAILSGVEQLSAAVKVTLGPRGRNVIIDKKF
                     GSPTVTKDGVSVAKEVELKDPFENMGAQMVREVASKTSDVAGDGTTTATVLAESIYRQ
                     GLKNVTAGANPMELKRGIDKAVTAAVEKLQSISKRVKDNEEIRQVATISANGDDTIGT
                     IIADAMDKVGKDGTITVEEAKGIETSLDVVEGMQFDKGYLSPYFVTNAETMDVILENP
                     YILLFEKKISNLQDLLNLLQSIAKSGRPLLIVAEDVEGEALATLVVNKLRGTLQACAV
                     KAPGFGDRRKAMMEDIAILTNGKFITEDLGIKLENVEIGDLGTAKRVTIDKDNTTIVE
                     GAGKTADIQGRIAQIRRQIEETTSDYDREKLQERLAKLAGGVAVINVGAATESEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRTLPALDDLKVEGDQAIGLEIVRQALEAPI
                     RQLVENAAVEGAIVVQDVKKGKGAHGYDVAKGEFCDMIKCGIVDPTKVTRSALQNAAS
                     IASLLLTTECMITELPEKEKSPMPGMGGDMGGMGGMGGMM"
     gene            20027..20269
                     /locus_tag="PKK36_00605"
     CDS             20027..20269
                     /locus_tag="PKK36_00605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNR93089.1"
                     /translation="MNTEYLEKAKERIPNVPLLINLISRRVRQLNAGERSLVKPDTLN
                     MEKVDIALKEVAEGKLQAEMVFEADQTTSSERVITL"
     gene            20287..20769
                     /gene="smpB"
                     /locus_tag="PKK36_00610"
     CDS             20287..20769
                     /gene="smpB"
                     /locus_tag="PKK36_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNR93090.1"
                     /translation="MARSTRKPAGGGPLASNRKAFHNYTVLEKFEAGMVLRGTEVKSA
                     RAGRISIEESYAQVEDGQITVYELTIMPYEFGTSHNHNPKRPRRLLLHKAEIRRLHAQ
                     TVIKGLALIPLKIYLKHGHIKMEIGLCKGKQLHDKRDALRERTAKLEAARAMAAHRHR
                     "
     gene            complement(20770..21639)
                     /gene="rsmA"
                     /locus_tag="PKK36_00615"
     CDS             complement(20770..21639)
                     /gene="rsmA"
                     /locus_tag="PKK36_00615"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNR93091.1"
                     /translation="MTDFLTPLTSPFAVKAVLQELQMVPGKSMGQNFLIDRNILNIFV
                     DLAELKKSDSVLEIGPGLGTVTRELLARAGRVTAIEKDLRLCDYLRQTLSACSNLELM
                     AEDALEAPLEGMLAGGCGKLVSNLPYSCGTRILVRVITAPARPELVVVTVQRDVAERM
                     TAGPSCKAYGLLSVCVQTFYEAVVERVVSHGCFYPRPRVDSAIVRLRRRPEPLTGGLQ
                     PDFILRLLHEFFSKRRKQLGTLLSQIMPARGMKKEDMESLLVELGLDHQMRPENLSPA
                     QWAELLQALAARG"
     gene            complement(21636..22598)
                     /gene="pdxA"
                     /locus_tag="PKK36_00620"
     CDS             complement(21636..22598)
                     /gene="pdxA"
                     /locus_tag="PKK36_00620"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNR93092.1"
                     /translation="MKLIAVTAGDINGIGPEVALKALSACKWPSNCRFVLIGNAGMLS
                     LQARKFGLPVKSQSFSIWDPDGARLAWKPGKITASASRHAMHWVRLAAEGCMGGYFDA
                     MVTAPICKEGLKKAGLKAPGHTEYLAEITRTKRFAMMLMGGGLRVILATRHIPLSRVA
                     QSISRKNILEAIRITAEALPWLGCRRAKIGVCGLNPHAGDGGAIGREEKTIIHPAVLA
                     ARRAGLRAEGPVAPDAIFYHALRGAYDAVVAMYHDQGLGPLKMLAMDEGINITLGLPI
                     LRTSPDHGTAFDLAGKGRAREGSMLKALELACDLAGKKNPWRKK"
     gene            complement(22595..23572)
                     /locus_tag="PKK36_00625"
     CDS             complement(22595..23572)
                     /locus_tag="PKK36_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNR93093.1"
                     /translation="MRMPRRLTVFFLFGICLCRPVAGAERAPVVADGFAALVNDRVVT
                     VSDVLMLVQQGETQRQTLYEGAELEQKRKQDFEAALNALIDQALILEDFEQQKAVIPD
                     RVVDEYLESTIREKFDNDRGAFLSELAAERLTLEDYREQIQDRLIIMISRQQILRGNI
                     FVSPSSVRRVYEERADRYGTPEAVHLRVITFSGGKTEEDRAGSRSLAESVRAQLADGA
                     DFAELAAEHSQDSRAKQGGDWGWVAVRDLAPDFAEALKSLQPGGVSAVVGSRDLYILK
                     LEERRQAAVRPFDEVKDQIEKELRSAEEERIYREWIARLRKKHTVKIFE"
     gene            23688..24329
                     /locus_tag="PKK36_00630"
     CDS             23688..24329
                     /locus_tag="PKK36_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNR93094.1"
                     /translation="MTNHISIVLASSSPRRQEMIRGLGLGFQIIKPDVDETPEPGETP
                     SDFALRAARDKARWVAEKIIRDGTIAQPVLVISGDTIVTLGNRILGKPRDEAHARHML
                     RELSGRSHDVISGLCVLKAENNAIVHSESMTVSTEVTFKTLSDEEIEAYMATGEVMDK
                     AGAYGIQGACSYMIESIRGSYTNVVGLPLCELAGILMHDYGLHLSLAPQKANA"
     gene            24344..25891
                     /locus_tag="PKK36_00635"
     CDS             24344..25891
                     /locus_tag="PKK36_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93095.1"
                     /translation="MKRIAEAALIILLCVSVRLTGIRHGAPDLIFHPDVAKQAFVARD
                     IYAGDYKPGYRYGENYQLALYPYGDSIMLAHELKGLTPVLHMAGLAREKLLNMHRWNW
                     AFMLRLHTAAWITLALAVFIMRAWPALSWKTRLPAALLLIFEPVNAQYSHYGMNDVPL
                     AALLMLAWLAAAAMDRDTGCRPWRSALCGLILGVAFGVKYQALLGILLPVTVWILLFR
                     EKNAVWRWAVAAAVATGFLAGALYACPPLRIPDYFMKHFPRFMAWQANITGEEISLMG
                     KISRNAALLVRFSFRYGYWLLLPPWVLLMLRAARKKLDRNTLLMAVPGFVFSTFLLFL
                     FIVSRDFIRSNDLVALAPFWIWPVALIKSPERRGPRIVFRAILTALVMAWISAAWLDS
                     LALRRTDTRIRARDWCVEHFHSRQTAIFERYTLRTRKPRVQEVQVRNLVSRQARRHMR
                     RGQYQWLVQSSIARDRFFDPLSPFFDREAQKNYLDIETGHVLMTEFRDRELFFAHPRI
                     RIYTPAP"
     gene            25891..27474
                     /locus_tag="PKK36_00640"
     CDS             25891..27474
                     /locus_tag="PKK36_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93096.1"
                     /translation="MSDQLTILSFPVGLAVVCLLAETAALLGLRRAAGGEKPVWPLLV
                     RWSARPWLVWAVFSMVYRLQPDKAFNNVRTFPFFSNLWHGHEGWMESFVRIATTPSAW
                     IFVPMTLLTLTALCMLARKISRPDSGVKTWKTLFAVYLLGLLLMLVIASLPDGPLLKE
                     NGRAGALTRSWQAHTTVLWALPHIRTKSHFLRNFEEIQPRLRGTIHGLSHPPGGALSL
                     YFIGKVIGADHEDYRSHMTRFQYLIGLALFSFLNVFVLYGLGYLLFDDRRTGLAAALL
                     WTAAPSTLAYSYFAQNGLYAVFFCLALLLTWTTVFKSDRPWRWAVPLGLVFYLLTMMN
                     YAWCIATSIFAVFALATGLQRRWRPADILIRAILPLGIMTLLLVSTLLYYRLDYLEIY
                     NVSSDYVEQWYRFTSPYRWLAALLGGQIDMWLMMGSATCSFFLAGAGAALRRKPLEPA
                     TVFLLIILGVFALPLLFGPNPLKMETARCWNWILAVPLAFAANRIMQLEKAEVLLPAV
                     TAVSGTTYLLMRLYLDFAP"
     gene            complement(27485..29191)
                     /locus_tag="PKK36_00645"
     CDS             complement(27485..29191)
                     /locus_tag="PKK36_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR93097.1"
                     /translation="MRKSTAWFVFGAVTALLSVLFFWPLGTVISGGFWVNGKFTLRYL
                     IGVFENPIYVEGLFNSLKIALGTTLLAMVVALPLAWIANQFEFRGKAAFSALVLVPMI
                     LPPFVGAIGFQQMFGAYGMINALFGLGARDWLGGGQYAGVVLLQTLSLYPILYLNAVA
                     ALANIDPAMEEAAANLGCTRLRRFFRITLPLMMPGLFAGGTLIFIWSFTELGTPLIMN
                     YTRCASVQIYDSLKEIGSNPFPYALVTVMLAASVGLYAVSRRLFGGRAYAMQSKASTQ
                     SAVRTLSGWRGLLAALPFALVTGLALLPHLGVVLTSFAAPGSWYQSVLPQTFTGGNYI
                     EALGHDMTVSSIRNSLLFSSLAVAFDIVVGIAIAFVVVRSKIRLRGVLDALSMIPLAV
                     PGLVMAFGFLAVSSWLSNLKGFADADWWPRLVDVRTNPTLFLVLAYSVRRLPYMVRSA
                     VAGLQQTSVTFEEAAWNLGASPAKTLRRITLPLIMANLIAGTLLTFAFSMLEVSDSLM
                     LAQQADYMPITKAIFELFQLLGTGKYVAAALGVWAMAFLTATLVGSSLLLGKKLGAVF
                     RV"
     gene            complement(29188..30960)
                     /locus_tag="PKK36_00650"
     CDS             complement(29188..30960)
                     /locus_tag="PKK36_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR93098.1"
                     /translation="MTRNLSILAVLAAVVALPFVFRQETGVREWREGDPVLVVITPMN
                     EAIRHEYALGFSRWHAENFGSPVKVDWRNIGGTTEISRYLTSEFVSSFRAWWTAQGRP
                     WRGDGAAIILSKSFNPDAKPGDVDEADWAEQCSMYAAFRGTDDPQAFSSRIDMYFGGG
                     AYDGDSATRQGLLVPAWAPEEIPPGLLATDAGAELIPPGMSGEVWRTPTWYGTTLSTF
                     GICYNRDRMLAQGMDRDPEHWADLADPRWFGTLGLADPTKSGSIAKAFETIVQVQCRH
                     AVEAAGFGPMIDDYELRIAAAKLAPGQMPEGVPEEYQQAVETGWMNGLRLIQKIGANA
                     RYFTDSASKVPLDVGMGNAAAGLAIDFYGRFEAQVSNAGHGRESMAYVTPRGESGVSS
                     DPISLLRGAPRREIAQRFIQYLLSEEGQKLWCYRVGEPGGPQKYALQRFPIRRDFYPS
                     DNPVFQAAHERHRAHTTEDMSRPSLDAYRLAEEYVYRPRWTAGHFGLFRDLVRAMCMD
                     AGKELRMAWGEIVARGGPEACPRAMDALQRLPLEPEPLTWTSGLALGRKYDRIDLLRE
                     WTIQYRAQYAEAARLAREEGGTRR"
     gene            complement(30957..32030)
                     /locus_tag="PKK36_00655"
     CDS             complement(30957..32030)
                     /locus_tag="PKK36_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93099.1"
                     /translation="MPVPITIEKLTKRFGAKTVLRDIDVQIAAGEIFFLLGPSGCGKT
                     TLLRAVAGLNDPDEGRILAGDRDVTHLPPHQRDMGMVFQSYALWPHMSLRENVAFGLE
                     MRGIGKADIQARVMRALEMVRLADRADSKPNELSGGQQQRVALARALVIEPQCLLLDE
                     PLSNLDAKLRLEMRTEIRRICKEAGLTAIYVTHDQKEALSIADRLAVLDQGRLLQIGS
                     PQEVYTRPGSRFVAQFIGETTFVEGRVLGWRDGRAEIETAAGIWTAQTAGGLEAGETV
                     WLSVRPEAVQLHTDRADGPNVFNAALHATIYLGEVAQHAVELGGDISLKALETRPRLV
                     ARDDARAETRLYVDPADVQVLKA"
     gene            complement(32227..33666)
                     /gene="rpoN"
                     /locus_tag="PKK36_00660"
     CDS             complement(32227..33666)
                     /gene="rpoN"
                     /locus_tag="PKK36_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNR93100.1"
                     /translation="MAQQILSQVQDQRLQMILAPQLRQSLEILQLPVLELQSVIQQEL
                     EQNPALEEYDAPQAAPETPAEPPAPEPETDNNKEMDFKEDYEILLKLDDDWREFYQQS
                     QTLQRWTPDDAERREHFLDSMVQSESLQEHLMAQLPMAGLEQEDRRIGELLIGSITSD
                     GYLSTSIEELAASTGYSVQKLNEVLAVIQGFDPVGVGARDLSDCLLLQLRRRGLDQSP
                     AANMVRLHLNDLAARKYARIAKEMNLPLETVQETARVIAALEPKPGRAFAAESTNFIH
                     PEITIRKVDGEYVVQLDDDHLPHIRISRHYHKLLEDPATPADVKNYVQEKIRSGMFLI
                     KSINQRQQTIYRIVRELVKTQRDFFEHGLSHLRPLTMSAIADELGIHETTVSRAISGK
                     YVATPQGTYDLRYFFASGYAHADGQQVSNKTVKEALANLVAKESPSHPLSDQALAGAL
                     RENGFNVARRTVAKYREELKILPSHLRKA"
     gene            33737..34522
                     /locus_tag="PKK36_00665"
     CDS             33737..34522
                     /locus_tag="PKK36_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR93101.1"
                     /translation="MRERVCVLASGSSGNCTFVSADGVSILVDAGISGRETERRLEEI
                     GEDPGRLRAICVSHEHTDHTAGIGVLHRRYATPLFANSGTINGIGRQRGLAGLPWNVF
                     TTGQPFQIGTLQIEPVSVPHDAYDPVGFIIRSSTAAIGVVTDMGMATALIRTRLRDCD
                     ALVIETNHDETMLQNAQRPWMLKQRIMGRQGHLSNRQAAAVIEEIAGPRLKQIFLAHI
                     SSDCNEAWRAEQEIRQTVEHAGLAHIQIACTFPDRISAVWQPA"
     gene            34600..35703
                     /gene="ugpC"
                     /locus_tag="PKK36_00670"
     CDS             34600..35703
                     /gene="ugpC"
                     /locus_tag="PKK36_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNR93102.1"
                     /translation="MAKVILEKVCKVYPGSVTAVSDVNLEIADREFVVLVGPSGCGKS
                     TTLRMVAGLEEVSSGSIYIDDRKVNDVPPKDRDIAMVFQNYALYPHMTVRENLAFGLK
                     LRKMPRADIEQRVGEAADILGIADLLERKPKALSGGQRQRVAVGRAIVRKPKAFLFDE
                     PLSNLDAKMRVQMRMEISRLHTRLACTMIYVTHDQIEAMTMGDRIVVMKDGVVQQTDR
                     PMTIYSQPANRFVAGFIGSPPMNFFDGRLENRDGRMFFNAGTFSVCAEDSQYARLAEH
                     DGKKITMGLRPEDLKDALYAPQASADHSVPAGVEVVEPMGAEVYLYLNNGPHNFTART
                     DAHDPATAGQSITMVFNMKKAHFFDAETGRTIL"
     gene            35724..36836
                     /locus_tag="PKK36_00675"
     CDS             35724..36836
                     /locus_tag="PKK36_00675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNR93103.1"
                     /translation="MMLDQIMTVIGQTLIVVAVSFIMLCLLVSYARFHLLFTGRRPSA
                     AAAEGVLDPFRACILKQLGSIYRNPEPFAVMTLRATDGGHRPEDPVARIRGMIRASDM
                     VMRYDENQIGMIIKTSRKHAERVAQRVADGLPGSPAIGIATCPENGTRLSDLLENAEH
                     AAEEAGRLSGGVKLAGEPVTAPDEGDILPAQEKYLDDLTGVLRGDLLGRTLPKFISQH
                     RKEGRTVAMIYCDVDNFQDYNQHYSREAGDALLQGVSRLFQENLREDDLIARYSGDEF
                     LILFPAAAADAQHTARRLSAVIKRAEFKYGAITLKTTVSMGIALVPGHARTLSRLFEA
                     ASHALHAAKQKGVGFCVMYAPGMGVQSRTQDVIEKF"
     gene            36848..37576
                     /locus_tag="PKK36_00680"
     CDS             36848..37576
                     /locus_tag="PKK36_00680"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNR93104.1"
                     /translation="MKLGVNIDHVATIRQARGTRYPDIVEAAEACRRGGAHGITIHLR
                     EDRRHIQDADVFAVKKWGRLPLNLEMANAEEIVAIALKVKPEEVCLVPEKRRELTTEG
                     GLDVVRDRKLKQTVARLSEAGVRVSLFVDPDPRQLEASLKVGATCVELHTGRYCDTRG
                     TIRKRELRRLAAGAKLAHKLGLQVNAGHGLNLDNLDGLASVPHLDTLNIGHSIVARAV
                     LIGLEPAVREMLRAMKGIQQHAGR"
     gene            37563..37988
                     /gene="acpS"
                     /locus_tag="PKK36_00685"
     CDS             37563..37988
                     /gene="acpS"
                     /locus_tag="PKK36_00685"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNR93105.1"
                     /translation="MPAGNTDPRGVAGIGIDIVENRRVEDALARHGRRFRNRIFRKSE
                     QDYCDQKARPALHYASRFAVKEAVAKAFGTGIGAELGWLDIEVVRDGGGAPVVMMLGK
                     GAELARKMNIDRIYVSLSHTHQYAAATALLAVKCPRGPA"
     gene            37985..39478
                     /locus_tag="PKK36_00690"
     CDS             37985..39478
                     /locus_tag="PKK36_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNR93106.1"
                     /translation="MIMVSSAQMRELDRRTIEEAGVPQVVLMDRAGHAVADHICWLLR
                     AAGISHPYIRFLAGPGNNGGDAIVAAQYLQKRGYRTDIWTFGRPSEASGLDIHIISGE
                     ADWEQISAGAFIPDLIVDGLLGTGTKGAPRGLIRKAVEWIQSLSGKTLIVAIDIPTGL
                     DADTGAANDPAVHADLTITLGAPKTGLLRPEALSCIGSLEVADLGIPEEYFRALNKPA
                     GPDLICANNLRALFPRRRADAHKGLFGHALLIGGSPGFSGAISMAARAAVKSGAGLVS
                     AAVPESIVSIVAPSCPEAMVHAMELSDSLLEKCTSVLIGPGLGNNPEAARLVERMLKT
                     SSVPLVLDADAINALSGRPAIIKKEKCPVVMTPHPGELAGLMQTTAAAIQADRCKACL
                     AAAEKSGAIVILKGAGTLIADPAGELFINLNGNPGMATGGSGDVLAGILTGLLAQGIR
                     PLDAAKAAVWIHGKSGDAAALRRTQTALSAADIINNIPSTFRRLTPR"
BASE COUNT         8474 a        11763 c        11759 g         7537 t
ORIGIN      
        1 ttcggtcaaa atacattttg acaaacccga tcatgcggcg tacgttttac cgtacgtata
       61 taaatggagt taattatgac aacccttaca gcaacagaag caagaaagca gctatacacg
      121 ttgctagatg acgtgtcgga ttcgcatgag cctgttcaaa tagcaggaaa aaggaactct
      181 gctgtcctaa tttccgacga tgattggcgt gctattcagg aaacccttta tctgacatca
      241 atttccggaa tgcgcgaatc tattgtcaag ggcttgaaga ctaaagttga agcgtgctcc
      301 aaggaactcc actggtgagc tggagcatcg tttatactaa tcaggctaaa agggatgcca
      361 aaaagatttc caggtccggc cttaaaccac aagccctcaa gttgctggaa atactggagc
      421 gcgaccctta tcagaatcct cctgcatatg aaaagctggt aggtgatttg cacggcgcct
      481 gttctcgcag gatcaacata caacaccggc ttgtttatca ggtgatggac gatattaaga
      541 ccgttaaagt aattcgtatg tggacacatt acgaatagcg aaccacaggc tggagtctac
      601 cggtgccccg ccgcgcgtcg ctgaagctat gcagggcaag cctgcggcgg aaacaccgga
      661 gactcacccc taacgttggc cacaaacaac aatctgactt gtttttgatg ccccaggggt
      721 tatattgagc actatgacaa cggtacaaga aattgaaaaa gcggttcaga gtctgcccga
      781 gcccgaattg ggaacttttc gtgcctggtt cgaggatttt gacgcgaacg cctgggataa
      841 gcagtttgat caggatgccc aaagcgggaa acttgatgct ttagcggatg aggcgctcag
      901 ggatttcaag tctaaccggt gtacccggct gtgaatcact ttgcctcccc gcgtttctgg
      961 gatcattatc actcactacc cgccgcgatt cgaaaactgg cagacgataa ttttgttctc
     1021 ctgaaaagca acccacagca tccgtctttg catttcaaga aggtcggtcg ttttcattca
     1081 gtacgcattg gacggaagta tagaaccgtg ggggtgctgc gcgatcaaga tattgtttgg
     1141 ttttggatcg gcactcacgc tgattatgat caaatcataa aatagggcga accacgggct
     1201 ggagtctgcc ggtgccccgc cgcgcgtcgc tgaagctatg cagggcaagc ctgcggcgga
     1261 aacaccggag actcacccct gacgttgtgc aaatatatga tggcctgttg acaatgtata
     1321 acatatgtaa tacggtgtga ttatgaaaac agccctgata catgcaagaa ttgaacccga
     1381 aacaaaaaaa cgcgcggaga ttgtgttaga tcggcttggg atgacaccca ccgaggccat
     1441 tcggcttttc taccatcaaa tatctcttcg caaggggttg cccttctctg tcgagattcc
     1501 gaatgagtta accgcacaca ccctgcacaa ttccagcaag ggtaaagaca tacagaactt
     1561 tgaaaatctt gacgatctgt tcgccagttg gaacaaatga agagtgtttc ccaaaccaac
     1621 cagtttgcga aggacatcaa aagaatgatt aggcggggca aagaccccgc taagatgcag
     1681 accgttgtcc gaaaactggc aaatggagaa gttcttgata gaaggcatcg ggatcactct
     1741 ttagtcggag agtgggattc gtttcgtgat tgccatattg aaccggattg ggttctcatc
     1801 tattttacag atgcagaaaa gctcattctt caacgaaccg gaacacattc cgatctgttc
     1861 aagacatgac atgcacaacc acgggctgga gtctgccggt gccccgccgc gcgtcgctga
     1921 agctatgcag ggcaagcctg cggcggggac accggagact cacccctgac gttcgggaat
     1981 acatataatt agccatatat taatattgct atttgcgagc tgcgtgatat tttacggtta
     2041 tgggaagaac gtcatttgaa tgggatccgg ccaaagaccg gcagaaccag aaaaagcacg
     2101 gggtcccatt tgttcttgcg caatatgcgt ttgctgatcc gaaacgcgta attctggttg
     2161 acggagaaca cagcaccgag gaacagagat tctattgcct tggggtcgtt ggagaaggaa
     2221 tcctgacggt acggttcacg tatcggaaca atgtgatccg acttttcgga gccggatact
     2281 ggagaaaggg gaaaaagatt tatgaagaac agaataaaat acacggatga gcccatggat
     2341 aaggtacggc ccgtgaagga ctttcttcct tcacccgatc agcttgcctt caaagaagag
     2401 aaggtcaagg tgaccatttc tctcagcagg gcgagtcttg aatttttcaa gatgcaagcc
     2461 aatcggcatc atacggcata tcagaagatg attagaaatc ttctcgatgc atatgccgga
     2521 cagcacatga aataacccga accacgggct ggtgtctgcc ggggccccgc cgcgcgtcgc
     2581 tgaagctatg cagggcgagc ctgcggctca ggcaccggag actcacccct aacgttgtgc
     2641 aacattcatc aattgacact gtatcccact tgtggtacgt tatattggaa catggagaca
     2701 agatcatgag caaaagcgca atggtaagag cccgtctaga gccgggtcta aaaaatcacg
     2761 cggagcacat ttttcatcgc cttgggctta atgcaactca agcaataacg atgttctaca
     2821 agcaggtgga gctacaaaag ggccttccct ttaatgtggc catcccaacg gccgcaacgc
     2881 acaacacctt caaggcgaca gatgagggac gggatcttgt catctgcaac gatgcggacg
     2941 acatgtttga taagctgggt atatgatgta tatcccggtt tataccaagc agtttgaaaa
     3001 ggacattaaa cgctgtaagc ggcgaggcaa gaatctaaac aaattcaaga tgatcgcaac
     3061 atctctgctt gcggggaacc ctctagaccc catacatcgt gatcataaac ttgttggcaa
     3121 ctactccggg cggcgcgatt gtcatatcga gtcggattgg ctgctgattt acaagattga
     3181 ttgcgatcat atcatttttg aacgcacggg atcacattcc gaccttttca agaaatagca
     3241 tgcacaacca cgggctggag agtctaccgg tgccccgccg cgcgtcgctg aagctgtgca
     3301 gggcaagcct gcggcgggga caccggagac tcacccctga cgttaataca tatatgagca
     3361 gcgcaagata gaaatggatt ggagagcata cattccgcac ggcctgtttt ggggcgcaat
     3421 gattggcgtt attgtcggac tctcaataca accaattcga aaacatcttg cccggaagag
     3481 aatgcatatt ttggcaaaca gcgtccgagg cagtctcccc caaagtgatt cttttgtcat
     3541 cccgggaaag caccccggac accatcgcaa gtggcggatt gtaaagccaa aaggacatgg
     3601 atactacagt tcgattgaat aaacagccca accacgggcc ggagtcaacg ggctgaacag
     3661 atcatgacgc gctccagcga agataaaata caaaacgcgc ttcggccgat cgcctcgctg
     3721 atcagcaagt ccgagaaggc acagaggaaa ctggcggaga acacgtggca gcacgccatg
     3781 ctgcgggaca atctgaaagc gctgcgcgct gcactggcgc taatgaccag gcccgccgac
     3841 gatgcacacg gttttacgcc ccatgattta caggacgccc tccgctcgct gtcttcgatg
     3901 attcgcaaaa cgctgaaggc ccgggcggca tttccgcgcg ggacttccca gcacacgctt
     3961 cagcgaaacc ggcttgcggc gctgcaggcg gcggaagctg tgatcaaagc ggcacaaccc
     4021 gggcatcggg ccgcaaagcg aacccgaaat cacgaaacgc gcgctccagc cgcttcggaa
     4081 tccggcaggc atccccgcgg gacatccacg agggacgatt tcagtacgcg gaaccgccag
     4141 aagccgacaa tgcggcggcg aggccgccgg cagccatgat cagcatgacg gtgagcacga
     4201 tgccgatgac catccagatg agcgaggctt tggcccagtt ggacttgctc tcgggcgcgc
     4261 cgccgccaaa ggcccaaacc agaagcatga tgatattcac cagcggaatg cacatcagca
     4321 gataggtcag catccagtca ccaactgata cgggtctgtt ttccatttta tcctccctgg
     4381 gttaatatga cttcgcaaaa atcacgcggc ggcggctggg cttgccgctg atgacgcacg
     4441 tcccctcttc ctccggcgca tcctgcggga tgcagcggac ggtcacgttg agttcctcgc
     4501 gaagctgatt ctcaacagct tcctccccgc tccagtgcga aagcgcaaat ccgccgtgaa
     4561 tttcgggttt ttcggcgttt ttcggcgtaa aataggcata aaaatcgtct tttttatcga
     4621 ttttgaccgt attttcgtcc cgaaaagcct ttgcgcgctc aaaaagtcca ttctggatct
     4681 cgtccagaag cgtggcgaac gttgcgacca gttccgcgcg gggaacaccc tttttgtcgc
     4741 gtgcctcccg gtcgcggcgg cccatgaata cggaatcgga agccatgtcg cgcggtccta
     4801 tttccagccg gacgggaatt ccttttttga tccacgacca gactttttcg ccgccgctga
     4861 tgtcgcgggc gtccacctcg acgcgcacgg cgcggccgtc gtactgcacg gcgcgcagtt
     4921 cggaagccac ccggtcgcag tattccagga ccgcggcgcg gtcggcatcc ttgtgaatca
     4981 cgggaagaat cacaacatgg gcgggcgcga ggcgcggagg cagcaccaga ccgttgtcgt
     5041 cgctgtgcgt catgatcagt ccgcccacga gtcgggttga aacgccccac gacgtggtcc
     5101 atgccagcac ctcggatccg ttttcgtcct ggaacttgat atcggacgcc ttggcgaatt
     5161 tctgtccgag gaagtgcgat gtgccggcct gaagggcctt gcggtcctgc atcatggctt
     5221 cgatgctgta ggtgttcacc gcgccgggga agcgctcgcc ggcggttttt tcgccgggga
     5281 tgacgggcat ggccatatag ttctgggcaa agcgggtata aacgcccagc atcttccgcg
     5341 tttcctcctc ggcttcggcc tgcgtggcgt gggccgtatg cccctcctgc cagaggaatt
     5401 ccgttgtgcg caagaacagc cgcgtgcgca tttcccatcg cacaacattg gcccactggt
     5461 tgatgagcag cggcagatcg cgataggact gcacccattt tgaaaacatg gcgccgatga
     5521 tggtttcgga ggtcgggcgc accaccagcg gttcatccag cgggctggag ggtttgagca
     5581 ccccgcttgc atccgcctcg aggcggtggt gcgtgaccac ggcgcattcc ttcgcgaagc
     5641 cctcgacatg ctcggcctcc ttctgcagga agctcatggg gatgaagagc gggaaatagg
     5701 cgttgcggtg gccggtttcc ttgaacatgc ggtcgagcac gaactggatg ttctcccaga
     5761 tcgcatagcc ccagggcttg atcaccatgc atccgcgcac cggcgaagtc tccgccagat
     5821 cggcggcctt gaccacctgc tgataccact ccggataatc ctcgtcccgc cgcggcgtaa
     5881 ttgccgtttt ttcctgcttt gccataaact gctcctgaac ccgttttttc caatgattgg
     5941 aagtctgttc aaaaaaggtt ccaaccattg gaagttttcg cgcaaaaagt tccaatcatt
     6001 ggaagttttt ttcctgaaag ttccaatggt tggaaaaatg cccgtttcgc gccggtttta
     6061 caaccaataa agtggcctgc ggagagtcag cggatgaaaa tatcgggcca gcagtcgttg
     6121 ccggtgtgat gcgtgatgcg gacggcggcc tccgcgggct ggtccacggg ccagaggaag
     6181 atttcataat cggcggcatc gtgctcgtgt cctttttcgc acgcgcccag caccatgtat
     6241 ttcccgccgg gcgaaagacg cgggaaatat tcatggctcc acggcagcgg aaggtccagc
     6301 cagagcgcgc cgccggcgcg gcgcgattcg agcttccaga cagcgttttt catctggccg
     6361 ccgtgatcca cataatagag gaaaccgtga tccggagacc atgcaagctg gcagcctctg
     6421 ccgcttccaa cccgcaggcg ctcttcgccg cggcccagca ggaccgtgtc gcgctggcga
     6481 ccccggagcg tgacggcgag acggcgcgcg tcctcgttgt agtccggggt ctgcaggtgg
     6541 accttcggat cgccgctgtc aaacaggatt gtttcggttc cgtcttcgag gctgtgccgg
     6601 acaaacagcg aggcccggcg ctggaaatac accgatttcc cgtcggccgc ccaggtgggc
     6661 gtgttgccgt cgggaatgat tttcagctcc ttcccgtttt ccgcatccat gaccaccaca
     6721 tcccacggga gcggatcccg ctgcgaggcc cacggaatct ttgtccggca gaaaacgatt
     6781 tgtgacccgt cgggcgaaat gcgcggatag gtttcggtgt gaggatggct tgtgagcggc
     6841 cggatttttc cggacggcag gtccattttc catatgtcgt gattgccgcc gcggttggag
     6901 ctccagacga tgaacccgtc aagttcccgg agagcctgcc gcagtttgtt ccgctgctcg
     6961 cgcgaaagac cctcctcatg ccccgccttc gggggtgtca ggctcccgct ttttttgatg
     7021 ccgcgccagg agaaagcggc cgcagcggcg gcaaagacca gcaggatcac ggctgcggac
     7081 gcgatgagaa tttttcggcg ggttattgtt ttcatttctt tctccgtctt tggcgagccc
     7141 gcgctgaagg tgaacaccac gagggccatc ccgccgaggg tgtagtcaat gaactggctc
     7201 agcacatgca tgctgagcat cattacaatg gacacgtccg ccggaaagcc gaggagggtc
     7261 agcgcggcga ggccgcccgc ctcgtaggtt ccgaagctca tgaacgtcgg cagcagggca
     7321 aggctggctg tgccttcggc cgtaatcagc gcgatgatca cggccacggg cgaagcctcg
     7381 gcgaagtgtc cgccgaacgc gggcgcaatg ccggcaaaaa gaagatagag tcccgcgtat
     7441 ttcacgaggc gcacgccgag ggaaagcgcg accaccgcgg tcaggcggcc cgacctgcgg
     7501 gtgaaggtga aggcgtccgc cagccggtgc aggaaaccga acattctgtt cagcgcgggg
     7561 cgctggaacc gcacggcgcg gagctttgcg gagatccagc ccggagcgta cagcagggcc
     7621 atccatccgg ccagggtgat cacaagcaga atgcccgtgc tgatcagcag cagctgatgg
     7681 gcggactgac cgagaacggg cagcgcgatg atcacagcgg cgatcagcag cagcgccacg
     7741 aagtcaaaca gcatgctgag cgcgagggat gaaacacagt cctcgcccct gacgcgatag
     7801 cccttgttga gcatggcgat atagctcagc tcgccgaggc gcgccgggag catgtccacc
     7861 agcatgttgc gcgtcatggt ggtcaggtag agatgaaaaa gcgacggcac gttctgctcg
     7921 ccgcccgcct tcagcagcac cccgtagcgc acggagcgca gaaaggcctg caggagctgg
     7981 cacagcagat aaatgccgac ggcggcgagg gaggcgttcc gaacggctcc gaccacctgc
     8041 gccgcgctga ctccgggatg actggcggaa accagacgga aaatcagcgc gatcacaccg
     8101 aaggagatgg cggcggaata aagtattcgt atgaaaatgt cacggcgacg gatcatgatg
     8161 aattactcca gccgggcaag ggtcagttta cgcagggtga cgtccgccgc gcgcgaatag
     8221 tcgaaatcga agcgaagcgg atgattgtca ggcacgagga actccacgga agtgcttccc
     8281 ggctgcgcca ccgtgacgga ggctgcgggc ttcttcccgc gaacagggat tacggaaagt
     8341 ttcccgagcg ggccgggcgc cgcatcttcc gcggacaggt cgagcgtcag acggtaccgc
     8401 cccggctcca ggggaagatt gaagccgtag aatatggaac cggaagggtc atagtcattt
     8461 ctgaggacaa cgctgccgga tgccgcatcg gtgaatcccg catggaaaaa ggcggaggcc
     8521 ggcatttcaa ggttctgacc gggctcgggc agaaccgggg ttctgcccgc cagataaaac
     8581 tggtccacat ccacagcgcc cgccgcaagg ataatttcga tctggagttc ttcaaacggc
     8641 gacggccgcg tgaacgggac cgcgatccat gaccagtccg gactgtccac gtccagcgca
     8701 acggcatttt cttcggcgcg cgcgatggtt cgcacgctca gcgccccctg cccgcggatg
     8761 cggatgatcc agcggttttc gtcaaaggcg gagaccagca acgggcgcgt gatgacagat
     8821 gcgcccgggg tcctgaatgc gagccaggag aagccgctgt cagacgcgat ctcgcagttt
     8881 tcgcgatgca gattctccgc ctcccagtgc caggcgggaa acgcgaaggt ctccgaagga
     8941 atatccgaca caggcggccg ggcagcgtcc agaatccgga aggcccagac cggtccgtcc
     9001 tgccgcagga gctgaagccg cggatggctc agcaggcggt cgagcgtgac gcccaccgga
     9061 aagggactga ctttttcggg ataaaggtct tcgtgaagaa gcacgtaatc aatgcccatg
     9121 ttttcaaggg aggcgagctg gtcatcgctg atgaggccct ggttcatgct ttcaaacaca
     9181 cggaagacgt tttccacata ggcgcgggga accatcgggc ggtatccgtt gatgaggcgg
     9241 atgcggtaaa gcgaggcgta atcctgatag accgaagtgt aatgagaatc gcccggccag
     9301 agggtcagca caagagcgcg cggcaggatg ccgcgcgcgg atgcatcctc cgcaacggcg
     9361 gcgtaggccc gctgatcgcc ctgaaggatg cagatcgcgg gatcgcagaa tgcggcgtat
     9421 tcggcaacga tgatcacgcc tgcggcggcg agcgcgcgag cggcggtcag gtgcttcagc
     9481 ccccgcctgc gcacagaaga ccatccggtc acaacggcca tcgcgagcag ggtcgggagg
     9541 agcgcaaaga tttttgcggg ctgacggatc atctcatagg ggggaatgac cttgcggacg
     9601 aacaggaaga ttccgccatc aaacgggccg cgcgggccca gcgaaagaac tgcgcaggcg
     9661 gcgatgcata gcagaagaag ccccgtgatc cccgtgcggc gcaagccggc ccgggctgta
     9721 cgcagctgcc agacctgcgc gacaaagccg ccggccgcga gcaggaccgc ggcaaagccc
     9781 agatagacct ggctgctgag acccatgtcc cggcggctga acaacccggc ggccacaggg
     9841 gaaaaaaggc gcacttcgga aattttgcgg ccgccggccg ttacagactc atccagcgac
     9901 agggccaccc attttgtgaa gacgtaggag aggagtacgc cggcggccac gggcagaagc
     9961 acggccgcaa ggcgtttcca gtccgcgcgc gtccgcgggc ggccccgctg aatcagcgca
    10021 atggcgcccc acaccgggat gctgagcacg gagaagaaga acacatgcgt atccgtccag
    10081 caggcaaaga gaatgaccag gccggccagc aggcccgccc gaaaccgttc gcgacggatc
    10141 gcatcgtcca gtccgagcca cagcatgggg atccatgcca tggcgaagcc ggtggggctt
    10201 ccgcccagaa gactgaccca gcggtacggc agggcgatgc ccagaagcgc ggccagcgcg
    10261 gcgagcggcc agcgcggcgt atagcgcgag gccagcttcc atgtgaaaaa cgcggtgagc
    10321 cagagagaaa agaggtccgt cagattccat gccagcgccc gcgagaagga caaattcagc
    10381 agagcccaca ccacggagaa cggcgcatag taagcgccag gctcaaaccg ggtggaatcg
    10441 tcgcccgtgt tgaactcata gacattgtgg aagacaggcg tttctccgaa gagcatgtcg
    10501 cccgccagcc agaagtgata gagaagctgg agatgatcgc cggggaacat gatgcgttcg
    10561 gcgggcgtgt ctgaccggtg atgggcggcc gggatgcccg aagccgcgaa cctgggcaac
    10621 ggccatgtga aaatcatcca gacggccagc gtgatcagca gtgacgccgc ggcggggaaa
    10681 tattttctgc tggtcacgcc gggacctccc gtttcccgcg cgtcaggaag gcggcgccgc
    10741 cgaagacggc cgcataaacg aacgtgatgg ccacatacag cagcgcgagg gacaaggccg
    10801 cctctctgct gactccgacc tggctgaaga aaaaaacata ggcgccgtcg cgcacgccca
    10861 gcccgtagat ggaaatcggg agcgcctcga tcagcgtgat gagcgggatg aaaatcgcga
    10921 acgggagcat gccgatgtcc aggccaaggg tcagccccat catccacaca atgacaatca
    10981 ccataaactg gaaagccagc gaaatggaaa gcgtcttgac cagcagaact ccgttgcggc
    11041 gataggtcga aatggagttg agaaacctgc gcacgaactc ctgcagtttc gcgaggcggt
    11101 ccagatgaaa caggcgcagg ccccagagga gcaggcgctg ctgcaggagc atccagacca
    11161 tgatcatcat gagcgcaaac acgccggccg ggatcaggat aatgcggggt tccggaaggc
    11221 gccggattcc cgcaagggag aagacaagac cccatgccgc aatggccaga aaaccggtga
    11281 agcggtcggc gaacaccgat gcaaaacctt cggccgcgcg ggagcttcgc tttgagacat
    11341 catagatcct gtaggcatcc cccccgatgc tggatggaag aaacaggttg aaaaaagtgc
    11401 cgatcagata actccgggca aggcgccaga ggccgacatc aatgccgtcc gccttcagca
    11461 gcagctccca tttgaaggcg cttgcaagcg tattcagaaa gcacaggata aaaattggaa
    11521 ggagatatac ggggctggcc tgcgaaaata tttcacggaa agaggtccag tccgcgcggg
    11581 catacagaaa agtcagcagg cacacgctga aagcgatctt gaccagcgga atgaatttcc
    11641 tgattccctt cataaaagtc cgttaagaga gcaaggctag tgaggggcgc gcccaccgtc
    11701 aaccctttca tcgggctttg agactgaaaa gtgcatagcg ttcaatcttt agtgtggaaa
    11761 aacccgcgat acgccgctac tctccgtcac catgaaatta atgccataca ttcaaattgc
    11821 gcggccggat cactggttca agaacatatt tgttcttccg ggcatcgtgc tggtgtattt
    11881 tttcgaacgg cttccgctgg atttccaatt tgcagttaac gtggtgaccg ggcttgtgtg
    11941 cgcctgcctg gtggcctcca gcaattatgt gctcaatgaa atcctggatt ccgccaagga
    12001 caagtttcat ccggaaaagc agttccggcc gattccgtca ggacaggtta aacttccgct
    12061 ggcgtggctg gaatgggttc tgctggcagt cgcgggactt ggactggcgt ttcacctgaa
    12121 ctggcgtttc ggcgccatgg ccgccctgct gtggatcgcg ggctgctgct acaacattcc
    12181 gccggtgcgc accaaggaca tggcctatgc ggatgtgatc agcgaatcct tcaacaatcc
    12241 gctgcggatg gccatgggct ggtacagcac cggcttcacg gaactgccgc ctgtttccat
    12301 tcttctggcc tactggatgt ttggcgcgtt cctgatggcc atgaagcgtt tcgccgagta
    12361 tcgcaggatt gccgatcccg cgcgggccgg acagtacagg aaatcgtttg cctactacaa
    12421 cgaggagcgc ctgatcgaga gcctcttttt ttatggcgcg ctgttcggca tgttttcggg
    12481 ggtgttcatt gcgcgctatc atgtggaact ggtgctggcc acgccgctgg tcgcctttct
    12541 gattgcctat tacatgcacc tcggcttcaa acccgacagc gttgtgcaga aaccggaata
    12601 tctttatcac gagcggaaac ttgtcctgct ttccgtcctg gcgttcctgg cctgcgcgtt
    12661 cctgctgatg actgaaattc ccggcttcca gtccctgatc gaacccacaa aagtcagtcc
    12721 cggggcgcat cacctcatcc tgacttcggg aggaaatcca tgaaaagcga aaccgtcgcc
    12781 attctgggcg caagcgacaa tccggagcgc tactctcaca aggcgttcat cctgctgcgc
    12841 gagaacgggc acaaggtgat tccggtcaac ccctcgggaa aaatcattga aggcattccg
    12901 gcagcccaat cgcccgggga catcacggag ccggtggaca cactgacgat gtatgtctcg
    12961 ccggggattt cgagcaaact gaccgatgcg attcttgcgc tgaagccccg gcggatcatc
    13021 ttcaatcccg gcacggaaaa tccggaactg cagcagaaat gcgcggcgca gggcatggaa
    13081 gtgatcgagg cctgcacgct ggtcatgctg cgcacgggcc agttctgaag cgttttgcct
    13141 tgaagcacgg cagccgcggt tttagtctgc tttcccaatg acacaagggc ccgggtggcg
    13201 gaatggcaga cgcaagggac ttaaaatccc tcgaacgcaa gttcatgcgg gttcgactcc
    13261 cgccccgggc accaggcaga ggccgtctcg cgccgcaata tcttcctcat gcccggcgtt
    13321 tcgtatggac ttttccggga atttgtcctg aatatccggc gtgaacatga aacagccgat
    13381 caaaacagcc gcgagaagaa tccgcctgcg ctgcgcgctg acggggattg ccgtcgcgct
    13441 ggccgggctg gttgttttcg caaacatcct tgcgtggcgg catgcgcggg cgctgatgca
    13501 ctacagcgat ttcacgggca ttaaacccag gttggaatcc gtcgaatccg aagacaagct
    13561 ggcaatcctt tttgacggca tcgaaatccc gcggccggag agtgacgaca cgccggacga
    13621 gctcgcgccg gactgcgtca agattttgat cgaggagccg gacggcatca ctctgggcgc
    13681 atggtactgc aaccgcggga ccagcagtcc gctggtgatt ctgtttcacg gctacacgga
    13741 ggacaaaacg gctctgatcc ccgaggccag aatatacctg aagctggggg cctctgttct
    13801 gctggtggac ttccgcggat cgggcgagtc ctcggaggac tacaccacca taggctttaa
    13861 ggaaggcgac gatgtcaccg cggccgtgaa ttatgcccgg cagtcgcttc agcacaccaa
    13921 aatcatcctt tccggctact ccatgggcgc ggcggccata ctgcgggcgg tggcggccaa
    13981 cggcatcagt ccggacggaa ttgtcgcaga ggccgttttt gacaccatgc tcaacacagt
    14041 ccgcaaccgt tttcacatgc tgaatgcgcc ggcctttccg ggggccgaac tgcttgtttt
    14101 ctggggcggc gtgcaggtgg gttttgacgg gtttgagcac aaccctgtgg agtatgccgc
    14161 cgccgtacaa tgtccgattc tcttcatgca cggcacggat gatccgcggg cgctgctgtc
    14221 agagggccag aatgtctatg atgccgtgcc gtcacagaag atgttcaggg tgtttcccaa
    14281 tgtggcgcac cagtcctatg tggaagcgga cccggcggca tgggaacagg cggtgaagga
    14341 tctgcttctg gcggccgacg ccatcacgca ggagccataa tcacaacgag ctcgcccttg
    14401 acggtgcgct tttcgaacga ggcgcggatt tccgcgggcg ttccggaaat gccttcctcg
    14461 aattttttgg tcagctcgcg gccgacgaag atctgccgcg ggccgacgaa ctgctcgatc
    14521 tcgttcatca gtttaatcag ccggtaaggc gactcataga acaccaccgg caccggctcg
    14581 tgcgaaagct cttccaggcg gcgccggcgg ccgccgcttt tgtgcgggag gaatccttca
    14641 aaatggtagc ccgcatcccc catcccgctg agcgacaccg cggcgctcgc ggcgcaggga
    14701 ccgggaatca cccagacggg gatgcccgcc tcgcggcaca tgcggacggt gcgcgcgccg
    14761 ggatcggaaa tgccgggcat gccggcatcg gtcaccaggg caatcgaacg gccctgcgcg
    14821 aactcagatt ggattctttc aaagcgccgg gcctcgttga acttgtggca gctcaccatc
    14881 ggggtgcgga tttcatagcg ggcgagcaag atgcccgtgt ggcgggtgtc ctcggccatg
    14941 atcacatttg cctcgcgcag cgtgcgcagc gcgcgggcgg tgatgtcctc aagatttccg
    15001 atcggagtgc ctacaatata tacgccggtt tccatgattc aggattgtat cgaatggcgg
    15061 gcacaaagga agcagtcaaa gcggccggtt tcaggccttt gctcttttgc tcttccgctt
    15121 tccgagaatt tccgccgccg cgcgcctgtt catcccgcgc atcgcctgca gccgggccgg
    15181 aatttctttg cgccggatgg aaaccttgcc tttggatgat tcccagttaa cgatggtcgg
    15241 aacgctgacc cccgcaagac gggcaaaatc ggcctgcgtc aggcccagtt ttttccgcag
    15301 agccctgacg ccctttccgg taatccagaa ccggcctttg ggctgcgctt tttccagcac
    15361 cgccctgccg gaagaagcca cgtttttttt cagaacattc agttcttttt gcatcgcacc
    15421 gacctgacgg cgcaggtctg cgatcagtcc gcgctgagca gccatgattc ttctggccgg
    15481 ggccgtctcg cttttcgcct ccttgcgggc cagccggacg atttcagatt tcagtacgtt
    15541 cattacattt gccatttcag atactccttg tacacaatct tcagcacgcg gccattttac
    15601 agggaagcgc atccaacgca acacccaact gcgcttccgg acgagaaaaa tggcaaaaat
    15661 tggacatttt tgagcgtttt tcgggcattt ttggccattt ttgaccatat tttgttgttt
    15721 ttttgaaatt ttcgcatttt tttggagttt ttactgaatt ttgtcccggt gtgacaaaat
    15781 gtgtgccagc gcgccaaaaa gcgtcataat gtcacactcc gcccgctttc ggtcgcacgg
    15841 gcaaatattt atgatattta ttatatattg tcatttaatg gcttataaaa ggtctcggcg
    15901 gcttctatcc gaagttggca cggcacctgc tgaatcttga gacaggataa ttgcaattcc
    15961 ggggatgccc ggcagaaaag aggcacaaga tgtcaaaagt attaggaata gacttgggaa
    16021 caacgaactc ctgcatggcg atcatggagg gcggaaagcc cgtggtgatt ccgaacgccg
    16081 agggcgggcg caccactcct tccgtggtgg ctttcaccaa gaccggcgaa cggctggtcg
    16141 gacaggccgc gaagcggcag gcggtgacga atccgctgaa caccgttttc tccatcaagc
    16201 gcttcatcgg ccgccgcttc aatgaggttg accgcgaaaa gaatcttgtc cccttcgaaa
    16261 ccgtggccgc cgccaacggc gatgcccatg tgaaggcgca cgaaaagatg tattctccgc
    16321 cggagatttc ttcgatgatt ctccagaaac tgcgcgcaga cgccgaggcg tacctgggcg
    16381 aaaagatcac cagggcggtg atcaccgtgc ccgcctattt caacgacagc cagcggcagg
    16441 ccaccaagga tgccggccgc attgcgggtc tcgaagtgct gcgcatcatc aacgagccca
    16501 cggcggcctc gctggcctac gggctggatc agaagaagga agagcacatt gcggtgtatg
    16561 atttcggcgg aggcacattt gacattaccg tgctggaact gggcgacggc gtgttcgagg
    16621 tgaaggccac caacggcgac acccatctgg gcggcgacga ccttgatcag cgcgtgattg
    16681 actggctggt cgctgaattc aagaaggacc agggcgtgga tctctcgaag gaccccatgg
    16741 ccctgcagcg cctcaaggaa gccgccgaaa aggcgaagtg cgagctttcc agctcaatgg
    16801 aaaccgatat caaccttccg ttcatcacgg cggacgccag cgggcccaag catctgaatg
    16861 tcaagctcac ccgcgcaaaa ctggaacagc tggtggaaga cctgatcgag cgcacccgca
    16921 aaccggtgat gcagtgcctg caggacgcga aggtttcgcc cgacaagatt gacgaggtga
    16981 tccttgtcgg cggcatgacc cgcatgcccg ccatccaggc caaggtgcag gagatgttca
    17041 gcaagacacc gcacaagggc gtgaacccgg acgaagtggt ggcggtcggc gcggcgattc
    17101 agggcggagt gctcaccggc gaggtgaagg atgtgctgct gctggatgtg acgccgctga
    17161 cgctgggcat tgaaacgctg ggcggcatcg ccaccgcgct gatcgagcgc aacacgacca
    17221 ttccgacgcg caagagccag atattcagca cggcggcgga caatcagaac acggtggaaa
    17281 tccatgtgct tcagggcgag cgcaaaatgg cgtccgacaa caagagcatc ggcaagtttc
    17341 atctggacgg cattcccccc gccccgcgcg gcattccgca gatcgaggtg accttcgacc
    17401 tggacgccaa cggcattctg cacgtcagcg cgaaagacaa gggcaccggc cgcgagcaga
    17461 agatatccat cacggcctcc agcggactgt ccgagcagga aatcaagaat atggtgaagg
    17521 atgcggagtc ccacgccgcg gaagaccaga agaagcggga gtccatcgag acccggaacc
    17581 aggcggacgc cgtggcctat cagaccgaga agttcatcaa ggagcagggc gaaaagattc
    17641 ctgcggacaa aaaggcgaag gccgaggccg cggtcgagcg cctgaagcag gcgatcaagg
    17701 atgaaaacgc cgacgagatg aagtcggcca tggacgcggt gaacagcgag atgcagtcca
    17761 tttcgcagga tctgtatgcc aatgcctcgc agcagcagcc ccctcccggc gccgggccgc
    17821 aggcggacag cggtccgtca aagggcgaag aggcggaagg cgaggtcata gacgccgact
    17881 ttgaaatgat ggatgacgac aagggcaaaa agaagaagtg atttgagaac agcgggcggc
    17941 agatgccgtc ccggatcatt ggtaaacaac aaaaacagaa ggagaacgaa gcatgcaaat
    18001 tcaacccctc ggagaccggg tactggtcga gcctctgaag gagaacgagg tcaagaaggg
    18061 cgggatcatc attcccgaca ccgccaagga aaagccccag gagggcaaag tgatcgccgt
    18121 cggaacgggc aagctcgacg acaacggcaa ggttattccc tttaatgtga aaaagggcga
    18181 ccgcgtgctg atgccgaagt acggcggaac ggaaatcaag atggacgaca aggtgtacca
    18241 gatcatgcgc gaggaagaca tcctcggcat cctgaaataa attaaggaga atcagaaatt
    18301 atggcaaagc aacttaaatt tgacgcagcc gcgcgccagg cgatcctgag cggcgtggag
    18361 cagctcagcg cggccgtgaa ggtgaccctc gggccccgcg ggcgcaatgt gatcatcgac
    18421 aagaaattcg gatccccgac cgtcacgaaa gacggcgtgt cggtggccaa ggaagtcgag
    18481 ctgaaagatc cgttcgagaa catgggcgcc cagatggtgc gcgaagtggc cagcaagacg
    18541 agcgatgtgg ccggcgacgg caccaccacc gccacggtgc tggcggagtc catttaccgc
    18601 cagggcctga agaacgtcac ggccggcgcg aacccgatgg agctcaagcg cggcattgac
    18661 aaggccgtca ccgccgcggt cgagaagctc cagagcatca gcaagcgcgt gaaggacaac
    18721 gaggaaatcc gccaggtggc cacgatttcg gccaacggcg acgacaccat cggaaccatc
    18781 atcgcggacg ccatggacaa ggtcgggaaa gacgggacca tcaccgtcga ggaagccaag
    18841 ggcattgaga cctcgctgga cgtggtggaa ggcatgcagt tcgacaaggg ctatctgtcc
    18901 ccgtacttcg tgaccaacgc cgagaccatg gatgtgattc ttgagaatcc ctacatcctt
    18961 ctcttcgaga agaagatctc caacctgcag gaccttctga atctgctgca gagcatcgcc
    19021 aagtccggcc gtccgctcct gatcgtggcg gaagacgtgg aaggcgaagc gctggcgacg
    19081 cttgttgtga acaagctgcg cggcacgctg caggcatgcg ccgtgaaagc ccccgggttc
    19141 ggcgaccgcc gcaaggccat gatggaggat atcgccatcc tgaccaacgg caagttcatc
    19201 accgaagacc tgggcatcaa gcttgagaac gtcgagatcg gcgatctcgg caccgccaag
    19261 cgcgtgacga tcgacaagga caacaccacg atcgtggaag gcgccggcaa gacggccgac
    19321 attcagggcc gcattgcgca gatccgccgc cagatcgagg aaaccacctc cgactacgac
    19381 cgcgagaagc tgcaggaacg cctggccaag ctggccggcg gcgtggccgt catcaatgtc
    19441 ggcgcggcga ccgagagcga aatgaaggaa aagaaagccc gcgtggaaga cgcgctgcac
    19501 gccacgcgcg ccgcagtgga agaaggcatc gtcccgggcg gcggtgtggc cctgctgcgc
    19561 acgcttccgg ccctcgatga cctgaaggtc gaaggcgacc aggcgatcgg cctggagatt
    19621 gtccggcagg cccttgaagc cccgatccgc cagctggtgg aaaacgcggc ggtggaaggc
    19681 gccattgtgg tgcaggacgt gaagaagggc aagggcgccc acggctacga tgtggccaaa
    19741 ggcgagttct gcgacatgat caagtgcggc atcgtggacc cgacgaaggt gacacggtct
    19801 gccctgcaga acgcggccag cattgcttcg ctgctgctga ccaccgagtg catgatcacc
    19861 gagctgccgg agaaggaaaa gagtccgatg cccggcatgg gcggcgacat gggcggcatg
    19921 ggcggcatgg gcggcatgat gtaaatcagg ccaaaagccg tttgacataa cgaagcgggg
    19981 cgcatatagt gcgccccgct ttattgatag taatgggaga agtgttttga atacagaata
    20041 tctggaaaag gcaaaagaac gcatacccaa cgtgccgctg ctgatcaacc tgatctcgcg
    20101 gcgcgtgcgg cagctcaatg ccggcgaacg ctcgctcgtg aagcctgaca ccctgaacat
    20161 ggaaaaggtg gacattgccc tgaaggaagt ggctgagggc aagcttcagg ccgagatggt
    20221 gtttgaggcc gaccagacca cctcttccga gcgcgtgatt acgctctgac gcgtctccga
    20281 tccgcaatgg cccgttccac caggaagccc gccggaggcg gcccgctggc atccaaccgc
    20341 aaggcgttcc acaattacac cgtgctggag aagtttgagg ccggcatggt cctccgcggg
    20401 accgaggtca agtcagcccg ggcaggacgc atctcgatcg aggaaagtta tgcgcaggtc
    20461 gaagacggac agatcacggt ttatgaactg accatcatgc cgtacgagtt cggaacctcc
    20521 cacaaccaca atcccaagcg gccgcggcgc cttctgcttc acaaggctga aatcaggcgg
    20581 ctgcatgcgc agaccgtcat caaggggctt gcgctgatcc ccctaaaaat ctacctgaag
    20641 cacggccaca tcaagatgga aatcggcctt tgcaagggta aacagcttca cgacaagcgc
    20701 gacgcactcc gtgaaagaac cgcgaagctc gaagccgcgc gcgcgatggc cgcccaccgg
    20761 cacaggtagt cagccccggg ccgccagtgc ctgcagcagt tcggcccact gcgccgggga
    20821 aaggttctcg gggcgcattt ggtggtccag gcccagttcg acaagaagag attccatatc
    20881 ttcctttttc attccgcgcg ccggcatgat ctgcgacaac agagttccaa gctgttttct
    20941 ccgtttcgaa aagaattcat gcagcaggcg caggatgaaa tcgggctgca ggccgccggt
    21001 caggggttcc ggcctgcggc ggagccgaac gattgccgaa tccacgcgcg ggcgcggata
    21061 gaaacagccg tgcgacacca ctctctccac gaccgcctca tagaaggtct gcacacagac
    21121 ggacagcagg ccgtaggctt tgcatgaagg gccggcggtc atccgttcgg ccacatccct
    21181 ctgcacggtg accaccacga gctcgggccg cgcgggggcc gttatcacgc gcacaaggat
    21241 gcgtgtcccg caggaatagg gcagattcga aaccagtttg ccgcagccgc ccgcaagcat
    21301 accctccagg ggggcttcca gcgcatcctc cgccatgagt tcaaggtttg aacaggcgga
    21361 gagtgtctgc ctgagataat cgcaaagccg gaggtccttt tctatggcgg tcacgcggcc
    21421 tgcgcgcgcg agcagttcgc gcgtcacggt tccgagaccc ggaccaatct ccaggaccga
    21481 atcgctcttt ttgagctcgg ccagatccac gaagatgtta aggatattcc ggtcaatcag
    21541 gaaattctgc cccatgcttt ttccggggac catctgcagt tcctgaagga cggccttcac
    21601 ggcgaagggg ctggtgagcg gcgtcaggaa atcagtcatt tcttccgcca gggatttttt
    21661 ttgccggcca gatcgcaggc cagctcgagc gctttgagca tgctcccctc gcgggcgcgg
    21721 ccctttcccg ccagatcaaa tgccgttccg tgatccgggg aagtacgcag aatgggcaga
    21781 ccgagtgtga tattaatccc ctcgtccatg gccagcattt tgagggggcc cagcccctgg
    21841 tcatgataca tcgccacgac ggcatcataa gcgcccctca gggcgtgata gaaaatggca
    21901 tccggagcaa cagggccttc cgcgcgaagg ccggcccgac gggcggcgag gaccgcggga
    21961 tgaataatcg tcttctcctc ccggccgatc gcgccgccat cgcccgcgtg cggattcagg
    22021 ccgcacacgc cgatctttgc ccggcggcag cccagccacg gcagagcctc cgcagtgatg
    22081 cgtatggctt ccagaatatt ctttctggaa atgctctgcg caacgcgcga aagcggaata
    22141 tggcgcgttg ccagaatcac gcgcaggccg ccgcccatga gcatcatggc aaagcgtttt
    22201 gttcttgtga tttccgcaag atattcggta tggcccggcg ctttcagtcc ggccttcttc
    22261 aggccctcct tgcagatggg cgccgtgacc atcgcatcga aataaccgcc catgcagccc
    22321 tccgcggcaa gacggaccca gtgcatggcg tggcgggagg ctgaggccgt gatttttccc
    22381 ggtttccatg caaggcgcgc gccatccgga tcccatatgg agaagctttg cgacttaacc
    22441 ggcaggccga acttccgcgc ctgaagcgac agcatccccg cattcccaat aaggacaaaa
    22501 cggcaatttg aaggccactt gcatgccgaa agagccttta gtgcaacctc cggccctatt
    22561 ccattgatgt ctccggctgt gaccgcaatc agcttcattc aaaaatcttc acagtgtgct
    22621 ttttccgcag acgcgcgatc cattcccgat agattcgctc ttcctccgcg cttcgcagct
    22681 ccttttcgat ctggtcctta acctcgtcaa acgggcggac cgccgcctga cggcgctctt
    22741 caagcttcag gatgtacaga tcccgggaac cgacaaccgc actgactccg cccggctgga
    22801 gcgacttaag cgcttcggcg aaatccggcg caagatcccg aaccgccacc cagccccagt
    22861 cgccgccctg ctttgcgcgg ctgtcctgcg aatgctctgc agcaagctcc gcgaaatccg
    22921 cgccgtccgc cagctgtgcg cggaccgact ccgccaggga tcggctcccg gcgcggtcct
    22981 cttcggtttt tcccccgctg aaggttatca ctcgcagatg aactgcttcg ggtgttccat
    23041 accgatccgc gcgctcctcg tacaccctgc gaacagagga cggcgaaaca aaaatattgc
    23101 cccgcagtat ctgctggcgg gaaatcatga tgatcaggcg atcctgtatc tgttcccggt
    23161 aatcctcaag cgtcaatcgt tcggccgcca gctcggacag gaaagccccg cggtcattgt
    23221 cgaatttctc ccgaatcgtg gattcaagat attcgtccac cacccggtcc ggaataacag
    23281 ctttctgctg ctcaaaatcc tcgaggatca gggcctgatc aatcagcgca ttcagggccg
    23341 cctcaaaatc ctgcttgcgt ttctgctcca gctcggcccc ctcatacagc gtctgccgct
    23401 gagtctcgcc ctgctgcacc agcatcagca catcgctgac ggtcacgacg cggtcgttga
    23461 ccagggcggc aaaaccgtca gcaaccacgg gggcgcgctc cgcacccgcc accgggcggc
    23521 acagacagat gccgaacaaa aagaaaactg ttaaccgacg cggcattctc atttcacaac
    23581 ctcttcgcag tatgccagac cgggagggcc ggcgcaacgg gttttggccc tccgctgcta
    23641 tttttgactg gcaaacggcg gccgggcggt aataatccgc aggcattatg acaaaccata
    23701 tcagcatcgt gcttgccagt tcatcgccgc gaaggcagga aatgatccgc ggcctggggc
    23761 tcggctttca aatcatcaaa ccggatgtgg atgaaacgcc cgaacccggc gaaacgccgt
    23821 cggatttcgc gctgcgcgca gcccgcgaca aagcgcgctg ggtcgccgaa aaaatcatcc
    23881 gggacgggac catcgcgcaa cccgtcctgg tcatctccgg cgacacgatt gtcaccctgg
    23941 ggaatcgcat tctggggaaa ccgcgcgatg aagcccacgc ccggcatatg cttcgcgaac
    24001 tgagcgggcg gtctcacgat gtcatcagcg gcctgtgtgt gctgaaggcc gagaacaacg
    24061 ccatcgtgca cagcgaaagc atgaccgtca gcaccgaggt gaccttcaaa accctgagcg
    24121 acgaggaaat cgaggcctac atggccaccg gcgaggtcat ggacaaagcc ggggcctacg
    24181 gcattcaggg ggcctgcagc tacatgattg aatccattcg cggatcctat accaatgtgg
    24241 tgggactgcc gctctgtgaa cttgccggca ttctgatgca tgactacggg cttcatcttt
    24301 ccctggcgcc tcaaaaagcg aatgcataat gccaagactt cccttgaaga gaattgcgga
    24361 agcggcgctg atcattctgc tgtgcgtctc tgtccgactg acgggcatcc ggcacggcgc
    24421 gccggacctc atttttcatc ctgacgtggc caaacaggct ttcgtggcga gggacatcta
    24481 tgccggcgat tataaacccg gataccgcta cggagagaat tatcagctcg cgctgtatcc
    24541 ctacggcgat tccatcatgc tggctcatga gctgaagggc ctgacgccgg tgcttcacat
    24601 ggcgggtctc gcccgggaga aactgctgaa catgcatcgg tggaactggg cattcatgct
    24661 tcgcctgcac accgcggcgt ggatcacact ggcgctcgcc gttttcatca tgcgcgcgtg
    24721 gccggcgctg tcatggaaaa cccggctgcc ggccgccctt ctgctcatct tcgagccggt
    24781 gaatgcgcag tacagtcatt acggcatgaa tgatgtcccg ctggccgcac tgctgatgct
    24841 ggcatggctg gcggcggccg caatggacag ggacaccggc tgccggccgt ggagaagcgc
    24901 gctctgcggg ctgatcctgg gtgttgcctt cggagtgaaa tatcaggccc ttctgggaat
    24961 ccttcttcct gtcacggtct ggattcttct cttccgcgaa aaaaatgctg tctggcgctg
    25021 ggcggtggcc gctgcggtgg cgacgggctt tctggccggg gccctgtatg cctgcccccc
    25081 cctccgcatt cccgactatt tcatgaagca ctttccccgc ttcatggcat ggcaggcgaa
    25141 catcaccggc gaagaaattt cactgatggg aaaaatcagc cgcaacgcgg cccttctggt
    25201 tcgtttcagt ttccgctatg gatactggct gctgcttccg ccatgggttc tgctgatgct
    25261 ccgtgcagcc cgtaaaaaac ttgatcggaa caccctgctg atggcggtgc cgggattcgt
    25321 cttcagcact ttcctgcttt tccttttcat cgtcagccgc gatttcatcc gctcaaatga
    25381 cctggtggcg cttgcgccgt tctggatatg gcccgtggcg ctgatcaaaa gtccggaaag
    25441 gcgtggaccg cgcattgttt tccgggctat cctgaccgcg ctggtcatgg catggatatc
    25501 agcggcctgg cttgacagcc ttgccctgcg acggaccgac acgcgaatcc gtgctcgcga
    25561 ctggtgcgtg gagcattttc attcccggca gaccgccatt tttgaacgct acacccttcg
    25621 cacccggaag ccgcgcgttc aggaagttca ggtgcgcaat ctggtttcaa ggcaggcccg
    25681 acgccatatg cggcgaggtc aatatcaatg gcttgtgcag tcatccattg cgcgcgaccg
    25741 gtttttcgac ccgctttctc ctttctttga ccgagaagcc cagaagaatt atctggatat
    25801 cgaaaccgga catgtcctca tgaccgagtt cagggatcgt gaacttttct ttgcccaccc
    25861 ccgcatccgc atatatactc ccgccccctg atgtcagacc agttaaccat tttaagtttt
    25921 cccgtcggcc tggcggtcgt ctgcctgctg gccgagaccg ccgcccttct cggcctgcgc
    25981 cgcgccgcag gcggggaaaa gccggtatgg cccctccttg tgcgctggag cgccagacca
    26041 tggctggtct gggcggtatt cagcatggtt taccgtcttc agccggacaa ggctttcaac
    26101 aatgtgcgca cgttcccttt tttctcgaat ctctggcacg gccatgaagg ctggatggag
    26161 tcttttgttc gcatcgccac aacaccttcc gcctggatat ttgttccaat gaccctgctc
    26221 acattgacgg cgctctgcat gcttgcgagg aagatttcca ggcccgactc gggcgtgaag
    26281 acatggaaaa ccctctttgc ggtctatctt ctcggattgc tgctgatgct tgtgattgca
    26341 tcccttccgg acggaccgct tctaaaggaa aacgggcgcg cgggtgcgct gacgcgctca
    26401 tggcaggccc acaccaccgt gctctgggcg ctgccgcaca ttcgcaccaa aagtcacttc
    26461 ctgcgaaact ttgaagaaat ccagccgcgg ctgcgcggca ccatccacgg cctttctcac
    26521 ccgcccggag gcgcgctctc tctatatttt atcggcaaag tcatcggcgc ggaccacgag
    26581 gattaccgct cacacatgac gcgattccag tatctcattg gactggccct tttcagcttc
    26641 ctgaatgtgt ttgtgctgta cggcctggga tatctgcttt ttgatgaccg ccgaaccggg
    26701 ctggccgccg cccttttgtg gactgcggcg ccttccactc tcgcctattc atattttgcg
    26761 caaaatggac tgtatgcggt tttcttctgc cttgcgctgc tgttaacttg gacaacagtc
    26821 ttcaaatcgg atcggccgtg gcgctgggcc gtccccctgg gcctggtatt ctatctgctg
    26881 accatgatga actatgcatg gtgcattgcc acttccatct ttgccgtgtt cgcgctggcg
    26941 actggattgc agcggcgctg gcggccggcg gatatcctca tccgcgccat acttccactt
    27001 ggcataatga cccttctgct ggtctccacg ctcctctatt accggctcga ctatctggaa
    27061 atctacaatg tttccagcga ctatgttgaa cagtggtatc gcttcacttc cccataccgg
    27121 tggcttgccg cactgttagg cggacagatt gacatgtggc ttatgatggg cagcgcaacg
    27181 tgttcgtttt tccttgccgg cgccggagcg gcgctccgca ggaaaccact ggaacccgcg
    27241 acggtctttc tgctgatcat tctgggtgtg ttcgcgctcc ccctgctctt cggaccgaac
    27301 cccctcaaga tggaaaccgc ccgctgctgg aactggatac tggccgtgcc gctggctttt
    27361 gccgcaaacc ggatcatgca gctggaaaaa gcggaagttc tgcttccggc cgtcacggca
    27421 gtgagcggca caacatacct gctgatgaga ctctacctgg acttcgcgcc gtaggccgcc
    27481 cgcgctacac gcgaaacacc gcgccaagtt ttttgccgag gagcaggctg cttcccacga
    27541 gcgtcgcggt caggaatgcc atggcccaca cgcccagcgc ggccgccaca tatttgcccg
    27601 tgcccagcag ctggaagagc tcgaatatcg ccttcgtgat cggcatgtaa tccgcctgct
    27661 gagccagcat caggctgtcg ctgacctcca gcatgctgaa cgcaaaggtc agcagcgtcc
    27721 cggcgatcag atttgccatg atcaggggaa gcgtaatccg ccggagcgtt ttggccggcg
    27781 acgccccgag attccatgcc gcctcttcaa acgtcacgct ggtctgctgc agaccggcga
    27841 cggcgctgcg caccatatag ggcagacggc ggaccgaata ggccagcacc agaaacaggg
    27901 tcgggtttgt gcgcacatcc accagccggg gccaccagtc cgcatccgcg aagcctttca
    27961 gattgctgag ccaggagctc accgccagaa atccgaacgc catcaccagt ccggggaccg
    28021 ccagcgggat catcgaaagc gcgtccagca cgccgcgcag gcggatcttc gaacgaacca
    28081 ccacaaatgc gatggcaatg cccaccacaa tatcaaaagc caccgccagc gaggagaaca
    28141 gcaggctgtt gcggatgctg ctgacggtca tgtcatgtcc cagcgcctcg atgtagttgc
    28201 cgccggtgaa ggtttgcggc agcacgctct ggtaccagct gccgggggcg gcgaagctgg
    28261 tcagcaccac acccagatgc ggcagcagcg cgaggcccgt caccagcgca aacgggagcg
    28321 cggccagcag gccgcgccag ccggacaggg tccgcacggc cgactgtgtg ctcgccttgc
    28381 tctgcatcgc ataggcccgg cccccaaaca gccggcggct gacggcgtag agccccacgc
    28441 tggccgccag catcaccgtc accagcgcat aggggaacgg attcgatccg atttccttca
    28501 gcgaatcgta aatctgcacg gatgcgcacc gggtatagtt catgatcagc ggtgtgccca
    28561 gctcggtgaa gctccagatg aaaatcagcg tgccgccggc aaagagcccc ggcatcatga
    28621 gcggcagcgt gatccggaaa aagcggcgca ggcgtgtgca gccgaggttc gcggcggcct
    28681 cctccatggc cggatcaatg ttggccagcg ccgcgacggc attgaggtag agaatcggat
    28741 aaagggacag ggtctggagc agcaccactc cggcgtactg ccccccgccc agccagtcgc
    28801 gggcaccgag gccgaagagg gcgttgatca ttccgtaggc cccgaacatc tgctgaaacc
    28861 cgatggcccc gacaaacggc ggcagaatca tcggaaccag caccaacgcc gaaaacgccg
    28921 ccttgccgcg gaactcaaac tgattggcaa tccacgccag cggaagcgcg accaccatgg
    28981 ccagcagcgt tgtccccagt gcaatcttga ggctgttgaa cagaccttcc acataaatcg
    29041 ggttctcgaa aacgccgatc aggtagcgca gggtgaactt cccgttcacc cagaatccgc
    29101 ccgagatcac ggtgccgagc ggccagaaga aaagaaccga aagcagcgcc gtcaccgcac
    29161 cgaaaacaaa ccatgccgtg ctcttcctca tcgccgggtg cccccttcct ccctcgcgag
    29221 acgcgccgcc tcggcatact gcgcccggta ctgaatggtc cattcgcgca gcagatcaat
    29281 ccggtcatat tttctgccca gggccaggcc cgaagtccat gtcaggggtt ccggctccag
    29341 cggaaggcgc tgcagcgcat ccatcgcgcg cgggcacgct tcgggcccgc cccgggccac
    29401 gatttcgccc caggccatgc ggagctcctt tcccgcgtcc atgcacatgg cgcggaccaa
    29461 atcacgaaac aggccgaaat gcccggccgt ccagcgcggc cgatagacat actcctccgc
    29521 cagccggtag gcatccagcg agggccggct catgtcttcg gtggtatgcg cccgatgccg
    29581 ctcgtgggcg gcctgaaaaa cgggattatc cgacgggtag aaatcacgcc ggatcgggaa
    29641 ccgctgcagt gcgtacttct gcgggccgcc cggttcgcca acgcggtagc accagagttt
    29701 ctgcccctcc tcgctcagca ggtactgtat aaaccgctgc gcgatctccc ggcggggggc
    29761 gccgcgcagc agggaaatgg gatcggaact cactccggat tctccgcgcg gagtcacata
    29821 ggccatggac tcacggccgt gtcccgcatt gctcacctgc gcttcgaacc ggccgtaaaa
    29881 atcaatcgcc aatcccgccg ccgcattgcc catgccgaca tccagcggaa ccttgctggc
    29941 gctgtcggtg aagtagcgcg cattggcgcc gatcttctga atcagccgca ggccgttcat
    30001 ccagcccgtc tcgacagcct gctggtattc ctccgggaca ccttcgggca tttgtcccgg
    30061 cgccagtttc gcggcggcga tccgcaactc atagtcgtca atcatcggcc cgaatccggc
    30121 cgcttcaacc gcgtggcggc actggacctg cacgatggtt tcaaacgcct tggcgatgct
    30181 gccgctcttg gtcgggtccg ccagcccgag ggtgccgaac cagcgcggat cggccaggtc
    30241 cgcccagtgc tccgggtctc gatccatccc ctgcgcgagc atccggtcgc ggttgtagca
    30301 gatgccgaag gtgctcagtg tggtcccgta ccatgtcggg gtgcgccaca cctccccgct
    30361 catgcccgga ggaatcagtt ccgcgcccgc atccgtcgcc agcagtccgg gaggtatctc
    30421 ctccggagcc cacgccggga caagcagtcc ctgacgcgtc gcgctgtcgc cgtcataggc
    30481 gcccccgccg aaatacatgt caatccggct ggaaaacgcc tgcggatcgt ccgtgccgcg
    30541 gaaagccgca tacatgctgc actgctccgc ccagtccgcc tcgtccacgt ccccgggttt
    30601 cgcatcggga ttgaaggatt tgctgaggat gatggccgcg ccgtcaccgc gccatggccg
    30661 gccctgcgcc gtccaccagg cgcggaagct ggagacaaac tcgctggtca gataacgcga
    30721 gatttcggtt gtcccgccga tgttgcgcca gtccaccttg accggcgagc cgaaattttc
    30781 cgcatgccag cgcgagaatc ccagcgcata ttcgtgacgg atcgcctcgt tcatcggggt
    30841 gatgaccaca agcaccggat cgccctcccg ccattcgcgc accccggtct cctggcggaa
    30901 gacaaacggc agcgccacca cggcggccag caccgcaaga atggaaagat tcctggtcat
    30961 gccttcaaaa cctgaacgtc ggccggatcc acatagagcc ttgtttcggc gcgcgcgtca
    31021 tcccgggcaa ccagcctggg ccgggtttcc agcgccttca gggaaatatc gccgcccagc
    31081 tcgacggcat gctgggccac ttcccccaga tagatggtgg cgtgaagcgc cgcattgaac
    31141 acgttgggac catccgcgcg gtcagtatgc agctggacag cttcgggacg aaccgacagc
    31201 cagacggttt ctcccgcctc aagcccgccg gcggtctggg ccgtccatat ccccgccgcg
    31261 gtttcgatct ccgcccggcc gtcgcgccag cccaggacgc ggccttcaac aaacgtggtc
    31321 tcgccgatga actgggccac aaagcgactg ccgggccgcg tgtagacctc ctgcggagaa
    31381 ccgatctgaa gcaggcgtcc ctgatccagg acagccagac ggtcggcgat ggaaagcgcc
    31441 tctttctgat catgcgtgac atagatcgcg gtcagaccgg cctccttgca gatgcggcgg
    31501 atttccgtgc gcatttcgag gcgcagcttg gcatcaagat tggaaagcgg ctcgtcgagc
    31561 agcaagcact gcggctcgat caccagcgcg cgcgccagcg cgacgcgctg ctgctggcct
    31621 ccggagagtt cgttgggttt ggaatcggcg cggtcggcca gccggaccat ttccaatgcg
    31681 cgcatcacgc gcgcctgtat gtcggctttc ccgatcccgc gcatttcaag gccgaaggcc
    31741 acgttttcgc gcagggacat gtggggccac agcgcgtagc tctggaacac catgcccatg
    31801 tcccgctgat gagggggcag atgcgtcaca tcgcggtcgc cggccagaat gcggccctcg
    31861 tcgggatcat tcaggcccgc aacggcgcgc agcagcgtcg tcttcccgca tcccgaaggc
    31921 cccagcagga agaaaatttc gccggcggca atctgcacat caatgtctct gaggaccgtc
    31981 tttgcgccaa agcgtttcgt cagcttctca atcgtaatcg gcaccggcat gtctgcttct
    32041 ccttttgacg ccctgttttt gcacatagta aaacatcaga ggaggttgtc acgggatatt
    32101 ttcgcgtggg ggttcaggag gatgcggccg ggggcgggga aaaagttcca atcattggaa
    32161 gttttcccgc aaaaagttcc aaccattgga agttttttgt cgaaaagttc cagggattgg
    32221 aacttttcac gccttgcgca gatgggacgg aagtatcttg agctcctcgc ggtacttggc
    32281 gaccgtgcgg cgggccacgt tgaagccgtt ttccctgagg gccccggcca gcgcctggtc
    32341 ggacaagggg tgtgacgggc tttcctttgc aaccaggttc gcgagggcct ccttgacggt
    32401 cttgtttgaa acctgctgcc catcggcatg ggcatagccg gaagcaaaga agtacctcag
    32461 atcataggtg ccctgcggcg tggcgacata ttttccggag atcgcgcggc tgaccgtggt
    32521 ttcatgaatt cccagttcgt cggcgatggc cgacatggtg agcggccgca gatgcgaaag
    32581 gccgtgctca aagaaatcgc gctgggtctt caccagctcc ctgactatgc ggtagatggt
    32641 ctgctgccgc tgattgatgc ttttgatcag gaacatgccg ctccggattt tttcctgcac
    32701 gtagtttttc acatcggccg gggtggccgg atcctccagc agcttgtgat agtgacgact
    32761 gatgcggatg tgcgggagat ggtcgtcgtc aagctgcacc acgtactctc cgtccacttt
    32821 ccggatggtg atttccggat ggatgaagtt ggtggattcg gcggcgaagg cccggccggg
    32881 cttgggctca agggcggcga tcacacgggc cgtctcctgc acggtctcca gcggcaggtt
    32941 catttccttc gcgatgcgcg cgtattttct tgccgccaga tcgttcagat ggagccgaac
    33001 catgttcgcg gccggcgact gatccaggcc gcgccgccgg agctgcagca gcaggcagtc
    33061 gctcagatcg cgggcgccca cgccgaccgg gtcaaaaccc tgtatgaccg ccagcacctc
    33121 gttcagtttc tgcacggagt atcccgtgga ggcggcaagt tcctcgatgc tggtggacag
    33181 atagccgtcg ctggtgatac tgccgatcag aagctctccg attcttcggt cctcctgctc
    33241 cagccccgcc atcggaagct gggccatgag atgctcctgc agagattctg actgcaccat
    33301 tgaatccaga aaatgctcgc ggcgctccgc gtcatccggc gtccagcgct ggagggtctg
    33361 gctctgctga tagaactccc gccagtcgtc gtccagcttc aaaaggattt cgtagtcctc
    33421 cttgaagtcc atttccttgt tgttgtcggt ttccggctca ggggcgggcg gttcggcggg
    33481 ggtctccgga gccgcctgag gggcgtcata ttcctccagg gccggattct gctccagttc
    33541 ctgctggatc accgactgca gttcgagcac cgggagctgg agaatttcca gcgactggcg
    33601 gagctgcgga gccagtatca tctggagccg ctggtcctga acctgactga gaatctgctg
    33661 agccattacc gtgcctttga ctggacatga tagctttgtg aagtatgcta aacaagatca
    33721 ttaaccgggg ttttgcatga gagagcgtgt gtgtgtactg gccagcggga gttcggggaa
    33781 ctgcaccttt gtgtcggcag acggggtgtc cattctcgtt gacgccggca tcagcggaag
    33841 agaaacggaa cggcggctgg aggagatcgg tgaggatccc ggccgcctgc gggccatctg
    33901 cgtaagccat gagcatacag accacaccgc gggcatcggg gtcctgcacc ggcgctatgc
    33961 cacccccctc tttgcaaaca gcggcaccat taacggaatc ggccggcagc ggggcctggc
    34021 cggcctcccg tggaatgttt tcacaacggg gcagccattc cagatcggca cgctccagat
    34081 cgaaccggtt tctgtcccgc atgacgccta tgatccggtc ggtttcatta tccgctcctc
    34141 caccgcggcg ataggcgtgg tcaccgacat gggcatggcc accgccctga tccgcacgcg
    34201 gctcagggac tgcgatgcgc tggtgatcga aaccaatcac gatgagacca tgcttcaaaa
    34261 cgcccagcgg ccctggatgc tcaagcagcg cattatgggc cggcagggac acctctccaa
    34321 ccggcaggcg gccgccgtca tcgaggagat cgccggcccc cgcctgaagc agatatttct
    34381 ggcccacatc agttccgact gcaatgaggc gtggcgggcc gagcaggaaa tccgccagac
    34441 cgttgagcac gccgggctgg ctcatatcca gattgcctgc accttcccgg accggatcag
    34501 cgccgtctgg cagccggcct gaatcccttt gacacccgtt ccggtgcggt gttagcgtag
    34561 gcatggactg ggactgttgt ctctgggagt tggtgatcaa tggcaaaagt tattcttgag
    34621 aaggtctgca aggtgtatcc gggcagcgtc acggccgtga gcgatgtgaa cctggaaatc
    34681 gccgaccggg agttcgtggt gctggtgggc ccttccgggt gcgggaaatc caccacgctg
    34741 agaatggtgg ccggactcga ggaggtcagc agcggaagca tttatattga tgaccgcaaa
    34801 gtcaacgacg tgccgcccaa ggatcgcgac attgccatgg ttttccagaa ctatgcgctc
    34861 tatccccaca tgacggtgcg cgaaaatctc gcctttggcc tgaaactccg gaagatgccg
    34921 cgggcggaca tcgaacagcg cgttggcgag gccgctgata ttctgggcat cgccgacctg
    34981 ctggagcgca agcccaaggc cctctccggc ggccagcgcc agcgcgtggc cgtgggccgg
    35041 gccatcgtgc gcaagcccaa ggcctttctg ttcgacgagc cgctttccaa tctcgatgca
    35101 aaaatgcgcg tgcagatgcg catggagatc agccgcctgc acacccgcct ggcctgcacg
    35161 atgatctatg tcacccacga ccagattgaa gccatgacca tgggcgaccg cattgtcgtg
    35221 atgaaggacg gcgttgtgca gcagacggac cggcccatga ccatctattc tcagccggcc
    35281 aaccggtttg tggcgggatt catcggcagc ccgcccatga acttcttcga cgggcggctg
    35341 gaaaacaggg acgggcgcat gttcttcaac gccgggacgt tcagcgtgtg cgcggaggat
    35401 tcccagtacg cgaggctggc ggagcacgac gggaagaaga tcacgatggg cctgcggccc
    35461 gaagacctga aggacgcgct ttacgctccg caggcttccg cggaccactc cgttccggcc
    35521 ggagtggagg tcgtggaacc catgggcgcg gaggtctatc tttacctgaa caacggaccc
    35581 cacaacttca ccgcccgaac cgatgcgcac gaccccgcca ccgcgggcca gagcatcacg
    35641 atggtcttca acatgaagaa agctcatttc ttcgacgccg aaaccggccg aaccattctt
    35701 taaagggaga cgaccccgtg ggcatgatgc tggatcagat aatgaccgtg atcggccaga
    35761 cgctgattgt cgtcgcggtt tctttcatca tgctctgcct gcttgtcagc tatgcccgtt
    35821 ttcatctgct cttcacgggc cgccggccca gcgcggcggc ggcagaagga gtgctcgatc
    35881 cctttcgcgc atgcatcctg aaacaactcg gaagcatata caggaatccc gaaccctttg
    35941 ccgtgatgac actgcgcgca acggacggcg gccaccggcc ggaagacccc gtcgcccgga
    36001 ttcgcgggat gatccgtgcc tcggatatgg tcatgcgcta tgacgaaaac caaatcggca
    36061 tgatcatcaa gaccagccgg aaacatgcgg aacgggtggc gcagcgcgtg gcggacggac
    36121 tgccgggttc gccggcgatc ggcattgcca cctgtccgga aaacggcacc cgcctgagcg
    36181 acctgctgga aaacgcggag catgccgcag aagaagccgg ccggctgagc ggcggagtga
    36241 aactggcagg agaacccgtg accgcacccg acgaaggaga catcctgccg gcgcaggaaa
    36301 aatatctgga tgatctcacc ggcgtgcttc gcggcgacct gctgggccgc acgctcccta
    36361 aatttatttc ccagcaccgg aaagaaggcc gcaccgtggc catgatctac tgcgacgtgg
    36421 acaactttca ggattacaac cagcattatt cccgggaagc cggcgatgca ctgcttcagg
    36481 gcgtttcccg gcttttccag gagaatctgc gtgaagacga tctcattgcg cgctacagcg
    36541 gtgatgaatt tctgatcctg tttcccgccg cagccgccga tgcacagcat accgccagac
    36601 ggctttctgc cgtcatcaag cgggccgaat tcaagtatgg cgcgatcacg ctgaaaacaa
    36661 ccgtgagcat gggcatcgct ctcgtccccg gtcatgcccg cactctcagc cggctttttg
    36721 aggcggcttc gcatgcactc catgccgcca aacagaaagg cgtgggattc tgcgtgatgt
    36781 atgccccggg catgggggtt cagtcccgca cacaggatgt gattgaaaaa ttctaaggaa
    36841 ctgaaacatg aaactgggcg taaacattga ccatgtggcc accatccggc aggcgcgggg
    36901 aacccgctat ccggacatcg tcgaggcggc cgaggcctgc cgccgaggcg gagcacacgg
    36961 cattaccatc catttgcgcg aagaccgccg gcacattcag gatgccgatg tcttcgccgt
    37021 aaagaaatgg ggacgcctcc cgctgaacct ggaaatggcc aatgccgaag agattgtcgc
    37081 cattgccctg aaggtgaagc cggaggaagt ttgtcttgtt cccgaaaaac gccgcgaact
    37141 gaccacggaa ggcggactgg atgtcgtaag agacagaaag ttgaagcaga ccgtggcccg
    37201 cctgtccgaa gcgggcgtgc gggtcagcct gtttgtggat cccgatcccc gccagctgga
    37261 agcttcattg aaggtcggcg cgacctgcgt ggaactgcat acaggacggt actgcgacac
    37321 ccggggaacc atccggaaac gcgagctgcg gcggctggcc gctggcgcaa aactggcgca
    37381 caaactgggt ctccaggtca atgccgggca tggtctcaat ctggacaatc tggacggcct
    37441 ggccagtgtc ccgcatctcg acacgctgaa cattggacac agcatcgttg cgcgcgcggt
    37501 cctgatcggg ctggagccgg ctgtgcgtga aatgctcagg gccatgaaag gaatccagca
    37561 gcatgccggc aggtaataca gatccgcgcg gagtggcggg catcggcatt gatatcgtcg
    37621 aaaaccggcg ggtcgaagat gcccttgccc ggcacggacg ccgctttcgc aaccggattt
    37681 tccgcaaatc agagcaggac tactgtgacc agaaggcccg tccggcgctt cattacgcca
    37741 gccgcttcgc cgtcaaggaa gcggtcgcca aagcgttcgg caccggcatc ggcgcggaac
    37801 tgggctggct ggatatcgaa gtggtgcggg acggcggcgg ggcgcccgtc gtgatgatgc
    37861 tcggcaaagg ggctgaactc gccaggaaga tgaacatcga caggatctat gtcagtcttt
    37921 cgcacacgca tcagtatgcg gccgctacgg ccctgctcgc tgtaaaatgt ccgcgggggc
    37981 ctgcatgatc atggtctcat cagcccagat gcgcgaactg gaccggcgca cgattgagga
    38041 ggcgggagtt ccgcaggtgg tgctgatgga ccgggccggg cacgccgtgg cggatcacat
    38101 ctgctggctg ctgcgggcgg ccggaatcag ccatccttat atccgttttc ttgcgggacc
    38161 gggcaacaac ggcggcgacg ccatcgtcgc cgcgcaatat cttcagaagc gaggttatag
    38221 aacagatatc tggacgtttg gacgaccctc ggaagcttcc ggtctggaca ttcacattat
    38281 cagcggcgag gcggattggg aacaaatttc cgccggcgca tttatccccg acctgattgt
    38341 ggacgggctt ctcggcacgg gcaccaaagg cgcgccgcgc ggactgatcc gcaaggccgt
    38401 cgagtggatt cagagcctgt ccggcaaaac cctgatcgtc gccattgaca tccccacagg
    38461 actcgatgcc gacacaggcg ctgcaaatga tccggccgtg cacgccgatc tcacgatcac
    38521 attgggcgcg cccaaaacgg gactgctccg gccggaagcg ctttcctgca tcggctctct
    38581 tgaagtggcg gacctcggca taccggaaga atatttccgg gccctgaata aaccggccgg
    38641 ccccgacctc atctgcgcga acaatctgcg cgcgctgttc ccccgtcgca gggctgacgc
    38701 ccataaagga ctgttcggcc atgcgctgct gatcggaggc tctccgggtt tttccggagc
    38761 gatctcgatg gcggcccgcg cggccgtaaa atccggtgcg ggacttgtat ccgccgcggt
    38821 gccggaaagc atcgtttcga tcgtggcccc ctcctgcccg gaggcaatgg tgcatgcgat
    38881 ggaactttct gacagccttc tggaaaagtg cacatctgtc ctgatcggac ccggtcttgg
    38941 gaacaatccg gaagctgcaa ggctggtgga acggatgctg aaaacgagtt ctgttcctct
    39001 cgtgctcgat gccgacgcaa tcaatgcgct ttcgggacgg ccggcaatca tcaagaagga
    39061 aaaatgcccc gttgtgatga ccccccatcc cggcgaactg gccggactga tgcaaaccac
    39121 cgcggccgcc attcaggcag accgctgcaa agcctgtctc gccgctgcag aaaaatccgg
    39181 ggcaatcgtt atattaaagg gggcgggaac actgatcgct gatcccgcgg gagaactctt
    39241 catcaacctg aacggcaatc cgggaatggc gacaggggga tcaggcgacg tgctggcggg
    39301 cattctgaca ggcctgcttg cacagggcat cagaccgctt gacgccgcaa aagcggccgt
    39361 ctggattcat ggaaaatcgg gcgatgcggc cgcgctgcgc cggacacaaa cggccctcag
    39421 cgccgcagac atcatcaata atatccccag tacttttcgc cgtctgaccc caaggtagga
    39481 aactggagcc agctcccggg gtcgaaccgg gaacctgctg attacaaatc agc
//
LOCUS       DAOQWR010000004        39050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1204_length_39050_cov_4.888011, whole genome shotgun sequence.
ACCESSION   DAOQWR010000004 DAOQWR010000000
VERSION     DAOQWR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 39050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39050
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_39050_cov_4.888011"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKK36_00695"
     CDS             <1..456
                     /locus_tag="PKK36_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR93107.1"
                     /translation="WFNLPLWGRQYAAVVNFRFAQTLSLLLDGGVSLVEAFPLAGRAT
                     GSPWVTDLVDREGESLSRHGSSLADAVRRIAPLNDVLPGWIQAGEASGALAELLSRAG
                     RRYQLQWQRMIGRSLEVLEPALIILVGGLVLLLALAVLTPILSLNSSLM"
     gene            complement(374..1213)
                     /locus_tag="PKK36_00700"
     CDS             complement(374..1213)
                     /locus_tag="PKK36_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNR93108.1"
                     /translation="MTRGFQAAAAVLLLLGCRPASVHVPSAETPEVMAAVVHTFPHDP
                     RAFTQGLVYYNGFLYESTGIYGRSSLRQIVPETGEIVRKIMLPHSVFAEGLALCKGRL
                     IQITWHAETGYVYDAETLTMLRTFRYKGEGWGLCCDGSRLIMSDGSSTLTFLDAETFE
                     PSGRIEVTDRGQPLSLLNEMEYVNGLIYANVWKSDRVACIDPQSGSVVRWINCEALRP
                     RSPMRTEDVLNGIAWDADGKRLFVTGKLWGVLYEITSGNCSATEWESEQPAPEAAPGR
                     PPE"
     gene            complement(1220..3199)
                     /gene="tkt"
                     /locus_tag="PKK36_00705"
     CDS             complement(1220..3199)
                     /gene="tkt"
                     /locus_tag="PKK36_00705"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNR93109.1"
                     /translation="MNTQLTADTIRGLAMDIVQKANSGHPGAPMGMADIASVLFLKHL
                     KYNPQDPRWPDRDRFVLSNGHASSLLYSLLHLAGYSVSMDDLKAFRQWDSITPGHPEN
                     DLTPGVETTTGPLGQGGGNAVGMALAEAMLAARFGSEIVDHYTYVFAGDGCMMEGITH
                     EVFSMAGHLRLNKLILVYDDNRITIEGKTDLAYSDDVRRRFEGYHWNVLSIDGHNHEE
                     IDQALTAAKKEKDRPTIIIARTHIAKGSPNKQDTSGSHGSPLGDAEIAATKKLLGLPE
                     DKFFHVPDEVRSVFSERKQQLSNDYLQWHKRCAEFRNADPALAAQWDAAQEGSIPANL
                     DKILPKFSPDKAMASRQASGAVLQELAKAIPSLVGGSADLAPSTNTELKGFDSVQAGC
                     MTGRNLHFGIREHGMAAIMNGMALHGGFIVYGATFLVFSDYCRPAVRLSALMHLPVIY
                     VFTHDSVFLGEDGPTHQAVEHLAALRAIPNLTVIRPGDAAETAQAWAAALRNRHGPTA
                     LILSRQSLPTLDRSVCAPAAGLQQGAYTLWQNRDGAPDLIILATGSEVAPALKAAQSM
                     EKESAAIRVVSFPSWELFDSQPNDLKEKILPAACAKRLVVEAGVSHGWQKYAGSAGAM
                     VCLDRFGASAPAEVLAEKFGFTAEHIAQTARQLLA"
     gene            3361..4209
                     /locus_tag="PKK36_00710"
     CDS             3361..4209
                     /locus_tag="PKK36_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR93110.1"
                     /translation="MIDLHMHSSFSDGSFTPEELVNKAVELNVSAISLTDHDTMAGVP
                     RFLEAGARQNLQVIAGVEISTDYKGRGMHILGYGMNIANPELQQSLDWIRAGREARNM
                     EILEKLNRLGFIMTWEEVVSRAGEDVVGRPHFAQTLIDKGYVKDKYEAFDKWLGHGKP
                     GYADRRRLDPEAGIRIIRKAGGVAVLAHPFTLNLSNTGLHSLLLELSRAGLEGIEAYY
                     AEHKPDMQDLYLKMAAEYDLLVTGGTDFHGSLTPMLRIGFGFGSLNVPDSLLEPLLAR
                     ISSRAG"
     gene            complement(4216..4761)
                     /locus_tag="PKK36_00715"
     CDS             complement(4216..4761)
                     /locus_tag="PKK36_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93111.1"
                     /translation="MNQFLRAESGEGIFWTIMIIIWIVSKATAASRKANKKAPPPAPR
                     VSKPKLPPQELQAFLDSLTGGRETRPRATVQIPTERRSAPSSRRIRRESPTVHKPPAA
                     QPAAVAEVQIGRQMSLLNTSVRDLITDIPSARMPNLSIGFFSPQSRNAPREKPNLRNH
                     SELRKAMLGHVILSRPPSAGW"
     assembly_gap    4887..4896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4897..5834)
                     /gene="floA"
                     /locus_tag="PKK36_00720"
     CDS             complement(<4897..5834)
                     /gene="floA"
                     /locus_tag="PKK36_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262252.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNR93112.1"
                     /translation="MPIIAAVLVVVALIIIFTFIYFLKVWIRALMSGARVSIFNLVGM
                     KLRGVPPTLIVDARIRAVKAGLPLTSDQLEAHYLAGGNVIPVVQSLIAADKANIELAF
                     QRAAAIDLAGRDVYDAVRTSVNPKVIDCPDPAKGATMLDAVAKDGIRLLVKARVTVRA
                     NLDRLVGGATEDTIIARVGQGIVSAIGSSTTYKDVLENPDHISRKVLNSGLDAQTAFE
                     IVSIDIADVSVAGVSGAREVANVGALLETERAEADKKLRQAEAEGRRAMAIASEQEQR
                     ARVQEMQAKVIEAQAQVPLAIAKAFEKGNLGVMDFYR"
     gene            complement(5847..7049)
                     /locus_tag="PKK36_00725"
     CDS             complement(5847..7049)
                     /locus_tag="PKK36_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNR93113.1"
                     /translation="MRLLRLIPAAVLLTALQAGAAIDFRSTNEYRLAGNELQEGQLWL
                     AAVTTEVRGAVSDDFHALAQTALLGGTFSGSVWCAAETADFSGTAQGHARLLAAQQVT
                     VRGRIMRSAMSASGTSIYITPEGVVDGDAVLASKRIVVEGKIGGAAQLIGDEITLAGT
                     IEGPLRIIGSDIAVMPSAVLRGDIVYSSSKELFLDPRVKHEGLLVRKPLTQPASSSSA
                     MPWALRLQLRVVQFMGALLVAWVWLRLAPLALSRAAHQLSVAPWRCFFTGLAVCFLML
                     PVILLLAISLIGIPLALLGTGIFAILLYVSRIVTAVLLGRIVLRSRGGKTPASPLLII
                     TAGLLCLYILTLVPKISGGIWIVAVCAGLGALVLNVAAGSIIYVQPPGAKPPQEISQT
                     NTDSSKTE"
     gene            complement(7046..7522)
                     /locus_tag="PKK36_00730"
     CDS             complement(7046..7522)
                     /locus_tag="PKK36_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNR93114.1"
                     /translation="MMLFLTLLFCGFLLLAVEIFVPGGVLGTLGAVALFGAVISAFAV
                     FGPQTGFLVAILMLILMAVGLVLWIRIFPNTPIGRALTLSKDGQSFKLDSEANPPLVG
                     AEGSAQTDLRPAGIAVINGRRMDVVAEGEWIAAGTPVRVISAKGNHVTVRRKGEPG"
     gene            complement(7536..8939)
                     /locus_tag="PKK36_00735"
     CDS             complement(7536..8939)
                     /locus_tag="PKK36_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNR93115.1"
                     /translation="MKKICILFLICAASAPLPLPAAVESPPGPVYVIPVEGMIEPALL
                     YVIRRGVAEAEQRKARAIILQMDTPGGTIQAAEEIIRILEHVSVPTYTLVDRNAISAG
                     AIIAMATDHIYMTPGSKIGDAMPIMMSPFGGVQSLPDGTEEKMLSYVAGLIRATAQHK
                     GHNDKLAEAMVRRELEYKIGDEVISPEGQLLTLTSAEAEKKYGEDQRPLLSAGTVDSL
                     ENLLKLIGMDNAEVRELGITTAERIARFIAALGPVFLIIGMLGIYIEVKTPGFGLPGI
                     TGVLALAIFFWGHHIAGLAGMEDLLIFLAGVLLLLLEIFVFPGFGAAGLLGLLCIGWA
                     LLNAMVQHYPGGPWYPDPNLLYIPFLKLTFSLIGSVVIAMLLGRWISRHPATSRLVLA
                     EDTSHDKGFVSSSNRPEELVGLQGRALTDLRPGGTGMFGERKLDIVSRGNYIEAGSAV
                     RIVETHGLQVIVEPLES"
     gene            9018..9734
                     /locus_tag="PKK36_00740"
     CDS             9018..9734
                     /locus_tag="PKK36_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR93116.1"
                     /translation="MNLKNRRILVTGGAVRVGHGIAAALAAAGAVPVIQYHQSGAAAD
                     ETVAELAGQGYSAQAIKADLSYADECERLISDAGPLDGLVNNAAIYLPDTLREFDPEE
                     SHRQLQINAIAPILLMKLFAQQCKSGAIVNVLDRNIRRPDPRYFSYSLSKKMLYEATL
                     QAACDLAPGITVNAVAPGPVLPPLHVKEPAGALMLKHRPTVEDVGAAVVYLMSAPAVT
                     GQTLFVDSGQHLGLTAVDAS"
     gene            9765..10700
                     /locus_tag="PKK36_00745"
     CDS             9765..10700
                     /locus_tag="PKK36_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HNR93117.1"
                     /translation="MSYDLTSLLVVAISSTALFENRAEHDLYLNKTLDDYIDHQISRE
                     NEPFAAGAALPLIRALLRLNELTPGRRLVEVVILSNMEPEVGVRVMNSVEHHKLDITR
                     AAFIGGEPVAKYLAAYNVKLFLSRSETDVQEALDCGVAAGLLYDPPARREEDDMKQLR
                     IAFDGDAVIFSDDSERIYQEQGIDAFYRHEKANAENPMSAGPFAAFLHAIAEIQKECR
                     RQGGEAPIRTALVTARSSPAHKRVILTLRAWGIRIDEMFFMGGVSKDQILKQFNPHIF
                     FDDQHAHAGPASEFVPSARVLTPRRPKNPAGGTHG"
     gene            10693..11046
                     /gene="folB"
                     /locus_tag="PKK36_00750"
     CDS             10693..11046
                     /gene="folB"
                     /locus_tag="PKK36_00750"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNR93118.1"
                     /translation="MDKIIVREIAVQCIIGVHPEERARYQRLLISLELEADLMPGIEN
                     DNILDTLNYQAVCEEVASFVTASRCWLIESLAAQIAAKCLEFPRVLAVTVTVDKPEAL
                     PGTSGVSVCIHRRRP"
     gene            complement(11048..12523)
                     /locus_tag="PKK36_00755"
     CDS             complement(11048..12523)
                     /locus_tag="PKK36_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNR93119.1"
                     /translation="MGMNRGIIITMLVVLPACVMTAGAQDDAAAPHIEVIGTRVTFPV
                     YDLQTAAQIGKPLRLYQELTNAYIVKVEQPDGSYALGAFPKFDKKGKATAWITKDKSV
                     FFGMVVPSMPAEFTLQPGENLPVIGQTTRQWIVALRRYGDQVSIRLTKNRPDILYKKP
                     VVTAKKEPSRQELLEEELKKYKTAAETVEEPEPPEETGLRSFFGRSSRKDTDKTVKSR
                     RIVIAKGEGDELIAEIDGQKLEPKAEAPPEESPAGEADGSEEAPPVIQAAEEPAPAPE
                     DGTEVPKKKTDMMGLIQWIAIAVLGTGLLTAAGVRIVQKKRANDFKVPDLKLGKGKDK
                     KEIEPEPPAAAAEPDGLSDASVYFKKLANNKVDFSGSLASFSLLDLIQFLNSTKETGD
                     LQIRDDGGEERAEVYFDKGEIIDAVFGKKHGVAAIFSLVKNTEGNFAFFRKKVSEAKR
                     TIEMPTMSLMLEASKNMDEGPAAPPPKPAPKPKPLSLKKKK"
     gene            complement(12524..13081)
                     /gene="folE"
                     /locus_tag="PKK36_00760"
     CDS             complement(12524..13081)
                     /gene="folE"
                     /locus_tag="PKK36_00760"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNR93120.1"
                     /translation="MDKLIREFLVGLGEDPDREGLKRTPERVVDAFKFLTHGYSLDPA
                     EVVKDAIFTVEANHMVIVKDIEIYSLCEHHLLPFTGRCHIGYIPNGRVLGVSKLARLA
                     DVFARRLQLQERLTNQIADAIMTAIEPEGVAVVIEAQHLCMMMRGVEKQNSSMITSAM
                     LGVFNANTTRNEFLQLIKGHLTGNR"
     gene            complement(13081..13761)
                     /locus_tag="PKK36_00765"
     CDS             complement(13081..13761)
                     /locus_tag="PKK36_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNR93121.1"
                     /translation="MQNVIQIAGVIDQAEASMLMDCGVEYLGFPLRLAYHQEDLSEAE
                     AGAIIRALPPPCAGVLITYLDQADEIDRFCRELGARHVQLHGGITVSELKKLRALNPD
                     LCIFKSLVVEQDNTGELRRTVEALAPLADAFITDTFDPATGARGATGKTHDWNISRML
                     VELSPKPVILAGGLNAENVREAILAVRPAGVDSHTGVEGPDGRKDREKVKKFVREARA
                     AFAGIKGS"
     gene            complement(14057..15799)
                     /locus_tag="PKK36_00770"
     CDS             complement(14057..15799)
                     /locus_tag="PKK36_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR93122.1"
                     /translation="MKYLLAALSFLALTAGGGDPVASPHAARGGQITAYAGPWPQSFN
                     YYLNNNVFSATVFSFMYESLLGLDPVTAEYAPGLAEKWEISGDKRTFTFWIDPRAKWS
                     DGKPVTAQDVAWTFSAVMDPKNLTGVHKVSLETIKPPVVVSERCIRFTTDDIHWRNLG
                     AVGGFSILPKHAFATQDFNKINFAFPVISGPYRLSGVRDGVRADFTLREDWWAREKPE
                     NRHVANFKTIAFRFYEEQENAFEAFKKGMIDIFPVYTARLWVNETGDERFLKNWIIKQ
                     KIENYRPIGFQGFAMNMRRPPFDDVRVRRAMAHLLNRPRMNETLMYNQYFLHRSYFED
                     LYSCDIPCPNEFFDFSREKAIALLNEAGWKMNTEKGLLEKNGQPFIFSFLSRDASTEK
                     FLNIYAEDLKAVGIEMKIDRKDWAAWTKDMDEFNFQMTWASWSAGIFKDPEGMWASKE
                     AEREGGNNITGFKNDRVDELIEKQKTEFDIEVRNDICREIDRIAAAEVPYVLLWNINC
                     VRLLYWNKFGTPPTVLSKFGDDSSAFSYWWFDPDSAADLAEAMKNEDHLPPAPPVVNF
                     DRSFRGGSEKDGIQ"
     gene            complement(15816..19127)
                     /locus_tag="PKK36_00775"
     CDS             complement(15816..19127)
                     /locus_tag="PKK36_00775"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNR93123.1"
                     /translation="MNTELFTELYRVMLTSRKLDELAESLAHQGEVPFYVPSCGHEAT
                     AMLAPHLKEYDWLHCHYRDQALALARGVEPLTILYGLLGKTESNSAGRRMPGFPSCRA
                     LRILSSPTVVGNSALQACGVAAAVKAQPEQPVVLCSMGDGCTQEGEVLEALAEAVRSE
                     LPVLFLIQDNSFALSSRTSGRTFYSLPGGEAREYLGRPIAWLDGTRPETVYEVLGRTI
                     ETIRRDRKPQVVVMRTERLTSHTNSDDHRMYRSAEEIAHIRETADPVRILGESLEKAG
                     VELAPIEKQVDETLERQLQQARAGSNPADARDVKKALPPEVSDPAREYRGTGDRKLSM
                     LEAMRTVLRNQLASSPRVSLYGEDIEDPKGDVFGLTRGLSTQFPDQVRNSPLSESTIA
                     GVSIGRALAGEQPVALLQFADFMPLAYNQLFSEMGCMHWRTNGDWEAPVILMGICGGY
                     RPGLGPFHAQTPVSMAAKIPGVDVFVPSTAADAAGLLNAAFASGRPSIFFYPKNLLND
                     RTVATPEDIGEHLVPVGRARVVRTGRDITFVSWGSTMPLCERAVNALEEAGLSAGLID
                     LRTMIPWDVETVMAAAAKTGVLLIVDEDTHTASLSGEIAATVSEMSGGKIRAGRVTTA
                     DAYVPYNFGAQLATLPSFKSVMEKAAALLDWDIAWEQQVKEEGNELIVNAIGSSPSDE
                     TVKITRLHVKKGDPVETGAMIASVEADKATMDIAAPAAGRVSEIFLNEGDTATVGTPL
                     VKIHTAESASARPVTEDNPGRPILNKRAAAPVRAAVTAKAEARPVILSSICSQMGSRV
                     MTNEELVKRCPGWSPDDIVQRTGIMKRHWIGEGESVLTLAVAACRKLLKAENIDILEI
                     DALICSTGTPLSTTPSLACRILKELSPESGELLMQAHDINAACSGYLYALQQAYDMLT
                     HNPAARILVVTAETLSPILNASDPGTYFLFGDAATASLVSCERRKGNINARVSRPALS
                     ALGEAPEILYVPSLNSGEHVMMDGLPVFRMAVKKMIMMLERACAADGITVSDLDMIVP
                     HQANERIIEAIRKAIKFPTEKVYYFIRDYGNTSSNTIPLALESIIPKHRSTNKVGLTA
                     FGGGFTFAAGILEVL"
     gene            complement(19239..20045)
                     /gene="lplA"
                     /locus_tag="PKK36_00780"
     CDS             complement(19239..20045)
                     /gene="lplA"
                     /locus_tag="PKK36_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes both the ATP-dependent activation of
                     exogenously supplied lipoate to lipoyl-AMP and the
                     transfer of the activated lipoyl on the lipoate-dependent
                     enzymes. Creates an amide linkage that joins the free
                     carboxyl group of lipoic acid to the epsilon-amino group
                     of a specific lysine residue in lipoyl domain of
                     apoproteins; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HNR93124.1"
                     /translation="MIIVSDYRDPHLNLAIEEMLMRQAPDKPVLFLWRSLPAVIIGKN
                     QNPWKECDLEFMESMHMTIARRISGGGAVYHDPGNLNCCLITQRTAYNPDDLSAIMLR
                     AIASLGMEARRGGNSLFIQQRKISGSAYCLQKNMALHHCTLLVSADLDALRRALTPHK
                     ECFDSKAVESIPSPVASLKDFLPGITMDQAAETIRRSFGDSRRIHHAADICQHPDFRD
                     IYAKHRSWDWTFGHTPRFSVERGGKSLTVEHGMVTGPAGAGSLIGKRFPG"
     gene            complement(20042..20170)
                     /locus_tag="PKK36_00785"
     CDS             complement(20042..20170)
                     /locus_tag="PKK36_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93125.1"
                     /translation="MEPDFIHNHQMFRDEDHLNLKYGAPEFSRRLRARLEEDGILP"
     gene            complement(20178..21047)
                     /locus_tag="PKK36_00790"
     CDS             complement(20178..21047)
                     /locus_tag="PKK36_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93126.1"
                     /translation="MKALRHILNAAGFTTGLLLVLAGLARFGASRDPIYPAVKTRWDN
                     LFRQRHEIEALAVGHSHCRGIDFDTLGIRGYHVWEGGGDAFETEYIVRHLIPRLPNLK
                     VVFYPASYFTFYFDNAASDDQDRNSNRIKTYAYCPGWTWIRGDWRNFIKGKLINVIRP
                     ERDYLFELLYKNPPAAERVYLREHDGASTAPGLFEPRTLEELISLSETNRAAEHQRLI
                     LDMGKNHRHLAKDARKSVERTLKDLRKHDIQVVFFTPPVFLSLYRTLQSRPCRRTKGR
                     HGSAGRKIRRRLL"
     gene            complement(21047..22531)
                     /locus_tag="PKK36_00795"
     CDS             complement(21047..22531)
                     /locus_tag="PKK36_00795"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNR93127.1"
                     /translation="MLFNSLEFLVFFPLVTAAYFLCPHRFRWALLLAASYYFYMCWKA
                     EYIVLIIVSTLIDYTAGLRMVSAPSGSRRRKAWLMLSLAGNLGLLFSFKYFNFFNDSA
                     RAIFEHFNLMYHVPAFKLLLPVGISFYTFQTLSYSIDVYRGRQQAERHLGIFALYVSF
                     FPQLVAGPIERCSRLLPQFYEKHNFDYVRATSGLKLMLWGFFKKLVVADRLAVFVSTV
                     YQRPCDFEGVSFIMATVFFAFQIYCDFSGYSDIAIGGAQVMGFNLMDNFNRPYFSKSI
                     SEFWGRWHISLSTWFRDYLYIPLGGNRVSKPRWHFNLFLVFLVSGLWHGANWTFVVWG
                     ALHGFYLIFAIWTAGLREKFCALTHLNRLPRLHKTINVAVTFALVCFAWIFFRAGRLY
                     DAFYMVRHLFSGIPNWFGNLIALDTDAVLRPLLVGQTLEDFLLAAGAIGFMEIIHLLQ
                     RHGSMRQMLGTWPLPLRWAACWIMIAAIIILGRFNITEFIYFQF"
     gene            complement(22537..23523)
                     /locus_tag="PKK36_00800"
     CDS             complement(22537..23523)
                     /locus_tag="PKK36_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93128.1"
                     /translation="MKQLQQSILFTAGLIGIVLFCAALWSRMDPVYPDIKFRMQNLLR
                     QANEIEAVAAGNSHCKAIDFDALGERGYHIWLGGNDVFETELHLKTLIPRLPKVRTVY
                     YAVSFHTFVADNRAATILDRKSKRVEAYAYLPGWKWIPGDFQSFVKARLINLIRPDHG
                     YTTLLRLLGQQPRPAKESNPRDGSRNRDRQFKGFRNINVLIERAEKARIVEHAKLMND
                     MLQNCPGLPEDTYACLERTIRFLQAQDIRPVFFTPPYFFRYTELADRNMVETFRRNMK
                     ALARKHNVEYYDFSANPDFVFETKYFMDEDHLNRRHGAPAFSKQLRERMNCR"
     gene            complement(23525..25006)
                     /locus_tag="PKK36_00805"
     CDS             complement(23525..25006)
                     /locus_tag="PKK36_00805"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNR93129.1"
                     /translation="MLFNSLEFLLFFPLVTAAYFLCPHRFRWALLLAASYYFYMCWKA
                     EYGLLLLASTLAGYGGGLCIGTLPQNDSRRHLCLTVSLGVNLAILFAFKYFNFFNDSF
                     RTLFNHFNLFYGVPAFNVLLPVGISFYTFQTMSYLIDVYRGSQRPEAHFGKFALYVSF
                     FPQLVAGPIERAARLLPQFSAVHHFRFDRMCSGLRLMLWGFFKKLVIADRLALFVNAV
                     FDQPENWQGISYIVAGILFGWQIYCDFSGYSDIAIGAARVLGFDLMRNFNRPFISRSI
                     TEFWQRWHISLSTWLRDYLYLPLCGRSTSKRWWVISLLLVFMISGLWHGAAWTFIACG
                     ALHGTYLILEYLSFNTRRRWCEQTGLKKHPRFHRFINIASTLFLLFFGWIFFRAADIR
                     EAVHIIRNLPTGLPAWLNDVFHLRRAALAPVVVGHTLWELLIGVAAVTVMETVQLIHD
                     RQGISEFLAERPVWCRWLIYYSLIMTILVFGRFEVVEFIYFQF"
     gene            25164..26285
                     /locus_tag="PKK36_00810"
     CDS             25164..26285
                     /locus_tag="PKK36_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93130.1"
                     /translation="MKIAFVKQKYVPFGGGELYLERLAGACRDRGDEVHLITAAWPGN
                     RVSAFTVHPVPVRNWSHASVARSFSHNLKEIIRSVGFDVVFSLDRTEQQHIWRAGEGA
                     HPVWLDRRGEFEPAWKVRLARLSSRQRTLLKLERRCVSRTPVIIANSQMVKNDLENCY
                     DLSGKRVEIIHNGVDFSRFSPDGREEARTQVRSRLQVNPDGPLLLFAGSGFRRKGLPE
                     LMRALRGVPDAVLLVIGRDKSAPWIRLAASLGVSGRVRFLAPSESLPELYRAADVTVF
                     PTWFDSFGFVGLESMACGTPLVTTPFAGVSELVREGVNGAVIARPDAADELAAAIQDQ
                     VSRKDRSRAIADSVREYSLENNIRRTLAVIDDTAFSRKS"
     gene            complement(26269..27000)
                     /locus_tag="PKK36_00815"
     CDS             complement(26269..27000)
                     /locus_tag="PKK36_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNR93131.1"
                     /translation="MPDSTCIEPRFTRGEWNVQTDTLQPGDIQLWYFDLSDTNSLLEP
                     ARSRLTPDDAARAGRFLRQEPQNSFMIRRGLLRRLLGLHTGSRLYPCALEYSAGGQPM
                     MPVSRDGLRPLFFSVSHTGSDLLFALAKSWPVGVDIEEERPVRDLQRMMLKSCSGEEI
                     NRISKLPEPERVRAFYKVWTLKEAISKAAGSGLTPEILRNSPSRMFHQYYFTLLNRMA
                     GAVAAEHPVLRFRWRTCPAKLMICG"
     gene            27111..29819
                     /locus_tag="PKK36_00820"
     CDS             27111..29819
                     /locus_tag="PKK36_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR93132.1"
                     /translation="MKVRGEYRSIIDVVEALTATDPGAAACTFLADGLNDRCAWTRRD
                     LYSRACAVARVLSDRKARGQPALLLYYPGLEFLAALFGCLFSGVVAIPAYPPGISARS
                     SRRIESILEDAGSSLILTTSRIRETRRTSMDRHSALPRQAWIETELIALDSAEDCVPV
                     RAQESDVAYLQYTSGSTSTPKGVVVTHGNLFHNLTCIDRTFNVSGGSGIASWMPHFHD
                     FGLVFGLLAPIFTGKPCTLMPPAVFVQNPWLWLKAISDARASHAAAPNFAYDLCNTRI
                     TDAQLETLDLSCITALLNGAEPVRASTLDEFTRRFAVCGFRREMFCPVYGLAEITVGA
                     VCTRFDEPPVCLSVDADAIEQHHAVPAEGNAPKSRLLVSCGHEVFGHRMEIVDPVALV
                     RVPKGGVGEIWLDSPSVCAGYWNRPDETRQTFQACLAGTNEGPFLRTGDLGFMHDGNL
                     YITGRIKDLIIIHGVNYYPQDIELSCEKAHRALKANAGAAFSVERGGSEKLVVVYEQD
                     RTVKDPDIGQIAMAVRHAVSEEHELQIYDFVMVRPGTIPKTSSGKIQRHACKADYLNG
                     SLKVVASSRLEIAAAGERNIAPPADEMERQLARIWEEVLEISPIGVTDNYFDLGGNSL
                     HALRIIGKIGETTGRELQLTDLFQTPTIRGVAELLRRADETAELIALVPLQPAGTCTP
                     FFCVHPGGGMVMCFNDLAHSVGKDQPFYALQSQGLDGRRSPLKRIEDMASLYIREIKT
                     VQPAGPYRLGGMCLGGVIAYEMAQQLVRLGEQVEFLGVLDTRRPPGWYRPLFRQRRVI
                     KEWLGMISHAGQKRVLKRIWLANEKARNRYRPEPYPGKITLFWSDHEGDEQADRQEMW
                     RRLALGGLDIIPIAGASHRAILAAPHVQTLADRIVCCLEPFNGIRS"
     gene            29816..30625
                     /locus_tag="PKK36_00825"
     CDS             29816..30625
                     /locus_tag="PKK36_00825"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNR93133.1"
                     /translation="MSVLAVNVICTSYSGNTWLNLMLGAHSRAFCVGEYKNIRKTGRV
                     NCMLHGADCPVWSRFDASAQEHPYLQLSRLTGKNILVISNSLDYLPADPGPGIEQRFI
                     HLIRDGRAVAASFRRKHRGMNIWQAARRWRHDHRKNLRRLSRHPAEAVLPVRYEELKS
                     DPEREVRRICGFLGIPFEPSMLEYWTRDMHYLGGNQGTLLALARKQGGDLPESSRREQ
                     GREQRDWKLEFYAGSDPQKFTDDRWKSDLTSIQLGIFNLAAGGLNRRLGYQ"
     gene            complement(30626..31831)
                     /locus_tag="PKK36_00830"
     CDS             complement(30626..31831)
                     /locus_tag="PKK36_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNR93134.1"
                     /translation="MKNKSLVFCAAAALSVMLLAGCEFFEPSNAYKIKAGQQTDWDVP
                     PAEVVYDVPPAHTIAVRPAPAVPAYAEEEPVGPADETETLPDPAAIPPPEVFNEEIEP
                     AVTEPSAGGDEALPPDARPGECYARVHIPPRYETVTEQVLLQPESEEIVVIPAQFEWV
                     EEEVLVKEASTELEVVPAEYEWVEEEVLVTPASVRTEEIPARYEWVEEEVMVKPARRE
                     WKKGTGLITTVDNATGEILCLVELPPVYEKVKKRVLAEPASVREIQVPAEYETIRKHV
                     MTKPATTRDVTVPAEYETVRVQKMTAPEREERKIIPAVYQTVSKEVMVEPGRMLWKRV
                     LCESNLSPDIIRQIQTALIEQGFNTGGVDGVIGGQTREAVAQFQKDRDIAEGGLTYET
                     LAALGITVE"
     gene            complement(31906..32718)
                     /locus_tag="PKK36_00835"
     CDS             complement(31906..32718)
                     /locus_tag="PKK36_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR93135.1"
                     /translation="MIEAGKTYLVMGLLDADSLAFAIGKTIERFGGRVIYTVQNEVFK
                     KRFLDRAEALSRQEIEALDLRFCDVTVDEEVQELFTDLGPLSGLVHSIAYANPNTCLG
                     EEFHTSEIDDILLSHHISCVSLATVLRHAQPSLKDGGSVVALSFDTAHVYPYYNWMGV
                     QKAALEALVRALARRHGRDLIRVNAVSAGPLFTKAASKIPGFGSLTSLWETVSPLPWD
                     TLKDKQAVADAVTFLLGPYAQKITGQILTVDGGAAITGGALMGFEKRAHPPD"
     gene            32925..33878
                     /locus_tag="PKK36_00840"
     CDS             32925..33878
                     /locus_tag="PKK36_00840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNR93136.1"
                     /translation="MTPAPAERKWTECGPGPRAVLYFLAAITAGLLIAPPVFNLVTWL
                     GREFQWLAGLRETAFEKLLNRCVILCVMLALYPAIRVTGLNSWKALGLSRDDRGLRLF
                     WIGWIIGTVSMVGLMLTGWASGAMYYLPGRVDWSLAGKVVSYMFGAVVVGVVEEILFR
                     GAALGMLRRGLRLLPAALLLSFVFALAHFATPEPAVGVVRGHWDSGLRMLPYLISVED
                     MRWEYAFMFITVFFMSMTLCFLYAAYGNLYLAIGLHAGWVWLMRIGGHLLERDDNVMM
                     VLFGPSMSVAKSGMAIIEVLLFFAAALFLYGRKRRTVPDPA"
     gene            33875..34585
                     /locus_tag="PKK36_00845"
     CDS             33875..34585
                     /locus_tag="PKK36_00845"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNR93137.1"
                     /translation="MSLNHILDMVYPRACIGCHARAPENSPYLCWDCQSSIMFVQPPF
                     CSQCGDPVSGYIDHDYICSFCAETRPAFDRARSVARYEGVVRQMLRDLKYNDAIWLVG
                     DLARMLEICARAHYDLAAVDVVCAVPLYGARQRQRGYNQSRVLAEALAERIHKPFISK
                     GFRRIRPTPTQTDLTAGERLANVKDAFSAVSRGWIEGKRILMIDDVMTTGATVHEASR
                     ALRKCGASRIYVLTAARG"
     gene            34598..35464
                     /gene="accD"
                     /locus_tag="PKK36_00850"
     CDS             34598..35464
                     /gene="accD"
                     /locus_tag="PKK36_00850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNR93138.1"
                     /translation="MFPFFRKKSSTLTARKRDVPDGLWTKCPSCSELVYRKQMHRDHG
                     ICAKCGFHFPIGAAERIEMLLENKSFEEWDAGLESVDSLEFTGTSSYVSKLESNMKKT
                     GRKDAVICGRGRIGEHAVALAVMDFDFLAASMGAVVGEKITRLIERATAERLPVVIVC
                     ASGGARMYEGLFSLMQMAKTSGALARHAAAGLPYIPVLTHPTTAGVMASFATLGDVIV
                     AEPDALIGFAGPRVIKETTQQDLPDGFQKSEFLLKHGLLDMVVNRKELKSTLIQLLDY
                     FRPHRQGEAKAS"
     gene            35461..36777
                     /locus_tag="PKK36_00855"
     CDS             35461..36777
                     /locus_tag="PKK36_00855"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNR93139.1"
                     /translation="MSSTLASSLEKLFRRTAFGIRPGLEVTEAILSELGDPHRRTPCV
                     HVAGTNGKGSVAAMMESVLRAAGIRTAFYTSPHLVDFNERFRLCGQPVGDDELARLLE
                     DVEQAADAASRKHGLRPATFFEISTALAFEYFARAGAELAVIETGMGGTWDSTNVVIP
                     LVSVITRIGMDHMEYLGHTLEAIAAEKSGIIKPGVPVVFGAMPEEASAVLLRRAKEAG
                     ASALAAAGYASVRRMKQGLDGQRLKIETLQTAYPPVLLPLLGDHQLENTATAVAALET
                     LAGRLETELAADAVKHGLEKTFWPARCQILSREPPVMLDGAHNESGAAALAQTLKGLR
                     EKRPLRMIVSFLADKDMDGFLRHFGRQADHAWAVQLHCDRARPVEDIMAALQRHHIPC
                     ESRLLPDALAEARTWAVENRGMVCIAGSLYLAGEVLKLEQGRLNFI"
     gene            36817..37923
                     /gene="murG"
                     /locus_tag="PKK36_00860"
     CDS             36817..37923
                     /gene="murG"
                     /locus_tag="PKK36_00860"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNR93140.1"
                     /translation="MKKLKIALACGGTGGHIFPGMATACVLRARGHEVTLWVAGKDVE
                     STALAGWDGPVLTVKARGFPHGVSLKALGSAWQLLCAVRKCRAMMKKDRPDVVLAMGS
                     YASVGPVGAARRLRIPVVLHESNVLPGRTNALFARGASAVAGCFEETRFYMKRKDIVL
                     TGMPLRRELEAASAVLPSLPPEGGPRTVLIMGGSRGARRLNEVASRAVCELHRKGSAV
                     EVIHLAGVADEKEVRSIYEEAGVPHKVESFIQNMAPVYQKTHLAICRSGAATCAELSA
                     FGIPALLVPYPFASKDHQTANARAVEKKKAADVVDEKDMTCEFLVDYLTRIFSSPDRL
                     AAMSQAARKRYSGRGAEALADLVEQTAAGGRETN"
     gene            37923..>39050
                     /gene="murC"
                     /locus_tag="PKK36_00865"
     CDS             37923..>39050
                     /gene="murC"
                     /locus_tag="PKK36_00865"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNR93141.1"
                     /translation="MPDRDVASIRLWLRSGQGHAHFAGIGGVGMAGLAFLVGKAGFRV
                     SGCDLVESRITAWLRANGIQVHSGHDAAHIDQSVTALIRTPAVRPDAPEVKRAREKGV
                     PVYLRGAVLAALIEDRASIAVTGTHGKTTTSTFTAQLLSACGRDPSFCIGAEVAGPGV
                     VAHAGRGKQIVVEADESDGTVALYRPETALLTNIEYDHMEHFGSEAEMEDCFRAFARA
                     ARRHVIWCVDDPGLRRVMAGIGNGITYGLSENADCRAAVEKMTADETTIRLWWRGADQ
                     GLFTCAAAGMHNAVNAAGAAAAAHLYGCSFDEIRDGFCELHLARRRFEQVCDLKGVRV
                     ISDYAHHPTEIRALIEVARLQPHRRIIAVFQPHRYTRTRALG"
BASE COUNT         7662 a        11482 c        11903 g         7993 t
ORIGIN      
        1 tggtttaatc tcccgctatg gggccggcaa tacgcggcgg ttgtgaattt ccgctttgca
       61 cagacgctgt ctctccttct tgatggcggg gtatccctgg ttgaggcttt cccgcttgcc
      121 ggacgggcga cgggcagccc ctgggtgacc gatctggtgg atcgggaagg cgagtcgctg
      181 tcgaggcacg ggagcagcct ggcggatgcc gtgcgcagga ttgcgccgct caacgatgtg
      241 ctcccaggct ggattcaggc cggcgaggcc agcggggctc tggcggaact gctttcccgg
      301 gccggcaggc gctatcagct gcagtggcag aggatgatcg gccgttcact ggaagtgctg
      361 gaacccgctc tgattattct ggtgggcggc ctggtgctgc ttctggcgct ggctgttctg
      421 actcccattc tgtcgctgaa cagttccctg atgtgatttc atagagaaca ccccacagct
      481 tgcccgttac aaaaagccgt ttcccgtccg catcccatgc gatgccattc agcacgtctt
      541 ccgtacgcat gggcgatctg ggacgcaggg cctcgcagtt aatccaccgg acaaccgaac
      601 cgctctgcgg atcaatgcag gcgacgcgat cggacttcca gacattcgca tagatcagcc
      661 cgttgacata ttccatttca ttcagcaggg agagtggttg tcccctgtct gtgacctcga
      721 tccgtccgga cggctcgaac gtctctgcat ccagaaacgt cagcgtggaa gacccgtcac
      781 tcatgatcag ccgtgatccg tcacagcaaa ggccccagcc ttcccccttg taacggaagg
      841 tcctgagcat cgtcagcgtt tctgcatcat aaacatatcc cgtttcagca tgccatgtga
      901 tctggatcag gcgccctttg cacagcgcga gtccctcggc aaacacgctg tgagggagca
      961 tgattttccg gacgatttct cctgtttccg gcacgatttg gcggagcgag gaccgtccat
     1021 atattcccgt gctttcatac aggaagccat tgtaatagac gagcccctgt gtgaaagcgc
     1081 gcggatcgtg cgggaatgta tggacgaccg cagccatgac ttcgggcgtc tccgccgacg
     1141 ggacgtgcac ggatgccggt cggcatccaa gcagaagcag gacggccgcc gctgcctgaa
     1201 atcctcgcgt catggctgat caggccagca gttggcgggc tgtctgggcg atatgctccg
     1261 ccgtgaaacc gaatttttcc gcgagcacct cggccggagc agaagccccg aagcgatcaa
     1321 gacagaccat ggccccggcg cttcccgcat acttctgcca tccatgtgaa accccggctt
     1381 caaccacgag gcgttttgcg caggcggcgg gcaggatttt ctccttcaga tcgttcggct
     1441 gcgaatcgaa gagctcccat gaggggaaac tgaccacgcg gatggcggct gattccttct
     1501 ccatggactg tgcggccttg agtgcgggag ccacttccga gccagtggcc agaatgatga
     1561 ggtccggtgc gccgtcccgg ttttgccaga gcgtgtaggc gccctgctga agacccgccg
     1621 ccggggcgca gacggatcga tccagcgttg ggaggctttg acgcgagaga atgagcgccg
     1681 tcggtccgtg ccggttgcgc agggccgcgg cccatgcctg cgcagtttcg gcggcatcgc
     1741 ccggccggat caccgtaagg ttgggaatgg cccgcagtgc agccaggtgc tccaccgcct
     1801 gatgtgtggg gccgtcttct ccaaggaaaa cgctgtcatg tgtgaacaca taaatcaccg
     1861 ggagatgcat aagggcgctt aaccgcacgg ccggccggca gtaatcggaa aacaccagga
     1921 aggtggcgcc ataaacaatg aaccctccat gcagcgccat cccgttcatg atcgcggcca
     1981 tgccatgttc gcggatgccg aaatgcaggt tccgcccggt catgcagccg gcctgaaccg
     2041 aatcaaaccc cttgagttcc gtattggtgc tcggagcgag atcggccgac ccgcccacga
     2101 ggctgggaat cgctttcgcc agttcctgca aaacggcgcc ggacgcctgc cgcgatgcca
     2161 tcgccttgtc cggagaaaat ttcggcagga ttttgtccag attcgcagga atgctccctt
     2221 cttgagccgc atcccattgc gccgccagcg caggatcggc attgcggaat tccgcgcagc
     2281 gcttgtgcca ctgcaaatag tcattggaca actgttgttt ccgctcggaa aaaacggacc
     2341 gaacctcgtc cgggacatgg aagaacttgt cctctggaag cccgaggagc ttctttgtcg
     2401 cggcgatctc ggcgtcgccc agcggagacc cgtggctgcc ggatgtgtcc tgcttgttgg
     2461 gactgccttt ggcgatgtga gtccgcgcga tgataatggt cggccgatcc ttctctttct
     2521 ttgcggcagt cagcgcctga tcaatttcct catgattatg gccgtcaatg ctcagcacat
     2581 tccagtgata tccctcgaaa cgccggcgga catcatcgct gtacgccaga tcggttttcc
     2641 cctcgatggt gatgcggttg tcatcataaa caagaatgag cttgttcagg cggagatgcc
     2701 cggccatgga gaaaacctcg tgtgtgatgc cctccatcat gcatccgtcg cccgcgaaaa
     2761 cataagtgta gtggtccact atctccgatc caaatcgggc ggccagcatg gcttcggcca
     2821 gcgccatgcc gacggcgttg ccgccgccct gtcccagcgg gcctgtggtg gtttccaccc
     2881 cgggggtgag atcgttttcc ggatgcccgg gggtgatgct gtcccattga cgaaatgcct
     2941 tcaggtcatc catgctgacc gaatagccgg ccagatgcag caggctgtac agaagagagg
     3001 aggcgtgccc gttggaaagc acaaaccggt cgcggtccgg ccagcgaggg tcctgcggat
     3061 tgtatttcag atgtttgaga aaaagtacgc tggcaatgtc cgccatgccc atgggcgccc
     3121 cgggatgacc ggaattggct ttctggacga tatccatggc caggccgcga atggtgtccg
     3181 ctgtcagttg tgtgttcatg atatgctcct gatgtctatt tttgtgtgca gactgtagcg
     3241 aaaaccggag gccggttcaa tgacattaag cagatactga tgatatgcga gaaaatgact
     3301 ggcgcgcgtc ccgttcagag ggtatcttgc cctgagtcaa attttcccgg aaggattcag
     3361 atgatagatt tacatatgca ttcctcgttc tccgatggca gcttcacgcc cgaggaactg
     3421 gtgaacaaag cggttgaact gaatgtttcc gcgatctcgc tgacggatca tgacaccatg
     3481 gcgggagtgc cccgttttct ggaggcgggc gcgcggcaga acctgcaggt gatcgcaggg
     3541 gtggagatca gcaccgatta caagggccgc ggcatgcata tcctgggcta cggcatgaat
     3601 atcgcaaacc cggaacttca gcagagcctg gactggatcc gcgccggtcg ggaagcgcgc
     3661 aacatggaaa ttctggagaa gctcaaccgg ctgggtttca ttatgacctg ggaagaggtc
     3721 gtttcccgcg cgggggagga tgtcgtcggc cgtccgcatt ttgcccagac gctcatcgac
     3781 aagggctatg tgaaagacaa atatgaggcg tttgacaagt ggctgggcca tgggaagccc
     3841 ggttatgccg accgccggcg actggatccg gaggccggca ttcgcattat cagaaaggcc
     3901 ggaggggtgg cggttcttgc tcatcctttt acactgaacc tcagcaatac cggcctgcac
     3961 tcccttcttc tggaattgag ccgtgccggg ctggagggca tcgaggcgta ttacgcggag
     4021 cacaagccgg acatgcagga cctttattta aaaatggccg cggaatacga tctgctggtg
     4081 accggcggaa cggatttcca cgggtccctg actcccatgc tccggatcgg tttcggcttt
     4141 ggatcgctca atgtcccgga cagtcttctg gagcccctgc tggcccggat cagcagccgt
     4201 gccggctgac ggcggttacc agcccgctga aggcggccgg ctgaggatca catgccccag
     4261 catggctttt ctcaactccg aatgatttcg aagattgggc ttctcgcgcg gtgcgtttct
     4321 gctttgcggc gagaaaaagc caatacttaa attgggcatt cttgcactcg gaatatccgt
     4381 gatcagatcc cgaacggatg tgttcagcag ggacatctgc cggccgatct gcacctccgc
     4441 aacagcggca ggctgcgctg ccggcggctt gtgcacagtc ggagactccc ggcgtattcg
     4501 ccgggatgac ggggccgagc gccgttcggt cgggatctgc accgtcgcac gaggtctggt
     4561 ttcacgccct ccggtcaggc tgtcaaggaa ggcctgaagc tcctgcggtg gaagtttggg
     4621 ctttgaaacc cgcggggcgg ggggaggggc cttcttattc gccttccggc tggccgcggt
     4681 ggctttgctg acgatccaga tgatgatcat gatcgtccag aagatccctt ctccactttc
     4741 ggctctgaga aactgattca tggcatgaat ccccgacccc ggctgcggca cgggccgcag
     4801 ccgggagtca tctgcgggtt atgactgttt gggcggattg ttcccggagt ctccctgtcc
     4861 gaggctttcc ctcatggagg tgtccgnnnn nnnnnncggt agaagtccat gacccccaga
     4921 ttgccctttt caaaagcttt ggcaatggca agcggcacct gcgcctgcgc ttcaatgacc
     4981 ttggcctgca tttcctgaac acgcgcgcgc tgttcctgtt cggaggcaat ggccatggcg
     5041 cgacggcctt ccgcctctgc ctgacggagt ttcttgtccg cctcggcgcg ttcagtctcg
     5101 agcagcgcgc ccacgttggc cacctccctt gcgccgctca ccccggccac gctcacatcg
     5161 gcgatatcga tcgaaacgat ttcaaatgcg gtttgcgcat ccagtccgct gttcagcact
     5221 ttgcggctga tatgatccgg attctccagc acgtccttgt aggtggtgga ggatccaatg
     5281 gcgctcacaa tgccctggcc gacgcgggca atgatggtgt cctccgttgc gccgccgacc
     5341 aggcggtcga gattggcccg gacggtgacg cgggccttga ccagcagacg gatgccgtct
     5401 ttggccaccg catccagcat ggttgcgcct ttggccggat ctggacagtc aatcaccttc
     5461 gggttcacgc tcgtacgcac cgcgtcgtac acatcgcggc cggccaaatc aatggcggcc
     5521 gcgcgctgga aggcgagctc gatgttggct ttgtccgccg cgatcagcga ctgaaccacc
     5581 gggatcacat tgccgccggc aagatagtgg gcttcaagct ggtcgctggt gagcggcagg
     5641 cctgccttga cggcacgaat gcgggcatcg acaataaggg tggggggaac cccgcgcagc
     5701 ttcatgccga caagattgaa gatgctgacc cgcgcgccgg acatcagggc gcgtatccat
     5761 accttcagaa aatagatgaa cgtgaagatg atgatcaggg cgacgaccac cagtacggct
     5821 gcgataatag gcattttcag ttctccttat tctgttttgc tgctgtcagt attcgtttgc
     5881 gaaatctctt gcgggggctt tgcacctggc ggctgcacat aaatgatcga acccgccgcc
     5941 acgttgagga ccagggcccc caatcccgcg cacacggcca caatccagat accgccgctt
     6001 attttaggaa ccagcgtcag gatgtaaagg caaagcaggc cggcggtgat gatcagcagc
     6061 ggggatgcgg gcgtctttcc tccgcgcgaa cgaaggacga tgcgtccgag cagaacagcc
     6121 gtcacaatcc gcgatacata cagcaggatg gcaaagatgc cggtccccag cagggcaaga
     6181 ggaatgccga tcaggctgat cgccagcaga agaataacgg gcagcatgag aaaacagacg
     6241 gccagaccgg tgaaaaaaca ccgccaggga gccacggaaa gctgatgcgc ggcccggctg
     6301 agcgccagcg gggcgagtct cagccatacc caggccacca gcaaagcccc catgaactgg
     6361 accacgcgaa gctgcagacg cagcgcccag ggcatcgcgg aggatgatga ggcgggctgt
     6421 gtcaggggtt ttcgaaccag caggccctca tgcttgacgc gggggtccag aaacagttcc
     6481 ttgctggaac tgtatacaat atctccgcga agcaccgcgg aaggcatgac ggcaatgtcg
     6541 cttccaataa tccgaagggg gccttcgatt gtcccggcca gcgttatttc atcgccaatc
     6601 agttgtgccg cgccgccgat ttttccctcg accacgattc gtttggatgc cagcaccgca
     6661 tctccgtcca caactccttc cggagtgata tagatcgagg ttccggaagc gctcatcgcc
     6721 gagcgcatga tccggccccg cacggtcacc tgctgtgccg ccagcagccg cgcatgcccc
     6781 tgcgcggtgc cgctgaagtc agccgtttcc gcggcgcacc agacactgcc gctgaatgtt
     6841 ccgcccagca gtgccgtctg cgccagagca tgaaaatcgt ctgaaacggc gccccggact
     6901 tccgtcgtta cggccgccag ccagagttgt ccctcctgca gttcgtttcc cgccagccgg
     6961 tattcattcg tgctgcggaa atcaatggcc gcccccgcct gaagggctgt cagcagtacg
     7021 gccgccggga tcagacgcag aagcctcatc ccggttctcc cttccgcctc acggtgacat
     7081 gatttccctt cgcggagatc acccggaccg gtgtcccggc cgcaatccat tccccctccg
     7141 cgacaacatc catgcgcctg ccgttgatca cggcgattcc ggccgggcgc agatcggtct
     7201 gggcgctgcc ttcggcgccg acaagcggcg gattggcctc cgaatcaagc ttgaaggact
     7261 gcccgtcctt tgagagcgtc agcgcgcgac cgatgggggt attgggaaat attctgatcc
     7321 agagcacgag cccgaccgcc atcaggatga gcatgagaat agcgacaaga aaaccggtct
     7381 gcgggccgaa gacggcaaac gcggaaatca ccgcgccgaa cagcgcgacc gcgccgagag
     7441 tgcccagcac gccgccggga acaaaaattt cgacggccag cagcagaaaa ccgcagaaca
     7501 gcagcgtcag gaaaagcatc ataaccggaa ctccttcatg attccaaagg ctccacaatc
     7561 acctgcaggc cgtgagtctc cacaatgcgc acggccgatc ccgcctcaat atagttcccg
     7621 cggctcacga tatccagttt cctttcgcca aacatgccgg tcccgcccgg ccgcagatcc
     7681 gtcagcgcgc ggccctgcag ccccaccagc tcttcggggc gattggatga gctgacgaac
     7741 cctttgtcgt gcgaggtgtc ctcggccagc accagccggc tggtggcggg atgccttgaa
     7801 atccagcgcc ccagaagcat cgcgatcacg acggagccga tcagagaaaa tgtcagcttc
     7861 agaaagggta tgtagagcaa attggggtcg ggataccagg ggccgcccgg ataatgctgg
     7921 accatggcgt tcagcagggc ccacccgatg cagagcagcc cgagcaggcc ggccgcccca
     7981 aaaccgggga acacaaatat ttcgagcagc agaagcagca cgccggccag aaagatcagc
     8041 aggtcctcca tcccggccag tcccgcaata tgatggcccc agaaaaaaat ggccagcgcg
     8101 aggacgcccg taatcccggg caggccgaac ccgggtgttt tcacttcaat gtaaatcccc
     8161 agcatcccga tgatgaggaa gaccggtccc agcgcggcga tgaagcgcgc aatgcgctca
     8221 gcggtggtga tgcccagctc gcggacctcg gcattgtcca tgccgatcaa cttaagcaga
     8281 ttttccagag agtccacggt cccggcggaa agaagcggcc gctgatcctc tccgtatttc
     8341 ttttcggcct ccgcgctggt cagcgtgagc agctgccctt cgggcgatat gacctcgtcg
     8401 ccgattttat attcaagttc ccggcggacc atggcttcgg ccagtttgtc gttgtgcccc
     8461 ttgtgctggg cggtggcgcg aatcaggccg gcaacgtagg acagcatctt ctcttccgtg
     8521 ccatccggca ggctctggac cccgccgaag ggggacatca tgatcggcat ggcgtcgccg
     8581 attttacttc ccggcgtcat ataaatgtga tcggtggcca tggcgatgat ggcgccggcg
     8641 gatatggcgt tcctgtcgac cagtgtgtag gtcgggacgg aaacatgctc cagaatgcgt
     8701 atgatttcct ccgcggcctg aatcgtgccg cccggagtgt ccatctgaag gatgatcgcg
     8761 cgcgcttttc tctgctcggc ttcggccacg ccgcggcgga tgacatacag cagggcgggt
     8821 tcaatcatgc cttcgacggg aatgacatag accgggccgg gcgggctttc caccgcggcg
     8881 ggaagcggga gcggcgcgga tgccgcgcaa atcaggaaca gaatgcatat ctttttcata
     8941 agcggcctca acccgtgatt attcatttgc tgatgcgaat tatagaggat atgatggctc
     9001 cgcaacagtt attaatcatg aatctcaaga atcgcagaat actggtgacg ggcggggccg
     9061 tgcgcgtggg acacggcatt gccgccgcac tggccgcggc cggcgcggtt cccgtgatcc
     9121 agtatcacca gtccggcgcg gcggcggatg aaaccgtcgc ggaactggcc gggcagggat
     9181 actccgctca ggccatcaag gcggatcttt cctatgccga cgagtgcgag cggctcatca
     9241 gtgacgccgg acctctggac ggactggtca acaacgccgc catttatctg ccggacaccc
     9301 tgcgcgagtt cgatcccgaa gaatcacacc ggcagctgca gatcaacgcc atcgcgccga
     9361 tcctgctgat gaagctcttc gcgcagcagt gcaaatccgg cgccattgtg aatgtgctcg
     9421 accgcaacat ccgccggccc gatccgcgct atttttctta ttcactgagc aagaaaatgc
     9481 tctatgaggc caccctccag gccgcctgtg atctcgctcc cggcatcacg gtcaatgcgg
     9541 tcgcccccgg gcccgtgctt ccccccttgc atgtgaaaga gccggccgga gccctgatgc
     9601 tgaagcaccg gccgacggtt gaggatgtcg gagcagccgt ggtgtatctg atgtccgcgc
     9661 ctgcagtgac cggacagacc ctttttgttg attcgggaca gcacctcggg ctgacggccg
     9721 tggatgcgtc ctgatgtcct tcagaacgga aagacaggcg aacgatgtca tacgatctga
     9781 ccagtctgct ggtggtggcc atctcatcca ctgcgctgtt tgaaaaccgc gccgagcatg
     9841 atttgtatct caataaaacc ctcgacgatt acattgacca tcagatcagc cgcgaaaatg
     9901 agcccttcgc ggcgggcgcc gcgctgccgc ttattcgcgc gctgctgcgg ctcaacgaac
     9961 tgacgcccgg ccgccggctg gtggaagtgg tgattctttc caacatggag ccggaagtcg
    10021 gcgtgcgggt gatgaattcc gttgagcatc acaagctgga tattacacgc gcggccttca
    10081 taggcggtga gcccgtggcc aagtatctgg ccgcctacaa cgtgaagctt tttctttcgc
    10141 gcagtgaaac ggacgtgcag gaggcgctgg actgcggcgt tgcggccggc ctgctctatg
    10201 atccgcccgc gcgcagggaa gaggacgaca tgaagcagct ccggatcgca tttgacggtg
    10261 atgcggtgat tttttccgat gactccgaac ggatttatca ggagcagggg attgatgcct
    10321 tctatcgcca tgaaaaagcc aatgccgaaa accccatgtc ggccggcccc tttgcggcct
    10381 tcctgcatgc catcgcggag atacagaagg agtgccgcag gcagggcggc gaggctccga
    10441 tccgcaccgc gctggtgacc gcccgcagca gccccgcgca caagcgcgtg attctgacgc
    10501 tgcgcgcgtg gggcatccgc attgatgaga tgtttttcat gggcggcgtg tcaaaagacc
    10561 agatactcaa gcagttcaat ccgcacatct tttttgatga ccagcacgcc cacgccggcc
    10621 cggcgtccga gtttgtgccg tcggcccgtg tgctgacgcc gcgccgcccg aaaaaccccg
    10681 caggagggac ccatggataa aatcatcgtc agggagattg cggtgcagtg catcatcgga
    10741 gtgcatccgg aggagcgcgc gcgctatcag cggctgctga tcagcctgga gctggaggcc
    10801 gacctcatgc cggggatcga gaatgacaac attctcgaca cgctcaacta ccaggccgtc
    10861 tgcgaggaag tggcttcctt tgtcacagcc agccgctgct ggctgattga atccctcgcc
    10921 gcgcagatcg ccgcgaagtg cctggaattt ccgcgtgtgc ttgccgtgac tgtgacggtg
    10981 gacaagccgg aggcgctgcc gggaacttca ggcgtgtccg tgtgcattca ccgccgccgg
    11041 ccctgagtta ttttttcttc ttcagcgaaa gcggctttgg tttcggcgcg ggcttcgggg
    11101 gaggagcggc cggcccctca tccatgttct tcgaggcttc cagcatcagg gacatggtgg
    11161 gcatttctat cgtgcgtttg gcctcgctca ctttcttccg gaagaatgcg aaattcccct
    11221 ccgtgttctt cacaaggctg aaaatggcgg cgaccccgtg ctttttcccg aacacggcat
    11281 caatgatttc gcccttgtcg aaatagacct cggccctttc ttccccgccg tcatccctga
    11341 tctgcaggtc gccggtttct ttggtcgagt tcagaaactg gatcaggtcg agcagcgaga
    11401 aggaagccag tgaacccgaa aagtccactt tgttgttcgc cagcttcttg aagtagacac
    11461 tggcgtcgga cagcccgtcc ggctctgccg cagcggccgg cggttcgggc tctatctctt
    11521 ttttgtcttt gccctttccg agcttcaaat cgggaacctt gaaatcattc gcccgcttct
    11581 tctgcacaat ccggactcct gccgccgtca gcaggcctgt tcccagaacg gcgatggcga
    11641 tccactgaat cagccccatc atgtccgttt tcttcttcgg gacttccgtc ccgtcttccg
    11701 gagcaggcgc cggttcttcc gcggcctgaa tcaccggagg ggcttcttcg gagccgtccg
    11761 cttccccggc cggcgattct tccggcggtg cttcggcctt cggctcaagc ttctgtccgt
    11821 caatttccgc aatcagttcg tcgccttccc ccttggcaat gacaatcctc cgggatttaa
    11881 cagttttatc cgtgtccttc cttgagctcc ttccaaaaaa ggatcgcagg ccggtttctt
    11941 cgggcggctc aggctcttcc acggtttctg ctgcggtctt gtatttcttc agttcctctt
    12001 ccagcagctc ctgccgggag ggttccttct tcgcggtcac cacgggcttc ttgtacagga
    12061 tgtcgggccg gtttttggtc agccggatac tgacctgatc cccgtagcgc cgcagcgcca
    12121 caatccactg ccgcgtggtc tggccgatta ccggcagatt ctccccgggc tgcagtgtga
    12181 actcggccgg catcgagggc acaaccatgc cgaagaacac cgacttgtcc ttcgtgatcc
    12241 atgcggtggc cttgcccttc ttgtcaaact tcgggaaggc ccccagcgcg taactcccgt
    12301 ccggctgctc caccttgacg atataggcgt tggtgagctc ctgatagagc ctcaggggtt
    12361 ttccaatctg cgcggcggtt tgaagatcat acacggggaa agtgacccgg gttccgatca
    12421 cctcaatatg cggcgcggcc gcgtcatcct gcgcccccgc cgtcatcacg catgccggaa
    12481 ggacaaccag catcgtgatt atgattcccc gattcatccc catttagcga ttccccgtca
    12541 aatgcccctt gatcagctgt agaaactcgt tgcgcgtggt attggcattg aaaaccccga
    12601 gcatggcgga agtgatcatg ctggagttct gcttctcgac accgcgcatc atcatgcaga
    12661 ggtgctgggc ttcaatcacc acggcgaccc cttcgggctc aatggctgtc atgatggcgt
    12721 cggcaatctg gttggtcagc cgctcctgga gctggagccg gcgcgcgaag acatccgcca
    12781 gccgcgccag cttgctgacg cccagcaccc ggccgttcgg aatatatccg atgtggcagc
    12841 gccccgtgaa gggcagcagg tgatgctcgc agaggctgta tatctcaatg tccttcacaa
    12901 tcaccatgtg attggcttcc accgtgaaaa tggcgtcctt cacgacctcg gccgggtcga
    12961 gggaatagcc gtgggtcagg aacttgaatg cgtccaccac gcgctcgggg gtgcgcttca
    13021 gtccctcgcg atcggggtct tcgcccagcc ccaccagaaa ttctcgtatc agcttgtcca
    13081 tcagcttccc tttatgcctg caaatgccgc gcgggcttcc cgcacaaact tcttcacctt
    13141 ctcccggtcc ttgcgcccgt ctggaccctc gaccccggta tgactgtcca cccccgccgg
    13201 ccgcaccgcc agaatggcct cgcggacatt ttcggcgttc agcccgcccg cgagaatcac
    13261 cggtttgggc gacagctcca ccagcatccg gctgatgttc cagtcatgcg tcttccccgt
    13321 tgctccgcgg gccccggtcg cgggatcaaa cgtgtctgta atgaacgcgt ccgccagcgg
    13381 agccagcgct tccaccgttc ttctcagttc gccggtgttg tcctgttcga ccaccagact
    13441 cttgaatata cacaaatccg gattcaatgc gcgcagtttt ttcagttcgg acaccgtgat
    13501 cccgccatga agctgcacat gccgcgcgcc cagctcgcgg cagaagcggt caatctcatc
    13561 cgcctgatcc agatacgtga tcagcacgcc tgcgcagggc gggggaaggg cgcggatgat
    13621 cgctccggcc tcggcttcgg aaaggtcctc ctggtgatag gcgaggcgca gcggaaaccc
    13681 cagatattcc accccgcagt ccatcagcat ggacgcctcg gcctgatcaa tcactccggc
    13741 aatctgaatc acattctgca tattgggctc gtttataccc gtttgacgca aaacttgcat
    13801 gcaaaaaaga gaaacgggct ggaagcgtca ccctgaggga aaaacgcaaa aaaagccaaa
    13861 aaatggccaa aaacggacca aaaacggcca aaaaacacga aaaaacctcc aaaaacggcc
    13921 caaaatgctc aaaaaacgcc caaaatagcc caaaaatcga aatagtacta aattagtact
    13981 attttaacgc cggcgcccgg ttttgttccc gttgaacctt atgaagatcg ggaagaccgc
    14041 cgatgctgaa ttctgactac tgaattccgt ctttttcact tcctccgcgg aagctccggt
    14101 caaagttgac gaccggcggc gcgggcggga gatggtcttc gtttttcatc gcctcggcga
    14161 gatcggcggc tgaatcgggg tcgaaccacc agtagctgaa cgcggagctg tcgtcgccga
    14221 atttggaaag cacggtgggc ggggtgccga atttgttcca gtagaggagc ctgacgcagt
    14281 tgatgttcca gagcaggaca tacggcacct ctgcggcggc gatgcggtca atttcgcggc
    14341 agatatcgtt ccgcacctcg atatcgaatt ccgttttctg tttctcgatc agttcatcca
    14401 cgcggtcgtt tttgaagccc gtgatgttgt tgcctccctc gcgctcggct tcctttgagg
    14461 cccacatccc ctccgggtcc ttgaaaatcc ccgcgctcca tgaggcccat gtcatctgga
    14521 agttgaattc gtccatgtcc ttggtccacg cggcccagtc cttcctgtca attttcattt
    14581 ctatgccgac cgccttgagg tcttcggcat agatgttgag aaacttttcc gtggaggcgt
    14641 cgcgcgagag gaatgaaaat atgaaaggct gaccgttctt ttccagcagt cctttttccg
    14701 tgttcatttt ccatccggcc tcgttcagca gggcgatggc tttttcgcgg ctgaagtcga
    14761 agaattcgtt ggggcagggg atgtcgcacg aatagaggtc ctcgaagtag gaccggtgca
    14821 ggaagtactg gttgtacatc agggtttcgt tcatgcgggg acggttgagc aggtgggcca
    14881 tcgccctgcg gacccgcaca tcatcgaacg gcggcctccg catgttcatg gcaaagccct
    14941 ggaatccgat cggcctgtag ttttcgattt tctgcttgat gatccagttt ttcaggaacc
    15001 gttcatcgcc ggtttcattg acccacagcc gcgcggtgta gacgggaaag atgtcaatca
    15061 tgcccttctt gaaggcctcg aaggcgtttt cctgctcctc gtagaaccgg aaggcgatgg
    15121 ttttgaaatt ggcgacatgc cggttttcgg gtttttcccg cgcccaccag tcctcgcgca
    15181 gggtgaaatc tgcgcggacc ccgtcacgga cgccgctcag ccggtagggg ccggatatca
    15241 cggggaaggc gaagttgatc ttgttgaaat cctgtgtggc gaaggcgtgt ttcggcagga
    15301 tgctgaaacc gccgaccgcg ccgaggttgc gccagtgaat atcgtcggtg gtgaagcgga
    15361 tgcaccgctc gctgaccacc acgggcggct taatggtttc gaggctgacc ttgtggactc
    15421 cggtcaggtt tttggggtcc atgacggccg aaaaggtcca tgcgacatcc tgcgcggtga
    15481 cgggttttcc gtcgctccat tttgccctgg gatcaatcca gaaggtgaag gttctcttgt
    15541 cgccggagat ttcccatttc tcggccaggc cgggggcgta ttccgcggtg acgggatcga
    15601 gccccagcag cgactcgtac atgaacgaga acacggtggc gctgaagaca ttgttgttga
    15661 gatagtagtt gaaactctgc ggccaggggc cggcataggc cgtgatctgc ccgccgcgcg
    15721 ccgcatgggg gctggcgacg ggatcgccgc ctcccgccgt gagcgccaga aaagaaagag
    15781 ccgccagcag gtatttcatg aacatcctcc ctcgatcaga gcacttccag aattccggcc
    15841 gcgaaggtga atccgccgcc gaaagcggtg aggcccacct tgttggtgga gcggtgcttc
    15901 gggatgatgg attccagcgc gagcggaatg gtgttggaag aggtgttgcc gtaatcgcgg
    15961 ataaagtaat agactttttc ggtcgggaac ttgatggcct tgcggatggc ctcgatgatg
    16021 cgctcgttgg cctggtgcgg gacaatcatg tccaggtcgc tgacggtgat cccgtcggcg
    16081 gcgcaggcgc gctccagcat catgatcatc ttcttgaccg ccatgcggaa aacgggcagg
    16141 ccgtccatca tcacgtgctc cccgctgttg agggacggca catagaggat ttcgggcgcc
    16201 tcgcccagcg cggacagcgc cggccggctc acccgggcgt tgatgttgcc tttgcggcgc
    16261 tcgcaggaca ccagcgaggc cgtggcggca tctccaaaca ggaaataggt gccggggtcc
    16321 gaggcgttga ggatgggcga cagcgtctcc gcggtgacga caaggatgcg ggcggcggga
    16381 ttatgcgtca gcatgtcata ggcctgctgc agggcataga gatagccgga gcaggccgca
    16441 ttgatgtcat gggcctgcat cagcagttcg ccgctttcag ggctcagttc cttgaggatg
    16501 cggcaggcca gcgacggcgt ggtggaaagc ggcgtgcccg tgctgcagat cagcgcatct
    16561 atttccaaaa tgtcgatatt ttcggccttc agcagtttcc ggcatgcggc aacagccagt
    16621 gtcagcacac tctcgccctc accgatccag tgacgcttca tgatgccggt gcgctggacg
    16681 atatcgtcgg gagaccatcc ggggcagcgc ttcacgagtt cctcattggt catcacgcgc
    16741 gaacccatct gcgaacagat ggatgaaaga atgaccggcc gcgcttcggc tttcgccgtg
    16801 acggccgctc tgacgggcgc ggccgcccgc ttgttcagga tgggccggcc gggattgtct
    16861 tcggtgaccg gtcgggcgga tgcgctttcg gcggtgtgga ttttaaccag cggggtgccc
    16921 acggtggcgg tgtcgccctc gttcaggaaa atttctgaaa cccgcccggc cgccggggcg
    16981 gcaatatcca tcgtggcctt gtcggcttcc accgaggcga tcatggcgcc ggtctcgacg
    17041 gggtcgcctt ttttgacgtg aagccgggtg attttaacgg tctcgtcgga ggggctggac
    17101 ccgatcgcgt tgacgatgag ttcgttgcct tcctctttca cctgctgttc ccatgcgatg
    17161 tcccagtcca gcagcgccgc ggctttttcc atgacggact taaacgaggg cagggtggcg
    17221 agctgcgccc cgaagttgta ggggacatag gcgtcggctg tggtcacgcg gcccgcgcgg
    17281 attttcccgc cggacatttc gctgaccgtc gccgcgattt cgccggagag gctggcggtg
    17341 tgcgtgtcct catccacaat caggagaaca ccggttttgg ccgcggccgc catcacggtc
    17401 tcgacatccc acggaatcat cgtccgcagg tcaatcaggc ccgcgctgag gccggcctct
    17461 tccagcgcgt tcacagcccg ctcgcacagc ggcatggtgc tgccccagct cacgaaggtg
    17521 atgtcgcgtc cggtgcggac cacgcgggcc ctgccgacgg ggaccaggtg ctccccgatg
    17581 tcttccgggg tcgccacggt gcggtcgttc agcaggtttt tcggatagaa gaagatggac
    17641 ggccggccgg aggcaaacgc ggcattgagc aggcccgcgg catcggccgc agtggagggg
    17701 acaaagacat ccacgccggg gatcttggcg gccatggaaa ccggagtctg ggcatggaac
    17761 ggtcccagcc ccgggcggta tcctccgcag atacccatca gaatgaccgg cgcctcccag
    17821 tcgccattgg tccgccagtg catgcacccc atttccgaga agagctggtt gtaggcgagg
    17881 ggcatgaaat ccgcaaactg caggagcgcc accggctgct caccggccag cgcgcgcccg
    17941 atggacacgc ccgcaatcgt ggattcactg agcggcgagt tccgaacctg gtccgggaac
    18001 tgcgtgctca gaccgcgtgt cagtccgaac acgtcgccct tcggatcttc aatatcctcg
    18061 ccgtacaggc tgaccctggg gctggacgca agctggttgc gcagcaccgt ccgcatggcc
    18121 tcaagcatgc tcagtttcct gtcgccggtg ccgcggtatt cgcgcgcggg atcggaaacc
    18181 tcgggcggca gggccttctt cacatcgcgg gcgtcggccg gattgcttcc ggcgcgggcc
    18241 tgctggagct ggcgctccag cgtttcgtca acctgctttt cgatcggggc cagctccact
    18301 ccggcttttt ccagcgactc tcccagaatg cggacgggat cggcggtttc cctgatgtgc
    18361 gcaatttctt cagcgctgcg gtacatccgg tgatcgtcgg agttggtgtg actggtgagc
    18421 cgctcggtgc gcatgaccac aacctgcggt ttgcggtccc tgcggatggt ctcaatggtc
    18481 ctgcccagca cctcatagac cgtttccgga cgggtcccgt ccagccaggc gatgggtctt
    18541 cccagatact cccgcgcctc gcctccggga agcgaataaa aggtcctgcc cgacgttctg
    18601 gaggacaggg caaagctgtt gtcctgaatg aggaagagga cggggagctc gctccgcacg
    18661 gcttccgcga gagcctccag cacctctccc tcctgcgtgc atccgtcgcc catggagcac
    18721 agcaccacgg gctgttccgg ctgcgccttc acggcggcgg ccaccccgca cgcctgcagg
    18781 gcgctgtttc caaccaccgt gggcgaactg agaatccgga gcgccctgca gctggggaat
    18841 ccgggcatgc gccgaccggc ggagttcgat tccgtcttcc ccagcagtcc gtaaagaata
    18901 gtgagcggtt ccacgccgcg cgccagcgcc agcgcctggt cgcgatagtg gcagtgaagc
    18961 cagtcgtact ccttcagatg cggggccagc atggcggttg cctcatggcc gcagctcggg
    19021 acatagaagg ggacttcccc ctgatgggca agcgattccg ccagctcatc cagcttccgg
    19081 ctggttagca tgacccggta taattccgtg aacaattctg tattcatggg tcgaaaactt
    19141 ccgcaaaaca agcccgatac tactttgaat cccagttgag gtctactaga ttatgcgcaa
    19201 atggaaacgg cggcatattc gcgcgccgcc gcgccggttc agccggggaa acgtttcccg
    19261 atcaggctgc ccgcgccggc ggggcccgtg accattccgt gttcaacggt caaacttttt
    19321 ccgccgcgct ccacgctgaa gcggggggtg tgtccgaatg tccagtccca tgaacggtgc
    19381 ttcgcataga tgtcccggaa atccggatgc tggcagatgt ccgcggcatg atggattctc
    19441 cgggaatccc cgaaggagcg cctgatggtt tcggcggcct gatccatggt aatcccgggc
    19501 aggaaatcct tcaggctggc cacgggcgag gggatggatt ccacggcttt tgaatcaaag
    19561 cattccttgt gcggcgtgag cgcgcggcgg agcgcatcca ggtcggcgct gaccagaagc
    19621 gtgcagtgat gaagcgccat gttcttctgc aggcagtagg cgctgccgga tatcttccgc
    19681 tgctgtataa agaggctgtt gccgccgcga cgggcctcca tgcccagact ggcaatggcg
    19741 cggagcatga ttgcagacaa atcatccgga ttgtacgctg tgcgctgcgt gatcaggcag
    19801 cagttgagat ttccgggatc atgatagacc gcccctcctc ccgaaatccg ccgggcgatt
    19861 gtcatgtgca tgctttccat gaattcaagg tcgcattcct tccacggatt ctggttcttg
    19921 ccgatgatca ctgcgggaag gctgcgccaa agaaaaagca cgggtttgtc cggggcctga
    19981 cgcatcagca tctcctcgat ggccagattc aaatgcggat cgcgatagtc cgaaacaatg
    20041 atcatggaag aatgccgtcc tcctccagcc gcgcccgcag cctccgggaa aattcgggcg
    20101 cgccgtattt caggttcagg tggtcttcat cgcggaacat ctggtgatta tggatgaaat
    20161 cgggttccat ggagaaatca taataaacgc cgccgtattt ttctgccagc tgagccgtgg
    20221 cggccctttg ttctgcgaca aggtcgggat tgaagagttc tgtatagcga tagaaatacg
    20281 gggggggtga agaacaccac ttgaatgtca tgtttccgta aatccttgag agtgcgttcg
    20341 accgatttcc gggcatcctt tgccagatgg cggtggtttt tccccatatc gagaatcagc
    20401 ctttgatgtt cagccgcgcg attggtttca gagagactga tcagttcttc gagggtccgc
    20461 ggctcaaaaa gtccgggggc ggtggaggcg ccgtcatgtt cgcggagata aacacgttct
    20521 gcggccggcg gattcttgta gagcagttca aaaagatagt cgcgttcagg ccggatcaca
    20581 ttgatcagtt tgcccttgat gaagtttctc cagtcaccgc gaatccaggt ccatccgggg
    20641 cagtaggcat aggtcttgat gcggtttgag tttctgtcct ggtcatcgga tgcggcattg
    20701 tcgaagtaaa atgtgaaata ggatgccgga tagaagacaa ccttcagatt cgggaggcgc
    20761 gggatgagat gtctgacaat gtattccgtc tcaaacgcgt cgcccccgcc ctcccagacg
    20821 tgatagcccc gaatgccgag tgtgtcaaaa tcaatacccc ggcagtgcga gtgccccacg
    20881 gccagggcct cgatttcatg gcgctgccgg aacaggttgt cccagcgggt tttcacggca
    20941 ggatagatgg ggtcgcggct ggcgccgaag cgcgcgagtc ccgcgagcac gagcagcagc
    21001 ccggtggtga aaccggcggc attcaggatg tggcggagtg ctttcattag aactggaaat
    21061 agatgaattc ggtgatgttg aaccggccca gaataatgat ggcggcaatc atgatccagc
    21121 aggcagccca gcgcagcggc agcggccagg tgcccagcat ctgccgcatg ctcccgtgcc
    21181 gctgcagcag gtgaatgatc tccatgaaac cgatcgcgcc cgccgcaagc aggaaatcct
    21241 ccagcgtctg gcccaccagc agcgggcgga gaacggcgtc tgtgtccagc gcgatcaggt
    21301 tcccgaacca gtttggaatg ccggagaaca gatgccgcac catgtagaac gcgtcataga
    21361 gccgtccggc acggaagaat atccatgcaa aacagaccag cgcaaaggtg acggccacgt
    21421 tgatggtttt gtgcaggcgg ggcaggcggt tcagatgcgt cagcgcgcag aacttttccc
    21481 ggagcccggc ggtccagatg gcaaagatca ggtagaagcc gtgaagcgcg ccccacacca
    21541 caaaggtcca gttcgcgccg tgccagagcc cgctgacaag aaaaacaagg aagagattaa
    21601 agtgccagcg cggcttcgaa acgcggttgc cgccaagcgg aatatacaga tagtcccgga
    21661 accaggtgga aagcgagata tgccagcggc cccagaattc agaaatggat ttggaaaaat
    21721 aggggcggtt gaagttgtcc atcagattga agcccatcac ctgggctccg ccgatggcaa
    21781 tatcggaata tccggaaaaa tcgcagtaga tctgaaaggc gaaaaacacc gtggccatga
    21841 tgaagctgac cccttcgaaa tcgcagggcc gctggtacac cgtgctgacg aacaccgcca
    21901 gccggtcggc cacgaccagc tttttgaaga acccccagag catgagcttc agcccgctgg
    21961 ttgcacggac atagtcaaag ttgtgtttct catagaactg gggcagcagg cgggagcagc
    22021 gttcaatggg gccggccacc agctggggga agaaactcac atagagggcg aaaattccca
    22081 ggtggcgttc ggcctgctgg cgtccccggt atacatcaat cgaatagctc agggtctgaa
    22141 atgtgtaaaa cgagatcccc accggcagaa gaagcttgaa ggccggcaca tggtacatga
    22201 ggttgaagtg ctcgaaaatc gcccgcgcgg agtcattgaa gaaattgaag tacttgaagc
    22261 tgaacaggag gccgaggttt ccggccaggc tcagcataag ccatgctttc cgccgccggc
    22321 tgccggaagg cgcggatacc attcgcaggc ccgcggtata gtcaatcagg gtggagacga
    22381 taatcagaac aatgtattcc gccttccagc acatgtagaa atagtagctg gcggccagca
    22441 gcagcgccca gcggaagcga tgggggcaga ggaagtaagc cgccgtcacc agcggaaaga
    22501 aaaccagaaa ctcgagagaa ttgaacagca tatgttttac ctgcagttca ttctctcccg
    22561 cagctgtttt gaaaatgcgg gcgcgccgtg ccggcggttg agatgatctt catccatgaa
    22621 atatttcgtt tcaaaaacaa aatcgggatt ggcggaaaaa tcgtagtatt ccacattgtg
    22681 cttcctcgca agcgctttca tgttgcggcg gaaggtctcg accatgttcc ggtcggccag
    22741 ttccgtgtag cggaagaagt agggcggggt gaagaagacc gggcggatat cctgcgcctg
    22801 aaggaagcgg atcgtccgtt caagacaggc ataggtgtct tccggcaaac ccggacaatt
    22861 ctggagcatg tcgttcataa gcttcgcatg ttccactatt cttgcttttt ctgcacgttc
    22921 aatcagcacg ttaatattcc ggaaaccctt aaactggcgg tcccggttcc ggcttccgtc
    22981 gcggggattg gattccttcg ctggccgcgg ctgctgtccg agcaggcgaa gcagggtggt
    23041 atacccgtgg tcgggccgga tcaggttgat cagccgggct ttcacaaaac tttggaagtc
    23101 tcccggaatc catttccagc ccggaaggta ggcatacgct tccacgcgct tggacttgcg
    23161 gtcgagaatc gtggccgccc ggttgtcggc aacgaatgta tgaaatgata cggcataata
    23221 aaccgtgcgc accttgggca gccggggaat cagggttttc aggtgaagct cagtttcaaa
    23281 gacatcattc ccgcccagcc agatatggta tccccgctca ccgagggcat cgaagtcaat
    23341 ggccttgcag tgagaattcc cggccgcgac cgcttcgatt tcattcgcct gccgcagcag
    23401 gttctgcatc cggaatttga tgtcgggata gaccggatcc atccggctcc acagggcggc
    23461 gcagaacaga acaattccga tgagcccggc cgtgaacaat atactctgct gcagttgttt
    23521 catttcagaa ctgaaaatat ataaactcca caacttcaaa gcgtccgaaa acaaggatag
    23581 tcatgatcag gctgtagtag atcagccagc ggcaccagac cgggcgttcg gccagaaatt
    23641 ccgagatgcc ctgcctgtca tgaataagct ggaccgtttc catcaccgtg acagccgcca
    23701 ctcctatgag cagttcccag agggtgtggc cgaccaccac gggtgcaagg gcggcccgtc
    23761 gaagatgaaa gacatcgttc agccatgccg gcaggccggt cggcaggttc cggatgatgt
    23821 gcacagcctc ccggatgtcc gcggctctga agaatatcca tccgaagaag agcagaaaga
    23881 gcgtcgaggc gatattgatg aaccgatgaa aacgcgggtg ttttttcagc ccggtttgct
    23941 cgcaccatct gcgccgggtg ttgaaactga gatattccag aattagatag gttccatgga
    24001 gtgccccgca cgcaataaaa gtccacgcgg ctccatgcca gaggccgctg atcatgaaca
    24061 ccagcagcag actgatcacc caccaccgtt tgcttgtgct gcggccgcag agggggagat
    24121 acagataatc acgaagccac gtgctaagtg aaatatgcca gcgctgccag aattccgtga
    24181 tggagcggga aataaacggg cggttgaaat tgcgcatgag atcaaatccc agtacgcgcg
    24241 cggcgccgat ggcgatgtcc gagtagcccg aaaagtcaca gtagatctgc cagccgaaaa
    24301 gaatccccgc cacaatataa ctgatcccct gccagttctc cggctggtcg aacaccgcat
    24361 tcacgaacag cgcgagccgg tccgcaatca ccagtttctt gaagaaaccc cagagcatca
    24421 gacggagccc gctgcacatg cggtcaaaac ggaagtggtg cactgcgctg aactgcggca
    24481 gaagccgcgc ggcacgctct atcggcccgg ccaccagctg ggggaagaag gacacataga
    24541 gcgcaaattt cccaaaatgt gcttcgggcc gctggcttcc ccgataaacg tcgatgagat
    24601 aactcatggt ctggaatgtg tagaaagaaa ttcccaccgg cagcagcacg ttgaacgcgg
    24661 gcactccgta aaaaagattg aagtgattga agagcgtccg gaacgaatcg ttgaagaagt
    24721 tgaaatattt gaacgcaaac aaaatggcga gattcacgcc caggctcacg gtgaggcaca
    24781 gatgcctgcg ggaatcgttc tgcgggagtg tcccgatgca cagcccgccc ccgtagccgg
    24841 cgagagtgga cgcgagaagc agcagcccgt attccgcctt ccagcacatg tagaaatagt
    24901 agctggcggc cagcagcagc gcccagcgga agcgatgagg gcagagaaag taagccgctg
    24961 taaccagcgg aaagaataaa aggaactcca gcgaattaaa cagcatgccc gatttctatc
    25021 cctggttttc cccgggtttc acagtgattc ctcatgatgg gaacggattc tctgaaaatc
    25081 cttgggcgtg ggcaagtcca taaagacggg tttgttgtct cgatgattgc catgccgggg
    25141 ggcatcgcga tagagtggac agcatgaaga ttgcttttgt gaaacagaaa tatgtcccct
    25201 ttggcggagg ggagctgtat ctcgaaaggc tggccggggc ctgccgcgac cggggggacg
    25261 aggtccattt gattactgcc gcctggccgg ggaacagagt ttctgctttc actgttcatc
    25321 ctgttcctgt tcgaaactgg tctcatgcct cggtggcgag gtctttctcg cacaatctga
    25381 aggagatcat ccggtcggtc gggttcgatg tggtcttcag tctggaccgg accgaacagc
    25441 agcacatctg gcgtgcgggc gagggggctc atccggtctg gctggaccgc cgcggcgaat
    25501 ttgagccggc gtggaaagtc aggctggcgc gtctgtcatc gcggcaaagg accctgttga
    25561 agctggagag gcgatgcgtg agccggaccc ctgtgattat cgcgaattcg cagatggtga
    25621 aaaatgatct cgagaactgc tatgatcttt ccggaaaacg cgtggaaata attcacaatg
    25681 gcgttgattt ttcccgcttt tctccggacg gacgcgaaga ggcgcgcacc caggtgcgct
    25741 cacgcttgca ggtgaatccc gacggcccgc tgctgctgtt tgccggaagc gggtttcgca
    25801 ggaaggggct cccggaactg atgcgtgcgc tgcgtggcgt gcccgatgcg gtcctgctgg
    25861 ttatcggccg ggacaaatcc gccccctgga ttcgtttggc tgcgagtctg ggggtctctg
    25921 gccgggtgcg ttttctggcc ccatccgaat ctctgccgga gctgtatcgc gcggccgatg
    25981 tgacggtgtt cccgacatgg tttgattcct tcgggtttgt cggactcgag tccatggcct
    26041 gcgggacccc gctggtcacg accccttttg ccggtgtatc ggagctggtc cgcgaggggg
    26101 tcaatggcgc ggtgatcgcg cgcccggatg ccgccgatga actggcggcg gccatacaag
    26161 accaggtttc ccggaaagac aggagccggg ccatcgcgga ttccgtgaga gaatattctc
    26221 tggaaaacaa catccgccga acccttgcgg tcatagatga caccgcgttc agccgcaaat
    26281 catgagcttt gccggacatg tccgccagcg aaagcgaagc acgggatgtt cagccgcgac
    26341 agctcctgcc attcgattga gaagcgtgaa ataatattga tgaaacattc tgctcggcga
    26401 atttcgcaga atttccgggg tcagcccgct tccggccgct tttgagatgg cctccttgag
    26461 cgtccagact ttgtagaatg cccgcacgcg ctccggttcc ggaagtttgc tgatcctgtt
    26521 tatttcttcc ccggaacagg atttgagcat catgcgctgc aggtctctga cgggcctttc
    26581 ttcttcaata tcaaccccga ccggccatga ttttgccagg gcgaacagca gatcgctccc
    26641 ggtatgggaa acgctgaaga acagcgggcg aaggccgtcc cggctgaccg gcatcatcgg
    26701 ctgccctccg gcgctgtact ccagcgcgca tggatacagc ctgcttccgg tatgcaggcc
    26761 cagcagccgc ctcaacaggc cccggcggat catgaaactg ttctgcggct cctgccgcag
    26821 gaatcttccg gcccgggcgg catcatccgg agtgagcctg ctgcgggccg gttcgagcag
    26881 gctgtttgtg tccgacagat caaaatacca aagctgaata tcgcccggct gcagcgtgtc
    26941 ggtttggaca ttccactctc cgcgcgtgaa gcggggctct atgcatgttg aatccggcat
    27001 catcactcca gaataatcca cagttgatgt ctgtgatcaa gcccggggtg atgggaactg
    27061 gacacacggt tcatctttga gtatgttccg ggactttctt ttggagcagg atgaaagtca
    27121 gaggggaata caggagcatc attgacgtgg ttgaggcgct gacggcgacg gatcccggcg
    27181 ctgcggcatg cacctttctt gcggacggac tgaacgaccg gtgtgcatgg acccggcgcg
    27241 atttgtacag ccgcgcctgt gcggtggcca gagttctgtc ggaccgcaaa gcccgcggcc
    27301 agccggctct gctcctgtac tatcccggcc tggaatttct tgcggcgctg ttcggctgtc
    27361 ttttctcggg ggttgttgcc atcccggcct atcctccggg catcagcgcg cgttcctcgc
    27421 ggcgaattga gagcattctg gaggatgctg gttcgtcact gattctgacc acatccagaa
    27481 taagggaaac gcgccgcaca agcatggacc gtcactcggc tctgccgcgc caggcctgga
    27541 ttgagactga actgattgca ttggactcgg ctgaagactg tgttcctgtc cgggcgcagg
    27601 aaagcgatgt ggcctatctg cagtatacct ccggctccac atcaaccccc aagggtgtag
    27661 ttgtcaccca tggcaatctg ttccacaacc tcacctgcat tgaccggaca ttcaatgttt
    27721 ccggcgggtc cggaattgct tcctggatgc cccactttca tgatttcggc cttgtgttcg
    27781 gtcttcttgc gccgatcttc acgggaaagc cctgcactct gatgcctccg gcggtttttg
    27841 tgcagaatcc gtggctgtgg ctgaaggcca tctccgacgc ccgtgccagt catgctgccg
    27901 cccccaactt tgcctatgac ctgtgcaata cccgcataac agatgcccag ctggaaacgc
    27961 ttgatttgtc ctgcatcacg gcgctgctta atggcgcgga gcccgttcgc gcatccacgc
    28021 tggatgaatt cacgcggcgt tttgcggtct gcggattccg gcgcgaaatg ttttgtcccg
    28081 tgtatggcct ggccgagatc accgtcgggg cggtttgcac ccgttttgat gaaccgccgg
    28141 tctgtttgtc cgtggatgcg gacgcgatcg agcagcacca tgctgtgccc gcggagggga
    28201 atgctcccaa gagccgcctg ctggtttcct gcgggcatga agtgttcgga caccgaatgg
    28261 aaattgtgga tccggtggct cttgtgcgcg taccgaaggg cggggtgggt gaaatctggc
    28321 tggattctcc cagcgtgtgt gcggggtact ggaaccggcc ggatgaaacc cggcaaacct
    28381 tccaggcttg tctggcaggc acgaatgagg ggccgttcct gcgcaccggc gatctggggt
    28441 tcatgcacga cgggaatttg tatatcaccg gccgcattaa ggacctcatt atcattcacg
    28501 gggttaacta ttatccgcag gacattgagc tgtcgtgcga gaaggcgcat cgcgccctca
    28561 aagcgaatgc cggcgcggcc ttctccgtgg agcgcggcgg atcagaaaag ctggtggtgg
    28621 tgtacgaaca ggaccgcacc gtgaaagacc cggatatcgg ccagatcgcg atggctgtgc
    28681 gccacgcggt gtctgaagag catgagctgc agatttatga cttcgtcatg gtgcggccgg
    28741 gcacgattcc caagacttcc agcggaaaaa tccagcggca tgcgtgcaaa gcggattatc
    28801 tgaacggcag cctgaaggtg gttgcatcca gccggcttga aattgcggcc gccggagaaa
    28861 gaaatatcgc gccacccgct gatgaaatgg agcggcagct ggcgcggata tgggaggaag
    28921 tcctcgagat atcgccgatc ggggtgaccg acaactattt cgatctcggg gggaactcgc
    28981 tccatgccct gcgtatcatc ggtaaaattg gagagacaac cggccgtgaa ctgcagctga
    29041 ccgatttgtt tcagactccg acgatacgcg gagtggctga gctgctgcgg cgggcggacg
    29101 aaaccgccga gttgatcgcg ctggtcccgt tgcagccggc ggggacatgt actccgtttt
    29161 tctgcgtgca tccaggcggc ggaatggtca tgtgcttcaa tgatctggcg cacagcgtcg
    29221 gaaaggatca gccgttctat gctctgcagt cgcagggtct ggatggccgt cgttccccgc
    29281 tcaagcgtat tgaagacatg gcctctctgt acattcgcga aataaaaaca gtgcagcctg
    29341 ccgggcccta tcggctgggg ggaatgtgcc ttggcggggt cattgcctat gaaatggcgc
    29401 agcagctggt gcggctggga gagcaggtgg aattcctggg agtactggat acccgtcgtc
    29461 cgcccggctg gtacagacct ctgttccgcc agcgcagggt gatcaaggag tggctgggca
    29521 tgatatccca tgcgggacag aagcgtgtgc tgaagcggat atggctggca aacgagaaag
    29581 cccgcaaccg gtatcgcccg gaaccgtacc ccgggaaaat tacgcttttc tggagcgatc
    29641 acgagggtga tgagcaggcc gaccggcagg aaatgtggcg cagacttgcg ctgggcggtc
    29701 ttgacataat tcccatcgcg ggggcatcgc atcgcgccat cctcgcggcg cctcatgtgc
    29761 agacgcttgc agacaggatc gtgtgctgcc ttgaaccttt caacgggatc cgctcatgag
    29821 tgtgctggct gtaaacgtga tctgcacgtc atattcaggc aacacgtggc tcaatttgat
    29881 gctgggcgcg cattcgcggg ccttctgcgt gggcgaatac aaaaacatac gcaagaccgg
    29941 ccgggtgaac tgcatgcttc acggcgcgga ctgcccggtc tggagccgtt ttgatgcctc
    30001 ggcgcaggag catccgtatc tgcagctcag ccgcctgacc ggaaagaaca ttctggtgat
    30061 cagcaattca cttgattatc tcccggccga tcccggaccg gggattgagc agcgattcat
    30121 tcatctcatt cgcgacgggc gcgcggtggc cgccagtttc aggcggaagc accgcggaat
    30181 gaacatctgg caggctgccc gccgctggcg gcacgaccac cggaaaaatc tgcgccgcct
    30241 gagccggcat ccggcagaag ccgttctgcc cgtgcgctac gaagagttga agtccgaccc
    30301 cgagagggaa gtgcgccgca tttgcggatt cctcgggatc ccctttgaac cgtccatgct
    30361 ggaatactgg acgcgggaca tgcattatct gggcgggaat cagggcacgc tgctggcgct
    30421 cgcgcgcaaa cagggagggg atctgcccga gtcctcgcgc agggagcagg gccgggagca
    30481 gcgtgactgg aagctggaat tctacgccgg gtccgatcct cagaagttta cagacgaccg
    30541 ctggaaaagc gatctgacct ccattcagct tgggatattc aatttggccg ccggcggttt
    30601 gaaccgcaga ctgggctatc agtgattatt ccactgtaat gcccagcgcc gcgagggtct
    30661 cgtacgtcag cccgccttcg gcaatgtcac ggtccttctg gaactgtgcc accgcctccc
    30721 gggtctggcc cccgatcacg ccgtccacgc cgcccgtgtt gaagccttgc tcaatcaggg
    30781 ctgtctgaat ctggcggatg atgtccggag aaaggttgga ttcgcacagc acgcgcttcc
    30841 agagcatccg gccgggttcc accattacct ctttcgaaac ggtctgatac accgccggaa
    30901 taattttgcg ctcctcgcgc tccggtgcgg tcatcttctg caccctcacc gtttcgtatt
    30961 cggccggcac ggtcacatcg cgcgttgttg cgggtttggt cattacgtgc ttgcggatgg
    31021 tctcatattc agcggggacc tggatttccc tgacactggc cggctccgcc agcacgcgtt
    31081 ttttaacctt ttcgtatacc ggggggagtt ccaccaggca cagaatctcg ccggtggcgt
    31141 tgtccactgt ggtgatcagg cccgtgccct ttttccactc gcgccgcgcg ggcttcacca
    31201 tcacttcctc ctcgacccac tcatagcgcg cgggaatctc ctcggtgcgc acagaggcgg
    31261 gagtcaccag cacttcctct tccacccatt catattctgc cggcacaacc tcaagctccg
    31321 tggaagcctc cttcacaagc acttcttcct cgacccattc gaactgcgcg gggatgacca
    31381 cgatttcttc ggactccggc tggagaagca cctgctcggt cacggtttca tatcggggcg
    31441 gaatgtgaac tctggcatag cattcaccgg gccgggcgtc cgggggaagc gcctcgtctc
    31501 cgccggccga cggttcagtc acggcgggtt cgatctcctc gttgaacact tcgggagggg
    31561 gaatcgcggc cgggtccggg agggtttctg tctcatcggc gggtccaacc ggctcttctt
    31621 cggcataggc cggaacagcc ggtgccggac ggaccgctat cgtgtgcgcc ggagggacat
    31681 catagacaac ctcggccggg ggcacgtccc agtcggtctg ctgccccgcc tttattttat
    31741 aggcattgga gggctcaaaa aattcgcatc ccgccagcag catgacggac agtgcggcgg
    31801 ccgcgcagaa gaccagagac ttgtttttca tgttcggata ctccttaaca aaatgtggcc
    31861 taacattact attccgggag cccgacgcaa tgaaaaactt gccggtcagt ccggcggatg
    31921 ggcccgcttc tcaaacccca tcagagcgcc gccggttatg gccgcgccgc cgtccacggt
    31981 gagaatctga ccggtgatct tctgtgcata gggccccagc aggaaagtca cggcatccgc
    32041 cacggcctgc ttgtccttca gcgtgtccca cgggagcggg ctgaccgttt cccagaggct
    32101 ggtcaggctt ccaaagcccg gaattttgct ggccgccttg gtgaacagcg gcccggcgga
    32161 aacggcattc acgcggatca ggtcgcggcc gtgccgtcgc gccagcgcgc gcacaagcgc
    32221 ctccagcgcg gccttctgca cccccatcca gttgtagtag ggatacacat gcgcggtgtc
    32281 gaagctcagc gccaccaccg aaccgccatc cttcaaactg ggctgcgcat gccgcagcac
    32341 cgtggccagc gaaacacagc tgatgtggtg cgagagcagg atgtcatcaa tttcgcttgt
    32401 gtggaactcc tcgccgagac aggtgttggg attggcatag gcaatggaat gcaccagtcc
    32461 gctcagcggc ccgaggtctg taaaaagctc ctgcacctcc tcgtcaaccg tcacatcgca
    32521 gaaccgcaga tcgagggctt cgatttcctg ccggctgagt gcttcggcgc ggtccagaaa
    32581 gcgcttcttg aacacttcat tctgaaccgt gtagatcacc cggccgccga agcgctcaat
    32641 ggtcttcccg atggcaaagg ccagcgaatc ggcatccagc agccccatca ccagatatgt
    32701 ttttcccgcc tcaatcatgg catgctctcc attcaaacag cacacagcct actacacaaa
    32761 tcgccgtttg aacagccgtc cggccgcaaa agttccaacc attggaactt ttcgtcaaaa
    32821 aacttccaat cattggaact ttttccggca aaacttccag tcattggaaa atgccgcaaa
    32881 aatggatttg cgaaacagcc ggtcagccgt catgctttgc cgaaatgaca ccagctccag
    32941 cagaacggaa atggacggag tgcggccccg ggccccgggc cgtgctctat tttcttgcgg
    33001 cgatcacggc cggcctcctg attgcgccgc cggtgttcaa tctggtcacc tggctgggcc
    33061 gcgagttcca gtggctggca ggcctgcggg aaaccgcatt cgaaaagctc ctgaaccggt
    33121 gtgtgatcct ctgcgtcatg ctggcgctct atcccgcgat acgggtcacc ggcctgaaca
    33181 gctggaaggc gctgggactc tcgcgggacg atcgcgggct gcgtctcttc tggatcggct
    33241 ggatcatcgg aaccgtttcg atggtcggcc tgatgctgac ggggtgggct tcgggcgcga
    33301 tgtactatct tccgggccgc gtggactggt cgctggccgg gaaggtggtc agctatatgt
    33361 tcggcgccgt tgtggtgggg gttgttgagg aaatcctgtt tcgcggcgcc gcgctgggca
    33421 tgctccgccg cgggctgcgc ctgcttcccg cggccctgct gctcagtttt gtttttgcgt
    33481 tagcgcattt cgcgactccg gaaccggccg tcggcgtggt gcgcggccac tgggattccg
    33541 gcctgcggat gctcccgtac ctgatttctg tcgaagacat gcgctgggaa tatgcgttca
    33601 tgttcatcac ggtgtttttc atgagcatga ccctgtgctt cctgtacgcc gcgtacggga
    33661 atctctatct ggccatcggg cttcacgcgg ggtgggtctg gctgatgcgc atcggcgggc
    33721 acctgctgga gcgcgatgac aatgtgatga tggtgctgtt cggccccagc atgtctgtgg
    33781 ccaagagcgg catggccatc attgaagtgc tgctgttttt cgcggcggct ctgtttcttt
    33841 acgggcgcaa acgcagaacc gttccggatc cggcatgagc ctgaaccata tcctggacat
    33901 ggtatatccc cgcgcctgca tcggatgcca tgcccgggcg ccggaaaatt caccctatct
    33961 ctgctgggac tgccagtcct caatcatgtt cgtgcagccg ccgttctgct cgcagtgcgg
    34021 cgatccggtt tcgggctata ttgaccatga ttacatctgc tccttctgcg cggaaacccg
    34081 tccggccttt gaccgggcgc gttcggtggc ccgctatgag ggcgtggtcc ggcagatgct
    34141 gcgcgatttg aagtataacg acgcgatctg gctggtcggg gacctcgcgc gcatgctgga
    34201 gatctgcgcc cgcgcccatt acgacctcgc cgccgttgat gtcgtctgcg ccgtgcccct
    34261 ctacggagcc cggcagcgcc agcggggcta taatcagtcc cgggtgctgg cggaggcgct
    34321 cgcggaaaga atccacaagc ctttcatatc aaagggattc cggcgcatcc gcccgacgcc
    34381 gacgcaaacg gatttgacag caggggagcg gctggctaac gtgaaagatg ctttttcggc
    34441 cgtcagccgc ggctggattg aagggaaaag aattcttatg attgatgatg tcatgaccac
    34501 cggagccacg gtgcatgagg catcccgggc actcaggaaa tgcggcgcct ccagaattta
    34561 tgtgctgacg gcggcccgcg gttaacgagg tgataagatg tttccgtttt tcaggaagaa
    34621 aagctcaacg ctgacggcaa gaaaaagaga tgtccccgac ggattgtgga caaagtgtcc
    34681 ctcgtgttcg gaactggtct accggaagca gatgcatcgc gatcacggca tctgcgcaaa
    34741 atgcggcttc catttcccaa tcggcgcggc cgaacgcatc gaaatgctgc tagaaaacaa
    34801 gtccttcgag gaatgggatg ccggcctgga atcggtggat tcgctggagt tcaccggaac
    34861 ctcctcctat gtctccaagc tggaatccaa catgaagaag accggccgca aggatgcggt
    34921 gatctgcggc agggggcgca tcggcgagca cgccgtggcg cttgccgtca tggattttga
    34981 ttttctggcc gcgagcatgg gggccgtggt gggcgaaaag atcacccggc tgattgaacg
    35041 cgccacggcg gagcggctcc cggtggttat cgtctgcgcc tcgggcggcg cgcggatgta
    35101 tgaaggactc ttcagcctga tgcagatggc caagaccagc ggggcgctgg cgcgccatgc
    35161 cgccgccggc ctgccctata tccccgtgct gacacacccc accaccgccg gcgtcatggc
    35221 cagctttgcc acgctgggcg atgtgatcgt ggcggagccg gatgccctga tcggcttcgc
    35281 cggaccccgg gtcatcaagg aaaccacgca gcaggacctg ccggacggct tccagaaatc
    35341 cgaatttctc ctgaagcacg gtctgctgga tatggtggtg aaccgcaagg aactcaagtc
    35401 cacgcttatt cagcttctgg attatttccg gccgcatcgt cagggcgagg ccaaggcgtc
    35461 gtgagcagca cgctggcgtc gtcgctggaa aaacttttcc gccgcaccgc cttcggaatc
    35521 aggcccggcc ttgaggtgac ggaagccatt ctttccgagc tgggcgatcc ccatcgaaga
    35581 actccctgtg tgcatgtcgc cggcaccaac gggaaggggt cggtggcggc catgatggaa
    35641 tcggtgctgc gcgcggccgg aatcaggacc gcattctaca cttcgcccca tctggttgat
    35701 ttcaatgagc ggttccgcct ttgcgggcag ccggtgggcg atgatgagct ggcccggctg
    35761 ctggaagacg tggagcaggc cgccgatgcg gcgtcgcgga agcacggtct ccggcccgcc
    35821 actttttttg aaatctccac ggcgctggcc ttcgaatatt ttgcgcgggc gggagcggaa
    35881 ctggcggtca ttgaaaccgg catggggggg acatgggatt ccaccaatgt tgtcatcccg
    35941 ctggtttcgg tcatcacgcg catcggcatg gaccatatgg aatatctggg acacacgctg
    36001 gaggccatcg ccgccgaaaa aagcgggatc atcaagccgg gtgttcccgt ggtgttcgga
    36061 gccatgccgg aggaggcttc cgcagtcctt ctgcgccgcg caaaggaagc gggtgcgtcc
    36121 gctcttgcgg ctgccggata tgcgtcggtg cgccgcatga aacaggggct ggacggccag
    36181 cgcctgaaga ttgaaaccct gcagacggct tatccgcccg tcctgctgcc cctgcttggc
    36241 gatcatcagc ttgaaaatac agcgactgcc gtcgcggcgc tggaaacgct ggccggccgc
    36301 ctggaaacag aactggccgc cgatgccgtc aaacacggcc tggaaaaaac attctggcct
    36361 gcccggtgcc agattctcag ccgcgaacca ccggtgatgc tggacggcgc gcacaacgaa
    36421 agcggcgccg cggctcttgc gcagaccctg aagggcctca gggaaaaacg tccgctgcgc
    36481 atgattgtca gctttctggc ggacaaggac atggacggct ttctgcggca ttttggacgg
    36541 caggcggatc acgcatgggc ggtgcagctt cactgcgacc gcgcacggcc ggtcgaggac
    36601 atcatggctg cgctccagcg gcatcacatt ccatgtgaat cgcggctcct gccggatgcc
    36661 cttgcggaag cgcgaacctg ggcggtggaa aacaggggga tggtctgcat tgcaggatcg
    36721 ctgtatctgg cgggtgaagt cctgaagctt gagcagggca gattgaactt tatatgaagg
    36781 cggatctcat tatgatctgc cgccggatgt gagaagatga agaaactgaa aatagccttg
    36841 gcctgcggcg gtaccggagg gcacatattc cccggcatgg ccaccgcctg tgtcctgcgc
    36901 gcgcgcggtc acgaggtcac gctctgggtg gccggcaagg atgtcgagag caccgcgctg
    36961 gccggatggg acggacctgt gctcaccgtg aaagcgcgcg ggttcccgca tggagtatca
    37021 cttaaggcgc tgggttcggc ctggcagctg ctctgcgctg tccgcaaatg ccgggcgatg
    37081 atgaagaaag accgtcctga cgtggtgctg gcgatgggca gctatgcttc ggtgggtccc
    37141 gtgggcgcgg cgcgccgcct caggattcca gtggtgctgc acgagtccaa cgtgcttccc
    37201 ggccgcacga atgcgctgtt cgcgcgcggt gcgtccgccg tcgccggatg ttttgaggag
    37261 acccggttct atatgaaacg gaaggatatt gtcctgaccg gcatgccgct tcgcagagag
    37321 ctcgaggcgg cttccgccgt cctgccgtct ctccctccgg agggcgggcc ccgcaccgtg
    37381 ctgatcatgg gcggcagccg cggagcgcgc cgcctgaatg aagtcgcctc gcgggcggtc
    37441 tgcgaattgc accgcaaagg cagtgccgtg gaagtgattc atctggccgg cgtggccgat
    37501 gaaaaagaag tgcgaagcat atacgaggaa gccggtgtgc cccacaaggt tgaatccttc
    37561 atccagaaca tggcgccggt ctatcagaag actcatctgg ccatctgccg ttccggcgcg
    37621 gcgacctgtg cggaactgtc cgccttcggc attcccgctc tactggtccc gtatcccttt
    37681 gcctccaagg atcaccagac agccaatgcg cgcgcggtcg agaaaaagaa ggcggctgat
    37741 gtggtggatg aaaaggacat gacctgcgag tttctggtgg actatctcac aagaatcttt
    37801 tcgtcaccgg accggctggc cgcgatgagc caggcggcca gaaagcgcta cagcggccgg
    37861 ggcgcggagg cgctggcgga tcttgtggaa cagaccgcgg ccgggggccg ggagacgaac
    37921 tgatgccgga ccgcgatgtc gcatctatcc ggctctggct gcggtcgggt caggggcatg
    37981 ctcatttcgc gggcatcgga ggcgtaggca tggccggcct cgcattcctt gtcggcaaag
    38041 ccggcttccg cgtttccggg tgcgatcttg tggaatcccg catcacggca tggctgcgcg
    38101 caaacggcat tcaggtgcac tccggtcacg atgccgccca tattgatcaa tccgtgactg
    38161 cgctgattcg gactccggcc gtgagaccgg atgcgcccga ggtgaagcgc gcgcgggaaa
    38221 agggggtgcc tgtctatctg cgcggcgccg tgctggcggc gttgatcgaa gatcgggcct
    38281 ccatagccgt taccggcaca catgggaaaa ccacaacatc caccttcacg gctcagctgc
    38341 tttctgcctg cggacgcgat ccctctttct gcatcggcgc ggaagtcgcg ggccccggag
    38401 tcgtggcgca tgcgggccgg ggaaaacaga tcgtggtgga ggcggatgaa agcgatggaa
    38461 cggtggccct ttaccggccc gagacggcac ttttgacgaa catcgagtat gatcacatgg
    38521 aacatttcgg gagcgaagcg gaaatggagg actgcttccg cgcctttgcc cgcgcggcgc
    38581 gccggcatgt gatctggtgc gtggatgatc cgggcctccg ccgcgtgatg gctggaatcg
    38641 ggaacgggat cacttatggc ctctctgaaa atgcagactg ccgagccgcc gttgagaaaa
    38701 tgacagcgga tgaaaccacc atccggctct ggtggcgggg ggccgaccag ggcctcttca
    38761 cgtgtgcggc cgcgggcatg cacaacgcgg tcaatgcggc cggcgcggcg gcggcggcgc
    38821 atttgtacgg ctgctcattt gatgagatcc gggacggatt ctgcgaactg catcttgcgc
    38881 gccggcggtt tgaacaggtg tgtgatctga agggggtgcg ggtgatttca gactatgcgc
    38941 atcatcccac ggaaatccgg gcgctcatcg aggtggcgcg cctgcagccg cacaggcgaa
    39001 tcattgccgt gttccagcct caccgctata cgcgcacgcg ggcgctgggc
//
LOCUS       DAOQWR010000005        36357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1345_length_36357_cov_5.888711, whole genome shotgun sequence.
ACCESSION   DAOQWR010000005 DAOQWR010000000
VERSION     DAOQWR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 36357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36357
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_36357_cov_5.888711"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKK36_00870"
     CDS             complement(<1..980)
                     /locus_tag="PKK36_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNR93142.1"
                     /translation="MSMKLLDRIKKGIFIMDGAMGTQIQNFDVPPSAWQDKEGCNELL
                     NLTAPDIIRQIHTTYFAAGSDAVETNSFGSSPVTLGEYDLSAKAREISLAAARIAREA
                     AAAASTPDRPRYVFGSVGPGTKLPTLGQIPYDQLRDGLTVQIEGLLEGGADGILFETC
                     QDLLQIKAGIAAFEKAGGLKAGAPLYVSVTVEQTGTLLIGSSIAAVVAALSALPVNIL
                     GLNCATGPEAMRIHLDYLAENWTGLLACMPNAGMPELRSGQVHYPLQALEFAKKLGSM
                     AREVGLNVAGGCCGTTPAHIEALCNELKGFRAPERIITAPQQVTSVFGPV"
     gene            1139..1309
                     /locus_tag="PKK36_00875"
     CDS             1139..1309
                     /locus_tag="PKK36_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93143.1"
                     /translation="MKKFQSLENLEKKVPTIGSFSLKSSNHWNFLRGLLFGAGMMMTG
                     TSPAAGPSPGVS"
     gene            1446..2084
                     /locus_tag="PKK36_00880"
     CDS             1446..2084
                     /locus_tag="PKK36_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR93144.1"
                     /translation="MKAQSYRMQMTGSRIRGGYRAVLVLALNILTLCFLSSTSAAEGR
                     APGWFRRHFGDVRWEEAVQNSRSPKDVCGFTADRLRYSAESGDVWSDPAEIWNRRSGD
                     CEDYAAVVMDMCREAGFEAWVEVYFPRGRGEGHAVAVGPLPEGRYWVSSFGKYETVSS
                     REEISRLMAGILWCDAGDLWSARMSYESVHGKIARQQQARLSRMAAAASSPF"
     gene            complement(2092..2487)
                     /locus_tag="PKK36_00885"
     CDS             complement(2092..2487)
                     /locus_tag="PKK36_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93145.1"
                     /translation="MTIFSHMSEGLIVVLEDERNFSRFLKMALEERGYEVYCYDNGQD
                     GEEAIKNMCPDLAILDVMLPRKNGLDICMELKRKEKYRNIPILMITGVASNAGRDNSY
                     WQEQTSADGFLCKPISVQELYQTVDKLLN"
     gene            2614..4362
                     /locus_tag="PKK36_00890"
     CDS             2614..4362
                     /locus_tag="PKK36_00890"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNR93146.1"
                     /translation="MAGMDIFFLVFSVLGGLALFIFGMNVMTEGLRTAAGSQLRVILA
                     KTTGNRMLGIALGTGLGFLVQSSAATVMLVGFINAGLMSLLQSIPAMLGANIGTTISM
                     QLISFKLGAYCYFAITLGFIINMVARGVKARSMGRALLGFGLLFLGMNVMSDAIRPHR
                     ETLAPLLLSADGATIKGMLIGVLFSTAFTGIIQSSGATIGMVFAMISAGVITSFQQAY
                     PIVLGANVGTCATALLGSIGTNIEARRSAVSHLLFNIFSAVVGILTAPLFYRYIHLTS
                     GDLIHQAANANTIKMVATALMVLPFAGIHARIVRALTPSRTPAPENSFLDSELIPTPE
                     TAISAAVQELQRIARVCARSMRLNADLLFDPSKKILQAIKLNEGVINEIKTAMKDYLT
                     KLGHRQLSERQAIIILHLDRCMTDIERIGDHIEQIADISWARRQKKSEAIFDEQSFSE
                     MFALYEKALQVLCLVIESLDIKQGDFQDIAKRILAMRDEYITLNIETRSRFIGKIARQ
                     EMPPLCGMYYAGYLSAFERIVKHSKSIALAEQQPQFLIKRGKLGQVAPQAPDYKPPEL
                     VDPNDFLDRLQSERYM"
     gene            4382..5269
                     /locus_tag="PKK36_00895"
     CDS             4382..5269
                     /locus_tag="PKK36_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93147.1"
                     /translation="MKSAKRIFIILVMVLAAILLAAMLFLGPLIKTAVEKGAPAMMGV
                     PVTLDRPGINLLAGKISLNNLVVGNPEGYKTDYAFKLGKFRIDMQMSTLGSDTMIIRD
                     ILIDSPEIVWEAGLGSSNIGTILDHLKKKAPAEKPEAEKEEPKKAGTQVQIDNLLITG
                     AKLKFSAKMMGGQAITIPLPDIHLKELGKDKDGASILDVITEVMNAVVSAIGEALSSA
                     PELLGKGFGAAMGVAGDGVTAAGSLADDGVRAVGGIAADGAKAAGGIVTEGVKAVGEG
                     AGAIIKGVGGLLPGGDKKK"
     gene            complement(5272..5952)
                     /locus_tag="PKK36_00900"
     CDS             complement(5272..5952)
                     /locus_tag="PKK36_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNR93148.1"
                     /translation="MCGRFTLTAKIESVTELLRTAPVSPYEPRYNIAPSQPVLTLLND
                     GSRKLTFTHWGLIPGWSGGPGKWKPLVNARAETLSEKPSFRACLRTKRCLIPADGFYE
                     WPRSASKGPPVYIRMANGGVFAFAGLWDEWQDREGGLIISSAIITTRPNRLLETVHDR
                     MPVILKPEYFEQWISQNLIPDREVEQMLQPFSANEMHMHPVSAKVNNARHDHPDCIAP
                     AAQQSFGF"
     gene            complement(6019..9006)
                     /locus_tag="PKK36_00905"
     CDS             complement(6019..9006)
                     /locus_tag="PKK36_00905"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNR93149.1"
                     /translation="MAREIASNTEQAEVRDDFFDHQSGAFFRKVDWLTFFITFAIAFG
                     VYVYTLSPTVSLEDSGELAVASDYLGVPHPPGYPIWTLVTWFFQWVFHFDSYNGSPNP
                     AWAVGLASAFFGALAAAIIALLVSRSGADILRGLAKETRILGRETESAICLSCGISGG
                     LLLAFSSVLWSQSTIVEVYSLNAFFLALLLILIYRWMCRPQDDKILYATAFIFGLGLT
                     NHQSLLFLVLALLTGLWFRDRRLFRDALSLGFILIAVYLWYSASKINVAGSAEALRDK
                     QVKIAVGFVFLFAPLVFLIMQRSLFTEWKRVLIVFGLLALGVSFYAYMPVASEQNPPM
                     NWGYPRTTEGFMHALTRGQYEKISPMATLRQYGQNQTLFFRQLWAIILNPGGYNSVVA
                     QFTWILSLFVLVPFAFLRKITRQSRAWIWATLTAFLSMTVIFIVFQNPQLDVQTLFIG
                     RVQYIQAHAVFALWISYGLMFALVFLESRAGWLLRQPATICVLLLPLVPILRNAYDKE
                     FVRIIGGCEQNGHTFGWQFGNWQLRGVNGIEDDLRHWLSPGEFEKEWADYPTPGYPPE
                     MGTNAIFFGGTDPGRFVPTYMIYSAKVRRDVFLITQNALADNTYMNVMRDLMGNDIWI
                     PSQQDSNLAFQQYVRDVQEGRIPPGAEVSFKDGRVSVQGVQGVMMINGILAQMIFEHN
                     KYKHPFYVEESYVIPWMYPYLEPHGLIMKINPEPLPGLTPKMVENDRKFWAWYKEYLT
                     SNPKFRRDVVARKTFSKLRSAIAGLYAARRMMPEAEEAFTQAIDLYPLSPEANFRLAE
                     LYMQQRKFTQAKELIQNFLKLDKDNDKVESFLNQIVGAETSENRRMQLEAQLSGGGAT
                     LDAIFELSDVYLQLNMQGSFEQLMRRLLEQPGVPENVYMEIGQRCANAGRIDLLGLAL
                     EKYLAVQPNNDRAWFDYAAVKTMAGRQEEAISALTRAVQLGGEPIRKIAREDPRLEPL
                     RSLPEYQKLIPAPRALSLPRLGL"
     gene            complement(9122..10015)
                     /locus_tag="PKK36_00910"
     CDS             complement(9122..10015)
                     /locus_tag="PKK36_00910"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNR93150.1"
                     /translation="MKAAFSYFRALRPNQWTKNAVVMAAFVFALGDRHQAVSLGQLGD
                     AVGAMLTFCLISSGIYLLNDIRDVERDRLHPTKKFRPLAAGEISVKGSLLLAFASLAA
                     GLAGAGFICPRLALVEAGYVLLQIIYTVWLKHIGLIELFVIAGGFVLRAVAGAVTVDV
                     VFSPWLLLCTLLLALFLALCKRRHEKVIIDATGSDTRPSLGSYNERLLDQLIAIVSTA
                     TIVFYSLYTLWPDTIEKFGTAKLALTIPFVMFGLFRYLDLVYRHEQGGRPEKILLTDI
                     PLIVNILLYGLTVLAVIILHI"
     gene            complement(10012..10746)
                     /locus_tag="PKK36_00915"
     CDS             complement(10012..10746)
                     /locus_tag="PKK36_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNR93151.1"
                     /translation="MNDTVIVIPTYNERENVQQIAAAALNHSPGAHILFVDDNSPDGT
                     GRIADEMASSDTRIHVLHKERKEGLGRAYIAGFHWALERNYQYIFEMDADFSHNPADV
                     PRLREAAESGADLVLGSRYIGGIRVINWPLNRLILSKGASLYVRLITGMPFMDPTGGF
                     KCFRRRVLESIDLDSIRSNGYSFQIEMTHTAWLQGFQIQEVPITFEERREGQSKMSSG
                     IVFEALWMVWKLAARCRFRRSPRKAQ"
     gene            10845..11402
                     /gene="def"
                     /locus_tag="PKK36_00920"
     CDS             10845..11402
                     /gene="def"
                     /locus_tag="PKK36_00920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNR93152.1"
                     /translation="MELKIKTYGDPVLRERARPVDEITPEICDLAEEMLRVMHQQNGV
                     GLAAEQVGRREAVVVIDIPPGADCDKEKNPLNPDAVMPLVLINPEITESSKKKESCEE
                     GCLSFPGLWAEVSRPAEVTVHYTGIDGRPQDMRCRGLLARAVQHELDHLNGTLFIDRI
                     SYARKIALKGRLKKLQEETLESHLL"
     gene            complement(11388..12326)
                     /locus_tag="PKK36_00925"
     CDS             complement(11388..12326)
                     /locus_tag="PKK36_00925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNR93153.1"
                     /translation="MLKVVRHLDTLKTETAPVVLAIGFFDGVHLGHRAVIEAARAQAE
                     KLAAELWILTFEPHPRKVISPETAPALLTATRHKLHIFEQMNVQGCILMPFAPAFRHL
                     TASEFLEMLTEAIPTLRHIVVGSNWTFGHKGTGTADVLKQWALAHGIGTSTVAPVTCD
                     GEPVSSTRIRKAVAGGNLKDASAMLGQPFSIWGTVVRGRGIGRTMGFPTANIRPHNEV
                     FPPEGIYAVTAEIGEGIFHGAGYIGRRPTFTQAGNEWSLEVFFFDLRENIYDREIIVR
                     FAERIRSDRKFDTPAELTRQIEDDVRRAKSILSEQM"
     gene            complement(12320..13057)
                     /gene="truB"
                     /locus_tag="PKK36_00930"
     CDS             complement(12320..13057)
                     /gene="truB"
                     /locus_tag="PKK36_00930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNR93154.1"
                     /translation="MNNRPDTGGLDGLLLVDKPAGITSHDVVHIIRRHFGIKKVGHGG
                     TLDPAATGLLLILLGRGTRLSSQVMSSNKTYEGIMRLGITTDSQDMDGEVTREADCSA
                     ITAEQIAAEMQKLTGDIMQTPPMVSAIKKNGVPLYKLARKGVTVEREPRLIHVFNFSL
                     LDFSPPRAQFRIKCTKGTYVRTLCHDIGEALGCGACLEALRRTQSGSYRVERARTLDD
                     LMQISRADLESLVIPFLQVKQDEQILC"
     gene            complement(13050..13433)
                     /gene="rbfA"
                     /locus_tag="PKK36_00935"
     CDS             complement(13050..13433)
                     /gene="rbfA"
                     /locus_tag="PKK36_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNR93155.1"
                     /translation="MSVRRLERVNELLKREIAGVIYKIGGEEKLDLAAVTVTHVITSP
                     DIRHARVMVSVRDHEQERRHIIDLLTRHRGTIQRHINSDLKLKYTPQLTFELDESIEK
                     GDRILSIISELGLSDDDTEKPDGDE"
     gene            complement(13480..15516)
                     /gene="infB"
                     /locus_tag="PKK36_00940"
     CDS             complement(13480..15516)
                     /gene="infB"
                     /locus_tag="PKK36_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNR93156.1"
                     /translation="MRIFELAKELNISSKDLLAKLRAMGIEAPNHMSALADEQIAQVR
                     EAVGGKPAKPEAAPEKPAAAPEQPEPAPPATPEPEKVEPPAENVYIVKGPIIVKEFAD
                     VLKLKPNQLIAELMMMNIFAAINATIDFKVAQKVGEKHGVKVEQEKAAPPPPPPPPPP
                     SKKKEKKEEEEAKGGEELRPPVVTFLGHVDHGKTSLLDYIRKSSVVDGEDGGITQHIG
                     AYMVEHMDRPITFLDTPGHAAFTAMRARGANLTDIAVLVIAGDDGVMPQTREAIQHAQ
                     AAEVAIIVAINKSDLPGSNPDRVKQQLQEMGLTPEDWGGDIICCPVSAHTGQGMDHLL
                     EMILLQADMLELKANPRQSARGFVIEARLEPGMGPTATFLVSDGTLKVGDAVVCGSTW
                     GRVKALMNDKGHKVRTAGPAAPVKVMGLTSVPDAGSEFIACASDREARSIAESRQEQN
                     RMENLTAPQRASLDNLFSQISENAQQTLNLVLKADVKGSVEAIEQMLQGIKSDKVSLN
                     LVLTGVGNITENDILLATASNAIVVGFHVSAEGGAKAAAKRSGVEIRLYSVIYELFDQ
                     IKEAMTGMLEPEFKQRVIGRAEVRQVFSLSKRGKVAGCMVTDGRATSRAKVRVLRKND
                     VVFEGSLASLKRFQNDAAEVREGQDCGIRLDNFSDYQEGDTLEFYEIDKIAQAL"
     gene            complement(15513..16772)
                     /gene="nusA"
                     /locus_tag="PKK36_00945"
     CDS             complement(15513..16772)
                     /gene="nusA"
                     /locus_tag="PKK36_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNR93157.1"
                     /translation="MNNDLMAVFDYMERERGIDRETLIQAVEAALLNASKKSVGPHRD
                     MRVEIDRKTCNIRALAKVQVVEKVVNPHEEVSLVRARRVKADAQIGDQIEIEVTPKNF
                     GRIAAQTAKQAILHKIRQAEKDQVFDEYKDRQGEIVTGAVRRFDRNDVVVDLGRAEAL
                     MPSRERVPTEEYQIGDRIRAYLLKVDPQGPEVILSRSHPDFVRKLFELEVAEINDGTV
                     EIKGIAREAGFRSKIAVISHDDKVDPVGACVGMRGIRVKNIVRELSGEKIDIVKWHDD
                     IRTYVTNSLAPAKLSSVSVDEDTHTVSVVVDGDQLSLAIGKKGQNARLTSKLTGWKID
                     IRKHEAEISFEQKVNMAIQALAAVEGIGARYAEKLVLAGFLTLEGLLAADAEDLTAIE
                     GISEDAAKKLQAAAEAAFEKEHGPIEE"
     gene            17101..17742
                     /locus_tag="PKK36_00950"
     CDS             17101..17742
                     /locus_tag="PKK36_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93158.1"
                     /translation="MLRSRKILLMCGVAAAGFCLSATLAQGALVNLQAYIVFSLFRSD
                     GTTALPDGSIVQILGSTDGSIDPMQEYGTTGDYFAQPTGDDIIIGTIVVDSTILGIDG
                     TFYTGGFYYDNTVVDYMYIRFFDTTNSPIVGNYDWGQSPIFDVDGLDDGVMMFMDFGG
                     SYTASSNDTFVAIPEPSTASLLLLFAGLLCGVRAGMMRGPEEKKISGTGEIED"
     gene            18256..18642
                     /locus_tag="PKK36_00955"
                     /pseudo
     CDS             18256..18642
                     /locus_tag="PKK36_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006033033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18719..30934
                     /locus_tag="PKK36_00960"
     CDS             18719..30934
                     /locus_tag="PKK36_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNR93159.1"
                     /translation="MVGTFSSKTSNGWKFFFAALAALMPNMGFAWGWAVRDTARQSSE
                     NPTIYLGDSIEFHWDVNADGWGATYKKAGAGTNNSSSGMNWQDLTWVDEAGDGYGGNE
                     GIKSASFQGTSVATWYYSLWLGWGGSIGDNGHWYNGSSSWNEGSGSFVSSSFTVSALG
                     NPTGVSAARDATYPTSRVDLSWTEWNNRNVLIVRRKGSSVAWTPTQGTAYSDGQSLGS
                     DTYVVENSRDGGSFEDTGIGTALEAGTLYYYKVYSENYSYYSSGTEVSVRTRPNDATW
                     DGGGADNNTLTEANWNNDLYPAASANSVMRFAGSTRTTPSVTHAANSDFGSIYFNSGA
                     SAFTLSGNAVDIFSLIQNDSANAQTIQNALALNGTVSLSTVSGNLNLNGVLSGSYGIK
                     KTGTATAAITNANTFTGGAWVEKGTLQLGGHTNAMGSGAVSVGTNATLDLTYGSATLR
                     PVALNLYNGQVTKATSSATTWRGALNSYNSSTVSVSSGNFYVYGGMNLASGTLVFNNA
                     NQGGMSGGTMTGSGHLTKLGSGVFQLRPGTHSGNIALSEGEIRQYTGTMTASGTLTLS
                     GGTRYSSDGSTTRTITKDVVINGGVGIAENSTGGITISGAVGLGGGTRTITNSNTLTV
                     SGVVSDGGLEKKDAGTMILSGNNSYASGTTITEGTLQIGDNGTSGSVVGNIVNNSALV
                     FNRSDNLTYGGGISGTGTLDKQGAGALTLTNTSTYSGNTTITAGTLIQNGTNSSSAVA
                     VSSGTSLVGGGGVGGLTVTGSVYPGTSAAAIGRLTVTSLTMNDGSAAYFQIGDCDNTS
                     DRDYISNSGSATINATTTIYIDDDEVSNWDNAGSYSWNLITGGILDASNFTLDESLWA
                     LDKAGGSFALSASGGNLVLTFTPSAVPVAEINVLGTNSVSITDGDTTPALADGTDYGL
                     VLVAGGTKDHTFKIENTGTADLTVSGVTTTGTHAADYTVISWPGIVNPSASSNLIVRF
                     DPSSPGPRTATLHVNSDDDDEADYDFVIQGTGTYVEISVSGNSQYIADGDGSPTTDDH
                     TDFGPVGTIGATQDRIFTISNTGNVAMGIGNVTTSGTHEADFTVTEQPSSPLAPSNTT
                     TFTVRFDPSATGLRSAALSFTNTDDNVSDGLAESPFNFSIQGTGVAPAITNYPGTLSF
                     SSVLGSAPAVQSFSVTNIGLGSMSYSLTTNASWLTLKPVSGTVAGGAGQVHTAVVAVA
                     AGQQPGVSNATITITAADATNSPRTIAVSWTISAIPDPSVATAAADGKTMIDLAWTKH
                     ASYDVMIVYRAGSASTAPSQGTSYSVGSACGSGTVIYKGSGSALEHVVASGVTHHYAF
                     YSYNGNYYSSGLTDSDTTTAFAAGEIVETFSYTSGVTLASCNGSNLWGGAWYGDTGLF
                     TNSAGSFSEQANYPAPSGNKVLVTPANDANVAVYRALDRTYNDGRIYFGYVMNYQYEG
                     GDKYSGLSLYWNNTEEKLFIGEIGAADRQLGIDSTGSSVTLTNGAGNDYIIVGYYDWS
                     AGEARAKAYRIGSHTVPTDEPSSWDVTVSKASNSVGWVNSIRLAAGEFGGGSGTPGDC
                     YFDEIRISTNWAGLLQAAPSTPDDPSSQSATADGYEMVRLGWTKNGSGNSVLILHDDS
                     AIATDPTDGNSYSVGDSIGAAKVIYKGSDTAAEHIVEDGSDNYYKFYSYNGANYYSDG
                     VTADVSMGSYASYENVNPFSYTNNTAFGTSMKGGQGFGANYWAANSGTWKAQTNTGTA
                     TADTPKFFDLSGYPDMAGNLAWVENPGDGGSATADRDIDPAINSGTFYVAFMMSYQYY
                     GSNKWAGLSLMNSGTEKAFFGKGSGGNWSTLAAVAEGTTYWSAYDLAAFAGGGGNTGN
                     VYLVVGRYDFGTKEIKTKAWRVLDSEFPDTEPESWDASGTLSTGIDAINRIRLNVGSS
                     SAGAGTIGRVFFDEIRYATNWAGLIAVTCPTWAGSNTLGSTSTWLGDTESFEFQSYPV
                     GLGQTAGIEFDWSRDGSFSSYYDTFWWKNENSNSYWTNQVQMISAGVVTSRFVASGSG
                     CTPARTNNQPITVQNLNPPTDAAAVQDGTFSNSQINLTWTRGVSGGAKDTLVVRQTAD
                     SGWTVPVNGTTYNANDSLGSGTVVYRGALEEFDDTGLSPDTTYYYRFYAENWTYYSTN
                     YDEASAATAGGSQELVIDGGAADWLGTPATVYNASAGSLGEFIWTDKKGELRVDSGDH
                     PNGDISEFRVFADEDWVYFLVKMTNITDEAKPYVVIGVDTRRNSASAGMNWLGDDSGL
                     FAGGDYFGGGAAAVHYAEYQLNIHSAGGGAKIEMYAKDGAYWYAPPTGGDTNVAISTS
                     GNAVELKVARADLGLTGDVTGRFTVASFLNTGAWNNDGDGTVHIADNTADAIDSMSIA
                     PYNTADNSASLSAWLEDISDADIDFWLDVKFSGTGISANSKASTPVLVSPTNTAATSA
                     SPQLVWNKSTDSDGEVTGYLLEISTNEQFNGVTGTENGTIQLRVNLDANTTNYTFTTS
                     ASQYWWRVRARDTAGELSSAATRSFRVVGKLDTEGPLPTLLYIGTNVTGYLAGDYDTH
                     IATYGYIQSVLDSEIRDTNNVFGFVIQWSDASGVYATNQMKAADSPPNGAGGFAFNIV
                     DTDGRVSPNWDLVEIDTVNGTTNDLWGVDRPFYTSNTLAAGNGDLKVTNYVPAAFTVT
                     NYDPSIAYYLTLSAEDSYTEGGSWWDYGSWPSFASSAALPYYSGWCEDGPNTMRNITT
                     NYLIEVHVTDDDIIAPVASTDLAWENDASMTVSNSQGALAYVGGSGQDVLYQITDGSL
                     IGDWLTFSFNAYESYYSGIALGTSETFSHLGRTLTNTAFVAAYWQTNWANYSSSRSQT
                     SDTLSADTMITWYWPTITTQDVTKLWGPDSLSGEMGVTNIIQLDLYDTDNDRVEDQAS
                     ARVNFGRVRLVDDDAAPPGLSLTSIGGLSAQNFYTESGEMMYYDFGTGDAADLQPARI
                     FTSLSVADLTVLGVTPDTAAGESGNAVSYGYWNTATSYWEFAVTVQDGFRLTVQTIKF
                     GSRATSTGPTDWALKYSGDGYASDLASGSLNNNGSFETISASLTSVTNTGAVTFRLYG
                     SGASSYAGTWRLDNLSYTGKIATVSGTYLATDGDLHTNGLVIKASAQDSYSGLYAITS
                     PDGPSYDLISPAEASHNNAFGAGPAVDGSPTNQAVTLADTNGYPYADIVLGTYTAVVQ
                     VMDYDVDRDGDGLTVAVTGVVTVVDDDTGAPKYSTARGTYVFFDGVSAGSEGNAVTDG
                     MLTNGLSITNRLYDEKSGILSTSVQFRLLDPIGWDSGLQDFTTGPADGESLTNKFADD
                     TVVSVDGFDVDLQNRSLGVWTTLFYATDFDLDRPGDVLSTTQSLVMYVVDDDKLGPRM
                     TNFLSMGSAGVLIASGFEVIDGWEAHSEGNWTEEAFDGTWTGSNMYVNTLNGRGPEGT
                     TAGYNAGFNDVNDSLQMPPVDGPGWVVLWAKLSAEGESKMMLEWLDDSTWTSAGELSI
                     TSTGYFEYAWTVGMTNAGVSLRLRMTEKTTGNRSIYIDDIAVVPVRPWTNSAVSLAWE
                     ASSDADTGDSGIDEYRVVGLNAGAPLYTTNGAGVSALSTSVAPAVEGVVTGYVFAVDA
                     DLDRGSIDRTMGLAVPYILKIDTTPPAQIVVTNASEGPDDTSEIRIGWNPAADAGNPA
                     LSPWKSYVVFYTDDGGTADTNDMFIYVDSGGPSVLGDIGTREVILSNFVNGSTYNLAV
                     AGLDEAGNMGPLGGFTNVTLAVLSVTQSLAEVDTVVTNGVHVSFTATNSQGIMNRKID
                     VLYADSLQFVEALTNEWTLLGLADSTSFLIDTGQVSGVPPRQLGANMMRFYRAASQNN
                     WLPARNPRSASVEIFGVKPVTLYPGQNWISLPFWPTRQNILHVLGRSLPAGTTGGDEQ
                     ATRVSWYDRNNGGWLATQEVALIQSSPTSFWLYTEGGGGGDGNDAILPLHEAFVIELP
                     TNAAPQTILLTGRVPTNSMVQTVRAMHNSVPGRNLVSFNAPRYLHPSQMGLLEAGFQG
                     GAAPIWSDQIWKYDRSGQFAPYPVWFRTSDNTWRHTHPFPFPPVASNYFGPDDGIFIH
                     TRASTSDWSWTNKILYKLPTSRMDL"
     gene            complement(31029..31919)
                     /locus_tag="PKK36_00965"
     CDS             complement(31029..31919)
                     /locus_tag="PKK36_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNR93160.1"
                     /translation="MKPSLLILSAGIGSRYGGLKQLDPVGPSGETILDYSIFDAVRAG
                     FGRVVFVIRREMEQAFRERIGLKPVAADFAFQEMENIPAPFKPPAERAKPWGTAHAIL
                     SAADQLHGPFAVINADDFYGAEAFRALARHLSGDAETDSCMVGFILRQTLSPHGAVSR
                     GLCQTSAEGFLTGTSEHTDIRETGGGISGISPLGERIKLTGNEIVSMNCWGFMPGFLE
                     QTRMHFTDFLKEHIANPRAEFGIPAAVTGAIRAGAMRVKVLHTPATWFGVTYREDRPF
                     VQAQIRTLTDSGIYPEKLWA"
     gene            complement(31923..32783)
                     /locus_tag="PKK36_00970"
     CDS             complement(31923..32783)
                     /locus_tag="PKK36_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNR93161.1"
                     /translation="MIKLNNPGATLFFPDDFTPEEAFAHTTHLGIGAHQDDIEIMAYH
                     GILQCLHKKELWFGGIACTDGRGTPGNGPYAHCTEEEMHAIRAREQNTAAAIGQYSFV
                     AQLDYNSESIRNPVSEDLKNDLKQLLKTTSPSIIYTHNPADKHDTHVAVCVAVIEALR
                     ESPVGDVPPTVYGCEVWRSLDWLLDADKVIMSVAGHENIANALLGAHDSQISSGKKYN
                     RATVGRWLSNATFFDPRSQDIASKVSFAMDLSPLVQNPDLDISDYVQRYIERMAADIT
                     ARLDRQLKRS"
     gene            complement(32852..33208)
                     /gene="rplT"
                     /locus_tag="PKK36_00975"
     CDS             complement(32852..33208)
                     /gene="rplT"
                     /locus_tag="PKK36_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNR93162.1"
                     /translation="MPRATNAPASRKRRKRRLKLARGFMGGRSKLFRTATETVDRSMA
                     MSFEHRRRKKSDYRSLWVVRISAACKAHGITYSRLMDGLKKADIRLNRKMLSEIAIHD
                     PEGFGSIAAAAKAKLS"
     gene            complement(33225..33419)
                     /gene="rpmI"
                     /locus_tag="PKK36_00980"
     CDS             complement(33225..33419)
                     /gene="rpmI"
                     /locus_tag="PKK36_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNR93163.1"
                     /translation="MPKQKTRKAIAKRFKKTGTGKFKRSSAGGAHILTGKSRKQKRSM
                     ASAAMVSKADHKRIATLLHS"
     gene            33602..34447
                     /locus_tag="PKK36_00985"
     CDS             33602..34447
                     /locus_tag="PKK36_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR93164.1"
                     /translation="MRLVLGGVRGTSPVADPKFAEFGGDTSAFLVEGSNGEAIVIDAG
                     SGVRVLDPYLRQTDHSSAVLLLMTHYHLDHLMGFPSFSVIYDSTWDLEIKGPRLDGVA
                     ASDAVRHILSRPFWPLQLEALGGNIVFTDLPEDGGAPHIYHGLEVRWCPVPHLGGCVS
                     YRLDDPATRRSVVIATDVEWPLASETERHNFMEFCKYPAPPDLLVFDGQFTPDNYARF
                     RGWGHSTWQDAIDVAKLVKAGELIITHHHPRSDDEKLRQIEAEVKRQMPNAWLGRQDA
                     AIEIG"
     gene            complement(34453..35409)
                     /locus_tag="PKK36_00990"
     CDS             complement(34453..35409)
                     /locus_tag="PKK36_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNR93165.1"
                     /translation="MTKEKKLCIAGENQHSLCEAVAELLPLYSVDGKDSPEAVLSSYP
                     NATNIIEALKIYIDVMFPGKMTPDTIERGELGIFLLRRLSHGWRLLRPELEKAIPFRW
                     LGQAARTEGVEKVCDPAREAVNILRKLQKRLPDVRKLLVEDVRAAYEGDPAALTYAEV
                     QLAYPGLLAIASHRLAHELYRMKVPIVPRIMSEYIHHQTGVDIHPGATIGHGFFMDHA
                     TGIVIGETTTIGNHVKIYQGVTLGARSFDLDEQGQPVKHIKRHPTVEDNVVIYANATI
                     LGGKTVIGRNSTIGGNVFLMETVPPNSFVASEHPELRIKQSK"
     gene            35488..36201
                     /locus_tag="PKK36_00995"
     CDS             35488..36201
                     /locus_tag="PKK36_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93166.1"
                     /translation="MRITSVLIMKRFVKIALIAAAVLVLIVPLFSRILARKAVVWAVG
                     RETGLNLEIGRLKIGKLGSWFEITDFRLMNPEGFEPGPCVEIARLHVRYSPRSLFSDT
                     LRISRLYLDISRVLIVEEGGASNLDPLVEKTLSAPVRPETARSAHKSRPAQTEPDSSP
                     PPTTSDGRKDVSIGELTLKLGTLELRRRAADEDEPVIQEFVFNETVVLTNVVSPEQVV
                     QRVAAGFLVKQMASGLLEP"
     gene            36276..36352
                     /locus_tag="PKK36_01000"
     tRNA            36276..36352
                     /locus_tag="PKK36_01000"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36310..36312,aa:Pro,seq:ggg)
BASE COUNT         7413 a        10380 c        10921 g         7643 t
ORIGIN      
        1 tccacgggcc cgaacacgct ggtgacctgc tggggcgcgg tgattattct ttccggcgca
       61 cggaagcctt tgagttcatt gcacagcgct tcaatatgcg cgggggttgt gccgcagcat
      121 ccgcccgcca cgttcaggcc gacctcgcgg gccatgcttc caagcttctt tgcgaattcc
      181 agagcctgca aaggataatg gacctgcccg ctgcgcagtt ccggcatgcc cgcattgggc
      241 atgcaggcca gaagccccgt ccagttttcg gccagatagt cgaggtgaat tcgcatggct
      301 tcgggtccgg ttgcgcagtt cagcccgaga atgttgacgg ggagggctga gagcgcggcg
      361 actacggccg cgatgctgga accgatgaga agtgtgccgg tctgttcaac cgtcaccgag
      421 acatagaggg gcgctccggc cttgagcccg ccggcctttt caaacgcggc gatgccggct
      481 ttgatctgga gaaggtcctg gcaggtctcg aacagaatgc cgtccgcgcc gccttcaagc
      541 agtccttcga tttgcacagt caggccgtcg cgcaactggt cgtacggaat ttgtccaagg
      601 gttggaagtt ttgttcccgg cccgacggaa ccgaaaacgt atcgcggccg gtcgggggtg
      661 gaggcggccg ccgcggcctc gcgggctatg cgcgcggcgg cgaggctgat ctcgcgggct
      721 tttgcggaaa gatcatattc gcccagtgta acgggcgagg aaccaaaact gttggtctcg
      781 acggcatcgc taccggcggc gaaataggtg gtatgaatct ggcggatgat gtcgggtgcg
      841 gtcaggttca gcagttcgtt gcagccctct ttgtcctgcc atgcggacgg aggcacgtcg
      901 aaattctgga tctgggtgcc catggccccg tccataataa aaatgccttt tttgattcga
      961 tccagaagtt tcatgctcat gcgcctttct gcaaatgcga agagcaccat agcacagaaa
     1021 atctcgtgga ggttacaaaa aaagcccgct tcaggaagaa agccccgcat tttattcgag
     1081 taaaatcttg ccgtcgccgc ggtgcgggag taggcttatt aacgtgtcag gaggagatat
     1141 gaaaaagttc caatccctgg aaaacctcga gaaaaaagtt ccaaccattg gaagtttctc
     1201 gctgaaaagt tccaatcatt ggaacttttt acgcggcctt cttttcggcg ctggaatgat
     1261 gatgaccggc accagtcccg ccgccgggcc gtctcctggc gtctcataat gagacaattg
     1321 tcttaatatg agacgattcc gccctccatt gaatattcat aattatctaa taatgaatat
     1381 ataacagcat tctaccaatc ttggcatgcc atttgcatta gatgggtgca ggtggggatg
     1441 ttgagatgaa ggcacaaagc tatagaatgc agatgaccgg cagccgaatc cgcgggggat
     1501 accgtgcggt tttggttctg gctctgaata ttcttacttt gtgctttctt tccagcacat
     1561 ccgccgccga gggccgcgcg ccgggctggt tccgtcgtca cttcggggat gtccgctggg
     1621 aagaagcggt tcagaacagc cgctcgccca aagacgtttg cggtttcact gccgaccgcc
     1681 tgcgctacag cgcggaaagc ggcgacgtct ggagtgatcc ggccgagatt tggaatcgcc
     1741 gctccggcga ttgcgaggat tacgcggcgg tggtcatgga tatgtgccgg gaagccggtt
     1801 tcgaggcgtg ggtggaagtc tatttcccgc gcggccgcgg cgaaggccat gcggtggccg
     1861 tggggcctct tcccgaaggg cgatactggg tttccagctt tgggaaatac gaaacggttt
     1921 cctcccgtga agaaatctcc cggctgatgg ctggcattct ctggtgtgat gccggggatc
     1981 tgtggagcgc ccgcatgagc tatgaatcgg tgcatgggaa aatcgcccgc cagcagcagg
     2041 cccggctcag ccgcatggcc gcagccgcct cttctccctt ctgacggccc gtcagttcag
     2101 cagtttatcc acggtctggt acagttcctg aacggatatc ggcttgcaga ggaagccgtc
     2161 tgcgcttgtc tgctcctgcc agtaggagtt gtctcgcccg gcatttgagg ccaccccggt
     2221 gatcatcagg atcgggatgt tgcggtattt ctccttgcgt ttcagttcca tgcagatgtc
     2281 gagcccgttt ttgcggggaa gcatcacatc cagaatggcc agatcgggac acatgttctt
     2341 gatggcttct tcaccgtcct gcccgttgtc atagcagtaa acctcgtagc cccgttcctc
     2401 cagcgccatt ttcaggaaac ggctgaagtt gcgttcgtcc tccagaacaa caatcaaacc
     2461 ctcgctcatg tgggagaaga tagtcatttc tgtttcattt gttaaaccca aaagaggaac
     2521 atttgacatt gcgcccggcc ggaacctctg atagcctttc cgtttcggcg tcaaagaaat
     2581 ttggacgccg ttctgtttga gaagaggtgt gatatggcgg gcatggacat tttctttctg
     2641 gtcttttctg ttctgggcgg gctggcgctg ttcatctttg gaatgaacgt gatgaccgaa
     2701 gggctgcgga ctgcggccgg cagtcagctg cgggtgattc tggccaagac cacaggcaac
     2761 cggatgctgg gcatcgctct tgggacgggt ctgggtttcc tggttcaaag cagcgcggcc
     2821 acggtgatgc tggtcggctt tatcaatgcc ggcttgatgt ctctgcttca gtccattccg
     2881 gccatgctgg gcgccaacat tgggacaacc atctccatgc agcttatttc attcaagctg
     2941 ggcgcatact gctatttcgc catcacgctg ggattcatca tcaacatggt tgcgcgaggg
     3001 gtgaaagcca gatccatggg ccgcgctctt cttggattcg gactgctttt cctgggaatg
     3061 aatgtcatga gcgatgccat ccgtccccat cgcgaaaccc tggctcctct tctgctgagc
     3121 gctgacggcg ccacgataaa gggaatgctc ataggggttc tgttttcaac cgcttttacc
     3181 ggaatcatcc agagcagcgg agccaccatc gggatggtct tcgccatgat ttcggcgggg
     3241 gttataacga gcttccagca ggcctatccc attgtccttg gcgccaatgt cggcacttgc
     3301 gccaccgcgc ttctcggcag catcggtaca aacatcgaag cgcggcgttc tgcggtctct
     3361 catcttctgt tcaatatttt cagcgccgtt gttgggattc tcacggctcc tcttttctat
     3421 cgatatattc atcttacatc gggagatctg atccatcagg cagccaatgc aaacaccatc
     3481 aagatggtgg caaccgctct tatggtgctt ccctttgcgg gtatccacgc ccgcattgtg
     3541 cgggcactga cgccatcgcg cacccccgcg ccggagaaca gtttcctcga cagcgagctg
     3601 attcccacgc ctgaaacggc tatttccgcc gctgttcagg agctgcagcg cattgcccgc
     3661 gtctgcgcgc gcagcatgcg cctgaatgcc gatctgctgt ttgacccctc caagaagatc
     3721 ctgcaggcga tcaagctgaa cgagggcgtg atcaacgaga tcaagacggc gatgaaagac
     3781 tatctgacga agctcggtca ccgtcagctt tccgagcggc aagccatcat cattcttcat
     3841 ctcgaccggt gcatgacgga tattgagcgc ataggcgatc atattgagca gattgcggat
     3901 atttcgtggg cccgccgcca gaaaaagagc gaggcgattt ttgatgaaca gagtttttcg
     3961 gagatgttcg ctctgtatga aaaggcgctg caggtgctgt gcctggtgat tgagtcgctg
     4021 gatatcaagc agggggattt ccaggacatt gccaagcgga tactggccat gcgcgatgaa
     4081 tacatcacgc tgaacatcga gacacgctct cgcttcattg gcaaaatcgc gcggcaggaa
     4141 atgcccccgc tctgcgggat gtattacgcg ggatatctgt cggcatttga gcggatcgtg
     4201 aagcactcca agtccattgc tctggccgag cagcagccgc agtttctgat caagcggggc
     4261 aagctcggtc aagtggcgcc gcaggctccg gactacaagc cgccggagct tgtggatccg
     4321 aatgatttcc tggacagact tcagagtgaa cggtacatgt agaccagaaa cgggggaatc
     4381 aatgaaatct gcaaagcgta tattcatcat ccttgtgatg gtccttgcgg ccattctgct
     4441 tgcggccatg ctgttcctcg ggccgctcat caagacggca gtggagaagg gcgcgccggc
     4501 catgatgggg gtgcctgtca cgctggatcg tccgggcata aatctgctgg ccgggaaaat
     4561 cagtctcaac aacctcgtgg tcggcaatcc cgaaggctac aagaccgatt atgcctttaa
     4621 actcgggaag ttccgcatcg acatgcagat gagcacgctg ggctctgaca cgatgattat
     4681 cagggatatc ctgattgaca gtccggaaat tgtctgggag gccggtctgg gcagcagcaa
     4741 tatcggcacg atactcgatc atctcaaaaa gaaggccccg gcggaaaaac cggaagcgga
     4801 aaaggaagag ccgaaaaagg ccggcacgca ggtgcagatt gacaacctgc tgattaccgg
     4861 cgccaagctc aagttcagcg ccaaaatgat ggggggacag gcgatcacca ttccgctgcc
     4921 cgatatccac ctgaaggaac tgggaaaaga caaggacggg gcctcgattc tggatgtgat
     4981 caccgaagtg atgaacgcgg tggtgtccgc gatcggcgag gccctttcct ccgcgcccga
     5041 gctgcttggt aaaggttttg gcgccgcgat gggtgtggcg ggcgacggcg tgacggccgc
     5101 cggatcgctg gctgacgacg gggtgcgggc agttggcgga attgccgccg acggagccaa
     5161 ggcggccggc ggcatcgtca ccgaaggcgt caaggccgtc ggcgagggcg caggcgccat
     5221 tatcaagggc gtgggcggtc tgcttcccgg cggggacaag aagaaataag cctagaaccc
     5281 gaaagactgc tgcgcagccg gcgcgatgca gtccgggtga tcatggcgcg cattgttcac
     5341 ttttgcgctt acgggatgca tgtgcatttc gtttgcgctg aacggctgca gcatctgctc
     5401 gacttccctg tccgggatca ggttctgcga gatccactgc tcaaaatact ccggcttgag
     5461 aatcaccggc atccggtcgt gaacggtttc cagcaaccgg ttcggccgcg tggtgatgat
     5521 ggcgctgctg atgatcaggc cgccttcgcg gtcctgccat tcgtcccaga gcccggcgaa
     5581 agcaaagact ccgccgttcg ccatccggat ataaaccggc ggtccctttg aagcgctccg
     5641 gggccattcg tagaatccgt ccgcggggat caggcagcgt ttggtgcgca gacatgcgcg
     5701 gaagctcggc ttttcgctca atgtttcggc gcgcgcgttg accagcggtt tccattttcc
     5761 cggcccgccg gaccatccgg gaatcagtcc ccagtgcgtg aaggtcagtt tgcgcgaacc
     5821 gtcgttgagc agcgtgagca ccggctggga gggggcgatg ttgtagcggg gctcatatgg
     5881 actgaccggc gccgtccgca gcagttccgt caccgattca atctttgccg tcagtgtaaa
     5941 tcgtccgcac ataggccctg atcgaagcat agtccggccg gcgcgtcaat gacgccccgt
     6001 caccgggcct tccgcgggct acagcccgag gcgcggcaga gaaagcgcgc gcggggccgg
     6061 gatcagcttc tgatactccg gaaggcttcg cagcggctcc agccgcgggt cttcgcgcgc
     6121 gattttgcga atcggctcgc cgccaagctg aacggccctg gtcagtgcgc tgatggcttc
     6181 ttcctgccgt ccggccatgg ttttcaccgc ggcatagtcg aaccaggccc ggtcattgtt
     6241 cggctgcacg gcgagatatt tttcgagcgc aagcccgagc agatcaatgc ggccggcgtt
     6301 ggcgcagcgc tggccgattt ccatgtacac gttttcggga accccgggct gctccaggag
     6361 cctgcgcatc agctgctcga atgacccctg catgttgagc tggagataga catcgctgag
     6421 ttcaaaaatc gcgtcaagcg tggctccgcc gccggacagc tgcgcctcga gctgcatgcg
     6481 ccggttttcg gaggtttccg cgccgacaat ctggttcaga aacgactcca ccttgtcgtt
     6541 gtccttgtcg agcttcagga agttctggat gagttccttc gcctgggtga acttgcgctg
     6601 ctgcatatag agttccgcca ggcggaagtt ggcctcgggg ctgagcggat agagatcaat
     6661 ggcctgggtg aaggcctcct cggcctcggg catcatccgc cgggcggcat aaaggccggc
     6721 aatggcgctg cgcagcttgg agaaggtttt tcgggcgacc acgtcgcggc ggaatttcgg
     6781 gttgctcgtc agatattcct tgtaccatgc ccagaacttg cggtcgttct ccaccatttt
     6841 cggggtcagc cccggcagcg gctccggatt gatcttcatg atgagaccgt gcggttcaag
     6901 atagggatac atccacggga tgacgtagct ttcctcgaca tagaaaggat gcttgtactt
     6961 gttgtgctcg aagatcatct gggcaagaat gccgttgatc atcatcacgc cctgcacgcc
     7021 ctgcacgctg acgcgtccgt ccttgaagga cacctcggcc ccgggcggga tgcggccctc
     7081 ctgcacgtcg cgcacatact gctggaaggc gagattgctg tcctgctgcg agggaatcca
     7141 gatatcattg cccatcaggt cgcgcatgac gttcatgtag gtgttgtcgg ccagtgcgtt
     7201 ctgcgtgatc aggaaaacat cgcggcgcac cttggcggaa tagatcatgt aggtgggcac
     7261 gaaacgtccc ggatcggtgc cgccgaagaa gatggcgttg gtgcccattt cgggcggata
     7321 gccgggagtc ggataatccg cccattcctt ctcaaactcg cccggcgaaa gccagtgccg
     7381 gagatcatcc tcgattccgt tcacgccgcg aagctgccag ttgccgaact gccagccgaa
     7441 cgtgtggccg ttctgttcgc agccgccgat gatgcggaca aattccttgt cgtatgcgtt
     7501 tcggaggatc ggcacgagcg gaagcagcag cacgcagatg gtggcgggct gccgcaggag
     7561 ccatccggcc cgggattcca gaaagaccag agcgaacatg agcccgtagc tgatccagag
     7621 cgcgaagacg gcatgggcct gaatgtactg cacacgtccg atgaacagcg tctgcacgtc
     7681 gagctgcgga ttctggaaca caatgaagat caccgtcatt gaaaggaacg ccgtgagcgt
     7741 ggcccatatc catgcgcggg actggcgtgt gattttgcgc aggaaggcga agggcaccag
     7801 cacgaacagg gaaagtatcc atgtgaactg ggccacgacg gaattgtagc cgcccggatt
     7861 cagaatgatg gcccagagct gccggaaaaa gagcgtctgg ttctggccgt actgccgcag
     7921 agtggccatg gggctgatct tttcgtactg cccgcgcgtc agggcatgca tgaagccttc
     7981 ggtggtgcgc ggatagcccc agttcatcgg cgggttctgc tcggaggcga ccggcatgta
     8041 ggcgtagaag gaaactccga gggccagcag tccgaaaaca atgagcacgc gtttccattc
     8101 ggtgaagagg ctgcgctgca tgatcaggaa aaccagcgga gcgaacagga acacgaaccc
     8161 gacggcaatt ttgacctgct tgtcgcgcag ggcttcggca gagcctgcca cattgatctt
     8221 ggaggcggaa taccacagat agacggcgat gaggatgaag ccgagagaaa gcgcatcgcg
     8281 gaaaagccgg cgatccctga accagaggcc ggtcagcagc gccagcacga gaaacagaag
     8341 gctctggtgg ttggtgaggc cgaggccgaa gatgaaggcg gtggcgtaga gaattttgtc
     8401 gtcctgcggg cggcacatcc agcggtagat caggatgagc agcagcgcga gaaagaaggc
     8461 gttcaggctg tagacctcga cgatggtgga ctgcgaccac agcaccgagc tgaaggccag
     8521 caggaggccg ccggagatgc cgcagctcag gcagatggcg ctctcggtct cgcggccgag
     8581 aatgcgggtt tcctttgcca gtccgcggag gatgtcggct cccgaccggc tgaccagcag
     8641 cgcaatgatg gccgcggcca gcgccccgaa gaacgccgag gccaaaccca ccgcccaggc
     8701 ggggtttgga cttccattgt aggagtcaaa atggaagacc cactgaaaaa accacgtgac
     8761 cagcgtccag atgggatacc cgggcgggtg ggggacgccc agatagtcgg aggccacggc
     8821 cagctcgccg gagtcctcca gcgagaccgt gggcgacagt gtgtagacat acacgccaaa
     8881 agcgatggca aaagtgatga agaacgtcag ccagtccacc ttgcggaaaa aagccccgct
     8941 ttggtgatca aaaaagtcgt cccgcacttc ggcctgttcc gtattcgacg caatttcccg
     9001 agccataagc aatcctgtgt tgtcacccgt ttcagacagt tcctgtgccc gttttgttca
     9061 cagggaaact gtaaccgcgc cagtatggat gattcccgca ggccgtcaag cgcgcaggcg
     9121 gtcagatgtg caggataatc acggccagaa cggtcaggcc gtagaggagg atgttcacaa
     9181 tgagggggat gtccgtcagc aggatttttt cgggccgtcc gccctgttcg tgccggtaga
     9241 ccagatcaag atagcggaaa aggccgaaca tcacaaaggg aatggtgagg gccagtttgg
     9301 cggtgccgaa tttctcgatg gtgtccggcc agagggtgta aagagaatag aaaacaatgg
     9361 tggcggtgct gacgatggcg atcagctgat caagcaggcg ttcgttgtag gatccaaggc
     9421 tggggcgggt gtcgctgccg gtcgcatcaa tgatgacttt ttcatggcgg cgcttgcaga
     9481 gggcgaggaa aagcgccagc agcagcgtgc acagcagcag ccatggcgag aacacgacat
     9541 ccaccgtgac ggctccggcc acggcccgga gaacgaagcc ccctgcgatc acaaagagct
     9601 cgatcaggcc gatgtgcttg agccaaacgg tatagatgat ctggagaagc acatatccgg
     9661 cctcaacaag cgcaaggcgc ggacagatga agcccgcgcc ggccagtccg gcggccagcg
     9721 aggcaaaagc cagcagcaga gatcctttaa cgctgatttc accggcggcc agcggccgaa
     9781 atttttttgt gggatgaagg cggtcgcgtt cgacatcgcg aatatcgttc aaaaggtaaa
     9841 tcccgctgga tatgagacag aaggtcagca tggcgccgac ggcatctcca agctgaccca
     9901 gcgacacggc ctgatggcgg tctcccaggg cgaaaacaaa agcagccatc accacggcat
     9961 tcttggtcca ctggttgggg cgcagggctc gaaagtagga gaaggccgct ttcattgtgc
    10021 ctttcggggg ctgcggcgga agcggcagcg cgcagcaagt ttccagacca tccagagcgc
    10081 ctcgaaaaca atgcctgaac tcattttgga ctgaccttcg cgccgctctt caaaggtgat
    10141 cgggacctcc tgtatctgga agccctgcag ccatgcggtg tgcgtcattt caatctggaa
    10201 agagtatccg ttggagcgaa tggaatccag atctatggat tcaagcaccc tgcgccggaa
    10261 gcatttgaac ccccccgtgg ggtccatgaa aggcatcccg gtgatgaggc ggacataaag
    10321 gctggcgccc ttgctcagaa tcaggcggtt gaggggccag ttgatcaccc ggataccgcc
    10381 gatatagcgt gatccaagaa ccaggtccgc tccgctttcg gctgcctcgc gcagccgggg
    10441 cacatctgcc ggattgtgcg agaaatccgc gtccatctca aagatatact ggtagttgcg
    10501 ctcaagggcc cagtgaaagc cggcaatgta ggcgcgccca agaccctcct tgcgctcctt
    10561 gtgcagcaca tgaatccggg tgtcgctcga ggccatttca tcggcaatcc ggccggtgcc
    10621 gtcgggggaa ttgtcgtcca caaaaaggat atgtgcgccc ggcgaatggt tcagcgctgc
    10681 cgccgcgatc tgctggacgt tttccctttc attgtacgtc ggtatgacaa tgacagtgtc
    10741 gttcatgttg cgcgcattct aggggaaagg cggccatccg gcaagccgca gaatggggat
    10801 aatgggttga atctgcttcg ggttgggtta tagtggctgc tgccatggaa ctgaaaatca
    10861 aaacatatgg agatccggtg ctccgggaac gggcgcgccc ggtggatgaa atcacgccgg
    10921 aaatatgcga cctcgcggag gaaatgctgc gtgtgatgca tcagcagaac ggagtgggtc
    10981 tggccgcgga gcaggtcggc cgccgcgagg cggtggtggt gattgatatt ccgcccggcg
    11041 cggactgcga caaggaaaaa aatccgctga atccggatgc cgtcatgccg ctggttttga
    11101 tcaatcccga aataacagaa agctcaaaga agaaggaatc atgcgaagaa ggctgtttga
    11161 gttttccggg cctttgggcc gaggtgtccc ggccggcaga ggtgaccgtc cactataccg
    11221 gtattgacgg ccgcccgcag gacatgcgct gccgcgggct gctggcgcgg gccgtgcagc
    11281 acgaactgga ccacctgaac ggaacgctgt tcatagaccg catatcctat gcccgaaaaa
    11341 ttgccctgaa aggccgcctg aagaagcttc aggaagaaac gctggagtca catctgctct
    11401 gacaggatgg attttgctcg ccggacatcg tcttcaatct gccgggtcag ttcggcgggc
    11461 gtgtcgaatt tcctgtcgct gcggattcgt tcagcaaaac gaacgatgat ttccctgtca
    11521 tagatgttct cccgcagatc aaaaaagaac acctccagcg accattcgtt ccccgcctgg
    11581 gtaaaagtgg gccgccggcc gatatagcct gcgccgtgaa atatcccttc gccgatttca
    11641 gcggtgacgg cgtagatacc ctccggcggg aagacctcgt tatggggccg gatgttggcc
    11701 gtgggaaaac ccatggtgcg gcctatgccg cggccccgca cgaccgtgcc ccatatgctg
    11761 aagggctgtc ccagcatggc ggaagcgtcc ttcagatttc ccccggccac ggctttccga
    11821 atgcgtgtgc tggaaaccgg ttcgccgtca cacgtcacgg gggccaccgt ggatgttccg
    11881 atgccgtgcg caagggccca ctgtttcagc acgtctgctg tgcccgttcc cttgtggccg
    11941 aaggtccagt tggatccgac aacaatatgt cgcagcgtgg gaatggcctc cgtcagcatt
    12001 tccaggaact ctgatgccgt cagatggcga aacgccgggg cgaatggcat caggatgcat
    12061 ccctgcacat tcatctgttc gaagatatgc agcttgtgcc gtgttgccgt cagcagcgcc
    12121 ggcgccgttt cgggggagat gactttgcgc ggatggggtt cgaaagtcag gatccagagt
    12181 tccgccgcaa gtttttccgc ctgcgcgcgc gcggcttcga ttactgcccg gtggccgaga
    12241 tgaactccgt caaaaaagcc aatggccagc accaccgggg cggtctcggt cttcagggtg
    12301 tcgagatggc ggaccacttt cagcatagaa tctgctcgtc ctgcttcacc tgaagaaacg
    12361 ggatgaccag cgattccagg tctgcacggg agatctgcat gaggtcgtcc agggtccggg
    12421 cgcgctccac ccgatagctt ccgctttgtg tccggcgcag cgcctccagg caggcgccgc
    12481 atcccagcgc ctccccgatg tcatggcaga gtgtccgcac ataggtgccc ttggtgcact
    12541 tgattcggaa ctgcgcccgc ggcgggctga aatccagcag gctgaaattg aagacatgga
    12601 tcagccgggg ctcgcgctcg accgtgacgc ccttccgggc cagcttgtag agcggcacac
    12661 cgttcttctt gatggcggaa accatcggtg gcgtctgcat gatgtcgccg gtgagcttct
    12721 gcatttccgc ggcgatctgt tccgcggtga tggcggagca gtcggcttcg cgggtcactt
    12781 cgccgtccat gtcctggctg tccgtggtga ttccgaggcg catgatgcct tcataggtct
    12841 tgttggaact catcacctgt gaggaaagcc tggtgccgcg gcccagcaga atcagcagca
    12901 ggccggtggc cgcgggatcc agcgttccac cgtggccgac ctttttgatg ccgaaatgcc
    12961 ggcggattat gtgaaccaca tcgtgggacg tgatgccggc gggcttgtcc accagcagca
    13021 gcccgtcgag gccgcctgta tcgggccggt tattcatcgc cgtcgggttt ttccgtgtcg
    13081 tcgtcactca ggccgagctc gctgatgatg gacaatatgc ggtcgccttt ttcgatggat
    13141 tcgtccagtt caaatgtgag ctgtggtgtg tacttcagct tcagatccga gttgatgtgg
    13201 cgctggatgg tgccccgatg gcgggtcagc aggtcaatga tgtgccggcg ctcctgctca
    13261 tgatcacgca cggataccat cacccgcgcg tgccggatgt ccggactggt gatcacatgg
    13321 gtcaccgtca ccgccgcgag atccagtttc tcctccccgc cgattttata gatgactccg
    13381 gcgatttcgc gcttcaggag ttcgttcact cgttcgagtc gtcttacgct catttgtcat
    13441 tctcccggga tggggcgccg aaaacaggaa cggcagggcc taaagcgcct gcgcgatctt
    13501 gtcgatttcg tagaattcaa gcgtgtcgcc ttcctggtaa tccgagaaat tgtccaggcg
    13561 gatgccgcag tcctgtcctt cacgcacttc ggcggcgtcg ttctgaaagc gcttcagaga
    13621 tgccaggctg ccttcgaaga cgacatcgtt tttgcgcagc acgcgcacct tggcgcgcga
    13681 agtggcgcgc ccgtcggtca ccatgcagcc ggccaccttg ccccgcttgc tcagtgaaaa
    13741 cacctgccgg acttcggcgc gcccgatcac gcgctgcttg aattccggtt cgagcatgcc
    13801 cgtcatggct tccttgatct gatcgaacag ctcatagatc acgctgtaga gacggatttc
    13861 aacgccgctg cgcttggccg ccgccttggc gccgccctcg gcgctcacat gaaatccgac
    13921 aacgatggca ttggatgcgg ttgccagcag aatgtcgttt tccgtgatgt tcccgacccc
    13981 cgtcaggacg agattcaggc tgaccttgtc gcttttaatt ccctgcagca tctgctcgat
    14041 ggcctcaacg gatcctttta catccgcctt gagcaccaga ttcagcgtct gctgcgcatt
    14101 ctcggatatc tggctgaaaa gattgtccag cgaggccctc tggggcgccg tcaggttttc
    14161 catccggttc tgttcctggc gtgattcggc aatggagcgc gcttcgcggt cgcttgcgca
    14221 ggcaataaat tcggagccgg catcaggcac actcgtgaga cccatcacct ttacgggcgc
    14281 ggcgggtccg gctgtgcgga ctttatgccc cttgtcgttc atcagggcct tgacgcggcc
    14341 ccaggttgat ccgcatacca cggcatcgcc gactttgagt gttccgtcgc tcaccagaaa
    14401 tgtggcggtt ggccccatgc cgggttccag acgcgcctca atgacaaaac ccctggcgga
    14461 ctgccttgga ttggccttga gttccagcat gtcggcctgc agcagaatca tttcaagcag
    14521 gtgatccatg ccctgcccgg tatgggcgct cacggggcag cagatgatgt cgccgcccca
    14581 gtcttcgggg gtgagaccca tctcctggag ctgctgcttg acccggtcgg gattggagcc
    14641 cggaagatca gacttgttga tggcaacgat aatggccact tccgccgcct gcgcgtgctg
    14701 aatcgcctcg cgggtctgcg gcatcacgcc gtcatcccct gcgatcacca aaaccgcgat
    14761 gtcggtcaga ttggcgccgc gcgcgcgcat cgcggtgaat gccgcatggc cgggcgtgtc
    14821 cagaaatgtg atcgggcggt ccatgtgctc gaccatgtag gcgccgatgt gctgggtgat
    14881 tccgccgtct tcgccgtcca ccacagaact cttgcggatg taatccagaa gcgaggtttt
    14941 gccatggtcc acatgcccca gaaaggtcac tacaggcggc cgcagttctt cgccgccctt
    15001 tgcctcttcc tcctccttct tctctttctt cttcgagggc ggggggggcg gcggaggagg
    15061 agggggcgcg gctttttcct gctcgacctt cacgccgtgc ttctcgccca ccttctgggc
    15121 caccttgaaa tcgatcgtgg cattgatggc ggcaaagatg ttcatcatca tcagctctgc
    15181 gatgagctga ttgggcttaa gcttcagaac atctgcaaac tccttgacga tgatggggcc
    15241 cttcacgatg taaacatttt cggccggggg ctcaaccttc tctggttccg gtgtcgcggg
    15301 cggggctggc tcgggctgct cgggcgcggc agcgggtttt tccggagcgg cctccggctt
    15361 cgctggcttg ccgccgacgg cttcgcggac ctgcgcaatc tgctcatcgg ccagggcgga
    15421 catgtggttg ggggcttcga tgcccatggc ccgaagcttg gccagcaggt ccttgctgct
    15481 gatgttcagt tcttttgcca gttcaaaaat tctcattctt caatgggtcc gtgttctttt
    15541 tcaaatgcgg cttctgcggc cgcctgcagt ttcttcgcag cgtcctcgct gatgccctcg
    15601 atcgcggtca gatcttccgc atctgcggca agcagccctt cgagcgtcag gaatccggcc
    15661 agcaccagtt tttctgcata gcgagcgccg atgccctcca cggccgccag cgcctgaatc
    15721 gccatgttca ccttctgttc aaacgagatc tccgcctcgt gctttcggat gtcgatcttc
    15781 cagccggtca gcttggatgt cagccgcgcg ttctggccct tcttcccgat ggccagcgaa
    15841 agctggtccc catccacaac cacgctgacg gtatgggtgt cttcatccac gctgacgctg
    15901 ctcaacttgg ccggggccag cgagttagtc acataggtgc ggatatcgtc atgccatttt
    15961 acgatgtcaa tcttctcccc ggaaagctcg cgcacgatgt tcttcacgcg aattccgcgc
    16021 atgccgacgc aggctccgac cggatccact ttgtcatcgt gggatataac cgcaatctta
    16081 ctgcggaaac ccgcctcgcg tgcaatgccc ttgatctcga ccgtgccgtc gttgatttcg
    16141 gccacttcga gctcaaagag cttgcggaca aagtccggat gactgcggga gagaatgact
    16201 tccggcccct gcggatccac cttgagaaga taagcccgga tgcggtcgcc gatctggtat
    16261 tcttctgttg gaacccgttc ccttgaaggc attaaggcct ccgcgcgacc caggtccacg
    16321 accacgtcgt tccggtcgaa ccggcggacg gctcccgtta cgatttctcc ctgccgatcc
    16381 ttgtattcat caaaaacctg gtctttttcg gcctgccgga ttttgtgaag aatggcctgc
    16441 ttggctgtct gcgcggcaat ccggccgaaa ttcttggggg tcacctcgat ttctatctgg
    16501 tcgccaatct gggcgtctgc tttgactcgg cgggccctga cgagagacac ttcttcgtgg
    16561 ggattgacca ctttttcaac aacctgcacc ttggccaggg cccggatatt gcaggttttc
    16621 cggtcaattt cgacccgcat gtcgcggtga ggccccacgc tcttcttgga ggcgttcaac
    16681 agggcggctt ccaccgcctg tatgagcgtt tcgcggtcaa ttccgcgctc tctctccata
    16741 taatcaaaaa ccgccatcaa gtcgttgttc atttttctgt cctcaacacg gactgtcggc
    16801 agcccgcggc taaaaacaaa accggcccat tatacaaaag agcgggcgaa ggcccactct
    16861 gtacaaaggt cagcgcaaca aaaaatacag ccagaacata atctttgtgc gcgaacaggt
    16921 caaggcgttt gttccgaaag atgcgcaatt attttttcac atttttttga cataatattc
    16981 aacttcccgt atatattaag taagtgcaga tagaaagagg caggcgttgc ggcatggatt
    17041 cgcatcggcc cgcttgtggt ttatggtgac agttatacct gtacaccacg ggagagagat
    17101 gtgctgagaa gccgaaagat attgctgatg tgcggggttg ccgcggcagg attctgtctg
    17161 tcggcgacgc ttgcgcaggg agccctggtt aatctgcagg cctatattgt tttttccctt
    17221 ttccggagcg acggaaccac cgcgctcccc gacggatcca tcgtgcagat actcgggagc
    17281 accgacggat caatcgatcc catgcaggaa tacggcacca ctggcgacta cttcgcccag
    17341 cctaccgggg atgacatcat tatcggaacc attgttgtgg acagcaccat tcttggcata
    17401 gacggcacgt tttataccgg cggattctac tacgacaata cggtggtgga ctatatgtat
    17461 atccgcttct ttgacaccac gaactctccg atcgtcggga attacgactg ggggcagtcg
    17521 cccattttcg atgtggacgg acttgatgac ggcgtcatga tgttcatgga tttcggcggg
    17581 tcatacacgg cgagcagcaa cgatacattt gttgccattc cggagccgag cacggcgagc
    17641 ctgcttctgc tcttcgcggg gttgctttgc ggcgtgcggg cgggaatgat gcgcgggccg
    17701 gaagaaaaga aaatttcggg aaccggggaa atcgaagact agggaataaa catgaggaca
    17761 gggtgcacag gcgcttgcag caaaaggtta agcgctttac tgcttggcct cgctgtcctg
    17821 tgcccgctcg ttgttctcgg tcagtccgga tctgtcacaa ctccccttca tgttaacgca
    17881 tcatcttcca ttgccgacga gtttggcgca aaactccgcg gttccctcta ccatcccgga
    17941 gcccttgttc aggttctctc cgctcccgac ggaatcatct atcctccgct catggacggc
    18001 cggcccgatc cccgcaatgc ccttctcgaa cgcggtacta cccgcatcgg agcgctcact
    18061 tccccacttc tggccgatga gggtcttttc ggacactcca ttgcctcccc gcgcccggcc
    18121 ggcggaacac gtattttcgt tcgggtgttc aatgccccaa acatggccga agcctccttt
    18181 tatggagatt cccaggtttt caccgtagag gacaacaaag tcttcaacgt ccgtattgcc
    18241 cgcaccgaca aggatcttga tccggctgat ctcgatggcg atggtctcag caattcttgg
    18301 gagaaaagcc tcgacaccga tccgggaaat ccggacaccg acggagacgg aatgctggac
    18361 ggcgaggaat tcctcgccgg tacggattca aaggacagca gttccatgct gcagatcgcc
    18421 cgcatcgtcg gcttttcagg cggcggcttt gacatagcct gggacagtgt gcccggcagg
    18481 cagtatcgcg ttgagttcac gccgcatgat ctggcgggcg gcgaagtcgt gttttcaaat
    18541 gcaagcgatg tcgtggccgc cgtggatacc cagacggtcc atacggtgaa tgttcccggc
    18601 gcggcttacg gatatttccg cgtctatctg gtggtcgaat gaggattgtg agatgaacgg
    18661 gcccaaacag cagagaaaaa acttccaatg gttggaactt tttcctcgaa aacttccaat
    18721 ggttggaact ttttcctcga aaacttccaa tggctggaag ttttttttcg ccgccctggc
    18781 cgccctgatg ccgaatatgg ggtttgcctg gggctgggct gttcgagaca ccgcccggca
    18841 gtcgagcgaa aatccgacca tttatctcgg tgattcgatc gaatttcact gggatgtcaa
    18901 tgccgacggc tggggcgcga catacaagaa ggccggtgcc gggaccaaca actcctcgtc
    18961 cggcatgaac tggcaggatc tcacatgggt ggatgaagcc ggggatggat acggcgggaa
    19021 cgagggcatc aaaagcgcat cgttccaggg aacctccgtc gcaacctggt actactccct
    19081 ctggctgggc tgggggggct caatcgggga caacggccac tggtacaatg gaagcagttc
    19141 ctggaacgag gggtcgggat ctttcgtttc cagcagtttc accgtcagcg cgctcggcaa
    19201 tccgaccggc gtttctgccg cgcgcgatgc aacctatccg acatcgcgtg tggacctgag
    19261 ctggacggag tggaacaacc gcaatgtgct gattgtgcgc agaaagggct cttccgtggc
    19321 ctggactccg acccagggca cggcctattc cgacggccag agcctcggga gcgacacata
    19381 cgtcgtggag aattcgcgcg acgggggttc atttgaggac accggcatcg gaaccgcgct
    19441 cgaggccggc acgctctact actataaggt gtattccgaa aactactcct actattcctc
    19501 cggcacggag gttagtgtgc gcacccgccc caacgacgcg acctgggacg gcggcggcgc
    19561 ggacaacaac accctgacgg aagccaactg gaacaacgac ctctatccgg cggccagcgc
    19621 caacagcgtc atgcgctttg cgggatccac gcggaccaca ccgagcgtga cgcacgccgc
    19681 gaacagcgat ttcggcagca tctatttcaa ttccggcgcg tcggcgttca ctctcagcgg
    19741 gaatgccgtg gatattttct cgctgattca aaatgactcg gcgaacgcgc agacgatcca
    19801 gaatgccctt gcgctgaacg gcacggtttc gctgagcacc gtcagcggga acctgaatct
    19861 caacggtgtg ctgagcgggt cctacgggat caagaagacc ggcaccgcaa ccgcggcgat
    19921 cacgaacgcc aacacattca ccggcggcgc atgggtggaa aagggaaccc tgcagctggg
    19981 cggacacacc aacgcgatgg gttcgggcgc cgtcagcgta gggaccaacg ccacgctgga
    20041 tttgacctat ggctcggcca cgctccggcc ggtggccttg aatctttata acgggcaggt
    20101 gacaaaagca acttcgagcg ccacaacctg gcggggtgct ctgaattcct acaacagttc
    20161 aactgtctcg gtttcatcgg gcaatttcta tgtctatggc ggcatgaatc tggcgagcgg
    20221 aacgctggtc ttcaacaacg ccaaccaggg gggaatgagc gggggcacaa tgaccggctc
    20281 gggacatctg accaagctgg gatccggagt ctttcagctg cggcccggaa cgcacagcgg
    20341 aaacatagct ctcagcgaag gcgaaatcag gcagtataca ggaaccatga cggccagcgg
    20401 aaccctgacc ctgtccggag ggacccggta cagttcagat ggaagcacca cccggaccat
    20461 taccaaggat gtagtgatta acggcggcgt gggcattgca gaaaacagca ccggcggaat
    20521 tacgatcagt ggagctgtcg ggctgggcgg cggaacacgc acaataacca actccaatac
    20581 gctgaccgtt tccggtgtgg tttccgatgg aggactggag aagaaggatg ccgggaccat
    20641 gatcctctcc ggcaacaact cctatgcatc gggaacgacc atcacagaag gcacccttca
    20701 gataggggac aacggcacca gcggatcggt tgtcggtaat atcgtgaaca attcggcgct
    20761 ggttttcaac cgttccgata atctgacgta cggagggggg atcagcggca ccggcacgct
    20821 ggacaagcag ggtgccggcg ccctgacgct gacgaacacc agcacataca gcgggaacac
    20881 caccattaca gcgggaacgc tgattcagaa cggaacaaac agcagctcgg ccgttgcggt
    20941 ttcgtccggc acgtcgctcg tgggcggagg cggagtcggc gggctgaccg tgacgggttc
    21001 ggtttatccc ggaaccagcg cggccgccat cggccgcctg acggtgactt cgctgacaat
    21061 gaacgacggt tccgcggctt attttcagat cggcgactgc gacaacacat ctgatcgcga
    21121 ctatatcagc aacagcggat ctgccacgat taatgccacc accaccattt atattgatga
    21181 tgatgaggtg tcgaactggg ataacgccgg cagctacagc tggaatctca tcacgggcgg
    21241 cattctggat gcctccaact ttacactgga cgaaagcctc tgggcgctgg acaaggccgg
    21301 cggatcattt gcgttgtccg ccagtggcgg gaacctggtg ctgaccttca ccccgtcggc
    21361 ggtgccggtc gcggaaatca atgtgctcgg gacaaacagc gtttccatca cggatggcga
    21421 taccaccccg gctcttgccg acggaaccga ctacgggctt gtgctggtcg ccggcggaac
    21481 aaaggaccat acctttaaaa tagagaatac cggtacagcg gacctgactg tttccggcgt
    21541 gacgacaacg ggcacacacg cggcagatta cacggtcatc agctggcccg gaattgtgaa
    21601 tccgtctgca tcctccaatc tgattgtgcg gtttgatccc tcttcgcccg gcccgcgcac
    21661 cgccacgctg catgtgaaca gcgatgacga tgacgaggcc gactacgact ttgtgatcca
    21721 gggcaccggc acctatgttg aaatttccgt cagcgggaat tctcagtaca ttgcggatgg
    21781 cgacggttct ccaacgacag atgatcatac ggactttggc ccggtgggca cgatcggcgc
    21841 gacgcaggac cggattttca ccatatccaa taccggcaat gtggcgatgg gcatcggcaa
    21901 tgtgacgacc agcggaactc atgaggccga tttcacggtg accgagcagc cgtcttcccc
    21961 gcttgcgcca agcaacacca cgacctttac tgttcgtttc gacccgtcgg cgacgggtct
    22021 tcggtcagcg gctctgagtt tcaccaatac agacgacaat gtcagtgacg gactggcgga
    22081 aagcccgttc aatttttcaa ttcagggcac aggggttgcg cccgccataa ccaactatcc
    22141 gggcactctt tccttctcct ccgtgctggg atctgcaccg gccgtgcaat cattcagcgt
    22201 caccaacatc ggactgggat ccatgtccta ttcgctgaca accaacgcct cgtggctgac
    22261 gctgaagccg gtttcgggca cggtggccgg cggggccgga caggtgcata cagcggttgt
    22321 ggccgtggcg gccggccagc agccgggcgt atccaacgcc accatcacca tcaccgccgc
    22381 ggatgcaacc aacagcccga ggaccattgc cgtcagctgg acgatcagcg ccattcccga
    22441 tccgtctgtg gcaaccgcag cggcagacgg caagacgatg attgatctgg cgtggaccaa
    22501 gcatgcctcc tatgatgtca tgattgtgta tcgcgcaggc agcgcttcga ccgcgccttc
    22561 gcagggcaca tcctacagcg tgggcagcgc atgcggcagc ggcacggtca tttacaaggg
    22621 atcaggttca gcgctggagc atgtggtcgc ttccggagtg acgcatcact atgcattcta
    22681 ctcttacaac gggaactatt actcctccgg gctgacagat tccgacacca ccacggcctt
    22741 cgccgccggt gaaatcgtgg aaaccttctc ttataccagc ggagtcacgc tggcctcgtg
    22801 caacgggagc aacctgtggg gcggggcatg gtatggggat accggtttgt tcacgaattc
    22861 tgccggcagc ttttccgagc aggcgaatta tcccgcaccc tccggcaaca aagtgctggt
    22921 gactccggcc aacgacgcga atgtggccgt gtaccgcgcc cttgaccgga cctacaacga
    22981 cggcagaatc tacttcggct atgtcatgaa ttaccagtat gaaggagggg acaagtactc
    23041 cggcctctcg ctctactgga acaacaccga ggagaagctt ttcatcggag agatcggggc
    23101 cgccgaccgg cagctgggaa ttgacagcac gggatcttca gtcacgctca cgaacggagc
    23161 cggaaatgac tatatcatcg tgggttatta tgactggagc gccggcgaag ccagggccaa
    23221 agcctacagg attggatcac acaccgtgcc gacggatgag ccctcctcgt gggatgtgac
    23281 cgtatccaag gccagcaact cggtcggatg ggttaacagc atacgtctgg ccgccggtga
    23341 attcggcggc gggtccggca cgcccggaga ctgctacttc gacgaaattc gcatttcgac
    23401 caactgggcg ggtctgcttc aggcagcccc ttcgactccc gatgacccct ccagccagtc
    23461 cgccacggca gacggctatg aaatggtgcg gctcggctgg actaaaaacg gctctgggaa
    23521 cagcgtgctg atccttcatg acgacagcgc cattgcgaca gacccgaccg atggaaacag
    23581 ctacagcgta ggcgattcca tcggagcggc gaaggtgatc tacaaggggt ctgatacggc
    23641 tgctgaacac atcgttgaag acggttccga caactattac aagttctact cctacaacgg
    23701 ggcgaactat tattcggatg gcgtgaccgc ggatgtctcg atggggtcct acgcctcata
    23761 cgagaatgtg aacccgttca gctacacaaa caatacagct ttcggaacat ccatgaaggg
    23821 cggccagggc tttggcgcga attactgggc ggctaacagc gggacatgga aagcgcagac
    23881 caacaccgga acagcaacgg ccgatacccc gaagttcttt gatttgtccg gctacccgga
    23941 tatggccggc aatctggcgt gggtcgagaa tccgggcgac ggcgggtcag ccacggcgga
    24001 tcgcgacatt gatcctgcaa tcaacagcgg cacgttctat gttgccttca tgatgtccta
    24061 tcagtactac ggatccaata agtgggccgg tctgagcctg atgaacagcg gaaccgaaaa
    24121 agcctttttc ggaaagggaa gcggcggcaa ctggagcacg ctggcggccg ttgccgaggg
    24181 cacaacttac tggtcggcgt atgacctggc cgctttcgcc ggtggaggcg ggaacaccgg
    24241 caatgtctat ctggtggtgg gccggtatga tttcggaaca aaggaaatca agaccaaggc
    24301 gtggagagtg ctcgacagcg aattccccga cacggagccc gaatcctggg atgccagcgg
    24361 cacgctcagc acgggcattg atgcgatcaa ccggattcgg ctgaacgtgg gctcatcttc
    24421 ggccggggcc ggtacaatcg gccgggtgtt tttcgatgaa atccgctatg caaccaactg
    24481 ggccggcctg attgcggtca cctgtccgac atgggcgggg agcaacacgc ttggcagcac
    24541 cagtacgtgg ctgggcgaca cggagagttt cgagttccag tcctatcccg tgggactggg
    24601 tcagacggca ggcattgagt tcgactggtc ccgtgacgga tcgttctcct cttattatga
    24661 cacgttctgg tggaagaacg aaaacagcaa cagttactgg accaatcagg tgcagatgat
    24721 ttctgccggc gtggtgacca gccgttttgt ggcctccggc agcggctgca cacccgcgcg
    24781 caccaataat cagccgatca cggtgcagaa tctcaatccg ccgactgacg cagcagccgt
    24841 tcaggacggc accttcagca acagccagat caatctcacc tggacacgcg gagtttcggg
    24901 aggcgccaag gacacgcttg ttgtgcggca gacggcggat tccggctgga ccgtccccgt
    24961 gaatggaacc acctataacg ccaatgattc gctgggaagc ggaactgtgg tgtatcgcgg
    25021 cgcgctggag gagtttgatg acacgggcct ttccccggat acaacctatt actaccgctt
    25081 ctatgccgag aactggactt actattcgac caactatgat gaagccagtg cggccacagc
    25141 cggcggaagc caggaactgg tcatagacgg cggcgccgcg gactggctgg gtacccccgc
    25201 cacggtctat aatgcatcgg ccggatcgct gggagagttt atctggaccg acaagaaggg
    25261 cgaattgagg gtggacagcg gggaccatcc caacggtgat atctccgagt tccgcgtgtt
    25321 tgcggatgag gactgggtgt atttcctggt gaagatgacc aatattacgg acgaagccaa
    25381 accgtatgtg gtcatcggcg tggacacgcg cagaaattcg gccagcgcgg gcatgaactg
    25441 gctgggtgac gactccggcc tgtttgccgg aggcgactac tttgggggcg gcgcagcggc
    25501 cgtgcactat gccgagtatc agcttaacat ccattccgcc ggcggcgggg ccaaaatcga
    25561 gatgtatgcg aaagacgggg cctactggta tgcgccgccg acggggggag atacgaatgt
    25621 ggcgatcagc acatccggaa atgcagttga actgaaagtc gcgcgcgcgg atcttggtct
    25681 tacaggcgat gttaccgggc ggtttacggt ggcgtccttc ctgaacacag gggcatggaa
    25741 caatgacggc gatggaacgg ttcacattgc cgacaacacg gcggatgcga ttgattccat
    25801 gtccattgcc ccctataaca cggccgacaa ttcggccagt ctgtcggcct ggctggaaga
    25861 catcagcgat gcagacattg atttctggct ggatgtaaaa ttctccggca ccggaatttc
    25921 tgcgaacagc aaggcttcga cgcccgtcct ggtctcgccc acaaacacgg cggctacgag
    25981 cgcaagtccg cagctggtct ggaacaagtc cacggattca gacggcgagg tgacgggtta
    26041 tctgctggaa atcagcacca acgagcagtt caacggtgtg acgggtacag aaaacggaac
    26101 catccagctt cgggtgaatc tggatgcgaa caccaccaac tacacgttta ccacatcagc
    26161 ttcgcagtac tggtggcgcg tgcgcgcccg tgacaccgcc ggggagctgt catccgcggc
    26221 cacccgctcc ttccgtgtgg tcgggaagct cgataccgaa gggcctctgc caactctgct
    26281 ttacatcggc acgaatgtga cgggttatct ggccggagat tacgatacac atattgcaac
    26341 atacggatat atccagagtg tactggacag tgaaatccgc gacacaaaca atgtcttcgg
    26401 gttcgtgata cagtggagcg atgccagcgg agtctatgcc accaaccaaa tgaaggcggc
    26461 ggacagtcct cccaacgggg cgggaggttt tgctttcaac attgttgaca ccgacggccg
    26521 cgtttcgccg aactgggatc tggtggaaat agatacggtg aacggcacca ccaatgacct
    26581 gtggggtgta gatcgaccgt tctatacatc aaacacgctg gcggccggaa acggggatct
    26641 gaaggtgacc aactacgtgc cggcggcttt cacggtgacc aattacgatc cctccattgc
    26701 ctattacctt acactgagcg cggaggacag ctacaccgag ggcggaagct ggtgggatta
    26761 cggatcatgg cccagttttg cctcttcagc ggcgctgccg tactacagcg ggtggtgcga
    26821 agacggcccg aacaccatgc gcaacatcac cacgaattat ctgatcgaag tgcatgtaac
    26881 cgatgatgac atcatcgcgc cggttgccag taccgatctt gcctgggaga atgatgcctc
    26941 catgacggtt tccaattcgc agggcgcgct ggcctatgtg ggcgggagcg gtcaggacgt
    27001 gctgtatcag atcacagacg gcagcctgat aggcgactgg ctgactttca gtttcaacgc
    27061 gtatgagtcc tactattccg gcattgcgct tggcaccagc gaaacattca gtcatctggg
    27121 ccggactctg accaatacgg cctttgtggc ggcatactgg cagaccaact gggccaatta
    27181 cagctcatcg cggagccaga cttcagacac gctcagcgca gatacgatga tcacatggta
    27241 ctggccgacc atcaccacac aggatgtcac caaactctgg ggtcccgaca gtctgtcggg
    27301 agaaatgggc gttacgaaca ttatccagct tgatctgtac gataccgata atgaccgggt
    27361 tgaagatcag gcctcggccc gcgtgaattt cggccgcgtc agactggtgg acgacgatgc
    27421 cgcgccgccc ggcctttctc tgaccagcat cggcggtctg tcggcgcaga acttctacac
    27481 cgaaagcggc gagatgatgt attatgattt tggaacagga gatgccgccg acctgcagcc
    27541 ggcgcggatt ttcacgtccc tttcagttgc agatctgacc gtgctgggcg tcacgccgga
    27601 caccgcggcg ggggaaagcg ggaatgcggt gtcctacggc tactggaaca ccgccaccag
    27661 ctactgggaa tttgccgtga ccgtgcagga cgggttccgg ctgactgtgc agaccattaa
    27721 attcggttcc cgggcgacct cgaccggccc gacagactgg gcgctgaaat acagcggcga
    27781 cggctatgct tccgacctcg catccggatc actgaacaac aatggaagtt ttgaaaccat
    27841 atccgcatcg ctgacatcgg tgaccaacac cggcgcagtg accttccgcc tctatggttc
    27901 cggcgccagc agctacgccg gcacatggcg gctggacaat ctgagctaca ccggaaagat
    27961 tgcgacagta tccggcacct atctggccac ggacggggat cttcacacaa acggtctggt
    28021 gatcaaggcg agcgctcagg acagctacag cggactctat gcgatcacca gtccggacgg
    28081 gccttcctac gatctcatat cgcccgctga agcatcccat aacaatgcgt ttggcgccgg
    28141 tccggcggtg gatggttcgc ccacaaatca ggccgtcacg ctggcggaca ccaatggtta
    28201 tccgtatgcc gatatcgtgc tgggcaccta taccgcggtg gtgcaggtta tggactatga
    28261 cgttgaccgg gatggcgacg gcctgaccgt ggcggtgacg ggtgtggtca ccgttgtgga
    28321 tgatgacacc ggcgcgccga aatacagcac ggcgcgcgga acctatgtgt tcttcgacgg
    28381 cgtcagcgcg ggatcagagg ggaatgccgt gacggacggc atgctgacca acgggctcag
    28441 catcaccaac aggctctatg atgaaaagag cggcatcctg agcaccagcg tgcagttccg
    28501 gctgctggat ccgatcggat gggacagcgg cctgcaggac ttcaccaccg ggccggccga
    28561 cggcgaatcc ctcaccaaca aatttgcgga cgacacggtg gtttcggtgg acggatttga
    28621 cgtggatctc cagaaccgct cgctgggcgt ctggaccacc cttttctacg ccacggactt
    28681 tgatcttgat cggcccggcg atgtgctttc cacgacccag agccttgtaa tgtacgtggt
    28741 ggacgatgac aagctgggcc cgcgcatgac gaatttcctg tccatggggt cggccggggt
    28801 gctcattgcc tcgggcttcg aggtgattga cggatgggag gctcacagcg aggggaactg
    28861 gacggaggag gcctttgacg gcacctggac cggctccaac atgtatgtga acacgctgaa
    28921 cggccgcggc cccgaaggca ctactgcggg ttataatgcg ggtttcaatg atgtgaacga
    28981 ttcgctgcag atgccgccgg tggatggacc cggctgggtt gtgctgtggg cgaaactgag
    29041 cgccgagggc gagtccaaaa tgatgctgga atggctcgat gattccacat ggaccagcgc
    29101 gggcgagctt tccatcacct ccaccggata ttttgaatat gcctggacgg tcggcatgac
    29161 caatgcgggg gtgtcgctga ggcttcgcat gacggagaaa accaccggaa atcgttcaat
    29221 ctatattgat gacatcgccg tcgttccggt caggccctgg accaattctg cggtgtcgct
    29281 ggcgtgggag gcttcttccg acgccgatac cggcgactcc ggcattgatg agtacagggt
    29341 cgtgggcctg aacgccggcg cgccgctcta tactaccaat ggcgccggag tcagcgcgct
    29401 gtctacatcc gtggctccgg cggtggaggg ggtggtcacg ggttatgtct ttgcggtgga
    29461 tgccgatctt gaccgcggct cgattgaccg gacgatgggg ctggcggtgc cgtacatcct
    29521 gaagattgac accacgccgc ccgcccagat tgttgtcacg aatgcctcgg aagggcctga
    29581 cgacacatcc gaaatccgca tcggatggaa tccggcggcg gatgccggaa atccggccct
    29641 ttctccgtgg aagagctatg tggtgtttta cacagatgac ggcggcacag ccgacaccaa
    29701 tgacatgttc atctatgtgg actccggcgg gccttccgtc ctgggtgata ttggcacgcg
    29761 cgaagtgata ctttccaatt ttgtcaacgg ctccacctac aacctggcgg tggccggcct
    29821 ggacgaagcg ggcaacatgg gcccgctggg cggcttcacg aatgtcacgc tggcggtgct
    29881 cagtgtgacg cagtcgctcg ccgaggtgga tactgttgtg accaacggcg tgcatgtgtc
    29941 cttcacggcc accaattcgc agggcatcat gaaccgcaag attgatgtgc tctacgcaga
    30001 tagcctgcag tttgttgaag ccctcaccaa cgaatggacg ctgctgggac tcgctgacag
    30061 caccagcttc ctgattgaca ccggccaggt gtcgggcgtt cctccgcgcc agctcggcgc
    30121 gaacatgatg cgtttctatc gcgcggcttc ccagaacaac tggctgccgg cacgcaaccc
    30181 gcgcagcgcc tccgtcgaaa tcttcggggt caagcccgtc accctgtatc cgggtcaaaa
    30241 ctggatatcg cttcccttct ggccgacccg ccagaatatt ctgcatgtgc tcggcagaag
    30301 cctgcctgcc ggaaccacgg gcggggatga acaggccact cgggtgtcct ggtacgaccg
    30361 caacaacggc gggtggcttg cgacgcagga agtggcgctg attcagtctt cgcccaccag
    30421 tttctggctg tataccgagg gcggcggtgg tggcgacggc aatgatgcga ttctcccgct
    30481 tcatgaggcc tttgtaatcg aactcccaac caatgccgcg ccgcagacca tactgctcac
    30541 cggtcgcgtg ccgaccaact ccatggtgca aacggttcgc gccatgcata acagtgtacc
    30601 cggccgaaac ctggtgtcct tcaatgctcc gcgctatctg catccctcgc agatgggtct
    30661 gctggaggcg ggttttcagg gcggcgccgc gccgatctgg tccgatcaga tatggaagta
    30721 tgaccgctca ggccagtttg ccccgtatcc tgtctggttc cgcacaagcg acaacacctg
    30781 gcgacatacg catccgttcc cgttcccgcc ggtggcgtcc aattactttg ggcctgatga
    30841 cggcattttt attcacaccc gcgcctccac cagcgactgg tcgtggacca acaagattct
    30901 ttacaagctg ccgacctcgc gcatggacct gtaaaaagtt ccaatgactg gaacttttta
    30961 cagaaaaact tccaatggtt ggaacttttc gcggcaaaac ttccaatgat tggaacttct
    31021 gggccggttc aggcccagag cttttcggga tagattccgc tgtcggtcag cgtcctgatt
    31081 tgcgcctgca cgaagggccg gtcctctcga taggttacgc cgaaccaggt tgccggggta
    31141 tgcagaacct tcacacgcat cgccccggcg cgtatcgcgc cggtgaccgc ggcgggaatg
    31201 ccaaactccg cccggggatt cgcgatgtgc tccttcagga agtccgtaaa atgcattcgc
    31261 gtctgttcaa ggaagccggg catgaagccc cagcagttca tcgaaacaat ttcatttccg
    31321 gtcagtttaa ttctttctcc gagcgggctg attccgctga tacccccccc ggtttcgcgg
    31381 atatccgtgt gttcagaggt cccggtcaga aagccttccg cgctggtttg acaaaggccc
    31441 cgcgaaacgg ccccgtgagg cgacagcgtc tggcgcagaa tgaatccgac catgcaggag
    31501 tctgtttctg cgtcgcccga caggtgccgg gccagggcgc gaaaggcctc cgcgccgtag
    31561 aaatcatcgg cgttgataac cgcgaagggc ccgtgcagct gatctgccgc ggaaaggatc
    31621 gcgtgggccg tgccccaggg tttggcccgt tcggcgggcg gtttgaatgg cgcgggaata
    31681 ttctccattt cctgaaaggc gaaatccgcg gccaccggtt ttaaacctat gcgctcgcgg
    31741 aacgcctgct ccatttcgcg gcggatcacg aagaccaccc ggccaaatcc cgcgcgaaca
    31801 gcgtcaaaga tggaatagtc cagaatggtt tccccggacg gcccgacggg atcaagctgt
    31861 ttgaggccgc cataccggct gccgatgccg gcggacagga tcaggaggga gggcttcatt
    31921 gttcagctcc ttttcaactg ccggtcgagg cgtgccgtga tgtcggccgc catgcgctca
    31981 atatagcgct gcacatagtc ggaaatgtcg agatccggat tttgaaccag cggagacaga
    32041 tccattgcaa acgaaacctt tgacgcaata tcctgtgatc ggggatcaaa gaacgtggca
    32101 ttgctcagcc agcggcccac agtggcgcgg ttgtattttt tcccgctgga aatctgcgag
    32161 tcgtgggccc caagcagggc attcgcgatg ttttcgtgtc cggccacaga catgatcacc
    32221 ttgtcggcat cgaggagcca gtcaagactc cgccagactt cgcacccgta aacagtgggc
    32281 ggaacatcgc caacggggct ttcgcgaaga gcttcgatca ccgccacaca gacggcaacg
    32341 tgcgtgtcgt gcttgtcggc cgggttgtga gtgtagataa tggatgggct ggttgtcttc
    32401 agaagctgct tcagatcgtt cttcaaatct tcgctgacgg gattcctgat tgattctgag
    32461 ttgtagtcca gctgagcgac gaagctgtac tggccgatgg cggccgccgt attctgttcc
    32521 cgggcccgga tggcgtgcat ttcctcctct gtgcagtgcg cataggggcc gttgcccggg
    32581 gttccccgtc catccgtgca ggcaatgccg ccgaaccaca gctccttttt gtggagacac
    32641 tggaggatgc cgtgataggc catgatttca atgtcgtcct gatgggcgcc gattccgagg
    32701 tgggtggtgt gcgcaaaggc ctcttcgggc gtgaaatcat cggggaagaa gagcgtcgct
    32761 ccgggattgt tcagcttaat cataatcaac ttcccctgca agcagaagag ccgcctcttg
    32821 cgaagcggct cttccattca cggtttgaaa tctagctcag cttcgctttg gccgccgccg
    32881 cgatggaccc gaaaccctcc gggtcatgga tggcgatttc ggacagcatc ttgcggttca
    32941 gacggatgtc cgccttcttc agcccgtcca tcagacggct gtaggtgatg ccgtgggcct
    33001 tgcaggccgc cgagatacgg accacccaga gcgagcggta atcgcttttc ttgcggcggc
    33061 gatgctcaaa ggacatcgcc atggagcggt ccacggtttc cgtggctgtg cggaagagct
    33121 tgctgcgccc gcccatgaat ccgcgggcga gtttcagcct tcttttccgt ctttttcttg
    33181 aagcgggtgc gttggttgcg cgtggcatgg tttacctcct gaaatcagct gtgaagcagc
    33241 gtggcgatgc gcttgtggtc ggctttggac accatggcgg cggacgccat gctgcgtttc
    33301 tgtttccggc tcttgcccgt cagaatgtgg gcgccgccgg cggaagaacg cttgaacttc
    33361 cccgtgcccg ttttcttgaa acgcttggca atggcttttc tggttttctg cttcggcata
    33421 actgattcct gtttaatatt ctcttccctg aaaacggggg ctatatgtac gacagacccg
    33481 cccgttagtc cagccctttt gcataaaaaa agcaaaaaaa tttgaattac aggctgaaat
    33541 ttccgcccgg actgatataa ggttatatag agatcagaag cattcagcgg gacagactgt
    33601 catgagactc gttttgggcg gagtccgggg caccagcccg gtagcagatc cgaagttcgc
    33661 agagttcggc ggtgacacgt ctgcctttct tgtggagggc tcaaacgggg aggccattgt
    33721 tattgatgcc ggcagcggag tgcgcgtcct cgacccctat ctccgccaga cggaccactc
    33781 ctccgctgtg ctgctcctga tgacccatta ccatcttgac caccttatgg ggttcccttc
    33841 tttttctgtg atatatgact ccacgtggga tttggaaatc aagggtcccc ggctggacgg
    33901 ggttgcggca tcggacgccg tcaggcatat tctttcgcgc ccgttctggc ctcttcagct
    33961 cgaggcgctt ggcgggaaca ttgtcttcac ggaccttccg gaagacggcg gcgcgccgca
    34021 tatctatcac gggctggaag tgcgctggtg tcctgtcccg catctggggg ggtgcgtttc
    34081 gtatcggttg gatgacccgg ccacccgccg cagcgtggtc atagccacgg atgtggaatg
    34141 gcctctggcc tcggagacag agcgccacaa tttcatggag ttctgcaaat atccggctcc
    34201 gccggacctg ctggtttttg acggtcagtt caccccggat aattatgcgc gttttcgcgg
    34261 ctggggccac agcacgtggc aggacgccat tgatgtcgca aagctggtga aagccggcga
    34321 gctgattatc acgcaccatc atccgcgcag cgatgatgaa aagctccggc agattgaagc
    34381 agaggtgaag cggcagatgc ccaatgcatg gctgggccgg caggacgccg caatcgaaat
    34441 cggctgacgc gcctatttcg actgcttgat ccgcagctcg ggatgctcgc tcgccacaaa
    34501 gctgttgggc ggcacggtct ccatcagaaa cacgttgcct ccgatggttg aattgcggcc
    34561 gatcaccgtt ttgcccccca gaatggtggc gttggcgtaa atcaccacat tgtcctccac
    34621 tgtgggatgc cgcttgatgt gcttgacggg ctgcccctgt tcgtcgagat caaagctgcg
    34681 ggcgcccagt gtcacaccct ggtaaatctt gacatgattc ccgatggtgg tggtttcgcc
    34741 aatgacgatg ccggtcgcgt gatccatgaa gaacccgtgt ccaatcgtgg cgcccggatg
    34801 aatgtccacg ccggtctgat ggtggatgta ctcgctcatg atgcgcggga caatggggac
    34861 cttcatgcgg tagagttcgt gggcgagccg atgagaggca atcgccagaa gcccgggata
    34921 agcgagctgc acttccgcat aggtgagcgc cgccgggtcc ccttcatagg cggcccggac
    34981 atcctccacc agcagtttgc gcacatccgg aagccgcttc tgcagtttgc gcaggatgtt
    35041 cacggcctcg cgagcgggat cgcagacttt ttcaacgccc tccgtccgcg cggcctgtcc
    35101 cagccagcgg aagggaatag ccttttccag ttcggggcgg agaagcctcc agccgtgact
    35161 gagccgcctt agcagaaaaa tgcccagttc tccgcgctcg atggtgtccg gggtcatttt
    35221 cccgggaaac atgacgtcga tatagatctt aagggcctca atgatgttgg tggcgttcgg
    35281 ataggatgaa agaacggcct ccggactgtc cttgccgtcg acggagtaca gcgggagcag
    35341 ttccgccacc gcttcgcaga ggctgtgctg gttttcgccg gcgatgcata atttcttctc
    35401 tttggtcata gaaaccgcct ttcaacggaa aacaaggcta ctgcacccgg ctcggcgaat
    35461 caagtttatg cttggacaaa gccgcccgtg cgcataacgt ctgtcctgat catgaaaaga
    35521 ttcgtcaaaa ttgcgctgat tgcggcggca gttctggtgc tcattgtgcc tcttttttcc
    35581 cgaattctgg ccagaaaggc cgttgtctgg gctgtgggcc gagagacggg cctgaatctt
    35641 gaaatcggcc ggctgaaaat agggaagctc ggatcatggt ttgaaatcac agactttcga
    35701 ctgatgaatc cggagggttt tgagcctgga ccctgtgtcg aaatcgccag actgcatgtg
    35761 cgctacagtc cccgcagttt gttttcagac acccttcgca tttcccggct ttatctggat
    35821 atttcccgtg tgctcatcgt ggaggagggc ggggcctcca atcttgaccc gctggtggaa
    35881 aaaactcttt ctgcgccggt ccggccggaa accgcgcgtt cggcacataa atcccgtcca
    35941 gcccaaaccg agccggattc ctcacctccg ccgacaacat cggacggtcg gaaggatgtg
    36001 agcattggcg agctgacgct gaaactgggc acacttgaac tgcgccggcg cgcggcagat
    36061 gaagacgagc cggtgattca ggagtttgtt tttaatgaaa ccgttgttct cacaaatgtg
    36121 gtcagtccgg agcaggttgt tcagcgcgtg gccgccggct ttctggtgaa gcagatggca
    36181 tccggcctgc ttgaaccctg aacaaattct ggtcaacaga cattgacgtc ggccctgtga
    36241 tgagttagtt atcagcggtt ctgaaatcct ctggtcgggg cgtagcgcag actggtagcg
    36301 cgtctgaatg gggttcagaa ggtcccgagt tcaaatctcg gcgccccgac cattttt
//
LOCUS       DAOQWR010000006        35580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1397_length_35580_cov_5.580915, whole genome shotgun sequence.
ACCESSION   DAOQWR010000006 DAOQWR010000000
VERSION     DAOQWR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 35580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35580
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1397_length_35580_cov_5.580915"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..2393
                     /locus_tag="PKK36_01005"
     CDS             60..2393
                     /locus_tag="PKK36_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR93167.1"
                     /translation="MKKAVSVCLVLMTFICTRADEPSLAVKKYLADERIMVAADSMAL
                     VEPKAYGKAPAAEAADLLRSDNMDVQKELRQGVVFIRPELTQSPAAKDIAVQLGAHFS
                     PVLKGRDRRFSIIPLERVVFALKPGVPTDAVAGRLPSAPRQLANGTWSVELTGAENPW
                     ASLELAAALTGLPEVEWAVPDCLRDVQRHYLLNDITNLWYLHNTNVTVNTNSAALPGA
                     WDTTLGSPEIIIAVLDDGTDIAHPDIAPNVWTNTLEIPGDGIDNDANGYTDDINGWDF
                     YAGTNVVSPTHADENHGTATAGLAASRGNNAMGVSGSCPQCTLLPVKLFGGNDMVTDS
                     QWAEAIAYAAGRAKVISMSLGTAAELTAQLVSAINNASTGCLILASSANDGDVGWFET
                     ECPPTSYSGAFYGWLYHSTAVAGTGSHTYRWSYEKDESVSEPYDCAWIDAVTLPNGTT
                     YDFENGIIPAGWTTGGNANWFVSPESADSMVVGAYSLRSGSITHNQSSWLQAVASGAG
                     TVSFYYQVLGEMSCYSDNSGVGIWDGLAFSVDSVEKLWDANQEVESDWGWNPWHVNFP
                     AALTSVVAVGGSTIGGYRSTYSCFGTNMEVTAPTSGSTWEEYIRTTDRVGTNGYDDTS
                     YTDYFSGTSASCPIAAGIAGLIWSGNPELTPHELREIIRGSARKIGYVPYVSGFNQRY
                     GYGQIDAAAAMKLMLSNMPPDFTGIESSGGLVRFRTVGIETARTYHIHSATGLAANGA
                     WNWQDLTPAGVPGAPVFGTNVTPWVTGNVSRVFRIRK"
     gene            2495..3391
                     /locus_tag="PKK36_01010"
     CDS             2495..3391
                     /locus_tag="PKK36_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93168.1"
                     /translation="MQGFPWQVECFTVLLILIGIFLPLILPVHRQVVAGSMPWADSAS
                     QITFAVFDQGYGYLWALVLVGAYFLHWVTAYISPDFVSTPFAYMFSPTIFGALTYGRL
                     LMWSRNFPSELKVVRGSIAEAVIWLVSIHLITLLLARLRMHLHLRKFRDVEWDVDLKP
                     RREWRAVIQLLLKVHPLYFWPMRYMACGEGILIRGVSYIMPVPFAAIESVSMAPRATT
                     ITTSFFLATSVTSLLRIQLYDRLDAVFIGPRHPNLMQQYCAQILAGRLPISERHLIPE
                     GPSTPYGEPVQDGEEYNPYYNI"
     gene            3414..4841
                     /gene="pyk"
                     /locus_tag="PKK36_01015"
     CDS             3414..4841
                     /gene="pyk"
                     /locus_tag="PKK36_01015"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR93169.1"
                     /translation="MRKTKIVATIGPASRHPAMLEKLIAAGLNTARLNFSHGTQAEHA
                     EVIRDIRRISQAAGVPVAVLQDLAGPKIRIGRIAAGAALLRQGEVFVLTNRAVPGDEH
                     AVSHSYPLLPAEVTKGDTILLADGSIELEVAETTSDDVVCRVVIGGPLSSHKGLNLPT
                     SSISVPVLTEKDKLDLRFGLEQGVDYIALSFVRSAEEIRQVRKLIRESGHDTPLIAKI
                     EKHEAVNSLDEIIEAVDGLMIARGDLGVEIPLHRVPLIQKTIIQKANAAGKPVITATQ
                     MMRSMVDSPRPTRAEAADVANAVLDGTDAVMLSEESAVGRYPLETLRCMGQIAEDVEA
                     SLPYDAWLLRHLRDEKFSPEEAVAHAACRLARSIHAAAIATCTTSGSTARLVAKYRPS
                     APVLALTPNARTCAQLALVWGIRPAMMPVAESADELAALARAAVMKSGLAESGQQIVV
                     TAGLPLKQPGTTNMIRVVTLEETAE"
     gene            complement(4816..7113)
                     /gene="ptsP"
                     /locus_tag="PKK36_01020"
     CDS             complement(4816..7113)
                     /gene="ptsP"
                     /locus_tag="PKK36_01020"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNR93170.1"
                     /translation="MAYQNIEEICSIAELASLFERSGSIREFLQTSVSVIAYHMRAAV
                     CSIYLYDEQRQRLVLRANQGLESAMVGKIELRPGEGIVGTAFREMRTLRVARARESSY
                     YKPVPGSGEERYEAFLAVPIMLGTERVGVLVVQDPQADYFTPNDARALKAIAAQLAGT
                     MDNARLFMNMGREEILRAPASAARPVAPVIQGEVAVPGIASGRTFTPRKPYESLLAGY
                     ADADWPSRPEDFERALATTEQEIKKLQRELEEKASDVASMIFSAHLMILKDDAFSGAM
                     RQLITAGMPPHKAIVEVINKYVRLFSGSTNPHLREKTQDVKDLGHRLLSSLRGADAQT
                     HGYEDHLIVATDLLPSDIMRFAAQGAGGLILSGSGSTSHVAILARSLGLPTLLLDEAV
                     VQRVPEDTPVILDANQGNLYISPSAEIMERYGELLAAQQEMRTGEEVPGGPVRTADGV
                     EIRVMANINLISELQPSLRLGADGVGLYRSEFPFIVRNDFPPEEEQYRIYRKILSEMQ
                     GRDVLFRTLDIGGDKMLSYFPHVTEANPFLGLRAIRFSLHHKGIFSEQIRAMLRAGHG
                     FELKIMFPLVSSVDDYLQAAGVVKECLETLRHEGIAHNAAPKLGVMVELPSAVGVIEE
                     LAREADFISIGTNDLVQYMLAVDRTNRLIRDMYQCSHPAILRAIRRIADAAARESTPL
                     SLCGDAAADPVLIPFFVGAGLRTFSVAPYSVPMVRRAIQNLDSCPAREHAEKLLGMGR
                     LEQVNAELSRFAAASGGVTPPSLQG"
     gene            7240..7656
                     /locus_tag="PKK36_01025"
     CDS             7240..7656
                     /locus_tag="PKK36_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93171.1"
                     /translation="MAANGEKAHIMVAEDDPRTRLCLCNILREEGYRVTPSENGYLAL
                     QQINALHLYDGGVDFLILDIHMPGLTGLEVTERLAERRSKIPVIVITGDSDPHLAEGL
                     KQAGCRHIFIKPFDLHQLLQTLKEEKTPNPDQKPVG"
     gene            complement(7638..7880)
                     /locus_tag="PKK36_01030"
     CDS             complement(7638..7880)
                     /locus_tag="PKK36_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93172.1"
                     /translation="MKTRIKRAARIISGWIFLLLGVAGLFLPVLQGILFLAVGAFLLS
                     PYIRFFRKMKCVLYRRFPRSREFAERLRRKFHPTGF"
     gene            7989..9383
                     /locus_tag="PKK36_01035"
     CDS             7989..9383
                     /locus_tag="PKK36_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HNR93173.1"
                     /translation="MSARKQESADTSHHYTAKLWRQTLAALRGRERLAVIRSERLTQR
                     IAGIISAPDGDPAGKARAIELGMAYLEMDDAAKLQFFRTLAKEFDADPAAVEAAGRRL
                     TAATEPEERVAAELELRQALLPPRVELFRQLNLLPDGFRFLINMRADLLPHTRGDASL
                     RKLDEDLKTLLASWFDVGLLNLVEITWDSPASLLEKLMEYEAVHEIRSWDDMKHRLYS
                     DRRCFGFFHSKLPNTPLIFVEVALVNGMAGNIQKLLDPSALPLRPEDADTAIFYSISN
                     TQKGLSGIRLGNFLIKTVVEKLGGELNSLKNFATLSPAPGFMNWLADPAQSQTLTGIL
                     SEKECTSLMDLANNNRAHEGLLALLRTRWHEDRKLARALKEPLMKLCAEYIIRTKRNG
                     HALDPVTHFHMTNGARLERINWLADTSPNGMRQSAGIMLNYKYDPASIAANHAKYATR
                     GYIGTSREARQWLA"
     gene            complement(9458..11254)
                     /locus_tag="PKK36_01040"
     CDS             complement(9458..11254)
                     /locus_tag="PKK36_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93174.1"
                     /translation="MSISGAGAGSTILDGGGTRQILSMTTTEPGALISIAGITFQNGT
                     MTGNGGGLQISTEGAGIRLEDCLVSGCAATAAVSVGGGVMLNTITGLVAVVSCSFMNN
                     ASSANVGGLAIGTSSGTVVVSGCSFISNTVHNSGGSEYYGDAGGLMVYSEAEGASHAV
                     ISSNVFIGNSASGGSNPDGGGLMTYQSGSGSTLWLEANEFTGNTAGLGGGGCILRFNQ
                     SADVTVLQNVFTGNRSVIGSGGGALVYIDEGTLSYSGNLHAGNRTGEDGGGIWISHFS
                     GSAQISNNVFTLNQATNNGGGLFLATDSGSMDVERNVFDSNSSGNSGGAMNMASGTGS
                     VDARNNTFYNNAAVSDGGSLYVYLDQAAAQSDLRNNIFWDSMPNAFGYSYGSGPGTLS
                     LTYSAVESSSGEPWFGTGCITNNPLFRNAPAGDFKLNWTGYPVTNQTKSPCIDSGDPA
                     SALDSDSTRADMGAYSFPQGLAAMGTPHWWLDMHALTAGGLSFDTAEMTDTDSDEALA
                     WREYVADTNPTNSASVFRIFGISAFSQAAVSFTGSVNRLYTLCGCSDLAGGGWTNVPG
                     AGPRVGAGGADTMLDTNDPPAGLFYRLRVQLP"
     gene            complement(11602..14223)
                     /locus_tag="PKK36_01045"
     CDS             complement(11602..14223)
                     /locus_tag="PKK36_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93175.1"
                     /translation="MKTLRIALFVFFALITSSAFAQITMPDLLNYQGRLIDGTNLVNG
                     DVEITIHLWDAPTGSGSGWPGCTDSSTVHVVDGLYSTYIGDDVSFGSLDNALNQTQVW
                     VEVIVGTNVLSPREQLMSSAFARYAAKMPANAVTMDMIAPDQIQSWHIASGQVRSVNI
                     QVGAVSNAQIAAGAVTSDKIGTWAISNVNLAFGSVSSSVIEDGGVGNLDIAAGVVRSN
                     HINWAAMPAGLQDGDDGMAYTAGTGLVIVGSTIAVSNGGVGSVQLASGSVSSNKIDWA
                     SMPSGLQDGDDTGVSLASYDENGTFSPAPAASGSDSIAIGRGSMARADHSVVSGGRDN
                     IITNLADYSAIGGGYANRVGNLANYTTVSGGNDNRAEDSASWTTIAGGGLNRIRNNAW
                     YASIGGGYQNTIDTNAQSATIAGGYCNQINREARNATIAGGLQNTIYSSATNASIGGG
                     SGNTISNRSSFAQISGGHNNAIGTNSPNSVIAGGYNNYIRAEAGSASIAGGVNNYIAY
                     AAELSAIGGGSGNSIGSRSYYSVIGGGNVNQIGSGSEYGVISGGGSHSIGNASPFMFI
                     GGGNFNTTGTNCGYAVVAGGQMNDLRDNCYYAVISGGRDNDVSEMAEYSVVAGGEDND
                     VGMSSHHAVLAGGENNEIADEAFYSAIGGGANNLIETLARGSVIAGGIGNNLRRNANY
                     SVIAGGDSNIINTNSNASAIGGGANNSIDSSAQCATIPGGYECKVATNASSAFAAGTH
                     AVAQHPGAFVLTDSQMTDFASAGPDTLNVRFQGGVYGTYPRPNYDSGWRVIGMGDTTN
                     LVHNLGGDPDNYVVDLQFNTSTAYGHNQGSGVWTDFADRDVGATWRSLTTTNVVVYRA
                     TSDSRAEEIRLRIWVYQ"
     gene            complement(14752..16617)
                     /locus_tag="PKK36_01050"
     CDS             complement(14752..16617)
                     /locus_tag="PKK36_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR93176.1"
                     /translation="MKAIQVVLFVCFALTITFAQAQGPLAPSGAPEATMKTLDQVEAR
                     IPISQIPYTCEVAGASYYLTGDLTGIAGESGILISAPDVTLDLNGFTLYGDSGSGDTG
                     IMGDSGADNLRIFNGTVRDWGSTFYGVDCAGEYVAVSGVRAFNNGGGIMLSDNSSAVD
                     CIAANNSSAGIIVRHNGRVENCVAVSNGWHGIYGWKSNVVRNCKASQNSSSGIELQGG
                     GHMTDNTSLNNGTGLRITVSGTYVAGNIVKGNAVNYDFADGNQLNILLCEIPETLDWP
                     CTAVLAGSLTCSTVGTNGVSIRSDNVTLDLAGHELIGPGVGSGMGIYCGYGNGTIMNG
                     VVRNWQGGLGIGAFGRGASIKNMHVTDNNEGITIRNAALIESCQINGNLDDGVVIDGS
                     GNIIRNCRISTNLSEGIVFDTFNENLRGNVIENSTVTQNGASGLKFYSTAGDFTGILI
                     KNCTFSYNAASGILFVGHLSSAYFEGISMLDCVFSGNNGAAVSGTATGACHGLVIDSC
                     NFDNNLAGLELARCSGSRIVNNTFSRNAGSGIYLTSSTANRIDGNTIYGWGGDYGIYT
                     DEASCSDNLIVRNQSGSHTTDFSISSADTYGPVVTAAGELDTTGSATHPWANFDL"
     gene            complement(17003..17767)
                     /locus_tag="PKK36_01055"
     CDS             complement(17003..17767)
                     /locus_tag="PKK36_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR93177.1"
                     /translation="MNFPLIHNIHYTWTGWPTPDISFPPEPDAAFFASLVLLWAKDGL
                     ELVSREWKQNLLHMSFRAEPGVSPVFCASRVKGRLQHALRAKGLPNAFSRKVSVRALG
                     INVSDIVTRYLRNQTVHADYADPRYLRQFSDAAEEDASLDLSQPIETGSGRYWYNLHM
                     VLVTENRVKIGKEDFLPKIPAAVEKWASGSGCAVKSVAVMPEHIHLLFRGAPDKSPAE
                     LAESCWSILNRGAGCRLYSDRIYIGTCSEYTLRAVL"
     gene            complement(18030..19820)
                     /locus_tag="PKK36_01060"
     CDS             complement(18030..19820)
                     /locus_tag="PKK36_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type lectin domain-containing protein"
                     /protein_id="HNR93178.1"
                     /translation="MGTNVLSPREQLLPAAYAQYAKNLPANAVKSWHIDSDQIYSYHI
                     PDGELRGNKLRNGTISNAQLAANAVASNVINWANMPAGLQDGDDGETYTAGTGLDLVA
                     NEFSIADEGVSSNLIDWTTMPFGLEDGDDVLTAGTGLAVVGGVISITNDSIGDDQLKK
                     SYQSGSVPISIPPYASATTVPVTFSPAFDTMPIVTASLNLNPMPDDLSASVAIISQDT
                     SGFEAKVVSSGDSFGQWTSGITVTTNTVPADARNSICEVNGRPAVAFMRTMSSSDWRL
                     YYTRANNSAGTSWPSGARIGVHTGVYAVAMCMVNGNPAIAYLDVYSSGIYYVRATDSS
                     GSAWSAPIQITVNARFGQPSLNIVNGNPAIAYIGKTNELRYVRASNANGSAWNTPVWV
                     DSPTNYLMQDSLFVADGRPAVITRVKEGAGYNEPFLFLRAHDADGTAWGDYAFIGATD
                     SPEVWVEGFSCAVIDGYPSVAYVNTDDYKIYYRRAIYPDGSAWFEEGVPVTGLFSGGY
                     LSLAEIGGKPAVCYSDLLAGLFYSCAPNVEGTAWGAPQRIGSQDISLSSTMENINGMP
                     MIAYGNNSTGVSFAVATATTGTLNWIAVEP"
     gene            complement(19839..20141)
                     /locus_tag="PKK36_01065"
     CDS             complement(19839..20141)
                     /locus_tag="PKK36_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93179.1"
                     /translation="MKALRFVLLVGFALITTSAFGQGIDIPNLLNYQGRLVDGTNLVN
                     GNVEMTFHLWDAPTGSGMGWPGCTDSGTVSVVDGLYSTYIGDDVTFGSLDNALNQT"
     gene            20604..21284
                     /locus_tag="PKK36_01070"
     CDS             20604..21284
                     /locus_tag="PKK36_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93180.1"
                     /translation="MKIRMLFALAVLALISLNVMGQYSQPSTCLDGAGDRSSGGAYSN
                     ISAVAQPGGTAVSSAGGMVNYAGFLGTFSLQPGLDTDADGLEDEVDSDNDNDALADLS
                     EIEGSEFSPATASNPNLADSDDDGITDYGEAVAGTDPTDENAHLQIVSITENAGNKEI
                     AYVAREGKTYRIRAAGEDFSGGSAEMDTQTVGAWNADDWSVHTNVYTDTTAPSNTLLY
                     AIEPVPGP"
     gene            complement(21378..21791)
                     /locus_tag="PKK36_01075"
     CDS             complement(21378..21791)
                     /locus_tag="PKK36_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93181.1"
                     /translation="MKTMKVLIITAAMAAGSAAAQSCGNDARSSLGFTITSDGRVGFS
                     YSYDSNPPYVVCSPPQVVYCPVPRVVYYSSRPAVYWPSKPVIIRTPPRTLQGWHHDRQ
                     GFSKPSGFRPPHSAHGDRRPPPKPPDSKHRDRARR"
     gene            21927..22547
                     /locus_tag="PKK36_01080"
     CDS             21927..22547
                     /locus_tag="PKK36_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR93182.1"
                     /translation="METEQTFPLNETLRSIEQRRSVRVFLDQPVPQECIRAVLRAANQ
                     APSAHNQQSWRFIVVSGGKKDELARLVSARAGNFSRPATALLRMAARSIDSAPVVIAV
                     ANTGQLIAHGTELFKVGAEEAHDFFRTMEIQNSAAAAENLLVAATSLGLATVWLGILY
                     LIKDEVLHFLGEPKGEFMAVIPIGYAARMSSGPAKVPLEQVVRRLQ"
     gene            complement(22616..24301)
                     /gene="rpsA"
                     /locus_tag="PKK36_01085"
     CDS             complement(22616..24301)
                     /gene="rpsA"
                     /locus_tag="PKK36_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNR93183.1"
                     /translation="MAAMYEETLKSFSEGSIIPGKIIEIRDNAVLIDIGYKSEGLVPI
                     SEFREPETIKVGDTIEVLLQKLEDDSGMVVLSVQLAEQQRNWDRVFSSFEEGGIIDGK
                     LVHRVKGGMIVDVGVEAFLPGSQLDIVPVRNADELLGQTLQFKILKINKERRNIVLSR
                     RELLEDQRRSQRKDLLADIKIGQSRVGVVKNITDFGAFIDLNGMDGLLHITDMSWGRI
                     NHPSEMLSVGQEIEVLILDVNMEKERISLGLKQKTPNPWDGIAQRYPVGTRITGPVVN
                     IMPYGAFVEIEQGVEGLVHVSEISWTKRVAKASDELAVGDIVEAIILSVSAEDKKISL
                     GIRQTESNPWDEVQDKYPIGARVSGKVRNFTTYGAFVELEQGIDGMIHVSDMSWTRKV
                     NHPSEVVNKGQDVECVVLEVDASNQRISLGLKQAQEDPWAALTSKYSIGQIVTGKVTK
                     MAPFGAFVELAEGIDGLVHISQISEERVANIRDVLNVGDEVTARIVKIDPAERRIGLS
                     IKAAALPDEEFSVSDDMLQGLQPGENLVDLAGAFDQVFGDSNVKPEEWHPGQK"
     gene            24586..25968
                     /gene="hydA"
                     /locus_tag="PKK36_01090"
     CDS             24586..25968
                     /gene="hydA"
                     /locus_tag="PKK36_01090"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNR93184.1"
                     /translation="MSLLIRNGEIVTPDSRYCADIFCEGETITRIGRGLEAPPEAEVI
                     DAQGKYVFPGFIDPHVHVYLPFSGTFTRDTYQTASRAALLGGTTCFIDFISPTNKEDL
                     LESVFIWHGKAAGQSACDYTFHMTVPRWDSGAESQLREIVASGIPSFKVYLAYKSSTG
                     LDDPGLFHTLKLAKELGVVVASHCENATIVEELQRQFIRSGQTGPEWHYWTRPPLVEA
                     EGVNHLLTFAELHGTHTYIVHTSCREALEAAVRARRRGVQTWIETCIHYLLLDKTRAE
                     QGGFEAAKYVISPPLRDKDQQADLWLALRDGVISTLATDHAPTDFATQKALGKDDFTK
                     ITNGVPGIEDRINLLYTYGVCPGKIDLHTFVDVASTQPAKIFGMFPRKGAIQPGGDAD
                     LVIYDPNFKGTISAKMHSMNVDYNAYEGREILGRPDTVTVRGQVAVRNGVFTGSCTHG
                     RFVKRDPSHF"
     gene            26048..28012
                     /locus_tag="PKK36_01095"
     CDS             26048..28012
                     /locus_tag="PKK36_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93185.1"
                     /translation="MNSTSAPSWTKLLLVLSFAALACTGIRTVSNDAVWTHLAAGKAG
                     LVKTDTLSFTREGEDWINATWLYDRILNAVWSDSNPWLAVLIHVALVIAAFLLLLPAA
                     REWGGPVSISLSLLLSTWLLAPRFEVSPALAGVFFAALFIALLEKERSLWVLPAALIP
                     AQILWTNMQEGFLIGPALCMVYTVEAFQFDAATRAARGKNPPVVLAAMTVILLAATLI
                     NPSGLRLHGAVIASWTSAAAAFTGTWISPFNAPAQSITRHLVTLAMLTGAAGLLMQRK
                     KLPTALTTAAVFSAFVLIFFRLKLDSFIWFALLAFPFFCLSLSAVGESFGGFLERRKN
                     ANPAALQARTGIVALLLVLFTIFQFITNRFYNAEQSCSRFGFGVEQDVFPSQALAVIG
                     RAGFPAKAVNMVRDGAYLAYHLPERKVFTDGRGDLYGAAFYQALNLALRGDAEQWEAL
                     LAKWDPEALILNMCDPSASSITHHLLMTGKWMLAYFDGTTAIFATPASGLHEKIAEEG
                     LQAEGLKSIDRAFAGDENGGGLIRRPVSARLAGAGQLFLNMRRYPQAEAVYERLTKSV
                     PDFAQAWYNLGISQLSQEHADKAAKSFDRVVRLQRKNALAWLWLSLAHAVLGNQAEAR
                     YAFTKGKRLNSTLAASFGDPLATGDSTEKP"
     gene            28021..30222
                     /locus_tag="PKK36_01100"
     CDS             28021..30222
                     /locus_tag="PKK36_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNR93186.1"
                     /translation="MLETGAIGPRQPAEHLEGPVERVTFHSEETGFAVLKVKAKGHRG
                     LVTVVGQLPNVVPGEIVQAQGTWLHDQKHGRQFKSESIRTVPPESIDGITRFLGSGLI
                     RGIGPVYAGKLVEAFGKDIFDVIENRSALLERVEGIGRMRRLRIKESWGETRSVRAIM
                     SFLMSCGVSTARAFRIYRVYGDQAIQQVKKDPYCLARDIHGIGFLTADKIAEKMGIAR
                     DSELRARAGVEYALMELTQQGHCACPREQLQEETARLLQIDECLAGEAIDHGLAKGRL
                     VAGNQDYNRELVYLASLHCSETALAAHLSALAAGPHPCPGILVDKATAWAEEKTKISL
                     SDDQRRALAAAITAKVLVITGGPGVGKTTLINAVIRILSAKKMQVVLCAPTGRAAKRM
                     TESTRHPAKTIHRLLAYDPKSRRFRFNDKNPLAGDIFIVDEASMIDLPLAAQLISAIP
                     GHASLILVGDVDQLPSVGPGNVLKDIIASGMFPVHRLTQIFRQATHSEIVLNAHRINQ
                     GLPAEPGEPGSESDFQFIERDDPEQVLKTLVGLVETSIPNRYRLDRLSDIQILTPMKR
                     GLLGADHLNRRLQQALNPRPEGIERFGVRYCVGDKVMQQQNNYDKDVFNGDCGVISAI
                     DEGAREMIVNFDGRPVSYDLQELDELLPSYAITIHKSQGSEYPCVVIPVHTQHYIMLQ
                     RNLLYTAITRGKKLVILIGSRKALAIATRTAEAGRRVTTLAQRLQHRDQPF"
     gene            30314..31075
                     /locus_tag="PKK36_01105"
     CDS             30314..31075
                     /locus_tag="PKK36_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNR93187.1"
                     /translation="MGRIKIRKAFQQAVRPLLLGMRRMTREEILVLGDSHATVFEHRN
                     FKKAFPRHFFNVVSVRGATVSGLSNPMSKTQAMPIFMKALKRSNAKTSIVLLGEVDTG
                     FLLWYKAQKHSAPVEIMMDRAIENYQNLLRTVAAKSKVVCISAPLPTIRDDQDLGEIA
                     NLRKEVKATQRQRTELTLEFNRRMQVFCEENGLRHVSLDREALGPDGLVSEHLLNTNP
                     REHHYDREKHAGLIIRHLLNCAEFSGGSETSVRGQ"
     gene            complement(31058..31720)
                     /locus_tag="PKK36_01110"
     CDS             complement(31058..31720)
                     /locus_tag="PKK36_01110"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNR93188.1"
                     /translation="MAAGTAAAGEPLEFKPLPYAFDALEPHIDARTMEIHYTRHHQAY
                     FNNLLKAVEGTEMAGRSLEDILASADALPAAVRNNGGGHWNHTMFWQVMSPEGGGEPE
                     GALAEQIIRDFGSFAEFQKKFNEAAATRFGSGWAWLCVAEDGSLFVISTPNQDNPLMK
                     AADRRGTPILGLDVWEHAYYLKYQNLRASYIEAFWNVVNWPEVASRYAAAGQKSESLS
                     PD"
     gene            31898..33064
                     /locus_tag="PKK36_01115"
     CDS             31898..33064
                     /locus_tag="PKK36_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNR93189.1"
                     /translation="MKYNFNECPDRRPTESEKWLQYPEDVLPLWVADMDFVSPEPVIR
                     ALHERVDHGVFGYPRSRADLKELIVGRMQERYGWCIHAEDVLLIPGVIRGFNMACRAA
                     GPDGEALVQTPIYPPMLCAPGNAGLARRDNELPASDCSNEIDWADFEAALRGDVRVFL
                     LCNPHNPTGRVFREEELLRMGELCLKHDVIICSDEIHCDLIYSGQTHIPIASLSAELA
                     AQTITLMSPSKTYNIAGLDCSFAIIQNPELRRRFNRARAGLVGGVNIFGQAAAAAALK
                     DGQEWLDQVLAVLESNRNFLFEYVGREWPGVKMFLPEATYLAWLDCRGLNLENPYEFF
                     LSRARVAFNDGASFGKGGEGFVRVNFGCPRKTLEEALARCTAALREAGLIPRKS"
     gene            complement(33059..33559)
                     /locus_tag="PKK36_01120"
     CDS             complement(33059..33559)
                     /locus_tag="PKK36_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93190.1"
                     /translation="MRDKRFIAAHRGGPLSREDHVLLARWAADCAEGVLHLFTRSSDD
                     ERPRAALDTARRWANGEVKTGAAMKASLAAHAAARGAGDKAAVAAARAAGQAVATAHF
                     ADHCMGALLYAMKALETSGVSADSEFRLQISKLPARLREPVSEGVLLRFRKLGIRRPG
                     PPHGRS"
     gene            complement(33809..34864)
                     /gene="ric"
                     /locus_tag="PKK36_01125"
     CDS             complement(33809..34864)
                     /gene="ric"
                     /locus_tag="PKK36_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HNR93191.1"
                     /translation="MMKNIFKEVPYTEGRMGSRQLVNEKPLLVMQIALKPGQQVPLHK
                     ANSNVHLLVLEGDVIVTLDGREFPAPRGTLLPVAFQTPMTIRNDTDSNASFIVLKTPN
                     PSEMSSSGISVREDQTVAELVVEHPELKEKLEKMGIDYCCGGKRPLGEAVRAAGIEWP
                     AFVSKLQAEAKSSAQKDWNKVPVTELADHILNRHHTFMKEQLPRMDSLLTKVRSAHGE
                     NHGPMIDDLRRVFDSLRAEIEMHLMKEEQILFPAIKAVDAYVSGTGGVPEIHCGSVAN
                     PIRQMEYEHDSAGEALSEMRRITGNYTLPEDACQTFAALYDGLQAIEADLHEHIHLEN
                     NILFPRSVRQEKEMQEG"
     gene            complement(34940..>35580)
                     /locus_tag="PKK36_01130"
     CDS             complement(34940..>35580)
                     /locus_tag="PKK36_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR93192.1"
                     /translation="VLAALGANLMLTPGAEGMKGAISRAESIAASDPKKFFMPQQFKN
                     PANPAIHESTTGPEIWNDTDGAIDVLVSGVGTGGTITGVSRFIKKTKGKKILSVAVEP
                     EESPVIFQKLAGQELKPAPHKIQGLGAGFIPENLDLSLVDRVEKVSSAEAITMARRLA
                     REEGLLTGISSGAAAAVAARLAASEEFAGKTIVAILPDAGERYLSTVLFEGL"
BASE COUNT         7398 a        10798 c        10203 g         7181 t
ORIGIN      
        1 gcattttttg gtgctatttt gtgtcatgat ttgatagaaa tctccaaaag tgaggtcaca
       61 tgaaaaaagc ggtttccgtt tgtcttgtcc tgatgacttt tatttgcaca cgcgcggatg
      121 agccctccct cgcggttaag aaatatctgg ctgacgagcg catcatggtc gccgccgaca
      181 gcatggccct cgtggagccg aaggcctacg ggaaggcgcc ggcggccgag gccgcggacc
      241 tgctccggtc ggataacatg gatgtgcaga aggagctccg gcagggtgtt gtcttcatca
      301 ggcccgaact gacgcagagc ccggcggcaa aggatatcgc cgtgcagctc ggcgcgcatt
      361 tcagcccggt cctgaaaggc cgcgaccggc gcttcagcat cattccgctc gagcgcgtgg
      421 tctttgcgct gaagcccggc gttccgaccg atgccgtggc cggccgcctg ccctccgccc
      481 cgcggcagct cgcaaacggg acgtggagcg ttgagctgac cggcgccgag aatccctggg
      541 ccagcctgga actggcggca gcgctcaccg gcctgcccga agtggaatgg gcagtgcccg
      601 actgcctgcg cgatgtgcag cgccactatc tcctcaacga catcaccaat ctctggtatc
      661 tccacaacac gaacgtcacg gtcaacacga actccgccgc actgcccgga gcatgggaca
      721 caacgctagg gagcccggaa atcatcatcg ccgtgctcga tgacggcaca gacatcgccc
      781 atccggacat tgccccgaat gtctggacca acacgctgga aattcccggg gacgggattg
      841 acaacgacgc caacggctac acggacgata tcaacggctg ggatttctat gcgggaacca
      901 atgtggtgtc gcccacgcac gcggatgaaa accacggaac cgccacggcg ggcctggcgg
      961 cctcgcgcgg gaacaacgca atgggcgttt ccggatcgtg cccgcagtgc acactgcttc
     1021 cggtgaaact gttcggcgga aacgacatgg tcaccgactc gcagtgggcc gaagccattg
     1081 cctacgcggc gggccgtgca aaagtcatca gcatgtcgct gggcacggcc gccgaactta
     1141 cggcccagct tgtcagcgcg atcaacaacg cctccacggg atgcctgata ctcgccagct
     1201 cggccaacga cggcgatgtg ggctggtttg aaacggaatg tccccccacg tcctacagcg
     1261 gcgcgttcta cggctggctg taccattcca ccgccgtggc cggcaccggc tcgcacacct
     1321 accgctggtc ttacgaaaag gatgaatcgg tctcagaacc ctacgactgc gcatggatag
     1381 acgccgtcac gcttcccaac ggcaccacct acgactttga aaacgggatc ataccggccg
     1441 gctggaccac cggagggaac gcaaactggt tcgtgtctcc cgaatccgcc gactccatgg
     1501 tggtcggcgc gtattccctg cggtcgggct ccatcacgca caaccagagc agctggcttc
     1561 aggctgtggc aagcggcgca ggaaccgtct ccttctatta ccaggtgctg ggcgaaatga
     1621 gctgctacag cgacaacagc ggcgtgggga tctgggacgg gctggcgttc agcgtggaca
     1681 gtgtcgaaaa actctgggat gccaaccagg aagtggaaag cgactggggc tggaatccgt
     1741 ggcacgtcaa tttcccggcg gcgctgacct cggtggtggc ggtcggcggg agcaccatcg
     1801 gcggctaccg ctcgacctat tcctgcttcg gcaccaacat ggaagtgacg gcgccgacat
     1861 cgggaagcac ctgggaggag tatatccgca caaccgaccg cgtcgggacg aacggatacg
     1921 acgacaccag ctacacggac tacttcagcg gcacatcggc ctcctgcccg atcgccgcgg
     1981 ggatagcggg cctgatctgg agcgggaatc cggagctgac gccgcacgag ctgcgggaga
     2041 tcatccgcgg cagcgcgcgc aagatcggct atgttcccta tgtctccggc ttcaaccagc
     2101 gctacggcta cggacagata gacgcggccg cggccatgaa gctgatgctg tccaacatgc
     2161 cgccggattt caccggcatt gaaagcagcg ggggcctggt ccggttccgg acggtcggca
     2221 ttgaaaccgc gcgcacctat cacattcatt ccgccaccgg gctggcggcg aacggggcat
     2281 ggaactggca ggatctgacg cctgcgggcg tgcccggcgc gccggtgttc ggcaccaacg
     2341 tgacgccgtg ggtgacgggc aatgtctccc gcgtgttcag aatccgaaaa taacggacgc
     2401 aggcaataga gcttgccctg cgggcgccga ttggtttagt ttgcatcccg tgacaacaaa
     2461 ggagccgaga aaaacatatc actcccggtg ggaaatgcag ggctttccgt ggcaggttga
     2521 atgcttcacg gtcctgctga tcctgatcgg catttttctg ccgctgatcc ttccggtgca
     2581 ccggcaggtg gtggcgggat ccatgccgtg ggcggacagc gcatcgcaaa tcacctttgc
     2641 ggtatttgac cagggctacg gctatctgtg ggcgctggtg ctcgtcggcg cgtattttct
     2701 gcactgggtc acggcttata tttccccgga tttcgtttcc acgccgttcg cctacatgtt
     2761 ttcgccaacg atcttcggcg cgctgaccta cggacggctc ctgatgtggt cgcgcaactt
     2821 cccgtccgaa ctgaaggtgg tgcgcggcag catcgcggaa gccgtcatct ggctggtttc
     2881 cattcacctc atcacgctgc tgctggcgcg gctgcgcatg cacctgcacc tgcggaagtt
     2941 ccgggatgtg gaatgggacg tcgacctgaa gccccgccgg gaatggcgcg cggtgatcca
     3001 gctcctgctg aaagtgcacc cgctgtattt ctggccgatg cgctacatgg cctgcggcga
     3061 gggtattctg atccgcggcg tcagctacat catgccggtt ccgtttgcgg cgattgaatc
     3121 cgtatccatg gcgccccggg ccaccaccat caccacctct ttcttcctgg ccacaagcgt
     3181 aacatcactg ctgcgcatcc agctgtatga ccggctggac gcggtgttca tcggaccgcg
     3241 gcatccgaac ctgatgcagc agtactgcgc gcagatcctc gccgggcgcc tgcccatctc
     3301 cgagcgtcac ctgatcccgg aagggccgtc caccccgtac ggcgaaccgg tgcaggacgg
     3361 cgaagaatac aacccctatt acaacatctg atccatccgg acggagggcg gacatgcgca
     3421 agacgaaaat cgtggcgacc atcgggccgg cgtcgcgtca tccggccatg ctcgaaaagc
     3481 tgatcgcggc cggcctgaac acggcgcggc tcaatttttc gcacggcacg caggccgaac
     3541 acgcggaggt gatccgcgat atccgccgga tctcgcaggc agccggcgtc ccggtggcgg
     3601 tgctgcagga cctggccgga cccaagatcc gcatcggccg catcgccgca ggcgccgcgc
     3661 tgctccggca gggcgaagtc ttcgttctga cgaaccgcgc cgtgccgggc gatgagcatg
     3721 cggtttccca ctcctatccc ctgctccccg cggaagtgac aaaaggcgac accattctgc
     3781 tggcggacgg atccattgaa cttgaagtgg cggaaaccac ctcggatgac gtcgtgtgcc
     3841 gcgtggtcat cggcgggccg ctgagctcgc acaagggact gaaccttccg acaagttcca
     3901 tcagcgtgcc tgtgctgaca gaaaaggaca agctggacct gcgcttcggc ctggaacagg
     3961 gagtggacta cattgcgctg tcgtttgtgc gcagcgcgga agagatccgg caggtgcgca
     4021 agctgatccg ggaaagcgga cacgacacgc cgctgatcgc aaaaatcgaa aagcacgagg
     4081 cggtgaacag cctggatgag attatcgagg cggtggacgg actgatgatc gcgcgcggag
     4141 acctgggcgt cgagattccg ctgcaccggg tcccgctcat ccagaaaacc atcatccaaa
     4201 aggccaacgc cgcaggcaaa ccggtcatca ccgccacgca gatgatgcgg tccatggtgg
     4261 acagtccccg gcccacgcgg gccgaagcgg ccgatgtggc caatgccgtc ctggacggca
     4321 cggacgcggt catgctctcc gaggaaagcg cggtcgggcg ctatccgctg gagaccctgc
     4381 ggtgcatggg gcagatcgcc gaggatgtgg aggccagcct tccctatgac gcgtggctgc
     4441 tgcgccatct tcgggacgag aagttcagcc cggaagaggc cgtggcgcat gctgcctgcc
     4501 ggctggcgcg cagcatccac gcggcggcca ttgcaacctg caccacatcc ggcagcacgg
     4561 cgcggctggt cgccaaatac cggccgtcgg cgccggtgct cgccctgacg cccaatgccc
     4621 gcacctgtgc gcagctggcg ctggtctggg gcatccgtcc ggccatgatg cccgtcgcgg
     4681 aaagcgccga tgaacttgcg gctctcgccc gggcggcggt gatgaaatcg ggtctcgcgg
     4741 aaagcggaca gcagatcgtg gttacggcgg gccttccgct gaaacagccg ggcaccacca
     4801 acatgatccg ggtggtcacc cttgaagaga cggcggagta acaccccccg aagcggctgc
     4861 aaaccgcgac aactcggcgt tcacctgttc cagcctgccc atgccgagca gtttttcggc
     4921 atgctcgcgg gcggggcatg aatccaggtt ctggatcgca cgccgcacca tcggcacgga
     4981 atacggcgcg acgctgaacg tgcgcaggcc cgcacccacg aagaagggaa tcaggaccgg
     5041 gtccgccgcg gcatcgccgc agagcgacag cggcgtgctc tcgcgggcgg ccgcatcagc
     5101 aatccgccgg atcgcccgca ggatggcggg gtgactgcac tggtacatgt cgcggatcag
     5161 gcggttggtg cggtccacgg ccagcatgta ctgcaccaga tcattggtgc cgatggagat
     5221 gaagtcggct tcgcgggcca gttcctcaat cacgcccacc gccgaaggca gttccaccat
     5281 aacccccagt ttcggagcgg cattgtgcgc gatgccttca tgcctgagtg tttcaagaca
     5341 ttccttcacc accccggcgg cctgaagata gtcgtccacc gatgacacca gcggaaacat
     5401 aattttcagt tcaaagccgt gcccggcccg gagcatggcg cggatctgtt cgctgaagat
     5461 tcccttgtga tgcagcgaga agcggatcgc gcgcagcccg agaaacggat tggcctcggt
     5521 cacatgcggg aaataggaca gcattttgtc gccgccgata tcgagcgtgc ggaaaaggac
     5581 atcgcggccc tgcatttcgg aaagaatttt ccggtagatg cggtactgct cctcctccgg
     5641 aggaaaatca ttgcgcacga tgaagggaaa ctccgaccgg taaagcccca caccatcagc
     5701 cccgagccgc agcgagggct gcagttcgct gatcaggttg atgttggcca tcacgcggat
     5761 ttccactccg tcggcggtcc gcaccgggcc gcccggcacc tcctctcccg tccgcatttc
     5821 ctgctgggcc gccaggagtt ctccatagcg ttccatgatt tcggcggaag gggagatata
     5881 aaggttgccc tgattggcat caagaatcac aggggtgtct tcaggcaccc gctgaacaac
     5941 cgcttcgtcc agcagaagcg tgggcaggcc cagcgaacgg gccagaatgg ccacatgcga
     6001 ggtggatccg ctgccggaca ggatcagtcc gccggcaccc tgcgcggcaa agcgcatgat
     6061 gtccgacgga agcagatcgg tggccacgat gaggtgatct tcgtagccgt gggtctgggc
     6121 atccgcgccg cgcagactgg acagcaggcg gtggcccaga tccttcacat cctgtgtttt
     6181 ttcacgcaga tgggggttgg tgctgcccga gaacaggcgg acatacttgt tgatgacctc
     6241 cacaatcgcc ttgtgcggcg gcatgccggc cgtgatgagc tgccgcatgg cgccggaaaa
     6301 ggcatcgtcc ttgagaatca tcagatgcgc gctgaagatc atggaggcca catcggaggc
     6361 cttttcctcc agttcccgct gaagcttctt gatctcctgc tcggtcgtgg caagggcgcg
     6421 ctcaaaatcc tcgggccggg aaggccagtc ggcatcggca tatccggcca gcagagattc
     6481 atagggcttg cgaggagtga aggtgcggcc gcttgcgatg cccggaacgg cgacttcgcc
     6541 ctgaataacg ggcgccacgg ggcgggcggc cgaagccggg gcgcggagaa tctcctcgcg
     6601 acccatattc atgaagagcc gggcgttgtc catggttccg gccagctggg cagcaatggc
     6661 tttcagggca cgcgcgtcat tgggcgtgaa gtaatccgcc tgaggatcct gtaccacgag
     6721 cactcccacg cgttccgtgc ccagcatgat gggcaccgca agaaaggctt cgtagcgctc
     6781 ctccccggat cccggaaccg gcttgtaata ggagctttcg cgcgcgcggg ccacgcgcag
     6841 cgtgcgcatc tcccggaacg ccgtgccgac aatgccctcg cccggccgca gttcgatttt
     6901 cccgaccatg gcggactcca gaccctgatt cgcccgcaga acaaggcgct ggcgctgctc
     6961 atcgtaaagg tagattgaac agacggccgc gcgcatgtga taggcaatca cgctgaccga
     7021 agtctgcagg aactcgcgaa tgcttccgct ccgttcaaac agactggcca gctcggcaat
     7081 actgcagatt tcctcgatat tctgataagc cataacacca gaccccaaaa acaaccggcg
     7141 ggaaagtact ccccgaacct gcggcacgca agcagtcttt tcacataatt cagtccacat
     7201 ggcccgcttt atgctctgat atccgaagga gatggcacca tggccgcaaa cggggaaaaa
     7261 gcgcatataa tggtggcaga ggacgatccg aggactcgtc tgtgcctgtg caatatcctg
     7321 cgcgaggagg gctaccgggt gacgccctcc gaaaacggct atctggcgct tcagcagatc
     7381 aatgccctgc atctttatga cggcggggtg gattttctca ttcttgacat tcatatgccg
     7441 gggctgaccg gcctggaagt gacggaacgc ctggcggagc gcaggagtaa aatcccggtg
     7501 atcgtcatca ccggagacag tgacccgcat ctggcggagg gtctgaagca ggcgggttgc
     7561 cggcatattt tcatcaagcc gttcgatctc catcagctgc tgcaaacgct gaaagaagag
     7621 aaaacgccga atccggatca aaagcctgta gggtgaaatt tcctccgcag gcgttcagca
     7681 aactcccgcg agcggggaaa acggcggtac aggacacatt tcattttacg gaagaagcgg
     7741 atatatggag acagcaggaa tgcgcccacg gccaggaaaa ggatgccctg cagcacgggg
     7801 agaaaaagcc cggccacacc aagcagcagg aaaatccatc cgcttatgat acgggcggcg
     7861 cgctttattc tggtcttcat gggtccatca tcccttgtgc agtataacgc atcggccggg
     7921 ggcggtccag ctgcagagga tattgcattt ccagaaatta tgatatggtc ttcaaaggag
     7981 ataccgccat gagcgcaaga aaacaggaat ccgcagacac ctcacatcac tacaccgcga
     8041 aactctggag gcagacactg gccgccctga gaggtcgcga gcggctcgcg gtgatccgca
     8101 gcgaacgcct gacccagcgg atcgccggaa tcatcagcgc tcctgacggg gatccggcag
     8161 gcaaggcgcg cgcgatcgag ctcggcatgg cctaccttga gatggatgat gccgcgaaac
     8221 tccagttttt ccggaccctt gccaaagagt tcgacgcgga tcccgcggcc gtggaggcgg
     8281 ccggacgccg gctgaccgcc gcgaccgaac ccgaagagcg ggtggccgcc gaactggaac
     8341 tgcgccaggc gcttttgccg ccgcgcgtgg agcttttccg gcagctcaat ctgctcccgg
     8401 acggctttcg tttcctcatc aacatgcgcg ccgacctgct gccgcacacg cgcggggatg
     8461 cctccctgcg caaactggac gaagatctca agacgcttct ggcctcatgg tttgacgtgg
     8521 gcctgctgaa cctggtggag atcacgtggg actcgccggc ctcgcttctg gaaaagctca
     8581 tggagtacga ggcggttcat gagattcggt cctgggacga catgaagcac cggctctatt
     8641 cggaccggcg ctgcttcggc ttcttccaca gcaagctccc gaacacgcct ttgatcttcg
     8701 tggaggtcgc actggtcaac ggcatggccg gcaacatcca gaaactgctg gatccaagcg
     8761 cgctcccgct gcgcccggaa gatgccgaca ccgccatttt ctattccata tccaacaccc
     8821 agaaagggct ctccggcatc cggctcggga atttcctgat caagactgtc gtggagaaac
     8881 tgggcgggga gctgaacagc ctgaagaact ttgcaaccct ttcaccggcg cccggattca
     8941 tgaactggct ggccgatccc gcgcaaagcc aaactctgac cggcatcctc agcgaaaagg
     9001 aatgcacctc gctgatggac ctcgccaaca acaaccgcgc gcacgaaggg ctcctcgcgc
     9061 tgctccgcac acgctggcac gaggaccgca aactggcgcg cgcgctgaaa gagccgctga
     9121 tgaagctatg cgccgagtac atcatccgga ccaagcgaaa cggacacgcg cttgatccgg
     9181 tcactcactt tcacatgacc aacggcgcgc gcctggagcg catcaactgg ctggcggaca
     9241 cctcgccgaa cggcatgagg cagtcggcgg gaatcatgct gaactacaaa tacgacccgg
     9301 ccagcatcgc ggccaatcac gcaaaatatg ccacgcgcgg atacatcggc acgtcgcgcg
     9361 aggcgcggca gtggctggcc tgaagcgcgc ctcgccgaac cgtcttggcg gattggcgga
     9421 agccggcgat caaagcagac atggaggaag cggcggatta aggcagttga acccgcaggc
     9481 gataaaacag acccgccggg ggatcgtttg tatctaacat cgtatctgcc ccgccggcgc
     9541 cgacccgggg tccggcgccg gggacattgg tccagccccc tcccgcaaga tcgctgcagc
     9601 cgcagagggt atacagccgg ttcacggatc cggtgaagga aacagccgcc tgagaaaatg
     9661 cggatatgcc aaaaatgcgg aatacagaag cgctgtttgt cggatttgtg tcggcgacat
     9721 attcgcgcca ggccagcgct tcatcactgt cggtatcggt catttctgca gtgtcaaatg
     9781 acaaaccacc ggccgtgagc gcgtgcatgt ccagccacca gtgcggcgtg cccatggccg
     9841 ccagcccctg cgggaacgaa tacgccccca tgtcggcccg tgtactgtcg gaatcgagcg
     9901 cggatgctgg atcgcctgaa tctatgcagg gcgatttggt ctggttggtc accggatatc
     9961 ccgtccagtt cagcttgaaa tcgcccgcgg gggcgtttcg gaaaagagga ttgtttgtga
    10021 tgcagcccgt gccgaaccag ggctcgcctg atgagctctc aactgcagag taggtcaggc
    10081 tcaaggtccc gggcccgctg ccatacgaat atccgaacgc attgggcatg ctgtcccaga
    10141 aaatgttgtt gcgaagatcg ctctgggccg cggcctggtc cagatacacg tacaggcttc
    10201 cgccgtcgct cacggctgca ttgttgtaaa atgtgttgtt gcgcgcatcc accgaaccgg
    10261 ttcccgacgc catgttcatc gctccgccgc tgttgccgga actgttcgaa tcaaatacat
    10321 tgcgctcaac atccatgctg cctgaatccg tggcaaggaa aagaccgccg ccgttgtttg
    10381 tcgcctgatt caatgtgaat acattgtttg aaatctgcgc gctgccgctg aaatggctga
    10441 tccagattcc gccgccgtcc tctccggtgc ggttgccggc atgaaggttt cctgaataac
    10501 tcaaggttcc ctcatcaata taaaccagag caccgccgcc gctgccgatc accgagcggt
    10561 tacccgtgaa cacattctgc agcaccgtca catcggccga ctgattgaat cgcagaatgc
    10621 atccgccgcc gcccagaccc gccgtgttgc cggtgaactc gttggcctcc agccacaggg
    10681 ttgatccgct gcccgactgg taggtcatca gcccgccgcc gtcgggattg cttccgccgg
    10741 aagcactgtt tccgataaaa acattgctgc tgatgaccgc gtggctggcg ccttctgcct
    10801 ctgaatatac catcagaccg cccgcatcac cgtaatactc gctcccgccc gaattatgca
    10861 ctgtgttgct gataaatgag caaccgctca ccacaacagt tcctgaagaa gttcctatcg
    10921 ccaaaccgcc cacattcgcc gaggatgcgt tgttcatgaa actgcaggac accaccgcca
    10981 ccaggcctgt aatggtattc agcatgaccc cgccgcccac gctgacagcg gctgtcgcgg
    11041 cacagccgct gaccagacaa tcctcaagcc ggatgcccgc gccctccgtc gaaatctgca
    11101 ggccgccgcc gttcccagtc attgtgccgt tttgaaaggt gatgcccgcg atggagatca
    11161 gcgctccggg ttcggtggtc gtcatcgaaa ggatctgcct ggtgccgccg ccgtccagaa
    11221 tggtggaacc cgcgccagca ccggaaattg acatggtacg gttttccgcc gagttaaagt
    11281 tcagcgtcgc cgtcagggca tatgtttcag ccgctgcctg tataaaatca tcctcaccgt
    11341 ttgcggcagc cgaatccaat gccgtctgga actccaaagg cgtcgaaaca ttgaacgtcg
    11401 cagccgatgc ggttaagcag agcagcgcca tggtaaaagt tccaatgatt ggactcgagc
    11461 ccggcgagag gggctttccg aatgggaagc aatgcctgaa aggcatggca acttttcctg
    11521 caaaaagttc caatgattgg aagtttttca gcgcattttt ccaatcattg gaagttttca
    11581 tggatgtctc acgttttgaa atcactgata gacccagatg cgcagacgta tttcttccgc
    11641 acgggaatca cttgtggctc tgtacacaac cacattggtg gttgtcagac ttcgccatgt
    11701 tgcgcccacg tccctgtctg caaaatcggt ccagaccccc gatccctgat tgtggccata
    11761 cgccgtagat gtattaaatt gaaggtctac aacatagtta tccgggtcgc cgcccagatt
    11821 atggacaagg tttgttgtat cgcccattcc gattaccctc cacccactgt cgtaattcgg
    11881 acgcggatag gttccgtaaa ctccgccctg gaaacgcaca ttcaatgtgt cgggaccagc
    11941 gctggcgaaa tccgtcatct ggctgtcggt cagaacaaaa gcaccgggat gctgcgccac
    12001 agcatgcgtg cccgccgcga acgcactgct ggcgttggtg gcgactttgc attcatatcc
    12061 gccgggaata gtggcgcact gtgcactgct gtcaatgctg ttattggcgc cgcctccgat
    12121 cgcacttgcg ttggaattcg tattgataat attgctatct cctcccgcaa tgaccgaata
    12181 atttgcattc cgccggagat tatttccaat cccacccgca atgaccgatc ctcttgccag
    12241 ggtttcgatc agattattcg ccccgccgcc tattgcggaa taaaaggcct catcagctat
    12301 ttcattgttc tcgccgcccg ccagcaccgc atgatgtgaa gacatcccaa catcgttatc
    12361 ctcaccgccg gccacaacgc tgtattccgc catttccgac acatcattat cacgaccgcc
    12421 gctaataacg gcataatagc agttatctct taaatcattc atctgtccgc cggcaacaac
    12481 tgcatagccg caattagtgc cggttgtatt aaagttgccg ccgccaatga acataaaagg
    12541 tgaagcattt ccaatcgaat gagaacctcc gcccgaaatc acaccatact cgctgccgct
    12601 tcctatttga ttaacattgc cgccgccaat cacgctgtag tagcttcgag aacctatgct
    12661 attgccactt cctccgccaa tcgccgataa ttcagccgca tatgcaatgt aattattgac
    12721 tcctccggca attgaagcag aaccggcctc tgctctgata taattattgt atcctccggc
    12781 aatcacactg tttggactgt tcgtacctat cgcgttgtta tgtccgccgc tgatctgagc
    12841 gaaactggag cgattgctga tcgtatttcc cgagccgccc ccaatgcttg cgttggttgc
    12901 cgaggaatag atagtgtttt gcaaaccccc ggcaatagtc gcatttcttg cttcgcggtt
    12961 aatctggttg cagtatcctc cggcaattgt ggccgactgc gcgttggtgt ctattgtgtt
    13021 ttgataaccg ccgccaattg acgcatacca ggcattgttc cggatccggt tcaacccgcc
    13081 gcctgctata gttgtccagc ttgccgagtc ttcagcgcgg ttgtcatttc cgcccgagac
    13141 tgtggtgtag tttgccagat tgccgacccg gttggcatag ccgcccccga tggcggaata
    13201 atcggcgaga ttagtgatga tgttatcccg gccgccggaa accacgctgt gatcggcacg
    13261 cgccatggaa ccgcgaccta tggcgatcga gtcggaaccg gacgcagccg gagcggggct
    13321 gaacgtcccg ttttcatcat aactcgcgag agaaaccccg gtgtcgtcac catcctggag
    13381 acctgatggc atactggccc aatctatctt gttgctggaa accgaaccgg aagccagttg
    13441 cacagaacca actcccccgt tggatacggc aatcgtactg ccgacaatca caagtcccgt
    13501 gcccgctgta taggccatgc cgtcgtcgcc gtcctgaagg ccggcaggca tggccgccca
    13561 gttgatgtga ttactgcgta caacacctgc cgcaatatcc aggttcccaa ctccgccatc
    13621 ttcaattacg gaacttgaaa cactcccgaa ggcaagattc acgttcgata tggcccacgt
    13681 tccgatcttg tcgctggtta ccgctccggc ggcgatctgg gcgttggaaa cagctccaac
    13741 ctgaatattt acactgcgca cttgtccgga tgcaatgtgc cacgactgaa tctgatccgg
    13801 cgcgatcatg tccatcgtca cggcattggc cggcattttt gcggcataac gcgcaaaagc
    13861 ggatgacatc agctgctcgc ggggggaaag cacattggtt cccactatca cctcaaccca
    13921 cacctgtgtc tggttcaacg cattgtccag actgccaaaa gacacatcat cgccaatgta
    13981 ggttgaatac aatccatcga cgacatgaac tgttgatgaa tctgtgcatc cgggccatcc
    14041 tgaaccgctg ccggtcggcg catcccacag gtgaatggtt atttccacat cgccattcac
    14101 caaattggtt ccgtctatca agcgcccctg ataattcagc agatcgggca tggtgatctg
    14161 cgcgaaggca gatgaggtga tcaatgcgaa aaatacaaac agcgcgattc tcagcgtctt
    14221 catgctattc tcctcctacg ggacgctccc cggcccgatg ccggcgagta taaaagttga
    14281 acgcagctgg atacaatcag gaaatacaaa atagttggct tcgcccgacc tgattcaccc
    14341 gcgacgcaat ccgactgctg gaggatgggt taattccatg tccaatatta tcaatcgctg
    14401 tgtagtttag aatgttgcag gaatgggaaa tatctattcc gtcatcaaga ttttcaaata
    14461 ccaggcaatt ctgcacagta caaccatatc cagtcataat gccccgagtc cgattgtggg
    14521 aagctatgca attccacacg cttcttctat ttgcgatgta gatatcggct tcatcgttat
    14581 gagttgaggt acactcataa accgcgccat tctcgccagt tcctatgccg gatactttgc
    14641 tgaccgcctg cagctcatat tacacagaac cccgtgctga aaagatgcaa accggcgtcc
    14701 cggtgaaatt ttatagaccc ttcctctata acgggcgtgc acagccattt gttagagatc
    14761 aaaattcgcc cagggatggg ttgcgcttcc tgtggtgtcc agctcacccg ccgctgtgac
    14821 gacggggccg tatgtgtcgg cagaactgat gctgaaatcg gtagtatggg atccagattg
    14881 gttgcgtaca atcaggttgt cactgcaact cgcctcgtct gtataaattc cataatcgcc
    14941 accccagcca taaatagtat tgccatcgat ccgattagcc gtgctgcttg tcagataaat
    15001 accggaaccg gcattgcggg agaacgtgtt gttgacaatg cggctgccag aacatctggc
    15061 gagttcaaga ccggcgagat tgttatcgaa attacaggag tcaataacca gtccatggca
    15121 ggctccggta gccgttccgg aaacagcggc gccgttgttc ccgctgaata cgcagtccag
    15181 catggagatt ccttcaaagt aggcagaaga taggtgtccg acgaagagaa tgccggacgc
    15241 ggcgttgtaa gagaatgtgc agttttttat aagtatcccc gtgaagtcac cggcagtgct
    15301 gtaaaacttc aacccgctgg cgccgttttg ggttaccgtg ctgttttcaa taacatttcc
    15361 gcggagattt tcattaaagg tgtcaaaaac aatgccttcg cttaagtttg ttgatattcg
    15421 gcagttgcgg ataatgtttc ctgatccatc gattaccacc ccgtcatcaa gatttccatt
    15481 aatctgacaa ctttcaataa gagcagcgtt acgaatggta ataccttcgt tgttatcggt
    15541 cacatgcatg tttttaatac tcgctcctcg tccgaaagcg cctatcccca aaccgccttg
    15601 ccagttgcga acaacaccat tcataatcgt gccgtttccg tagccgcagt atattcccat
    15661 gccactgcct acacccgggc ctatgagttc atggccagcc agatcaagag taacattatc
    15721 agaacggata gagactccgt tcgttccgac tgtgctgcag gtcagggaac ccgccagaac
    15781 ggctgtgcac ggccagtcca gtgtttccgg aatttcgcac agaaggatgt tgagctgatt
    15841 accgtcggcg aagtcatagt taaccgcgtt ccctttgacg atattgcctg caacatatgt
    15901 tccgctgact gtgatacgca ggccggttcc gttgttcagg ctggtgttgt ccgtcatatg
    15961 tcccccgccc tgaagctcaa taccggagct gctgttttga gaggctttgc agtttctgac
    16021 cacattgctt ttccacccgt aaatcccatg ccatccgttg ctcacggcca cgcagttttc
    16081 cacgcgtccg ttgtgccgaa cgatgattcc ggcgctgctg ttgtttgccg caatacagtc
    16141 cacagcactg gaattgtccg acagcatgat tcctccgcca ttattgaacg cacggactcc
    16201 ggagacggcc acgtattctc ctgcgcaatc gacgccgtag aaggttgatc cccagtcgcg
    16261 caccgtgccg ttgaagatac gaagattgtc ggccccggaa tcccccataa tgcccgtatc
    16321 tccgcttccg ctgtctccat aaagagtgaa cccgttcagg tccagcgtga catcgggagc
    16381 agaaatcaaa atcccgctct ctcctgcgat cccggtcagg tcacccgtca gatagtagga
    16441 cgctccagcc acctcgcagg tgtacgggat ctgggagatc ggaatgcggg cctccacctg
    16501 gtccagggtt ttcatcgtcg cctccggagc gccggacggg gccagcggcc cctgcgcctg
    16561 tgcgaaagta atggtaagtg cgaaacaaac aaacaggacg acttgaattg ccttcatgcc
    16621 actctcctcc ttcgggacgc tccccggccc gatgccggcg agtttaaaat tagaaagcgg
    16681 ccggatgcaa tcccgaagta cacaaaactg ctccgcctga cctgattcac ccgcgacaca
    16741 aggtcgcggg ggtccaggtc gccaaaatct tcgcccccgg cgggctacaa ggtcgcgggg
    16801 gtccaagtcg ccgaaatctt cgcccccagc gggctacaag gtcgcggggg tccaggtcgc
    16861 cgaaatcttc gcccccggcg ggctacaagg tcgcgggggt ccaagtcgcc gaaatcttcg
    16921 cccccagcgg gctacaaggt cgcgggggtc caggtcgccg aaatcttcgc ccccggcggg
    16981 cttgtcccgc cggagcgaaa ggtcacagca ctgcgcgcag cgtatattcg gaacatgtcc
    17041 cgatatatat ccggtcgctg tacaaccggc atcccgcgcc tcgattgaga atgctccagc
    17101 agctttccgc cagctccgca ggagatttgt caggcgctcc ccgaaacagc agatgaatgt
    17161 gttccggcat gactgccacg gatttcacgg cacagccgct gccggatgcc cacttctcca
    17221 cagcagccgg aattttcggc aggaaatcct ctttgccgat tttcacccgg ttctccgtca
    17281 caagaaccat gtgcagattg taccagtagc ggccgctgcc ggtttcaatg ggttgtgaca
    17341 gatcaagcga tgcgtcctcc tccgctgcat ccgaaaactg cctgagatat cggggatcgg
    17401 cataatccgc gtgcacggtc tgattccgca aataacgagt aacgatatcg cttacattaa
    17461 tacccagtgc cctcacagag accttgcggc tgaacgcatt tggcaagccc ttggcgcgca
    17521 atgcatgctg caggcggcct tttacgcggc tggcgcaaaa aacaggcgac accccgggct
    17581 cagcccggaa actcatgtgc agcagattct gtttccactc gcgagataca agctcaagcc
    17641 cgtccttagc ccacaacaga acgagcgacg caaaaaaagc cgcatcgggt tccggaggga
    17701 aagagatatc tggtgtgggc caaccggtcc atgtgtagtg gatgttgtga atgagaggga
    17761 aattcatgcg caataatctg aacctgggtt ggggcccggt caagtccgat ttcaaccgac
    17821 ctggttcacc cgcgacgcaa ggtcgcgggg gtcttcatca gatccgcgac ggtcttgccc
    17881 aacctgagtc acccgcgact caaggtcgcg ggggtccagg tcgccaaaat cttcgccccc
    17941 ggtgggctac aagatcacgg gggtctggtt tgcggagata ttcgcccccg gcgggcttgt
    18001 cccgccggag cgaaaggcca tgtgccaatt tacggctcga cggcgatcca gttcagcgtg
    18061 ccggtggtgg ccgtggcgac tgcaaaactt acgcccgtgg aattattgcc gtacgcaatc
    18121 atcggcatgc cgttgatatt ttccatggtg ctgctgagtg aaatatcctg tgagccgatc
    18181 cgctggggag caccccacgc agtaccttct acattcggcg cgcacgaata aaaaaggccg
    18241 gcgagcagat ctgaatagca gacagccggc ttccccccaa tctccgctaa agacagatag
    18301 cccccgctaa aaaggcctgt aaccggcacg ccttcctcga accaagcaga accgtcagga
    18361 taaattgcac gccgataata aatcttgtag tcatccgtat tgacataggc cacggaggga
    18421 tacccgtcaa tcacagcaca ggaaaatccc tccacccata cttcgggcga atccgtagca
    18481 cctataaatg cgtaatcccc ccatgccgtg ccgtctgcat cgtgagcccg caaaaacaaa
    18541 aaaggctcgt tgtatccggc cccctctttc actctcgtaa ttaccgcagg acggccatca
    18601 gcaacaaata gtgaatcctg cataagataa tttgtaggag aatccaccca tacaggcgtg
    18661 ttccacgcag aaccgttcgc atttgaagcg cggacatagc gcagttcatt tgtcttgcca
    18721 atatatgcga tggccggatt cccgttgaca atattcaggg atggctgccc gaaacgcgca
    18781 ttcactgtga tctggatcgg cgcactccag gcacttccag atgaatctgt agctcttacg
    18841 taatatatcc cactggagta tacgtccaaa tatgcaatgg ccggattccc gttcaccatg
    18901 cacatggcaa ccgcatatac ccctgtatgc acaccaatac gtgcgcctga cggccacgag
    18961 gtgcccgcgg aattgtttgc ccgggtgtag tagagcctcc aatcagaact actcatcgtg
    19021 cgcatgaatg caacagcagg ccgcccattc acttcgcaaa tcgaattgcg agcatctgca
    19081 ggaacagtat tcgttgtcac tgttatgccc gacgtccatt gcccgaatga atctccgctg
    19141 ctgaccactt ttgcttcaaa acccgaagta tcctgtgaga tgatcgctac agatgctgac
    19201 agatcatccg gcatcggatt cagattcagg ctggctgtta cgatcggcat tgtatcaaat
    19261 gccggcgaga aagtgacggg aacggtcgtt gcgctcgcgt aaggcggaat cgatatcgga
    19321 accgatcccg actggtatga cttcttcagt tgatcatccc caatggaatc attggttatg
    19381 gagatcactc ctccaaccac agccagaccg gtcccggcgg tgagcacatc atcaccatct
    19441 tcgaggccaa agggcatggt ggtccagtcg atcagattgc tggagactcc ctcatctgca
    19501 atggaaaatt cattggccac cagatcaaga ccggtgccag ccgtataggt ttccccatca
    19561 tcaccatcct gcaggccggc aggcatgttc gcccaattga tcacattgct ggccacggcg
    19621 tttgccgcca gctgggcatt tgaaattgtg ccgttcctga gcttgttccc ccgcagctcc
    19681 ccgtccggta tgtggtagct gtatatctgg tcactgtcaa tgtgccatga tttgactgcg
    19741 tttgcgggca ggtttttggc atactgcgca taggccgccg gaagaagctg ctcgcggggt
    19801 gaaagcacat tggttcccac gatcacttcg atccagacct aggtctggtt cagggcattg
    19861 tcgagactgc caaacgtcac atcatccccg atataggtcg agtagaggcc gtccaccacg
    19921 ctgaccgtgc cggagtccgt gcatccgggc catcccatgc cggagccggt gggcgcatcc
    19981 cacagatgaa aggtcatctc cacattcccg ttcacgaggt tggtgccgtc caccagacgg
    20041 ccctgataat tcaacaggtt cggaatatct ataccctgac caaatgcaga tgtcgtgata
    20101 agtgcaaaac caaccaacag gacaaagcgc aatgctttca tattattctc ctccttcggg
    20161 acgctccccg gcccgatgcc ggcgagtata aaagctgaaa gcgactggat acaaccctga
    20221 aatcccaaaa agtttgctct cccgacctga ttcacccgcg acgcaaggtc gcgggggtct
    20281 ggcttgcgga gatcttcgcc ccggcgggct acaaggtcgc gggggtctgg cttgcggaga
    20341 tcttcgcccc cggcgggcta caaggtcgcg agggtccagg tcgccgaaat cttcgccccc
    20401 ggcgggcttg ccccgccgga gcgaaaggtc aggacaagag ggagtgctcc agcccatcca
    20461 ctcaacccga cctgattcac ccgcggcgca aggccgcggg ggtctgaagg atttccaact
    20521 tttcatatat ttgggcttgc gctccaaatg aaaagctgac aaatatccag catcgggatt
    20581 aatttcgtac agggaggtat gcagtgaaga tcagaatgct ttttgcctta gcagttctcg
    20641 ctttgatttc cctgaacgtc atggggcagt actcccagcc gagcacctgc ctggacgggg
    20701 ccggcgaccg gtcgtccggc ggcgcctatt ccaacatcag cgccgttgcc cagcccggcg
    20761 gaacggccgt atcctccgcg ggcggcatgg tgaactatgc gggattcctc ggcacgttct
    20821 ccctccagcc cggactcgac acggatgccg acgggctgga agacgaagtg gattccgaca
    20881 acgacaatga tgcgctagcc gatctgtccg aaattgaggg cagcgaattt tctcccgcga
    20941 cggcctccaa tcccaacctc gccgacagcg atgatgacgg gatcacggat tacggcgaag
    21001 ccgtggcggg aaccgatccc actgatgaga acgcccatct ccaaatcgtt tccatcaccg
    21061 aaaatgccgg gaacaaggaa atcgcttatg ttgcccgcga gggcaagacc taccggattc
    21121 gcgccgcggg agaagacttc tccggcggat cagccgagat ggacacgcag accgtggggg
    21181 cctggaacgc agacgactgg agtgtgcaca ccaatgtcta cacagacacc accgccccca
    21241 gcaacacgct gctctatgcg atagagccgg tgcccggtcc ataaccataa agttccaatc
    21301 attggaagtt cgccgggaaa aagttccaac cattggaagt tttctcgtga aaagttccaa
    21361 tcattggaac tttttattca gcggcgggcg cggtctctgt gtttcgaatc gggcggtttg
    21421 ggcggcggcc tgcgatcgcc atgcgcgctg tgcggcgggc ggaaaccgga cggcttcgag
    21481 aaaccctgac ggtcatgatg ccatccctga agggtccggg gcggcgtccg gataatcacc
    21541 ggctttgaag gccagtaaac cgccgggcgc gaggaataat acaccacccg gggcacggga
    21601 cagtatacca cctgcggcgg tgaacagacc acgtacggcg gattggagtc atagctgtac
    21661 gagaatccga cccggccgtc cgatgtaatc gtgaaaccca gggacgagcg ggcatcattc
    21721 ccgcatgact gggcggccgc gctcccggcg gccatcgccg ccgtgatgat taatactttc
    21781 attgttttca tgtcatactc ccttcttctg atcataaaac ccgcacggcg cggttttatt
    21841 gcagggaata tgcaggctga atcgggcatt gagtttcccc tccgacccgc tacgatgagg
    21901 cgctccaggt agaaaggatc catgtgatgg aaaccgaaca gacttttccg ctgaatgaga
    21961 ccctccgctc aattgaacag cgccgcagcg tgcgcgtgtt ccttgaccaa cccgtgccgc
    22021 aggaatgcat ccgggctgtt ctgcgcgccg cgaaccaggc gccgtcggcc cataaccagc
    22081 agtcgtggcg gttcatcgtg gtcagcggcg gcaagaagga tgaactggcc cggctggtgt
    22141 ccgcgcgcgc ggggaatttt tcgcggcccg cgaccgcgct gctgcggatg gccgcgcgca
    22201 gcatagacag cgcccccgtg gtcatcgcgg tcgccaacac cggccagctg atcgcgcacg
    22261 gcaccgagct gttcaaggtc ggcgcggagg aagcgcatga ttttttccgg accatggaaa
    22321 tccagaattc ggccgccgcg gcggaaaacc ttctggtggc ggccacatcc ctcggactgg
    22381 ctaccgtgtg gctcggcatt ctctacctga taaaggacga ggtgctgcat tttctgggcg
    22441 aaccgaaagg tgaattcatg gcggtgattc ccatcggtta tgccgcccgg atgagctccg
    22501 gtccggccaa ggtgcccctc gaacaggtgg ttcggcgcct gcagtagcgg acgcccggaa
    22561 aaacagaaac ggtgacggcg gatgccgtca ccgcttctgt attctcaggc cggaattact
    22621 tctggccggg gtgccactcc tcgggcttca cgttgctgtc gccgaagacc tgatcaaagg
    22681 ctccggccag atccaccagg ttttcacccg gctgcagccc ctgcagcatg tcgtcgctga
    22741 ccgagaattc ctcgtccggc agcgcggcgg ccttgatgct caggccgatc cggcgctcgg
    22801 ctggatcaat cttcacaatg cgggcggtga cttcatcgcc cacgttgagc acgtcgcgga
    22861 tgttggccac gcgctcttcg ctgatctgac tgatgtgcac gaggccgtca atgccctccg
    22921 caagctcgac gaaagcgcca aagggggcca tcttggtgac cttgccggtg acgatctggc
    22981 cgatgctgta tttggaggtg agcgccgccc acggatcttc ctgggcctgc ttgaggccca
    23041 ggctgatgcg ctgattcgag gcatccactt ccagcacgac acattcgacg tcctgcccct
    23101 tgttcaccac ctcggaagga tggtttacct tgcgggtcca ggacatgtcg gagacgtgga
    23161 tcatgccgtc aatgccctgc tccagctcca caaacgcccc gtaggtcgtg aagttgcgga
    23221 ccttgccgct gacgcgggcg ccaatcggat atttgtcctg cacttcgtcc cacggattgg
    23281 attccgtctg gcggatgccc agagagattt tcttgtcctc ggcgctgacg ctgaggatga
    23341 tggcctccac gatgtcgccg acggccagct cgtcggaagc tttcgcgacg cgcttggtcc
    23401 aggagatttc ggagacatgc accaggccct cgacgccctg ttcgatttcc acaaacgcgc
    23461 cgtagggcat gatgttgacc acagggcccg tgatgcgggt tcccaccgga taacgctggg
    23521 cgatgccgtc ccacggattc ggggtcttct gcttgagccc gagggagatt ctctcttttt
    23581 ccatgttcac gtcgagaatc agcacttcga tttcctgccc gacggagagc atttcggacg
    23641 gatgattgat ccggccccag ctcatgtcgg tgatgtgcag caggccgtcc atgccgttaa
    23701 ggtcaatgaa cgcgccgaag tcggtgatgt tcttaaccac gccgacgcgg gactgcccga
    23761 tcttgatgtc ggccagcagg tctttccgct ggctgcggcg ctggtcttcc aggagctcgc
    23821 ggcgggaaag cacgatattg cggcgctctt tgttgatctt gaggatcttg aactgcaggg
    23881 tctggcccag cagctcatcc gcgttgcgca cgggaacgat gtcgagctgg gagccgggca
    23941 ggaacgcctc gacgcccaca tccacgatca tgccgccctt gacgcggtgc accagcttgc
    24001 cgtcaatgat gccgccctct tcaaagcttg agaaaacgcg gtcccagttg cgctgctgtt
    24061 cggcgagctg aacgctgagg accaccattc cggagtcgtc ttccagcttc tgaagcagca
    24121 cttcaatggt gtcgccgacc ttgatggtct cgggctcgcg gaattcagaa atgggcacga
    24181 ggccctcgga tttgtatccg atgtcaatca gcacggcatt gtcgcggatt tcaataattt
    24241 taccgggaat gatggagcct tccgagaagc ttttgagggt ctcctcgtac atggcggcca
    24301 tgggatcctg cggggcggcg gcggagtact tgtccgcggc ctttttggcg ggtttggttt
    24361 ttgtcattaa tattttttgt taaacaccgg gctgtaatca gtacaaatcc ttccggcagc
    24421 agcccgacca ccgccggaag gaagtcgtgt tggtatcaca cgcggtccgg aaaaagcaag
    24481 cgctatcggt aaaaaaagag caggccccgg agcgaatatt cagaacacgg gcttttcaaa
    24541 agaaggcggc cgggttatga tggtcatcgc agaaatggag cggttatgag tcttctgata
    24601 cgaaacggcg agattgtcac accggattcg cggtactgcg cagacatctt ctgcgagggc
    24661 gaaacgatca cgcgcatcgg ccgcgggctg gaggccccgc cggaggccga agtcatcgat
    24721 gcgcagggga aatatgtttt ccccggcttc attgatccgc atgtgcacgt ttatctcccc
    24781 ttctccggca ccttcaccag ggacacctat caaacggcca gccgggccgc gctgctgggc
    24841 gggacgacct gcttcattga tttcatcagc ccgacaaaca aagaagacct gctcgaaagc
    24901 gtttttatct ggcacgggaa ggccgccggg cagtcagcct gcgactacac ctttcacatg
    24961 actgtgccgc gctgggacag cggggcggaa tcgcagcttc gggaaattgt ggcgtcgggg
    25021 attccctcct tcaaggttta cctcgcctac aaaagctcaa ccgggcttga cgacccggga
    25081 ctttttcaca cgctgaaact ggcgaaggaa ctcggcgtgg tggtggcctc gcactgcgag
    25141 aacgcgacca tcgtggagga actgcagcgg cagttcatcc gcagcggaca gaccggtccg
    25201 gaatggcact actggacgcg tccgccgctg gtcgaggccg aaggcgtgaa tcatctgctg
    25261 accttcgcgg aactgcacgg cacgcacacc tacatcgtgc acaccagctg ccgggaagcc
    25321 ctcgaggccg cggtgcgcgc ccggcggcgc ggagtgcaga cgtggatcga aacctgcatt
    25381 cactacctcc tgctcgacaa aacccgcgcc gaacagggcg gttttgaagc cgcgaaatac
    25441 gtcatatcgc cgccgctgcg cgacaaagac cagcaggcgg atctgtggct cgcgctgcgg
    25501 gacggcgtga tcagcacgct ggccacagat catgcgccca ccgatttcgc gacgcagaaa
    25561 gctctcggga aggatgattt cacaaagatc accaacgggg tcccgggcat tgaagaccgc
    25621 atcaacctgc tttacaccta tggcgtctgc ccgggcaaaa tcgatctgca tactttcgtg
    25681 gatgtcgcca gcacgcagcc cgcgaagatt tttgggatgt tcccgcggaa gggcgcgatc
    25741 cagccgggcg gcgatgcgga tctggtgatc tatgatccga acttcaaagg gaccatctcg
    25801 gccaagatgc acagcatgaa tgtggactac aacgcctatg aggggcgcga gatcctcggc
    25861 cggccggaca ccgtcaccgt gcgcggccag gtggccgtcc gcaacggcgt tttcaccgga
    25921 tcctgcacgc acggccgctt cgtgaaacgc gacccatcgc atttctgacc atgcctgttt
    25981 ttgcgccttg attgattgtg ccgattgcga cacaattcgc cttcagttta agtataggag
    26041 ataaattatg aacagcacat ccgctccttc atggacaaaa ctgcttctgg tgctcagctt
    26101 tgccgccctg gcctgcacgg gcatccggac ggtctccaat gacgccgtct ggacgcatct
    26161 cgccgcggga aaggccgggc tggtgaagac agacaccctg tccttcacgc gggaaggcga
    26221 agactggatc aatgcgacat ggctctacga ccgcattcta aatgccgtat ggagtgactc
    26281 caacccgtgg ctggccgtgc tgattcatgt ggccctggtt atcgcggcgt ttctgctgct
    26341 gctgcccgcg gcgcgcgaat ggggcggtcc tgtatccatt tcactctcgc tcctgctgag
    26401 cacatggctg ctggccccgc gctttgaagt cagtccggcg ctggcgggtg tattctttgc
    26461 cgcgctgttc atcgcgctgc tggaaaaaga acgcagcctg tgggtgctgc cggcggccct
    26521 cataccggcg cagattctgt ggaccaacat gcaggaggga ttcctgatcg ggccggcgct
    26581 gtgcatggtc tataccgttg aagccttcca gtttgatgcg gccacccgcg ccgcgcgcgg
    26641 gaagaatccc ccggttgttc ttgccgccat gacagtaatc ctgctggccg ccacgctgat
    26701 caatccttcc ggcctgcggc ttcatggcgc ggtcattgcc tcctggacca gcgccgccgc
    26761 cgcctttacc ggcacgtgga tttcgccgtt caacgccccg gcgcagtcca ttacaaggca
    26821 tctggtcacg ctggcgatgc tgaccggcgc cgcgggcctg ctgatgcagc gcaagaaact
    26881 tcccacggcg ctcacaaccg ccgccgtgtt cagcgcattt gtccttattt tcttccgcct
    26941 caagctggac tcgttcatct ggttcgccct gctggccttc cctttcttct gcctgagcct
    27001 gagcgcagtc ggggaaagct tcggaggttt tcttgagcgc cggaaaaacg cgaatcccgc
    27061 agcgctgcag gcccgcacgg gcatcgtggc gctcctgctg gtccttttca cgatcttcca
    27121 gttcattaca aaccgctttt acaatgccga gcagagctgc tcccgcttcg ggtttggagt
    27181 ggagcaggat gtgttcccgt cccaggcgct ggcggtgatc ggacgcgcag gcttcccggc
    27241 aaaggccgtg aacatggtgc gcgacggagc ctatctggcc tatcatctgc cggagcgaaa
    27301 ggttttcacc gacggccgcg gagatctcta cggcgccgcg ttctatcagg cgctcaacct
    27361 cgccctgcgg ggagacgcgg aacagtggga ggccctgctg gccaaatggg accccgaggc
    27421 tctgatcctg aatatgtgcg acccttccgc cagctccatc acgcatcatc tgctgatgac
    27481 cgggaagtgg atgctggcct attttgacgg aaccaccgcc atttttgcca cgccggcatc
    27541 gggactgcat gaaaaaatcg cggaagaagg gcttcaggcc gaagggctga aaagcataga
    27601 ccgggcattt gccggcgacg aaaacggagg cggcctgatc cgccggcccg tttcggcccg
    27661 gctggcgggc gccggacagc tgttcctgaa catgcgccgg tatccgcagg ccgaagccgt
    27721 gtatgagaga ctcacgaaga gcgtgccgga ttttgcgcag gcctggtaca atctgggaat
    27781 cagccagctc agtcaggagc acgcagacaa ggccgcaaag agttttgacc gcgtggtccg
    27841 gcttcagcgc aaaaacgcgc tggcctggct gtggctcagc ctcgcgcatg ccgtgctcgg
    27901 gaaccaggcc gaggcccgct atgccttcac aaaaggcaag cgcctcaaca gcacgctcgc
    27961 ggcatccttc ggcgatccgc tggcgaccgg ggacagcacc gagaagccct gaccggcgcc
    28021 atgcttgaga cgggagccat tggaccccgg cagcctgccg aacatcttga gggacccgtt
    28081 gaacgggtaa ccttccacag cgaggaaacc ggtttcgcgg tcctgaaggt gaaagccaaa
    28141 ggacatcgcg gtctggtcac cgtggtcgga cagcttccca atgtggttcc gggcgagatc
    28201 gtgcaggccc aggggacctg gctccacgac cagaagcacg gccggcagtt caaatccgaa
    28261 tccatccgca cggtccctcc tgaatccatt gatggcatta cccggtttct cgggtccggc
    28321 ctgatcaggg gcattggacc cgtctatgcc ggcaaactgg tggaagcttt cgggaaggac
    28381 atcttcgacg tcatagaaaa ccggtcggcg ctcctggaac gggtcgaggg catcggcagg
    28441 atgcgcaggc tgcgcatcaa ggagagctgg ggagaaaccc gctcggtgcg cgccatcatg
    28501 agttttctga tgagctgcgg ggtcagcacc gcccgcgcct tccgcatcta ccgcgtctac
    28561 ggcgatcagg ccatccagca ggtcaagaag gatccctact gccttgcgcg cgacatccac
    28621 ggcatcgggt ttctgaccgc cgacaagatc gccgaaaaaa tggggatagc ccgcgacagc
    28681 gagctgcgcg cgcgggccgg cgtcgagtat gcgctcatgg aactgacgca gcagggacac
    28741 tgcgcctgtc cgcgggagca gcttcaggag gaaaccgcca gactgctgca gattgacgag
    28801 tgcctcgcgg gcgaggccat tgaccatggc cttgcaaaag gccgtcttgt ggccgggaat
    28861 caggattaca accgcgaact tgtctatctc gccagccttc attgttccga aacagctctg
    28921 gcggcgcatc tttccgcgct ggccgcaggc cctcacccct gccccggcat tcttgtggac
    28981 aaggccacag catgggccga ggaaaagaca aagatcagcc tgtccgacga tcagcggcgc
    29041 gcgctcgccg ccgcgatcac cgccaaggtc ctggtgatca ccggcgggcc gggcgttgga
    29101 aagaccaccc tcatcaatgc cgtgatcagg attctgtcgg caaagaaaat gcaggtggtg
    29161 ctgtgcgccc ccaccgggcg cgcggcgaag cgcatgacgg aaagcacccg acatccggcc
    29221 aaaaccattc accgtctgct ggcctatgat ccgaagtccc gccgtttccg gttcaacgat
    29281 aaaaacccgc tcgcgggcga catcttcatc gtggatgaag ccagcatgat agatcttccc
    29341 ctggccgcgc agctgatcag cgccattccc ggccacgctt cgctgatcct tgtgggcgac
    29401 gtggaccagc tcccgtcggt cggccccgga aatgttctga aggatatcat tgcgtccgga
    29461 atgtttcccg tccaccgcct gacacagata ttccggcagg ccacgcacag cgaaattgtg
    29521 ctcaatgccc atcgcatcaa ccaggggctc cccgccgaac ccggcgagcc cggaagcgag
    29581 tccgattttc agttcattga acgggatgat cccgagcagg tgctcaagac actggtcggg
    29641 ctggtggaaa cctccattcc aaaccgctac cggctggacc gcctcagcga catccagatc
    29701 ctgacgccca tgaagcgagg gctgctcggc gcggatcacc taaaccgccg gctgcagcag
    29761 gcgctcaatc cccggccgga aggcatagag cgctttggag ttcgctactg cgtcggcgac
    29821 aaggtcatgc agcagcagaa caactatgac aaggatgttt tcaacggcga ctgcggagtc
    29881 atttcggcga tagacgaagg cgcacgagag atgatcgtga atttcgacgg ccggcccgtc
    29941 agctatgacc tgcaggagct ggacgagctg ctgccgtcct atgcgatcac cattcacaaa
    30001 agccagggaa gcgaatatcc ttgtgtggtg attccggttc acacgcagca ctacatcatg
    30061 cttcagagga acctgctcta caccgcgatc acgcgcggga aaaagctggt gattctgatc
    30121 ggatcgagaa aggcgctggc catcgccacg cgcacggcgg aggccgggcg ccgtgtcacg
    30181 acactggcgc agcggctcca gcaccgggat cagcctttct gacagccggc ggcagttttt
    30241 ccgctggcag agctgtctga aagagcatag tatgctttgc ggttcttgta aggcgcaacc
    30301 ggcgcggttc atcatgggca gaattaagat cagaaaggcg tttcagcagg ccgtgcggcc
    30361 tcttcttctc ggcatgcgga gaatgacccg cgaggagatc ctggtgctcg gagactccca
    30421 tgcgacggtt tttgaacacc ggaatttcaa aaaggctttc ccgcggcatt tcttcaatgt
    30481 tgtatccgta cggggcgcaa cggtgtcggg cctttcaaat ccgatgtcaa aaacccaggc
    30541 catgcccatc ttcatgaaag cgctgaagcg atcaaacgcc aaaacctcca tcgtgctgct
    30601 gggcgaagtg gatacaggat tcctgctctg gtacaaggca cagaagcaca gcgcgccggt
    30661 cgaaatcatg atggaccggg cgatcgaaaa ttatcagaac ctgctgcgca ctgtggccgc
    30721 gaaatcaaaa gttgtctgca tcagcgcccc gctccccacc atccgggatg atcaggatct
    30781 gggcgaaatc gccaacctca gaaaggaagt caaggccaca cagcggcagc gcaccgaact
    30841 cacgctcgag ttcaaccggc gcatgcaggt tttctgcgaa gaaaacggtc tgcggcatgt
    30901 ctcgctggac cgggaggctc tggggccgga tggccttgtc agtgaacacc ttctgaacac
    30961 caaccccagg gaacatcact acgaccggga gaagcatgcc ggcctgatca tccggcacct
    31021 cttgaactgc gccgaattca gcggcgggtc tgaaacatca gtccggggac aatgattcgg
    31081 acttctgtcc cgcggcggca tagcgcgaag cgacctccgg ccagttcacg acattccaga
    31141 aggcctcaat ataactggcc cgcagattct gatatttcag gtaataggca tgctcccaca
    31201 catcaagtcc caggatcggg gttccccgcc ggtccgccgc cttcatcagc ggattgtcct
    31261 gattgggcgt tgatatcaca aagagcgatc cgtcttccgc aacgcagagc caggcccatc
    31321 cgctgccgaa acgggtggcg gccgcctcgt tgaacttctt ctgaaattct gcgaaagatc
    31381 cgaaatccct gatgatttgt tccgcaaggg caccctcggg ttctccgccg ccttccggag
    31441 acatgacctg ccagaacatc gtgtggttcc agtgcccgcc gccgttgttg cgcaccgccg
    31501 ccgggagcgc gtcggcggag gccagaatat cctcaagcga ccggccggcc atctctgtgc
    31561 cctcgaccgc cttcagcaaa ttgttgaaat aggcctgatg atgacgggtg tagtgaattt
    31621 ccatcgttcg cgcatctatg tgcggctcga gggcatcaaa ggcatagggc agcggcttga
    31681 actccagcgg ttcacccgcc gccgcggttc cggccgccat catgatcacc agaaatgcct
    31741 gtgctgtctt cttgagtttg cttttcatgt tttctccttt tttaatcgct gaatatatca
    31801 cctttcgcgt ttttttcaaa cgtgccccgg ggctgatgtt tgggattgca atggcgggcg
    31861 gccgttcgca ctatcacagg accttcagga gactgagatg aagtacaatt ttaatgagtg
    31921 cccggaccgg cggccgacag aatcggaaaa gtggctgcaa taccctgaag atgttcttcc
    31981 gctgtgggtg gccgacatgg attttgtttc gccggagccg gtgatccgcg cgctgcacga
    32041 acgcgtggac cacggcgtgt ttggctatcc ccgaagccgc gctgacctga aggaactgat
    32101 cgtcggccgc atgcaggagc gctacggctg gtgcattcat gcggaagatg tgctcctgat
    32161 cccaggcgtc atccgcggat tcaacatggc ctgccgcgcc gccggaccag atggcgaagc
    32221 gctggtgcaa accccgattt acccgcccat gctttgcgcg cctggaaacg ccggccttgc
    32281 gcgcagagat aatgagcttc ctgcatccga ctgctccaat gaaatcgact gggcggattt
    32341 tgaggccgcc cttcgcggcg acgtccgtgt gtttctgctc tgcaacccgc acaatcccac
    32401 cggtcgggtg ttccgggagg aagagctttt gagaatgggt gagctgtgcc tgaagcatga
    32461 tgtcatcatc tgctcggatg agattcactg cgacctgatc tattccggcc agacccacat
    32521 tcccatcgca tcgctctccg cagagctggc cgcacagacc atcacgctga tgtcgccaag
    32581 caagacctac aacatcgcgg ggctggattg ttcgtttgcg atcattcaga atcccgaact
    32641 gcgccggcgc ttcaacaggg cccgcgcggg cctcgtcgga ggagtcaaca tcttcggtca
    32701 ggcggccgcc gcggcggcgc tgaaagacgg gcaggagtgg cttgatcagg tgctggcggt
    32761 gctggaatca aatcgcaact ttctgttcga atatgtcggg cgggaatggc cgggagtgaa
    32821 aatgtttctg ccggaggcca cctatctggc gtggctggac tgccgcgggc tgaaccttga
    32881 aaacccgtat gagttcttcc tgagccgcgc cagagtggcg ttcaacgacg gcgcctcgtt
    32941 cgggaagggc ggcgaaggat tcgtgcgcgt gaacttcgga tgcccccgca aaacactgga
    33001 agaggccctc gcccgctgca ccgcggccct ccgcgaagcc gggctgattc cccggaaatc
    33061 atgatcttcc gtgcggcgga ccgggccttc tgatcccgag ctttctgaat ctcagcagca
    33121 ccccttcgga aacaggttcc cgcaggcggg ccgggagttt tgatatctgg agccggaatt
    33181 ctgaatccgc cgaaacgcct gaagtctcca gcgccttcat ggcgtacagc agcgccccca
    33241 tgcagtgatc cgcgaaatgc gcggtcgcca cggcctgtcc cgccgcgcgg gccgccgcga
    33301 cggccgcttt atcgcccgcc ccgcgcgccg cggcatgcgc ggccagagag gccttcatgg
    33361 cggcgcccgt cttcacctct ccattcgccc acctgcgggc tgtgtccaga gcggcccgcg
    33421 gacgctcgtc atcactgctt ctggtgaaca gatgcagcac gccttcggcg caatccgcgg
    33481 cccaccgggc aaggagaaca tgatcctccc gactcaaagg ccccccgcga tgcgcggcaa
    33541 taaaacgttt gtccctcatc gaaacatcct cgcagttcct ttccccgcaa tttacccctg
    33601 atccactcag caataaaaga gcaaagaccg aaattgattg aatgattaaa gcccggcggc
    33661 cgttgtaaaa aatagtacta aatcagtcct attttgcgga ttaaaacttc caatgactgg
    33721 aacttttcac gcaaaaactt ccaatgattg gaagttttca gacagaaagt tccagtcatt
    33781 ggaagttttt aaccggatga agaggcgctc acccttcctg catttccttt tcctgccgga
    33841 cgctgcgggg gaagaggatg ttgttttcga ggtggatgtg ctcgtgcaga tccgcctcga
    33901 tggcctgcag gccgtcgtag agggccgcga aggtttggca ggcgtcttcg gggagtgtgt
    33961 agtttccggt gatccggcgc atctccgaca gcgcctcgcc ggcgctgtcg tgctcgtatt
    34021 ccatctggcg gatgggattg gccacgctcc cgcagtgaat ttccggaacc ccgcccgtgc
    34081 ccgacacata ggcatccacc gccttgatgg ccggaaacag gatctgttcc tctttcatca
    34141 ggtgcatttc aatttcagca cgcagcgagt caaacacccg gcgcaagtcg tcaatcatgg
    34201 gcccgtgatt ttctccgtgc gcgctgcgca ctttggtcag aagactgtcc atgcgcggga
    34261 gctgctcttt catgaacgtg tgatgcctgt tcagaatatg atccgccagc tcggtgacgg
    34321 gaaccttgtt ccagtccttc tgcgcggacg attttgcttc tgcctgcagt ttgctgacaa
    34381 aggcaggcca ttcgatgccg gccgccctga cggcttcacc cagagggcgt ttgccgccgc
    34441 agcagtagtc tatgcccatt ttctccagct tctccttcag ctccggatgc tcaacaacca
    34501 gttcggccac ggtctggtcc tcgcgaaccg aaattccgct ggaggacatt tccgacggat
    34561 tgggggtttt caggacgatg aaggaagcat tggaatccgt atcgtttctg atggtcatgg
    34621 gcgtctggaa cgcaaccggg agcagcgtcc cgcgcggggc cggaaattcc ctgccgtcaa
    34681 gcgtcacgat gacatcgccc tcaagcacca gcagatgaac attcgagttg gccttgtgga
    34741 gaggaacctg ctggccgggc ttcagggcaa tctgcatgac cagcaggggc ttttcattca
    34801 ccagctgccg gctgcccatg cgtccttccg tatagggaac ttccttaaaa atatttttca
    34861 tcatatcttc tcccgcgaca cggccgcaaa tcacggccac tgcaatagtg gcggcaatat
    34921 acttcatatc tgtcatattt cacagacctt caaacagaac tgtggacaaa tatcgttcgc
    34981 cggcgtccgg cagtatggcc acgatggttt tgcccgcgaa ttcttcactg gccgcgagac
    35041 gcgcggcgac cgcggccgcg gcgccgctgg atatgccggt cagcagtcct tcctcgcgcg
    35101 caagccggcg cgccatggtg atggcttctg ccgagctcac cttttcaacg cggtccacaa
    35161 ggctcaggtc caggttttcc ggaatgaacc cggcgccgag gccctgaatt ttatgcgggg
    35221 ccggcttgag ttcctgtccc gcaagtttct gaaagatcac cgggctttct tcaggctcga
    35281 ccgcaacgga aagaatcttt ttcccttttg tttttttgat aaagcgcgaa acacccgtga
    35341 tggttccccc cgttccaacg ccggacacca gcacatcaat ggccccgtcg gtgtcgttcc
    35401 atatttccgg tccggtggtg ctttcgtgaa tggccgggtt ggcggggttc ttgaactgct
    35461 ggggcatgaa gaacttcttc gggtcggatg cggcgatgga ctcggcacgg ctgatcgcgc
    35521 ccttcatgcc ctcggcaccg ggagtcagca tcaggttggc cccaagcgcg gccagcaccc
//
LOCUS       DAOQWR010000007        35372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1409_length_35372_cov_5.624827, whole genome shotgun sequence.
ACCESSION   DAOQWR010000007 DAOQWR010000000
VERSION     DAOQWR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 35372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35372
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1409_length_35372_cov_5.624827"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="rplP"
                     /locus_tag="PKK36_01135"
     CDS             <1..214
                     /gene="rplP"
                     /locus_tag="PKK36_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNR93193.1"
                     /translation="FPDKPVTAKPLEVRMGKGKGAVEEWVAVIRPGCMLYELDGVTES
                     VAREALRLAAAKLPIRTRFVQRNVHA"
     gene            228..425
                     /gene="rpmC"
                     /locus_tag="PKK36_01140"
     CDS             228..425
                     /gene="rpmC"
                     /locus_tag="PKK36_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNR93194.1"
                     /translation="MKASEIRELTGEELAQRIDDARKELFNLRLQQTGGQLEKPSRIR
                     EVRRDIARMSTEARHRQKVSR"
     gene            431..700
                     /gene="rpsQ"
                     /locus_tag="PKK36_01145"
     CDS             431..700
                     /gene="rpsQ"
                     /locus_tag="PKK36_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNR93195.1"
                     /translation="MTEAARNLRKTRTGIVTSDAMDKTIVVEVGRRMSHPLYGKVVNR
                     KKKYHAHDEKNEAHKGDQVRIAETRPLSRLKRWRLVEIIKKAAAE"
     gene            714..1079
                     /gene="rplN"
                     /locus_tag="PKK36_01150"
     CDS             714..1079
                     /gene="rplN"
                     /locus_tag="PKK36_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNR93196.1"
                     /translation="MISVETNLDVADNTGARSVRCIRVLGQRKTTAGVGDIIRVAVKE
                     AQPKGMVKKGEVHLALIVRTKHPIRRPDGSLLRFDHNAVVIIDDQKNPRGTRIFGPVA
                     RELREKNFMKVISLAPEVL"
     gene            1079..1363
                     /locus_tag="PKK36_01155"
     CDS             1079..1363
                     /locus_tag="PKK36_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNR93197.1"
                     /translation="MKKKKQVFSAKPQIKKGDMVEVLAGDDAGKTGKVLQVIPQKGAA
                     IVEGLNIIRKCVRKTQDNPQGGIVEREAPLALSKLRAAKSAGKKAAGEKK"
     gene            1408..1950
                     /gene="rplE"
                     /locus_tag="PKK36_01160"
     CDS             1408..1950
                     /gene="rplE"
                     /locus_tag="PKK36_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNR93198.1"
                     /translation="MTPRLKEKYKKEVEPALRKQLGCKNPMQVPRFQKIVINVGTKAT
                     LDKDSLKEIAENLAKITGQKAVVTKSRKSIANFKLRDGMPIGVKVTLRGDRMFEFMDR
                     LVNVALPRIRDFRGISATGFDKSGNYTLGLKEHDLFPEIDPDRVKVAHGMDITIVNTA
                     ANRTEALALLKLMGFPFAGE"
     gene            1975..2160
                     /locus_tag="PKK36_01165"
     CDS             1975..2160
                     /locus_tag="PKK36_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNR93199.1"
                     /translation="MAKKSLVVKSNRAPKFSARKYNRCRNCGRPRAYIRKFQLCRICF
                     RELASQGKLPGVTKASW"
     gene            2171..2569
                     /gene="rpsH"
                     /locus_tag="PKK36_01170"
     CDS             2171..2569
                     /gene="rpsH"
                     /locus_tag="PKK36_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNR93200.1"
                     /translation="MSLSDPIADMLTRIRNASAAGAKSVEIPHSRMKEGLARILKQEG
                     YISDYTVSGEGARKGLRIRLKYVREGDPVITGLRRVSRPSLRRYVAATEIPRVLGGMG
                     IAVISTSSGVMTGHEARKKNVGGELLCTIW"
     gene            2591..3127
                     /gene="rplF"
                     /locus_tag="PKK36_01175"
     CDS             2591..3127
                     /gene="rplF"
                     /locus_tag="PKK36_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNR93201.1"
                     /translation="MSRVGKNPVELPAGVTAEVGGNTVKIKGPKGELSLDVAPGISVE
                     VKDGRVLVSRAADDKQSRALHGTVRSLVANMVKGVTQGYRKELEIYGVGYKARMEGRK
                     LVLNLGYSHPIEYMIPEGVKITVPKPEDVGIEGVDKHLVGQVADRIRSFAPAEPYKGK
                     GVRYKGEKIRRKAGKTVA"
     gene            3130..3489
                     /gene="rplR"
                     /locus_tag="PKK36_01180"
     CDS             3130..3489
                     /gene="rplR"
                     /locus_tag="PKK36_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019737210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNR93202.1"
                     /translation="MKVKTKKEYIGRRHARLRKKIRGTAERPRMCVKVTSRHIYVQMI
                     DDDAGCTLAAASSMVKDLKSLAAKKTELAKQVGKAAAEAARAAGITQVVFDRGGFTYG
                     GRIKALADAAREAGLQF"
     gene            3518..4000
                     /gene="rpsE"
                     /locus_tag="PKK36_01185"
     CDS             3518..4000
                     /gene="rpsE"
                     /locus_tag="PKK36_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR93203.1"
                     /translation="MISEKTEGHEVEERVVHINRCSKVVKGGRRFSFSALVVAGDHGG
                     RVGYALGKANEVSDAIRKGGELARRNMFRVKMLNERTIPHEVTGSYAGGVVMLKPASA
                     GTGVIAGGAVRAVLDLAGIRDVLAKSMGSNNQLNVTKATVDALRQLRTREEVQSIRRG
                     "
     gene            4023..4469
                     /gene="rplO"
                     /locus_tag="PKK36_01190"
     CDS             4023..4469
                     /gene="rplO"
                     /locus_tag="PKK36_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNR93204.1"
                     /translation="MKLHTLKPSRGAKRSKTRLGRGRASGKGKTSGHGHKGQMARSGA
                     NHRVAFEGGQMPLYRRLPKRGFNHEKAVVLVPVNVSSLNRFDAGTEVTFELLQQSGLA
                     RGGKADGVKILGNGTLEKKLVVKAQAFSASAVEKIKAAGGECTLVS"
     gene            4490..5842
                     /gene="secY"
                     /locus_tag="PKK36_01195"
     CDS             4490..5842
                     /gene="secY"
                     /locus_tag="PKK36_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR93205.1"
                     /translation="MLSAFSNSLKIPELRARIFFTLGLIFICRLLARVPTPGVDAVAL
                     RDLIASLQTQAGGSMLVLFNLFSGGALQYAAVGALGIMPYISASIILQLMTAVVPALE
                     RLAREGEAGRQTLSQYTRYLTVALCIIQGYAMSATLWRPEALGLPAGTQIVTMGRMGF
                     TLLTILTMTTGTMMLMWLGEQMTERGIGNGISLIITINIISDLPSAVQAIVRMFVRVD
                     GVAQFTIFHLVGLLAMIFLVIAGTIAVTQAQRKIPVQYAKRVVGRKVYGGQSTYMPLR
                     VNYSGVMPIIFAQAILMFPSKLLGMLPGEFFRRLAAAVYYGTPAYNVIYALMILFFSY
                     FWVATQFNPVQIADDLKKYGGYVPGIRPGKPTAEFLDRTMTRITLAGAVFLTIIAVLP
                     TIMAQQFNIPWIVASFFGGTSLLIMVGVMLDTMRQVESHLLMRHYDGFLKKGKLRSRR
                     "
     gene            5854..6504
                     /locus_tag="PKK36_01200"
     CDS             5854..6504
                     /locus_tag="PKK36_01200"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNR93206.1"
                     /translation="MDAIILLGAPGAGKGTIADLVKEKSGYLHVSTGDMLRAAVKAQT
                     AVGLKAKSFMEAGELVPDEVIIGIVRERMAQGGQDEKYMFDGFPRTLEQARLLDAVME
                     EAGSRVSRVFLLEASKELLVSRISGRRICRNCGAVYHVQNIPPKVEGVCDSCGGELYQ
                     RKDDSEETVLNRLEVYKKQTAALIDYYDAKSILLRLDASQPKEVTQARILEVLSRS"
     gene            6515..7273
                     /gene="map"
                     /locus_tag="PKK36_01205"
     CDS             6515..7273
                     /gene="map"
                     /locus_tag="PKK36_01205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNR93207.1"
                     /translation="MIRIKTETELEKMRVSNRMTAEVLAAVAGRVAPGITTGELDRFA
                     GEMIRDMGGTPAFLGYGGYPASICVSINEQVVHGIPGRRVISSGDVVSIDVGVKYDGF
                     IGDCATTVLVDVDDREIIRLAAVTRAALAAGIDKAVVGGRLSDISHAVEKTVTAAGFS
                     VVRDFVGHGIGRDMHEEPQVPNFGRPGRGPKLAHGMTIAIEPMVNQGGCEVEVMPDGW
                     TVLTRDRRPSVHFEHTIAVLKTGPEILTCVKKKI"
     gene            7463..7576
                     /gene="rpmJ"
                     /locus_tag="PKK36_01210"
     CDS             7463..7576
                     /gene="rpmJ"
                     /locus_tag="PKK36_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNR93208.1"
                     /translation="MKVRSSVKRICEQCKIVRRKGVVRVVCTNLRHKQRQG"
     gene            7589..7987
                     /gene="rpsM"
                     /locus_tag="PKK36_01215"
     CDS             7589..7987
                     /gene="rpsM"
                     /locus_tag="PKK36_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNR93209.1"
                     /translation="MPRLLGIDIPGKKRVDVSLRYIYGIGPKRALEVAAKAGIDPAKK
                     ADDLTSDEISKIAGILQSEYRIEGDLRREVSQNIRRLMSVGAYRGIRHRRSLPVRGQR
                     TSTNARTRKGPKKTVGAVRGKEARSAVKAG"
     gene            8016..8492
                     /gene="rpsK"
                     /locus_tag="PKK36_01220"
     CDS             8016..8492
                     /gene="rpsK"
                     /locus_tag="PKK36_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNR93210.1"
                     /translation="MAEEIQKEQAETAEQAAAEGSVQAEGAEGEQPKVVRRKVKGMKN
                     VPLGIAHIRATFNNTIVSISDMQGRVISWSSSGRCGFKGSRKSTAFAATTVAQEAARV
                     AVGFGMHEVEVRVQGPGAGRESAIRALQAAGLTVTSIKDVTPIPHNGCRPPKRRRV"
     gene            8521..9147
                     /gene="rpsD"
                     /locus_tag="PKK36_01225"
     CDS             8521..9147
                     /gene="rpsD"
                     /locus_tag="PKK36_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNR93211.1"
                     /translation="MGRYTGPACKQCRREQMKLFLKGERCFMAKCPIETGRPQPGGAG
                     RRRKKTSDYGNQLREKQRLRRMFGLREGQFHNFFERAAKKRGITGETLLQMLELRVDN
                     LVYRLGFAPSRRAARQFVRHGHVRVNGERANIPSMILKPGDSITVKDAKDSREYASKH
                     MDQAEARGIVPWMSLDKNNFKGEVLHVPTRDEMAPLVDEQLVVELYSK"
     gene            9205..10218
                     /locus_tag="PKK36_01230"
     CDS             9205..10218
                     /locus_tag="PKK36_01230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNR93212.1"
                     /translation="MPIRLGRFEMPKRIEPDDSTATDTYKKFVAEPFEAGYGRTIGNS
                     LRRVLLSSLEGAAISSVKIDGAMHEFCTLKGVVEDVTDIILNLKQVLLRMYSREIRKV
                     RIDVKGPGEVTAGDIKTDGHVEILNPDMHICTLDRDGHFKAEFEIRIGRGYCPAEWNK
                     KEDQEIGVIPIDSLFSPVRRVKYYVENTRVGQRTDYDKLILEIWTDSRITPDDALTQS
                     AAILLHHLDVFVSYDKDLIEFEESEKQIDAEKEDLRKKLNISVNEIELSVRAANCLNN
                     ANITTVGELAQKTEAEMLKYRNFGKKSLNEIKNKLTELGLSLGMTFDPELLKPVAPSA
                     EDN"
     gene            10237..10773
                     /gene="rplQ"
                     /locus_tag="PKK36_01235"
     CDS             10237..10773
                     /gene="rplQ"
                     /locus_tag="PKK36_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNR93213.1"
                     /translation="MRHQKRTIKLGRTSSHRDALLASLVCALIRRKRIRTTLQKAKAA
                     RITAEKMVTLGKKGTLAARRQAISKLRDTECAALLFAEIAPSFKDRQGGYTRIIKLGQ
                     RPGDNSQVAILEWVMEHSVQATKKKKSAAKKEAEAAEKAPEKKAPAKKKAASKAKKEE
                     TSEKAPARKKTSRKKKEE"
     gene            complement(10829..11473)
                     /locus_tag="PKK36_01240"
     CDS             complement(10829..11473)
                     /locus_tag="PKK36_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93214.1"
                     /translation="MRTITVVFSVMALAAAVQARPPASARINIREKLGTNKAGLPEPA
                     GVATQPVGPSSSDGAPVNLPSKWLKNAEGYQEALEIQKQTGADIFVFFVRPDVSDEKG
                     LCNWLETRGFQTSPVQKFFKEVIKVRATLPSNRETEAMAEMFRVNKTPAVFMVHPNGK
                     RWRCSTFKYDSKRPELPEPAVLAEQFRSQSSKAMAAAAASAASEAQSPSVPPQQ"
     gene            11520..12485
                     /locus_tag="PKK36_01245"
     CDS             11520..12485
                     /locus_tag="PKK36_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93215.1"
                     /translation="MKEYELLSRIYSALDSCLKCYTPRMGKNVGQGICTLMIFVDGLG
                     LGHSDPEVNPVFSGACPVLCELLREAAAIDAVMGVEGLPQSATGQTSLLTGVNAQAQM
                     GRHIEGFPNRELRDIIRRENIFGKMMERGYSSTFANAYYLQAYTSAELRRRRSVTTVA
                     TEAAFGKFRSLEDMLAGRAVYQDLTREQLRRRGYEGPLIEPEEAGYHLVEIARAHDFT
                     LFEYFQTDLAAHRGDRQALLSVLEKLDRFLGILLHMFRGSNELFLLTSDHGNLEDFGT
                     RLHTGNPVPFAARGREASRLKAKIHSLKDVVPALLELYPPGHSIA"
     gene            12518..13012
                     /locus_tag="PKK36_01250"
     CDS             12518..13012
                     /locus_tag="PKK36_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNR93216.1"
                     /translation="MSKAADTRPDWDTYFMEIAHVAATRSNCSRRHVGAIVVRDKRII
                     TTGYNGTPRGVRNCFDGGCPRCASDSPSGNSLGDCICAHAEENAIVQAAYHGISVKDA
                     AMYVTISPCLMCAKMIINAGIREVVYEGEYRFTESTRSLLQEGGVICRQFTRAAGFNS
                     STGL"
     gene            complement(12990..14690)
                     /locus_tag="PKK36_01255"
     CDS             complement(12990..14690)
                     /locus_tag="PKK36_01255"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNR93217.1"
                     /translation="MKKITVALCQINVAVGAIRANADKMAAAAGEAAARGARLILFPE
                     LALCGYPPEDLVLKPHFIRDCTAALHDLQARLPAGSVTIFGAPWTEQQKVFNGAVVCA
                     GGEVRGVYKKIALPNYGVFDEKRIFVPGERPLILNFGGARIGVHICEDSWLPDEPACR
                     LLAPFQLDGLVNISASPYERGKLSRREAALQKTAVKLDTLLLYCNLVGGQDELVFDGA
                     SMVVSPKGPTCMRGRQFAEETLYFNLPAPRKGAHSKQADADIIDIPVTGAAEITSSPA
                     DSAVAPALGPAEEVYAALKTGLRDYVNKNAFQNCVVALSGGIDSALVAAIAADALGAE
                     RVHGITMPSRFSSEATRADAVQLARNLKIDFRTVAIESLFKAYLNELTGQWPGKAPDI
                     TEENLQARIRANIIMALSNKFGWLVLTTGNKSELAMGYCTLYGDMVGGFAVIKDVPKT
                     LVFELARWRNSQDTVPVIPPSTIERPPSAELRPDQKDSDTLPPYEILDPILELYIEQH
                     CGLEEIMARGFDGDIVRMTVRTVDRNEYKRRQGAPGIKITPCAFGRERRMPITNQYWS
                     "
     gene            14801..15910
                     /locus_tag="PKK36_01260"
     CDS             14801..15910
                     /locus_tag="PKK36_01260"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNR93218.1"
                     /translation="MSDITDDNLEPVPADRMSSTDDQRIENILPLPPPAHLIRFFPIK
                     DTPAEKLIGTTRRRIRSILRGQDDRLLVVIGPCSIHDPASALEYAERLKTEREKRRGE
                     LEILMRVYFEKPRTSIGWKGLINDPYIDGSFKINEGLRIARDLLVRINQLGIPAACEF
                     LDVISPQYIGDLVSWGAIGARTTESQVHRELASGLSAVVGFKNGTDGNIKIAVDAILA
                     ARERHHFLSVHKSGQVAIVDTCGNSDCHVILRGGRVPNFDPKSVEAACSELRAARLPE
                     RIMIDLSHGNSAKDHREQLKVGANVAAQMAGGEMRIMGVIAESNLIEGRQAISAKKRL
                     VYGQSITDPCLGWEDSVQLLGQLADAVRARRKARK"
     gene            complement(15945..17426)
                     /locus_tag="PKK36_01265"
     CDS             complement(15945..17426)
                     /locus_tag="PKK36_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNR93219.1"
                     /translation="MKKNTSPAAHDSPVIKFIVGEQIPVELHKVRIVQKLNLKPVNER
                     LRAIQDAGFNSFLLSTQDVFLDMLTDSGTNAMSDQQVASMLVADDAYAGSQSFERLQK
                     AVKEVFGKKYFLPAHQGRAAEHIIFHAFIRQGDVIPMNYHFTTTKAHIELPGGVIEEI
                     FTSEALKIKSSHPFKGNIDIEKLKKLIKKHGVDRIPFVRMEASTNLIGGQPFSIENMR
                     QVRKVCDQHKIMLVLDASLIGENAYFIKQREAAFKKKSIAEILRTMCDLSDLVYFSSR
                     KVSSTRGGGICTNDVHLYDKLKDLVILFEGFLTYGGMSVREIEAMAVGLRETQDETCI
                     SQSPQFIKYLVEHLDAAGVPVVTPAGGLGCHIDAKGFLPHVPQTEYPAGALTAAFYII
                     SGVRGMERGTISSVRDVSGKDILADMELLRLAMPRRVFTLSQVKYVADRLQWLHKNRK
                     LIGGLQFEYEPAVLRFFTGRLTPTSDWPQKLMAKFKKDFGNSL"
     gene            complement(17484..18728)
                     /locus_tag="PKK36_01270"
     CDS             complement(17484..18728)
                     /locus_tag="PKK36_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNR93220.1"
                     /translation="MMNNEHQEQIRGFTGWLARRVLKSGADLRTGDSRSRLGMLQGAV
                     GIIANLFIFLVKITFGFVFGSIALIADSFDSLSDVAASTMIIVGAHWAGKPRDREHPF
                     GHGRMEWIASLIVSILLVGVGLSLGKEAVVRIFRPHPYDAPWWLIAIVAASIPLKEWM
                     AHFSRSLSRASGSSLIEAGYWHFRFDSLMAGLVCVALFSSRFGWAAVDGWMGLIIAGL
                     IMHGGLSLIRERVSLLLGEAPSAEEISQLEKLAFQVDGVRDAHDIIVHKYGDYRLISL
                     DIEVDACGTALEAHRIAAQVEQKIEDNCHAKAIVHVDPVDRSHRLYPEAESVLSRYIS
                     AHGEIASFHDLRLTDEEGGPVLTADIVMATKTPETAHAQIIEDLKNHLSLHLHTVSRL
                     HIDTEIFHGQPAASPAVGKINA"
     gene            18843..19181
                     /locus_tag="PKK36_01275"
     CDS             18843..19181
                     /locus_tag="PKK36_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93221.1"
                     /translation="MASRKEEQFEGSFFAKPAVGSLFTVLSALAFLVLCMVLPLVGQA
                     GVATEYARQNFYTFMAVLAVCFVLAVLAVVSKLERRRLDESPLPYSSLGLCAVCILLF
                     IALVSGMLGV"
     gene            19263..20582
                     /locus_tag="PKK36_01280"
     CDS             19263..20582
                     /locus_tag="PKK36_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR93222.1"
                     /translation="MNSMKIWVLTALMAVPAVFAPAVSAAEYDLFAQAPWSVSYEIGF
                     YNFEGDEEVEDAFFHALRLGYDFNPRWTFELDFNLLPKLEPTDFQDPRRSDIDDDTWA
                     IRLGLDVLFHLRNIENLRWDPYLSLGAGWFHYGTSDAQEQDEPIIIGGLGMFYHFNDE
                     WAVRMDVRPVVQGTDTEGNILFSAGVNWRWGAHVPRVYELEGTDIDSDNDGLLDPREA
                     EIGTDPYNPDTDGDGLTDGQEVLSYSTDPLNPDSDWDGLKDGAEVLTYRTDPNDRDTD
                     NGGVADGHEVIEDHTNPLDPADDLQLFTLNIEFDYNKADVRPQYFEQLDVVAKVLQRD
                     PGATAKVEGHADKRPTSKRDYNIRLSERRAKAVMGYLISAGGIEPSRLSSKGFGFDVP
                     VAPNDTEENMQKNRRTEIYIRPSKSAETGGDMDMSQPQEMPEEMPVK"
     gene            20598..20849
                     /locus_tag="PKK36_01285"
     CDS             20598..20849
                     /locus_tag="PKK36_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR93223.1"
                     /translation="MPIYEYTAVDSSKACEYCRKVFEHLAAVKQEPLKTCPQCGSPVK
                     RVISAPNVGGSKSGLDDRAKSAGFHKLKKIGKGEYEKQY"
     gene            complement(21083..21958)
                     /locus_tag="PKK36_01290"
     CDS             complement(21083..21958)
                     /locus_tag="PKK36_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93224.1"
                     /translation="MQGTSKAGSLILAVLLLSGCATTPYHFGAVYDYHPAPPLSEHEQ
                     QIERGKPNRFLDACDWIWPGSLLGKLILWNRKVDSHQVSQETEDTLRSYLAANHLDSV
                     KVRINQYHPGAELSRTLGNKSVGFGWRYTIGLLGWLQYTILPQRFFGGDNYNPYSNTL
                     NIYSDIPAVTVHEGGHSKDFAEKKLKGTYAFIYVIPFVNLWHEAQASNDALSYAQETD
                     HELQRGGYKILYPAYATYIASDISYFTSPVLSYPLMAVCVVPAHIAGRIKAHGVTDEE
                     LSPPETAAAENDTAK"
     gene            complement(21970..22383)
                     /gene="rnk"
                     /locus_tag="PKK36_01295"
     CDS             complement(21970..22383)
                     /gene="rnk"
                     /locus_tag="PKK36_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNR93225.1"
                     /translation="MKNRKISITKFDFARLKELLAVANEFDRTRSDLKALAAELDCGH
                     LVESKDVAATVITMNSRFKIRDLETKEEEEYTLVFPADADFEAGRISVLSPIGTALLG
                     YSEGETVEWPVPAGKRRIKIEKVLYQPEAAGDFHL"
     gene            complement(22404..23261)
                     /gene="sucD"
                     /locus_tag="PKK36_01300"
     CDS             complement(22404..23261)
                     /gene="sucD"
                     /locus_tag="PKK36_01300"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNR93226.1"
                     /translation="MSILINGKSSVMISGLTGREGTYHAKLMMDLGTRIVCGVTPGKG
                     GIEHLGVPVYNTVREAMAKHDIDACGVFVPARVAAAAVEESVEAGVPLIVAVTEGICP
                     HDAMRFISLARRRGVRVLGPNTPGLLTPGEAKIGVLASDFVKKGPVGVVSRSGTLTVE
                     ICYYLQKNGYGQSSVVGIGGDMMVGTTFLDIYKLFEQDGETEKIVVVGEIGGTMEEDL
                     AAYISKTGLKKKTAAFIAGRSAPEGKRLGHAGAIIEGRMGTAQSKIEAFAKAGVPVAE
                     VPWSIAELL"
     gene            complement(23261..24391)
                     /locus_tag="PKK36_01305"
     CDS             complement(23261..24391)
                     /locus_tag="PKK36_01305"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNR93227.1"
                     /translation="MKLYEYEAKDIFRKNGLRVPDGIAVTEPAQGLAFLEAHKPAVLK
                     TQVLTGGRGKAGGILFSDNAKDFSEKIERLKKLPIKGMLPRGILIEEKLDVRQEMYMS
                     CTISRSTAEILFMFSLTGGVEIESVAENNPEAIRKLSINVLDGMKPSQIEALVAKTGL
                     EKKIAAQVCEAALALYRIFTEYRCQIAEINPLVVTGDGTVVAGDAKMEIFDEAAKLHP
                     EYFKEDLTCTPLEKRARAMDLGYVELSGNVGVIGNGAGLNMATLDIISFYGAKPANFL
                     EVSGRTYGKAEDAVGIVVSNPNVKVVFGNFFGCISRCDVIAEGLAAAHTKGSIRMPMV
                     IAMRGTGAKEGLETLKKAGITEIYEDDIEAGRRVAELLKEAK"
     gene            24566..24991
                     /locus_tag="PKK36_01310"
     CDS             24566..24991
                     /locus_tag="PKK36_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR93228.1"
                     /translation="MQDLMIARKITCTLCDYNRLRSLLTTAQRYGLIRPALLRMLEDE
                     LGRAEIVASEEVPPYVVTMNTCVCLTDLATGASMQYKLVYPDDEDIRQGRLSILSELG
                     AAIIGFSVGDTIEWNFADGPRYIRIDMILYQPEAVHHFE"
     gene            25179..27014
                     /locus_tag="PKK36_01315"
     CDS             25179..27014
                     /locus_tag="PKK36_01315"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR93229.1"
                     /translation="MSKTLIMDVFGMIGGLSLFIFGMHVITGGLRAAAGPSLRTVIGR
                     TSSGPLRGALLGIILGFLAHSGAATTMIAGFVNAGLLGLQAAVAPIMGANIGTALSMQ
                     LLSFNIGQYCWVAIAAGFLLKSLLPGERCKQAGLAMIGFGLLFLGMITISGSLVPHRE
                     ALAPWLAHVRGDAWAGRLSGVGIAALLTAMMTSSGAMIGLCFALISAGIFQSFDQFFP
                     VIAGAHIGTCIVALLASLSMNIEARRAAAGHLFFNIFNVALALALYPFIRAAVVWSAQ
                     EPLRQAANLHLLMMATGALPLLPFTRACAWLLRRLVLPRSPMPEPSHLKPENLSRPEQ
                     AIRATILEVRRMARICIQGMTINGELMLDYRPATRRVLLANEAVIDDVKLAVGDYLDR
                     LTSYALSYRQTLFIQHLDRCMKDIERIGDYQTALADTTALRWHRREAQVPEDIFRAWF
                     DLFCAAQRVILLLERCLNPDDASFQEMASHLLEARDRFSALSARDKSAFALAARDRRI
                     TGTAAYYLHRYQADLDRMMGHVRSISVSLSQPEFWVRRSKLDRVEPSIRSGKQAARID
                     VKDYLARLYSDEVIEWKPDETLGEIESRQMPPPPPPEDTHVFATD"
     gene            27086..28345
                     /locus_tag="PKK36_01320"
     CDS             27086..28345
                     /locus_tag="PKK36_01320"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HNR93230.1"
                     /translation="MKKDNPSLPIEWIENTHAKKPGDCEPLARLLPPGITGVARQFHR
                     QLDGYRMSPLKSLSGLAAMLGIGGIWVKDESQRLSLNSFKVLGGSFAIYQFLRKRLGI
                     RDRELTVSELKSDKVRSQIGDITFATATDGNHGRGVAWAAMELGFKCVVYVHERTSKP
                     RIRAIEEYGAQVRVIPGTYDDAVRMITRDAAKNGWQIISDTSWVGYQDIPLWVMQGYT
                     TLMAEIQEQLAGQGITQPTHVFVQAGVGALAASVIGYYQGLFGKKRPVTVVVEPDRAA
                     CLYESAKIGDGKPHSVGGDLNTIMAGLACGDPSPIAWGILDRCADFFLSCPDFVAAKG
                     MRVYGVPLADDLFIVSGESGAVTLGALMFIAEHAGLSGLRKKMKLDSSSQVLLINTEG
                     NTDPEHFRRVVWEGGDPVPREFRHEPA"
     gene            28576..31653
                     /locus_tag="PKK36_01325"
     CDS             28576..31653
                     /locus_tag="PKK36_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR93231.1"
                     /translation="MNNCYQHVRGLTVSVLFLLVAIFTLAEAQAGSITGTVTGPDGLT
                     PLANIFVDSANVSTWDWGSSTYTDGSGHYELTGLAAGTYHVTFSDDGEVYAPEVYNNV
                     AGSDLWNDGTDIVVTETGTNTGINASLAIGSKITGKVTGSDGLTPLANIYVECVNKSD
                     SYWGPETYTDSSGNYELTGLAAGTYHVGFSDDDGTYAPEVYNNIPGDNVWNDGEDLVV
                     PAASTISNINASLVTASWITGIVTGPDEVTGLPNIYVQTYRWNGSWWSLAGASYPDID
                     GYYEIGGLGAGTYRVTFIDWNGDYVSEVYDNIPGDRYYDSGANIVVGEGSVNSGINAS
                     LAIASWITGTVAGSDGITPLEDIDVQAYHRSGPEWVYTGYTTTDTDGTYAIGGLMEGS
                     YRVNFHDWSGTYVPEVYSNMPGTDVWRYGTEISLPASVTVSNIDAFMDEYASLGGLVA
                     QADGLTPIAGVLVQMLGLGRLDGWVFSARTDVTGAYFCSSVYPGDYAVRTVPAASIGY
                     LGEWYDNKGLYVPGQETPPVGADVVTLTSGEDRTGINFALYPAGRVAGTVTGNGIPLA
                     GCPVKAKNGTYGLVRNAATGDAGTYELMGLLPGSYTLKAEADEYKDEWWSDALHEDSA
                     ALIAVASGDDLSYDFDLMPGQSPALVDVRCDPEVNAVVYVDYWPTTNTTPAIVDIGNT
                     VSRPADAGGWGIASHTVSIKKAGVPRSVPQIASAVEAETITMTFDVSSDDAGSVGVDT
                     TPAGSQVYIDYADVADGVTPLVVDNLAPGTHTILLKQQGYLQSRPVMVQVGADLTNMI
                     VVPLAPVSETNRIIAEVRSIPPHAEIYVDYLSGTNLTDAVIDWMDPASHAGAGWHSAS
                     HVILLRKDGYQSSSSYYPPEGTNDAGLLVVSLVGDTNVLVDLDHDGMPDQWEDSYDLT
                     NGWEVQISDGDYDGDGVSNYGEMIAGTHPGNLNSVFEMSEFDLSSPGNNEEVTFIFST
                     VPGRRYIIQGKANLGNNSEWIYLSGIIIATDYETSYTASMAGMRFFRLLVWAP"
     gene            31684..33735
                     /locus_tag="PKK36_01330"
     CDS             31684..33735
                     /locus_tag="PKK36_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR93232.1"
                     /translation="MQNESHEGISDSRFVVPKTSNWTAIICGVLLLWPSLSHISASSI
                     ALNYFDTPKRLLWSVMALVLALVARSEGCRLGARSAWLALGLLAWMIVRTLSRPAPAT
                     EIEVLFTWMLPLLMLILGAGIENSKGMRIFGALLVSAGIIQALLMVLQRFGLDPLFRQ
                     TTSAMDYAPGRMIGTIGYHNQAVDFLALSASGIFLLSKSSRLKLCFMLFMLFVAGLTA
                     NRGGILAFVFALAAVQSFAVWINPLWKLQKKALITASVILGICSVLGAMTLIPETGDR
                     FKDAVKDFRHSPAVQSRVLMNRIGCDMFTEKPWIGWGAGEYAHQYLDRLGNVLPEEKT
                     HEILGNVVFARETHNDYVQFAAEFGLLGFAILAALVCSIVISFIRAGNDQSDAVATAM
                     FILVYMAVASLFSFPWQTGMGGPLAGFLIGWLLPQKETSKNEVLRIWPKISKSALVAF
                     SLILVAWSAVDSFFNRSVPDILAEGHSERAARMLPRFAYRYRALVGAAYAVAGDYEAA
                     EKELTAAQKGYRDILLWNNLGNVYGKLGKWDEAVTVYERWARCGIDYDSALRNLSVAQ
                     EQAGTLEEAAETLARRISLWRTTPDEVKRLAVLQMQAGYLLKARETLFQYRGKWKSAD
                     PKTVAEIENLSGAISLNLEDYGEAERLFRSALQKYPGLESARRNLDQLRVITSAADSG
                     R"
     gene            complement(33707..34528)
                     /locus_tag="PKK36_01335"
     CDS             complement(33707..34528)
                     /locus_tag="PKK36_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR93233.1"
                     /translation="MKKCVISCVQMFIRPLDVTGNTERALYFLNKAKREHKSDLVVFQ
                     ESVTTGFTPNMSEKAFLKVIDRVPGKQIAPILDAAKKLKLHIVWPTYEKVGQKIYNSA
                     ILIGSNGKIIGKYCKTHPFDKESTERGGWTTPGSKMRVFQTPFAKIGMIICFDGDYPE
                     ASRLLAKKGAEIIVRPSALLRNYEIWSLTNRARAFDNHVFLAAANATGFDAAGRHYYG
                     HSMIVGPAGNIMALAAAQEDIISAELDPKMIRQTFPGSPARRVYDHLTDLNPRLK"
     gene            complement(34525..>35372)
                     /locus_tag="PKK36_01340"
     CDS             complement(34525..>35372)
                     /locus_tag="PKK36_01340"
                     /inference="COORDINATES: protein motif:HMM:NF028267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HNR93234.1"
                     /translation="AGHLKGKICQYWALNPHPTLIPAIESGFVERIYSFGGEPGMEEY
                     CKAHPDIFSIGPDGSMRSGRLNAQLLGLYGVDCFVGSTLQIDRCGNSSTAIDGMIAGF
                     GGAPNLGSTPPGRRHSSPTWEKSSRENKTFKKYTGYKSGRKIVVQMTPTVSEKKAIPV
                     FVEELDAAKLYRSGKFETPPVMLYGDSITHIVTECGIANLMKCASPKERMKAVASVAG
                     ETEVGKTSTPEDKAALRKKGILQTVEDLGIDPASATRDLLAAHSLEDLVKCSKGLYHI
                     PEGLK"
BASE COUNT         7568 a         9460 c        10702 g         7642 t
ORIGIN      
        1 attcccggac aagcctgtaa ctgcaaagcc cctggaagtc cgcatgggaa agggcaaggg
       61 cgcggtggag gaatgggtgg cggtgatcag gcccggctgc atgctttatg aactggatgg
      121 cgtgaccgag tccgtggcga gagaggccct gcgcctggcg gcggccaagc tcccgattcg
      181 cacacggttt gtgcagcgca acgtacacgc ataggatttg accagatatg aaggcaagtg
      241 aaatcagaga attaacaggc gaagaactgg cgcagcgcat cgatgacgcc cgcaaggagc
      301 ttttcaacct gcggctgcag cagaccggcg gccagctaga aaaaccctcg cgtatccgcg
      361 aggtgcggcg ggatatcgcg cgcatgagca ccgaggcgcg ccatcgccag aaagtttcga
      421 ggtagtcctt atgactgaag cagcaagaaa tcttcggaaa acacgcacgg gcattgtcac
      481 cagcgatgcc atggacaaga ccattgtcgt ggaggtgggc cgccgcatga gccatccgct
      541 ctacgggaaa gtggtaaacc ggaaaaagaa gtatcacgcg catgatgaga aaaacgaggc
      601 gcacaaggga gaccaggtcc ggattgccga aacccgcccc ttgagccgcc tgaagagatg
      661 gcgcctggtg gaaattatca agaaagcagc agcagagtga ggcaggtggg tcaatgattt
      721 cagtggaaac aaatcttgat gttgcggaca acaccggtgc gcgcagcgtc cggtgcatcc
      781 gcgtcctcgg acagcgcaaa acgacagccg gcgtgggtga cattatccgc gtggccgtca
      841 aggaggcgca gcccaagggt atggtcaaga agggtgaggt tcatctggcg ctgatcgtgc
      901 ggacaaagca tccgatccgc cgcccggacg gatcgctcct gcgctttgat cacaatgccg
      961 tggtgatcat agatgaccag aagaatccgc gcggtacacg catcttcggg ccggtggccc
     1021 gtgagctgcg cgagaagaat tttatgaaag tgatttccct ggcgcctgag gttctgtaat
     1081 gaaaaagaaa aaacaggtat tttccgcgaa accgcaaatc aaaaaaggcg acatggtgga
     1141 ggttcttgcc ggagatgacg ccgggaagac cggcaaggtt ctgcaggtga tcccgcagaa
     1201 gggcgcagcc attgtcgagg gcctgaatat catcaggaaa tgcgtgcgga agacccagga
     1261 taatccgcag ggcggaatcg ttgagcgcga ggcgcccctg gcgctttcga aactgcgtgc
     1321 ggcaaagagc gcaggcaaaa aggccgcggg agagaagaag taggccgcct gtctgcggcg
     1381 ggaatttgaa acgaaagggt tcgaagaatg acaccccggt tgaaggaaaa gtataaaaag
     1441 gaagttgagc cggcgctgcg caagcagctg ggatgcaaga atcccatgca ggttccgcgt
     1501 tttcagaaaa ttgtgatcaa tgtgggcacc aaggccacac tggacaagga ttctcttaag
     1561 gaaattgcgg agaacctggc gaagataacc ggccagaaag cggtggtcac aaagtcccgc
     1621 aagagcattg ccaatttcaa gctgcgtgac ggtatgccga tcggcgtcaa ggtgacgctg
     1681 cggggcgacc gcatgtttga attcatggat cggctggtga atgttgcgct tccgcgcatc
     1741 cgtgacttcc ggggcatttc tgcgaccggt ttcgacaaga gcgggaacta cacactgggc
     1801 ctgaaggagc acgatctctt cccggaaatt gatcctgacc gcgtcaaggt ggcgcacggc
     1861 atggatataa cgattgtcaa tacggccgcc aaccgcacgg aagctctcgc gctgctgaag
     1921 ctgatgggtt tcccttttgc gggtgaatga gcatgaagtt tggaggataa tcctttggca
     1981 aaaaaatcat tagtggtgaa atcaaaccgg gcgccgaaat tttcggcgcg gaaatacaac
     2041 cgctgccgca actgcggtcg gccgcgcgcg tatatcagga aattccagtt gtgcagaata
     2101 tgcttccggg aactcgcctc gcagggcaag ctccccggtg tcacaaaggc cagttggtaa
     2161 cggagcagtt atgagtttat cagatcccat tgcagacatg ttgacccgaa tccgcaacgc
     2221 cagcgccgcc ggcgcgaaat cggttgaaat ccctcattcg cgcatgaagg agggcctggc
     2281 ccggattctc aagcaggaag gttatatttc ggattacacg gtctcgggcg agggcgcccg
     2341 gaagggcttg cgcatccgtt tgaaatatgt gcgtgaagga gatccggtga tcaccgggct
     2401 ccggcgcgtc agccggccca gcctgcggcg ctacgtcgcc gccacggaaa ttccgcgtgt
     2461 tctgggcggc atgggcattg ccgtgatcag cacttcatcc ggcgtaatga ccggtcatga
     2521 agcccgaaag aagaatgtgg gcggcgagct tttgtgtaca atctggtaat tattcaacga
     2581 ggacacagct atgtctagag tcggaaagaa tcccgtggaa ttgcctgccg gcgtgacggc
     2641 ggaagtgggc gggaatacag tcaaaatcaa gggcccgaag ggggagctgt cgcttgatgt
     2701 ggctcccgga atcagtgtgg aggtcaagga cggccgggtg ctggtttccc gggcggcaga
     2761 tgacaagcag agccgcgcgc ttcacggcac agtccgcagc ctggtggcca acatggtcaa
     2821 gggcgtgacg cagggctacc gcaaggaact ggagatctac ggcgtgggat acaaggcccg
     2881 catggagggg cgcaaacttg tgctcaacct cggatattcg catcccatcg aatatatgat
     2941 tcctgaaggc gtgaagataa ctgtcccgaa gccggaagat gtgggcattg aaggtgtgga
     3001 caagcatctg gtggggcagg tggccgaccg gattcgcagc tttgccccgg cggagccgta
     3061 caagggcaag ggtgttcgtt acaagggtga aaagatccgg cggaaggccg gaaagacggt
     3121 ggcttaaaga tgaaagtcaa aacaaaaaaa gagtacattg gaaggagaca cgctcgtctt
     3181 cgcaagaaga tccgcgggac ggctgaacgc ccccgcatgt gcgtgaaagt taccagcagg
     3241 catatctatg tgcagatgat tgacgacgat gccggctgca cgctggcggc ggcctcttcc
     3301 atggtcaagg atttgaagtc cctcgcggca aagaagacgg aactggcgaa gcaggtgggc
     3361 aaggcggccg cggaagcggc cagagctgca ggcatcacgc aggttgtttt tgaccgcgga
     3421 gggtttacct atggcggccg gatcaaagca ctggccgatg cggcccgcga agcgggactg
     3481 cagttttaag gcttaaggag caggcattgt gaatgatata atttcagaga agacagaagg
     3541 gcacgaagtc gaagagcgcg tggtgcatat caaccgctgc tccaaggtgg tcaagggcgg
     3601 ccgtcgcttc agtttcagcg cgctggtggt ggcaggcgat catgggggcc gggtgggtta
     3661 tgccctgggc aaggccaacg aggtttccga cgccattcgc aagggcggcg aactggctcg
     3721 acgcaacatg ttccgagtga agatgctgaa tgagcgcact attcctcatg aagtgactgg
     3781 atcttatgcg ggcggcgtgg tcatgctgaa gcctgcatcc gccggtacgg gcgtgattgc
     3841 cggcggtgcc gtgcgcgccg tgctcgatct ggccggaatc cgggatgtgc tggccaaatc
     3901 catgggaagc aacaatcagc tcaatgtcac caaggccacc gtggacgccc tgcgccagct
     3961 gcgcacaagg gaagaagtgc agagcatccg gcgcggatag atttaaagag caggacacga
     4021 ttatgaaatt acatacactc aaaccctcaa ggggcgcaaa gcgctcgaag actcgactgg
     4081 gccgcggccg cgcttctgga aaaggaaaaa cctccggcca tgggcacaag ggccagatgg
     4141 cccgctccgg cgcgaatcac cgtgtggcat tcgaaggcgg tcagatgccg ctctaccggc
     4201 gcctgcccaa acgcggcttt aaccatgaaa aagccgttgt tctcgtgccg gtgaatgttt
     4261 cctcgctcaa ccggtttgat gcgggcacgg aagtcacctt tgagctgctg cagcagtccg
     4321 ggctggcccg cggcggcaag gccgacggcg tcaagattct tggaaacggc acgcttgaga
     4381 aaaaacttgt tgtgaaagca caggccttca gcgcgtccgc cgtcgaaaaa atcaaggccg
     4441 ccggcgggga gtgcactctg gtgtcataaa ctttgtctag gggaacccga tgctatcagc
     4501 cttttcaaac agtcttaaaa ttccggaact ccgggcgagg atattcttca ccctgggcct
     4561 gatttttatc tgccgtctgc tggcgcgtgt tccgaccccg ggtgtggatg ccgttgcgct
     4621 gagagatctg attgcctcgc tgcagacaca ggccggcgga agcatgctgg tgctgttcaa
     4681 cctgttcagc ggcggcgcgc ttcagtatgc ggcggttggc gcgctcggca tcatgccgta
     4741 catcagcgcc tccattattc tgcagctgat gaccgctgtg gttccggcgc ttgagcgtct
     4801 ggcccgcgag ggcgaggccg gccggcagac gctgtcgcag tatacgaggt atttgacggt
     4861 ggctctctgc attattcagg gctatgccat gtcggccacg ctctggcgtc ccgaggcgct
     4921 gggccttcct gcgggcacgc agattgtgac catggggagg atgggtttca ccctgctgac
     4981 cattctgaca atgaccaccg gaaccatgat gctgatgtgg ctgggcgaac agatgaccga
     5041 acggggcatc gggaacggca tctcgctgat cattacgatc aacatcatca gtgatctgcc
     5101 gtcggcggtt caggccattg tgagaatgtt cgtccgcgtg gacggtgtgg cccagttcac
     5161 gatcttccat ctggttggac tgctggcgat gatctttctg gtgattgcag gcaccattgc
     5221 cgttacgcag gcgcagcgga aaattccggt tcagtatgcc aagcgcgttg tgggtcgcaa
     5281 ggtgtacgga gggcagagca cctatatgcc tctgcgcgtg aactactccg gtgtcatgcc
     5341 cattattttt gcgcaggcca ttctgatgtt cccgtctaag ctgctgggca tgcttcccgg
     5401 agaatttttc cggcgattgg cggcggccgt gtactacgga actccggcct acaatgttat
     5461 ctatgccctg atgatcctgt tcttctccta tttctgggtc gccactcagt tcaatccggt
     5521 gcagattgcg gatgatctga agaaatacgg cggctatgtt ccgggaatcc gtccgggcaa
     5581 acctactgcg gaatttctgg accgcaccat gacccgcatt acactggccg gagccgtgtt
     5641 cctgacgatc attgctgttc tgcccaccat catggcgcag cagttcaata tcccctggat
     5701 tgtggcctcg ttcttcggtg gaacgagtct gctgatcatg gtgggtgtga tgctggacac
     5761 aatgcgccag gtggaatctc accttctgat gaggcactat gacggcttcc ttaagaaggg
     5821 caaactccgt tcgagacgct aaaggaggcc gagatggacg ccattattct tctgggagcg
     5881 ccgggtgccg gcaagggaac cattgcggat ctggtgaagg agaagagcgg atatcttcat
     5941 gtctccaccg gggacatgct ccgcgccgca gtcaaggcgc agacagctgt tggcctcaag
     6001 gccaaatcct tcatggaagc cggtgaactt gtccccgatg aagtcatcat cggcattgtc
     6061 agggaacgca tggctcaggg cggacaggat gagaagtaca tgttcgacgg atttccccgc
     6121 accctggagc aggcgcgcct gctggatgcc gtgatggagg aggcagggag ccgggtcagc
     6181 cgggttttcc ttctggaggc atcaaaagaa ctgctcgtat cccgtatttc cggtcgtcga
     6241 atctgccgca actgcggagc ggtgtatcac gtgcagaaca ttccgcccaa agtggagggc
     6301 gtatgtgatt cctgcggcgg cgagctttat cagcgcaagg atgacagcga ggaaacggtg
     6361 ctcaaccgtc tggaagttta caagaaacag accgcggcgc tcattgacta ctacgatgcc
     6421 aagagcatcc tcctgcgatt ggatgcttcc cagcccaagg aagtgaccca ggccagaatt
     6481 ctcgaagttt tgtcgcgttc ctgatttgtg tttcatgatc aggattaaga cggagactga
     6541 actggagaag atgcgcgtca gcaaccgcat gacggcagag gtgctggcgg cggtggctgg
     6601 gcgggtggcc ccgggcatta ccacgggtga actggaccgc tttgcggggg agatgatccg
     6661 ggacatgggc ggcacccccg cgtttcttgg gtatggaggt tatcccgcca gcatttgtgt
     6721 ctccatcaat gaacaggtgg ttcacggcat tcctggccgc cgcgtcatca gttcaggaga
     6781 tgtggtcagc attgatgtgg gcgtgaaata cgacgggttc attggagact gtgcaacgac
     6841 cgtgctggtg gatgtggatg accgggagat catccgcctg gcggctgtaa cgcgggcggc
     6901 gctggcggcc ggcatagaca aggcggttgt tggcggccgg ctttcggata tttctcatgc
     6961 cgttgaaaag accgtcacgg cggcgggctt ctccgtcgtg agggattttg tcggccacgg
     7021 aatcggccgg gacatgcacg aggagccgca ggtgccgaat ttcggcaggc cgggacgagg
     7081 accgaagctg gcgcatggga tgacgatagc catcgaaccc atggtgaatc agggcgggtg
     7141 cgaagttgaa gtgatgccgg acggatggac ggtgctcacc cgtgaccgga ggccttcggt
     7201 ccatttcgag cacaccatcg cggtgctgaa aaccgggccg gaaatattga cgtgcgtaaa
     7261 aaagaaaata tagtactgga cgcgcggctg cacaatgtga taggaaatgc cgcctttaaa
     7321 gccactttgg ccaatggaca tgaatttgtc gcctttgcgc gcgacggaga actcctccgg
     7381 aagctgcgcg ccggggattc agtgaaagtt gaattgtcgc cctgcgatat gtcaaagggc
     7441 tgtatagtga acaggacaga aaatgaaagt tcgaagctca gttaaacgga tttgcgagca
     7501 gtgcaagatt gtgcgccgga aaggcgtggt gcgcgttgtt tgcacgaatc ttcgtcacaa
     7561 acagcgccaa ggttaaagga gagaagctat gccgcgtcta cttggtatag acatacccgg
     7621 aaaaaagaga gttgatgttt ccctgcgtta catttacggc atcgggccca agcgtgccct
     7681 ggaagtggcc gccaaggccg ggattgatcc cgcaaagaaa gcggatgacc tgaccagcga
     7741 tgagatttca aaaattgccg gcattctgca gagcgaatac cggatcgagg gtgatttgcg
     7801 ccgcgaggtg tcccagaaca tccggcggct catgagtgtg ggcgcctacc ggggcattcg
     7861 tcatcgcaga agtctgccgg tgcgcggtca gcgcaccagc accaatgccc gcacgcggaa
     7921 gggcccgaaa aagaccgtgg gcgccgtgcg tggaaaggaa gcgcgttcgg cagtcaaggc
     7981 cggttgatgt tgttcagata aagaggttgt aattcatggc agaagagatt cagaaggaac
     8041 aggcggaaac agcggaacag gcagctgctg aaggcagcgt gcaggcggaa ggcgccgagg
     8101 gcgagcagcc caaggtggtg cgccgtaaag tcaaggggat gaagaatgtc ccgctgggca
     8161 ttgcccatat ccgcgccacg ttcaacaata ccattgtgag catcagcgac atgcagggcc
     8221 gtgtcatcag ctggagctcc tccggccgct gcggcttcaa gggttcgcgc aagagcaccg
     8281 cttttgccgc gaccacggtt gcacaggaag ccgcccgtgt ggcggtgggc ttcggaatgc
     8341 atgaggtgga ggtgcgtgtt caggggccgg gtgccggccg cgaatcggcc atccgcgctc
     8401 ttcaggccgc cggcctgact gtgacctcga tcaaggatgt aacccccata ccccataacg
     8461 gatgccgtcc gcccaagcgg cgccgcgtat aaagaattta gaatcaggag acagaaagcc
     8521 atgggaagat atacaggacc agcgtgcaag cagtgccgcc gtgaacagat gaagctgttt
     8581 ctcaagggag agcgctgttt catggccaaa tgccccattg agaccggccg tccgcagccc
     8641 ggaggcgccg gccgccgtcg caagaagact tccgattacg ggaatcagct tcgcgaaaag
     8701 cagcggctgc gccgcatgtt cggcctgcgc gagggccagt tccacaactt ctttgaacgg
     8761 gctgccaaaa agcgcggcat caccggcgaa acactgcttc agatgctcga actccgcgtg
     8821 gacaatctgg tgtaccgtct cgggtttgcc ccttcgcgcc gtgcggcccg tcagtttgtg
     8881 cggcacggac atgtccgcgt caacggggag cgggccaata tcccgtccat gatccttaag
     8941 ccgggcgatt ccatcacggt caaggacgcc aaggacagcc gggaatacgc ctccaagcat
     9001 atggaccagg ccgaggctcg cggaatcgtt ccgtggatgt ctctcgacaa gaacaatttc
     9061 aagggcgaag ttctgcatgt gccgacacgt gatgaaatgg cgcctttggt ggatgagcag
     9121 ctcgtggtcg agctctactc caaataaaca agcagtataa caccatacga gccgggtaac
     9181 gtccggttct gggaggataa agtcatgcct attagactgg gtcgctttga aatgccaaag
     9241 cgcattgagc cggatgattc aacggctacc gatacctaca agaaattcgt cgccgagcct
     9301 ttcgaggccg gctatgggag aaccatcggc aattcactcc gccgtgtgct gctctcctcg
     9361 ctggaggggg cggccatatc gtctgtcaag attgatggag ccatgcatga gttctgcact
     9421 ctgaaaggcg ttgtggagga tgtaacggac atcatactga acctcaagca ggttctgctg
     9481 cgcatgtatt cgcgcgaaat ccgcaaggtc cggattgatg tcaagggccc gggcgaggtc
     9541 acagcgggcg atatcaagac cgacggccat gtcgaaatcc tgaatcctga catgcatatc
     9601 tgcacactcg accgcgatgg tcacttcaag gctgagtttg aaatccgcat cggccgcgga
     9661 tactgcccgg ccgagtggaa caagaaagag gaccaggaga tcggtgtgat tccgattgat
     9721 tccctgttct cgcctgtccg ccgcgtgaag tactatgtgg agaacacccg cgtgggccag
     9781 cgtaccgatt acgacaagct gattcttgag atatggacgg acagccgcat tactccggat
     9841 gatgccctga cacagtcggc ggccattctg ctgcaccatc ttgatgtctt tgtcagctac
     9901 gacaaggatc tgattgagtt tgaggaaagc gaaaagcaga ttgatgccga gaaggaagat
     9961 ctgcgcaaga agctgaacat cagcgtcaat gagatcgagc tgagcgtgcg cgcggccaac
    10021 tgcctgaaca atgcaaacat tacaaccgtt ggcgaactgg cgcagaagac tgaagccgaa
    10081 atgctgaagt accgcaactt cggcaagaag tcgctgaatg aaattaaaaa caaactcacg
    10141 gagctgggcc tttcactggg catgaccttt gatccggaac tgctcaaacc ggtggctcct
    10201 tccgccgagg acaactaaca cgaggcagga ttaaccatgc gacaccagaa gagaacaatc
    10261 aagctgggga gaacatcttc acaccgcgat gcgctgctgg ccagtttggt ctgcgcgctg
    10321 atccgccgga agcgcatcag gaccacgctt cagaaggcca aggcggcaag aatcacggca
    10381 gagaagatgg tcactctcgg aaaaaagggc acactggctg cgcgccggca ggccatatca
    10441 aaactgcgtg atacggaatg cgccgctttg ctctttgcag aaattgcgcc gtcattcaag
    10501 gatcgccagg gcggctatac gcgaatcatc aagctgggac agcgtcccgg cgacaattcg
    10561 caggtggcta tccttgagtg ggtgatggaa cattcggttc aggccacgaa gaagaaaaag
    10621 agtgcggcga aaaaagaagc agaagcggca gagaaggctc ctgagaagaa ggctccggca
    10681 aagaaaaaag cggccagcaa ggccaaaaag gaagagactt ctgaaaaggc tccggccagg
    10741 aagaaaactt cccggaagaa aaaagaagaa tagtttccgg aagcggtttc aaacagcccg
    10801 gcgcagtttc tgcggccggg ctgcttcttt actgctgcgg gggaactgaa ggcgattgtg
    10861 cttcggatgc ggcactggcc gcggcggccg ccatggcctt gctgctctgg gaccggaact
    10921 gttccgccag cacggcgggt tccggcagtt ccggccgctt gctgtcatat ttgaacgtgc
    10981 tgcaccgcca gcgtttcccg ttgggatgaa ccatgaacac agcaggcgtt ttgttgaccc
    11041 tgaacatctc ggccatggct tcggtttccc ggttgctggg cagtgtcgcc cgcaccttga
    11101 taacctcctt gaaaaatttc tgcacgggtg aggtctggaa tcctctggtt tccagccagt
    11161 tgcaaagacc tttttcatcg gacacatcgg gccggacgaa gaacacaaaa atatcggctc
    11221 cagtctgttt ctggatttcc agcgcttcct gatatccttc cgcgtttttc agccatttgc
    11281 tcggcagatt cacgggggct ccatccgatg aggaggggcc gaccggctgc gtggcgaccc
    11341 ccgcgggctc cggcaggccg gccttgttgg ttcccagttt ctcgcggata ttaatgcgcg
    11401 cggatgccgg gggacgagcc tgaacggcag cggccagagc catgacggaa aaaacaactg
    11461 ttatcgttcg catattcttc tcctgatttt ccataattct gccattgatc cgggcggatg
    11521 tgaaggaata tgaactgctc agccggattt attccgcatt ggattcctgc ctgaaatgct
    11581 atactccgcg catgggaaaa aatgtgggac aaggcatttg cactctgatg atttttgtgg
    11641 acggtctggg gctggggcat tctgatcctg aagtcaaccc tgtgttttcg ggtgcctgcc
    11701 cggtattatg cgaactgctc agggaggccg ctgccataga tgccgtgatg ggggtcgaag
    11761 gccttccgca aagcgcgact ggacaaacgt ctctgctgac gggtgtgaat gcccaggcac
    11821 agatgggccg gcacatcgag ggatttccca acagggagct gcgcgacatt atacggcggg
    11881 agaacatttt cgggaaaatg atggagcggg ggtacagcag cactttcgcg aatgcctatt
    11941 accttcaggc atacacctcg gcggaattga ggcgccggcg atccgtgacg accgtggcca
    12001 ccgaagccgc ctttggaaaa ttcaggagcc tggaagacat gctggccggc cgggccgttt
    12061 atcaggattt gacacgcgag cagcttcgcc gccgcgggta tgaagggccg ctgattgaac
    12121 cggaggaggc tgggtatcat ctggtggaga ttgcgagggc gcatgatttc acgctgtttg
    12181 aatatttcca gactgacctc gccgcgcatc ggggcgaccg tcaggcgctg ctgtctgtcc
    12241 tggaaaaact ggaccgtttt ctcgggattc tgctgcatat gtttcgaggc tccaacgagc
    12301 tgttcctgct gaccagcgat catgggaatc tcgaggattt cggcacgcgc ctgcacaccg
    12361 gcaatccggt gccctttgcg gcaaggggca gggaggcgtc ccggctgaag gcgaaaattc
    12421 attccctgaa ggatgtggtt ccggcgctgc tggaattata tcctccgggc cattctattg
    12481 cttgaaatac cggggcccga tggggcattc tggtttcatg agtaaagcag cagacacgcg
    12541 gccggactgg gatacctact tcatggaaat cgcgcatgtg gccgccacgc gcagcaactg
    12601 ctcccgtcgc catgtggggg ccatcgtggt gcgtgacaag cgcattatta cgactggata
    12661 caacggcacc ccgcgcggtg tgcgcaactg ttttgacggc ggctgccccc gctgtgcgag
    12721 cgattcgccc tccgggaaca gcctgggaga ctgcatctgc gcgcatgcgg aggagaatgc
    12781 gattgtgcag gcggcatatc acggcatatc cgtgaaggac gccgcgatgt atgtgaccat
    12841 cagcccctgt ctgatgtgcg caaagatgat catcaatgca ggcattcggg aagtggtgta
    12901 tgagggtgaa taccgattca cggaatccac gcggtctctt cttcaggagg gcggggtgat
    12961 ctgccgtcag ttcacccgtg ccgcggggtt taactccagt actggtttgt gatcggcatc
    13021 cgcctttcgc ggccgaaagc gcatggcgtg atcttgatgc ccggcgcccc ctgccgccgc
    13081 ttgtattcgt tccggtccac agtgcgcacc gtcatgcgca caatatcccc gtcgaaaccc
    13141 ctggccatga tttcctccag gccgcagtgc tgctcgatat agagctcgag aatcggatca
    13201 agtatttcgt aggggggcag ggtgtcgctg tccttctggt ccggcctgag ttcggcgctc
    13261 ggcgggcgct cgatggtgga cggggggatg accggaacgg tgtcttgtga gttccgccag
    13321 cgtgcgagct caaataccag tgttttgggc acatccttga tcacggcaaa gccgcccacc
    13381 atgtctccgt aaagcgtgca gtatcccatg gccagttcgc ttttattgcc ggtggtcagc
    13441 accagccagc cgaatttgtt ggagagcgcc atgataatgt tggcgcggat gcgggcctga
    13501 agattttcct cggtgatatc aggcgccttc cctggccact gcccggtgag ttcattcagg
    13561 taggccttga aaagagattc gattgccacg gtccggaaat caattttcag attgcgggcc
    13621 agctgcacgg cgtcggcccg ggtggcttca gaggaaaacc gcgacggcat ggtgatgccg
    13681 tgcacccgct ccgcgcccag tgcatccgcg gcgatggcgg ccacaagggc ggaatcaatg
    13741 cctccgctga gcgcaacaac acaattctga aaagcgttct tgttcacata atcccgaagc
    13801 ccggtcttca gggcggcata aacctcctcc gcagggccca gcgcgggcgc gaccgcggaa
    13861 tcagcgggag aggatgtgat ctccgccgcc ccagtcaccg ggatatctat gatgtcagca
    13921 tccgcctgct tggaatgcgc gcctttgcgc ggcgccggaa gattgaaata gagagtttcc
    13981 tccgcgaact gacgtccgcg catgcatgtg ggccccttcg ggctgacgac catgctggcc
    14041 ccgtcaaaga ccagttcatc ctgtcctcca accagattgc agtagagcag gagggtgtcc
    14101 agcttgaccg cggtcttctg aagggccgcc tcgcgccggc tgagtttgcc ccgctcgtac
    14161 ggggaggcgg agatgttgac cagaccgtca agctgaaacg gcgcaagaag ccggcaggcc
    14221 ggttcgtccg gaagccagct gtcttcacat atatgaaccc cgattcgcgc cccgccgaag
    14281 ttcaggatca gggggcgttc acccggaaca aaaattcttt tttcatcaaa tacaccataa
    14341 ttgggaaggg cgattttttt ataaacgccc cgaacttctc cgccggcgca gaccaccgcg
    14401 ccgttgaaga ctttctgttg ctctgtccat ggcgcgccga atatggtgac ggagcctgcg
    14461 gggagccggg cctgcaggtc gtgcagcgcc gcagtgcagt cgcggataaa atgcggtttc
    14521 aggacaaggt cttccggggg gtatccgcag agggcgagtt ccgggaaaag gatcaggcgt
    14581 gcgccccggg cggcggcttc acccgccgcg gccgccatct tgtccgcatt ggctctgatg
    14641 gcgccgaccg ctacattgat ctggcagagc gcgacagtga tctttttcat tcccgacatg
    14701 atacaacgaa cgccgccggc gcacaactgc cttgctgaga ttcaggcgat tgacttggag
    14761 ccccttcgcg cctattgtat gctgctgagg ataacgggaa atgagtgata taacggatga
    14821 caatctggag cctgtccccg ccgacaggat gtcgagcacc gatgaccagc gcatcgagaa
    14881 cattcttccc ctgcctccgc cggcgcacct gatccgattt ttcccgatca aggacactcc
    14941 tgctgaaaaa ttaatcggga ccacccggcg cagaatcagg tctattctgc gcggccagga
    15001 cgaccggctg ctggtggtga tcgggccgtg ctccatacat gatccggcct cggctcttga
    15061 atacgcggaa cggctgaaaa ccgagcgaga gaagcgccgc ggtgagctgg aaatcctgat
    15121 gagggtatat tttgagaagc ctcgcacaag catcggctgg aaggggctca tcaatgatcc
    15181 gtatatcgac gggagcttca agatcaacga agggctgcgg attgcgcgtg atctgctggt
    15241 ccggatcaat cagctgggga tcccggccgc ctgcgaattt ctggatgtga tttccccgca
    15301 gtacatcggc gacctcgttt cctggggcgc gattggcgcc cgcaccacgg agtcgcaggt
    15361 tcatcgtgaa ctggcctccg gcctttccgc cgtggtggga ttcaagaacg gcacagatgg
    15421 aaatattaaa atcgccgtgg atgccatcct tgccgcccgc gaacggcatc acttcctgtc
    15481 cgtgcataaa tccggtcagg tggccatcgt ggacacctgc gggaacagcg actgccatgt
    15541 aatactgcgc gggggcaggg tccccaactt tgatcccaaa agcgttgaag cggcgtgctc
    15601 cgaactgaga gcggcccggc tcccggagcg catcatgatt gatctttccc atggcaacag
    15661 cgcaaaggat catcgcgagc agctcaaggt gggagccaat gtggctgcgc agatggccgg
    15721 cggcgagatg cgcatcatgg gggtgattgc cgaatcaaac ctgatcgagg gccggcaggc
    15781 catatccgcg aagaagcgcc tggtgtacgg acagagcatc acggatccgt gtcttggctg
    15841 ggaggattcc gttcagcttc tcgggcagct tgcagatgct gtgcgcgccc ggcgcaaggc
    15901 ccgcaaatag acaccggccg cactcgcgcc gcgggcgcgg gggcttacag gctgttccca
    15961 aaatcctttt tgaactttgc catcagcttc tgcggccagt cgctggtggg ggtcaggcgg
    16021 cccgtgaaaa agcggaggac ggccggttcg tattcaaact gaaggccgcc aatgagtttc
    16081 cggttcttgt ggagccactg aagccggtcc gcgacatact tgacctgcga gagggtgaac
    16141 acgcgccggg gcatggccag ccgcagcagc tccatgtcgg ccagaatatc cttcccgctg
    16201 acatcacgga cactcgaaat ggtgccgcgc tccatgccgc gcacaccgga tatgatataa
    16261 aaagccgccg tcagggcccc ggccggatat tcggtctggg gcacgtgcgg gagaaaacct
    16321 ttggcatcaa tatgacagcc cagccctccg gccggagtca ccaccggaac gccggcggca
    16381 tcgagatgct cgaccagata tttgatgaac tgcggggact ggctgatgca ggtttcgtcc
    16441 tgggtttcgc gcaggcccac ggccatggcc tcaatttcgc gcacggacat tcctccatac
    16501 gtcaggaagc cctcgaaaag gataacaaga tccttgagct tgtcgtagag atggacatca
    16561 ttcgtgcaaa tgccgcctcc gcgcgttgaa ctgacctttc ggctcgaaaa gtagaccaga
    16621 tcggaaagat cgcacatggt tcgcaggatt tcggcgattg atttcttttt gaaggcggct
    16681 tcgcgctgct tgatgaaata ggcgttttcc ccgatgaggc tggcgtcgag caccagcata
    16741 atcttgtgct gatcgcacac cttgcgaacc tgccgcatgt tctcgatcga aaagggctgc
    16801 cctccgatca gattcgttga agcctccatc cggacgaatg gaatccggtc aacgccgtgc
    16861 tttttaataa gcttcttgag cttttcgatg tcaatattgc ccttgaaggg gtggctgctt
    16921 ttgattttaa gcgcctcgct tgtgaaaatt tcctcgatca ccccgcccgg gagttcgatg
    16981 tgcgccttgg tggtggtgaa gtggtagttc atcgggatga catcaccctg ccggatgaag
    17041 gcatggaata tgatgtgctc cgcggcgcgg ccctgatgcg ccggcaggaa atatttcttc
    17101 ccgaagacct ctttcacggc cttctgcagc cgttcaaagc tctgggaccc ggcataggcg
    17161 tcgtccgcca cgagcatgga ggccacctgc tggtcactca tggcgttggt tccgctgtcg
    17221 gtgagcatgt ctagaaacac atcctgcgtg ctcagcagga aggaattaaa gccggcgtcc
    17281 tgtatggccc tgaggcgctc attcaccggc ttgaggttca gtttctgaac aatgcgcacc
    17341 ttgtgcagtt ccacggggat ctgttccccg acgatgaatt tgattaccgg ggagtcgtgg
    17401 gctgctggac tggtattttt cttcatacag tggttatcct ctctgtttcg gatgggggat
    17461 agtgccgaca gaacgggatc aaatcaagcg ttgattttgc cgaccgcggg ggaggcggcc
    17521 ggctggccgt gaaaaatttc tgtgtcgatg tgcagacggc tcaccgtgtg cagatgcagg
    17581 gaaaggtggt ttttcagatc ttcaatgatt tgcgcgtggg cggtctcggg tgtttttgtg
    17641 gccatgacga tgtcggccgt gagaacgggc ccgccctctt catctgtcag ccgaaggtca
    17701 tggaacgatg cgatctcgcc gtgcgccgag atgtagcggc tgaggacgga ttcggcctcg
    17761 ggatagaggc ggtgagagcg gtccacggga tccacatgga caatcgcctt ggcatgacag
    17821 ttgtcctcga tcttctgttc aacctgggcg gcgatccggt gcgcctccag cgcggtcccg
    17881 cacgcatcca cctcaatatc aagcgatatg agccggtagt cgccgtactt gtgcacgatg
    17941 atgtcatggg catcgcgcac gccgtccacc tgaaaagcaa gcttttcgag ctggctgatc
    18001 tcttcggcgg agggggcctc tcccagcagc aggctgaccc gctcgcggat gagcgaaagg
    18061 ccgccatgca tgatgaggcc cgcaatgatc aggcccatcc agccgtccac ggcggcccag
    18121 ccgaaacgtg aactgaagag agccacacag accagcccgg ccatgaggga atcgaagcgg
    18181 aaatgccagt agccggcttc gatcagcgac gatccgctgg ccctggagag actgcgggaa
    18241 aagtgggcca tccactcctt cagggggatg ctggcggcca caatggcgat aagccaccac
    18301 ggggcatcat aggggtgggg ccggaaaatc cggaccaccg cttccttgcc aagcgaaagg
    18361 cccacgccga cgagaagaat ggaaacaatc agcgaggcaa tccactccat tcgcccgtgc
    18421 ccgaaggggt gctcccggtc ccgcggcttg ccggcccagt gcgcgccgac tatgatcatg
    18481 gtggaggccg cgacatcgga aaggctgtca aacgagtctg caatgagcgc gatgctcccg
    18541 aatacaaagc cgaaggtgat tttaaccaga aaaatgaaaa gattggcgat gattcccacg
    18601 gcgccctgca gcatgccgag gcggctccgg gagtcacccg tccggaggtc ggcgccggat
    18661 ttcagaaccc gccgggccag ccagccggta aagccccgta tctgctcctg atgttcatta
    18721 ttcatcatgg cgggaagtct atccgtattc tgcccctgtg caatcctgtt ttccgggttg
    18781 cttgttgaac atgaatatac tatcaactgt caatcagcac agataaaggg caagaggatt
    18841 tcatggcgtc cagaaaagaa gagcagtttg aaggttcgtt tttcgcaaaa ccggcggtgg
    18901 gttcactttt cacggtcttg tcggcgctgg cctttctggt gctgtgcatg gtgctgccgc
    18961 tggtggggca ggccggggtc gcgacggagt atgcccgtca gaatttctac accttcatgg
    19021 ccgtgctggc cgtctgtttc gtgctggccg tgctggcggt ggtctccaaa ctggagcgcc
    19081 gcaggctgga cgaaagtcct ctcccgtatt cttcgctcgg cctgtgcgcc gtttgcatcc
    19141 tcctgttcat tgccctcgtc agcggcatgc tgggggtttg accccgtgtg atttggattg
    19201 ccatcggagt gattcctgct acctttctgt tttcggtttg gtttgaagaa ggagtttaag
    19261 caatgaacag catgaaaatc tgggttttga cagcgctgat ggccgttccg gcggtttttg
    19321 ccccggcagt ctccgcggcg gaatatgatc ttttcgcgca ggctccctgg tcggtcagct
    19381 atgaaatcgg tttctacaat tttgagggcg atgaagaggt tgaagatgcc ttcttccatg
    19441 cgttgagact gggttatgac ttcaatcccc gctggacctt tgagctggat ttcaaccttc
    19501 ttcccaaact ggagcccacg gattttcagg acccgcggcg ttcggacatt gatgacgaca
    19561 cgtgggcgat tcgccttggc cttgatgtcc tgttccacct tcgcaacatc gagaatctgc
    19621 gctgggatcc ctatctttcc ctcggtgcag gctggtttca ttacgggaca agtgatgccc
    19681 aggagcagga cgagcctatt atcatcggcg ggctgggcat gttctatcac ttcaacgacg
    19741 agtgggccgt ccgcatggat gtccgtccgg tggtacaggg cacggatacc gaggggaata
    19801 ttcttttcag cgccggcgtg aactggcgct ggggcgccca tgtgcccagg gtgtatgaac
    19861 tggaaggcac cgatatagac agcgacaacg acggcctgct ggatccccgc gaggctgaaa
    19921 tcgggaccga tccctacaat ccggataccg acggcgacgg cctgaccgac ggtcaggagg
    19981 tgctgagcta cagtaccgat ccgctgaatc ccgacagcga ctgggacggc ctgaaagacg
    20041 gcgcggaagt tctgacctac cgcacggatc ccaacgaccg cgacaccgac aacggcggcg
    20101 tggccgacgg gcatgaggtg attgaggatc ataccaaccc gctggatccg gcggacgact
    20161 tgcagctctt cacgctgaat atcgagtttg actacaacaa ggcggatgtg cgtccgcagt
    20221 attttgaaca gcttgatgtc gtggcgaaag ttctgcagcg cgatccgggc gccaccgcaa
    20281 aggtggaagg ccatgccgac aagcgcccga cctccaagcg cgactacaac atcagactgt
    20341 cagagcgccg cgcaaaagcg gtcatgggat atctgatttc ggccggcggc atcgaaccct
    20401 cgcggctttc gtcgaagggg ttcggctttg acgttccggt ggctccgaat gacaccgaag
    20461 agaacatgca gaaaaaccgc cgcacggaaa tctatatccg gcccagcaag agcgccgaaa
    20521 cgggcgggga catggatatg agccagccgc aggaaatgcc cgaagagatg cccgtcaaat
    20581 aacggagccg ttcggctgtg ccgatctacg aatatacggc ggttgactct tcaaaagcct
    20641 gtgaatactg ccgcaaggtg tttgagcacc ttgcggcagt gaagcaggaa cccctcaaga
    20701 cctgtccgca gtgcggttcg cccgtcaagc gcgtcatttc cgcgcccaac gtcggcggat
    20761 caaaaagcgg cctggatgac cgcgcgaaat cggccggttt ccacaaactg aaaaaaatcg
    20821 gcaagggcga atacgaaaag cagtattagg ccggaaaacg ccaaaaaacg gcttaaaaac
    20881 gtaaaaaatc agcaaaaatc gccaaaaaag ctcaaaaatg gcccaaaaat ggccgaaaaa
    20941 tcgcgaattg cgccgaaatt ttgaaaaaaa gtccgaaaat gtgccgtttt gattaaaata
    21001 ggactaaaat agtcctattt aaaatatcat gcgtccgccg cgcgggtgca ggcaaaaatg
    21061 cattcagaga cgggctttcg tgctatttcg cggtgtcgtt ttccgcagcg gctgtttccg
    21121 gcggagaaag ttcctcatcc gtcacgccgt gggctttgat ccggcccgca atgtgagccg
    21181 gaacgacaca aacggccatc agcggatagc tgaggaccgg actggtgaaa tagctgatgt
    21241 cggacgcaat gtaggtggcg taggccggat acaggatttt gtagcccccg cgctgaagtt
    21301 catgatcggt ttcctgcgca tagctgagcg cgtcgttcga ggcctgagcc tcatgccaga
    21361 gatttacaaa aggaatcaca tagatgaatg cgtaggttcc cttcagcttc ttctccgcga
    21421 agtcctttga gtgcccgccc tcgtgcacgg ttacggcggg gatgtctgaa tatatgttca
    21481 gcgtgttgga gtaggggttg tagttgtctc cgccgaagaa gcgctgcggc agaatcgtat
    21541 actgcagcca ccccagcagc ccgatggtgt accgccagcc gaagcccacg gatttgttgc
    21601 cgagcgtccg gctgagttcg gctccgggat gatactggtt gatccgcacc ttgacgctgt
    21661 cgagatggtt cgcggccaga taggagcgca gagtatcctc cgtctcctgc gatacctgat
    21721 ggctgtccac tttccgattc cagagaatca gcttgcccag cagacttccc ggccagatcc
    21781 agtcgcacgc gtcgagaaac ctgttcggct tccctcgctc aatctgctgc tcgtgctccg
    21841 acaggggcgg ggccggatga taatcataaa ccgcgccgaa atgatacggg gtcgtggcgc
    21901 agccgctcag cagcaggacc gcaagaatca gagaaccggc tttggaagtt ccctgcatgc
    21961 cgtccagccc tagagatgaa aatccccggc ggcttccggc tgataaagaa ctttctcgat
    22021 ctttatccgt cttttgcccg caggcaccgg ccactcgacc gtttctcctt cggaataacc
    22081 cagcagggct gtgccgatgg gggaaagcac ggaaatccgc ccggcctcga aatcagcatc
    22141 tgcggggaaa acgagtgtgt attcctcctc ttcctttgtt tccagatcgc gaatcttgaa
    22201 acgcgagttc attgtgatca ccgtcgccgc cacgtctttg gactccacca aatggccgca
    22261 atccagttcc gctgccagtg ccttcaggtc gctgcgggtg cggtcaaatt cgttggcaac
    22321 cgccagcagt tccttgaggc gggcaaaatc aaatttcgtg atggatattt ttctgttctt
    22381 catggtttct cctgttgcgc tttttacagc agttcagcaa tgctccaggg cacttcagcc
    22441 actggtacac cggcttttgc aaaggcttcg atttttgact gcgcggtgcc catgcggccc
    22501 tcgatgatgg ctccggcatg cccgagccgc ttgccctcag gcgcgctgcg gcccgcgatg
    22561 aatgccgcag tctttttctt gagccctgtt tttgagatgt atgcggccag atcctcttcc
    22621 atcgtgccgc cgatttcgcc gacgaccacg attttctccg tctcgccatc ctgctcgaaa
    22681 agcttgtaga tatcaaggaa tgtggtgccc accatcatgt cgccgccgat tccgaccacg
    22741 gaggattgcc cgtagccatt tttctggagg taatagcaga tttccacggt gagcgttccg
    22801 cttctcgata cgactccgac agggcctttc ttcacaaagt cggacgccag caccccgatc
    22861 ttggcctcgc cgggcgtcag caggcccggc gtgttgggac cgagcacgcg cacccccctg
    22921 cgccgagcga gggatatgaa gcgcatggcg tcatgcggac agatgccttc cgtcaccgcc
    22981 acgatcagcg gaacccctgc ctccacggac tcttcaacgg ctgcagcggc caccctcgcg
    23041 ggaacaaaca ccccgcacgc gtcaatgtcg tgtttcgcca tcgcttcgcg gaccgtgttg
    23101 taaacgggca ctcccagatg ttcgatcccg cccttgcccg gcgtgactcc gcagacaatt
    23161 cttgtgccga ggtccatcat gagcttcgca tgataggtgc cttccctgcc tgtgaggccg
    23221 gagatcatga cgctgctttt gccgttgatg agaatggaca ttatttcgcc tccttcagca
    23281 gttcagcaac gcgccgcccg gcctcgatgt cgtcctcgta gatttccgtg atccccgcct
    23341 ttttcagggt ttcaaggccc tcctttgccc cggtgccgcg catggcgatg accatcggca
    23401 tcctgatgct ccctttggtg tgcgcggcgg caaggccctc ggcgatgaca tcgcaccgcg
    23461 agatgcagcc gaagaaatta ccgaagacaa ccttcacatt cggatttgaa accacgatgc
    23521 cgacggcatc ctcggccttg ccgtacgtgc gtcccgaaac ctcgagaaaa ttggcgggct
    23581 tcgcgccgta aaagctgatg atgtccagcg tggccatgtt cagtcccgcg ccgttgccaa
    23641 tgactccgac gttgccgctg agttcaacat agcccaaatc catcgcccgc gcccgctttt
    23701 caagcggggt gcaggtcagg tcttccttga aatattccgg gtgcagtttg gcggcctcgt
    23761 cgaaaatctc catcttggcg tcgccggcca cgactgttcc gtcgccggtg accaccagcg
    23821 gattgatctc ggcaatctgg cagcggtact ccgtgaatat cctgtacagg gcgagggcgg
    23881 cttcgcagac ctgcgcggct attttctttt caaggccggt ttttgcaacc agtgcctcga
    23941 tttgcgaagg cttcatgccg tccaggacat tgatcgaaag tttcctgatg gcttcgggat
    24001 tattttcggc gaccgactcg atctcgactc ccccggtcag cgagaacatg aacaggattt
    24061 cggcggtcga gcgggaaatg gtgcagctca tgtacatctc ctgccgcaca tccagtttct
    24121 cctcgatcag gatgccgcgg ggaagcattc ccttgatcgg gagctttttc agccgctcga
    24181 ttttctccga aaaatccttt gcgttatcgg agaacaaaat accgccggcc ttgccgcgtc
    24241 cgccggtcag cacctgagtc ttcagcaccg cgggcttgtg agcttcaagg aacgcaaggc
    24301 cctgcgccgg ttctgtaacg gcgatgccgt ccggcacacg caggccgttc ttcctgaaaa
    24361 tatccttcgc ttcatattca taaagtttca tcgcaaccgc cctgttcaga ttatttcgag
    24421 actctgcctg cgaaaagaga aaaagacaag cataatgaga gcccgcgaca ctcagcatgg
    24481 gagataaagc tggacataat ggggcctgcc tgctattttg caaacaagta gggacgtaag
    24541 ggcgtagaac agaaaggaga tggtcatgca ggatctaatg attgctcgta aaattacttg
    24601 cacgctgtgt gactacaacc gtctgcgcag tctgctgaca actgcacaac gctacggtct
    24661 tatccggccg gcgcttttgc gaatgcttga agatgaactc ggccgggcgg agatcgttgc
    24721 ttcagaagaa gtgcctcctt atgtcgtgac catgaacact tgtgtctgcc tcacggattt
    24781 agccacgggt gcaagtatgc aatacaagct ggtatatcct gacgatgaag atattcggca
    24841 gggcagactc tctatcttgt cagagctcgg cgctgccatt attggcttct ctgtcggcga
    24901 taccattgaa tggaattttg cggacggccc ccggtatatt cgcattgata tgattctcta
    24961 tcagccggaa gctgtacacc actttgagtg actgcaggag tggcatagcg cgcaatacgg
    25021 tctcttcctt gcgccattcg cttgattgtc tgatttcggt tgatatatcc gccccgcgcc
    25081 gctgtgtcga attgaattgc atttgacgct tgactgtgtc attagttcta tgccgccagc
    25141 agcggcggct ggagccgttg acgggaaagg aatgcgacat gagcaaaaca ttgataatgg
    25201 atgtgttcgg gatgatcggg gggctgagcc ttttcatatt tggcatgcat gtgataaccg
    25261 gcgggctgcg cgcggcggcc gggccgtcct tgcgcacagt tatcgggcgg acttcaagcg
    25321 ggcctttgcg cggcgctttg ctgggtatta tcctgggatt tctggctcac agcggcgcgg
    25381 ccacaacaat gattgccggt tttgtcaacg cgggcctgct gggtctgcag gcggcggttg
    25441 cgcccatcat gggtgcaaac attgggactg cgctttcaat gcaattgctg tcgttcaaca
    25501 tcggccagta ttgctgggtg gccattgctg ccggatttct cttgaaaagc ctgcttcccg
    25561 gcgagcggtg caagcaggcc ggtctggcca tgatcggatt cggtctgctg tttctgggca
    25621 tgatcaccat cagcggttcg ctggtcccgc accgcgaagc cctcgcgccg tggctggccc
    25681 atgtccgcgg ggatgcgtgg gcggggcgtc tgtcgggcgt tggcattgcc gcgttgctga
    25741 ccgcaatgat gaccagcagc ggggccatga tcggcctgtg ctttgccctc atcagcgcgg
    25801 ggatatttca atcatttgac cagtttttcc ctgtcatcgc gggcgcgcat atcggcacct
    25861 gcatcgttgc gttgctggcc agcctttcaa tgaatatcga ggcgcggcgt gcagccgcgg
    25921 gacacctgtt tttcaatatc ttcaatgtcg cgctggcgct tgctctttat ccttttatcc
    25981 gcgccgccgt cgtttggagc gctcaggagc cgctgcgcca ggctgccaat ctgcacctgc
    26041 tgatgatggc gacgggcgcc ctgccgctgc tgccgttcac ccgggcttgc gcatggttgc
    26101 tgaggcggct ggtgctgccg aggtcgccca tgcccgaacc cagccatctg aaaccggaga
    26161 acctcagccg tccggaacag gccatccgag ccaccattct cgaggtccgg cgaatggcca
    26221 ggatatgcat tcaggggatg accatcaacg gggaactgat gctggattac cggccggcga
    26281 cccggcgcgt cctgctggcc aacgaagccg tcattgacga cgtcaagctg gcggtggggg
    26341 attatcttga ccgcctcacg agctatgcgc tttcgtaccg tcagacgctc ttcattcagc
    26401 atctggaccg ctgcatgaag gatatcgagc gcattggcga ttatcagacg gccctcgctg
    26461 ataccactgc cttgcggtgg caccggcgcg aagcgcaggt tccggaggac atcttccgcg
    26521 cctggtttga cctgttttgc gccgcccagc gggttatttt gctgctcgag cggtgcctca
    26581 atccggatga cgcgagcttc caggaaatgg catcgcacct gctggaggcc cgtgaccgtt
    26641 tcagcgcttt gagcgcacgt gacaagagcg cctttgcgct ggctgcgcgc gaccggcgca
    26701 tcacgggaac agccgcctat tatctgcacc gctatcaggc agacctcgac cggatgatgg
    26761 ggcatgtgcg atccatttcc gtatcgctca gccagccgga attttgggtg aggcggtcaa
    26821 agctggacag ggttgaaccc tcaatcaggt cgggaaaaca ggccgccaga atagatgtga
    26881 aggactatct cgcgcggctg tacagcgacg aggtgattga gtggaaaccg gacgagacgc
    26941 tgggagaaat cgagtcgcgc cagatgccgc ctcctccgcc cccggaggac actcatgtgt
    27001 ttgccacgga ctgatccggc ggtttggcat tgcatttatt tctgcatgat ccaacatggg
    27061 gccatatcac gtgaaggatt ggatcatgaa gaaagataat ccgagtctgc cgattgaatg
    27121 gatcgagaac acacacgcaa agaagcctgg ggattgcgag cccctggcgc gcctgctgcc
    27181 ccccgggatc acgggcgtgg cgcgccagtt tcatcgtcag ctcgacgggt atcgcatgag
    27241 cccgttgaaa agcctctcgg ggctggcggc catgctgggc atcgggggga tttgggtgaa
    27301 ggatgagtcc cagcgcctga gcctcaactc attcaaggtg ctgggcggat cgtttgcgat
    27361 ctatcagttt ctcaggaagc ggctgggcat cagagaccgc gaacttacgg tttcggagct
    27421 gaagtcggac aaggttcgtt cgcaaatcgg agacatcacc ttcgcgaccg cgacagacgg
    27481 caatcacggc cgcggcgttg catgggccgc gatggagctg ggtttcaagt gtgtggtcta
    27541 tgttcatgaa cgcacctcga agcctcgcat ccgggcgatt gaggaatacg gcgctcaagt
    27601 cagggtgatc cccggcacct atgatgatgc ggtccgcatg atcacgcgcg atgccgcaaa
    27661 gaatggctgg cagatcatct cggacacttc atgggtaggt tatcaggaca tccctctctg
    27721 ggtcatgcag gggtacacca cgctgatggc agaaattcag gaacagctgg ccgggcaggg
    27781 gatcactcag cccacgcatg tgtttgtgca ggccggtgtg ggcgcgctgg ccgcgtccgt
    27841 gatcggctac taccaggggc tgttcgggaa aaagcgcccg gtcaccgtgg tggttgagcc
    27901 cgacagggct gcgtgccttt atgaatccgc aaaaatcggc gacggcaagc cgcattcagt
    27961 cggcggcgat ctgaacacca tcatggcggg gctggcctgc ggcgatccga gtccgattgc
    28021 atggggtata ctcgaccggt gcgctgattt cttcctgtcg tgccctgact tcgtggcggc
    28081 caaggggatg cgggtgtatg gagtcccgct ggcggatgat ctgtttattg tctccggcga
    28141 atccggcgca gtgacgctgg gcgcgctgat gttcatcgca gaacatgccg gcctttccgg
    28201 cctcaggaaa aagatgaagc tggattcctc ctcgcaggtt ctgctgatca acaccgaagg
    28261 caacaccgac ccggaacatt tccgccgcgt cgtttgggaa ggcggcgacc ctgtgccgcg
    28321 ggaattccgc catgaaccgg catgacggag tctaatgcta tatcagacgc ggattcaggt
    28381 cagacgattg atttttattg agcagaaggc cgccatgaaa cggcacacag aaaactgcgc
    28441 aaaggagaat aagaatactt ggagaatgat cttgttttga tcgggctgcg gtgcaaacaa
    28501 tgagttccgg aaaggcgggg ttttggtatg ccccggatga atataatggc ctccgaagct
    28561 cagttggggt gaaacatgaa caattgttat caacatgtcc ggggattaac tgtttctgtc
    28621 cttttccttc tcgtagccat attcactctc gcagaagcac aagccggttc aatcaccggc
    28681 acggtgaccg ggccggacgg gttgacgccg ttggccaata tctttgtgga ttctgcaaat
    28741 gtttcaacat gggactgggg ttcgtcaacc tatacagatg gttctggcca ttatgagctg
    28801 acgggattgg cggcgggaac atatcatgtg actttcagtg atgatggcga ggtttatgcc
    28861 ccggaggtat ataacaacgt tgctggatca gatttatgga acgatggcac ggatattgtg
    28921 gtgacggaaa cggggactaa tacaggaatc aatgcctcgc tggccatagg atcgaagata
    28981 actgggaaag taacaggatc ggacgggttg acgccgctgg ccaatattta tgtcgaatgc
    29041 gtgaataaat cggattcgta ctggggcccg gaaacctaca cggacagcag tggaaattat
    29101 gagctgacgg gattggcggc gggaacatat catgtgggat tcagtgatga tgacggaact
    29161 tatgctcctg aagtgtataa caatatcccc ggtgacaacg tttggaatga cggggaggat
    29221 ctggtggtcc ctgcggcgag tacaatatca aacatcaacg cttcgctggt cactgcatca
    29281 tggatcacag gcatagtgac gggcccggat gaggtgaccg gcctgcctaa tatctatgtg
    29341 caaacgtatc gctggaatgg atcctggtgg agtttggctg gcgctagcta tcccgatatt
    29401 gacggatact atgaaatcgg gggcttgggg gcgggaacct accgcgtgac attcattgat
    29461 tggaacgggg attatgtcag tgaagtttat gacaacattc cgggtgaccg ttattacgat
    29521 tcgggcgcca atatcgttgt tggggagggg agcgtaaact cgggcattaa tgcttcgctc
    29581 gccattgcgt catggatcac gggtacggtg gctggttcag acggaatcac tccgcttgaa
    29641 gacattgatg tgcaggcata tcaccggtcc ggtcctgaat gggtttatac gggatataca
    29701 accacggata cggatggcac ctatgcgatc ggtggactga tggagggctc ttatcgggtg
    29761 aacttccatg attggagcgg aacctatgtt cctgaagtat acagcaacat gcccggcaca
    29821 gatgtatggc gctatggcac agaaatcagt ctgccggcct ctgttacggt ttccaatata
    29881 gatgcgttca tggacgaata tgccagttta gggggactcg ttgcacaggc ggatgggctg
    29941 acgccaattg cgggtgtatt ggtgcaaatg ctcgggctcg gaaggcttga tggctgggtg
    30001 ttctcggcgc gtacggatgt gaccggagca tatttttgca gtagcgtgta tcctggagat
    30061 tatgcagtgc gcacagtgcc tgcggctagc attggctatt tgggtgaatg gtatgacaac
    30121 aaaggccttt atgtccccgg gcaggaaacg cctccggtgg gtgccgatgt ggtcacttta
    30181 accagcgggg aggaccgcac cgggattaat tttgctcttt atcctgcggg tcgcgtagcc
    30241 ggcacggtca ctggcaatgg catacctctc gctggatgcc cggtgaaagc caagaacgga
    30301 acgtatggac tggtgagaaa tgcggcgacg ggcgatgcag gaacctatga attgatgggt
    30361 cttttgcctg gatcctatac gcttaaggcg gaggccgatg aatacaagga cgagtggtgg
    30421 tcagatgcgc tgcatgaaga ttctgctgcg ctcatagcag tagccagtgg agatgatttg
    30481 tcttatgatt ttgatctcat gcccggccag agtcctgcat tggttgatgt ccgctgtgat
    30541 ccggaagtga atgcggtcgt ctatgtagat tactggccca cgaccaacac gaccccagca
    30601 attgtggata ttggcaatac tgtatcacga cctgctgatg ccggggggtg gggtattgct
    30661 tctcacacag tctctatcaa gaaagccgga gtaccccggt ctgtgcccca gatcgcatcg
    30721 gctgttgaag ctgaaaccat tacgatgaca tttgatgtgt cgtccgatga tgcgggcagt
    30781 gttggtgttg atacaacgcc ggcgggatca caggtctata ttgactatgc ggatgtggcg
    30841 gatggtgtta cacctctggt ggtagacaat cttgccccgg gcactcatac gatattgttg
    30901 aaacagcagg gatatctgca gtcgcggcca gtcatggtgc aggtcggggc ggacctcaca
    30961 aatatgattg tcgtacctct cgcgcctgta tcggagacga atcgaatcat cgctgaagtc
    31021 cgcagcattc cgcctcatgc tgaaatatac gtggattatc tttcaggaac gaatcttaca
    31081 gatgcggtaa ttgactggat ggatcctgcg tcacatgccg gtgccggctg gcatagcgcg
    31141 tcgcacgtca ttttgttgcg caaggatggg tatcaatcca gttcttctta ttatccgcca
    31201 gagggcacca acgatgccgg attactggtg gtcagcttgg ttggagatac caacgtcctg
    31261 gtggatttgg atcatgacgg tatgcccgac cagtgggaag attcctatga tttgacgaat
    31321 ggatgggaag tccaaatctc cgacggcgat tacgatggtg atggggtttc caattacggt
    31381 gaaatgatcg ccggaacgca tcccgggaac ctgaattcgg tgtttgaaat gagcgagttt
    31441 gatctttcct cgcctggcaa caacgaagaa gtgacgttca tattcagtac ggtgcccggc
    31501 cgcagataca tcatccaggg caaagccaat cttgggaaca attctgaatg gatatatttg
    31561 agcggcatca tcattgccac cgattacgag acgtcgtaca cagcttcaat ggcggggatg
    31621 cggtttttcc gtttacttgt ttgggctccc tgatgatctg aagcagtcgg cgtatacata
    31681 cagatgcaaa atgaaagtca tgaaggaata tccgatagcc gttttgtagt tcccaagaca
    31741 agtaattgga cagccataat ctgcggagtt ctgcttctgt ggccttcgtt atctcatatc
    31801 tctgcgtctt cgattgcgtt aaactatttc gataccccta agcgtttgct gtggtcggtt
    31861 atggctttgg tactggcgct ggttgcacga tcggaagggt gtcgccttgg tgcccgatct
    31921 gcatggctgg cccttggact tctcgcatgg atgattgtta ggaccctttc ccggcccgct
    31981 ccggctactg agatcgaggt gctgttcaca tggatgcttc cactcctgat gctgattctg
    32041 ggggcaggta tcgaaaactc aaagggtatg cgcatctttg gggcccttct cgtttccgct
    32101 ggcataattc aagctctttt gatggtgctg cagcggtttg gcctcgatcc cttgttccgt
    32161 caaactacat cagcaatgga ctatgcgccc ggacgcatga tcgggaccat cggctatcat
    32221 aatcaggccg ttgatttcct ggcgctttcc gcgagcggaa tatttctgct gagcaaatcg
    32281 tcgagattga agctgtgttt catgcttttc atgttgttcg tggccggcct cacagccaat
    32341 cgcggcggga tactggcgtt tgtctttgca ctcgcagcag ttcaatcctt tgctgtatgg
    32401 atcaatccct tatggaaatt gcagaaaaaa gcattgatca ctgcatcagt gattctgggt
    32461 atttgcagcg tgcttggcgc gatgactttg attccggaga cgggcgatcg cttcaaggac
    32521 gcagtaaaag attttcgaca ttccccggca gttcagtccc gggttctgat gaaccgcata
    32581 ggatgcgata tgttcacaga gaagccatgg atcgggtggg gagcagggga atatgcgcac
    32641 cagtatcttg atcggctagg gaatgttttg ccggaagaga aaacgcatga aatactcggg
    32701 aatgtcgttt tcgcccgcga aacacacaat gactatgttc agttcgctgc cgagttcggc
    32761 cttctgggat ttgcaatcct tgcagctctc gtctgttcaa tcgtgatttc cttcattcgg
    32821 gcaggaaatg accaatcgga tgcggtcgca accgctatgt ttattcttgt atacatggcg
    32881 gtggcgtcat tgttttcatt cccatggcaa acaggcatgg gcgggcccct ggcgggattt
    32941 ctgattggct ggctgctgcc gcagaaagaa acttcaaaaa acgaagtttt gcgaatatgg
    33001 ccaaaaatat caaaatcggc gctggttgcc ttttcactga tcctggttgc atggtccgca
    33061 gtggattcat ttttcaacag gtctgttccg gatatccttg ccgagggcca ctcggaaaga
    33121 gccgcccgca tgctgccccg ttttgcttat cggtaccggg cgcttgtggg cgctgcctat
    33181 gctgtggcgg gagattatga agccgctgaa aaagaactga ctgcggctca aaaaggctat
    33241 cgcgacatcc ttctgtggaa caatcttgga aacgtctatg ggaaattagg gaaatgggat
    33301 gaagctgtga ccgtctacga gaggtgggcc aggtgcggca tagactatga tagtgcgctc
    33361 cgcaatcttt ccgttgcgca ggaacaggcc ggtacacttg aagaggccgc ggagacgctc
    33421 gcccggcgca tatccctgtg gcggaccacc ccggatgaag tcaagcggct tgcggtgctg
    33481 cagatgcaag ccggatatct tttgaaagcc cgggaaacgc tttttcagta caggggtaaa
    33541 tggaagtctg cggatccgaa aacagtcgcc gaaatagaga atctgtcggg agcgatctcg
    33601 cttaatcttg aagattacgg ggaagccgag aggctattcc gcagcgccct gcagaaatat
    33661 cccgggctgg aaagcgcacg acgcaacctc gatcagttgc gggtcatcac ttcagccgcg
    33721 gattcaggtc ggtgaggtgg tcatagaccc gccgggccgg ggagccgggg aaggtctggc
    33781 ggatcatttt cggatcgagt tctgcgctga tgatgtcctc ctgcgcggcg gccagcgcca
    33841 tgatgtttcc ggccgggccg acaatcatgc tgtgtccgta gtagtgccgt cctgcggcat
    33901 caaatccggt cgcgttcgcc gccgccagaa agacatggtt gtcaaatgcg cgcgcgcggt
    33961 tggtgagcga ccagatttca tagttccgca gcagagccga cggccggacg atgatctccg
    34021 cgcccttttt cgccagcagg cgcgaggcct cgggatagtc gccgtcaaag cagatgatca
    34081 ttccaatctt tgcaaacggt gtctggaaga cccgcatctt gctgccgggc gtggtccacc
    34141 cgccgcgctc cgtgctctct ttgtcgaacg gatgcgtctt gcagtatttc ccgataatct
    34201 ttccgtttga accaatcagg atggcggagt tgtatatctt ttgaccaact ttctcatacg
    34261 tcggccagac gatgtgcagc ttcagctttt ttgccgcatc gagaatcgga gcaatctgct
    34321 tcccgggcac ccggtctata actttgagaa atgccttttc ggacatgttg ggcgtgaaac
    34381 ctgtcgtcac ggattcctga aacaccacga ggtccgactt atgttctctc tttgccttgt
    34441 tcagaaagta aagggcacgc tcggtgttcc cggtcacatc cagcggacgg ataaacatct
    34501 gtacacagga aataacgcac tttttcattt cagtccctcc ggaatgtgat agaggccctt
    34561 cgaacatttc acaaggtctt cgaggctgtg cgcggccagc aggtccctgg tggcggaggc
    34621 cgggtcaatg cccaggtcct cgacggtctg gagaatgccc ttcttccgca gtgccgcctt
    34681 gtcctccggg gtggatgttt taccgacttc ggtttctcct gcgacggaag caacggcttt
    34741 catgcgttcc ttcggcgagg cgcatttcat caggttggca atgccgcact cggtgacgat
    34801 atgggtgatg ctgtcaccat agagcatgac cggcggggtt tcaaactttc cgctgcggta
    34861 taatttggcg gcatccagtt cctccacaaa aaccggaatg gcctttttct cgctgacggt
    34921 gggcgtcatc tgcacgacaa tcttgcggcc ggatttgtag ccggtgtatt ttttgaaggt
    34981 cttgttttcg cgcgagcttt tttcccacgt gggcgaggaa tgcctgcgtc cgggaggagt
    35041 gcttccgaga ttgggggcgc cgccgaaacc ggcgatcatg ccgtcaatgg ccgttgagga
    35101 gtttccgcag cggtcaattt gaagcgtgga gcctacaaag cagtccactc cgtacaaacc
    35161 gaggagctgg gcgttgagcc gtccgctccg catggagccg tccgggccga tcgagaaaat
    35221 gtccggatgg gccttgcagt attcctccat gcccggttcg ccgccgaagg aatagatgcg
    35281 ttccacaaag ccgctttcga tggcgggaat cagcgtgggg tgggggttca gcgcccagta
    35341 ctgacagatc ttgcccttga ggtgcccggc aa
//
LOCUS       DAOQWR010000008        33931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1495_length_33931_cov_5.585902, whole genome shotgun sequence.
ACCESSION   DAOQWR010000008 DAOQWR010000000
VERSION     DAOQWR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 33931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33931
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_33931_cov_5.585902"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKK36_01345"
     CDS             complement(<1..304)
                     /locus_tag="PKK36_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93235.1"
                     /translation="MKCSRFRKETLDAEQLTPECLAHAQECPDCRALFEQGLALRSLL
                     ALRKHERIPGDSLDCTRRAFRLAAAESCEPSASGLALVLRYGLAGAFMMLLVINFSI"
     gene            complement(301..909)
                     /locus_tag="PKK36_01350"
     CDS             complement(301..909)
                     /locus_tag="PKK36_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR93236.1"
                     /translation="MVETEKDNAAQEPGPPDAELVARAQNGDAAAFEELIGRWHEKIY
                     ALVYGMTSNREDAQDILQEVFIKAYRSLRRFRGTASFYTWIYRIAVNHTINYRKKRNR
                     RAALSLNEMDDSIEKDPAFVRLQAHQGPRYETSVSELHEKLNKALQTLSEKHRTVVVM
                     HDMEGIPHEEIARMLRCSPGTVRSRLFYARRLLQQELKDYMP"
     gene            935..2299
                     /locus_tag="PKK36_01355"
     CDS             935..2299
                     /locus_tag="PKK36_01355"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HNR93237.1"
                     /translation="MNSLTKFMRMGIAAAAIAAPRGAAEEQPSPNSVFVQNMYTRQMA
                     SAAGSSNFLVRPGIWADRTAKKVSFWAEATGITPGEPVEFYVISPKSGHAYEALAVSF
                     AMPGDVHAALEFIGMKPGSVVDYESLRIWPKGERVFLTFQWEQDEGVLVRRPAEQLLV
                     DADTGATLPEKGFCFTGSAVMDGEYAADAEEPGSIASDFNDRATVLDVPFKRDKSGVY
                     RRFSANPDLQIPRGRLVQIDIEPEYMDGRLRIADITLSVSAPEGAGWKDLICSAGLSG
                     APTSTTPEGLMEYLQELIENGRTPHVDLRFSPDVQLGHAVELCRFLDLLCTDNGIRME
                     PPSKGQLFYKAFLPQPEFRAREDRPSQPWEIHAGGTNGLKLVQISEEWPDDQPKPDLK
                     IAEFSAANAAGALDIIREKGAGIPVAVIFAPETAPYGELVSLAGALREPFPTVYIFSG
                     SPED"
     gene            complement(2307..3986)
                     /gene="rpiB"
                     /locus_tag="PKK36_01360"
     CDS             complement(2307..3986)
                     /gene="rpiB"
                     /locus_tag="PKK36_01360"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNR93238.1"
                     /translation="MKIAIGSDHGGFEAKEHVKQYLEARKIKVIDVGCWSRESVDYPD
                     YAAEVARRVSCGEAKQGIVICTSGIGVSITANKFPRVRAALCGTPHLAEMARKHNNAN
                     ILAMGASVTPLDEMDAILDAWFAAEFEGGRHERRVQKITGYMATLTDPVALYDSDPEI
                     CSLIVSETRRQKENLELIASENYVSRAVREATGSVMTNKYAEGYPGKRWYHGCEFVDE
                     AERLAQDRAKELFGAEHVNVQPHSGSQANMAVYFSVLKPGDTILAMSLAHGGHLTHGH
                     SVNFSGRFFNCVHYGVDRETEMIDYDEVERLAAEHKPRLICAGASAYSRIIDFKRMRR
                     IADSVGAYLMVDMAHFAGLVAGGCHPSPVPYAEFVTTTTHKTLRGPRGGMILCQSRFA
                     QDIDRQIFPGVQGGPLMHVIAAKAVCFHEALQPEFKEYAKQIVRNAHALADALRAQGV
                     RIVSGGTDNHLMLVDLTPVNMTGKDAADTLDRAAITVNKNAIPFDARSPFVTSGIGIG
                     TPAVTTRGMKEGEMSAIAGLIAEALGKAEHPKALALLKKKVIELTAKFPVP"
     gene            complement(3983..5071)
                     /locus_tag="PKK36_01365"
     CDS             complement(3983..5071)
                     /locus_tag="PKK36_01365"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNR93239.1"
                     /translation="MKTPQIHRTDPADIPSGVIDEAVRVLRAGGLVILPTETVYGVAA
                     MPPVPGAENRLYEAKGRDSGKPVARLARGIEDVEAAGGRLNAAARRLANRFWPGPLTL
                     VLPQNGGTCGYRVPDHPVPLALLDACGCVLAVTSANLSGQKDAVTAAEALAALGDHAD
                     LVLDAGPARGQNPSTVVDVTGEEPRILREGAIPGAEIAAALVSGPAEKRILFVCTGNT
                     CRSPMAEFLLRARLGENSGWAVESCGIYAVDGASAFENAIDVMQELNIDIRPHRSRQV
                     TAEMVESAALIVAMTEGHRRELLNQFPAARGKVFLMHEFGTEGCVRDVMDPAGGAKTV
                     YRKTRDEVDSAVADLILFMARNFERTGK"
     gene            complement(5049..6233)
                     /locus_tag="PKK36_01370"
     CDS             complement(5049..6233)
                     /locus_tag="PKK36_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR93240.1"
                     /translation="MKDLKRTILAGILCAAAAASVCGEEAPATAVQTLVRAMDLLHER
                     GVIAGPERLEAGMLSNLVHAADPGGVLLGPEDLSCREQEARGQWGMPGLHFMVTNGSF
                     AIAAPAPEPCRPGDVLELVDGQGIRGLPEEEVWRLLAGNTSEEARLTVRREGSTEHEI
                     VLPRIAAPIPPVAASEKISPETGYIKLYGCHPGTADALRPILQEWKTSGVYGVVLDLR
                     SAAGASREETCRILSQFFPSERELFRMVPLSGDPEICLSEGASPRTLPTMVLVDETTA
                     GGAELLAAGLQSSETGTMILGRRTAGDFLLREVLELNGSHAMLVASRILKFPDGREYR
                     ANEGLTPDIVVEGRPSGATDQAEEEIHPRLKNDPALRRACDLLQGLRVLKIKFREDST
                     NP"
     gene            complement(6230..6613)
                     /locus_tag="PKK36_01375"
     CDS             complement(6230..6613)
                     /locus_tag="PKK36_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNR93241.1"
                     /translation="MHFDRGAWALQKERFATDVPAPPPPFEVHPLAEVVPDVMKKIGM
                     HGRLHEAAMQEEWPRLVGPQLADHTRPGKLDHDVLTVYVDHSAWLSELSRYGRKQIME
                     KLKARFGAGCIKSIHLALDPEGGKK"
     gene            complement(6673..6882)
                     /locus_tag="PKK36_01380"
     CDS             complement(6673..6882)
                     /locus_tag="PKK36_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HNR93242.1"
                     /translation="MSAQDSGSGHGDPEQWVRQTRLVKDWERRKQDILAHKWYESERA
                     GRDVGWDHATVDWLIRHGITPKKQT"
     gene            7041..8093
                     /locus_tag="PKK36_01385"
     CDS             7041..8093
                     /locus_tag="PKK36_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93243.1"
                     /translation="MKKRTVRFFAFAIISSCALLPARAQLYAGRVVDPLDQDFSITLK
                     PGLITSVSGYVKETKRAYTDIPGYTQDYGRFLQNYDLEELGFDESYGALGLGIEKRWR
                     FVTFLLDVSYFEASATATAKDEPFAIGVEEVKFEGKSYDYMFIPQGSSADVDLQAGSA
                     ELGLLLTPFCISTEEGFDFSPWLRAGLLAMGGSYTIDAGPAQGVRTYEYHPYPYVIGG
                     TGEGWAGIVIPEFGIGGEMRIWLNPEGTKIVCQGNYMMLEWAGRSSSLGADSRNDKDI
                     DLSYKSIDLTVMLEHPLSEGVDLLLGATFRHLETDATIEARHKSAEEQDAAREKYDKE
                     ASIEMNTFYLLAGLRF"
     gene            8216..8398
                     /locus_tag="PKK36_01390"
     CDS             8216..8398
                     /locus_tag="PKK36_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93244.1"
                     /translation="MERFHRIVPVLAAALALCAHCALGSGFAIVEQSASGIGSAFSGA
                     GTSIGDASTINSIRPA"
     gene            8395..9513
                     /locus_tag="PKK36_01395"
     CDS             8395..9513
                     /locus_tag="PKK36_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNR93245.1"
                     /translation="MSRVPGSELTLGGHMIMPTFEFDNSGSVILPAGVPVSGGDGGNG
                     GVAAFVPNAYYKADLCEKTAWGIGITAPYGLTTEYNKSWVGRYHAIKTELVNMNVNPS
                     VSYKISDKIAVGAGVSASYVEADLTSAIDFGTILGVAPQALDGRARVEGDDWAAGYNA
                     GVLITPRPECALSLSYRSKIEHKLEGDASFQVPGPARALQAMGMFVDTDGEAELTLPQ
                     SASLGMAHTFAEKVTVMADLTWTGWGDFDELRVKYGSSQPDSVTDESWDDTWRYSLGL
                     QYQQTDKLALRIGGAYDETPVPDANHRTPRIPDADRIWLSLGAGYQVSENLSVDVGYT
                     HLFINDSRVQQMGATGDYLNGSYEGSVDIASIQANWKF"
     gene            9590..11062
                     /gene="glpK"
                     /locus_tag="PKK36_01400"
                     /pseudo
     CDS             9590..11062
                     /gene="glpK"
                     /locus_tag="PKK36_01400"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
     gene            11131..11670
                     /locus_tag="PKK36_01405"
     CDS             11131..11670
                     /locus_tag="PKK36_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93246.1"
                     /translation="MNGAAFVAGMFILLGTVILLASLIRTQLLFEAIPLILSKSRAPV
                     VRMLRLYRHLLLFIILGYLVVIAGLAGELWNLSTAVSGAVFFFFSVYALLCSRLQSTL
                     FKELEKTLHGLLPICAACKKIRTEAHKDEPVWVPIEVYISERTDAQFTHGYCPECAES
                     MLSEFRSGKKSKKSTPATE"
     gene            complement(11651..12061)
                     /locus_tag="PKK36_01410"
     CDS             complement(11651..12061)
                     /locus_tag="PKK36_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR93247.1"
                     /translation="MEFDAAVSAMMACDLFSELSESEQGGLLMRATTRHCGAGTAVYE
                     KDQKSGSSFALIADGCVNILGQDGAVLETVGMGSIIGEIGPVSPQKKRTFRVEACGDV
                     DLLEWYLSDIEEKYPAVLKRLRDLAWDRITQWRE"
     gene            12156..12701
                     /locus_tag="PKK36_01415"
     CDS             12156..12701
                     /locus_tag="PKK36_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR93248.1"
                     /translation="MNFLDLANRRHSLRKYAATPVPRAILDRCLEAARLAPSACNSQP
                     WHFIVADREPLRTQLAAAAFSGLYSMNAFAKSAPVLIVAIRTSSRASAAIGGAVRGIQ
                     YGLIDVAIACEHIVLQAAEDGVGSCWLGWFNEKAVRKLLGISRTNRVDTIISLGYPAE
                     ETGRPKIRRELDAIRTFNPEP"
     gene            complement(12714..14675)
                     /locus_tag="PKK36_01420"
     CDS             complement(12714..14675)
                     /locus_tag="PKK36_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93249.1"
                     /translation="MGFALQPPERVNYQGRLIEGTNLVNGSRQIIFRLFDEVSAGIEL
                     YGETQTVSVVDGLYSTAIGLSNTVAGSLLAALKGSSCFLEVEIGGTVLSPREEIAAVA
                     HALTAVSKAGDTMTGPLTINAGAGEGILILSEGSGIAIGNSASAEGGEERIAIGHNVT
                     NEVDHTAAVRGTLYLDGGTNIYYRSTAGTGPWQELMVEEDPVWLSEKGAYATGSPVYA
                     EADPVWTAEREAYATGTPLYVFAETDPVWTAEKAAYATGMPVYAEADPVWIAEKAGYA
                     TGTPLYTYTETDPSWASSSNDVEIRILNIETARVSKIGDEMTGALVINVSSVTGIVIG
                     SSGANIAIGNTANVAGGGLAVGDRANAAVNGTALGYMAFGAYVGTALGRTANGSSSGA
                     SVGQAANSFQSGAAIGFEADGSSWGAAMGRGANAKNSGISIGHFSHGREYAVAIGRDS
                     YAVQTNISIGYAAKCWAGRNRIAMGSGVVNDTDDSAQIRGSLYLDGGTGLMYRSTFGS
                     GAWSVKAFTIDHPLDPENRVLRHYSVEGPKVWNVYAGNAQVIGGTAVVELPAYYSALN
                     LAGSEVYSFTPVGVAAVCYVKQKAAGNRFVIGADQDCEVSWTVKAVRNDPAAREDLLH
                     RPVEQLKADIRSLPPISEAEQQNTTAISP"
     gene            complement(14771..15412)
                     /gene="thiE"
                     /locus_tag="PKK36_01425"
     CDS             complement(14771..15412)
                     /gene="thiE"
                     /locus_tag="PKK36_01425"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNR93250.1"
                     /translation="MKPFGLYLVMTDPLAGYEECARAAVDCGVRMLQLRMKKASDVAL
                     LDMAVKIRAITQNSETLFIVNDRVDIARAADADGVHLGQDDMPLQEARARWPAEGKIF
                     GLSTHNLGQACAAVMQNPDYIGVGPVFPTPTKEIPDPAVGLEGLRKILQAVSLPAVAI
                     GGITPENLPDVLAHGAQNFAVVRAVTKSPDPRAAIQRLLRTAGIEQILWPRVV"
     gene            complement(15414..16526)
                     /gene="thiH"
                     /locus_tag="PKK36_01430"
     CDS             complement(15414..16526)
                     /gene="thiH"
                     /locus_tag="PKK36_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNR93251.1"
                     /translation="MDNPSQYWSLAQAINTAASRADNRQIFSAMGAQRPGPRELALML
                     QPQARAHLEQMAARAKSLTARHFGNTVSLYAPLYLSNYCSSGCVYCGYASDRKQPRRK
                     LPFDAVAREMDALKREGFEEILLLTGERTPQADFEYVQACVRMASELFPQVQVEAFPM
                     TVDEYRKLNLAGCVGVTLYQETYHPVVYDMMHRWGPKKDFAGRVSAPAHVLEAGMRTV
                     GMGVLLGLSDPVVDSIALYLHAEEFRKKYWRSGIAISFPRIRPQEGGFQPAHPVEDRL
                     LAQIVFAFRIVMPDVPLVLSTREKPSFRDGMAGVGISRMSAASRTTVGGYAGAPAEDR
                     GQFEVSDRRSVMEICDMLQAKKIEPVFKNWDAVYTG"
     gene            complement(16526..17296)
                     /locus_tag="PKK36_01435"
     CDS             complement(16526..17296)
                     /locus_tag="PKK36_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNR93252.1"
                     /translation="MKPLVLGDLEFQSRLLLGTGKFSSSAVMLEAVKASGAQIVTVAL
                     RRFNRERPSDDLLGPLMTLKNITLMPNTSGARDAAEAVKAARLARELCGSRLIKVEIH
                     PNPHHLLPDPIETYEAAKILAADGFIVMPYMPPDPVLAKRLEEAGCASVMPLGSTIGS
                     GNGIAAGGLLDIIINNSNVPVIVDAGLRAPSEAAAAMEAGCAAVLVNSAIAAAGNPSA
                     MAQAFAAAVQAGSAAYEAGLMARSTTAAATSPLTLFLG"
     gene            complement(17299..17499)
                     /gene="thiS"
                     /locus_tag="PKK36_01440"
     CDS             complement(17299..17499)
                     /gene="thiS"
                     /locus_tag="PKK36_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNR93253.1"
                     /translation="MMKLVVNGEEIESSGSSLPDLLVQMGASPKRVAVMVNGTVISAA
                     ERQAVTLRAGDRIEILTFAAGG"
     regulatory      complement(17529..17625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17741..18784
                     /locus_tag="PKK36_01445"
     CDS             17741..18784
                     /locus_tag="PKK36_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNR93254.1"
                     /translation="MNSGDKIEQAAIPMRHIGPLKMSGPAVEGEFMLPLATYESPLWP
                     SVGRGARVSMQTPGLCAVITDDRMSRSVLVQAATAVEAWKIASALQGRHAEIAAAAES
                     SSRFARFLDLHHQVVGNLLYLRLEMQTGDAAGHNMVTNAAEAFLNWILQQYPQLKYVS
                     ISANFCTDKKATAVNGILGRGRGVICETVIPREVCAKVLRTTPEAVADLNLKKNLIGT
                     SLAGGIRSANAHFANMLLAFYLATGQDAANIIEGSQGYTLAEVQNNDLYFSVTIPNII
                     IGTVGNGKDIEFVRKNLEALGCLEKRPAGENARRLAALAAAAVWCGELSLMAALTHPG
                     ELMSAHRKMERRK"
     gene            18781..19818
                     /gene="fni"
                     /locus_tag="PKK36_01450"
     CDS             18781..19818
                     /gene="fni"
                     /locus_tag="PKK36_01450"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020285084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNR93255.1"
                     /translation="MNESINQRKLEHLKMAADESADRQKRYWDAIHLTHRALPEINAA
                     DVDASAEFLGKNLTFPLLISSMTGGDDQQILRINRHLAAAAETTGVALAVGSQRVMFD
                     NPEAVESFRLRALAPSVPLLSNLGAVQLNHGFTEEHCQKAMDVLEADGIYLHLNALQE
                     TVQNGGDTNFAGLAEKIGHVAGKLNKPVLIKEVGAGLGPADVEILMKQGIHYFDVAGT
                     GGTSWSRIEQFRRVCEDAPDLGFLYQDWGWPTPLALRKLAPLRDRITLIASGGIRTGV
                     DMAKAVILGASLCGIARPFLKPAMVSAEEVVKVIRRLKKEFIAAMFLTGMNSVSKMRG
                     NLSLVRTDDPE"
     gene            19819..20826
                     /locus_tag="PKK36_01455"
     CDS             19819..20826
                     /locus_tag="PKK36_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93256.1"
                     /translation="MKARAPGKLILSGEHAVVYGRPALVTAVNRYADCTIHSEDRDVF
                     RFLLTGLDKELSLSAKSLAGLHRTLNERYERFRLGELPIRDVHGRPAELTAYALACLL
                     QHTGITPAGGLTFELESSIPLGSGMGSSAAIVLAVLQAADSLLRGGLERNVLFDLAMM
                     SERLQHGNPSGADPYVCLHGGLHRFQKEHSVSLPCPGTAYRLAQTGRPQAGTGECVSF
                     VARHFGTSGIWREFELLEGRIEKALLSGEWGILQEVVTDNEALLERIGVVPKRVAAFI
                     RDLNRQGCAAKVSGAGSVSGDDHAGIVWIVSETAPTSLCDEYGYSLIETEGDADGTRI
                     V"
     gene            20807..21733
                     /locus_tag="PKK36_01460"
     CDS             20807..21733
                     /locus_tag="PKK36_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNR93257.1"
                     /translation="MARASFRVSAPGSLMLLGEHAVLHGRRSLCGAVDKRMEVKLTPQ
                     KSGTLHIISALGSYESSLRDLAPSRRFSFIIHAVGKQRDRLPGGLRLDVKSGFSSTVG
                     LGSSAAVTVATTAALMEWAGQSYSREDIFREARGTIRAVQGLGSGADAAASVFGGVLV
                     YRMDPLEIRRLTCCPPLTLVYSGYKEPTVKVVQLLEQRRNNDPEYFERLFDAIDQSVP
                     QAAGAVEAQDWPALGKILNGNQEFMSQMELSTPDLDRIIEILRSRPNIFGAKISGSGL
                     GDCAVGIGEIRLRGIEYEQIDVKLSPRGVEID"
     gene            21726..22709
                     /gene="mvaD"
                     /locus_tag="PKK36_01465"
     CDS             21726..22709
                     /gene="mvaD"
                     /locus_tag="PKK36_01465"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNR93258.1"
                     /translation="MTDKEAAQLITGAAKGCPKECGQAYAPVNIALCKYWGKRDEMLN
                     LPVTSSLSLSLPELGATTRISFREGHDQLFLDGVEQSRDSVSARRVSKYLDLFRPDRS
                     TFFHVESESTVPIAAGLASSASGFAALALALNDLFQWNLGLRDLSILARLGSGSAARS
                     IYTGFVLWHAGTREDGMDSFAEPVDGEWPGLCMGLIMVSRREKAVSSRIAMKRTVDTS
                     ALYRAWPAKVESDLKNLRQAIEKKDFDLLGRTAESNALAMHATMMDSQPPVLYWMPET
                     VEEIRKIWRLRDNGLPVYFTMDAGPNIKMLFMEKDASLIREHFPDMEIALS"
     gene            22822..24927
                     /locus_tag="PKK36_01470"
     CDS             22822..24927
                     /locus_tag="PKK36_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93259.1"
                     /translation="MAEHNANKTGDLPELPGQKSSAYRDLFEHMAAASCLDELIYRDG
                     KAVDYRILEVNASFERITGISRARAAGSLASELYGIKDVPYLDIFSRVAKTGEPVRIN
                     DFFPQFGKYLDMIISRPREGCFSTVFTDITEQRKAQEALRISESKLSAALKMARAGHW
                     EYDVESDTFTFNDNFYRIFHTTAEQVGGYQMSSANYARRFCHPDDAAMVGEEVRKAIE
                     SDDPGYYRQIEHRILFADGGVGYMSVCFFIVKDRTGRTIRTYGVNQDITDRKRTEEEL
                     RESEELYRAFINSTEDLAFIKDDAFRYQVVNRACARFFQRTANEIFGSTDAELMTPEA
                     AARCRMTDRQALETGQVVVSEEQVGDQVFETRKFPVRLRGGRVGVGGVIRNITERKIT
                     ERTLKSERDFSTTVIETLPGIFYCCDENLQILRWNRNFERVTGYTAEEISRLSALDLF
                     TGTERNLVALRIREVFEKGESEVTADLSAKDGTRIPYYFTGRVAELDGRRCLAGVGMD
                     ETAVRQARKAGEESKARYDLLFENSLDAMLFTAPDGRIFSANRAACRMFGMSEQEIVR
                     AGRESLVDSSDPELGRLLKERETTGSASGVITMVRKDGSKFPAEISSSIFREENGELR
                     TSIVIRDISARDREDRNIHSQLDELRRWSEVTMGREMRILELKREVNELLSRAGLPPR
                     YSSAGEQNTGASSAREPGA"
     gene            24924..27545
                     /locus_tag="PKK36_01475"
     CDS             24924..27545
                     /locus_tag="PKK36_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR93260.1"
                     /translation="MKKSARICTCGFALAWILLAGAQVICAAEGTSTITSGCEVGYPP
                     FCIVHETGEVDGFSVELLRAALKSMTRDATFQTGTWDEVRNLLIDGKIQVLPLVGRTP
                     EREEFFDFTVPYLTMHGAIVVRMDQTGINDLEDLKGKNVAVMSGDNAEEFLRREDRGI
                     NIRTTATFTDALRELSEGRHDAVVIQRLVAMRLIREAGLKNLRIVNKPITGFRQDFCF
                     AVQEGDRETLALLNEGLALVVADGTYRHLHAKWFAALEMPEKRSIIIGGDHHYPPFEY
                     LDEKGQPAGYNVDLMRAIALAMGLTIEFRLADWVETIEALRDGDIDLIQGMFYTPARD
                     RDFDFSTPHAVNQYISAVHRSGGPPPRTIGELLTQNIAVQTGDAIHDFAAENGLTNLT
                     LVDSQEQALRMVVEQEVDCALVSRLSALHWIQKHGWDELRLSREALISLEYCFAVSEG
                     NRALLALFSEGLRTVEDSGEYRRIYAKWFGVDETKRLDWRIYIRFAALILLPLILLLS
                     AVSLWSWSLRRTVARRTEELRQEVAERQRAGEKIEMLLTESDRARSALLSILEDQKRA
                     EATIRSQTELLHTMLNAIPHPIFYKDTQGRYLGFNRAFEQFYGKTKSELNNTSVFDIA
                     PRELAEIYHQKDLELFGSPGTQIYETKMLDAQGVMHDVVVHKATFNDAGGRVAGLVGT
                     ILDISERKRLESALEQKVEERTRALKRIGTEQQTILNSIRAAIIYKDTRNIILRVNRA
                     AGELLGLPESEMEGRSYADLFPEDAVSQIEDDMEIIRTGAPRFGIEQQLSLPSGGTRW
                     MLMDKIPYRDETDTVIGVIVMGIDITNRKEAEQSLRQRSADLRTMVSAMANRELRMIE
                     LKEEIERLRKQSPEGTA"
     gene            27577..31335
                     /locus_tag="PKK36_01480"
     CDS             27577..31335
                     /locus_tag="PKK36_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93261.1"
                     /translation="MNTEQNRLPVSSEAAKGNADRFLFGALILLMFAGFWTAREIGQR
                     ADRALRNEILLSAGRIARAIHPDRVRQLTFTATDTNNPAHLRLRTQMRAYAEAMGLRS
                     LYSMALRDGKLVFGPESLDPNDPYASAPGTVYENPSPMDFEVFQTGQANVQGPRTDEY
                     GSFVSALAPVIDPRTGEVLMAVGIDLETPMWKTAVRRTQLIVIEILILLFVVLAAGAL
                     AVRVRQRYTVRRHPLLIYAETAVCALILLIATVVAARLTHQAEERERTESFRFLAQAR
                     AAAYSQTFSYLRNELNMLSGFFNSSEHVSRSEFNSYCQPLVDSRLIQACLWIPSVSSV
                     EKDVFTDKARAEELHGFSIWEPGPDGSPAPAAERDIHYPVLYVEPRDDHQRLTGYDMS
                     SDPLRRSAIDAALQTGHPSASDPVTLFAAPGSPRGFVVFQPASNAGQTGTVGMGIVPD
                     AIVQRRAHVSDNAEMTLSASLYHLAPGRPPLWLACSRGCAHPHDRAALRHAIHESMPV
                     FIFGQVFEVLIGPEPEWLATHPLRRWIATLLAGGALTFLFSALMGIAAGRQTRLEHAV
                     WERTAELAKSQDRFSSLFRNLSSSFALHEMIYDRKGRPCDYRFLEVNPAFEKMTGLKA
                     RNIIGRTVREILPETEDVWIERYGRVALTGRPEQFEMFAASLNRHYSVMAYRTGPDQF
                     ATLFDDITDRRKAEEDMRRLQEIVTGSPNVAYLWRAEPGWPVEYISENVRQYGYSPED
                     FTGGRIRFDEVIHPDDFERVVRELEEYSADENLTEFKQEYRILAKDGRIHWVHDDTRI
                     LRDSCGRITHYQGLIVDITALRKAEAAHAESEERYRALIEAQADAVCRWLPDTTLTYV
                     NHAYCEFYGRTREELLGSKWIERVAESAREEILSTYAELVADPKPFMYEHESVAADGS
                     LCWQQWIDTPLFDKTGRLVEFQSVGRDITKRKHAEVELERHRQRLEELVQERTSELQT
                     RVEEAERLNHAMANLLSDIQLAQARLEQSRQELQMSNETKARFLATMSHEMHTPLNSV
                     IGFAEALEDGVYGTIDEQQREPLENIRESAQQLHRLINDLLNITRIEGGVMPFHADSL
                     PIRQVLEECCMMLRAAARSRQQQLSADFRPVPDSLRLRGDVVKIKQIMHHLISNAIKF
                     TPDGGRIEVSGDLVDGTDFGLQGRALRVSVRDTGKGLTADELACVFGLFYQSEAPLTR
                     TQGGTGLGLPIAKEFIRLHGGRIWAESAGPGRGCVFTFVIPTQSCEPS"
     gene            31343..32482
                     /locus_tag="PKK36_01485"
     CDS             31343..32482
                     /locus_tag="PKK36_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93262.1"
                     /translation="MNTNSSSPKIEETGSILVVDDDPRNRTLLHDLLVSRGYAVFEAG
                     GGEEALKMMAENPPDTVLLDIMMTGMDGFEVCRQIKGNPDTAHIPVLLVTALTGREDR
                     LAGIDAGGDDFITKPVDLKEVLLRTRNAVHMKQLRDRVRKSFIELEKMSLMRENLVQW
                     LVHDMKAPLGGVLGHLEMLQMKTVNGPLQVYVDEACAATRRLHEMILAILDVSRLERH
                     EMPLQRSVRDLARIASEAVRQMAFEIGEQKIAVEIAAEPAPAWCDGEITRRVMDNILS
                     NALKYTPEGGRIAVRFTSEDGQTRTDIEDNGPGIDPKYHDMIFEMFGQVQMRREQRTY
                     SSGLGLAFCKMAVEAQGGKIGVISEEGKGSTFWFTLPSNPPENEV"
     gene            32479..33798
                     /locus_tag="PKK36_01490"
     CDS             32479..33798
                     /locus_tag="PKK36_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR93263.1"
                     /translation="MTAKRRILIVDDDPSIRSLFSRFVQDSGYDFRTAASLAAMWKMF
                     RSHSFDAVLLDMNLPDGSSLDCVAQIREQFPHLTIVMITANAELPLAVEAIQRGADNF
                     LEKPVNLPSLSAYLKSGFEAGALRRHKVVRDRKKPPFVPFRGSSSDMRHAWELAGIAA
                     RNSAAVLILGETGTGKGVFARWIHENSDRAERPFVSVNCSGLRGEMLASELFGHVKGA
                     FTSAVSDKTGLIELASGGTLFLDEIGDMDPELQARFLKVIEEKRYRRLGEAEERFSDF
                     RLICATHQDLQKRCRENLFREDLLFRINVFPLTVPPVRKCRSDIPALADYLIKSLAPG
                     REDVITDEALNLLKKYGWPGNIREMRNLLERALLLAQGGPIEARHLPGLSENGAERPG
                     SELNLNHLEQDRIKAAMKQAGGSAGEAARLLGLSRATLYRKLKKMQK"
BASE COUNT         7048 a        10235 c        10067 g         6581 t
ORIGIN      
        1 tgatggagaa gttgatgacc agcagcatca tgaacgcgcc ggccagcccg tagcgaagca
       61 ccagcgcaag accggatgcg gacggctcgc aactttccgc cgcggccaag cggaaggcgc
      121 ggcgggtgca gtccagcgaa tctccgggga tcctctcatg ctttctgaga gcgagcagcg
      181 aacgcagggc aaggccctgc tcgaaaagag cgcggcagtc cgggcattcc tgcgcgtgag
      241 cgaggcattc cggagtgagc tgttcagcgt ccagcgtttc cttcctgaat ctcgagcatt
      301 tcatggcatg taatccttca gttcctgctg cagaagccgc cgggcataaa acagccgcga
      361 gcgcacggtg ccgggcgaac agcgcagcat tctggcgatt tcttcatgcg gtatcccttc
      421 catatcgtgc atgaccacca ccgttctgtg tttctctgac agcgtctgca gcgctttgtt
      481 caatttttcg tgcagctcgg agacggaggt ctcatagcgg gggccctgat gcgcctgaag
      541 ccggacaaag gcggggtctt tttcgatgga atcgtccatt tcgttgaggc tcagcgccgc
      601 acggcggttc cgttttttgc ggtagttgat ggtgtggttg acggcaatgc ggtatatcca
      661 ggtgtagaac gaggctgttc cccgaaagcg ccgcagggac cggtaggctt tgatgaacac
      721 ctcctgaagg atgtcctgcg catcctcgcg gttggaggtc atcccgtaca ccagcgcata
      781 gattttctca tgccagcgtc cgatcagctc ctcaaacgcc gccgcatcgc cgttctgcgc
      841 acgggccacc agctccgcat ccggcgggcc gggctcctgc gcggcattgt ctttttcagt
      901 ttcaaccatg ggaggcatta tgcataatat gcgaatgaac agtctaacaa aattcatgcg
      961 gatgggcatc gccgccgcgg cgatcgccgc cccgcgcggc gccgcggaag agcagccctc
     1021 tcccaattcc gtgttcgtgc agaacatgta cacccggcag atggcatccg ccgccggttc
     1081 cagcaatttc cttgtgcggc ccggaatctg ggccgaccgc accgcgaaga aggtcagctt
     1141 ctgggcggaa gccaccggca tcacgcccgg cgagccggtg gaattttatg tcatctcacc
     1201 caaaagcggc cacgcctatg aggcgctggc cgtgtctttc gcaatgccgg gcgatgtgca
     1261 tgcggcgctg gagttcatcg gcatgaaacc gggttccgtg gtggattatg aaagcctccg
     1321 catctggccg aaaggcgaac gagtgttcct gaccttccag tgggagcagg atgaaggcgt
     1381 gctggttcgc cggcccgccg aacagctgct ggtggatgcg gacaccggcg ccacgctgcc
     1441 ggaaaagggt ttctgcttca cgggttccgc cgtcatggac ggcgagtatg ccgccgacgc
     1501 cgaggagccc gggagcatcg cgtccgactt caacgaccgc gccaccgtgc tggatgtgcc
     1561 cttcaagagg gataaaagcg gagtataccg gagattctcc gccaatcccg acctgcagat
     1621 tccccgcggc aggctggtgc agattgacat tgaaccggaa tacatggacg gccggctgcg
     1681 catcgcggac atcacgcttt cggtttccgc tcctgaaggc gcggggtgga aagacctgat
     1741 ctgctccgcc ggcttatccg gcgcaccgac cagcaccacg cccgaagggc tcatggaata
     1801 cctccaggaa ctgatcgaaa acgggagaac gccccatgtg gatctgaggt tttcgccgga
     1861 tgtccagctg ggccatgcgg ttgagctgtg ccggtttctt gatctgctct gcacggacaa
     1921 cggcatccgc atggaaccgc cctcgaaggg acagcttttt tacaaggcgt ttctgccgca
     1981 gcccgaattc cgggcgcgtg aagaccggcc cagtcagccg tgggaaattc atgcaggcgg
     2041 cacgaacggg ctgaaactcg tgcagatcag cgaggagtgg ccggatgatc agccgaaacc
     2101 cgatctgaaa atcgcggagt tttccgcggc gaatgccgcc ggcgctctcg atatcatccg
     2161 cgaaaaaggc gcgggcattc ccgtggcggt gatatttgcc ccggagaccg cgccgtacgg
     2221 cgaactggtg agcctggccg gcgcgctgcg cgagccgttc ccgaccgtgt acatcttcag
     2281 cggaagcccg gaagactgac cgcagctcag ggaaccggga atttcgcggt cagctcgatg
     2341 actttcttct tgagcagggc cagcgccttg ggatgctcgg cctttcccag cgcctccgcg
     2401 atcagtccgg caatggcgga catttcgccc tccttcattc cgcgagtcgt cacggccgga
     2461 gtcccgatcc cgatgccgga agtcacgaat ggactcctcg catcaaacgg aatggcgttt
     2521 ttgttcacgg tgatggcggc cctgtccagc gtgtccgcgg cgtcctttcc cgtcatgttc
     2581 acgggcgtca gatccaccag catcaggtga ttgtccgtgc cgcccgacac aatgcgcacg
     2641 ccttgagcgc gcagcgcatc ggccagcgca tgggcattgc ggacaatctg ctttgcgtat
     2701 tccttgaatt ccggctgcag cgcctcgtgg aaacaaaccg ccttggccgc gatcacgtgc
     2761 atcagggggc cgccctgcac gccggggaat atctggcggt caatatcctg cgcgaagcgc
     2821 gactggcaca ggatcatgcc gccgcgcggt ccgcgcagtg tcttgtgcgt ggtggtggtc
     2881 acaaattccg cataggggac ggggctggga tggcatcccc cggccacgag gccggcgaag
     2941 tgcgccatgt ccaccatcag gtaggcgccg acgctgtcgg cgatccggcg catccgcttg
     3001 aaatctatga ttcgcgaata ggcgctggct ccggcgcaga tcagccgcgg cttgtgctcc
     3061 gcggccagac gctccacttc gtcgtagtca atcatttcgg tttcgcggtc cactccgtag
     3121 tgaacgcagt tgaagaaccg gcccgagaaa ttcacgctgt gcccgtgcgt cagatggccg
     3181 ccatgggcaa ggctcatggc caggatggtg tctccgggct tgagcaccga aaaatagacg
     3241 gccatgttgg cctggcttcc gctgtgcggc tgcacgttga catgctcggc gccgaaaagc
     3301 tccttcgccc ggtcctgggc cagccgctcg gcctcatcca cgaattcgca cccgtgatac
     3361 caccgcttgc cgggatagcc ttcggcatac ttgttggtca tgacggagcc ggtcgcctcg
     3421 cgcaccgcgc ggctgacata gttctccgag gcaatcagct ccaggttttc cttctgacgg
     3481 cgcgtctccg aaacaatcag cgagcagatt tcgggatcgg agtcatacag ggcgaccgga
     3541 tcggtcagcg tggccatgta gccggtgatc ttctggaccc ggcgctcgtg gcggccgcct
     3601 tcgaactcgg ccgcgaacca ggcatcgagg atcgcatcca tttcgtcaag aggcgtgacg
     3661 gaagcgccca tcgccagaat gttggcgttg ttgtgcttgc gggccatttc cgccagatgc
     3721 ggcgtgccgc agagcgcggc gcgcacccgc ggaaacttgt tggccgtgat gctgactccg
     3781 atgccggagg tgcagatgac aatgccctgc ttggcctcgc cgcaggaaac gcgccgggcg
     3841 acctcggccg cataatcggg atagtccacg gattcgcggc tccagcatcc gacatcaatg
     3901 accttgattt tccgggcttc caggtactgc ttgacatgtt ccttggcttc aaagcctccg
     3961 tggtcagagc caattgctat tttcattttc cggtcctttc aaaattccgc gccatgaaga
     4021 gtatcaggtc ggccactgca gagtccacct catccctcgt ttttcgatac acggttttcg
     4081 ccccgcccgc cgggtccatt acatcgcgga cgcagccctc cgttccgaat tcatgcatca
     4141 ggaagacctt gccgcgcgcg gcgggaaact gattcagcaa ttcccgccgg tggccttccg
     4201 tcatggccac aatcagggcg gcggattcca ccatctccgc cgtcacctgc cggctgcggt
     4261 gggggcggat atcaatatta agttcctgca tcacgtcaat ggcattttca aacgcggagg
     4321 cgccatccac ggcatagatg ccgcacgatt caaccgccca gcctgagttc tcgcccagcc
     4381 gcgcgcgcag caggaattcg gccatcgggc tgcggcaggt gttgccggtg cagacgaaca
     4441 gaatccgttt ctctgccggc ccggacacca gggccgcggc gatttccgct ccggggatgg
     4501 ccccttcgcg caggatgcgc ggctcctctc ccgtaacatc aaccaccgta ctgggattct
     4561 ggccgcgggc ggggccggca tccagcacca ggtccgcgtg gtcacccagc gcggccagcg
     4621 cttcggccgc cgttacggca tccttctgcc cgctcaaatt ggcgctggtc acggccagca
     4681 cgcatccgca ggcatccagc agcgccagcg gaacaggatg atccggcacc cgataaccgc
     4741 atgtgccgcc gttctgcggg agcaccagcg tcagcgggcc gggccagaaa cggtttgcaa
     4801 gccggcgggc cgcggcattc agccggccgc cggccgcttc cacatcttcg atgccgcgcg
     4861 ccaggcgcgc caccggcttg ccgctgtcgc gccccttggc ttcgtacagg cgattctccg
     4921 cccccggcac gggcggcatg gccgccacgc cgtacacggt ctcggtcgga agaatcacca
     4981 ggccgccggc gcgcagcaca cgcaccgctt cgtcaatcac acccgaagga atgtccgccg
     5041 gatcagttct atggatttgt ggagtcttca cgaaatttta ttttaagcac gcgcaggccc
     5101 tgcagcagat cgcaggcccg gcgaagcgca ggatcatttt tcagccgcgg gtgaatctct
     5161 tcctccgcct ggtctgtcgc tccgctgggg cggccttcaa caacgatatc aggcgtcaac
     5221 ccctcgttgg cgcggtactc ccggccgtcc ggaaatttca ggatgcggga ggccacaagc
     5281 atggcatggc ttccgttgag ttccagcacc tcgcgcagaa gaaagtcacc ggccgtgcgg
     5341 cggccaagaa tcatagttcc ggtttcggag ctctgcaaac ccgccgccag cagttcggca
     5401 ccgccggctg tggtttcgtc aaccagcacc atggtcggca gtgtgcgagg ggaagccccc
     5461 tcggacaggc agatttccgg atctccggac agaggcacca tgcggaaaag ctctctttca
     5521 gagggaaaga actggctgag tatccggcag gtttcttcac gcgatgcgcc ggcggccgac
     5581 cgcagatcca gcaccacgcc atagactccg gaagttttcc attcctgcag aattggtctc
     5641 agggcatctg ctgttcccgg atggcatccg tagagtttga tgtatcctgt ttcgggggaa
     5701 attttttcgg aggccgcaac cggcgggatg ggtgctgcga ttcgcggcag aacgatttca
     5761 tgctccgtgc tgccctcccg gcgcaccgtc aggcgggcct cttcactggt attacccgca
     5821 agcagccgcc agacctcctc ctccggaagc ccccggatgc cctgcccgtc caccagttca
     5881 aggacgtcgc cgggacggca cggttcagga gccggtgcgg caattgcgaa ggatccattg
     5941 gtcaccataa aatgaaggcc gggcatgccc cactgccccc tcgcttcctg ttcccggcaa
     6001 gaaaggtcct ccgggcccag cagaacgccc ccggggtcgg ccgcatgcac caggtttgag
     6061 agcatgccgg cctccagcct ttcaggcccg gcaataacac cccgctcatg gagcagatcc
     6121 atggcccgca ccagggtttg tactgccgtg gcaggagctt cttcgccgca cacagaagcg
     6181 gcggcagccg cgcacaggat tcccgccagg atggttcgct tcaagtcctt cattttttcc
     6241 cgccttcagg gtcaagcgcc aggtgaatgc ttttaatgca gccggcgccg aaacgggcct
     6301 tcagtttttc catgatctgc ttccggccat agcgtgacag ttcgctgagc catgcggaat
     6361 gatccacata aaccgtcagc acatcgtgat ccagtttccc gggccgggta tggtccgcga
     6421 gctgaggacc cacgagccgg ggccactctt cctgcatggc cgcttcgtgc agccggccgt
     6481 gcatcccgat tttcttcatc acgtcgggaa ccacttccgc cagcggatgc acttcaaacg
     6541 gcgggggcgg ggcgggcaca tccgtggcaa accgctcctt ctgcaaagcc catgcgcccc
     6601 tgtcaaaatg caactttcgc cctgtggtca taacatcaac ggaaagagtg agtcgtaagc
     6661 agccaaagag gttcaggttt gctttttggg ggtaatccca tggcggatca accagtccac
     6721 agtagcgtga tcccagccca catcacggcc ggcgcgctcg ctttcatacc atttatgcgc
     6781 aaggatatcc tgctttctgc gttcccaatc cttgacaagt cgcgtctgcc tgacccactg
     6841 ctctggatcg ccgtgaccgg aacctgaatc ttgtgcgctc atattaccaa cacctgactt
     6901 tgactgaaca tcaaatcata aacgcgcccc ccctgccgga tcaagcgttt aacttgaaat
     6961 tttttgtctt cagtctggac gatctgcggg ctttagtcta gtttgtgccc gcaaacgcag
     7021 gtgaaaggac atactcacag atgaagaaac gtacagttcg gtttttcgca ttcgccatca
     7081 tttcctcatg tgctttgctt ccggcccggg cccagcttta tgccgggcgg gtggtggatc
     7141 cgctggacca agatttcagc atcacactca agccgggact gatcacctcg gtcagcggtt
     7201 atgtcaagga aaccaagcgg gcctatacgg atattcccgg ctacacgcag gattacgggc
     7261 gatttcttca gaactacgat ctggaggagc tgggttttga cgagagttac ggcgcgctcg
     7321 gactcggcat cgaaaaacgc tggcgtttcg tcaccttcct tctagatgta tcctatttcg
     7381 aagcttccgc cacagccacc gccaaggatg agccattcgc catcggtgtt gaagaggtga
     7441 aatttgaagg aaagagctac gactatatgt tcatcccaca gggcagcagc gccgatgtgg
     7501 atctgcaggc cggttcagcc gaactcggcc tgcttctgac gcccttctgc atcagcactg
     7561 aggaaggctt cgatttctca ccatggcttc gggccggcct gctggccatg ggcggaagct
     7621 acaccattga cgcgggcccg gcgcaggggg tccgaacgta cgaataccac ccttatccgt
     7681 atgtcatcgg cggcaccgga gaaggctggg ccggcatcgt cattcctgaa ttcggcattg
     7741 gcggcgaaat gcgcatctgg ctcaatcccg aaggaaccaa aattgtatgc cagggcaact
     7801 acatgatgct cgaatgggcc ggccggagca gcagcctcgg cgcggattcc cgcaacgaca
     7861 aggacattga tctctcctac aaatccattg acctgaccgt gatgcttgag cacccgctgt
     7921 cagaaggggt tgatctgctt ctcggggcca ccttccggca cctggaaacc gacgccacga
     7981 tcgaggcccg gcataaatcc gccgaggagc aggacgctgc gcgcgagaaa tatgacaagg
     8041 aagccagcat tgaaatgaac accttctacc ttctggccgg gcttcggttc tagattgcgc
     8101 ctcctgtcag gaaggcccga attcgaaact gttttgcgtt gcccgccttc acggaataca
     8161 gtacaaagcg ggcgttgcta tggagtggag taaccgtaaa cataaggaga aaatcatgga
     8221 aagattccac cggatagtcc ccgttctggc cgccgccctg gccctgtgtg cacattgcgc
     8281 attgggctcc ggctttgcaa tcgtcgaaca aagcgccagc ggcatcggct ccgccttttc
     8341 cggcgcgggc acttccatcg gcgacgccag caccattaat tcaatccggc cggcatgagc
     8401 cgcgtgcccg gaagcgaact gacacttggc gggcacatga tcatgcccac gttcgagttc
     8461 gacaacagcg gatcggtcat cctgcccgcg ggcgttcctg tttccggcgg tgacggaggc
     8521 aacggcggcg tggccgcgtt tgttcccaac gcctattaca aggccgatct ctgcgagaaa
     8581 accgcatggg gtatcggcat cacagctccc tacggactga ccaccgagta caacaaatcc
     8641 tgggtcggcc gctaccacgc catcaagact gaactggtga acatgaacgt caaccccagt
     8701 gtgtcttata aaatatcgga caagattgcc gtcggcgcag gggtcagcgc ttcgtatgtt
     8761 gaagccgacc tgaccagcgc tattgatttc ggcacgattc ttggagtggc gccccaggcg
     8821 ctggacggac gagcaagggt ggagggcgat gactgggccg ccggatacaa cgcgggggtg
     8881 ctgatcactc cgcgtcccga atgcgcgctg agcctgagct atcgctcaaa gattgagcat
     8941 aaactcgaag gcgatgcgtc cttccaggtc cccgggcccg cccgggcgct gcaggccatg
     9001 ggcatgtttg tcgacaccga cggcgaagct gaattgaccc tgcctcagtc cgcgtcgctg
     9061 ggcatggccc acacctttgc agaaaaggtc accgtaatgg ccgacctcac atggaccggc
     9121 tggggcgatt tcgacgaact gcgcgtcaag tacggctcca gccagccgga cagcgtgacg
     9181 gacgagagct gggacgacac ctggcgctac tcgctgggcc tgcagtacca gcagacggac
     9241 aagctggccc tgcgcatcgg cggcgcctat gacgaaaccc ccgtgcccga tgccaatcac
     9301 cgcacgccgc gcattccgga tgccgaccgc atctggctgt cgctcggcgc cggctatcag
     9361 gtcagtgaaa acctgagcgt tgatgtgggc tatacgcacc tgttcatcaa tgattcccgc
     9421 gtccagcaga tgggcgccac aggcgactat ctgaacggct cctatgaggg atcggtggac
     9481 attgccagca ttcaggcaaa ctggaaattc tagccgggcg atgaactgtt ccgtgtgaaa
     9541 cgggagcggg cagcgaagct gtccgctccc ttctttttcg gagttcgcca tgaaatacat
     9601 tttagcactg gatcagggaa caaccagctc acgggccatc ctgttcaacc gccaaaccga
     9661 gggcctttcg acagggcagc aggagtttga ccagattttc cctcagtcgg gctgggtcga
     9721 gcaccggccg gaggacatct ggaattccca gaaggatgca gcggttcagg cgctgaaaac
     9781 gggccgggtg aaaaccgacg acatcaccgc cgtgggaatc accaatcagc gggaaaccac
     9841 gctcgtatgg gaccgcaaaa ccgggcggcc agtatgcaac gccatcgtgt ggcaggatcg
     9901 ccgcacggca gagctgtgcg agaggctgaa ggctgacggc gccgaggacc tgttccgcgg
     9961 caaatgcggc ctcccgctgg acccctactt ttccgggaca aaaatccaat ggattctcaa
    10021 tcatgtcccg gatgccgccg cccgggcggc cagggggcag ctggcctttg gcacagtgga
    10081 cacctggctg ctgtggaagc tgacggaggg cagaattcat gccaccgacg cttccaacgc
    10141 cgcgcgcacg ctcctcttca acattcactc gggaacgtgg gatgaggaac ttctgaacct
    10201 gctgggcgtg ccggcatcca tgcttccaga ggtgcgggat tccagcggca tgttcgggga
    10261 aattaccggc atgcccgaat tgaagggggt gcccgtgacc ggcatggccg gcgatcagca
    10321 ggccgccctg ttcggccagt gctgttttca gccgggaatg gccaaaaaca cctacggcac
    10381 cggatgcttc ctgctgatgt ttaccggaca ggacgcgatt tcctcccgga gcagactgct
    10441 gaccactgtc gcctggcaaa aggcaggaaa actgcactac gcgcttgagg gaagcatctt
    10501 catcgcgggc gcggccgtgc agtggctgcg cgatcagctc ggattgatta aaacggcagc
    10561 ggacatagaa ccactggccg cccgggtccc ggacagcggg ggaatattct ttgtcccggc
    10621 cttcacggga ctgggtgcac cgcactggga cccctatgca agaggaacca tcgtcggcct
    10681 gtcccgcggc acatcggccg cccacctcgc ccgggccaca ctggaggcga tcgcattcca
    10741 gacactggaa gtggttcggg ccatgcaggc cgatgcacaa acaggactgc gcgaaatgcg
    10801 cgctgacggc ggcgccagcc gcaacaatct gctgatgcag attcaggcgg atcttctgca
    10861 gcagagggtt gtgcgtccgg tcatgagcga gaccacagcc ctcggggcag ccgcgctggc
    10921 gggcattgct tccggtttct ggacatcgga aaaggatgtc gaaaaacact ggcgggccga
    10981 tcgcgtcttc gccccggctg cggcggataa acttgtacac gaaaagttcc tgcagtggga
    11041 ggaagccgtc cgccgggcgg gcggatggat taaacattga ccgcatggaa tgcgtcatta
    11101 gactgtctgg cggagaaaaa ggagcaattt atgaatgggg ctgcatttgt cgcgggcatg
    11161 tttattctgc tgggcacagt gattctgctg gccagcctga tccgcaccca gctgctgttt
    11221 gaagcgattc cgctcattct gtccaagagc cgggcgccgg tggtccgcat gctccggctg
    11281 taccggcacc tgctgctgtt catcatcctg ggttatctgg tcgtgattgc cggactggcc
    11341 ggtgaactct ggaatctgag cacagcagtt tccggtgccg tttttttctt cttttccgta
    11401 tatgccctgc tgtgcagccg gctccagtcc acgctgttca aggagctgga aaaaactctt
    11461 cacgggcttc tgcccatatg cgcggcctgc aagaaaatcc gcacggaggc acacaaggat
    11521 gagcccgtgt gggtgcctat agaagtgtat atcagtgaac gcacggacgc gcagttcacc
    11581 catggctact gcccggaatg cgcggaaagc atgctgtccg aattccgcag cgggaaaaag
    11641 tcgaaaaaat ctactcccgc cactgagtga tgcggtccca tgcaaggtcg cgcagccgct
    11701 tcaaaacagc cggatatttt tcctcgatat cagacagata ccattccagc agatcaacgt
    11761 ctccgcaggc ctccacccga aaagtgcgtt ttttctgggg gctgaccgga ccaatctcgc
    11821 caatgatgct gcccatgccg acggtttcca gcacggcgcc gtcctgaccg agaatattca
    11881 cacatccgtc cgcaatcagc gcgaaactgc ttccagactt ctgatccttc tcatacacgg
    11941 cagttcccgc cccgcagtgc cgcgttgtcg cccgcatcag cagccccccc tgctcactct
    12001 cgctgagttc ggaaaataaa tcacacgcca tcattgcgct aaccgcagcg tcaaattcca
    12061 tttttcacct cgtttccact cacgcttttt tcgtgtactc ctcacactga tccggcaata
    12121 taaccgctca gatgcagtga ggcaatgaat tgaatatgaa ctttcttgat ctcgcaaacc
    12181 ggcgtcacag cctgaggaaa tacgcagcca ccccggtccc gcgggccatc ctcgaccgct
    12241 gcctggaagc agcccggctg gcgccctcgg cctgcaactc gcagccgtgg cacttcattg
    12301 ttgccgatcg cgagcccctg cggacacagc ttgcggcggc ggcgttttca gggctttatt
    12361 ccatgaacgc ttttgcaaag tcggcaccgg tgctgattgt cgccatccga acttcttctc
    12421 gcgcatccgc cgcgatcggg ggcgccgtgc ggggaatcca gtatggactg attgatgtgg
    12481 ccatcgcctg cgagcatatc gtgctgcagg cggcagaaga cggagtcggg agctgctggc
    12541 tgggctggtt caatgagaag gccgtcagga aactgctggg catttcccgg acaaaccggg
    12601 tggacaccat catcagcctt ggatatccgg ccgaggaaac cggccggcca aaaatccgca
    12661 gggaactgga tgccatccgc acatttaatc ccgagccctg accggcttgt cagctatgga
    12721 gaaatggccg tggtgttctg ctgttcagct tccgagatcg gcggcaaaga ccggatatcg
    12781 gctttgagct gttccacggg cctgtgcagc agatcttccc gcgcggcagg atcgtttcga
    12841 acagctttaa cggtccatga tacctcgcaa tcctgatccg caccaataac aaaacggttt
    12901 ccagccgcct tctgcttcac atagcaaaca gcagcaacac ccaccggcgt gaaactgtag
    12961 acttcagatc ccgccagatt cagtgccgaa taatacgccg gcagctcgac aactgctgtt
    13021 cctccgatca cctgcgcgtt tccggcatat acattccaca ccttcggacc ttcgaccgaa
    13081 taatgtcgaa gcaccctgtt ttcgggatcc agcgggtgat caatggtgaa cgccttgaca
    13141 ctccaggctc ccgatccaaa ggtggaccgg tacatcaaac ctgttccccc gtctaggtaa
    13201 agcgagcccc tgatttgcgc actgtcatct gtgtcgttta ccacaccgga gcccatcgca
    13261 atccgattcc ggccggccca gcatttggcc gcatatccaa ttgagatatt ggtctgcacg
    13321 gcataactgt cgcggccaat ggccaccgca tactcacggc catgagaaaa atggccgatg
    13381 gaaattccac tgtttttggc gtttgctcca cgccccattg cggcgcccca gcttgatcca
    13441 tccgcttcaa acccaatggc cgccccgctc tggaagctgt ttgccgcctg gcccacggag
    13501 gccccggaag aagatccgtt tgccgtacgg ccaagggctg tccccacata agctccaaag
    13561 gccatatacc ccagggccgt cccgttcacc gctgcatttg ctctgtctcc cacagccaga
    13621 ccgcccccgg cgacattggc tgtattgcca atagcaatat tagcccccga actacctata
    13681 acaatcccgg ttactgagct cacattgatg acaagggctc ccgtcatctc atcgccaatc
    13741 tttgaaaccc ttgccgtttc tatattcagt attctgattt cgacatcatt gctggaagac
    13801 gcccaggagg gatcggtttc tgtgtatgtg taaagcggcg tgccggtggc ataaccggcc
    13861 ttctccgcaa tccagacggg atcagcttcc gcgtataccg gcatcccggt cgcataggcg
    13921 gccttctctg ccgtccagac ggggtcggtt tctgcgaaaa cataaagagg cgtacccgtg
    13981 gcataggctt cgcgttcggc tgtccagacg ggatcagctt ccgcatatac gggcgaaccc
    14041 gtggcatagg cccccttctc cgaaagccat acagggtctt cttcgaccat tagctcctgc
    14101 caagggcccg ttcccgccgt gctccgataa tagatattgg tgccgccgtc aagatacagc
    14161 gtcccgcgaa cagccgccgt gtgatccacc tcgttggtca cattgtgtcc aatggctatt
    14221 cgctcttctc cgccctccgc actggccgag ttcccaatgg caatgccgga cccctcgctc
    14281 agaataagaa tgccttcacc cgccccggca ttgatggtca gcggaccggt catcgtgtct
    14341 ccggccttgc ttaccgccgt cagggcatgt gccaccgccg cgatctcctc gcgcggagaa
    14401 agaaccgtgc ccccgatttc cacctcaagg aaacagctgc ttcccttcag ggccgcgagc
    14461 aatgagcccg ccaccgtatt tgacaggcca atggccgtgg aatagaggcc atccaccacg
    14521 ctcaccgtct gcgtctcgcc gtacagctcg atcccggcgg acacctcgtc gaaaaggcgg
    14581 aaaataatct gacgtgaccc gttgacgaga ttggtcccct cgatcagccg gccctgataa
    14641 ttgactcgtt cgggcggctg cagggcaaaa cccatcgtcg ctgcgagata cagcactgtc
    14701 gaaacgagag cgtttttcag cttggcggta ataataacct cgctttctta aaacatgaat
    14761 tcctgccaga ttaaactaca cggggccaca agatctgttc aatgcccgcc gtgcgcagaa
    14821 gtctttgtat cgccgcacgg ggatcgggag actttgtcac tgcgcgaacc accgcgaagt
    14881 tttgcgcgcc gtgcgcaagc acatcgggca gattctccgg cgtgatgccg ccaatggcga
    14941 ccgcaggcag actgaccgcc tgcagaattt ttcgcaagcc ctccagcccc accgcgggat
    15001 ccggaatctc ctttgttggc gtcgggaaaa cggggcccac gccgatgtag tccggattct
    15061 gcatgacggc ggcacaggcc tggccgagat tgtgggttga aaggccgaat atcttcccct
    15121 ccgcaggcca gcgcgcacgc gcctcctgca gcggcatgtc gtcctgaccc agatgcacgc
    15181 cgtcggcatc cgccgcccgc gcgatatcca cgcggtcatt gacgataaag agtgtttctg
    15241 agttctgcgt gatcgcacgg atttttacgg ccatgtcaag caaagcaaca tcggatgctt
    15301 ttttcatccg gagctgcagc atccgcaccc cgcagtccac ggcggcacgc gcgcattcct
    15361 cgtatccggc cagcggatcc gtcatcacca gatagagtcc aaacggcttc atgttaccct
    15421 gtatacaccg catcccagtt cttgaaaacc ggctcgattt tttttgcctg gagcatatcg
    15481 cagatttcca tcacgctgcg cctgtcactg acctcaaact gcccgcgatc ttccgcgggc
    15541 gcgccggcgt atccgcccac cgtggtgcgg cttgcggcac tcatgcggga aatgccgaca
    15601 cccgccattc cgtcacggaa cgatggcttt tcgcgcgtag aaagcaccag aggcacatcc
    15661 ggcatcacga tacgaaatgc aaagacaatc tgagccagca ggcggtcctc caccggatgc
    15721 gcaggctgga atccgccctc ctgcggccgg atgcggggaa atgaaatcgc aatgcccgaa
    15781 cgccagtatt tttttcgaaa ttcctccgcg tgcagataaa gggctattga atccacgacc
    15841 ggatcagaca atcccagcag aacgcccatc cccacagttc tcatccccgc ctccagcacg
    15901 tgcgcgggag cggacacccg gccggcgaaa tccttctttg gcccccaccg gtgcatcata
    15961 tcatagacga caggatgata ggtttcctgg tagagggtca cccccacgca gccggcgagg
    16021 ttcaacttcc ggtattcatc caccgtcatg ggaaacgcct caacctgaac ctgggggaaa
    16081 agttccgacg ccatccgtac acatgcctgc acatattcaa agtccgcctg cggcgtgcgc
    16141 tctccggtca gcagcagaat ttcctcaaaa ccctcccgct tcaaggcatc catctcgcgc
    16201 gcgactgcat caaacgggag tttccgccgc ggctgcttgc ggtcggaagc atatccgcag
    16261 tagacacacc cgctggagca gtagttagac agataaagag gtgcatagag cgacaccgtg
    16321 ttcccgaaat gccgggctgt cagactcttc gcgcgcgcgg ccatctgctc gaggtgagcc
    16381 cgcgcctgag gctgaagcat aagcgccagt tccctcggcc cgggccgctg cgcgcccatc
    16441 gcagaaaaga tctgccgatt atcggcccgg ctcgcggccg tattgattgc ctgcgccaga
    16501 ctccagtatt gtgacgggtt atccatcagc cgagaaataa agtcaggggg ctggttgcag
    16561 ccgccgttgt actgcgcgcc atcaggccgg cttcatatgc tgcggaaccc gcctgaacgg
    16621 cggcagcgaa agcctgcgcc atcgcggacg gatttcccgc tgcggcgatc gcgctgttga
    16681 caagcacagc cgcgcagccg gcctccattg ctgcggccgc ttcagacggg gcacgaagtc
    16741 cggcatccac aatcacagga acattgctgt tgttaatgat gatatcgagc agcccgccgg
    16801 cggcgatgcc gttgccgctt ccaatggtcg aacccagcgg catcaccgag gcgcagcccg
    16861 cctcctccag ccgcttggca agcaccggat cggggggcat gtaaggcatg acaataaaac
    16921 cgtcggccgc aagaatcttt gcggcctcat aggtttcaat gggatcgggg agcagatgat
    16981 gcggattggg atgaatctcc accttgatca gccggctccc gcagagttcc cgggcaaggc
    17041 gggccgcctt cacggcttca gcggcatccc gcgcgccgga agtgttgggc ataagcgtga
    17101 tgttcttcag cgtcatcagc ggcccgagca ggtcatcgga cggccgctcg cggttgaacc
    17161 ggcgcagtgc cacggttaca atctgcgcgc cggatgcctt cacggcctcc agcatcaccg
    17221 cggagctgga aaattttccc gtccccagca gcaggcggga ctggaattca agatcgccaa
    17281 gtacaagcgg cttcatgatc atccccctgc ggcgaatgtg agaatttcaa tccggtcgcc
    17341 cgcccgaagg gtaacggcct ggcgttcagc ggcggaaatc accgtcccgt tcaccatcac
    17401 ggccacgcgt ttcgggcttg cgcccatctg caccagcaga tcagggagcg atgatccgga
    17461 ggactcgatt tcttcgccgt tcacaacaag tttcatcatc tgctctttca aacttggata
    17521 cgccggaggg aacaaaggcg gccatctccc ttcgccggca tgatccggat caggtaccgc
    17581 gggtatgatc tcagccccct ttcggggaca ccccggcaac cgtttagaaa gataaggggt
    17641 tggcggatca aagtcaaacc cggcgcgaag gcataaatat ctgcttgccc gcaaagcgga
    17701 catgatagaa tctgatgaaa acacatcagg gatggcggtc atgaacagtg gcgataaaat
    17761 cgagcaggcg gcaataccga tgcggcatat cgggccgtta aaaatgtccg gacccgcggt
    17821 cgagggagag ttcatgcttc cgctggcgac gtatgaaagc cccctgtggc cttcagtcgg
    17881 ccgcggagcg cgcgtttcca tgcaaacccc aggactatgt gcggtcatca cggatgaccg
    17941 catgagccgt tcggtgctgg ttcaggcagc caccgcagtg gaagcatgga agattgcctc
    18001 cgccctgcag ggccggcacg ccgaaatcgc cgcggcggcg gagtcttcca gccgattcgc
    18061 cagattcctg gatctgcatc atcaggtggt cggcaacctt ctttatctgc gtctggaaat
    18121 gcaaaccggc gacgccgccg gacacaacat ggtgaccaat gccgccgagg cgttcctcaa
    18181 ctggattctc cagcagtatc cgcagctgaa gtacgtatcc atctcggcca acttctgcac
    18241 ggacaagaag gcgacagccg tgaacgggat tctgggccgg ggacgagggg tcatctgcga
    18301 gacggtgatt ccccgcgaag tgtgcgcgaa ggtgcttcgg accaccccgg aagccgtggc
    18361 cgacctgaac ctcaagaaaa acctgatcgg cacctcgctg gcggggggca tccgcagcgc
    18421 caatgcgcat ttcgccaaca tgctgctggc attctacctg gccaccggac aggatgcggc
    18481 caacatcatc gagggctcgc aggggtatac tctggccgaa gtccagaaca atgacctcta
    18541 tttctcggtc acgataccga acataatcat cggcaccgtg ggcaacggca aggatatcga
    18601 gtttgttcgc aagaatctgg aagcgcttgg ctgcctcgaa aaacggccgg ccggggagaa
    18661 cgcccggcgg ctcgcggcgc tggccgccgc agcggtctgg tgcggagaac tgtcattgat
    18721 ggccgcgctg acccatccgg gggaactgat gtcagcgcac cgaaaaatgg aacgcaggaa
    18781 atgaacgagt ccatcaacca gcgcaagctg gaacacctta aaatggcggc cgatgaatcc
    18841 gcggaccgcc agaaaagata ctgggatgcc atccatctga cgcaccgcgc gctgccggaa
    18901 atcaacgcgg cggatgtgga tgcctctgca gagttccttg gaaagaatct gacattcccc
    18961 ctgctcattt caagcatgac cggcggggac gatcagcaga tcctgcggat caaccgccat
    19021 ctggcggccg cagccgaaac cacgggggtg gcgctggccg tcggatcaca gcgcgtgatg
    19081 ttcgacaatc ccgaagccgt ggagagtttc cgcctgcgcg ccctcgctcc cagcgtgccc
    19141 cttctgtcca atctgggggc ggtgcagctc aatcacggtt ttacggaaga acattgccag
    19201 aaggcaatgg atgtgcttga ggcagacggg atatatcttc acctgaacgc cctgcaggaa
    19261 accgttcaga acggcggcga caccaacttt gccgggctgg ccgaaaaaat cgggcatgtg
    19321 gccggaaagc tgaacaagcc cgtgctgatc aaggaagtgg gcgcgggcct ggggccggcg
    19381 gatgtggaaa tcctgatgaa acaaggcata cactattttg atgtggcggg caccggcgga
    19441 acttcatgga gccgcatcga gcagttccgg cgggtctgcg aagacgcccc cgacctcggc
    19501 tttctttatc aggactgggg ctggccgacg cccctcgcgc tcaggaagct tgctccgctg
    19561 cgcgaccgga tcacgctgat cgcctccgga ggcatccgca ccggcgtgga tatggccaaa
    19621 gccgtcatcc tcggggcctc cctgtgcgga atcgcccgtc cttttcttaa gccggccatg
    19681 gtttcggcag aggaagtcgt caaggtcatt cgccggctca agaaggagtt catcgccgcc
    19741 atgttcctga cgggcatgaa ttcggtttca aagatgcggg gtaatttatc gctcgtccgt
    19801 acagatgatc cggaataaat gaaagcccgc gcaccaggca aactgattct ctccggtgaa
    19861 catgcggtgg tgtacggccg gccggcgctt gtaaccgccg tcaaccgtta tgcggattgc
    19921 accatccact cagaggaccg cgatgttttc cggtttctgc tgaccggact cgataaagag
    19981 ctttcgcttt cagcaaaaag tctggccggg ctgcacagga ctttgaacga aagatatgaa
    20041 agattcagac tcggagagct gccgatcagg gacgtccacg ggcggccggc ggaactgacg
    20101 gcctatgcgc tggcctgttt gctgcagcac accggcatca caccggcggg cggattaacc
    20161 tttgaactgg aatcctccat accgcttgga tcgggcatgg ggtcatcggc agccattgta
    20221 ctggctgtac ttcaggcggc ggattcgctg ctgcgcggcg gtcttgagcg caatgtgtta
    20281 tttgatctgg ccatgatgag cgaaagactt cagcacggga atcccagcgg agccgacccc
    20341 tatgtctgcc tccatggcgg gctgcaccgg ttccagaaag agcacagtgt cagcctgccc
    20401 tgccccggaa ctgcttatcg cctggcacag accggacggc cgcaggcggg cacgggcgaa
    20461 tgcgtatcct ttgttgcaag gcattttgga accagcggga tctggcgcga atttgaactg
    20521 ctggaaggcc ggatcgaaaa agccctgctt tctggcgaat ggggcattct tcaggaggtc
    20581 gtgacggaca acgaggccct gcttgaaaga atcggcgttg ttccaaaacg ggttgcggcc
    20641 ttcatccgcg acctgaaccg gcagggatgc gccgccaagg tcagcggcgc cggatccgtg
    20701 tccggggatg atcatgccgg cattgtgtgg atagtttctg aaactgcccc gacatctctg
    20761 tgcgatgaat acggatattc actgatagaa accgaggggg atgccgatgg cacgcgcatc
    20821 gtttagggtg tccgctcccg gaagcctgat gctgctgggc gagcatgccg tacttcacgg
    20881 caggcgctcg ctatgcgggg cggtggacaa gcggatggaa gtgaaactga cgccgcagaa
    20941 atctggaacc cttcatatta tttccgcgct cggttcatat gagtcgtccc tgcgggatct
    21001 ggcgccgtcc cgccgttttt cctttattat tcacgctgtg ggaaaacaga gggacagact
    21061 gccgggcgga ctgcggctgg atgtgaaatc gggattttcc agcacggtgg gactggggtc
    21121 atccgcggcc gtcacggtcg cgacaacggc agccctgatg gaatgggccg ggcagtcgta
    21181 cagccgcgaa gatattttcc gcgaggcccg cggcaccatc cgcgcggtgc aggggctcgg
    21241 gtccggtgct gatgcggccg ccagcgtctt cggcggagtg ctggtctacc gcatggatcc
    21301 gctggaaatc cgcaggctga cctgctgtcc gcccctcacg ctagtctatt ccggatacaa
    21361 ggaaccaacg gtgaaagtgg ttcaactcct ggaacagcgg cgcaataatg atcctgaata
    21421 ttttgaacgg ctgtttgatg ccattgacca gagtgtgccg caggccgccg gggccgtgga
    21481 agcgcaggac tggccggcgc tgggaaaaat actgaacggc aatcaggaat ttatgtcgca
    21541 aatggaactc agcacacctg atcttgaccg aatcattgaa atcctgcgca gccgcccgaa
    21601 cattttcggg gcgaaaatat ccgggtccgg gctgggcgac tgcgccgtgg gcataggcga
    21661 gatccggctg cgaggtattg aatatgagca gattgatgtg aaactgtcac cgcggggggt
    21721 ggaaattgac tgacaaagaa gccgcacaac tgattaccgg cgcggcaaaa gggtgtccga
    21781 aggaatgcgg acaggcctat gccccggtga acatcgcgct ctgcaaatac tgggggaagc
    21841 gcgacgagat gctcaatctt ccggtcacct ccagcctgtc gctctctctc ccggaactgg
    21901 gagccaccac ccgaatcagt tttcgcgaag gacacgacca gctttttctg gacggagtgg
    21961 aacagtcccg ggattcagtg tcggcacggc gtgtatcgaa gtatctggat ctgttccggc
    22021 cggaccgcag caccttcttc catgttgaaa gcgaatccac tgttccgatc gcggcggggc
    22081 tcgcctcatc agccagcggt tttgcggcgc tggcgctggc actgaatgat ctcttccaat
    22141 ggaatctcgg tctgcgagac ctgtcgattc tggcgcgact gggcagcggg agcgccgcgc
    22201 ggtcgatata caccggattt gtgctgtggc atgcgggcac gcgggaggac ggcatggaca
    22261 gcttcgccga gccggtggac ggggaatggc cgggcctctg catggggctg atcatggtct
    22321 cgcgccgcga gaaagcggtg tcgtcacgca tcgcgatgaa gcgcacagtg gacacctccg
    22381 cgctctaccg cgcatggccc gcgaaggtgg agtcggattt gaagaacctg cggcaggcca
    22441 tcgaaaaaaa ggatttcgac ctgctggggc ggaccgcgga atccaatgcg ctggcgatgc
    22501 acgccaccat gatggattca cagccgccgg tactctactg gatgccggaa acggttgagg
    22561 aaatccggaa aatctggcgc ttgcgcgaca atggactgcc ggtgtatttc acaatggatg
    22621 ccggccccaa catcaaaatg ctcttcatgg aaaaggacgc gtccctgatc agggaacatt
    22681 tcccggacat ggaaattgcg ctgagctgat tcccgccgcc tcccgcgttc aaattacttc
    22741 tggttttccg gctgatcagc gagtaaaatc actgatgttt aaaggcgtcg ccttgtttaa
    22801 attaagttgg attagaggcc catggcggaa cacaatgcga acaaaacggg ggatttgccg
    22861 gaattgcccg ggcagaaaag cagtgcctac cgcgatctct tcgagcacat ggcggcggct
    22921 tcctgtctgg acgaactgat ttacagggat ggaaaggcgg tggattaccg cattctggag
    22981 gtcaatgcct ccttcgaacg catcaccggg atcagccggg cccgggccgc cggttcattg
    23041 gcttctgaat tgtacggcat caaggacgtc ccctatctgg acattttttc ccgggttgcc
    23101 aaaacagggg agcccgtgcg catcaacgac ttttttcccc agtttgggaa atatctggac
    23161 atgatcattt cccggccccg cgaaggctgc ttctcgactg ttttcacgga tatcacggaa
    23221 cagcgcaagg cgcaggaggc gctgcgaatc agcgaaagca agctctctgc tgcgctgaaa
    23281 atggcccggg ccggtcattg ggaatacgat gtcgaaagcg acacgttcac cttcaacgac
    23341 aacttctacc ggatattcca caccaccgcg gagcaggtcg gaggctatca aatgtcctcc
    23401 gcgaactatg cgcgaagatt ctgccatcct gatgatgccg ccatggtggg agaagaagtg
    23461 cgcaaggcca ttgaatcgga cgatcccggc tattatcgcc agattgaaca ccggattctc
    23521 tttgcagacg gaggcgtcgg ttatatgtcg gtgtgcttct tcattgtgaa ggacagaacc
    23581 ggacgcacta tccgaaccta cggcgtcaac caggacatca ccgaccgcaa acggacggag
    23641 gaggaactgc gcgaaagcga ggaactttac cgcgcattca tcaattccac agaagacctt
    23701 gcattcatca aggatgacgc cttccgctac caggtggtga acagggcctg tgcgcgtttc
    23761 ttccagcgga ccgcaaatga aattttcggc agcaccgacg cagagctgat gacgcccgag
    23821 gcggcggcgc ggtgccgtat gacagaccgc caggcgctcg aaaccgggca ggtggttgtc
    23881 agcgaagaac aggtgggcga ccaggtcttt gaaacccgca agttcccggt gcggctccgg
    23941 ggcggacgcg ttggagtcgg gggggtgatt cgcaacatca ctgaacgcaa aatcacagaa
    24001 agaactctga aaagcgagcg ggacttttcc acaacggtaa tcgaaacact tcccggcatt
    24061 ttttactgct gcgacgagaa cctgcaaatt ctccgctgga accgcaactt cgaacgggtg
    24121 accggatata cggccgagga gatttcccgt ctgagcgcgc ttgatctctt taccggcacc
    24181 gagcgcaatc ttgtcgcgct gcgcatacgc gaagtttttg aaaaaggaga atcggaagtc
    24241 actgcggatc tttccgccaa agacggcacg cgcataccct actatttcac cgggcgggtt
    24301 gcggagctgg acggccggcg atgccttgcg ggagtcggca tggatgaaac cgcagtgcgc
    24361 caggcaagga aggcggggga ggaaagcaaa gcgcgctatg atctgctgtt tgaaaacagc
    24421 cttgatgcca tgctctttac ggcccccgac ggccggatat tctccgccaa ccgggccgcc
    24481 tgccggatgt tcggcatgag cgagcaggaa attgtccgcg ccggacgtga aagtcttgtg
    24541 gactcttcag atccggaact gggccggctg ctgaaagaac gggagacaac tggaagcgcc
    24601 tccggagtga tcaccatggt ccggaaggac ggatccaagt tcccggccga aatatcatcc
    24661 agtatcttcc gcgaagaaaa cggcgaactg cggaccagca tcgtcattcg agacatctcc
    24721 gcgcgggacc gggaggacag gaatatccac agccagctgg atgaactccg ccggtggagc
    24781 gaagtgacca tgggccggga aatgcgcata ctggaactga agcgggaagt gaatgagctg
    24841 ctgtcgaggg cagggcttcc gccccgctac tcaagtgccg gggaacaaaa taccggcgct
    24901 tcatctgcgc gggagccggg cgcatgaaaa aaagcgcgcg catctgcaca tgcgggtttg
    24961 ccctggcctg gatacttctt gccggggccc aagtgatctg tgccgccgag ggaacttcga
    25021 ccatcacctc cggatgcgag gtaggctatc ccccgttctg catcgtccac gagacgggag
    25081 aagtggacgg gttttcggtt gaactcctgc gcgccgcact gaaatccatg acccgtgatg
    25141 caacctttca gacaggaaca tgggacgagg tccgcaatct cctgatcgac gggaaaattc
    25201 aggtcctgcc gctggtcggc cggacgccgg aacgcgagga gttttttgat ttcacggttc
    25261 cctatctcac catgcacggc gccatcgtcg tgcgcatgga tcagacggga atcaatgatc
    25321 tggaggacct gaaagggaaa aatgtcgccg tcatgagcgg cgacaacgcg gaggaattcc
    25381 tgcggcggga agaccgcggg ataaatatcc gcacaacggc gactttcact gacgcgctgc
    25441 gcgaactttc ggagggccgc catgatgcgg tggtcattca gcggctggtg gcgatgcgcc
    25501 tcattcggga ggcgggcctt aaaaacctgc gcattgtgaa taaaccgatc accggattcc
    25561 gtcaggactt ctgtttcgcc gttcaggaag gcgatcgcga gacccttgcc ctgctgaacg
    25621 agggtctggc gctggtggtg gcggacggga cttatcggca tcttcacgcc aaatggttcg
    25681 ccgcgctcga aatgcctgaa aagcgaagca tcattatcgg cggagatcat cactatccac
    25741 cctttgaata tctcgatgaa aaaggccagc ctgcgggcta caacgtggat ttgatgcggg
    25801 ccatcgccct cgccatgggg ctgaccatag aattccggct ggcggactgg gtggaaacca
    25861 tcgaggcgct gcgcgacggc gacattgacc tcattcaggg catgttctat accccggcgc
    25921 gcgaccgcga tttcgatttt tccaccccgc acgccgtcaa ccagtacatc agcgcagtgc
    25981 acagatcggg cgggccgccg cccagaacta tcggcgaact gctgacacaa aacatcgcgg
    26041 tccagaccgg cgatgccatc catgatttcg ccgcagaaaa cgggctgacc aacctgacgc
    26101 tggtcgacag tcaggaacag gccctgcgca tggttgtgga gcaggaagtt gactgtgcgc
    26161 tggtgtcgcg cctttctgcg ctgcactgga tccagaagca cgggtgggat gagctgcggt
    26221 tgagtcgcga ggctctgatt tcactggaat actgctttgc ggtgtccgaa gggaaccggg
    26281 cgctgctggc cctcttcagc gaagggctgc gaacagtcga ggacagcggc gaataccgcc
    26341 gcatctatgc taaatggttc ggagttgacg agacgaaacg tttggactgg cgtatctata
    26401 tccgcttcgc ggccctgatt cttctgccgc tgatcctgct gctgtcagct gtttccctct
    26461 ggtcgtggtc gctcaggagg accgtggcaa ggcggacgga agaacttcgg caggaagtgg
    26521 ccgagcggca gcgggccggg gaaaagatcg aaatgctgct cacagaaagc gatcgggcgc
    26581 gttccgccct gctcagcatt cttgaggatc agaaacgcgc ggaagcaacc atccggtcgc
    26641 agacagaact tctgcacacc atgctgaatg ccatccccca tccgatcttc tacaaggata
    26701 cgcaggggcg ttatctggga ttcaaccgcg ctttcgagca gttctacgga aaaacaaagt
    26761 ccgaattgaa caacacaagc gtatttgaca ttgcgccccg cgagctggcc gagatatatc
    26821 atcagaaaga cctcgaactg ttcggaagcc ccggcacgca gatatacgaa accaaaatgc
    26881 tggatgcaca gggcgtcatg catgatgtcg ttgtgcacaa ggccacgttc aatgatgccg
    26941 ggggccgggt ggcggggctc gtcggcacca ttctggacat ctctgaacgc aagcggcttg
    27001 aatcagccct ggagcagaag gtcgaagagc gcacccgggc cctgaagcgc attggaaccg
    27061 agcagcagac cattctgaac tctattcgcg ccgcgattat ttacaaggac acccgcaaca
    27121 tcatcctgag ggtcaatcgt gcagcgggcg aacttctcgg cctcccggaa agtgagatgg
    27181 agggccgcag ttacgccgat ctgttccccg aggatgcggt gagccagatc gaggatgaca
    27241 tggaaatcat ccgcaccgga gccccccggt tcggcattga acagcagctg tcccttccct
    27301 cgggcggaac ccgatggatg ctgatggaca aaattccgta ccgcgatgaa acggacaccg
    27361 tcatcggcgt tatcgtgatg ggcattgaca taaccaaccg caaggaggcc gagcagagcc
    27421 tccgccagcg gagcgcggat ttgcggacca tggtcagcgc catggccaat cgcgaactcc
    27481 ggatgattga actgaaagag gaaatcgagc gcctcaggaa acaaagcccg gaaggaaccg
    27541 catgatccgg cagcggtatt tcaacgcgca tacagcatga acacggaaca gaacagactg
    27601 cccgtcagca gtgaagcggc aaaggggaat gccgaccggt ttcttttcgg ggcgctgatt
    27661 ctgctgatgt tcgccggatt ctggaccgcg cgggagatcg gccagcgggc cgatcgggcg
    27721 cttcggaatg aaatacttct atccgccgga cgcattgccc gggccatcca tccggacaga
    27781 gtccggcaac tcaccttcac cgccaccgac accaataatc cggcccacct tcgcctgcgg
    27841 acccagatga gggcctatgc cgaagccatg ggcctgcgga gcctctacag catggcgctg
    27901 cgggacggca agctggtctt cgggccggag agcctggacc cgaatgaccc gtatgcatcc
    27961 gccccgggca cggtctacga gaatccgtcg cctatggatt ttgaagtctt tcagaccggg
    28021 caggcgaatg tgcagggccc gcgaaccgat gaatacggct cgtttgtttc agcgctggct
    28081 cctgtcatcg atccccgaac cggggaagtg ctgatggcgg tgggcattga tcttgaaact
    28141 cccatgtgga agaccgctgt gcggagaacg cagctgattg tgattgagat tctaatcctg
    28201 cttttcgttg ttctggccgc aggggctctc gcggttcgtg tgcgccagcg gtataccgtg
    28261 cggaggcatc cgctgctgat ctatgcggaa accgcggtct gcgcgctgat tctgctcata
    28321 gcaaccgtcg tggccgcccg cctgacgcac caggcggagg agcgggaacg aactgagtca
    28381 ttccgctttc tcgcccaggc ccgagcggcc gcttattccc agaccttctc ctatctccgc
    28441 aatgaactga acatgctctc gggattcttt aattccagtg aacatgtctc ccgaagcgaa
    28501 ttcaactcat actgccagcc gctggtggac agcaggctga ttcaagcctg tctgtggatt
    28561 ccctcggtgt cttctgtgga aaaagacgtg ttcacggata aagcgcgagc cgaagaactg
    28621 cacggattct cgatatggga acccggtccc gacgggagcc cggcaccggc ggcagagcgc
    28681 gatattcact atccggtgct gtatgtcgaa ccgcgggatg atcatcagcg gctcacgggt
    28741 tatgacatgt cttctgatcc gctgagacga agcgccattg atgccgccct gcaaaccgga
    28801 catccctcgg catccgatcc cgttactctt tttgccgcgc ctggatcgcc gcggggattt
    28861 gttgttttcc agccggcctc caatgccggg cagaccggga cagtcggcat gggaattgtc
    28921 ccggatgcaa tcgttcagcg ccgcgcacac gtcagcgata atgcagaaat gacgttgtcc
    28981 gcaagcctgt atcatctcgc gcccgggcga ccgcccctgt ggcttgcctg ttcacgcgga
    29041 tgcgctcatc cccacgaccg ggccgcgctg cgccatgcca tccatgaatc catgcccgtg
    29101 ttcatcttcg gccaggtttt tgaagtcctg attggccccg aaccggaatg gctggcgaca
    29161 catcccctgc ggcgctggat tgccacactg cttgccggcg gcgcgctgac cttcctgttc
    29221 agcgcgctga tgggcattgc cgccggaagg cagacccggc tggaacacgc cgtgtgggag
    29281 cgcaccgcgg agctggcaaa gagtcaagat cgcttttcca gccttttccg caacctgtct
    29341 tcaagctttg cccttcatga gatgatctat gaccggaagg gaaggccctg cgactaccgt
    29401 ttcctcgaag tcaatccggc gtttgagaaa atgaccggac tgaaggccag aaacatcatt
    29461 ggccggacgg tgcgggaaat tcttccggag acggaggatg tctggattga gcgctacggc
    29521 cgcgtcgccc tgacggggcg tccggagcag ttcgaaatgt tcgcggcctc tctgaacagg
    29581 cactactccg tgatggcata ccggaccgga cccgaccagt tcgccaccct cttcgacgat
    29641 atcacggacc gccggaaggc tgaagaggac atgcgccgtc tgcaggaaat cgtcacggga
    29701 agccccaacg tggcctatct ctggcgggcc gaaccgggct ggcccgtgga atatatttca
    29761 gaaaatgtcc ggcagtacgg gtacagcccg gaagatttca ccggggggcg gatacgtttt
    29821 gatgaggtga ttcacccgga tgattttgaa cgcgttgtcc gcgagcttga agaatacagc
    29881 gcggatgaaa atttgacgga gttcaaacag gaataccgca tactggcaaa agacggccgg
    29941 attcactggg tgcacgatga cacccgcatc ctcagggact catgcggccg gatcacccat
    30001 taccagggcc tgatcgtgga tatcaccgcg ctgcgcaagg ccgaagccgc gcatgcggaa
    30061 agcgaagagc ggtaccgggc gctgatcgag gcccaggccg acgcggtctg ccgctggctg
    30121 cccgacacca ctctcaccta tgtaaaccat gcctactgcg aattctacgg caggaccaga
    30181 gaggaactgc tgggttcaaa gtggatcgaa cgtgtggcgg aatcggcacg ggaggaaatc
    30241 ctcagcacct atgcagaact cgtggcggat cccaagccgt ttatgtatga acatgaatcc
    30301 gtggccgccg acggaagtct ctgctggcag cagtggattg acacgcccct tttcgataaa
    30361 accggccgct tggtcgaatt ccagtccgtc ggccgggaca tcacgaagcg caagcatgcc
    30421 gaagttgaac tggagcggca ccggcagcgg ctggaggaac tggtgcagga gagaacgtcc
    30481 gagctgcaga cccgggtgga ggaagccgag cgcctgaatc acgccatggc caatctgctt
    30541 tcggatatcc agctcgcgca ggcacggctt gaacagtccc gccaggaact gcagatgtcc
    30601 aatgaaacga aggcccgctt cctggccacc atgagccatg aaatgcacac gccgctcaat
    30661 tccgtcatcg gctttgccga ggcgctggag gacggggttt acggaacgat cgacgaacag
    30721 cagcgggagc cgcttgagaa tatccgggaa agcgcgcagc agctgcaccg gctgatcaat
    30781 gatctgctga acatcacgcg gatcgagggc ggagtgatgc cgttccacgc ggacagtctc
    30841 ccgattcgac aggtgctgga ggaatgctgc atgatgctgc gcgcggccgc ccgcagccgg
    30901 cagcagcagt tgtcggcgga tttccggccg gtgccggaca gcctgcgcct gcggggcgat
    30961 gtcgtgaaaa tcaagcagat catgcatcac ctgatttcca acgccatcaa gttcaccccc
    31021 gatggcggaa gaatcgaggt gagcggagat ctggtggacg gaactgattt cgggcttcag
    31081 ggacgagccc tccgggtctc tgtccgggac accgggaagg gcctcacggc cgatgagctc
    31141 gcctgcgtgt tcggtctttt ctatcagtcg gaggctcccc tgacccgcac gcagggcggc
    31201 acgggtctcg ggcttcccat tgcgaaggaa ttcatccggc ttcatggggg gcgaatctgg
    31261 gccgaaagcg ccggtcccgg ccgcggctgt gtttttactt ttgttattcc cacccagtca
    31321 tgcgaacctt cgtgaggccc gaatgaacac aaacagcagc agcccgaaaa ttgaagaaac
    31381 cggaagcata ctggtggtgg atgatgatcc gcgcaaccgg acgcttctgc acgacctgct
    31441 ggtcagccgg ggatacgctg tctttgaggc cggcggcggc gaggaggcgc tgaagatgat
    31501 ggcggagaat ccgcccgaca cggtgctgct ggacatcatg atgaccggaa tggacggctt
    31561 tgaggtctgc cggcagatca agggcaaccc ggacacagcg catataccgg tcctgctcgt
    31621 caccgcgctc accgggagag aggaccgcct ggcgggcatt gacgccggcg gcgacgattt
    31681 catcaccaag ccggtggatc tcaaggaggt cctcctgcgc acgcggaacg ccgtccacat
    31741 gaagcagctc cgcgaccgcg tgcggaaaag tttcatcgaa ctggaaaaga tgtccctgat
    31801 gcgcgagaat ctcgtgcagt ggctggtgca cgacatgaag gcgccgctcg ggggcgtgct
    31861 ggggcatctg gaaatgctgc agatgaaaac agtcaacggt ccgctgcagg tgtatgtgga
    31921 cgaagcctgc gcggccacgc gccgcctgca cgaaatgatc ctcgccatcc tcgacgtgag
    31981 ccggctggag cgccatgaaa tgccgctcca gcggagcgta cgggatctgg cccggattgc
    32041 atccgaggca gtccggcaaa tggcttttga gatcggcgag cagaagatcg ctgtggagat
    32101 cgcggcggag cccgcgcccg cctggtgcga cggagagatt acgcggcggg tgatggacaa
    32161 cattctgagc aacgcgctga aatatacgcc cgagggtgga cgcatcgcgg tgcgcttcac
    32221 gtccgaagac gggcagacgc ggacagatat tgaagacaac ggaccgggca ttgatcccaa
    32281 atatcacgac atgatattcg agatgttcgg acaggtgcag atgcgccgcg agcagagaac
    32341 ctattccagc gggctgggac tggccttctg taaaatggcc gtcgaggcgc agggcgggaa
    32401 aatcggcgtg atcagcgaag aaggcaaagg ctccacattc tggttcacgc ttccgtccaa
    32461 tcccccggag aatgaggtat gaccgccaaa cgacgcatcc tgatcgtgga cgatgatccc
    32521 agcatccgct cgcttttttc acgcttcgtg caggacagcg gttatgattt ccgcacggcg
    32581 gccagcctcg cggccatgtg gaagatgttc cgcagccaca gcttcgacgc cgtgctgctg
    32641 gacatgaacc tgcccgacgg cagcagcctt gactgcgtgg cgcagatccg cgagcagttc
    32701 ccccacctga ccattgtgat gatcaccgcc aatgccgaac tgccgctggc cgttgaagcc
    32761 atacagcgcg gcgcagacaa ctttctggag aagcccgtca acctgcccag cctgagcgcc
    32821 tatttgaaga gcggtttcga agcgggggcg ctccgccggc acaaggtcgt gcgcgaccgc
    32881 aaaaagccgc cgtttgtccc tttccggggt tcaagttccg acatgcgcca tgcatgggaa
    32941 ctggcgggga tcgccgcgcg caacagcgcc gccgtgctca ttctcggcga aaccggaacc
    33001 gggaaagggg tctttgcgcg gtggatacac gaaaacagcg accgcgcgga gaggcctttc
    33061 gtgagcgtca actgctccgg cctccgcgga gaaatgctcg ccagtgaact cttcggccat
    33121 gtcaaaggag ctttcacatc agccgtatcc gacaaaaccg gcctgattga actcgccagc
    33181 ggaggcaccc tcttcctcga tgaaatcggc gacatggacc cggaacttca ggcccggttc
    33241 ctgaaagtca tcgaggaaaa gcgctatcgg cggctgggag aggccgaaga gcgtttcagc
    33301 gatttccggc tcatctgcgc cacccaccag gacctgcaga agcgctgccg tgaaaatctt
    33361 ttccgcgagg atctgctgtt tcgcatcaat gtttttccgc tcaccgtgcc gccggtgcgg
    33421 aaatgccggt cagacattcc cgcccttgcg gattatctca taaagagcct cgcgcccggg
    33481 cgggaagatg tgatcaccga tgaggcgctg aacctgctga aaaaatacgg atggccgggc
    33541 aacatccgcg agatgcgcaa tctgcttgag cgggctcttt tgctggccca gggcggcccc
    33601 atcgaggccc ggcatctgcc cggcctgtct gaaaacggag cggagcggcc cggcagcgaa
    33661 ttaaacctga accacctcga acaggaccgg atcaaggccg ccatgaaaca ggccggaggc
    33721 agcgccggcg aagcggcccg gcttctgggc ctttcccgcg caactcttta ccgaaaactc
    33781 aaaaaaatgc aaaaataggc gattccccct caactctttg cgatttttct caaatttccc
    33841 gaaaaaatgc ctttttttga ctccgacaag cctccaacgc tgattgtcaa tgaaagtgag
    33901 acacattttg accgatttta tcctgcctgt t
//
LOCUS       DAOQWR010000009        33216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1541_length_33216_cov_4.778294, whole genome shotgun sequence.
ACCESSION   DAOQWR010000009 DAOQWR010000000
VERSION     DAOQWR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 33216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33216
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_33216_cov_4.778294"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..822)
                     /locus_tag="PKK36_01495"
     CDS             complement(67..822)
                     /locus_tag="PKK36_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93264.1"
                     /translation="MKQKILFAALAGLCLLAAPAHALIEVEGFYWLMTPDGTVRFGSS
                     SVDGTSLDLESDMGFDSAERIPGFKLALGDVHQVGVGYFSMDIEADSRISENVRFGTA
                     TFPAGTDVASTWEGDIVQGFYRLNLGSSFARYGLLAGGMYIDFAASMEAEYGRSSQSV
                     KALMPYVGGYFMGYPLPWVGVRGSALASTWDWGDVSASMLDIELAGEFIFTPGVFVAV
                     GYRHTGIDATFDDDNVDIDVTFSGPIAYLGFEW"
     regulatory      1077..1208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1256..2479
                     /locus_tag="PKK36_01500"
     CDS             1256..2479
                     /locus_tag="PKK36_01500"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNR93265.1"
                     /translation="MTAKKEDKMKFSTIPEAIEAIRHGEIIIITDDEKRENEGDLVCA
                     TEKVTPGIVNFMAQKGRGLICVAMEPERLARLGLGRMVSEGRNQTRFGTAFMESVDAR
                     HGITTGISAFDRAKTLHILVDDNSGPEALVRPGHVFPLEAVPGGVLRRAGHTEASVDL
                     ARMAGLKPSGTICEILREDGEMARLPDLMKFAEEHKLKIVSTADLISHRRRTEKLVQL
                     EREVSLPTEAGTFRLKLYYSFIDDKHHIALVMGHPEKQKSALVRIHSECLTGDVLGSL
                     RCDCGTQLKSAMKQVAEEGHGVILYMRQEGRGIGLAHKIHAYALQDEGMDTVEANIHL
                     GFDADLRDYGSGAQMLADLGLHNIRLMTNNPRKVEGLELYGLEIVERVPIVMDCTVHS
                     ERYLKTKKEKMGHLL"
     gene            2490..3020
                     /gene="ribH"
                     /locus_tag="PKK36_01505"
     CDS             2490..3020
                     /gene="ribH"
                     /locus_tag="PKK36_01505"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNR93266.1"
                     /translation="MAHIKGEAKELASADLGGVAQVSGSMNGEGLRIGIVVSRFNESL
                     TTDLARTAIAALEKHGVQKKGILVHWVPGAYEVPAVIHKLALDKRFDALIALGVVIEG
                     ETPHADLINAEVAHYLAKTSRRCGVPVIHEVVGTRNIGQAVARCTSGETSRGWYAGCA
                     AVEMATLYRAMEKRND"
     gene            3013..3432
                     /gene="nusB"
                     /locus_tag="PKK36_01510"
     CDS             3013..3432
                     /gene="nusB"
                     /locus_tag="PKK36_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNR93267.1"
                     /translation="MTKRSESREWAVQLLFQREINREDLQDTLELFWRDKKPASKAKA
                     FTEQLVRGVEEHRKDIDELLRACATHWDVDRMGGVDRNAMRVAVYEMVHCPDIPPVVS
                     INEAVDIAKNMSSNESGRFVNGVLDAVLQKLDKKPAK"
     gene            3443..4348
                     /gene="ftsY"
                     /locus_tag="PKK36_01515"
     CDS             3443..4348
                     /gene="ftsY"
                     /locus_tag="PKK36_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNR93268.1"
                     /translation="MASWIDALVRTRRNIANAFGRILVRSDGPDVESLEDLEDILIGA
                     DIAPRLAAEWVEKLAHAPRGHANARKALIRKMLLGHLDHGPAFEWRSDGRPRSILLVG
                     VNGSGKTTTAARLAKNAAMCGLTPLLAATDTFRAAGADQLRLWGERIGCDVVAGASGA
                     DAAAVAYDALEAAIARKADTLIVDTAGRMHTQAPLLQELQKISRSLSKRMEGAPHETW
                     IVLDAALGQNALSQAKLFNDAVPLTGAVVTKLDGSSKGGFIFSIRRELGIPVLWAGLG
                     EGADDLALFNAEEYVDALLETGEKA"
     gene            4345..5436
                     /gene="ribD"
                     /locus_tag="PKK36_01520"
     CDS             4345..5436
                     /gene="ribD"
                     /locus_tag="PKK36_01520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNR93269.1"
                     /translation="MKSSRHEIFMRRALELAAKGEGLTRPNPPVGAVIVKNGRVIAEG
                     WHKKAGTAHAERVALARAGAAARGAALYVTLEPCCTHGRTPPCTDAIIAAGIRTVIVA
                     STDPNPKHRGRGFSALRRKGIGVIRGVCETEGNELIAPFAKWITTGRPMVTLKLGMSL
                     DGKIADRNRQSKWITGPESRKRVQAMRRRVDAIMAGAGTVRADNPQLQPRPACGRKPW
                     RVIVDVDGVIPAGARVLTDAAADRTVVAGRKRLAGRKAVETIRLPARNSLRHLLQELG
                     KMGILHVLCEGGGELAAGLIAEECVDRFAFFTAPVLIGGVASINSIGGRGWLMDSLPR
                     VAVTGVEQIGGDILILAKPEVLRHSARLE"
     gene            5442..6113
                     /locus_tag="PKK36_01525"
     CDS             5442..6113
                     /locus_tag="PKK36_01525"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNR93270.1"
                     /translation="MFSGIVQKKGEIVQHDLAEKWGKVVLRTDAWDQPLEIGESIAIQ
                     GICLTLTKMDHQGLHFDVLRETFERTNLGDKKTGQFLNMERSLRWGDTMGGHIVIGHV
                     DGTGRVKSIRPVGRDWAFEFTAEPRLMDGMVFKGSVSVDGVSLTIAELTEESFTIHII
                     PFTYEHTTFCDLKPGDAVNLEVDLLGKFVRRLVERGRFPGEITWEELRRTGLIQEPVS
                     AAEEI"
     gene            6126..6479
                     /locus_tag="PKK36_01530"
     CDS             6126..6479
                     /locus_tag="PKK36_01530"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNR93271.1"
                     /translation="MQRIILKSKIHRATITDANLNYEGSLGIDDDLLKAADIFPGEQV
                     HVLNLNSGSRVVTYAIRAPAGSGAIVLNGAAARLGAAGDPVIILTYAQASSAEAASWK
                     PQVIYVDSRNKMIQK"
     gene            6500..7078
                     /locus_tag="PKK36_01535"
     CDS             6500..7078
                     /locus_tag="PKK36_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNR93272.1"
                     /translation="MSTVETRRLGVLSQIAALEIPAAWAKAFGILSFTALLSLGAHVK
                     FFLPGNPVPVTLQTMFVLLAGALLGPWAGLSAVALYLVIGMCGVPFFAMSGVSGLMYF
                     TGPTGGYLAGFLLAAVTTGLAFQRTERTAVRLAVLAAASLLILLCGALWLGVLMHLPV
                     SRAWMLGVAPFLYGDALKTATAMTVCAVLRRR"
     gene            7192..7896
                     /locus_tag="PKK36_01540"
     CDS             7192..7896
                     /locus_tag="PKK36_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNR93273.1"
                     /translation="MSTNMEKLLILQERDLKILKLKREARDIPARKAEIEGRLKSHQQ
                     AFDEATHNLKSHKTSIQQVEGEIEALNQKILKFREQQLQIKDNTAYKALEKEIFATRQ
                     EIRTAEDRELVLMEEMEDLKRVIAEKEKSLKDEQAIVQKDNARLDQRAQDIEAEIHRL
                     ESDRQDLTRDIEKPLLSRYERILKHVGDYALVPIEKGACGGCHMNLPPQVIHDTQKGM
                     DVLSCNFCGRILYWAP"
     gene            7929..8291
                     /gene="rnpB"
                     /locus_tag="PKK36_01545"
     ncRNA           7929..8291
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK36_01545"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            8450..9412
                     /gene="trpS"
                     /locus_tag="PKK36_01550"
     CDS             8450..9412
                     /gene="trpS"
                     /locus_tag="PKK36_01550"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNR93274.1"
                     /translation="MRILSGIQPSGKLHIGNYFGMMKPALELQEQGEAFLFIADYHAM
                     TTVSNADQLRQGVRDVALDFLACGLNPEKTAFYRQSDVPEVTELTWLLSVITPMGLLE
                     RCHSYKDKLAKGIAPTHGLFSYPVLMAADILAVQSDIVPVGRDQKQHVEVTRDLAIKF
                     NLAYGETFKVPEPSIRESVAVVPGVDGQKMSKSYNNTIELFAPEKETKKKFMRVVTDS
                     RTLEEPKVPEECNVFALYRLFATDAQREEMAARYRAGGLGYGTVKAAAFELMMETFAP
                     FRKKREELEKNTDYVEEVLRKGAARARDEARRTLKTARRAVGLE"
     gene            9414..10166
                     /locus_tag="PKK36_01555"
     CDS             9414..10166
                     /locus_tag="PKK36_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNR93275.1"
                     /translation="MSEDYKVQLEVFEGPLDLLLYLIKKDEVDIYDIPIELITRQYLQ
                     YMELMKMLDLNIAGDFLVMAATLMMIKSRMLLPAEDRPEMEEEEEDDPRWDLVRQLVE
                     YKKFKDVAGHLQMMEFHMENVFTREGGGIQLGPDTDRALQDVSIFDLISAFDSALKRI
                     KQEDLREIFAERFTVGDKIDYIIGSIRSKKRIIFSSLFEGMATRHEIVCTFLALLELI
                     RLKQIRAEQAGRFEEIAIVATDDAELAGVVHE"
     gene            10335..10979
                     /locus_tag="PKK36_01560"
     CDS             10335..10979
                     /locus_tag="PKK36_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93276.1"
                     /translation="MQLLKFRYTEIILMIIVVVGSNMGSRADTQFVGNVYIDGNLVIT
                     NGGGEALVSGDTSAYLRVYGYGTSEVSGAAASIEGFNDGWAESASYGSVIEGCNEGEA
                     TVGSLGAGSRIEGYNAGWASVVFPGSKEQGYNLGMRDVIGRGIVIGGYNSSGGDQLAS
                     GNGSTIDGYNLGSNLVTGSGSSIEGYTASDAAQISGGTTGEAGGTKTAGMVKSG"
     gene            11092..12039
                     /locus_tag="PKK36_01565"
     CDS             11092..12039
                     /locus_tag="PKK36_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR93277.1"
                     /translation="MLSMQIVNSGITNTYTYIKDHLGSIVALVDETGTNVVESYEYDA
                     WGAVTVYDANESEISLSSVKNHFLWHGREYNWTVHYVQNGHGIYNFRNRWYDPVTGRW
                     LSKDPIGISGGLNQYVFCANNPVNFVDPDGRAPWMLIGAAIGGGLDLAVQLAQGRSFS
                     EVNWVSVGAGAASGALGAGLGANVARLTASITARAALNAVGSAAIGAGVQTACNAIEG
                     NNLGAGVGSAAVISGTFGGLGSAFGDLAERSLISVSQNSQFIQSLLDSGALQGNSLPY
                     LLPGEVLPPSTWSIVGTAIGATVSNLGPLVPASNAGSCQ"
     gene            12036..12260
                     /locus_tag="PKK36_01570"
     CDS             12036..12260
                     /locus_tag="PKK36_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93278.1"
                     /translation="MTGKQLLGVVLLGATGSLLGAILSLIGFGFGGWVLWLFGFLDKT
                     DAVFKVKIGVIAFVFVGFVLGILRAIRMVH"
     gene            12428..13120
                     /gene="scpB"
                     /locus_tag="PKK36_01575"
     CDS             12428..13120
                     /gene="scpB"
                     /locus_tag="PKK36_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNR93279.1"
                     /translation="MERIDVLPELKQIAGAILFAAKQPVTATRIREVLAQVAEEQGGP
                     YRDFASLKDADVEAAIRQIGEDLEKARLGIVLGDLAHGFRFENDVACGVWVRRFLEKG
                     KPSRLSRPALETLAIIAYRQPCTRAEIEAVRGVAVDTILRNLMELQLVRITGRSELPG
                     RPMMYGTTQTFLEYFGIKNLNDLPGITELRRPEDGRPEADEQVFEEPAADSAPAEVPE
                     DNSRAKEDDHES"
     gene            13110..>14072
                     /gene="pheA"
                     /locus_tag="PKK36_01580"
     CDS             13110..>14072
                     /gene="pheA"
                     /locus_tag="PKK36_01580"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNR93280.1"
                     /translation="MNLDQLRKEIDAADAELIALLNKRTRAALEIGKLKEKLDTEVYV
                     PAREKAVYDRIAKLNEGPLPEKAVQAIYREIMSASLALERNTRIAYLGPQATFTHRAA
                     REKFGASVDYEACATITDVFEAVQRQGANYGVVPIENSTEGAVTHTLDQFISTPLKIC
                     AEIYLPISISFMSKYPQKDIKKIYSKPEVFGQCRHWLQANMPHADLISCSSTARAAEY
                     VKDEPGSAAFASPLAAELYDLNILAPDVQDIAGNTTRFLVIAKKYGPPSGRDKTSILF
                     AVKHQAGSLFRAISVFNTHNLNMTRIESRPSRNKAWEYFFFVDFE"
     assembly_gap    14073..14082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14180..15292
                     /gene="hisC"
                     /locus_tag="PKK36_01585"
     CDS             14180..15292
                     /gene="hisC"
                     /locus_tag="PKK36_01585"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNR93281.1"
                     /translation="MTESIAKKYISGLGVYEPGRPMDEVARELGLADASEMIKLASNE
                     NALGPSPLAVRAMKKCAADMHLYPDGGGYYLRRALAQKYGLDMDQFILGDGSNELIQF
                     LAHVYLAKGSRIIMADRAFVVYKLIASAFEAETDMIPMIEFRHDLKAMLAAVRPETKI
                     IFVGNPNNPTGTMVYRDEIEEFMDKVPQNIAVVFDEAYIELLEPKQQPDTIQYIAKHP
                     HFYILRTFSKTYGLAGLRIGYGMASKENIRLLHRVRQPFNVNAMALAAAEAALGDERH
                     VRRTRKMVKKGLNQLQAAFDEIGLPWVPSLVNFILVKVDRGRQVFEELQKRKVIVRPM
                     DGYGLPDYIRVTVGTEEQNRIIIENLLDVLASRGDQ"
     gene            15292..16326
                     /gene="aroF"
                     /locus_tag="PKK36_01590"
     CDS             15292..16326
                     /gene="aroF"
                     /locus_tag="PKK36_01590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNR93282.1"
                     /translation="MIIVLKKSASEEQIRHIEEKIREWGLQPSISHGAEKTVIGVIGD
                     EAVIRTKPLEVFPGVESVMTVLKPYKLASREFHHDNTSVTIAPVTAGCAPTVIGGRQI
                     VVMAGPCSVESRESLFEISRCAQAGGAKIIRAGAFKPRTSPYAFQGLGRKGLEYLSEM
                     RRELGIPIVTEVMDTRDVEMVAEFADVLQIGARNMQNFNLLKAVGKVKVPVLIKRGLS
                     STIQELLMSAEYVLSQGNPDVILCERGIRTFEPMTRNTLDLSAVPVIKRESHLPVIVD
                     PSHATGHWDLIPSMSKAAVAAGADGLMLEIHPRPEEALSDGPQALLPDRYMNLMKELG
                     TLASALGRSI"
     gene            16331..17215
                     /locus_tag="PKK36_01595"
     CDS             16331..17215
                     /locus_tag="PKK36_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNR93283.1"
                     /translation="MMQNITIIGTGLMGGSLGLALKASGRPYHVAAWSRKESDREKAL
                     QLGVCDEVFASPEEAVASADISVFCTSVSSIVPLIEQCLGYFKNGSLVTDVGSTKADI
                     AQRCDVMFTGRSFSFIGSHPMAGSEKSGQEHATSDLYRGAVVALTPVSGPEREMNRLK
                     EFWRAAGAVPAVMDPDEHDRLIALTSHLPHLAAAALTAAAFQSGGGKAAGVLCGPGFT
                     DCTRVAGSLPALWRDIAASNTENILLALEAYIEELCGIRSLLEQREYQWLQEYLGESA
                     RAREELLSARKKTTGGLS"
     gene            17212..18537
                     /gene="aroA"
                     /locus_tag="PKK36_01600"
     CDS             17212..18537
                     /gene="aroA"
                     /locus_tag="PKK36_01600"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNR93284.1"
                     /translation="MKSPKDSVTVRAGRHFGGILQVPGDKSISHRVAMFSALAEGLST
                     VEGFLFSEDCICTYRAMEAMGAEVEREADDRLKVTGTGGTFRPPPGVLDMGNSGTGIR
                     LVAGLIAGAGIHAEMTGDASLCSRPMNRIAVPLREMGARISLLGDHGRPPVRIAPARL
                     RAIDYHLPMASAQVKSCVLLAGLFAEGTTRVTEPARSRDHTERILNMLGLPVRVDGLS
                     VALDGFGPAGPRFKGRHWIVPGDFSSAAFFIVAAAGMPGAELTVKSVGLNPRRAALLD
                     VLKRMGADIQICNLNQHQAGEPVGDVMVRGARLSGTDVFGDEVPALIDEIPILSVAAA
                     VAEGRTVIRDAAELRVKESDRIAVMVRNLRLMGVRAEETPDGMIVEGPSQLIPSQPLE
                     SFGDHRVVMSMAVLALYCKSPVCILNTTCVNTSYPTYWNHLRELGADFD"
     gene            18530..19222
                     /gene="cmk"
                     /locus_tag="PKK36_01605"
     CDS             18530..19222
                     /gene="cmk"
                     /locus_tag="PKK36_01605"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNR93285.1"
                     /translation="MTEKKHHVIAIDGPSASGKSTVSKKLAQVMGYLYVDSGALYRGM
                     TWKMLQENVDIHDTEVVIAKMDEIQWDFFIKDGSVWFSIDGTDPGQEIRGEAVRENVA
                     IIATIPEVRAFIGEQLRNMRHFGNIVMEGRDIGSVVFRDSPYKFYIDADPQERARRRC
                     KDIVQLEGQSNVEEVFNSLKRRDEIDSSRKTAPLQIVDGARVLDTTGMTIDEVVERIA
                     SALTQDGLSCEA"
     gene            19460..20146
                     /locus_tag="PKK36_01610"
     CDS             19460..20146
                     /locus_tag="PKK36_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNR93286.1"
                     /translation="MVHVVWGIIIASGKEEQLTSGVDVAFLNLAGRPLLSHALEEFER
                     CPDINGIVVVAPKERVGEVHSMAKLMGYMKLRKIAAGTVQRVSSLRMALNTLDDDVSM
                     VVVHEVSRPCVKRGLISECIKAAKRYGSGVSASRILDPVKIVPKGNKVTATLEANTAW
                     AAQTPQAYKLDVLEKALAAAQKKKKTLQDESAALELIKHEVHLVPCPPSNIRIATAED
                     LTLAEALKRL"
     gene            complement(20141..20653)
                     /locus_tag="PKK36_01615"
     CDS             complement(20141..20653)
                     /locus_tag="PKK36_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93287.1"
                     /translation="MNRSFQISAVLLLTVILGLSLAWFLQGRDERRILQRQEELVELL
                     SKTALEKELAALTRARQISILFTDPVSTGMEPYLTGSHSRQDMAGLAYHLRSMVEELS
                     IEIADHKLEIAPNRNSAVLATVARAKARSGGEWMSDLREVEMRWSKTDDGWLVNAVTF
                     TSAIKRPEGL"
     gene            complement(20650..21144)
                     /gene="lepB"
                     /locus_tag="PKK36_01620"
     CDS             complement(20650..21144)
                     /gene="lepB"
                     /locus_tag="PKK36_01620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNR93288.1"
                     /translation="MNSGFRRLIAGRSLTTTLWRAAGLAVITWILFTQVLTVCRLDGA
                     SMEPTMRDRSVRLVNLLKYRTGSPARGEVVIIRAAGRRVFYLKRILGLPGETVSFENG
                     QLFIDGEAHDETYLKDQGIWTMSPVILKSSEYFVAGDNRSVPITRHLMGTVERGRIAG
                     GLLW"
     gene            complement(21141..22844)
                     /locus_tag="PKK36_01625"
     CDS             complement(21141..22844)
                     /locus_tag="PKK36_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNR93289.1"
                     /translation="MNPELLPPPARMKTGEGRLMLQSPVLLHCSPKTRARLQHAIEPV
                     RDALDRRGLAMKIAEAPAPPAADAPGIVLLTPPGRGGEGFRLTASLRRITVAGDGWAG
                     LFYGLQTLRQILAAGNPIPSMEIEDAPSLPRRGFYLDISRGRVPKLEALKRLADRLSF
                     LKYNQLQLYVEHVFDFQFNPAIAQHCDPLTAEDIAALDEYCRERFIELVPSLTCFGHM
                     GRILSLPEYRRLAEIEFPAASWEAAAWLTRLRGATLNPRLAGSRALIGRMLDEFLPLF
                     SSDRFNMCGDETYDLGKGANAGTPVDELYTEHLAFVRKLAAQHNKRLMCWGDVLLKQP
                     RAVDRIPKDITILDWGYEPETDFLKAQLFRDAGLAVYVCPSTRSFKVLFNEIEKARAN
                     IAGYAQAAKHLGAEGMLVTDWGDMGHFNMPACSLHGMALGGTLAWNNEADSGEGFDRA
                     FDAQVFGAPACRAAELFSRAGRAAQNSWPGFLQGSMNAENAEELDDLADRAGTLRPGG
                     MVTRQDLDEIRLALQALRLRVRRTPEQVRDFADDYERIWLETSRPAGLCELLQQIRGL
                     P"
     gene            22904..23194
                     /locus_tag="PKK36_01630"
     CDS             22904..23194
                     /locus_tag="PKK36_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNR93290.1"
                     /translation="MKEDKQCICVRYTGHVQGVGFRYTVMSIAGRFPVAGFVRNESDG
                     SVRLEAEGTADELTAFINAIRQSSLQRYIMEEQISWQSAAGRFQGFTIGHSR"
     gene            23260..23982
                     /locus_tag="PKK36_01635"
     CDS             23260..23982
                     /locus_tag="PKK36_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR93291.1"
                     /translation="MKFALLGDIHSNLEALQAVLKDAAEQGVTDYACMGDIVGYNSNP
                     RECLEIIRNLKCRCVRGNHDHYCSHDENLAGFHPLAADVVDWTRKQLSDDELEFLRTR
                     KLIEPVESFTIVHSTLDTPEMWGYVFDKFEAEANFNYQTTSICFYGHTHVPLAFEKTD
                     IIRCGMYTKIRVGLGKKYFINVGSVGQPRDGDPRAAYVIYNMVDNTVELRRVPYDFTI
                     TQEKIRAAGLPARLASRLAQGR"
     gene            24005..25054
                     /locus_tag="PKK36_01640"
     CDS             24005..25054
                     /locus_tag="PKK36_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93292.1"
                     /translation="MKSAVIRVLAFAALAVQPAFAQIQFGGKFDHNRYLQYEPVTVNL
                     TVVNKTAKTLILGEVGGETRIRFDVRDTAGHYIQPRPGARAISMLVGAGQTVTTNVNV
                     RDYCRLDSVGSFTVTPMLVTPSRTYLAPKTFLDIVSGAVVSEMVLDVDGGLRSYSLRS
                     CARDRRTDLFLRIDDRENGMCYGVHLLGNVLSFQKPRMEVDEDDRLHIVHQAHPSYLT
                     HHVFSLNGSLIRRASYRRDGTDDLLDDEDHMELMAGEAVMDPPPAVSVPAPGPAASEP
                     SLLEVWEAAASAPDPVRASEVSEVDAIIQYLEADPVPVIPAPKASVPAAPPPAVSIPE
                     PAPAPLDPLQAPVPL"
     gene            25114..25201
                     /locus_tag="PKK36_01645"
     tRNA            25114..25201
                     /locus_tag="PKK36_01645"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25148..25150,aa:Leu,seq:cag)
     gene            25244..26353
                     /gene="alr"
                     /locus_tag="PKK36_01650"
     CDS             25244..26353
                     /gene="alr"
                     /locus_tag="PKK36_01650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNR93293.1"
                     /translation="MKRSWIEVDLNILQENVQAIRGAISPGTELICVVKANAYGHGAV
                     PVAQRAAQAGANWFAVAYLEEALKLRAALPAVNLLVLGVVDPSDTELLLEHKVIPIVA
                     TEEHGMALALAARKFGRDLEAHIKIDTGMGRLGLPWERAPDMIRRLAKEPGLNITGMC
                     SHFAKVEPAEPEAAGTQADRFFSIAKEAEDLLGRRLFKHLSSSRAILYQKDWDLNGIR
                     PGIILYGYGTGKKDMRFRTRPILQWKAFVMQVRSVPAGLPIGYYGTYKTRQPTDIATL
                     AVGYADGYLRILSNKGYVLVHGRRCPVVGRVSMNWIAVDVGPDSGVKRGDVAVLIGEQ
                     GNESVWANELAQKCITIPYEILTGIDSSIRRQYIY"
     gene            complement(26645..28009)
                     /gene="rimO"
                     /locus_tag="PKK36_01655"
     CDS             complement(26645..28009)
                     /gene="rimO"
                     /locus_tag="PKK36_01655"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNR93294.1"
                     /translation="MKLSKPVISLVSLGCAKNEVDSEHVLGALLQDGFVFAEDPRDAD
                     VCLVNTCGFIESARTETRETLEALVARKKNGRPRFVVALGCMVERAADYPDFADVLAV
                     CDLRVPFSDYPRLASLCLGLLNRKADGNPRADIMGNGPRLRIGLPHLASLKISEGCSN
                     GCSFCSIPRMRGRQISRPAADIISEAVSLAENGAKEIAVIAQDTTSYGKDLFGKPALA
                     GLLRDLAAALPENIWLRLMYAYPRFLSKNVLEVMAGDNRFCRYVDIPLQHISDRILKA
                     MGRGTSRRQVETQLDQIRTLLEPVAVRTTFITGFPGETDDEFEELLEFVREQQFNHLG
                     AFTYSPEPLTAAAALPDDIPQEVKNERRDRLMIEQMDISHRLLQEMEGQTVEVMIDRQ
                     VRNPDVPLPHDCIALAHTRHQSPDADGLVILRGTSDKKLMPGTVVPVRVTTAMDYDLI
                     ARPV"
     gene            28141..28893
                     /locus_tag="PKK36_01660"
     CDS             28141..28893
                     /locus_tag="PKK36_01660"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HNR93295.1"
                     /translation="MEVFRRKVPIIGSFVFLTLAAAAPAQDQGSASALQSVVHEWIAL
                     RDQISSEAREWDFQKQWHDTEIRLLESEKKHLEEDVRMLRGQMESRNAADAGLLAGIE
                     KLTAQREAWRPLLDDAEEDIRSWQRLLKNRMEFPLPASPEPEDRLQRVFSIYAEIEKL
                     QHRASTDKTVIELEGAGRRQMDVLYLGLGAAFAVAADGSAAAAGNWTETGWQWTRDDS
                     LAEDVRRAVRICEGQETPAWVQLPLELQEAVP"
     gene            28890..30191
                     /locus_tag="PKK36_01665"
     CDS             28890..30191
                     /locus_tag="PKK36_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR93296.1"
                     /translation="MRRLCCMLMLLALPAHAANVAEAIDRTQADTVKAVRELDELRSK
                     ISSERLPLAEKLSRLEKDVARLRSERTRLQSVLDSHTESGAGTELRKLEERAGLIEAL
                     LQDYRRSVEGRASAAELQYLKPDLDAADASGDPVETGRALLSLAKRWNRELAGGLSFS
                     GECLDGAGRLHQGAFTVIGPVVWFAGSGLSGLAVTAPDSMLPSLAERAPARSGVGFPV
                     DVTGGKVLKIEGSRGSWMDHLKSGGFVMFPLLLLGLLAVVIIVWKTVSLRNIRPADTT
                     LVRQIAASGESGELMERLGFPAGPIIAEGLRHRHAPPELLEEIMYERMLMHVPALERH
                     LGTLAVIGGVAPLLGLLGTVTGMIHTFRLVTLFGSGEAHLLSGGISEALVTTETGLVI
                     AIPVLIIHAYFHRRVKSIIAGLELMISEFARQTGQVKGDAE"
     gene            30188..30745
                     /locus_tag="PKK36_01670"
     CDS             30188..30745
                     /locus_tag="PKK36_01670"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNR93297.1"
                     /translation="MKWMMIAAGIMFCAACAAEEPVPSIYDFQVDDIDSNPVSMSEYR
                     GKVLLIVNVASKCGFTRQYAGLEKLYQDYQDQGLVVLGFPANSFMGQEPGTNEEIKDF
                     CTSTYGVTFPMFSKISVKGKDMAPLYRYLTDRKLNPPSEGGVTWNFNKFLISRDGKML
                     NHFRPRPGPESEELRAAVEAALETP"
     gene            30752..31339
                     /locus_tag="PKK36_01675"
     CDS             30752..31339
                     /locus_tag="PKK36_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR93298.1"
                     /translation="MQVLKHILDYWTAGGILLVPLAAVSFSIWFYFLRTRSALLAAAE
                     IPSGFEHRILETADEDRLIPGAVKYAAGAARAGRDPRQAWQEYQDAAVRGLRKDVVVL
                     AALTAAAPLIGLLGTVTGMIGTFRAVMQAHTVTGGAVADGISQALITTQFGLIIALPG
                     VFGVARLHRLIRHLNFRFAVCRSHLLLALGNGGRS"
     gene            31336..31746
                     /locus_tag="PKK36_01680"
     CDS             31336..31746
                     /locus_tag="PKK36_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR93299.1"
                     /translation="MRRDRYYDDEQAATVAINLSPLIDVVFLLLIFFVVTTTFVESTG
                     IEIDKPAAVSAENLRERSILIALAPDGQIYFGGQAVALQQVRGLVARQLQRLERPVIV
                     LADRRAQSGALIAVMDECRLAGAKKVNVATEKHE"
     gene            31739..32404
                     /locus_tag="PKK36_01685"
     CDS             31739..32404
                     /locus_tag="PKK36_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR93300.1"
                     /translation="MSELRQAVMRQAPAPGTVLLPLLLTTAVFLALPLMERITERAAD
                     YVIRPLDMIEQMPAIPEPQPVRTAVQSRPVRMEQRPELLRPSDVQMPSMPLSEMADMV
                     RLDALPVDPAMAVFDIALAAGGPVDLSALDVIPIPVSRMDPVYPASARARGIEGFVTL
                     EFVIGEDGRVSSARVTDSVPGGIFDDASVRAVGSWRFRPGEMDGRPVAVKVRQTLNFK
                     LKE"
     gene            32548..>33216
                     /locus_tag="PKK36_01690"
     CDS             32548..>33216
                     /locus_tag="PKK36_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93301.1"
                     /translation="MVYADASQPVISSETAETLNRVQSVAETNLAMAITQMENYSRDD
                     AGAAVIFTLGNLYLRDGKLDEAKAAFSQALEKHSDFEQARQNLGHVAFLQEQYDDAIG
                     IFQKLISDGKANAEIWLMLGHALVLTDHPVAAENAYRNALTLQLENPAAKQGLLRALI
                     AQERYPEASKLLDELMLKNPEAGELWTLKANLALALGKDTEALTALESARRLGVTEIS
                     VKLLL"
BASE COUNT         6904 a         9226 c        10333 g         6743 t
ORIGIN      
        1 cgctgaaaga tgccccgcgg cttgccgcgg ggagtttcac tgattggaac tttttgcccc
       61 cgccggtcac cactcaaagc caagataggc gatggggccg gagaaggtga catcaatgtc
      121 cacgttgtcg tcgtcgaagg tggcatcaat gcccgtgtgg cggtagccga ccgcgacaaa
      181 tacgccgggg gtgaagatga attcgccggc cagttcgatg tccagcatgc tggcgctcac
      241 atcgccccag tcccacgtgg aggcgagggc ggagccgcga acgcccaccc acggaagcgg
      301 atagcccatg aaatagccgc ccacataggg catcagggcc ttgacggact ggcttgagcg
      361 gccgtattca gcctccatgg aggccgcgaa gtcaatatac attcctcctg ccagcaggcc
      421 gtagcgcgcg aaactggacc cgagattgag ccggtagaat ccctgcacga tgtcgccctc
      481 ccatgtggaa gcaacatccg tgcctgcggg aaaagtggct gtgccgaagc gcacattttc
      541 ggagatgcgg ctgtcggctt cgatgtccat cgagaaatag cccactccga cctgatgcac
      601 atcaccgagc gcaagcttga acccgggaat gcgttcggcg gagtcgaagc ccatatcgct
      661 ttccagatca aggcttgttc cgtccacact gctgcttcca aaacggaccg tgccgtcggg
      721 cgtcatgagc cagtagaagc cctcgacttc aatcagcgcg tgcgccggcg cggccagcag
      781 gcacaatccc gccagtgcag caaaaagaat tttctgtttc atagtttcac cttaaccttt
      841 tctcaaaacc acagggcaca tagcatagac ccgaatcact aattttccag aagaaacatt
      901 tgggaggaaa agtccgacgc aacttctgaa attcttctct gtattcctta aaaacgccgc
      961 gaggcgtgtg caaacaatgc atgcttcctt tagatgggct tgtgagaaca gctgatttaa
     1021 gggcttgcgg tattctgcag gccggtttat gttcctgtga aattttagaa ctggtgacag
     1081 attctcagga cagggtgaaa ttccctaccg gcggtgaaag cccgcgagtc cttcagtgag
     1141 ggactgatgc gcagtctgga aacggataaa cgcagccgac agtaaagtct ggatgggaga
     1201 gagtttgcga ggattctttc ttgttcccgg aggtctgtgc acctccggtt tttttatgac
     1261 cgcaaagaaa gaagacaaaa tgaagttcag cacgatcccg gaggcgattg aggccatccg
     1321 gcacggtgag attatcatca ttaccgatga cgaaaagcgc gagaacgagg gcgatctggt
     1381 ctgtgccact gaaaaggtca cgcccggaat tgtgaatttc atggcgcaga agggccgcgg
     1441 tctgatctgc gtggccatgg agccggagcg gctggcgcgg ctgggcctgg gacgcatggt
     1501 cagcgaggga cgcaaccaga cccgcttcgg caccgccttc atggagtccg tggacgctcg
     1561 ccacggcatc acgaccggca tcagcgcatt tgaccgcgcg aagaccctcc atatccttgt
     1621 ggatgataat tcaggtcccg aggccctggt gcggcccggg catgtgttcc cgctggaggc
     1681 ggtcccgggc ggggtgctgc gccgcgcggg acacactgag gcttcggtgg atcttgcccg
     1741 catggcaggg ctgaagccct ccgggaccat ctgcgaaatc ctgcgcgagg acggcgagat
     1801 ggcccgcctg ccggatctga tgaaattcgc ggaggagcac aagctgaaaa ttgtttccac
     1861 cgccgatctc atttcgcacc gccggcgcac ggagaagctc gttcagcttg agcgcgaagt
     1921 ttccctcccc acggaggccg gaactttccg tctgaagctt tactattcct ttatagacga
     1981 caagcatcac atcgcgctgg tgatgggcca tccggagaag cagaagtccg cgctggtccg
     2041 gattcacagc gagtgcctca ccggcgatgt gcttggctcg ctgcggtgcg actgcggcac
     2101 gcagctgaag tccgctatga agcaggttgc ggaggagggg cacggtgtca ttctttacat
     2161 gcggcaggaa ggccgcggca tcggcctggc gcacaagatt cacgcctatg cgctgcagga
     2221 tgaggggatg gacactgttg aggccaatat ccacctcggc tttgacgccg acctgcgcga
     2281 ttacggcagc ggggcacaga tgctggccga tctcggtctg cataatatcc gcctgatgac
     2341 caacaaccct cgcaaggtcg aaggccttga gctgtacggt ttggaaattg tcgaacgcgt
     2401 gccgatcgtg atggactgca cggtccacag cgaacgctat ctcaaaacaa agaaagaaaa
     2461 gatgggacat ctgctctagg agaaatttca tggcgcatat aaaaggtgaa gcaaaagaac
     2521 tggcctccgc tgatctgggc ggcgtggctc aggtctccgg atccatgaac ggcgagggac
     2581 tcaggatcgg cattgtcgtc agccgcttca acgagtcgct gacaacggac ctggcccgca
     2641 cggcgattgc cgcgctggaa aagcacggtg tgcagaagaa gggcattctg gtccactggg
     2701 tgccgggggc ctatgaagtc cccgccgtga ttcataagct ggcgctcgac aagcgctttg
     2761 acgcgctgat cgccctgggc gtggtcatcg agggtgaaac gccccacgcc gacctgatca
     2821 atgccgaggt tgcgcattac ctggccaaga catcccggcg gtgcggcgtg ccggttattc
     2881 acgaggtggt gggaacacgc aacatcggcc aggccgtggc ccgctgcacc agcggcgaaa
     2941 caagccgcgg ctggtatgcg ggatgcgccg cggttgaaat ggccacgctt tatcgcgcaa
     3001 tggagaagag aaatgactaa acgatccgaa tcgcgcgagt gggccgtcca gcttctgttt
     3061 cagagggaaa tcaatcgtga agatctgcag gacaccctcg aactcttctg gcgagacaaa
     3121 aaacccgcct ccaaggcgaa agctttcacg gaacagctgg tccgcggcgt ggaagagcac
     3181 cggaaggata tagacgaact cctgcgcgcg tgcgcaacgc actgggatgt ggaccgcatg
     3241 ggcggcgtag accgcaatgc catgcgcgtg gcggtctacg agatggtgca ctgcccggat
     3301 atcccgccgg tggtttcaat caacgaggcc gtggacatcg ccaagaacat gagcagcaat
     3361 gaatccggcc gttttgtgaa cggcgtgctc gacgcagttc tgcagaaact cgacaagaag
     3421 cccgcgaagt gaggaagaag gcatggcaag ctggatagac gccctggtca gaacccgccg
     3481 aaacatcgca aatgccttcg gccggattct ggttcgaagc gacgggccgg atgtggaatc
     3541 gctggaggat ctggaggata tcctcattgg agcggacatc gcaccgcgtc tggccgccga
     3601 gtgggtggag aagctcgcgc atgcgccgcg cggccatgcg aatgccagaa aagccctgat
     3661 ccgcaagatg cttctgggcc acctggatca cggaccggcg ttcgaatggc gctccgacgg
     3721 ccggccgcgt tccatccttc tggtcggcgt gaacggttcc ggcaagacta ccacggcggc
     3781 ccggctggca aaaaatgcag cgatgtgcgg actaaccccg ctgctggccg caacggatac
     3841 gtttcgcgcg gcgggcgccg accagctcag gttgtggggg gaacggatcg gatgcgatgt
     3901 ggttgcgggg gcctcggggg ctgacgcggc cgcagtggcg tatgacgctc tcgaagcggc
     3961 gattgcccgc aaggccgaca cactgattgt ggataccgcc ggccgcatgc atactcaggc
     4021 gccgctgctg caggagctcc agaaaatcag ccgttccctc agcaagcgca tggagggcgc
     4081 tccgcacgag acctggatcg tgctcgacgc cgcattgggc cagaacgccc tcagccaggc
     4141 caaactcttc aacgatgctg ttccgctgac cggcgcagtg gtgaccaagc tggacgggtc
     4201 ttcgaagggc ggtttcattt tttccatccg gcgggaactg ggaattcccg tgctgtgggc
     4261 cgggctggga gagggggcgg acgatctggc gctgttcaac gcggaggagt acgtggatgc
     4321 gcttctcgaa accggggaaa aagcatgaaa agcagccggc atgaaatatt catgcgccgc
     4381 gcgctggaac tcgcagccaa aggcgaaggc ctcacccggc ccaacccgcc tgtgggggcc
     4441 gtgatcgtga aaaacggccg cgtcattgca gagggctggc acaaaaaggc ggggacggca
     4501 cacgcagaac gcgttgctct ggcccgggcc ggagccgccg ccaggggcgc cgcgctttat
     4561 gtaacgcttg agccctgctg cacgcatggc cgcacgccgc cctgcacgga tgcgatcatc
     4621 gccgccggta tccgcaccgt gatcgttgct tcgactgacc cgaacccaaa gcaccgcggc
     4681 aggggttttt ctgcgctgcg caggaagggc atcggggtga tccggggagt ctgtgagacg
     4741 gagggcaatg agctgattgc gccgtttgcg aaatggatca ccaccgggcg gccgatggtt
     4801 accctgaagc tgggcatgtc gctggacggg aaaattgccg accgcaacag gcaatccaaa
     4861 tggatcacgg gccccgagtc ccgaaaacga gtgcaggcga tgcgccgccg ggtggatgcc
     4921 attatggctg gcgccgggac cgtcagggcg gataacccgc agctccagcc gcgcccggcc
     4981 tgcgggcgca agccgtggcg ggtgattgtg gatgtcgatg gagtgatccc tgccggcgcg
     5041 cgggtgctga cggacgccgc cgcggaccgc accgtcgttg ctggacgaaa acggctcgcc
     5101 gggagaaagg ctgtagaaac gattcgcctg cccgcccgaa attcgttaag acatctgctc
     5161 caggaactcg gcaaaatggg catcctgcat gtgctgtgcg aagggggcgg ggaactggcc
     5221 gcgggtctca tcgcggagga atgtgttgac cggttcgcgt ttttcacggc ccccgtgctg
     5281 atcggcggcg tcgcttccat caacagcatc ggcggaagag gctggctcat ggacagcctg
     5341 ccgcgtgtgg cggtgaccgg cgtggaacag atcggcggcg atattttaat tctggcaaaa
     5401 ccggaagttt tgcggcacag tgcgcggctg gagtagaaat catgtttagt gggattgttc
     5461 agaaaaaagg tgaaattgtt cagcatgacc tcgccgagaa gtggggcaaa gtggttctgc
     5521 gcacggatgc atgggaccag ccgctggaaa tcggcgagag catcgccatc cagggcatct
     5581 gcctgacact gacgaaaatg gaccatcagg ggctccattt tgatgtgctg cgcgaaacgt
     5641 tcgaacgcac caatctcggc gacaagaaga ccggacagtt tttgaacatg gagcggtcgc
     5701 tccgctgggg cgacacaatg ggcggccata ttgtgattgg ccatgtggac ggcacgggcc
     5761 gtgtgaaatc catccggccg gttggccgcg actgggcctt tgagttcacg gcggagcccc
     5821 ggctgatgga tggaatggtg ttcaagggtt cggtttcagt ggacggtgtg agtctcacga
     5881 tcgcagagct gaccgaggaa tcgttcacca ttcacatcat tcctttcacc tacgaacaca
     5941 ccactttctg cgacctgaag ccgggcgatg cagttaatct tgaagtggac cttctgggca
     6001 agtttgtgcg gcgactggtt gagcgcggcc ggttcccggg agaaatcacg tgggaagagc
     6061 tgcgcaggac cggcctgatc caggagcccg tttctgccgc ggaagaaatt tgaacaagga
     6121 cgaaaatgca gcgcatcata ctgaaatcaa aaatccacag agccaccatt acggatgcca
     6181 atctcaatta tgagggaagt ctgggcatcg acgacgattt gctgaaggcg gccgacattt
     6241 ttcccggtga acaggttcat gttctgaatc tgaacagcgg aagccgtgtt gtcacctatg
     6301 ccatacgggc gccggccggg tcgggagcca ttgttctgaa cggcgctgcc gcaaggctgg
     6361 gcgccgcagg cgatccggtg atcattctga cttatgccca ggcctcatcg gccgaggccg
     6421 cgtcatggaa gcctcaggtg atttatgttg attcccggaa taaaatgatt cagaaatagc
     6481 gtctcgaatg gaggttcaaa tgagcacagt tgaaacgaga agactgggag ttctttctca
     6541 aatcgccgcg ctggaaattc cggccgcctg ggcaaaggcc ttcgggatac tgtcattcac
     6601 agccctgctg tccctgggcg cgcatgtgaa attctttctt ccgggcaatc ctgttccggt
     6661 gactcttcag accatgttcg tgctgctggc cggcgcgctg ctgggcccct gggcaggact
     6721 gtccgcagtg gcgctttatc tcgtcattgg gatgtgcggc gttccgtttt ttgccatgtc
     6781 cggggtctcc ggcctgatgt attttaccgg gcccaccggc ggctatctcg cgggatttct
     6841 gctggccgct gtaacgaccg ggcttgcttt ccagaggacg gaaagaacgg cggtgaggct
     6901 tgccgttctt gcggccgcct cgctcctgat cctgctgtgc ggagcgttgt ggctgggtgt
     6961 gctgatgcat ctgccggtca gccgggcatg gatgctgggc gtggcgccgt ttctttacgg
     7021 cgatgcgctc aaaaccgcga ccgcgatgac tgtttgcgca gtcctgcgcc gccgctgatc
     7081 agcgcccgtt tttaatcgca gcaatctgtt gactctcccg gggagcgtca ctacactacc
     7141 cgtgcttttt gatatttgca cattcagtcg gcgcgacaga taaggaatac gatgtcaaca
     7201 aacatggaaa aactgctgat tctgcaggag agggacctca aaatcctcaa gctcaagcgg
     7261 gaagcgaggg atatccccgc ccggaaggcc gaaatcgagg gacgcctgaa atcccatcag
     7321 caggcttttg atgaagcgac gcacaacctg aaaagtcaca agacatccat tcagcaggtg
     7381 gaaggcgaga tcgaagcgct caatcagaaa attctcaaat tccgcgagca gcagctccag
     7441 atcaaggaca acacggccta caaggcgctg gaaaaggaga tttttgcgac tcggcaggaa
     7501 attcgcaccg cggaggaccg ggaactggtc ctgatggagg agatggagga tttgaagcgc
     7561 gtcatcgccg agaaggagaa atccctgaag gacgagcagg ccatagtgca gaaggacaat
     7621 gcccgcctgg atcagcgcgc acaggacatc gaagctgaaa tccacaggct ggaatccgac
     7681 cggcaggacc tcacgcggga tatagaaaag ccattgcttt cacggtatga acgcattctg
     7741 aagcatgtgg gtgactatgc ccttgtgcct atcgagaagg gggcttgcgg cggatgtcac
     7801 atgaaccttc cgccgcaggt gatccacgac acgcagaagg gcatggatgt cctgtcctgc
     7861 aatttctgcg gccgcatctt gtactgggcg ccctgacggc gtatattcca gcgttctttg
     7921 aaagtttcac gcggagacga ttggtcgctc gttcgcagga acgggaggaa agtccggact
     7981 ccacagagca ggatgaccct gtgaaaacgg ggaagctctt cgccatatcg aagagtatgg
     8041 aaagtgccac agaaaacaaa ccgccacgga gcaatccggg gcaagggtga aaaggcggtg
     8101 taagagacca ccgccccgag agagatcgag ggggcagggc aaaccccatc cggagcaaga
     8161 tcaaataggg aactggaggc tgctcgcttc attttgcgca agcaatgcag ttccgggttg
     8221 atcgctcaga caaatgacca atgcctgcag caatgcagga acagaatccg gcttacaggc
     8281 tccgcgtaat gaacattccg gccccgctga cggggccggt tttcatttgg actcctgcgt
     8341 tgactcagca ttccgccggt ttgagcctct gcggggaatt tttcttttga atcctgccct
     8401 gttctgggat agtttctgca tttcgcgatt taatctgagg tgaatgatca tgagaatttt
     8461 gtcggggata cagccctccg gaaaacttca tatcggcaac tatttcggga tgatgaagcc
     8521 cgcgctcgag ctccaggagc agggcgaggc gtttcttttc atcgccgact accacgccat
     8581 gaccacggtg tccaatgcgg atcaactccg gcagggggtt cgcgatgtgg cgctggattt
     8641 tttggcttgc ggattgaatc ccgaaaagac ggcattctac cgccagagcg atgtgccgga
     8701 agtgacggag ctgacgtggc ttctttccgt gatcacgccc atgggcctgc tggagcgttg
     8761 tcattcttat aaggacaagc tggccaaagg catcgcgccc acgcacggcc tgttttcgta
     8821 cccggtgctt atggcggcgg atattcttgc cgtgcagtcg gatatcgttc cggtgggccg
     8881 tgaccagaag cagcatgtgg aagtgacccg tgatctggcg atcaaattca atctggccta
     8941 cggagagacc tttaaggtgc cggagccgtc catccgcgag agcgtggcgg tggtgccggg
     9001 cgtggacggg cagaagatgt ccaagtccta caacaacacg atcgagcttt tcgcgccgga
     9061 aaaggagaca aagaagaagt ttatgcgggt tgtgacggac agccggactc tggaggaacc
     9121 caaggtgccc gaggaatgca acgtgtttgc gctgtaccgt ttgttcgcga cggatgcgca
     9181 gcgcgaggaa atggccgcgc gctaccgcgc tggcggcctc ggttacggca cggtcaaggc
     9241 ggccgccttt gaactgatga tggaaacctt tgctccgttc cggaagaaac gcgaagaact
     9301 ggagaagaat acggactatg tcgaggaagt tcttcgcaag ggcgccgcac gcgcgcgcga
     9361 cgaggcccgc cgcacactga agacggcccg ccgggccgtg ggactggagt aacatgtccg
     9421 aagactacaa agttcaactc gaagtattcg aggggccgct cgacctgctg ctctacctca
     9481 tcaagaagga tgaggtggac atctacgata ttccgatcga gctgatcacg cgtcagtatc
     9541 tccagtacat ggagctgatg aagatgctgg atctgaacat cgcgggtgat ttcctcgtga
     9601 tggcggccac gctgatgatg ataaagagcc gcatgctgct cccggctgag gaccgccccg
     9661 agatggagga ggaagaggag gacgatcccc gctgggatct ggtgcgccag cttgtggaat
     9721 acaagaagtt caaagacgtg gccgggcatc tccagatgat ggaattccac atggagaatg
     9781 tcttcacccg cgagggcggg ggaatccagc ttggccccga caccgaccgc gccctgcagg
     9841 atgtgagcat cttcgatctg atctccgcat tcgacagtgc cctcaagcga atcaagcagg
     9901 aagacctgcg ggagatattc gccgagcgct tcacggtcgg cgacaagatt gattacatca
     9961 tcggaagcat ccggagcaaa aagaggatca tattcagcag cctgttcgag ggcatggcca
    10021 cgcggcacga gattgtatgc accttcctcg cgctgctgga actgatccgc ctcaagcaga
    10081 tccgggccga gcaagccggg cgctttgagg agattgcgat tgtggcgacg gatgatgccg
    10141 aactggcagg agtggtacat gaatgaaata atggttcttc ctgaactcaa acagatcgct
    10201 ggagcagtcc tgttcgcggc caaacagccg gaattgttcg cggagatata ttgtttcagc
    10261 cgaaaagata actgctaacc aattgatatt tcccagcgag gcttctagtg tgtacttttg
    10321 acaaggagat gaccatgcaa ctgcttaagt ttcgatacac agaaatcatt ttaatgatca
    10381 ttgttgttgt cggctccaac atgggcagcc gcgctgacac gcaattcgtt ggaaacgtgt
    10441 acatagacgg caaccttgtc ataacaaacg gcggaggaga agctttggtg tctggtgata
    10501 ccagcgctta tcttcgggtt tacggatatg gcacctcgga agtgtccgga gccgctgcaa
    10561 gtatagaagg tttcaatgat ggttgggcgg aatccgcaag ctatggttct gtaattgaag
    10621 gatgtaacga gggggaggca actgttggaa gtctgggggc cggatcccgc attgagggat
    10681 ataatgcggg ctgggcttcg gtggtattcc ctggttcaaa agagcagggc tataatctcg
    10741 ggatgaggga cgtgattggc aggggcattg tcattggcgg atataatagt tcaggaggag
    10801 atcaacttgc cagtgggaac ggatcaacca ttgacgggta caatctcggt tcaaatctgg
    10861 ttaccggttc tggttcctct atcgaaggtt acacggcatc ggatgctgca cagatttccg
    10921 gaggaacaac aggcgaagca ggcgggacga aaaccgcggg catggttaaa tctggttaaa
    10981 tccacagggt ccaacacgta ttcagaggta tatgacggat ataatccagt atacagagat
    11041 tacggttctg gtaataaaat taaatataca cagggaccgg gaattgataa tatgctgagc
    11101 atgcagattg ttaatagcgg aataacaaat acttatacct atatcaaaga tcatctgggc
    11161 agtattgtag cgctggtcga tgaaaccgga accaatgtgg tggaatccta tgaatacgat
    11221 gcatggggtg ctgtaacggt atatgatgcc aatgagtctg aaatttcctt gtcttctgtc
    11281 aaaaaccatt tcttatggca tggcagagaa tacaactgga ctgttcacta tgttcagaat
    11341 gggcatggca tttacaactt ccgcaatcgc tggtatgacc ccgtcactgg gcgatggctt
    11401 tccaaagacc cgattgggat aagcggcgga ttgaaccagt atgtcttctg cgccaataac
    11461 cccgtcaact ttgttgaccc ggacggccgg gcgccatgga tgcttattgg tgccgcgatt
    11521 ggtggaggtc tcgatttagc cgttcaactc gcgcagggca gaagttttag tgaagtgaat
    11581 tgggtttctg ttggcgcagg cgctgcatcg ggcgcccttg gtgccggttt aggtgccaat
    11641 gttgcccgtt taactgcatc catcacggca agagccgcac tcaatgcggt tggcagtgca
    11701 gccataggtg cgggtgttca aactgcttgc aacgctatcg aggggaataa tctaggcgca
    11761 ggtgttggaa gcgcggcagt cattagtgga acgtttggcg gtctcggatc agcctttggc
    11821 gatctggcag agcggagcct catttcggtc tcccaaaact cgcagttcat tcagagtctt
    11881 ctcgacagtg gtgctttaca ggggaatagc cttccatacc tattgccagg cgaagtcctc
    11941 cccccttcga cttggtctat cgtcgggact gctatcggtg ccaccgtctc gaatctcggg
    12001 cctctggttc cagcaagcaa tgcggggtcg tgccaatgac gggcaaacag ctacttgggg
    12061 ttgttctgct gggtgcgacc ggaagtctgc ttggcgctat tctatccctg atcggtttcg
    12121 gtttcggcgg ttgggttctt tggctatttg gtttcctcga taagacggat gctgtgttca
    12181 aggtcaaaat aggagtgatt gcgtttgtgt ttgtcggttt tgtgttaggt attcttcgcg
    12241 cgattaggat ggtccattga cgtgcgcccc ctcgctacgc cggtgatcac ggcgtctgct
    12301 tacgcacacg ccgcggatag gggtcaggtc ttaacattta acatttgaat ctcgaaaaga
    12361 tggcggtttc agagagacgg attttaacat taataagtaa tttgataagt ggtgctggag
    12421 aaataacatg gagcggatag atgttcttcc tgaactcaaa cagatagccg gggcgatatt
    12481 gttcgcggcc aaacagccgg tgacggccac gcgcatccgc gaggtgctcg cgcaggtggc
    12541 cgaggagcag ggcggcccgt accgcgactt cgcgtcgcta aaggacgcgg atgtcgaggc
    12601 ggctatccgc cagattggcg aagaccttga gaaggcccgc cttggtattg tgctgggtga
    12661 ccttgcgcac ggcttccgtt ttgaaaacga cgtagcctgc ggcgtatggg tgcggcgctt
    12721 tcttgaaaag ggcaagccca gccggctttc gcggcccgcg ctggagacgc tggcgatcat
    12781 cgcctaccgc cagccctgca cccgcgccga aatcgaggcg gtgcgcggcg tcgcggtgga
    12841 caccattctg cgcaacctga tggaactgca gcttgttcgg atcaccggcc ggagcgaact
    12901 ccccggccgg ccgatgatgt acggcacaac ccaaacgttt ctggaatact tcggaatcaa
    12961 gaacctgaat gatctgccgg ggatcacgga gctccggcgc cctgaggacg gcagaccgga
    13021 ggcggatgag caggttttcg aggagcctgc ggcagattcc gctccagcgg aggtccctga
    13081 agacaacagc agagcgaagg aggatgacca tgaatcttga tcaactgcgc aaggaaattg
    13141 atgcggccga tgccgagctg attgcactgc ttaacaagcg cacccgcgcg gcgctggaga
    13201 ttggcaagct caaggagaag ctggatacag aggtttatgt gccggcccgt gagaaggcgg
    13261 tctatgaccg cattgcaaag ctcaacgaag ggccgcttcc ggaaaaggcc gtgcaggcga
    13321 tctaccgcga aatcatgtcc gcttcgctgg cgctggagcg gaacacgcgg attgcatatc
    13381 tcggtccgca ggccacgttc acccaccgcg ccgcgcgcga gaaattcggc gccagcgtgg
    13441 attatgaagc ctgtgccacg atcacggatg tttttgaggc tgtgcagcgc cagggcgcca
    13501 actacggggt ggtgccgatc gaaaattcga ccgagggggc ggtgactcat acgctggatc
    13561 agttcatatc cacgcccctg aagatttgcg cggaaatcta tcttccgatc tccatcagct
    13621 tcatgtccaa atatccccaa aaggacataa agaaaatcta cagcaagccc gaggtgttcg
    13681 gtcagtgccg gcactggctt caggccaaca tgccccatgc agacctgatt tcatgttcca
    13741 gcaccgcgcg cgcggcggaa tatgtgaagg acgagcccgg cagtgcggcc ttcgcctcgc
    13801 cactggcggc ggaactttat gatctgaaca tccttgctcc cgatgtgcag gacatcgccg
    13861 gcaacaccac gcggtttctg gttatcgcga agaagtacgg tccgccctcc ggtcgagaca
    13921 agacctcgat actgttcgcg gtgaagcatc aggccggctc gctgttccgg gccatttcgg
    13981 ttttcaacac ccacaatctg aacatgaccc gcatcgaatc ccgcccgagc cgcaataagg
    14041 catgggaata tttctttttt gtggatttcg agnnnnnnnn nngaacccaa cgtgggcaag
    14101 gccatgggcg cacttgcgga gcagtgcatc gaactctccg tcctgggctc ttatccgcag
    14161 gcgggaaggg aaagctgaaa tgacagagtc tatcgcaaaa aaatacatca gcggcctcgg
    14221 ggtctatgag ccgggccgtc cgatggatga ggtcgcgcgg gaactcgggc ttgctgatgc
    14281 gtcggaaatg atcaagctgg cgtcgaatga gaatgcgctg ggcccgtctc ctctggcagt
    14341 cagggccatg aaaaaatgcg cggccgacat gcatctttat cccgacggcg ggggatacta
    14401 tcttcgccgg gcgctggcgc agaagtacgg actggacatg gaccagttta tcctcggcga
    14461 cggcagcaac gaactgatcc agttcctcgc gcatgtctat ctggcaaagg gctcgcgcat
    14521 cattatggcg gacagggctt ttgtggtcta caagctgatt gcctccgcct ttgaggccga
    14581 gacggacatg attccgatga ttgaattccg gcacgatctg aaggctatgc tggccgccgt
    14641 gcggcccgaa acaaaaatca tctttgtcgg gaatccaaac aatccgaccg gaacgatggt
    14701 gtaccgggac gaaatcgagg aattcatgga caaggttccg cagaacattg cggtggtgtt
    14761 cgatgaggcc tacattgaac tgctggagcc gaagcagcag ccggacacca tacagtacat
    14821 tgcgaagcat ccgcatttct acattcttcg cacattctcc aagacatatg gcttggccgg
    14881 cctgcgcatc gggtacggca tggcatcgaa ggagaacatc cggctgcttc atcgtgtgcg
    14941 ccagcccttc aacgtgaacg cgatggcgct ggccgcggcc gaggccgcgc ttggagacga
    15001 acgtcacgtc aggcgcactc gcaagatggt caagaagggg ctgaaccagc tgcaggcggc
    15061 ttttgatgaa attggtctgc cgtgggttcc gtcactggtc aacttcatcc ttgtgaaggt
    15121 ggaccgcggc aggcaggtct tcgaagaact gcagaagcga aaagttattg tgcgcccgat
    15181 ggacggctat gggcttcccg actacattcg cgtgacggtt ggcacagaag aacagaaccg
    15241 gattatcatt gagaatcttt tggacgttct ggcctcgcgg ggagatcaat aatgatcatt
    15301 gttctcaaga agagcgcctc cgaagagcag atccgccaca tcgaggagaa aatccgcgaa
    15361 tgggggcttc agcccagcat cagccatggt gcggagaaga cggtgatcgg tgtaatcggg
    15421 gatgaggcgg tcatacgcac gaaaccgctg gaggtgtttc ccggcgtgga atcggtgatg
    15481 accgttctga aaccctacaa gctggcgagc cgtgaatttc atcacgacaa tacatccgtc
    15541 acgattgccc cggtaacggc tggttgtgca ccaacggtta taggcgggcg gcagattgtg
    15601 gtcatggcgg ggccctgctc ggtggaaagc cgcgagtcgc tgtttgaaat ctcgcgctgc
    15661 gcccaggccg gcggggcgaa gattattcgg gcgggggctt tcaagccccg tacctctccg
    15721 tatgcatttc aggggctggg gaggaagggg ctcgagtatc tttccgaaat gcgccgtgaa
    15781 ctgggcattc caatcgtgac ggaggtgatg gacacgcgcg atgtcgagat ggttgcggaa
    15841 ttcgcggacg tcctgcagat cggcgcgagg aacatgcaga atttcaatct gttgaaggct
    15901 gttggaaagg tgaaggttcc tgtgcttatc aagcggggac tttccagcac gatccaggag
    15961 ctgctgatga gcgcggaata tgtgctgagc cagggcaatc cggatgtcat tctctgcgag
    16021 aggggtatcc gcacgttcga gcccatgacc cgcaacacac ttgatctgag cgccgttccg
    16081 gtgatcaagc gcgagagcca tctgcccgtg attgtggatc ccagccatgc caccgggcac
    16141 tgggacctga ttccgtccat gtcaaaagcc gctgttgccg ccggtgcgga cgggctcatg
    16201 ctggaaatac atccccggcc ggaagaggca ttgtcggacg ggccgcaggc cctgctcccg
    16261 gatcgttata tgaacctgat gaaggaactg ggaacccttg catcggcttt ggggcgttcg
    16321 atctgagcac atgatgcaga atatcaccat tatagggaca ggtctgatgg gcggttcgct
    16381 ggggctcgcg ctgaaagcct ccgggcggcc gtatcatgtc gcggcctgga gccggaaaga
    16441 gtccgaccgc gagaaggcgc ttcagctggg cgtgtgtgat gaggtctttg catccccgga
    16501 agaggctgtt gcgtccgcgg atatttccgt tttctgcacc agtgtcagtt ccattgtccc
    16561 gctgattgaa cagtgcctgg gttatttcaa gaacggatcg ctcgttacag atgtcggaag
    16621 caccaaggcg gacatcgcgc agcggtgcga cgtgatgttc acgggcaggt ctttcagttt
    16681 tattggcagc catcccatgg ccggttcaga gaagagcgga caggaacacg cgactagtga
    16741 tttgtatcgc ggggctgtgg tcgcgctgac tccagtgagc gggccagaac gcgaaatgaa
    16801 ccgtttgaag gaattctggc gcgccgccgg cgcggtgccg gccgtcatgg acccggatga
    16861 acatgaccgc ctcatcgcgc tgaccagtca tctgccgcat ctggccgccg ccgcgctgac
    16921 tgcggcggca tttcagtcgg gtggcgggaa agcagcgggt gttctttgtg ggccgggatt
    16981 cacagactgc acccgtgtcg cgggaagcct gcccgcgctc tggcgggaca ttgcagccag
    17041 caacacggaa aatattctgc ttgcgctgga ggcctatatt gaggagctgt gcggaatccg
    17101 gtcgcttctg gagcagcggg aatatcagtg gcttcaggag tatctcggag agtccgcgcg
    17161 tgcgcgcgag gaactgctct cggctcgcaa aaaaacgacc ggaggcctgt catgaaaagt
    17221 ccgaaggaca gcgtgaccgt ccgagccggc cgtcatttcg gtggaattct tcaagtgccc
    17281 ggcgacaaga gcatttcaca ccgggtggcc atgttttcgg cgctggctga agggctctcg
    17341 acggtggagg gattcctctt cagcgaagac tgcatctgca cctatcgtgc aatggaggcg
    17401 atgggcgctg aggtggagcg ggaggcggac gaccggctga aagtgactgg aaccggggga
    17461 acattccggc cgccgcccgg ggtgctggac atgggaaact cgggcactgg cattcgtctg
    17521 gtggccggtc tcattgcggg cgcggggatt catgccgaga tgacaggtga tgcatccctc
    17581 tgttcccggc cgatgaaccg cattgccgtt ccgctgcgcg agatgggggc gcgaatatca
    17641 ctgcttggag accatggccg cccgcctgtg cgcatcgctc cggccaggct gcgcgccatt
    17701 gattaccacc tcccgatggc ctccgcccag gtgaaatcct gtgttctgct ggccgggctt
    17761 tttgcggagg gaacaacgcg cgtgacggag ccggcccgtt cgcgtgatca taccgaacgc
    17821 atcctgaaca tgctgggtct gccggtccgg gtggacgggc tgtcggttgc gctcgacggc
    17881 tttggtcccg caggaccgcg attcaagggc cggcactgga ttgtgcccgg tgacttttct
    17941 tctgccgctt ttttcatcgt ggcggcggcg ggaatgccgg gcgccgaact gaccgttaaa
    18001 tccgtgggtc tgaatccccg tcgcgcggcg cttctggatg ttctcaagcg gatgggcgcg
    18061 gacattcaga tctgcaattt aaaccagcat caggccggcg agccggtggg ggacgtgatg
    18121 gttcgcggcg cacggctttc gggcaccgac gtgttcgggg acgaagtgcc ggcgctgatt
    18181 gatgaaatcc cgattctttc cgtggcggcg gctgtggccg agggccgcac cgtcattcgc
    18241 gacgcggcag aacttcgcgt gaaggaatct gatcgcattg cggtcatggt gaggaatctc
    18301 cggctgatgg gggtcagggc tgaagaaacg cccgacggaa tgatcgtcga aggcccatca
    18361 caactaattc catcccagcc gcttgagagc ttcggagacc accgcgtggt gatgtccatg
    18421 gcggttctcg cgctttattg caaaagtcct gtttgcattc tgaatacaac ttgtgtaaac
    18481 acatcctatc cgacatattg gaatcatcta cgagagttag gagctgactt tgactgagaa
    18541 aaaacatcac gtaattgcga tagacggtcc gtcggcctcg ggcaagtcca cggtttccaa
    18601 gaagctggct caggtcatgg gctatctcta cgtggattcg ggagcgcttt atcggggcat
    18661 gacctggaag atgctgcagg agaatgtgga cattcatgac acggaagttg tcattgccaa
    18721 gatggacgaa attcagtggg actttttcat caaggatggt tccgtatggt tcagcattga
    18781 cggcacggac cccggacagg aaatcagggg agaggctgtg cgcgaaaatg tcgccattat
    18841 tgccaccatc cccgaagtcc gcgccttcat tggagagcag cttcgcaaca tgcgtcattt
    18901 cgggaacatt gtgatggagg gccgcgatat cggttccgtg gtcttccgtg attcgcccta
    18961 caaattctac atagatgccg atcctcagga acgtgcccgc cgccgctgca aggacattgt
    19021 tcaactggaa gggcagagca atgtggaaga ggttttcaat tccctcaagc gccgtgacga
    19081 aattgattcc agccgtaaaa cagcgcccct tcagattgtg gacggcgccc gcgtgctgga
    19141 tacaaccgga atgaccattg atgaggtggt ggagcgcatt gcttccgccc tcactcagga
    19201 tgggctttcc tgcgaagcct gaaaaaggca ggtttttcaa gttcataggt ccccgctgaa
    19261 ggggtttccc gtgttttttc tggattgcac aagtatttcg ggtccagaaa cttcctcata
    19321 attgagccgc ctgccgcgcg gctgatgatc ttttctgaat cttaactgct tgacatggaa
    19381 aggcttatcg cctataagca caccgttcgg ttctgtatgc cgttcattgg ctcaaaaacc
    19441 tgacataagg cggtacataa tggtacatgt ggtttggggt attatcatag ccagcggaaa
    19501 agaggagcag ctcacttcag gcgtggatgt cgcttttctg aatctcgcgg gaagaccatt
    19561 gctttctcat gctctggagg agtttgagcg ctgtcccgat atcaacggca ttgttgtggt
    19621 cgccccaaaa gaacgagtgg gcgaagtgca ctccatggcc aaactcatgg gatacatgaa
    19681 actgcgcaag attgccgccg gaacggttca gcgtgtctct tcgcttcgca tggccctgaa
    19741 cacccttgat gatgatgttt ccatggtggt cgttcatgaa gtctcccggc cctgcgtgaa
    19801 acgcggactg atttcggaat gcatcaaggc cgcgaagcgc tacggttccg gcgtgtcggc
    19861 atcccgcatt ctggatccgg tgaaaattgt gcccaagggg aacaaagtca cggcgacgct
    19921 ggaagccaac accgcctggg ctgcgcagac cccgcaggcc tataaactgg atgtccttga
    19981 aaaagccctt gccgcggcac agaagaagaa aaagacgctg caggatgaat cggcggctct
    20041 ggaactgatc aagcatgagg ttcatctggt cccttgccct ccgtccaaca tccggatcgc
    20101 gacggccgag gatttaaccc ttgcggaagc cctgaagcgg ttataatcct tccggccgtt
    20161 ttatggcgga cgtgaaggtc accgcgttta caagccagcc gtcatcggtt ttgctccagc
    20221 gcatttccac ctcgcgcaaa tcgctcatcc attcgcctcc gctgcgcgcc ttggcccgtg
    20281 ccaccgtggc cagcaccgcg gagttccggt tcggtgcgat ttcaagttta tgatctgcaa
    20341 tctcgatgga cagctcctcc accatgctcc ggagatgata cgccagcccg gccatatcct
    20401 gacgcgaatg cgagcccgtg agataaggct ccatgccggt ggaaacggga tcggtgaaga
    20461 ggatggaaat ctgccgggcg cgcgtcaggg cggccagctc tttctcgagt gctgtttttg
    20521 aaagaagctc cacaagctcc tcctgccttt gcaggatccg ccgctcatcc cgtccctgca
    20581 ggaaccaggc cagcgaaagc cccagaatca cggtcagcag caggacggcg gaaatctgaa
    20641 aagaacggtt caccacaaaa gccctcccgc aattcggccg cgttcgacag tgcccatcaa
    20701 atgtcttgtg atgggaacgg aacggttgtc gcctgcgaca aaatattcgc tgctcttaag
    20761 gatcacggga gacatggtcc agataccctg atccttcagg taggtttcat catgtgcctc
    20821 cccgtcaatg aagagctgcc cgttctcgaa actgaccgtc tcccccggaa gtccaaggat
    20881 gcgcttgaga tagaaaacgc gccgtccggc cgccctgatg atcaccacct cgccgcgcgc
    20941 cgggctcccg gttctatatt tcagcagatt gacaagccgc acggagcggt ccctcatcgt
    21001 gggctccatg ctggcgccat cgagtcggca gacggtcagc acctgtgtga acaaaatcca
    21061 tgtgatgacc gcgagtccgg ccgcccgcca aagggtggtc gtcagggagc gtcccgcaat
    21121 cagccgtctg aagccggaat tcacggcaga ccccgtatct gctgcagcag ttcacagagt
    21181 cccgccggcc ggctggtctc cagccagatc cgctcataat catccgcgaa atcccgaacc
    21241 tgttccggag tccggcgcac gcgaagccgg agtgcctgga gggccaggcg aatttcgtcc
    21301 agatcctgcc gggtcaccat cccgccgggt cgcagagtcc cggcccggtc cgccagatcg
    21361 tccagctcct cggcattctc tgcattcatg ctcccctgca ggaatcccgg ccaggaattc
    21421 tgcgcagctc ttcccgcgcg gctgaaaagt tccgcggcgc ggcaggcagg cgcgccgaat
    21481 acctgcgcgt caaaggcgcg gtcgaaaccc tctccggaat ccgcctcatt attccatgcc
    21541 agcgtcccgc ccagcgccat gccgtgcagg gaacaggccg gcatgttgaa atgccccatg
    21601 tccccccagt ccgtgaccag catgccttcg gccccgagat gctttgccgc ctgcgcatat
    21661 cccgcgatat tggcgcgcgc cttctcaatc tcgttgaaca gcaccttgaa gcttcttgtt
    21721 gacgggcaca catagaccgc aagtccggca tcccggaaca gctgcgcctt gagaaaatct
    21781 gtttccggct cgtaacccca gtcgagaatg gtgatatctt tcggtatccg gtcaacggcc
    21841 cgcggctgct tcagcagcac atcgccccag cacatcagcc gcttgttgtg ttgcgccgcg
    21901 agcttccgca cgaacgcaag atgttctgtg taaagttcat caaccggtgt tccggcattc
    21961 gcgcctttgc ccaggtcata ggtctcgtcc ccgcacatgt tgaagcggtc ggagctgaaa
    22021 agcggaagaa actcatcaag catccggcca atgagcgcgc gcgatccggc cagccttgga
    22081 ttcagggtgg ccccgcgcag gcgcgtcagc catgccgccg cttcccagct ggcggcaggg
    22141 aactcgattt ccgcaagccg gcgatattcc ggcaggctca gaatgcggcc catgtgtccg
    22201 aagcaggtca ggctgggcac cagctcaatg aatcgctcgc ggcagtactc atccagcgca
    22261 gcaatatctt ctgcggtcag cggatcgcag tgctgcgcga tggcgggatt gaactgaaaa
    22321 tcaaagacat gctcgacata aagctgaagc tgattgtact tcaggaagga gaggcggtcg
    22381 gcgagccgct tgagcgcttc cagtttcggc acccgtccgc ggctgatgtc gagatagaat
    22441 cctcggcggg gcagggaggg ggcgtcctcg atttccattg agggtatggg attgccggcg
    22501 gcgagaatct gccgcagagt ctgcaggccg tagaagaggc cggcccaccc gtcgcccgcc
    22561 accgtgatcc ggcgcaggga tgcggtcagt cgaaagccct ctccgcctcg gccggggggc
    22621 gtgagcagaa caatgccggg tgcatccgcc gccggcgggg cgggtgcttc cgcgattttc
    22681 atcgccagtc cgcgccggtc cagcgcgtcc cggacgggct cgatcgcatg ctgcaggcgc
    22741 gcgcgcgttt tcgggctgca gtgcaggagc acgggagact gaagcatgag ccggccttca
    22801 ccggttttca tccttgccgg gggcggaagc agttccgggt tcaaagagaa attcctgttt
    22861 gtatgtttct cattttcgtt gtaacagtag catggagcag gctgtgaagg aagataaaca
    22921 gtgtatttgc gtgcggtata ccggacatgt gcagggcgtg ggttttcggt acaccgtgat
    22981 gagcattgcc ggccgtttcc ccgtggccgg attcgtccgc aacgagtcgg acggttccgt
    23041 gcggcttgag gccgaaggga ctgcagatga gctgacagca ttcatcaatg ccatccgcca
    23101 gtcttctctg cagcgctata tcatggagga acagatcagc tggcagtccg ccgccggccg
    23161 gttccagggc ttcaccatcg ggcactcccg ctagcggccc ggaaagagat tcgctttcct
    23221 tctagccatc agtcgcttct tttgatagag atttacggaa tgaaatttgc gcttttaggt
    23281 gacatccatt cgaacctgga agctctgcag gccgttttga aggatgcggc agagcagggc
    23341 gtcactgatt acgcctgcat gggggacatc gtcggctaca attccaatcc tcgcgaatgc
    23401 cttgaaatca tccgcaatct gaaatgccgc tgcgtgcggg ggaatcacga tcattactgc
    23461 tcccatgacg agaacctggc cggctttcat ccgctggccg ccgatgtggt ggactggacc
    23521 cgcaagcagc tctccgatga cgagctggaa ttcctgcgca cccgcaagct gatcgagccg
    23581 gtggaatcct tcacgatcgt tcacagcacg ctggacacac cggaaatgtg gggctatgtg
    23641 ttcgacaagt tcgaggccga ggcgaatttc aactatcaga ccacctcgat ctgcttctac
    23701 ggacacacgc acgtcccgct ggcctttgaa aaaaccgaca tcatccgctg tggaatgtac
    23761 acgaaaatcc gtgtcggcct cggaaagaaa tacttcatca atgtcggaag cgtgggccag
    23821 ccgcgcgacg gggatccccg tgcggcgtat gtgatctaca acatggttga caacaccgtt
    23881 gaactgcgcc gggtgcccta cgacttcacc atcacccagg aaaaaatccg ggccgccggc
    23941 ctgccggccc gcctggcttc gcgcctggcc cagggccgtt gaacgaaatg aggacaagcg
    24001 acgcatgaaa tcagcagtaa tcagagttct ggcttttgcg gctttggccg ttcagccggc
    24061 ttttgcgcag atccagttcg gcgggaagtt tgatcacaac cgctatcttc agtacgagcc
    24121 cgtcacagtg aatctgaccg ttgtcaacaa gaccgcgaaa acactgatac ttggcgaggt
    24181 cggcggagaa acgcgcatcc ggtttgacgt tcgcgacacg gccgggcact acatccagcc
    24241 gcggcccggc gcccgcgcga tttccatgct cgtgggcgcg ggacagaccg ttaccaccaa
    24301 cgtgaacgtg cgcgattact gcaggctgga cagtgtcggc agtttcaccg ttacaccgat
    24361 gctggtcacc cccagccgga cctatcttgc gcccaagacc ttccttgaca tcgtttccgg
    24421 cgccgtggtc tcggagatgg ttctggatgt ggacggcggt ttgcgaagct acagcctgcg
    24481 ttcatgcgcg cgtgaccgca ggaccgatct ttttctccgc attgatgacc gggaaaacgg
    24541 aatgtgctac ggagtgcatc tgctgggcaa tgtgctcagt ttccagaagc cgcgcatgga
    24601 ggtggacgag gatgaccgtc tgcacattgt tcaccaggcc catccgtcct atctcactca
    24661 tcacgtgttt tctctgaacg gctcactgat ccgccgcgcc tcctaccgcc gggacggcac
    24721 cgacgatctg ctggatgacg aggatcatat ggaactcatg gcgggggagg ccgtgatgga
    24781 tccgccgccg gccgtgtctg tcccggcgcc cgggcccgcg gcatccgagc cgtcgcttct
    24841 tgaagtctgg gaggcggccg cctccgctcc tgacccggtc cgggcgtcgg aagtatcgga
    24901 agtggacgcc atcattcagt atctggaagc ggacccggtc ccggtcattc cggccccgaa
    24961 ggcgtctgtc cccgccgcgc cgccgccggc cgtttcgatt ccggagccgg ccccggcgcc
    25021 gctcgatccg ctgcaggcgc cagtgccctt ataaatttca acttctggtt gactgatcga
    25081 aaatcggtga tatgtttatg catcaaattg cgtgcacccg tggtgaaatt ggcagacacg
    25141 caaggttcag gtccttgtgc cccttaaagg cgtggaggtt cgaatccttt cgggtgcacc
    25201 attttctttt tccgcttctg acggcagcgg gaggtgcaga cgcatgaaac gcagctggat
    25261 tgaagtggat ctcaacatcc tgcaggaaaa cgtgcaggcc atccgcggcg cgatctctcc
    25321 cggcacggaa ctgatctgcg tggtcaaggc gaatgcctat ggacacggcg cggttccggt
    25381 tgctcagcga gcggcgcagg ccggcgcaaa ctggtttgcc gtcgcctatc tcgaggaagc
    25441 cctcaagctt cgcgccgccc tgccggccgt gaacctgctg gtgctgggcg tggtggatcc
    25501 gtcggacacg gagctcctgc tggagcacaa agtcattcca atcgtcgcca cggaggaaca
    25561 tggcatggcg ctggcgctgg ccgcccgcaa gttcggccgc gacctggaag cgcatatcaa
    25621 aatcgacacc ggcatgggcc ggctcggtct gccgtgggag cgggccccgg acatgatccg
    25681 gcggctggcg aaggagcccg gtttaaacat caccggcatg tgcagtcatt tcgccaaagt
    25741 ggagccggcc gaacccgagg ccgccggcac acaggccgac cggttcttct caatcgcgaa
    25801 agaggccgag gatcttctcg gccgcaggct gttcaagcat ctgtccagca gccgggccat
    25861 tctgtatcag aaggactggg atctgaacgg catccgcccg ggcatcattc tttacggcta
    25921 cggcaccggc aagaaggaca tgcgattcag gaccagaccc atcctgcaat ggaaggcgtt
    25981 tgtcatgcag gtgcgttcgg ttccggcggg gttgcccatt ggatactacg gtacctataa
    26041 aaccaggcag ccgaccgaca ttgccacgct ggccgtcggt tatgccgacg gttatctgcg
    26101 catcctgagc aacaagggtt acgtgctcgt gcacgggcgg cgctgtccgg tggtcggccg
    26161 cgtcagcatg aactggatcg cggtggatgt cggccccgac agcggggtca agcgcggcga
    26221 tgtggccgtg ctgatcggcg agcaggggaa tgagtcggtc tgggccaatg agctggcgca
    26281 gaaatgcatc accatcccgt atgaaatcct gaccggaata gactccagca ttcgccgaca
    26341 gtatatctac tgatagccct gccgccacca gctgccggat gggggagccc gctgaccccc
    26401 gcgaccttgt gtcgcgggtg aggataggtc tgcggctccg gacgcgattg cctgaccttg
    26461 cgctccggcg ggacaagccc gccgggggcg catccgaaaa tggacgggac ggagcccgtc
    26521 cctccaagcc ctaaaatgga gggccggcct ccgtgccggc cgaagagacg gcatgatata
    26581 tcggataccg atatatcaac caaatgtgaa tgaggcgatg ctcatttgcg gcggagccac
    26641 agcatcagac tggccgcgcg atcaggtcat aatccatcgc cgtggtcaca cgcactggaa
    26701 cgaccgttcc gggcatgagt ttcttgtctg acgttccccg caggatgacc agcccgtcgg
    26761 catcgggcga ttggtgtctc gtgtgggcca gcgcgatgca gtcgtgcgga agcggcacat
    26821 ccgggttgcg cacctgccgg tcaatcatca cttcgacggt ttgcccctcc atctcctgaa
    26881 gaaggcggtg tgaaatgtcc atctgctcga tcatcagccg gtcgcggcgc tcgtttttga
    26941 cctcctgcgg gatgtcgtcg ggaagggcgg cggccgcagt gaggggctcg ggtgaatagg
    27001 tgaacgcgcc cagatgattg aactgctgct cccggacaaa ctcaagaagc tcttcaaact
    27061 catcatccgt ctcgccgggg aagccggtga taaaagtcgt gcgaacggcg acgggctcca
    27121 aaagggtgcg gatctgatca agctgtgtct cgacctgcct gcggcttgtt ccccgtccca
    27181 tggctttgag aatccggtcg gaaatgtgct gcaggggaat gtccacatac cggcagaagc
    27241 ggttgtcgcc cgccatgacc tccagcacgt tcttcgacag aaatctcgga taggcgtaca
    27301 tcaggcgcag ccagatgttt tcgggcagtg cggccgccaa atcgcgcaga agacccgcca
    27361 gggccggttt cccaaaaaga tctttcccgt agctggtcgt gtcctgtgcg atgacggcaa
    27421 tttccttcgc gccgttttcc gccagcgaga ccgcctcgga aatgatatcg gccgccggcc
    27481 ggctgatctg ccgcccgcgc atgcggggga tcgagcagaa gctgcatccg ttggagcagc
    27541 cctcgctgat cttcaggctg gccagatgag gcagcccgat cctcagccgc gggccattgc
    27601 ccatgatgtc cgccctcgga tttccatctg cctttctatt gagcagtccg aggcagagcg
    27661 aagcgaggcg cggataatcg gaaaacggca cccgcagatc gcagacggcc agcacatccg
    27721 caaaatccgg ataatccgcg gcgcgctcga ccatgcatcc cagtgcgacc acgaagcgtg
    27781 gccgcccgtt tttctttcgg gccaccagcg cttccagcgt ttcgcgggtt tcagtccgcg
    27841 ccgattcaat gaacccgcag gtgttcacca ggcagacgtc cgcatcgcgc gggtcctcgg
    27901 cgaagacaaa accgtcctgc agcagcgctc ccagcacatg ctccgagtcc acttcgttct
    27961 tcgcgcagcc caggctgacg agactgatca ctggttttga caatttcatc ggcccagcat
    28021 atccggtttg attatccagt gcaatgcgct atccttttct gcatgttcag caagttgcag
    28081 aaagcagagc cggcgaaaag ttccagccat tggaactttt cgcccggaaa gttccaatca
    28141 ttggaagttt ttcgcaggaa agttccaatc attggaagtt ttgtctttct gacgctggcc
    28201 gccgccgctc ccgcgcagga tcagggttcg gcttcggccc tgcaaagtgt ggttcatgaa
    28261 tggatcgccc tgcgggacca gatatcttcc gaggcccgcg agtgggattt ccagaagcag
    28321 tggcatgata ccgagattcg tctgcttgaa tctgaaaaaa agcatctgga agaggatgtc
    28381 cggatgctgc gcggtcagat ggaaagccgt aatgcggcgg atgccggcct gctggccggc
    28441 atagagaagc tgacggcgca gcgcgaggcc tggcggccgc tgctggacga tgccgaagag
    28501 gatattcgct cgtggcagcg cctgctgaaa aaccgcatgg agtttccgct tcccgcgtcg
    28561 ccggaaccgg aggaccgtct gcagcgggtg ttttccattt atgctgaaat cgagaagctt
    28621 cagcaccgcg cttcgacgga caagacggtg atcgagcttg aaggcgcggg ccgccggcag
    28681 atggatgtgc tgtacctggg cctgggagcg gcgttcgccg tggccgccga cggcagcgcg
    28741 gctgcggccg ggaactggac ggagaccgga tggcagtgga cgcgagatga ttcgctggcg
    28801 gaggatgtgc gcagagcggt tcgaatctgc gaggggcagg agacccccgc atgggtgcag
    28861 ctcccgcttg agttgcagga ggccgtgcca tgagaaggct ctgctgcatg ctgatgcttc
    28921 tcgcgctgcc ggcgcatgcc gcgaatgtgg cggaggcgat tgaccggacg caggccgata
    28981 ccgtgaaggc cgtcagggaa ctcgatgaac tgcggtcgaa gatttcctct gaacggcttc
    29041 cgctggccga aaaactttcc cggctggaaa aagatgtggc ccggctgcgc tcggagcgga
    29101 cgcgtctgca gtcggtactc gattcacaca cggagagcgg ggccgggacg gaactgcgga
    29161 aactggaaga acgggcgggg ctgatagagg ctttgttgca ggactaccgc cgctcggtgg
    29221 agggccgcgc ttcggcggcg gagctgcagt atctgaagcc ggatttggat gccgcggatg
    29281 cgtccggcga tcctgttgaa accggccgcg cgcttttgtc gcttgcgaaa cggtggaatc
    29341 gcgagctggc gggcggactg tcgttcagcg gggagtgcct cgacggcgcg ggccgtctgc
    29401 atcagggagc gttcaccgtc atcggccccg tggtctggtt tgccggcagc gggctctccg
    29461 gcctggccgt gaccgcgccc gacagcatgc tcccgtcgct ggctgagcgc gcgcctgcgc
    29521 gttcgggcgt aggattcccc gtggatgtga cgggcggaaa ggttctgaaa atcgagggca
    29581 gccggggctc gtggatggat catttgaagt ccggcggctt tgtcatgttt ccgcttcttc
    29641 ttctcggcct gctggcggtg gtgatcattg tctggaagac ggtcagcctg cggaatatcc
    29701 gtccggccga caccacactg gtccggcaga tcgcggcctc gggagaaagc ggggaactga
    29761 tggagaggct cggtttcccg gcgggcccca tcattgccga aggcctccgg caccgtcatg
    29821 cgccgccgga actgctggag gaaatcatgt acgagcgaat gctgatgcat gttccggcac
    29881 tggagcggca tctcggaacg cttgccgtga ttggcggtgt ggcgccgctg cttggtctgc
    29941 tgggcaccgt gacaggtatg attcacacgt tcaggctggt caccctgttt ggcagcggcg
    30001 aggcccatct gctgtcgggc ggcatatccg aagctttggt caccacagaa accggcctgg
    30061 tgatcgccat cccggtgctg atcatccacg cctattttca tcgccgcgtg aagtccatca
    30121 tcgccggcct tgaactcatg atttcagaat tcgcccgtca gacgggccag gtgaaaggag
    30181 atgcagaatg aaatggatga tgattgcagc tggaatcatg ttttgcgccg cctgcgcggc
    30241 tgaagagccc gtgccgtcca tttatgattt tcaggtggac gacatagaca gcaatccggt
    30301 ttcgatgtcg gaatacaggg gcaaggtgct gctgatcgtg aacgtggcca gcaagtgcgg
    30361 attcacgcgc cagtatgcgg gactggagaa gctctatcag gattaccagg accagggact
    30421 ggtggtgctg ggcttcccgg cgaacagttt catggggcag gagccgggga caaacgagga
    30481 aataaaggac ttctgcacgt ccacctacgg ggtcacgttc ccgatgttct ccaaaatatc
    30541 cgtgaaggga aaggatatgg cgccgctgta ccggtatctg accgacagga agctcaaccc
    30601 gccttccgag gggggcgtca catggaattt caacaagttc ctgatttccc gtgacgggaa
    30661 aatgctgaat cacttccggc cgcggccggg gccggaatcg gaagaactcc gcgccgccgt
    30721 ggaagccgcg ctggaaaccc cctgacggga catgcaggtc ctgaagcata ttctggacta
    30781 ctggaccgcg ggcggcattc tgctggtccc gctggctgcc gtctccttca gcatctggtt
    30841 ttattttctc cggacgcgga gcgcgctcct cgcggcggct gaaattccgt ccggttttga
    30901 gcacaggatt cttgagacgg cggacgagga tcggctgatt cccggcgccg tgaaatatgc
    30961 ggccggcgcg gcgcgggcgg gccgcgaccc gcggcaggcg tggcaggaat atcaggatgc
    31021 ggctgttcgc gggctgagaa aggatgtggt ggtcctcgcg gccctgacgg ccgcggcgcc
    31081 gctcatcgga ctgctcggca ccgtgacagg aatgatcggc acgttccgcg ccgtgatgca
    31141 ggcgcacacg gtgaccggcg gagccgtggc cgacgggatc agccaggcgc tgatcaccac
    31201 gcagttcggt ttgattatcg ccctgccggg ggtttttgga gtggcccgcc tgcaccgcct
    31261 gatccgtcat ctcaacttcc ggttcgcggt ctgccgcagt catctgctcc tcgcgcttgg
    31321 taacggaggg cgttcatgag gcgcgaccgt tattatgatg acgagcaggc cgcgaccgtg
    31381 gccattaacc tttcgccgct gattgacgtc gtttttctgc tcctgatttt ctttgtggtg
    31441 accaccacgt ttgtggagag caccggcatc gaaatagaca agcccgccgc ggtgtccgcc
    31501 gaaaacctcc gcgagcgaag catactgatc gcgctggcgc ccgacggaca gatctatttc
    31561 ggcggacagg ccgttgccct tcagcaggtg cgggggcttg tcgcgcggca gctccagcgg
    31621 ctggaaaggc ccgtgattgt gctggccgac cgccgggcgc agagcggggc gctcatcgcg
    31681 gtgatggatg agtgccggct ggccggcgca aagaaggtca atgtggcaac ggagaagcat
    31741 gagtgaactt cgccaggctg tgatgcggca ggcgccggct cccggaacgg tgctgctccc
    31801 gcttttgctg acgacggccg tttttcttgc cctgcctctc atggagcgga tcacggagcg
    31861 cgccgccgat tatgtgatcc ggccgctgga tatgattgaa cagatgccgg ccattccgga
    31921 gccccagccg gttcgtactg ccgtccagag ccggccggtt cgcatggaac agcggccgga
    31981 actgctgcgg ccttccgatg tgcagatgcc ttccatgccg ctttcggaaa tggcggacat
    32041 ggtgcggctg gatgcgcttc cggttgatcc ggccatggcg gtgttcgaca tcgcgctcgc
    32101 ggcgggcggc ccggtggatc tcagtgcgct ggatgtgatt ccgatacctg tcagccgcat
    32161 ggatccggtc tatccggcat cggcccgggc gcgcggcatc gagggttttg tgacgctcga
    32221 attcgtgatc ggagaagacg ggcgggtgag cagcgcgcgg gtgacggatt ctgttccggg
    32281 cggaatcttt gacgatgcct cggttcgggc cgttggatca tggcgttttc ggccggggga
    32341 aatggatggc cgccccgtgg ccgtaaaagt gcgtcagaca ctcaatttta agctgaagga
    32401 gtaaccgtgc cgaaaaacgc cgaaaatgct caaaaatggg ccaaaaacgg ccaaaaatgc
    32461 tcaaaaatgg gccaaaaacg gccaaaaatg ctcaaaaacg gccatttttt gccaaaaatc
    32521 ggcatttttt cgtttttttg gatgtttatg gtttacgcgg atgccagtca gcccgttatc
    32581 tcctctgaaa cggctgagac gcttaaccgg gtccagtctg ttgctgaaac gaatctggca
    32641 atggccataa cacagatgga gaattacagc cgcgatgatg ccggcgccgc cgtgatcttc
    32701 acgctcggga atttatattt gcgggacgga aaactcgacg aggccaaagc cgccttttcg
    32761 caggcgctgg aaaagcattc cgattttgag caggcccggc agaatttggg tcacgtggct
    32821 tttttgcagg agcagtatga cgacgccatc ggcatatttc aaaaactgat cagcgacggg
    32881 aaagccaatg cggaaatctg gctgatgctg ggtcatgcgc tggtgctgac ggatcatccg
    32941 gtggcggccg agaatgccta tcgcaacgcg ctgaccctgc agcttgaaaa tcctgccgca
    33001 aaacaggggc tgcttcgcgc gctgattgcg caggagcgat acccggaagc ctcaaaactg
    33061 ctggacgaac tgatgctgaa aaatccggaa gccggagaac tgtggactct gaaggcgaat
    33121 cttgcgctgg cccttggcaa agacaccgaa gctttgacgg cgctggaatc agcccggcgg
    33181 ctgggcgtga ccgaaatatc cgtgaaactg cttctg
//
LOCUS       DAOQWR010000010        32095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1634_length_32095_cov_6.195568, whole genome shotgun sequence.
ACCESSION   DAOQWR010000010 DAOQWR010000000
VERSION     DAOQWR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 32095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32095
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_32095_cov_6.195568"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKK36_01695"
     CDS             <1..180
                     /locus_tag="PKK36_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93302.1"
                     /translation="AGAWIETPAMTDGSPQWAVAPARQSKIFQSPEVFAGKVPIIGNF
                     HFSCNKTPLSGTLRA"
     gene            177..824
                     /locus_tag="PKK36_01700"
     CDS             177..824
                     /locus_tag="PKK36_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93303.1"
                     /translation="MNTASKRNVHALILCVYQNEGEMFLESICPDCAGGPCALKVDNT
                     NVRLDLLAGDPALNASWLSRIQEADALILLVRFMDVTSIDKLNAIHRSIPDEGSPPLF
                     VFLFREQDQAEFKRSCPTCGQKLWVRDMDVGREGRCPNCKASFALLSQEDLIKKEIVL
                     PDSVPVIRIYQGDEGSVKSAFAWILPGVTSRLAEANPFDEETLKKSTIRVQIDDL"
     gene            complement(834..1079)
                     /locus_tag="PKK36_01705"
     CDS             complement(834..1079)
                     /locus_tag="PKK36_01705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNR93304.1"
                     /translation="MKAEKKNEDPGFEKALERLEKIVTEMEEGDLSLDKMMQHFEEGS
                     RLVQYCSARLNEVEKKIEQLVKKDGGTGTAPFEAEAE"
     gene            complement(1089..2363)
                     /gene="xseA"
                     /locus_tag="PKK36_01710"
     CDS             complement(1089..2363)
                     /gene="xseA"
                     /locus_tag="PKK36_01710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNR93305.1"
                     /translation="MNAPAKPEVFSVTSITRRIRFLLEEEIGSVWVEGEISNFTRAAS
                     GHLYFSLKDEGAQIKAAFFRGSQTGLNIEPANGMQVRVQGEVTLYEKTGSCQIIVRRM
                     ESAGAGSLQEAFEKLKARLEAEGLFAPDRKRSLPMLPQHIGIVTSPTGAAIRDMLNVL
                     GRRFPNLHIVIAPVRVQGEGSAAEIAGAIGDLNRLGYIDVIIAGRGGGSIEDLWAFNE
                     EVVARAIAASAVPVISAVGHETDFTIADFVADLRAPTPSAAAELVIDAKENFENLLAQ
                     RRRQLARALENRFLKLKQRLVRAEHSYVFREPGNLVRNYVLRVEHLGQSLRQRLLRAA
                     QVRHQQADSLNMRLGHLAQSVFQSHRQRVRQLDAQLRALNPHAVLDRGYSVTRCADGT
                     IVKDFKQVKTGDALATQLARGRVESTVTGTQL"
     gene            complement(2360..3136)
                     /locus_tag="PKK36_01715"
     CDS             complement(2360..3136)
                     /locus_tag="PKK36_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNR93306.1"
                     /translation="MKILMTGDIVGQPGRTAFARTAGRMREQQGVDMIIANAENSAGG
                     RGITPVICDELFAAGADVLTLGDHTWDQKELAPWLKNAKRVVRPANFAPGCPGTGCVT
                     VMVKNIPVTVISLVGRVFMSPYDCPFRTADQILAQKNPPGGIIMVDVHAEATSEKIAL
                     GRYLDGRVSAVAGTHTHVQTADEEILPGGTAYITDLGMTGPKDSILGRDVNSVLQKFL
                     TGMPSRFSIATEKVAFEGALVTVDDKTCRATGIERVREYM"
     gene            complement(3154..4737)
                     /gene="rny"
                     /locus_tag="PKK36_01720"
     CDS             complement(3154..4737)
                     /gene="rny"
                     /locus_tag="PKK36_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNR93307.1"
                     /translation="MRWDQIDVPWWLPTWTAFVGILMGLMAGYLLINLLERRRAGRAQ
                     LDSTRILSDARKDAEHILKEARLKAQDMQIQAQQDFERETREQRDELRMLEARIMRRA
                     ANLDRKLSMLEKKEEKLEQQQEELNDRKSQMEARQLELEQLISTKKAELGKIASLSQE
                     EARAQLTRILDEEIRADAGVLIRHIQDEAKETAEKKAREIIAGAIQRYAASQVSDMST
                     SSVPLPNEEMKGRIIGREGRNIRALEAATGVDILIDDTPEVVVVSGFDPLRREIARVT
                     LERLIADGRIHPSRIEDTARKVTEEVEEIVRESGEAAVFELGLQGVDPELIHTLGRLK
                     YRYSFAQNVLQHSIEMAHLMAMMAAELGLDVNIAKRVGLFHDIGKALDHKVEGGHATI
                     GADLLRKYGEQPVVVNAVGAHHGDIEPESIYATLVEAADAMSAGRPGARAETTQIYLK
                     RLTDLENIASSFRGVEKSYAIQAGREIRVIVEPSKIDDNQALQMARNITKQIEHDLQY
                     PGQIKVTVIRETRCVEYAK"
     gene            complement(4734..5297)
                     /locus_tag="PKK36_01725"
     CDS             complement(4734..5297)
                     /locus_tag="PKK36_01725"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNR93308.1"
                     /translation="MREIIAARRRNLDQAWIWSASRAVAGSAAALSEFAAAGTVACYL
                     ALPAEVQTEPLISLCRQAGRNVCVPALDPQTGRYSFARLRTDEKLVVGEDRTEQPCTP
                     CWTDPREIDLMIVPGIAFDAAGGRLGRGGGCYDALMAAFRGPRIGLAFECQMLERVPR
                     EAHDETVNIIVTEERILKIQPDGGNKQ"
     gene            complement(5312..6667)
                     /locus_tag="PKK36_01730"
     CDS             complement(5312..6667)
                     /locus_tag="PKK36_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNR93309.1"
                     /translation="MDLFADNQNPGGEQRAPEDKTGGEPLAARMRPQTLEDVAGQEHL
                     LAPGKLLRRAIEADRLGSIILYGPPGCGKTSLAEVIAKVTQRNFVRTSGVLANVATLR
                     TLLQAARQTRQAYGRETILFIDEIHRFNKAQQDVLLPYVEDGSITLVGATTHNPFFFI
                     NTPLTSRSQIFELEALSEKAVVTLLRRALERDREMTARNASADDDALEHLARVCEGDA
                     RRALNALEIAVLTTPPDAAGRVRVTRAAAEESIQKKAVVYDHDEDGHYDTISAFIKSV
                     RGSDPNAAVYWLAKMLYAGEDPRFIARRLIILASEDIGNADPRAVTLAVSVLNAVEFV
                     GMPEARIALAQCTTYLATAPKSNAAYMAGEAAMKDVKEGRTLPVPRPLRGATYKGAEQ
                     LGHTGYKYAHDYEEGFVDQEYAPTTKIYYEPTNRGYEETIRKRIEHWNSLRSKSRKRQ
                     S"
     gene            complement(6693..6873)
                     /gene="ssrS"
                     /locus_tag="PKK36_01735"
     ncRNA           complement(6693..6873)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKK36_01735"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(7184..9592)
                     /gene="pheT"
                     /locus_tag="PKK36_01740"
     CDS             complement(7184..9592)
                     /gene="pheT"
                     /locus_tag="PKK36_01740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNR93310.1"
                     /translation="MKLPLCWLKDYVDFDDTPQGLADRLTFSGVEVEGIETIGGDFPG
                     VAAGEVRAVNPHPNADRLKLVTVFDGSREHVVVCGAPVVEVGAKYPFAVTGTTLPGGM
                     TLKKSKIRGVVSEGMLCAEDELGISDDHSGLMRLDDSCKPGTPLSEILGPPETVLDLE
                     ITPNRPDCLSIIGLAREVSALYGRPLRLPPADFEETSEDVNALAAVEVKEPEKCPRYT
                     ARVLQNIRIAPSPEWMQKRLKLAGIRPISNVVDITNFVMLEAGQPLHAFDKSLLADSR
                     IIVRLPSPDDRLSTLDGIERKLEPDMLLIADAKRPVALAGVMGGAGSEISDNTTSVLL
                     ESACFGMDTTRKTSRRLGLSTDSSYRFERGVDIGGVDWASRRAAKLMIDLAGAQCARG
                     VIDIYPKPPAARSIVCRFDAISSLLGIEVEPGRVWQIFEGLGLAVTAVDEASCAVGIP
                     TFRGDLEREVDLIEEVARIYGLDKIPSPAPQTRVVPGADDRKTRARFACRGTLVGLGL
                     REVMSYSLVAPQLLDLFDPAGAKSRIVLPHPISTDQSVLRTSLIPQMVENLGYNRARQ
                     LPEAAFFEIGHVFFHDAKGAPSEEERVAIGLMGPVGRCSLDKRKPVTEGEAFLWIRGV
                     VEALLSDLRMTGVSFVRTDRPYFEPGHAAAVAVAGIEIGVLGVVRRAVRDEWRISDPA
                     AVAEIRTADILGGVFTLKRIRELAPYPPIQRDIAMLVDEAVTNEAVMDIIRGAAPREL
                     ERIELFDVFTGKNTGPGRKSMAYSLIFRSAEKTLTDEQANGYYDVVKRALEKALPGIE
                     IREG"
     gene            complement(9669..10721)
                     /locus_tag="PKK36_01745"
     CDS             complement(9669..10721)
                     /locus_tag="PKK36_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93311.1"
                     /translation="MKKIDWNRNVLQGLSEADYLMGRLGGTMDRMPEPEIRHLLLIRE
                     VSALCRKDWVAVLKSHAESARYGSSVESAMNAFHQAAGESPGFSSVSVRRFVDLHVVL
                     HQDQVSERRASSRFESAAEHLRGIDPFLIRAGLMLCESTLLENELPGAQGTGQLVFTA
                     LTVSGAMVPWPGLGISPFLNATSADHLKLCRAVQKTGDPEPWLLYFLDGVARQCEDLI
                     GRMARIEETVQTWKRTLSAPRHAAALLLTEKLAGNPFLTVRGAAAQLDAAFTTAQRAV
                     DKLLEAGILQETTNQRRDRIFCANDIFAVLNEPPRVISAAPWREKEIIPELKLLEEPP
                     APPPRHDPAAKLPIPY"
     gene            complement(10972..11766)
                     /locus_tag="PKK36_01750"
     CDS             complement(10972..11766)
                     /locus_tag="PKK36_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR93312.1"
                     /translation="MKLKSWTAAAILVLAAWMLCASDGAAQESQARTVFDHILYLTIE
                     SEPSGAAVYALTDGGKKVGDMIGVTPCRTMVGLKWDRTAGFRRWTRLSIWAPGDVCYA
                     LLDTDKTWDIFASCSAVLPGCRPKIVNERILSLPSPGLDWSEAENWPSDLTLKLNLTQ
                     VAREPLDETRSASQAPASVIMASGSAADPSTVGTINITASTDGATAYVNSKSAGRVPV
                     SVKLPAGTYTIAVFKDGWTPFNDRVEIKSSSVTAIHAEMKPRAPAP"
     gene            11962..12243
                     /locus_tag="PKK36_01755"
     CDS             11962..12243
                     /locus_tag="PKK36_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR93313.1"
                     /translation="MAKAKTKSQIIAGIAEDAGLTKKQAAAALDSLVSQAYKGAKDGF
                     TIPGLGKLVKVKRKARMGRNPATGEAIKIAAKTVLKFRIAKAAKDAVLG"
     gene            12387..13466
                     /gene="serC"
                     /locus_tag="PKK36_01760"
     CDS             12387..13466
                     /gene="serC"
                     /locus_tag="PKK36_01760"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNR93314.1"
                     /translation="MARVYNFSAGPATLPLEVLQEAQAEFVDFKGTGMSLIEASHRGK
                     AYDAVHEEAVANISKLAGLTDDYAVAFMTGGASQQFAQIPMNLLGENDTADYVNTGAW
                     TKKAIAEAKLQRSIKVIADVSKESPLRMPREDEFKVNKDAVYLHLCSNETIAGTQLKK
                     FPKVDVPIVADMSSDMFSRPFDIRQFGAIYAGAQKNMGPAGVTIVIMRKDLAARVSEK
                     VPKIFRYTTHIENNSLYNTPPTFSIYIVMLVTRWIMKNGGLAGIEKMNAAKAAKIYDA
                     IDASSFYRSPVANDSRSFMNITFRLPSEELEAQFVAEAKKLKMDGLKGHRDVGGIRAS
                     IYNAFPMEGIDALVQFMTDFEKKNG"
     gene            13478..13552
                     /locus_tag="PKK36_01765"
     tRNA            13478..13552
                     /locus_tag="PKK36_01765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13511..13513,aa:Arg,seq:ccg)
     gene            13627..14382
                     /gene="rph"
                     /locus_tag="PKK36_01770"
     CDS             13627..14382
                     /gene="rph"
                     /locus_tag="PKK36_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNR93315.1"
                     /translation="MPTKKKNDHRRADGRKLDELRKICITPGVAPAATASVLMEAGRN
                     KIICAVSIEEDVPRWMRAQKITGGWVTAEYSMLPYASEERKRRDITAGKIDGRSQEIQ
                     RLIGRSMRAVIDLEQLGPRTVWIDCDVVRADGGTRTASITGAFVACRLAVNRLIAEGR
                     LAVNPIREALAAVSVGIVGGQPMLDLAYTEDVAAETDMNVVMTESGKFVELQGTAEEH
                     PFSKHELSALLKLAEKGVAELLKKQRDAIRSAK"
     gene            complement(14389..15579)
                     /locus_tag="PKK36_01775"
     CDS             complement(14389..15579)
                     /locus_tag="PKK36_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93316.1"
                     /translation="MSEENKTPPSAQPNSKPAGAPAPKPALKKPALKKPAAAGSSPAG
                     PTIKIGARPVAKTPRIDLADKTAEEAAQPAPAKPAGEADLGTLKQAIQKTSAQIESAR
                     KDLQKPPAPVSDIREKEVPPAPGKKATMFMDVDAIASAEPAAKKATMFMDVDSAEAEK
                     PQPPPPVDAKKKTSRISTASAVPPAPQETLDEFTPAPSASLAKNATMFMNIEDDLGEA
                     PQTQPKKQTARIDISSAIPSPRDTTKISAGAPATIRIKRPVTTSTARKPPARPPEPMV
                     PHSVLGQQPGSAMMAEGKKSETSRIELPPDAVRPPTRKKTVKIKRPDGSDAGVPKLSL
                     QRPVTSSFQAAQVEIPAKPEKEEAGIAFSIVSIAATIVLCVLVYVFLAQVLANGLPFP
                     SPIN"
     gene            15709..16242
                     /locus_tag="PKK36_01780"
     CDS             15709..16242
                     /locus_tag="PKK36_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93317.1"
                     /translation="MLKMPYIAGILIALVAAASLLTGCKPSGGGEEEMMPQPIGFDLP
                     GAGSAPVHDVRAMSDLALQIVREAPAEEGGGATTGGQIEEQTAALEGSVMFPRWTARR
                     VGANDYEVRFTFTHIDNQYNMNKTGFSWIVNVAQKTAGPRRVLRPEDFDEAGGAMRTP
                     CDWEPLMFRESSAHEKQ"
     gene            16229..17329
                     /gene="mutY"
                     /locus_tag="PKK36_01785"
     CDS             16229..17329
                     /gene="mutY"
                     /locus_tag="PKK36_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNR93318.1"
                     /translation="MKNSSREQVLKTAFEQHLPEWFALVRRAMPWRVNRTPYRVWVAE
                     IMLQQTRADQAAPYYRRFVRTFPSVRALARASLRDVLKAWEGMGYYARARNMHKTAQH
                     LMNTRGGRFPRTYEELKQLPGIGSYTAAAIGSLAMNLDVAVVDGNVIRVLSRVFGVDE
                     PVDTAAGRRIIQGFAESLLPAGRAAEFNEAMMELGALCCTPRDPRCEKCPLSAVCRAC
                     GLGRSTEYPRKRRRKAVPHKVVGAGVIMDRRGRVLIAQRRTQDMLGGLWEFPGGKKEP
                     EETMPECITRELREELGIEVKVGELVTVVHHAYSHFTMELHAHRGRVVKGRPRAIECA
                     DCAWVLPDELREYPMGRADQHIVAALLQEDAG"
     gene            complement(17334..18215)
                     /locus_tag="PKK36_01790"
     CDS             complement(17334..18215)
                     /locus_tag="PKK36_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR93319.1"
                     /translation="MTDIKEYLQRGKALVETALDRNLPREDDPPARLHQAMRVSMLAG
                     GKRLRPILCLAAAEAVNPGADALLPALSLEILHTYSLIHDDLPAMDDDDLRRGRPTCH
                     VEFGEATAILAGDALLTLAFELMGRCAAPPPWMPLQLVTELAQAAGSRGMIRGQMEDM
                     QGENRSLGAEEVRSIHLHKTAALIRAAVRMGAIAAGAPPEVVDSFSVYGEKSGLAFQI
                     MDDVLNETSTPEELGKPVKSDRDRGKSTWCGIYGVRESQRQAAELIDEAVAAVRETGL
                     QTATLEDIARFLIQRRS"
     gene            complement(18274..20004)
                     /locus_tag="PKK36_01795"
     CDS             complement(18274..20004)
                     /locus_tag="PKK36_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNR93320.1"
                     /translation="MHYLDESHILLFLIQIFILMGCARGAGELLRRWGQPALTGEILV
                     GIFLGPTILGRLWPELQLALFPPDAIQQTMLDTVAWVGVLFLLLDTGLEIDFSIAWRQ
                     RGSAFLIAISDIIIPVAVGFAAVYFLPDRFLVNPEQRIIFALFMATVMTISAMPVAAR
                     VLHDLKLLKADLGFLVMSALAVNDIVGWVLFTIILGTFTTSTAAVGPIFMVFTSTVGF
                     AALALTLGRPVSTRVFDAVKRGKFPEPATSLTITVLLGLLFGVVTSWLGIHALFGFFI
                     AGVVVGEAKSLSEQTRGVISQMVHSLFVPLFFANIGLKMDYIADFNLPVVALICAAGI
                     GGRYAGAWFGVTWARVSRINRDLISIAHTPGGMMEIVVALLALEADLITPAIFVAIVV
                     SAVFSSMLMGPWLARSLDRRAAVTPARFLQPDNIVADLAGASRAEAVQELAERIERQA
                     GSGTRDILVQMAMRREDEFGTAIGEGVAIPHARIDNLKNPVIAYGRSRQGIDWDAPDG
                     VPVEDVFFFATPMGTEDIHVQTLSAIASSMASADMRRRLHAASGPEELYAILQSLLVG
                     RKPHNRLVRT"
     gene            20161..21282
                     /locus_tag="PKK36_01800"
     CDS             20161..21282
                     /locus_tag="PKK36_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93321.1"
                     /translation="MKGKLIILSTLILLAVLPLAGGLLKGLPEDFFSFPPLVEHSPVH
                     APFSPPVFSMFAALCVLTALFFLFPFYFGFARQTASPPSAGGRFPVWGRLGLALMLFS
                     WICAWGQFDFLGRARLHTFFPLWVGFIFTMDGLVFRRTGRSLFSGRRAAFFTLFPVSA
                     VSWWYFELLNRIVQNWWYPDRTDFGPAHYLIYSSLCFSTVMPAIFEIREWLMSFPPMR
                     VRYARGPSIRLAPLPLALASAVLLALIPLFPDPLFFVVWIAPLGLLGSALALARVKSP
                     FSAMTEGDWTVAVNLGLAALICGFFWELWNFYSTPCWEYSVPYVTRAHLFAMPVVGYA
                     GYLPFGPVCACFWLAWTALLPGKIRIRRAGPPSLAPGAP"
     gene            complement(21239..24919)
                     /locus_tag="PKK36_01805"
     CDS             complement(21239..24919)
                     /locus_tag="PKK36_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR93322.1"
                     /translation="MPINQEQTREQRSAVGRLIRFCLENRLVVSLFTFAVIFAGILVA
                     PFDWKAGGLQRYPVAVDAIPDIGENQQIVFTEWTGRSPQDVEDQIGYPLTVELLGIPG
                     VKTIRSYSMFGFSSIYIIFKENVEFYWSRARVLEKLNSLSSGTLPDGVQPKLGPDATA
                     LGQVYWYTLEGLDPDGNPTGGWDLEELRTVQDWYARYWLLSADGVAEVASIGGYVREY
                     QVDVDPDAMRAYGVSIDEVYRAVQGANIDVGAKSIEINRVEYFIRGVGFIKTLADIEN
                     SVIRVGADNVPVLVKHVAQVQLGPAARRGALDKGGAPAVGGVVVARYGDNPLKVISSV
                     KARIAETRDALPKKTLADGTVSQVTVVPFYDRTGLIYETLGTLNKAIGQEILITIIVV
                     LIMVMHLRSGLVISSMLPLAVLLCFIAMKLFNVQANIVALSGIVIAIGTIVDMGIVIC
                     ENILRHLDEAGPSESGLEVVHRAAAEVGGAVLTAVLTTVVSFLPVFTMIGAEGKLFKP
                     LAFTKTFALIASIIIALTILPVLAHTFFGRTRTPGTPSHPPRRFFPALLKKYAPITLI
                     VLLVGTWLTFEWEPLGPERGVIRNLIFVVLLIGGLLGFFRVFQHFYPQMLAWSLRHKK
                     TFLAIPLVLLVFGATVWLGFEKLFGWLPDAVRKTVPAQKIAHIFPGFGKEFMPSLDEG
                     SFLWMPTTMTHASIGEALDILEKQDIAIRSIPEIESVVGKLGRAETPLDPAPISMIET
                     VINYKSEYREDKDGRRISYKYKNGEFVRDAAGELIEDSRGRPYRQWRDEIRSPDDIWN
                     EIVRAAKITGTTSAPKLQPIAARIVMLQSGMRAPMGVKVQGPDLGTIEAVGLQIEKFL
                     KEVPSVEPSAVIADRIVGKPYLEIVPDRESLARYGIPIRKFQDVVEIAIGGRKVTGTV
                     EGRERFPVRVRYSRELRDSIEALDKILIPGTGGQQIPITELAEIKYVRGPQNIKSEDT
                     FRVGYVVFDKKPGTAEVDVVEQCQAHLQSKIASGEFSLPPGVSYRFAGSYENQLRASR
                     TLALVLPVALFFIFMLIYLQFKSVSTSLLIFSGVFVAWSGGFILIWLYAQPWFLNFSL
                     FGVEMRSLFQVHVINLSVAVWVGFLALFGIATDDGVVMGTYLTQIFRDKPHTTTDEIR
                     ALVIEAGTRRVRACLMTTATTLLALLPVLTSTGRGSEIMVPMAIPSFGGMLIEVITML
                     VVPVLYCGIMERRARERAGPPA"
     gene            complement(24909..25079)
                     /locus_tag="PKK36_01810"
     CDS             complement(24909..25079)
                     /locus_tag="PKK36_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNR93323.1"
                     /translation="MGGKINKDFFADYNGMRIYFCCGGCDETFRKNPEKYLQEMREKN
                     IAPERLEDLHAH"
     gene            complement(25143..26444)
                     /locus_tag="PKK36_01815"
                     /pseudo
     CDS             complement(25143..26444)
                     /locus_tag="PKK36_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672963.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(26580..27830)
                     /locus_tag="PKK36_01820"
     CDS             complement(26580..27830)
                     /locus_tag="PKK36_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR93324.1"
                     /translation="MKFNRLLLAAAAMLTACAAGAKADDLRDYLAAAARNNPELEAAF
                     NRWKAELERIPQVRALPDPRFNYAYFVEEVETRVGPQKQRLGLSQVFPWFGTLKLRGE
                     AAAEAAEAERQRYEQAKRQLFYRVKSAFFEYHYLNRSIEITRQHLALLQQIEQVARTR
                     LQTGEIQQNPVIQAQVELGKLDDKLRTLEALREPLSARLSAALNRREDPVLPWPDSPV
                     VTPAAFTDDEVRQWFSEASPEIMRLEAVIRKEQKSAELARKARYPDIMLGLDYTQTDE
                     ALMPGVSDSGKDPIMATISVGLPLWFGKLKAAEQEAHFRRAAAEALRTAAENTLSADL
                     QMALFNFRDAERKMNLYSDTLVPKAMQALEVTRESFETGRDSFTALIDAERMLLEFQL
                     AADRAFTDREIRLAEIEMLINRDL"
     assembly_gap    27996..28095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28126..28908)
                     /locus_tag="PKK36_01825"
     CDS             complement(28126..28908)
                     /locus_tag="PKK36_01825"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HNR93325.1"
                     /translation="MKKYSVFQAPLYAFFSKDFYADIGRNHRGTAFGYLFLLLLVCWV
                     PVVMTWHGPFAEAVDSYAPGLIERIPEMTITDGRAVIHADQPCFIPMPHSTNTLAIFD
                     TTGKYTSLEGQDAFVLVTETKVIMKQSAVETRSYSFAEIKNFALTREKMTNWVALGRK
                     WIMPSVYAAVLISSFIGKVIKMLIYALIGLLFAKSAVPYASVLRLSVMAGTPAIVLHT
                     VFMLTPIGFFIPGIAWFVLTLVYLYIGVRATTAAETPAPTSV"
     gene            29022..30011
                     /gene="rnhC"
                     /locus_tag="PKK36_01830"
     CDS             29022..30011
                     /gene="rnhC"
                     /locus_tag="PKK36_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNR93326.1"
                     /translation="MKKNSYTFKLTPEQQRELEQALRTGNYRPKQVPHTRIAVEGPDY
                     NVALYTSGKCLVQGKGAEEWVTFTLEPYILREVVTGYDKILHPEAIEAHMGIDESGKG
                     DFFGPLVIAAVYTDEAIADAFREMDVRDSKTITSDAKAEKMAEAIRKILKGKFAIVKI
                     GPEAYNRLYSSIKNVNRLLAWGHARAIENLLDKVPGCPKAIADQFGPKQTIEKALLKK
                     GRKITLVQRHKAEADPAVAAASILARDGFLQGLKALGEKYKVKIPKGASDQVRAVARD
                     LVQKHGPEVLVQACKCHFKTADAVLAEMNMNRAALGPLGQAVSKDYIRKTKEG"
     gene            complement(30045..30323)
                     /locus_tag="PKK36_01835"
     CDS             complement(30045..30323)
                     /locus_tag="PKK36_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR93327.1"
                     /translation="MAMTKSQIQAALAEKTGLSKKDVAQVLDELGALAIKEAKNGFAL
                     LGLGKLVLVDRAARMGRNPATGETIQIPAKKVVKFRVSSACKQALLKG"
     gene            complement(30443..31573)
                     /locus_tag="PKK36_01840"
     CDS             complement(30443..31573)
                     /locus_tag="PKK36_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93328.1"
                     /translation="MKVVRQKSPDSRYRELLGGLYEAVFICRSDGRILDTNVRAEEHF
                     HYSRDELLAMNVCDVFPGLSQTKIEKILGYLKDRRFTVLNGKCVRKDGTIFPGETSVS
                     RIQMADEEALVFSITNIERRVKTEEMLRTEHNAIQNLASGVAITGPEGLLQYVNPTFL
                     RLWGQRSENEVLKKNIRDFIPAGPKTDEMIESVLSGATRQTEMEVPRPGGKPFFVQVS
                     IAPNIPQSPIFGSGTKVMGMVFSFIDITDRKRAEDVIYKEAQAQMKRVRDENSKFSGL
                     LNILSIPDLIQLISSTQKSGGMVLTDDSNRRVADIAFANGQIVAARCESLTGEDAVLQ
                     LGRCGGVEFSFEPGEPKFRDASITRPTMALLLEMMRLMDEQA"
     gene            complement(31575..>32095)
                     /locus_tag="PKK36_01845"
     CDS             complement(31575..>32095)
                     /locus_tag="PKK36_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93329.1"
                     /translation="MRMKKHTGAAMLAGALLLPCAQAARLSEQTNFTFTAESLNAGGI
                     LMATNVSGTVRLSGSIAQHGFTSLSTNVEGRVLQSGFWWADEVREVEPAQFTGLSLGA
                     DQIGITFSVVSGNWYQVLYVTDSQGGLSAGRHSFTNIASALFEATGISTTVWQSVGSP
                     PDTARYYLIKCQ"
BASE COUNT         6382 a         9761 c         9329 g         6523 t
ORIGIN      
        1 gcgggggcgt ggattgaaac gccagcaatg acggatggat caccacaatg ggccgtcgcc
       61 cccgcgcggc aatcgaaaat tttccaatcc ccggaagttt ttgcgggaaa agttccaatc
      121 attggaaatt ttcatttttc ttgtaacaaa acgccgcttt cgggtacatt gagggcatga
      181 acacggcaag caagcgcaat gtgcatgccc ttatcctgtg tgtgtatcag aatgaggggg
      241 aaatgtttct cgaaagcatc tgcccggact gcgccggcgg cccctgcgca ctgaaggtgg
      301 acaacacgaa tgtgcgcctg gacctgctgg ccggggaccc ggccctcaac gcctcctggc
      361 tttcacgaat ccaggaggcc gatgcgctga tcctgctggt gcgcttcatg gatgtgacct
      421 ccattgacaa actcaacgcg atccaccgca gcattccgga tgagggcagt ccgccgctct
      481 tcgttttcct tttccgcgaa caggaccagg cggagttcaa gcgcagctgc cccacctgcg
      541 gccagaaact ctgggtgcgc gacatggatg tcggccgcga aggccgctgc ccgaactgca
      601 aggcctcgtt tgcgctcctg tcgcaggagg acctgatcaa gaaggaaatc gtgctgccgg
      661 attcagtgcc ggtgatccgc atttatcagg gcgacgaggg ctccgtgaag agcgcctttg
      721 catggattct gcccggagtc accagccggc tcgccgaggc gaatcctttc gatgaggaaa
      781 cgctcaagaa atccacaatc cgcgtgcaga ttgacgatct ttagaacgcc ggactattcg
      841 gcttccgcct caaacggcgc ggtacctgtt cccccgtcct ttttcacgag ctgctcgatc
      901 ttcttctcca cctcgttgag ccttgcggag cagtactgaa ccagccggct gccttcttcg
      961 aagtgctgca tcatcttgtc gaggctgaga tcgccctctt ccatttcggt cacgattttt
     1021 tcaagccgct cgagcgcctt ttcaaaaccc ggatcttcgt ttttcttttc cgctttcatg
     1081 ttcactcctc agagctgtgt tccggtgacg gtggattcca cccgtccgcg cgcgagctgc
     1141 gtggccagcg cgtcgccggt tttcacctgt ttaaaatctt tcacaatggt cccgtcggcg
     1201 cagcgcgtga cgctgtagcc ccgatccagc accgcgtgcg gattcagcgc ccggagctgc
     1261 gcatcaagct gccgcacccg ctggcggtgc gactgaaaaa ccgactgcgc cagatggccc
     1321 agccgcatgt tcagggagtc cgcctgctgg tggcgaacct gcgcggcgcg cagcagccgc
     1381 tggcggagcg actggcccag atgctcaacg cgaagcacgt agttccgcac caggtttccc
     1441 ggttcgcgaa acacataact gtgctccgcc cgcaccagcc tctgcttcag tttcagaaac
     1501 cggttctcca gcgcgcgcgc aagctgcctc cggcgctgcg cgagaagatt ttcaaaattc
     1561 tccttcgcgt caatcaccag ctccgccgcg gcggacggcg tcggcgcgcg cagatcggcc
     1621 acaaagtcgg cgatggtgaa atcagtttcg tgcccgacgg cggaaatgac cggaaccgcc
     1681 gaggcggcga tggcgcgcgc gacgacttcc tcgttgaacg cccacaggtc ctcgatgctt
     1741 ccgcctccgc ggccggcgat gatcacatca atatatccaa ggcggttcag gtcgccgatg
     1801 gccccggcga tttcggctgc ggatccttcg ccctgcacgc ggaccggcgc aatcacaatg
     1861 tgaagatttg gaaaccggcg gccgagcacg ttgagcatgt cacggatggc cgcccctgtc
     1921 ggcgaggtca caatgccgat gtgctgcggg agcatcggca gggacctttt gcgatccggc
     1981 gcaaagagcc cttccgcctc caggcgggcc ttcagcttct cgaaggcctc ctgaagcgaa
     2041 ccggcgccgg ctgactccat cctgcggacg atgatctggc aggatccggt tttttcatag
     2101 agcgtcactt cgccctgcac gcgaacctgc attccgttgg cgggttcgat gttcagaccc
     2161 gtctggcttc cgcggaaaaa agccgccttg atctgcgcgc cctcgtcctt gagcgaaaag
     2221 tacaaatgcc ctgaagccgc ccgcgtgaaa ttcgagattt cgccctccac ccacacgctt
     2281 ccgatttcct cctcaagcag aaagcgaatg cggcgcgtga tggaggtaac ggaaaaaacc
     2341 tcgggcttcg cgggcgcgtt cacatgtatt ccctcacccg ttcaatcccg gtcgcgcggc
     2401 aggttttgtc atccacggtg accagcgcgc cctcgaaagc caccttctcc gtggcaattg
     2461 aaaatctgga aggcatcccg gtcaggaatt tctgaagaac ggagttcacg tcccggccga
     2521 gaatggagtc cttcggaccg gtcatgccaa ggtccgtgat ataggccgtg ccgccgggca
     2581 ggatttcctc gtcggcggtc tgcacatgcg tgtgcgtgcc ggccaccgcg ctgacccggc
     2641 cgtccagata gcggcccagc gcaattttct ccgaagtggc ctcggcatgc acatccacca
     2701 tgatgattcc tccaggcggg tttttctgcg caaggatctg atccgccgtc cggaacggac
     2761 agtcgtaggg cgacatgaaa acgcgtccca caaggctgat caccgtgacc gggatattct
     2821 tcaccatgac cgtcacgcag cccgtccccg gacatcccgg agcaaaattg gcgggccgca
     2881 ccacgcgctt cgcgtttttc agccacgggg ccagttcttt ctggtcccag gtgtggtcgc
     2941 ccagcgtcag cacatccgct ccagccgcga aaagctcatc gcagatcacc ggggtgatgc
     3001 cccgtccgcc cgcgctgttt tccgcgttgg cgatgatcat gtcgaccccc tgctgctcgc
     3061 gcattctgcc ggcggttcgc gcgaacgcgg tgcgccccgg ctgtccgaca atatctcccg
     3121 tcatcagtat tttcatgaca cttcgacctt aggctatttc gcgtattcca cgcaacgcgt
     3181 ttcgcgaatc accgtgacct tgatctggcc cggatactgc aggtcatgct cgatctgttt
     3241 ggtgatattg cgggccatct gcagggcctg attgtcatca atcttggacg gctcaacaat
     3301 cacgcggatt tcgcggccgg cctggatggc atagctcttt tcaacgccgc ggaagctgct
     3361 ggcgatgttt tcgagatcgg tcagccgctt cagatatatc tgggtggttt ccgcgcgcgc
     3421 gccgggacgc cccgcgctca tcgcgtcagc cgcctccaca agcgtggcat agatgctttc
     3481 cggctcaatg tctccgtgat gcgcgcccac ggcgttcacc accaccggct gctcgccgta
     3541 tttgcgcaga agatccgcgc cgatggtcgc atgaccgccc tcgaccttgt gatcgagagc
     3601 cttgccgatg tcgtggaaca ggcccacccg cttggcgatg ttcacatcca accccagctc
     3661 ggcggccatc atggccatga gatgggccat ctcgatggag tgctgcagca cgttctgcgc
     3721 gaagctgtag cggtacttca ggcggccgag cgtatgtatc agctcgggat ccaccccctg
     3781 caggcccaac tcaaaaacag ccgcctcgcc cgactctctc acaatctcct cgacctcctc
     3841 ggtcaccttg cgggcggtat cttcaatgcg cgagggatga attcttccgt ccgcgatcag
     3901 gcgctcgagc gtaacccgcg cgatctcgcg gcgcagcgga tcgaacccgg agaccacgac
     3961 cacttccggg gtgtcgtcaa tcaggatgtc cacgccggtg gcggcctcca gcgcgcgtat
     4021 gttccggccc tcgcgaccga tgatgcgccc cttcatttcc tcattgggca gcggaaccga
     4081 agaggtgctc atgtcgctga cctggctggc ggcatagcgc tgaatggctc ccgcaatgat
     4141 ttcacgcgcc ttcttttcgg cggtttcttt tgcctcgtcc tgtatatggc gaatcagcac
     4201 gccggcatcg gcccggattt cctcgtccag aatccgggtc agctgggcgc gggcctcctc
     4261 ctgtgacagt gacgcaatct tccccagctc cgcctttttc gtgctgatca gctgttccag
     4321 ctccagctga cgggcctcca tctgtgattt cctgtcgttc agctcctcct gctgctgttc
     4381 cagcttttcc tctttcttct ccagcatgga gagctttcgg tcgaggttcg cggcgcggcg
     4441 catgatgcgc gcctcaagca tccgcagttc atcgcgctgc tccctcgtct cccgctcgaa
     4501 gtcctgctgc gcctgaatct gcatgtcctg ggccttcagt cgggcctctt tcaggatatg
     4561 ctctgcatcc ttgcgggcat cggaaaggat tcttgttgaa tccagctggg cgcggccggc
     4621 acgccgccgt tccagcaggt tgatcagcag ataacccgcc atcagcccca tcagaatgcc
     4681 gacaaacgcc gtccacgtcg gcagccacca gggcacgtca atctggtccc atctcattgt
     4741 ttgtttcccc catccggttg tattttcaaa atccgctctt ccgtgacaat gatgttcacg
     4801 gtctcgtcat gcgcttcgcg cggaacccgc tccagcatct ggcactcaaa ggcaagcccg
     4861 atgcggggcc cgcggaatgc cgccatcagc gcgtcgtagc agccgccgcc gcggccaagc
     4921 cgcccgccgg cggcgtcaaa tgcaatgccc ggcacaatca tcagatcaat ctcccgcgga
     4981 tcagtccagc agggggtgca gggctgttcg gtccggtcct cgccgacgac gagtttttcg
     5041 tccgtccgca gccgcgcaaa gctgtagcgg ccggtctgcg gatcaagggc cggcacacag
     5101 acgttcctgc ccgcctgccg gcagaggctg atcagcggtt cggtctgcac ttccgcgggc
     5161 agggcaagat agcaggccac cgttccggcc gccgcaaact cggaaagtgc cgccgcggag
     5221 ccggccactg cgcgactggc ggaccagatc catgcctgat ccagattccg gcggcgggcc
     5281 gcaatgattt cgcgcaggcg ttctttctgt tctaggactg acgttttcgt gacttgctcc
     5341 tcagtgagtt ccagtgttcg atccgtttgc ggatggtttc ctcatatccg cggttggtcg
     5401 gctcgtagta aatcttcgtg gtcggagcat actcctggtc cacaaagcct tcttcataat
     5461 cgtgcgcgta tttgtagccc gtgtggccga gctgttccgc gcccttgtag gtcgcccccc
     5521 gcagcggcct gggaaccgga agcgtgcggc cctctttcac gtccttcatg gccgcctccc
     5581 cggccatgta ggccgcattg ctcttcggag ccgtggccag ataggtcgtg cactgcgcca
     5641 gcgcgatgcg cgcctcgggc atgccgacaa actccaccgc attcagcact gaaaccgcca
     5701 gcgtgaccgc ccgcggatcc gcgttgccga tgtcttccga ggcgagaatg atcagtcggc
     5761 gcgcgatgaa gcgcggatcc tcgcctgcat agagcatttt cgccagccag tagaccgccg
     5821 catttggatc ggaaccccgc acgcttttga tgaacgcgga gatggtgtcg taatgcccgt
     5881 cttcgtcgtg gtcatacacc acggcttttt tctgaatgga ttcctcggcg gcggcgcgcg
     5941 tcacgcgcac ccggccggcg gcatccgggg gcgtggtcag cactgcgatt tccagcgcgt
     6001 tcagcgcgcg gcgggcatcg ccctcgcaca ccctcgccag gtgttccagc gcatcgtcgt
     6061 ccgcggatgc gttgcgggcc gtcatttcac ggtcacgctc caaagcgcgc cgcagcagtg
     6121 tcaccaccgc tttttcggaa agcgcctcga gctcgaaaat ctgcgaccgc gacgtgagcg
     6181 gcgtgttgat aaagaaaaag ggattgtgcg tggtggcgcc caccagcgtg atggaaccgt
     6241 cttcaacata cggaagaagg acatcctgct gggccttgtt gaagcggtga atttcatcta
     6301 tgaagagaat ggtttcgcgg ccgtaggcct gccgggtctg ccgggccgcc tgaaggaggg
     6361 tccgcagggt ggccacattg gccagcacgc cgctggtccg gacaaaattg cgctgcgtga
     6421 cttttgcaat gacctcggcc agcgaagttt ttccgcatcc cggggggccg tataaaatga
     6481 tgctcccgag gcggtccgcc tcgatggcgc ggcgaagcag cttgccgggc gcaagcaggt
     6541 gttcctgccc ggccacgtcc tccagcgtct gcggacgcat ccgggccgcc aggggctcgc
     6601 cgccggtttt atcttccggt gccctctgtt ccccgccggg attctgatta tctgcaaaca
     6661 gatccatctg tcaccttcgc ggggacgaaa aaaaataccc cgccattgcc gttatgcgct
     6721 ccagctctga tccctcgatc aaagtgggtg ctctgtcgcg aatttccctc ttcccatgca
     6781 tgggcttgag gtctattccc gaacttccga gctcccgttc aatttagtgt tgggtcagag
     6841 tacacatcgc catcacgaac aaggcagggt atttcttccg gcctgaaacc cgtgtttcag
     6901 gcccaagtct cgaaactctt caactgacaa agaaccggcc cggcggaccg tcgcagccgt
     6961 cacccggcgg tccgccgggt tttattggcc ggccattcgc tccaacagcc cactcgcaac
     7021 cgaccaaagg cctccgaact gagaccgccg cactcccgca cggtctccac ttaacgatac
     7081 ccgccgcaag gggaatttca actcccagca gcatcagaac cagcgtttca atccacatta
     7141 atgtggggtt cataccttac cttattcaaa ttctgttcga gcctcagccc tcccggattt
     7201 cgatgccggg aagagccttt tcgagagccc tcttaacgac atcataatat ccgttggcct
     7261 gctcgtccgt caaggtcttt tcggccgatc tgaagatcag tgagtaggcc atgcttttcc
     7321 ttccgggccc ggtgtttttc ccggtaaaca cgtcgaaaag ctcaatgcgc tccagctcgc
     7381 gcggggccgc gccgcgaatg atatccatca ccgcctcgtt ggtcacggct tcgtccacaa
     7441 gcatggcgat gtcgcgctga atcggaggat atggcgccag ctccctgatg cgcttcaggg
     7501 tgaacacccc tccgaggatg tccgccgtgc gaatctccgc gacggccgcc ggatcgctga
     7561 tccgccactc atcgcgcacg gctctgcgga caacgcccag cacaccgatt tctattccgg
     7621 ccacggccac cgccgccgcg tggcccggtt cgaaatacgg ccggtccgtt cgaacaaagg
     7681 aaaccccggt catcctgaga tcggacagca gggcctccac cacgccccgg atccagagga
     7741 aggcctcacc ttccgtcacg ggtttccgct tgtccagact gcaccggccg accggaccca
     7801 tcaggccgat cgccacgcgc tcttcttcgg acggcgcgcc tttcgcatca tggaagaaaa
     7861 catgcccgat ttcaaaaaag gccgcttcag gaagctggcg ggcgcggttg taacccaggt
     7921 tctcaaccat ctgcggaatc agagaggtgc gcaaaaccga ctggtcggtg ctgatcggat
     7981 gaggaagcac aatgcggctc tttgcgccgg ccgggtcaaa cagatccaga agctgcggag
     8041 caaccaggct gtagctcatc acctcgcgaa ggcccagccc gaccagcgtt ccgcggcagg
     8101 caaaccgcgc gcgggtcttg cggtcgtctg cgcccggaac aacccgggtc tggggcgctg
     8161 gagacggaat cttgtccagc ccgtaaatcc gggccacctc ttcgatcagg tccacttcgc
     8221 gctcaagatc gccgcgaaaa gtcggaatgc cgaccgcgca ggaagcttcg tccacggcgg
     8281 tgaccgcaag accaaggcct tcgaatatct gccacacgcg gcccggttca acctcgatgc
     8341 ccagcaggct gctgatcgca tcaaagcggc agacgatact gcgggccgcg ggcggtttgg
     8401 gatagatatc aatgacgccg cgggcgcact gcgcgccggc caggtcaatc atcagtttgg
     8461 ccgcgcggcg gctggcccag tccaccccgc ctatgtccac gccgcgttca aaacggtagg
     8521 atgaatctgt cgagagcccg agcctccgcg aagtttttcg ggtcgtgtcc atgccgaaac
     8581 aggcgctttc cagcaatacg gaagtggtgt tgtcggatat ttcgcttccg gccccgccca
     8641 tcaccccggc cagcgccacg ggccgcttcg catcggcaat cagaagcatg tccggttcaa
     8701 gtttcctttc gatgccgtcc agcgttgaaa gcctgtcatc cggcgacgga agccggacaa
     8761 tgatcctgct gtcggccaga aggcttttat caaaagcgtg cagcggctgg ccggcttcca
     8821 gcatcacgaa attggtgatg tccaccacat tgctgatggg ccggattccc gccagtttca
     8881 ggcgtttctg catccattcg gggctcggag caatgcgaat gttctgcagc actcgcgcgg
     8941 tataacgcgg acacttttcc ggttccttta cttcaaccgc agccagcgca ttcacatctt
     9001 cggaagtttc ttcgaaatcc gccggcggaa gcctgagcgg gcggccgtaa agcgcggaaa
     9061 cctcgcgggc aaggccaatg atcgacaggc agtccggccg gtttggggtg atctccagat
     9121 ccagcacggt ttcgggcggc ccgagtatct ccgacagcgg ggtgccgggt ttgcacgaat
     9181 cgtccagccg catcaggccg ctgtggtcgt ccgaaatgcc gagttcatcc tcggcgcaga
     9241 gcatgccttc ggaaaccact ccgcgaattt tcgacttctt gagggtcatg ccgccgggaa
     9301 gcgtggttcc cgttaccgcg aacggatatt tcgcgccgac ctcaaccaca ggagctccgc
     9361 acaccacaac gtgttcgcgg ctgccgtcaa agaccgtgac cagcttgagg cggtcggcgt
     9421 tgggatgggg attcacggcg cggacttcgc cggccgccac gccggggaaa tcaccgccga
     9481 tggtttcaat gccttcaacc tcgacaccgg aaaaggtcag ccgatcggca agcccctgcg
     9541 gcgtatcgtc gaaatccaca taatccttca gccagcaaag cggtagtttc ataaaattcc
     9601 gtttcgttaa aaaacgagaa gaccttataa acatgccccc gcgcgaacaa gaaaattgtc
     9661 ttgccggatc agtacggaat gggaagtttc gccgcgggat cgtggcgcgg cggcggagcg
     9721 ggtggctctt ccagaagctt cagttcggga atgatttcct tttcgcgcca gggggcggcg
     9781 ctgatcacgc gcggcggttc attcagaacc gcaaagatat cattcgcgca gaaaatccgg
     9841 tcacggcgct ggttcgtggt ttcctgaagg atcccggctt ccagaagctt gtccaccgcg
     9901 cgctgagccg ttgtgaaagc cgcgtcaagc tgtgccgccg ctcctcttac ggtcaggaac
     9961 ggatttccgg ccagcttctc tgtcaacagg agcgccgcag catgccgggg tgcggaaagg
    10021 gtccgcttcc atgtctggac agtctcttca atgcgggcca tgcggccgat caaatcctcg
    10081 cactgccgcg caacgccgtc cagaaagtac agcagccagg gctccgggtc tcccgtcttc
    10141 tggaccgccc ggcagagttt cagatggtcc gcgctggtcg cattcagaaa cgggctgatg
    10201 cccaaacccg gccacggtac catggccccg gataccgtaa gggccgtgaa gacaagctgt
    10261 cccgtgccct gcgccccagg cagttcattc tccagcaggg ttgattcgca aagcatcagc
    10321 ccggcgcgaa tcagaaaagg atcaatcccc ctcaaatgtt ctgctgcgga ttcaaaccgg
    10381 ctggacgccc gacgttcgga cacctggtct tgatgaagca caacgtgcag atccacaaat
    10441 cggcggaccg aaacggaaga aaaccccggg gattcccccg cggcctgatg aaaagcgttc
    10501 atggcggatt cgacggacga cccgtatcgt gcgctctccg catggctctt caagaccgca
    10561 acccaatctt tgcggcagag ggctgaaacc tctcggatca gcagcagatg cctgatttcc
    10621 ggctccggca tacgatccat cgtgccgccc agccgcccca tgagataatc cgcctcagat
    10681 aagccttgta gaacgttcct gttccagtca attttcttca tgggtttata ttattattga
    10741 attctatgat tttcaataac aatatattta atattattag atttcttatt aatcaataat
    10801 atctcatatc ccgataatat gaatttttac gatgtctgct gttttagaga tttttcgctg
    10861 ctttaaatcg gaacatgcgg gccgaagaat catttttctt catttatcga cccctcggga
    10921 taaccgcgcc gcgatccttc gtacgcactc agcccgtcag gctggcggat tctagggcgc
    10981 aggcgcgcgg ggcttcattt ccgcatgtat cgcggttaca gaagaggact tgatctctac
    11041 acggtcattg aaaggcgtcc agccgtcctt gaacacagca atggtgtagg ttcccgccgg
    11101 cagcttgacg ctgacgggga cacgcccggc ggactttgaa ttcacatagg ccgtggcgcc
    11161 atctgtgctg gcggtgatgt taatggttcc cacagtggag ggatccgccg cactgccgga
    11221 tgccatgatg accgaagcgg gagcctgact cgcgctccgg gtctcatcca aaggctcgcg
    11281 ggcaacctgg gtcagattga gcttcagcgt caggtcactc ggccagttct ccgcctcaga
    11341 ccagtcaaga ccgggcgaag gtagagagag tatcctttcg ttcacgatct tcggccggca
    11401 tccgggaagc acggcgctgc agctcgcaaa aatatcccaa gttttgtccg tatccagaag
    11461 cgcatagcag acatctcccg gcgcccatat gctgaggcgc gtccagcgcc tgaagcccgc
    11521 cgtgcgatcc cacttcaaac ccaccatcgt ccggcacggc gtgaccccaa tcatgtcgcc
    11581 gactttcttt cccccgtcgg tgagcgcata aaccgcggcg cccgagggct cgctttcgat
    11641 ggtgagatag aggatgtgat cgaaaactgt tctggcctgc gattcctgtg ccgcgccatc
    11701 cgacgcacat agcatccatg ccgccagaac caggatcgcc gccgctgtcc agcttttcag
    11761 tttcatggtc cgtacattct cctgtttgga aaacatacca gacaaggagc ccgcccgcca
    11821 gaatttttca agggaattgc ctaaaaagct tctcatttta gtagacacga acacttcaaa
    11881 tgtatatgtt gcgtcttgtt ttagtctttg aagaaaagac ggacagagtt ttaaaaactg
    11941 caaaaaaagg gagatacgca gatggcaaaa gcaaaaacta aatcgcagat tatcgccggc
    12001 atcgccgaag acgctggact gaccaagaag caggcggccg ccgctctgga ttctctggta
    12061 agccaggcct acaagggcgc caaggacgga ttcacgattc cgggtctggg caagctggtc
    12121 aaagtgaaac gcaaagcccg catgggtcgc aaccccgcca ccggcgaagc gatcaagatc
    12181 gcggcgaaga cagtcctgaa attccgcatc gcgaaagcgg cgaaagacgc tgtcctcggc
    12241 tgatcaaaat cctgttgatg aaaaaggccg gttcaccgaa ccggcctttt tcttttatgc
    12301 gccgctattg acggggggcg cggtacgtgg tatccaatgc ggttttcagg ccagtattga
    12361 gcagttttcg ataaggagta aacagtatgg caagagtata caatttcagt gcgggaccgg
    12421 cgacgcttcc cctggaggtg cttcaggagg cgcaggccga atttgtggac ttcaagggca
    12481 cgggcatgtc gctgatcgag gccagccacc gcggcaaggc ctatgacgcc gtgcacgagg
    12541 aggccgttgc gaatataagc aagctggccg gactgacgga cgattacgcg gttgcattca
    12601 tgaccggcgg cgcctcgcag cagtttgcgc agatcccgat gaacctgctc ggcgaaaacg
    12661 acaccgccga ctacgtgaac accggagcct ggaccaagaa ggccattgcc gaagccaagc
    12721 tccagcgcag catcaaggtc atcgccgatg tcagcaagga atctccgctc cgcatgccgc
    12781 gcgaggacga gttcaaggtg aacaaggacg ccgtctatct gcatctctgc tccaacgaga
    12841 ccatcgccgg gacgcagcta aagaagttcc cgaaagtgga cgtgccgatc gtggccgata
    12901 tgtcctccga tatgttctcg cgcccgttcg acatccggca gttcggcgcg atctacgcgg
    12961 gcgctcagaa gaacatgggc cccgcgggcg tgacgatcgt gatcatgcgc aaggacctgg
    13021 ccgcgcgcgt gtcggaaaag gttccgaaga ttttccgcta cacgacgcac atcgagaaca
    13081 actcgctgta caacacgccg ccgacctttt cgatctatat cgtcatgctg gtcacgcgct
    13141 ggatcatgaa gaacggcggg ctcgcgggca tcgagaaaat gaacgccgcc aaggccgcga
    13201 aaatctatga tgccattgac gccagctcgt tctaccgcag cccggtggcg aatgattcgc
    13261 gctcgttcat gaatattacg ttccggcttc cgagcgagga gctggaggcg cagttcgtgg
    13321 ccgaggccaa gaagctgaaa atggacggcc tgaagggcca ccgcgacgtc ggcggcatcc
    13381 gcgcctcgat ctacaacgcc ttcccgatgg agggcattga cgcgctggtg cagttcatga
    13441 cggactttga gaagaaaaac ggctgagaaa acacgccgcg cctgtagttc aatggataga
    13501 gcgtcagcct ccggagctga agatctgggt tcgactcccg gcaggcgtac cagcctcaga
    13561 gccgccccgc acgcgcgggg cggcttttgc tttcggaaca gttaaaggaa atcaggagaa
    13621 atcatcatgc cgacaaagaa aaaaaatgat cacaggcggg cggacggtcg gaaactggac
    13681 gaactgagga aaatttgcat cacgcccggc gttgcgccgg cggccacggc gtcggtcctg
    13741 atggaggccg gacgaaacaa aatcatctgc gccgtgagca tcgaggagga tgttccgcgc
    13801 tggatgcgcg cgcagaaaat cacagggggc tgggtgaccg cagagtacag catgctcccc
    13861 tacgcctccg aagaacgcaa gaggcgcgat atcaccgccg gaaagattga cggccgctcg
    13921 caggaaattc agcggctcat cggccgatcc atgcgggcag tcattgatct ggaacagctc
    13981 ggcccgcgca ccgtatggat agactgcgac gtcgttcgcg ccgacggagg cacacgcacg
    14041 gcatccataa ccggcgcatt cgtcgcatgc cggctggccg tcaaccggct gatcgccgag
    14101 ggcaggctgg ccgtgaaccc gatccgggag gcactggcgg ctgtaagcgt cggcattgtg
    14161 ggcggccagc ccatgctgga cctcgcctac accgaggatg tcgcggctga gacggacatg
    14221 aatgtcgtga tgacggaatc cggaaagttc gtggagctgc agggcacggc ggaggagcat
    14281 ccattctcaa aacatgaact gtccgctctt ctgaaacttg ccgaaaaggg tgtcgcggaa
    14341 ctgctgaaaa agcagcggga cgccatccgg agcgcaaaat agcggccgtc agttgatcgg
    14401 gctcgggaac ggcaggccgt tggccagcac ctgcgccagg aatacataga ccagcacgca
    14461 gagcacaatt gtcgccgcga tcgaaacgat cgagaacgca attccggcct cttccttctc
    14521 cggcttcgcc gggatttcaa cctgcgcagc ctggaagctg gaagtcaccg gccgctgcag
    14581 agaaagcttt ggaactccgg catccgatcc gtcgggccgc ttgattttta cggttttctt
    14641 tcgcgtcggc ggacgcaccg cgtccggcgg aagttctatg cgtgatgtct cgctcttctt
    14701 gccttcggcc atcatggcgg aacccggctg ctgaccgaga accgaatggg gaaccatggg
    14761 ttccggcgga cgcgccggcg gcttgcgggc tgtggaagtc gtaaccggac gcttgatccg
    14821 aatggtcgcc ggcgcaccgg cgctgatttt ggttgtgtcg cgcggcgagg gaattgccga
    14881 ggatatgtca atgcgggcgg tctgcttttt gggctgggtc tgaggggctt cacccaggtc
    14941 gtcttcgatg ttcatgaaca tcgttgcatt cttggccaga ctggcagacg gcgcaggcgt
    15001 aaactcatcc agcgtttcct ggggagccgg cggcacggcc gaagcggtgc tgatgcggga
    15061 agttttcttc ttggcatcca ccgggggtgg cggctgcggt ttttccgcct ccgcggaatc
    15121 cacatccatg aacatggtgg ctttcttggc ggcaggctcc gccgatgcga tggcatccac
    15181 gtccataaac atggtggcct ttttcccggg ggccgggggc acttcttttt ccctgatgtc
    15241 cgaaacagga gctggcggct tctgcagatc tttgcgggcc gattcgatct gagccgaggt
    15301 tttctggatc gcctgcttga gcgtgccaag atccgcttct cccgccggct tggccggcgc
    15361 aggctgcgca gcttcctccg ccgttttgtc ggccaaatca atgcgcggtg ttttcgccac
    15421 gggacgggcg ccgattttga tcgtgggccc ggccggagag gaccctgccg ccgcgggctt
    15481 tttcagtgcg ggtttcttaa gggccggctt cggagcgggc gcacccgctg gcttgctgtt
    15541 cggctgtgct gacggcggtg ttttgttttc ttcgctcatg atcatctcct gcaacaatct
    15601 acgcgcatat tagagtggtg tgcggaacgg gtcaaaacaa atgtatgctc ttcttgctga
    15661 ataccgggga tgatctgcta tattgttact gtacatggaa aaatcaatat gctgaagatg
    15721 ccgtacatag ccggaatcct gatcgcgctg gtcgcggcgg ccagtctcct gaccggatgc
    15781 aagccatccg gcggaggcga ggaggaaatg atgccgcagc ccatcggttt tgatctgccg
    15841 ggcgccggca gtgcgccggt gcacgatgtg cgcgccatgt ccgacctcgc gctgcaaatt
    15901 gttagggaag ccccggccga agagggcggc ggcgccacaa ccggcgggca gattgaggag
    15961 cagaccgccg cactggaggg aagcgtgatg tttccccgat ggaccgcccg gcgcgtgggc
    16021 gccaacgact acgaagtccg gttcaccttc acgcacatag acaaccagta caacatgaac
    16081 aagacaggat tctcgtggat cgtcaacgtg gcgcagaaga ccgccggtcc ccggcgggtg
    16141 cttcggccgg aggattttga cgaggccggc ggcgccatgc gaacgccgtg cgactgggag
    16201 ccgctgatgt tccgcgaaag cagtgcgcat gaaaaacagt agccgcgaac aggtcctgaa
    16261 aacggcattt gaacagcatc ttcctgagtg gtttgccctg gtccgccggg ccatgccgtg
    16321 gcgggtcaac cggacgccct accgggtgtg ggtggcggag atcatgcttc agcagacgcg
    16381 cgcggaccag gccgcgccgt attaccggcg cttcgtcaga acttttccat cggtgcgcgc
    16441 gctggcccgg gcatctcttc gcgatgtgct gaaggcgtgg gagggcatgg gttactatgc
    16501 gagagcgcgc aacatgcaca aaaccgcgca gcacctcatg aacacccgcg gcgggaggtt
    16561 tcccagaacg tatgaggagt tgaagcagct gcccggaatc ggcagctata cggccgccgc
    16621 cattggaagc cttgccatga atctggatgt cgcggtggtg gatggaaatg tcatccgggt
    16681 gctgtcccgc gtgtttggcg tggatgagcc ggtggacact gccgcaggcc ggcgcatcat
    16741 tcagggattt gccgaatcgc tgctgccggc aggccgcgcg gcggaattca acgaggccat
    16801 gatggagctc ggcgccctgt gctgcactcc gcgcgatccg cgctgcgaaa agtgtcccct
    16861 gtccgccgtg tgccgcgcct gcgggctcgg gcgcagcacg gaatatcccc gaaagcgcag
    16921 aaggaaagcg gttccgcaca aggtggtcgg cgcgggagtg atcatggacc gccgcggacg
    16981 ggtgctgatc gcccagcgcc gcacacagga catgcttggc gggctctggg agtttcccgg
    17041 cggcaagaaa gagccggagg aaaccatgcc cgaatgcatc acgcgcgaac tccgggagga
    17101 gctcggcatt gaggtgaagg tcggcgagct ggtgacggtg gtgcatcacg cctacagcca
    17161 tttcactatg gaactgcatg cgcaccgggg ccgggttgtg aaaggccggc cgcgcgccat
    17221 agagtgcgct gattgcgcat gggttctccc ggacgaactg cgcgagtatc caatgggccg
    17281 tgcggatcag cacatcgtcg cggcgcttct tcaagaagac gccggctgat cattcagctt
    17341 ctgcgctgaa tcagaaaccg ggcaatatct tccagtgtgg cggtctgaag acccgtttcg
    17401 cgaactgcgg caacggcttc atcaataagc tccgcagcct gtctttggga ttcccggaca
    17461 ccataaattc cgcaccatgt actcttgccg cggtcccggt cacttttcac gggcttgccc
    17521 aattcctcgg gcgtggaggt ttcgttcagg acatcgtcca tgatctggaa cgcgaggccg
    17581 gatttttcgc cgtacacgga aaagctgtcc acaacttcag gcggcgcgcc ggccgcgatg
    17641 gcacccatcc gcaccgcagc gcggatcagt gcggcagttt tatgcagatg gatggaccgg
    17701 acttcctcgg cgccgagact ccggttttcg ccctgcatgt cctccatctg tccgcgaatc
    17761 atgccgcggc ttccggcggc ctgcgccagc tcggtcacca gctgcagggg catccatggc
    17821 ggaggggccg cacagcggcc catcagttca aacgccaggg taagcagggc gtcaccggcc
    17881 agaatggcgg tcgcctcgcc aaattcaacg tgacaggtcg gacggccgcg ccgcagatcg
    17941 tcatcgtcca tggcgggaag atcgtcatga atcagcgaat aggtgtgcag gatttcaagg
    18001 gagagcgccg gcagcagggc gtccgccccc ggatttactg cttcggcggc ggccagacaa
    18061 aggatgggtc tcaggcgctt cccgcccgcc agcatgctca cgcgcatggc ctgatgaagc
    18121 cgcgcaggag gatcgtcttc acgcggcaga ttccgatcaa gagccgtttc gacaagggcc
    18181 tttccccgct gcagatactc tttaatgtcc gtcattcctg aaaccctatc cgaccggcgg
    18241 cgcgatgcaa gacgaaagcg atgccggaag cgttcaggtc cgcacaagtc tattgtgcgg
    18301 tttccggccg acgagaagcg actgaaggat agcgtacagt tcctccgggc cacttgctgc
    18361 atggagccgc ctgcgcatat ccgccgaagc catgcttgag gcaatggcgg aaagcgtctg
    18421 cacgtggata tcctctgttc ccatgggcgt ggcaaagaaa aacacgtctt cgaccggaac
    18481 cccgtcgggg gcatcccagt caattccctg acgggagcgg ccataggcaa tgacgggatt
    18541 tttaaggttg tcaattcgcg catggggaat ggccacaccc tccccgatgg cggttccgaa
    18601 ctcatcctcg cgccgcatgg ccatttgcac aagaatatcc cgggtgccgg aaccggcctg
    18661 gcgctctatt cgttcggcca gttcctgaac ggcctccgcc cgggaagccc cggccagatc
    18721 ggccacaata ttgtcgggct ggaggaaacg ggcaggcgtc actgcagcac gacggtccag
    18781 agagcgggcc agccacggac ccatcagcat ggaagaaaac accgcgctaa ccactatggc
    18841 aacgaatatg gccggagtga tcagatcggc ctccagcgcc agcagggcaa caacaatctc
    18901 catcattccg cccggcgtgt gcgcaatgga aatcagatcg cggttgatgc gggaaactcg
    18961 ggcccaggtc acgccaaacc atgcgcccgc atagcgtccg ccgatgcccg ccgcgcaaat
    19021 cagtgccact acaggaaggt tgaaatcggc aatataatcc attttgagcc caatgttcgc
    19081 aaaaaacagc gggacaaaaa gcgaatgaac catttgagaa atcacaccgc gcgtctgttc
    19141 ggaaagactc ttcgcttcgc cgacgaccac accggcaata aaaaagccga agagcgcatg
    19201 aatgcccagc catgaagtga ccactccaaa caggaggccc agcaggaccg tgatggtcag
    19261 cgaagtggcc ggttcgggaa atttgccgcg cttcacggca tcgaatacac gcgttgaaac
    19321 ggggcggcca agcgtcagcg caagggcggc aaagccgaca gtgctggtaa agaccatgaa
    19381 aatgggcccc acggcggctg tcgaagtcgt gaatgttccg aggatgatcg tgaacagaac
    19441 ccaccccaca atatcgttca ccgccagcgc cgacattacc aggaatccga gatcggcctt
    19501 caacagcttg aggtcatgaa gcacgcgggc ggccaccggc atggcgctga tcgtcatcac
    19561 cgtggccatg aaaagcgcga aaataatccg ctgttcggga ttcaccagaa agcggtccgg
    19621 aaggaaataa accgcggcga accccaccgc caccggaata atgatgtcgg atatcgcaat
    19681 caaaaatgcg cttccccgct gccgccatgc gatggagaag tcgatttcaa gccccgtgtc
    19741 cagaaggaga aacaggacgc caacccatgc aacggtgtcc agcatggtct gctgaatggc
    19801 gtccggaggg aacagcgcca gctgcagctc cggccacagc cggccaagaa tcgtaggacc
    19861 cagaaaaatg ccgacgagaa tctcgccggt cagcgcaggc tggccccagc gccgaagaag
    19921 ttccccagcc ccgcgcgcac accccataag aatgaaaatc tgaatcagaa aaagcagaat
    19981 gtgcgattca tcgagataat gcattggctt ctctcaaatt gctgcagcac cggataccgc
    20041 cagcttcttt tttatcagta tcaccgattt gctgtccgcc gtcaattact tcccggacgg
    20101 ccggtcatcc tgacggcaaa aagactttat tgaatgaact ggccgtgata aaaagaaccc
    20161 atgaagggaa agctcattat tctttcgacg ctgatccttc ttgccgtcct tccacttgcc
    20221 gggggtctgc tgaaaggcct tccggaggat ttcttttcgt ttccgccgct tgtcgaacat
    20281 tcgccggttc atgcgccctt ctctcccccg gtcttctcca tgtttgcggc actctgcgtt
    20341 ttgaccgccc tgtttttcct ttttccattt tatttcggat tcgcccggca gactgcttct
    20401 ccgccttctg ccggggggcg tttccccgtc tggggccgcc ttgggctggc gctgatgctc
    20461 ttttcctgga tctgcgcatg gggacagttc gactttctgg gccgcgcgcg actgcacacg
    20521 tttttcccgc tgtgggtcgg cttcattttc acaatggacg ggctggtgtt ccgcagaacc
    20581 ggccggtcgc ttttttccgg gcgcagagcg gcctttttca ccctctttcc ggtctcggct
    20641 gtctcatggt ggtattttga acttctcaac cgcattgtgc agaactggtg gtatccggat
    20701 cgaaccgatt ttggtcccgc gcattatctg atctattcct cgctctgctt ttcaaccgtg
    20761 atgccggcca tttttgaaat ccgtgaatgg ctgatgagct ttccgcccat gcgcgtccgc
    20821 tatgcccgcg ggcccagcat ccggcttgcc ccgcttccgc ttgctttggc cagtgcggtc
    20881 ctgctggccc tgataccgct tttccccgat ccgctgtttt tcgtggtgtg gattgcgccg
    20941 ctcggtttgc tcggctcagc gctggctctc gcccgcgtga aatcaccctt cagcgcaatg
    21001 accgaaggcg actggaccgt ggcggtcaat ctggggctgg cggcgctgat ctgcggtttc
    21061 ttctgggagc tgtggaactt ctacagcacg ccctgctggg aatacagcgt gccctatgtc
    21121 acccgggcgc acctcttcgc gatgcctgtc gtgggctatg ccggctatct gccctttggt
    21181 ccggtctgcg cctgcttctg gctggcctgg accgcacttc ttcccggcaa aatccggatc
    21241 aggcgggcgg gcccgccctc tctcgcgccc ggcgctccat gattccgcag taaagcaccg
    21301 gaacaaccag catggtaatg acctcgatga gcatgccgcc gaaagacggt atggccatgg
    21361 gcaccatgat ctcggagccg cggcccgtgg acgtgagaac aggcagcagc gcgagcagtg
    21421 tggtggcggt ggtcatcagg caggcgcgca cgcggcgtgt tcccgcttcg atgaccagcg
    21481 cccggatttc atctgtggtg gtgtgcggct tgtcccggaa tatctgggtg agataggttc
    21541 ccatcacgac cccgtcatcg gtggcgatgc cgaacagggc gagaaatccg acccagaccg
    21601 caacgctcaa attgatgaca tgcacctgaa agaggctgcg catctccacc ccgaaaagac
    21661 tgaaattgag aaaccacggc tgcgcgtaga gccagatcag aatgaaaccg cccgaccagg
    21721 ccacaaacac tccggagaaa atgagcagtg aagtggacac ggatttgaac tgcagataga
    21781 tcagcataaa gatgaagaac agggcgacgg gcagcaccag agcaagcgtt cgggaggcgc
    21841 gaagctggtt ttcgtagctg cccgcaaagc ggtaactgac cccgggcggg agactgaact
    21901 cgccgctggc gattttagat tgcagatggg cctgacactg ttccacgaca tccacctcgg
    21961 ccgtgccggg ttttttatcg aacacgacgt agccgacccg aaatgtatct tcgcttttga
    22021 tgttctgcgg accgcgcaca tatttgattt cggcgagttc ggtgatgggg atctgctgtc
    22081 cgccggttcc cggtataaga atcttgtcga gcgcctcaat gctgtcgcgc agctcgcggc
    22141 tgtagcggac gcggacggga aaacgctcgc ggccctcaac cgtgcccgtg acttttctgc
    22201 cgccgatggc gatttcaacc acatcctgaa acttgcggat cgggatgcca taacgggcga
    22261 gtgattcgcg gtcgggcacg atctccagat agggcttgcc gacaatgcgg tcggcgatga
    22321 ccgcggacgg ttccaccgac gggacctctt ttagaaactt ctcaatctgc aggccgacag
    22381 cttcgatggt gccgaggtcg ggcccctgca cctttacgcc catgggggcc cgcatgccgc
    22441 tctgaagcat aacgatgcgc gcggcgatgg gctgcagctt cggcgcggag gtggtgcccg
    22501 tgatttttgc ggcccgcacg atttcgttcc agatgtcgtc cgggctgcgg atttcatcgc
    22561 gccactggcg atagggccgg ccgcgcgaat cttcaatcag ctcgccggcc gcatcccgaa
    22621 cgaactcacc attcttgtat ttgtagctga tccggcgtcc gtccttgtcc tcgcggtatt
    22681 cagacttgta gttgatgacg gtttcgatca ttgagatcgg tgccggatcg aggggcgttt
    22741 cggcgcggcc cagtttgccg acgacggact cgatttcggg aatggaacga atggctatgt
    22801 cctgcttttc aaggatgtcc agcgcctcgc cgatggacgc atgcgtcatg gtggtcggca
    22861 tccagaggaa ggatccctcg tcgagactgg gcatgaattc ctttccgaat ccgggaaaga
    22921 tatgggcgat tttctgggcg ggcacggttt ttcttaccgc atccggaagc cagccgaaga
    22981 gtttttcgaa tccgagccag acggtcgcgc cgaagactaa gagaaccagc ggaatcgcga
    23041 gaaaagtctt cttgtgtcgg agactccacg cgagcatctg cggatagaaa tgctgaaaaa
    23101 cccggaaaaa tccgagcaag ccgccgataa gcagaaccac aaagatcaga ttgcggatca
    23161 ctccccgctc cggtccgagc ggctcccatt caaacgtcag ccaggttccg acaagcagaa
    23221 caatgagcgt gatcggcgcg tatttcttca ggagagctgg aaagaatcgc cgcggcggat
    23281 gactcggcgt gcccggtgtc cgcgtccggc cgaagaaggt gtgcgcaagg acgggcagta
    23341 tcgtgagtgc aataatgatt gaggcgatca gcgcaaaggt tttggtgaag gcgagcggct
    23401 tgaaaagttt gccttccgcg ccgatcatgg tgaagaccgg caggaaactg acgacggtgg
    23461 tcagcaccgc agtcagcacc gcgccgccga cttccgcggc ggcccggtga acgacttcga
    23521 gcccggactc ggacgggccg gcctcatcca gatgcctgag aatattttcg cagatgacaa
    23581 tccccatatc cacaatagtg ccgatggcga tcacaatgcc ggagagtgcg acaatattgg
    23641 cctgcacatt gaaaagcttc atggcgatga agcagagcag aacggccagc ggcagcatgg
    23701 agctgatgac cagtccgctg cgcagatgca tcaccatgat cagcacaaca atgatggtga
    23761 tcagaatttc ctgcccgatc gctttgttga gggttccgag tgtttcgtag atcaggccgg
    23821 tgcggtcgta gaagggaacc accgtgacct ggctgacggt gccgtccgcc agagtttttt
    23881 tcggaagcgc atcccgggtt tcggcgatcc tggccttgac gctggaaatc accttcagcg
    23941 ggttgtcgcc gtagcgggcg accacgaccc cgccgacggc aggtgcgccg cccttgtcga
    24001 gcgccccgcg ccgggcggcc ggtccgagct gaacctgtgc gacatgtttg acaagaaccg
    24061 gcacattgtc ggccccgacc cgaatgacac tgttttcaat gtcggcgagc gttttgatga
    24121 atccaacgcc gcgaatgaaa tattccaccc tgttgatttc gatgctcttg gcgccgacat
    24181 caatgttggc gccctgcacg gcgcggtaga cctcgtcaat gctcacgccg taggcgcgca
    24241 tggcgtcggg atcaacatcc acctgatatt cgcgaacata gccgccgata gaggcaacct
    24301 cggccacccc gtcggcggag agcagccagt agcgggcgta ccagtcctga acggtgcgaa
    24361 gctcctcaag atcccatccg ccggttggat tgccgtcggg atcgagacct tcgagcgtgt
    24421 accagtagac ctgacccagt gcggtggcgt ccggaccgag cttcggctgc acgccgtcgg
    24481 gcaaggtgcc ggacgaaagg ctgttgagtt tttccagcac gcgcgcgcgc gaccagtaga
    24541 actccacatt ctccttaaaa attatgtaga tcgaagaaaa tccgaacatg gaatagcttc
    24601 ggattgtctt cacgccggga atgcccagga gctcgacagt gagcggatag ccgatctggt
    24661 cttccacatc ctgcggtgag cggcctgtcc attcggtgaa aacaatctgc tggttttctc
    24721 caatgtccgg aatggcgtcc acggccacgg gatagcgctg gaggccgccc gccttccagt
    24781 cgaacggggc caccagaatc cccgcaaaga tgacggcaaa cgtaaacagc gagacaacca
    24841 gccggttttc gaggcagaag cggatcagcc gcccgacggc cgaccgctgc tctctggttt
    24901 gttcctgatt aatgggcatg cagatcttcc agtctttcgg gggctatatt tttctcgcgc
    24961 atttcctgaa gatatttttc gggatttttc cggaaggttt catcacagcc gccgcagcag
    25021 aaatagatgc gcattccgtt gtagtcggca aagaagtctt tgttgatttt accccccatg
    25081 accggacagc gcgtttgggg caccggcccc atatctttca tttcgtgagt tttccccggc
    25141 tgcgacatca tgcttggttt ggcgcggatc tgcatttcgg catcgagctt gaacgcgccg
    25201 cgggtcacca cgcgctctcc ctctctcaat ccccccgcaa cgatatagaa gtctccgacc
    25261 tttgccccaa gctctgtttc gcgcccctcg taggttggct tttccctgcc gggcacttca
    25321 acataaacca cggcgcgccg gccggttttc agggcggccg ttgcgggaat gacaagcggc
    25381 gcgttcgttt cgttttcggc ggcgtagccg agcgactcag cggtgaccag cggcatgccg
    25441 cacacatcgc acacgccggg gccgtctttc acgatttccg gatgcatcgg gccgatccat
    25501 tttccggcaa ggtcgggatt catcacccgc ccgtctgccg cgacgatcgg gcgcgccacg
    25561 gcgcggacaa acatgccggg tttcagtttt ccgtcttcgt tgcccacgat cacccgcacc
    25621 ttggccgtgc gggtggacaa attgatgaca ggatcaataa atgaaatcgt gccgttgaag
    25681 gtttcaccgg gccaggtctc gacgctgaac tccacttctc cgccgtaacg gagccgggaa
    25741 agatcggatt cgtaggcatc aagctgaacc cagacgcgcg acagatcagc gacggtatag
    25801 atgcggtctc cggttttaac atagctgccc tcctgtgcgt ttttatgaat gacgatcccc
    25861 ccgaccggcg aatagatcgt caggtgatcg gtgggcaccc cgcgcgtttc gatctcctga
    25921 atctgttcgg gggtgagccc ccagaggcgc agtttttcgc ggactgcatc aacggtggcc
    25981 tgcgagacat tgcggacaat ggaactctcg ctttgatcca gagacttgac cgacttgatg
    26041 gcctgaagca gttcctgctg cgcgctcagc agttccgggc tgtagatgga ggccagatga
    26101 tcgcccgggt tcaccttcac gccggtataa tccacaaaca ggcggtcgag ccggccgggc
    26161 atgcgggcgg tgatataggc ggtgcgcgtt tcgtcataat ccaccttgcc gaccatgcgc
    26221 acctcggcgg ttacaaattg gcggcgcacc tcggtggtct caagttccat ccgcgcggcg
    26281 gcttcagagc tgacactgat ttcgcgtccg ccgccctcgt cgcttccgga atcaagcggg
    26341 atcaggtcca tgaagcagat cggacacttc ccgggcttcg gaagtttgat ctgcggatgc
    26401 atggagcagg tccaccaccg ggcttccgtt tcggcgacgg ccatttcaga cggcgccggg
    26461 gccccgctcc cggttccgcc ggtgcagcgc ccaagcgcaa aggcgatcag cagggcgaac
    26521 aggccgcaaa tcagtgtctt cttcggaatt tctccttttt tcatgtgcgg cccctttttt
    26581 tacaaatcgc gattgatcag catttcgatt tccgccaggc ggatctcgcg gtcggtgaaa
    26641 gcccggtcgg cggcgagctg aaattccagc agcatccgtt cggcgtcaat cagcgcggtg
    26701 aagctgtcgc ggccggtctc aaagctttca cgggtcactt ccagcgcctg catggccttg
    26761 ggaaccagcg tgtcgctgta cagattcatt tttctctcag cgtcccggaa attaaaaagc
    26821 gccatctgca gatctgcgga cagggtgttc tcggcggccg ttcgcagcgc ttccgccgcg
    26881 gcacgccgga aatgcgcttc ctgttcggcc gctttcaatt tgccgaacca gagtggaagg
    26941 ccgacggaga tggtggccat gatcggatcc ttgccgctgt cggatacgcc cggcatgaga
    27001 gcctcgtcgg tctgggtgta gtccagtcca agcataatat cgggatagcg ggctttgcgc
    27061 gccagttccg cggatttctg ctccttccgg atcacggcct ccagccgcat aatttcggga
    27121 ctcgcttcgc tgaaccactg ccgaacttca tcatcggtaa acgcggcggg ggttacgact
    27181 ggagagtccg gccacggcaa aacggggtcc tccctccggt tcagcgccgc gctcagtctg
    27241 gccgacagcg gctcccgcag ggcctccagt gtccggagtt tgtcatccag tttccccagc
    27301 tccacctgag cctgaatcac cgggttttgc tggatttctc cggtctgcaa acgagtgcgg
    27361 gccacctgtt caatctgctg cagaagcgcg agatgctgcc gggtgatttc gatggagcgg
    27421 ttcaaataat gatattcaaa gaaggcagac ttgacgcggt agaacagctg ccgtttggcc
    27481 tgttcgtagc gctggcgttc cgcctccgcc gcctccgcgg cggcttcgcc gcgcagcttc
    27541 agcgtcccga accaggggaa aacctgtgaa aggccgaggc gctgcttctg cgggccaacg
    27601 cgggtttcga cctcttcgac aaaataagca tagttgaaac gtggatcagg caaagccctg
    27661 acctgcggga ttctctcgag ctccgctttc cagcgattga aggccgcctc cagctccgga
    27721 ttgttccgcg cggcggcggc tagatagtcg cgcaggtcat ccgcttttgc gccggcggca
    27781 catgcggtca gcatcgcggc ggcggccagt aaaagacggt tgaacttcat ggaccatctc
    27841 cgtatctcat cttcgtgccg gcgagatata ttcccgcttt tgtcataata aagtataata
    27901 gtagacttcc cattaggcaa cgtcagaaaa acggttttgg gggcgttttt gcttgttttt
    27961 ggccgttttt gagcgttttt taacgttttt tggccnnnnn nnnnnnnnnn nnnnnnnnnn
    28021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnttttt aacgtttttt ggccattttt tcgcttcaga cggacgtcgg
    28141 cgcgggcgtt tctgccgcag tcgttgcccg aaccccgatg tacagataaa ccagcgtcag
    28201 gacaaaccac gcaatgcccg gaatgaaaaa gccaatcggt gtaagcatga aaaccgtgtg
    28261 cagaacgatc gcgggcgttc cggccatgac ggacaggcgc aggaccgagg catacggcac
    28321 ggcggatttg gcaaaaagca gccctatcag cgcatagatc agcatcttga tgaccttgcc
    28381 gatgaaactg gagatcagca cggccgcata aacagacggc atgatccatt ttctgcccag
    28441 cgccacccag ttcgtcattt tctcccgggt gagcgcaaag tttttgattt ctgcaaagct
    28501 gtaggatcgc gtttccaccg cgctctgctt catgatgacc ttcgtttcgg tcacaagcac
    28561 aaaggcatcc tgcccttcca gcgaggtgta tttcccggta gtgtcgaaaa tggccagtgt
    28621 gttggtggaa tgcggcatgg ggatgaaaca gggctggtcg gcgtggataa ccgcccgccc
    28681 gtccgtaatg gtcatttcag gaatgcgttc gatcaggccc ggcgcatagg agtccaccgc
    28741 ctccgcaaac ggcccgtgcc atgtcatcac caccggcacc cagcagacca gcagaagcag
    28801 aaagagataa ccgaaggcgg tcccgcgatg gttgcggccg atatcggcgt agaaatcttt
    28861 cgagaaaaac gcgtaaagcg gcgcctgaaa cacggagtat tttttcattt cgtcctcctg
    28921 atctggaaaa tctctgttta acatggtcgc cgatcaaaat caatggctct tcggggaaga
    28981 cagattgtct tttcgggggc gatctgatag cgtgcggact catgaagaaa aattcataca
    29041 ccttcaagct cacgccggag cagcagcgcg agctggagca ggcgcttcgc acgggcaact
    29101 accggccgaa acaagtgccg cacacccgga ttgccgtgga ggggccggac tacaacgtgg
    29161 cgctctacac cagcgggaaa tgccttgtgc agggcaaggg cgcggaggaa tgggtgacct
    29221 tcacgctgga gccgtatatc ctccgcgagg tggtcaccgg atacgacaag attctgcatc
    29281 ccgaggccat tgaagcccac atgggcattg acgaaagcgg caagggcgac ttcttcgggc
    29341 cgctggtgat cgccgccgtc tacaccgacg aggctattgc cgacgcgttt cgtgaaatgg
    29401 acgtgcgcga cagcaaaacc atcaccagcg acgccaaggc cgaaaaaatg gccgaggcca
    29461 tccggaagat tctgaaaggc aaattcgcca tcgtaaaaat cggacccgaa gcctacaacc
    29521 gcctttattc aagcatcaag aacgtcaacc gcctgctggc gtggggccat gcgcgggcca
    29581 tcgaaaacct gctggacaag gtgcccggat gccccaaggc catcgccgac cagttcgggc
    29641 cgaagcagac cattgaaaag gccctgctga agaagggccg gaagatcaca ctcgtgcagc
    29701 gccacaaggc ggaggccgat ccggccgtgg ccgctgcatc cattctcgcg cgcgacggat
    29761 tccttcaggg cctgaaggcc ctgggggaaa aatacaaagt gaagattccc aagggcgcgt
    29821 ccgaccaggt tcgtgcggtg gcccgcgacc ttgttcagaa gcacgggccc gaggttctgg
    29881 ttcaggcctg caagtgccac ttcaagacgg ccgatgccgt gctggcggaa atgaacatga
    29941 accgcgccgc gctggggccg cttggacagg cggtttcaaa ggactatatc cgcaaaacaa
    30001 aagagggatg agcgcgctca tccctcttcc atctgcaaac cgaattaacc cttcagcagc
    30061 gcctgcttgc aggccgaaga cacgcggaat ttcacgacct tcttggccgg gatctgaatg
    30121 gtttcacccg tggcgggatt gcggcccatg cgggcggcgc gatccaccag caccagcttg
    30181 cccagtccga gaagcgcaaa accgttcttc gcttctttga tcgccaaagc acccagttca
    30241 tccagaacct gcgcaacgtc cttcttcgac aggccggttt tttcggccag cgcggcctga
    30301 atctgcgatt tcgtcattgc catcggaatg tcctccagtt cggtttgttg ttaatctgac
    30361 accaatctac ggcggcattt tgctccggcc gcatcataaa gcaagcgttt tttcagggat
    30421 tatgggggtt tccagcccgc cctcaggcct gctcgtccat cagtctcatc atctccagca
    30481 gcagggccat ggtgggacgg gtgatgctcg catccctgaa tttcggctca ccgggctcga
    30541 agctgaattc aacgccgccg cagcggccga gctgaagcac cgcatcttca cccgtaagac
    30601 tttcgcaccg ggccgccaca atctgcccat tcgcaaacgc gatgtctgcc accctgcggt
    30661 tgctgtcatc ggtcaaaacc atgcccccgc ttttctgggt ggaactgatc aactggatca
    30721 ggtcggggat cgacaaaata ttgagcaggc ccgaaaactt gctgttttcg tcccgcacac
    30781 gcttcatctg ggcctgggcc tccttgtaaa tgacatcctc cgcccttttg cggtcggtga
    30841 tgtcaatgaa agagaacacc atgcccatca ctttcgtccc gctgccgaat atcggactct
    30901 gcggaatgtt cggcgcaatc gacacctgca caaaaaacgg tttcccgccg ggccgcggca
    30961 cctccatctc cgtctggcgg gtggcgccgg acaggaccga ttcaatcatc tcgtctgttt
    31021 tcggcccggc cggaatgaaa tccctgatgt tcttcttgag cacctcgttt tctgaccgct
    31081 gcccccacag tcgcagaaag gtcgggttca catactgcag caaaccctcc ggcccggtga
    31141 tggccacgcc gctggcaaga ttctggatgg cgttgtgctc ggtccggagc atttcctcgg
    31201 tcttgacgcg gcgctcgatg ttggtgatcg aaaaaacaag ggcctcttca tccgccatct
    31261 ggatgcggct gacagaggtc tccccgggga aaatcgtgcc gtcctttctc acgcatttcc
    31321 cgttcagcac cgtgaatcgc cggtccttca aataacccag gattttctca atcttggtct
    31381 gggaaagccc cgggaacaca tcgcagacat tcatggcgag cagctcgtcg cgggaataat
    31441 gaaaatgctc ctcggcgcgg acgttggtat ccagaatacg gccgtcgctg cggcagataa
    31501 agacggcctc gtacaggccg cccagcagct cccgatagcg ggaatccgga gatttctgcc
    31561 ttaccacctt catatcactg acacttgatc aaataatagc gggccgtgtc cggaggtgaa
    31621 ccgacactct gccagaccgt cgtgctgatg ccggtggctt caaacagcgc cgacgctata
    31681 ttggtgaacg agtgccttcc cgcagacagg ccgccctgtg agtccgtcac atacagcacc
    31741 tgataccagt ttccggacac caccgaaaag gtgatgccga tctgatcggc gccgagcgaa
    31801 agcccggtga actgcgcagg ctcaacctcg cgaacctcat ccgcccacca gaacccgctc
    31861 tgaagcaccc tgccttccac gttcgtcgag agcgaggtga acccgtgctg agcaatggat
    31921 ccgctcagcc ggaccgtgcc ggagacgttg gtcgccatga ggattccgcc cgcattcagg
    31981 ctctcggctg tgaaggtgaa attcgtctgc tcgctcaggc gcgcggcctg cgcgcacgga
    32041 agcaaaagcg cccccgccag catcgcggcc cctgtgtgtt tcttcattct catca
//
LOCUS       DAOQWR010000011        31338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1705_length_31338_cov_5.190039, whole genome shotgun sequence.
ACCESSION   DAOQWR010000011 DAOQWR010000000
VERSION     DAOQWR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 31338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31338
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_31338_cov_5.190039"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="PKK36_01850"
     CDS             complement(<1..1435)
                     /locus_tag="PKK36_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93330.1"
                     /translation="MKKTAYRAGGVSGMECIVWTLLFSLFLVLPLHAETRYVWDSCPT
                     PQAPYTVWSNAAVNIQDAVDVSSPGDLIRVTNGTYSSAGAAAPGHTLTNRVCLTNGIT
                     VSSVNGPAVTSIQGAGPIGSDDSRRAAYVGTGSVLLGFTLTGGHTKNSSAPEIDQCGG
                     GVLLDGGLMSNCVVQACAADLNGGGAFIRGDGELYNSSFLVNTSAYGAGMYFSSGGYV
                     SKCVVISNAAVWGGGGAFFLDAGTLHDSDVMQNYASGGSGLYFLNGGTADDCRIFNHP
                     ANDWGGGVVFENTGGVLTNCFVYGNSSAYGGGIYFYYGGMADNCTVSNNSSSSDGGGA
                     YAESGGEIKNCLVITNYAANAGGGLYLYYGGTVLDSTISENTSFFGGGLCMENTGALL
                     SNSFVIANMGDYGGGLCLLYDAAVQRCHVEDNHASILGGGAYMYQGSEILNSRFLGNH
                     AESTGGGGIYTEVTGRVFNCTIIGNTTT"
     gene            1591..3576
                     /locus_tag="PKK36_01855"
     CDS             1591..3576
                     /locus_tag="PKK36_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93331.1"
                     /translation="MKKHVKQQDLQRGAARAGYVCLYLLLLAGFILLAYGQSLSHRFA
                     WDDYGLIVDNDQIKVASGWLRSFARNFWDLGENAKDPTRSFYRPVIVLSYVIDHAIWK
                     MNPFGYHLTNIVAHMLCSFTVFLVMLKLAGNPHYAAIGALVWAINPTHVENIAWISGR
                     TDIFSGVFYFPALYFCIRALETPRQRRTFFLLSALLYLAALMSKEMALSFMPVAALFC
                     FVYVKRPFSARVVAFPSLLYAIVTVGYLSARIAVLGQIAGPPVFGSLMERFMSIPMVF
                     AKYLGLLLNLVPVDPHHAEGFLHAGRAAAGVLYLLLATVFLILAVWAIRRGNRLTGFG
                     MLWFVITLLPVFNLGTFGDVLYADRFLYIPSFGLALIIPAIMTESRSRILSLRVVQMG
                     LGLWLLFYLISSLTLSRLHTAYWEDSTTLFTHAAGTSPNSAYIHYNLGYSLADKGRHE
                     EALAALQRAIILQPNYPQAYVRLGISLNALQQYEAALHYFRKALRYGYFSDVIYNHIG
                     ITYMNLEKLEQAKRFYGKALALRETAEYHNNLGECLLGLKEYKQAAKHFLKGLNMEPS
                     PAIYNNLAILAAEEENYSDACAYLERALQFPSEALTTDTALVIHYNLARFLSLQNKPE
                     EARLNAMAAKSLLNDAGNGKNSGSLILRELEKILESQ"
     gene            complement(3580..4509)
                     /locus_tag="PKK36_01860"
     CDS             complement(3580..4509)
                     /locus_tag="PKK36_01860"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HNR93332.1"
                     /translation="MKKAPPAVVFAYHNMGVIGLEALIRRGVDVKMVFTYHDDPCERC
                     WFASVADWCGERGIPCDRDAAANSALFVEMVRACEPDFLFSFYYRDVLSAEVLKCARR
                     SAINLHGSLLPKYRGRAPVNWAIINGEKETGATLHYMTARPDAGDIIGQRSVVIGEQD
                     TASDVYAKLELAAGILLDEMLPMILERNAPGIPQNEDEATVCRRRRPEDGLINWHDSA
                     RRIFNLIRAVTEPYPGAFTWLRGDKLIIWRALPEVESFGLAPGELGAADRRIRIGTMA
                     GSLLVEKAEYRGRILAGGELTDALLPFAGSFLS"
     gene            complement(4506..5399)
                     /locus_tag="PKK36_01865"
     CDS             complement(4506..5399)
                     /locus_tag="PKK36_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR93333.1"
                     /translation="MTPSLTLRIDVDFPQLAADTLPPMLEELERFGMKATFFIAAAAS
                     RPGSLLRRVRNGSYVRRILRMGTGRSLLKMLFTMNASRGPVPELPALVRRIEQAGHEI
                     AVHGYDHVWWADRVWTAPPEDLAGQIEKAYACFHSVTEAAGLAWASPGWRSTESAIRL
                     LQDRGVPYFSECWGRGPFLTQLDDGSVIRIPHLPITLPCMESLRTTECLGPEESVQKI
                     LNLLKSDGYNMLCAHDYFEGLLGRGYFTAFLEHCSARAISTETVKAIACRLHTDLPVC
                     RLGRGHIPGFEGQVSVQGDTA"
     gene            complement(5416..6138)
                     /locus_tag="PKK36_01870"
     CDS             complement(5416..6138)
                     /locus_tag="PKK36_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93334.1"
                     /translation="MNDSVTVLIPVYNEAPRIAGLLNGVLKTMTALGVPWKVIIINDG
                     STDWSSSLEHSLTANQNVAIRTMTPNRGKGAVLNDMFAEIETALTVVIDGDGEYSPRD
                     IPVVLQPLLNNEADWVLGSRFGFGRERPPQYMLTYLANRIINLAFRLMSGLRFRDILS
                     GLYAYRSALTRSVHLDNRRFSFTPELIWRVQRMHRPRWQEVPAGYYFRTYGEGKKIRW
                     WETFTILCSICKYRFKKLDQIP"
     gene            complement(6138..7343)
                     /locus_tag="PKK36_01875"
     CDS             complement(6138..7343)
                     /locus_tag="PKK36_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNR93335.1"
                     /translation="MAVKSQIPVMDGGRPVREVFLPFARPRITETDIQGILETLHSGW
                     LATGEKTARFEEAFGGYVGNRFAVGLTSGTAAIALALQVAGVRAGDEVITTPMTFAAT
                     AAPIVHAGAVPIFADIDPGSFNINPDQVAARITKKTKALLAVHFAGRPCEMGILRHLA
                     NSHDLVLISDAAHAIEARYDGRKLAEWSDISTFSFHPNKNITTGEGGMLTTNRQAWAE
                     HARRLRFHGIARLSDDGFPVTDVVEPGFKYNMSDLQASLGLTQMETIENGLESRRLIF
                     EHYNEAFRGMPGVSVPPPVPERSRHAMHMYNILLDTNCLCINRNRFCRALNLENIGAG
                     VHYRSLHQMSFYREHCQLKDEDYPASAFVSDRILTLPLFAGMSVDDAQSVVEAVAKVL
                     RCYRRNGVR"
     gene            complement(7352..9655)
                     /locus_tag="PKK36_01880"
     CDS             complement(7352..9655)
                     /locus_tag="PKK36_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR93336.1"
                     /translation="MNPRLHLQGKKATLLTAVLVLIILKIAAASFCRAGRTVHVQIRC
                     NGSQIVAVLNGGYEVSIIDNSEGRGMGFYAFLPEGSGRRPFYARNLVLTPAEGLFSGI
                     PQSVSLLASCEYGRRITISDGWRIVPGRGLTFQGAEGSRGTAMLKGLAFRDFDLEVDF
                     ADATDSGILLRAQDADNGIVFVVRPPLNDAFFFKLTNGVPGPILNLIPISQLNADREI
                     LELANLLLEIFLNAALLILLLKGLLWLIPRRELFQIRLRPGLCILRVLIPIILTVMTF
                     LVLAAVALIAFDGIPHIDDEQVYCFQAKIFAEGHFWAPVPAAKDFFKHWHIVMDGNRW
                     FGKYPPLFPAILAIGMKLNRPWIINSVLGAMTGLFIFLTAKKLRGYACGLTAWILALT
                     SPFYLIVGGSMMSHMAAALFTIMTVYFMLAVKETGGSGVFMSAGICMGLSFLTRPYTA
                     LLVSFPVLLFAAYEVDGWSSRRRAALALLAFAAGVAPLVVLSLCWNYIHLQGRGFPLG
                     LYELYDATDTLGFGDLRGAGQRLTWGTWGHTPAKGLRSIYTYLDHTSLFFLGWPCRLS
                     FAFVMYGLVGVCRDRRKLLLAGIFIALVAGHFLYWATEHVGIGARYWFEAMPGLYVLA
                     ACGIFRLTTCRRDRAGPSVAAAGITSHHVLVWAALLFFVFMNVTAYLPKKLKGLHNLN
                     NITARLKTIVSEMHLSNAVVFVQTSGLSENDGFFMNDPFMRSGPIFARDMGARNGEIL
                     AQYPGYEAYLWDHLKLRELDPEEVKDE"
     gene            9734..12085
                     /locus_tag="PKK36_01885"
     CDS             9734..12085
                     /locus_tag="PKK36_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR93337.1"
                     /translation="MQRSNQNPLRDISEGRRYGALPLLLVLLYVLIFLSDRHLLSNRL
                     VSLKLRCADSNYELIINGRSHQTKAGATASDGHRIGFYAWHRGEPETKQTFFRNLKVE
                     SLDGKGSELQLSLTDGQQLALLQQPACDTPPGLLSRDARWEIAKNEGLHSQAIAPGAA
                     LVLVDRFEAANFVMSADIANPYDAGFLFHADDQCNGEVIAVRPAYNDVILFSISKGMA
                     NPIKHIIEYCPLQMSRELLRLTCLICRIIFWAGVIWQINLLLASIFRRRNTFFRLPHP
                     AVRATPAKILTAMLIFAGALLVFAFIASAGFKKIPHITDEAAYLFQARIYAEGHLWAP
                     PPPVTGFFEHYHLMIEGQRWFSKYPPLFSLVLALGVLSGWPWLINPLLGAMLTVTLYL
                     IARQLFSFRIALIASILLIVSPLHMFMSATMMAHTLTALCLWQMIYQSLAGIQTGNPM
                     RFAAAGALWAAAFMTRPFTAAAVLLPASIGAAVSWRRCFTRKAAGKMLAASAAGAYPI
                     LALFACWSVLYCSDSGQPMIPYISYHAADTLGFGADKGAGWLMTWGSWGHTPAKALRS
                     ISVFLENTMQYFHGWPLGMSLVFVPLAFWDKANSKRPMLFLLGVFLSLVAGHAFYWAT
                     EHLGYGARYWYAGMPGITLLSAVGLNSLIRQSEIYGKDIRCTPSIFFPILLTGVFILW
                     NAFVYLPPKFFEARTYGGISSRLGDQVAAAGLTNAIVFVKTENSLYNDGFHLNDPLLR
                     KGPYFVNDLGARNDEMMQLYTDYHFFRWNKTSLLPIRTDLAAP"
     gene            complement(12090..12743)
                     /locus_tag="PKK36_01890"
     CDS             complement(12090..12743)
                     /locus_tag="PKK36_01890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR93338.1"
                     /translation="MRAALSGLGELQGMRGLVLASGLGCECVHLARRGATITGVDIAG
                     ERTELARELVRRHAPEVAIRFVTADAECLPFDDASFNFIFSRDVLMYGDWRKMMVECA
                     RVLQRGGRAVFMEALADHPAVKLYRRLGSDGAQRGFTRYLEYGEMGALSSTMRIVHIK
                     AFYFLSLAAFYFLLRCRSVQAFRISLCLLNPLDSVLFRLIPALQRRAWRAVAVYKKE"
     gene            complement(13106..14836)
                     /locus_tag="PKK36_01895"
     CDS             complement(13106..14836)
                     /locus_tag="PKK36_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR93339.1"
                     /translation="MPAAYPRILRREGGRHPKNLYAALLAVCASSLLHALLIRHCPSF
                     VLGRASDLALLKRAHAVVLDKVLPDYRPEQLQMERPVRPEDPGRWAEDIMEKTALSPL
                     RPAETAPSAWTHEPKAPVQSAAPASEWDARQQVVSIEERIYADEVSALPRRFADDVPR
                     SYDAADMRLQTPEEIPAAASLPPGGGYAADVALPVIFPAAGIGTGDEMHSVPAGLSGS
                     LLEEAPGEISEMEPVEDLLALNISVFRPRDEEWLYFQIGIGQQSADALPVLSKQLVFL
                     QDCSESMTAEKVEQCKEGLMQCVNRLAERDEFNIMAFREDTAACYPAPVAVTPLSQAR
                     ARWFINDMQSRGNTDVYAPLRQVLAQSVDATNPVVAVLITDGRPTSGMLDTSEIIDRF
                     TQSNQGRVSVFTVGGGQRVNRMLLDFLSYKNRGDAHVVMEPQKIPETMTELLREISRP
                     VLTGLDYQCAPLDEGDIYPSSLTHLYLDRPLMLYGRCHPDTEDVLLQITGTALGHGYD
                     MIFPLRWDGAGDGGSALRSQWVWHRIYDLMGRYIRDGNQDVLEEMQSWASRYGLVIPY
                     TFTSEEERTY"
     gene            complement(14841..16073)
                     /gene="rho"
                     /locus_tag="PKK36_01900"
     CDS             complement(14841..16073)
                     /gene="rho"
                     /locus_tag="PKK36_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNR93340.1"
                     /translation="MQRQTVPELLALADELKLESLGALRKHELTFEILKAHARCGGNV
                     IGKGILEILPDNFGFLRSPIYNYLPCPEDIYVSPSQIRRFGLRTGDLVLGEIRPPKDK
                     ERFFAMLKVEKINNDDPEKTRRVVPFENLTPLFPDERLVLEVDPAEISMRVMDLMTPV
                     GKGQRGLIVAPPRTGKTVLMQKMANSISKNNPEVKLVILLIDERPEEVTDMIRNTKAE
                     VLSSTFDEPPERHVQVAEIVIEMAKRMVECGKDVVILLDSITRLARAYNTLQPHSGKI
                     LSGGVDANALHKPKRFFGAARNIENGGSLTIIATALVDTGSRMDEVIFEEFKGTGNME
                     LGLDRNLVDKRIFPSINVEKSGTRKEELLLHEDELAKIWILRKALNGVPAVEAMELLI
                     GRLKKTRSNAEFLITLQG"
     gene            complement(16328..16957)
                     /gene="coaE"
                     /locus_tag="PKK36_01905"
     CDS             complement(16328..16957)
                     /gene="coaE"
                     /locus_tag="PKK36_01905"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNR93341.1"
                     /translation="MTRIAARSPVLLGLTGGIGCGKSAAGAALESLNVAVMDADIEAH
                     RLMRRGMPVYDEVVQAFGPAIVGTSGEIDRGRLAEIVFRDRARLELLNTLVHPAVREC
                     WRQWTAARFEEGRDAAVIIPLLYEVGETAGWDAVICVAASEALVRARLRERGMSEQQM
                     TERIGAQMPLDEKCRRADYVIWNNGSLKELKESISVVYREIISKRERSL"
     gene            complement(16954..18942)
                     /locus_tag="PKK36_01910"
     CDS             complement(16954..18942)
                     /locus_tag="PKK36_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93342.1"
                     /translation="MKYRNILFVLGLVVLYALGVGIRREVLQAQYRALGESPAFTLES
                     ALHFRMIQTAMAGRLPEMDFSVEYPSGVNVRRTYSIGSEYFYALTARCWPSRMPLAER
                     IRWMEVLWFCLGIPLMVLWIRQAASSPAAGWISGFFYAVAISSVIRSTGQEISRENFA
                     LPFLIAHLAFSARAVCGFPRARLWAFSSALCLSLAAVNWDLVQFYVFLWAVAGAVRLL
                     KGGFVSRPAFRELWLFEWTALMFFSLLNPYMRAHGWMISPPMLLACGVTAACILEQRP
                     GVHLLRRHYLLLLALPLLLGIVIHTAVSGSYAHFGELLWAKIRFLNHKPADPSLLSFS
                     QRIMWVPALHSTTWAQARLLFPGLLGLTLGAFCVCCFYQLRRAQSELFQFVFFYGASL
                     ITFWFFFRFHVYLAVFASALPGVLYGKLKERTPALRWAFVLLAGCVGVVEARNTLAQP
                     EQWGRTNVYYRELDELTEWFAKNGEQKPVAANFGVSASILAYGGSPIVLHPKFEGVEI
                     RRRVERYGEALFKGDESEFRAWAGQYGAEWYVHALGEFSSRSPELQMRYFVNALNPPA
                     EAAARMFEFRPEEGRWFRYRWGNRKYRVFEIISEADEEMAARSAARAEEELQHGNLDN
                     AEYYAVEALKADDRHTGAMNVLKHVGVLREQNFMSGHL"
     gene            complement(19020..19892)
                     /locus_tag="PKK36_01915"
     CDS             complement(19020..19892)
                     /locus_tag="PKK36_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNR93343.1"
                     /translation="MTNAKSLLLLDGLAMAYRGFYAIPDLTTKDGRHTNALMGFIRMM
                     LQLKSAWKPTHWAAAFDGGRPEERMAALETYKAQRAPMPDPLKEQIPYINEYLEAAGV
                     PSFRLDRQEADDVMATLAVRAAEEGASVYMATSDKDMYQIVSDRICVVSPAKEMVCMG
                     PEEVVTKTGVRPDQMVDYQALIGDAVDNINGLPGVGPKTAAKWLKEYGTLDEILKHAA
                     EIQPARFRGVLAQHEPTVRRNTGLMKLRTDLNVSLNWEDLAVREGDRSRLVSFFSKYE
                     LHSLSRMVQGELGL"
     gene            complement(19984..23055)
                     /locus_tag="PKK36_01920"
     CDS             complement(19984..23055)
                     /locus_tag="PKK36_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93344.1"
                     /translation="MKLARAIVAIMLCMWLNPAVQVSGQDDLNIDMLLEDLTGDPSVA
                     PAVVAEDAAAELPADEEVTAEDEFPMGEESPAVEEAAAEEMPSFESEDLPAMDEPMDT
                     PEMVVTDDVDLDAFLSEEPVMDIPEELPAEEAAPVQDDLMFEAPVEEDVVPSVEMELE
                     PVFEEEEASAVEDLFSSMGEEPLPDETMDMPVEEEVMTDEVDAEAMPEEKAPSDEPVV
                     DKKTAKLRAQQEEVRRQADETSALQAIDSGFKALDQSDFAAAVKYFEDALSKLPPRPA
                     TQQNRDHAIWGLVEASYRDARNMARDPARLEAAMKSVERALKLDPSHGPSARLKTRLA
                     RDIERQAAIAARPVPLEDRPVVIEKKKTAEELVAEGRQYFDMGDYKSAEALFDQVLVV
                     DEFNTDAMRYLRRIEEHWYRVSTLERKATVAESMRKVREKWNPPTGEVELPETVVKQE
                     SIDTKTTTEKLREKMSSIMIPSIEFRQANIRDVVNFLVDASLAGDPEGVGVNIILNLN
                     QPGEAAPAPAPAPAAPADPFADFPGFDTGMMEQPSDFGGGGGGSSIPTITLNLRRISL
                     IDALKIITEVSNLRYRIDGNVVIITPAGVATGVIITRMYPVQPSIIDVIVERGSEQTE
                     KTGEFVEMTGSSVTMTRGDVKKFFTDAGVPFPAGTSITYNPSISQMIVANTAENLEIF
                     ERILAQLNVIPSQVEIEARFVEIGQNDLEELGLEWILTDNYEIARKKGNAPIAGQERI
                     QADANPSGLTQGLRFFGFDGNTGAMAPASAITRGTSTAMGNILSFSSVLTNPELQMVI
                     HALQQKGGSDLLSAPRITTRSGVNAQIQVVQEIIYPTVFESEVITIDREVAGGGTIQE
                     RLVTVTPGSFETRETGVILNVTPTVGPDGYTIDLTLVPEVSELVDWIQYGSVIDRDED
                     GNPIGYNIPQPVFSSRNVTTSIVVWDGATVVMGGLIREEMVTMEDKIPVLGDIPLLGA
                     LFRSKGEMSQKRNLLIFVTARLVDPAGKPIHKADMMAMPGGSTAVDETAMK"
     gene            complement(23164..24252)
                     /locus_tag="PKK36_01925"
     CDS             complement(23164..24252)
                     /locus_tag="PKK36_01925"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNR93345.1"
                     /translation="MSAAPREKTSWLATSYEKLILDVALIVLAALCILLALNLIGGDA
                     ASSVRGSGVAQQEGITATEMDPAVWVEKDAAMRSPFQMTIQSNLMLVSQLRVYCVNPS
                     CEKPIAFNAAKCPFCQAAQPSREDIAGMDYDGDGMPDQFEKKFGLNPYDPGDSALDLD
                     GDGFSNVEEFQNKTDPTDPEDHPSPAAKLRMVRMVVKPFFLRFQGVSELATGKSFLLN
                     LRSADKSHFAKLGAVVEGYKVAAYDEASSILTLEKDGKSLQLKKNEPITAKEFIAALV
                     SLLDGRQYQVRVNDAIDVKGRPHKVIDINPSHVLIEDEQTQKQIQVPPLTEEERMILR
                     GISEPSAMQAEPGAVESAAAQFPPPAEP"
     gene            complement(24249..25313)
                     /locus_tag="PKK36_01930"
     CDS             complement(24249..25313)
                     /locus_tag="PKK36_01930"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HNR93346.1"
                     /translation="MNLRKDKLGLIIGAAVIAVVAIVLAAVLIKVWQGYSTVKSDLDG
                     KKARLTQLQRRDPYPSRENVERIAENANLMEQRFEALMGDLLQGQIEPKPMERAEFPP
                     LLERSVRRLRSAAEEAKVTLPAGFSMGFDRYLKGELPQNTNEISRLIVQLQEAEKVLG
                     LVFSVGLGEIVSFNRELFEGTASAAAEIDASDPRAARRARRAARTEDAAEASGPVALK
                     KQSELYSSEHFVLEVRGREEAIWRFLNEMARNDMKIVVTSIQLSSDQPDIKRAVEAIK
                     QASKTAADLAARGAMSGQSQRTGMFQQGRVPTLAGTAAPAIVPGAEERVVAGREPVTA
                     KIEFDIYRFKTQTSTQETAE"
     gene            complement(25310..26983)
                     /gene="pilM"
                     /locus_tag="PKK36_01935"
     CDS             complement(25310..26983)
                     /gene="pilM"
                     /locus_tag="PKK36_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNR93347.1"
                     /translation="MLNKDRILALDIGASSLKMAEFVALKSGGLELINFSCESLGVDS
                     QSDVDRGAFIVQAVRRMMRETGIKPGPALVSVAGQSVFSRYVKLPPVDSERIYQIILY
                     EAQQNVPFPIDEVVWDYQLIGGATGEVDVLLAAIKAEMIEKMTDSVGEAGLDPDLVDV
                     APMALYNTVRYNYDDLAGCTLVVDIGARSTDLIFIEEGRIFTRSLPVAGNTITQHIMR
                     EFNMNYADAEDLKRAHAFVAFGGAYEGPASEVADKVSKSVRSIMTRMHAEINRSINFY
                     RSQQGGGKPELILLTGGTSVIPYTDTFLKEKLKVEVDYLNPFRNVAVGASISAEEIGK
                     SAHLMGELVGLGLRKVLSCPIELNLMPKKVLKEKELRKKRPYFIGAGVAAVLAMMAVT
                     GYIARVKSYAASQLADISARVDDLAAIESQLSAEEQRIGDISGKMDEIYGLMNQRSLW
                     IRMMSQIHSALPSGMWIASIVPVVDSGPAAPAGQPKAPGVVTALAVKGRGYLDVVRSA
                     TPILEFRDNLRKLDFVSEETEIQRQPAPGADDFAREFEIKIVLQEGLPL"
     gene            complement(27004..27591)
                     /locus_tag="PKK36_01940"
     CDS             complement(27004..27591)
                     /locus_tag="PKK36_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93348.1"
                     /translation="MKKTQLILAACLAALLGFISQTASAQAGAQDVARIRKVSGSKVK
                     TPDFKVSGFNTSGAAKDWFAVMVEYDTAPEWTEQLDFTFYVVLQGKDPRYQLLRGTVS
                     CVNVPRGSRHKEVMYLHPSTLGRYGTVYRAGVEIKHKGVSVAVESSDQGKGRWWEDPN
                     LLPGIRDGLVLNRLQTPFAFLQYDEYEAINPAPAK"
     gene            complement(27734..28546)
                     /gene="lpxA"
                     /locus_tag="PKK36_01945"
     CDS             complement(27734..28546)
                     /gene="lpxA"
                     /locus_tag="PKK36_01945"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNR93349.1"
                     /translation="MASSDIHMTAVVEPGAELAAGVSVGPFAYVRAGSKIGPGTEIGP
                     HAVIHEFTEIGENCRIHAHAVLGDLPQDLGFKGVKSFVRIGSSCTIREGVTVHRGTME
                     GSATVMGDNCYLMAYSHLAHNVRLGSRVILANNVLLAGHVEVGDGVFIGGSAAVHQFC
                     RIGRLAMVGGLSAVSKDIPPFCTHASGAFNSIAGLNVVGLRRNGFDGTARAEIRSAFK
                     TLYASGLNVSQAVEKIRAAYSSSAVLEMCEFIANSKRGICAFRGSLRDAAAE"
     gene            complement(28546..29283)
                     /gene="radC"
                     /locus_tag="PKK36_01950"
     CDS             complement(28546..29283)
                     /gene="radC"
                     /locus_tag="PKK36_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR93350.1"
                     /translation="MKKDNLVYSIPVKQLPVREQPRELFDCYGAENVSDSTLLAILLR
                     KGIPGKNVTVLADELMQRFGSLSNMAKQSVQDLQAVKGVGKVGAQVLKCALELAVRMG
                     RETARPREKILTPAQAADQVRLQARTLNREVFWVLMLNIRNELKGPPVEVSRGLLDAS
                     LVHARDVFKQAIQSGSQAVVLLHNHPSGDPSPSKEDVALTRQLVEAGKIIGIRVLDHV
                     IVGHRKTETEQDYISLRETGLVEFERD"
     gene            complement(29270..30589)
                     /gene="hflX"
                     /locus_tag="PKK36_01955"
     CDS             complement(29270..30589)
                     /gene="hflX"
                     /locus_tag="PKK36_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNR93351.1"
                     /translation="MMQTEINKEKALLAGVAIGQARDWEIRDSLDELSRLVETDGAEV
                     VHTLICRRPKPDPGYYIGRGKAEEIRSLAELHGARCVVFDEDLSPAQARNLEELIGLP
                     VLDRTQIILDIFSMHADTHEGRLQVDLARLEYQLPRLRRMWTHLERQVGGIGVRGGPG
                     EQQMEVDRRRIRQQIERLKDQLEGVRRHRSELRRGRRRHGWASVSIVGYTNAGKSTLL
                     NALTGSSVVTNDQLFVTLDPTTRRVELPNQQMALLTDTVGFISKLPHHLVEAFKATLE
                     EVVDADLLIHVIDASHPLAERQIGVVNDVLRDMGTDDKPVVAVLNKIDCESGLAQARR
                     LSKLLPGSVMVSARTGEGLDHLKEQLADTLRHRHTEVELCIPLDQTRLIAGLRANARM
                     ADEKYEEDGFHVRAWVPERILHLYRPYIRGEPPHAAAPDDWSEDEKG"
     gene            complement(30593..>31338)
                     /locus_tag="PKK36_01960"
     CDS             complement(30593..>31338)
                     /locus_tag="PKK36_01960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNR93352.1"
                     /translation="AVSYLGIDLADPSDTFSAGAWLEHVRVSLPDAPVIVAGGTGLYI
                     KALLQGLDAGAPPDPALRAELESLAAERGLEALRQKLLNEHPDWYASVRDRENPRRLI
                     RAIEIAHSGAPPPKAELPPVRITGLYMERGALEKRIARRVDLMYQNGLMEEVRRLREQ
                     HPRFSETAAQAIGYAEAAAVLSGKMSLAEAKERTVLRTRQLAKRQMTWFRHQAEVEWI
                     EVKPGMTVGETAEQVCAAWAKQDPVRLNV"
BASE COUNT         6861 a         9577 c         8150 g         6750 t
ORIGIN      
        1 tggtggttgt attcccgatg atggtgcagt tgaacacccg gcctgtcacc tctgtataaa
       61 tcccgccgcc gcccgtactc tcggcatggt tccccagaaa acgagagttc aatatttcac
      121 ttccctgata catatatgcg ccgccgccca gaatcgatgc atgattgtct tcaacatggc
      181 atctctgaac cgccgcatcg tacaacagac agagcccacc tccgtagtcg cccatgtttg
      241 caattacaaa actgttactg agcaaagccc cggtattctc catacaaagc ccgcctccaa
      301 aaaagctggt attttcactt attgtgctgt ccagcacagt cccgccataa taaagatata
      361 agcccccgcc ggcatttgca gcgtaattgg tgatcaccaa gcagtttttg atctccccgc
      421 cgctctcagc atacgcccct cctccgtcag aagaagacga gttgtttgaa accgtgcagt
      481 tgtcagccat gccgccgtag taaaaatata ttccgccgcc gtaggcggaa ctgttgccgt
      541 aaacgaagca gttggtgagc acccccccgg tgttttcaaa gacaactccg ccgccccagt
      601 catttgccgg gtggttgaaa atccggcagt catccgcggt gcccccgttc agaaagtaga
      661 gtccgctccc tccggacgcg tagttctgca tgacatcact gtcatgcaaa gttccggcgt
      721 ccaggaaaaa ggccccgccg ccgccccaga cggccgcatt gctgatgacc acgcatttgg
      781 aaacatatcc gccgctggag aaatacatcc cggcgccgta tgcggacgtg ttcaccagaa
      841 agctgctgtt gtacagttct ccgtccccgc gtatgaaagc gcctccgcca ttcagatcgg
      901 ccgcgcaggc ctgcacaacg cagttgctca tcaggccgcc atccagcagc acgccgccgc
      961 cgcactgatc aatttccggg gccgatgaat ttttcgtatg tccaccggtg agggtgaaac
     1021 cgagcagcac actcccggtg cccacatagg ctgcgcgccg gctgtcgtcg cttccgatgg
     1081 gccccgcgcc ctgaatagat gtcacggcag gcccgttcac actcgaaaca gtgatcccgt
     1141 tggtaaggca gacgcggttg gtcagggtgt gcccgggtgc ggcggccccg gcggaactgt
     1201 atgtcccgtt ggtgacccgg atcagatcgc ccggcgatga aacatcaacg gcgtcctgta
     1261 tgttcaccgc cgcgttggac cacacggtat aaggcgcctg gggtgtcggg cagctgtccc
     1321 atacatagcg ggtttccgca tgaagcggca aaacaagaaa caagctgaaa agaagagtcc
     1381 atacgatgca ttccatgcca gagaccccgc ctgcgcgata tgcagttttt ttcataatcc
     1441 aaccgccttc ccaaaagtcc tgcccaaagg tgtcatactg tgaaacatac cataatcccg
     1501 cctaaaatat aggcggattt ctgcacaaaa tacacatttg cattgttgtc accacgcgcg
     1561 ttgcctatat tgtcgtaaaa cccggcattc atgaagaaac acgtcaaaca gcaggatctt
     1621 cagagaggcg ctgcccgtgc cggatacgtc tgtctttatc ttcttcttct ggcgggattt
     1681 attctgctgg cgtacgggca gagtctttca caccggttcg cgtgggatga ttacggcctg
     1741 atcgtggaca atgatcagat caaggtcgct tccggatggc tccgctcttt tgcgaggaac
     1801 ttctgggacc ttggagagaa cgccaaggac cccacccgct cattctaccg gccggtcatt
     1861 gtgctgtctt atgtgatcga ccacgcaata tggaagatga acccctttgg atatcatctt
     1921 accaatatcg ttgcgcacat gttgtgttct tttacggtct tcctcgtcat gctgaaactg
     1981 gccggaaatc cgcattatgc cgccatcggt gcgctggtat gggccataaa tccaacgcat
     2041 gttgaaaata ttgcatggat atccggccgc acggatattt tttccggagt tttctatttt
     2101 ccggccttgt atttctgtat tcgggcactt gaaacaccac ggcagcggcg caccttcttt
     2161 ctcttgagcg ctctgctata tctcgcggcg ctgatgagca aggaaatggc actgtcattt
     2221 atgccggtcg ctgccctttt ctgttttgtt tacgtgaaac gcccgttctc tgcgcgcgtc
     2281 gtcgcctttc catccctgct gtacgccatc gtcacggtcg gatacctgtc cgcccgcata
     2341 gccgtgctgg gccagattgc cggaccgccg gttttcggaa gccttatgga gcgatttatg
     2401 tccatcccaa tggtgttcgc aaaatatctg ggactgcttc tgaatctggt gccggtggat
     2461 cctcatcatg cggagggttt cctccatgcc ggccgcgcgg cggcaggcgt gctttatttg
     2521 ctgctggcga ccgtctttct gattcttgcg gtctgggcca tacgacgagg gaaccggtta
     2581 accgggtttg gcatgctctg gtttgtgatt acccttcttc cagtcttcaa tctgggcaca
     2641 ttcggcgatg tactctatgc agaccggttt ctctatattc catctttcgg cctggccctc
     2701 attataccgg caataatgac cgaaagccgt tcacggatac tctccctgcg tgttgtacag
     2761 atgggtctgg gactatggct gctgttctac ttaatcagtt ccctaaccct gtcgcgtctg
     2821 catacggcgt actgggaaga cagcaccacc ctgttcaccc atgcggcagg gacttcgccg
     2881 aattctgcct atatccatta caacctcggc tacagccttg cggacaaagg acggcatgag
     2941 gaggcgctcg ccgcattgca gcgggccatc attctccagc cgaattatcc gcaggcctat
     3001 gtccgcctcg gcatttccct gaacgccctg cagcagtacg aggcggctct gcactatttc
     3061 agaaaagctc taaggtacgg gtatttcagt gatgtgatct acaatcacat aggtataacc
     3121 tatatgaatc tggagaaact cgaacaggcc aaacgtttct acggcaaagc ccttgctcta
     3181 cgcgagaccg ccgaatatca caacaatctc ggagaatgtc tgctggggct caaagagtat
     3241 aagcaggccg caaagcattt tctcaaaggc ctgaatatgg aaccctcacc ggccatatac
     3301 aacaatttag ctattctggc ggcggaggaa gaaaattaca gcgatgcctg cgcctacctt
     3361 gaaagagcac ttcaattccc cagtgaagcg ttaacgacgg acacagctct ggtgatccac
     3421 tacaatctgg cacgtttcct ttcgctgcag aacaagcccg aagaggcccg cctaaatgca
     3481 atggccgcga aatccctcct aaatgatgcg ggtaatggta aaaattccgg cagcctcatt
     3541 ctgagggaac tggaaaagat tcttgaatca caataagttt caagaaagaa aagacccggc
     3601 aaagggaagc aatgcatccg tcagttcacc tccggccagt atcctgccgc ggtactccgc
     3661 tttctcaacc agcagacttc cggccattgt tccaatgcgg atccgtctgt cggcggcacc
     3721 caattcaccc ggagccagtc cgaaactttc cacttcaggc agagcccgcc aaataatcag
     3781 cttgtctccc cgaagccaag tgaatgcccc gggataaggc tccgtaaccg cacgtatgag
     3841 attgaaaatg cggcgggcgc tgtcatgcca gttgatcagg ccgtcttcgg gccggcgacg
     3901 gcggcagact gtggcctcat cctcgttttg aggaattccg ggagcatttc tctcaagaat
     3961 catcggcagc atttcatcca gcagaatccc cgccgcgagc tccagtttcg catacacatc
     4021 agaggctgta tcctgctcgc ctattacaac ggaccgctgc ccgatgatat cgcccgcatc
     4081 gggccgggcg gtcatgtagt gcagcgtcgc gccggtttcc ttttcgccgt tgataatggc
     4141 ccagtttaca ggagcccgcc cgcggtactt cggaagcaga gatccatgga gattgatcgc
     4201 agacctccgt gcgcatttca gcacttccgc actcagcaca tctctgtaat aaaatgaaaa
     4261 cagaaagtct ggttcacacg cacggaccat ctccacaaac aatgcagaat ttgccgccgc
     4321 gtcacggtcg catggaatcc ccctctctcc gcaccaatcc gccaccgatg caaaccagca
     4381 gcgctcgcag ggatcatcgt ggtatgtgaa aaccattttg acatcaaccc cgcgacggat
     4441 caaagcttca agcccaatca cgcccatgtt gtgataagcg aaaaccacgg ctggcggagc
     4501 cttcttcatg cggtatcccc ttgaaccgaa acctgtcctt caaatccggg aatgtgcccc
     4561 cgcccgagcc gacagacggg aaggtccgta tgaagccgac acgcgatcgc tttaacggtt
     4621 tcggttgaga tggcgcgcgc actgcaatgc tcaagaaatg cggtaaaata tccgcggccc
     4681 agcagccctt cgaagtaatc atgcgcgcac aacatgttgt atccgtcgga ctttaagaga
     4741 ttcagaatct tctgcaccga ctcttccggg ccaaggcact ccgttgtgcg aaggctctcc
     4801 atgcatggaa gagtgatcgg gagatgcggt atgcggataa cggaaccatc atcaagctgt
     4861 gtcaggaaag gcccgcggcc ccagcactcc gagaaatatg gcactcccct gtcctgcagc
     4921 agccttatcg cagattccgt cgaacgccag cccggagatg cccatgcaag gccggctgcc
     4981 tccgtcacag aatgaaaaca cgcatacgcc ttttcgattt gccctgcgag gtcttccggc
     5041 ggggccgtcc agacccggtc tgcccaccag acgtggtcat aaccgtggac cgcaatttca
     5101 tgccccgcct gctcaatccg ccggaccagc gcaggcagct ccggcacggg cccccgagat
     5161 gcattcatag tgaagagcat tttcagcagc gagcgtccgg ttcccatccg caggattctt
     5221 cggacatagg aaccgttccg caccctccgc aaaagactcc ccggacggct tgccgccgcg
     5281 gcaatgaaga aggtggcttt catgccaaac cgctccagtt cctccagcat tggcgggagg
     5341 gtatcagccg ccagctgcgg aaaatcaaca tctatcctta atgtcagcga aggcgtcatt
     5401 tcacagcgtc cggtttcaag ggatctgatc cagtttcttg aacctgtatt tgcagatgct
     5461 gcacagaatg gtgaaggtct cccaccaccg gattttcttc ccctccccgt aggtgcggaa
     5521 ataatagcct gccggaacct cctgccagcg cgggcgatgc atccgctgta cgcgccatat
     5581 cagttccgga gtaaatgaaa agcgcctatt gtccaaatga acagatcggg tcaatgcgga
     5641 gcgataggca taaagaccgc ttaagatatc ccggaagcga aggccggaca tcagacggaa
     5701 cgcaaggtta atgattctat ttgccagata ggtcagcata tactggggag gtcgttcccg
     5761 cccgaagcca aaacgcgatc ccagcaccca gtcggcttca ttattcagca acggctggag
     5821 aacaaccggg atatcgcgag gggaatattc accgtcaccg tcaatgacaa cggtcaacgc
     5881 ggtttcaatt tcagcgaaca tgtcgttcag caccgccccc tttccccggt tgggagtcat
     5941 ggtccggatt gcaacgtttt gattggccgt caatgaatgc tccagcgaag acgaccagtc
     6001 tgtactgcca tcgtttatga taatcacttt ccagggaaca cccagggccg tcattgtctt
     6061 cagtacgcca ttaagcaaac ccgctatacg cggggcctcg ttgtagacag ggatgagcac
     6121 agtgacagaa tcattcatta ccgcacccca tttctgcgat aacagcgcag aacctttgcg
     6181 acagcctcca ccacggattg cgcatcgtcc acgctcattc cggcaaaaag cggcagcgtc
     6241 agtattctat ccgaaacaaa agcgctcgcc ggataatcct catccttcag ctgacagtgt
     6301 tcgcgataaa aactcatctg atgcagagac cgataatgta ctcccgcgcc gatgttttcc
     6361 aggttcagcg cacggcaaaa gcggtttctg tttatgcaaa ggcagttcgt atccagcaga
     6421 atgttataca tgtgcatggc gtgacgggat cgctcaggaa ccggcggagg aacagacaca
     6481 cccggcatcc cgcgaaaagc ctcattatag tgctcaaaga tcaggcgacg actctccaag
     6541 ccgttctcta tggtttccat ctgcgtcagc cccagcgaag cctgcagatc gctcatgttg
     6601 tatttaaacc ccggttcgac cacatcagtg accgggaatc cgtcatccga cagccgtgca
     6661 atgccatgga aacgaagcct gcgagcatgc tccgcccaag cctgccggtt agtcgtgagc
     6721 atccctccct ctcccgtcgt gatgttcttg ttgggatgga agctgaatgt gctgatatcc
     6781 gaccattccg caagtttccg cccgtcatat cgggcttcaa tagcgtgcgc agcatcactg
     6841 atcagcacca gatcatggct attcgccaag tgccttagaa tccccatttc gcaagggcgg
     6901 cccgcaaagt gaactgccag cagtgccttt gttttcttcg taatgcgggc cgcaacctga
     6961 tcgggattta tattgaaact acccgggtct atatcggcga atatcggcac agcaccggca
     7021 tgcacaatgg gggcagcagt cgccgcaaaa gtcatcgggg ttgtgatcac ttcatcaccg
     7081 gcccggacac cggccacttg gagggccagc gcaatggcag cggttcccga ggtcaaacca
     7141 accgcaaagc ggttgccgac atagccgcca aacgcctcct cgaatctggc ggtcttctca
     7201 ccggtcgcca gccagccgga gtgcagagtt tccaaaatcc cctgaatgtc ggtctctgtg
     7261 atccgcgggc gggcaaacgg aaggaagact tcgcgcactg gccggccgcc atccataacc
     7321 gggatttgcg acttaaccgc cattgtgatt cctattcatc tttcacctcc tcaggatcca
     7381 actccctgag cttgagatgg tcccataaat atgcctcata tccgggatat tgtgcaagaa
     7441 tctctccgtt gcgagcgccc atatctctcg caaatatcgg gcctgaccgc ataaaaggat
     7501 cattcataaa aaaaccgtca ttttcgctca gtccgctggt ttggacaaaa acaaccgcat
     7561 tactcaagtg catttcgctg acgatcgtct taagccgcgc ggtaatattg ttcagattgt
     7621 gaagaccctt cagttttttc ggtaaatatg ccgtcacatt catgaataca aaaaacagca
     7681 gcgccgccca taccagcaca tgatgcgagg tgatccctgc tgcggcaaca gaggggcctg
     7741 cacgatcccg ccggcaggtt gtcagccgga aaatgccgca agccgcgagc acatataatc
     7801 ccggcatagc ctcaaaccag tagcgggcgc cgataccaac atgttctgtc gcccagtaca
     7861 ggaaatgtcc cgccaccaga gcaataaata tacctgccag cagcaacttc cggcgatccc
     7921 tgcaaacgcc aaccaggccg tacataacaa aagcgaatga aaggcggcac ggccaaccaa
     7981 ggaaaaaaag agatgtatga tccagatagg tatatatgct gcgcaaaccc ttggccggcg
     8041 tgtgccccca agtgccccat gtgagtcgtt gtccggcgcc tctgagatcg ccaaagccca
     8101 aagtgtcggt ggcgtcatac agctcataaa gccccagcgg gaaccctctc ccctgcaagt
     8161 ggatataatt ccagcaaagg ctcagaacaa ccagcggagc tacccccgcc gcaaaggcca
     8221 gcagcgccag cgcggcacgg cggcgggacg accatccatc cacttcgtaa gctgcgaaca
     8281 gtaaaacggg aaaagaaacc agcagagcag tatagggcct ggtcagaaag gacagcccca
     8341 tgcagattcc tgctgacata aagacgcccg aacccccagt ttccttcaca gccagcataa
     8401 aatagaccgt catgatggtg aacaacgccg ccgccatatg actcatcatg ctcccgccaa
     8461 caatcaaata gaagggcgaa gtcagagcca gaatccaggc cgtcaaaccg catgcataac
     8521 cgcgaagttt ctttgccgta aggaaaatga acagcccggt catggcaccc agaactgaat
     8581 ttattatcca cgggcggttc aacttcatac caatcgcaag aatcgcgggg aaaagcggag
     8641 gatatttgcc aaaccatcgg ttcccatcca taacaatgtg ccagtgtttg aaaaaatcct
     8701 ttgccgcagg cacaggcgcc cagaaatgcc cctccgcaaa tatctttgcc tggaaacagt
     8761 agacctgctc atcatcaatg tgagggattc catcaaaggc tatcagcgca accgccgcaa
     8821 gaactaaaaa cgtcatcacc gtcagtatga ttggaatcag caccctcaag atgcacaggc
     8881 ccggccgcaa acgtatttgg aacaactccc tgcgtggaat cagccacaac aggcccttta
     8941 aaagcagtat aagaagcgca gcattcagaa atatttcaag cagaagattt gccagttcaa
     9001 gtatttcgcg gtccgcgttc agttgagaaa tgggtataag attgagaata ggtcccggaa
     9061 caccattggt cagtttaaaa aagaaggcat catttaaggg cggacgaacc acaaaaacaa
     9121 tcccgttatc ggcatcctgc gctcttaaaa ggatgccgga atcagtcgca tcagcaaaat
     9181 ccacctccag atcaaaatcc ctaaaggcca gccctttcag cattgccgtt ccgcgagaac
     9241 cctcagcccc ctgaaatgtc agcccccgtc ccggcacgat cctccagcca tcgctgatgg
     9301 taattctgcg accgtattca caggacgcca acagagagac tgattgggga attccgctga
     9361 aaagaccttc agcaggggtc aggaccagat tacgggcata gaacggccgc cgaccgcttc
     9421 cctcgggcag aaaagcataa aatcccattc cccgtccttc actgttgtca ataatggaaa
     9481 cctcataccc cccattcagc acagcgacaa tctgcgatcc gttacagcga atctgcacat
     9541 gcacggtgcg gccggcacgg caaaaactgg ctgctgcaat cttaagaatg atgagcacca
     9601 gcacggccgt cagcagtgtc gccttcttcc cctgcaaatg aagtctggga ttcattaaac
     9661 taatctagac aatctattta cggaaagcca gtctttgcct tatgatgcca cctaagcgcg
     9721 gttgtgtggc aaaatgcaaa gaagcaatca gaacccgttg cgggacattt ccgaagggag
     9781 gagatatgga gcgctaccac tgctcctggt actgctctac gttctgatct tcctgtcgga
     9841 ccgccacctt ctgtccaaca gactggtcag tttgaaactc cgctgcgcag acagcaacta
     9901 tgagttgatc ataaacggcc gctcgcacca gacaaaagca ggcgccacgg cttccgacgg
     9961 ccataggatc ggcttctatg cctggcatcg aggcgaacct gagaccaaac agacattttt
    10021 ccggaatctg aaggttgagt cactggacgg taaagggagc gaactgcagt tgtcactaac
    10081 cgatggccag caactggcgt tgcttcaaca gcctgcctgc gacacaccac ccggactgtt
    10141 aagccgcgat gcacggtggg agattgcgaa aaatgaaggg cttcacagcc aagccatcgc
    10201 ccccggggca gcccttgttc ttgtggatcg ttttgaagca gcgaactttg tcatgagcgc
    10261 ggatatcgcc aacccttatg atgcaggatt tctatttcat gctgatgacc agtgtaacgg
    10321 cgaagtaatt gcggtgcgtc cagcctataa cgatgtgatt cttttctcca tcagcaaagg
    10381 catggccaac ccgattaagc atatcattga atactgcccc ctgcaaatga gccgggaact
    10441 gctgaggctg acctgcctga tatgcaggat tatcttctgg gcgggcgtga tctggcagat
    10501 caacctgctg ctggcctcca ttttcaggcg gcgcaatact tttttccgcc tgccccaccc
    10561 tgccgtacgg gctacccccg caaaaatcct gaccgcaatg cttatattcg caggcgccct
    10621 tttggtattt gcatttattg catccgccgg attcaaaaaa atcccacata ttacagatga
    10681 agccgcctac ctttttcagg cccggatata cgccgagggg catctgtggg cgcctccgcc
    10741 gccggttacc ggttttttcg agcactatca tctgatgatt gaaggccagc gatggttttc
    10801 gaaatatccc ccgctgttct ctttggttct tgccttgggg gttttgagcg gctggccgtg
    10861 gctgatcaat ccacttctgg gcgccatgct aaccgttacg ctctacctga ttgcacgaca
    10921 gctgttctca tttcgaatcg ctttaattgc gagtatctta ttaattgtgt ctccactcca
    10981 catgttcatg tcggccacca tgatggcaca tacgctgaca gcactgtgcc tgtggcaaat
    11041 gatctatcaa tcccttgcgg gcatacagac cggcaatccc atgcgctttg cggcagcggg
    11101 tgcgctttgg gctgcagcct tcatgactcg gcccttcact gcagccgctg ttctgcttcc
    11161 cgcaagcatc ggagcggctg taagctggcg cagatgcttc acaagaaagg ccgcagggaa
    11221 aatgcttgca gcgtccgcgg cgggggccta cccgatactt gcgctgtttg cctgctggag
    11281 tgtgctgtac tgcagcgata gcgggcagcc catgatcccc tatatttcct atcacgccgc
    11341 cgacactctc ggcttcggtg ccgacaaagg agcaggctgg ctgatgacct gggggagctg
    11401 gggccacact cccgccaagg ccctgcgcag catcagtgtt tttctggaaa ataccatgca
    11461 gtattttcac ggatggcccc tcggaatgtc tcttgttttc gtcccgctgg ctttctggga
    11521 caaagcaaac agcaagcgcc cgatgttatt tctgctggga gtgtttctgt ccctcgtcgc
    11581 aggtcatgct ttctactggg ccacagaaca tctcggttac ggggcccggt actggtatgc
    11641 gggaatgccc ggcatcacgc tgctgtcggc tgtgggactg aactcactga tccgccaatc
    11701 agaaatttac ggtaaagata tccgttgcac cccgtcgatc ttcttcccta ttctgcttac
    11761 tggggttttc atattgtgga atgcattcgt gtatttgcct ccgaaattct ttgaggcgcg
    11821 cacctacggc ggcatctcat cgcgactggg cgatcaagtc gcggccgccg gtctgaccaa
    11881 cgcaattgtc tttgtgaaga cagaaaactc gctctacaac gacgggttcc acttgaacga
    11941 tccgcttctc cgcaaaggcc catactttgt gaacgatctg ggagcccgta atgatgaaat
    12001 gatgcaacta tatactgatt atcatttctt ccggtggaat aaaacaagcc ttcttccgat
    12061 tcgcactgat cttgccgcgc cgtgatcctc tattcttttt tgtacacggc cacagctctc
    12121 catgctcgcc tttgaagcgc cgggatcagt ctgaacagaa cggaatccag cgggttcagc
    12181 aaacaaagac taatccggaa ggcttgcacg cttctgcacc gcaaaagaaa ataaaaggcc
    12241 gccagcgaca gaaaataaaa cgccttgata tgcacaatcc tcatggttga agacaacgcc
    12301 cccatctcac cgtattccaa atagcgggtg aaccctcgct gtgcaccgtc cgatcccaag
    12361 cgacggtaca gtttgaccgc aggatggtcc gcaagcgcct ccatgaacac cgcgcgacct
    12421 ccgcgctgca gaacgcgcgc acattcgacc atcattttcc tccagtcccc gtacatcaga
    12481 acatccctgc tgaatatgaa attgaaggat gcatcgtcaa acggcaggca ttccgcatcg
    12541 gctgttacga agcggatggc cacctcgggg gcatgacggc gaaccagttc ccgcgcaagt
    12601 tcagttcttt ctccggcaat atcgacgccg gtgattgttg caccgcgacg ggccaaatga
    12661 acacattcgc atccgagacc ggaggccagc accaggcccc gcatcccctg cagctccccc
    12721 agaccgctca acgcagcccg cacatccggc ttcaggcaat tcctatatgc tttctcccac
    12781 cgggcatcaa tatctgaggg gcgccccatc tgctgattcc atggatctaa attccggttc
    12841 cagtaggatt caacacttgt acacgacttt ctagtatttg catgcatgat tagccgggtt
    12901 ttgatcataa tctgagaaag cttaatcttt attctcaagt tatagtatga tcaagcgtta
    12961 actgttacgg aagtcattaa tcggcttccc gttttaatta gaggttgaaa aggcaattcc
    13021 tgcggcccgc aacggagtgc gaaacatcca cctcaggggg agaagtctgg atgtatcgtt
    13081 gtcatccatc tttggggaat tgtccttagt aagtccgttc ttcttcgctg gtgaaggtgt
    13141 aaggaatcac aaggccatag cgggaagccc agctctgcat ttcctccagc acatcctggt
    13201 tcccgtcgcg aatgtagcgg cccatcagat catagatgcg atgccatacc cactgggacc
    13261 gtaatgcaga gcccccatcc cctgccccat cccaccgaag cggaaagatc atgtcataac
    13321 cgtgcccgag ggcggtcccc gtgatctgca gcaacacatc ttctgtatcc ggatgacacc
    13381 ggccatacag catgagcggc cgatccagat aaaggtgcgt cagcgaggaa ggatagatat
    13441 ccccttcatc cagaggggcg cactggtaat ccagcccggt aaggaccggt cgcgatattt
    13501 cgcgaagcag ttccgtcatg gtttcaggaa tcttctgcgg ttccatgaca acatgcgcat
    13561 ccccgcggtt cttgtaactc agaaaatcaa gcagcatgcg gttcactctc tgcccgccgc
    13621 ccacggtaaa aacggacaca cgcccctgat tggactgcgt gaaacggtct atgatttcag
    13681 atgtgtccag catcccgctg gtgggccgtc catcggtaat aagcacagcc acaacaggat
    13741 tggtcgcatc cacgctttgc gccagcacct gccgcagcgg agcgtacaca tccgtgtttc
    13801 cgcgcgactg catatcattg ataaaccagc gcgcgcgggc ctgcgacagg ggggtcaccg
    13861 ccactggcgc gggatagcat gccgccgtgt cctctcgaaa cgccatgatg ttgaattcat
    13921 cgcgctccgc gagccggttg acgcactgca tcagcccctc tttgcactgc tccacttttt
    13981 ctgccgtcat gctttcagaa cagtcttgaa ggaaaaccag ctgtttggag agaaccggaa
    14041 gggcgtcggc ggactgctgc ccgatcccga tctggaaata gagccactcc tcgtcgcgcg
    14101 gcctgaatac ggagatattg agagccagaa gatcttcaac cggttccatt tcggatattt
    14161 cccccggagc ctcttccagc aggctgccgc ttaaaccggc cgggacagaa tgcatctcat
    14221 cccctgttcc gattcctgcg gccggaaaaa taaccggcag cgccacgtct gccgcatatc
    14281 cgccgcccgg cggcagtgaa gccgcagccg ggatttcttc cggagtctga agccgcatgt
    14341 ccgccgcatc gtatgatcgc ggtacatcgt cggcaaagcg ccgcggaagc gcactgacct
    14401 catcggcgta aattcgctcc tctatggaca ccacctgctg ccgcgcatcc cattcggagg
    14461 cgggagccgc cgattgaaca ggcgctttcg gctcatgcgt ccaggccgac ggcgctgttt
    14521 cagccggccg cagaggcgaa agcgcggttt tctccatgat gtcttcagcc cagcggccgg
    14581 gatcctcggg acgaacgggt ctttccatct gcagctgttc aggccgataa tccggaagga
    14641 ccttgtccag cacgaccgca tgggccctct tcagcagcgc cagatccgac gcgcgcccca
    14701 gaacgaaact cggacagtgc ctgatcagga gcgcgtgcag cagcgaggat gcgcagactg
    14761 caagcagggc ggcatacaga tttttcgggt gccgtccccc ttcccgccgc agtatcctcg
    14821 gatacgctgc cggcattccg tcagccctgc agcgtaatca gaaactcggc attgctgcgc
    14881 gttttcttca gacggccaat cagcagttcc atggcctcta cagccggcac gccattgagc
    14941 gccttgcgaa gaatccaaat cttggccagt tcatcttcgt gcagcaggag ttcctccttg
    15001 cgtgtgccgg atttctccac gttgatggac gggaagatgc gcttgtcaac gagattgcgg
    15061 tccagcccaa gctccatatt gcccgtgccc ttgaattcct caaaaatcac ttcgtccatt
    15121 cggcttccgg tatccaccag agccgtggca atgatggtca ggcttccgcc gttttcgatg
    15181 tttcgcgccg cgccgaagaa gcgcttgggc ttgtgcaggg cgtttgcgtc cacaccgccg
    15241 gacaggattt tcccgctgtg gggctgaagg gtgttgtacg ccctcgccag ccgcgtgatg
    15301 ctgtcgagca ggatcacgac atctttcccg cattccacca tgcgcttggc catttcaatc
    15361 acgatctccg cgacctgcac atgcctctcg ggcggttcat caaaggtcga gctgagcact
    15421 tcggccttgg tgttgcggat catgtccgtc acttcctccg ggcgctcgtc aatcagcaga
    15481 atgacaagct tcacttcagg attgttcttt gaaatgctgt tggccatttt ctgcatcagg
    15541 acggtcttgc cggtgcgcgg gggagccaca atcaggccgc gctgcccctt gcctacgggg
    15601 gtcatcaaat ccatgacccg catggatatt tcagcgggat ccacctccag taccagacgc
    15661 tcgtccggga acagcggggt caggttttca aagggcacca cacggcgggt tttttcagga
    15721 tcatcgttgt tgattttctc aaccttcaac atcgcgaaaa agcgctcttt gtccttgggc
    15781 ggacggattt cgcccagcac caggtcgccc gtccgcaggc cgaagcggcg gatctgcgac
    15841 ggagagacat aaatgtcttc cggacagggc agataattat agatcggaga gcggagaaag
    15901 ccgaagttgt cgggcagaat ttccagaata cccttgccta tgacattgcc gccgcagcgg
    15961 gcgtgggcct tcaggatttc gaaggtcagc tcgtgtttgc gaagagcgcc aagactttcg
    16021 agcttcagtt catcggccag tgcaagcagt tcgggaaccg tctgacgctg catttcatag
    16081 agataaagcg tcatgcccct gcggtcggtc ggggatttct gcacagccgc cggctctcgc
    16141 tctgcgctgc cgccgggcgc ggcctcggcg gtcggcacgg cctcctgtga aacaggggtc
    16201 tcaacgggtg tttcagcggg ggccggctcg aatgtttcgg cctttctgcg gccggggccc
    16261 ctcctcgcat gctttttctt cgaacccttt tcctgatcgc ccgatgtcct ggtgttttct
    16321 ctgtctgtca taacgaccgt tcccttttgc tgataatttc cctgtaaaca actgaaatgc
    16381 tttctttcag ctctttcagg cttccgttgt tccaaattac ataatctgcg cgccggcatt
    16441 tttcatccag cggcatttgt gcccctattc tctcagtcat ctgctgctcg ctcatgcccc
    16501 gttcacggag ccgggcgcgc acaagggctt cggatgccgc cacacatatg accgcatccc
    16561 acccggcggt ctcgccgacc tcgtacagca gcggaataat gaccgccgca tcgcgacctt
    16621 cctcgaaacg cgcggccgtc cactgccgcc agcactcacg caccgccgga tgaaccaacg
    16681 tgttcaggag ttcaaggcgg gcccggtccc gaaacacaat ttccgccagt ctgccgcggt
    16741 caatttcccc ggatgtcccg acgattgcgg gaccgaatgc ctgcacaacc tcgtcataga
    16801 cgggcattcc ccggcgcatg agccgatgcg cctcaatatc ggcgtccatc acggccacgt
    16861 tcaagctctc cagcgccgcg cctgcagcgg attttccgca tccgattcct ccggtgagcc
    16921 caaggagcac cgggcttctg gccgcaatcc tggtcataaa tgcccgctca tgaaattctg
    16981 ctcccgcagg actcccacat gtttcagcac attcatggcg cccgtatgcc ggtcatcagc
    17041 cttcagcgcc tccaccgcat aatattcggc attgtcgaga tttccatgct gcagttcctc
    17101 ttccgcgcgg gccgccgagc gggccgccat ctcttcatcc gcttccgaaa taatctcaaa
    17161 gaccctgtat ttccggttcc cccagcgata tctgaaccag cgcccctctt cgggacggaa
    17221 ttcaaacata cgcgcggccg cctccgcagg cggattcagc gcattcacaa agtagcgcat
    17281 ctgcagttcg ggcgatctcg acgaaaactc gcccagcgca tgcacatacc actcagcccc
    17341 gtactgaccg gcccaggcgc ggaattccga ctcgtcgccc ttgaagagag cttccccata
    17401 gcgctcaaca cgccggcgga tttccactcc ctcaaatttc gggtgcagca cgatgggcga
    17461 cccgccatac gccagaatcg aggcgctcac tccaaaattc gccgccaccg gcttctgttc
    17521 gccgtttttc gcgaaccact ctgtcagttc atcaagttcg cggtaatata cattggtccg
    17581 cccccactgc tccggctgag ccagagtgtt ccgggcttca accaccccga cacatcccgc
    17641 gagcaggaca aacgcccatc gcagtgccgg ggttcgctcc ttcagctttc cgtacagcac
    17701 cccaggcagc gccgacgcaa aaacggccag ataaacatga aatctgaaaa agaaccagaa
    17761 tgtgattaac gaagcgccat agaagaaaac gaactgaaac aactcagatt gcgcacgtct
    17821 caattgataa aaacagcata cacaaaatgc gcccaaagtc aatcccaaca agccgggaaa
    17881 gagcagccgg gcctgggccc aggttgtgga atgcagtgca ggcacccaca tgatgcgctg
    17941 gctgaagctt aacagcgacg gatcagccgg tttgtggttg agaaagcgaa tcttcgccca
    18001 gagcagttcc ccaaagtgcg catagctccc ggagacggct gtatgaatca caatccccag
    18061 aagcaagggc aatgcgagca ggagcagata atgccggcgc aacagatgca cgcccggtct
    18121 ctgttcaaga atgcaggcag ccgtcacgcc gcaggccagg agcatcggcg gagaaatcat
    18181 ccagccatga gcccgcatat acggattcag caggctgaag aacatcaatg ccgtccattc
    18241 gaaaagccaa agttcacgaa aggccggacg ggacacaaac ccgcccttca gcagtctcac
    18301 cgcgccggcc acagcccaca ggaacacata gaactggaca aggtcccagt tgaccgccgc
    18361 cagactcaaa cacagcgccg aggaaaaggc ccacaatctt gcgcggggga agccgcagac
    18421 agcccgcgcc gaaaatgcca gatgggcaat cagaaaaggc agcgcaaaat tctccctgga
    18481 tatctcctgg cccgtggagc ggatcacact ggaaattgcc accgcataga aaaatcccga
    18541 aatccatccc gcagccggag aagaggccgc ctgccggatc cagaggacca tcagcggaat
    18601 tccgagacag aaccagagca cctccatcca ccgaatgcgt tcagccaatg gcattctcga
    18661 gggccagcag cgggccgtga gggcgtaaaa atattctgaa ccaatcgaat aggtccgccg
    18721 gacattcacc ccggaaggat attcaacaga gaagtccatc tcaggcagac gaccggccat
    18781 ggccgtctgg atcatgcgaa aatgcagcgc gctttccagc gtgaaggcgg gagattcgcc
    18841 cagggccctg tactgagcct gaagcacctc gcgacgaatc ccgaccccaa gggcataaag
    18901 aacaacaagt cccagaacaa agagaatatt tctgtacttc aatttcattg cattgaaagc
    18961 atgacggcga ttctgcaagc cgctcatatc ggcgcgcaca gcgtgcgcgc ccgacggatc
    19021 tacaatccga gttctccctg caccatgcgg gagaggctgt gcaactcata cttgctgaaa
    19081 aatgacacaa ggcgcgaacg atccccctcc cggacggcta aatcctccca gttcagcgaa
    19141 acattcaaat cggtccgcag cttcatcagg cctgtgttcc gccgaactgt cggctcgtgc
    19201 tgagccagca caccgcggaa gcgggccggc tggatttcgg cggcatgttt tagaatttca
    19261 tccagtgtgc cgtattcctt gagccatttc gccgccgttt tggggcctac acccggcagt
    19321 ccgttgatat tgtccaccgc atctccaatc agcgcctgat aatcaaccat ctgatcgggc
    19381 ctgactccgg ttttcgtcac aacctcctcc ggccccatgc aaaccatctc ctttgcgggc
    19441 gagaccacac atatgcggtc actcacgatc tgatacatat ccttgtcgct ggtggccata
    19501 tagaccgagg ccccctcttc ggcggcgcgg actgcgaggg ttgccatgac gtcatccgcc
    19561 tcctgccggt caagccggaa tgagggcact ccggctgctt ccaggtattc gttgatatag
    19621 gggatttgct ctttaagagg atccggcatg ggcgcacgct gggctttata ggtttcaagc
    19681 gccgccatgc gctcctcagg acggccgccg tcaaaagcag cggcccagtg agtgggtttc
    19741 caggccgact tcagctgcag catcatgcga atgaagccca taagagcatt ggtgtggcgg
    19801 ccatccttcg tcgtcaaatc cgggatggcg tagaaacccc gataggccat ggcgagacca
    19861 tcgagaagaa gaaggctttt cgcgtttgtc atgatttgct gtactacgac ccctgactgc
    19921 acgaggaagg catcatgcag acatcccgcg ctgtgaaatc aaaggtttcc ggccagccgg
    19981 attctacttc atcgcggttt catcaaccgc ggtggagcca ccgggcatag ccatcatatc
    20041 ggccttatga atcggtttcc cggcgggatc aacaagccgg gccgtcacaa atatgagcag
    20101 attgcgcttc tggctcattt cgcccttgct gcggaacagc gcgcccagca gcgggatatc
    20161 acccagcacc gggatcttgt cttccatggt aaccatttcc tcacggatca ggccgcccat
    20221 aacgaccgtg gcgccgtccc agaccacaat actggttgtc acattgcggc tgctgaagac
    20281 cggctgcggg atattatagc caatgggatt tccatcctcg tcgcgatcaa tgactgatcc
    20341 gtactgaatc cagtccacca gttcggatac ttccgggacc agcgtcagat cgatagtgta
    20401 gccatccggt cccacagtcg gggtcacatt cagaatcacg ccggtttcac gggtttcaaa
    20461 cgaacccggg gtcacggtga caaggcgttc ctgaatggtt ccaccaccgg ccacttcgcg
    20521 gtcaatcgtg atcacttcac tttcaaacac ggtgggatat ataatctcct gcaccacctg
    20581 aatctgggca ttgacgccgc tgcgggtcgt aatgcgcggt gctgacagca ggtcgcttcc
    20641 gcccttctgc tgaagagcgt ggataaccat ctgcagttcc ggattggtaa gcacgctgga
    20701 gaaggacagg atatttccca tggcggtgct ggttccccgc gtgatggccg acgccggagc
    20761 catcgcgccg gtattgccgt caaacccgaa gaaacgcagc ccctgagtca ggccgctcgg
    20821 attcgcatca gcctggatgc gttcctgccc tgcgataggc gcattgcctt ttttgcgcgc
    20881 gatttcatag ttgtcggtaa ggatccactc aaggcccagt tcctcaagat cgttctggcc
    20941 gatctccaca aaacgggctt cgatctcaac ctggctcgga atgacattca gctgtgcgag
    21001 aatgcgctcg aagatttcaa ggttttcagc cgtgttggcc acaatcatct ggctgatgct
    21061 gggattataa gtgattgacg ttccggccgg gaacggaact cccgcatcag tgaagaattt
    21121 tttcacatcg ccacgggtca tggtcacgct cgacccggtc atttcgacaa actcaccggt
    21181 cttttcggtc tgttcagaac cccgctcaac gatcacgtca atgatggacg gctgcaccgg
    21241 atacatgcgg gtaataatca cgcccgtggc cacaccggcc ggggtgataa tgaccacgtt
    21301 cccatcaatg cgatagcgca ggttggaaac ctcggtgatg attttcagag cgtcaatcag
    21361 agagatgcgg cgcagattga gcgtgatggt cgggatggaa gaaccgccac cgccgccgcc
    21421 aaagtctgaa ggctgttcca tcataccggt gtcaaaaccg gggaaatcag caaacggatc
    21481 tgccggcgct gcaggagccg gcgccggggc gggcgccgcc tcgccgggct ggttcaaatt
    21541 aaggatgatg ttgaccccaa cgccctcggg atcgcccgcc agactggcgt ccaccaggaa
    21601 gttgaccaca tcccgaatgt tggcctggcg gaattcaatg gatggaatca tgatggagga
    21661 catcttttcg cggagttttt ccgtcgtggt tttggtgtcg atggattcct gcttcacaac
    21721 ggtttccgga agttcgacct ctcctgtggg cggattccat ttttcacgca ccttgcgcat
    21781 ggactcagcg actgtggctt tgcgctccaa tgtgcttaca cgataccagt gctcttcgat
    21841 gcggcggaga taccgcatgg cgtcggtgtt gaactcgtca acgaccagaa cctggtcaaa
    21901 gagtgcttct gcggatttat agtcgcccat gtcaaaatac tgccggccct ctgcaaccag
    21961 ctcttctgct gttttctttt tctcaatgac aaccgggcga tcctcaagag gaacgggacg
    22021 ggcggcaatt gcagcctgac gctcgatatc ccgtgcaaga cgggtcttga gtcgtgcgga
    22081 cggcccatga gaaggatcaa gtttcagcgc ccgctcgaca ctcttcatgg cggcttccag
    22141 ccgtgcaggg tccctggcca tgttgcgggc atcccgatag ctggcctcaa cgagacccca
    22201 gatggcgtgg tccctgttct gctgtgtggc cggacgcgga gggagcttgc tcagggcatc
    22261 ctcaaaatat ttcacggcgg ccgcgaaatc gctctggtcc agagccttga atccggaatc
    22321 gatcgcctgc agagcgctgg tttcatcggc ctggcgccga acttcttcct gctgtgcacg
    22381 aagctttgcc gttttcttgt caacgaccgg ctcatcggac ggagcctttt cttcgggcat
    22441 ggcttctgcg tcaacctcat cggtcatgac ttcttcctcg accggcatat ccatggtctc
    22501 gtctggaagg ggttcctctc ccatggagga aaacaaatcc tcgacagccg aggcttcctc
    22561 ttcctcaaaa acaggctcca gttccatttc gacagaggga accacgtctt cctcgacggg
    22621 ggcttcaaac atcaggtcgt cctgcacagg cgctgcttct tcagcgggca gttcttccgg
    22681 gatatccatc acgggttctt cagaaagaaa agcgtccagg tccacatcgt ctgtcacaac
    22741 catttcgggg gtgtccatgg gctcatccat ggccggcagg tcctctgact cgaacgaagg
    22801 catctcttct gcagccgcct cttcaacagc aggagattcc tctcccatag gaaattcatc
    22861 ttctgccgtc acctcttcat cggcaggaag ttcggccgcg gcatcttccg ccacaacggc
    22921 aggagcgacg gagggatctc ccgtcagatc ttccagaagc atatctatat tgaggtcgtc
    22981 ctgacctgac acctgcactg cgggattcag ccacatacaa agcatgatgg ctacaatggc
    23041 tctggccaat ttcatagata ttctccttct ggatatgtct gattcagctt tataaattcc
    23101 aacaaaactt ttcctcattc ctgccggtct ctttcagccg gcggaaccgg tcgccccgaa
    23161 acctcacggt tcagccggcg gagggaactg cgctgccgca ctttccactg cgcccggttc
    23221 tgcctgcatc gccgagggct ccgatattcc gcgcaaaatc atgcgttcct cttccgtcag
    23281 cggaggaacc tgtatctgct tctgcgtttg ttcgtcctct attaagacgt gtgaagggtt
    23341 gatgtcaatg actttatgcg ggcgtccctt gacatcaatg gcgtcattca cccgcacctg
    23401 atactgacgg ccgtccagca gagacaccag cgcggctatg aattcctttg cagtgatggg
    23461 ctcatttttc ttgagctgaa gcgacttgcc gtctttttcc agcgttagga ttgaagaagc
    23521 ctcatcatag gccgcgacct tgtacccctc caccacggcg cccaacttgg caaagtggct
    23581 cttatccgcg ctgcgcaggt tcaacaggaa actcttgcct gttgccagct cgctcacgcc
    23641 ctggaaccgg aggaaaaacg gcttcacgac catgcgaacc atgcggagct tggccgccgg
    23701 agacgggtgg tcctccggat cggtgggatc cgtcttgttc tggaactcct ccacatttga
    23761 aaacccgtcg ccgtccaagt ccagtgcgga atcgccggga tcatagggat tgagtccgaa
    23821 cttcttctcg aactggtcgg gcatcccgtc cccgtcatag tccatgcccg caatgtcttc
    23881 acggctgggc tgcgcggcct ggcagaatgg acatttggcg gcgttgaagg caatcggttt
    23941 ttcgcatgag ggattgacgc agtataccct gagctggctc accagcatca ggttggactg
    24001 aatcgtcatc tggaacggac tccgcatcgc ggcgtccttc tccacccaga ccgcgggatc
    24061 catttccgtc gcggtaatgc cctcctgctg cgccacgccg gagccgcgaa ccgacgaagc
    24121 cgcatcgccg ccgatcaaat tcagcgccag cagtatgcaa agagctgcaa gcacaatgag
    24181 cgccacatcg aggatcagct tctcatagga agtggccaac cagcttgttt tttctctagg
    24241 ggccgcactc actccgccgt ctcctgggtt gaggtctggg ttttgaacct gtagatatca
    24301 aattcgattt ttgccgtgac cggttcacgg cctgcaacca cccgttcttc tgcgccgggc
    24361 acaatggcgg gcgcagccgt tccggcgagc gtcggcacgc ggccctgctg gaacatgcct
    24421 gttctctgag actggcccga catggcaccc cgcgcggcaa gatcggcggc cgttttggag
    24481 gcctgtttga tcgcttcgac agcccgtttg atatcaggct gatcactgct cagctgaatg
    24541 ctggtgacca cgattttcat gtcattgcgg gccatttcat tcagaaatct ccaaatggcc
    24601 tcctcgcgac cccggacttc cagaacgaag tgctcggaag aataaagctc actctgcttt
    24661 ttcaaggcaa ccggaccgga agcctccgca gcatcttctg ttcgtgccgc ccgcctggcc
    24721 cggcgggccg cgcggggatc agacgcatcg atttccgcgg ccgcagatgc cgtgccttcg
    24781 aacagctccc ggttgaagga tacaatctcc cccagcccga cagagaacac cagccccagc
    24841 actttctctg cctcctgaag ctgaacaatg agccggctga tctcattcgt gttctgcgga
    24901 agttctccct tcaggtaacg gtcaaacccc atggagaaac cggcgggcag cgtcaccttc
    24961 gcttcctcgg cggccgaacg caggcggcgc acagagcgct ccagcagcgg cgggaattcc
    25021 gcgcgctcca tgggcttcgg ttcgatttgt ccctgcagga ggtcgcccat cagcgcttca
    25081 aaccgctgct ccattagatt tgcgttttca gcaatgcgct cgacattttc tctggatgga
    25141 tagggatcac gccgctgcag ctgcgtcagg cgagctttct tcccgtccag atccgacttc
    25201 acggtgctgt agccctgcca gactttgatg aggaccgcgg ccagcacaat ggctaccact
    25261 gcaataacgg ccgccccaat gatcagtccc agtttatcct tgcgcaagtt cataacggca
    25321 ggccctcctg cagcacaatt ttgatttcga actcgcgggc gaaatcatct gcccctggag
    25381 cgggctgccg ctgaatctcg gtttcctccg aaacaaaatc gagcttgcga aggttgtccc
    25441 ggaactccag gatgggagtg gccgaccgga ccacatccag gtatccccgg cccttgaccg
    25501 cgagagccgt aacaacgccc ggcgctttgg gctgccctgc cggagcggcc gggccgctgt
    25561 cgacaaccgg cacgatggaa gcaatccaca tgccggatgg aagggcggag tgaatctggc
    25621 tcatcatgcg gatccagagg cttctctggt tcatcaaccc gtaaatctca tccattttcc
    25681 cggaaatatc gccaattctc tgctcttcgg ctgaaagctg ggattcgatg gccgcaaggt
    25741 cgtcaacccg ggccgagata tccgcgagct gcgatgccgc ataggatttg acccgcgcga
    25801 tatagccggt gacggccatc atggccagaa ccgccgcgac gccagcgccg atgaaataag
    25861 gccttttttt gcgaagctct ttttccttga ggactttctt gggcatcagg ttcagttcaa
    25921 tcgggcagga aaggactttg cggaggccca gaccaacaag ctcgcccatc aggtgcgcgg
    25981 atttcccgat ttcctcagcc gagatactgg cccccaccgc cacattcctg aagggattca
    26041 gataatccac ttcaaccttg agcttctcct taaggaaagt atccgtatag ggaatcacgg
    26101 aagtgccgcc ggtcaggagg atcagctccg gctttccccc accctgctga ctgcggtaga
    26161 aattgatcga gcggttgatt tccgcatgca tgcgcgtcat gatggaacgc acgctcttcg
    26221 acaccttgtc cgccacttcg ctggccggac cctcataagc cccgccgaag gccacaaagg
    26281 cgtgggctcg cttcagatct tcggcgtccg cgtaattcat gttgaactcg cgcatgatgt
    26341 gctgagtgat cgtgttgcca gccaccggaa gactgcgtgt aaagatgcgc ccctcttcga
    26401 tgaatatcag gtccgtcgag cgggcaccga tgtccaccac cagagtgcag ccggccagat
    26461 catcataatt gtaccggacc gtgttgtaga gcgccatcgg cgccacatcc accagatccg
    26521 ggtcaagccc ggcttcgccg accgaatccg tcatcttttc gatcatctcc gccttgatgg
    26581 cggccagcag cacatccact tcgccggtcg cgccgccgat gagctggtag tcccaaacca
    26641 cctcatcaat gggaaacggc acattttgct gtgcctcgta gagaatgatc tgatagatcc
    26701 tctccgaatc cacaggagga agcttcacat aacgcgagaa gaccgactgg cccgcaacag
    26761 acaccagcgc aggcccgggc tttatgccgg tttcgcgcat catgcgccgg acggcctgca
    26821 cgatgaatgc gccccgatcg acatcactct gggaatccac tcccagtgat tcacagctga
    26881 agttgatcag ttcaaggccc ccggacttta gcgcgacaaa ttcagccatc ttgaggctgc
    26941 tggcgccgat gtcgagtgca agtatacggt ctttgttgag catattgttg ttacccgaag
    27001 gatttacttt gccggtgccg gattgatggc ctcgtattca tcgtactgca gaaaagcaaa
    27061 gggcgtctgc agacggttga gcaccagccc gtcccggatc ccgggcagca ggttcgggtc
    27121 ttcccaccag cggcctttcc cctggtcgga gctttccacg gcgaccgaaa cccccttgtg
    27181 cttgatttcc acacctgcgc ggtacaccgt tccataccgc cccagagtag agggatgcag
    27241 atacatcact tccttgtgac ggcttccacg cggaacattc acgcaggaca cggtgccccg
    27301 cagcagctga tatctgggat ccttgccctg cagcacaaca taaaaagtga aatccagctg
    27361 ttctgtccat tcaggagcgg tatcatattc gaccatcacg gcaaaccagt ctttggccgc
    27421 gccggaggtg ttgaacccgg aaaccttgaa gtccggagtc ttgaccttgc tcccgctgac
    27481 ttttctgatc cgggcgacat cctgagcgcc ggcctgcgca gaggcggttt gagaaataaa
    27541 ccccagcagg gcggccagac atgcggcaag aataagttgt gtcttcttca tatcaatctg
    27601 cctcgttcgt ccggcggact gctgtgccgg attctgccga ttacaaatca aacagatgca
    27661 gtatagaaat cccaactgcc agcgtcaaca ctacttgcag aaaagttgaa tttctttttc
    27721 cgcaaacaga tgttcattcc gccgcagcat ccctcaggga gccccgaaac gcgcaaatgc
    27781 cccgcttgga gttcgctatg aactcgcaca tctcaaggac ggcggaagat gaatatgcgg
    27841 cgcgtatctt ctccactgcc tgcgaaacat tgagtccgga agcataaagc gtcttgaacg
    27901 cgcttcggat ttcggcgcgg gcggtcccgt caaacccgtt gcgacgaagt cccaccacgt
    27961 tcaaaccggc gatggagttg aacgcgccgc tggcgtgtgt gcagaaaggc gggatgtcct
    28021 tgcttaccgc cgaaagcccc cccaccatgg ccagccgccc gatccggcag aactggtgaa
    28081 ccgctgcgct gccgccgata aacacgccgt ccccaacctc cacatgcccg gccagcagga
    28141 cgttgttggc cagaatcacc ctgctcccga ggcggacatt gtgcgccaga tgggaatagg
    28201 ccatcagata gcagttgtcg cccatcaccg tggctgaccc ctccatggtc ccccggtgga
    28261 ctgttactcc ttcgcgaatg gtgcagctgc tgcctatgcg aacaaaactc ttcacgccct
    28321 tgaatcccag atcctgcggc agatcgccca gcacagcgtg cgcatgaatc cggcagtttt
    28381 ctccgatttc cgtgaattca tgaatcacgg catgcgggcc gatctccgtg cccggcccga
    28441 ttttcgaccc ggcccggaca taggcaaacg gcccgacact gactccggcg gccagttccg
    28501 cgcccggttc aacaacagcc gtcatatgaa tatcagaaga tgccatcagt ccctctcaaa
    28561 ctcgaccagt ccggtctcgc gcaggctgat ataatcctgc tccgtctcgg tttttctgtg
    28621 gccgacaata acatggtcca gcacacggat gccaatgatt tttccggcct ccacaagctg
    28681 gcgggtaagc gccacgtcct ccttcgacgg agaaggatct cccgacggat ggttgtggag
    28741 caggaccacc gcctgactgc ctgactggat ggcctgcttg aaaacgtccc gcgcatggac
    28801 cagactggca tccagaagcc cgcgcgaaac ttccaccggg gggcccttca attcattgcg
    28861 gatgttcagc atcagcaccc agaatacttc gcggttgagt gtgcgggcct gcagccggac
    28921 ctggtccgcg gcctgagccg gcgtgaggat tttctcgcgg gggcgggcgg tttccctgcc
    28981 catgcggacc gcaagttcca gggcgcactt cagaacctgc gcgcccactt tccccactcc
    29041 cttgaccgcc tgcaaatcct gcacggactg tttggccata ttgctcaggc ttccgaacct
    29101 ctgcatgagt tcgtcagcga ggaccgtgac atttttcccc gggatgccct tgcgaaggag
    29161 tatggcaagc agcgtggaat cggacacatt ttcggcccca tagcagtcga acagttcacg
    29221 cggctgttcc cgcaccggca gctgtttcac gggtatgctg taaaccaggt tatccttttt
    29281 catcctcgct ccagtcatca ggggcggccg catggggcgg ttcgccgcgg atatagggtc
    29341 tatacaggtg aaggatgcgt tcaggcaccc acgctctcac atggaagccg tcctcctcgt
    29401 acttctcatc ggccattctg gcatttgcgc gcaggcccgc aatcagacgc gtctgatcca
    29461 gcgggataca gagctcgacc tcggtgtggc ggtggcgcag cgtgtccgca agctgctcct
    29521 tcagatgatc caaaccctcc cctgtgcgcg cggacaccat cactgaaccg ggaagcagtt
    29581 ttgacaagcg ccgagcctgc gcgagtccgc tttcgcagtc tatcttgttc aggacagcca
    29641 ccaccggctt gtcgtccgtg cccatatccc ggaggacatc gttaaccacg ccgatctgcc
    29701 gttcggccag cggatgcgag gcatcaatca catggatcag gagatcggca tccaccacct
    29761 cctcaagcgt ggccttgaag gcctccacca gatgatgcgg aagcttgctg atgaatccca
    29821 ccgtatccgt cagaagggcc atctgctgat tgggaagttc cactctccgg gttgtcgggt
    29881 ccagcgtcac aaacagctgg tcattggtca ccacactcga cccggtcagg gcattcagca
    29941 gggttgattt tcctgcattg gtgtagccca cgatggaaac agacgcccag ccgtgccggc
    30001 gtcggccgcg ccgcagttcc gagcggtgcc ggcgcacccc ctccagctga tccttcagcc
    30061 gctcgatctg ctgacggatc cggcggcggt ccacctccat ctgctgctcc ccggggccgc
    30121 cgcgcacccc gatgcctccg acctggcgct ccagatgcgt ccacatgcgg cgcagccggg
    30181 gaagctgata ctccagccgc gcgagatcca cctgcaggcg gccctcgtgc gtatcggcgt
    30241 gcatggagaa aatatccaga atgatctgcg tgcgatccag cacgggcagg ccgatcagct
    30301 cttcaaggtt gcgtgcctgc gcgggagaga gatcctcatc gaagaccacg caccgggcgc
    30361 catggagctc agcgagactc cggatctctt ccgctttgcc tcggccgatg tagtatccgg
    30421 gatccggttt gggccggcgg cagatcagcg tgtgaaccac ctctgcccca tccgtttcca
    30481 caagccggga aagttcatcc agagagtcgc ggatttccca gtcccgggcc tggccgatcg
    30541 ccacccccgc cagcagggct ttctctttat ttatttccgt ctgcatcatc ggtcacacat
    30601 tcaggcgcac cgggtcctgt ttcgcccatg cggcgcaaac ctgttcggcc gtttccccga
    30661 cagtcattcc gggtttcact tcgatccatt ccacttcagc ctggtgacgg aaccacgtca
    30721 tctgccgctt ggccagctgg cgggtccgaa gcaccgttcg ttcttttgct tccgcaagcg
    30781 acatcttccc cgagagtacg gccgccgctt cggcatagcc aatggcctgc gcggcggttt
    30841 ctgaaaaccg cggatgctgt tccctcagcc gacgcacctc ttccatcagt ccattctggt
    30901 acatcaaatc cacgcgccgt gcgattctct tttcgagcgc gcccctttcc atatacaagc
    30961 ccgtaattct caccggcggg agctccgcct tcggaggcgg cgcaccgctg tgcgcgattt
    31021 cgatggcgcg gatcaggcgg cgcgggttct cacggtccct gactgaagca taccagtcag
    31081 gatgctcgtt cagaagtttc tgccgcagag cctcgagacc cctttctgcc gccagcgact
    31141 ccagttccgc acgaagggcc ggatcgggcg gcgcgccggc atccagcccc tgcagaaggg
    31201 ccttgatata gagtccggtc ccgccggcca cgataacggg tgcgtccggg aggctgacgc
    31261 gcacatgttc cagccaggcg ccggcgctga acgtgtccga gggatctgca agatcaatac
    31321 ccagatacga taccgccg
//
LOCUS       DAOQWR010000012        29737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1832_length_29737_cov_5.250590, whole genome shotgun sequence.
ACCESSION   DAOQWR010000012 DAOQWR010000000
VERSION     DAOQWR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29737
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_29737_cov_5.250590"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKK36_01965"
     CDS             <1..1020
                     /locus_tag="PKK36_01965"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNR93353.1"
                     /translation="WRGTGLEDYFNGGWYYQNFLARPLNGIVFKAPFRAVQYRIHFAD
                     PVMFHSAFEMNFERGPEMASRGVYESVAYYYLKTPQRSPSEIAQDASRYFPDDPMARA
                     TYMTEVNNIERMDDYAGAAEYTEYFLEKNPEFPFAETLRMRRDAYREKIRGSSAAPEF
                     YKKYLSSSNEVARKQAGDLEWFYADPSRALLGLYCNAPSRAFLDGKFVLGAGRDDRCE
                     IARLSLPDGRHTFAVQAQWKQYPDWMQFCLRTHSGDVVSRPDSKYALDAAGGWMNPDY
                     DDNAWPRLSGTGVKGPPEAPYVWMEPHPHIGMHSGAAGLRPEWNNRVQSYVVYRSEFD
                     VPSGR"
     gene            1063..1938
                     /locus_tag="PKK36_01970"
     CDS             1063..1938
                     /locus_tag="PKK36_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR93354.1"
                     /translation="MKMWKMLLFAGCAWASVCAFSGCSRNVANIDEQEERHPLMQRAL
                     NHKRLQDIDKAIDLFEEILRENSDMARAHLELGLLYDSYREDYMRALYHYQRYLELRP
                     DTEKREMIENIMKMARLSFAATLPHSPSDTLDEIAALKRENAMLTTRINEMSRALNLA
                     EKKAAEAARREESALLARVPDPPARLADTRPPDVQADRPPMDVSPDRIPKPAPAAAPA
                     RPQSRPAPAAAVKPPPPAPPVAQTYRVLRGDTLSSIAAKMYNNPQMWTKIFDANRDKL
                     DRPEGIRVGQVLVIP"
     gene            1962..2708
                     /locus_tag="PKK36_01975"
     CDS             1962..2708
                     /locus_tag="PKK36_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93355.1"
                     /translation="MGATDFFDDDLVKPGRKEGSISMGPGAGSTKSVEPGAPSTTFPS
                     RPVSDFNLTRMARHKDEVETQVARAAEELERLRQRQEDLEREKHDLEDLRRMQDEYQQ
                     GRREMIEQLSQNLLKLEKDELRAEQTRELLASTRRSFKAMLENIKGLNDETWTDEQFR
                     EELGKARTVIEDARIEFNKARAKIDALISIEEKADRGRPVVFESPLREENVFSFGHWF
                     KVGMAVTLPLTILIVLLMAAFAALRYMRVL"
     gene            2726..3094
                     /locus_tag="PKK36_01980"
     CDS             2726..3094
                     /locus_tag="PKK36_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93356.1"
                     /translation="MGKGKSALDRRLEQLEREAASIQSNISSLDYALRRADRGAPARL
                     KLMPDRRSLSAHPAPERKTKDPVEKPVRRKIANYLSGGSFRAVKLTPQEMAVQRNKAI
                     FMIIVLIIVIYVMLKLFHKV"
     gene            complement(3124..3600)
                     /locus_tag="PKK36_01985"
     CDS             complement(3124..3600)
                     /locus_tag="PKK36_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR93357.1"
                     /translation="MSAEKEIIAFALSDTHGHLPDGALQMLHGADSIFHAGDIGSPAI
                     LDQLSKIAPVHAVRGNMDRHSWAGRLSSVEVLELGGHTVCLLHDLAAADVDFRAMKAA
                     LVLHGHTHRPSISSREGILFVNPGSISQPRSDAGPTLARIHIGRDIRAEIIAMVLP"
     gene            3656..4228
                     /locus_tag="PKK36_01990"
     CDS             3656..4228
                     /locus_tag="PKK36_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR93358.1"
                     /translation="MSSPVGHSVIGAAFGVLYFLKPAGRRGLWVQAAEKWPWILGAAL
                     FANLPDIDYIPGVMAGELNLYHHGVTHTLGWAALVSLLVWIPLRPKGARGRELSLIFL
                     LLFSHLALDWVTADGKEPYGIMILWPFSNNYFISEVPLLPHLRKADFTELLDPYNFRI
                     MAVEILRTLPVLLLALACKLFGGRRGAPPL"
     gene            complement(4257..4766)
                     /locus_tag="PKK36_01995"
     CDS             complement(4257..4766)
                     /locus_tag="PKK36_01995"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNR93359.1"
                     /translation="MSEKSCHITGFRWPSPPLRLVLVEPEIPPNTGNIVRLCAGTGSE
                     LHLIEPLGFQLTESAIRRAGLDYWEYARIFRHRSFQAFQKDINPERIFFFSTRGPRLY
                     TDMAYQAGDALVFGSETRGLPPEITDAHPEELVCLPMKDIRVRSQNLANAAAIVLYEA
                     LRQINLQDG"
     gene            4858..5085
                     /gene="rpmB"
                     /locus_tag="PKK36_02000"
     CDS             4858..5085
                     /gene="rpmB"
                     /locus_tag="PKK36_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNR93360.1"
                     /translation="MSRICEVCGKGPRTGNTIVRRGLAKSKGGIGLHTTGITRRRYLP
                     NLQKIRVVENGGVKTRRVCTSCIKAGKVSKV"
     gene            5169..6467
                     /locus_tag="PKK36_02005"
     CDS             5169..6467
                     /locus_tag="PKK36_02005"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNR93361.1"
                     /translation="MSDSKAQLARLEINGRKCEFPVLTGSEGEQSVDIRRLRKETGLT
                     TFDPGFVNTASCQSAITYLDGEKGILRYRGYPVEELIKICNFIDVAYLLIHGVLPNEQ
                     VRNDFARLLNRHSMIHESMRTFFDHFPENAHPMAILSAMVVSLSSFYPEMESDQEGVD
                     MAATRLLSKIRTIAAFSYKKSIGEPVVQPSYKLSYCENFLNMMFASPVANYKLDPVVV
                     RALDQLLILHADHEQNCSTSAVRLIGSSGANLYASVSAGICALWGPAHGGANQGVIQM
                     LERLQAEGGDAKKIIDRAKDPNANFRLMGFGHRVYKTYDPRARIAKQLCEEVLGKLGI
                     NEPLLDLAMKLEQAALSDEYFIERNLYPNVDFYTGIIFRAMGIPTDMFTVMFAIGRLP
                     GWIANWLEWMSDPAQKIGRPRQLYVGPKKQKVPPRKQGGF"
     gene            6473..7330
                     /locus_tag="PKK36_02010"
     CDS             6473..7330
                     /locus_tag="PKK36_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93362.1"
                     /translation="MRSAMTRTGRQHFLSWILFITAPLLAVSSEPWSLEADIAAGGGR
                     LSYEEDSAGLESEWSAPAFILEGRARLRAPRRLLFAASSRLTVTDKDQEEWSEDGLVR
                     QENQLHLAGVHALGEAGWAVVQAEALQAAILLGMGARYQHFVRNDLVLRDESFHNWEE
                     AEEDVYLGYVNAAMEGSARLAPRLNLSGRIEAGYIFSAQVENSLVSGRTLDGDDGLIL
                     GAQLSLDWQASERSVVSCGLAWELQEINGDTSEFYAVQQGNVYLYEVEWPDNTMESVW
                     AQINWMFSF"
     gene            7330..7716
                     /locus_tag="PKK36_02015"
     CDS             7330..7716
                     /locus_tag="PKK36_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93363.1"
                     /translation="MPRKKQSEDDLELRFLEALSRRSPDHVRILQALGDMYTQTGQIR
                     EGLAVDLRLSSLAPGDGTVWYNLACSYALSGSKDDAFAALEKAVFTGYAGYDWMQEDK
                     DLESIRKDPRFKQLLTRMKGSGRQEH"
     gene            complement(7717..8919)
                     /gene="purD"
                     /locus_tag="PKK36_02020"
     CDS             complement(7717..8919)
                     /gene="purD"
                     /locus_tag="PKK36_02020"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNR93364.1"
                     /translation="MRISVIGQGGREHALVRALHGNELFAWPGSDAIFELARPFPACD
                     LAALPAVMKKMRIELCIVGPEAYLDKGLADRCRAKGIPVLGPSQKDARLETDKVFAKR
                     FMARHRIPAAPYKIITDVEDIKAPCVLKFSRLAGGKGTCVCRTILEARRFAQKAMVEK
                     VFGDGQLIAESLLTGRELSVICTIAEGEYHILPPARDYKPLRDGDRGPNTGGMGTVAS
                     RDLEKNSMADIEALIIRPTVAALRHYRGFLYFGLMLTPDGPQVLEYNCRMGDPEAQAM
                     FPLIKGSLAEYFRQAAEGFINRELVRFSPGWTVCVTAAAGKYPQGIAESLFIQGLEKI
                     SGDIMIYHAGSRRAANGKWRTNGGRIIGLTAKSADLATAREHAYAALQYIQFKGMQFR
                     TDIGKLHF"
     gene            complement(8916..9458)
                     /gene="purN"
                     /locus_tag="PKK36_02025"
     CDS             complement(8916..9458)
                     /gene="purN"
                     /locus_tag="PKK36_02025"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNR93365.1"
                     /translation="MNIKLAVMASGRGSNFEALVHAARTGRLHAAICMLVCDQPNAPV
                     CSIARSYDIPVISFDEQTAGKIRAAGCNLIALAGFMRIVSPEFVRQFPGRILNIHPSL
                     LPRFRGLHAQRQALEHHETIAGCTVHVVNSEVDSGTVIAQRVVFIKPGDTEESLSARI
                     LKEEHKLYAEAIQHYSGWLS"
     gene            complement(9455..10420)
                     /gene="purM"
                     /locus_tag="PKK36_02030"
     CDS             complement(9455..10420)
                     /gene="purM"
                     /locus_tag="PKK36_02030"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNR93366.1"
                     /translation="MKKRFTYKQAGVDLDLAEQCVRDIGLLQKSTEGRFRLSNAFGQF
                     TANCDLSRYKAPVITATCDGIGTKLDLLLKHDLLETAGADLVAMNVNDVLTSSAEPAM
                     FLDYIAMPLMDRAIVRRLVSGMAEALQSCGCMLGGGELAEMPGTASIELSGFCVGVAE
                     KNDVPDISSIRPGDTVWGIPSDGFHANGFSLIRRILKEHPALLSRHETAELQKTTRIY
                     YPEVHMFKHQNIPVKGMAHVTGGGIVVNLNRILGEHGAELEIPYWDSPAAQKIISKVE
                     AGEAFRVFNMGIGWLMVNDGTDAPPPAIRLGHVTDDHTVRIKVGS"
     gene            complement(10417..11130)
                     /locus_tag="PKK36_02035"
     CDS             complement(10417..11130)
                     /locus_tag="PKK36_02035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNR93367.1"
                     /translation="MAERPQALVLKFPGTNCDRETADALKLAGFNVSVMPVTQFAKDS
                     LRTCRLLVLAGGFSYGDYVLAGKLAQLELECSAGNDLRSFTAQGGYILGICNGFQILL
                     HLGLLPDGALVENESGRFICKWAGLHKTGVSSPFLEGLPAEFELPVAHAEGRFVHRTR
                     IPESRIALRYSENVNGSMDSVAALQDATGRVLGIMPHPERFVRREQHYDRDWTGHPMW
                     GWGYFFFKSIHDHILNEAL"
     gene            complement(11100..13361)
                     /locus_tag="PKK36_02040"
     CDS             complement(11100..13361)
                     /locus_tag="PKK36_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNR93368.1"
                     /translation="MPDPVFRSMPLDGMTRIRLEELSRSMDLALDACDMCAIQDHFKS
                     GTRAPTDVELEVIAQTWSEHCKHRTFNAVVEHGAEILDGLFKTFIRDVSTRISAGKGD
                     FVLGAFEDNAGFIRLDEKFALCFKVETHNHPSAIEPYAGANTGLGGVIRDILGAGKGA
                     KPVANLDVFCFGRPELSDDSVPENVIHPRDTMKGVVRGVADYGNRMGIPTVSGAVYFH
                     EKFTHNPLVFCGTAGVIPVEDISKRVEPGMILVLAGGHTGRDGLHGATFSSLTLDEES
                     HDTDRGAVQIGNPIEEKKLTDFILQSRIKGLIESINDCGAGGISSAAGEILSRTGGTL
                     ILDDVHLKETGLKTWEILLSESQERMILVIRLENLEPLRELAAVYETTCSVIGKVEDN
                     GILQVVHGGETVCSLENSFLHHPPVKRLQSECRRPDSAAPTGSRKLKTLLADINICSR
                     EPVIRQYDHEVQGNTLIKPLAGAGGDCPQDGAVMQVRGSSKYFALGVSLTVHTGGDPF
                     AMGHAAVDEAVRQVLVTGANPDKIALLDNFCAGDPRNPRDLGPLVECLKGLSSAAGIF
                     QAPFISGKDSFNNTYQSPEGTESIPLTLLTSSIGIIDDSRHITGASIRRNDSLICLLG
                     AEYNPQQAVMNYRAFYEAVQAGCILSAHDISDGGLAVALAEAAFSMKAGVEADTVQDI
                     YAECPGRIIFEINAADREQVEEVFRNSQLSILGHTTAAHRRLVIRQRGQEVVNEPLEE
                     LKAIWQNALKPWC"
     gene            complement(13354..13656)
                     /locus_tag="PKK36_02045"
     CDS             complement(13354..13656)
                     /locus_tag="PKK36_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93369.1"
                     /translation="MQFLHRLLLNPQSEEMHVNAPPVIVPHVCCINIRMRPGHLDLER
                     EYILGYCRHITTEDVQIADIKPVQRIYLLGDRIPDMPRILRHLVNPVIHEWEVVYA"
     gene            complement(13791..16541)
                     /locus_tag="PKK36_02050"
     CDS             complement(13791..16541)
                     /locus_tag="PKK36_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNR93370.1"
                     /translation="MNSGSNLITDPVGDVLKYTGHPLDAIFQPKSVALIGATEKPNSV
                     GRTLLWNLISNPFGGVVYPVNPTRQSVMGIKAYPSIAAIPEKVDLAVFVTPAKLIPGL
                     VKECADAGVKGAVIISAGFKELGAPGIKLEQAVLENARSGGMRIIGPNCLGVMNPLIR
                     LNVTFGSTMAAPGNVAFLSQSGALCTAILDWARQKQVGFSSFVSTGSMLDVNWGDLID
                     YFGDDPRTQSIVIYMESIGNARAFLSAAREVAQNKPIIVIKAGRTAQAAQAAASHTGS
                     MTGSDDVLDAAFRRVGVLRVNSINELFDMSDVLAKQPLPKGKRLSILTNAGGPGVLAT
                     DALITGGGELAELSQSTFEALNAILPAAWSRNNPIDILGDAQPEKYAEALAIAAKDPH
                     ADGLLVILTPQDMTDPTQTAEKLKPYATIEGKPVFASWMGGAAVAAGIDILNKANIPT
                     YKYPDMATQAFNYMHKYSENLRALYQTPSMEEESDESPWPDREKAAAIISRVRREGRT
                     LLTEVESKELLGSYGIPTTPMKVAKTPAEAAKAAQEMGFPVVVKLHSTTITHKTDVGG
                     VLLNLRDAESVMNAFTRIQKSVAEKASAEGFEGVTVQPMIKLEGYEVILGSSLDPQFG
                     PVLLFGLGGQLVEVFKDRALGLPPLNANLARRMLENTKIYKALKGVRGRKSVDIDQLI
                     KIMVRFSQLVVEQPWIVESDINPLIASPDQLLALDGRFVLHGEKVSEKDLPKPAIRPY
                     PIQYISRWEMDDGAKVALRPVSPGDEKKMIAFHKTLSAQSVYLRYANRLDLQQRIAHD
                     RLVRVCFVDYDREMVLVADRKDPKTQERSIIALARLSKIHGSEEAKFSLLITDKYQKR
                     GLGTQIMQKLLAIGREEKMSTIVATILKDNKGMQDLCRKLGFTLTEEADSPFVKAEIS
                     LS"
     gene            16831..17673
                     /locus_tag="PKK36_02055"
     CDS             16831..17673
                     /locus_tag="PKK36_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93371.1"
                     /translation="MDELLWKLEHESCDKPVVMLGDSVGRQISLAMRGRGDTYFAPLA
                     TSAAVETAGQYFVFLRYLEHNRKPDRVILFMRNPLDGDLRKVCTENYVQRCFTRWREI
                     GSLTWQARDPAFGLRMLSYRLASAKYRLHLQRTIPFLPVKDSFEGIPEDQLLERKKSL
                     RKQSLKQRLISLLLPRSTETISEYYFLKLLDECRRRSIPLYFQPTPMRESKISTPEET
                     LRRKALFSRMGELAPDYPLFSYSDVVKSYPDRYFVDGIHIQQSKLSQVSEEYADLMDQ
                     IHAP"
     gene            17751..19169
                     /locus_tag="PKK36_02060"
     CDS             17751..19169
                     /locus_tag="PKK36_02060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNR93372.1"
                     /translation="MLFNSAEFIIFFVVLYGLYVVLPHRPQNLLLLAGSYIFYAAWDW
                     RFLSLILISTVIDYYCAHAIDRSDMPRRKKAFLTVSMVSNLGLLGIFKYSNFFVHSMI
                     SLLGRFGLEVQPLTLNIILPMGISFYTFQTMSYTIDVYRGQLKPAVNILDFALYVTLF
                     PQLVAGPIERASNLFPQVIRPRKISRYGITHGAYLIFWGYFQKVFIADNIAPLVEKLF
                     EGSAPYNGVHVLLGLYAFALQIYCDFAGYSNIARGLAFCMGFRFMLNFNLPYFATNPS
                     DFWARWHISLSTWLRDYLYIPLGGNRKGTWFTYRNLALTMILGGLWHGAAWNFVLWGI
                     YQGILLIGHRWYRPFVAPAVPWKAAALRVLHTVAFFHLVCLGWLLFRAESLHQIGAML
                     SALFTGFVWPSQLVLEHLVAAILFFGGLMMVIEWFQFRSSDLYVVLKWPLPVRVAVYL
                     LMFYGLVIFGVQDAQSFIYFQF"
     gene            19138..20103
                     /locus_tag="PKK36_02065"
     CDS             19138..20103
                     /locus_tag="PKK36_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93373.1"
                     /translation="MPNRLSIFSSRLLASGVAWTAAAVFCIESWCWKTLPSKYFSHEV
                     DILLYDLQTRTYDADTIVLGDSVGRQLSRYLAGQKKCDFIPLATNGAVEMAGQYYILI
                     RYLERNRPPSAVILLKHHVLHGDMKRVTTENYLQRCFTRWYEMLSMTWQSQSPSFGLR
                     MLSYRLAAAKYRLHLQKLIPGLPPPETGGNARQVEERLEVLEKTPPLQQLMKRLFPSD
                     LNSISEYYFIRLLSLCQQRNIRIYYIPCPMTNSTWTRYKCGVAYARLVRRLQELSEDF
                     PVLTYTTEIRDYPNGWFEDGIHISAEKLPVVADDYSRLMTELMRQ"
     gene            20250..21056
                     /locus_tag="PKK36_02070"
     CDS             20250..21056
                     /locus_tag="PKK36_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93374.1"
                     /translation="MQTRNYDAETLVLGDSVARQIALYLAAEGEADFLQLTTNGALQM
                     AGQYYILQRYLERNRAPENVILLKHRPLHGNLNRPATENYVQRCFTRWHEIAEMTRYT
                     GSPAFGLRALSYRMATAKYRLHLQELVPWLPAHAVVDDPERLNEPADGENPMRLPVRM
                     LSPGQRISVSERYFLKMIDLCSRNNIRLGYLPCPMPDEQWKDYQTGPAHARLVARLGE
                     ISQAYPGLRYITEIKTYPADWFIDGVHVRKARLPRVAADYAQMMNEMMQP"
     gene            21053..21940
                     /locus_tag="PKK36_02075"
     CDS             21053..21940
                     /locus_tag="PKK36_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNR93375.1"
                     /translation="MKIQQYLQREKQIHKAWAKLNKAADALESTRALVLLAGALSDLR
                     GDMPSGGIAEMAGQAGLTAAEQEQIEGLLTGEKDGADTGMHNIGALIALLWRIIREER
                     LHLMMNAPRGALFLRRMLQVALLAAGVFAAALLIRAGCYAVRLAAYNGCRVTYYRGTD
                     FNQKIGVSVEPALRAGYKRNRPFWYAPASKWSARWEADLKVPDDDVYSFFTQSDDGLR
                     LYIDDDLLVDSWKDQEWRTSGQHAEIRLSSGFHRIRVEHYFRSGSAAIVVKWAGGSVP
                     KNTVLEYPYLQKPGGRLHD"
     gene            21933..23513
                     /locus_tag="PKK36_02080"
     CDS             21933..23513
                     /locus_tag="PKK36_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93376.1"
                     /translation="MIKAELNRAVLIGACIIGVAFISVLAVGLCAREPMIGDEVIHYY
                     MLVNQAEQLPAPTVEAHIPLGTGKVYKRYYPHVFLWHYIGALFWKMAGGKFWGVQLYQ
                     SLFWLQYLVVGMLLAQQADPERKWGVPVYTALLASLPISLIFSVAFYQDVAATAQIMT
                     SFYLLRRGRALAAAVFLGIALALKVTVFIMLPAFIVCLIMFGRHSVSWPRLLVRAAAA
                     LMIIAALCWPMAKGLHYCGFAYYPTTMLQLYAKKLGLVKDMAVVRSVKPGDTEEHRVN
                     LPVTGARSVQEVVNHPGDLRITRNWLIYFGGVMWVVAAGGLLAVLRPGTKPLRMAGGW
                     ALAVGAWYILITALHMCNSPDARFFLPGVVLAMLPFALMFSRWPMARIWVPLMLAVSL
                     AQSGVVLAKTHQLRRISPELQQTVEFLKDNPPVPHGVFMYPEGNYRLFPVRHDWYLGF
                     RLKEFWMADNDVRIQMLRSADLGAIVIKKHLIGRLDDKMSNLGIYPDFFVRDISADSR
                     FVRVFENSGFTVYSVPAE"
     gene            23601..24356
                     /locus_tag="PKK36_02085"
     CDS             23601..24356
                     /locus_tag="PKK36_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93377.1"
                     /translation="MKETIVIVPAYNEELNIKTLLDEVRSAGPDLDVVILNDGSLDRT
                     AEAARRSGAKVIDLPCNLGVGGAVQAGFSYAYENGYQYAVRIDGDGQHPPSEIPRLLE
                     AIKSSEADLILGSRFLGEKSYGSTRLRYCGIYLLAQFLSLICRRKVTDPTSGFQALRR
                     PLMYFFSQAYPMDYPEPEALALMRRQGYDFAEVPAVFRERQAGVSSIQGWGTLYYMLK
                     VFLALIVDRARSVDPRLSRQRVMEAMQHGRDGY"
     gene            24346..24915
                     /locus_tag="PKK36_02090"
     CDS             24346..24915
                     /locus_tag="PKK36_02090"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNR93378.1"
                     /translation="MDINAYLTGWQTAIVPRILIGLLGLLIVLILARTIRSKRISLVP
                     AILWSLAAAVLIIFSLFPQQLIRTVISTEYVMRMRIMMGFVSLLVLLITLESTRQARL
                     QERYALLWVATALVILICAFFPSVVNLLRLLMGMNYTAAVVSVAFTFLTLVLFHFSIS
                     MSISQAKQARIAQKLALLETRVRELEERR"
     gene            complement(24920..27253)
                     /locus_tag="PKK36_02095"
     CDS             complement(24920..27253)
                     /locus_tag="PKK36_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93379.1"
                     /translation="MMAVTIYSLLSLGLLAGALFFLLRPVIRMFYAKLKGGGGILAAL
                     LLLSGSILLLRPHQEALTGLDDSAYRLMAQAMHEGRPLFETDELLLQVPEPHRAAFLF
                     RPNMTGHGRLTRDQSYALDDLTSCMTRPFYYPLLPFTANGLDLIIPGPAADGLIPVLG
                     ILTLLTLMCLGIARGRWPGMLLAWVLFFSTPLPVWIFRGWYSEAVAGSLLCLSLVNLL
                     LDKNTIPRRALCFALGLMISYHVSFLVIALPLLIAVLMLPPDGNRRISSAALFFPAGL
                     LPLFWQTQFICKPYGGLAVGSLLSFFRLGATHWLLMVYLAAILAAGSLLLLFRRRTLM
                     LLSTVLESGVLPRLGAVCAAAIPLFCSLVFWHQKHVVAQGLLDAHSAMGLHLELLLGI
                     LLLAVLLHRRQHAGLLILLLALLVLPVNLYLKGAELVGLWSQRRLLGTLMLLLPPAVC
                     GGGVIADWTAGRLKGRPLRLAAGAGVITVLVLGLLNAVRWPAPWLLRNEKGSRAYVQS
                     MRDVIGSDLAIIDYHPWSIPLAAVPGSRVLGIGPGRVHDAGAAIPHLMAQADGRRVLF
                     LSAYETHAVLEQDAVLVPVQTYQFHLPHIHSKRALPAVEKEYPMTLTLLKSRRLNDSE
                     WTRRYTRVMGSGVLGFRGPWGRTDIPLKDTAGLNLPARWSREGSGLIGPMPLQGEAVT
                     LTLTCAANRQDGKTQTLLIMPPWSDEAAFLDISNSICTVSVSLTRPPDRLMSGETGNY
                     RIYSVHPYNPARDGIRGFKKDLGALIHSVTFEVQSQD"
     gene            complement(27250..28371)
                     /locus_tag="PKK36_02100"
     CDS             complement(27250..28371)
                     /locus_tag="PKK36_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR93380.1"
                     /translation="MHIGVNTLFLIPGEVGGSETYLCRILDGIAAGYPSERITLFTSN
                     ENDACLKGRYQMFSQFAFKRIPCTASRRSERILKEQILLPRAVNISGADVLWSPGYTA
                     CLFSPVPQVVTIYDMQYKTFPEDLTLPARIATDVLVRLAVRRCEGILTISNFSKQEIL
                     KYTAARPERIIVTHLASHPHHPADGPNPAGDKPYILSVANSYPHKNLHQLVDAFVMLE
                     DRIPHNLVIVGRARLGEERLRQAINRLKNPGRVLRLSGVSSDALDSLYAAAEVFVFPS
                     LYEGFGLPVLEAMSAGTPVITTRKGSLPEAGGQAAMYYDPADTSELAKVILNALNQPH
                     AEREDVARRGRAHAGSFSWQATSDKTLAALRRAAETHAV"
     gene            complement(28375..29169)
                     /locus_tag="PKK36_02105"
     CDS             complement(28375..29169)
                     /locus_tag="PKK36_02105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93381.1"
                     /translation="MKISVITACRNSEAFIERTIQSVIRQRGAWGELEYLVMDGASTD
                     GTQDILRKYAGDLDEVVSEQDAGPASAINKGLRRATGDILCWLNADDVYSPGTLERVT
                     AAFAKHPKAALVFGRCTIIDEHDREIRRGITGFKHMFFPFSCRFMIQCINYVSQPAMF
                     FRRSAFEKAGPLSESLKAAWDYEFMLRLWKHGGAAVVAPPALAAFRWHEQSISGQHFH
                     MQFKEEFDAAVKDAGRVSPQALAHLFVRWGIVGAYTLMGKARKAGN"
     gene            29278..>29737
                     /locus_tag="PKK36_02110"
     CDS             29278..>29737
                     /locus_tag="PKK36_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR93382.1"
                     /translation="MSFWEKKNVLVTGGAGFLGSHVVDELKRMGCKNIFIPRSAQYDL
                     REKGDIQRCLKDSNPDLVIHLAAVVGGIGANREHPGSFLYDNAIMGISLIEECRKFGV
                     AKMTLLGTICAYPKITPIPFREENLWNGYPEETNAPYGLAKKLMLVQSQAY"
BASE COUNT         6177 a         8162 c         8719 g         6679 t
ORIGIN      
        1 tggcggggca cggggctgga ggactacttc aacggcggat ggtattacca gaacttcctt
       61 gcgcggccgc tgaacgggat tgtcttcaag gctccgttcc gcgcggtgca gtatcggatt
      121 cactttgcgg atccggtcat gtttcacagc gcctttgaga tgaactttga acgcggcccg
      181 gagatggcca gccgcggcgt gtatgagagt gtggcgtatt actatctgaa gaccccgcag
      241 aggtcgccct cggagattgc acaggatgct tcccgctact ttcccgatga tccgatggcc
      301 cgggccacgt acatgaccga ggtcaacaat atcgaacgaa tggatgatta tgcgggcgca
      361 gcggaataca cggaatattt tctggagaag aatccggaat tcccgtttgc agagacgctg
      421 cgcatgcggc gggatgccta ccgcgagaag atccgcggat cttccgctgc cccggagttt
      481 tacaagaagt atttgagctc atcgaatgaa gtcgcgcgga agcaggccgg ggacctggag
      541 tggttttatg ctgacccgtc ccgcgcgctg ctgggactgt actgcaatgc gccatcgcgt
      601 gcatttcttg acgggaaatt cgtgctgggg gcggggcgcg acgatcgctg cgagatcgcg
      661 cggctgtcgc ttccggacgg ccggcacacg ttcgccgtgc aggctcaatg gaagcagtat
      721 cccgactgga tgcagttctg cctcaggacc cattcgggtg atgtggtctc ccggccggac
      781 agcaaatatg cgctcgatgc agccggcggc tggatgaacc cggattatga tgacaacgcc
      841 tggccgcgtc tttccggaac gggtgtcaag ggcccccccg aggcgcccta tgtgtggatg
      901 gagcctcatc cgcacatcgg catgcactcc ggtgccgcgg gcctgcgccc ggaatggaac
      961 aaccgggttc agagttatgt ggtgtaccgg agcgaatttg acgtgccaag cggcaggtga
     1021 gccgctatat aatggctgcg gaatacggaa cgggactgag caatgaaaat gtggaagatg
     1081 cttctttttg cgggatgtgc atgggcttcg gtttgtgcct tttcgggctg ttcgcgcaat
     1141 gtggccaaca ttgatgagca ggaagagcgg catccgctga tgcagcgggc attgaatcac
     1201 aaacggctgc aggatattga caaggccata gaccttttcg aggagatcct ccgcgagaat
     1261 tccgacatgg cccgggcaca tctggaactg ggactcctat acgacagcta tcgggaagac
     1321 tacatgcgcg cgctttatca ttatcagcgc tatcttgaac ttcggcccga caccgaaaag
     1381 cgcgagatga ttgagaacat catgaaaatg gcgcgtctgt cttttgcggc aacgcttccg
     1441 cacagtcctt ccgacacgct ggatgagatt gcggctctca aacgggaaaa tgcaatgttg
     1501 accacccgga tcaacgagat gagccgtgcg ctgaatctgg cggaaaagaa ggccgccgag
     1561 gccgcccggc gggaggagtc agcgctgctg gcgcgtgtgc ctgatccgcc tgcccggctg
     1621 gctgacaccc gaccgcccga tgtccaggcc gaccgtccgc ccatggatgt ttcgccggac
     1681 cggattccga agcccgcccc cgcagcggca ccggcgcgtc cgcaatctcg gccggcgccg
     1741 gccgcggcgg tgaaacctcc gcctccggct ccgccggtcg cgcagaccta ccgggtgctg
     1801 cggggagata cactttccag cattgccgcc aagatgtata acaatcccca aatgtggacg
     1861 aaaatatttg atgccaaccg ggacaagctg gaccgtcccg agggaatccg cgtggggcag
     1921 gtgctggtga ttccataatt taacagcagg aggatacagt catgggagct actgattttt
     1981 ttgatgatga tctggtgaaa ccgggccgga aagagggcag catcagcatg gggccgggag
     2041 cgggcagtac caagtcggtt gagccgggag ccccttccac cacatttccg tcccgaccgg
     2101 tgtccgattt caacctgacg cgcatggcgc ggcacaagga cgaggttgaa acacaggtgg
     2161 cgcgcgccgc cgaagagctg gaacggctgc gccagcggca ggaggacctc gagcgcgaga
     2221 agcatgatct ggaggatctg cggcggatgc aggatgagta tcagcagggc cggcgggaaa
     2281 tgattgaaca gctcagtcag aacctgctga aacttgaaaa agatgaactt cgcgccgagc
     2341 agacgcgcga actgctggcc tccacccgca ggagtttcaa ggctatgctt gagaacatca
     2401 aggggctgaa tgatgaaacg tggacggacg aacagttccg cgaggagctt ggcaaggcgc
     2461 gcacggtcat tgaggatgcc cgcattgaat tcaacaaggc ccgcgcaaag attgacgcgc
     2521 tgatcagcat tgaggagaaa gctgaccgcg gacgtccggt ggtttttgag agcccgctcc
     2581 gcgaggagaa tgtgttttcc ttcggccact ggttcaaggt gggtatggcg gttacgctcc
     2641 ccctgacgat tctgatcgtt ctgctcatgg ctgcatttgc cgcgctgcga tatatgcggg
     2701 tgctgtagaa cggagccgct gcgcaatggg taaaggcaaa tcagcgcttg atcgccggct
     2761 ggagcagctg gagagggagg ccgcttccat ccagagcaat atcagcagcc tggattatgc
     2821 gctcaggcgc gccgaccggg gcgctccggc acggctgaaa ctgatgccgg acaggagatc
     2881 gttatctgcg catccggcgc ccgaaagaaa aacaaaagat ccggtggaga aaccggttcg
     2941 ccggaaaatc gcaaattatc tgtcaggcgg aagcttcagg gcggtaaagc tcactccgca
     3001 ggaaatggcg gttcagcgca acaaggccat tttcatgatc attgtgctga tcattgtgat
     3061 ttatgtcatg ctgaaacttt tccacaaggt gtgattgtgc cctccggccc gttcgcggcc
     3121 ttattacgga agaaccatcg cgatgatttc tgcacggata tcgcgtccaa tgtgaattcg
     3181 cgccagggtt ggccccgcat ccgaccgcgg ctgactgatg ctcccggggt tcacaaatag
     3241 tatgccttcg cgagaactga tggagggccg gtgcgtatga ccatgcagta caagcgctgc
     3301 cttcatcgcc ctgaaatcga cgtctgcagc cgcaagatca tgcaggaggc agaccgtatg
     3361 gcctccgagt tcaagcactt caacagagga gagccggccg gcccatgaat gccggtccat
     3421 gttcccgcgc acggcatgca caggcgctat tttactgagc tgatccagaa tggcgggaga
     3481 gccgatatca cccgcgtgaa aaatggagtc cgcgccgtgc agcatctgga gcgcgccatc
     3541 gggaagatgt ccgtgcgtat cggaaagcgc gaaagcaatg atttcttttt ctgctgacat
     3601 gacggatcaa ctatagttag catcggcagc attggcaacc gtaccggatg ggattatgtc
     3661 gtcgcctgtt ggacattcag tgattggtgc agcgtttggc gtgctgtatt ttctgaagcc
     3721 cgccggcagg cggggtcttt gggtccaggc ggcagagaag tggccctgga ttctgggtgc
     3781 ggcgcttttt gccaatttgc cggatattga ctacatcccc ggagtcatgg cgggggagct
     3841 gaatctttat catcatggag tcacacacac gctgggctgg gcggcgctgg tttcactgct
     3901 cgtatggatt ccgctgcggc ccaaaggggc ccggggcagg gaactgagtc tgatatttct
     3961 gctgcttttc agccatctcg cactggactg ggtgacggct gacgggaagg aaccctatgg
     4021 cataatgatc ctgtggccat tctcgaataa ttatttcatt tctgaagttc ctctgcttcc
     4081 tcatctgcgc aaggccgatt tcactgaact gctggatccg tacaatttca ggataatggc
     4141 cgtcgagatt ctgcggactc ttccggttct tctgctggcg ctggcgtgta aattatttgg
     4201 agggcggcgc ggcgcgccgc cgctgtgaaa cggtcccgca gagcccgccc ctccattcat
     4261 ccatcctgca agttgatctg ccgcaaagct tcgtagagca caatggcagc cgcattggcc
     4321 agattctgcg accgcacacg gatatctttc atggggagac agaccagttc ttcgggatgg
     4381 gcatccgtga tttcgggagg cagccccctg gtttcactcc cgaacaccag cgcatcgccc
     4441 gcctgatagg ccatatcggt ataaagtcgc ggcccgcggg tgctgaagaa gaagatcctt
     4501 tccgggttga tatccttctg aaatgcctga aatgaccggt ggcggaatat acgggcgtat
     4561 tcccagtaat ccagtcccgc gcgccgaatt gccgattcgg tcagctgaaa tccgagaggt
     4621 tcaatcaggt gcagttcaga accggtgccg gcgcaaagac ggacgatatt gcctgtattg
     4681 ggaggaatct ccggttccac cagaaccagc cgaagcgggg gggatggcca tcggaatcct
     4741 gttatgtggc atgatttttc tgacatcttg attgcaccgg gattttgcgg ccttttttgc
     4801 ttgcgccacc tccagatgcc ctctatattt cgcgcacttt ttattgagga gcttgaaatg
     4861 tcaagaatat gtgaagtatg tgggaaaggg ccgagaaccg gcaacaccat tgtcaggcgc
     4921 gggctggcta agagcaaggg cgggattggc ttgcacacaa ccggcataac gcgccggcgc
     4981 tatctgccca atctgcagaa gatccgtgtg gtggaaaatg gcggggtcaa gacccgcagg
     5041 gtttgcacca gctgcatcaa ggccggcaag gtttcaaagg tctgacgccg gatttccgct
     5101 cacgactgat gtgagccttc tccccggcac atccggggtc gtggtgttat atataaggag
     5161 attgccccat gagtgattcc aaagcacagt tggcccgact ggaaatcaac ggcaggaaat
     5221 gtgaatttcc cgttttgacc ggaagtgagg gggaacagag tgtggacatc cgccggctcc
     5281 ggaaggaaac cggactgacc acatttgatc cgggcttcgt caacaccgcc tcctgtcaga
     5341 gtgccatcac gtatctggac ggggaaaaag gcatcctgcg ctatcgcggg tatccggtcg
     5401 aagagctgat caaaatctgc aatttcattg atgtggccta tctcctcatt catggcgtac
     5461 tgcccaacga acaagttcgc aacgatttcg cgcgcctgct gaaccgccac tccatgattc
     5521 acgagtccat gcggacgttc tttgatcatt tcccggaaaa cgcccacccg atggcaattc
     5581 tttccgccat ggtggtttcg ctttccagtt tctatccgga aatggaaagc gatcaggaag
     5641 gggtggacat ggctgccacg cggcttctgt ccaagattcg cactattgcg gcgttttcct
     5701 acaaaaagtc catcggggag ccggtggtgc agcccagtta caagctttcg tactgtgaaa
     5761 acttcctgaa catgatgttc gcttcgcccg tggccaatta caaactcgac cccgttgttg
     5821 ttcgcgcact cgaccagctg ctgatcctgc acgcggatca tgagcagaac tgctccactt
     5881 cggctgtccg tctgatcgga agttccggcg ccaatcttta tgcctcagtg tccgccggca
     5941 tttgtgctct ctggggacct gcgcacggcg gagccaatca gggggtgatt cagatgcttg
     6001 agcgtctgca ggcggaggga ggcgacgcga agaagatcat tgatcgcgcc aaggatccga
     6061 atgccaattt ccgcctgatg ggattcggac accgcgtgta caaaacctat gacccgcgcg
     6121 cgcgaatagc caagcagctt tgcgaggagg tcctcggcaa actgggcata aatgagccgc
     6181 tgctggatct ggccatgaaa ctggagcagg cggccctttc cgatgaatac ttcatcgagc
     6241 gaaatcttta tcccaatgtg gacttctata ccgggatcat cttccgcgcc atgggcattc
     6301 ccaccgatat gttcacggtg atgttcgcca tcggccggct gccgggctgg atagccaact
     6361 ggcttgaatg gatgtccgat ccggcccaga aaataggccg tccgcgccag ctgtatgtcg
     6421 ggcccaaaaa gcagaaggta ccgccgcgca agcagggggg attctgattc ccgtgcgcag
     6481 tgcaatgacg cgcaccggga ggcagcattt cctttcgtgg atattgttca tcactgctcc
     6541 gctgctggcc gtttcttccg agccatggtc actggaggca gatattgcag cagggggcgg
     6601 gcggctctca tacgaagagg attccgcggg gctcgaatct gaatggtccg ctccggcatt
     6661 tatcctcgag ggccgggcgc ggcttcgcgc accccgccgt ctgctgtttg ccgcttccag
     6721 ccgcctgacc gtgacggata aggatcagga agaatggagc gaagacggcc ttgttcgaca
     6781 ggaaaaccag ctgcatctgg cgggagttca tgcgctgggt gaagcaggct gggccgttgt
     6841 gcaggccgag gctctgcagg cagcgattct gctgggtatg ggtgcgcgtt atcagcactt
     6901 tgtgcgcaat gacctggttc tgcgtgatga aagctttcat aactgggagg aggctgaaga
     6961 agacgtctat ctggggtatg tgaacgccgc catggagggc tctgcccggc tcgcgccacg
     7021 gctgaatctc agcggacgca ttgaggccgg atatatattc tccgcacagg tggaaaacag
     7081 tctcgtgagc gggcgcactt tggacggaga cgatggcctg attctcgggg cgcagctgtc
     7141 gctggactgg caggcctcag aacgcagcgt tgtgtcctgt ggactggcgt gggagctgca
     7201 ggaaatcaac ggcgatactt ccgagttcta tgctgtgcag cagggaaatg tttacctcta
     7261 tgaagttgaa tggccggaca acaccatgga gtctgtctgg gcgcagataa actggatgtt
     7321 ttctttctaa tgccccggaa gaaacaatcc gaagatgatt tggaactgcg cttcctggag
     7381 gccctttccc ggcgatcgcc ggatcatgtc cggattctgc aggcgctggg tgatatgtac
     7441 acgcagactg gccagatcag ggagggactg gcggttgatc ttcgactgag cagtctggcc
     7501 cccggggatg gtactgtctg gtacaatctt gcctgcagct atgcgctgag cggcagcaag
     7561 gacgatgcgt tcgcggcgct cgaaaaagcc gtttttacgg gctatgccgg ctatgactgg
     7621 atgcaggagg acaaggatct tgagtccatt cgcaaagatc cgcggttcaa gcagcttctg
     7681 accagaatga agggatcagg gcggcaggaa cactgactaa aaatgcagtt tcccgatatc
     7741 cgtgcgaaac tgcatcccct tgaattgaat gtattgaagc gctgcgtatg cgtgctcgcg
     7801 cgccgtggcc agatccgcgg attttgcggt aagaccgatt attcggcccc cgtttgtgcg
     7861 ccatttgccg tttgcggctc tgcggctacc cgcatgataa atcatgatgt cgccggagat
     7921 tttctcaagc ccctgaatga acaggctttc tgcgattccc tgcggatatt tccccgcagc
     7981 ggctgtgacg cagaccgtcc atccgggtga gaaccggaca agctccctgt tgatgaagcc
     8041 ttcggccgcc tgccgaaaat attccgccag gctgcctttg atcagcggaa acatggcctg
     8101 cgcttccgga tcgcccatgc ggcagttata ttcaaggacc tgcggcccgt ccggcgtcag
     8161 catgaggcca aaatagagga acccgcgata atgcctgagc gccgcgaccg tcggcctgat
     8221 gatcagtgct tcgatatcag ccatgctgtt cttttcaagg tcgcgcgaag ccacggtgcc
     8281 catgccgccc gtgttcgggc cgcggtcacc gtcgcgcagg ggtttataat cgcgggcagg
     8341 aggcagaata tgatattcgc cttcagcgat ggtgcagatt acagaaagct ctctcccggt
     8401 cagcagactt tccgcaatta gttgtccgtc tccaaaaacc ttctcgacca tcgccttttg
     8461 ggcgaatcgg cgggcttcaa gtattgtacg gcacacacag gtgcctttgc cgcccgccag
     8521 ccggctgaac ttcagcacgc agggggcctt gatatcttcg acatcggtta tgattttgta
     8581 tggcgcagcg ggaatgcgat gccgggccat gaatcttttt gcgaacacct tgtcggtttc
     8641 aaggcgggcg tctttttgtg aggggcctag aaccggtatg cctttagcac gacagcggtc
     8701 agccaatccc ttatcaagat atgcctcagg tcccacaatg cacagttcga tgcgcatctt
     8761 tttcatcacg gcgggaagtg cggcaagatc gcaggcgggg aaggggcggg ccagttcaaa
     8821 aatcgcgtcg ctgccgggcc atgcgaacag ttcattcccg tggagagccc tcacaagcgc
     8881 atgttcgcgg cctccctgcc cgatgacgga gattctcatg acagccaccc ggaataatgc
     8941 tgaatggctt cggcataaag cttgtgctcc tccttcagaa tccgcgccga caggctctct
     9001 tcagtatcgc cgggcttgat gaaaacgacg cgctgagcga tgaccgttcc agaatccact
     9061 tcactgttca caacatggac tgtgcagccg gcaattgttt catgatgttc gagtgcctgc
     9121 cgttgagcat gcagccccct gaaacgcgga agcagtgacg gatgaatgtt gaggatgcgc
     9181 cccgggaact gacggacgaa ttcggggctt acaatccgca tgaatccagc cagcgcaatc
     9241 agattgcagc ccgcggcacg aatcttcccg gccgtttgtt cgtcaaaaga aatgaccgga
     9301 atatcgtatg atctggcgat gctgcatacc ggcgcattgg gctgatcgca gaccagcatg
     9361 cagatggctg catgcagcct tcccgtccgg gccgcgtgaa caagtgcctc gaaattgctt
     9421 cctcgtccgg aagccatgac cgcaagtttt atgttcatga ccccaccttg atccgcacgg
     9481 tatgatcatc tgttacgtgt ccaagccgga tcgcgggagg gggggcgtct gttccgtcgt
     9541 ttaccatcag ccatccgatg cccatgttga aaactctgaa ggcttcgcct gcctcgactt
     9601 ttgaaatgat cttttgtgcg gcggggctgt cccagtatgg aatttcaagt tccgcgccat
     9661 gttcgccaag aatgcggttc agattcacca cgatgccccc accggtgaca tgagccatgc
     9721 ctttaaccgg gatattctga tgcttgaaca tgtgaacttc cgggtagtaa atccgggttg
     9781 ttttctgcag ctctgctgtc tcgtgacggc ttaaaagtgc cggatgctcc ttgagaatac
     9841 gccggatcag gctgaacccg ttggcgtgga agccgtccga cggaatgccc cataccgtgt
     9901 ctccgggccg gatggaggat atgtccggca catcattttt ctccgcaaca cccacgcaaa
     9961 aaccggagag ctcaattgaa gccgtgcccg gcatttctgc cagttctccg ccgccaagca
    10021 tgcagccgca ggattgcagg gcctcggcca tgccgctgac cagccgccgt acaatggcgc
    10081 gatccatcaa gggcatggcg atataatcca gaaacatggc cggttctgcg cttgaggtca
    10141 gcacatcatt gacgttcatg gcgacgaggt cggcgcccgc ggtttccagc agatcatgct
    10201 tcagcagaag atccagcttc gttccaatgc cgtcgcaggt tgctgtaata actggggctt
    10261 tatagcgtga cagatcacag ttggccgtga actggccgaa ggcattgctc agccggaaac
    10321 gtccctccgt gcttttctga agcaggccaa tgtcccgaac acattgttcc gcaagatcca
    10381 ggtcaacgcc cgcctgttta tatgtgaagc gttttttcac aatgcctcat ttaaaatgtg
    10441 atcatggatg gatttaaaga aaaaatatcc ccatccccac atgggatgtc cagtccagtc
    10501 gcggtcgtaa tgctgttcgc gccggacaaa gcgttcgggg tggggcatga tgcccagcac
    10561 gcggccggtg gcgtcctgca gggcggcaac ggaatccatg gagccattca cattttcaga
    10621 gtaccgcagg gcgatgcggg attcggggat tcgagtcctg tgtacaaacc gcccttcagc
    10681 gtgagccacg ggcagttcaa actcagccgg gagaccttca agaaacgggc tggatacgcc
    10741 ggttttgtgg aggccagccc atttgcagat gaaccgtccg gattcatttt cgaccagtgc
    10801 cccatccggc aggaggccga gatgcagcag tatctggaat ccgttgcaga ttccgagaat
    10861 ataaccgccc tgtgccgtga agctgcgcaa atcattaccg gcggagcatt cgagttccag
    10921 ctgggcgagt ttcccggcga gcacatagtc cccgtaactg aatccgccgg ccagcaccag
    10981 aagccggcag gtgcgaagcg aatcctttgc gaactgagtt acaggcataa cagatacatt
    11041 gaatcccgca agcttcagtg catcggctgt ttctctgtca cagttggtgc cggggaattt
    11101 cagcaccagg gcttgagggc gttctgccat atagccttga gttcctccaa aggttcattc
    11161 acaacttcct ggccgcgctg acgaatcacc agcctgcggt gtgcggcggt tgtgtgcccc
    11221 agtattgaga gctgactgtt tctgaatact tcttcgacct gttcgcggtc tgcagcgtta
    11281 atttcaaaaa tgattcggcc tgggcattcg gcataaatat cctgaacggt atccgcttca
    11341 actcccgctt tcattgaaaa agccgcttcc gccagcgcca ccgccagccc gccgtcggat
    11401 atgtcatggg cagagagtat acaacccgcc tgcacagctt cataaaaagc ccggtaattc
    11461 atgaccgcct gctgtggatt atattcggcg cccagcaggc agatcaggct gtcattgcgg
    11521 cgtatggatg ccccggtgat atgccgtgaa tcgtctatga ttccgatgga gcttgtaaga
    11581 agcgtgagag gaatggactc ggttccttcc ggggattgat aagtgttgtt aaatgaatct
    11641 tttcccgaga tgaatggggc ctggaagatt cctgcggcgg acgagagccc cttcaggcat
    11701 tcaacgagcg gtccgaggtc gcgcgggttc cggggatcgc ctgcgcagaa gttgtccaga
    11761 agcgcaatct tgtccggatt cgcgccagtc acgagaacct gcctgacggc ctcgtccacg
    11821 gcggcgtgtc ccatcgcaaa ggggtcgccg ccggtgtgaa cagtcaggga aaccccgagc
    11881 gcgaaatatt tggaacttcc ccgcacctgc atcaccgccc cgtcctgcgg gcagtcgccg
    11941 cccgcccccg ccagcggctt gatcagggtg ttgccctgca cctcgtggtc atattgccgg
    12001 atcacaggtt cacgagagca tatgttgatg tcggccagca atgttttcag cttccggctg
    12061 cctgtgggag ctgccgaatc gggccgcctg cattctgact gaagccgttt taccggagga
    12121 tgatgaagaa aagagttctc gagcgaacaa acggtttctc ccccgtgcac gacctgaaga
    12181 attccattgt cttccacttt tccgattaca ctgcaggtgg tctcatatac ggcggccagt
    12241 tcacgcagtg gttcaaggtt ttccaggcgg atgacgagaa tcatacgttc ctggctttcg
    12301 gagagaagga tttcccatgt tttcaggccg gtttccttta gatgaacatc gtccaaaatg
    12361 agagttccgc ctgtgcggga aagaatctct ccggccgcgc tggatatgcc gcccgcgccg
    12421 cagtcgttta tgctttcgat aaggcccttg atccgcgatt gcaggatgaa atcagtaagt
    12481 ttcttctcct caatgggatt tccgatctgc acggccccgc gatcagtgtc atggctctct
    12541 tcatccagcg tgagcgacga aaacgtcgcg ccgtgcagtc cgtcccggcc ggtatgtccg
    12601 ccggccagca caagaatcat gccgggctca acgcgtttcg aaatgtcctc aacaggaatt
    12661 actcccgctg tgccgcagaa gaccagcggg ttgtgtgtga acttttcatg aaaataaacg
    12721 gccccgctga cggtggggat ccccatgcgg ttcccgtagt ccgccacacc gcgcacaacg
    12781 cctttcatgg tgtcgcgcgg atggatcacg ttttccggaa ctgaatcatc actgagttcc
    12841 ggccgcccga agcagaatac atcgagattg gccaccggct ttgcgccttt gcccgcgcca
    12901 aggatgtcgc ggatgacccc gcccaggccg gtgttggccc ccgcataggg ttcaatggca
    12961 gacggatgat tatgagtttc gaccttgaaa cagagcgcaa acttctcatc gaggcggatg
    13021 aaaccggcat tgtcttcaaa tgcacccagc acgaaatccc ctttgcccgc ggagatacgg
    13081 gtggaaacat cccgaatgaa tgtcttgaaa aggccgtcaa ggatctcggc gccgtgttca
    13141 acgacagcat tgaaggtccg gtgcttgcag tgctcgctcc atgtttgcgc gatcacctcg
    13201 agttcaacgt ccgtcggtgc gcgtgtaccc gatttgaagt ggtcctgaat ggcgcacatg
    13261 tcacatgcat ccagcgcaag gtccatggac cggctcagtt cctccaaccg gatgcgggtc
    13321 atgccatcca gcggcatgct gcggaagacg gggtcaggca taaaccacct cccactcgtg
    13381 gatgacggga ttcacgagat gccggaggat ccgtggcatg tcggggatgc ggtctccgag
    13441 cagataaatc cgctggacgg gtttgatgtc agcgatttga acatcctctg tggtgatgtg
    13501 ccggcaatat cccagaatgt attcccgctc caggtcgaga tgcccggggc gcatgcggat
    13561 attgatgcag cacacatgcg ggactatcac gggcggggcg ttgacatgca tctcttccga
    13621 ctgcgggttc agcagcagcc gatgaagaaa ctgcacggcg gaatcccgag ccccctcgaa
    13681 atcacaggtg tagcaccgcg tcttcctcca tgtgaaatcc gcgcctgcta tgagttcgtt
    13741 tcctttgcgt ggctgaacaa acaggctgtc tatttcgagg tgcagtgtat tcagcttagg
    13801 gatatctccg ccttcacaaa cggtgagtcg gcttcttcgg tgagcgtgaa gcccagtttc
    13861 cggcacagat cctgcatgcc cttgttgtcc ttcaggatcg tggcgacaat cgtgctcatc
    13921 ttctcctcgc gtccgatggc cagcagcttc tgcatgattt gtgtgcccag cccgcgcttt
    13981 tggtatttgt cagtgatgag cagcgaaaac ttggcttctt cagagccgtg aattttgctc
    14041 aatcgggcca gcgcgatgat cgagcgctcc tgggtctttg gatccttgcg gtcggccacc
    14101 agcaccattt cgcggtcgta atccacaaaa cagacacgaa ccagccggtc atgcgcgatg
    14161 cgctgctgga gatccagccg gttggcgtag cgcagataga cactctgggc ggaaagcgtc
    14221 ttgtgaaagg caatcatttt cttttcgtcg cccggactca caggccgcag ggctactttt
    14281 gcgccgtcat ccatctccca ccggctgata tactggatgg ggtaggggcg gatggccggt
    14341 ttcgggaggt ccttttccga aaccttttcg ccatgcagca cgaagcgtcc atccagcgcc
    14401 agaagctgat cgggagatgc gatcagggga ttgatgtcgc tttccacgat ccatggctgt
    14461 tcaaccacca gctgactgaa gcggaccatg attttgatga gctggtcgat atccacgctc
    14521 ttgcgtccgc ggacgccttt gagcgccttg taaatcttgg tgttttccag catgcgccgc
    14581 gccagattgg cgttcagcgg ggggaggccc agagcccggt ccttgaacac ctccacgagc
    14641 tgtccgccca ggccaaaaag aagcaccggg ccgaactggg gatccagcga gctgccgaga
    14701 ataacctcgt agccttccag tttgatcatg ggctggacgg tcacgccttc aaaaccctct
    14761 gcactggcct tttcggccac ggatttctga attcgggtga aggcattcat tacgctttcc
    14821 gcatcgcgca gattgaggag cacccctccc acatcggttt tgtgcgtgat cgttgtcgaa
    14881 tgcagcttga ccaccacggg aaaccccatc tcctgtgcag ccttggcggc ctcggccgga
    14941 gtctttgcga ctttcatcgg cgtggtcggg atgccatagg agcccagcag ctccttggac
    15001 tcgacctcgg tcaggagtgt tctgccttcc ctgcgcaccc ggctgatgat agcggcggct
    15061 ttttcgcggt ccggccatgg gctttcatcg ctttcctcct ccatggaggg ggtctggtaa
    15121 agcgcccgca ggttctcgct gtatttgtgc atgtagttga acgcctgcgt ggccatatcc
    15181 ggatatttgt aggtcgggat attggccttg ttcagaatat caatgccggc cgcgacggcg
    15241 gcgccgccca tccagcttgc aaagacgggc ttgccctcga tcgtggcata aggcttgagt
    15301 ttctcagccg tctgagtggg atcggtcatg tcctgcgggg tcaggataac cagcagaccg
    15361 tccgcatggg gatccttggc ggcgatggcc agcgcctcgg catatttttc aggttgggcg
    15421 tctcccagaa tgtcgatcgg attgttccgg ctccaggcgg caggaaggat ggcattcaga
    15481 gcctcaaacg tgctctggga aagctccgcc agctcgccgc cgccggtaat cagcgcatcg
    15541 gtcgccagca cgcctggccc gccggcattg gtgaggatcg aaagtctttt ccctttcgga
    15601 agcggctgtt tcgccagcac gtctgacata tcaaacagct cattaatcga attcacacga
    15661 agcaccccaa cgcgccggaa tgcggcatcc agaacgtcgt cgcttccggt catggatccg
    15721 gtatgggagg ccgccgcctg cgcggcctgt gccgtgcggc cggccttgat gacgataatc
    15781 ggcttgttct gcgccacttc gcgcgcagca gagagaaacg cccgtgcatt gccgatcgat
    15841 tccatataga tcacaatgct ctgcgtgcgg ggatcatcgc cgaagtaatc gatcagatcg
    15901 ccccagttta cgtccagcat gcttccggtc gagacaaagg aactgaatcc aacctgtttt
    15961 tgccgggccc agtccagaat ggctgtgcag agtgcgccgc tctggctcaa aaaggccaca
    16021 ttgcccgggg cggccatggt ggatccgaac gtcacgttca ggcggatgag gggattcatg
    16081 actcccagac agtttggccc gatgatccgc attccgccgc tgcgggcatt ctccagaaca
    16141 gcctgctcca gtttgattcc cggagctcca agctccttga acccggcgga gataatcact
    16201 gcgcccttga ctccggcatc ggcgcattct ttcaccaggc cgggaatcag ctttgcggga
    16261 gttacgaaaa cggcaagatc caccttctcg ggtatggcgg caatgcttgg ataggccttg
    16321 atccccatga cgctctggcg ggtcggattg acgggataaa ccacgccgcc gaacggattg
    16381 ctgatgaggt tccacaacag ggtgcggccc acgctgttgg gtttttccgt ggctccgatc
    16441 agggccacag atttgggctg gaagatggcg tcaagagggt gccccgtata cttcagtaca
    16501 tcccccacag gatccgtaat caggttgctg ccagaattca tagatctttt cctttcagta
    16561 aacaaatggc tgatttgttc gtggacactc taccagccgc atatcggtct gccaagcctg
    16621 tcgagcagtt aattacatgg ttttgtctgc gaggagttcc tgctattgtg cttgatgcgc
    16681 tgacagtaat tttgggactg tgtggagctg atgaagtcgc gatggttttt tctgacagcg
    16741 gggtctggtg tcttctggct gctggtttgc gcggtaagcg tggaaacggc ggcacgcagc
    16801 tgtctgtctc cgcggttttt cagtcatgaa atggacgagc ttctgtggaa gctggagcat
    16861 gagtcctgcg acaagccggt ggtgatgctg ggggacagcg tggggcgtca gatatcgctg
    16921 gctatgcggg gcagggggga cacgtacttc gcgcctttag ccaccagcgc ggccgtggag
    16981 acagcggggc agtattttgt ttttctgcgc taccttgagc ataaccggaa acccgaccgc
    17041 gtgattctct tcatgcgtaa tccgctggac ggcgatctta ggaaggtttg cacagaaaac
    17101 tacgtccagc gttgtttcac gcgctggcgc gagataggca gtctgacctg gcaggcacgg
    17161 gatcccgctt ttggcctgcg catgctttct tatcgcctcg cttcagcaaa ataccgcctg
    17221 catctgcagc gcactattcc ctttcttccc gtgaaagatt cttttgaggg cattcctgaa
    17281 gaccagcttc tggagcgcaa gaagagttta agaaagcagt cgctgaagca gcggctgatc
    17341 agtctgctcc tgccgcgaag taccgaaacc atatccgaat actacttcct gaaactgctg
    17401 gatgaatgcc gcagaagaag cattcccctc tacttccagc caacccccat gagggagagt
    17461 aaaatcagca cgccggagga gacgctgcga agaaaggcgc ttttcagccg aatgggagaa
    17521 ctggccccgg attatcccct gttttcatat tccgatgtcg tgaagtctta tcccgaccgc
    17581 tattttgtcg atggaattca catccagcag tcaaagctgt cccaggtcag cgaggaatat
    17641 gcggatttga tggatcaaat acacgcccca tgatctcctt aacaaatatg cttgatgtcg
    17701 cgaagcggtt tgatgacaat ccccggtcaa tatcaggaaa ttcttaagcc atgctcttta
    17761 attcagccga atttattatt ttctttgtgg tgctgtacgg gctgtatgtg gtgctgcctc
    17821 atcgtcccca aaatctcctg ctgctggcgg gaagctatat cttttatgcg gcatgggact
    17881 ggcgctttct gtccctcatt ctgatatcca ccgtgattga ttactattgc gcgcatgcca
    17941 tcgatcggag cgatatgccg cgccggaaga aggcgttcct gaccgtcagc atggtttcca
    18001 accttggact tctgggcata ttcaaataca gcaacttctt tgtccacagc atgatctctt
    18061 tgctcggacg ctttggcctg gaggttcagc cccttacact gaacatcatt cttcccatgg
    18121 gcatctcttt ttacaccttc cagacgatga gctatacgat tgatgtgtac cgcgggcagt
    18181 tgaagcccgc agtgaacatt ctggactttg cactgtatgt aacgcttttc cctcagctgg
    18241 tggccggtcc aattgaacgc gcttccaacc ttttcccgca ggtcatccgc ccgcgcaaaa
    18301 tcagccgcta cggcataaca catggagcct atttgatctt ctggggctat ttccagaagg
    18361 tgttcatcgc cgacaacatt gcgccgcttg tcgagaagct ttttgaaggt tcggccccgt
    18421 ataacggagt gcatgtgctt ctgggccttt atgcgtttgc attgcagatc tactgcgatt
    18481 ttgccggata ttccaatatt gcccgaggtt tggctttctg catgggattc cgattcatgc
    18541 tgaatttcaa cctgccgtat tttgcaacca atccaagcga tttctgggcg cgctggcaca
    18601 tcagtctttc cacgtggctt cgcgactatc tttatatccc gctgggcggg aaccggaagg
    18661 ggacctggtt cacttatcgg aatctggcac tcacaatgat tctgggcgga ctctggcacg
    18721 gcgcggcctg gaatttcgtg ctgtggggga tttaccaggg gattctgctg atcgggcacc
    18781 gctggtaccg gccctttgtg gcgcccgccg tgccatggaa ggccgccgca ctccgggtgc
    18841 tgcacacggt ggcttttttc catctggtat gcctcggctg gctccttttc agggcggaat
    18901 ccctgcacca gatcggagcc atgctgtccg ccctgttcac tggctttgtc tggcccagcc
    18961 agttggtcct tgagcatctt gtcgcggcca tcctcttttt cgggggcctc atgatggtga
    19021 tcgaatggtt ccagttcagg tccagcgatc tgtatgtggt tctgaaatgg ccgctgccgg
    19081 tgcgcgtggc ggtttatctg cttatgtttt acggcctcgt gatctttggt gtgcaggatg
    19141 cccaatcgtt tatctatttt cagttctaga ctgctggctt ccggagtggc ctggacggcg
    19201 gctgcggttt tctgcatcga aagctggtgc tggaaaacac tgccctcgaa atatttcagc
    19261 catgaggtgg atatccttct ctatgatctg cagaccagga cctacgatgc cgatacaatt
    19321 gtcctggggg acagcgtcgg ccggcagctt tcgcggtatt tggccggaca gaagaagtgt
    19381 gatttcatcc cgctggccac aaatggcgct gtggaaatgg cgggccagta ctatatcctg
    19441 attcgttatc tggagcgcaa tcgcccgccc tccgctgtga ttctgctgaa gcatcatgtg
    19501 cttcacggcg atatgaagcg cgtgacaacg gaaaattatc tccagcgctg ttttacccgc
    19561 tggtatgaga tgttgtccat gacgtggcag tctcaaagcc cgtcattcgg gttgcggatg
    19621 ctgagctatc gccttgcggc ggccaaatac cgcctgcatc tgcagaaact gattcccgga
    19681 cttcctcctc ccgaaacagg cgggaatgcc cggcaggtgg aggagcgtct ggaggtcctt
    19741 gagaaaaccc cgccgctgca gcagctgatg aaacgattat tcccctcgga tctaaactcg
    19801 atttctgaat attactttat ccgccttttg agtctctgcc agcagcgcaa tatcaggatt
    19861 tattatatcc cgtgtccgat gacaaacagc acgtggacca gatacaaatg cggtgtggcg
    19921 tatgccaggc ttgttcggcg gctgcaggag ctgtctgaag acttccctgt attgacctat
    19981 acaaccgaaa tcagagacta tccaaatggc tggtttgagg acggcattca catatcggca
    20041 gagaaacttc ctgttgtagc ggacgattac agccggttga tgacggagct gatgcggcaa
    20101 tgagcgctct attccgtctt tattgcttga aattgctgtc ttcaggtctg ttttggtctg
    20161 cggttgtctg tctgggaata gaggccagat gcctgaaaac actcccctcg cgtttcttca
    20221 gtcatgaggt ggatactctc ctttataatt tgcagacgag aaattatgat gctgaaaccc
    20281 ttgttcttgg agacagcgtg gcccggcaga tagcccttta tctcgctgct gagggagagg
    20341 cggatttcct gcagctgaca acgaacggcg cccttcagat ggcggggcag tactatattc
    20401 tgcagcgcta tctggagagg aacagggccc ccgaaaatgt gatccttttg aagcatcggc
    20461 ctcttcatgg caatctgaac cgtccggcaa cggagaacta tgtgcagcgc tgttttacgc
    20521 gatggcatga aattgcggaa atgacccggt ataccggcag tccggccttt ggactgcgcg
    20581 cgctgagcta tcgaatggcc acggcaaaat accggctgca tcttcaggaa cttgttccgt
    20641 ggctgccggc gcatgcagtt gtggatgatc cggaaagact gaacgagccc gccgacgggg
    20701 aaaacccgat gcggctgccg gtgcgcatgc tttctcccgg acagagaatc tccgtttcgg
    20761 aacgctattt cctgaaaatg attgacctct gcagccgcaa taatatacgt ctcggctatc
    20821 tgccatgtcc gatgccggat gagcaatgga aggattatca gacaggcccg gcacacgcgc
    20881 gtcttgtggc ccgcctgggt gaaatctcgc aggcgtatcc cggcctgcga tatataacag
    20941 aaatcaaaac gtaccccgca gactggttta ttgacggggt gcatgtcagg aaggcgaggc
    21001 tgccccgtgt ggcggcggat tacgcccaaa tgatgaatga aatgatgcag ccatgaaaat
    21061 acaacaatat ctgcaacgcg agaaacagat tcacaaggca tgggcaaaac tgaacaaggc
    21121 cgcagatgct cttgagtcga cgcgcgcgct ggttctgctg gccggggccc tcagcgacct
    21181 gcggggcgac atgccgtccg ggggaattgc ggaaatggcc ggccaggccg ggctcaccgc
    21241 cgcggaacag gaacagatcg agggccttct gaccggggag aaagatggcg ccgataccgg
    21301 catgcacaac attggagccc tcattgctct gctctggaga attatccggg aggagcgcct
    21361 tcacctgatg atgaatgctc cgcgtggtgc gctcttcctg cgaaggatgc tgcaggtggc
    21421 cctgcttgcc gcgggcgttt ttgccgccgc gcttctgatt cgcgccggct gttatgcggt
    21481 gcggctggcg gcatataatg gatgcagggt gacctactat cgtggcacgg attttaatca
    21541 gaagatcggc gtcagcgttg agccggcttt gcgcgcgggt tacaaaagga acaggccctt
    21601 ctggtatgct ccggcttcaa agtggtccgc ccgctgggag gcggatctga aggtgcccga
    21661 cgatgatgtt tacagtttct tcacacagtc cgatgacggt ctgcggctgt atattgatga
    21721 tgaccttcta gtggacagct ggaaggatca ggaatggcgc accagcgggc agcatgctga
    21781 aataaggctc tcatcaggat ttcatcgcat cagggttgaa cactatttcc gctctggttc
    21841 agcggcgatt gtagtgaaat gggccggagg ctctgttcct aagaacacgg tcttggaata
    21901 cccgtatctt cagaagcccg gcggaagact tcatgattaa agcggaatta aacagagcag
    21961 ttctgatcgg tgcctgcatc ataggggttg ctttcatttc ggttctggcc gtgggattat
    22021 gcgcgcgcga accgatgatc ggcgacgaag tgattcacta ttacatgctc gtcaatcagg
    22081 ctgaacagct ccccgctccg accgtggagg cgcatatccc tctgggcacc ggcaaggtgt
    22141 acaaacgcta ttatcctcac gtgttcctct ggcattatat cggggcgctg ttttggaaaa
    22201 tggccggagg aaaattctgg ggtgttcagc tttaccagtc gctgttctgg ctccagtatc
    22261 tggtcgtcgg catgcttctt gcacagcagg ccgacccgga gaggaagtgg ggtgtccctg
    22321 tttataccgc gctgctggcc agcctcccga tcagtctcat attttccgtg gcgttttatc
    22381 aggatgtggc cgccacggcc cagatcatga cttcctttta tcttcttcgt cgtggccgcg
    22441 cgctggcggc ggcggtgttt ctgggcatag cgcttgcgct taaggtcacc gtattcatta
    22501 tgcttccggc attcattgtc tgcctgatca tgtttggccg gcacagcgtg tcgtggccga
    22561 ggcttctggt tcgcgcggcg gccgccctga tgatcattgc ggccttgtgc tggccgatgg
    22621 ccaaaggact gcattactgc ggatttgcct attatcccac aaccatgctt cagctctatg
    22681 caaagaaact gggacttgtg aaagacatgg ctgtcgtgcg gtccgtcaag ccgggcgata
    22741 cagaggagca tcgcgtgaat ctgccggtga ccggggctcg atctgttcag gaagtggtca
    22801 atcatccggg cgatctgagg ataaccagaa actggctgat ctatttcggc ggagtcatgt
    22861 gggtggtggc ggcgggaggg ctgcttgctg ttttgcgccc agggaccaaa ccgctgcgca
    22921 tggccggcgg atgggcgttg gcggtgggcg catggtacat tctgattacg gccctccata
    22981 tgtgcaattc tcccgatgcg cgcttttttc ttccgggcgt tgtgctggcg atgctcccgt
    23041 ttgcgcttat gttcagccgg tggcccatgg ccaggatttg ggtgccgctc atgctggctg
    23101 tgtcgctggc ccagagcggg gtcgtgctgg cgaagacaca tcagctgagg agaatatccc
    23161 ccgaactgca gcagaccgtg gaatttctaa aagacaatcc cccggttcct cacggcgtgt
    23221 tcatgtatcc cgaagggaac tatcgcctgt ttccggtccg gcatgactgg tatctgggtt
    23281 tccgcctcaa agaattctgg atggcggata atgatgtccg aatccagatg ctccgcagcg
    23341 cggatctggg agcgatcgtc atcaagaaac acctgattgg ccgtctggac gacaagatgt
    23401 cgaatctggg catttatccc gactttttcg ttcgggacat ctcggccgat tcgaggtttg
    23461 tccgggtgtt tgagaacagc ggattcactg tgtattctgt tcccgcagag tgaaaacagc
    23521 ggcacgagcc ttcataattc cgcattttgc gagccttttt tattcgtgca gaggcgatct
    23581 gtttgttatg taatgagccc atgaaggaaa ccattgttat tgttcccgcc tacaacgagg
    23641 aactgaacat caaaacactc ctggatgaag tcaggtcggc ggggccggac ctggatgtgg
    23701 ttattctgaa tgacgggtct ctggaccgga ctgcggaggc cgcgcgccgg agcggggcca
    23761 aagtgattga tctgccctgc aatctgggcg ttggcggcgc ggtccaggcc ggattcagtt
    23821 atgcctatga gaacggctat cagtatgccg ttcgcattga cggggatgga cagcatccgc
    23881 cgtctgaaat tccgcgactg ctggaggcga taaaatcgag cgaggctgat ttaatccttg
    23941 gttctcgttt tctgggcgag aagtcctacg gttcaacccg tctgcgctac tgcggcatct
    24001 atctgctggc ccagtttctt tcactgatct gccggcggaa ggtcaccgat cccacttcgg
    24061 gatttcaggc gctccgccgc cctctgatgt attttttcag ccaggcctat ccgatggatt
    24121 accccgaacc cgaagccctt gccctgatgc ggagacaggg ctatgacttc gccgaagtgc
    24181 ccgctgtttt ccgcgagcgg caggcgggag tgtcctccat tcagggctgg ggaacgctct
    24241 attatatgct gaaggtattt ctggcgctga ttgtggatcg cgcgcgttct gtcgatcccc
    24301 ggctttccag gcaaagagta atggaggcaa tgcagcatgg gcgcgatgga tattaatgca
    24361 tatctgaccg gatggcagac ggccattgtg ccgcgaattc tgattggcct gctgggcctg
    24421 ctgattgttc tgatccttgc ccgcaccata cgctcaaaaa gaatttcact cgtgccggcc
    24481 attctctggt ccctggcggc cgccgtgctg atcattttct cgctgttccc gcagcagctg
    24541 attcgaaccg tgatcagcac ggaatacgtg atgcgcatgc gtatcatgat ggggtttgtg
    24601 agtctgctgg tgcttttgat tactctggaa tccacccggc aggcgcgcct gcaggagcga
    24661 tatgcgctgt tatgggtggc cacggcgctg gttattctga tctgcgcctt cttccccagc
    24721 gtcgtgaatc tcctccgcct gctgatggga atgaactata cggcagcggt agtctccgtg
    24781 gctttcacgt tcctgacact ggtgcttttc catttttcaa tatccatgtc catttctcag
    24841 gcgaaacagg cgcgcatcgc gcagaaactg gctctgcttg agacgcgggt gcgtgaactg
    24901 gaagaaagac gctgaagcat cagtcctgcg actgcacttc gaaggtgacg gaatgaatca
    24961 gcgctccaag atccttcttg aatccacgga ttccgtcgcg cgccggattg taaggatgga
    25021 cagagtagat tcggtaattt ccggtttccc ctgacatcag gcggtcgggc ggccgggtca
    25081 aagatacaga aaccgtgcag atgctgttgc tgatgtccag aaaggcagct tcatcgctcc
    25141 atggcggcat gatgagcagg gtctgtgttt ttccatcctg ccggtttgct gcgcacgtca
    25201 gggtcagggt cacagcctct ccctgcaaag gcatcgggcc gatcaggccg ctcccttcac
    25261 ggctccaccg ggcgggcagg ttcaggcctg ctgtatcctt cagggggatg tctgtgcgcc
    25321 cccagggccc ccggaagccc agaactccgc ttcccatgac gcgcgtatag cggcgggtcc
    25381 attcggaatc attcagccgc cggcttttca gcagcgtcag tgtcatcggg tattcctttt
    25441 ccaccgccgg cagcgccctc ttgctgtgga tgtgcggcag atggaattga tatgtttgaa
    25501 cggggaccag aacagcgtcc tgttccagaa ccgcatgggt ttcataagcg ctcaggaaaa
    25561 gcacgcgtct gccatccgcc tgtgccatta aatgggggat ggcggcaccc gcgtcatgaa
    25621 cccgtccggg tccgatcccg agcacgcggc tgccgggaac cgcggccagc ggaatgctcc
    25681 atgggtgata atcaatgatg gcaagatcac tgccaatgac atcgcgcatg ctttgaacat
    25741 acgcccgact gcctttttca tttcggagca gccagggggc gggccatcga acggcattca
    25801 gcaggcccag aaccagaact gtgattacgc ctgcgcctgc agccagacgg aggggccggc
    25861 ctttcagtcg gccggctgtc cagtcggcga tgactcctcc tccgcatacg gccggcggaa
    25921 gaaggagcat aagggttccc agaagtcttc gctgactcca caaacccaca agttcggctc
    25981 ctttcagata gagattcacc ggcaggacga gcagggccag aagcaggatc agcaggccgg
    26041 catgctgcct tctgtggagc agaaccgcaa gcagcaggat cccaagcagc aattcaagat
    26101 gcagccccat ggccgaatgt gcatccagca gcccctgggc caccacatgc ttctggtgcc
    26161 agaaaacgag cgagcagaac agcgggatgg cggctgcgca aacagcgccc agccggggta
    26221 aaacgcctga ttccagcacg gttgaaagca gcattaacgt ccggcgacgg aacaggagaa
    26281 gcagggaacc cgcggccaga atggccgcca gatacaccat cagaagccag tgtgtggctc
    26341 cgaggcggaa gaaactcagg aggctgccca ctgcaagacc cccgtaaggc ttgcaaataa
    26401 actgggtttg ccagaacagg gggagcagtc ctgcggggaa aaagagcgcg gccgaagaaa
    26461 ttctccggtt cccgtctggc gggagcatca ggacggcgat cagcagcggc agagcgatga
    26521 caagaaaaga gacatgatag ctgatcatga gaccaagcgc aaagcacagc gcgcgccgcg
    26581 gaatggtatt tttatcaagc agcagattca ccagcgagag gcagagaagc gatccggcca
    26641 cggcttcgga ataccacccg cggaatatcc agacgggcag gggagtggaa aagaaaagca
    26701 cccatgcaag aagcatgccc ggccatcgcc cgcgcgcaat tccaaggcac atcagggtga
    26761 gaagcgtcaa aatcccaagc acaggaatca gcccgtctgc cgctgggccg gggataatca
    26821 gatcgagtcc gtttgcggtg aagggcagca gcggataata aaacggcctt gtcatgcagc
    26881 tggtcaggtc gtccagcgca taggattggt cgcgggtcag gcggccgtgt cccgtcatgt
    26941 tcgggcggaa aagaaaagcc gcgcggtgcg gctccggaac ctgcagaaga agctcatcgg
    27001 tttcaaacag cggccgtccc tcgtgcatgg cctgcgccat caggcggtag gcgctgtcat
    27061 caagtccggt gagcgcctcc tgatgcgggc ggagcagcag aatgcttccg ctgagcagca
    27121 gcagtgcggc cagaatgcct ccaccgcctt tcagtttcgc gtaaaacatc cgaatcacag
    27181 gccgcagaag gaagaacagc gccccggcaa gcagtccgag agagaggagg gaatatatgg
    27241 tgaccgccat catacggcat gtgtctccgc tgcccgccgc agagcggcga gggttttatc
    27301 ggatgtggcc tgccacgaga aactgcccgc atgggcgcga cccctgcggg cgacatcctc
    27361 tcgctcggca tggggctgat tcagcgcatt caggatgact ttggccagtt cagatgtatc
    27421 tgccgggtca taatacattg ctgcctgtcc cccggcttcg ggcagggaac ccttgcgcgt
    27481 tgtaatcacg ggggtcccgg cggacatggc ctcgagaacc ggcagtccaa aaccttcgta
    27541 aagcgagggg aatacaaaaa cttccgcggc ggcatagagg ctgtccagtg catcgctgga
    27601 aactcctgac aggcgcagga ccctgccggg attcttcagc ctgtttattg cttgtcggag
    27661 acgctcttcg cccagtctcg cccgcccgac gatcaccaga ttatggggaa tgcggtcctc
    27721 cagcatgaca aatgcgtcca ccagctgatg cagattttta tgcgggtagg agttggccac
    27781 ggaaagaatg tacggtttat cccccgcggg attcggcccg tcggccgggt gatgcggatg
    27841 cgacgccaga tgcgtcacga ttatgcgctc gggcctcgcc gcggtgtatt tcagaatctc
    27901 ctgttttgag aaattggaaa tggtcaggat tccttcgcag cgccgtacgg ccagccgcac
    27961 aagaacatcg gtcgcaatgc gcgccggaag agtcaggtct tccgggaagg ttttatactg
    28021 catgtcataa atcgtcacga cctgagggac cggggaaaac aggcaggcgg tgtagccggg
    28081 agaccagagc acatccgctc cggaaatatt taccgcacga ggcagaagaa tctgctcctt
    28141 gagaatgcgt tcggatcgcc gggaagccgt acatggaatc cgtttgaatg cgaactggct
    28201 gaacatctga taacggcctt tcaggcaggc gtcgttttca ttgctggtaa aaagtgtgat
    28261 gcgctcggag gggtatccgg ctgcgatgcc gtcaagaatt ctgcacagat aggtttcaga
    28321 acctccgacc tccccgggga tgagaaacag ggtgttcacg ccgatgtgca tgagtcagtt
    28381 ccccgccttt cgggctttcc ccataagcgt ataagctccc acgatgcccc agcggacaaa
    28441 cagatgcgcc agcgcctgcg gggaaacccg cccggcgtcc ttcacggcgg catcgaattc
    28501 ctccttgaac tgcatgtgga aatgctggcc gctgatggat tgctcgtgcc agcggaaggc
    28561 ggccagggca gggggcgcaa caacagccgc cccgccgtgt ttccagaggc ggagcataaa
    28621 ttcataatcc catgctgctt tcagggattc actgagcgga cccgcttttt caaaggcgct
    28681 gcgccggaag aacatggccg gctgggaaac atagttgatg cactgaatca taaaacggca
    28741 tgagaatggg aagaacatgt gcttgaaacc ggttatcccg cggcggattt ccctgtcgtg
    28801 ctcatcaatg atggtgcacc gtccgaatac cagagcggct ttcggatgtt tggcgaatgc
    28861 cgcggtcacc cgctccagag ttccggggga ataaacatca tcggcgttca gccagcagag
    28921 gatgtctccg gttgcgcggc gcagcccttt gttaatggcg gaagcaggtc ccgcatcctg
    28981 ctcggacacc acttcatcca ggtccccggc atatttgcgc aggatatcct gcgttccgtc
    29041 tgtgcttgcg ccatccatca caaggtactc cagttcaccc catgctccgc gctggcggat
    29101 gacgctttgg atcgtgcgct cgatgaacgc ctctgagttc cggcatgccg ttatgacgct
    29161 gattttcatt tatgccgatt atgataaggt taaacttgaa caatacgatg caaaagattt
    29221 tagtagtccc gtggcagtca ttgcgactca aaatttgatg aattgacagg tggtattatg
    29281 tccttttggg aaaaaaagaa tgtgctggtg acaggcggag ccggttttct gggttcgcat
    29341 gtggtggacg aactgaaacg aatgggctgc aaaaacatat tcattccacg cagcgcacaa
    29401 tacgatctca gggagaaggg ggatatccag cgctgtctga aggacagcaa tcccgatctg
    29461 gtgattcatc tggccgccgt ggtgggcggc attggtgcga accgggagca tcccggaagc
    29521 tttctgtacg acaacgcgat catgggcatt tccctgatag aggagtgccg gaaattcggc
    29581 gtggcaaaaa tgaccctgct ggggacgatt tgcgcctatc cgaagatcac tccgattcct
    29641 ttccgcgaag agaatctgtg gaacggctat ccggaggaga ccaatgcgcc ctacgggctg
    29701 gccaagaagc tgatgctggt gcagtcgcag gcctacc
//
LOCUS       DAOQWR010000013        29631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1840_length_29631_cov_5.739113, whole genome shotgun sequence.
ACCESSION   DAOQWR010000013 DAOQWR010000000
VERSION     DAOQWR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29631
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_29631_cov_5.739113"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKK36_02115"
     CDS             <1..331
                     /locus_tag="PKK36_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93383.1"
                     /translation="RRIAGMALEDELDAVEAELADRFGPLPPPFVRLLKTARIRVRAT
                     ACAIDRVETREDKIMLMRGGDYLQRSGRFPRMNSDDATIRLDEILDELCRWRVSPENS
                     LKMPKKR"
     gene            784..1626
                     /locus_tag="PKK36_02120"
     CDS             784..1626
                     /locus_tag="PKK36_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR93384.1"
                     /translation="MIKSILVCTDGSAYGDTACEYAVDFASRLKARLMGLHILDSRML
                     EGPLMADISGWVGAQPFGTQLQQFRELMEKKGESIIEAFNARCEKAGIQTESWMKMGH
                     PARIILEEEARAELLVMGQKGEHADWIGDMMGSNVERIVRRSVQPCLITPMEFRPVTK
                     MLAAYDGSAHSSKALREALELSNALSLPLTVLTVRDGLSGEKATAAAADAQRLAKDHG
                     CEIESMVEEGESEDVILGTARSKECDLIVVGAYGHTRIREMILGSTTAHLVAYSEVPV
                     LLVR"
     gene            1655..2734
                     /locus_tag="PKK36_02125"
     CDS             1655..2734
                     /locus_tag="PKK36_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNR93385.1"
                     /translation="MPVEILYLWTAILTILVAVMSVLLLKRASRRSESVLLQNQINAL
                     SQQTSERMDQLRGAVEKTSAEVARALSGTQQAMGARLDTAAEVIRGVSTRLAALDESN
                     KRIFDVGRSISELEKVLRAPKLRGNLGELFLNELLAQILPPNYCQMQYSFSGGDTVDA
                     VIRLENGLIPVDAKFPLENFRKYSDSENDQQRSEFRKTFVRDVKKHIDAIASKYIRTD
                     EGTLDFALMYIPAESVYYEAIIKEEAGSQASLFEYALQKRCIPVSPNSFYAYLQTIML
                     GLKGLRVEESAREIMDSLARLRKEMERFEEDFQLVGKHIENAAKKFQDAGRRFITVNG
                     KLEQMEGISGTAPAALPEETLSARP"
     gene            2804..3550
                     /gene="larB"
                     /locus_tag="PKK36_02130"
     CDS             2804..3550
                     /gene="larB"
                     /locus_tag="PKK36_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNR93386.1"
                     /translation="MNLEQVLKKVAAGEIAVAEARRQIEGSLEQSLGFARIDMDRPRR
                     CGHPETIYCPGKTVEQIVEIAGTLHGAGQHVMATRATEDVYRAVAAAHPAARWHADAR
                     AVTLDAGPLPEPRGLIAVVTAGTADLPVAEEACLTAERMGARIDRIYDVGVAGLHRLG
                     ARLERLRKANVVVVVAGMEGALPSVVGGLIDRPVIAVPTSVGYSLNLHGLSALLAMLN
                     SCVPGITVVNVDNGFGAGVAAAMINRAGAL"
     gene            3547..4734
                     /gene="larC"
                     /locus_tag="PKK36_02135"
     CDS             3547..4734
                     /gene="larC"
                     /locus_tag="PKK36_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNR93387.1"
                     /translation="MKILRFDSIGGASGDMILGALLGLGVDAAWVEKQLADFLPDPFH
                     IETKPHESCGLNGVQTRVHIGGHHAHEHHRHEHDHEHRSFRVIRDMISNSRLDPKVKE
                     LSIRVFQRLAEAEGAVHRKPAEDVHFHEVGATDSIVDIVGSCLACVKLGIDAVSVDRL
                     PLSSGSVTCQHGVYPLPAPATLELLKGFSVEQTEESVEMVTPTGAALLMSWRSEQAPA
                     RGRILQSVNSFGHHHLHHRPNVLRATMMESEAPAASGPECLVMETNLDDMTPELIGTL
                     MDALLHAGALDVFTTPVQMKKNRPGMLLTVLCEASRADVLKNIIFTESTTFGIRQYAV
                     ERTVLARRTVEVETEYGRIRVKIGTWKGSDVTRSPEMDDCAAAARAHNVPVRKVYEAA
                     SRL"
     gene            4731..6380
                     /locus_tag="PKK36_02140"
     CDS             4731..6380
                     /locus_tag="PKK36_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNR93388.1"
                     /translation="MNRLFHFMLTALLCAPGAQAAKDALSFPAGRYRDGDVLSVQEHD
                     VVLREQGWVSYALRFEWPEDYQFVILARRDGASKQVPSLKVSVDDEEVRLQPVISEAW
                     ATYSFRCAVNKGERNVKLSSPVSGADDIHLASVVVVAPRKAAGVTLLESSRSAEPLKD
                     ANLLFHEKLIARIRETRTGQLRVQVLDKAGEPVKGVSVRVSQQRHSFLFGASQLTAAY
                     DGTLSMRLRNEYKEQFLRRFNCVGTGEALTWRYMEPQPGRYYYTVGDKMIDWARARGL
                     AVWGEDVYSECAEELPAWASAVSDARMRSVAGLRARVMANRYRGRVDALAVNGNMISC
                     GHPGERFGAGMRKQLFHEIRASDPSAKLFLNEDIDLDTGRPETFMQPLRALLESGIPA
                     DGIGIRARFDRQPNAFRVERSLDALAQLGLPVRITGYACDDPDEQARLRAMESFFRVA
                     FSHPAVEGISVRDFWFSESAGFPLLNADFSPSPLGHLYERLVFSEWWTETEGLTDKNG
                     IFETRAFLGEYEVGVALSPTREIKQKAVVTSGDNPPVVFSY"
     gene            6477..7343
                     /locus_tag="PKK36_02145"
     CDS             6477..7343
                     /locus_tag="PKK36_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNR93389.1"
                     /translation="MTTREDTMQDYQRRIERVLRFIREHQSEPMRLKELAEVACFSPF
                     HFHRIFSAHAGETLSEYIRRLRLESSAERLCRTTDAVTEIALDAGYATHGAFTKAFTQ
                     HFGRNPSSFRKTGKMDFVPGRLLMMSKQKETEMKVQIREIQEQAALAVRRTGAYDKAA
                     EEAWKILCTYAGPRGLIGPKTKFIGISHDDPDQVAAENLRYDACITLSSDDKGSGEVK
                     RLTIGGGRYAVTVHAGPFEYLKKTYDGIYRDWLPSSGETLRDAACLEIYLNDPDTTPP
                     ADLRTEVCIPLE"
     gene            complement(7352..8203)
                     /locus_tag="PKK36_02150"
     CDS             complement(7352..8203)
                     /locus_tag="PKK36_02150"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNR93390.1"
                     /translation="MKKIGVTANIRKPHTPAVLERLAAAAGEMGAELVCCDDTAQSLP
                     SIRKISRHRMVAEIELFMALGGDGTVLRAISILNGCDVPILGVNLGSLGFMTTVTEDK
                     LEHALRCVQEGKCGISERTLAVCSLSGSKKEYIALNDVAIGWGRSSRVVTLNVRADGE
                     EVSSYVCDGIVVSTPTGSTGHSLSSGGPIIHPEVRAFVLSPICPHTLSNRPLVIPDTC
                     VIEVEVLAASKTLLLTMDGQEQLEVSKGDVIQIARSPHSAKLVRLPDYNYYSLLRHKL
                     HWRGSTV"
     gene            complement(8200..8934)
                     /locus_tag="PKK36_02155"
     CDS             complement(8200..8934)
                     /locus_tag="PKK36_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNR93391.1"
                     /translation="MSRMRLDQLLVERKLAESREKAQRLIRAGEVLVNGHPQTKPGHE
                     FDPEIEITVKASMPFVGRGGQKLEAAFSAFGIDVRGWVCLDIGASTGGFTDCLLQHGA
                     QRVYAIDVGAGQLHWQLRNDPRVIVMEKVNARYLKPEDLAERADFAVVDVSFISLTKI
                     LPAVTALLQPCAELVTLIKPQFEAGREKVQRGGVVRDEAVRAEVVAAVKQFGTGTLDL
                     EWMGVCASPIRGPAGNVEFLAWWKKK"
     gene            complement(8931..9722)
                     /locus_tag="PKK36_02160"
     CDS             complement(8931..9722)
                     /locus_tag="PKK36_02160"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNR93392.1"
                     /translation="MSRRQKFIEVLDSTETWLLQEKAEGRKRVEVSNETRKSLGTAPF
                     AAPSSRRPEPPPDRRPAAASAPQVLDPGLAAIEKEIAACTKCPLAPTRTNTVAGQGHP
                     RPEIVFIGEGPGADEDKQGRAFVGRAGQLLTSIIGAMGLTRDEVWIGNIVKCRPPGNR
                     TPFPDEMEACMPFLKRQLAILKPKVIVCLGATAVKGLLGEQTGITKLRGHWRSFEGID
                     VMPTFHPAYLLRNASGKAPVWEDMKAVLMHLGKPVPDHRKKSQGK"
     gene            complement(9779..10687)
                     /locus_tag="PKK36_02165"
     CDS             complement(9779..10687)
                     /locus_tag="PKK36_02165"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR93393.1"
                     /translation="MSRLTASPSDAGMRLDAWLARTLPGCSRSRWQQLIRDGHVKIGG
                     SPCKPQHALAAGEEIEYEIPAAKPVTVEAEDIALDVLFEDRDIIVINKPAGLVVHPAP
                     GNYTGTLVNALLHHCRDLEGIGGELRPGIVHRLDKDTSGVMVAAKNERAMQFLVNAFK
                     NRQVHKEYLALVAGRMERISGTISTLIGRSRGDRKKMSVRPASGGREAVSHYRVEEQF
                     AGAALLRITLETGRTHQIRVHVAHIGHPVIGDRKYGRAGNLLDARRQMLHAETLTLPH
                     PVTREPMKFHAPVPEDMQSLLNRLRG"
     gene            complement(10659..11453)
                     /gene="lgt"
                     /locus_tag="PKK36_02170"
     CDS             complement(10659..11453)
                     /gene="lgt"
                     /locus_tag="PKK36_02170"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNR93394.1"
                     /translation="MHQICFHIGSRPIYWYGVMVALGFLLATAHWSWLARRRGLPPGF
                     GSNLAFFIMLSGILGARVAYVLANLSYFAQHPLHVFRVDEGGLIFYGGFFGALAAVIV
                     MARVQKIPVWTLGDLAITGVPLGHAAGRIGCFLNGCCFGLPTDCPLGVRFPAGSPPDL
                     MHPGTTLHPVQLYETAGNLALYGLLLWFYLRGRRSGATVALYFILYPLLRFSMEFFRG
                     DERLHGMGLTFAQLVSAILLVAGLIIMILRYDKPEPSLEQTHSKPV"
     gene            11530..13917
                     /locus_tag="PKK36_02175"
     CDS             11530..13917
                     /locus_tag="PKK36_02175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR93395.1"
                     /translation="MALKADKVSSMLCSHCGAAMDVSSQDPFSTALCPACGRSQIVPM
                     QFGSILLLEPLGAGGMGAIYRALDPMLDRYLAIKVMQTAGAADDQFVENFFREARAAA
                     ALNHPHIVQIYSCGREYGQPYIAMELLEGGTLDEMIHSSGTVDELDVLQIGISVSEGL
                     RAAGEIGLTHGDIKPANILFDSHHKAKIADFGLARFMQQGSSAEIWGTPFYIAPEKLR
                     KQKEDQRSDIYSLGATLFHALTGQPPFDGKTATDVAVARLDQPPPDVRDRNPGISPEV
                     AAVIARMLKENPHERYPTYASLIADMKSVLAVLRQKRGSRPMRPKMRKSHAGAFVAAA
                     LFALIAAGIWMSQREGSGPPEGPLSGPSRPEEPAVPEMRGSKPFEGVRGTAMERILNL
                     FAAGNVMAASESLQALYEKIPPDSPDRSWICLFQALMLWYDGNGGSGDELLDRALAGF
                     AGSDASAGALIAAYMKGNMDEAAVEASAGEQPQWVGDFTGFVAGMNHARQGRKDEADV
                     RWRTYLQTTNDKPAWVYVLKPLAQKFTESHARWERLNAEVTALLEARKPGEARVLLRD
                     FKLTAGPVFAAETDQLFEKVQALEASLTEAEAARLAEQQAAAAARKKAKAESEVAKVR
                     KAVESGRRHYPSRDFDRALAAIEKLRPAMTTEEGWVEYQLAREKVSRLDAMHRYLQNA
                     LRTRPCRISVFGKNTRVAGATSRGVRVAIGTQADMVREWQEITAAQYVGLIRQYLDAE
                     QKTVREKADDHLSLAVFASETDGGQLAAQLARKAVALDPAVRDLGLRLLPDLDWE"
     gene            complement(13949..14025)
                     /locus_tag="PKK36_02180"
     tRNA            complement(13949..14025)
                     /locus_tag="PKK36_02180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13989..13991),aa:Asp,seq:gtc)
     gene            14134..15609
                     /locus_tag="PKK36_02185"
     CDS             14134..15609
                     /locus_tag="PKK36_02185"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNR93396.1"
                     /translation="MKVPSRILEKLKKLPDSPGVYIMRDRTRRVIYVGKAVSLRSRVR
                     SYFQPGTYRKAPVKVRGMINSVADFDFLELRTEEEAALTEGRLIKDYKPRYNILLKDD
                     KRFPLIRVDLRERFPRFERCRLHKEDGAHYFGPYANSGAARAAREFVERRFGLRNCPP
                     ASPDAETYRHCHNDVIRYCSAPCIGKVSRDEYLQRVEEACAFLRGERVEYLHEVRAAM
                     QAASEKLDFEKASALRDTLFLLQEAVKRRSLARKTPLMKQESARAGVLELQAALNLPR
                     PPAVIEAYDISNISGTFAVASMVSSVNGIPQPGRYRLYRIRTVQGSDDPAMMAEVVKR
                     RYSRLQREGRDMPDLVLVDGGITQVRAAARSLRELGLSDIPVAGLAKRFEELVQDRAG
                     GAARVLLPLDSCALTMVQQLRDEAHRFALTFHRRIRGRRIRESILDDIPGIGPARKAL
                     LLRHFGSVRRMQKATVEEMARVPGLGRAMAELVCGRLAKDG"
     gene            15667..17556
                     /locus_tag="PKK36_02190"
     CDS             15667..17556
                     /locus_tag="PKK36_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNR93397.1"
                     /translation="MSENGVIEIQTDRVERLACSQCDARMDVSKIPAFTIAKCPQCGA
                     PMPVPARLSQFLLFRELGKGAMGAVYQAFDTTLARHVAIKVMSSKLGANRAFVERFFK
                     EARALAALNHPNVVQIYSCGEEKGQPYIVMELVPGGRVDALMEESRISELRGLEIGVD
                     VASGLKAASDIGLVHGDVKPENILFNRDGLCKVVDFGIARFAGEQSDGGGIWGTPFYI
                     APEAVRGQAVDQQADIYSLGATLFHILAGRPPFDAETAKEVILARLKSPAPSLTEFCP
                     EIHPETARVVGRMLEESSVRRYPTYESLLADMRSVLERVRAESRVRPGSRKKKKQAGH
                     PHAVTAVIVTVLALGILGVIFAGMKNARKSVSRQPAVPQVPPGTVLVKPSSAASPSPQ
                     APAASAPAARPSSPAAASPPQAGAFELRLTTKDAGGADAYVQGGKSGGDKNFGQEDVL
                     WLKSNDQVPIHLVRKVYLRFGLDDLAERKVRDAELKLTATPGGSNRNDLYKIKLWGLA
                     KPSSKGDWAEIDDGSFDAWANRLTWNNAPGNDFRSPDGMSEDAVLLCVVNAHPNPPVG
                     AAVSFSNADSVSKDALVDFISARRGSVTFMLTAEGGPSNVYGWKFASKEHAYLNPPEL
                     TVRGE"
     gene            complement(17690..18142)
                     /locus_tag="PKK36_02195"
     CDS             complement(17690..18142)
                     /locus_tag="PKK36_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93398.1"
                     /translation="MTDFQAHIPIPRQLWLRDRCIAVETQFQKSIGRGTFVTRFLPLL
                     VILALAYWNRIQTPPRVAGWIAVPVTAAAFYFALLVLSHRFHDIGQSGANLLQIVLPV
                     FVWLWVGGDLMAKLPPRLWIGTAAVLAVWPVIVILRLCLQRGTQTQAA"
     gene            complement(18153..19940)
                     /locus_tag="PKK36_02200"
     CDS             complement(18153..19940)
                     /locus_tag="PKK36_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR93399.1"
                     /translation="MTIAAEAKREWNEQHQHPLGFKTRRGLNWFTVGLMYAMFYMCRY
                     NFRFAGAGMRAEFGWSTGDIANIAAWFSIAYGTGQLVNGLFCDRIGGRASMLIGAIGS
                     ILVNVLIGFSPWTSTFMAFAAMWMVNGYIQAAGTPGMVKINAAWFNKEERGLFSGIFG
                     FMIQSGQAMISMLAPWILAGFSFLALVVGPGEWRMLFTIPPIFLGLVAIWFYFISAQS
                     PDDAGFHGTIQDDVDNSAGTTVPVLESFKFVFSNPLVWFYAVAYGCTGAVRHAADQLS
                     PLYFEDVLGFNMKTNLPLIASFTLVLVPIVAVCGSVISGWASDRFFRGNRYPVAMVLY
                     YGEACVIAAATTVMALGIIGPTTAGILLGCLFLVLIAVTANSTHSIVGAAAPVDIGGK
                     KMAGFASGVIDSFQYYGAALGLFITGLLLDVSLPGNYTVWFGIMTCFAFFGGCAMLSL
                     LLRQKKKAAASRAVIGFMVLLVISGAAFGISKYRTMKPVAIAHYADAAQSAFQAGDTR
                     DATDQAQRVLRLAPGHEQAMQIVDNVHDQFVAAAETALAAGDVQAAIENAQNAINLKG
                     RETPALKVLAEAKALDKAQQKAEKEAIEE"
     gene            20099..20536
                     /gene="tsaE"
                     /locus_tag="PKK36_02205"
     CDS             20099..20536
                     /gene="tsaE"
                     /locus_tag="PKK36_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNR93400.1"
                     /translation="MKKHYTTKSPEETCSVAAALAAELQPGAVLAFHGELGSGKTCFI
                     QGLAAALGVEAAVSSPTYTLVNEYRGRLPLHHMDLYRVADAGEALHLGLDEYLYGDGV
                     TAIEWPEVVAGLLPSSALHIYLRMGIGLDDREIFFRTGEEPPP"
     gene            20533..21219
                     /gene="tsaB"
                     /locus_tag="PKK36_02210"
     CDS             20533..21219
                     /gene="tsaB"
                     /locus_tag="PKK36_02210"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNR93401.1"
                     /translation="MKILAVELSAFQGSAALSGPDDFSLQEVWDQERGRHEGVFSRLS
                     ALLSQAGWGWGDVDCFAAGRGPGIYSGLRTALTCAQTLALPGEKSVYCVSSGAVLARG
                     ASREMNASRVAVVGDARRSLLWYGVFDFSLSETDSPAEWQMVKSSELCARLPAGVPVV
                     SSDWERLEPNLAAQGLDGLNWIRGNRYPRARDLAALVRLRMEQGLPSEKPVPVYMHPP
                     VLIAPRFPPA"
     gene            complement(21270..22946)
                     /locus_tag="PKK36_02215"
     CDS             complement(21270..22946)
                     /locus_tag="PKK36_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNR93402.1"
                     /translation="MIRKDKIVVTVDNGSKLEIKPGTCVRDLLKKKENEAGLPYLGAL
                     VNNDMVSLSFPIEVDSKIQMLTLANSHGWRIYRNSAAFLLAKATYDIFPKALLSIEHS
                     LGDGYFCNFEMNGTKGISKKQIKDIEQKMHQIVEADLPIVRRKIEYSDAIGHFEGQNR
                     HDTVNLLRYSNPPKVVVNTCEEYMDLFHGPLADRTSALQHFKLFPYEDGFVIQFPDRD
                     NQTPKMSPFERQPYLFDIFKSYKAWGRIVNVRTVGDLNTIIASGKLRDFIHISEAYQE
                     KKIAEIADTITAQGGKIKWICIAGPSSSGKTTFAKRLCVQLRVNGLRPVAISVDDYFV
                     DRTRTPMDENGEPDFENIETVDLKLFNEHLSLLDRGETVELPTFNFGAGAREFRGKTM
                     KLESDQICVVEGIHSLNPRLTEAISPDHKYKIYISALTQLNLDYSNRVSTTDNRLIRR
                     MIRDHNYRGNPALMTLKMWPSVRRGEKKWIFPYQAEANTAINSALDYELAVLKPYVEP
                     LLAEVKPYDPQYADARRLLEFLSRFNAASSSLVPPTSLIREFIGESGFEY"
     gene            complement(23050..24831)
                     /locus_tag="PKK36_02220"
     CDS             complement(23050..24831)
                     /locus_tag="PKK36_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNR93403.1"
                     /translation="MKTNPTCLTLLLLCLAAMPGFCSDSRNKDYGFIEVTGSDVRLPV
                     ADASRMFGPGNSVPLLQENPEFYIVALPDGITAAVGKLDRKGNPIAWVTQQKQLIFGD
                     RARAVSGFLRLRPGDEFRILQETPDARIIEYTFFQNRAELSIPKALPAGLAYNPPEPE
                     PAAEEPQPEPVRAVEAKLPEPPVVRPVPRPRPVPAPVSVARARPADSTPIEVPVKEVK
                     TPAAPPQPVLVAPEPEAPVIPEPAPAAPPTVQPAPAPAAATEPPEIAAREAAPSADPD
                     LPAATPEIPSGISEPEPLLAEQPAPPEESEAAGPAAETEVSADVATAAGEIETTESAP
                     AEPPPAAAPAGTVVRFLDKYMISSYILLVIVIIEGLFIVRLRRQRRTVPAAAGGEDAV
                     VLLPQLDTSRFRKYFQSLRTSTGQNSGPFGEFSTYDLVYYLHTEKETGGLELEQRKGM
                     VSAEMFFSNGEIVHAFVGNKVDDEAVLEILCMPCEVFTYTHLPVIIEQHTIHNPTVNF
                     LIAAAPAAAAPADTSVEAPASESGDEGETVQEPAEDAAKTLLEGLLEEKDGKTDSTPG
                     DGEETDPEWDEIMKSLKVLDDLNDKPE"
     gene            24977..27322
                     /locus_tag="PKK36_02225"
     CDS             24977..27322
                     /locus_tag="PKK36_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNR93404.1"
                     /translation="MSNSVETESVVWRGAWGFIFLGLGIILMLSLISWRPGDLSLIQD
                     PPNEQGTNLIGPAGAWMAFAGFGLLGVTGYLLPVVLWVQALFLFLNKSGSFRAKALYQ
                     LVVLLSLACIVELQPDLWEGLRTHLNIFYAGGLFGHLLATAFLVRLLGVAGCAIVAVC
                     ALLTALVLLFEIEPVQTLGGVGVALAAVPRKIGSMWRGARDRRAAIEEEERELERQRR
                     QLEKVVRRQERRRPAPVVESEPEPEPVQPPSPPREPRPPRREKPRPAPVEPPGPSAAD
                     SLPSPTPQYTLPAMTLLDRPPPARDRQIKENFSESARILQETLADFGIEAEVTNVERG
                     PVVTRFEVLPAAGVRVERISALSNNIALAMKAVSIRVQAPIPGKGVVGIEVPNSSTTL
                     VYLRELLEGEAWQKSRAGLPLAMGKDVGGNDIVADLSDMPHMLIAGATGSGKTVCMNS
                     LLAGLLMSRTPDQLRLMLVDPKIVEFSFYNELPHLVVPVITDPKKVGLGLRWAIMEME
                     KRYKLFAKAGVRNIASYNSRPKVTQEELFEEAAEPADNASKMPDRLPYIVIVVDELAD
                     LMMVAQAEVENYIARLAQLSRATGIHMILATQRPSVNVITGSIKANIPARIAFQVAQR
                     NDSRTILDANGADKLLGKGDMLFLSPKTGKLVRAQGTMTSDSEIRRIVDCWKEQGRPQ
                     YETAIKEQIENKKTELPEMNDDDELLDPAIEIIRQTRRASTSSLQRRLRIGYTRAARL
                     MDVLEEKGIIGPPNGSDPREILIDLDGEIPQNPDEDEEMDD"
     gene            27322..27885
                     /locus_tag="PKK36_02230"
     CDS             27322..27885
                     /locus_tag="PKK36_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR93405.1"
                     /translation="MPRIGQIFRESREKKGVTASQAAQATRMKVQTVQHLENNDFGKI
                     AAPAYVKGFIRLYAEYLGLDPAPLIREYLNGLGETRSPIAPPVKTSPWRDVLKKAAGR
                     LSASLKKISPAVWIKLAGAAVLLLLAWSLLLGISAAVRRKAAGEPPSEEFTARPEAAS
                     GFLEDIPEPYWDVLDPSSRPVQDGAAL"
     gene            27882..28499
                     /gene="pgsA"
                     /locus_tag="PKK36_02235"
     CDS             27882..28499
                     /gene="pgsA"
                     /locus_tag="PKK36_02235"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNR93406.1"
                     /translation="MKWNIPNILTVSRLFLALILLMLIGMPYPLTKTLALLVFVAAGI
                     TDFLDGHLARNVYGVTAFGRLMDPLTDKVLVCSALVSFVGIRLPGWDRPLLPVWIVVV
                     VISREFLVTGLRLLASSMGHIIAAGRWGKHKTVWQIIAISVILLGLAVRYDVLGHLSP
                     AALEKYDFAFGLIAYAVGLAVTMITVASGAMYFAEHTELLRHKIQ"
     gene            28522..29256
                     /locus_tag="PKK36_02240"
     CDS             28522..29256
                     /locus_tag="PKK36_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR93407.1"
                     /translation="MKKCFALFAVTAATAFAQAPEAVAQRMSLGEIIETGGWLMYVLG
                     AMSVAGLAMIIYFFMVLRPERVAPRSFLKELSTELKQRRFDRARELCRCEDHAAACVA
                     LSGLNHLGSSRETEPMLLKEIMEGEGSRQASMLQGQIQYLMDLGVLSPMVGLLGTVMG
                     MLRAFNAVALDIAKAKPMILAAGVSQALITTAAGLLVGIPAMIFYSYFRGRSSRLIAS
                     LEAVSADLLGELLEAENLPSDQSGSK"
     gene            29253..>29631
                     /locus_tag="PKK36_02245"
     CDS             29253..>29631
                     /locus_tag="PKK36_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR93408.1"
                     /translation="MNFRRQNRPEALSFQIAPMIDVVFLLLCFFITSQVFSQWETEID
                     IALPTAKTGEVPQRLPGELIINILADGRTVISGQEQPEDRLGPLLKRLSGFFPGQPII
                     IRADQKTPYQHVIRVLDLCRQRSS"
BASE COUNT         5755 a         8602 c         9275 g         5999 t
ORIGIN      
        1 tcgccgcatc gccggcatgg ccctggagga tgaactcgat gccgtcgaag cggaactggc
       61 ggaccggttc gggccgctgc cgccgccgtt tgtgcgcctg ttgaagaccg cccgcatccg
      121 ggtccgcgcc accgcctgcg ccattgaccg cgtcgaaacc cgcgaagata aaatcatgct
      181 catgcgcggc ggagactatc tgcagcggtc cggccggttt ccgagaatga actctgacga
      241 cgcgacgatc cgcctggatg aaatactcga tgaattgtgc cgctggcggg tgagtcccga
      301 aaacagcctc aaaatgccaa aaaaacgctg aaaaaggtcc aaaaacggca aaaaatcgct
      361 caaaaacggc aaaaaacggc ccaaaaaccc aaaaaaatgc aaaaacagag aaaaatgaag
      421 caaaaatatc ccgtcagcgt ttgcgattcc aaaacgggcc gaaactgtcc tgggcctggg
      481 gcaccgcggt cggcggccga tggatgctga ctgggggcac ataatttccc gctgttcagc
      541 ccgggatttc ccgcgccggc ttgaaatagt actaaaatag tactatttcc gtgaaaaacg
      601 cgattttcag ggcattttcc ccgtttttat cgaaaaaatt gccctttggg ggtcgttttc
      661 gctgtttttc ttcattttta cgcgtttctg cgtctcctgc gcggctggaa acgcgcgctg
      721 gacttttggc gcgccggggt tcaatatgat tacaaagagg cagacaacag aagggagacg
      781 gccatgatta aaagtattct ggtttgcacg gatggatcgg cctacggcga cacggcctgc
      841 gagtatgccg tggatttcgc ctcgcgtctg aaggcgaggc tcatgggcct gcatattctc
      901 gattcccgga tgctggaagg acccctgatg gccgatattt cgggctgggt gggcgcccag
      961 ccgttcggca cccagcttca gcagttccgc gaactgatgg agaagaaagg cgagtccatc
     1021 attgaggcgt tcaatgcccg gtgcgaaaag gccggcatcc agaccgagtc atggatgaaa
     1081 atgggccatc cggcccgcat tatcctcgaa gaggaggctc gcgcggaact gctcgtgatg
     1141 gggcagaagg gtgagcatgc cgactggatc ggagatatga tgggctccaa cgtggagcgc
     1201 atcgtccgcc ggtcggtgca gccgtgcctg atcactccga tggagttcag gccggtgaca
     1261 aaaatgctgg cggcctatga cggcagcgcg cattccagca aggcgctgcg cgaggcgctt
     1321 gaactgtcga acgcgctttc gctgcccctg accgtgctga cggtgcgcga cggactgagc
     1381 ggggaaaagg ccaccgccgc cgcggccgat gcgcagcgtc ttgcgaagga tcacggctgc
     1441 gaaatcgaaa gcatggttga agagggcgag tccgaggatg tgatcctggg caccgcccgc
     1501 tcaaaagagt gcgacctgat tgtggtggga gcctatggcc acacccggat ccgcgaaatg
     1561 attctcggca gcaccacggc tcatctggtg gcctattcgg aagtgccggt gctgctggtg
     1621 cggtaggctg ccgggcctta atttggacga cgttatgcct gtggaaatat tgtatctgtg
     1681 gacggcaatc ctgaccatcc ttgtggcggt catgtctgtc cttcttctga aacgggcctc
     1741 ccgccgcagc gagagcgtcc tgcttcagaa tcagatcaat gccctcagcc agcagacctc
     1801 ggagaggatg gatcagcttc ggggcgcggt cgagaaaacc agtgcggagg tggcgcgggc
     1861 cctgagcggc acgcagcagg cgatgggcgc gcggctggac accgccgccg aggtcattcg
     1921 cggcgtcagc acgcggctgg ccgcgctgga tgaatccaac aagcgcattt tcgacgtggg
     1981 cagaagcatc tccgagctgg agaaggtgct gcgggcgccc aagctgcgcg gcaacctcgg
     2041 cgagcttttc ctgaacgagc tgctggcgca gatccttccg ccgaactact gccagatgca
     2101 gtattcgttt tccggcggcg acaccgtgga tgcggtcatc cgcctggaaa acgggctgat
     2161 tcccgtggat gccaagttcc cgctggaaaa tttccgcaaa tattccgatt cggaaaatga
     2221 ccagcagcgc agtgagttcc gaaaaacctt tgttcgggat gtcaaaaagc acattgatgc
     2281 aatcgcctcg aagtatattc ggaccgatga gggcacgctg gattttgctc tgatgtacat
     2341 cccggcggaa agcgtttatt acgaggccat catcaaggag gaggccggat cgcaggcttc
     2401 cctgtttgaa tatgcccttc agaaacgctg catccccgtt tcgccgaaca gtttctatgc
     2461 ctatctccag accatcatgc tgggactcaa gggcctgcgg gtcgaagaaa gcgcgcggga
     2521 aatcatggac agtctcgccc gcctgcgcaa ggagatggag cgcttcgaag aggattttca
     2581 gctggtcggg aagcacatcg aaaatgccgc aaagaaattc caggacgccg gcaggcgctt
     2641 catcaccgtg aacggcaagc tggagcagat ggagggcatt tcgggaaccg ctcccgcggc
     2701 gcttcccgaa gaaacgcttt ccgcgcgccc ttaacgctca cggcttccgg cgcggctgca
     2761 ccaagccttg acctcggacc cgcttgagga cattctagga ggcatgaatc tggaacaggt
     2821 gctcaaaaaa gtggccgccg gcgaaatcgc cgtcgccgaa gcccgcaggc agattgaggg
     2881 gtcgcttgaa cagtcgctcg gctttgcccg gatagacatg gaccgccccc gccggtgcgg
     2941 gcacccggaa accatctact gtcccgggaa aaccgttgag cagattgtgg aaatcgccgg
     3001 aacgcttcac ggcgccggcc agcatgtgat ggccacgcgg gccacggaag atgtgtacag
     3061 ggccgttgcg gcggcgcacc cggctgcccg gtggcatgcg gacgcgcggg ccgtcacgct
     3121 ggatgccggg ccgcttcccg aaccccgggg actgattgcc gtggtcacgg ccggaacggc
     3181 tgacctgccc gtcgccgaag aggcctgcct gaccgccgag cgcatgggcg cgcgcattga
     3241 ccgcatttat gatgtcggcg tggccggcct tcaccgtctc ggcgcccggc ttgagcgtct
     3301 gcggaaggcg aatgttgtgg ttgtcgtggc cggaatggag ggagccctgc cgtcggtggt
     3361 gggcgggctg attgaccggc cggtgatcgc cgtgcccacg agtgtcggct acagcctgaa
     3421 cctgcacgga ctctccgcgc tgctggccat gctcaactcg tgcgtgccgg gcatcacggt
     3481 ggtgaatgtg gataacggtt tcggcgccgg tgttgcggcg gccatgatca accgcgcggg
     3541 agctttatga aaattctgcg ttttgacagc attggcggcg cgagcggcga catgattctt
     3601 ggagcgctgc tggggctcgg agtggatgcc gcatgggtgg agaagcagct ggcggatttt
     3661 ctgccggacc cgtttcatat tgaaacaaag ccgcacgaaa gctgcgggct caacggggtc
     3721 cagacgcgcg tgcacatcgg cggccatcat gcgcacgagc atcaccgcca tgagcacgat
     3781 cacgagcacc gttcatttcg tgtcatccgc gatatgatat caaacagccg cctcgacccg
     3841 aaggtgaagg agctttccat ccgggttttc cagcgcctgg ccgaggccga gggcgcggtg
     3901 caccggaagc cggcggaaga tgttcatttt catgaagtcg gggcgactga ttccattgtg
     3961 gatatcgtgg gctcctgtct cgcctgtgtg aaactcggga tcgatgcggt ctctgtggat
     4021 cgcctccccc tgagttcggg ctccgtgacc tgccagcacg gggtctatcc gctgcccgcg
     4081 cccgcaaccc ttgagctgct caagggtttt tccgtggagc agacggaaga gtccgttgag
     4141 atggtcacgc ccaccggcgc ggcgcttctc atgagctggc gaagcgaaca ggcgcctgcg
     4201 cggggacgca ttcttcagtc ggtcaacagt ttcgggcacc atcatctgca tcaccggccc
     4261 aacgtgctgc gcgccacgat gatggaatca gaggccccgg ccgcctccgg tccggaatgc
     4321 ctcgtgatgg aaaccaatct ggatgacatg actccggagc tgatcggcac actgatggac
     4381 gccctgctgc acgccggcgc actggacgtg ttcacaacac cggtgcagat gaagaagaac
     4441 aggccgggca tgctgctgac cgtcctgtgc gaggcgtcgc gcgcggatgt gctgaagaac
     4501 atcattttca ccgagtccac gaccttcggc atccgtcagt atgcggtgga acgcaccgtg
     4561 ctcgcgcgca ggaccgtgga ggtcgaaacg gaatacggcc gtattcgcgt taagatcggc
     4621 acatggaagg ggagcgacgt cacccggtcg ccggagatgg atgactgcgc cgccgccgcc
     4681 cgcgcgcaca atgtgcccgt gcgcaaggtg tatgaggctg cgtccaggct atgaaccggc
     4741 tcttccattt catgctgacg gccctgctgt gcgccccggg agcgcaggcg gcaaaagatg
     4801 cgctttcttt tcccgccggc cgttaccggg acggcgacgt tctcagtgtg caggaacacg
     4861 atgtggtgct gcgcgagcag ggctgggttt cctatgctct gcgcttcgaa tggccggagg
     4921 attatcagtt cgtgattctc gcccgtcgcg atggcgcctc aaagcaggtc ccttcgctga
     4981 aagtttccgt ggacgacgag gaggtgcgtc ttcagcccgt gatttccgag gcctgggcca
     5041 cctacagttt ccgctgcgcg gtgaacaagg gagagcgaaa tgttaaactt tcctctccgg
     5101 tgtcgggcgc cgacgatatt catctggcct ccgttgtggt ggtcgcgccg cgcaaggctg
     5161 cgggggtgac gctgctggaa agcagccgct cggccgagcc gctcaaggat gcgaacctgc
     5221 tgttccatga aaaactgatc gcccgcatca gggaaacgcg cacaggccag cttcgcgttc
     5281 aggttctgga caaggcgggc gagccggtga agggggtgag cgttcgcgtc tcgcagcaga
     5341 ggcacagttt ccttttcggc gcatcccagc tgactgccgc ttatgacgga actctctcga
     5401 tgcggctgcg gaacgagtac aaggagcagt tcctccggcg cttcaactgc gtcggcaccg
     5461 gagaagccct tacgtggcgc tatatggagc cgcagcccgg aagatactat tacacggtgg
     5521 gagacaagat gattgactgg gcccgggccc gcgggcttgc ggtgtggggc gaagatgtgt
     5581 attcggagtg tgcggaggaa cttcccgcat gggcctcggc ggtttcggat gcccgcatgc
     5641 ggtcggtggc cggcctgcgc gcgcgggtca tggccaaccg ttaccggggg cgggtggatg
     5701 cgcttgcggt caacggcaat atgatctcct gcggccatcc cggcgagcgg tttggcgccg
     5761 gaatgaggaa gcagcttttt cacgaaatcc gcgcatccga tccgtctgcc aaactgttct
     5821 tgaacgagga tattgatctg gacaccggcc gtccggagac cttcatgcag ccgctgcgtg
     5881 cgcttctgga gagcggcatc ccggccgacg gcattggcat acgcgcccgc tttgacaggc
     5941 agcccaacgc cttcagggtg gagcgctccc tcgacgcgct ggcgcaactg ggcctgccgg
     6001 tccggatcac cggctatgcc tgcgacgacc ccgacgaaca ggcccggctg cgcgcgatgg
     6061 aatcattttt ccgtgtggcc ttttcacatc ccgcggtgga gggcatctct gtccgcgatt
     6121 tctggttcag tgagtccgcc gggttcccgc tgctgaatgc ggacttcagt ccatctccgc
     6181 tgggtcatct gtacgagcgc ctggtttttt cagaatggtg gaccgaaacg gagggcctga
     6241 cggacaagaa cggcatcttc gaaacccggg catttctcgg cgaatacgaa gtgggcgttg
     6301 ctctgtctcc cacgcgcgaa atcaagcaga aggctgttgt gacctccggc gataatcctc
     6361 ccgtggtttt cagctactag acgctttcca acttctgaca ggttagcaag attggagaag
     6421 cgcgcctgtg cggaatccga tatatatccg gtcaacaata tattcaggga agaagcatga
     6481 ccaccagaga agacacaatg caggactatc agcggcggat agagcgtgtg ctgcggttca
     6541 tccgcgagca tcagtcggag ccgatgcgcc tgaaggaact ggcggaagtg gcctgctttt
     6601 cgccgtttca tttccacagg atattcagcg cgcatgccgg cgagaccctc agcgaataca
     6661 tccggaggct ccggctggaa agctcggccg agcgcctgtg ccgcacgacg gatgccgtta
     6721 cggaaattgc gctggatgcg ggatatgcca cgcatggagc tttcacgaag gcgttcacgc
     6781 agcacttcgg gcggaatccc tcctctttca gaaaaaccgg gaagatggat ttcgttcccg
     6841 gacgtttact gatgatgagc aaacaaaagg agacagaaat gaaagttcag atcagggaaa
     6901 ttcaggaaca ggcggcgctg gccgtgcgca ggacgggtgc ttacgacaag gcggccgaag
     6961 aggcatggaa gatcctgtgc acttatgcgg gacccagggg cctgatcggg ccgaagacga
     7021 agtttatcgg catcagccac gatgatccgg atcaggtggc cgccgagaac ctgcgctatg
     7081 acgcctgcat tacgctgtcg agcgatgaca aggggtccgg cgaggtgaag agattgacca
     7141 tcggcggcgg ccgctacgcg gtgacggtgc atgccggccc ttttgaatat ctgaagaaaa
     7201 cttatgacgg gatctaccgc gactggctcc cgtccagcgg ggagaccctg cgcgacgcgg
     7261 cttgtcttga aatctatctg aatgatccgg acaccacacc gccggcggac ctgcggaccg
     7321 aagtctgcat ccctctggag taagcgaagg tttaaaccgt ggagccgcgc cagtgcagct
     7381 tgtggcgcag cagggaataa tagttgtaat ccggcagccg gaccagcttg gcgctgtgag
     7441 ggctccgggc tatctgaata acatctccct tgctgacctc cagctgctcc tggccgtcca
     7501 tggtcagcag cagcgttttg gaggcggcca ggacctccac ttcgatcacg caggtgtccg
     7561 gaatgaccag gggccggttg ctcagcgtgt gagggcagat tggagagagc acgaaggcgc
     7621 gcacctccgg atgaatgatg gggccgccgg aagaaagcga atgcccggtg ctcccggtcg
     7681 gcgtggagac aacaatcccg tcgcaaacat aggatgagac ttcttcccca tcggcccgca
     7741 cattcagcgt cacgacccgt gaagaccggc cccatccgat ggccacatcg ttcagggcaa
     7801 tgtattcctt tttgctcccc gaaagcgaac agacggcaag cgtgcgttca gatatgccgc
     7861 attttccctc ctgcacacag cgcaaggcat gttccagctt gtcttcggtg acggtggtca
     7921 tgaacccgag acttccgaga ttgacgccga gaatcgggac atcgcatccg ttcagaatgc
     7981 tgatggcgcg cagcacggtc ccgtccccgc ccagcgccat gaaaagctca atctccgcga
     8041 ccatccggtg gcggctgatt ttcctgatgg agggcaggct ttgggcggtg tcgtcgcagc
     8101 agacaagttc tgcccccatt tccccggcgg cggccgccag ccgttcaaga acagcgggag
     8161 tatgaggttt tctgatgttt gctgttacgc cgattttttt catttctttt tccaccatgc
     8221 aaggaattcg acatttcccg ccggccccct gatgggagat gcgcagacgc ccatccattc
     8281 caggtccagc gtgccggttc caaactgttt caccgccgcc accacctccg cgcggaccgc
     8341 ttcgtcgcgc accaccccgc cgcgctgaac cttttccctg ccggcctcaa actgcggctt
     8401 gatcagtgtc acgagctcgg cgcacggctg caataatgct gtaacagcgg gcagtatctt
     8461 agtcaaggaa atgaaggaga cgtccacgac ggcgaaatcg gcccgctcgg ccagatcctc
     8521 gggcttaaga taacgcgcgt tgaccttttc cattacaatc acgcggggat cattgcgaag
     8581 ctgccagtga agctggcctg cgcccacatc aatggcgtag accctctgcg cgccgtgctg
     8641 caggaggcag tctgtgaagc ctccggtgga ggccccgatg tcaaggcaga cccatcccct
     8701 gacgtcaatc ccgaacgcgg aaaaggccgc ttccagcttc tgtccgcccc gaccgacaaa
     8761 cggcatggat gccttgacgg tgatttcgat ttccgggtcg aattcgtggc ccggctttgt
     8821 ctgcggatga ccgttcacca gcacctctcc ggcgcggatg aggcgctggg ctttttcccg
     8881 gctttcggcg agcttccgtt ccaccagaag ctgatccaga cgcatgcggc tcatttcccc
     8941 tgcgatttct tcctgtgatc aggaaccggt tttcccagat gcatgagcac cgctttcatg
     9001 tcttcccaga ccggggcctt gccggatgca ttgcgcagga gataggccgg atgaaacgtg
     9061 ggcatgacat caatgccttc aaaactgcgc cagtgcccgc gcagctttgt gatgccggtc
     9121 tgctcgccca gcagcccctt cacggccgtc gcgccgagac acacaatgac cttcggcttc
     9181 agtatcgcaa gctgccgctt caggaagggc atgcaggcct ccatttcgtc ggggaagggg
     9241 gtgcggttcc cgggcgggcg gcatttcacg atgttgccga tccagacttc gtctcgggtc
     9301 agacccatgg ccccgatgat actggtcagg agctgtcccg cgcggccgac gaatgcgcgg
     9361 ccctgcttgt cttcatctgc gccggggcct tcgccaataa agacgatttc cggccgcgga
     9421 tggccctgtc cggccaccgt gtttgttcgg gtgggcgcca gcgggcattt cgtgcaggcc
     9481 gcaatttcct tttcgatggc cgcgagaccg ggatccagaa cctgcggggc tgaagccgcc
     9541 gcaggcctcc ggtccggcgg cggttcggga cgacgggaag aaggcgcggc aaaaggcgca
     9601 gtcccaagag acttccgcgt ttcattcgac acttcaacac gttttcttcc ctcggctttt
     9661 tcctgaagaa gccaggtttc agtgctgtcc agtacttcaa tgaatttctg tctacggctc
     9721 atggcggcat ttcctttgat ccggtgagac tatccccgct atgcgggggt tgcaaggttt
     9781 aaccccgaag cctgttcagg agcgactgca tgtcttccgg aacgggcgcg tgaaacttca
     9841 tcggctccct ggtgaccgga tgggggagcg tcagtgtttc cgcatggagc atctgcctcc
     9901 gggcatccag cagatttccg gcccggccgt atttccggtc tccgatgacg ggatgtccga
     9961 tatgcgccac atgcacgcga atctggtgcg tgcgcccggt ctccagcgtg atgcggagca
    10021 gcgccgcccc ggcgaactgt tcctccaccc ggtagtgcga gaccgcctcc cgccctccgg
    10081 aggcagggcg cacggacatc ttcttgcgat caccccggct gcgtccgatc agggttgaaa
    10141 tggttcctga aatcctctcc atccggccgg ccaccagtgc gaggtactcc ttatgcacct
    10201 gccggttctt gaaggcgttc accagaaact gcatggcccg ctcgttcttg gccgccacca
    10261 tgaccccgct ggtgtccttg tccagccggt gaacaatccc gggccggagt tcccccccga
    10321 tgccctcaag gtcgcggcag tgatgcagca gggcgttcac cagagtgccg gtgtaattgc
    10381 ccggggccgg atgaaccacc aggccggcgg gtttattgat gacgatgatg tcgcgatctt
    10441 caaaaagaac atcgagcgcg atgtcttcgg cttccacggt gaccggtttc gctgccggaa
    10501 tttcatattc gatttcttcg ccggcggcca gcgcatgctg gggcttgcac ggtgacccgc
    10561 cgattttcac atggccgtcc cggatgagct gctgccagcg cgaacgcgag cagccgggaa
    10621 gggtgcgtgc gagccatgcg tcgaggcgca tgccggcatc agacgggctt gctgtgagtc
    10681 tgctcaaggg acggctccgg tttgtcataa cgcaggatca tgatgataag cccggccacc
    10741 agcaggatgg cgctgaccag ctgcgcaaag gtcagcccca tgccgtgcag gcgttcatct
    10801 ccgcggaaaa actccatgct gaagcggagc aggggataaa ggatgaaata cagcgcgacg
    10861 gtggcgccgg accgccgtcc gcgcagataa aaccagagca gcaggccgta cagcgcaagg
    10921 ttgccggcgg tttcatagag ctgcacggga tgaagggtcg ttcccggatg catcaggtcc
    10981 ggcggggagc cggccgggaa gcgaactccc agcgggcagt ctgtcgggag cccgaaacag
    11041 cagccgttca ggaagcatcc gattcgcccg gcggcgtgac cgagcggcac tccggttatg
    11101 gccaggtccc ccagtgtcca taccggaatc ttctgaacgc gcgccatgac aatcaccgcg
    11161 gccagcgcgc cgaagaaacc gccgtaaaat atcaggccgc cctcgtccac gcggaaaaca
    11221 tgcagcgggt gctgcgcaaa ataagacaag ttggccagaa catatgccac ccgcgcgcca
    11281 agaattccgg agagcatgat gaagaaagca agattggatc caaagcccgg ggggagcccc
    11341 cggcgccggg cgagccatga ccagtgcgcc gttgccagca gaaaccccag cgccaccatc
    11401 accccgtacc agtaaatggg ccggctcccg atatgaaaac atatttggtg catattgctt
    11461 tcctttgatg cagagtctgt ctaatatatc agcggtgaca gactttcaag caactatgcg
    11521 gtccgtgaaa tggccctgaa agcagataaa gtcagttcga tgctgtgcag tcactgcgga
    11581 gccgccatgg atgtgtcttc acaggacccg ttttccacgg cgctgtgccc ggcttgcggc
    11641 cgttcgcaga tagtgccgat gcagttcggc tcgatccttc ttcttgaacc gcttggggcc
    11701 ggaggcatgg gggccatcta ccgcgcgctg gatcccatgc tggaccgcta tctggccatc
    11761 aaggtgatgc agaccgccgg cgcggcagat gatcagttcg tggagaattt cttccgtgaa
    11821 gcccgcgccg ccgccgcgct caaccatccc cacatcgttc agatttattc ctgcggccgc
    11881 gagtacggcc agccctacat cgccatggaa ctgctggagg gagggaccct cgatgagatg
    11941 atccattctt ccggcaccgt ggacgagctg gatgttctgc aaatcggcat atctgtttcc
    12001 gagggtcttc gggctgcagg cgaaatcggg ctgacgcatg gagacatcaa gcccgccaat
    12061 attcttttcg acagccatca caaggccaag atcgccgatt tcggactggc ccgcttcatg
    12121 cagcagggct cctccgctga aatatgggga actcctttct acattgctcc cgagaaactg
    12181 cggaagcaga aggaggatca gcgttccgat atctacagcc tcggcgccac gctttttcac
    12241 gcactgaccg ggcagccccc gtttgacggc aagacggcca cggatgtggc ggtcgcccgt
    12301 ctggaccagc cgccgccgga tgtccgcgac agaaatccgg gaatatctcc cgaagtggcg
    12361 gcggtcattg cgcgcatgct caaggagaat ccgcacgagc gatatcccac ctatgcttcg
    12421 ctgattgccg acatgaagag tgtgctggcg gtgcttcggc agaaacgcgg cagccggccg
    12481 atgcgtccta aaatgaggaa atcgcacgcg ggcgcttttg tggccgcggc cctttttgcg
    12541 cttattgccg cggggatatg gatgagccag cgcgaaggtt cggggcctcc cgagggtccg
    12601 ctgtccggcc cgtctcgtcc ggaggaaccc gccgtgccgg agatgcgggg aagtaagcct
    12661 tttgagggcg ttcgcggaac cgccatggaa cggatactga acctgttcgc cgccgggaat
    12721 gtcatggcgg catcagaaag tctgcaggcg ctgtatgaaa aaattccccc ggacagtccg
    12781 gatcgctcgt ggatatgcct gtttcaggcg ctgatgctct ggtatgacgg aaacggcggg
    12841 agcggggatg aactgcttga ccgggctctg gccgggttcg ccgggagcga tgcctcggcc
    12901 ggcgccctga tcgccgcata catgaagggg aatatggatg aggcggcggt tgaagcctct
    12961 gccggcgagc aaccgcagtg ggtgggcgat ttcaccggat ttgtcgcggg tatgaatcat
    13021 gcccggcagg gacgaaagga tgaagcggac gtgcgctggc gcacttatct tcagaccacg
    13081 aatgacaagc cggcctgggt ttatgtcctg aagcctctgg cgcagaaatt cacggaaagt
    13141 cacgcccgct gggaacgtct gaatgcggaa gtgaccgctc tgctcgaagc ccgcaaaccc
    13201 ggagaggccc gggttcttct gcgcgatttc aagctgaccg ccggtccggt ctttgccgct
    13261 gaaacggatc agctcttcga gaaggttcag gcgctggaag cctcgctgac cgaggccgag
    13321 gcggcccgcc tcgcggaaca gcaggctgcg gccgccgcca gaaaaaaagc aaaagccgag
    13381 tccgaggtcg caaaggttcg gaaagcggtg gaatccggcc ggagacacta tccctccagg
    13441 gatttcgata gggctctagc cgcaattgaa aagcttcggc ccgcgatgac tacggaagag
    13501 ggatgggtgg aatatcagct ggcgcgcgaa aaagtgtccc gtcttgacgc aatgcaccgc
    13561 tatctccaga acgcattgcg cacgcgtccc tgcagaatca gtgtgttcgg gaaaaacacg
    13621 cgcgtggcgg gagcaacctc gcgtggtgtg cgcgtggcga ttggcactca ggccgatatg
    13681 gtgcgggagt ggcaggagat cacggcggcc cagtatgtcg gcctgattcg ccagtatctt
    13741 gatgctgaac agaaaaccgt gcgcgagaaa gcggatgatc atctcagcct ggccgtgttc
    13801 gcttcggaaa cagacggcgg acagctggcc gcgcagcttg cgcgaaaagc cgtggcgctg
    13861 gatccggccg tcagggatct cggcctgcgc cttctgcctg atctggactg ggaatagcga
    13921 ggctgaactg gaggcggggg taaaaacatg gcggggacga cgggactcga acccgcaaca
    13981 cccagatcga cagtctggta ctctaaccga ttgagctacg tccccgccct gtgacggaaa
    14041 tttccagaag aatattcatg gagttcatct tttcaagcac aattctctgg aaatctctgt
    14101 ttaagtttcc tcccgccgga ttatattgtc ggcatgaagg ttccctcgag aatccttgag
    14161 aagctcaaga agcttccgga cagcccgggt gtgtacatta tgcgcgaccg gacgcgccgt
    14221 gtcatttatg tggggaaggc cgtgtccctg cgcagccggg tgcggtccta tttccagccc
    14281 ggcacctatc gcaaggcccc ggtcaaggtc cgcggcatga ttaacagcgt ggccgatttc
    14341 gattttcttg aactgcgcac cgaagaggag gccgccctga ccgaaggccg cctgatcaag
    14401 gattacaagc cgcgctacaa tattctgctg aaggacgaca agcgcttccc gctgatccgc
    14461 gttgacctgc gcgagcggtt cccccgcttt gagcgctgcc gcctgcacaa ggaggatggc
    14521 gcgcattact ttggtcccta tgcaaattcg ggcgccgccc gcgcggcccg cgagtttgtg
    14581 gaacgccgct ttggcctgcg caactgtccg ccggcgagcc ccgacgcgga gacatacagg
    14641 cactgccaca atgatgttat ccgctactgc tccgcgccgt gcatcggaaa ggtctcccgg
    14701 gatgaatacc tgcagcgcgt ggaagaggcc tgcgcgttcc tgcgcggcga gcgggtggaa
    14761 tatctccatg aagtgcgggc cgccatgcag gcggcctccg agaagctgga tttcgagaag
    14821 gcttcggctc tgcgcgacac gctgtttctg ctgcaggagg ccgtgaagcg ccgcagcctt
    14881 gcccggaaga ccccgctgat gaaacaggaa tccgctcggg ccggagtgct tgagctgcag
    14941 gcggcgctga acctgccgcg cccgcccgcc gtgatcgaag cgtacgacat ttccaatatc
    15001 tccggcacct ttgcggtggc cagcatggtg agttcggtga acgggattcc gcagcccggc
    15061 cgctatcgtt tgtaccggat ccggacggtg cagggctcgg acgacccggc aatgatggct
    15121 gaggtggtga agcgccggta cagccgcctg cagcgcgagg gccgggacat gccggatctg
    15181 gttctggtgg acggcggcat cacgcaggtg cgggccgccg cgcgctcgct ccgggaactg
    15241 ggcctttcgg acatccccgt agcaggcctg gccaagcgtt ttgaggagct cgtgcaggac
    15301 cgggcgggcg gggccgcccg tgtgcttctg ccgctggatt catgcgcgct gactatggtg
    15361 cagcagctcc gcgatgaggc ccatcgcttc gccctgactt ttcatcgcag aatccgcggc
    15421 cggcggatcc gtgaatccat cctcgatgac attccgggga tcggccccgc ccgcaaggcg
    15481 ctgctgctgc gtcattttgg atctgttcgc cggatgcaga aggccactgt ggaagaaatg
    15541 gcccgtgtgc ccggcctggg ccgggccatg gcggaactgg tctgcggccg gctggcgaaa
    15601 gacggatgaa acgcttgaac ccgccgtccc cgctgtgcta gcttgacact gttcaggagt
    15661 aatcccttgt ctgaaaacgg cgtaatagaa atacaaacgg atcgtgttga gcggctggcc
    15721 tgttcgcagt gcgatgcgcg catggatgtt tcgaaaattc cggccttcac gattgccaaa
    15781 tgtccgcagt gcggagcccc gatgcctgtg cccgcccgat tgagccagtt tctccttttc
    15841 cgggaactgg gcaagggggc catgggcgcc gtgtatcagg cgtttgacac cacgctggcc
    15901 cggcatgtgg ccatcaaggt gatgagcagc aaactcggag caaaccgggc gtttgtcgaa
    15961 aggtttttca aagaggcgcg tgcgctggcc gccctgaacc atcccaacgt cgttcagatc
    16021 tattcctgcg gggaggagaa gggccagccg tacattgtca tggaactcgt tcccggcggc
    16081 cgcgtggatg cgcttatgga ggagagccgt atctccgagc ttcgcggcct ggaaatcggc
    16141 gttgatgtag ccagcggtct caaggcggca tccgatatcg gcctggttca cggtgatgtt
    16201 aagcccgaga acattctctt caatcgcgac gggctctgca aggtggtgga tttcggcatt
    16261 gcccgctttg cgggcgagca gagcgatggc ggcgggattt ggggcacacc cttttatatt
    16321 gcgcctgaag cggtgcgcgg acaggccgtt gatcagcagg ctgacattta cagcctgggg
    16381 gccactctct tccatattct ggccgggcgg cctccgtttg atgcggagac ggctaaggaa
    16441 gtcatactgg cccggctcaa atcgccggcc ccctccctca cggaattctg tccggagatc
    16501 catccggaaa cggcgcgggt ggttggccgc atgctggagg aatcttccgt ccgccgctat
    16561 cccacctatg aatcgctgct ggcggacatg cgatcggtgc ttgaacgggt tcgcgcggaa
    16621 tccagagtca ggccgggaag ccggaagaaa aaaaagcagg ccgggcatcc ccatgccgtc
    16681 accgcggtga ttgtgaccgt tctggcactg ggcattctgg gcgtgatctt cgccggcatg
    16741 aagaatgcca gaaagagtgt ttcccgtcag ccggctgttc cgcaggtccc gccgggcacg
    16801 gtgctggtca agccgtcttc ggccgcatcg ccgtcgccac aggctccggc cgcgtccgcc
    16861 ccggcggccc gtccgtcttc ccccgccgcc gcgtccccgc cgcaggcggg tgcgtttgaa
    16921 cttcgcctga ccacaaaaga tgcaggcggc gcggatgcgt atgttcaggg cggcaagagc
    16981 ggcggagaca agaatttcgg acaggaagat gtgctctggc tgaaatccaa cgatcaggtt
    17041 ccgattcatc tggtcagaaa agtttatctg cgttttggtc tggatgatct ggccgaacgt
    17101 aaggtgcgtg acgcggaact caagctgacg gcgaccccgg gcggttccaa caggaacgat
    17161 ctgtataaaa tcaagctgtg gggtctggcc aagccgtctt cgaaaggcga ctgggccgag
    17221 attgatgacg ggtcctttga tgcatgggcc aaccggctga cctggaacaa cgcgccggga
    17281 aatgatttca ggagtcccga tggcatgtcg gaggatgcgg tgcttttgtg cgttgtgaat
    17341 gcgcacccga atccgccggt cggcgcggcc gtgtcctttt ccaatgccga ttcagtcagc
    17401 aaggatgccc tggtggactt catcagcgcc cgccggggaa gcgtgacatt catgctgacg
    17461 gcggagggcg gtccgtcaaa tgtctacgga tggaagttcg catccaagga gcatgcgtac
    17521 ctcaatcctc cggaactgac ggtgcgcggc gaatagcctg accgtctgcg gcgtctttga
    17581 aaagttccaa tgattggaag tttttgattc aaaagttcca atcattggaa ctttttgcgc
    17641 agaaacttcc aatggctgga acttttcccg ccgcctgaaa ccatgcggct caggcggcct
    17701 gagtttgggt tccgcgctgg aggcagaggc gcaggatcac gatcaccggc caaacggcaa
    17761 gcacggcagc cgtgccgatc cacagacgcg ggggcagctt ggccatcaga tcgccgccca
    17821 cccacagcca gacaaagacc ggaagaacta tctggagcag gttcgctccg ctctgtccga
    17881 tgtcgtggaa gcgatgtgag agcaccagca gggcaaagta gaatgcagca gccgtgacag
    17941 gcactgcgat ccagccggcg acgcgcggcg gtgtctgtat gcggttccag taggccagag
    18001 caaggatgac cagcagcggg agaaaacgcg taacgaaagt gcccctcccg atgcttttct
    18061 ggaactgcgt ttcaacggcg atgcagcggt ccctgagcca gagctggcgg ggaatgggaa
    18121 tatgggcttg aaagtccgtc atgtctgaac tcctattctt cgatggcttc tttttcggcc
    18181 ttttgctgtg ccttgtccag cgccttggct tcagccagta ccttcagcgc aggtgtttcc
    18241 cggcctttca ggttgattgc attctgggcg ttttcaatag cggcctgcac atcgcccgcg
    18301 gcgagagcag tttcggccgc ggcgacaaac tgatcatgaa cgttatctac gatctgcatg
    18361 gcctgttcat ggccgggtgc cagccgcaag acgcgctggg cctggtccgt tgcgtccctg
    18421 gtgtctccgg cctggaaggc cgactgggcg gcatcggcat agtgcgcgat ggcaacgggc
    18481 ttcatggtgc ggtatttgct gatgccgaat gcggcgccgc tgatcaccag caggaccata
    18541 aagccgatga cggcccggct tgcggcggct ttcttcttct gccggagcag aagggaaagc
    18601 atggcacatc cgccgaagaa cgcaaagcag gtcatgatgc cgaaccaaac agtatagttg
    18661 cccggcaggg aaacgtcgag cagcagaccg gtgatgaaca gtccgagcgc cgcgccgtaa
    18721 tactggaatg agtcaattac gcctgaagcg aatcccgcca ttttcttgcc gccgatatcc
    18781 accggcgccg cggcgcccac gatggagtgg gtggagttgg ccgtcactgc gatcaggacg
    18841 aggaaaagac acccaagaag gatgccggcg gttgtcggac cgatgatgcc cagtgccatc
    18901 acggtggtgg cggcggcgat gacgcaggct tccccataat agagcaccat cgcgacggga
    18961 tagcggttgc cccggaagaa gcggtcggag gcccagccgg atatcaccga gccgcagacc
    19021 gccacgatgg gcaccagtac gagcgtgaag ctggcaatga gcggcaggtt ggtcttcatg
    19081 ttgaagccca gcacatcctc aaagtagagc ggcgaaagct ggtcggcggc gtggcggaca
    19141 gcgccggtgc aaccgtaggc cacggcatag aaccacacca gcggatttga aaacacgaac
    19201 ttaaacgact ccagcaccgg cacggtggtg ccggcggaat tatccacatc gtcctgaatg
    19261 gtgccatgga agccggcatc atcgggcgac tgcgcggaaa tgaaatagaa ccagatggcg
    19321 accaggccga ggaaaatcgg cgggatggtg aaaagcatgc gccactcgcc ggggcccacc
    19381 acgagggcga ggaagctgaa gcccgcaaga atccacggag cgagcatgga gatcatggcc
    19441 tggccggact ggatcatgaa cccgaagatg cccgagaaaa gtccccgctc ctccttgttg
    19501 aaccatgccg cgttgatctt gaccatgccg ggagtgccgg cggcctgaat ataaccgttg
    19561 accatccaca ttgcggcaaa ggccatgaac gtggaggtcc agggggaaaa tccgatcagg
    19621 acattgacca aaatggatcc aatggctccg atcagcattg aggcgcgccc gccgatgcgg
    19681 tcgcagaaca gaccgttgac cagctgcccc gtgccgtagg cgatggaaaa ccatgcggcg
    19741 atgttggcaa tgtccccggt ggaccatccg aattcagcgc gcatgccggc gccggcaaag
    19801 cggaagttgt agcggcacat gtaaaacatg gcatacatga ggccgaccgt aaaccagttg
    19861 agtcctcgac gagtcttgaa tcccaagggg tgctggtgct gctcgttcca ttcccgtttt
    19921 gcttctgctg ctatggtcac cgctcaactc ctttttgtca ggattattgg ctgtctctta
    19981 acgccagaaa tactgggggg catcataccg gttttctgac ttctcacaat gaggaattct
    20041 taaaaagttg cggcgagccc gccaataagc tatcagtttg cgtgaatttt caggagcgat
    20101 gaagaagcat tatacaacga aaagtcccga ggagacctgc tccgtggcgg ccgcgctggc
    20161 ggcagagctg cagccgggcg ctgtccttgc gtttcacggt gaactgggct ccggaaaaac
    20221 ctgttttatt cagggactgg cggccgcgct gggtgtcgaa gcggctgtga gcagtcccac
    20281 ctataccctt gtgaacgaat accgcggccg cctcccgctg catcacatgg atctgtatcg
    20341 ggtggccgat gccggagagg cgcttcatct cggactggat gagtacctgt atggcgacgg
    20401 ggtgacggcc atcgagtggc ctgaagtcgt tgcaggcctg ctcccttcgt cggcgctgca
    20461 catctatctg cgcatgggga ttggtcttga cgaccgtgag atattttttc ggactggaga
    20521 ggagccgccg ccatgaagat tctggctgtc gaactttccg cttttcaggg aagtgcggct
    20581 ctgtccgggc cggatgactt cagtctgcag gaagtctggg accaggagcg tggccggcat
    20641 gagggcgtct tcagccggct gtcggcgctt ttgtcgcagg cgggatgggg atggggcgat
    20701 gtggactgtt ttgcagccgg ccgcggcccg gggatctact ccggcctgcg cacggcgttg
    20761 acctgcgccc agactcttgc tctgcccgga gagaaatcgg tctactgcgt cagcagcggc
    20821 gcggtgctgg cccgcggcgc gtcgagggaa atgaatgcct cccgggttgc ggttgtcggc
    20881 gatgcgcgcc gcagcctgct ctggtacgga gtttttgatt tttccctcag tgaaaccgat
    20941 tcccccgcgg aatggcagat ggtgaagtcc tcggaacttt gcgcccgcct gcctgccggc
    21001 gtgcccgtgg tcagctccga ctgggaacgg ctggaaccga acctggcggc gcagggtctt
    21061 gatgggctta actggatcag ggggaaccga tatccgcgcg cccgcgatct ggcagcgctg
    21121 gtgcgcctgc gcatggagca gggccttccg tctgaaaaac cggttccggt ttacatgcac
    21181 cctcccgtgc tgattgcgcc gcggtttccg ccggcatgaa aaaaacgccg cggtgagaaa
    21241 caccgcggcg tttttctttc aggttattct cagtattcga agccgctctc gccgatgaat
    21301 tcgcggatca gcgaagtcgg cggcaccaga gagctggaag ccgcgttgaa gcgcgacagg
    21361 aactccagca gccggcgggc atctgcatac tgcgggtcgt agggcttgac ctcggccagc
    21421 aggggctcca catacggttt cagaacggcc agttcatagt cgagcgcgga attgatggcc
    21481 gtattggcct ccgcctgata ggggaatatc cactttttct ctcctcggcg cacgctgggc
    21541 cacattttca gcgtcatcag cgcgggattc ccgcgatagt tgtgatcgcg gatcatgcgg
    21601 cggatgagcc ggttgtcggt cgtggagacg cggttgctgt agtccaggtt cagctgcgtg
    21661 agcgcgctga tgtatatctt gtatttgtga tccggactga tggcctcggt cagccgggga
    21721 ttcagggagt gaatgccttc gacgacacag atctggtcgc tttccagctt catggtcttc
    21781 ccgcggaact cgcgggctcc ggccccgaaa ttgaaggtcg gcagctccac ggtttcgccc
    21841 cggtccagca ggctgaggtg ctcgttgaac agcttgagat ccactgtctc gatgttttcg
    21901 aaatcgggtt ctccgttttc gtccatcggc gtgcgcgtgc ggtccacaaa atagtcgtcc
    21961 accgaaatgg ccaccgggcg cagtccgttc acgcgcagct gcacacaaag ccgcttcgcg
    22021 aatgtggtct tgcccgacga agacggtccc gcgatgcaga tccacttgat ctttccgccc
    22081 tgggcggtga tcgtgtccgc gatttccgcg atcttctttt cctgataggc ctcggaaata
    22141 tggatgaaat ccctgagttt cccgctggcg atgatcgtgt tcaggtcgcc gacggtgcgc
    22201 acgttgacga tgcggcccca cgccttgtag gacttgaaga tgtcaaacag atagggctgg
    22261 cgctcgaacg gagacatttt gggggtctga ttgtcgcggt ccgggaactg gatcacgaat
    22321 ccatcctcgt acgggaacag tttgaagtgc tgaagcgcgg acgtgcggtc ggccagcggg
    22381 ccatggaaga gatccatgta ttcctcgcac gtgttgacca ccaccttcgg gggattgctg
    22441 tagcgaagca ggttgaccgt atcatgccgg ttctgccctt caaaatggcc aatggcgtcg
    22501 ctgtattcga ttttgcgccg gacgataggc aggtcggcct cgacgatctg gtgcattttc
    22561 tgctcgatgt ccttgatctg ctttttggaa atgcccttgg tcccgttcat ctcaaaattg
    22621 cagaagtacc cgtctcccag cgaatgctcg atcgaaagca gtgccttggg gaaaatatca
    22681 taggtcgcct tggccagcaa aaaagcggct gaattgcggt agatgcgcca tccatgggaa
    22741 ttggcgagtg tcagcatctg aattttgctg tccacttcaa tcgggaatga aagcgagacc
    22801 atgtcgttgt tcaccagtgc ccccagatag ggcaggccgg cctcgttttc ctttttcttc
    22861 agaaggtccc tgacgcatgt gccgggcttg atttcaagtt tggagccgtt atcaacggtc
    22921 accacgattt tgtcttttct gatcattctt tctctcctct ttctttaaat gcaaaccgcc
    22981 cttatacact aaacccccat ccgatggaat aggggattgg aattttattt gaagccccgt
    23041 cgcgcgcgtt tattccggct tgtcgttgag gtcatcaagg actttcagcg acttcatgat
    23101 ttcgtcccac tcgggatcag tttcttcgcc gtcgcccggc gtcgagtctg ttttcccgtc
    23161 tttctcctcg agcaaaccct ccagcagagt tttggctgcg tcttcagccg gttcctgaac
    23221 agtttcacct tcgtcgccgg attcactcgc gggggcctcg acggatgtgt cggcgggcgc
    23281 agcggcggcg ggcgcggcgg caatcaggaa gttgacggtc ggattgtgaa tggtgtgctg
    23341 ttcaataata accggcagat gtgtataggt gaacacttcg cacggcatgc acaggatctc
    23401 cagcaccgcc tcgtcatcca ccttgttgcc tacaaacgca tgtacaatct cgccgttgga
    23461 gaaaaacatt tcagcggaaa ccatcccctt gcgctgctcc agctcaaggc cgcccgtttc
    23521 tttttcggtg tgcaggtaat agacaaggtc ataggttgag aattctccga acggcccgct
    23581 gttttgcccg gttgaagtgc gcaggctctg gaaatatttc cggaaccggc tcgtgtccag
    23641 ctgcggcagc agcaccacgg cgtcttcccc gcctgcggcc gccggcacag tccgcctctg
    23701 tctgcgcagc cgaacaatga acaggccttc gatgatcacg atgaccagaa gaatgtagct
    23761 ggaaatcata tacttatcga ggaaccgcac caccgtgccg gccggtgcgg cggcgggcgg
    23821 tggttctgcc ggagcgcttt ctgttgtttc tatctccccg gctgcagtcg cgacatcggc
    23881 gctcacctcg gtttcggctg caggtcccgc cgcctcggac tcttccggcg gcgcgggctg
    23941 ttcagcgagc aggggttctg gttccgaaat gccggacgga atttccggcg tggcggccgg
    24001 cagatcagga tcagccgaag gtgcagcctc ccgggccgca atctccggcg gctcggtcgc
    24061 ggccgcgggt gcaggcgctg gttgaaccgt cggcggcgcg gcaggcgcgg gttcgggaat
    24121 taccggcgct tcaggctcgg gcgcaaccag cacgggctgt ggcggggctg ccggggtttt
    24181 aacttcctta accggaactt ctatgggcgt ggaatccgcc ggccgcgcac gagcgacgga
    24241 tactggcgcg ggcacgggtc tgggcctggg aaccggtcga acaacaggcg gttccgggag
    24301 ctttgcctcg acggcacgga ccggttccgg ttgcggttct tcggcggcag gctcgggttc
    24361 aggcgggtta taggccagcc cggccgggag ggctttcgga atgctcaatt ctgcccggtt
    24421 ctggaagaat gtgtattcaa taatccgcgc atccggggtt tcctgcagaa tgcggaactc
    24481 gtcgccgggg cgaaggcgca ggaagccgct gacggcgcgc gcccgatccc cgaaaatgag
    24541 ctgcttctgc tgggtgaccc atgcaatcgg atttcccttg cggtccaatt tgccgacggc
    24601 tgcggttatg ccgtcgggga gtgcgacaat atagaattcg ggattttcct gcagcagcgg
    24661 gacgctgttc ccggggccga acatccggct ggcatcagcc accggaagac ggacatcgct
    24721 gccggttact tcgatgaagc cgtaatcctt attcctgctg tcggaacaga acccgggcat
    24781 tgcggccagg cacagcagca acagtgtcaa acatgtcgga ttggtcttca tcctgcctga
    24841 aaaactgaaa agaagagtca ttcaaagagc ccattgtgtc aatccattat tcagcggttc
    24901 tcatggtgtg caggagtgga tcatgactgc gactttgcct tgatatgatt cggtccaaac
    24961 tgctagggta cagcgcatgt ccaattcggt cgaaacagaa agcgtggttt ggcgcggcgc
    25021 atggggtttt atattcctcg ggctcgggat cattctgatg ctgagcctga tatcatggcg
    25081 gccgggcgac ctttcgctga ttcaggaccc cccgaatgag cagggcacca atctgattgg
    25141 tccagccggc gcctggatgg cttttgcggg tttcgggctg ctgggtgtga cgggctatct
    25201 gcttcctgtt gtcctgtggg tgcaggccct gttccttttt ttaaacaaga gcggaagctt
    25261 cagggccaag gcgctttatc agcttgtggt gctgctttcc ctggcctgca ttgttgaact
    25321 tcagccggat ctgtgggaag gcctgcggac tcatctcaat attttctacg ccggcggact
    25381 cttcggacat ctccttgcca ccgccttcct ggtgcggctt ctgggcgtgg cgggatgcgc
    25441 aattgtcgcg gtttgcgcgc ttctgactgc actggtgctg ctttttgaaa ttgagcccgt
    25501 acagaccctc ggaggagtcg gagtcgctct ggcggctgtg ccccgcaaaa tcgggtccat
    25561 gtggcgcggc gcgcgggacc ggcgcgccgc cattgaggaa gaggagcgcg aactcgagcg
    25621 ccagcgccgg cagctggaga aggtggtgcg aaggcaggaa cgccgccggc cggcgcccgt
    25681 ggtggaatcc gaacccgagc ccgaacctgt tcaaccgcct tctcctccgc gcgagccccg
    25741 gcccccgcgc cgggaaaaac cccgaccggc tccggttgag ccgcccgggc catccgcggc
    25801 cgattcactt ccatctccaa cccctcagta cacgctgccg gcgatgactc ttctggatcg
    25861 tccgccgccc gcgcgtgatc ggcaaatcaa agaaaatttt tccgagagcg cgcgtatttt
    25921 gcaggagacc ctggctgatt tcggaatcga ggcagaagtg acgaacgttg agcgcggccc
    25981 ggtggtgacg cgttttgaag ttctgccggc cgcaggtgtt cgcgtcgaaa gaatcagcgc
    26041 attgagcaac aatattgcgc tggccatgaa ggcggtgagt atccgtgtcc aggcgccgat
    26101 tcccggcaag ggggtggtcg gcatcgaggt gccgaactcg agcacaacgc tggtatatct
    26161 gcgcgagctg ctggagggcg aggcgtggca gaagtcgcgt gccggacttc cgctggccat
    26221 gggcaaggat gtcggcggca atgatattgt ggccgatctg tcggacatgc cgcacatgct
    26281 catcgcgggc gcgaccggct caggaaagac cgtttgcatg aattcgctgc tcgcggggct
    26341 gctgatgtcg cgcaccccgg atcagctgcg cctgatgctg gtggatccga agatcgtgga
    26401 gttttcattt tacaatgagc tcccgcatct tgttgttccg gtgatcaccg accccaagaa
    26461 ggtcggcctt ggcctgcgct gggcgatcat ggagatggag aagcgctaca agctgtttgc
    26521 caaggcggga gtacgcaaca tcgcctccta caactcgcgc ccgaaagtga cgcaggagga
    26581 gctttttgaa gaagcggcag agcctgcgga caatgcctcg aaaatgcccg atcgtctgcc
    26641 gtatattgtg attgtggtgg acgaactggc cgacctgatg atggtggcgc aggcggaagt
    26701 ggaaaactat attgcccggc tggcgcagct ttctcgcgcg accggcatcc atatgattct
    26761 tgccacgcag cggccctcgg tgaatgtcat cacgggatcc atcaaggcga atatccctgc
    26821 ccgcatagcc tttcaggttg cgcagcgcaa cgacagccgg accattctgg atgcgaatgg
    26881 agcagacaag cttctgggaa agggcgatat gcttttcttg tcaccgaaga cagggaaact
    26941 ggtccgcgct cagggcacca tgacgagtga cagcgagatc cgcaggatcg ttgactgctg
    27001 gaaggagcag ggccgtccgc agtacgagac cgccatcaag gagcagatcg aaaacaagaa
    27061 aacagagctg ccggagatga atgatgacga tgagctgctg gatccggcga ttgaaatcat
    27121 caggcagacc cgccgcgcct ccacgtcctc gctgcagcgc cgcctgcgca ttggttatac
    27181 ccgcgcggcc cgtctgatgg atgtcctgga ggaaaagggg atcatcgggc ctccgaacgg
    27241 atctgatccg cgcgagattc tgattgatct ggacggagaa atcccgcaga acccagatga
    27301 agacgaggag atggacgact gatgccccgc ataggacaga tattcaggga aagccgcgag
    27361 aagaaaggcg tgacggcctc gcaggccgcc caggccacgc ggatgaaggt ccagaccgtt
    27421 cagcatctgg aaaacaatga tttcggcaag attgccgcgc ctgcttatgt taagggcttc
    27481 atccgcctct acgcggaata tctggggctg gatccggccc cgctgattcg cgaatacctg
    27541 aatgggctgg gtgaaacccg gtcgccgatc gcacccccgg tgaagacttc cccgtggcgc
    27601 gacgttctga aaaaagcagc aggccgattg tcagccagcc tgaaaaaaat atctcccgcg
    27661 gtctggatta agctggccgg cgcggctgtt ctcctgcttc tggcatggtc gctgttgctg
    27721 ggcatttcgg cggccgtgcg ccggaaggcc gcaggcgaac cgccgtccga ggaattcact
    27781 gcccgccccg aagccgcttc cggatttctc gaggatattc ccgaacccta ctgggatgtg
    27841 ctcgatcctt cttcccggcc cgttcaagat ggagctgctc tatgaaatgg aacattccga
    27901 atattctcac ggtcagccgc ctctttctgg cgcttatttt gctcatgctg atcggcatgc
    27961 cttatccgct gacaaagacg ctggccctgc tggtgttcgt cgccgccggg atcaccgatt
    28021 ttctggacgg acatcttgcc cgcaatgtgt acggcgtaac ggcgtttgga cgtctcatgg
    28081 acccgctcac cgacaaggtg ctggtctgtt cagcgctggt aagttttgtt ggcattcgcc
    28141 tgccgggatg ggaccgtccg ctgcttcccg tgtggatcgt ggtggttgtt atttctcgtg
    28201 aattccttgt gacgggcctg cgcctgctgg cgtccagcat ggggcacatc atcgctgcgg
    28261 gccgatgggg caagcataag accgtgtggc agattatcgc catctcggtc attcttctgg
    28321 gtctggcggt gcgctatgac gtcctcggcc atttgtcgcc ggcggctcta gaaaagtatg
    28381 actttgcctt tggtttgatc gcgtatgccg tcggtctggc ggtcaccatg atcactgtgg
    28441 cttcgggagc catgtatttt gccgagcaca ccgaactgct tcggcacaag atccaataaa
    28501 cagtttgtgg agaaaatcat catgaaaaaa tgcttcgccc tttttgctgt gacggcggcc
    28561 accgctttcg cgcaggcccc ggaggctgtc gcgcagcgca tgtcgcttgg cgaaatcatt
    28621 gaaaccggcg gctggctgat gtatgtgctc ggggccatgt ccgtggccgg tctggccatg
    28681 attatatatt ttttcatggt tctccgcccg gaacgggttg cgccccggag ttttctgaag
    28741 gaactcagca cggagctgaa gcagcgccgg tttgaccgcg cccgggaact ctgccgctgc
    28801 gaagaccacg cggcggcctg tgtggccctt tcgggtctca atcacctggg cagcagccgg
    28861 gaaaccgaac ccatgctcct gaaggagatt atggagggcg agggcagccg tcaggcctcg
    28921 atgcttcagg ggcagatcca gtatctcatg gacctgggcg ttctttctcc gatggtggga
    28981 ctgctcggca ccgtcatggg catgctgcgc gccttcaatg cggtcgcgct tgacattgcc
    29041 aaggccaaac cgatgattct tgcggccggc gtgtcgcagg cccttattac aacggccgcc
    29101 ggtctgctgg tgggcattcc cgccatgatc ttctattcct atttccgagg ccgcagttca
    29161 cggctgatcg cctccctgga agccgtttcg gccgatcttc tcggcgagct tctggaagcg
    29221 gagaacctcc catctgacca atccggatcc aaatgaattt ccgcagacag aaccggcctg
    29281 aggccctgag cttccagatt gcgccgatga tcgacgtggt tttcctgctg ctttgttttt
    29341 ttatcacttc gcaggtgttt tcgcagtggg aaacggagat agacatcgca ctgccgaccg
    29401 caaagacggg agaagtcccg cagcgcctgc ccggtgagct gatcattaat atcctcgccg
    29461 acggccggac cgtgatcagc ggacaggagc agccggagga ccgtctgggc ccgcttctca
    29521 agcgtctttc aggattcttc ccgggtcagc ccatcatcat tcgcgcggac cagaagacgc
    29581 cctatcagca cgtgatccgg gtgcttgatc tctgccggca gagatcaagc a
//
LOCUS       DAOQWR010000014        29459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1855_length_29459_cov_5.696096, whole genome shotgun sequence.
ACCESSION   DAOQWR010000014 DAOQWR010000000
VERSION     DAOQWR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 29459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29459
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1855_length_29459_cov_5.696096"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PKK36_02250"
     CDS             complement(<1..1134)
                     /locus_tag="PKK36_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR93409.1"
                     /translation="MKRTFNGKIHTRTAFPVPYGASVLNDGVCFTLCSRHARRVWLML
                     FDEPDAARPSREIELRPELNRVGDIWHIHVEDARAGQFYAYRMEGPADGPEARFYNPR
                     QWLLDPYALAVSGAPKWADPHGLKPGDQPLNGAAFPRGVILDDTFDWEDDQHPSTPLH
                     QSVIYETHLRGFTVHPSSGVSYPGTYRGFEEKIPYLRDLGITAVEFLPIQEFNEMEYY
                     LENTSRKSLRNFWGYSTLAFFAPNARYAASGGRGGQVREFQEMVKALHKAGIEVILDV
                     VFNHTAEGGDGGPTYSFRGIDNSIYYIMEKNGRHYANYTGCGNTVNCNHPVVRDFILD
                     CLRYWVLHMRVDGFRFDLASVLTRGTSGDVLPNPPAVEHIAEEP"
     gene            complement(1118..2329)
                     /locus_tag="PKK36_02255"
     CDS             complement(1118..2329)
                     /locus_tag="PKK36_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HNR93410.1"
                     /translation="MFRRIWDIAVRIVWVISFALIAMAAVELLRLYVLLRDLHAGAAA
                     GYLFIVGVVSVSLVVWLLHQWLSFPAVLTPPELPENIAEASHRQLTSYTRYLIRYLRR
                     LSGNPSLSPEQAAAAEQQMALLQDTLRSHPLRDDLIRDTRTAEEQAIRPLLAVLNGLA
                     AHEVRRSVRDVMLGVTLSPYHSLDAAGVLYRNGSMVVRIARIYNARPLHREQWMILRD
                     TLRTVAAVNLLNLGKTLFDKLFSGIPFVGRVMDDIAQGIGAGLLTSVAGRAAMDRCAA
                     YRGWTRARAAESITAHAGNFFTDVKDMFTKDVLPSLRPRIMVTAPQAAGEPDFWKRLN
                     DGITGAVDSSAKAVGDYLLKPAVAGTIGVARFTAEGSYHTIRGTGRGVKKLFAWFVPG
                     HKDTKENEKNI"
     gene            complement(2388..3164)
                     /gene="recO"
                     /locus_tag="PKK36_02260"
     CDS             complement(2388..3164)
                     /gene="recO"
                     /locus_tag="PKK36_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNR93411.1"
                     /translation="MSRILKTDAIALRIAPVSNTSSVVTWLTPGWGRLSTMIKGGRRP
                     KSRFLGQYDLFYTCELLFYGREHRELHIASECCPLKMRPALRTDWRAAAMASYFADLA
                     ARIGPVHAEHADTWRLLDEALDDLAARGAAAEHTFWLEMKLLESMGLAPRLQDCLHCG
                     RELEPGRDDTWFSHERGGLICRTCAGTCPEEPARLAPDIFAALRGLQRARHSSGARAV
                     RCQPRQAREIEGLLGRFLSWHFEIPLTGRDIAFRILRASL"
     gene            complement(3164..4546)
                     /gene="mgtE"
                     /locus_tag="PKK36_02265"
     CDS             complement(3164..4546)
                     /gene="mgtE"
                     /locus_tag="PKK36_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNR93412.1"
                     /translation="MKQLKREVRRLIREEQWNQLAEMLDSRRAPDLAELLQKLLPEER
                     VRVFALIDEEFKPDVLAELEGLAEDEVLESLSNNELAELVEEMDPDDAADVIAELPEE
                     RSEEVLELMEEEESRDVRNLLKYDENTAGGIMTPEVVAVRENMTVAEALDAIAFMEGE
                     EAFYYAYVVDDDQKLKGFIGLWELLKIRNRQITLGAIAHKDVVSVKADTDQEEVARII
                     SRYDLLAVPVTDADGQLLGRVTVDDVVDVLQEEASEDIFRFAGSDDSELTSMTPLHAC
                     GARLPWLLITLATGFVTSFIFKKFLNDISQVMVLSFFVPVVMAMGGNTGIQSSTLVVR
                     GLALGTLEGRSMLKVLGREISAGAIMGIACAAIIGLWARYLIHLSGHENDLHTPLFLS
                     LTVGVALFSAMTFAAVFGAMVPIALNRLRVDPAVASGPFVTSSNDIFALLIYYSIALI
                     LLGLRSGTGG"
     gene            4748..7888
                     /locus_tag="PKK36_02270"
     CDS             4748..7888
                     /locus_tag="PKK36_02270"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNR93413.1"
                     /translation="MSTSKSEIRARWKPGAAVLGAALAVYYLTLSRVFYPVESAHYVA
                     LHLGLWPFPPLSHALWGACIRILNLLPGGAPLFNAFSGICAALALMLIFVLTVRLSYS
                     GLPERYRDPSVLRRSRLIAGFAASAALGLSVPFWFVATRAHVAAFDLMLLLWVVWLLV
                     RFHETRSMKHLLLLSFFWGLAVTEYPTMILLSAAIAPALAVVLWRAKMLNAKTILPAL
                     GCFILSLGVYVVQALLYIRHPAFQWQELTGFFQVLWFIWREQYLALRAGMGANGWIMI
                     LISMLVPALSLIPNTLSGSRTVKYQWTHFLLYSVFVVLAAVLLFNIYPAPWPMVKLRP
                     LLVLPYAVCAVWMGYLAGILYAVVCVRQKDNTRFALALKKSASLVFVPLLAAVLGFAA
                     VLNYAIIDSRPGDRVAKMASGMVRALDSQTWLITDGLLDANLALSAAEQGIPVNLLNV
                     RIAGKKGYLNYAASLFSSPRLQGLARIGIVPLLKEWMQFDDASVEQVAIATDADLWRF
                     MGKDAVPDRGIFKGMSEAMAMDAEEYFSRQQSFWTEARDLFAGEVKPNNMAAFYWYWA
                     RSHLSKVANNSGVHLHDLERPDLAAQAYRAARRIDPDNLSAMLNLHALAGAEKLPEFE
                     ALNRELETFIENMQGKYRLDRLSAQYGMIRDPRLFVQRGLLLAASGQASAALYEMEKV
                     AERSPASTDMQMILAGMYGADQQPDRSAAEYARILEREPENARALSAMVQLSLRRQDP
                     DAAEKYLNTLSTVSNVNPVAVQYQRALVDAARNRMTEAMGRLRDVLRQQPDNLQAGMT
                     LMLIAQAAGDTQTADETMNTLTGRIKSEPRLALSMAVMELQRRNYKEARAYLEGYIRN
                     FRTDLYALDLLLRLDAFERRKGRVEEHVEQILSINPRHAFANYMLGTLQVARGELALA
                     EESYRVSLSAQETPEAMNDLAWLLQSRGAYEEALDLAYRSLAVNDRTPSSWDTYAMVL
                     KGLDRLLEAEEAINMALGLQPNNPVYLYHLALIQEGQGDLKGALQTARPLLDRAAEIP
                     PETFDGIRDLMSRLPQ"
     gene            complement(7894..8241)
                     /locus_tag="PKK36_02275"
     CDS             complement(7894..8241)
                     /locus_tag="PKK36_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93414.1"
                     /translation="MSRYYELRCAVMEAFYSAIVAEQFTVGQAADRCLVEFSSEVQEH
                     GCEALMVLSCLLARTARHDRRALKRFAEEVKALALLEKTPVCWKGLTRREKSRMLEDI
                     RFIRTAMNRPAAS"
     gene            complement(8250..8825)
                     /locus_tag="PKK36_02280"
     CDS             complement(8250..8825)
                     /locus_tag="PKK36_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase domain-containing protein"
                     /protein_id="HNR93415.1"
                     /translation="MRKYSTALAQTLAAAFLFAAAAAGLADGREPVVRRTKDALHAHV
                     QARIEIHRQKLPEEFQHRKNFAWAVAKADGLTKQEYFAHSGIQNLDDLSDEAVMQIRG
                     ISIAPSNAVFETLCVNRRSEVNGPDCWDRKVDTEFKILEDIAAQLGKQTNAAGRIRLY
                     TDLYPCKSCLHVMEQFMEKYPNIALEVLYKD"
     gene            9050..9532
                     /locus_tag="PKK36_02285"
     CDS             9050..9532
                     /locus_tag="PKK36_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93416.1"
                     /translation="MMLVAGIAVGAFVQEQTISAASLAEELPGEEGAVTLQEQEWSGE
                     AALVHKDSPSWSGEEMVRQAIRLGRPLLPAAGEESVSEMDKSPALQISTEFLERLHLN
                     PMKGGAVARSRLEMDPDTGGIGLRKLEMGFEGRGLWLIHTPSTDETEGSMGLELKLRW
                     "
     gene            9754..10368
                     /locus_tag="PKK36_02290"
     CDS             9754..10368
                     /locus_tag="PKK36_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNR93417.1"
                     /translation="MSIREEKKEAKRSAILAAAEGLFRARRYDEVTLDQVAKKARVGK
                     GTIYLYFRNKEALFFQMVTEDFESMLKTVEQIAVSDRDIRRRLLDICSETSAFFTARF
                     MFLQIMHHEEQFLRNRQMQSRLKKDRDRLRAALHRVFLDGIRNGVLRNDCPADVMEIA
                     LVGAMRFRNMSACETGAAIDLEAMIDLFLQGAAACNKKSKRTVL"
     gene            10365..11804
                     /locus_tag="PKK36_02295"
     CDS             10365..11804
                     /locus_tag="PKK36_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNR93418.1"
                     /translation="MKMNIIRISWASLLALLLGGCAVFQPSARNEKAYADSLGEFRLY
                     DEAQVPHITAWWLSFNDPQLNDLMTEALAQNLSLQQARARLRQAWLVAEKQASARRIS
                     AEASAGAGVSEERRETEEGSTNISAENYSLGLAAAYELDLWGRLASLDKAAELDAKAS
                     EADRNIMAGTVASEVALTYFNILWQRETLDIINDQLETARKNLELMELRFRNSQATAL
                     DVLQRRESVAQIEAMIPPARANEESLILQLSILLGKTPTDSVKVKGRRLPPLPPQPDA
                     GLPADLLARRPDVLRAGLRLRSADWTLSAARADRLPSIRLSASAKYASSDTSALFDNW
                     IASLAASLTGPILDGGRRRAEVQRVREVVAERLAAYRETVISAVGEIQEVMIRESGQT
                     EYLKALERQLAASLATREEALERYKKGQENYLTVLSAENGAQQVQRQISLAAHDRLAH
                     RVKLYRALGGDWNAILDETSSTNERKNHE"
     gene            11839..13002
                     /locus_tag="PKK36_02300"
     CDS             11839..13002
                     /locus_tag="PKK36_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR93419.1"
                     /translation="MRATFALLGLAILAAAVFAAVVMIRNKPRAGRREAMRVIPVVEV
                     MNLAAENRQVNIECAGTVIAADEMDVQPLVAGQILKVHPDLVEGGVIQAGEVLAEIDP
                     ADYELQVAQQQAALDKAESALRLEQGQQAVAEREWELLGDEGASRKDRELALRKPQLK
                     AAQAAVDAARASLEQAKLNVARARVTAPANGVVAAVYADAGDRAVPGQPIARIVSTDR
                     YYVRGSVPVNALRWIDVSSGRDASPAAIQLSSGAVREGRVVRLLPDLEAGSRMARILV
                     ELRDPLDMSGNSLDGKVLLGDYVRVTVKGRELKDVLSVPLEVLRDGRELWWLDGDARL
                     HIQPVDIVWMGRSEVYVSNGNPDGWRLIVSDLGTPIEGMELRIAGEGEAGGRP"
     gene            12999..16124
                     /locus_tag="PKK36_02305"
     CDS             12999..16124
                     /locus_tag="PKK36_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR93420.1"
                     /translation="MSSDVMPPEASGPIAWMAHNRVAANLLMMFFFVGGLAAFRFIKQ
                     EVFPDMQENIVTVSVSYPGASPEEVEQGIVLAVEEALSGQEGVDEIQSTASEGHAVIQ
                     ATLLEGVDSIKVYQDLKNEVDRITTLPEDSEEPVTTLEQHRRGVMTLALFGEARETSL
                     RQLGEQVRDALLEDPDITQVDLEGVRPLEISIEIPQENLRRYNLTLQQVANILKTKAL
                     DLPGGGVKTQGGEILVRVVERRDFGHEFADMPIITGPDGSEVRLRDIAAVIDGFEDTD
                     QYGLYNRKPSVQLEVYRVGDQTPVQVGRAVERRLDMIRPGLPDGIEIAVMHSMASVYQ
                     QRVTLLLKNGAIGLVLVLLLLGVFLELRLAFWVMTGIPVSFLGALILMYFWGVSINMM
                     TLFAFIMALGIVVDDAIVVGENVYHHYQEGMPFLRAAVHGAREVGTPVAFSILTNIVA
                     FLPLAVLPGMMGRIMKMLPVVVVCTFVLSWIECLLILPCHLGHHRPRERTGFSKWLHE
                     RQQGFSLRFRQWVQTRYGPFLSRCLTHRYFVVMTACALLIVTLSYVASGRMGFEMFPR
                     IESDYAYGSVVLPYGAHIERTRAAVDRMMDASQKVVDDCGCTQLVKGVIANIGRSGSH
                     TADVRIYLADPEIREKIMSTQQFVERWREAIGEIPGIENMKLMSDRGGPGSGSALTVE
                     LRHRDVDALEKAAADLARELEHFPRVSDIDDGVQRGKQQMDFRIRPESESLGLHAADV
                     ARQIRNAYEGAEVLRQQRGRNEIKVKVRYTENERDSEDDIRNLILRTASGGEVPLEQA
                     VTIQRGRAYTTITHRNGQRTMTVSADVRPRAQAGQVIEALDRETLPALLQRYPGLSYS
                     YEGHQAENRKSGERLSMLIPVTLLAIYALLAVPFRSYMQPLIVMVSIPFGVVGAVAGH
                     LIMGYNMSMMGLIGIVALSGVVINDALILVDFVNRAREKGMPVKQAVVQAGIQRFRPI
                     MLTTLTTFGGLAPMIFETSRQARFMIPMALSLGYGLLFATMITLVLVPSLYMAIEDMR
                     SVFLKKADPAA"
     gene            16124..17506
                     /locus_tag="PKK36_02310"
     CDS             16124..17506
                     /locus_tag="PKK36_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR93421.1"
                     /translation="MRTSVRNINLAEGAVGPLLVRLTIPMIGGMFAMSSFHLADTWFV
                     SRLGVAELAAMTFTFPVVMVVGSVAMGLGIGISSLVSRALGNGRHERVKRLCTDGFLL
                     SLSTVILLALIGLLTVNPLFRMLGATDDEIHLIRQYMVTYYSWVGVLIVPMVGNGIIR
                     ATGDTFWPAVTMVVSALLNIVLDAWLIFGGLGVPPLGLKGAALASALSRAIGMLITFW
                     VLGSRNLFTLHWPGLRTVAGSWAELLYIGVPSALTHMLMPLSGMVTTRLIAMYGVGAV
                     AAWGAADRLMHFSFLIPIALGSALVPFAGQNWGAGRQDRILYGWNRANLFTLAYGAGS
                     MMLAWPLGLPLARLFTHDPAVMRMIAGFLRITLTGSLLIHTAVQGGFLLNGIGMPLRG
                     AMLSTFRLLALQVPLAIAGNRMLGLYGVFWGIAVAQLIAGSAAWAWISAILRSTGHSR
                     LKAKIMGNLG"
     gene            17560..18093
                     /gene="cysC"
                     /locus_tag="PKK36_02315"
     CDS             17560..18093
                     /gene="cysC"
                     /locus_tag="PKK36_02315"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNR93422.1"
                     /translation="MQDRKFKPAVLWFTGLSGSGKSTIAEKLVADLKAAGQELEYLDG
                     DRVREIFPQTGFTREERDLHIRRIGFIASLLEKHGVLVVASFVSPYREARDEVRRRCR
                     HFIEVHVSTPLEECERRDVKGLYARARKGEISHFTGISDPYEPPENPEIELDTASGIS
                     VEEACRRVVQYLKDHCL"
     gene            18090..18995
                     /gene="cysD"
                     /locus_tag="PKK36_02320"
     CDS             18090..18995
                     /gene="cysD"
                     /locus_tag="PKK36_02320"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNR93423.1"
                     /translation="MKTYAMTHLKQLEAESIHIIREVAAEFERPVMLYSVGKDSSVML
                     RLAEKAFHPGKIPFPLLHVDTTYKFPEMITFRDQMAKKIGFELRVHTNQAAIDEGCEP
                     FGYGTQKCCGLLKTQALLDALRAGQYDAAFGGARRDEEKSRAKERVYSFRDSFGQWDP
                     KNQRPELWNIYNAKVNKGESIRVFPLSNWTELDVWQYIYMENIPVVPLYFAKEREIVV
                     REGQLIPIDPRFPIPRGGKVEKVMCRFRTLGCIPCTGAVRSSAKTIPEIIEEMMTIRI
                     SERSTRVIDHDAEGSMEMKKREGYF"
     gene            19006..20937
                     /gene="cysN"
                     /locus_tag="PKK36_02325"
     CDS             19006..20937
                     /gene="cysN"
                     /locus_tag="PKK36_02325"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNR93424.1"
                     /translation="MSTIQEFLAQNEKKELLRFSTCGSIDDGKSTLIGRLLHDSKNVY
                     EDQLASVKNSSREKTSDEKIDFALITDGLKAEREQMITIDVAYRYFSTPKRKFIIADT
                     PGHVQYTRNMATGASTANLAILLIDARNGISDQTRRHAFIVSLLGVPHIVVAVNKMDA
                     VDYSEKVYNEIKEGFEAFSHKLKIVDIRFVPISALKGDNVVSRSARMPWYDGESLLEL
                     LETIYIGSDRNLVDLRFPVQYVIRPDMSYRGYAGTVTSGVIRKGDEVLALPSMKSSRV
                     KSITTFDGEIEEACAPMSVAVTLEDELDISRGEMLVHPHNVPQVSKHLDAMLVWMGDE
                     PLDPGKSYFIKHTTQVGRVRVDQIRYKVDINTLSRTSDMSGLVLNEIGRVALTSFRPL
                     FCDSYTKNRGTGCFVLIDTISNATVAAGMILDREPESQMPVRMTRGKPGAEMHHREGR
                     IALPARMERYGQKPATIWITGLVASGKTDVAYALERKLFDLGGTAVVLDGENVRMGLS
                     SELDFSPEGRAEHLRRVSEMARMLNDAGLLVICAFVSPTAFIREQASEIIGKDRYQEI
                     HLDAPVEWCETRDQSGLYRRARAGDVDNLAGINAPYEKPQHPALRIEADKTGAEPAAD
                     LIVAHLRKTCIFPYVPDRT"
     gene            complement(20947..22167)
                     /locus_tag="PKK36_02330"
     CDS             complement(20947..22167)
                     /locus_tag="PKK36_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93425.1"
                     /translation="MRIRDSFTQLALFLCIVFQFTAGAEYDIRQIADETRINRDPVIS
                     GTGLVAWQSLSGEGTDNAESEVVIYQNGTVKPLTEGSALVAGNLNPRVHGSRAVWTAG
                     SKRAEYVAPQPRVPAGTNAVPADTETAAAAAPEEPAPKKEVKPGRGGDNIVFWDGSEV
                     KVLTNDGRDNAHPSLSDTTIAWQTDRQWPLGWEIMVWNNGEATQVTSNEFYDMAPQVS
                     GNRVVWYGWDGEDYEIFLYDHDTRETTQITDNRYDDIAPVITEDMIAWQGFPSVESDI
                     FVWQDGAIKKLSDNPEDDSHVRASGKLVVWQGFDGDDFEIYLYDGEKVVQLTNNKFDD
                     VEPDVGDGAVCWMGYHNNWDPEIFVLPSIDGVAEMLTSNDYEDRDPKTAGRKVTWRAR
                     NEGRTMVYLAEPKE"
     gene            22344..23168
                     /gene="lpxI"
                     /locus_tag="PKK36_02335"
     CDS             22344..23168
                     /gene="lpxI"
                     /locus_tag="PKK36_02335"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978206.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNR93426.1"
                     /translation="MKDFQTDELGIIAGRGVYPRILAASAKQQGVRRVFAAAFRGETD
                     RRICQVADEVSWVHLGSLGALLDAFKASGVRKAVMAGQIKPTHLFQLRMDCRMIDLLK
                     RLRERNADTIFGALADEMASIGVELLPAYLFMESSMPAPGLLTRKAPSQREQQDIELG
                     LKVAKATSCLEIGQTVVVKEGTVLAVEAFEGTDKAILRAGRLGGRGAVVVKVAKKGHD
                     MRFDIPVIGLRTLKTLRKAKISCVALEAGRTILLEREKVIAEADRMGLSMTAVAMD"
     gene            23180..24130
                     /locus_tag="PKK36_02340"
     CDS             23180..24130
                     /locus_tag="PKK36_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR93427.1"
                     /translation="MPKKTIRVGVVGVGSLGQHHARIYSELPGVELAGVYDASPKTAS
                     AVAARHQTRACDSLEELACNIDAASVAVPTTLHRDVAAVLLKAGVHLLIEKPIAATTA
                     EAEDIVALAQKQDLILQVGHIERFNPVMKFLEEASPKPRFIEAHRLASYPPPREGLHP
                     RGTEVSVILDLMIHDIEIILHLVNSRVKDIHAVGVPVLSPTEDIANVRLLFESGCVAN
                     ITASRISSERMRKIRVFSEDAYISLDYQNQAGKMFRREGGGIVPVEVPIEKGDQLTLE
                     LESFIGCVARHGDPVVSGDHAAEALRLAMRIIAMVQKGSS"
     gene            24127..25254
                     /gene="lpxB"
                     /locus_tag="PKK36_02345"
     CDS             24127..25254
                     /gene="lpxB"
                     /locus_tag="PKK36_02345"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNR93428.1"
                     /translation="MSRSLLVIAGEVSGDMHAAGVVRELQKRDPEMKFWGVGGSRLRE
                     CGMEILYDVKDMAVMGLAEVLRRYGFFKRALDHVLEEAARRRPDAVLLVDYPGFNLRV
                     AEQLKKAGLPVIYYICPQVWAWHRSRIRKIARIVDHLLVIFPFEVDVFKDTGLRVDFV
                     GHPLTDAPEETVSDLPWQGSPRLALLPGSRKQEIHRILPAMLGAVEKLRVEHPNLAVL
                     VACPSDQVAGWVQEFAGPRNNLFRAVTGKTRAVLRTARAALVKSGTSTIEAALAGCPM
                     VLVYKTSWLTYALGRLLVRVPHIGMVNIVAGCPVVPELIQQQATPDTIARAVSPLLSH
                     TSAHDRMKQDLFEVQKLMGKGGASLQAAELILADIENSRKL"
     gene            25328..26689
                     /locus_tag="PKK36_02350"
     CDS             25328..26689
                     /locus_tag="PKK36_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR93429.1"
                     /translation="MARILLVDDEPSILSVLSTLLRAEGYEVVPALGGEKALEELKGK
                     PFDLMISDIRMSPIDGMQLLKAARKDWPKMAVLMITAYGSVETAVGAMKVGAFDYITK
                     PFKVDELLITVQRALEYNNAMAENVTLKSQLEARYQFENIVAESPAMRKVCEMIERVA
                     PTDATVLIQGESGTGKELVAKAIHSYSKRKNGRFVAINCAAMPELLLESEMFGHVKGA
                     FTGASSDKEGLFEVAAGGTLFLDEIGSMPLSIQGKLLRVLQEREIRRVGGTATVPVDV
                     RVLAASNCMLESLIEEGRFREDLYYRLSVIPIEVAPLTQRKEDILPLVYHFLRTEIGP
                     GKDLPEIDSDAQLILENYTWPGNVRELENAIRHAVTFAKGGKITVADLPAKISATVPA
                     TPAAASSPSAQYKGKSLKAFLRTKEKEYLSQVLETAGGDKEAAAKALKISLATLYRKL
                     PED"
     gene            26761..27720
                     /locus_tag="PKK36_02355"
     CDS             26761..27720
                     /locus_tag="PKK36_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNR93430.1"
                     /translation="MVETFEKVRLGISTCLLGQPVRYDGQHKLDTFLRDTLGRFVEYV
                     PVCPEVECGLPIPREAMRLEGNPDNPRLMTHKTRRDITEMQQAWGRKRLKELESEDLD
                     GYIFKSKSPSSGLFRVKVYQESGMPVKQGVGIWARMFTERFPLLPVEEEGRLHDPVLR
                     ENFIERIFVMRRWRKMLAENSTRGGLVEFHTRHKYLILAHSSKAYSDLGKIVAGMKGR
                     NLKEVQSEYLAILMPALTVTATVKKSVNVLQHMQGYFKKQLTADEKKEMSETLNEYAR
                     GFIPLIVPVTLIRHYVRKYGEPYLADQWYLNPHPLELQLRNHA"
     gene            complement(27721..28542)
                     /gene="mutM"
                     /locus_tag="PKK36_02360"
     CDS             complement(27721..28542)
                     /gene="mutM"
                     /locus_tag="PKK36_02360"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HNR93431.1"
                     /translation="MPELPEVETVVLSLRDQGICGRRISSVAVHWMRSVQEPSVEEFT
                     RILKGRTIQNISRRGKFVVMGLSGGWTALVHLRMSGRLFLGRPADITKHDQVLLKLDD
                     GRTLRFHDTRKFGRWRLTQDPETVLSKLGPEPLEPVRDFHKRLHAKSRILKPLLLDQT
                     FIAGLGNIYVDEALWEAKIHPQRKSDSLTTSESDALLKAVRKVLKKGIRNLGTSLGHG
                     KANFQLPDGESGRNREALNVFRQTGLPCPRCGTNIERIKVGQRSTHICPSCQRKH"
     gene            28662..28749
                     /locus_tag="PKK36_02365"
     tRNA            28662..28749
                     /locus_tag="PKK36_02365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28696..28698,aa:Ser,seq:gga)
     gene            complement(28756..>29459)
                     /locus_tag="PKK36_02370"
     CDS             complement(28756..>29459)
                     /locus_tag="PKK36_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNR93432.1"
                     /translation="ILDNGTSQLIIITGPNMAGKSTYIRQVALIVIMAHIGSFVPAGS
                     AEIGLTDRVFTRVGASDDLTRGRSTFMVEMQETANILNNATPKSLIVLDEIGRGTSTF
                     DGISIAWAVAEHLHNHPAVKAKTLFATHYHELTDLALTMAGVKNYNVLVREKGDHIVF
                     LRKIVPGGAAKSFGIQVARLAGLPDQVIERAKEILANLEEGELGETGQPKIAKHGPRK
                     PRASDSQLNLFDPGM"
BASE COUNT         5821 a         8414 c         9020 g         6204 t
ORIGIN      
        1 cggctcctcg gcgatatgct cgaccgccgg agggttgggg agaacatcgc cgctggtgcc
       61 gcgggtcagg acggaggcca ggtcgaaacg gaagccatcc acgcgcatat gaagcaccca
      121 gtagcggagg cagtcgagaa tgaagtcgcg cacgacggga tgattgcagt ttacggtgtt
      181 gccgcagccg gtgtagttgg cgtagtgccg accgttcttt tccatgatgt agtaaatgga
      241 attgtctatg ccgcggaagg agtaggtggg gccgccgtca ccgccctcgg ccgtgtggtt
      301 gaacacaaca tcgaggatga cctcgatccc ggctttgtga agcgctttga ccatttcctg
      361 gaattcgcgg acctgtcctc cgcgtccgcc ggaggccgcg tagcgggcgt tgggcgcaaa
      421 gaaagccagt gtgctgtagc cccagaaatt gcggagcgat ttccggctgg tgttttccag
      481 ataatattcc atctcattga attcctgaat gggcaggaat tcaacagcgg tgatgccgag
      541 atcgcgcaga taaggaattt tctcctcaaa gccgcgatat gtgcccggat atgaaactcc
      601 cgacgaagga tgcacggtga aaccgcgcag gtgggtctcg taaatcacgg actggtgcag
      661 cggggtggac ggatgctgat catcttccca gtcaaaggtg tcgtcgagaa tgacgccgcg
      721 cggaaaggcg gcgccgttca gcggctggtc gccgggcttc aagccatgcg ggtcagccca
      781 cttcggcgcg ccggagacag cgagagcgta gggatccaga agccactgtc tcggattgta
      841 aaagcgcgct tccggtccgt cggccgggcc ctccatgcgg taggcgtaga actgtccggc
      901 ccgggcatcc tctacatgga tatgccagat gtcgccgacc cggttcagct ccggccgcag
      961 ttcaatttcg cgcgaagggc gcgcagcatc cggttcatcg aacagcatga gccatacgcg
     1021 ccgggcatgg cggctgcaga gcgtgaagca gaccccgtca ttcaggacgc tggccccgta
     1081 gggaacgggg aaggccgtgc gcgtatgaat ttttccgtta aatgttcttt tcattttcct
     1141 tcgtatcctt gtgtccgggg acaaaccatg caaaaagttt tttcaccccg cggccggttc
     1201 ctcgaatggt gtgatagctt ccctccgcgg taaaccgtgc cacacctatg gtcccggcca
     1261 cagccggctt cagaagataa tcgccaaccg ctttggccga actgtctaca gcgccggtga
     1321 tgccgtcatt gagccgcttc cagaaatccg gttctccggc cgcctgcggg gccgtcacca
     1381 tgatgcgcgg ccggagcgac ggcagcacgt ccttggtgaa catgtccttc acgtcggtga
     1441 agaaattccc ggcatgggcg gtgatggact ccgccgcccg ggcgcgggtc catccgcggt
     1501 aggctgcgca gcggtccatg gccgcccgac cggccacgga agtcagcagc ccggcgccga
     1561 tgccctgcgc gatatcatcc attacacgac cgacgaacgg gattcccgaa aacagtttgt
     1621 cgaacagggt tttcccgagg ttcagcagat tgacggccgc cacggttctc agcgtgtcgc
     1681 gcagaatcat ccactgctcg cgatggagcg ggcgcgcatt gtagatgcgg gcgatgcgca
     1741 caaccatgct cccgttccgg tacagaaccc cggccgcatc caggctgtga taggggctga
     1801 gtgtgactcc cagcatgaca tcccgcacac tccggcgcac ttcatgggcg gccagcccgt
     1861 tcagcacggc gagcagggga cggatggcct gctcctcggc tgtgcgcgtg tcgcggatca
     1921 ggtcatcgcg cagggggtgg gagcgcagcg tgtcctgcag cagggccatc tgctgctctg
     1981 ccgccgcggc ctgctccggg gagaggctgg ggttgcccga gaggcgccga agatacctga
     2041 tcagatagcg tgtgtagctt gtgagctgcc ggtgagaggc ttcggcaatg ttctccggaa
     2101 gctccggcgg agtcaggacg gccgggaaag acagccactg gtgcaggagc cagaccacga
     2161 gagaaaccga caccactccg acgatgaaaa gatagcccgc cgcggcgcct gcatgaagat
     2221 cgcgcagcag cacataaagc cgcagcagtt caacagcagc catggcaatc agcgcgaagc
     2281 tgatcaccca gacaattcga actgcaatat cccagattcg cctgaacatg attgccctta
     2341 tgaaagcatg tgttcaggtt agcttgtgtg tccgtggcat ctcaagttta aagagatgcg
     2401 cgcaggatcc ggaaggcaat gtctcttccc gtcaggggaa tctcaaagtg ccacgaaaga
     2461 aaacgtccga gcagcccttc gatttcccgg gcctgccggg gctggcaccg cacggcgcga
     2521 gcgccggagg agtggcgggc gcgctgaagg ccgcgcaggg ccgcgaatat gtccggcgca
     2581 aggcgcgcgg gctcttccgg gcaggtgccg gcgcatgtcc tgcagatcag cccgccgcgc
     2641 tcgtgggaaa accatgtgtc gtcccgtccc ggctcgagtt cccgtccgca gtgcagacaa
     2701 tcctgcaggc gtggagccag ccccatgctt tccaggagct tcatttccag ccagaatgtg
     2761 tgctcggccg ccgcgccgcg ggcggccaga tcgtccagtg cttcatccag aaggcgccag
     2821 gtgtccgcat gttcggcgtg caccggtccg attctggctg ccagatccgc gaaataggag
     2881 gccatcgccg cggcccgcca gtcggtgcgc agggccggcc gcattttcag cggacagcat
     2941 tcgctggcga tatgcagttc gcgatgctcc cggccgtaga acagcagttc acaggtgtag
     3001 aacagatcat actggccgag aaaacggctt ttcggccgcc ggccgccttt gatcatggtg
     3061 gacagccggc cccagccggg cgtcagccag gtgacgacgg aggaggtgtt ggacaccggg
     3121 gcgatgcgca gggcgatggc atcggttttc agaatgcgtg acatcagccg cctgttccgc
     3181 ttcgaaggcc caggagaatg agagcaatgc tgtagtagat cagcagcgcg aagatatcgt
     3241 tggatgaagt cacaaacggg cccgaagcga cggcaggatc caccctgaga cggttgagcg
     3301 caatgggcac cattgcgccg aaaacggccg cgaaggtcat ggcggaaaaa agggccaccc
     3361 ccaccgtaag cgaaaggaaa agcggtgtgt gcaggtcgtt ctcgtgaccg gacaggtgga
     3421 tcagatagcg ggcccacagg ccgataatgg ccgcgcaggc aatccccatg atggcccccg
     3481 ctgagatttc gcgccccagc accttgagca tgctgcggcc ctccagcgtg cccagcgcca
     3541 gtccgcggac gaccagcgtg gaagactgga tcccggtgtt tcctcccatg gccataacca
     3601 caggcacgaa gaagctgagc accatgacct gggaaatatc attcagaaac ttcttgaaaa
     3661 tgaaactcgt cacaaacccc gtggccagcg tgatcagaag ccatggaagc cgcgccccgc
     3721 atgcgtgcag cggggtcatg gaggtgagtt cggagtcatc cgacccggcg aagcggaaga
     3781 tgtcctcgga cgcctcttcc tgcagcacgt ccaccacatc gtccaccgtg acccgcccaa
     3841 gcagctgtcc gtcggcatcg gtgaccggca cggccagcag gtcatagcgt gaaatgatgc
     3901 gggccacctc ttcctgatcc gtgtccgctt tcacggatac gacatccttg tgggcgatgg
     3961 ctccgagggt gatctgccgg ttgcggattt tcaggagctc ccacagcccg ataaagcctt
     4021 tcagtttctg gtcgtcatcc acgacatacg cataataaaa cgcctcttct ccctccatga
     4081 atgcaatggc atcgagagct tcagctacag tcatattctc acgaaccgcg accacctcgg
     4141 gagtcatgat tccgccggcg gtgttttcgt cgtacttcag cagattgcgg acgtcgcggg
     4201 actcctcttc ctccatgagt tcgagcactt cctcggaacg ctcttcggga agttcggcga
     4261 tcacgtcagc cgcatcgtcg gggtccattt cctcgacgag ttcggcaagc tcattgttgc
     4321 tgagagattc aagaacctcg tcttcagcaa ggccttccag ttcggccagc acatcgggtt
     4381 tgaattcttc gtcaatgagg gcaaaaactc tgacacgctc ttccgggagc agtttctgga
     4441 gcagttcggc cagatccggg gcgcgccggg aatcgagcat ttccgcaagc tggttccact
     4501 gctcttcgcg gatgagcctg cgaacctccc gtttcagctg tttcatcgtc agcatttgcg
     4561 tccaatctgt gagataaaac agcattctac tcttacgcgg gacggactcg caacagcgtt
     4621 tttccgtcag tcgaaaaaaa cctgcgtcct ctgagatgaa tattcggctt ttctgctttt
     4681 atgaatccgc ggaatgctgt actttgcgaa actggattgt cgaaaggttg tggagaaaac
     4741 agacaacgtg agcacaagca aatcagagat tcgcgcgcgc tggaaaccgg gggcggccgt
     4801 tctgggggcc gcgctggcgg tttattattt aaccctcagc cgggtctttt atccggttga
     4861 atctgcccat tatgtggcgc tccatctggg cttgtggcca ttccctccgc tgtctcatgc
     4921 gctgtggggg gcctgcatca gaatcctgaa cctgctgccc ggcggcgcac cgcttttcaa
     4981 tgcattcagc ggcatatgcg ccgcgctggc cctgatgctc atttttgtgc tgaccgtgcg
     5041 tctgagctac agcggtttgc ccgaaagata ccgcgatccc tccgtgctgc gccgttcccg
     5101 cctgatcgcg gggttcgcgg catcagccgc gcttggactg tccgtccctt tctggttcgt
     5161 ggcgacaagg gcccatgtgg cagcgtttga cctgatgctt ctcttgtggg tggtctggct
     5221 gctggtgcgg tttcacgaaa cccgttcaat gaagcacctg ctgctgctgt cgttcttctg
     5281 gggtcttgcg gtcaccgaat atccgaccat gatcctgctg agcgcagcca ttgctccggc
     5341 gctggccgtg gtgctctggc gcgcaaagat gctcaatgcg aaaaccatcc tgccggcgct
     5401 gggctgcttt atcctgagcc tgggcgttta tgttgtccag gctctgcttt atatccgcca
     5461 ccccgcgttc cagtggcagg aactgacagg tttctttcag gtgctctggt tcatctggcg
     5521 cgaacagtat ctggccctgc gggcaggcat gggtgcaaat ggatggatca tgatactgat
     5581 cagcatgctg gtgccggccc tgtccctgat ccccaacaca ctgtccggtt cgcgcacggt
     5641 aaagtatcag tggacgcatt tcctgctcta ttccgtgttt gtggtgctgg cggcggtcct
     5701 cctgttcaat atatatccgg ctccctggcc gatggtcaaa ctgcggccgc tgctggtgct
     5761 tccctatgcc gtatgcgccg tatggatggg ttatcttgcc ggtattctgt atgcggtggt
     5821 ctgtgtccgg cagaaggaca acacccgttt tgcccttgcc ctcaagaaat ccgccagcct
     5881 tgtgtttgtt cctctgcttg ccgcagtcct tggctttgcg gcggttttga attatgcgat
     5941 tattgactcc cgtcccggag accgtgtggc gaaaatggcc tccggcatgg tgcgcgcact
     6001 ggattcccag acatggctga tcaccgatgg actgctggat gcaaacctcg ccctgtcagc
     6061 cgcagagcag gggatacccg tcaatctgct caatgtccgc attgcgggca aaaagggtta
     6121 tctgaactat gccgcttcac tgttttcttc cccccgcctg cagggactgg cccgtattgg
     6181 cattgttccg cttctgaagg agtggatgca gtttgatgat gcatccgttg aacaggtggc
     6241 catcgccacg gatgcagatc tctggcggtt catgggcaag gatgcggtgc ctgaccgggg
     6301 catcttcaag ggcatgtccg aggcgatggc catggatgca gaggagtatt tcagccggca
     6361 gcagtcattc tggaccgagg cgcgcgatct gttcgcggga gaagtgaagc cgaacaacat
     6421 ggccgccttc tactggtact gggcccggag ccatctttcc aaggtggcga acaacagcgg
     6481 agtgcatctg catgatctgg agcgccccga tcttgccgcg caggcgtacc gcgcggcgcg
     6541 ccggattgat ccggacaatc tcagcgccat gctgaacctg cacgcgctgg ccggagcgga
     6601 gaaactcccg gagtttgagg cgctgaacag ggaactcgag acgtttattg agaacatgca
     6661 ggggaaatac cggctggacc ggctgtccgc acagtatgga atgattcgcg atccgcgcct
     6721 gtttgtgcag cgcggcctgc tgctggcggc cagcggtcag gccagcgccg cgctgtatga
     6781 gatggaaaag gtggctgagc gttcccctgc cagcaccgat atgcagatga ttctcgccgg
     6841 catgtacggc gctgatcagc agcctgaccg gagtgccgcc gagtatgccc gtattcttga
     6901 gcgcgagcct gaaaatgcaa gggcgctgtc ggccatggtg cagctcagtc tgcgccggca
     6961 ggatcccgac gcggcggaga agtacctgaa caccctcagc acggtctcca atgtgaatcc
     7021 ggttgctgtt cagtaccagc gggcgctggt ggatgccgcc cgcaaccgga tgacggaggc
     7081 catgggccgg ctcagggatg tgctccgcca gcagccggac aacctgcagg cgggcatgac
     7141 actcatgctg atcgcgcagg ccgcgggcga cacccagacc gcggacgaaa ccatgaatac
     7201 cctgacgggc cggattaaat ccgagccgcg cctggctctc agcatggccg ttatggaact
     7261 tcagcgccgc aattacaaag aggcccgcgc ctatctggaa ggttacatcc ggaatttccg
     7321 cacggatctt tatgcgctgg atctgcttct ccgcctcgat gcatttgagc gcagaaaggg
     7381 ccgggttgaa gagcatgtgg agcagatcct gtccatcaat ccacggcatg cattcgcgaa
     7441 ttatatgctc ggcacgctgc aggtggcccg cggcgaactc gcgctggcgg aggaatccta
     7501 ccgggtgagc ctttctgcgc aggagacgcc ggaagccatg aacgaccttg catggcttct
     7561 gcagtcccgc ggcgcctatg aggaggctct tgacctggcc taccgctcac tggctgttaa
     7621 tgacagaact ccatccagct gggataccta tgcgatggtt ctcaaggggc tggatcgtct
     7681 tctggaggct gaggaagcca taaacatggc gctgggcctg cagccgaaca atcctgtata
     7741 cctctaccat ctggcgctga ttcaggaggg acagggagac ttgaaggggg ctctgcagac
     7801 agctcgtccg ctcctggatc gggcggcgga gattcctccg gaaacctttg acgggattcg
     7861 cgatctgatg tcccgcctgc cgcaatagga gcgtcacgag gcagcgggcc ggttcatcgc
     7921 ggtccggatg aatcgaatat cctccagcat ccgcgacttt tctctgcgag tcaggccttt
     7981 ccagcagacc ggcgtttttt ccagcagtgc gagtgctttg acttcctcgg caaatcgctt
     8041 cagcgccctg cggtcatggc gggcggtccg cgccagaaga cagctcagca ccatcagggc
     8101 ctcgcacccg tgctcctgca cttcagagct gaattccacc aggcagcggt cagctgcctg
     8161 acccaccgtg aattgctcgg ccacgatggc cgagtagaat gcttccatca cggcacaacg
     8221 cagttcataa tatctgctca tgatgcgcgt cagtccttat acagcacttc cagcgcgatg
     8281 ttcggatatt tttccatgaa ctgttccatc acatgcaaac agctcttgca gggatataaa
     8341 tcggtataaa gccggatccg gcctgctgcg ttggtttgct ttccgagctg cgcggcaatg
     8401 tcctcaagaa tcttgaattc agtgtccact ttccgatccc agcagtccgg cccgttcact
     8461 tcacttctcc ggttcacgca aagagtttca aaaacggcat tggaaggcgc gatggaaata
     8521 ccgcgaatct gcatgacggc ctcgtccgac aggtcatcca gattctgaat gccgctgtga
     8581 gcaaagtact cctgttttgt cagtccatcc gccttggcga cggcccatgc gaaattcttg
     8641 cggtgctgga attcctccgg tagtttttgg cggtgaattt cgatgcgcgc ttgcacatgg
     8701 gcatgaagcg catctttggt ccggcggacc accggttccc tcccatccgc caaccctgcc
     8761 gccgcggcgg cgaaaaggaa agccgctgca agtgtttgtg caagagctgt tgaatatttt
     8821 ctcattccgt ctccagtctt tgggtcccgg cgcgaagggg ttcttctata aaccgcaccg
     8881 cagtccattc cgggcaattg acaaaacagt ttagtacagc ttctgtgcag tctcaagaaa
     8941 ttgttgcgtg caggagtgga cagaaccatg ctgccggtct tcgaaaacgc ttttcggcat
     9001 gcgcagaaga tattatactc tgcgtatgtg ggaaaagctt tctgcgaaga tgatgcttgt
     9061 ggccggaatt gcggtcggag ctttcgttca ggagcaaacg atatccgcgg cttcgcttgc
     9121 ggaagagctt ccgggcgaag agggggccgt cacattgcag gagcaggaat ggtccggcga
     9181 agccgcgcta gttcacaaag acagcccgtc ctggagcggg gaggagatgg tgcggcaggc
     9241 gatacggctg ggccggccgc tgctgccggc ggcaggagag gaaagtgttt ctgaaatgga
     9301 taaaagccct gccctgcaga tcagcacgga atttcttgag cgccttcatc tgaatccgat
     9361 gaagggcggg gctgtggccc gctcaagact ggaaatggat ccggacaccg gcggcatcgg
     9421 actgcggaag ctggagatgg gttttgaggg tcgcggcctc tggctgattc acactccctc
     9481 cacggacgag accgaaggct ccatgggcct ggagctgaaa cttcgctggt gactccccaa
     9541 tcatgccggg ttaggaacgg ctgaggaaga aacgggtaga gcaaggcgtc cattaccgga
     9601 accgacagag cggttccctc cacaggttta tggagggtcg cgctccttcg cgaccggatc
     9661 gtgatcagaa ggtttcgacg aagcacagcc catcaaacaa aagataccgg ttctgcttga
     9721 caaactgact gactggtcag tattttgtgg cgcatgagta tccgggaaga gaagaaggaa
     9781 gccaagcggt cagcgattct tgcggcagcc gaaggcctgt tccgcgcccg cagatatgac
     9841 gaggtcacac tggaccaggt ggcgaaaaag gcgcgcgtcg gcaagggaac gatctatctc
     9901 tatttcagaa acaaagaagc tctgttcttc cagatggtca ccgaggattt tgaaagcatg
     9961 ctcaaaacgg ttgagcagat tgccgtttct gaccgcgaca tccgccgccg cctgctcgac
    10021 atctgttctg aaaccagcgc ctttttcact gccagattca tgtttctcca gatcatgcat
    10081 catgaagagc agtttctgag aaaccggcag atgcagtcgc gcctgaagaa ggatcgcgac
    10141 cgtctgcgtg cggcccttca cagggtgttt cttgatggga tccgcaatgg ggtgctccgc
    10201 aatgattgcc cggctgatgt gatggaaatc gctttggtgg gtgccatgcg cttccgcaat
    10261 atgtccgcgt gcgagacggg ggcagctatt gaccttgagg cgatgattga tcttttcctt
    10321 cagggcgctg ccgcctgcaa taaaaagagt aaacggaccg ttttatgaaa atgaatatca
    10381 tcagaatttc ctgggcatcc ctgttggcgc tgctgcttgg gggctgcgcc gttttccagc
    10441 cgtcggctcg gaatgaaaag gcctatgcgg attcactggg cgagttcagg ctttatgacg
    10501 aggcccaggt gccgcacatt acggcatggt ggctgagttt caacgatccg cagctcaacg
    10561 acctgatgac agaagcgctg gcccagaacc tgtcgctgca gcaggcccgg gctcgactgc
    10621 gccaggcgtg gctggttgcg gagaagcagg cttctgcccg aagaatttcc gccgaggctt
    10681 ccgccggagc gggggtttcg gaagagcgtc gcgagaccga agagggctca acaaatattt
    10741 cggcggagaa ttattcactg ggcctcgcgg cggcctatga actggatctc tgggggcgtc
    10801 tggcctcgct ggacaaggca gcggaactgg atgcgaaagc cagtgaagcg gaccgcaaca
    10861 tcatggccgg cacggtggcg tccgaggtcg cgctgaccta tttcaacatc ctttggcagc
    10921 gcgaaacgct ggatattatc aacgatcagc ttgagactgc gcgaaaaaat ctggaactga
    10981 tggaactgcg cttccgcaac tcgcaggcga ccgcgctgga tgtgctgcag cgccgcgaat
    11041 ccgttgcgca gatcgaggcc atgattccgc cggcccgggc gaacgaggag tcgctgatcc
    11101 ttcagctttc catccttctg gggaaaacgc cgaccgactc tgtgaaagtc aagggtcgaa
    11161 ggctgccgcc gctgccgcct cagccggatg ccggtctgcc ggcggacctg ctggcccggc
    11221 gtccggatgt gctgcgcgcg ggtctccgtt tgcggtcagc cgactggacc ctttcggctg
    11281 cgcgcgccga ccggctgccc tcgatccgtt tgtcggcttc cgccaaatac gcgagcagtg
    11341 acacttcggc tctctttgac aactggattg catcgctggc ggcgtcgctg accggcccta
    11401 ttctggacgg cggccgccgt cgcgcggaag tgcagcgggt gcgcgaggtg gtggccgaac
    11461 ggcttgcggc ctacagggaa acggtgatca gcgccgtagg cgaaatccag gaagttatga
    11521 ttcgtgagag cggacagacg gaatatctga aggcgctcga acgtcagctc gcggcttcgc
    11581 ttgcgacacg ggaggaggcg cttgagcgtt ataagaaggg gcaggagaat tatctgactg
    11641 tcttgagcgc ggaaaacggc gcgcagcagg tccagcggca gatatcactg gcggcgcatg
    11701 atcgtctggc ccaccgcgtg aaactctatc gtgcgctggg cggcgactgg aatgccattc
    11761 tggatgaaac atcttcgaca aatgagagga aaaaccatga atgaccggat ggattcaaaa
    11821 ggcaaaagca aatcacgcat gcgagcgaca ttcgccctgc tggggttggc catccttgcg
    11881 gcggcggttt ttgcggccgt tgtcatgatc aggaacaagc ctcgcgcggg caggcgcgag
    11941 gccatgcgcg tgatcccggt ggtggaggtg atgaatttgg ccgcggaaaa ccgtcaggtg
    12001 aacattgaat gcgccgggac ggtgattgcc gccgatgaaa tggatgtaca gccgctggtg
    12061 gccgggcaga tcctgaaggt tcatcccgat ctggtggagg gcggcgtgat tcaggcgggc
    12121 gaagtgctgg cggaaattga tccggcggat tacgagcttc aggtggctca acagcaggct
    12181 gcactggaca aggcggaaag cgctctgcgc ctggagcagg ggcagcaggc ggttgccgaa
    12241 cgcgagtggg aacttctggg cgatgaaggc gccagtcgca aggaccgcga actggcgctc
    12301 cgtaaacccc agctcaaggc cgcccaggcc gcggtggatg cggccagagc atcgcttgaa
    12361 caggcgaagt tgaatgtggc tcgcgcccgt gtgacggcac ccgccaacgg cgtggtggcg
    12421 gcggtgtatg ccgatgcagg cgaccgcgct gttcctggtc agcccatagc ccgcattgtg
    12481 agcaccgacc gctattatgt ccggggttcc gttcccgtca atgccctgcg gtggattgat
    12541 gtttcctccg gccgggacgc ctcgcctgcc gcaattcaac tttcatcggg agccgtaagg
    12601 gaaggccgtg tggttcgtct gctgccggat cttgaagcgg gcagccgcat ggcaagaatt
    12661 ctggtggagt tgcgcgatcc ccttgacatg tccgggaatt cattggatgg caaggttctg
    12721 ctcggggatt atgtccgtgt cacggtgaag ggtcgcgaat tgaaagatgt cctcagtgtc
    12781 cctctggaag ttctgcgcga cggccgtgag ctctggtggc tggatgggga tgcgcgcctg
    12841 cacatacagc ctgtggatat tgtctggatg ggccggtctg aagtgtatgt ctcaaatggg
    12901 aatccggatg gctggcgcct gatagtttct gatctgggca cccccatcga gggcatggaa
    12961 ctgcgcatcg cgggcgaggg ggaagccgga ggccgtccat gagcagtgat gtcatgcctc
    13021 cggaggcaag cggacccatc gcatggatgg cccacaaccg ggttgcggcc aacctgctga
    13081 tgatgttttt cttcgtcggg ggtctggcgg ccttccggtt catcaagcag gaggtttttc
    13141 ctgacatgca ggagaacatt gtcacggttt cagtctcgta tccgggagcc agtccggagg
    13201 aagtggaaca gggcatcgtg cttgcggtcg aagaggcctt gagcgggcag gagggagtag
    13261 acgagatcca gtccacggcc agcgaaggcc atgccgtcat tcaggccacg ctgctggagg
    13321 gtgtggattc catcaaggta tatcaggatc ttaaaaacga ggtggaccgg atcacaactc
    13381 ttccggaaga ctccgaggag cctgtcacca cgctggagca gcaccggcgc ggggttatga
    13441 cgctggctct gttcggcgag gcccgggaga cgtcgctgcg gcagcttggg gagcaggtga
    13501 gggatgcgct gctggaggat ccggatatca cgcaggttga tctcgaaggc gttcgtccac
    13561 tcgaaatcag cattgagatt ccacaggaga atctccggcg ctacaatctg acgctccagc
    13621 aggtggcgaa tattctcaag accaaggcgc tggatctgcc gggcggcggg gtcaagacac
    13681 agggcggcga aatccttgtg cgtgtggtgg agcgccgcga tttcgggcac gagttcgccg
    13741 acatgccgat cattaccggt cccgacggct cagaagtccg tctgagggac attgccgcag
    13801 taattgatgg attcgaggat actgaccagt acggccttta taacagaaag ccctccgtgc
    13861 agcttgaggt ctatcgcgtc ggggatcaga ctccggtgca ggtgggcaga gcggttgagc
    13921 gtcggctcga tatgatccgc cccggccttc cggacggaat cgagatcgcc gttatgcaca
    13981 gcatggccag tgtttatcag cagcgcgtca cactgctgct caagaacggg gccatcggac
    14041 tggtgctggt tctcctgctt ctgggcgtgt tcctggaact gcgactcgcg ttctgggtca
    14101 tgacggggat tcccgtttcc tttcttggtg cgctcatcct tatgtacttc tggggcgttt
    14161 ctatcaacat gatgaccctg ttcgctttca ttatggcgct gggcattgtg gtggatgacg
    14221 ccatcgtggt gggcgagaat gtctatcatc actatcagga gggcatgcct ttcctgcggg
    14281 cggcggtgca cggcgcccgc gaggttggca ctcctgttgc cttcagcatt ctgacaaaca
    14341 tagtggcctt tctcccgctt gccgtgctgc ccggcatgat ggggcgcatc atgaagatgc
    14401 ttccagtcgt ggtggtttgc acctttgtgc tgtcatggat tgaatgtctg ctgatattgc
    14461 catgtcatct cggccatcac cgtccgcgcg agcggacagg cttcagcaaa tggctccacg
    14521 agcgccagca ggggttcagc ctgcggttcc gccaatgggt tcagacccgg tatggcccgt
    14581 ttctctcccg ctgcctcacg caccggtatt ttgttgtcat gacggcgtgc gcgctgctga
    14641 ttgtgacgct gtcttatgtg gccagcggcc gcatgggttt tgaaatgttc ccgcgcatag
    14701 agagcgatta tgcgtatggc tccgtggtgc tgccttatgg cgcgcacata gagcggaccc
    14761 gcgcggcagt tgaccgcatg atggatgcct cgcaaaaggt tgtggacgac tgcggctgca
    14821 cacagctggt caagggggtc attgccaata tcggccgcag cggatcacat acagcggatg
    14881 tgcggatcta tctggctgat ccggaaatcc gggagaaaat catgagcacg cagcagtttg
    14941 tggagcgctg gcgcgaggcc atcggcgaaa tacccgggat tgaaaacatg aagctgatgt
    15001 cggaccgcgg agggcccgga agcggttcgg ccttgaccgt ggaattgcga caccgggatg
    15061 tcgatgcgct ggagaaagcg gcggccgatc tggcgcggga actggagcat ttcccgcgtg
    15121 tgtctgacat tgatgacggt gtgcagcgag gcaagcagca gatggatttc cgtattcgac
    15181 cggagagcga aagccttggc cttcacgccg cggatgttgc ccgccagata cgaaatgcct
    15241 atgagggggc cgaggttctc cgccagcagc gaggacgcaa tgaaatcaag gttaaagtcc
    15301 gctatacgga aaacgagcgc gattcagagg atgatattcg gaaccttatc ctgcggacag
    15361 cctctggcgg agaggtccct cttgagcagg ctgttaccat tcagcgcggc cgagcctaca
    15421 cgaccatcac tcacaggaac gggcagcgca ccatgacggt atccgcggat gtccgtccgc
    15481 gagcgcaggc cggccaggtg atcgaagccc tcgatcgcga gacactgccg gccctgctgc
    15541 agcgttatcc gggtctgagc tacagctatg aaggccacca ggcggagaac cgcaagagcg
    15601 gcgaaaggct ttccatgctg attcccgtca ccctgctggc aatctacgcc ctcctggccg
    15661 tgccgttccg cagctacatg cagcccctca ttgtaatggt cagcattccc tttggggtgg
    15721 tgggagcggt ggccggtcac ctgatcatgg gatacaacat gagcatgatg ggcctgatcg
    15781 gaattgtggc cctgtcaggc gtggtgatca acgatgccct gattcttgtc gacttcgtga
    15841 accgtgcaag ggagaaaggg atgccggtga agcaggcagt ggttcaggcc ggcattcagc
    15901 gttttcgccc gattatgctg accacgctga ccaccttcgg cggactggcg ccgatgatct
    15961 ttgagacttc aagacaggcc cgcttcatga ttcccatggc tctttcgctg ggttatggac
    16021 tgctctttgc tacaatgatc acacttgttc tggttccctc cctgtacatg gcgattgagg
    16081 atatgcggag cgtgttcctg aagaaggcag atccggccgc ttgatgcgca cgtcggtcag
    16141 gaacataaac cttgccgagg gggctgtcgg gccgctgctc gtccggctga ccatcccgat
    16201 gatcggcgga atgttcgcca tgtcttcgtt ccacctggcc gacacatggt ttgtgtcgcg
    16261 gctgggggtt gcggagctgg cggccatgac tttcacattc cccgtggtca tggtggtggg
    16321 gtccgtagcc atgggtctgg gcattggcat ttcttccctt gtctcccgcg cacttggaaa
    16381 tggacgccat gaacgcgtga agaggctttg cacggacggc tttctcctct cactctcgac
    16441 ggtcattctc ctggcgctga ttggtctgct gacagtcaat ccgctgttcc gtatgctggg
    16501 cgcaacagac gacgaaatac acctgatccg gcagtatatg gtgacatact acagctgggt
    16561 cggcgttctc attgttccca tggtgggcaa cggaatcatt cgtgccacgg gagatacttt
    16621 ctggccggcg gtcacgatgg tcgtcagcgc tctcttgaac attgttcttg atgcatggct
    16681 gattttcggc gggctgggtg ttcctccgct gggcttgaag ggggccgccc tcgcatcggc
    16741 tttatcgcgg gccatcggca tgctcatcac gttctgggtt ctcggctcgc gcaatctctt
    16801 cacgctgcac tggcccggtc tgcggacggt ggccggctcc tgggcggaac ttctttacat
    16861 tggggtgcct tccgccctga cacacatgct gatgcccctt tctggaatgg tcaccacccg
    16921 gctgattgcc atgtatggag tgggcgctgt cgcggcctgg ggcgccgccg accggttgat
    16981 gcatttctcc tttctcattc cgattgcgct gggcagcgcc ctggttcctt ttgccggcca
    17041 gaactggggt gccggtcggc aggaccgtat cctgtacggg tggaaccggg ccaatctgtt
    17101 tacactggct tatggcgcgg gcagcatgat gctggcatgg ccgctcggcc ttcctctggc
    17161 tcgactgttc acgcatgatc cggcggttat gcggatgatt gccggatttc tccggataac
    17221 gctgaccggt tcgcttctca ttcacaccgc cgtgcagggg ggcttcctgc tgaacggcat
    17281 cggcatgcct ctgcgtggtg cgatgctttc cacgttccgc ctgctcgcgc tgcaggtgcc
    17341 gctggccatc gcgggaaacc ggatgctggg cctctatggt gtcttctggg gcattgcggt
    17401 tgcgcagctc attgcgggat ctgccgcatg ggcgtggata tcggccatcc tgcggtctac
    17461 cgggcattcc cgtctcaaag caaagatcat gggaaatctg ggttgaactg tggcgccgcc
    17521 ggtggcatgc tgtctgcctg tttttgaggc ggatgaataa tgcaggacag gaaattcaaa
    17581 ccggcggttt tatggttcac cgggctttcg ggctccggca agagcaccat agccgagaag
    17641 ctcgttgcgg atcttaaggc ggccgggcag gaactcgagt atctcgacgg agaccgcgtg
    17701 cgcgagattt tcccccagac cggcttcacg cgcgaggagc gcgatctgca catccggcgc
    17761 atcggtttca ttgcctcact tcttgaaaag cacggagtgc tggtggtcgc atcgttcgtt
    17821 tcgccctatc gcgaggctcg cgacgaggtt cgccgccgct gccggcattt cattgaagtt
    17881 catgtttcca ctccgctgga ggagtgcgag cgccgcgatg tgaaggggct ttatgcccgc
    17941 gcgcgaaaag gggaaatcag ccattttaca gggatcagcg atccctacga gccgcccgaa
    18001 aaccccgaaa tcgagctgga cacggcctct ggaatttccg tggaggaggc ctgccggcgg
    18061 gtggtgcagt acttgaagga tcactgtcta tgaaaacgta tgcgatgaca catttgaagc
    18121 agctggaggc ggagagcatt cacatcattc gcgaagtggc cgcggagttt gaacgtccgg
    18181 tcatgcttta ctcggtgggc aaggattcct ctgtcatgct tcgcctggcc gagaaggcct
    18241 ttcaccccgg caagatacct ttcccgctgc ttcatgtgga tacgacctac aagttccccg
    18301 aaatgatcac cttccgcgac cagatggcaa agaaaatcgg gtttgagctt cgtgtgcaca
    18361 ccaatcaggc ggccattgac gagggctgcg agccttttgg ctacggcaca cagaaatgct
    18421 gcggcctgct caagacgcag gcgctgctgg atgcactgcg ggccggccag tatgacgcgg
    18481 ccttcggagg cgcccgccgt gacgaggaga aatcccgcgc caaggagcgt gtgtattcct
    18541 tccgtgacag cttcgggcag tgggatccga aaaaccagcg gcccgaactc tggaatatct
    18601 acaacgcgaa agtcaacaaa ggcgaatcta tccgggtttt cccgctgtcc aactggactg
    18661 aactggatgt ctggcagtac atctatatgg agaatattcc cgttgtgccc ctgtattttg
    18721 caaaagagcg cgagattgtc gtacgcgagg ggcagttgat ccccattgat ccccgtttcc
    18781 cgatcccgcg ggggggcaag gtggagaagg tgatgtgccg gttccgcacg ctgggctgca
    18841 tcccctgcac cggagcagtc cgctcttcgg caaaaacaat tcccgaaatc attgaagaga
    18901 tgatgaccat tcgcatttcc gagcggtcca cgcgcgtgat tgaccatgat gccgagggct
    18961 ccatggaaat gaagaaacgc gaaggctatt tttgaggagc acggcatgag caccattcag
    19021 gaatttctcg cgcaaaacga aaaaaaagag ctgctccgct tctccacctg cggcagcatt
    19081 gatgatggaa aatccacgct gatcggacgc ctgcttcatg actcgaagaa tgtctatgaa
    19141 gatcagctgg cctctgtgaa gaacagctcg cgtgaaaaga catcggatga aaaaatcgat
    19201 tttgccctga tcaccgacgg cctcaaggcc gaacgcgagc agatgatcac cattgatgtg
    19261 gcctaccgct atttttccac cccgaagcgg aagtttatca ttgccgacac cccggggcac
    19321 gtgcagtaca cgcgcaacat ggccaccggc gcatccacgg ccaacctcgc catcctgctg
    19381 attgacgccc gcaacggaat cagtgaccag acccgtcgcc atgccttcat tgtttccctg
    19441 ctgggcgtgc cgcacattgt ggtggccgtg aacaagatgg atgccgtgga ctacagcgag
    19501 aaggtgtaca atgaaatcaa ggagggattc gaggcgttct cccacaagct taaaattgtg
    19561 gatatacgct tcgtccccat cagcgccctg aagggcgaca atgtggtgtc acgcagcgct
    19621 cgcatgccct ggtatgacgg ggagagtctg ctggagctgc tggagacgat ctacattgga
    19681 agcgaccgga atctcgtgga tctccgcttc cccgtgcagt atgtcatccg gccggacatg
    19741 agctaccgcg gctatgccgg aacggtcact tccggtgtaa ttcgcaaggg cgatgaggtc
    19801 ctggcgctgc cctcgatgaa gagcagtcgc gtgaaatcca ttaccacttt cgatggcgaa
    19861 atcgaagaag cctgtgcccc catgtcggtt gcagtgacgc tggaggacga gctggacatc
    19921 agtcgcggcg agatgctggt ccatccgcac aatgtcccgc aggtgtcgaa gcatctggat
    19981 gcgatgctgg tgtggatggg cgatgagccg ctggatccgg ggaagtccta tttcatcaag
    20041 cacaccacgc aggtgggccg ggtccgcgtg gaccagatac gctacaaggt ggacatcaac
    20101 accctcagcc gcaccagcga tatgtccggc cttgtgctca atgagattgg ccgcgtggcc
    20161 ctgacctcat tccgtccgct gttctgcgat tcctacacca agaatcgcgg taccggctgc
    20221 ttcgttctga ttgacacgat ctccaacgcc accgttgcgg cgggtatgat tctggatcgc
    20281 gagccggaaa gccagatgcc ggtgcggatg acacgcggca aacccggcgc tgaaatgcac
    20341 caccgcgagg gccgcattgc gctgcctgcc cgcatggagc gctacggcca gaaacccgcc
    20401 accatatgga tcacggggct ggtggcctcc gggaagacgg atgtggccta tgccctggag
    20461 cgcaaattgt ttgacctcgg gggaacagcc gttgttctcg atggcgagaa tgtccgcatg
    20521 ggcctcagca gcgaactgga tttttctccg gagggccggg ctgaacatct gcgccgggtt
    20581 tcggaaatgg cgcggatgct gaacgatgcc ggactgcttg tcatctgcgc gttcgtttct
    20641 ccgaccgctt tcattcgtga acaggcgtcg gaaatcatcg gcaaagaccg ttatcaggaa
    20701 atccatctgg atgcgcccgt ggaatggtgt gaaacccgcg accagtctgg actgtaccgc
    20761 agggcccggg caggggatgt ggacaacctg gcggggatca atgcgcctta tgaaaagccg
    20821 cagcatcctg cgcttcgcat tgaagccgac aagaccggcg cggaaccggc ggcggatctc
    20881 attgttgcgc atctgcgcaa gacctgcatt ttcccgtatg ttcccgaccg gacgtgagcc
    20941 gcgggtttat tctttcggtt cggccagata aaccatcgtc cggccttcgt tgcgcgcgcg
    21001 ccaggtgact ttccgcccgg cggttttggg gtcgcgatcc tcgtagtcgt tgctggtgag
    21061 catttcagcc actccgtcaa tcgaaggcag tacaaaaatt tccgggtccc agttgttgtg
    21121 atagcccatc cagcagaccg cgccgtcccc gacatccggc tcgacatcgt cgaatttgtt
    21181 gttggtgagc tggaccacct tttctccgtc gtacagatag atttcaaagt cgtccccgtc
    21241 aaacccctgc cacaccacaa gctttcccga tgcgcggaca tgagagtcat cttccggatt
    21301 gtcactcagt ttcttgatgg ccccgtcctg ccagacgaaa atgtcgctct ccacagaggg
    21361 gaatccctgc catgcgatca tatcctctgt gatcacaggg gcaatgtcat cataacggtt
    21421 gtcggtgatc tgagtggttt cccgggtgtc atgatcatac aggaagattt catagtcttc
    21481 cccgtcccac ccgtaccaga ccacacgatt cccgctgacc tgcggcgcca tgtcgtagaa
    21541 ttcattgctg gtgacctggg tggcttcgcc gttgttccag accatgattt cccagcccag
    21601 cggccactgc cggtctgtct gccacgcaat ggtggtgtcc gaaagagaag gatgggcgtt
    21661 gtcgcgcccg tcattggtga gcactttcac ttcgctcccg tcccagaata caatgttgtc
    21721 tccgcctctt cccggtttaa cttctttttt cggtgcgggt tcctccgggg ctgctgcagc
    21781 cgctgtttcg gtatctgcgg ggacggcgtt tgtgccggcg ggaacacgcg gctgcggtgc
    21841 gacatactcg gctcgcttgc tcccggcagt ccagactgcc ctgcttccat gcacacgggg
    21901 attgaggttc cctgccacaa gcgcggaccc ttcggtcagg ggcttgacgg tcccgttctg
    21961 gtatatgacc acttcggact cagcgttgtc cgtgccttcg ccggaaagcg actgccacgc
    22021 caccagacct gtgccgctga tgaccggatc acggttgatg cgggtctcgt ctgcgatctg
    22081 gcggatgtcg tactccgccc ctgctgtgaa ttggaataca atgcacagaa acagagccaa
    22141 ctgtgtaaag ctgtcgcgta tcctcaattt attccctggc cagaacatga atccttaaaa
    22201 tttgccttga atacataaca gaaaaaacaa gagaagggca acactatcct tacagaaaaa
    22261 tgcaggcctg cggccggccg tctctttccc tgaatcgttg gcttgtttcc cctgtgccga
    22321 tctggcagga tgactcgcgc gcaatgaagg attttcagac agacgaactg ggcatcatcg
    22381 ccggccgggg tgtttatccc cggatccttg cggcgtccgc caagcagcag ggtgtgcggc
    22441 gggtgttcgc cgccgctttc aggggtgaaa ccgaccggcg catctgccag gtggccgatg
    22501 aggtgtcctg ggttcatctg ggaagccttg gagccctgct ggatgccttc aaggcgagcg
    22561 gcgtcaggaa ggccgtcatg gccgggcaga tcaagcccac gcatctcttt cagcttcgca
    22621 tggactgccg catgattgat ctgctgaagc ggctccggga aagaaatgcc gacaccattt
    22681 tcggggcgct tgcggacgag atggcctcca tcggggttga acttctgccc gcttatcttt
    22741 tcatggaaag ctccatgccg gctcccggcc tgctgacccg caaggcgcct tcgcagaggg
    22801 aacagcagga tattgaactc ggcctgaagg tggccaaggc caccagctgc ctggaaatcg
    22861 gccaaacggt ggtggtcaag gagggaaccg ttcttgcggt ggaggctttt gagggaaccg
    22921 acaaggcgat tctgcgcgcc ggccggctgg gcggcagggg cgccgtggtg gtcaaggtgg
    22981 ccaagaaggg ccatgacatg cgttttgaca ttcccgtaat tggtttgcgc actctgaaga
    23041 cgctgcgcaa agcgaaaata tcctgtgtcg ccctggaggc aggccggacc attcttcttg
    23101 agcgcgagaa ggtgattgcc gaagccgacc ggatgggcct ctcgatgacg gctgtcgcca
    23161 tggactgaaa ggacgaagta tgccgaaaaa aacaatcagg gtcggtgtgg tgggcgtggg
    23221 cagtctcggc cagcaccatg cgcgcatcta ttccgaactg ccgggcgtgg aactggcggg
    23281 cgtttacgat gccagtccga agactgcttc cgcggtggcg gccaggcacc agacccgggc
    23341 ctgtgattcg ctggaggaac tggcctgcaa tatcgatgcc gccagtgtgg cggtgcccac
    23401 caccctgcat cgcgatgtgg ccgccgtcct gctgaaggcg ggggtgcatc tgctgattga
    23461 aaaacccatt gccgcgacca ccgccgaagc ggaggacatc gtggcgctgg cgcagaagca
    23521 ggacctgatc ctgcaggtgg ggcatatcga gcggttcaat ccggtcatga aattccttga
    23581 ggaagcctcg ccgaagccgc gcttcatcga ggcgcatcgc ctcgcttcct atccgccgcc
    23641 gcgcgagggg cttcatccgc gcggcacgga ggtcagtgtg attcttgacc tgatgattca
    23701 cgacattgaa atcattcttc atcttgtgaa ttcccgcgtg aaggacattc atgcggtcgg
    23761 cgtgccggtg ctcagtccca ccgaagacat tgccaacgtg cgtctcctgt tcgaaagcgg
    23821 atgcgtggcc aacatcacgg ccagccggat cagcagcgag cgcatgcgca aaatccgcgt
    23881 gttttccgag gatgcctata tctcgctcga ctaccagaat caggccggaa agatgttccg
    23941 gcgggagggc gggggaatcg ttcctgtgga ggtcccgatc gaaaaggggg accagctgac
    24001 cctggaactg gaatcgttca tcggatgcgt ggcgcgtcat ggggaccccg tggtgagcgg
    24061 ggatcacgcg gccgaggcgc tgcgcctggc catgcgaatc atcgcgatgg tgcagaaggg
    24121 cagttcatga gccggtccct gctggtcata gccggcgaag tctccggcga catgcatgcc
    24181 gccggggtgg ttcgggaact gcagaaacgc gatcccgaaa tgaagttctg gggtgtcggc
    24241 ggaagccgcc tccgcgagtg cgggatggaa attctttatg atgtgaagga catggccgtg
    24301 atggggctgg cggaggttct ccgacgctat ggttttttca agcgcgcact tgatcatgtg
    24361 ctggaagagg ccgcccgccg ccgcccggac gctgttcttc tggtggatta tccgggtttc
    24421 aatcttcgcg tggccgagca gctgaaaaag gccggcctgc ccgtgatcta ttatatctgc
    24481 ccgcaggtct gggcatggca tcgctcgcgc attcggaaga tcgcgcgcat tgtggatcac
    24541 ctgctcgtga tttttccctt tgaagtggat gttttcaaag acaccggcct gcgtgtggat
    24601 tttgtcggcc atcccctgac cgacgcgccg gaggaaacgg tttccgatct gccttggcag
    24661 ggctctcccc gtcttgcgct gcttccggga agccggaaac aggaaataca ccgaatcctt
    24721 ccggccatgc tcggagccgt ggaaaagctt cgggtggagc atccgaatct ggcggttctg
    24781 gtcgcctgcc cgtcggatca ggttgcgggc tgggtgcagg aattcgccgg tccccggaat
    24841 aatctgttca gggccgtcac gggaaagacc cgcgcggttc ttcgcacggc ccgtgccgcg
    24901 ctggtcaagt cgggcacctc aaccattgag gcggccctgg ccggctgtcc gatggtgctg
    24961 gtttacaaaa cctcctggct gacttatgcg ctcggacgcc tgcttgtacg tgttccgcac
    25021 atcggcatgg tgaacatcgt ggcgggttgc ccggttgttc cggaactgat tcagcagcag
    25081 gccactccgg ataccattgc acgggcggtc agcccgcttc tgtcccacac ctccgcgcat
    25141 gaccggatga agcaggatct atttgaggtc cagaagctta tgggtaaagg tggcgcctcg
    25201 cttcaggcgg ccgaactgat cctcgcggac atcgaaaact cccgcaagtt gtaaaaaatg
    25261 agaaatccgc ttgcgttgtg tttccagcag acttatcgta gaccccgaaa tctgataaga
    25321 gggctttatg gcgcgtattc ttttggttga tgatgaaccc agcattttga gtgtgttgag
    25381 cactctcctc cgcgcggagg ggtatgaagt ggttcccgct ctgggcggag aaaaagcact
    25441 cgaggaattg aagggaaaac ccttcgacct catgatttca gatatccgaa tgagccccat
    25501 tgacggaatg cagcttctca aggccgcgcg caaggactgg cctaaaatgg ccgtgctgat
    25561 gatcaccgcc tacggctcgg ttgaaaccgc cgtcggagcc atgaaagtcg gcgcctttga
    25621 ctatatcacc aagcccttca aggtggatga actgctcatc accgtgcagc gcgcccttga
    25681 atacaacaac gccatggccg aaaacgtcac cctcaagtcc cagctcgagg ctcgttacca
    25741 gtttgaaaac atcgtggccg aaagtcccgc catgcgcaag gtctgcgaga tgatcgagcg
    25801 cgtcgccccg accgatgcca ccgtgctgat tcagggggaa agcggaaccg gcaaggaact
    25861 cgtcgccaag gcaatccact cctacagcaa acgcaaaaac ggccgtttcg ttgccatcaa
    25921 ctgcgcggcc atgccggagc tgctgctgga atccgaaatg ttcggacacg tcaagggtgc
    25981 tttcaccggc gcgtcctccg acaaggaggg actcttcgaa gtcgccgccg gaggcacact
    26041 cttccttgat gaaatcggat ccatgccgct cagcattcag ggcaagctgc tgcgcgtgct
    26101 tcaggagcgc gaaatccggc gtgtgggtgg aaccgcgacc gtgcccgtgg atgtccgggt
    26161 gctcgccgcc tccaactgca tgctcgaaag cctcatcgaa gagggacggt tccgtgagga
    26221 cctctactac cgcctcagcg tcattcccat cgaggtggcc ccgctcacac agcgcaaaga
    26281 ggacatcctg ccgctggtct atcatttcct ccgcaccgaa atcggacccg gaaaggatct
    26341 gccggaaatt gacagcgatg cgcagctcat tctggaaaac tacacctggc ccggaaacgt
    26401 gcgcgagctg gaaaacgcca tccgccacgc cgtcaccttt gccaagggcg gcaaaattac
    26461 cgtggccgat ctgccggcca aaatatccgc cacggtcccg gcgacgcccg ccgccgccag
    26521 cagcccgtcc gcccagtaca agggcaaatc tctcaaggcc ttcctccgca ccaaggaaaa
    26581 agaatatctc tcgcaggtcc ttgaaacggc cggaggcgac aaggaggccg cggccaaggc
    26641 cctcaagatc agccttgcca cgctctaccg caagctgccg gaagactgat tttttccaat
    26701 cattggaact tcggtgcaaa aaacttccaa tgattggaac ttcttggaga aaaacttcca
    26761 atggttgaaa cttttgaaaa agtccgcctc ggcatcagca cctgcctgct ggggcagccc
    26821 gtccgctatg acggacagca caaactcgac accttcctgc gcgacacgct gggccgcttt
    26881 gtggaatatg ttcccgtctg ccccgaagtc gaatgcggac tgcccatccc gcgcgaggcg
    26941 atgcgccttg agggcaaccc ggataatccc cgtctgatga cccataaaac gcggcgcgac
    27001 atcactgaaa tgcagcaggc gtgggggcgg aaaaggctga aggaacttga atcggaagat
    27061 ctggacggat acatcttcaa aagcaaatcg cccagcagcg gactgttccg ggtgaaggtc
    27121 tatcaggaat ccggcatgcc cgtgaagcag ggcgttggaa tctgggccag aatgttcaca
    27181 gaaaggttcc ctttgcttcc ggttgaagag gagggccggc tgcatgatcc cgtcctgcgc
    27241 gagaatttca tcgagcgcat tttcgtgatg cgccgctggc ggaaaatgct cgcggaaaac
    27301 agtacgcgcg gcggactggt ggaattccat acgcgccaca agtatctgat cctcgcgcac
    27361 agctcgaagg cctacagtga tctcgggaaa atcgtggcgg gcatgaaggg ccggaatctg
    27421 aaagaagttc agtcggaata tctcgcaatc ctgatgccgg cgctgacggt gacggccacg
    27481 gtgaaaaaga gcgtgaatgt cctccagcac atgcagggct atttcaaaaa gcagctcacc
    27541 gctgacgaga aaaaggaaat gtcagaaacg ctgaacgagt atgcccgcgg attcatcccg
    27601 ctgatcgttc cggtcaccct catccggcac tatgtccgaa aatacggcga accgtacctc
    27661 gcggaccagt ggtatctgaa tccgcacccc ctcgaactcc agctccgcaa ccacgcctga
    27721 tcagtgtttc cgctggcagg acgggcaaat gtgcgtggag cgctggccaa ctttgatgcg
    27781 ttcaatgttt gtcccgcacc gcgggcaggg gagacctgtc tgacggaaca cgttcagtgc
    27841 ctcgcggttg cggccggatt caccgtccgg aagctgaaag tttgccttcc cgtgcccgag
    27901 cgatgttccc agattgcgga tgcccttttt cagcaccttc cggacagcct tcaaaagcgc
    27961 gtcggattcg gacgttgtga ggctgtctga cttccgctgt ggatgaatct ttgcctccca
    28021 taaagcctca tccacataaa tattccccag tccggcaatg aaggtctgat ccagcagaag
    28081 cggtttcagg atgcgcgact ttgcatgaag ccttttatgg aaatccctga ctggctccag
    28141 cggttcgggg cccagtttgc tcagaaccgt ttccgggtct tgtgtcagcc gccagcgccc
    28201 gaacttgcgt gtgtcgtgaa agcgcaaagt ccgtccgtcg tccagcttga ggagcacctg
    28261 atcatgtttt gtgatatcag cgggccggcc aaggaaaagc ctgccgctca tgcggagatg
    28321 gaccagagcg gtccatccgc cggacagacc catgaccacg aactttcccc tgcgactgat
    28381 attctggatc gtcctgccct tcagaatgcg cgtgaattct tcaaccgatg gctcctgaac
    28441 agaccgcatc cagtgtacag caacgctgga aatcctccgc ccgcagattc cctgatcccg
    28501 cagacttagc accaccgttt caacttctgg cagttccggc ataggtcaat attgccgaca
    28561 gatccgtttt cgtcaaagat gaaagcgcag gggcgctgca tctctgattc cggggcttgc
    28621 caagaagatg aacacgttgt agtttctcct gattcaaaag cggagagatg gccgagtggt
    28681 ttaaggcgca cgcttggaga gcgtgtgagg gtaacacctc cctgggttcg aatcccagtc
    28741 tctccgccat ttcttttaca tccccggatc aaacagattg agctggctgt cggacgcgcg
    28801 gggtttgcgc gggccgtgct ttgcgatttt aggctggccg gtttcgccca gttcgccttc
    28861 ctcaagattt gccagaattt cctttgcccg ttcaatgacc tgatccggca gtcccgccag
    28921 ccgcgcgacc tgaatgccga agctcttggc cgcgccgccg ggaacgattt tgcgcaggaa
    28981 gacgatgtgg tcgccttttt cgcggacgag cacgttgtag ttcttcacgc ccgccatggt
    29041 cagcgcgagg tcggtcagtt catgatagtg cgtggcaaaa agcgttttgg ctttcactgc
    29101 cggatggttg tgcagatgct cggccaccgc ccacgcgatg ctgatgccgt cgaaggtgct
    29161 ggtgccgcgc ccgatttcat cgagcacgat gaggcttttc ggcgtggcgt tgttcaggat
    29221 gttggcggtt tcctgcattt cgaccatgaa ggtgctgcgg ccgcgcgtga ggtcgtcgct
    29281 ggcgccgacg cgggtgaaga cgcggtcggt caggccgatt tcggctgatc ccgcaggcac
    29341 gaacgatccg atgtgcgcca tgatgacgat gagcgccacc tgccggatgt aggtggattt
    29401 gcccgccatg ttcgggcccg tgatgatgat gagctggctg gtcccgttgt cgaggatcg
//
LOCUS       DAOQWR010000015        27905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_1984_length_27905_cov_4.842226, whole genome shotgun sequence.
ACCESSION   DAOQWR010000015 DAOQWR010000000
VERSION     DAOQWR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27905
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1984_length_27905_cov_4.842226"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..356)
                     /locus_tag="PKK36_02375"
     CDS             complement(6..356)
                     /locus_tag="PKK36_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR93433.1"
                     /translation="MGGTDYKGRPLDLVQEVAYASGSVVSKTLMNKPVGSLTLFAFDA
                     GQGLSEHTSPYDAAVQILDGEAVINIGGSPLTARSGQMVIMPADVPHSLWAEKRFKML
                     LIMIRQPSAASDEG"
     gene            complement(359..646)
                     /locus_tag="PKK36_02380"
     CDS             complement(359..646)
                     /locus_tag="PKK36_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93434.1"
                     /translation="MKFKLMVAIMAVTAAAFVSYAGGPCCAEKAEKAKESGDEAAACK
                     AMTSGEEAAACKAMKKSGDKAAGCCMMKKAGCAPKLPDAPPAPEEAAAPAE"
     gene            complement(703..1482)
                     /locus_tag="PKK36_02385"
     CDS             complement(703..1482)
                     /locus_tag="PKK36_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNR93435.1"
                     /translation="MAKRKIIRINEDLCNGCGECVTSCAEGALKIVNGKAKLVKEQFC
                     DGFGDCIGECPTGALTIEEREADAFDFAATREHVAATEGPAAVTRFDEAGKKHGLAPS
                     GGCPGMRMRVPEAPAAKQAVAANAQMPTAIPSELQQWPVQLHLVQPGAPFFKNRELVV
                     MSTCGPIASADIHWRFLRGRGIVVACPKLDRTEGYVEKMADIFSEPTIPKVIVVRMQV
                     PCCGGLTMMVRAAAERCGRRDLVIEEAVMSLTGDVLSQKAI"
     gene            complement(1484..3127)
                     /gene="hcp"
                     /locus_tag="PKK36_02390"
                     /gene_synonym="priS"
     CDS             complement(1484..3127)
                     /gene="hcp"
                     /locus_tag="PKK36_02390"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNR93436.1"
                     /translation="MFCFQCQETAKNQGCTVRGVCGKQEPTANLQDLLIHTLKGLAIA
                     GKKADELGRYDREVGRTIIRGLFATITNANFDDAVISRLILEAVAQRDTLRKECEAKD
                     ATWAQKLHDAATWAPKKGEDLQVKALLVGIPSEPNEDVRSLQQLLIIGLKGIAAYADH
                     ALVLGYEDDAIYRFIVDGLISTTQKLSVDEMVGMVLKAGEIAVTTMALLDKANTETYG
                     SPEITKVSTGVRRNPGILISGHDLKDMDELLQQTEGTGVDVYTHGEMLPCHYYPAFKK
                     YKHLAGNYGGSWWLQDKEFASFNGPILLTTNCIIPVKDSYKDRFYTTGMAGWPGTKHI
                     PDRKPRKQKDFSPLIAQAKKCAPPQELENGEIVGGFAHNQVMAVAGKVVEAVKSGAIK
                     RFVVMAGCDGRQKSRNYFTEVAEALPKDTIILTAGCAKYRYNKLDLGDIGGIPRVLDA
                     GQCNDSYSLAVIALKLKEAFGLESINDLPLSFDIAWYEQKAVAVLLALLHLGVKGIRL
                     GPTLPAFLSPAVAKVLVDNFDIKPTGEVQADVEAMMAGK"
     gene            complement(3130..3549)
                     /locus_tag="PKK36_02395"
     CDS             complement(3130..3549)
                     /locus_tag="PKK36_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR93437.1"
                     /translation="MEILKRETDYALRALHLLDQGECTDTMALARDCKAPLASIRKIM
                     KKLANDGIVKAVRGVQGGYLLAMPSRRISLGRVVEVIQGPVYMNVCNRKGYACPYSGK
                     CPMKGSVCILQKRLNDFFNKTNLAEIYKQPKKRKGKS"
     gene            complement(3622..3993)
                     /locus_tag="PKK36_02400"
     CDS             complement(3622..3993)
                     /locus_tag="PKK36_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93438.1"
                     /translation="MKSAKDHCFKNCTTCGTSWPDRDSFLADSRVEFLGYQPMFEGRH
                     LGYFLFNHIMQCTTTLAVSVEEFRDLYDGPVWQECRYGGPDCPGYCAHLGQDGRCPAQ
                     CVCTWVREVMSILRAWPKKIS"
     gene            complement(4261..4446)
                     /locus_tag="PKK36_02405"
     CDS             complement(4261..4446)
                     /locus_tag="PKK36_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNR93439.1"
                     /translation="MTVENAVRILAGTLVLVSAALAYYVSPWWLLLTCFVGVNLIQSA
                     FTGFCPAEMLFRKLGLK"
     gene            complement(4462..5721)
                     /locus_tag="PKK36_02410"
     CDS             complement(4462..5721)
                     /locus_tag="PKK36_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93440.1"
                     /translation="MMKRYIFIAVFVLAAGVAQAANVHGFADVRAGLRMQNDPNQDDE
                     SLGELRLQLDVMEYAGPLTLQLRSDFLYDWVLDEHDIDLETGEGAIDLREANVSASPV
                     SWADVKAGRQILTWGTGDLLFINDMFPKDWNSFFIGRDEEYLKAPSDAVKVSLFPSWA
                     NIDLVYTPRFDHDRFITGDRISFWNAGLGARSGQDAVVDANIPDEWFDDDEISIRVSR
                     NMKGYELALYGYEGFWKSPSGSDMESGRAIFPRLGVYGASLRGQLMKGIANLEAGYYD
                     SRDDRDGDDPFVHNSEARLLAGYQQELMRDLTLAVQYYVELMMDYGEYEDSLPEGMDA
                     ADENRHVLTLRLTQQLLSQNLMLSFFAYWSPSDEDGYLRPSVSYKASDEWKFTAGGNI
                     FMGRDDHTFFGQFEDNNNVYLSARYSF"
     gene            complement(5730..6572)
                     /locus_tag="PKK36_02415"
     CDS             complement(5730..6572)
                     /locus_tag="PKK36_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNR93441.1"
                     /translation="MKSNPSIKYQASSIIHRLSLMSVAIFSVLLASLSSAAAEDLTVD
                     EIITKANEAAYYAGRDGQADVKMTIVDAGGGERIRQFRILRLNAENGNQKFYVYFNEP
                     ADVRRMVYLVWKKVGEGQDDDRWMYLPALDLVRRIAPGDKRTSFVGSDFVYEDVSGRN
                     LNADEHELISSAGGQYQIKNTPKDPGSVEFSYYTVWIDQDTFLPRKAEYYDKNGKLYR
                     RVEGTKVETVQGYPTVVESVVTDLNTGGKTVNAFSGMKYDIGLNDAIFSERFLKRPPR
                     EATR"
     gene            complement(6569..6934)
                     /locus_tag="PKK36_02420"
     CDS             complement(6569..6934)
                     /locus_tag="PKK36_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR93442.1"
                     /translation="MREIYEMDVYRLAEELSDLVWNDFDLWPEKARRTIGYQVIRFAD
                     SIAANIAEGYGRYSSAEKIQFYRYARGSFEETKAWLRKAIRRSVIEKKRTDEFKTIIG
                     ELGPKLNAFIRSTNKGANK"
     gene            complement(6973..9957)
                     /locus_tag="PKK36_02425"
     CDS             complement(6973..9957)
                     /locus_tag="PKK36_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNR93443.1"
                     /translation="MKRLAMTNFSVRHPWLVLLVVLAAVVGFGLQFPKVKFDNDPENM
                     LSPEEYVRVFHNRIKQRYNLYDFVIVGIVNTQHEDGIFNVGTLGRVDALTKQLISLHR
                     AGDGRPAVVNPGDGRTIIPDLRPAGAWKRALGAVFRHDVNNLFDEEGASAIITPEIMS
                     PSVVDNIKQAAMGQLKIEYLMEQPPQTREEALAIRDDAMGNPLYNGMLVSEDEKAVCV
                     YIPIVEKTYSYNVANLVEALTKDWPAEDRVLITGQPVAQDTFGVEMLVQMASSAPLAG
                     VAIFLLLLLFFRRVSLIIPPMIVAVVSVICTMGLLIGLGYDVHIMSSMIAIFLMPIAV
                     ADSVHILSEFFDTYHRFQDKSRTVQHVIGHLFMPMLYTSLTTIAGFASLATTPIPPVR
                     VFGLHIAFGVALAWFLTMTLVPAYIMIFVSKKTLDKACRVHDQESCDVKPSRLDRFLE
                     WLGAFSYARWKFIVAATLLVVGLAVYGISRIEVNDNPVKWFVKSHRIRIADDVLNQHF
                     GGTYTAYLTLASPNVEAVSCHEKAAIIRSEARKRFGDSMPAALERFLAQLDIQEQLQR
                     NVSSADLRKCFVSLVQAAEQIDAEASASWITLGDEINYLDPEGLTFEVLSSAVTSVEG
                     VPDEDRAVLLSRLEPHKNLTGAELQNAALAVCDEFTALSFSDFVFEMEADLSAPLFKR
                     PEVLAYIQALQDHLIRNEVIGKVSSAVDALKKANYELNWLEPSTDEDRARNQSFYSIP
                     PTPSACAQVFTQLEGSKKKDSLFHLLTKDYQQINIWVQLKSGDNKYMEAVVRDVEAYM
                     TANPPPGGLEAGWAGLTYLNVVWQDKMVVGMLSSLGSSFVIVLIMMMLLFRSPLMGLL
                     SMIPLTVTISFIYGLIGLAGKDYDMPVAVLSALTLGLSVDFAIHFLERARELQKQRGS
                     WKLAIREMFKEPAMAISRNAIVISLGFTPLLAAPLVPYKTVGFFMAAIMAVSWLATLF
                     ILAALVTALRRVLFKSDAGERD"
     gene            10154..11605
                     /gene="gnd"
                     /locus_tag="PKK36_02430"
     CDS             10154..11605
                     /gene="gnd"
                     /locus_tag="PKK36_02430"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNR93444.1"
                     /translation="MQKKADIGVIGLAVMGENLILNMESNGFAVACFNRTVDRMNQFV
                     SGRAAGKNIIGCRTLDELVAALERPRKVMMMVRAGSAVDALIEALLPLLEPGDIIIDG
                     GNSHYPDTIRRVQYVESRGLLYIGTGISGGEEGALTGPSIMPGGSPAAWPAVKPIFQK
                     IAARTSGGEICCDWVGEGGAGHFVKMVHNGIEYGDMQLIAEAYHMMKLGLRLSNEEMH
                     LIFANWSSGPLDSYLIEITSEILAYREDGEFLLDRILDAAGQKGTGKWTAAAALDLGQ
                     PLTLIAEAVYARCLSALKDERETASRVYNTPPAMVTGHTTESVQQLESALYASKIVSY
                     AQGYQLMRAMAEEQKWNLNYGGIALMWRGGCIIRSAFLGKIKEAYDRDPALANLLLDP
                     FFRDAVMKALPGWRRVVAGGAMTGIPLPAMSSALAWFDGYRSENLPANLIQAQRDYFG
                     AHTYERTDRPRGEFFHTNWTGRGGTTAAGTYGA"
     gene            11708..11950
                     /locus_tag="PKK36_02435"
     CDS             11708..11950
                     /locus_tag="PKK36_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR93445.1"
                     /translation="METKIQKWGNSLAVRIPKAFVKEAQLAYGSPVDLSFDDGKIVIN
                     PNPEPPFSLDDLLKGVKKRNLHSETGTGKPVGREVW"
     gene            11952..12293
                     /gene="mazF"
                     /locus_tag="PKK36_02440"
     CDS             11952..12293
                     /gene="mazF"
                     /locus_tag="PKK36_02440"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HNR93446.1"
                     /translation="MTSPHCPRRGDVVWLAFTPQSGHEQAGHRPALVLSPESYNRKVG
                     LALFCPITSQIKGYPFEVIIPKDCRVSGAVLSDQIKSLDWKARRASLICSLPESIVRQ
                     VLNKVGTLLDA"
     gene            complement(12514..12744)
                     /locus_tag="PKK36_02445"
     CDS             complement(12514..12744)
                     /locus_tag="PKK36_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNR93447.1"
                     /translation="MDGALIPRSTNGSSYQSGIWDLAQDTTYDLPYYNLSDVQGIYGT
                     LNVTPIPEPFTLVLLGLGSLLTVSLRRKPPAL"
     gene            complement(12871..15447)
                     /gene="topA"
                     /locus_tag="PKK36_02450"
     CDS             complement(12871..15447)
                     /gene="topA"
                     /locus_tag="PKK36_02450"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNR93448.1"
                     /translation="MGKKLVIVESPAKARTINKILGKDFLVKASMGHVRDLPEKQLGV
                     DIEKNFTPQYKVIKDKQKVIKELQNAAKDVETVYLAPDPDREGEAIAWHLQAALEKQV
                     PAENFLRVTYNEITAPAIREAFAHPRQIDQNRVNSQQARRILDRIVGYKVSPLLWSRV
                     RGGASAGRVQSVALRLVCEREKEIRNFVKEKFWVLGAKLRKLVDPRDVFETRLVKIGN
                     EKADLRDEAQAERIFEDVQNSAFRVQSIKDKETQKRARPPYITSSLQQAGSSVHGFSP
                     SRTMMIAQKLYEGVDLGEGRIGLITYMRTDSFNVSKVAQDQARDFIRKNFGDDYVPEK
                     PNFYKSRGTAQEAHEAIRPTDPSRTPESVQSALSPEEAKLYALIWKRFMASQMTPARI
                     AQRSVEIEALPKREGADPLLFRANASQVLFPGYMKASGLEKTKTQNEGEEEEDDTQGS
                     LPPLTEGEGLECVEPVRAEKETQPPPRYTEASLIRALEENGVGRPSTYAAILSTLNAR
                     KYIAREKKAILPTELGDRVCDFLVAHLSELFDVQFTAAMEKKLDDIESGEVEWHQMLG
                     EFYQNFLGWVQQAKGPSADPGHVGQVLGMLEHVREWAPGRQAGKREYSDEKFVESVRQ
                     QFEAGEKAVSQRQADALLKLGVKYKDQISGWDEFVVAQGLGEAIGAIEAKNLPPREET
                     SGKLDALADVAFKDARTIGKKTYDDRVFYESLKKQNDEGRRFSDRQVAALDKMLLKYA
                     DQIGGREQVVAQFGLVEQEKKPGEDSGPILALMESVQAWREPVKRGRREWDDHAFYQS
                     LKAQYAQKHELSFKQHMALKKMVVRYRDQIPSFAEEAEKQGLVIPQKKAPKGKNLEER
                     AE"
     gene            15683..17437
                     /gene="iorA"
                     /locus_tag="PKK36_02455"
     CDS             15683..17437
                     /gene="iorA"
                     /locus_tag="PKK36_02455"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNR93449.1"
                     /translation="MKTLMLGNEAIARGAWEAGVRVATAYPGTPSTEVTEYIAQYKEI
                     DAEWSVNEKVALEVAIGASVAGARAVCTMKHVGLNVAMDPLLSISYPGVNAGLVVVVA
                     DDPGQHSSQNEQDTRYFGMAGKLPVLEPSDSRECRDFMIRAFEISEEFDTPVIVRTTT
                     RVAHAYGLLEPGGRMDVPLREYDSRFGKYMVAPAFSRRRRVVAEERLGKLREFAETTD
                     LNIYENGSSDTCIITGSIAYQYCREVMPNAGFCKLGLSYPLPERKLKDFCGQHKKVFV
                     VEELEPIFEQHLKALGVPVIGKAKVPVIGELSTEIVRKGLTDTAPEPVSTPFENLPVR
                     PPVLCPGCHHRGPFYVMKKLKLKVAGDIGCYTLTASPPLSIMDTAICMGAGIGMAFGM
                     QKARGKDFAGKSIAVIGDSTFWHSGMTGLVDIVYNRGITTVVILDNSITAMTGHQENP
                     SSGRTLNGESAPALSFADFAKSIGIKHVREVDAFNLKELETVMREELAREEPSVIITK
                     RTCILHPCAVRHFPLKVNPGLCKSCMKCLSIGCPAISFDGDKVKIDQVLCVGCELCAG
                     ICPFHAIEKQPRPGGGPA"
     gene            17434..18033
                     /locus_tag="PKK36_02460"
     CDS             17434..18033
                     /locus_tag="PKK36_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNR93450.1"
                     /translation="MKDTTNILIAAVGGQGALLASKILGAFATARGDDVKMSEIHGMA
                     QRGGSVVTHVRFGEKVFSPTIEQGSADVLLAFEMLEAARHAAMLKPGGTVIVNTQTIA
                     PLPVLTNEMPYPADIIGNLKKMNLRVWHTDALDLARRAGHVKTVNTVLIGMYARMTGD
                     PAESWLKVLAENLPPKLYKVNQAAFTAGYNLSPPWTEQE"
     gene            18037..19338
                     /locus_tag="PKK36_02465"
     CDS             18037..19338
                     /locus_tag="PKK36_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNR93451.1"
                     /translation="MIWNEKCECMPRDELTALQTRRLQATVKRVYEKVPFYRRKMDEL
                     GVKPQDIKSIRDIRHLPMTTKQDLRDNYPFGLFSSPMSEIVRLHASSGTTGKPTVVGY
                     AREDIELWADVMARTLCSAGGTEHDIIQISYGYGLFTGGLGVHYGAEKLGATAIPVSG
                     GNTQRQIQILQDFGPTLLACTPSYSLFLAEALAERGIHPGDLKLKAAVLGAEPWTENM
                     RAAIEKNLGIRAIDIYGLSEIIGPGVASECWEQKGLHINEDHFYPEIIDPVTLEVLEP
                     GQHGELVFTTITKQGMPLLRYRTRDLTSLITGACPCGRTTVRMHKCQGRSDDMLIIRG
                     VNVFPSQIESVLLEMSETHPHYLLIIDRVNNLDQLTLQVEVDAQFFSDEIKQLEALSR
                     RIKHSVESTLGLSVNVRLVEPQTIERSEGKARRVIDNRKLS"
     gene            19351..19776
                     /locus_tag="PKK36_02470"
     CDS             19351..19776
                     /locus_tag="PKK36_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNR93452.1"
                     /translation="MIIQQVSVFLENKSGRLVEVTQALADAGVNISALSIAETSDYGV
                     LRCVVSHPQKAVELLRARHFSVSLTDVVCMAAPNQPGALNRALKILAEAGISVEYMYA
                     FAIGDRALVVIRTDNIKDTISALQHHKVDLLKSSELYQV"
     gene            complement(19777..21144)
                     /locus_tag="PKK36_02475"
     CDS             complement(19777..21144)
                     /locus_tag="PKK36_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR93453.1"
                     /translation="MKIFAARRNREVLTEGGLLKAIAVLAVPMLAGAVLQNVQSLIDL
                     FWVGRLGSRAVAAVAMSGTILMVLFPMLMGLSTGTIALVSRSVGAGRPEDAAAAAGQS
                     LMLAVVLGGITAACGWLFSPHLFRLLGAEPELIHDGSIYLRICLIGSFTVFILFIGNA
                     ALQGAGDAVTPMCIMAVSNVVNIILDPLLIFGPGPFPCLGIRGAASATVISQAAAAGL
                     SLYILFTGKTHLRIHLKQWIPRAELSWRILRIGLPGSGQMLSRSLMSAVLMRIVASCG
                     TAAVAAYGVGMRFHMIALMPAFALGGAAATMVGQNLGAGKPARARRAAWAAVGLDMVF
                     MTGIAAVLMIFAPALIRFFNSEPDVVRTGTDYLRIVSPSYIFAALGIVFGRALNGAGD
                     SMSPMIITVATLWGLQVPLAVMLSRIWEPATSGIWWAIVAAIIFQGLLAAWWFERGTW
                     SRKTV"
     gene            complement(21247..21948)
                     /locus_tag="PKK36_02480"
     CDS             complement(21247..21948)
                     /locus_tag="PKK36_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR93454.1"
                     /translation="MTSSRQIILRLKALGSDEIARHSQRFFKTGKGEYGEGDLFLGIR
                     VPVIRQCVKEWRDLPLDVVLEILQSPFHEARLLALLIMVKQYAAGSEQARNALYRVYL
                     HHTACINNWDLVDCSAEHVVGAHLFTRSRAPLYRLAKSESLWERRIALLATFHFIRRN
                     DFEDLLKLAEMLLPDREDLIHKAAGWMLREVGKRDRSIETEFLERHARSMPRTMLRYA
                     IERFPESARQRFLHQ"
     gene            complement(21948..23147)
                     /locus_tag="PKK36_02485"
     CDS             complement(21948..23147)
                     /locus_tag="PKK36_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNR93455.1"
                     /translation="MYRVNSFLGLILMMLLAWLLSSDRRRMDFRLILSGVGLQILFGV
                     FIMKTSVGLKIFDVARAVISRLISFSDDGAQFVFGEQFREHYFAFSVLPTIIFVSSLM
                     AILFHLGIMQHVVSFMARVMVKVMDASGSESLAAAANVFIGQTEAPLVVKPYLLTMTR
                     SELMALMTGGMATVAGGVLAAYAGMGADPGHLLTASVMSAPASLVLAKIIVPETEESN
                     TRGYVRIQVPKQDSNVLDAACRGASEGLMLALNVAAMLIAFIALVGAFNWLCTLLPDI
                     GGAPLTLQRVFSWMMAPFALMMGVEWQDAGIAGTLLGEKTVLNEFVAYVDLIKWRDQV
                     SPRTFTLMTYALCGFANFSSVAIQIGGIGSLVPSRRRDFAKLGLKAMVAGSLAAFMTA
                     CIAGMLL"
     gene            23298..25658
                     /locus_tag="PKK36_02490"
     CDS             23298..25658
                     /locus_tag="PKK36_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNR93456.1"
                     /translation="MELLSFVLLVLMIVVLAKLSSIRGALKNIESEQSNLRRMVKKLL
                     PAEEPSKPWTPPPRQEIPAPVPSPAPAVPEPPRVAAAPQTPPAVPSMPAYPRITRQPS
                     RVGEILRKIWSWILVGEEHRREGVSAEFAIASTWLLRAGIVAIVTCVGFFLKWSIERQ
                     LLGPTGRTAIAIAAGIGMLAAGYRLFGKKYHLMGQGLFGGGLATLYFSMYAAGPLYKL
                     LPIPAAFLLMILITITAGLLSVRVNSLLVAILGIIGGFCTPIMLSTGKPDFMVLYVYM
                     LILGAGVFALSQVKSWRLLNYLSFFFTWMLVLGSLFSYRKPDFNLVMPLLSLLFALHA
                     AIVIWHNIGRNGKLTVLEIIYLLINSGLFSLTAYSLIRYTHGRPYPAILTLALAVFYI
                     GHVLAFLQFKRGDRALLITMMALAGFYTTLTMPLVLERESLTMSWSLLALMFLWLSGK
                     VQSRFLNRLSYLLYGLVFFKLAAVDLHENFPHPSMGPGGTFKLYLKSMTDRLWTFGVS
                     IASVFGAFWLERRRPQPGPEAGPEETPSRGLLPTLFYWIGLSVLLIYLLFETNAFFRF
                     YAPWRLPMMTVVFCVMGLYTLWRFFATDDRSHLIASNILLGLAAAKLLLWDIPHWDIS
                     RGGLYGCEYSSVMVLSRLLDFGLLLVILFGVWALFVRRDSRKSLAPFYGYGGLALLFL
                     YTTLELNAFLHFKEPSFRAGGVSVLWAIYAVCFVAGGIWKKLRGLRLAGLLLFAVVTA
                     KVFLHDLAGMPAIWRVAAFFVVGITLLIGAFAYLNAAPGFIQKKDE"
     gene            25662..27026
                     /locus_tag="PKK36_02495"
     CDS             25662..27026
                     /locus_tag="PKK36_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93457.1"
                     /translation="MKRLLAVSIFTFSLAHCADARISLRDYPWMKPLIRPDGPQAEVA
                     SFTLDNPMYAATDDQYANIRITDENGAEVPYGLRKKVYAVTQIVERATASEIVSFDRQ
                     ANNSVNVVLRKKDAKARPEAVIIHTRHRNFEKPVRIFGSRDGASWELLKESRIYDYSR
                     HIDVRSLRTDFPPSDHTLFRIEIGNFIEQVPDATTGISTERRGDAVFSEIEHRALQAE
                     TIRIESVELRTKERVIRPDSAHVKVYESRDLRMSSSEEKQTTSWTFGTDRQPLTSLSF
                     ESASRNFSRPFTVEGRRGSREEAWRTLGSATLHRFELGGIRDENLTVMLGGDCRYDEY
                     RVTVFNHDNASLDISGVVATGYVYEVLFLPDPGADYTVLYGGSGIPRPRYDVETVLSR
                     VPTIESTVMSLGEQKANPKYKPGPRWDGKTSKILFIAAVFGMVILLGWFIAKGAGKIE
                     AMEE"
     gene            27351..>27905
                     /locus_tag="PKK36_02500"
     CDS             27351..>27905
                     /locus_tag="PKK36_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR93458.1"
                     /translation="MRILVTGGTGFTGKALVRRLLNDGHSVIAMDYKEGIKTQELRDW
                     GAEVVIGSVTDREVVKQCMKGVEVVQHVAAAFREMNVPESYYDEVNVGGTRICLEEAM
                     AAGVKKFIYCSTCGVHGNVKNPPTDEAHNIAPADYYQQTKYNAEPVVKSFHEKGLPCV
                     ILRPSAIYGPGDPERFFMIYRRAAK"
BASE COUNT         5709 a         8590 c         7914 g         5692 t
ORIGIN      
        1 caagcttatc cttcgtctga agccgccgac ggctggcgga tcatgatcag cagcatcttg
       61 aaccgctttt cggcccagag agaatgaggc acatccgccg gcatgatcac catttggccg
      121 gaccgggcag ttagagggct cccccctata ttgatcaccg cttctccgtc cagtatctga
      181 acagccgcat cataggggga tgtgtgctcg ctcagcccct gtcccgcatc gaaggcaaac
      241 agagtgagag accccaccgg cttgttcatc agagtcttgc tgacaaccga accggaggca
      301 taggcgacct cctgcaccag atcgagcggc cggcctttgt aatccgtgcc gcccatgatc
      361 actcagcggg agcggccgct tcttcaggag caggcggggc atcgggaagt ttcggggcac
      421 aaccggcctt cttcatcatg cagcatccgg ctgctttatc ccctgacttc ttcatcgctt
      481 tgcaggccgc cgcttcctcc ccggacgtca tcgccttgca ggcggcagct tcatcgccgg
      541 actcttttgc cttttccgcc ttttcggcgc agcagggtcc gcccgcatag gaaacaaacg
      601 ccgcagccgt gaccgccatt atcgcaacca tcaatttaaa cttcatcaca caatcctttc
      661 ttcccttgtg ggagggtaag catacgcgca atccgggaga attcaaatcg ccttctgcga
      721 aagaacatct ccggtcagac tcatgaccgc ctcttcaatg accagatctc tgcggccgca
      781 tctttccgcg gcagcccgaa ccatcatggt gagccccccg cagcagggca cctgcatccg
      841 gaccacaatc actttgggga tggtgggctc tgaaaatatg tcggccattt tttcgacata
      901 tccctccgtg cggtccagtt tggggcaggc cacaacgatc cccctgcccc ggaggaaacg
      961 ccagtgtatg tccgccgaag cgatcggccc gcaggtgctc atcaccacca gttcccggtt
     1021 tttgaagaac ggcgcgccgg gctgcaccag atgaagctga acaggccact gctgcagttc
     1081 cgacgggatg gccgtcggca tttgcgcatt tgcggcaacg gcctgcttcg cggccggggc
     1141 ttccggcaca cgcatgcgca tgccgggaca accgccggac ggagccagcc catgcttctt
     1201 cccggcctca tcaaagcggg tcacggcagc agggccctcg gttgccgcca catgttcgcg
     1261 ggtggcggcg aagtcgaagg cgtccgcctc gcgctcttca atggtcagag ctccggtcgg
     1321 acactctcca atgcagtctc cgaatccgtc gcagaactgc tccttgacca gcttcgcctt
     1381 gccgttgacg atcttcaatg cgccctctgc gcaggatgtg acacattcgc cgcatccgtt
     1441 gcagaggtcc tcattgatcc tgataatctt tcgcttcgcc atatcacttc ccggccatca
     1501 tggcctcgac atccgcctgc acctcgccgg tgggcttgat gtcaaaattg tccaccagca
     1561 ccttggccac cgcgggcgag agaaacgccg gcagcgtcgg ccccagccgg attcccttca
     1621 cccccagatg cagcagcgcc agcagcacgg ccacggcctt ctgctcgtac caggcgatgt
     1681 cgaaagacaa cggaagatcg ttgatggatt ccagtccgaa ggcctccttc agtttcagcg
     1741 cgatgaccgc cagcgaatag ctgtcgttgc actgcccggc atccagaaca cgcgggatcc
     1801 cgccgatatc gccaaggtcc agcttgttgt agcggtattt cgcgcagccc gcggtcaaaa
     1861 tgatcgtgtc cttcggcagg gcctcggcca cttcggtgaa atagttccgg gatttctggc
     1921 gcccgtcgca tccggccatc accacaaaac gtttgatcgc tccggacttt acggcctcca
     1981 ccaccttgcc ggcgacagcc atcacctgat tatgagcgaa gccgccgacg atttcgccgt
     2041 tttccagctc ctgcggaggc gcgcatttct ttgcctgcgc gatgagcggg gagaagtcct
     2101 tctgttttcg cggtttccgg tcaggaatat gcttcgtgcc cggccagccg gccatgcctg
     2161 tcgtgtagaa acgatccttg tagctgtcct tcaccggaat gatgcagttg gtcgtcagca
     2221 ggatcggccc gttgaatgag gcgaattcct tgtcctgaag ccaccagctt ccgccgtagt
     2281 ttccggccag atgcttgtat ttcttgaagg ccggatagta atggcacgga agcatttcgc
     2341 cgtgtgtata gacatccacg cccgtgccct cggtctgctg caggagctcg tccatatcct
     2401 tcaggtcgtg gccggaaatc aggatgcccg gattcctgcg caccccggtt gacactttcg
     2461 tgatctccgg actgccgtag gtctccgtgt tggccttatc gagaagcgcc atggtcgtga
     2521 cggcaatttc gcccgccttc agcaccatgc ccaccatctc gtccacgctg agtttctgcg
     2581 tggttgaaat cagtccgtcc acgatgaacc ggtaaatggc atcatcctcg taccccagca
     2641 ccagcgcatg atcagcgtag gctgcaatgc ccttgaggcc gatgatcaga agctgctgca
     2701 gcgaccgcac atcctcgttc ggctcggagg gaatgccgac cagaagagcc ttcacctgaa
     2761 ggtcttcccc ctttttcgga gcccaggtcg ctgcgtcatg cagtttctgt gcccacgtcg
     2821 cgtctttcgc ctcgcattcc ttgcgaagcg tgtcgcgctg agcaacagcc tccaaaatga
     2881 ggcggctgat gaccgcatca tcaaaattgg cgttggtgat cgtggcgaac agcccgcgga
     2941 taattgtccg gccgacctcc cggtcatagc ggccgagttc atcggccttt ttgccggcaa
     3001 tggccagtcc tttcagagtg tggatcagaa gatcctgcag attcgccgtg ggctcctgtt
     3061 tcccgcatac cccgcggacg gtgcaaccct ggtttttcgc ggtttcctga cactggaagc
     3121 agaacatgct catgactttc cctttctctt ttttggttgt ttgtagattt cagctaaatt
     3181 ggttttgttg aaaaaatcgt tcaggcgctt ctgcagaatg cagacggagc ccttcatcgg
     3241 gcatttcccg ctgtacgggc acgcatagcc cttcctgttg cagacgttca tgtacaccgg
     3301 accctgaatg acttcaacca cccggcccag actgatcctg cgcgagggca tcgccagcag
     3361 atatcccccc tgcacgccgc gaacagcctt gacgattccg tcattcgcca gcttcttcat
     3421 tattttgcgg atggatgcca gcggcgcctt gcagtcgcgc gcgagggcca tcgtgtccgt
     3481 gcactccccc tggtccagca gatgcagcgc ccgcagtgca taatcagtct ctcttttcag
     3541 tatttccatg gatgctcctt taatatagta ctaaaatagt actatttaaa cgcgatgtca
     3601 accggggcct tgaaatttgt ttcaggatat ctttttcggc cacgccctga ggatgctcat
     3661 gacctcccgg acccaggtgc agacgcactg ggccggacac cgcccgtcct gtcccagatg
     3721 cgcgcagtag cccgggcagt ccggtccccc gtaccggcat tcctgccaga ccggcccgtc
     3781 gtacaaatcc cggaattcct ccacggaaac ggcgagcgtg gtggtgcact gcatgatgtg
     3841 attgaaaagg aaatagccca gatgccgccc ctcaaacatc ggctgatatc ccagaaactc
     3901 cacacgggaa tcggccagga acgaatccct gtccggccag ctggtgccgc acgtcgtgca
     3961 gttcttgaaa caatggtcct tcgcgctctt cactatttcc tctgttcttt aaaatctaat
     4021 ggacgcgccg tcactttgca agaactgcgc agtctgctcc ttcggctcat gacccgaaaa
     4081 gttccagtca ttggaagttt tcagaccaaa agttccaatc attggaactt ttcagacaga
     4141 aagttccagt cattggaagt tttgcgcaag ggcggatttt cagcgggacg cggactgcca
     4201 cagggcccga acacggccgc atattcgcga aggagcggcg gatgtattcg cgcggatatc
     4261 ttacttcagg cccagtttgc ggaataacat ttccgccggg cagaagcccg tgaaagcgga
     4321 ctgaatcagg ttgactccca cgaagcaggt cagaagaagc caccacgggc tgacataata
     4381 tgccagcgcc gcgctgacca gcaccagtgt tcccgccaga atccgtactg cattttcaac
     4441 ggtcatattt tctctcctga tttagaaact gtaccgggcg gacagataga cattgttgtt
     4501 gtcctcaaac tgcccgaaga aggtgtgatc gtcccggccc atgaagatat ttcccccagc
     4561 cgtgaatttc cattcatcgg atgccttgta ggaaacggac ggacggagat agccgtcctc
     4621 gtctgacggc gaccagtagg cgaagaacga gagcatcagg ttctggctga gaagctgctg
     4681 cgtgagccgc agggtcagca cgtgccggtt ctcgtccgcc gcatccattc cctccggaag
     4741 cgaatcctcg tactcgccat aatccatcat cagctcgaca tagtactgaa ccgccagcgt
     4801 caggtcccgc atcagctcct gctggtaacc ggccagcagg cgggcttcac tgttgtgcac
     4861 gaaaggatcg tcgccgtcac ggtcgtcgcg agaatcgtaa tagcccgctt ccaggttcgc
     4921 gatgcccttc atgagctgtc cccgcagact tgcgccgtac actccaagcc tggggaatat
     4981 cgcacgaccg gattccatgt cggaccccga cgggcttttc cagaaacctt catatccgta
     5041 aagggccagc tcatacccct tcatgttgcg cgacacgcga atgctgattt catcgtcatc
     5101 aaaccattca tccgggatat tcgcatccac gacggcatcc tgtcccgacc gcgcgccgag
     5161 acccgcgttc cagaacgaaa tgcggtcgcc ggtgataaac cggtcatgat cgaagcgcgg
     5221 ggtgtacacc aggtctatgt tcgcccacga cgggaagagg ctcaccttga ccgcatcgga
     5281 cggcgccttc agatattcct cgtcacgccc gatgaagaag gaattccagt ccttgggaaa
     5341 catgtcgttg atgaacagca gatcgccggt gccccaggtc agaatctgcc ggccggcttt
     5401 cacatcagcc cagctcaccg gcgaggcaga gacgttggcc tcgcgcaggt caatggcccc
     5461 ctcgcccgtt tcgaggtcaa tatcatgctc gtccagcacc cagtcataca ggaaatccga
     5521 gcgcagctgc agcgtcaggg gtccggcgta ttccatgaca tcgagctgca ggcggagttc
     5581 gccaaggctt tcatcatcct ggttcggatc gttctgcatc cgaagcccgg ccctcacatc
     5641 ggcgaatccg tgcacattag ccgcctgcgc cacacctgcc gccagcacga agacagcgat
     5701 aaaaatatac cttttcatca ttttaatcct caacgggtgg cctcgcgcgg cggccgtttc
     5761 aaaaagcgtt ccgagaagat ggcgtcgttc aggccaatgt cgtatttcat cccgctgaac
     5821 gcattcactg ttttcccgcc ggtgttcaag tccgtcacga ccgattccac aacggtcgga
     5881 tatccctgaa cggtctcgac cttcgtgccc tcaacgcgcc ggtagagttt tccgttcttg
     5941 tcatagtact ccgccttgcg cgggaggaat gtgtcctggt ctatccagac agtataatag
     6001 gagaattcaa ccgagccggg atccttcggg gtgtttttga tctggtactg cccgccggcc
     6061 gatgaaatca gttcgtgctc atccgcattc agattgcggc cggagacatc ctcgtaaaca
     6121 aaatcggagc ccacaaagct ggtgcgcttg tcgccgggag cgatgcggcg gaccaggtcc
     6181 agcgcgggca gatacatcca gcggtcatca tcctgcccct cgcccacttt cttccagacc
     6241 agatacacca tgcggcgcac atcggccggc tcattgaagt agacgtagaa cttctggttc
     6301 ccgttctcgg cgttcagccg caggatgcgg aactggcgga tccgctcgcc gcccccggca
     6361 tccacaatgg tcattttaac atcagcctgg ccatccctgc ccgcataata cgcggcctcg
     6421 ttggcctttg tgataatctc atccaccgtc aggtcttcgg ccgcggcgga cgagagagaa
     6481 gccagcagga cagagaagat cgcaaccgac ataagggaga gacggtggat gatgcttgat
     6541 gcctgatact tgatgcttgg attggatttc atttattcgc tcctttattt gtgcttcgta
     6601 taaacgcatt cagtttgggc cccagctcgc caattattgt tttgaattca tcggttctct
     6661 tcttttctat tacactgcgg cgaatggcct tgcgcagcca cgccttggtc tcttcaaaag
     6721 aaccgcgggc ataacgataa aattgtatct tttcagcgga agaataccgt ccatagcctt
     6781 ccgcaatatt tgcagctatg ctgtcggcaa aacggatcac ctgataacca atcgtccgcc
     6841 gggccttttc gggccaaaga tcaaaatcat tccagacaag atcggacagc tcttcggcca
     6901 accgataaac atccatctca taaatctcgc gcatatctct tcttcccagt atcaagtttc
     6961 aagcatccag catcaatccc gctccccggc atctgatttg aacaagaccc tccgcagtgc
     7021 cgtcaccagc gcagccagaa tgaacagcgt ggccagccag gacacagcca tgatggcggc
     7081 catgaaaaag ccgacggttt tgtagggcac aagaggcgcc gccagcagcg gcgtaaatcc
     7141 aagtgaaatc acgatggcat tgcggctgat ggccatcgcc ggctccttga acatttcgcg
     7201 gatcgccagc ttccagctcc cgcgctgctt ctgcagctcg cgagctcgtt ccaggaagtg
     7261 aatcgcaaag tccacactca aacccagcgt cagcgccgac agcacggcca ccggcatgtc
     7321 gtagtccttg cccgccagcc cgatgaggcc gtagatgaat gaaatcgtca ctgtcagcgg
     7381 aatcatcgaa agcaggccca tcagcggcga gcggaacagg agcatcatca tgatcagtac
     7441 aatcacaaag ctgctgccga gtgatgaaag catgccgacc accatcttgt cctgccagac
     7501 cacgttcaga taggtgagcc cggcccagcc ggcctccagc ccccccggcg gcggattggc
     7561 tgtcatgtat gcctccacat cccggacaac cgcctccata tacttgttgt caccgctctt
     7621 gagctgtacc cagatgttga tctgctgata gtcctttgtc agcagatgaa acaaactgtc
     7681 ttttttcttc gaaccttcca gctgagtgaa cacctgcgcg caggcggaag gcgtgggcgg
     7741 aatcgaatag aaggactggt tgcgggcgcg gtcttcatcg gtggacggct ccagccagtt
     7801 aagctcgtaa ttcgccttct tcagggcgtc caccgccgaa ctgaccttcc cgatgacctc
     7861 attccggatc agatggtcct gcagcgcctg aatataggcc agcacttccg ggcgcttgaa
     7921 cagaggggcc gataaatccg cctccatctc aaacacgaaa tcgctgaagg aaagggccgt
     7981 gaattcatca caaacagcca gcgccgcatt ctgcagttcc gctccggtca gatttttatg
     8041 cggttccaga cgagacagca ggaccgcccg gtcctcatca ggaacgccct caacggacgt
     8101 cacagccgag gagagaacct cgaatgtcag cccttcggga tcgagatagt tgatctcatc
     8161 ccccagcgtg atccacgagg cggaagcttc ggcatcaatt tgttccgccg cctgcaccag
     8221 actcacaaag catttccgca ggtcggccga actgacattg cgctgaagct gctcctgaat
     8281 gtccagctgg gccagaaagc gctccagagc ggccggcatg ctgtcgccga accgcttccg
     8341 cgcctcgctg cggatgatgg ccgccttctc atggcagctt accgcctcaa cattcggaga
     8401 ggccagcgtc agataggcgg tgtaggttcc gccgaagtgc tggttcagca catcatcggc
     8461 gatccggatg cggtgcgatt tcacgaacca cttcacggga ttgtcgttca cttcgatgcg
     8521 cgaaatgccg tagacggcca gaccgacaac cagaagcgtg gccgccacaa tgaatttcca
     8581 gcgcgcatac gagaatgcgc ccagccattc caggaaacga tcaagcctcg acggcttaac
     8641 atcgcagctt tcctgatcat gcaccctgca ggccttgtcg agcgtctttt tcgagacaaa
     8701 gatcatgata taggccggca ccagcgtcat ggtgagaaac catgccaggg ccacgccaaa
     8761 cgcaatgtgc agtccgaaaa ccctgaccgg cggaatcggc gtggtggcca gcgacgcaaa
     8821 cccggcgatc gtcgtcaggc tggtgtagag cataggcatg aaaaggtgtc caataacatg
     8881 ctgaaccgtt ctgcttttgt cctggaaacg gtgataggtg tcgaagaatt cgctgaggat
     8941 atgcaccgaa tccgccacag cgatcggcat caggaaaatg gcgatcatcg agctcatgat
     9001 gtgcacgtcg tagcccagcc cgatcagcag gcccatggtg cagatcacgc tgacgaccgc
     9061 gacaatcatc ggtggaataa tcagcgacac gcggcggaag aacagcagca gaagcaggaa
     9121 aatggccacc ccggcaagcg gcgccgaaga cgccatctgc accagcatct ccaccccgaa
     9181 agtgtcctgc gcaaccggct ggccggtgat cagcacccgg tcctccgccg gccagtcctt
     9241 ggtgagggcc tccaccagat tggccacatt gtagctgtag gttttttcga cgatggggat
     9301 atagacgcac acggcctttt cgtcttcaga taccagcatc ccgttataaa gcgggttccc
     9361 catggcgtcg tcgcggatcg ccagcgcctc ctcgcgggtc tgcggcggct gttccatcag
     9421 atattcgatc ttaagctgcc ccatcgcagc ctgcttgatg ttgtccacca cgctcgggct
     9481 catgatttcg ggcgtgatga tggcgctggc cccctcctca tcgaagagat tattgacatc
     9541 atgcctgaaa acggcgccca gtgcgcgctt ccatgcgccg gccgggcgaa gatccggaat
     9601 gatcgtccgg ccgtctccgg gattcaccac tgccggccgg ccgtctcccg cccggtgcaa
     9661 actgatcagc tgtttcgtca gcgcgtccac ccgcccgagg gtcccgacgt tgaaaatgcc
     9721 gtcctcgtgc tgcgtattga caattccgac aatgacaaag tcatacaggt tgtagcgctg
     9781 tttaatccgg ttgtggaaca cccggacata ttcctccggg gaaagcatgt tctcgggatc
     9841 gttgtcgaac ttgacctttg gaaactgcag gccgaacccc accacggcgg cgagcacgac
     9901 cagcagaacg agccacggat gccgcacgct gaaattcgtc attgccagtc ttttcatatg
     9961 ccaaatctcc cgattatcct gagcgtttat accataaaac cggtccgccc gaagaaaatt
    10021 atggctcaat atatccatat atcatgatat gtcaatatat aaggctcatt ttctgccgtt
    10081 ttgccttttg ctttgaatcc cgcccggcct tcgcctatgc tgggccccag attcatcaag
    10141 tacggagtat cgcatgcaga aaaaagcgga tatcggcgtg atcggcctcg ccgtcatggg
    10201 cgaaaatctc attctgaaca tggagtcgaa cggctttgcc gtcgcctgct tcaaccgcac
    10261 ggttgaccgc atgaaccagt ttgtctccgg ccgggccgcg gggaagaaca tcatcggatg
    10321 ccggacgctc gacgagctgg tcgccgcact tgaacggccg cgaaaagtca tgatgatggt
    10381 gcgggccggt tccgctgtgg atgccctgat tgaggcgctc cttcccctgc ttgaacccgg
    10441 agatatcatt attgatggag gcaactccca ctaccccgac accatccgtc gcgttcaata
    10501 tgttgaaagc agggggctgc tgtacatcgg caccggcata tcgggcggcg aggaaggtgc
    10561 gctgaccggc ccatccatca tgcccggcgg atcgccggcc gcatggcccg ccgtgaagcc
    10621 gattttccag aaaatcgcgg ccaggacttc tggcggggaa atctgctgcg actgggtcgg
    10681 cgaaggcggt gccgggcatt tcgtcaaaat ggttcacaac ggcattgaat atggagacat
    10741 gcagctcatt gccgaagcct atcacatgat gaagctgggc ctgcggcttt ccaatgagga
    10801 aatgcatctg atatttgcca actggagcag cgggccgctt gattcctatc tgattgagat
    10861 cacgagcgag attctggcct atcgggagga tggcgaattt ctgctcgaca ggattctgga
    10921 tgccgccggt cagaaaggca ccggcaaatg gacggccgcc gccgcgctgg atctgggtca
    10981 gccgctcacg cttatcgccg aggctgtcta tgcccggtgc ctttcagccc tgaaagatga
    11041 aagggaaacc gccagcaggg tgtataacac gccgcccgcc atggtcacgg ggcacaccac
    11101 cgaatcggtg cagcagctcg aatcagcgct ctatgcgtca aagattgttt cctatgccca
    11161 gggctatcag cttatgcggg ccatggctga ggagcaaaaa tggaatctca actacggcgg
    11221 gattgccctc atgtggcgcg ggggctgcat tatccggtcg gctttcctgg ggaaaatcaa
    11281 ggaggcgtac gaccgtgatc cggcgctggc caacctgctg ctggatccct tcttcaggga
    11341 tgcagtcatg aaggcgcttc ccggctggcg gcgcgtggtg gccggaggag ccatgacggg
    11401 cattccgctg ccggcgatga gcagcgcgct ggcatggttt gacggatatc gaagtgaaaa
    11461 ccttcccgcc aatctgatcc aggcccagcg cgactatttc ggcgcccaca cctatgagcg
    11521 cacggaccgg ccgcggggcg agttcttcca taccaactgg accggccgcg gcggaaccac
    11581 cgcagcggga acctatgggg cataaagcaa gccaacccga agccgcaagc cggctccatc
    11641 tcgtgaaatt cccttgcacg ccgctcaacc atgacgtata tatattgtat atacatttga
    11701 ggtgtttatg gaaactaaga ttcagaaatg gggcaacagt ctggcagtgc ggattccgaa
    11761 ggcgtttgtc aaggaggcgc agctggccta tggttcgccg gttgatctgt cgtttgatga
    11821 cggcaaaatc gtcatcaatc cgaatcccga accgcccttc agtcttgatg accttctgaa
    11881 gggtgtcaaa aagcgcaatc ttcattccga aaccggaacg ggaaagcctg tcggacggga
    11941 ggtctggtaa aatgacttcc ccgcattgcc caagacgcgg cgacgtggtc tggctggcct
    12001 tcacgccgca gtcgggacac gagcaggccg gccatcgccc ggcgctcgtt ctttcgccgg
    12061 agtcatacaa ccgcaaggtc ggtcttgcgc tgttctgccc catcaccagc cagatcaaag
    12121 gctatccttt tgaagtcatc atcccaaagg attgcagagt atcgggagcc gtcctctcgg
    12181 atcagatcaa gagcctggac tggaaagccc ggcgggcctc gctgatctgt tccctgcccg
    12241 aatccatcgt ccggcaggtg ctgaacaagg tcggaaccct gctggatgcg tgatgggtga
    12301 ttccggctgc ggccatcaat tctggagggc ggaactcagt aacgccgtaa gaagatggtc
    12361 gcgacggagc gcgaccctcc aaaagttgct ggagggtttc gctccgtcaa aaccgcttgt
    12421 cgttaaggta gggcggtcgc tccgcgaccg ccgcggcgcg cacggagtgc acgccctacc
    12481 atcgccaatg ggattacttc gcagcgggcg aggctaaagt gccgggggct tccggcgcag
    12541 cgatacggtc agcagtgaac ccaggccgag caggacaagc gtgaacggct ccgggatggg
    12601 tgtcacattg agagtgccat aaatcccctg aacatcagag agattgtaat acggcagatc
    12661 atatgttgtg tcctgcgcca gatcccaaat cccggattga tagctggacc cgttcgtcga
    12721 gcgcgggatc aatgccccgt ccatttccgg ctgcgagcgg atcgcgaaat ccctcggcct
    12781 tccaatcaag gatctttttg atttctgagt tgaagaaggg gtttaggctg tagtccttta
    12841 gactgttagt ccgatttctt tcacggcgag ttattccgcc cgctcttcga gattcttgcc
    12901 cttgggcgct ttcttctggg gaatgacgag gccctgcttt tccgcctctt ccgcgaagct
    12961 tggaatctgg tcgcggtagc gcacaaccat ttttttcagg gccatgtgct gcttgaatga
    13021 gagctcgtgc ttctgcgcat actgcgcctt cagcgactgg tagaaggcat ggtcgtccca
    13081 ttcgcggcgg ccgcgcttca ccggctcccg ccaggcctga accgattcca tcagcgccag
    13141 aatcgggccg ctgtcttcgc caggcttttt ctcctgctcc acaagcccga actgcgcaac
    13201 cacctgctcg cggccgccga tctggtcggc gtatttcagc agcatcttgt ccagagccgc
    13261 cacctgacgg tcggaaaatc ttcgtccctc gtcattctgc ttcttcaggg attcatagaa
    13321 aacccggtca tcataggttt tcttgccgat cgtgcgcgca tccttgaacg ccacatccgc
    13381 cagcgcatcc agtttcccgg aggtctcctc gcgcggcggg agattcttcg cctcgatggc
    13441 tccgatggcc tccccaaggc cctgcgccac gacgaattca tcccagccgg atatctggtc
    13501 cttgtatttc acacccagtt tcagcagcgc atccgcctgc cgctgcgaca cggccttttc
    13561 gccggcttcg aactgctggc ggacggactc aacaaacttc tcatcgctgt attcccgctt
    13621 gcccgcctga cgcccgggcg cccattcgcg gacatgctcc agcatgccca gaacctgccc
    13681 cacatgcccc ggatcggccg aaggtccctt ggcctgctga acccagccga gaaagttctg
    13741 atagaattca cccagcatct gatgccattc gacttcgccg ctctcaatat catcgagctt
    13801 cttttccatc gcagccgtga actggacatc aaagagctct gacaggtggg cgacaaggaa
    13861 atcgcagacc cggtcgccca gttcggtcgg caggatggct ttcttttcgc gggcgatgta
    13921 cttgcgcgca ttcagggtgg acagaatcgc cgcgtaggtg ctgggccgcc ccaccccgtt
    13981 ttcctccagc gcgcggatca gagaagcttc ggtgtagcgc ggcggcggct gggtttcctt
    14041 ttccgccctg accggctcga cacattccag gccctcacct tccgtcagcg gcggcaggct
    14101 gccctgggta tcgtcctctt cctcctcgcc ttcgttctgc gtctttgtct tctcgaggcc
    14161 cgaagccttc atataacccg ggaagagaac ctgtgacgca ttggcgcgga aaagcagcgg
    14221 atccgcgccc tcgcgcttcg gcagcgcctc gatctcaacg ctgcgctgcg cgatgcgcgc
    14281 gggggtcatc tggctggcca tgaaacgctt ccagatcagc gcgtagagtt tggcttcctc
    14341 aggcgaaagg gcagactgca ccgattccgg agtgcgtgac ggatcggtgg gacgaatggc
    14401 ctcatgggcc tcctgcgcgg tgccgcggct tttgtagaaa ttcggcttct caggcacata
    14461 gtcatcaccg aagtttttac ggataaaatc gcgcgcctgg tcctgcgcga ccttcgacac
    14521 gttgaatgag tccgttcgca tgtaggtgat cagaccgatc cgaccctcgc cgaggtccac
    14581 gccctcataa agcttctgcg cgatcatcat tgtgcgcgac ggggaaaatc cgtggacgct
    14641 ggagccggcc tgctgaaggc tgctcgtgat gtagggcgga cgggcgcgct tctgcgtttc
    14701 cttgtccttg atgctctgca cgcgaaacgc gctgttctga acgtcttcaa aaatcctttc
    14761 cgcctgcgcc tcatcgcgaa gatcggcttt ttcgtttccg attttcacga ggcgcgtctc
    14821 gaacacatcg cgcggatcca ccagcttgcg cagctttgcg cccagcaccc agaacttctc
    14881 cttcacgaaa ttccggattt ccttctcgcg ctcgcacacc agacggaggg ccaccgactg
    14941 cacgcggccg gcgctggcgc cgccgcggac gcgcgaccag agcagcgggc tgaccttgta
    15001 gcccacgatg cggtcgagaa tgcgccgcgc ctgctgggag ttcacgcggt tctggtctat
    15061 ctgccgggga tgcgcaaacg cctcgcggat cgcgggcgcg gtgatttcgt tgtaggtcac
    15121 gcgcagaaag ttttcagccg gaacctgctt ttcaagcgcg gcctgcagat gccatgcgat
    15181 cgcctcgccc tcgcggtcgg ggtcgggagc gagatagacg gtttccacat ccttcgccgc
    15241 attctgaagc tccttgatga ccttctgctt gtccttgatc accttgtact gcggcgtgaa
    15301 gttcttctcg atatccacgc cgagctgctt ttcgggcaga tcgcgcacat ggcccatcga
    15361 ggccttgacc agaaaatcct ttcccagaat cttgttgatc gtgcgcgcct tcgcaggcga
    15421 ttcgactatg accagcttct tgcccatgtt gtgctccctg aaaaaccgtt tcggaaaaag
    15481 ctgtggtaaa tattccaatg gctggagctt ggcaacctat tttttccaat ggctggaagt
    15541 ttctgcgaaa aaagttccaa ccattggaag tttttttgcg gaaagttcca atgattggaa
    15601 cttttcggaa gttgagccgc cttgcataaa ttatttcatc tgctaaagtc ggccgtctcg
    15661 ttgattgcag ttttaagaaa ccatgaagac actgatgctg ggaaatgaag cgattgcgcg
    15721 cggcgcctgg gaagcgggcg ttcgcgtggc cacggcctat cccggcaccc cgagcaccga
    15781 agtcaccgaa tatatcgcgc agtacaagga gattgacgcc gagtggtctg tgaacgaaaa
    15841 agtcgcgctc gaagttgcca tcggggcctc cgtggcgggg gcgcgcgccg tatgcaccat
    15901 gaagcacgtc ggcctcaacg tggccatgga tcccctgctc tcaatttcct atcccggtgt
    15961 caacgccggg ctggtggttg tcgtcgccga cgatcccggc cagcacagct cgcagaacga
    16021 gcaggacacg cgctacttcg gcatggccgg aaagctgccc gtgctggaac cgtccgacag
    16081 ccgcgagtgc cgcgatttca tgatccgcgc gtttgaaatc agcgaggagt tcgacacccc
    16141 cgtcatcgtc cgcaccacca cgcgcgtggc gcatgcctac gggctgctgg agccgggcgg
    16201 gcggatggat gtgccgcttc gcgaatacga cagccgcttc gggaaataca tggtcgcgcc
    16261 ggccttctcg cgcaggcgcc gcgtggtggc cgaggagcgc ctcggaaaac tccgcgagtt
    16321 cgccgaaacc accgatctga atatttacga aaatggttca tcagacacct gcatcatcac
    16381 cgggagcatc gcatatcagt actgccggga agtcatgccg aatgcggggt tctgcaaact
    16441 cggactgtcc tatcccctgc cggaaaggaa actgaaggac ttctgcgggc aacataaaaa
    16501 ggttttcgtc gtcgaggagc ttgagccgat ttttgaacag cacctgaagg cgcttggagt
    16561 tccagtcatt ggaaaagcaa aagttccagt cattggagaa ttgagcacgg agattgtccg
    16621 caaggggttg acggataccg cgcccgaacc ggtttcaacc ccgttcgaaa acctgccggt
    16681 gcgaccgcca gtgctctgtc ccggatgcca tcaccgcggc cccttctatg tgatgaagaa
    16741 gctcaaactc aaggtggccg gcgacattgg atgctatacg ctgaccgcct cgccgccgct
    16801 gagcatcatg gacaccgcga tctgcatggg cgcgggcatc ggcatggcct tcggcatgca
    16861 gaaggcgcgg ggaaaggatt ttgcgggaaa atccatcgcc gtgatcggcg attccacctt
    16921 ctggcattcc ggcatgaccg ggctggtgga tattgtctac aatcggggca tcacaaccgt
    16981 tgtcattctc gacaacagca tcaccgccat gaccgggcac caggagaatc cgtccagcgg
    17041 cagaaccctg aacggcgaat ccgcaccggc attgagtttt gctgatttcg caaagtccat
    17101 cgggatcaag cacgtccgcg aagtggacgc tttcaatttg aaagagctcg aaaccgtgat
    17161 gcgcgaggag ctcgcgcgcg aagagccgtc ggtgatcatc accaaacgca cctgcatcct
    17221 gcatccgtgc gccgtccggc atttcccgct gaaggtgaat cccggtctgt gcaagtcctg
    17281 catgaaatgc ctgtccatcg gatgcccggc gatttccttc gacggagaca aggtaaagat
    17341 tgaccaggtg ctctgcgtgg gatgcgaact ttgcgcgggg atctgcccgt tccatgcgat
    17401 cgagaagcag ccccggcccg gcggaggacc ggcatgaaag ataccaccaa catcctgatc
    17461 gcggccgtcg gcggacaggg cgcgctgctg gcctcgaaga tcctcggggc cttcgccacc
    17521 gcgcgcggag atgacgtgaa gatgtcggag attcacggca tggcccagcg cggcggaagc
    17581 gtggtgaccc atgtgcgttt cggggagaag gtgttttcgc ccaccatcga gcagggatcc
    17641 gccgacgttc tgctggcctt tgaaatgctg gaggccgcgc gccatgccgc catgctgaag
    17701 cccggcggca ccgtgattgt gaacacgcag accatcgcgc cgcttccggt cctcacgaat
    17761 gaaatgccct accccgccga catcatcgga aacctgaaga agatgaacct gcgcgtgtgg
    17821 cacacggatg ctcttgacct ggcgcgccgc gccggacatg tcaagacggt caatacggtg
    17881 ctgatcggca tgtatgcccg gatgacggga gaccccgccg aatcctggct caaggtgctt
    17941 gccgaaaacc ttcccccaaa gctttataaa gtgaatcagg ccgccttcac ggcgggatat
    18001 aatttgagcc ctccgtggac agagcaggag taaacaatga tctggaatga aaaatgcgag
    18061 tgcatgccgc gtgatgaact gaccgccctc cagaccaggc gccttcaagc aaccgtaaaa
    18121 cgggtttatg aaaaggtgcc cttctaccgc cgcaagatgg atgaacttgg ggtcaagccg
    18181 caggacataa aaagcatcag ggatatccgg catctgccga tgaccaccaa gcaggatctg
    18241 cgggacaatt atcctttcgg actcttctcc tcgcccatgt ctgaaatcgt gcggctccac
    18301 gcctcatcgg gaacgaccgg caagccgacg gtggtcggct acgcgcgcga ggacattgaa
    18361 ctctgggcgg atgtgatggc gcgcacgctc tgcagcgcgg gcgggaccga gcacgacatc
    18421 atccagatat cctacggata cggactcttc accggcggcc tcggggtgca ttacggcgcg
    18481 gaaaaactag gggccacagc gattccggtc tcaggcggca acacgcagcg gcagatccag
    18541 attctccagg atttcggccc gactctgctg gcctgcactc cgtcctattc ccttttcctc
    18601 gcggaagccc tcgcggagcg cggcattcat cccggtgatt tgaaactcaa ggcggcggtg
    18661 ctgggcgcgg aaccatggac cgaaaacatg cgcgcggcca tcgaaaagaa tctcggaatc
    18721 agggccattg acatttacgg cctcagcgag atcatcggcc ccggcgtcgc cagcgagtgc
    18781 tgggagcaga agggacttca tatcaacgaa gatcatttct atccggaaat cattgatccg
    18841 gtcacgctgg aggtgctgga gccgggacag cacggggagc tggtcttcac cacgatcacc
    18901 aagcagggca tgccgctcct gcgctaccgc acgcgcgacc tcaccagcct gatcacgggc
    18961 gcctgcccct gcgggcgcac caccgtgcgc atgcacaagt gccagggccg gagcgacgac
    19021 atgctgatca ttcgcggggt gaacgtcttc ccctcgcaga tcgaaagcgt gctcctggag
    19081 atgtccgaaa cgcatccgca ctacctgctc attatagacc gcgtgaacaa cctcgaccag
    19141 ctcacgcttc aggtggaggt ggacgcgcag ttcttctcgg acgaaatcaa gcagctcgag
    19201 gcgctcagca ggcgcatcaa gcacagtgtg gaaagcacgc tggggctttc ggtcaatgta
    19261 cggctggtgg aaccgcagac catcgagcgc agcgagggca aggcccggcg agtgatagac
    19321 aaccgcaaac tttcataagg aggattcgcc atgattattc agcaggtttc cgtttttctc
    19381 gagaacaagt ccggccggct ggtggaagtg acacaggcgc tggcggatgc aggcgtgaac
    19441 atttccgcgc tgagcattgc ggaaacatcc gactacggcg ttctgcgctg cgtggtatcg
    19501 catccgcaga aggcggtgga actgctgaga gcgcggcact tttcggtcag cctgacggat
    19561 gtggtctgca tggccgcgcc gaatcagccg ggggcgctga accgggcgct gaagattctg
    19621 gccgaggccg gcatcagcgt ggaatacatg tacgccttcg ccattggcga ccgcgcgctg
    19681 gtcgtcatcc gcaccgacaa catcaaggac accatcagcg ctctccagca tcacaaggtg
    19741 gacctgctga agtcgagcga actgtatcag gtgtgatcag accgtctttc tgctccaggt
    19801 gccgcgctca aaccaccatg cggcgagcag gccctggaag ataatggccg ccacgatggc
    19861 ccaccagatg ccgctggtgg cgggctccca tatcctcgac agcatgaccg cgaggggcac
    19921 ctgcaggccc cagagggtcg caacggtgat gatcatcggg ctcatgctgt cgccggcgcc
    19981 gttgagcgcc cggccgaaca caatgcccag cgccgcgaag atgtacgaag gggaaacgat
    20041 gcgcagatag tccgtcccgg ttcggaccac atccggctcg ctgttgaaaa accggatcag
    20101 ggccggcgcg aagatcatca gcaccgcggc gatgcccgtc atgaacacca tatccagtcc
    20161 cacagccgcc caggcggcgc gacgggcgcg ggccggcttg cccgcgccga ggttctggcc
    20221 gaccatggtg gcagccgcgc cgcccagcgc gaaggcgggc atcagggcga tcatgtggaa
    20281 ccgcatgccg accccgtagg ccgcaaccgc ggcggtgccg caggacgcca caatgcgcat
    20341 caggaccgcg ctcatgaggc tgcgtgaaag catctggccc gagccgggaa gcccgattct
    20401 gagaatccgc cacgagagct ccgcgcgggg gatccactgc ttcagatgaa tccgcagatg
    20461 agttttcccg gtaaaaagga tatagagcga aagccctgcg gcggcggcct gagaaatcac
    20521 ggtggcgctg gcggctccgc ggatgcccag acagggaaaa gggcccggac cgaaaatcag
    20581 gagcggatcc aggatgatgt tcaccacatt cgaaaccgcc atgatgcaca tcggagtgac
    20641 tgcatccccg gccccctgca gggccgcatt gccgataaaa agaataaaca ccgtgaaact
    20701 gccgatcagg catattcgaa gatagatgct tccgtcatgg atgagctccg gctccgcccc
    20761 gagcaggcgg aacagatgag gcgaaaacag ccagccgcag gccgccgtga tgccgccaag
    20821 cacaaccgcc agcatcaggg actgcccggc ggcggccgcg gcatcctccg gccggcccgc
    20881 ccccacggag cgcgatacca gcgcgatggt tcccgtggaa agacccatca gcatggggaa
    20941 gagcaccatc aggatggttc cgctcatcgc caccgcggcc acggcccggg atccaagacg
    21001 ccccacccag aagaggtcaa tcaggctctg cacattctgc agcaccgcac cggccagcat
    21061 cggcacggcc agcacagcga tggctttgag caaaccgccc tccgtgagca cttccctgtt
    21121 tcttctggcc gcaaatattt tcatgatcgg aaaatcacct tgcgtgattc tggatatctt
    21181 tgcgccgaag tcaattgccg acggtaaaaa gctttccgcc tgaaaccgcg gcagttcgaa
    21241 ctccgatcac tggtgcaaaa agcgctgccg tgcggattcg ggaaatcttt cgatggcgta
    21301 acgcagcatc gttcgaggca tgctgcgcgc atgccgctcc agaaactccg tctcgatgct
    21361 ccggtcccgt ttgccgacct cgcgaagcat ccagcccgcc gccttgtgga tgagatcttc
    21421 ccggtccggc agcagcattt ccgccagttt caaaaggtct tcgaaatcgt tccggcggat
    21481 gaagtggaac gtggccagga gggcgatccg gcgctcccag agactttcgg atttggccag
    21541 gcggtacagc ggcgcgcgag aacgggtgaa caaatgagcg ccgacaacat gctccgcgga
    21601 acagtccaca agatcccagt tgttaatgca ggccgtatga tgcagataaa cccggtacag
    21661 ggcgttccgg gcctgttccg agcctgccgc gtactgtttc accatgatca gcagggccag
    21721 caggcgcgcc tcatgaaatg gcgattgcag aatctcgagc accacatcaa gcgggagatc
    21781 gcgccactcc ttcacgcact gtctgatgac cggaacacgg atgccgagga acaggtcccc
    21841 ttccccgtat tcccccttgc ctgtcttgaa aaaacgctgc gagtgccggg cgatttcatc
    21901 gctgcccagc gctttcagcc gcagaatgat ctgccgggat gaagtcatca cagaagcatc
    21961 ccggcaatac aggcggtcat aaaggcggcg agcgatccgg ccaccatggc cttgaggccc
    22021 agctttgcga aatccctgcg gcggctgggc acgagcgagc caatgccgcc gatctggatg
    22081 gccacggagc tgaaattggc aaatccgcag agcgcatagg tcatcagggt gaaggtgcgc
    22141 ggagaaacct gatcgcgcca cttgatgagg tccacatagg ccacaaactc gttgagaacg
    22201 gttttttcac ccagcagggt gcccgcgatg ccggcatcct gccactcgac acccatcatc
    22261 agcgcgaacg gcgccatcat ccagctgaac acgcgctgga gggtgagcgg ggcgccgccg
    22321 atatccggga gcagcgtgca aagccagttg aacgcgccca cgagggcgat gaaggcaatc
    22381 agcatggccg ccacattaag cgccagcatc aggccctcgg acgcgccccg gcaggcggca
    22441 tccagcacat tcgaatcctg tttcggcacc tgaattctca catagcccct ggtattggac
    22501 tcctcggttt ccggaacgat gatcttggcc agcacgagcg aggccggcgc ggacatcacc
    22561 gaggcggtca gcagatgccc cgggtccgcc cccatgccgg cgtaggccgc cagcacaccg
    22621 cccgccacgg tggccatgcc tccggtcatg agcgccatca gctcggaacg cgtcatcgtc
    22681 agcagatagg gtttaacaac caggggcgcc tcagtctgac cgataaagac attggccgcc
    22741 gccgccagcg actctgatcc ggacgcatcc atgaccttca ccatgaccct tgccataaaa
    22801 ctgaccacgt gctgcatgat gcccagatgg aacagaatcg ccatcagtga cgacacgaaa
    22861 atgatggtgg ggagcacgga gaatgcgaaa tagtgctcgc ggaactgttc accgaaaaca
    22921 aactgtgcgc catcgtcgga gaaactgatc aggcgcgaga tcaccgcgcg ggccacatcg
    22981 aatattttca gccccacgga cgtcttcatg ataaacacgc cgaaaagaat ctgaaggccg
    23041 accccggaga ggatgaggcg gaaatccatt cgacgccggt cggaggacag cagccacgcc
    23101 agaagcatca tcaggatcag tcccagaaag ctgttcacgc ggtagatcat ttgacagtct
    23161 ccttatacca gcctcaaaag ccggaaaata gccccgcgcc cgcaatatca caagcaggat
    23221 tcctgcccgc gcgcggttta cgcttcctat cctgcggccc aaccgctaat aattctgcac
    23281 agtgaaaggg gtgtgaaatg gagctgctga gttttgtact gctggtgctg atgatcgtgg
    23341 tgctcgccaa actttccagt atccggggag cgctgaaaaa catcgagtcc gagcagtcca
    23401 atctccggcg gatggtgaaa aaacttctgc ccgcggaaga gccgtccaag ccatggaccc
    23461 cgccgccccg gcaggaaatc ccggctcctg tgccgtcccc cgcccctgcc gtcccggagc
    23521 ccccccgtgt tgccgccgca ccccagactc cgccggcggt cccgtccatg ccggcctatc
    23581 cgcgcatcac gcggcagccg tcgcgtgtcg gggaaatttt gagaaaaatc tggagctgga
    23641 ttcttgtggg cgaggaacac cggcgcgagg gggtcagcgc cgagttcgcc atcgccagca
    23701 cctggctgct gcgcgcaggc attgtggcga tcgtgacctg tgtcggattc tttctgaaat
    23761 ggtccatcga gcggcagctg ctcgggccga cgggccgcac agccatcgcc attgccgccg
    23821 gcatcggcat gctggccgcc ggctaccgcc tgttcgggaa gaaatatcac ctgatgggcc
    23881 aggggctttt cggcggcggc ctcgccacgc tctatttcag catgtatgcc gcagggcctc
    23941 tttacaaact gctgccgatt ccggccgcct tccttctgat gattctcatc accataacgg
    24001 ccggcctgct ctccgtgcgg gtgaattccc tgcttgtggc cattctggga atcattggcg
    24061 gcttctgcac gccgatcatg ctcagcacgg gcaagcccga cttcatggtg ctctatgttt
    24121 acatgctcat cctcggagcg ggcgtttttg ccctttcgca ggtgaaatcg tggcggctgc
    24181 tcaactacct gagcttcttt ttcacctgga tgctggttct gggatcactg ttcagctatc
    24241 gcaaacccga cttcaatctt gtcatgcccc tgctttctct gctgttcgcg ctgcatgcgg
    24301 ccatcgtgat ctggcacaac atcggccgca acgggaagct gacggtgctg gaaattattt
    24361 atctgctgat caattccgga ctgttctccc tgacggccta cagcctgatc cggtacaccc
    24421 acgggcggcc gtatcccgcg atcctcacgc tggcgctggc ggtgttttac atcgggcacg
    24481 tgctggcctt cctgcagttc aagcgcggcg accgcgccct gctgatcacg atgatggccc
    24541 ttgcgggatt ctacacaacg ctgacgatgc cgctggtgct ggagcgggag tcgctgacaa
    24601 tgagctggtc gctgctggcg ctgatgttcc tgtggcttag cggaaaggtg cagagccgct
    24661 tcctgaaccg gctttcctac ctgctgtatg gcctcgtgtt tttcaagctc gccgcggtgg
    24721 acctgcacga gaacttcccc cacccgtcca tggggccggg cggaacgttc aagctctatc
    24781 tcaaaagcat gacggaccgc ctgtggacct tcggcgtcag catcgcatcg gtcttcggcg
    24841 cgttctggct ggaacgccgg cggccgcagc cggggccgga ggccggcccg gaggaaactc
    24901 cttcgcgcgg gctgctcccg acgctcttct actggatcgg tctttcggtt ctgctgatct
    24961 atctgctctt tgaaaccaat gcgttcttcc ggttctatgc gccgtggcgc ctgccgatga
    25021 tgacagtggt gttctgcgtg atggggctct acaccctctg gcgcttcttc gccacggatg
    25081 accggtcgca cctgatcgcg tcgaacatcc tgctgggcct tgcggccgca aagcttcttt
    25141 tgtgggatat tccgcactgg gatatttcgc gcggcggact gtatggctgc gaatattctt
    25201 ccgtgatggt gctgtcgcgc ctgctggatt tcggcctgct gctggtgatt ctcttcggcg
    25261 tgtgggccct gtttgtccgg cgggacagcc ggaaatcgct ggcgccgttt tacggctatg
    25321 gaggactggc gcttctgttc ctttacacca cgcttgagct gaatgcgttc ctgcacttca
    25381 aggagccgtc attccgcgcc ggcggcgtgt cggtgctctg ggccatctat gccgtctgct
    25441 ttgtcgcggg cggcatctgg aaaaagctgc gcgggctgcg gctggccggg ctgctgctct
    25501 tcgccgtcgt cacggccaag gtgttcctgc acgacctcgc gggcatgccc gccatctggc
    25561 gtgtggccgc cttctttgtg gtcgggatca ccctcctgat cggggccttc gcctatctga
    25621 atgccgcgcc gggtttcatc cagaaaaagg acgagtgaat catgaaaaga ctgcttgccg
    25681 tttccatttt cactttttcc ctggcgcatt gcgcggacgc ccgcatcagc ctgcgcgatt
    25741 atccgtggat gaagcccctg atccgtcccg acggaccgca ggccgaggtg gcctcgttca
    25801 cgctcgacaa cccgatgtat gccgccacgg atgaccagta tgccaatatc cgcatcacgg
    25861 atgaaaacgg ggccgaagtt ccctacggcc tccggaagaa ggtctatgcc gtcacgcaga
    25921 tcgtcgagcg ggccaccgcc tcggagattg tttcgtttga ccggcaggca aacaacagcg
    25981 tgaacgtggt gctcaggaag aaggacgcga aggcccggcc ggaggcggtc atcattcaca
    26041 cccgccaccg gaacttcgaa aagcccgtgc gtatattcgg aagccgcgac ggggcatcat
    26101 gggaactgct gaaggaatcc aggatctacg actacagccg ccacattgat gtccgcagcc
    26161 tgcgcacgga tttcccgccg tcggatcaca ccctctttcg cattgaaatc gggaacttca
    26221 tcgagcaggt gcccgacgcc acaaccggca tcagcaccga gcggcgcggc gacgcggtgt
    26281 tcagcgagat tgagcaccgc gccctgcagg ccgagaccat tcggattgaa tccgtcgaac
    26341 tgcgcacaaa ggagcgcgtg atccggcccg acagcgcgca cgtcaaggtg tacgagagcc
    26401 gcgacctgcg gatgagcagc agcgaggaaa agcagaccac ctcgtggacc ttcggaaccg
    26461 accgccagcc gctgacctcg ctcagctttg aatcggccag ccgcaacttc agccgcccgt
    26521 tcaccgtgga ggggcggcgc ggcagccggg aggaggcatg gcgcacactc ggatcggcca
    26581 cgctgcaccg gtttgaactc ggcggcatcc gggatgagaa cctgaccgtg atgctcggcg
    26641 gcgactgccg ctatgacgaa tatcgcgtga ccgtcttcaa tcacgacaac gcttcgctcg
    26701 acatctccgg cgtggtggcg acgggatatg tctatgaagt gcttttcctc cccgatccgg
    26761 gggcggacta caccgtgctg tacggcggaa gcggcatccc gcggccgcgc tacgatgtgg
    26821 agaccgtgct gagccgcgtg cccaccattg aatccaccgt catgagcctc ggcgagcaga
    26881 aggcgaaccc gaaatacaag ccgggcccgc gctgggacgg gaaaacgtcc aaaatccttt
    26941 tcatcgcggc cgtcttcggc atggtcatcc tgctgggctg gttcatcgcc aaaggcgcgg
    27001 gcaaaatcga ggccatggaa gagtaataat tacggctcat cgccgggcat gcggttttga
    27061 gcgccgaaca acccgttttc aggcatttat tcaaaaaaac gcgatttttg agcgttttta
    27121 agcgtttttg ggccattttt ggcctgtttt tggacttttt tgagcatttt cgaggccccg
    27181 tttaatatag tactatttta gtactatata agcagaaagg ccggtttctg atactgcttg
    27241 atttctggag gcccggacgg cgctccgcgc ggtttttcgg cttccggtat tgaagattcc
    27301 cctttgatgt gtagtatcga tgcgaatcat ttttttacgg aggcagcagc atgcggattt
    27361 tagtgacagg cggaacaggt ttcacgggca aggcgctggt caggcggctt ctgaacgatg
    27421 gccacagcgt gattgccatg gactacaagg aaggcatcaa gacccaggag ctgcgcgact
    27481 ggggcgcaga agtcgtgatc ggcagcgtga ccgaccgcga ggtggtcaag cagtgcatga
    27541 agggcgtcga ggtcgttcag cacgttgccg ccgcgttccg cgaaatgaat gtccccgagt
    27601 cctactacga cgaggtcaac gtcggcggca cgcgcatctg ccttgaggaa gccatggccg
    27661 ccggcgtgaa gaagttcatc tactgctcca cctgcggcgt gcacggcaat gtgaagaatc
    27721 cccccaccga cgaggcccac aacatcgccc cggccgacta ctaccagcag accaagtaca
    27781 acgccgagcc ggttgtgaag agcttccacg aaaagggcct gccctgcgtg atcctgcgcc
    27841 cctccgccat ttacggcccc ggcgaccccg agcgcttttt catgatctac cgccgcgccg
    27901 ccaag
//
LOCUS       DAOQWR010000016        27299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2047_length_27299_cov_5.369733, whole genome shotgun sequence.
ACCESSION   DAOQWR010000016 DAOQWR010000000
VERSION     DAOQWR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 27299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27299
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_27299_cov_5.369733"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..816
                     /locus_tag="PKK36_02505"
     CDS             82..816
                     /locus_tag="PKK36_02505"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNR93459.1"
                     /translation="MKAEQTAPRFRDLKIKIFSDGADLDSIIEAYQKGDVSGFTTNPT
                     LMSRAGITNYMTFATEVLRVVTDLPVSFEVFSDDFAEMKEQALKLADLAENVYVKIPV
                     TNTKGKSACPLIKDLAGRGLKLNVTAILTLKQVEEVAAALNPKVPAIVSVFAGRIADT
                     GRDPVPYMKESLKILKPLPLAELLWASPREILNLVQADEIGCHIITVTPAVLSKAGGL
                     GRDLAELSLDTVRMFYNDAQGSGFTI"
     gene            830..1840
                     /locus_tag="PKK36_02510"
     CDS             830..1840
                     /locus_tag="PKK36_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93460.1"
                     /translation="MIITRTPFRITLGGGGTDLPSFYEEHGGYVFSMCINKYMYVMLN
                     RRSVADRKIVIRYSDVETVNTVDEIRHPLAREALRMHGVHENIELTSIADMPAKTGLG
                     SSGSYLVGLLTAIHAYKREPVAMEQIAKEACRIEMDILKEPVGKQDQYMAALGGFQAL
                     EIDRDGTVRAESVPVDFMTVSELVLKARIYYTGIQRSATAVLKNQDEAARRKERADHK
                     QVVDSLCRIKEIGKGIKKAFDDCDLDAFGRMMDEHWQHKKSMSKSISLSVLDELYDEV
                     KLRFGVLGGKIIGAGGGGFFMLYCPGKGRELDEYMASKGMPRINCMPAREGAKVVTDL
                     FE"
     gene            complement(1850..3274)
                     /locus_tag="PKK36_02515"
     CDS             complement(1850..3274)
                     /locus_tag="PKK36_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93461.1"
                     /translation="MHVVLIQPPEGTAGAAPLRLARKNPATFAPDWDVLCLQAYLKGH
                     SRHLSTCVDCRLFTDLELELEHALNAVKGPKIIAVHTSLTNIGESAAVLELSKRRWPE
                     IRTVMFGQFPSQFPEHIHAVARADYALSGDPEPILRALLDHIDSEKRLQNVPGLNFPG
                     KNISTSWMPDLNILSLPDWGRVFWRAYHHPDQAGRQPARMRLSRGHTQEACDRAMGLI
                     REPLRLWPMDKLAQSVRASSDLGVTDIFLTDPPGVWNEGRLREWCRALNRIYNKQPWG
                     LQLNPGRIEDDLIAELAATRCRRVDFIVPSCDQELQERFGVPFRARTLRHTISSLEKA
                     GVEVFVRLWIGGPEEKKGAVRRAVALIHHLPAAHIVLEPFPFCMDSPLYRSAAADCGA
                     SDIDNWLRWTRDPWILDRPEAVWNARAGAEALQKQITGIYRRVVRSPRWQSRRLGRLL
                     KSSYAVQTIREGADWIAGRLPRRA"
     gene            3368..4987
                     /gene="nadB"
                     /locus_tag="PKK36_02520"
     CDS             3368..4987
                     /gene="nadB"
                     /locus_tag="PKK36_02520"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNR93462.1"
                     /translation="MKIIDCDVLIIGSGLAGLTAAWHLRDAGRIAVVCKRDPMESNSN
                     RAQGGVACAIDSDDSVEEHVRDTLGTGGGLSREARVRDILSAGQAGIRELEKIGVHFS
                     RREGAADYDLGQEGGHSHRRVLHAGDITGHEIMRVMTERAAAQSGITIYPETMAIDLV
                     STAWLRRRGDNRCVGAYFLDCKTEKILAFRAPHTVLAAGGTGKVYLYTTNSDVATGDG
                     VAMAWRAGIPIQNMEFIQFHPTCLYHPAAKSFLISEAVRGEGAELINARGEAFMKRYD
                     PRGPLAPRDIVARAIDHEMKISGDACVYLDITCKGEEFLRRRFPNIYATCLNYGFDMA
                     RAPVPVVPAAHYCCGGVEAGVDGRTRMKGLYVIGETACTGLHGANRLASNSLLEAMVC
                     GRRAAEAICAAPAEAVAGIKIPDWQYGNAVPSDEAVVVEHNWNEVRQCMWDYVGIVRT
                     TKRLERAARRIDNLQREIREYYLNYFVTRDVLELRNIAAVAGLIIRCAQRRRESRGLH
                     YTLDYPGLDESRDPQDTIIADKPGGSIATVE"
     gene            5028..6116
                     /gene="aroB"
                     /locus_tag="PKK36_02525"
     CDS             5028..6116
                     /gene="aroB"
                     /locus_tag="PKK36_02525"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNR93463.1"
                     /translation="MSKLIRVELGDRSYNIHVAPGLLAQCGQFCRDLKLGHRCLMISD
                     ANVAPLYGHIVRESLLEAGFMPHVFVVPAGETSKSQATLSLAYDEALLHGMDRKSFVV
                     ALGGGVVGDLAGYAAATFLRGIPFVQIPTTLMAMVDSSVGGKTGINLPQGKNLIGAFH
                     QPSAVIADLSAFTTLPDREYISGLAEVVKYGVIQDADFFASLEKNADRILAREPETLA
                     QVVARCCECKAEVVRLDERESGLRAILNFGHTLAHAIEQAAGYGKFLHGEAVAIGMAY
                     AAALSVSVAGLSNEAAERIIRLLEKFGLPAAVSGLNWSAVRRAMKTDKKNAGGTVRFV
                     LAREIGRAEPGIEVSEELLEEVWNVIGQ"
     gene            6100..6702
                     /locus_tag="PKK36_02530"
     CDS             6100..6702
                     /locus_tag="PKK36_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93464.1"
                     /translation="MSSVSEWVVREYFEALGFLVQQPVKYQVAARRKRAEEEIDLLVM
                     NPLGGDHALPSKILWKSPDLRNVTRAVVSVRGWHTDKFSPKTLELSPEVFRFAEDAAM
                     RKASGVLGEGPVARILCLPDLTSTESLRKRTLEMLRGNGIDGVLLFRTMLLELVAHVE
                     TQKSYEKSDLLQILRLMKNYDLIKDPQMDLFASKRRKRKA"
     gene            6704..7822
                     /gene="dprA"
                     /locus_tag="PKK36_02535"
     CDS             6704..7822
                     /gene="dprA"
                     /locus_tag="PKK36_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR93465.1"
                     /translation="MTEREAYIALNMLDGMGPVRARNLIERLGSPEAVFDAEPEALME
                     AKGIGQEMAESIVNQRDEINPAEEIEKTEALGGRILTCLDDEYPAALRTIYDPPLALY
                     ILGKLVPEDKHAIALVGSRRSSHYGQQTADRLAFQLAKVGYTVISGLARGIDTEAHKG
                     SLKAGGRTIAVLGGALDKIYPPENAGLAEQIAQHGAVISEFTLGREPDKTTFPYRNRV
                     VSGMSMGVVVVEAPFKSGSLITAEQAMDQGRSVFAVPGRIDNPNARGCHMLIKSGARL
                     VENVDDILQEFEMLIPQEQKDVAKAASRRPEVMLTDDEEKIVRALGSDTLDIDTLARN
                     AELNIASVSGLLIGLELKKVIRMLPGRLVELIARAEDF"
     gene            complement(8110..9132)
                     /gene="rlmN"
                     /locus_tag="PKK36_02540"
     CDS             complement(8110..9132)
                     /gene="rlmN"
                     /locus_tag="PKK36_02540"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNR93466.1"
                     /translation="MITLLGQTQAELVALAATLGQPAFRGRQICEWIYKHHAETIDAM
                     SSLSKSLREQMAADCTTGRSAPVECAESADGTKKYLFPAGEGKFIETALIPDEERATL
                     CISTQVGCARACAFCATGQQGLQGNLSAAEILNQYASCPERDQITNVVYMGMGEPLDN
                     LEAVLRSVEIFTADYGYAKSPTRLTVSTVGILPALETLIKSCSCHVAISMHSPFGEER
                     AAMMPVEKQHPIKEVVEVLRRAAIRGQRRLTFEYALFDSVNDSPAHAKAVARLLKYLD
                     CRINLIPFNAVPGSSFVPSSREKVEIFQNILKDAGFVTTIRKSKGQDIAAACGMLSTA
                     RLKGLG"
     gene            complement(9129..9401)
                     /locus_tag="PKK36_02545"
     CDS             complement(9129..9401)
                     /locus_tag="PKK36_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93467.1"
                     /translation="MSPFEIAMLLLFGASWPVSIAKALRTKVVAGKSPVFMVIVIIGY
                     LCGVLHKAFYHFDPVIFLYALNCGMVCLDLFLYLRYLPLQEKLIRQ"
     gene            9587..11191
                     /locus_tag="PKK36_02550"
     CDS             9587..11191
                     /locus_tag="PKK36_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR93468.1"
                     /translation="MRCPGYIFVSLITTGILTGCGTFEPVDRKVSRILNQAAAREQGI
                     SSASLTDARARLEEFGASTNEVVLNAETVLQLAVKYGHELQSKRDTLYSSALSLRSAR
                     RAAGLQVSGALNYIMGVSGEAEDENSGSADLSVSRLLFTGGDASVTAASSMRESPAGT
                     NGTEATAYSSSIRLKLNQPLLKGAGYTVSHEELVQAERSLVYALRSFAQERQDYAIDL
                     MQQYYSLLSQQTRLRNIGLNVDQSVYLRKRSEALFKVRKAAAIDVLRSQQQELSSKNN
                     LSVAETEYEIAVKRFLITLGLPVDIRVALEGSIPEVRDIRLNEERSVDLALASRLDLL
                     TQRDRFEDAARALWIAKNGLLPALSVYGEAAYRSAETEEFSDQELEEEISAGLSLELP
                     LDRRDERDAMKRAQIALAAAARELRRKEDSVQIEVVENFARLEALRKTVGIEEKNVDI
                     AQKRVQNAVLKFRAGELSNRDVVEAENELLDARNAFIEAKISYEMQRLRLLRNVGLLD
                     VNADGRIMELAADANKEPNTDEAEKE"
     gene            11172..12623
                     /locus_tag="PKK36_02555"
     CDS             11172..12623
                     /locus_tag="PKK36_02555"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR93469.1"
                     /translation="MKLKKNRVFWYAAGLVLLLLVWRGCGRRGKTGTRPVEAVRVEAV
                     RGPLQITITGSGEIMSKNSQKIVPTIRRQATISFLVPEGSRVGKDEVIARLNSEELDN
                     QIEALEESILKQESAVDSAETDLEISNLDAEVSLRSARQAVTDAEQDQEKFLQGDQPM
                     EYRAAELEVQTAESQLGRSERKYEEMKELLDEGFVTEDEVEEARIALEEGRVKLETAR
                     LKLQNVKKYDHPLKTSTKQAVLDKARTELSKIEKTNATRLSNKTREIEGLKRQLERTR
                     EELAKAVKDRDALEIKAPTDGVVHYGDPEVPWRRQDIAVGATVHQGQVLLTIPDLSGL
                     QAAVNIPEADIHAVSTGQTAVITMDALPGNTFEGVVTRVAEVANQNARWMGSSIKEFT
                     VEIEIRGNPSLKPGYSCKAEIITETIADAVKIPVPAVFQEGDEYFVYLAGRMRSQRRI
                     VKPGPASADYVQILEGLEPNETVYLSRPEEEPL"
     gene            12656..13291
                     /locus_tag="PKK36_02560"
     CDS             12656..13291
                     /locus_tag="PKK36_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93470.1"
                     /translation="MEQPLEVLRGVSLEIPDNDFLAIVGPSGSGKSTLLHIMGLLDRP
                     TSGELHFEGKNVAQLSDDELSGLRGSSIGFVFQSFHLISHLSTLENVELPLFYLRVPP
                     GKRREIARECLHRVQMDHREKHLPTQLSGGECQRVAIARSLVTHPPLILADEPTGNLD
                     TKTGSEIFRIFTDLHEEGHTVVIITHDRGLAARVPHRVGIMDGLIVDGGAA"
     gene            13288..14535
                     /locus_tag="PKK36_02565"
     CDS             13288..14535
                     /locus_tag="PKK36_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR93471.1"
                     /translation="MIIWLLELFRDSLRNLVRHKLRSSLTLLGVIFGVASVITMLAIG
                     EGAQRTVLAEIEGLGLKNIIVESVRPPDVTSSTESGSGGSGLLRYGVTHRDVAQIRGA
                     LPHLPLTLVKDVKDRVYCGSRRLNVETRGVTPEYFEYFDSALIQGQPLSFLHEREAMP
                     VAVLTAEAAAQLPGIGGAVGKNLKIGAKHFRVIGVVSITTRGNKALVFIPSRTAESQF
                     GDTSIKQEAGRMEFTRNEVGQVVMLAGSESEVPGVAAAVKRIFTKNHVDADYQITVPL
                     DILKSRQRTQQIFNFVLIAIACISLLVGGIGIMNIMLAIVVERIREIGIRRAIGARRF
                     DIFMQFLSEALTMSVLGGILGCLLGIGMCPVATAWTGWPAIINLRAILLSFAVSFSVG
                     VIFGTAPALHAARLDPVEALRYE"
     gene            14620..16128
                     /locus_tag="PKK36_02570"
     CDS             14620..16128
                     /locus_tag="PKK36_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93472.1"
                     /translation="MNVETFFRHWGLEEDPFQAEEARNDQVYLRTMGEMTHPDFDKIF
                     GSPRDPGTAVVFGDKGSGKTAIRLLIEKRLEEHNRDCPNEKVWVVRCDDLNPLIDRVL
                     HSTGHKEATTAALKEIRLEDHMDYILSLSITRLIDFLLGDKIGIEKARRARRRLRRMS
                     RQNRIDFAELAMIYDQAKGSKPMERWHRIRSILRINPFINQRTSKSTIAFTVLAAAAF
                     ASGAYLYGAKTPLMFAGLGVSLVSTLWLIYAWIRQFFKVAALARKLKHEVRIMDRTTA
                     QLRAKLLSLSPRVLDAQPLPVPGASDSRYELTSRLMRILKEVGYVSMVVLFDRVDEPV
                     SVNGQPDKMKAIVWPMLNNKFLQQSGVGVKLLLPLDLGHRLKREDTEFFQQARLDKQN
                     MIERLTWTGATLYDLANQRIQSCVGEGEYRAKRLNDFFDSGVTTQDLVDALDQMHQPR
                     DAFKFLYSVIQEHCRNVSDSEPVWQIPKLMLDQMRRQQSQRLQEFYRGLSPA"
     gene            complement(16216..17340)
                     /locus_tag="PKK36_02575"
     CDS             complement(16216..17340)
                     /locus_tag="PKK36_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNR93473.1"
                     /translation="MTKKQAPEIVSRKAASKKISDTAQAIYNDIMKGTKKPSMQFPVR
                     SLANVKYDVKQGYFKLLGKRVTRTLSYTTVKTFAQSMRLLATTKNDLLDRDDIAGKRE
                     VYYNSKSWGECRFDEQPESDALLDDMEAMLGINREQLGYIPEERGGDVTGALTVIDKD
                     PATGDPIKINCAKLGTGAWSIPSRVEHLEFQTKAKFILVVETSSLFQRLVHHRYYENA
                     NCILISMSGVPTRACRRFIRRLSDDQKLPVLAFTDGDPYGYCNIYRTLKAGSGQAAHI
                     NEFFCVPNVRYLGVTPQDIIDYNLEDATHPLEEVDIKRAKDALKNDPFIRHHREWQQA
                     LEQMLKMGVRIEQQAFAKHGLNFVLDTYLPQKIKKGKFLP"
     gene            complement(17356..19053)
                     /locus_tag="PKK36_02580"
     CDS             complement(17356..19053)
                     /locus_tag="PKK36_02580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNR93474.1"
                     /translation="MASAEKNTNKKKTAKRETAESMAQKQREISVSEFFLKNRHLLGF
                     DSPRKALLTAVKEAVDNALDACEEAGILPEISVDIAQTGASRFLINVTDNGPGIVKAQ
                     IPKIFGKLLYGSKFHRLRMSRGQQGIGISAAGMYGQLTTGNSTVVLSKTRSQKHAHRM
                     EVQLDTASNKPTILKEEEVEWLPAFYPAPGEGKETAPVVSHHGTSVSIEMEAAYVRGR
                     LSVDEYVKQCAIVNPHLEIHYRISLLKSADEQNGDSESPWTTFRRVVKTVPEPPQEIK
                     PHPHGVELGMLMKMLKDSAARNVCSALAHDFSRISSSTAHEICERAQISPKARPSTIA
                     HRESEALFKAIQETKLMRPPTDCLSPIGEEQLTAGLKKEVEADFYTAITRKPSVYRGN
                     PFQVEVAMAYARPGASEYLGGDDPVRVLRFANRVPLLYMAGACAITRAVSGVNWRAYG
                     LNQPKGGLPVGPLLVSVHIASVWVPFTSESKEAIAHYPEIVREITFGLQECGRQLSVF
                     LSRKKRAAEAERKRNYMEQYIPHLALGLRNVLDLDDKQEKKVIASLHSMLEHTHLQI"
     gene            complement(19203..19760)
                     /locus_tag="PKK36_02585"
     CDS             complement(19203..19760)
                     /locus_tag="PKK36_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR93475.1"
                     /translation="MKKTALFIAAALTAASALAGGGLGIFGTYWDADDTDDPAIGGGI
                     KLKAPLMPNLCMELRGSYLVEFADGTDEESLIPLEAGLVIDFPIAEQLTLYAGGGAGY
                     FISPEYEVDDGMGGEVDVDLDDEVGYYAVAGAELKLNESVALFAEAKYTAVEFDGAEV
                     EGMDVDFEDSEMKGLGVNAGLMLLW"
     gene            complement(19874..20806)
                     /locus_tag="PKK36_02590"
     CDS             complement(19874..20806)
                     /locus_tag="PKK36_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93476.1"
                     /translation="MACALLAGLSAGCATAPVSNDVKAGAFRSKAKTPQNFWLTVYDS
                     ARGPVVSGGHRLHPNQSVAMPFLSPRKSELPLISVQLLREDIYTALVDTSAGESWAEI
                     DDALAMDIVPLGPPDIFREPLHLRNAGRGMLALAETLRFDQLYVENALIFTRLEDAPG
                     DIGRGLKPNLVLGGDFLKQFRYFQINWPGRTVAFSATHPYRPNPDRLLAELPLRRLNG
                     ACVVEAVIDGKTHWVVLDSAGNYELAMTGAEGPAIRQMTLGNLVLRHVRVTPHADLQI
                     GWPGYPKVGTGILSKFKVTVDNKLGTVYIEKPAN"
     gene            complement(20878..24951)
                     /locus_tag="PKK36_02595"
     CDS             complement(20878..24951)
                     /locus_tag="PKK36_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNR93477.1"
                     /translation="MLFSLLVFCLLVPPALLAADDTIFVSSCPERKQFTWEEIQKLAQ
                     ADWLKKTVASDEFQSGKPPPFDDSTKAYIWSLITTPELGPAGGGVTLINLSGAGLSGL
                     DTCLTHLGGILAVVQLYNDYKDNNPAMQVNFAKSASFWSIGTFSVFSTALKTAAVGVG
                     LFDYTLNKLGSATSSMRYEFWEKAYAKYYDKKYGYRVTTKWDAGLGRNVIDQDDTEKW
                     IDLIFRQHGGDVDKALKEGFSDFWKDPEGCALEVGMNPYGKFVQNILKDDQESIRQNY
                     LRHVLPSVRTAFAWKKDRAQRDLVIEFQIAADKIYDELHKELVFFGWVTDKDGWPLEG
                     AEVQLFTEDPVITRASGKYEIRIKACKLYDSFQIRGQRQAELTARMDDPLSDTDYAEL
                     KRVDIAYVAGTPLKEQGPHNIVIATREIREIRISPSDITIKEGLSASVTVLAVLSDGS
                     ESDITDRITWFAAEESIARADAAQITAGKPGTTEITAEYQGKKSGPCRVTVIPAKELL
                     KLETGPSPLNLKPGQSAQLQAWATFSDGQREDVSQDPACRWQAAGTTVSLTRNEVTAI
                     KAAPGQAAPQITATYTFGGKSLSASTQVNIEEEAQILAFKLNKTEVVMGLNENVGLRA
                     SAIIGKGPLTTPADMTDKVSWSASDRQIIVQTAQGVIVSGSAEGACDVTATLKQKDGS
                     VLTAVCRVTVMDKSAPLPEVDFTISPPKFSHQIGEKITFTQNISTKDLKAYDFIWFIG
                     NEEQNGLSASRIFDKSGNYTVRLVLRSRKTGKEDALSKTVRIEALPDADCSISVPPGP
                     YSAPATITFSALCTDPAAAISSLRWYIDGQLVQEGESRTFAHTFQESGKYLVKLGMRT
                     GSNFDEGVRTLVIAVGEPELAAQTSARQVNRFETEVSNEDLSVAGSCWIGGDGKWSDW
                     LVFDRVGPVLKHVFCTGDAEGGYNTGYLVYAPGPGKLLFRVYGYNFQQRRGRMVYDGS
                     LDIHGKQVDAASLRIADCQPRAAVIEWRCTDGSLCKARIFKTPESGYVTGGAQDMGCI
                     EGRPGTQARGTLKQALESAARQKQINPGEVLGPQTEGLKEMSKELDFGTPECMYTIRG
                     NLPNANGGTVEVYVFDDSRAAENGILKWKKYFTSLGDRYETDVTPVSFHNLQAFTWML
                     YYSEKTEKDAKKMAQSSKESRETFKDWIGYRHYGMAWIHDNTWVSLYTKQNTAAGKSD
                     ARNLAETVYSEMAAVAAGRFYCEGDEVAIREFTYLNSIWPARLQKGRLEVRGAGGSSG
                     KWRESEWITVASGVTEFDGDGTSKGPVVIYRTGKKWHIVMIEQDSGKISNHRSFDEPV
                     EIIRTGQYGAVIQSGDQCYDYSWNKLQKINCKTGLYE"
     gene            complement(25001..25741)
                     /locus_tag="PKK36_02600"
     CDS             complement(25001..25741)
                     /locus_tag="PKK36_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93478.1"
                     /translation="MRLVFSVMLGMLLAAASGCATGKAASLQRKNAALESQLAAEKAA
                     AEKLQQQLAALKKINSTLQKEKGVRVEETAAVRSQIRSFVQLQVESLRDFSQNAELFD
                     SVGGELIARDKTGGENLLLVDMQNALLGGATLIGGRLYGSSGTRAVFCLLRPQDNKLA
                     VVWMSKVFNIPAEGVFKVSFDTPVTAKKDDVIGIYCPAKVGIPYDIGTGDTRTMTGPV
                     DTGMVIPMDSLQGGQKRAYSFGVNGFFD"
     gene            25939..26745
                     /locus_tag="PKK36_02605"
     CDS             25939..26745
                     /locus_tag="PKK36_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR93479.1"
                     /translation="MVEVFTGWHPVLQAFAGTCFTWALTAAGAAAVFLHREPSQRSQD
                     LMLGFAAGVMIAASYWSLLAPAISMSEHLGWLKWFPATTGFLLGGVFLRLIDRILPHI
                     HPESQMEDGMHATWKRAVMLVTAITLHNFPEGLAVGVAFGAAAAGDSAYTIGAAIALA
                     VGIGIQNLPEGIAVSVPLRRTGMRARKSFYYGQLSGMVEPVAGVLGALAIMAMKPLLP
                     YTLAFAAGAMIFVVVEDAIPEAQSHGHNDLVTMGAMIGFAIMMTLDVALG"
     gene            complement(26768..>27299)
                     /locus_tag="PKK36_02610"
     CDS             complement(26768..>27299)
                     /locus_tag="PKK36_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR93480.1"
                     /translation="LVGQYIGRREPEHAERAAWNAWQIGMLYMLLTGITFVLFPQFYY
                     SLFVNRETSGIPLSDLLPVGRILLLILPLWGIMDASNLIIGHALKGAGDTDFVMYYSV
                     TMAWLLLVPGQLLIVNVLKGSIIWCWLWTAFYIAVLGLGYILRFRRDRWKSIDLMGRQ
                     PPTEPARGAAEALVVD"
BASE COUNT         5391 a         7923 c         8419 g         5566 t
ORIGIN      
        1 atgcgattat cacgcacccc ggactgaagg ccgcgccgac caaatgggag tcgtcggtat
       61 gacccggcag gagcagaacc catgaaggcc gaacagaccg ccccgcggtt cagggatttg
      121 aagatcaaga tattttctga cggagccgac ctggattcca tcatcgaagc ctaccagaag
      181 ggggatgtca gcgggttcac gacgaatccg acgctgatgt cgcgcgccgg catcaccaac
      241 tatatgacat tcgcaacgga ggtgctgcgc gtcgtgacgg atctgcctgt gtcgtttgag
      301 gtgttttccg acgatttcgc cgagatgaag gaacaggccc tgaagctcgc cgatctggcc
      361 gagaatgtgt atgtcaagat tcccgtcacg aacacgaagg ggaaatcggc ctgtccgctg
      421 atcaaagacc tggccggccg tggcctcaag ctgaacgtga cggcgatcct gaccctgaag
      481 caggtcgagg aggtcgcggc ggcgctgaat ccgaaagtgc ccgccattgt ttcggtgttc
      541 gcggggcgca tcgcggacac gggccgcgat ccggtgccgt acatgaaaga atctctgaaa
      601 atcctgaagc cgcttccgct ggcggagctg ctgtgggcga gcccgcggga gattctcaat
      661 ctcgtgcagg ccgacgagat cggatgtcat atcatcacgg tgacgccggc tgtgctgtcg
      721 aaggccggcg ggctgggccg cgatctcgcg gaactctcgc tcgacacggt caggatgttc
      781 tataacgacg cgcagggcag cggattcacc atttgaaagg cagggagcca tgatcatcac
      841 gcgcacacca ttccggatca cgctgggcgg cggaggcacc gacctgccgt cgttctacga
      901 agaacacggc ggctatgtgt tttcaatgtg catcaacaag tacatgtacg tgatgctcaa
      961 tcgccgttcg gttgccgacc ggaaaatcgt gatccgctac agcgacgtgg agacggtgaa
     1021 cacggtggat gagatccgcc atccgcttgc gcgcgaagcg ctgcgcatgc acggcgttca
     1081 cgagaacatt gaactgacca gcattgccga catgccggcg aagacgggcc tcggctcatc
     1141 gggatcttat ctggtgggcc tgctgacggc catacatgcc tacaagcggg agccggttgc
     1201 gatggagcag atcgcaaagg aggcctgccg gatcgaaatg gacatcctga aggagccggt
     1261 gggaaagcag gaccagtaca tggccgcgct gggcggcttc caggcgctgg aaattgaccg
     1321 cgacggcacg gtgcgcgcgg agagcgtgcc ggttgacttc atgacggtga gcgaactggt
     1381 gctgaaggcc cgcatctatt acacgggcat tcagcgttcc gcgacagccg tgctgaagaa
     1441 tcaggatgag gcggcgcgcc ggaaagagcg cgcggaccac aagcaggtgg tggacagcct
     1501 ctgccgcatc aaggagatcg ggaagggaat taaaaaagcc tttgacgact gcgatctgga
     1561 tgcattcggt cggatgatgg atgagcactg gcagcacaag aagagcatgt cgaaaagcat
     1621 cagcctgtct gtcctcgacg agctttatga cgaagtgaaa ttgcgctttg gcgtgctggg
     1681 cggaaaaatc atcggggccg gcggcggagg atttttcatg ctctactgtc ccgggaaggg
     1741 ccgcgagctg gatgaataca tggcctcaaa ggggatgccc cgcatcaact gcatgcccgc
     1801 ccgtgaaggc gccaaggtgg tcaccgacct gtttgaatag tccgcgcggt cacgcgcggc
     1861 ggggcaggcg gccggcgatc cagtccgctc cttctctgat ggtctggacc gcatagctgg
     1921 atttcaggag gcggcccagt ctccgggact gccagcgagg actgcggacc acgcggcggt
     1981 atattccggt gatctgcttc tggagcgctt cggctccggc ccgtgcgttc cagacggcct
     2041 caggccggtc cagtatccac gggtcgcgcg tccagcggag ccagttgtca atgtccgatg
     2101 ccccgcagtc ggcggctgcc gagcggtaga gggggctgtc catgcagaac ggaaaaggtt
     2161 ccaggacaat atgggcggcc gggagatgat gaatcaaagc cacagctctt cggacggcgc
     2221 ccttcttctc ttcgggtccg ccgatccaga gacgcacaaa cacctcgacc ccggcttttt
     2281 cgagcgagga aatcgtgtgt ctcaatgttc tggccctgaa agggacgccg aatctttcct
     2341 gcagttcctg gtcgcaggag ggcacgatga aatcaactct gcggcagcgt gttgccgcca
     2401 gttcggcaat caggtcgtcc tcgatgcggc ccgggttcag ctgcaggccc cagggctgct
     2461 tgttgtagat gcggttcagc gcccggcacc actcgcgcag gcggccttca ttccagacgc
     2521 ccggagggtc ggtcaggaat atgtcggtga caccgagatc ggaagaggcg cgcacggact
     2581 gcgccagttt gtccatgggc cagaggcgca gcggttcgcg aatcaggccc atggcccggt
     2641 cgcaggcttc ctgtgtatgc ccgcgcgaga ggcgcatgcg ggccggctgc cgcccggcct
     2701 ggtccggatg gtgatacgcg cgccagaaga ccctgcccca gtccggcagg gagaggatgt
     2761 tcagatccgg catccatgag gtggaaatgt ttttgccggg gaaattcagt ccgggaacat
     2821 tctgaaggcg cttttcggaa tcgatatggt ccagcagggc gcgcaggatg ggctcggggt
     2881 cgcccgaaag ggcgtaatcg gcccgcgcaa cggcatggat gtgttccggg aactgggagg
     2941 gaaactggcc gaacatcacg gtgcgtatct ccggccatct gcgcttggac agctccagta
     3001 cggcggcaga ttctccgatg ttggtcaggg atgtgtgcac ggcgatgatc ttcggtccct
     3061 ttacggcgtt cagcgcatgt tcgagttcca gctcaagatc agtgaacaga cggcagtcga
     3121 cacaggtgct cagatgccgg gagtgaccct tcagataggc ctgcagacag aggacatccc
     3181 agtccggcgc aaacgtggcc ggatttttcc gcgccagccg caggggcgcg gctccggcgg
     3241 ttccctccgg gggctgaatt aaaacaacat gcatctgatc tgctccctgg acaaatcgtc
     3301 gcaaaaactg atttcaatat gtatacagcg ggaatagaat gcatgcaagt tgcaactggt
     3361 cccggatatg aagatcatag actgcgatgt gctgatcata ggaagcggcc tggcgggcct
     3421 gacggcggca tggcatctgc gtgatgccgg ccgcatcgcc gtggtctgca agcgggaccc
     3481 gatggagagc aacagcaatc gcgcgcaggg cggcgtggcc tgcgccatag attcggacga
     3541 cagcgtggaa gaacatgtgc gcgacacgct gggcaccgga ggcggtcttt cgcgcgaggc
     3601 ccgcgttcgc gacattcttt cggccggcca ggccggcatc cgcgagctgg agaagatcgg
     3661 cgtgcacttc agccggcgcg agggtgcggc ggattatgat ctcggacagg agggcgggca
     3721 ctcccatcgc cgcgtgcttc atgccggtga catcaccggg catgaaatca tgcgggtgat
     3781 gaccgagcgc gcggcggcgc agtccggcat cacgatctat ccggaaacca tggccattga
     3841 tcttgtttcc accgcttggc tcaggcgtcg cggagacaac cgctgcgtgg gggcttattt
     3901 cctcgactgc aaaacagaga agatcctggc cttccgcgcc ccgcatacgg tgctggctgc
     3961 cggcggcaca ggaaaggtct atctttatac cacaaactcc gatgtggcca ccggcgatgg
     4021 agtggccatg gcctggcgcg cggggatccc gattcaaaac atggagttca tccagttcca
     4081 tccgacctgc ctctaccatc ctgccgccaa atcattcctc atcagcgagg cggttcgcgg
     4141 cgagggcgct gaactgatca acgcgcgggg cgaggcgttc atgaagcgct acgacccgcg
     4201 cggtccgctc gcgccgcgcg acatcgttgc ccgcgccatt gaccatgaga tgaaaatcag
     4261 cggcgacgca tgtgtctatc tggatattac gtgcaagggc gaggaatttc tgcggcgccg
     4321 tttcccgaac atctacgcca cctgtctcaa ctacggtttc gacatggcgc gtgctcctgt
     4381 accggtggtc ccggcggccc actactgctg cggcggggtg gaggccggcg tggacgggcg
     4441 cacaaggatg aaaggcctgt atgtcatcgg cgagaccgcc tgcaccggtc tgcacggggc
     4501 gaaccggctg gccagcaact cgctgctgga ggccatggtc tgcggacgca gggcggcgga
     4561 ggcgatttgc gccgcgccgg ccgaggccgt ggccggaatc aaaatccccg attggcagta
     4621 cggcaacgcc gtgcccagcg acgaggcggt ggtggtcgag cacaactgga atgaggttcg
     4681 tcagtgcatg tgggactatg tgggcattgt ccgcaccacc aagcgcctgg agcgcgccgc
     4741 ccgccgcatt gacaacctgc agcgcgagat ccgggagtat tatctcaatt actttgttac
     4801 acgcgatgtg ctggaactgc gcaatatcgc cgcggttgcc gggctgatca tccgctgcgc
     4861 ccagcgccgc cgcgaaagcc ggggactgca ctacacactg gactatcccg gcctcgatga
     4921 gtcccgcgac ccgcaggaca cgattatcgc cgataaaccc ggtggttcca ttgccacggt
     4981 tgaataatcg gattgacgag tccgcggtgt tcgggtttcc ttaagtcatg tcaaagctta
     5041 tcagagtgga actcggggac cgttcctata acattcatgt ggctccggga ctgctggcgc
     5101 agtgcgggca gttctgccgt gacctgaagc tggggcaccg ctgcctgatg atctccgacg
     5161 ccaatgtggc tccgctgtac ggccacatcg ttcgcgaaag cctgctggaa gccggtttta
     5221 tgccccatgt ctttgtggtc ccggccgggg aaacatctaa aagccaggcc accctttcac
     5281 ttgcctatga tgaagccctg cttcacggca tggaccggaa atccttcgtg gtggcgcttg
     5341 gcggaggggt ggtgggcgat ctggccggat atgccgcagc caccttcttg cggggaattc
     5401 cctttgtcca gattcccacc acgctgatgg ccatggtgga cagctccgtc ggcgggaaga
     5461 ccggcatcaa cctgccgcag gggaagaacc tgatcggggc ttttcatcag ccgtccgcgg
     5521 tgatcgcgga cctttccgct ttcacgaccc ttccggaccg cgaatatatt tccgggctgg
     5581 cggaagtggt gaagtacggc gtgattcagg atgccgattt cttcgcctcc ctggagaaga
     5641 atgcggacag aatacttgca cgtgaaccgg aaacgctggc acaggtggtg gcgcgctgct
     5701 gcgaatgcaa ggcggaggtg gtgcggctgg atgagcgcga atccggcctg cgcgccatcc
     5761 tgaacttcgg gcacacgctg gcgcacgcca ttgagcaggc cgccggatac gggaaattcc
     5821 tgcacggcga ggccgtggcc atcggaatgg catatgccgc cgcgctgtca gtgtctgtcg
     5881 cgggcctctc caatgaagcc gcggagcgca ttattcgcct gcttgagaag tttggcctgc
     5941 cggctgcagt ttcgggcctg aactggagcg cggttcgcag agccatgaag acagacaaga
     6001 aaaacgccgg gggaactgtc cgttttgtgc tggcccgtga aatcggccgg gcggagccgg
     6061 gcattgaagt cagtgaagaa cttctggaag aggtttggaa tgtcatcggt cagtgaatgg
     6121 gtcgttcgcg aatattttga ggctctgggt tttctggtcc agcagccggt aaaataccag
     6181 gtggccgccc gccggaaacg cgccgaagag gagattgatc tgctggtcat gaatccgctc
     6241 ggcggggatc atgccctgcc ttcaaagatc ctgtggaaat cgccggatct gaggaatgtc
     6301 acccgcgccg tggtcagtgt tcggggctgg cacaccgaca agttcagccc caagacgctg
     6361 gagctttcgc cggaggtttt ccgttttgcg gaagatgccg ccatgcgcaa ggcttccggt
     6421 gtgctgggcg aggggcctgt ggccagaatt ctgtgcctgc cggatctcac cagcacggag
     6481 tcgcttcgca aaaggacgct ggaaatgctg cgcggaaacg gcattgacgg cgtgcttctt
     6541 ttccgcacga tgcttctgga actcgtcgcg catgtggaga cgcagaagag ctacgaaaaa
     6601 tccgacctgc tgcagatact gcgtctgatg aagaactacg atctcatcaa ggacccgcag
     6661 atggaccttt tcgcctccaa gcgccgcaag cggaaggcct gacgtgacgg agcgcgaagc
     6721 ctacattgcc ctcaacatgc ttgacggcat gggacccgtc cgcgcgcgca atctcatcga
     6781 acggctgggc tcccccgaag cggtctttga cgccgaaccc gaagcactga tggaggcgaa
     6841 gggcatcggg caggaaatgg ccgagtctat cgtgaaccag cgcgacgaaa tcaaccctgc
     6901 ggaagaaatc gaaaaaaccg aagcgctggg cgggcgcatc ctgacgtgcc ttgacgatga
     6961 atatcccgcc gcgctccgca ccatctacga tccgccgctt gcgctttaca tccttgggaa
     7021 actggttccg gaggacaagc atgcgatcgc gctggtgggt tcccgccgca gttcgcatta
     7081 cggccagcag acggcggatc ggctggcgtt ccagcttgca aaggtcggct acaccgtcat
     7141 cagcggcctt gcgcgcggca tagacaccga ggcgcacaag ggatcgctca aggccggcgg
     7201 ccggaccatc gccgtgctgg gcggagcgct ggacaaaatc tatccccccg aaaacgcggg
     7261 ccttgcggag cagatcgcgc agcacggcgc cgtgatctcg gaattcacgc tgggacgcga
     7321 gccggacaaa accacgttcc cgtatcgcaa ccgcgtggtc agcggaatgt ccatgggcgt
     7381 cgttgtggtg gaagccccgt tcaagagcgg atcgctgatc acggccgagc aggcgatgga
     7441 ccaggggcgg agcgtgtttg cggtgcccgg ccgcatagac aatcccaacg cgcgcggatg
     7501 ccatatgctc atcaagagcg gcgcaaggct tgtcgagaat gtggacgaca tcctgcagga
     7561 atttgaaatg ctcatcccgc aggagcagaa ggatgtggcg aaagcggcct cgcgccggcc
     7621 ggaggtcatg ctcaccgacg acgaggaaaa aatcgtccgc gcgctcggaa gcgacacgct
     7681 ggacatagac acgctggcgc ggaatgcgga actcaacatc gcatcggtca gcggcctgct
     7741 catcggcctc gaactcaaaa aagtcattcg catgcttccg ggccgcctcg tggaactcat
     7801 cgcccgcgcc gaagatttct gaaaagttcc agccttcctg agttctgatt cgctttgtga
     7861 attttgtgct tcttgtggca aatgtccgga aatatgaaaa aatggccaca aaaagcacaa
     7921 aaatcgcaaa aaagtgccaa aaatggccaa aaaacgctca aaaagccgaa aaaacggcta
     7981 aaaattcccc aaaaatgccc ataaatagca aaaaacggac gaaaatgcgc agatcattca
     8041 tataggacta aattagtcct atatcactta atgcgatgag aattccggtt aacccgttca
     8101 ttgagaaatt caaccgaggc ccttcaggcg ggcggtggag agcatgccgc aggcggcggc
     8161 gatgtcctgg cccttggact tccggatcgt ggtgacgaat ccggcgtcct tcaatatatt
     8221 ctggaagatt tcgacctttt cgcgggaaga aggaacaaag gaacttcccg gaacggcatt
     8281 gaacgggatc aggttgatcc ggcagtccag atacttgaga agccgcgcaa cggccttggc
     8341 gtgcgcgggc gagtcgttca cgctgtcaaa cagcgcatat tcaaacgtca gccgccgctg
     8401 cccgcggatg gcggcgcggc ggagcacttc aacgacttct ttaatcggat gctgtttttc
     8461 caccggcatc atggccgcgc gctcttcgcc aaaaggtgag tgcatgctga tcgccacgtg
     8521 gcatgagcag gatttgatca gtgtctccag cgcgggcaga atgccgacgg tggaaacggt
     8581 caggcgggtg gggctcttgg catagccgta atcggccgtg aagatttcca cggaacgcag
     8641 aacggcctcc agattgtcga gcggctcgcc catgcccata tacacgacgt tggtgatctg
     8701 gtcgcgttcc gggcagctgg cgtactggtt gaggatttcc gccgccgaca gattgccctg
     8761 aaggccctgc tgtccggtcg cgcaaaatgc gcaggcgcgg gcgcatccga cctgcgtgga
     8821 aatgcagagc gtggcgcgct cttcgtcggg gatcagcgcg gtctcgatga attttccttc
     8881 gcctgcgggg aacagatatt ttttcgttcc gtcggccgat tccgcgcact ccacgggcgc
     8941 ggagcggccg gtggtgcagt ccgcggccat ctgttcgcgc agggattttg aaagcgacga
     9001 catggcgtca atggtttccg cgtgatgctt gtagatccat tcgcagatct gccttccgcg
     9061 gaaggcgggc tgcccgagcg tcgcggcgag ggcgacgagt tcggcctgag tctgtccaag
     9121 aagagtaatc attggcgaat cagtttctcc tgaagcggca gatagcgcag ataaagaaac
     9181 aggtcaagac agaccatccc gcagttgaga gcgtagagga aaatgacggg atcaaaatga
     9241 tagaacgcct tgtgaagcac gccgcacagg tagccgatga tcacgatgac catgaaaaca
     9301 gggcttttcc cggcgaccac ttttgtgcgc agggctttgg cgatggacac gggccagctt
     9361 gccccgaaca gaagcagcat ggcgatttca aacgggctca taacattcct ttcattggta
     9421 gggcggccac tccgtggccg ccgcggcgcg cacggagtgc gcgccctacc ggcgatctca
     9481 aacagtagta attggagtgc gaagtaatat aacgaggatg caaatgcaaa catttaaagg
     9541 ggaacaggat tgctcccgaa gttgcggctg ttgtagagtc tgttccatgc gatgcccggg
     9601 ctacatattc gtctcgctca tcaccaccgg catactgacc ggctgcggga cctttgagcc
     9661 ggtggaccgc aaggtgtccc gcattttgaa ccaggccgcc gccagagaac agggcatatc
     9721 ttcagcatct ctgactgacg cccgtgcccg ccttgaggag ttcggagctt cgacaaacga
     9781 ggtggtgctg aacgccgaaa cggtgcttca gcttgctgtg aagtacgggc atgaactgca
     9841 gtccaagcgc gacacgctgt actcatccgc gctctcgctg cggtcggccc gccgcgcggc
     9901 cggcctgcag gtgtcgggcg cgctgaacta catcatggga gtgtcgggcg aagcggagga
     9961 tgaaaattcc ggctcggcgg atttaagcgt gtcgcgcctg cttttcacgg ggggggatgc
    10021 atcagtgacc gcggcctcct ccatgcggga atctcccgcc ggaaccaacg ggacggaggc
    10081 gacggcatac agcagcagca tacgcctgaa actgaaccag ccgctgctga agggcgcggg
    10141 gtatacggtc agtcatgagg aactcgtgca ggccgagcgc agtctggtct atgcgctgcg
    10201 cagtttcgcg caggagcgcc aggactatgc cattgacctg atgcagcagt actattcgct
    10261 tctcagccag cagacgcggc tcaggaacat cggactgaac gtggaccagt ccgtttatct
    10321 ccgcaagcgt tcagaggcgc ttttcaaggt gcgcaaggcg gccgcgattg acgtgcttcg
    10381 ctcgcagcag caggagctgt cctctaagaa caacctgagt gtggcggaaa cggagtatga
    10441 aatagccgtc aagcggtttc tgatcacgct gggcctgccg gtggacatcc gggtcgcgct
    10501 ggagggcagc attcctgaag tccgggatat caggctgaat gaggagcgga gcgtggacct
    10561 ggcgcttgcg agccgcctgg atctgctgac gcagcgggac cgttttgagg atgcggcccg
    10621 cgcgctgtgg attgcgaaga acggcctgct gcccgcgctg agcgtgtatg gcgaggcggc
    10681 ctatcgcagc gccgaaaccg aggagttttc cgatcaggaa ctcgaagagg agatatccgc
    10741 cggactgtcg ctggagcttc cgctggaccg ccgtgacgaa cgggacgcga tgaaacgggc
    10801 gcagattgcg ctggcggccg ccgcgcgcga gctgcggcgc aaggaggatt ccgtccagat
    10861 tgaagtggtg gagaatttcg cgcggctgga agcgctccgg aaaaccgtgg gcatcgagga
    10921 gaaaaacgtg gacatcgccc agaagcgcgt gcagaacgcg gtgctcaagt ttcgcgcggg
    10981 cgaactttcc aaccgcgatg tggtcgaggc ggagaatgag ctgctggatg cgaggaacgc
    11041 cttcattgag gccaaaatca gttatgaaat gcagcgcctg cgcctgcttc ggaatgtggg
    11101 tctgctggat gtgaatgccg acggccggat catggaactg gctgctgacg cgaacaagga
    11161 gccgaatacc gatgaagctg aaaaagaata gagtgttctg gtatgctgcg gggctggtgc
    11221 tgctcctcct tgtttggcgc ggctgcggcc gtcgcgggaa gacgggcacg cgcccggtgg
    11281 aggccgtgcg cgtggaggct gttcgggggc ccctgcagat caccatcacg ggctccggcg
    11341 aaatcatgtc caaaaactcc cagaagatcg ttccaaccat tcgccggcag gccaccatca
    11401 gctttctggt gcccgagggc agccgcgtcg ggaaggacga agtgattgcc cgcctgaatt
    11461 cagaagagct ggacaaccag attgaagcgc ttgaagaaag catcctgaaa caggaaagcg
    11521 ccgtggacag cgcggagact gatctggaga tttccaatct ggatgccgag gtcagtttgc
    11581 gttcggcccg gcaggccgtg accgatgccg aacaggatca ggaaaaattc ctgcaggggg
    11641 atcagccgat ggaataccgt gcggcggagc tggaggtgca gaccgcggag agtcagcttg
    11701 gccgcagtga gcggaaatac gaggagatga aagaactgct ggatgaaggc ttcgtgaccg
    11761 aggacgaagt ggaggaggcg agaattgcgc tggaggaggg ccgcgtgaag ctggaaacgg
    11821 cccggctgaa gcttcagaat gtgaaaaaat atgatcaccc gctcaagaca tccaccaagc
    11881 aggcggttct ggacaaggcg cgcaccgaac tttccaagat cgaaaagacg aacgccaccc
    11941 ggctgagcaa taaaacccgc gaaatcgaag ggctgaaacg ccagcttgag cgaacccgtg
    12001 aagagctggc caaggccgtc aaggatcgcg atgcgcttga aatcaaggct cccaccgacg
    12061 gagtggtgca ttacggcgat ccggaggtgc cctggcggcg tcaggacatc gccgtgggcg
    12121 cgacggtgca tcagggacag gtgctgctga cgatcccgga cctcagcggc ctgcaggcgg
    12181 ctgtgaacat ccccgaggcc gacattcacg cggtatccac cggccagacg gcggtgatca
    12241 ccatggatgc gctgcccgga aatacctttg agggagttgt gactcgcgta gcggaagtgg
    12301 ccaatcagaa tgcgcgctgg atggggagca gcatcaagga gttcacggtt gaaatagaaa
    12361 tcagggggaa ccccagcctg aagccgggat attcctgcaa ggccgaaatc atcaccgaaa
    12421 cgattgccga cgcggtcaag attccggtgc cggcggtgtt tcaggagggc gatgaatatt
    12481 ttgtttacct tgcgggccgg atgcggagtc agcgccggat tgtgaagccg ggcccggcct
    12541 ccgccgacta tgttcagata ctcgagggac tggagccgaa cgaaaccgtc tatctttcca
    12601 ggccggagga agaaccgctt tgatatcgct ctccgccatc cgcaagacat acggaatgga
    12661 gcagcccctt gaggtcctgc ggggagtcag tctggaaatc ccggacaatg attttcttgc
    12721 cattgtcggg ccgtccggct ccggcaagtc caccctgctg catatcatgg gcctgctgga
    12781 ccggccgact tcgggcgagc ttcattttga gggaaaaaac gtggcgcagc tgtccgacga
    12841 tgagctttcc ggcctgcgcg gaagttccat tggctttgtg ttccagtcgt ttcatctgat
    12901 ctcacatctg tccacgctgg agaatgtcga gttgccgctt ttctaccttc gggttccgcc
    12961 ggggaaaaga agggaaatcg cgcgcgaatg cctgcatcgc gtgcagatgg accaccgcga
    13021 aaagcatctg cccacgcagc tttcgggcgg cgagtgccag cgggtggcca tcgcgcgatc
    13081 gctggttaca catcctccgc tgattctggc ggatgagccc acgggcaatc tggacacgaa
    13141 gaccggatcg gaaattttca gaatattcac ggatcttcac gaggagggcc acacggttgt
    13201 gattatcacg cacgaccggg gccttgccgc ccgtgtccct catcgcgtcg gcatcatgga
    13261 cgggctgatt gtggacggag gcgccgcatg atcatctggc tcctcgagct gttccgcgac
    13321 agcctgcgta atctggtgcg ccacaagctg cgctcgtcgc tgacgcttct gggcgtgatc
    13381 ttcggcgtcg catcggtgat caccatgctg gccatcggcg agggggccca gcgcacggtg
    13441 ctggccgaaa ttgaggggct tggcctgaaa aacatcatcg tggagtccgt ccgcccgccg
    13501 gatgtgacct cctcgacgga aagcggttcg ggcggctccg ggctcctccg ctacggcgtc
    13561 acgcatcgcg acgtggccca gattcgcgga gcgctgccgc atcttccgct gacgctggtc
    13621 aaggatgtca aggaccgggt ctactgcgga agccggcgct tgaatgtgga aacgcgcgga
    13681 gtgacgccgg agtatttcga atatttcgat tcagcgctca tccaggggca gcccctctcg
    13741 tttttgcatg aacgcgaggc catgcccgtg gcggtcctga cggcggaggc ggccgcgcag
    13801 ctgcccggca ttggcggggc ggtgggaaag aacctgaaga tcggggccaa gcatttcagg
    13861 gtcatcggag tggtgagcat taccacgcgg gggaacaagg cgcttgtctt cattccctcc
    13921 cgtaccgcgg aaagccagtt cggcgacacc tccatcaagc aggaggcggg ccgcatggaa
    13981 ttcacgcgca acgaagtcgg gcaggtggtc atgctggccg gatccgagtc ggaagtgccg
    14041 ggcgtggccg ccgccgtgaa gcggattttc acgaaaaacc acgtggatgc cgattaccag
    14101 atcaccgtgc ccctcgacat cctgaagagc cggcagcgta cgcagcagat cttcaatttc
    14161 gtgctgattg cgattgcctg catttcgctt ctcgtgggcg gcatcggaat catgaacatc
    14221 atgctggcga ttgttgtgga gcgcatccgc gagatcggca tccgccgcgc gatcggcgcg
    14281 cgccggttcg acattttcat gcagttcctc agcgaagcgc tgaccatgtc tgtgctgggc
    14341 ggaatacttg gatgcctgct gggcatcggg atgtgtcctg tggccacagc atggaccggc
    14401 tggccggcca tcatcaatct gcgtgcgatc ctgctgtcct tcgcggtgtc cttcagcgtg
    14461 ggggtcattt tcggaacggc tccggcgctg cacgccgccc ggctggatcc ggttgaggcc
    14521 ctgcgctacg aatgaccgcc gccgctgcga aaatactttg tttcctgcgg caaatcggtt
    14581 aacgtgacga cagctggatt ctaaccgtgg gaaggcgcta tgaacgtaga aacatttttc
    14641 aggcactggg ggctggaaga agatcctttt caggcggagg aggcccgcaa cgaccaggtg
    14701 tatctccgca ccatgggcga aatgacccat cccgatttcg ataaaatctt cggctcgccg
    14761 cgcgatcccg gcacggccgt ggtctttggc gacaagggct ccgggaagac cgccatccgc
    14821 ctgctgatcg aaaagcgcct tgaagaacac aaccgcgact gcccgaacga gaaggtctgg
    14881 gtcgtccgct gcgatgacct caatccgctg attgaccgcg tgctgcactc caccggccac
    14941 aaggaggcca cgactgccgc gctgaaggaa atccggcttg aggatcacat ggactatatc
    15001 ctctcgcttt ccatcacgcg gctgattgat tttctgctgg gcgataaaat cggcatcgag
    15061 aaggcccgcc gcgcccggcg ccgcctgcgg cgcatgagcc gtcagaaccg catagacttt
    15121 gccgagctgg caatgattta tgaccaggcc aaggggagca agccgatgga gcgctggcac
    15181 cggatcagaa gcatcctgcg catcaatccg ttcatcaatc agcgcaccag caagtccacg
    15241 atcgccttca ccgtgctggc ggccgcggct tttgcctcgg gcgcgtatct ttatggcgca
    15301 aagacgccgc tgatgttcgc cgggctgggc gtgtcgctgg tctccacgct gtggctgatc
    15361 tatgcctgga tccggcagtt tttcaaagtg gcggcgctgg cccgcaaact gaagcacgaa
    15421 gtgcgcatca tggaccgcac caccgcgcag ctccgggcca aactgctgag cctgtcgccg
    15481 cgcgtgctgg atgcccagcc gctgcccgtg ccgggcgcgt ccgacagccg ctacgagctc
    15541 acctcgcggc tgatgcgcat cctgaaggag gtcggctatg tcagcatggt cgtgcttttc
    15601 gaccgcgtgg acgagccggt ctccgtcaac ggccagccgg acaagatgaa ggccattgtg
    15661 tggccgatgc tcaacaacaa attcctgcag cagagcggtg tcggcgtgaa gctgctgctc
    15721 ccgctggatc tcggccaccg tctgaaaagg gaggacaccg agttcttcca gcaggcgcgc
    15781 ctcgacaagc agaacatgat tgagcggctg acatggaccg gcgccacgct ctacgacctg
    15841 gccaaccagc gcatccagag ctgtgtcggg gagggggaat accgcgccaa gcggctcaac
    15901 gatttcttcg actccggggt caccacgcag gacctggtgg acgcgctgga ccagatgcat
    15961 cagccgcgag acgcgttcaa gttcctctac agcgtgattc aggagcactg caggaatgtt
    16021 tcggattcag agcccgtctg gcagatcccg aaactgatgc tggaccagat gcgccgccag
    16081 cagagccagc gcctgcagga gttctaccgc ggcctgtcgc cggcctgaaa agttccaatg
    16141 attggaactt ttcgcggaaa aacttccagg cattggaact ttctattcag aaacttccag
    16201 tgattggaac ttttgtcagg gcaggaattt cccttttttg atcttttggg gcaggtaggt
    16261 gtcgagcacg aagttgaggc cgtgtttggc gaaggcctgc tgctcaatgc ggacgcccat
    16321 tttcagcatc tgctccagcg cctgctgcca ttccctgtgg tgccggatga aggggtcgtt
    16381 cttcagcgcg tccttcgcgc gcttgatgtc cacttcctcc agcggatggg tggcatcctc
    16441 cagattgtag tcaatgatgt cctgcggcgt gacaccaagg tagcgcacat tcggcacgca
    16501 gaagaattca ttgatgtgcg cggcctgtcc ggacccggcc ttgagcgtgc gatagatgtt
    16561 gcagtagccg tagggatctc cgtcggtgaa ggccagcacg ggcagcttct ggtcgtcgct
    16621 cagccgccgg atgaaccgcc ggcacgcgcg cgtgggcact ccgctcatcg agatcaggat
    16681 gcagttcgcg ttttcgtagt agcggtggtg cacaagacgc tgaaagagcg atgaggtttc
    16741 caccaccaga atgaatttgg ccttcgtctg gaattccagg tgctccacgc gcgacgggat
    16801 gctccacgcg ccggtgccca gcttggcgca gttgatcttg atggggtctc cggtggcggg
    16861 atccttgtca atgacggtca gggcgccggt cacgtcgccg ccgcgctcct cggggatgta
    16921 tccgagctgt tcgcggttga tgcccagcat cgcctccatg tcgtccagca gcgcgtcgct
    16981 ctcgggctgc tcatcaaagc ggcactcgcc ccagctcttg ctgttgtagt agacctcgcg
    17041 cttgcccgcg atgtcgtcgc ggtccagcag gtcgttcttg gtggtcgcca gcagccgcat
    17101 ggactgggcg aaggtcttca ccgtggtgta ggaaagcgtg cgggtgaccc gcttgccgag
    17161 aagcttgaaa tagccctgct tcacgtcgta tttgacgttg gccagcgagc gcaccgggaa
    17221 ctgcatcgag ggcttttttg tgcccttcat gatgtcgttg tagatcgcct gggcggtgtc
    17281 ggaaattttc tttgaggccg ctttccggga tacgatttca ggagcctgtt tttttgtcat
    17341 gttcgtgttc cgtggttaaa tctgaagatg cgtgtgttcc agcatgctgt gaaggctggc
    17401 gatgactttc ttttcctgct tgtcgtcgag gtccagcacg ttcctgagtc ccagcgccag
    17461 atgcggaatg tactgttcca tgtagttccg tttccgttcc gcttccgccg cccgcttctt
    17521 gcggctcagg aaaaccgaaa gctggcggcc gcattcctgc agaccgaagg tgatttccct
    17581 gacgatctcc ggatagtgcg cgatggcctc cttgctttcg ctggtgaacg ggacccagac
    17641 gctggcaatg tgaacactga cgagcagcgg gccgaccggc agcccgccct tcggctggtt
    17701 caggccgtag gcccgccagt tgacgcctga aaccgcgcgg gtgatggcgc aggcccccgc
    17761 catgtaaagc aggggcaccc ggttggcaaa gcgcaggacg cggacgggat catcgcctcc
    17821 gagatattcg ctggctcccg ggcgggcata cgccatggcc acctccacct gaaaggggtt
    17881 cccgcggtaa accgacggct tgcgggtgat cgcggtgtag aagtccgcct ccacttcctt
    17941 cttcagtccg gccgtgagct gttcttcgcc gatgggcgaa agacagtccg tcggcggccg
    18001 catgagtttc gtttcctgga tggccttgaa gagcgcttcg ctctcgcgat gggcgatggt
    18061 cgaaggccgt gccttcggac tgatctgcgc ccgctcgcag atttcgtgag cggtgctgct
    18121 gctgatgcgg gagaaatcat gcgcgagcgc tgagcacacg ttgcgggcgg ccgaatcctt
    18181 cagcatcttc atcagcatgc ccagctccac gccgtgcgga tggggcttga tctcctgcgg
    18241 cggctccgga accgttttaa ccacgcgccg gaaggtggtc caggggcttt cgctgtctcc
    18301 gttctgctcg tccgctgact tcagcagact gatgcggtaa tgaatctcca gatgcggatt
    18361 cacaatggcg cactgcttga catactcgtc aacggaaaga cgaccgcgga cataggctgc
    18421 ttccatttcg atggagaccg atgttccgtg gtgcgatacg acgggcgcgg tttctttgcc
    18481 ttccccgggc gccggataaa atgccggaag ccattccacc tcttcctcct tcaggatcgt
    18541 cggcttgttg gaggcggtgt cgagctgcac ttccatccgg tgtgcgtgtt tctgactgcg
    18601 ggtctttgag agcacaaccg tggaattccc ggtggtgagc tggccgtaca tgcccgccgc
    18661 gctgatgccg atgccctgct ggccgcgcga catgcgcagg cggtgaaact tggagccgta
    18721 cagaagcttg ccgaagattt tcggaatctg cgccttcaca attcctgggc cgttgtcggt
    18781 cacattgatc aggaaccggc tggctccggt ctgggcaatg tccacactga tttccgggag
    18841 gatgccggct tcctcgcagg cgtcaagcgc gttgtccacg gcctctttca cggcggtgag
    18901 cagcgccttg cgcgggctgt cgaaaccgag caggtgccgg ttcttcagaa agaattccga
    18961 aacggaaatc tcgcgctgtt tctgcgccat gctctccgca gtttcgcgtt ttgcggtctt
    19021 tttcttgttc gtgttttttt cggctgaagc cataacttat catcctccgg actaaaaacc
    19081 ccgacagatt atcaccggca agaggagttg caaggaagaa cgggagcctg caaaagaacc
    19141 cctcccgcgc ggacattgtc cgcacgggag ggccatccac tgtcaaatgc acctgccgga
    19201 gtttaccaga gaagcataag gccggcgttc acgcccagcc ccttcatttc ggaatcctcg
    19261 aagtccacat ccatgccttc cacttccgcg ccgtcgaatt caacggccgt gtatttggct
    19321 tcggcaaaga gggcgacgga ttcattcagc ttcagctcgg cacctgcgac ggcgtagtag
    19381 ccgacttcat cgtccaggtc aacatccacc tctccgccca tgccgtcgtc cacttcatat
    19441 tccggagaga tgaaatatcc ggctccgccg cctgcataga gggtcagctg ttcggcaatg
    19501 gggaaatcga tcaccagtcc ggcttcaagg ggaataagcg attcttcatc cgtgccgtca
    19561 gcaaactcca caagatagga tccgcggagt tccatgcaca gattcggcat caacggcgcc
    19621 ttgagcttga tgcccccgcc gatggcggga tcgtccgtgt cgtcagcatc ccagtaggtc
    19681 ccgaaaattc cgaggcctcc gccggccagc gcggatgcgg cggtcagggc tgctgcaata
    19741 aacaaggcag tttttttcat cttcgatcct cctgttttgg ggtcactgga accggaacat
    19801 ccggaacagc ggtccacggc aaaaagcata atgaaagaga ggcttctgtc aatgctgcag
    19861 gctgtccggc cggtcagttg gccggctttt cgatataaac cgtgcccagc ttgttgtcca
    19921 cggtgacctt gaatttggag agaatgcccg ttccgacttt gggatatccg ggccatccga
    19981 tttgcagatc cgcgtggggc gttacgcgca catggcgcag aacaagattt cccagcgtca
    20041 tttgccggat ggcggggcct tcggcgcccg tcatggccag ctcgtaattc ccggcgctgt
    20101 ccagcacgac ccagtgggtt ttcccgtcaa tgaccgcctc caccacgcag gcgccgttga
    20161 gccgccgcag gggcagttcc gccagaaggc ggtcgggatt cggacgatag gggtgtgtgg
    20221 ccgaaaacgc gactgtgcgg ccgggccagt tgatctggaa atagcggaac tgcttcagga
    20281 agtctcctcc gagcacaaga tttggtttaa gcccgcggcc gatgtccccc ggtgcgtctt
    20341 cgagtcgggt gaagatcagt gcgttttcga catagagctg gtcaaaccgc agggtttccg
    20401 ccagcgccag catgccgcgg ccggcgttgc gcaaatgcag cggctcgcga aagatgtccg
    20461 gcggacccag ggggacaata tccatggcca gcgcatcatc aatctccgcc cagctctctc
    20521 ccgcgctggt gtccacgagc gcggtataaa tgtcttcccg cagcagctgg acgctgatca
    20581 gcggcagttc cgatttgcgt ggagacagga agggcatggc gacggactga ttcgggtgca
    20641 gccggtgccc gccgctgacg acggggccgc gcgcggagtc gtagaccgtg agccagaagt
    20701 tctggggcgt tttggctttt gaacgaaatg cgcccgcttt cacatcattt gaaaccgggg
    20761 ccgtggcaca gcccgcggaa aggccggcca gcagggcaca ggccataaaa attctgaatg
    20821 catgcatcat ggacatacgt tgccatgtct gcggcgcggt gacaagcctg caccgcgcta
    20881 ttcataaagt ccggttttgc agtttatctt ttgcagtttg ttccagctgt agtcataaca
    20941 ctggtcgccg gactgaatga ctgccccata ctgcccggtc cgtatgattt caaccggctc
    21001 atcaaacgac cggtggttgc tgattttccc gctgtcctgc tcgatcatca cgatgtgcca
    21061 tttcttccct gttctgtaga tgacaaccgg ccccttcgat gtgccgtctc cgtcgaactc
    21121 tgtgacgccg ctggcaacgg tgatccattc tgactctctc cattttccgg atgagccgcc
    21181 ggctccgcgg acttcgagtc ggcccttctg aagccgtgcc ggccagatgc tgttcaaata
    21241 ggtgaactcc ctgatggcga cctcatcgcc ctcgcagtaa aagcgtccgg cggccaccgc
    21301 agccatttcg gaatacaccg tttcggccag gttccgcgca tcggattttc cggccgcggt
    21361 gttctgcttt gtgtacaggc tgacccatgt attgtcatga atccaagcca tgccatagtg
    21421 ccgatatccg atccagtcct tgaaggtctc gcggctctct ttgctggact gggccatttt
    21481 ctttgcgtcc ttttccgttt tttcagaata atagagcatc catgtgaacg cctgcaggtt
    21541 gtggaacgag acgggggtca catcggtctc atatctgtct cccagactgg tgaaatattt
    21601 cttccatttc agaatgccgt tttccgctgc gcgggagtca tcaaacacat acacttccac
    21661 ggtgccgccg ttggcgttgg gcagattgcc cctgatggtg tacatgcatt caggcgttcc
    21721 gaaatccagt tcctttgaca tttccttaag accctctgtc tgcggcccga gcacttcgcc
    21781 gggattgatc tgcttctggc gtgcggcgga ctccagggcc tgcttcagtg tcccccgggc
    21841 ttgtgttccg ggccgtcctt caatgcagcc catgtcctgg gccccgcccg tgacatatcc
    21901 gctctccgga gttttaaata tccgggcttt gcacaggctc ccatccgtgc agcgccactc
    21961 aatgaccgcg gccctgggct ggcagtcggc gatgcgcaga gatgccgcat caacctgctt
    22021 gccgtggata tcgagactcc cgtcgtacac catccgcccc ctgcgctgct ggaagttgta
    22081 tccgtaaacc cgaaacagaa gcttgccggg gccgggcgca tatacaaggt atccggtgtt
    22141 gtatcccccc tctgcatctc cggtgcagaa gacgtgtttc agcacagggc ccacccggtc
    22201 aaaaacaagc cagtcggacc atttcccgtc cccgccgatc cagcagctcc cggccacact
    22261 caggtcttca ttggatacct ctgtctcgaa ccggttgact tgccgcgccg aggtctgcgc
    22321 cgccagttcc ggttctccca ctgcaatgac cagcgtccgg accccttcgt caaaattgct
    22381 gcccgtgcgc atgcccagtt tcaccagata ttttccgctc tcctggaagg tgtgcgcaaa
    22441 agttctgctt tcgccctcct gcacaagctg gccgtcaata taccagcgca aagaagaaat
    22501 ggccgcggcg ggatcggtgc aaagcgccga gaaggtgatt gtggctggag cgctgtacgg
    22561 ccccggcggt acggatatgg aacagtctgc atcgggaagc gcttcgatgc ggacagtttt
    22621 ggacaaggcg tcctccttgc cggtttttcg cgagcgcagc accagccgaa ccgtgtaatt
    22681 ccccgacttg tcgaatattc tgcttgcgga cagtccattc tgttcctcgt tcccgatgaa
    22741 ccagatgaag tcgtaagcct tcaggtcttt cgtgctgata ttctgtgtga aggtgatttt
    22801 ctctccgatc tgatgagaga atttgggcgg gctgatcgtg aagtccacct ccggaagcgg
    22861 cgctgatttg tccatgacgg tgaccctgca gactgctgtc agaacgcttc catctttctg
    22921 tttgagcgtg gccgtgacat cacaggcgcc ttcggccgat ccgctgacga tgaccccctg
    22981 cgcggtctgc acaatgatct gcctgtccga tgcgctccat gacactttgt cggtcatatc
    23041 cgccggcgtg gtcaggggcc ccttgccgat aatggcggat gcccggagtc cgacattttc
    23101 attcaggccc attacgacct ctgttttgtt gagcttgaag gcaagaattt gcgcctcttc
    23161 ctcaatattc acctgagtgg aggcggaaag agattttccg ccgaacgtgt atgtggctgt
    23221 aatctgcgga gcggcttgtc cgggtgcggc tttgatggct gttacctcgt tccgggtgag
    23281 agacacggtt gtgcctgcgg cctgccatcg gcaggccggg tcctgactga catcttctcg
    23341 ctgcccgtcg gaaaaggtgg cccatgcctg aagctgggcc gactgcccgg gcttgagatt
    23401 caggggggat ggccctgttt ccagtttaag cagttctttt gccggaatca cagtaacccg
    23461 gcaggggccg ctctttttac cctgatattc ggcggtgatt tcagtggttc cgggtttccc
    23521 ggccgtaatc tgcgccgcgt ccgcccgggc tatgctctct tccgccgcga accaggtgat
    23581 tcggtccgtg atgtcggact cactcccatc gctcaggacg gccagcaccg tcacggatgc
    23641 tgacagtccc tccttgattg taatgtcaga cggggaaatt cgaatttccc tgatttctct
    23701 ggtggctatc acgatgttgt gcggcccctg ctctttcagg ggggttccgg ccacataggc
    23761 aatatccacg cgcttcaact ccgcataatc agtatcgctg aggggatcat ccatccgcgc
    23821 cgtcagctcg gcctggcgct gtcctctgat ctgaaaagag tcatagagct tacaggcttt
    23881 aatcctgatt tcatatttgc cgctggcccg tgtgatgacg gggtcctcgg tgaaaagctg
    23941 aacctcggct ccctccagcg gccacccgtc cttgtctgta acccatccga aaaaaacaag
    24001 ttccttgtga agctcgtcat atattttgtc tgccgcgatc tggaattcga tcaccagatc
    24061 gcgctgggcc cggtcctttt tccatgcaaa ggccgttcgc acggagggca gaacgtgccg
    24121 cagataattc tgccggatgg actcctgatc atctttcaga atattctgca caaacttgcc
    24181 gtacggattc attcccactt cgagcgcgca gccttccggg tccttccaga agtcggaaaa
    24241 gccctctttc agggccttgt ccacatcgcc gccgtgctgc cggaatatga gatcaatcca
    24301 cttctcggtg tcgtcctggt caatgacatt ccggcccagg cccgcatccc attttgtggt
    24361 cacccgatag ccgtatttct tgtcgtaata tttggcatag gctttttccc agaactcata
    24421 gcgcatggag gatgtggcgc ttcccagctt gttcagggtg tagtcaaaaa ggccgacccc
    24481 gacggcggcg gttttaaggg cggtgctgaa gacgctgaat gtcccgatgg accagaacga
    24541 tgcgctcttt gcgaaattga cctgcatggc cggattgttg tccttgtagt cattgtagag
    24601 ctgcacgacg gccaggatgc cgcccaaatg cgtcaggcag gtgtccagac cgcttaaccc
    24661 ggcccccgaa aggttgatta aggtaacccc tcctccggct gggcccagct ccggagtggt
    24721 gatcaggctc cagatatagg cctttgtgct gtcgtcaaac ggaggaggct ttccgctttg
    24781 aaattcatcc gaggccacgg tctttttcag ccagtcggcc tgagcgagtt tctggatttc
    24841 ttcccatgtg aactgctttc gctccggaca ggatgaaaca aagatggtgt cgtccgccgc
    24901 aaggagtgcc ggcgggacga gcaggcagaa cacgagcaga gaaaatagca tattgcggcc
    24961 tgcattcata atattccgcg ccggttctgt tccgaagcga tcagtcaaaa aagccgttta
    25021 ccccaaatga gtaggctctc ttctgcccgc cctgaaggga gtccatcgga atcaccattc
    25081 cggtatcaac gggtccggtc atcgtgcggg tgtcccccgt gccgatatca tagggaatcc
    25141 cgactttggc ggggcagtag atcccgatca catcatcctt ctttgccgtg accggcgtgt
    25201 caaaactgac tttgaacacg ccttcggccg gaatattgaa caccttgctc atccagacca
    25261 cagccagttt gttgtcctgc gggcggagca ggcagaatac cgcgcgtgtt cccgaactgc
    25321 cgtagagccg tccgccgatc agggttgcgc cgcctaacag cgcgttctgc atgtcgacca
    25381 gaagaagatt ttccccgccg gttttatccc ttgcaatcag ctctcctccg acagaatcaa
    25441 aaagttcggc attctgcgaa aaatcccgca gggattccac ctgaagctgg acaaacgagc
    25501 ggatttgaga ccggaccgcc gcggtttcct caacgcgaac gcctttttct ttttgaaggg
    25561 tgctgttgat cttcttgagc gcggccagct gctgctgcag tttctccgcg gcggcctttt
    25621 ccgcggccag ctgcgattcc agcgccgcgt ttttccgctg cagcgatgcc gcttttcctg
    25681 ttgcacagcc ggaggccgct gccagcagca tgcccagcat gacagaaaag acaagccgca
    25741 tgatttcctc ctttcggggc attgccccgc attcgatcat ctgttcacct tgctggacgc
    25801 tccctgaagc gggcgccctt caacagtgcc attctatgga aaacaggcgc cgggggcaaa
    25861 cgctaattgc tgaataaaga gacaggttgc agaattgatg aaaatgaata tagtcgcgtc
    25921 taaaaagggg aaggataaat ggttgaagta tttacaggct ggcatccggt gctgcaggcg
    25981 tttgcgggca cctgcttcac gtgggcgctg accgcagcgg gcgctgccgc ggtgtttctt
    26041 cacagggagc ccagtcagcg ttcacaggac ctgatgctgg gatttgcggc aggcgtgatg
    26101 attgccgcaa gctactggtc gctgctcgct ccggccattt ccatgtcgga gcatctcggc
    26161 tggctgaaat ggtttcccgc cacgacgggg tttctgctgg gcggtgtgtt cctgcgcctg
    26221 attgaccgca ttctcccgca catccatccg gaatcacaaa tggaagacgg catgcacgcg
    26281 acatggaagc gggccgtgat gctggtgacg gcgatcacgc tgcacaattt ccccgaaggt
    26341 cttgccgtcg gcgtggcctt tggggcggcc gccgccggcg actcggccta caccatcggc
    26401 gcggccatcg cgctggcggt gggcatcggc atccagaacc tgcctgaagg cattgcggtt
    26461 tcggtgccgc tgcgccggac cgggatgcgc gcgcgcaaga gtttctatta cggccagctt
    26521 tcgggaatgg tcgagcccgt tgccggcgtg ctgggcgcct tggccatcat ggcgatgaag
    26581 ccgctgctgc cctacacgct ggccttcgcc gccggcgcga tgattttcgt ggtggtcgag
    26641 gatgccattc cggaagccca gagccacggc cacaacgatc tggtgaccat gggcgcgatg
    26701 atcggttttg cgatcatgat gacgctggac gtggcgctgg gctgatcgcc gttcaagtcc
    26761 cgacaatcta gtccaccacc agcgcttccg cggctcctcg cgccggttcg gtcggcggct
    26821 gccgccccat caggtcaatg cttttccacc ggtcccggcg gaaccggagg atgtagccca
    26881 ggcccagcac cgcaatatag aacgcagtcc acagccagca ccagatgatg cttcccttca
    26941 gcacattgac aatcagcagc tgcccgggca ccagcagaag ccaggccatg gtgacggaat
    27001 agtacatcac aaagtccgtg tcccccgcgc ctttcagcgc gtggccgatg atcaggttgc
    27061 tggcatccat aatgccccac agcggaagga tcaggagcag aatccgcccg acgggaagca
    27121 ggtcagagag ggggatgccg ctggtctccc ggttgacaaa gagcgaataa tagaattgcg
    27181 gaaacagcac aaatgtgatg ccggtcagaa gcatgtagag catgccgatc tgccatgcgt
    27241 tccaggcggc cctttccgcg tgctccggct cgcgccggcc gatgtactga ccgaccaga
//
LOCUS       DAOQWR010000017        26753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2122_length_26753_cov_4.682336, whole genome shotgun sequence.
ACCESSION   DAOQWR010000017 DAOQWR010000000
VERSION     DAOQWR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 26753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26753
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_26753_cov_4.682336"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKK36_02615"
     CDS             complement(<1..369)
                     /locus_tag="PKK36_02615"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNR93481.1"
                     /translation="MSAKAPHDKNSSSVAENLRPLLFEAAWEVCQKIGGIYTVIRSKV
                     PEIMSTWGQRYCLLGPYNPEVSPLEFEETELDGPLAPVIAKMREKGFVVHTGYWLVTG
                     RPRVILFEYKQFESRLPEIRK"
     gene            complement(536..3187)
                     /locus_tag="PKK36_02620"
     CDS             complement(536..3187)
                     /locus_tag="PKK36_02620"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNR93482.1"
                     /translation="MKSSTVLLFSACFLYSAVSAFGGPSLPVTKPPVADQSILTGPPV
                     DPAATNGQWFLDYASGVEHYRTHEFHDNLFGGLMGVNAITGFVSSITYLAGPGSPITA
                     FSIQATVWNDNTPVGEYMGGTNSHGESLLYIEAPYTDTLYNVKLTASFAVTDTTNLPS
                     PSPIGPYPPYLDQQPYIEATNEDQAAWYCWSPESVESPTGGYFVPTWDFGDILPGQSS
                     TRQLDFIVPATMSPIDFRYSVLVQSQATQADILLNRSTSLKISTWMDTIYFDTGAPYP
                     EEPFRGSDVSVFHSIPEEEEEPEQDFGDAPDPNYPTLLVNDGARHIIVPGIQMGSAID
                     AEADGQQSPNADGDDNAGIADEDGVSFMGLNYAGQISTVAVSCSTSGWLYIWVDFDAN
                     GSWGDFGEHQNQWATQGLNYAQIAFPTNAAIGNTCARFRFTTDTNSLNNTGQARDGEV
                     EDYLITIKKEEEEQLDFGDAMDNALMPVYPTLAANAGACHTIVPGVMLGTAVDGEPDG
                     QPAPNADGDDNNPPPPGGIDDEDGVTLPSVFVAGASPVISVVASTGGFLDAWIDWNCD
                     NDWADPGEQVFTSTPLSAGLNVLTLLVPQPPVLAAGGPHSRWRFTTNATASVSYTGWI
                     DHGEVEDYEVRLEVLDFGDAPDPSYPTLLASDGARHRVPSVYYLGATAPDSDPDGLPA
                     PSADGDDLSNLPDEDGVSLGSALFRGQTTMSTVSASIGGLLDAWMDFNADGDWSDAGE
                     NIAASHPLSAGANPFNITVPASAALGPTYARFRFSSGGGLSYTGLASDGEVEDYMFTI
                     HQQSPDTNTFAVTSMVLSAGSVFTIQWAGESNVTYETQTLGDLLTTAAPPWTAWGPRI
                     TGGPYTLTFTNILETTRFFRVVAPFAQ"
     gene            complement(3264..4385)
                     /locus_tag="PKK36_02625"
     CDS             complement(3264..4385)
                     /locus_tag="PKK36_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNR93483.1"
                     /translation="MDQIHDFFASCIAGTENVLCDELRELKIPSVRLNRGGIPFRGEW
                     RDGWRACLESRIAQRIQLLLARFPCPDENALYRGIREIAWQEYLTPQKSFAVSAVLRA
                     SKLTHSGFAALKVKDAIVDHIRDLENKRPSVDRDDPDVRVFLYMANDKASVYLDMSGE
                     SLHIRGYRTAAGEAPLRETLAAALLRMSGWDRKTALLDPMCGSGTLAIEAAQWAINKA
                     PGLERRQFGFERWANFTSEMAHALRVLKGELRGAISGTSPKIQAADIDSAMVEIARKN
                     ARTAGVRLSFKQRSLFETQFDGGRQLVIFNPPYGERLEADQEFIRKTAAAVSRMHGWR
                     VCILAGSPEYQRMIPAKPEAVISLKNGDIPCDFLVYEID"
     gene            complement(4415..5572)
                     /gene="arsB"
                     /locus_tag="PKK36_02630"
     CDS             complement(4415..5572)
                     /gene="arsB"
                     /locus_tag="PKK36_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNR93484.1"
                     /translation="MTSIFERYLTVWVLLCIAGGIILGKVAPHLAKTLDGMAIYVQGA
                     PVVSIPIAICLFFMMYPIMVKIDFASVVKAGKSGKPVWLTLFVNWAIKPFTMYGIALL
                     FLGVLFRGFIGADAVDLVKMPFGLDLPVGAEHGAGVVILQDGIKMLQVPLWRSYLAGC
                     ILLGIAPCTAMVLVWGYLAKGNDGLTLVMVAINSLTMLALYGVLGGFLLGVGRLPVPW
                     QALVLSIGIYVALPLVAGYFSRRWIIASKGETWFKEKFLHVLTPVTIIALLITLVLLF
                     SFKGEVILNNPLTILWIAIPLFIQTNLIFALGYWMARLLKLSYEDAAPAAMIGASNHF
                     EVAIATATMLFGLSSGAALATVVGVLIEVPVMLMLVRICTSTQKWFAKETL"
     gene            complement(5587..6357)
                     /locus_tag="PKK36_02635"
     CDS             complement(5587..6357)
                     /locus_tag="PKK36_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNR93485.1"
                     /translation="MSSRNKDKIRQNVRGRYAGIAEGKSCSCCSAPQESAKLGYTEQD
                     LRDVPEGSDMGLGCGNPQAIAALRPGETVLDLGCGGGFDCFLAARQVGDSGLVIGIDM
                     TAEMISKARANAEKCGARNVSFRLGEIEYLPVADSSVDVIISNCVINLSPDKEQVFAE
                     AFRVLKPGGRLAISDVVAVKPLPENTIRNMDQYCGCVSGAMAIAEQERILRKTGFTSV
                     KVTPKPESREFIHTWFPGTGIEDCVLSADITAVKSGGK"
     gene            complement(6460..6888)
                     /locus_tag="PKK36_02640"
     CDS             complement(6460..6888)
                     /locus_tag="PKK36_02640"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNR93486.1"
                     /translation="MKILFLCTGNSCRSQMAEGWARHLKGDVIEAYSAGIETHGLNPL
                     AVKVMNEAGVDIAGHHSKNLSDLADVQFDYVITVCDNAHESCPIFPGKTKVVHAGFDD
                     PPRLAKDAISEAEALAHYRRVRDEIRAFVESLPEKLEIME"
     gene            complement(6885..7250)
                     /locus_tag="PKK36_02645"
     CDS             complement(6885..7250)
                     /locus_tag="PKK36_02645"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR93487.1"
                     /translation="MNLRGAEAVFQALSDKGRLRILCALRHGELCICWIVEMLGFAPS
                     TVSRHLSVLRSAGLIQTRKQERWVYCSLPSMPVSPIIGKILPQIFQSLENSPEVRADA
                     RLIKRILKKEASKCKIRKK"
     gene            complement(7322..7762)
                     /locus_tag="PKK36_02650"
     CDS             complement(7322..7762)
                     /locus_tag="PKK36_02650"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNR93488.1"
                     /translation="MRTMLALLLAVVAGMLLPSPDSCAQSPTNAPSDSGLVMYYFHRT
                     GRCQGCLEIERLTRKTADEFKEEYPLDLFTLEVKNLDEPENERFADDFKVTFNTVVIA
                     SRKDGVVAGWTNLNDRVWNLYSDEGSFVDCIKEELRKRQPDQPE"
     gene            complement(7759..8481)
                     /locus_tag="PKK36_02655"
     CDS             complement(7759..8481)
                     /locus_tag="PKK36_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNR93489.1"
                     /translation="MERTRVCAVIELFTQLSHAIEGTPLIAAGASLLWGVLSILLSPC
                     HLASIPLIVGFIDEQGSISTKRAFQISLLFSVGILITIALIGVLTALAGRMMGDVGRY
                     GNWFVAAVFFVVGLHLMEVIPLKFTAPEQVNMKRRGYLAALILGLVFGVALGPCTFAY
                     MAPMLAITFKMSATRWLYGAFLLLMYGLGHCGVIVLAGTSYEWIEHYLNWNEKSKGAV
                     ILKKLCGLLVILGGLWLIWSAP"
     gene            complement(8454..8852)
                     /locus_tag="PKK36_02660"
     CDS             complement(8454..8852)
                     /locus_tag="PKK36_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR93490.1"
                     /translation="MKTSARIIIVVLLAAAVAGVIMARQNRTVPESADTTPPVSALPR
                     LVDLGADKCIPCKMMAPILDELKETYAGKLEVEFIDVWKNETAGQEYGIQMIPTQIFF
                     SPDGEELFRHEGFFSLDEILAKWKELGYAL"
     gene            complement(8856..9089)
                     /locus_tag="PKK36_02665"
     CDS             complement(8856..9089)
                     /locus_tag="PKK36_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR93491.1"
                     /translation="MKLIQILGTGCQKCKQLYANTEAAVKALGIEARIEKVEKIPEIM
                     KYGVMTTPALVVDGKVKSAGKLLAPEDIQKLLN"
     gene            complement(9126..10511)
                     /locus_tag="PKK36_02670"
     CDS             complement(9126..10511)
                     /locus_tag="PKK36_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNR93492.1"
                     /translation="MDWRTESKKLALILGVFLAIFYLPVGYARFDGAVMEALHLVKWY
                     AREHVLLCLVPAFFIAGAIGVFVSQNAVMKYFGPKARKLLSYGVASVSGTILAVCSCT
                     VLPIFGGIYMRGAGLGPAIAFLYSGPAINVLAIVLTARVLGWQLGLARAVGAVAFSIL
                     IGLLMHLIFLKEERARHAEPQDVFIGDDDKPPRPLWQTALYFFSMAAVLVFANWAKPS
                     EATGVWHAIFASKWIITAVFGLLLVFTLWKFMGVKPRHLAAGIVAVTAAVLLAPDHPV
                     VAFTAGCIALAVITSVSTREMKDWRDQTWGFAKQITPLLLGGVLAAGFFLGSPESSDA
                     GIIPSRWIQALVGDSPSVFWSYAAGSDAAVPGWLSFIWPLWTSFFASITGALMYFATL
                     TEVPILRGLMDSGMGQGPALALLLAGPALSLPNMLVINSIIGPKKTLVYVVLVVVMAT
                     ATGMLFGAVAG"
     gene            complement(10514..10813)
                     /locus_tag="PKK36_02675"
     CDS             complement(10514..10813)
                     /locus_tag="PKK36_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR93493.1"
                     /translation="MNNKRKSRLDGRAKIMKALAHPARLLIVEELSGGERCVCEFVDM
                     IGGDFSTVSKHLSVLKKAGLVMDRKSGLQVYYSLRCPCVLKFFDCLADVIAEQKG"
     gene            10823..11092
                     /locus_tag="PKK36_02680"
     CDS             10823..11092
                     /locus_tag="PKK36_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93494.1"
                     /translation="MHYLAKMSNTSGGIPPVKKTAEKSKKTTKKCHKTTKKCPKRPKM
                     LKNSLFSLIFRNIRQEAIAAIGTNVFVPGANIRLDARGCFYRLRP"
     gene            11103..12089
                     /locus_tag="PKK36_02685"
     CDS             11103..12089
                     /locus_tag="PKK36_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93495.1"
                     /translation="MFRWLMMIAGSVLLALAAMMFHKYPPLPLTPETLSVQQAEILLS
                     EDDNHYVRMSVQLDTDHVIYPTASVRPVYTGRPTDQRYPLDLAAGLPSDALAFLGTVV
                     HITKPLEAEYLRLQILSRKKGKADEVLRERLLAPVRGSGLSLWALSPVFKADEAAGKS
                     WLRAQEHEGPLTRLAELKKNMPWPELEHDLKDILAVAEKEYGQEIPEDALLLITDYSW
                     DFKPLRYYPVAEGMGGRLFVMLPHEVLPGTEPFDVTGILDPDPSHLYDDFATVLDVPV
                     PARIGIITTESVETYNRRKAGSAKGTAATGLTLLLAGLGGTGLRKLFRKNRG"
     gene            complement(12097..12567)
                     /locus_tag="PKK36_02690"
     CDS             complement(12097..12567)
                     /locus_tag="PKK36_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93496.1"
                     /translation="MNRVWLFVVTALLAGCGGSKDKSMLLKDWDSVFVPLGIETLTSM
                     DDALKEKGIGNVKSSDMKLVFTYMDSEFSDGIGPGAIVYSDIYAVPPRTMFMEMRVWQ
                     CYEAILNDPRAKGIHVNPDAPNKKPYTVSATEMRGALDKLPRQPRMPMDISIKN"
     gene            complement(12570..13472)
                     /locus_tag="PKK36_02695"
     CDS             complement(12570..13472)
                     /locus_tag="PKK36_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNR93497.1"
                     /translation="MHIPQAMLQGTICPVTAAASAAGIAGAAFAAVKSKFKPVAGRFA
                     AVTALIFAAQMINFPISGGTSGHLLGGVLASSLLGTPLGMLSIALVVAVQALVFSDGG
                     LTVLGANIFNMSIVGTGLGGLIHSTLSAKNNTRIFTAASAGIAAWISVVAASFAVSVE
                     LSVAGTIPFSTVIAAMISTHAVIGIGEGLITAAAVYALASRPAEEKVRGSVAIPLSAA
                     AVLGLMISPFASSLPDGLEWVAEKYSFLHESAPAFVAVMPDYTIPVISFEMLSTGLAG
                     LAGVVICFGIAWISAQTLLARARR"
     gene            complement(13550..14821)
                     /locus_tag="PKK36_02700"
     CDS             complement(13550..14821)
                     /locus_tag="PKK36_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNR93498.1"
                     /translation="MAAAQKGELAKIEELMDAGADPHACSKYSESPFGRAARAGHTEA
                     AHLMLDKGGVTQNPEKQNCPTPLMMAAMTDCTNLIARLLEAGADVNEKRIGGRTPLIL
                     AAQNGHNEAVRMLLDNGADMNVTVGLDNMTVVQWAIFTGKKDTVELLLDRGADPGPTP
                     LMGPACQGNLEMMEVLVSRGADVNARRLAATSVRNNQEWEAALHAAATCCRTDSMAWL
                     LEHGAPVDDPGAYGKTPLMSAITDRCHDGALLLIEKGANVNAVDRLNLTPLIYAARGG
                     DPNVIQMLLKHGADVNFKGKSGMTALDAALEWEHDHIAVLLIKSGADVTADTLLRAAQ
                     ERSAAWDNGELMQSILDRGVDVNSTSDLGETALMAAVQKGLTDNVQWLLSRGADATLK
                     DNSGKTAEDYTKDPKILEALRRSREENVSTE"
     gene            complement(15078..15410)
                     /locus_tag="PKK36_02705"
     CDS             complement(15078..15410)
                     /locus_tag="PKK36_02705"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNR93499.1"
                     /translation="MKKALCFILLSLFTGCSSLITQFGTDKDDMYECGPPVKVKQIYS
                     GTACNILHAKAVINGTTGMDDPGRAIEVCIFPFYYVFEFPLSLVMDTVLLPMTICKQI
                     IYGDLTID"
     gene            complement(15416..16483)
                     /gene="ychF"
                     /locus_tag="PKK36_02710"
     CDS             complement(15416..16483)
                     /gene="ychF"
                     /locus_tag="PKK36_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNR93500.1"
                     /translation="MNLGIIGLPQTGKKTVFELLTGIHAQKAVTKDGIAYAMAAVRDP
                     RVDKLSAMYKPKRTRYAEFEIALPPDIQPDASRNAGWIEPLRRVDALLHVVRAFEAPE
                     VFHIKGDVNPARDYDLVETELLLADLGTVETRLTRMAKEIKKKGDTTSDREEAVLKRC
                     QQHLETGAPLLTMQFSDEELKLVASLKFLTLKPVITVFNTGENISKAREALKDTETRI
                     KKHGAEPVYLSASIEKEMLELSEEERAMFMADLGIEEPAAHRLSRAVYASLGLISFFT
                     VGPDEVKAWPLRAGTRAPQAAGKIHTDLERGFIRAVTISYDHLIEAGSEHTAKEKNYY
                     VLNGKDYVVKDGDVIEIRFNV"
     gene            complement(16531..16929)
                     /locus_tag="PKK36_02715"
     CDS             complement(16531..16929)
                     /locus_tag="PKK36_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93501.1"
                     /translation="MKIKRLWWLWTALLTGAVAVGQDIMPPGGDASPLPLVTHDEQGR
                     MIRHVIFDPDGNVLQMTTYEYIDKDITLQTITVIHGASGWTVTESLSMADTGVRLNSV
                     REYDAEGELMDEKLVVIHPGSRPFRIPEDL"
     gene            complement(17140..17523)
                     /locus_tag="PKK36_02720"
     CDS             complement(17140..17523)
                     /locus_tag="PKK36_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93502.1"
                     /translation="MKFTALAGGLMIMLASGCATRGPRSILPPPLITYDGQGRILRQV
                     QYNPDHSVNTVKAYQYPDGGVTLETVTVYDSTNGWTVTETYNMTDSAVGLKNVKQYDA
                     GGNLLSDRNALVNPGSRSVRINNSP"
     gene            17618..17968
                     /locus_tag="PKK36_02725"
     CDS             17618..17968
                     /locus_tag="PKK36_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNR93503.1"
                     /translation="MEALIRDIKQFAQERDWEQYHSPRNLIMALSVEVAEVMEHFLWL
                     TGEQSRNLPEKKKNVIMDEIGDVQILLAHLADQMGIDPIEAARQKLVKNRAKYPADKV
                     RGKALKYTEYLSVE"
     gene            17968..18588
                     /locus_tag="PKK36_02730"
     CDS             17968..18588
                     /locus_tag="PKK36_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR93504.1"
                     /translation="MDFVAFDTETTGISPHRDAVIEIGAVRFSDGIPGESFSELIDPG
                     RPIPRGATRIHGITDEMVSSSPQIGEVLPRFAEFCGDLPLVAHNARFDFKFINAAVRR
                     LACAAPTGLVLDTLTLSRKLWPGLPNYQLGTLVRVFQVPCGRFHRAAEDALYCGHVFQ
                     ILLERCRINWPDMDADGLVKICGRRKMRFMQSAEDRAATWFADFFN"
     gene            18604..20658
                     /locus_tag="PKK36_02735"
     CDS             18604..20658
                     /locus_tag="PKK36_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93505.1"
                     /translation="MTYKKANAALTAVCAIPLAAMLLAGCAGLGTESAGYIHHRMESG
                     TTPPAGEAVSVRGIVHQVLVFSSKSTPSYYGFVVQNPADAADRDPSTSDGLYVDMHTN
                     GFLRTSSGGIYRPHVGDDVLLKGVMGTSAGQTVLQQAEFRKLMRSRLNIDSELKAFVA
                     NPPDDAGEAEIYWRRHEGMRCKVPAGAVVSGAHRDSMGSMVYLIHPSHPLLRRENNYA
                     RRAFRDPHPLDDREEIFDNQNGYLIGLGPLGLDRADGDPMPLFPETCTFEVLKEPVMG
                     AVARFMGQYMVQVDSLPVFEVGTDPSANRSDADAGDESVLRIATFNVENLYDHRDDPF
                     DDRDYFDRKDALPILDADGKFQGYSGLKLYDYVSASKDAYKARLSGLAGQIIEDLQAP
                     DILMLQEVEDQDIGAVEEGKLVVGDINNRDGKLDVLQELALEISRLGGPAYEPVSDRA
                     AADDRGIITAFLYRPDRVALAEISEDHPVMGVMPCVPSAGRTMTYCGRAGNPKAFNLL
                     HDILGVVHERGPQMAAFDFGAGEQARRIYLLNNHFKSQPQNYVPERQNQAQFSADLVR
                     ALRKDDKKVLAAVGGDLNTFPRPDEPDPMLPTDQLGGLYAADMVNLYDEMLKTRPENA
                     YSYVFKGQAQTLDHLFVTESLHKKLKHVNAVHINSDFAGSEKYPRRGQSDHDPILAGF
                     QF"
     gene            complement(20689..21108)
                     /locus_tag="PKK36_02740"
     CDS             complement(20689..21108)
                     /locus_tag="PKK36_02740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNR93506.1"
                     /translation="MEALKAKLGEKSADLVYPPPVYTAMKGEMLDFDLGAGILTNRFP
                     VLREYLNPYGSLQGGIIATLLDDTIGPLSLLVAPANVTRNLEVKFRRPVTPDLGAVTV
                     TARFTGQNDRQLFFEAEMKSGDGTIRAQARAVHWILA"
     gene            21208..22560
                     /gene="trkA"
                     /locus_tag="PKK36_02745"
     CDS             21208..22560
                     /gene="trkA"
                     /locus_tag="PKK36_02745"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNR93507.1"
                     /translation="MKIVIIGAGNAGTQLAERLCEERHSVVMIDERMDVLMQAEASLD
                     VLTLCGSGSDPVLLERAGTDKADLLIAVTDRDEINIIACLLAHSVGVPRVVARVGSAQ
                     YLRSSSKYNLKNMGINLVINQKQACAEEICSALALPGAIESFDLFEGRVMVAGFNISA
                     DSPILGLTPATLPDQALVRKLRIMAVRRKEKLIIPRGDTSFAPQDSIYLVGSPDDIRT
                     FSIWMNPSQTPFMKVIVAGGGDIGLTIAQTLEEHSDAEIVMLEQDEDRALFCSGELKH
                     TLVLRADALSGSALEETGIVPRTAFVGMTGDDENNVMNCLMARKKGADYTITQIARTD
                     YIPVVESLGLVDRIISPFVSTTRGILHYLRSRNVRAATLLHNLPGELLDIIVMPGNKY
                     IGQAVQDIKFPREAVLAVALRGNEILPATGDLSIMADDRLLVFADSSAVKKIQTMFFK
                     "
     gene            22571..24022
                     /locus_tag="PKK36_02750"
     CDS             22571..24022
                     /locus_tag="PKK36_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNR93508.1"
                     /translation="MNKRAVFHLVAYMTLVIGLAITACAAVSWYYGDTFTVINAFIHS
                     GVITIAVSGLVGVLTHGDINLSRRDGFGVVTIGWLSAALFGALPYVFSGVILHPVSAV
                     FETISGFTTTGASVLSDLEALPRGILFWRALTHWFGGMGVLVLCVAILPFLGVGGMQL
                     YRAEMPGPSKDRLTPRITTTAKLLWGVYALLTVFEALLLKYIGKMDWFDSLCHTFATM
                     ATGGFSTRTASVGAYNSAAVDLIILFFMFMAGINFSLHYYALTGRPGRYFRDPECRAY
                     FLLLAGCGLFITFNIWAGGYAAFTRCLRDAFFTSASILTTTGFCTADFDRWPDASRLL
                     LVLMMFVGGCAGSTGGGIKIVRVMIMVKKIIRETKLFMRPSAVLQLKLGKNSVDQEII
                     SHISAFFTIFILIFGLCSFLMSFFTPNLETATTAVIAALGNIGPGLNAVGASQNYAAI
                     PAAGQGILIVCMLLGRLELYTVLILFFPGFWKR"
     gene            24274..>26753
                     /locus_tag="PKK36_02755"
     CDS             24274..>26753
                     /locus_tag="PKK36_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNR93509.1"
                     /translation="MQDFEKLGAFYLGNIYDRAAGKRTEDILLYDSKDLTTHAVCVGM
                     TGSGKTGLCIGLLEEAAIDGIPAIVIDPKGDIGNLLLTFPNLQPADFLPWVDAAEAAR
                     KNMTPEQFAEKTADTWKNGLASWGQDASRIAMLKDKADMAIYTPGSDAGLPLSIVKSF
                     AAPPESLRGDQDAMRDRIMAAVSGLLALLGVDADPIQSREHILLSNIFSAVWQEGRDL
                     GLPDLIRAIQDPPFKSVGVFDLESFYSSKDRMTLAMRLNNMLASPGFSAWMNGEPMDI
                     QRLLYAPAGKPRISILSIAHLNDSERMFFVTMLLNEVISWMRAQPGTSSLRAILYMDE
                     IFGYFPPTANPPSKTPMLTLLKQARAYGLGCVLATQNPVDLDYKGLSNTGTWFIGRLQ
                     TERDKMRVLDGLEGASGSAGKSFNRAEMEQILAGLGQRVFLMNNVHDDAPVLFETRWV
                     MSYLRGPMTRAQIQQLMDQRKSAAEQPASVMASAGPVATASTRPVVAPGITELFLAAS
                     KMPVDGSSVVLRPALLANVKMHFTNAKTKTDEWQTRCLMAEIPENAGMMDWEASDILA
                     SEPQFSSGAVAGAKYADLPASAGQAASYKSWEKTLKSYVYESIVLNLFTCPALKEVSK
                     PEEEEGDFRGRLAQSAREHRDAALEKVNKTYGSKMTTLQNRIRAAEAKIEREKAQYKD
                     QKLKTFLSMGATVLGAMLGRKAASVSTVGRAATTMRSAGKISSQKGDVAHAETNLEAL
                     NQQYAALDAEYQAELEKIIIEKLQTIYDPEIPVNIYDLGFIYEIRIDDNYQVTIKMTL
                     TSPNCPVAETLPIDVRDGIQAISGIKSV"
BASE COUNT         5704 a         7778 c         7600 g         5671 t
ORIGIN      
        1 cttgcggatt tccggcaggc gcgattcaaa ctgcttgtat tcaaacagga tcactcgcgg
       61 ccggccggtc accagccagt agccggtatg caccacgaac cccttttcgc gcattttggc
      121 gatcaccggg gcgagagggc cgtccagttc tgtttcctcg aattccagcg gcgatacctc
      181 gggattatag gggcccagca ggcagtaacg ctgcccccag gtggacatga tctccgggac
      241 cttgcttcga atcacggtat aaatgccccc gattttctgg cagacttccc atgccgcctc
      301 aaaaagaagc ggccgcagat tttccgcaac cgatgacgaa ttcttgtcgt gtggcgcttt
      361 cgcactcatg tctcgacccc tttcaaaact tccaatggct ggaacttttt gcaaaaaaac
      421 ttccaatcat tggaactttt cgcgcagaaa cttccaatga ttggaacttt tgaaatacca
      481 tgccgccaaa ttaagcgaag cggccgcgca agtcgagcag gaagcttgat taagcctact
      541 gggcgaaggg cgccacgacg cggaagaacc gggtcgtttc cagaatattg gtgaacgtca
      601 gcgtataggg tccgccggtg atgcggggcc cccatgccgt ccagggcggc gcggccgtcg
      661 tcagcagatc gcccagcgtc tgtgtttcat aggtcacgtt gctttcgccc gcccattgga
      721 tggtgaaaac gctgcccgcc gaaagcacca tgctggtgac ggcgaaggtg ttggtgtctg
      781 gtgactgctg gtggatggta aacatgtaat cctccacctc tccgtctgaa gccagcccgg
      841 tataactgag gccgccgccg ctgctgaagc ggaaacgcgc ataggtcggg cccagcgcag
      901 cggatgcggg gacggtgatg ttgaagggat ttgcgcccgc ggacagcgga tgggatgccg
      961 ctatgttttc accggcgtcg gaccagtctc cgtccgcatt gaagtccatc catgcatcca
     1021 gcaggccgcc aatcgacgcg gaaacagtgg acatggttgt ctgcccgcga aacagggccg
     1081 acccgagaga aaccccgtct tcgtccggca gattggacag atcatccccg tctgcgctgg
     1141 gggcggggag tccgtccgga tcgctgtcgg gcgccgtcgc ccccaggtaa tagaccgaag
     1201 gaacccgatg gcgcgccccg tcgcttgcga gaagagtggg ataggacggg tccggcgcgt
     1261 cgccgaaatc gaggacttcg agacgaactt catagtcttc cacttcgcca tggtcaatcc
     1321 atcccgtgta cgaaaccgag gcggtcgcgt tcgtggtgaa ccgccatcgg gaatgcgggc
     1381 cgccggcagc cagcacgggg ggctgcggca caagcagggt caggacgttg agtcctgcgc
     1441 tgagcggtgt gcttgtgaac acttgctccc ccggatcggc ccagtcgttg tcgcagttcc
     1501 agtcaatcca cgcgtcaaga aaacctccgg tggaagccac aacggaaatg accggactgg
     1561 caccagcaac aaaaaccgac ggcagcgtca cgccgtcctc gtcatcaatg ccccccggag
     1621 gaggcgggtt gttgtcgtct ccgtcggcgt ttggcgctgg ctgcccgtcc ggctccccgt
     1681 ccacagcggt tccgagcatc acccccggca caatggtgtg acacgcgccg gcgttcgctg
     1741 cgagcgtcgg atagacaggc atcagcgcat tgtccatcgc gtcgccgaaa tcgagctgct
     1801 cttcttcctc ttttttaatc gtgatgagat agtcttccac ttctccgtcg cgggcctggc
     1861 cggtgttgtt caatgaattg gtgtcggtgg tgaaacggaa gcgggcacag gtgtttccga
     1921 tggcggcgtt ggtggggaag gcgatttgag cataattcag accctgtgtg gcccactggt
     1981 tctgatgttc gccgaaatca ccccagctgc cgttggcgtc aaagtccacc cagatgtaga
     2041 gccagcctga tgtggagcag ctcaccgcca cggtgctgat ctgtccagca taattcagcc
     2101 ccatgaagct gaccccgtct tcgtcggcaa tacccgcatt gtcatcgccg tccgcattgg
     2161 gagactgctg tccatcggct tcagcgtcaa tggcggatcc catttgtatc cccgggacga
     2221 tgatgtgccg cgcgccgtca ttaaccagca gtgtcggata gttgggatcc ggcgcatcgc
     2281 cgaaatcctg ttccggctct tcctcctctt ccgggatgct gtgaaacacc gacacatcgc
     2341 tcccgcggaa gggttcttcc ggataagggg ccccagtatc gaagtatatg gtatccatcc
     2401 aggtgctgat tttcagcgag gtgctccggt tcagcagaat atccgcctgc gtggcctgcg
     2461 actgcacaag cacgctgtag cggaaatcta tcggggacat cgtggcggga acaataaagt
     2521 caagctgccg ggtcgaggac tgtccgggga ggatgtcgcc aaagtcccag gtcggcacga
     2581 aataaccgcc ggtcggagac tcaacacttt ccggtgacca gcagtaccat gcggcctggt
     2641 cttcattcgt ggcttcaatg tacggctgct gatccagata cggcggatac ggcccgatgg
     2701 gagacggaga aggcaggttg gtcgtgtctg tgaccgcaaa cgaggccgtc agcttcacgt
     2761 tgtacagtgt gtccgtgtaa ggagcttcta tatacagaag tgattcgcca tggctgttcg
     2821 tgccgcccat atattccccg acgggggtgt tgtcattcca gaccgttgcc tgaatgctga
     2881 atgccgtgat cggcgaaccg ggtccggcca gataggtaat gctgcttaca aatcccgtga
     2941 tggcattgac ccccatcagg ccgccgaaca ggttgtcgtg gaactcatgt gtgcgataat
     3001 gttcaacacc gctggcataa tcgaggaacc actgcccgtt cgtggccgct ggatcaaccg
     3061 gaggcccggt cagtatactt tgatccgcga cgggcggctt ggtgactgga agtgaaggcc
     3121 ccccgaatgc tgaaaccgcc gagtacagaa aacatgccga aaagagaaga acagtactgc
     3181 ttttcatgac acaacctccc cgcgaaacgc accccaaact gcatcccgct ctatttgcag
     3241 tttctccccg tcggggcttt gtgtcaatca atttcgtaca caagaaaatc gcagggaata
     3301 tccccgtttt tcagggagat tacggcctcg ggtttggccg gaatcatgcg ctggtattca
     3361 ggacttccgg caaggatgca gaccctccag ccgtgcatcc ggctgaccgc cgcggctgtt
     3421 ttgcggatga actcctgatc cgcctccagc cgttcgccgt agggcggatt gaaaatgacc
     3481 agttgccgtc cgccgtcaaa ctgggtttca aacagcgacc gctgcttaaa ggaaagcctg
     3541 acgcctgctg tgcgggcatt tttccgggcg atttcaacca tggcggagtc aatgtccgcg
     3601 gcctgaattt tcgggctggt gcctgagatg gcgccgcgca gttcgccctt caaaacgcga
     3661 agggcatgag ccatttcaga ggtaaaattt gcccaccgtt caaacccgaa ctgcctgcgc
     3721 tccagacccg gggccttgtt gatcgcccac tgcgccgctt caatggcaag ggttcctgaa
     3781 ccgcacatgg gatccagaag ggctgtttta cggtcccagc cggacattcg caggagagcc
     3841 gctgcaagag tctcgcgcag cggcgcttcg ccggcagccg tgcgatatcc gcgaatatga
     3901 agcgattcgc ccgacatatc gagataaacg gaggccttgt cattggccat gtacagaaag
     3961 acgcggacat caggatcatc ccggtctaca gacggacgct tgttttcaag atcgcggatg
     4021 tggtccacaa tcgcgtcctt gaccttgagc gcggcgaatc cactgtgcgt caacttgctg
     4081 gcccgcagca ccgcgctcac tgcaaaggat ttctgcgggg tgaggtactc ctgccatgcg
     4141 atttcacgga tgcccctgta gagggcgttc tcgtcggggc aggggaaccg cgccagaaga
     4201 agctggatgc gctgggcgat gcggctctca aggcaggccc gccagccgtc gcgccattcg
     4261 ccccgaaaag gtatgccgcc ccggttcagg cggacactcg ggattttcag ctcgcgcagc
     4321 tcatcgcaaa gcacattttc cgtgccggcg atgcaggacg caaagaaatc atgaatctga
     4381 tccatgacgc atccctagca taaagagtgc cgcctcacaa agtttctttt gcaaaccact
     4441 tctgagtgga tgtgcagatg cgcaccagca tcagcatgac cggcacttcg atcagcactc
     4501 caaccaccgt ggccagcgcc gcacctgatg acagtccgaa aagcatggtc gcggtggcaa
     4561 tggcaacttc gaagtgattg gaggctccga tcatggccgc cggcgcggcg tcttcatagg
     4621 acagtttcag cagccgcgcc atccagtagc ccagtgcaaa gatcagattc gtctggatga
     4681 aaagaggaat cgctatccac aaaatggtca gaggattgtt caggatcact tcgcccttga
     4741 agctgaacaa aagcaccagc gtgatcagca gtgcgatgat ggtgaccggg gtcagcacat
     4801 gcaggaactt ctccttgaac catgtttcgc cttttgaggc gattatccat cggcgcgaga
     4861 aatagcccgc caccagcggc agggcaacgt agatgccaat ggacagaaca agcgcctgcc
     4921 acggcacagg aagacgtcca acgcccagca ggaagccgcc cagcaccccg tagagcgcca
     4981 gcatcgtcag cgaattgatg gccaccataa cgagcgtcag gccgtcattg cccttggcca
     5041 gatagcccca caccagcacc atcgccgtgc agggcgcgat gcccagcaga atgcatccag
     5101 ccagatagct tcgccagagc ggaacctgga gcattttaat cccgtcctga aggatcacca
     5161 cgcccgcgcc atgctccgcc ccgaccggca ggtcaagccc gaacggcatt ttgaccagat
     5221 caacggcatc ggcaccgatg aacccgcgga acaggactcc cagaaacagc agggcgatgc
     5281 cgtacatcgt aaacggcttg atggcccagt tcacaaacag cgtcagccag acgggttttc
     5341 cgcttttgcc ggccttcacc accgacgcaa agtcaatctt caccatgatg gggtacatca
     5401 tgaagaacag gcagatcgca atcgggatgg acaccaccgg cgcaccctgg acataaatgg
     5461 ccattccgtc cagagttttg gccaggtggg gggcgacctt gccaagaatg attccgcctg
     5521 ctatgcacag cagcacccaa acggtcagat accgttcaaa aatgcttgtc atttttctgt
     5581 cctgcatcat ttgcctcctg attttacagc ggtaatatct gccgataaaa cgcaatcttc
     5641 aattccggtt cccgggaacc aggtgtggat gaattcccgg ctctccggtt tgggtgtgac
     5701 tttgacgctt gtgaagccgg tttttcttag aatgcgctcc tgttcagcga tggccatggc
     5761 tcccgatacg catccgcagt actgatccat gttgcggatt gtattctcgg gcaggggttt
     5821 gacggccact acatccgaaa tggccagccg cccccccggc ttcagcacgc gaaatgcttc
     5881 cgcaaaaacc tgttccttgt ctggcgaaag attgatcacg cagttggata tgatgacgtc
     5941 cacggatgaa tccgccacgg gcagatattc gatctcgccc agccggaagg aaacatttct
     6001 tgcgccgcat ttctcggcat tggcgcgcgc cttggaaatc atttcggcgg tcatgtctat
     6061 gccgatcacg agtccggaat caccaacctg ccgagcagcg agaaagcagt cgaatcctcc
     6121 gccgcatccg agatccagca cggtttcgcc gggcctgagt gccgctatcg cctgcggatt
     6181 tccgcatcca agtcccatgt cggatccttc gggaacatcg cgaaggtcct gttccgtgta
     6241 gccaagcttt gccgattcct gcggcgcgga gcagcaggaa cagctcttgc cttccgcaat
     6301 gccggcatag cggccgcgta cattctgtct gatcttgtct ttatttcttg aggacatggt
     6361 caaggctcct gtcgggttgg aatattggaa tggtgggaag acgaggatat aaactgcttc
     6421 cggctgattt catggcttct ccattattcc aatattccat cattccatta tttccagttt
     6481 ctccggcagt gattcgacaa acgctctgat ttcatcccgc acgcgccggt aatgggcgag
     6541 ggcttccgcc tcagatattg cgtccttcgc cagcctcggc ggatcgtcga atccggcatg
     6601 caccactttt gtttttcccg gaaagatcgg gcaggactcg tgggcgttgt cgcagacggt
     6661 gatcacataa tcaaattgta cgtcggccag atcagaaagg ttctttgagt ggtgtccggc
     6721 aatatccact ccggcctcat tcatgacctt cacggccagc ggattcagcc cgtgcgtttc
     6781 aatgcccgcg gaatacgcct caatcacatc gcccttcaga tgccgagccc agccctcggc
     6841 catctggctc cggcaggagt ttccggtgca gagaaacagg attttcattt tttccggatt
     6901 ttgcacttcg atgcctcttt tttaaggatt cgtttaatca gccgcgcatc cgcccggact
     6961 tccggtgaat tttccagtga ttggaatatc tgcgggagta tttttccaat gattggagaa
     7021 acgggcatgg agggcagcga gcagtaaacc cagcgctcct gcttgcgggt ttggatcagt
     7081 ccggcgctgc ggagaaccga aaggtgcctg gatacggtgg agggggcgaa gcccagcatt
     7141 tcgacaatcc agcagatgca cagttccccg tggcggagcg cgcacaggat gcgaaggcgg
     7201 cccttgtccg acagggcctg aaaaacagcc tccgccccgc gaagattcat atttctatat
     7261 ttagccatat aacgaaatat agaacagggg acgcagtgcg tcaacgcgaa aaatgaagaa
     7321 attactccgg ctggtccggc tgccgttttc gcagttcttc cttgatgcag tccacaaaac
     7381 tgccttcgtc ggaatacaga ttccagaccc gatcgttcag attggtccaa ccggccacaa
     7441 cgccgtcttt ccggcttgca atgaccaccg tgttgaaggt gaccttgaaa tcatctgcaa
     7501 agcgctcatt ctccggctcg tccaggttct tcacttcgag agtgaaaaga tccagcggat
     7561 attcctcctt gaattcgtcc gccgtttttc gggtcaggcg ctcaatctcc aggcatccct
     7621 ggcagcggcc ggtgcgatgg aaatagtaca tcacgagacc gctgtccgac ggtgcattgg
     7681 ttggagactg cgcacagctg tccggggagg gaagaagcat tcccgcaacg acggcaagga
     7741 gcaaggcaag cattgttctc atggagcact ccaaatcagc cagagcccgc caagaatgac
     7801 cagcaggccg caaagttttt tcaggatgac ggctcccttc gatttctcgt tccagttcag
     7861 ataatgctca atccattcat aagaagttcc cgccagcaca ataactccgc aatggccaag
     7921 gccgtacatc agcagcagga atgcgccata gagccagcgg gtggccgaca ttttgaaggt
     7981 gatcgccagc atcggcgcca tgtaggcaaa cgtgcagggg cccagagcca ctccgaagac
     8041 aaggcccaga attaaggcgg ccagatagcc cctgcgcttc atgttcacct gttccggcgc
     8101 cgtgaacttg agcgggatca cttccatcag atgcagcccg accacaaaga agacagcggc
     8161 tacaaaccag ttcccgtaac gtccaacatc gcccatcatg cggcccgcca gcgccgtcag
     8221 caccccgatc agcgcaatgg tgatcaatat gccgactgaa aagagcagcg aaatctggaa
     8281 ggcgcgtttg gttgatatgc tgccctgttc gtcaatgaac cccacgatca gcgggatgct
     8341 ggcgaggtgg caggggctca gcaggatgct caggacgccc cagagcaggg atgcccctgc
     8401 tgcaatgagc ggggtccctt cgatggcatg gctgagttgt gtgaaaagct caatcacagc
     8461 gcatacccga gttctttcca cttcgccaga atctcgtcga gcgagaagaa tccttcgtgc
     8521 cggaaaagct cttcaccgtc aggagagaaa aatatttgcg tggggatcat ttgaattccg
     8581 tattcctgtc ccgcggtttc atttttccag acatcaatga attccacttc cagtttcccg
     8641 gcataggtct ccttcagctc atcaagaatg ggcgccatca ttttgcacgg gatgcacttg
     8701 tccgcgccca gatccacgag acggggcagg gcggagacgg gcggcgttgt atcagcagac
     8761 tccggcaccg tgcggttctg cctggccatg atgaccccgg cgaccgccgc ggccagcagg
     8821 acgacaatga taattcttgc agaggtcttc atggttcagt tcaaaagttt ctggatgtct
     8881 tccggggcaa gcagtttccc cgcgcttttt actttcccgt ccaccacaag ggccggagtg
     8941 gtcatcacgc cgtacttcat gatttccgga atcttttcga ccttttcgat acgcgcttca
     9001 atgcccagtg ccttaactgc cgcttctgta ttggcgtaca gctgtttgca tttttggcat
     9061 cccgtcccga gaatctggat cagtttcata tgcgcttttc tcccatcatt ccattattcc
     9121 attcctcatc ccgccactgc tccgaacagc attcctgttg cggttgccat caccacaacc
     9181 agcaccacat acaccagcgt tttctttggg ccgatgatgg agttgatgac cagcatgttg
     9241 gggagcgaaa gcgccggccc tgccagaagc agggcaagcg cgggaccctg gcccatgccg
     9301 ctgtccatca ggccgcgcag gatcggaact tcggttagtg ttgcgaaata catcagcgcc
     9361 ccggtgatgg acgcaaagaa actggtccac agcggccaga tgaatgacag ccacccggga
     9421 accgccgcat cggatccggc cgcatagctc cagaatacag aagggctgtc tccgaccagc
     9481 gcctgaatcc agcggctggg gatgatgccc gcatcgctgg attccggact gccgaggaaa
     9541 aatcccgccg ccagcacgcc gcccagcaga aggggcgtga tctgttttgc gaagccccag
     9601 gtctggtcgc gccagtcctt catttcccgt gtgctgaccg atgtgattac ggcaagggcg
     9661 atgcatccgg cggtgaacgc aacaacagga tgatccggag cgaggaggac agcggctgtc
     9721 acggcgacaa ttccggcagc caggtgccgc ggcttgactc ccatgaactt ccacagcgtg
     9781 aaaaccagca ggagaccaaa aacagcggta atgatccact tcgaagcgaa aatcgcatgc
     9841 cagacgcctg tggcttcaga cggtttggcc cagttggcaa acacgagcac ggccgccatg
     9901 ctgaaaaaat aaagtgcggt ctgccaaagc gggcggggcg gcttgtcatc atcgccgatg
     9961 aagacatcct gcggttccgc atgccttgcg cgttcttctt taaggaagat cagatgcatc
    10021 agcagcccga tcaaaatact gaaggctacg gcgccgacag cgcgcgccag gccaagctgc
    10081 cagccgagca ctcgcgcggt cagcacaatc gccagcacgt tgatcgcggg gccggaatag
    10141 agaaaggcaa tggctggtcc aagccccgcg ccgcgcatgt agattccgcc gaagatgggg
    10201 aggaccgtgc aggagcaaac ggctagaatg gttccggaaa ccgaggccac gccgtaggaa
    10261 agaagcttgc gggccttcgg cccgaaatat ttcatcaccg cattctggct tacaaaaacc
    10321 ccgatggcgc cggcgatgaa aaaagccggt accaggcaca aaagcacgtg ctcgcgcgcg
    10381 taccatttca caagatgcag cgcctccatc accgcgccgt caaatcgcgc atagccgaca
    10441 ggaagataga atatagccag gaacactccc agaatcagcg ccagtttctt tgattcagtt
    10501 ctccagtcca tcgtcagcct ttctgttcag caatcacatc ggcgaggcag tcaaaaaact
    10561 tcagcacgca tggacagcgg agagaatagt agacctgcag accgcttttc cggtccatga
    10621 ccagtccggc tttcttcagc acggagagat gcttggacac ggtggaaaaa tcgccgccga
    10681 tcatatccac gaattcacac acgcaccgct cgcctccgga gagttcctca acgatgagca
    10741 ggcgcgcggg gtgggcgagg gccttcatga ttttggcgcg gccgtccagt ctgcttttcc
    10801 gtttattgtt catgcaaata aaatgcacta tttggcgaaa atgtcaaata cttctggcgg
    10861 cattccgccg gtgaagaaaa cagccgaaaa gtctaaaaaa acaacgaaaa aatgccacaa
    10921 aacgaccaaa aagtgcccaa aacggcctaa aatgctgaaa aatagccttt tttcattgat
    10981 ttttcgaaat atccgccaag aagccattgc ggctattggg accaatgttt ttgttccggg
    11041 ggcaaatatc aggctggacg cgcggggctg tttttataga ctgcgcccat gaagaggatt
    11101 ttatgttcag atggttgatg atgattgccg gaagtgttct gctcgcgctg gccgcgatga
    11161 tgtttcacaa gtatccgccg ctgccgttga cgccggagac gctgtcggtg cagcaggcgg
    11221 aaatcctgct cagtgaggat gacaaccatt atgtccgcat gagcgttcag cttgatacgg
    11281 atcatgtgat ttatcccacc gcgtctgttc gcccggttta caccggccgg ccgaccgatc
    11341 agcgctatcc gctggatctc gcggcggggc tgccgtccga tgcgttggcg tttctgggta
    11401 cagttgtgca tatcaccaag ccgctggaag ccgagtatct gcggctgcag attctgagcc
    11461 ggaagaaagg gaaggcagat gaggtgctgc gcgagcgcct gctggcgccc gtccgaggca
    11521 gcggtctttc gctctgggct ttgtcgccgg ttttcaaagc agatgaagcg gcaggaaaat
    11581 catggctccg ggcacaggag catgaaggac cgctgacacg tctggcggaa ctcaagaaaa
    11641 acatgccgtg gcctgaactg gagcacgacc tgaaggatat ccttgcggtg gcggaaaagg
    11701 agtacgggca ggagatcccg gaggatgcgc tgctgctgat cacggattat tcatgggatt
    11761 tcaagcccct ccgctattat cccgtggcgg agggcatggg cggccggctg ttcgtcatgc
    11821 tcccgcacga ggttcttcca ggcacggagc cgtttgacgt gacgggcatt cttgatcctg
    11881 atccctcgca tctgtacgac gacttcgcaa cggtgctgga cgtcccggtg cccgcgcgca
    11941 ttggcatcat caccaccgaa tcggtcgaaa cctacaaccg gcgcaaggcc ggcagcgcaa
    12001 agggcactgc ggcgacaggc ttgacgctgc ttctggccgg gctgggagga accggactta
    12061 gaaaactctt ccggaaaaat cgcggctgaa ccggtcctaa ttcttgatgg agatatccat
    12121 cggcatgcgc ggctgtcttg gaagtttatc cagcgccccg cgcatttcgg tcgcggaaac
    12181 cgtgtagggt ttcttgttgg gcgcgtcggg gttcacatgg atgcctttcg cccgcggatc
    12241 attcaaaatg gcctcgtagc actgccagac ccgcatttcc atgaacatgg ttcttggggg
    12301 gacggcatag atgtcggaat acacgattgc tccggggccg atcccgtcgc tgaattcgga
    12361 atccatatag gtgaagacca gcttcatgtc ggaacttttt acattgccga tgcccttttc
    12421 cttcagggcg tcatccatgg aggtcagcgt ttcgatgccg agtggaacga atacgctgtc
    12481 ccagtccttc agaagcatgg atttgtcctt tgacccgccg cagccggcca gcagcgctgt
    12541 cacgacaaac agccacacac ggttcatttt catctccggg cgcgggcaag cagagtttga
    12601 gccgagatcc atgcgatgcc gaagcagatc acgacgcccg caaggcccgc aaggcctgtt
    12661 gaaagcattt caaacgatat gaccgggatg gtgtagtccg gcatgaccgc cacgaaggcc
    12721 ggggcgctct catgaaggaa gctgtacttc tccgccaccc attccagccc gtcgggcaga
    12781 gaggacgcaa acggactgat catcagtccg aggactgccg ccgcggagag cgggatggca
    12841 acgctgccgc ggactttctc ttctgcgggc cgggaggcca gcgcataaac cgcggcggca
    12901 gtgatcaggc cttcaccgat gccgatcacc gcgtgggtcg aaatcatggc cgcaatgacc
    12961 gtggagaagg ggatggttcc ggcaacgctg agttcaacgg aaaccgcaaa agaggccgcc
    13021 acaacggata tccatgcggc aatgcccgcg gaagcagccg tgaagattct ggtattattc
    13081 tttgcgctca gcgtgctgtg aatcagtccg cccagccccg tgccgacgat agacatgttg
    13141 aaaatgtttg ctccgagcac cgtgagcccg ccatcggaga agacaagggc ctgcacggcc
    13201 accacgaggg cgatgctcag catgcccagc ggggtgccca gcagcgagga ggccagcacg
    13261 ccgcccagca gatggccgga tgtgcccccg gagatgggga agttgatcat ctgcgccgcg
    13321 aagatcagcg cggtaaccgc agcgaaacgt ccggcgaccg gtttgaactt tgacttaaca
    13381 gcggcaaagg ctgctccggc gatcccggcc gcgctggcgg cggccgtcac tggacaaatc
    13441 gttccctgca acatggcttg cggtatatgc atattctgct cctggttaac ttttcgataa
    13501 atattgactg aatgcgcggg aatatcaagt ccgcttgtgc aatctgcaat cattctgtgg
    13561 aaacattttc ttcccgcgac cggcgcagtg cttcaaggat ctttggatcc tttgtataat
    13621 cctcggctgt tttcccgctg ttgtctttaa gtgtcgcgtc cgcgccccga gaaagaagcc
    13681 attgaacatt atctgttagg cctttctgga ctgcagccat aagcgcggtt tctcccagat
    13741 cgcttgtgct gttgacatca acaccccgat ccagaatgct ctgcatgagt tcaccattat
    13801 cccatgccgc gctgcgttcc tgcgccgcgc gcagcagcgt gtcggccgtc acatctgcgc
    13861 cggatttgat caacagcact gcaatgtgat cgtgttccca ttccaaggct gcgtccaggg
    13921 cggtcatgcc ggatttcccc ttgaagttta catctgcccc gtgtttgaga agcatttgga
    13981 tgacatttgg atcacctccc cttgcagcat aaatcaatgg cgtcaggttg agccggtcaa
    14041 cggcattgac gtttgctcct ttctcaatca gaagcagtgc cccgtcgtga cagcggtctg
    14101 tgatcgcaga catgagaggc gttttgccat acgcgcccgg atcatccacc ggtgccccgt
    14161 gctccagaag ccatgccatg gagtctgtcc ggcagcaggt tgcggccgca tgaagggcgg
    14221 cttcccattc ctgattattc ctcactgaag tcgccgccaa acgtcgtgca ttgacatccg
    14281 ctcctcggga aaccagtact tccatcattt ccagattccc ctggcaggct gggcccatca
    14341 gcggagtggg gccgggatcc gcccctcgat ccagcagaag ttccaccgtg tcctttttcc
    14401 ccgtgaatat ggcccattgc acaaccgtca tgttatccag ccccaccgtc acgttcatgt
    14461 cggcgccgtt atcgagcagc atgcgcacgg cttcattgtg tccattctgc gcggccagaa
    14521 tgagaggcgt ccgcccgcct atgcgctttt cgttgacatc agcccccgct tcaagaagtc
    14581 gtgcgatcag gttcgtgcag tccgtcatgg ccgccatcat gagaggagtg gggcaattct
    14641 gtttctccgg attctgagtg actccgcctt tatccagcat caggtgtgcg gcctcagtat
    14701 gaccggcccg tgccgctctg ccgaacggac tttcactgta tttgctgcag gcgtgaggat
    14761 cagcgcccgc atccatcagt tcttcaatct tcgcgagttc ccctttttga gcggcggcca
    14821 tgagttcagc ctgcagatcc tgtgtttgtg ccgttgcgca gcaagccagt gtgagaaaac
    14881 atgcgagtgc tgcatgcagt ttatgcatta atcggtattt tcgtgacatc gaagtcctcc
    14941 agccaaatca atgatatccc cctaaaaggc ttcaaacatg gttgattcag aagtcacctt
    15001 atctgaatgt gtgtgcacat aggaatacac gtgcagccag acaacgaaga cggccagaat
    15061 ataggcgatc cacttcatca gtctatggtc aagtctccgt agataatctg tttgcaaatc
    15121 gtcatgggaa gcaggacggt gtccatgacc agtgaaagcg ggaattcaaa tacatagtag
    15181 aaggggaata tgcacacttc aatggcccgg cccgggtcat ccattcctgt ggtcccgttg
    15241 atgacggctt tcgcatgaag aatgttgcac gcagttccgc tgtagatctg tttgaccttt
    15301 accggcgggc cgcattcata catatcgtct ttatccgttc caaactgtgt gatcagggag
    15361 gaacagccgg taaacagtga cagcaggata aaacacagcg cttttttcat aataactaaa
    15421 cattgaatct gatttcgatg acgtcgccgt ccttgacgac ataatccttg ccgttcagga
    15481 catagtaatt cttctctttg gcggtgtgtt cggagccggc ctcgatgagg tgatcatagg
    15541 agatggtcac ggcccgaatg aatccgcgct caagatcggt gtgaattttc ccggccgcct
    15601 gcggggctct tgtgcccgcg cggagcggcc acgccttgac ttcatccggt ccgacggtga
    15661 agaaactgat caggcccaga cttgcgtaca ccgcgcgcga caggcggtga gcggccggct
    15721 cttcaatccc aagatcagcc atgaacatcg cgcgttcttc ttctgaaagc tcaagcattt
    15781 ccttctcaat ggatgcgctc agatacacgg gctccgcacc atgctttttg atccgcgttt
    15841 cggtgtcctt caatgcttcg cgcgctttgg aaatgttttc accggtattg aagacggtga
    15901 tgacgggctt cagagttaga aatttcaggc tggcaacaag tttaagttcc tcatcggaga
    15961 actgcatggt cagcagcggc gcgccggttt cgaggtgctg ctggcaccgt ttcagaacgg
    16021 cctcctcgcg gtcgctggtg gtgtcgccct tcttcttgat ttccttcgcc atgcgggtca
    16081 ggcgcgtctc gacggttccc agatcggcca gcagcagttc ggtctcgacc agatcataat
    16141 cgcgcgccgg gttcacatcc cctttgatat ggaaaacctc cggggcttcg aaggcgcgga
    16201 cgacatggag cagggcatcc acgcgccgga gcggctcgat ccagccggca ttgcggctgg
    16261 catcgggctg gatgtcgggg ggcagggcaa tctcgaactc cgcatagcgc gtgcgcttgg
    16321 gcttgtacat ggccgaaagt ttatccacgc gcgggtcgcg cacggctgcc atggcatagg
    16381 cgatgccgtc cttcgtgaca gccttctgcg cgtggatgcc ggtcagcagc tcaaaaacgg
    16441 tttttttgcc cgtctggggc agtccaatga tgcctagatt catgtattcc ttttccgtaa
    16501 agcaccaaag ctatcgcact tgagtccggg ttacaagtct tcaggaatcc tgaaaggccg
    16561 gctgccggga tggataacca ccagtttctc gtccatcaat tccccctcgg catcgtattc
    16621 tctgacactg ttgagtcgca cgcccgtgtc tgccatgctt aaggattccg tgaccgtcca
    16681 gccgctggcg ccatgaatca cggtgatggt ctgcagtgtg atgtctttat cgatatattc
    16741 ataggttgtc atctggagca cattcccgtc gggatcaaag atgacatgcc ggatcatgcg
    16801 gccctgttcg tcatgggtca caaggggaag tggagatgcg tcgccgcccg gcggcatgat
    16861 atcctgtccg accgcaacgg cgcctgtcag cagagccgtc cataaccacc ataaccgctt
    16921 tatcttcatg aaagcgcctc ccgatatgac gatggtctgc tcattgcgac ccgcgtcaca
    16981 tcttgcacgc tctctgtccg agcactaaat atacatcaac tttccgaata atgcgcggag
    17041 cgcagaaatc ccggatttat cgtgagaagc ccgccggggg cgcgttcaga aatggacggg
    17101 acagagccta tccctacacc cccgccgcga aacaaggctt cacgggctat tgttgattct
    17161 gacggaacgg ctgccgggat tgacgagagc attccggtcg ctgagcaggt tgccgccggc
    17221 atcgtactgt ttgacgtttt tgagcccgac ggcgctgtcc gtcatgttgt aggtttcagt
    17281 cacggtccag ccgttggtgc tgtcatagac agtgacggtc tcaagcgtca ccccgccgtc
    17341 aggatactga tacgctttaa cggtgttcac gctgtggtcg ggattatact gcacctgacg
    17401 cagaatcctt ccctggccgt cataggtgat cagcggcggc gggagtatgc tccggggacc
    17461 ccgtgtggcg caaccggagg ccagcatgat catcagaccg ccagcaaggg ctgtaaattt
    17521 catcagtcca agactaggtg atgccatata tttaaacaac attattgtca cggtgttcca
    17581 ttccgttcat gatgcgccgg taaaaaagga atcaactatg gaagcgctga tcagagacat
    17641 caagcagttc gcgcaggagc gcgactggga acagtatcac tcgccccgta atctcatcat
    17701 ggccctttcg gtggaggtgg ccgaagtgat ggagcatttt ctgtggctga cgggggagca
    17761 gagccgcaat ctgcctgaaa agaaaaaaaa cgtgatcatg gacgagatcg gcgatgtgca
    17821 gatccttctg gcccacctgg ccgatcagat gggcattgat ccgattgaag ccgcccgcca
    17881 gaagctggta aagaaccgcg caaagtaccc ggcggacaaa gtgcggggca aggcgctgaa
    17941 atacacggaa tacctgagcg tggaataatg gatttcgtcg cattcgacac cgaaacaacc
    18001 gggatatctc cgcaccgcga tgccgtcatt gaaataggcg cggtgcggtt cagcgacggc
    18061 atcccggggg aatctttcag cgagctgatt gatccgggcc ggcccattcc tcgaggggcg
    18121 accagaatac acgggatcac ggacgagatg gtgagcagca gtccgcagat tggagaagtg
    18181 ctgccgcgtt tcgcggagtt ctgcggagat ctcccgctgg tggcgcacaa tgcacgattc
    18241 gacttcaaat tcatcaacgc ggccgtgcgg agactggcct gcgcggctcc gacgggcctg
    18301 gtgctggaca ctctgacgct gagccggaag ctgtggcccg gacttccaaa ctatcagctt
    18361 ggcacgctgg tgcgcgtgtt tcaggtgccc tgcggccggt tccaccgggc cgcggaggat
    18421 gcgctttact gcgggcatgt ctttcagatt cttctggagc ggtgccggat caactggccc
    18481 gatatggatg cggatggcct tgtaaaaatc tgcggcaggc gtaagatgcg gttcatgcag
    18541 tctgcggagg accgggccgc cacatggttt gcggactttt ttaactgaga aatgaggatg
    18601 cgcatgacat ataaaaaagc aaatgcagcc ctaaccgcgg tatgcgcaat tcctctggca
    18661 gccatgcttc tggccggatg cgcagggctg ggcaccgaaa gtgccggtta cattcatcac
    18721 cgcatggaga gcggaaccac gccgccggcc ggcgaagcgg tttctgtgcg gggcattgtc
    18781 catcaagtcc tggtgttcag ctcaaagagc acgccctcct attacggttt tgtggtccag
    18841 aatccggccg atgcggctga ccgcgatccg tccacgtccg acggtcttta tgtggatatg
    18901 catacaaacg gatttctgcg cacctcgtcc ggcggcattt atcgtccgca tgtcggcgat
    18961 gacgtcctcc tgaaaggggt gatgggaacc agcgcgggcc agaccgtgct gcagcaggcc
    19021 gagttcagga agctgatgcg gagcaggctc aacattgatt cagaactgaa ggcttttgtg
    19081 gccaatcccc cggatgacgc cggcgaggcg gaaatctact ggcgcaggca tgaaggcatg
    19141 cgctgcaaag ttcccgcggg cgcggtggtg tccggcgcgc accgcgacag catgggatcc
    19201 atggtttatc tgatccatcc ttcgcatccg ctgctccgcc gtgaaaacaa ctatgcccgc
    19261 cgcgcgttcc gcgatccgca cccgctggac gaccgggagg aaatcttcga caaccagaac
    19321 ggctatctca tcgggctcgg gccgctgggt ctcgatcgtg ccgacggcga ccccatgccg
    19381 ctgtttcccg aaacctgcac ctttgaagtt cttaaagagc cggtgatggg cgcggtggcg
    19441 cgcttcatgg gccagtacat ggtgcaggtg gacagcctgc ctgtgtttga ggtcggcaca
    19501 gatccttccg ccaaccggtc ggatgctgat gcgggagatg aaagtgtgct gcggatcgcg
    19561 actttcaatg ttgaaaatct ctatgaccac cgtgacgatc cctttgatga ccgcgactat
    19621 tttgaccgaa aggatgcgct gcccattctg gatgcggacg gtaaattcca gggctactcc
    19681 ggcctcaaac tgtatgacta tgtttccgcc tccaaagatg cctacaaggc caggctgagc
    19741 ggactggccg gtcaaatcat tgaagatctt caggcgcccg acattctgat gctgcaggag
    19801 gtggaagatc aggatatcgg cgcggtggag gaggggaaac ttgttgtcgg ggacatcaac
    19861 aaccgcgacg ggaaactcga cgtgctgcag gaactggcgc tggaaatcag caggctgggc
    19921 ggaccggcct atgaacccgt cagcgatcgc gccgccgccg acgaccgggg aatcatcacg
    19981 gccttccttt atcggcccga tcgggtcgcg ctcgcggaga ttagcgaaga tcatcccgtg
    20041 atgggcgtga tgccgtgtgt gccgtctgcg gggcggacca tgacctactg cggtcgggct
    20101 ggaaatccca aggcattcaa tcttctgcat gacattctgg gtgtggtcca tgagcgcgga
    20161 ccgcagatgg ccgcattcga tttcggcgcc ggggagcagg cccgccgcat ttatctgctg
    20221 aacaatcact tcaagagcca gccgcagaac tatgtgcccg agcgccagaa tcaggcgcaa
    20281 ttcagcgccg atctggttcg cgccctgcgc aaagatgaca aaaaggttct ggctgcagtg
    20341 ggcggtgact tgaacacatt cccccggccg gacgagcctg atccgatgct gccgacggat
    20401 cagctgggcg ggctctacgc ggccgatatg gtcaacctgt atgacgaaat gctgaaaacc
    20461 aggccggaga atgcctacag ctatgtcttc aagggacagg cccagacgct ggatcatctc
    20521 ttcgtgacag aaagcctgca caaaaagctg aagcacgtga acgccgtgca catcaacagc
    20581 gactttgccg gcagcgaaaa atatccccgg cgcggacaaa gcgaccatga tccgatcctg
    20641 gccggtttcc agttctgaaa accgggccgg aaacagggag cccgccgcct aggcaagaat
    20701 ccagtgaaca gcccgggcct gcgcccggat cgttccgtcg ccgcttttca tttcggcttc
    20761 gaagaacagc tgccggtcat tctgtccggt gaaccgggcg gtgacggtca ccgcgcccag
    20821 atccggggtc acaggccggc ggaatttcac ttccaggtta cgcgtgacgt tggccggggc
    20881 aaccagcagg ctgagcggtc cgatcgtgtc atccagcagt gtcgcaatga ttccgccctg
    20941 aagacttcca taggggttca gatattccct caggacggga aaccggttgg ttagtatacc
    21001 tgcgccgaga tcgaaatcca gcatttcacc cttcatggcc gtataaaccg gcggcggata
    21061 aaccaaatcg gcggattttt cgcccagttt tgccttcagc gcctccaaat atctgcttct
    21121 gatgccgtcc gactccattt caagcccttt ttagttgatt aggcccgcat aatgctcaat
    21181 attgggggcc gtttaaagcg gaatcccatg aaaatcgtaa taattggagc cggcaatgcc
    21241 ggaacacagc ttgcggaacg tctctgcgaa gagcgtcaca gcgtggtgat gattgatgaa
    21301 cgcatggacg tcctgatgca ggcggaagct tcgctggacg tgctgaccct ttgcggttcc
    21361 ggctcagatc cggtgctgct ggaaagagcg ggcaccgaca aggccgatct tctgatcgct
    21421 gtaaccgacc gggatgaaat caacattata gcctgcctgc tggctcattc cgtcggagtg
    21481 ccgcgtgtgg tggcgcgggt aggcagcgcc cagtacctgc gctcgtcatc caaatacaat
    21541 ctcaagaaca tgggcatcaa cctcgtgatc aaccagaaac aggcctgcgc cgaggaaatc
    21601 tgcagcgcgc tggctctgcc cggagccatc gaatccttcg acctgtttga gggccgcgtg
    21661 atggtggctg ggttcaatat ttcggccgac agcccgattc ttggtctgac accggccaca
    21721 ctgccggatc aggcgctggt ccgcaagctg cggatcatgg ctgtccgccg gaaggagaaa
    21781 ctgattattc cgcgcgggga cacatctttt gctccgcagg attccattta tctggttgga
    21841 agtccggatg acatacgcac tttctcgatc tggatgaacc ccagccagac gccgttcatg
    21901 aaagtgattg tggcaggcgg cggggatatc ggactcacca ttgcccagac tctggaagaa
    21961 cattcggatg ccgaaatcgt gatgctggag caggatgagg accgggcgct gttctgttcc
    22021 ggcgaactca agcacacgct ggtcctgcgc gccgacgcgc tttccggatc cgcgctcgag
    22081 gaaacgggca tcgtgccgcg cactgcattt gtcggtatga ccggcgacga cgagaacaac
    22141 gtcatgaact gtcttatggc ccgcaaaaaa ggcgcggact acaccattac gcagattgcc
    22201 cgcacggatt acattccggt ggtggaaagc ctcgggctgg tggaccggat catcagtccg
    22261 tttgtatcca ccacgcgagg catcctgcac tatctccgaa gccggaacgt ccgggcggcc
    22321 acactcctgc acaaccttcc cggcgaactt cttgacatca tcgtcatgcc gggaaacaaa
    22381 tacatcgggc aggccgtgca ggacatcaaa ttcccgcgcg aggcagtgct ggcggttgcg
    22441 ctgcggggga atgaaattct cccggccacc ggggatctgt ccatcatggc ggatgaccgc
    22501 ctgctggtgt tcgcggattc ctccgccgtg aaaaagatcc agaccatgtt tttcaagtga
    22561 cacagccctt atgaacaaac gcgctgtatt ccatctggtg gcctatatga cccttgttat
    22621 cggtctcgcc atcacggcct gcgcggccgt ttcctggtac tatggcgaca ccttcacggt
    22681 gatcaatgcc ttcatccact ccggggttat caccattgcg gtgtcgggcc ttgtcggggt
    22741 gctcacacac ggcgacatca atctttcccg gcgggacggt ttcggggtgg tgaccatcgg
    22801 ctggctttcc gcggcgctgt tcggcgcgct gccctatgtt ttttccgggg tgatccttca
    22861 tccggtatcc gccgtgtttg agaccatctc gggcttcacc accacagggg cttccgtgct
    22921 ttcggatctt gaagcgcttc cgcgaggcat tctcttctgg cgcgcgctga cgcactggtt
    22981 tggaggcatg ggcgtgctgg tgctgtgcgt cgcgatcctg cctttcctcg gtgtcggagg
    23041 catgcagctt tatcgcgccg aaatgcccgg cccgtccaag gaccgtctga ctccgcgcat
    23101 caccaccaca gccaagcttc tctggggcgt ctatgcgctg ctgactgttt ttgaggccct
    23161 cctgctcaaa tacatcggca aaatggactg gttcgactcg ctctgccaca cctttgcgac
    23221 catggccacg ggcggattct ccacccgcac cgccagcgtg ggcgcctata acagtgcggc
    23281 tgtggacttg atcatcctgt tctttatgtt catggccggc atcaactttt cccttcacta
    23341 ttacgcgctc accggccgcc ccggccgtta tttcagggat ccggaatgcc gcgcctattt
    23401 tcttctcctt gcgggatgcg ggctgttcat cactttcaac atctgggccg gcggctatgc
    23461 tgcgtttacc cgctgcctcc gggatgcgtt tttcaccagc gcctcaattc tgaccacaac
    23521 cgggttctgc acagccgact tcgaccgatg gccggatgcg tcgcgcctgc tgctggtgct
    23581 gatgatgttc gtcggaggct gcgccggttc caccggcgga ggaataaaaa tcgtccgcgt
    23641 aatgatcatg gtcaagaaaa tcatccgcga aacaaaactc ttcatgcgcc cgtccgccgt
    23701 ccttcagctc aaactgggaa agaattcagt ggaccaggag atcatctcgc acatttccgc
    23761 attttttacg attttcattc tgatttttgg gctttgttct tttctgatgt cttttttcac
    23821 tccgaatctc gaaaccgcca ccacggccgt gatcgccgca ctcgggaaca tcggtccggg
    23881 ccttaacgcc gtgggcgcct cacagaacta tgcggccatt cccgccgccg ggcaggggat
    23941 actgatcgtc tgcatgttgc tcggccgcct cgaactctac acggtcctga tccttttctt
    24001 ccccggattc tggaagcgct gaagcagcgg tttcgacaga acgaaaccct ccatcattat
    24061 cgccggaatt gaaactgcga taagcacaaa aatcgcgaaa gggggaaaga tggaatggag
    24121 ggacgcgctc cgtcgcgtcc gtctttggat tttgcccttg gatggaaatg cagaggccgc
    24181 atcacatcat cacaccaggc catccacttt gcagatgcat tcctgaaccg gttgtgctat
    24241 ttcttgtgcg tttcaacagc gggagatttt accatgcagg attttgagaa actcggcgcg
    24301 ttttatctgg ggaacattta cgacagagct gcggggaaac gaacggaaga tattctgctc
    24361 tacgactcca aggatctcac cacacacgcc gtctgcgtcg gcatgaccgg cagcggaaaa
    24421 accgggctct gcatcggact tctggaggag gccgctattg acggcatccc cgccattgtg
    24481 attgatccca agggcgatat cgggaatctg ctgctgacgt tcccgaatct tcagcccgcc
    24541 gatttcctgc cgtgggtgga tgcggccgaa gccgcccgga aaaacatgac ccccgaacag
    24601 ttcgcggaaa aaactgccga tacatggaag aacggcctgg cctcgtgggg acaggatgcc
    24661 tcacgcattg cgatgctcaa ggacaaggcc gacatggcca tctacacccc cggaagcgat
    24721 gccgggcttc ctctttcgat cgtaaagtct ttcgccgcgc cgccggaatc gctgcgcggc
    24781 gaccaggatg ccatgcgcga ccgcatcatg gccgccgtct ctggcctgct ggccctgctg
    24841 ggcgtggatg ccgatcccat ccagagccgg gagcatatcc tgctgtcgaa catcttcagc
    24901 gccgtctggc aggagggccg cgatctcggc ctgccggacc tgatccgcgc catccaggat
    24961 ccgcccttca aatccgtcgg ggtttttgac ctggagagtt tttattcgtc gaaagaccgc
    25021 atgacgctgg ccatgcgcct gaacaacatg ctggcttcgc cgggcttcag cgcctggatg
    25081 aacggcgagc cgatggacat ccagcgcctg ctctatgctc cggccggcaa gccgcgcatt
    25141 tcgattcttt ccatcgcgca cctgaacgac tccgagcgca tgttcttcgt gaccatgctc
    25201 ctcaacgagg tcatttcctg gatgcgcgcc cagccgggca cctccagcct gcgggccatt
    25261 ctctacatgg atgaaatctt cggctacttc ccgccgacgg ccaatccgcc gtccaaaacg
    25321 ccgatgctga cgctcctgaa gcaggcgcgc gcctacgggc tgggatgcgt gctggccacg
    25381 cagaatccgg tggatctgga ctacaagggc ctctccaaca ccggcacctg gtttatcggc
    25441 cgcctgcaga ccgagcgcga caagatgcgc gtgctggacg ggctggaagg cgcttcggga
    25501 agcgccggaa aatccttcaa ccgcgcggag atggagcaga tcctcgcggg tctcggacag
    25561 cgcgtgttcc tgatgaacaa cgtgcatgat gacgcccccg tgctcttcga aacgcgctgg
    25621 gtcatgtcct acctgcgcgg ccccatgacg cgcgcacaga ttcagcagct catggatcag
    25681 cgcaaaagtg cggcggaaca gccggcttcc gtcatggcgt cagcaggccc tgtcgcgacg
    25741 gcctccaccc ggcccgtggt ggctccggga atcacggaac tgtttctcgc cgcctccaaa
    25801 atgcccgtgg acggttcttc cgtggtcctc cgcccggcgc tgctcgccaa tgttaaaatg
    25861 cacttcacaa atgcaaagac caaaacagac gagtggcaga ccagatgcct gatggcggaa
    25921 attccggaaa acgcgggcat gatggactgg gaggcgtcgg acattcttgc atccgagccg
    25981 cagttctcaa gcggcgcggt tgcgggagcg aaatacgcag atctgcccgc atctgccggg
    26041 caggcggcca gctacaagtc gtgggaaaag accctgaaaa gctatgttta cgaaagcatc
    26101 gtgctgaatc tgttcacatg tcccgcgctg aaagaagtgt cgaaaccgga agaggaggag
    26161 ggggacttcc gcggacgtct tgcacagtcg gcccgcgagc atcgcgacgc cgcgctggaa
    26221 aaggtcaaca agacctatgg aagcaaaatg accaccctgc agaaccgcat ccgcgcggcc
    26281 gaggcgaaga tcgagcgcga gaaggcccag tacaaggacc agaagctcaa gaccttctta
    26341 tccatgggcg ctaccgtcct cggcgccatg ctcggccgca aggcggcatc cgtcagcacc
    26401 gtcgggcgcg ccgcgaccac catgcggagc gcgggaaaaa tctcctcgca gaagggcgat
    26461 gtggcccacg cggaaacgaa tctggaggcg ctgaaccagc agtacgcggc gctggatgca
    26521 gaatatcagg ccgagctgga aaaaatcatc atcgaaaaat tacaaactat ttacgatccc
    26581 gaaatcccgg taaatattta cgatctgggg tttatttatg aaatccgaat cgatgacaat
    26641 taccaggtaa cgataaaaat gacacttacc tcgcccaatt gcccggtagc agaaacttta
    26701 cccattgatg tccgcgatgg catccaggct atttccggga tcaaaagtgt tta
//
LOCUS       DAOQWR010000018        25932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2194_length_25932_cov_6.051822, whole genome shotgun sequence.
ACCESSION   DAOQWR010000018 DAOQWR010000000
VERSION     DAOQWR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25932
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_25932_cov_6.051822"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1169)
                     /locus_tag="PKK36_02760"
     CDS             complement(57..1169)
                     /locus_tag="PKK36_02760"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNR93510.1"
                     /translation="MRVLVSMDSFKGTLSAPEACECVRRGLLKARPDLEVTACPMADG
                     GEGTLDVLLGRPGGVRMEREVAGPFPGRRVRAAFGWFPESQTAVVEMAQASGLLLVPG
                     ADRNPMKTTTFGTGELLLAALELQPREILLTVGGSATVDGGTGAALALGWRFLDEKGN
                     PAGFGGEALSKIRKIEPPPAGFSPRFRVLCDVDSPLCGPDGAARVYGPQKGATPVMVA
                     ELERGLENLARAVKEQVGKDITAIPGGGAAGGLAAGAAAFMNADLVPGSQAVIEAIGL
                     KSEIRRADYVITGEGRFDGQSARGKVVAGVAATAKECGVPVIVMAGDVDASASKGRME
                     ILRAIAARRPGMPLDAALKRAAVLLEEAAAGAASSL"
     gene            complement(1171..2106)
                     /locus_tag="PKK36_02765"
     CDS             complement(1171..2106)
                     /locus_tag="PKK36_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNR93511.1"
                     /translation="MPEILNGLSSWFEINMGLSADTQLKILWTLAVLSILFLTHYVIC
                     FLFSVRVKDAARRCTLRKTMTMALSTAGLVFFLRIWIAGRHGLAAYLGILSAGLAIAM
                     QDPLKNIAGWLFIASRKPFDVGDRIQIGDHKGDVIDRRIFQFSLIEIGNWVCADQSTG
                     RIIHVPNGMVFSQAIANYNQGFNFIWNEIGVLVTFESDWKKAKEILMKIAEAHTAVQS
                     AHAEAQVRAAARKFLIFFQHLTPIVWTNVEDCGVKLTMRYLCEPRKRRSSQAAIWEAV
                     LEAFSRESGIDFAYPTIRRYDDRAEGKVQQARAER"
     gene            2290..2820
                     /locus_tag="PKK36_02770"
     CDS             2290..2820
                     /locus_tag="PKK36_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93512.1"
                     /translation="MKRFFAILIALAAGGCGSMPERYIPSATGPIGAPEVVRGLSVSI
                     AAASDAVAFGQPVFFDITISNASRMVYWLPREPQIIFAWTYANGRRDNLIRDPLEDRY
                     FTQYAASKIFPGEVRKHQTKIPTGYFPAKGITEFKAIYTSPRNTNPELMPFWNGEILS
                     NAYGVDIKGRKYTGRH"
     gene            complement(2823..4538)
                     /locus_tag="PKK36_02775"
     CDS             complement(2823..4538)
                     /locus_tag="PKK36_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNR93513.1"
                     /translation="MKNRKAVPEFPPAWAAEAVWYQIFPERFRNGCASSDPRMEDISG
                     THVPGWNITPWGKDWYSLSPWEAEAGGFQKSVFMRRYGGDLVGVREKLDYLQNLGVNA
                     IYLNPVFMSPSLHKYDGSTYHHIDPTLGPDREGDVRLLSSSSETEDPSTWIWTAADRF
                     FLELVRDVHERGMRIIIDGVFNHTGTEFFAFRDLRDRGPASRFRKWYRRARWDENGRL
                     HYKGWFGHPSLPELGRTGKSLTAPVRDYIFASTRRWMAPNGSVRDGIDGWRLDVAFCV
                     PHGFWREWRALVKSINPSAFLCGEIVKLAEPYLRGDELDSVMNYMWSYPVISFFSPAP
                     HPMTAGSLKRRLNRIFEAYGFEVCLSLQNLLDSHDTGRILTMLENPGRPLKKWDEYFN
                     VARTDARPGLITTKPGEQAREVLRQIVVFQMTFPGAPMIYYGTEAGMWGANDPDNRQP
                     MLWDDIVYEDEAHAHNGPCGPNERRPDKDLFAHYQKAIALRRSHPALQRGEFRWEPRA
                     RGRLLGFVRREGESEILALFNASDRAKHFELEHAAADLWEDGAAVAPGRIRIAPRGWR
                     VLKTD"
     gene            complement(4545..5912)
                     /gene="rseP"
                     /locus_tag="PKK36_02780"
     CDS             complement(4545..5912)
                     /gene="rseP"
                     /locus_tag="PKK36_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNR93514.1"
                     /translation="MLSIIINIFKILILFGVTVFVHELGHYLVARMLGLQIDVFSIGF
                     GPALLKRRHNGIVYKIGAIPFGGYVALPQMDPGGEEKSDRDGRRLPRIAAWKKIPVAF
                     SGAAGNLILAFVLAYIIFHGGQAYAPEKTNIIGYVYTNSPAYEAGLRIGDEITGLRGA
                     DVSRPPMVITSWEQFLLESSMHERVELRVVSGEGEHRTVLCETEKFWGSRMIPGITPF
                     TYCLVLNVRPNSSAREAGIQPRDRIVELNGQPLFSREHLVMLVGQYRDVTVPVVIERG
                     GATMELQVTPKYDEEIGRALIGIEFNTLDVRRPAEQIKSHATMIFRILQKLATPRESK
                     AASDSLGGPIAIFVIFWLSVQGSILVALSFTCFINVNLAVINLLPLPVLDGGHILFAL
                     IEIITRRPLHRRVADTLANIFAVLLITLFVLLSCRDVKRMVLPMFAGDEVPAAETNAP
                     PVPQE"
     gene            complement(5927..6766)
                     /locus_tag="PKK36_02785"
     CDS             complement(5927..6766)
                     /locus_tag="PKK36_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNR93515.1"
                     /translation="MLKNRVLSGLLMGALLISAVVWMPDFHFGILLIFAFLVVAALIE
                     FYRLLDAAKIGNYKFIGSFFGVLLLSATWARTQADIPDVSGIIIFLFIASVFIRQLAI
                     KVTDGGFLPVAGTVLGFFYIAFLLDFMARLLFDWDGREGRLLLIYMIVVVKFTDIGAY
                     FIGCATGRHKLIPHISPAKSWEGCFGGVAVATAVSVIWQLVSRGNLGVVHFSILQAVI
                     MGVLLSVTGIVGDLIESLFKRAAAVKDSSSVIRGMGGLLDVIDSLLPASPVLYIFAHL
                     FLK"
     gene            complement(6772..7488)
                     /locus_tag="PKK36_02790"
     CDS             complement(6772..7488)
                     /locus_tag="PKK36_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNR93516.1"
                     /translation="MSDSSPSIPRHVAIIMDGNGRWAKQRGLPRIRGHEAGAESVRSA
                     VRTCKKLGIKYLTLYAFSTENWIRPRAEIEGLMGLLRYFLGKEEKEFHENRVRLRVMG
                     RMRDLPEKVQSALNRMMEITAHYDEGQLILALSYSGRAELSDAVRNIARRVRQGALEP
                     EKIDEKTISESLYLPDVPDPDLMIRTSGELRLSNFMLWQLSYSELYFAPVFWPDFREK
                     DLIAAVEEYGCRQRRFGDVK"
     gene            complement(7491..8186)
                     /locus_tag="PKK36_02795"
     CDS             complement(7491..8186)
                     /locus_tag="PKK36_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93517.1"
                     /translation="MKLSIVIPVYNEVKTLAEVLQVVKGVDIPLEKEIILVDDCSTDG
                     TRDLLEKMQSEDSRLKVAFHEVNQGKGAALHTGFAQATGDFVIIQDADLEYDPGEYMH
                     LLDPLIRGRADVVFGSRFQGGGPHRVVYYWHYLGNRFLTTLSNMMTNINLTDMEVCYK
                     VFRREIIQSLNLREKRFGFEVEVTAKVARGNWRIYEVPISYYGRTYSEGKKITWRDGF
                     RALWCIVKYRFVD"
     gene            complement(8183..9064)
                     /locus_tag="PKK36_02800"
     CDS             complement(8183..9064)
                     /locus_tag="PKK36_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93518.1"
                     /translation="MRKKMLAALSVLLILIALVWPVPHSVYNRLTLALSDYMHFPLAW
                     LLFWLVWRMGASRILSAIFVLTLSAGVESLQPLWGRSASAVDLIYAALGVTAGLLLTR
                     KPVKKTLLFLLCAATLLPVCSILWDQCAARRAFPLLASFESPFERGRWTLNGVSVKRV
                     PLHSAHGRYSAEVRVSEAGFQYPGLFLESMPRDWRGAEALLFDIFWPEISAREICVRM
                     DDQKGNPAYANRFQKSFVLQPGMNRIRLECEEFSRTSGGAMMDLSHVMRMGLFYLKAE
                     KDDVFYLDNVRLLKGET"
     gene            complement(9061..11157)
                     /gene="recG"
                     /locus_tag="PKK36_02805"
     CDS             complement(9061..11157)
                     /gene="recG"
                     /locus_tag="PKK36_02805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNR93519.1"
                     /translation="MNDDPVSLGSLADVPVSAIRGVGPARAALFERLGISTLQDLLLA
                     LPYRYEDRRVFARISDMKPDVPASVCGEIRVCGWSPSHRGQGFFEAVLDDGSGLLHCR
                     WFGAPYLKNEIRRGGKLVVYGKISLYRGNRILQHPDFEVLGSGIDDHIHIGRIVPVYR
                     LTEGLGQKTVRRIMWDAVEKYTSALVESLPESILLQWRLPGLSGAIREAHFPTAKEGA
                     EKARFRLVFEEFLCAELVLAARKIHTEKFTAGNPLVAAGRLKSRFTARLPFQCTAAQL
                     RVIDEIEADLRKPHPMHRLLQGDVGSGKTIVAVFAMLTAVESGAQAAVMAPTDVLVTQ
                     HHEVFSEYLVPLGLQVERLSGNMPAVRRRHVLERARKGIIDVLVGTHALIQEAVEFRN
                     LGLVIIDEQHKFGVEQRGLLCGKGTRPDVLVMTATPIPRTLAMTVYGDLDVSVIDELP
                     GGRQKIVTKVITADQLPLAYEFIKKQIASGRQAYVVFPLVEENEHSVRPAAVQMFEKL
                     STSTFAEQRTGLVHGQMNTDDKRAVMDRFHAGEIDVLFATTVVEVGVDVPNANVMLVA
                     GAEQYGLSQLHQLRGRIGRGPYKSYCLLQGDPGTREAWRRLRIMSETTDGFRIAEEDL
                     RIRGMGNLLGPEQSGFPRMRVGDPLADSHILKTARQQAFDIVTSDPMLKDVKYRELYE
                     RARKLYSRVGGFVEVG"
     gene            11445..12137
                     /locus_tag="PKK36_02810"
     CDS             11445..12137
                     /locus_tag="PKK36_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR93520.1"
                     /translation="MVLMPEQFEEKARPVRKNRAQLRAVRTHNKLLNAALMMFSEKGM
                     DATTVEDITERADLGKGTFYRHFSTKEELMSALVQKAVDGLIEEMHKSAAGAGNLKTL
                     LGKLLEAHKNFFNTRREEFILLFQGRVMLKLQRGMEEDFEQPFVLYLGELENMLAPFV
                     PGSLSPIKLKRLACALAGFVSGFLSFAMIGLGANEIDSNMMPMGTIFIEGASAFLSET
                     EKNADADQTAKA"
     gene            12155..13516
                     /locus_tag="PKK36_02815"
     CDS             12155..13516
                     /locus_tag="PKK36_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNR93521.1"
                     /translation="MKDNRLFNETDASTDEPPPAFAAETAESSALQTSLFSSMPVQAD
                     FSRWNNWTWQMQNRIRSIARLRDYFPGLDSTGIHEAGAKFPFAITPYYASLIRRAERT
                     DPVFAMSVPQMAELFDPACLKDDPLEEDEDMAVPGLVHRYQDRALLISTTSCAMYCRH
                     CTRKRVAGRRDTCITRTRLERAVKYLSMHPEIHDVIISGGDPLTMSTEMLETVISAVR
                     SVKSVDIIRIGTRTPVVLPMRITDDLVNMLKKYHPIFINTHFNHPVELTPQAIEACGK
                     LADAGIPLGNQSVLLKGINDDPHVMEELLRGLLKARVKPYYLFQCDLVRGVEHFRTPI
                     SKGIEIMEYLRGRVSGLGIPLYVVDAPHGGGKIPVLPNYIVSTSPTHTVLRNFEGQMV
                     SYPEPGPHKAPHTGKTDRGDSAAGIWELSSGQHTKIQPQNCARLNRRETRKQTAAMLS
                     PEA"
     gene            13552..14538
                     /locus_tag="PKK36_02820"
     CDS             13552..14538
                     /locus_tag="PKK36_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNR93522.1"
                     /translation="MKIGMTYDLRSDYLREGFTEDQVAEFDTDSTIDAIEATLRDLDH
                     DPVRIGHGRALCQKLANGERWDLVFNIAEGVTGRSREAQVPALLELYSIPYTMSDPLV
                     CAVTLDKAITKRLVASAGINTPAYRLIANLRDSQQVELRFPLFAKPVAEGTGKGVDGL
                     SRVDNPEQLHDLCKALLHKYHQPVLVEEYLPGREFTTGILGTGPHARVLGTMEVVIGA
                     HAPSRDYTYEIKERCEEFVVYRPLEHGPLRREAEELALNAYRVLECRDAGRVDIRVAA
                     NGRPAFIEINPLPGLHPHHSDLPMIATQEGMSYRDLIDHIIKSAAARAGLEA"
     gene            14543..16075
                     /locus_tag="PKK36_02825"
     CDS             14543..16075
                     /locus_tag="PKK36_02825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR93523.1"
                     /translation="MKTVLILYNQPRESAAAGVTRESDAGVLAEVAAVAGALKRDGVP
                     FNTAAISCLSELPKVLNTFSDEIVFNLVEGLEGPVGDVNAVPVVCEAFGRGCTGNTTE
                     CLHQSLDKWVTKLLLHEKGLPVPAAWIVPVGQTFSRPPDIMKPLLLKPLLSDASEGIT
                     QSSVVPHPYADIQDRIAELHARFGQPVLVETFIDGRELNVSVIDMGRGPQVMPIAEID
                     FSAFPANRHKIVDYEAKWIENSFAYNNTPRKIPAALPEKTANTIRDLALGAWRAMSCR
                     DYIRVDFRLDANLNPFILEINANPDISPEAGLAAAVAAAGIAYNEFVMQMIRNASGRR
                     IAEKSSGIRPGPAKIDGGVEIRRTMSADVKPLMNILAGTAFFHDNELQIAEEVLLDSV
                     NEFKASDYQSFTACIEGHPAGWVCWGATPCTDRTFDVYWLAVDKKIQGKGIGKKLMTF
                     TEESIWKAGGRLAVLETAGREDYEPTRRFYLSIGYTEAARVKDFYALGDDKVIYTKPV
                     PR"
     gene            complement(16072..17154)
                     /locus_tag="PKK36_02830"
     CDS             complement(16072..17154)
                     /locus_tag="PKK36_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93524.1"
                     /translation="MSRFKKNRGNSPAGASGAGAEITMIEAHRRTRLTSMAFLALMVV
                     FLLLAWWMWAESRKHGDSDKDFLLLSLDTNAAMTDRPATSPGPLYTAPREWTEPSVDS
                     GDAAPPLDPQKMTAAMGEMRKAQEYLQGGNYDAATLHVRKALDIQPDMDAALRMLGVI
                     YIQRGQFDQAISILEKALARDAFNPETLSNLSTAYLQRGDREKAEELLLTAVQIKPDY
                     HIGYVNLGLLFLASAQYESAAEYFEMAMAGMRDSISLRNNYAVALLRLGRFEEAREHL
                     NWLVTRHPDVPESYFNMAITYVYDNDAASALEWIRRGSEHCSPSALQEYLRDSDFNAI
                     RQHPGFQSLARGIEPDLPRRPVAPRN"
     gene            complement(17167..17730)
                     /locus_tag="PKK36_02835"
     CDS             complement(17167..17730)
                     /locus_tag="PKK36_02835"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNR93525.1"
                     /translation="MILVLDNYDSFTYNLVQYMGEMGADVKVVRNDAMTPDELCALKP
                     EKALISPGPCTPDEAGVSCEIIRRLGPKIPVLGVCLGHQCIGQVYGGRVIRASRLMHG
                     KTSPIRHNGEGVFRGMPQPFEATRYHSLLVERASLPECLKITAETEEGEIMGLQHAEH
                     PVHGVQFHPESILTQSGRLIIQNFLKL"
     gene            complement(17727..19241)
                     /gene="trpE"
                     /locus_tag="PKK36_02840"
     CDS             complement(17727..19241)
                     /gene="trpE"
                     /locus_tag="PKK36_02840"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNR93526.1"
                     /translation="MYTPTREGFLEKAKQGNLIPVWRELLADQDTPLCAYERMRQALR
                     SRSEPAQTFLLESVEGGEHIARYSFLGSSPRAVLSSAGRTTTITSRDGNVETIGNREP
                     LSVLKEHLSKYRPVPDLSLPRFYGGAVGYIGYDAVAQFERVPLCARGGTEWPDMMLAI
                     TDTIVVFDRARHTMKVVANALVEEDPEKAYDDAVAAIDSLCRDLSTHVPHVMLDASGA
                     ADEPEFRSNMTRDEFENAVMTAKEFIRAGDIIQVVLSQRFETGTAGDPLDVYRALRSV
                     NPSPYMFCLENGSRAIVGSSPEVHVRCEDGRVELRPIAGTRPRSADDAEDHRLARELL
                     ADPKERAEHIMLVDLARNDLGRVCEYGSVTVPELMVIERYSHVMHIVSDVTGRLRADR
                     DIYDLMRATFPAGTVSGAPKIRAMEIISELEAGRRGPYAGAVCYFGYSGNLDSCIAIR
                     TVLMDGDKSCVQAGAGIVADSDPAAEYMETCNKARGMLKSLVLSRRFGELRGKS"
     gene            complement(19259..19672)
                     /locus_tag="PKK36_02845"
     CDS             complement(19259..19672)
                     /locus_tag="PKK36_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNR93527.1"
                     /translation="MSRELEEGLELALDFDKLAKASAAAPGIIPVAVQNADTHEVILV
                     AYANELALKKSIETRSAVFWSTSRNELWEKGKTSGETFDLLEVRVNCEQNSLVYIVRP
                     RRGGICHTKNRQGAARNCYYRRLNFVTGKLENLDP"
     gene            complement(19685..20446)
                     /gene="hisF"
                     /locus_tag="PKK36_02850"
     CDS             complement(19685..20446)
                     /gene="hisF"
                     /locus_tag="PKK36_02850"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNR93528.1"
                     /translation="MLAKRIIPCLDVARGRVVKGVNFVSLQDAGDPVECARAYSEQGA
                     DEITFLDIRASHEGRDTMVEVVNRVARQVFVPLTVGGGIRNSEDVRILLQAGADKVSI
                     NTAAVERPALISEAAEQFGSQCVVLAIDAKKNEDGGGWTVYTYGGRRPAGMDVLEWAR
                     EGVRRGAGEILLTSMDRDGTKNGYDLALTRAVSEAVTVPVIASGGAGSLEHLYEALSA
                     GRADAVLAASIFHYGTYTIKQTKDYLRERGIPVRA"
     gene            complement(20671..20997)
                     /locus_tag="PKK36_02855"
     CDS             complement(20671..20997)
                     /locus_tag="PKK36_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93529.1"
                     /translation="MAPASGSKAVSLFVAQNAEVDAVILDMTMPGMNGYEAMLALRKF
                     IPAMPVLKISGDVPENPYPHDGCTLFMMKPLGTEALLVCLRGLLEQRTGVQTQKSAAS
                     RHSAVH"
     gene            complement(21541..22203)
                     /locus_tag="PKK36_02860"
     CDS             complement(21541..22203)
                     /locus_tag="PKK36_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR93530.1"
                     /translation="MNKQPAAPNRLRILIVEDHPILCRGLRDILSEEFPDAVFGEADE
                     SRSALQAFRRQPWDVVLLDINIPGRDGLSLLDDVRRIRPAARVLVVSAYPEEEFAVRA
                     LKLGAAGYLGKNEAPEKLVEAVNKILGGGTYVNAILAEKLVTVLTASSDARLPHETLS
                     ARELQVLRLIAEGKTAKKIAEELSLSEKTIATYRARIAEKTGLSTNVQLTRYAFQHNL
                     TG"
     gene            complement(22200..24593)
                     /locus_tag="PKK36_02865"
     CDS             complement(22200..24593)
                     /locus_tag="PKK36_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93531.1"
                     /translation="MSKESPVESRNRKTVAAREYFSGRGRECLEAAGSAFSDFLFVLD
                     RSGVITDYFVVHPELLYMPPDEFLGRDIRKILPHPASEIIGEAINHALKFGGIKETVY
                     SLPYPEGDRWFELAIADQGQSPADSNKLVFIARDITWRKKAEDDLRASRDRLNRTEAI
                     AHLGSWELDLMSNRLIWSDEVFRIFGQPPQSFAATYEAFLELVHPEDRAALDMAYRNS
                     LCEGGEAYQMEHRVVRADTGAVRWVEEKCFHVRDKEGRVVCSHGMVIDVTERRQAEEA
                     LKESEARHRAIHDNLPNGLIYQIDSGEDGQSRRFTFVSQGVEQLHGVTTDEVMKDSGA
                     IYRQVIDKELVAGLEAKAAESMTPFNVEARIRLASGEKRWRWFASAPRRLPNRHLIWD
                     GIELDITERKQAEEALRRSEAKYRLLYENMEDAFIIADMKGRLLETNPAFERLVQYSK
                     EEVTGLSYKVFTPPEWIETDEMAFRQVAERGYTDIIEKEFIRKDGTRVPVEMRGCLLR
                     DEAGNPAGVWAVIRDNTERKQAEQLLRGAHDELERRVEERTAELRASERALSRSREEL
                     RALLSRLEQAREDERIRIARDIHDDFGQSLTAIKMDLRRIEKLAEKISPALLQDKIQK
                     RASGAISLADSALSMVQKLAAELRPGVLDKLGLCTALQYEARCFEERHGIRCTMAVAS
                     RLSEVPPLVATALFRIFQECLTNIARHAGASRVSVRLGMADRSLKLCVRDNGRGIGEA
                     AVNNPASLGLIGIRERAVALGGEAAFRRGNKNGTVVEVRIPLSRAKGGSRRSRKRGS"
     gene            complement(24973..25644)
                     /locus_tag="PKK36_02870"
     CDS             complement(24973..25644)
                     /locus_tag="PKK36_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR93532.1"
                     /translation="MKNAASGKRRVVIVDDHPLFCEGLGRVIDRQPDIVMCGAAADAA
                     GGLAASRKLKPDVVIADLTLEEGSGLEMIECLHAEQPKLPILALSAHPEDVYAVRAYR
                     AGARGYVMKHEPSARVLEALRRLLNGQMAFSEGVISQVMELGIGKDASLPRSPVELLS
                     NRELEIYRAIGLGRATREIAARLGLSVSTVESYRASIKLKLHLRNAAELAASAARFLA
                     EEAAR"
BASE COUNT         5646 a         8056 c         7055 g         5175 t
ORIGIN      
        1 aaaaccgcgg cagggatcga actgcggccg tacgaaacct gctggatccg gatcgcctag
       61 aggctggagg cggcccccgc ggcggcctct tccagcagaa cagccgctcg cttcagggcc
      121 gcatcaagag gcatgcccgg ccggcgggca gcaatggcgc gcagaatttc catgcggccc
      181 tttgaagcag aggcatccac atcccccgcc atcacaatca ccggaacccc gcattctttc
      241 gcggtcgccg cgactcccgc caccaccttt ccgcgggcgc tctgcccgtc aaagcgccct
      301 tcccccgtga tcacgtaatc tgccctgcgg atttctgatt tcaagccaat cgcctcaatc
      361 accgcctgcg agccgggcac aagatccgca ttcataaaag cggcggcccc cgccgccagc
      421 ccccccgccg cgccgccgcc ggggatggcg gtgatatcct ttcccacctg ctccttcacc
      481 gcgcgcgcga gattttccag tccacgctcc agttccgcca ccatgacggg ggtcgcccct
      541 ttctgaggcc cgtagacccg cgcggcgccg tccggcccgc acaggggact gtccacatca
      601 cacaacacgc gaaaccgggg ggagaaaccg gctggaggcg gctcgatttt tctgatcttc
      661 gagagagcct ctccgccgaa tccggcgggg tttcctttct cgtccagaaa tctccagccg
      721 agcgccaggg cggcgcccgt tccaccgtcc accgttgcgc ttccgcccac cgtgagcaga
      781 atttcccgcg gctgcagttc aagcgcggcg agcagcagtt cgcccgtgcc gaaggttgtg
      841 gttttcatgg gattgcggtc agcgccggga accagcagca ggccgctggc ctgcgccatt
      901 tccacaaccg ccgtttgact ctccgggaac catccgaaag ccgcccggac ccgccggccg
      961 ggaaacgggc ccgcaacctc gcgctccatg cggactccgc cgggccggcc aagaagaaca
     1021 tccagcgttc cttcgccccc gtccgccatg ggacaggccg tcacctccaa atcgggacgg
     1081 gctttcagca agccccgccg gacacactca caggcctccg gcgcggacag tgttcccttg
     1141 aaggaatcca tggaaacaag aacgcgcatg ctagcgctcc gcgcgggcct gttgaacttt
     1201 tccttccgcg cggtcgtcgt accggcggat ggtgggataa gcaaagtcta ttccactctc
     1261 ccgggaaaaa gcttccagca ccgcttccca aattgccgcc tgggacgaac gccgcttgcg
     1321 gggttcacag agatagcgca tggtcagctt aacaccgcag tcctccacat tggtccaaac
     1381 aatgggagta aggtgctgaa aaaaaatcag aaacttgcga gcggcggcac gcacctgagc
     1441 ctccgcatgg gcgctctgga ctgcagtgtg cgcctccgca attttcatca ggatttcttt
     1501 ggccttcttc cagtcgctct caaaggtcac cagaacgccg atttcattcc aaatgaaatt
     1561 gaaaccctga ttgtaattgg caatggcctg gctgaacacc atgccgttgg gcacatgaat
     1621 gatgcggccg gtgctttgat ccgcgcaaac ccagttccct atttcgataa gcgaaaactg
     1681 gaatattcgc ctgtcaatca cgtctccctt gtggtctccg atctgaatgc gatcgcccac
     1741 atcaaaaggt ttgcgactgg cgataaaaag ccagccggca atgttcttca gaggatcctg
     1801 catggcaatg gccagccctg cagagagaat tcccagataa gccgccagac catgacgccc
     1861 cgcgatccat attctcagaa aaaaaaccag accggcggta ctgagcgcca tggtcatcgt
     1921 tttacggaga gtacagcgtc gagcggcatc cttcacgcgg acggagaaaa gaaaacaaat
     1981 gacgtaatga gtcagaaaaa ggatgctcaa tacagccagt gtccacagaa ttttcaactg
     2041 agtgtcggct gaaagaccca tgttaatctc aaaccagctc gacaatccgt tcagaatttc
     2101 cggcatcaca gctcccataa ttctgcagac tacagcagtc gctctgtcca ttcaagttcg
     2161 ttcgccggaa aatggggttt ttccagtcat tggaagtttt tccgcagaaa gttccaatga
     2221 ttggaagttt tttctgttca ttttccaatc attggaagat catgcaaaag aaggaaacag
     2281 ggggtgagca tgaagaggtt ttttgcgata ctgatcgcgc tggcggccgg aggatgcggg
     2341 agcatgccgg aaagatatat tccatctgcg acgggcccca ttggcgcgcc ggaggtcgtg
     2401 cgcggccttt ccgtttccat cgccgcggca tccgacgccg tcgccttcgg gcagccggtg
     2461 ttttttgaca tcaccatctc caatgcgagc aggatggtgt actggcttcc ccgggaaccg
     2521 cagatcatct ttgcatggac ctacgccaac ggaagacggg acaacttgat ccgggatcct
     2581 cttgaggacc gctatttcac ccagtatgcc gcgtcaaaaa ttttcccggg tgaagttcgc
     2641 aaacaccaga cgaaaatccc gaccggatat ttcccggcca agggcatcac cgaatttaag
     2701 gccatttaca cctctccgcg gaacaccaat cctgaactga tgcccttctg gaacggcgaa
     2761 attctctcca acgcatatgg cgtggacatc aagggcagaa agtacaccgg caggcactga
     2821 tctcaatccg ttttcagcac gcgccatccg cgcggcgcaa tccggatgcg gcccggcgcg
     2881 acggcggcgc catcctccca caaatcggcc gcggcatgct ccagctcaaa gtgctttgcg
     2941 cggtccgagg cattgaacag cgcaaggatt tccgattctc cctcgcgccg gacgaatccg
     3001 agcagcctgc cgcgggcgcg cggctcccag cggaattcgc cccgctgcaa ggccggatgc
     3061 gaccggcgca gggcaatggc tttctgatag tgagcaaaca aatccttgtc aggccgccgc
     3121 tcattcgggc cgcaagggcc attgtgcgca tgcgcttcat cctcgtaaac gatgtcatcc
     3181 cacagcatgg gctggcggtt gtcaggatcg ttggcgcccc acataccggc ttcagttccg
     3241 taataaatca tcggcgcgcc agggaaggtc atctggaaca ccacgatctg ccgcagaacc
     3301 tcgcgggctt gttccccggg ctttgtggtg atcaggcccg gccgcgcatc cgtccgcgcg
     3361 acgttgaaat actcgtccca ttttttcagc ggccggccgg gattctccag catggtcaga
     3421 atccgtcccg tgtcatgcga gtcgagaagg ttctggaggc tgaggcagac ctcaaagccg
     3481 tatgcttcaa aaatccggtt cagtcggcgc ttcagtgagc cggccgtcat cggatgcggg
     3541 gccggggaga agaaggaaat gaccggatag ctccacatgt agttcatgac ggaatccagt
     3601 tcatcgccgc ggaggtaggg ctctgcaagc tttacgattt cgccgcagag aaacgccgac
     3661 ggattgatgg acttcacgag ggcgcgccat tcgcgccaga agccgtgagg cacgcagaag
     3721 gccacgtcca gccgccagcc gtcaattccg tcccgcaccg aaccgttcgg cgccatccag
     3781 cggcgggtcg acgcgaagat atagtcgcgc accggcgcag tcaggctctt cccggtgcgg
     3841 cccagttccg gcagactggg atggccaaac cagcccttgt agtgcaggcg gccgttttca
     3901 tcccagcgcg cgcggcggta ccatttgcgg aaacgcgagg ccgggcccct gtcccgcaga
     3961 tcacggaaag caaagaattc cgtcccggtg tggttgaaaa cgccgtcaat aatgatgcgc
     4021 atgccccgct catgaacatc gcgcacaagt tcaagaaaga accggtcggc cgcggtccat
     4081 atccacgtag acggatcttc ggtttcgctg gaagatgaca acagacgaac atccccttcc
     4141 cgatccgggc ccagcgtcgg gtcaatgtgg tgataggtgc tcccgtcata tttgtgaagc
     4201 gaaggcgaca tgaaaacggg attgagataa atcgcattca ctccgagatt ctggagataa
     4261 tccagcttct cgcgcacccc cacaaggtct ccgccatatc tccgcatgaa aacgcttttc
     4321 tggaatccgc ccgcttcagc ctcccacggc gagagactgt accagtcctt tccccatggc
     4381 gtgatgttcc agcccgggac gtgtgtgccc gaaatgtcct ccatgcgagg atcgcttgag
     4441 gcacagccgt tgcggaatct ttcaggaaaa atctgatacc agaccgcctc tgcggcccat
     4501 gcgggaggaa attcgggaac agcctttcgg tttttcatgg cgcactattc ctgcgggacc
     4561 ggcggcgcat tggtttccgc ggccggcacc tcatcacccg caaacatcgg caggaccatt
     4621 ctcttcacat cgcggcagga aagcagcacg aacagtgtga tcagcagcac ggcaaatatg
     4681 ttggccagcg tatccgccac cctgcggtgc aggggccggc gcgtgatgat ctcaatcagc
     4741 gcaaacagaa tgtgcccgcc gtcgagcacc ggcagcggaa gaagattgat caccgcaagg
     4801 ttcacgttga tgaaacaggt gaaggacagc gccaccagga tgcttccctg cacggaaagc
     4861 cagaagatca caaaaatggc aatcggcccg cccagcgaat cggaggcggc cttcgattcc
     4921 cgcggcgtgg ccagcttctg aaggatgcgg aaaatcattg tggcatggga tttgatctgt
     4981 tcggcggggc gtcgcacatc cagcgtgttg aactcgatgc cgatcagcgc ccggccgatt
     5041 tcctcgtcat atttcggcgt cacctgcagt tccatcgtcg cgccgccacg ctcgatcaca
     5101 accggaacgg tcacgtcgcg atactgaccc accagcatga ccagatgctc gcggctgaac
     5161 agcggctggc cgttcagttc cacaatccgg tcgcggggct gaatgcccgc ctcccgggcg
     5221 ctggaattcg gccggacgtt gagcaccaga cagtaggtga acggtgtgat gccgggaatc
     5281 atgcgcgatc cccagaactt ctcggtttcg cacagcaccg tacgatgttc cccctcgccg
     5341 ctcaccaccc gaagctccac gcgctcgtgc atagatgatt caagcaggaa ctgctcccag
     5401 ctggtgatca ccatgggcgg ccggctgacg tctgcgccgc gaagccccgt gatttcatct
     5461 ccaatgcgca ggcccgcctc ataggccggg ctgttggtgt acacataacc gatgatgttg
     5521 gttttttcgg gcgcgtaggc ctggccgcca tgaaaaataa tgtatgccag aacgaaggcc
     5581 agaatcagat tgcccgccgc gccggaaaat gccaccggga ttttcttcca tgcggcaatg
     5641 cgtggaagcc ggcggccgtc gcggtcgctt ttctcttcgc cgccgggatc catttgaggc
     5701 agcgccacat atccgccaaa cgggatggcc ccgattttgt acacaatgcc gttgtggcgg
     5761 cgcttcagga gggcgggccc gaagccgatg gaaaacacgt caatctgcag gcccagcatt
     5821 ctggccacca gatagtggcc gagttcatga acaaaaaccg tgacgccgaa cagaataagg
     5881 attttgaaga tgtttatgat aatgctgagc atgatgtcct gtgtggttat ttgagaaaga
     5941 gatgggcaaa tatatagagc accggagacg ccggaagaag gctgtcaatc acatccagca
     6001 ggccgcccat gcccctgatg acagagctgg aatctttcac cgcggcggcg cgcttgaaca
     6061 gcgactcaat caggtcgccg acgatgcccg tcacggagag aagaacgccc atgatcaccg
     6121 cctgaagaat cgagaaatgg acaactccca ggtttccgcg actgaccagc tgccagatca
     6181 cgctgacggc cgtggcgacg gccacgcctc cgaagcagcc ctcccaggat ttcgcggggg
     6241 aaatatgggg aatgagttta tgccggccgg ttgcgcaacc gataaaatag gccccgatat
     6301 cggtgaactt gaccacaacg atcatgtaga tcagcagcaa gcggccctcc cggccgtccc
     6361 agtcaaacag cagccgcgcc atgaagtcca gcaggaaggc gatgtagaaa aagcccagca
     6421 ccgtgcccgc caccggaaga aatccgccgt ccgtgacctt gatggcaagc tgccggataa
     6481 acacggaggc gatgaaaaga aaaatgatga tcccggagac atcgggaatg tcggcctgtg
     6541 tgcgggccca cgtggcggag agcagaagca ctccgaaaaa tgatccgatg aacttgtaat
     6601 tcccgatttt tgccgcatcg aggagccggt agaactcgat cagggccgcc acaacgagga
     6661 aggcgaaaat gagcaggata ccgaaatgga aatccggcat ccacacgacc gccgaaatca
     6721 gcagcgcccc catcagcaga ccgctcaaga ctctattttt cagcataacc cctacttcac
     6781 atcgccgaag cggcgttggc ggcagccgta ttcctcaacc gccgcaatca aatccttttc
     6841 ccggaaatcc ggccagaaaa ccggtgcgaa atataactcc gagtatgaca attgccaaag
     6901 cataaaattg ctgagccgca gctcgccgct ggtacggatc atcaggtccg gatcaggcac
     6961 atcgggtaga tagagcgatt ccgatatggt cttttcatct attttttccg gctcaagcgc
     7021 cccctgccgc acccgccggg ctatgttccg aacggcatcg gacagttccg cgcggccgct
     7081 gtagctcaag gcaaggatga gctggccttc atcatagtgc gcggtgattt ccatcatgcg
     7141 gttgagcgcg gactgcactt tttccggaag gtcccgcatg cgccccatca cccgcagccg
     7201 aacccggttt tcgtgaaatt ccttttcctc tttgcccaga aaatagcgca gaagccccat
     7261 aaggccctca atttccgcgc ggggacgaat ccagttctcc gtgctgaacg catagagggt
     7321 gagatatttg atccccagtt tcttgcacgt gcgcacggcg gaccgcacgg attccgcacc
     7381 cgcctcatgc cccctgatgc gcggcaggcc gcgctgtttg gcccagcgcc cgttgccatc
     7441 catgatgatg gccacatgcc gcgggatgct gggagaggaa tcgctcatgg tcagtccaca
     7501 aaccggtatt tcacaatgca ccagagggcc cggaaaccat cacgccaggt gatttttttc
     7561 ccctcgctgt acgttcggcc gtaataggaa atcggcactt cgtatatccg ccagtttccg
     7621 cgcgcgacct tggccgtgac ctccacttca aaaccgaagc gcttctcccg gagattcagc
     7681 gactggatga tttcgcgcct gaacaccttg tagcagacct ccatgtccgt caggttaatg
     7741 ttcgtcatca tgttggacag ggtggtcaga aaacgattcc cgagataatg ccagtagtaa
     7801 accacgcgat gcgggccgcc cccctgaaaa cgcgaaccaa agactacgtc cgcgcggccc
     7861 ctgatgagcg gatccagcag atgcatgtat tcgccggggt cgtattccag gtcggcatcc
     7921 tgaatgatca cgaaatcccc cgtggcctga gcgaaccctg tgtgcagcgc cgcacccttc
     7981 ccctgattca cctcgtgaaa ggcgaccttg agccgggaat cctcggactg cattttttcg
     8041 agcaggtcgc gcgtgccgtc ggtggaacag tcgtccacca gaatgatttc cttttcaagc
     8101 ggaatatcca cgcccttcac gacctgcagc acctcggcca gggttttaac ctcattataa
     8161 actgggatga ctatggaaag cttcatgttt cacctttcag cagcctgaca ttatccaaat
     8221 aaaacacgtc gtccttttcc gctttcaaat agaaaagtcc catcctcatc acatgcgaca
     8281 gatccatcat cgcgccgccg gaagtccgac tgaattcttc acactccagg cggattctat
     8341 tcatccccgg ctgcaaaaca aagcttttct gaaagcgatt cgcataggcg ggattgccct
     8401 tctgatcatc catccgcaca cagatttcac gcgcgctgat ctccggccag aagatatcaa
     8461 acagcagcgc ttccgccccg cgccagtcac ggggcatgga ttccagaaaa agtccgggat
     8521 actgaaagcc ggcttcactg acgcgaacct cagccgaata ccgcccgtga gcactgtgca
     8581 ggggaacccg tttcacagag actccgttca gcgtccagcg gccccgctca aaaggactct
     8641 caaaggacgc cagcagcggg aaggcgcgac gggcggcgca ctggtcccag aggattgaac
     8701 acaccggcaa cagggttgcc gcgcacaaaa gaaacaacag cgtcttctta acaggtttgc
     8761 gggtcagcag cagacccgcc gtgaccccca gcgccgcata gatcaaatcc acggcggagg
     8821 ccgagcggcc ccagaggggc tgcaggcttt cgaccccggc cgacaaggtc agcacaaaga
     8881 tggccgaaag gatgcgcgac gcgcccattc tccagactag ccagaagagc agccacgcga
     8941 gcgggaagtg catgtaatcg gaaagcgcca gcgtcagacg gttatagaca gagtgcggca
     9001 ccggccaaac cagcgcaatc aggatcagca gcacagacaa agccgccagc attttttttc
     9061 tcatccaacc tccacaaaac cgcccacccg gctgtacagc ttccgggcac gttcatacag
     9121 ctcgcggtat ttcacatctt tgagcatggg atcggaagtt acaatatcaa atgcctgctg
     9181 gcgggccgtc ttgaggatgt ggctgtcggc cagcggatcg ccgacacgca ttcgcgggaa
     9241 gccgctttgc tcgggcccga gaagattgcc catgccgcgg atacgcagat cctcctcggc
     9301 aatgcgaaaa ccgtcggtgg tttcactcat aatgcgcagc cgccgccatg cttcgcgcgt
     9361 gccgggatcg ccctgaagca ggcagtagga cttgtatggg ccgcggccga tccggccgcg
     9421 gagctgatgc agctgcgaga gcccgtactg ttcggccccc gccacaagca tcacgttggc
     9481 attgggcacg tccacgccca cctccaccac cgtggtggca aacaggacat caatttctcc
     9541 ggcatgaaat ctgtccatga ccgccctttt gtcgtccgtg ttcatctgtc cgtgcaccag
     9601 cccggtgcgc tgctcggcga aagtggaggt gctgagcttt tcgaacatct gaaccgcggc
     9661 cggccggacc gaatgttcat tctcttcaac aagcggaaag accacatagg cctgccggcc
     9721 ggatgcgatc tgcttcttga taaattcata cgcaagggga agctgatcgg cagtaatgac
     9781 ttttgtgaca attttctgac ggccgcccgg aagttcgtct atgacgctga catcaagatc
     9841 accgtacacg gtcatggcca gtgtgcgggg aatgggcgtg gcggtcatca ccagcacatc
     9901 gggccgggtt cccttgccgc acagcaggcc gcgctgttcc acgccgaact tgtgctgttc
     9961 gtcaataatg acgagaccca gattgcggaa ctccaccgcc tcctgaatca aggcatgggt
    10021 tcccaccagc acatcaatga tgcccttgcg ggctctctcc aggacatggc ggcggcgcac
    10081 ggcgggcatg tttccggaca ggcgctcgac ctgcaggcca aggggcacaa gatattccga
    10141 gaacacttca tggtgctgcg tcaccagcac atccgtgggg gccatcacgg cagcctgggc
    10201 cccgctctcc acggccgtca gcatcgcaaa aacggcaaca atggttttcc cggaaccgac
    10261 atctccctgc agcaggcggt gcattgggtg cggcttgcgc agatcggcct cgatttcatc
    10321 aatcacccgg agctgcgcag ccgtgcactg aaacggaagc cgggctgtga agcggctctt
    10381 caggcgtcct gccgcaacca gcggattgcc ggccgtgaat ttttccgtat gaatttttcg
    10441 ggcggccagc accagttccg cgcaaaggaa ctcttcaaac accagccgga agcgggcctt
    10501 ctctgcgccc tctttcgccg tggggaaatg agcttcccgg atcgcgcccg aaagtccggg
    10561 gagccgccat tgcagaagaa tgctttccgg cagactttcc acaagcgcac tggtatattt
    10621 ctccacggca tcccacataa tgcgccggac ggttttctgt cccagcccct cggtcaaccg
    10681 ataaaccggc acaatgcggc cgatatggat atggtcatca atcccgctgc ccaaaacttc
    10741 gaaatcggga tgctgcagaa ttcgattgcc gcgatagagg ctgatcttcc cgtaaacaac
    10801 cagtttgcct ccgcgccgga tttcattctt cagatacggc gcgccaaacc agcggcagtg
    10861 gagcagcccc gagccgtcgt caagcacggc ttcaaagaag ccctggccgc ggtgtgacgg
    10921 gctccatccg caaaccctga tttcaccgca aacggaggct ggcacatcgg gtttcatatc
    10981 agaaatccgt gcaaagactc ggcgatcctc ataacgatat ggaagagcaa gaagtaaatc
    11041 ctgaagcgtt gaaatgccaa gacgctcaaa cagggccgcc cgcgcagggc ccactcctct
    11101 tatggcggaa acgggaacat ccgcaagcga acccaaactg acggggtcat cattcataat
    11161 gctgatcgta ctctataaaa aagcgcgtaa agataaaagc acaggtattg aaaagctgac
    11221 cgggaaagtt cgattattgc gttaatacag ggccaaccgg ccgccaacag ggcgggagat
    11281 tcaagacatc aacaacttgc ggacacgaaa aaaacgccga ttccctcttg actttttttc
    11341 cagatatgac taattggtca ctgttagatg ataaaggttt gtttttgagg cgggttcgca
    11401 agtgactgtt tggtccgtcg ctaaaatacg ctggaagtat aagtatggtg ctgatgccgg
    11461 aacaatttga agaaaaagcg cggccggttc gcaagaacag ggcccagctg cgcgctgtgc
    11521 gcacgcacaa caaactgctg aatgcggctc tgatgatgtt ctccgaaaag ggcatggatg
    11581 ccacgaccgt tgaggatatc accgagcgcg ccgacctcgg gaagggcacg ttctaccgcc
    11641 atttcagcac caaagaagag ctgatgagcg cgctggttca gaaggcggtg gacggcctga
    11701 tcgaagagat gcacaaatct gcggcaggag ccggcaacct taaaacgctg cttggcaaac
    11761 tgctcgaagc tcacaagaat ttcttcaaca cacggcgcga ggagttcatt cttctctttc
    11821 agggccgcgt catgctcaag ctgcagcgcg gaatggaaga ggattttgag cagcctttcg
    11881 tgctctatct gggggagctg gaaaacatgc tcgccccctt tgtgcccggc agcctcagcc
    11941 ccatcaaact caaaaggctc gcatgcgcgc tggccggatt tgtgtcgggt tttctttcct
    12001 ttgccatgat tgggctcgga gcaaacgaaa tcgacagcaa tatgatgccc atggggacca
    12061 tttttatcga aggcgccagc gccttcctca gtgaaacaga aaaaaacgcc gacgcggatc
    12121 aaaccgccaa agcctaaatg gagaaatgca gatcatgaag gacaaccgtt tattcaacga
    12181 aacggatgcc tcaactgacg aacctccgcc cgcgtttgcg gcggagacgg ctgagagctc
    12241 cgcattgcag acatcgctgt tcagcagcat gccggttcag gctgatttca gccgatggaa
    12301 caactggacg tggcagatgc agaaccgcat tcgcagcatc gcgcgcctgc gcgactactt
    12361 cccgggactg gactccaccg gaattcatga agccggcgca aagtttccgt tcgcgatcac
    12421 accctattat gcctccctga tccgccgcgc ggaacggaca gatcccgtct tcgccatgag
    12481 cgtgccgcag atggccgagc tgtttgatcc ggcctgcctg aaagatgatc cgctggaaga
    12541 agacgaggac atggccgtgc ccggcctggt gcaccgctac caggaccgcg cactgctgat
    12601 ttccaccaca tcctgcgcaa tgtactgccg ccactgcacc cgcaagcgcg tggcgggacg
    12661 aagggacacc tgcatcacgc gtacacgcct ggagcgggcc gtgaagtatc tttcaatgca
    12721 tcccgaaatt cacgatgtga tcatttccgg aggcgatcct ctgaccatgt ccactgaaat
    12781 gctggaaacc gtcatctcag ccgtgcgttc ggtgaaaagc gtggatatca ttcgtatcgg
    12841 cacgcgcact ccggtggttc ttccgatgcg catcacggat gatcttgtga acatgctgaa
    12901 gaagtatcat ccgatcttca tcaacaccca cttcaaccac cctgtcgagc tgacacccca
    12961 ggccatcgag gcctgcggga aactcgcgga tgccggaatt cctcttggca accagtcggt
    13021 gctgctgaag ggcatcaacg atgatcccca tgtgatggaa gagcttcttc gcggcctgct
    13081 gaaggcccgc gtcaaaccct attacctgtt ccagtgcgac ctggtgcgag gcgtggagca
    13141 tttccgcacg cccatctcca agggaattga gattatggaa tacctgcgcg gccgcgtcag
    13201 cggactgggc atcccgctgt atgtggtgga tgcgcctcat ggcggcggga aaatccccgt
    13261 gctcccgaat tacatcgtct caaccagccc gacccacacc gttctgcgca attttgaagg
    13321 ccagatggta tcctatccgg aacccggccc gcataaagcg ccgcacacgg gtaaaacgga
    13381 cagaggagat tccgctgccg gaatatggga attgtcatcg ggtcagcaca ccaagattca
    13441 gccgcagaac tgcgcgcggc ttaaccgccg agagacgcgc aagcagaccg ccgcgatgct
    13501 gtccccggag gcttgagcgc cgtgagccgg cgcggcggag ggaatatgag gttgaagatt
    13561 ggcatgacgt atgacctgcg gtcggattat ctccgcgagg ggttcacaga ggatcaggtc
    13621 gccgaatttg acaccgactc cacaatagac gccatagagg ccacactgcg cgatctggat
    13681 catgatcccg tgcgcatcgg ccacggccgc gctctttgcc agaagctggc gaatggggaa
    13741 cgatgggatc tggtgttcaa catcgccgaa ggcgtgacgg gccgcagccg cgaggcacag
    13801 gtgccggctt tgctggagtt gtattccatc ccctacacca tgtccgatcc gctcgtctgc
    13861 gccgtgacgc tggacaaggc catcaccaag cggctggttg catcggcggg aatcaacacc
    13921 cccgcctacc ggctgattgc aaatctccga gattcacagc aagtggaact ccggttcccg
    13981 ctgttcgcca agccggtggc cgaaggcacc gggaaagggg tggatggctt gtcgcgcgtg
    14041 gataatccgg aacagctcca cgatctgtgc aaggccctgc tccacaagta tcatcagccg
    14101 gtgctggtgg aagaatatct gcccggccgc gaattcacca cggggattct tggcacgggg
    14161 ccacatgcgc gggtgctcgg cacaatggaa gtcgtcatag gagcccacgc accctcccgc
    14221 gattacactt atgaaatcaa ggagcgatgc gaggagtttg tggtatacag accgcttgaa
    14281 catggtccgc ttcggagaga ggcggaagag cttgcgctga atgcataccg cgtgcttgaa
    14341 tgccgcgatg cgggccgcgt ggatatccgc gtggccgcaa acgggcggcc ggcgttcata
    14401 gagatcaatc ccctgcccgg cctgcatccg catcattccg acctgccgat gatcgccacg
    14461 caggaaggca tgagctatcg cgacctgatt gatcacatca tcaaaagcgc agcggcccgc
    14521 gccgggctgg aggcgtagtc ttttgaagac ggtactgatt ctttacaacc agccgcgtga
    14581 atccgcggcg gcaggcgtaa cgcgcgagtc ggacgccggt gtgctggcgg aagtggcggc
    14641 ggtggcaggc gcactgaaaa gggatggcgt cccgttcaac accgcagcca tttcatgcct
    14701 gagcgaactg ccgaaggttc tgaacacatt cagcgatgag attgttttca atctggttga
    14761 ggggctggag gggcccgtcg gagatgtcaa tgccgtcccg gtggtgtgcg aagccttcgg
    14821 gcgcggatgc acaggaaaca caacagaatg tctccatcag agccttgata aatgggtcac
    14881 caaactcctg cttcatgaga aaggtcttcc cgtgcccgcg gcatggattg ttccggtcgg
    14941 acagactttt tcacggccgc cggatatcat gaagccgctt ttgctgaaac ccctgctgtc
    15001 cgacgccagc gaagggatca cccagtcttc ggttgttccc catccttatg cggacatcca
    15061 agaccggatt gcagaacttc acgcccgctt cggccagccc gtgctggtcg agacctttat
    15121 tgacgggcgc gaattgaacg tgtcggtgat agatatgggc cgtggaccgc aggtcatgcc
    15181 catagcggaa attgatttct cggccttccc cgcaaaccgc cacaagattg tggactacga
    15241 agccaaatgg atcgagaatt ccttcgccta caacaacacg ccccgcaaaa ttccggccgc
    15301 ccttccggaa aagacggcaa atacaatccg cgatctggcc ctcggcgcat ggcgcgccat
    15361 gagctgccgt gattatatcc gcgtggactt ccgccttgat gccaacctca atcccttcat
    15421 tcttgaaatc aacgccaatc cggacatttc gcccgaagca ggtcttgcgg ccgcagtggc
    15481 cgcggcgggc attgcataca atgagttcgt gatgcaaatg atccgcaatg cttccggccg
    15541 tcgaattgcg gagaaatcgt ccggaattcg gccggggccc gcaaaaattg acggcggcgt
    15601 tgaaattcgc cggaccatgt ctgccgatgt gaaaccgctg atgaacattc tggcgggaac
    15661 tgcattcttc cacgacaacg aactgcagat tgccgaggaa gttcttctgg attccgtgaa
    15721 tgagttcaag gcatccgatt accagagctt cacggcctgt attgaaggcc atccggcagg
    15781 atgggtctgc tggggcgcga cgccctgcac cgacagaaca ttcgatgtct attggctcgc
    15841 cgtggacaaa aagattcagg gaaaaggcat cgggaagaaa ctcatgacct tcaccgagga
    15901 atccatctgg aaagccggcg gacgccttgc cgtactcgaa actgcaggcc gcgaggacta
    15961 tgaacccacg cgcagatttt atctctccat cggctacacc gaggccgccc gcgtgaaaga
    16021 tttctacgcc ctcggcgacg ataaggttat ctacaccaaa cccgtcccgc gttaattgcg
    16081 gggcgcgaca gggcggcgcg ggaggtctgg ctcgatgcct cgcgccagag actggaatcc
    16141 cggatgctgg cgaatcgcgt tgaagtcgct gtcgcgcaga tactcctgca gcgccgaagg
    16201 cgaacagtgt tccgacccgc ggcggatcca ctccagtgca cttgcggcgt cgttgtcata
    16261 gacataggtg atcgccatgt tgaaatacga ttcagggaca tccggatgcc tcgtcaccag
    16321 ccagtttaaa tgctcgcgcg cttcctcaaa gcgtcccaga cgcaacaggg caaccgcata
    16381 gttgttgcgc agcgatatgc tgtcccgcat gccggccatg gccatttcga aatattccgc
    16441 cgccgattca tattgagccg aggccagaaa gagcagaccg agattcacat agccgatatg
    16501 ataatccggc ttgatctgca ccgccgtgag cagcagttcc tcggccttct cgcggtctcc
    16561 gcgctgaaga taggccgtgg acagattgct taaagtctcc ggattgaacg catcccttgc
    16621 cagggctttc tccagaatgg agatggcctg atcaaactgt ccccgctgga tatagattac
    16681 gcccagcatc ctgagggccg catccatatc aggctgaata tccagcgcct tgcggacatg
    16741 aagggtggct gcatcatagt tcccgccctg aaggtattcc tgcgccttcc gcatttcgcc
    16801 catcgccgca gtcattttct gcggatcaag cggcggggcc gcgtcaccgg aatccacaga
    16861 gggttccgtc cattcacggg gtgcggtata cagcgggccg ggacttgtcg cgggccgatc
    16921 cgtcatcgcg gcgtttgtat ccagactcag caggaggaaa tccttgtctg aatccccatg
    16981 cttccggctt tcagcccaca tccaccaggc cagcagcagg aaaaccacca tcagtgccag
    17041 aaacgccatg gatgtcagcc gtgtgcgccg gtgcgcttcg atcatcgtga tttcggcgcc
    17101 ggcccccgac gcgcctgccg gcgaattccc gcggtttttc ttgaacctgc tcacagtgtc
    17161 ctccagttag agcttcagaa aattctgaat aatcaaacgg cccgattgag taagaatgga
    17221 ttccgggtga aactgcacgc catgcaccgg atgctcggcg tgctgcagcc ccatgatttc
    17281 cccctcctct gtctccgcgg tgatcttcag gcattccgga agcgatgctc tctcaaccag
    17341 aagcgaatga taacgggttg cttcaaacgg ctggggcatg ccccgaaata caccttcacc
    17401 attatgccgg atcggggacg tttttccgtg catcagacga ctggcccgga tcacgcgtcc
    17461 cccatagacc tggcctatgc actgatgtcc gagacaaacg ccgaggaccg gaattttcgg
    17521 gcccaggcgg cgtatgattt cacacgacac tccggcttcg tctggcgtgc atggccccgg
    17581 gctgatcagc gccttctccg gtttcagcgc acacaattca tccggcgtca tggcgtcgtt
    17641 gcgaaccact ttaacgtctg cccccatctc gcccatatac tgcacgaggt tgtatgtaaa
    17701 agaatcgtag ttatccagaa caaggatcat gatttccccc tcagttcacc aaaccggcgc
    17761 gaaagcacaa gcgacttcag catgccgcgg gccttgttgc aggtttccat gtattccgcg
    17821 gcgggatcag agtccgccac aatgccggcc ccggcctgga cgcaggactt atctccgtcc
    17881 atcagcaccg ttcggatggc aatgcaggaa tccaggtttc cggaatagcc gaagtagcag
    17941 accgcgccgg cataggggcc ccggcggccg gcctcgagtt cggaaatgat ttccattgcg
    18001 cggatttttg gagctccgct gactgtcccc gccgggaatg tggcccgcat caggtcatag
    18061 atgtcccggt cagcgcggag ccggccggtc acatcagaga caatatgcat cacatggctg
    18121 taacgctcga taaccatcag ttccggcacc gtcacactcc cgtattcgca cacacggccc
    18181 agatcgttcc gcgccagatc caccagcatg atgtgctccg cgcgctcctt cgggtcggcc
    18241 aggagctccc gggccagccg atgatcctcc gcatcatcgg ccgaacgcgg acgtgtgccg
    18301 gcaatgggcc gcagttcaac ccggccatcc tcgcagcgaa catgaacctc aggcgacgac
    18361 ccgacaatgg cgcgcgaccc gttctcaagg cagaacatgt agggagaagg attaaccgat
    18421 cgcaaggcgc gatacacatc gagagggtcg cccgccgtgc cggtttcaaa gcgctgcgac
    18481 aacaccacct gtatgatatc accggcgcga atgaactcct tggcggtcat cacggcattt
    18541 tcgaattcat cgcgcgtcat gtttgagcgg aactccggct catcggccgc accggacgca
    18601 tccagcatca catggggcac atgcgtgctc aaatcgcggc acagcgagtc aatcgccgca
    18661 acggcatcat cataggcctt ttccggatcc tcttcgacaa gagcattggc gaccactttc
    18721 atggtgtggc gcgcgcggtc aaacacaaca attgtatccg taatggccag catcatgtcc
    18781 ggccactctg tgccgccccg cgcgcaaagc ggcacgcgct cgaactgcgc gactgcatca
    18841 taaccgatat atcccaccgc gccgccataa aagcggggaa gcgacagatc gggcacgggc
    18901 cggtatttgg aaagatgctc cttgagcaca gaaagcggtt cgcgatttcc gatcgtttca
    18961 acattgccgt cgcggctggt gatggtcgtg gtccgaccgg ctgaactcag caccgcacgg
    19021 ggactgcttc ccaggaacga atagcgcgca atatgctcgc cgccctccac gctttcgagc
    19081 aggaaagtct gtgccggctc actgcgggag cgcagcgcct ggcgcatgcg ctcataggcg
    19141 cacaggggcg tatcctggtc tgccagcagc tcgcgccaga ccgggatcag atttccctgc
    19201 tttgcttttt caagaaatcc ttctctggtt ggcgtgtaca tgctctgctc cagtcgtttc
    19261 aggggtccag attttcaagc tttccggtta cgaagttcaa ccggcggtaa tagcagttcc
    19321 gggcggcgcc ctgccggttt tttgtatggc agatgccgcc gcggcgcggg cgcacgatat
    19381 aaaccagcga attctgttcg cagttcaccc tcacctccag cagatcaaac gtctctccgg
    19441 acgtcttgcc cttttcccag agctcattgc gcgacgtgct ccagaaaaca gccgaacggg
    19501 tttcaatgga cttcttcagg gccagctcat tcgcataagc caccagaatc acctcatgcg
    19561 tgtccgcatt ctgcacggcc accgggatga tccccggagc cgcagcggac gccttggcca
    19621 gcttgtcgaa atccagcgcc agttccaacc cttcttcaag ttctctgctc attgtattct
    19681 cccgctaggc gcgaaccgga atgcccctct cgcgcaaata gtcttttgtt tgtttgatgg
    19741 tgtaggttcc atagtgaaaa atcgaggcag ccagcaccgc atccgcccgg ccggccgaca
    19801 gggcttcata gagatgttca aggctgccgg cgccgcccga ggcaatcaca ggaacagtaa
    19861 cagcctccga aacggcgcgc gtcagggcaa gatcgtagcc gttttttgtt ccgtcccggt
    19921 ccatgctggt cagcaggatt tcaccggcgc cccgacggac cccctcacgc gcccattcga
    19981 gcacatccat gccggcaggc cttcggcctc cgtacgtgta aacggtccat cctccgccgt
    20041 cctcgttctt tttggcatca atggccagca ccacgcactg gcttccgaac tgttccgcgg
    20101 cttcgctgat cagcgccggc cgttccacag ccgcggtgtt gatcgaaacc ttgtcagcgc
    20161 cggcctgcag cagaatgcgg acatcctcac tgttcctgat tcccccgccc acggtcaggg
    20221 gaacaaacac ctgccgggcc acccggttta ccacttccac catcgtatcg cgaccctcat
    20281 gcgaggcgcg gatatcaagg aatgtgatct cgtccgcacc ctgttcgctg taagccctgg
    20341 cgcactctac cggatcgccc gcgtcctgaa gactgacaaa attaaccccc ttgaccacgc
    20401 gtccgcgggc cacgtccaga cacggaatta ttcttttagc cagcattgcc gtttcctgaa
    20461 aaacaaaaac cccggttcat ccggggttcc atccatcagc acaagccgaa tttaactgtg
    20521 gaggtcccca gtcaatccat tcttgtgcca ccagttcatt ttcatttatg caccatatac
    20581 ttgaaggccg ccgaggcgca agcggaaagt atctggaagt tgaccgggag ctgctggccg
    20641 gcatagcgcg accgattcct tcacgcctcc ttaatggacc gcgctatgcc gtgatgcagc
    20701 agatttttga gtttgcaccc cggtccgctg ctccagcaga ccgcgcaggc aaacgagaag
    20761 agcttccgtt cccagtggct tcatcatgaa taatgtgcag ccatcatgag ggtacgggtt
    20821 ttccgggaca tccccgctta ttttcaggac gggcatggcc gggatgaatt ttcgcagggc
    20881 cagcatggcc tcgtatccat tcataccggg catagtcatg tccagaatca ccgcgtccac
    20941 ctctgcgttt tgggccacaa acaagctgac cgccttcgaa ccgcttgcag gcgccagagt
    21001 ccgacagccg ttgtgacact atgccagccc gatcagaaac cggacggcgg catcgtcgtc
    21061 aacggtcagg accagttcgc ctttcccctg cggcattctg tctccggccg tccatgcggc
    21121 cggagacagt gcggacttga ccgccccttc atgatgatcg cgagtcaggg cagtgtccct
    21181 atcgcgcctc ccccgaaaag ccggtcgcca acagccacct gtacttcccg ttcatcattg
    21241 catgtctccc ccgaacggcc aatcgcccac aatatgccga tgcataaaac ccggccacca
    21301 ggcagcgata cgcctggaaa aattcatctg ttcagttcct ttgcggccct ccggggcaac
    21361 ccccgctgcg gttttatccc gcagccgcag aaaccaacga cactctgctt caggccgttg
    21421 tgaatccttt ttcatattca tagactaaac agaggaagga accggctgga agtcggggaa
    21481 tcactggctt ttctgtaggt gtttttacct acagattgct caggcgcaca aaagccgaac
    21541 tcaaccggtc agattgtgct gaaatgcata gcgcgtgagc tgcacattgg tggaaagtcc
    21601 cgttttctcc gcaatgcgag cacggtaggt cgcgattgtt ttttcgctga gggaaagctc
    21661 ttcggctatc ttcttggccg ttttcccttc agcgatgagg cgcagcactt gaagttcacg
    21721 ggccgaaaga gtttcgtgag gaagtcgagc gtcactgctg gccgtcagga ccgtcaccag
    21781 cttttcagca agtattgcgt tgacatacgt gccgccgccg agaattttat tcacggcctc
    21841 cacaagtttc tccggcgctt cattctttcc gagataaccc gccgcgccca gcttgagtgc
    21901 gcgaacagcg aattcttcct ccggataagc gctcaccacc agcacccggg ccgccggccg
    21961 aatccgccgg acatcatcaa gcaggctcaa tccgtcccgt cccgggatgt tgatgtccag
    22021 cagaacaaca tcccacggct ggcggcgaaa tgcctgcagc gcggaacgcg attcgtccgc
    22081 ttcgccaaac acggcgtcgg ggaactcctc ggaaagaatg tctctcagcc cgcgacacag
    22141 aatcggatga tcttcgacaa tcagtatgcg caggcgattg ggcgcggccg gttgtttgtt
    22201 catgagcccc tcttcctgga gcggcgagaa ccgccctttg cccggctcag aggaatgcgc
    22261 acctcaacca ctgtcccgtt cttgttcccg cggcgaaacg cggcctcccc gccaagagca
    22321 acggcacgct ctctgattcc aatcagaccc agcgaggcgg ggttgttcac cgccgcttcc
    22381 ccaatccccc ggccattgtc ccggacacac agtttcagac ttctgtcagc catgcccaga
    22441 cggacagaaa cccggctggc cccggcatgc cgcgcgatgt tggtcaagca ttcctggaat
    22501 attcggaaca gggccgtggc gacaagaggg ggcacttccg acaaccggga agccacagcc
    22561 atggtacacc tgattccatg cctctcttca aaacaacggg cctcatactg caacgctgtg
    22621 cacagcccca gtttgtccaa aaccccaggc cgcagttccg cagcgagttt ctgcaccatg
    22681 ctcagggcgg aatccgccag actgatagcg ccagaagccc gcttttgtat tttgtcctgc
    22741 aacagggccg gggaaatctt ctctgctaac ttctcaattc gtcttaagtc catcttgata
    22801 gctgtaaggc tctgtccaaa gtcgtcatga atgtcgcggg cgatgcgtat ccgttcgtcc
    22861 tcccgggcct gttccaggcg ggacagcaga gcgcgcagtt cctcccttga ccgggacagc
    22921 gcccgctctg atgcccggag ttctgctgtg cgctcctcaa cacgccgctc gagttcgtca
    22981 tgcgctcccc gcaacaactg ctcggcctgc ttgcgctccg tgttgtcacg gataacggcc
    23041 cacaccccgg ccggattccc cgcctcatcc cgaagcagac agccgcgcat ctccaccgga
    23101 acgcgagtcc cgtccttgcg aatgaattcc ttttctatga tgtcggtata gcctcgctcg
    23161 gccacctgcc tgaacgccat ctcatccgtt tcgatccatt ccggaggagt gaagaccttg
    23221 tagctcaaac ccgtgacttc ctcttttgaa tactgaacca gccgctcaaa ggccggattg
    23281 gtttccagga gccgcccttt catatccgca ataataaatg cgtcctccat gttctcatac
    23341 agcagacgat attttgcttc cgaccgccga agggcctcct ctgcctgttt gcgctccgtg
    23401 atgtccagct caatgccatc ccatatcaga tgcctgttag gaagccgacg gggggctgaa
    23461 gcaaaccatc tccagcgctt ctctccagaa gcaagccgaa tacgcgcttc cacattaaat
    23521 ggagtcattg attccgccgc ctttgcttca agtccagcca ccagttcttt gtcgatgacc
    23581 tgccggtaaa tggcccctga atctttcatt acttcatctg tcgtgacacc gtgcagctgc
    23641 tcaacgccct gactgacaaa tgtgaagcgg cgcgactggc cgtcctctcc ggaatcgatt
    23701 tggtaaatga gtccgttcgg cagattgtca tgaatggcgc ggtggcgtgc ctcgctctct
    23761 ttcagcgcct cttccgcctg cctgcgctcg gtaacgtcta tcaccatgcc gtgggaacaa
    23821 accacccggc cctctttatc ccgaacgtga aaacacttct cttccaccca tcgcaccgcc
    23881 ccggtatccg cgcggacaac ccggtgctcc atttgataag cctcaccgcc ttcacacagt
    23941 gaattcctgt acgccatgtc aagcgcagca cggtcttcgg gatgaacaag ctcgagaaac
    24001 gcctcgtagg ttgccgcaaa ggactgcggc ggctgcccga aaatgcggaa cacctcatcc
    24061 gaccagatca gccggttgct catcagatcc agttcccagc tccccagatg agcaatggcc
    24121 tctgtccggt tcaggcgatc ccggctcgca cgcagatcgt cttccgcctt tttgcgccag
    24181 gtgatgtccc tcgcaataaa gaccagtttg tttgagtccg cggggctttg tccctgatcc
    24241 gcaatggcca gctcaaacca gcggtctcct tcgggatatg gaagggaata gacggtttct
    24301 ttgattccgc cgaatttcag cgcatggttt attgcctcgc caatgatctc tgatgccgga
    24361 tgagggagaa ttttccggat atcacggccg agaaactcat ccgggggcat atagagaagt
    24421 tcagggtgca cgacgaaata gtctgtaatc acgccagaac gatcgagcac aaacaagaag
    24481 tctgaaaacg cgctgcccgc cgcctcaaga cattcgcgcc cccttcccga aaaatactct
    24541 ctcgcagcaa cagtcttcct gttccgcgac tcaaccggag attctttact cattatattc
    24601 ttgaattttg tctcacagaa tatgctatgg cgcggttaca gtcaaatccc cgcatcgggg
    24661 tcccgctccg gccgccccgc ttccggaagc acgggatacg gcaggccgcc tccggcggca
    24721 gatgcacccg gaggcggcgc aggcgacgcc gttcccgcgc cgcaatgtcc gccagtttgg
    24781 ttatggcgcc ttccccgaaa agccggcaaa aaccgcctgt gcttccccgg cgacattgca
    24841 tgctcccccg aaacggcggg tcacctgcaa gcgaccggaa acagacccgg caactatttt
    24901 gcatttttac cagacagggt ccgaaaggag tatcgcggga atcacttttt cggggtggcg
    24961 gaaaacaggg ggctaacgag cggcttcttc cgccagaaat cgggccgcgc tggccgccag
    25021 ctccgccgcg ttccgcaaat ggagtttcag cttaatgctg gcccgatagg actccaccgt
    25081 gctcacagac aaacccagcc tggccgcaat ctcccgggtg gcgcggccca gaccgatcgc
    25141 gcggtagatt tccagctcac ggttgctcag cagttcaacc ggagaccggg gcagcgaggc
    25201 atcctttcca atgccaagtt ccatcacctg actgatcacg ccttcgctga aagccatttg
    25261 gccgttgagc agtctcctta gcgcctccag cacacgggcg gacggctcat gtttcattac
    25321 ataaccgcgg gcgcccgcac gatatgcgcg aacggcgtaa acatcttcag gatgggcaga
    25381 cagggcaagg atggggagct tcggctgttc cgcatgaagg cattcaatca tttccagacc
    25441 gctgccctcc tccagcgtca ggtcggcaat gaccacatcc ggtttcagct ttctcgatgc
    25501 cgcaagtccg ccggccgcat cggccgcagc accgcacatc acaatatccg gctgccggtc
    25561 aatcacccgg cccagaccct cgcaaaacag aggatgatcg tccacgatca ccactcttct
    25621 cttaccggat gcagcattct tcattatagc ccttctgcac agaagtgggc agacattccc
    25681 cgttcccgcc gattaatcac ccaaaactcc atcatgagtg tactcgccta aattggggtt
    25741 gtcagcccca aattgacctg ttttggtgaa ttcagccggg cccccggttc gacgcagcgc
    25801 cggcagagaa actcaccgcg ttcaaatttc taccgccccc tcccgaaggg cctgccgcgt
    25861 ggattttggg cgggaataaa aatggtcggg gcgccgagat ttgaactcgg gaccttctga
    25921 accccattca ga
//
LOCUS       DAOQWR010000019        25400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2274_length_25400_cov_5.517775, whole genome shotgun sequence.
ACCESSION   DAOQWR010000019 DAOQWR010000000
VERSION     DAOQWR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25400
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2274_length_25400_cov_5.517775"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="PKK36_02875"
     CDS             complement(<1..1610)
                     /locus_tag="PKK36_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93533.1"
                     /translation="MFVKKTLAHKIRILGIAGILLYVLVSTAVIAPYVLRQYSASVLE
                     KDRIIVKGLAGFMEEFLGQAHMLNRNLSRHPDVIRNILESHPDMEERKKIYAEKYDVT
                     IPCGEGSGFPLFVDQQLRYPFVELLYVQDIRGNQSARSHHQLGPRGTRWYFQRMLNDP
                     LHRPFVSKSYYSVTSEEPTAAVFHPVEFNGELIGIMGADINFRAMQSAIDAFLGVKDL
                     YAMMVDQEGAVIAHPDKTRVNEMHNLIQMKKHILVRDEQGRVHLDEQGYHVVEGVKLE
                     RGCPALAECIQQAVRGKSGWHSGIRLDEKITTVYYEPVLLPGKRSATEYYAVLLVRDH
                     STLLATRRMAMLLVAGIAGLVGLALHAGVGWLFRRQIYRPLSRLSAAMEQTEMGRPVK
                     ADIRTGDEFEALADTYNRMQQRLQEVTAALQQSEEAYRSLYENVPVGILRIGLNGAGP
                     VMQANPTAARIFGFENSDRMMNQDMEPLCTDREEYREYVRRVLQHGAEGSCELRMKTQ
                     KGGMIWVSLTMKVRRAGSGAPQWVDAVL"
     gene            complement(1804..3018)
                     /locus_tag="PKK36_02880"
     CDS             complement(1804..3018)
                     /locus_tag="PKK36_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR93534.1"
                     /translation="MAYDIIIRNAQLRRKPGTWDIGIENGIITKISKKIQGRGKKTIN
                     AGGNLVTESFVDPHLHMCKVYTTQMLDAKALQAYHGGNMGGAMTAIEQAARVKENYNE
                     KWIVPNVIKCLKKAALNGKTHIRAFADVDSKAKLIGVKSLIKCRDMFKGIVDVQVVAF
                     PQDGVVREPGTVDLIKQAMDLGADVVGGIPWIEFTDADEQTHINEMFRIANDYGADIS
                     MLVDDAGDPGLRTLEMLATKTLEEGRIGRSLAHHARAMCMYNEPYFRKLLALLQKAKL
                     GVVSDPHTGPLHARVKDLLAANSRVCLGQDDVADAYYPFGQNSALEVGFLNVHLLWMT
                     TFPEMEDIYDMITTRAAECMNMQKFKVAEKCPANLVVLDAKSVYEALWYHKEPLYTVS
                     HGAVIDKDRMRK"
     gene            complement(3043..3525)
                     /locus_tag="PKK36_02885"
     CDS             complement(3043..3525)
                     /locus_tag="PKK36_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR93535.1"
                     /translation="MSEKITLTVNGTKRSVEIDGWERLLDVLRDKLDLTGAKRGCDDV
                     ACGACTVVVNGEAKKSCSLPAKRCDGAEIVTIEGLASGTEVHPIQQALIEAGAVQCGY
                     CTPGIVMSLYALFTKNLNASEDEIEDVLEGHLCRCTGYEAIWEGALLAQKLLKKNKKK
                     "
     gene            complement(3529..5850)
                     /locus_tag="PKK36_02890"
     CDS             complement(3529..5850)
                     /locus_tag="PKK36_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNR93536.1"
                     /translation="MEFKYVGKSITRPDAVKKITGKAIFLNDIKLPGMLHAAILRPDY
                     AHAKILSIDTSAAEKCEGVVKVVTGRGCKILYGDNIRDLVPMAVDKVRYIGEPLAAVI
                     AENPYQAKQALKEIKVEYEPLPVLTDALQALEKDAVLVHENSGDYWHLPGLGPIQGTN
                     IANLYKLKKGDAEKGFKDADVVVEAEFNYPHGSSCAIEPHGAIAWFHEDKTIECWSSS
                     ICPFIIREELARVYGRPSSDVRVHIPELGGCFGYKSDITVEQTVAYIASFVPGRPVKW
                     VASRREDFTSTLLGHGIRTRMKIGAKKDGRLTAMETRVYHSTGAYADTGIHVCIAAGH
                     NSAGAYEIPNCDLQAYLMYTNQPAVGAYRGYGHQESQFATERMMDMLARKLKMDPFEL
                     RAKNYLGPGKKTAVGETLCEVNGDVTKCVDIVKKAIYGKKQEKKEDDKYFYGRGFAAL
                     MKTPKGAPNSAKGCYLKFNQDGSVCINMGGAEVGQGLRTVVQQVAAECLMIPPERIKV
                     YTEIDTQFSPWEWQTIGSMFTTQGGRAVVRCCEKAIKMMKKTAAQALRVDLDMLDYNG
                     KCVYLKNDPTVNVPLEGLVYGYMYPNGMTVGEVVQSSDAARLPRYSNPDENGQGTMGV
                     SHTFGAQGCEIRIDKETGEVLVDHFASAFDVGRVINARQIRGQVAGGVMMALGATLHE
                     ELKFDKDGKMLNPFMKKYRFPTIKNRPKKQTIEFVETPETIGPFGARGIGEHPVIGVA
                     PSILNAIYDATGIEFTQIPVTPEKMKEALKARK"
     gene            6035..7255
                     /locus_tag="PKK36_02895"
     CDS             6035..7255
                     /locus_tag="PKK36_02895"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HNR93537.1"
                     /translation="MKNKISSEFIRAIPKTDLHVHLDGSLRLSTLIELARAEKVELPS
                     YDEEGLRELVFKNAYKDLVEYLKGFAYTCAVLRTAENLERIGYEMVQDNIAENVRYLE
                     VRFAPQLHIHEKLSLEEIVTAVCRGMSRARDEHNRSKAVKDGTDIPFFFGVIVCAMRC
                     FNEHYSPYYAQLCSAMKYAPEKEIFATASLELARAAEILAHDRGLPVVGFDLAGAESG
                     YPAISHAEAYKYVHSHFIRKTVHAGEAYGPESIFQAITECHANRIGHGTWLFAHDRIS
                     NPDIADPEAFVHYLVEYIASQRISIEVCPTSNLQTMPELKNDIRNHPLKKMLEHELSV
                     TICTDNRLVSSTTTSDELMLVTEALDLPLRDVRNLVIAGFKGSFFPGTYPEKRAYVRK
                     VINQLDRLEQEMLG"
     gene            complement(7263..7631)
                     /locus_tag="PKK36_02900"
     CDS             complement(7263..7631)
                     /locus_tag="PKK36_02900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNR93538.1"
                     /translation="MNGPSEDRQNVRIIKTGAVRMVWRRAFFRRKMDGNIVDVSARGL
                     QVSAPSPLPEGRKVKLDVRLFSGPQERQRYRLYGRVIRFAPDSISGRWLIGIALTSVR
                     GDRRAWNSAIFAFLRSRNEF"
     gene            complement(7676..8065)
                     /locus_tag="PKK36_02905"
     CDS             complement(7676..8065)
                     /locus_tag="PKK36_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNR93539.1"
                     /translation="MNPASDRRRQARIRTAGSVTLTLRRGFFGHRILSGSLVDVSVDG
                     LQVQLPAPVAAGRRVKVLLALGVPGSEAAIYRFPGRIIWSKAAGPDGYSLAGVRLRVR
                     SRNLRGIWQNVIYEALRTPAGSRANNP"
     gene            complement(8062..9012)
                     /locus_tag="PKK36_02910"
     CDS             complement(8062..9012)
                     /locus_tag="PKK36_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNR93540.1"
                     /translation="MKIVVLDGFTLNPGDLDWKRLHALGECHIHDRTPPEMVPERSAG
                     ADVILTNKTVLSGDAIRKLPALKYIGVLATGYNVVDVDAARERKIIVTNIPDYGTASV
                     AQMVFAHILNLTQRVADHSRGVHAGDWSASEDFCYWNFPLIELQDLTLGIIGLGRIGQ
                     AVARLALAFGMKVLAQNPSPRTMSGVEPADLNTVLSKSDVVSLHCPLTDANRGFINEQ
                     TLSLMKPTAFLINTGRGPLVDEAALAAALRAGKLAGAGLDVLSVEPPPKDNPLLNCPN
                     CFITPHIAWATRAARIRLYDIAVRNIEAFMAGKPQHVVNP"
     gene            complement(9020..9430)
                     /locus_tag="PKK36_02915"
     CDS             complement(9020..9430)
                     /locus_tag="PKK36_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HNR93541.1"
                     /translation="MKNMMVTLALILLAGCARPRHPEEAAAMYAAQAWLQEVDSGAYA
                     ASWSNAAAYFQGAVAESDWAGMMTSGRAPLGEIISRDFKSARHFTSLPGVPDGDYIVI
                     QFKTSFARKKNAVETVTPMKEDDGEWRVSGYFVK"
     gene            complement(9534..10202)
                     /locus_tag="PKK36_02920"
     CDS             complement(9534..10202)
                     /locus_tag="PKK36_02920"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNR93542.1"
                     /translation="MLTFREFKTEKSLARRVRQKLQQVIESARGPSAIMLSGGRTPLP
                     VYRDLASRKIRVSPDVHFFLSDERMVPPDSPDSNQGAIAPLLVKAGAAEDRFIRVNTS
                     HPLVMAAAEYGARLEDFHRAGGKFPFGLLGLGSDGHTASLFTLEDARSETGYFAIPVE
                     RIGDFDRVSVTPAMMRNIGQIVFLVAGADKAVMLEVLRTAPETIPAGAAVRECANAEV
                     WFTA"
     gene            complement(10206..11660)
                     /gene="zwf"
                     /locus_tag="PKK36_02925"
     CDS             complement(10206..11660)
                     /gene="zwf"
                     /locus_tag="PKK36_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNR93543.1"
                     /translation="MNAQNGNLSFIVLGASGDLARRKVIPALFALYCQNLLPANFQVL
                     GFARTKMTDEAFREMITAHLTCRYTPGEACGERMTEFLARCFYRSGRYDSRESFQALY
                     GTLRELEGPQEACRIFYFAVPPAIFHDIAHALGGAGLVRPAGSQPWTRVVIEKPFGTD
                     RASSDELSQQIKTVFDERQIFRIDHYLGKEVIQNLMALRFANLIFEPIWNRLYVDKVF
                     ISWAETIGIEGRAGYFDGIGVIRDVMQNHLLQMLALVAMEQPVSLTKYVRDEKVKVLR
                     SILPVTIEDLVLGQYAAGGGQKGYREEPGVPADSITSTYAMAVLRVRSRRWSGVPFIL
                     SAGKALDRNMTEIRIQFKRTAGHVFLDKWPEGLPPNELVIRVQPDESIKFGIMNKVPG
                     LGLNLEHTELNLRYASAFDGLIPEAYESLILDVIRGDPGLFICEDELEASWDVFTPVL
                     HEIDEKRIEPEPYAFGSAGPAGIQKMLSKFPPRW"
     gene            complement(11678..14704)
                     /locus_tag="PKK36_02930"
     CDS             complement(11678..14704)
                     /locus_tag="PKK36_02930"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNR93544.1"
                     /translation="MPSAPVRAVRWAAAAVAAAYLALAVFVSPEWIFEGRPGEEQRLR
                     ERLSRALASRSGLLHLITFDEPAPCDFITGHPLLCPGTTSAPGRFGAARKFDGRERTY
                     AESRLRWDRIGSSFTLSFWIRMPEGRPDQCIWYRAGKNVQVGFHLEAGRMTFDMPTPG
                     GRQRASYPFVRYGEFVHLAATVDAERGEIILYENGSPMAREPFVFHGMPRENMAFGKP
                     VWYAARNSFRGWLDEASVWNRALSEQEIRGLARAKRGLLWTGRSGRRCFKWRAAQTGS
                     RILRVSAALPDVFAPLTRRGRSEARAISRLDELRLILPGKVRRELIAAHHRSLKSGRR
                     TQAGARPRQVHVAFRGEVYPALICLSGSDREYHSGKRPGYELQLLEGAQVLGARRLLL
                     SPPENGHWLFPVFDARLRHRLGLPSISGGLCRLRINGLSRGVYAWSVHDRLGLLPGDL
                     PDVCMGAVRHPSQWWLPFHEALRPSWESSASRAAWPLSSAEMKLLYDEVVGEYGGCLR
                     GDLQNPLSRKEISWRLARGRQQIESIWQPAPEHLSPARRAAGFLDEFMVLGSNASPDR
                     IVAPLQLDLAALRNLGVEIRWSSSNPDVLDHAGHVARNFTGGPVRVVLTAAVEDGTES
                     VEKVLTFRVMPRRIALPAIFLNVRHELDKSRRADAAVEFCGAGEDFPSRIRYATQGTR
                     GGMEHRGNSSYWNPKKLFSLKMDEPHGFLDESGSRVLLAVNSQQDPSFVRNGLAYDLF
                     RSWAGEGAPRFAPQVRHAEVFVNGRYQGLYELASRVDETLLNGGSPPADAHKWIIYRH
                     ETVHPRIPDMRARRPAGHEGEFSGPYRSFCRFLEQPGSESWEPELARTMDIPNLADYQ
                     LLLNLFQNRNGYPFDFLLHEVLVCERDRPFFFHVPWDFELGAAPGQWEWLASGLMQRL
                     ENESPRYRGLLSARWRELRESGLTPESLNARIDAVAENLRDYADWDYRRWRYHAGRDY
                     GLWVNNLKAVLNESIERMDLHLDFPPVSGSLPTDPE"
     gene            complement(14706..15401)
                     /locus_tag="PKK36_02935"
     CDS             complement(14706..15401)
                     /locus_tag="PKK36_02935"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNR93545.1"
                     /translation="MKQIRLIDALSAYEQISENLNLAVFAGALLVALLASFLAMGLYR
                     CFYESRGTGSQVARSFPLLGLAVTTLFICIQVSIPLSLGLLGSLSIIRFRTPIKEPEE
                     VGFIMLVIASSVAAATYNFGFLAVLYVLAAASLVLARWFRTLGFLKRDGLLVVTAAAG
                     GSFSSDELSKLLKSHFRHHSVQSVGVRDGKVSVQVTFSGLKTEAVRLQEEITQSFAAD
                     SIHVFLDRPGGIR"
     gene            complement(15405..16124)
                     /locus_tag="PKK36_02940"
     CDS             complement(15405..16124)
                     /locus_tag="PKK36_02940"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HNR93546.1"
                     /translation="MFQLSEESRGARVRELKYVAPAEALAAAEAALDLHCLPDPEHPE
                     GIVESIYFDDDFFSSYWEKVNGDSYKRKVRLRWYAEGIPPTGCGEIPAFLDVKDRMGA
                     LREKRHVAFRANKELLCRARLRDPALRDLLRGAAMAAGLPFTDALVPTIGIRYRRRRY
                     VCPWTGARINLDSDICSLRANTDLFSGEAPLGSERIVCEAKAPAAREWPWGETLMRLG
                     FRMQSFSKYGLFMEMRMEGSI"
     gene            complement(16256..17083)
                     /locus_tag="PKK36_02945"
     CDS             complement(16256..17083)
                     /locus_tag="PKK36_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNR93547.1"
                     /translation="MKSAGLFLGILLAASAASAGSFDFSEYSRLLSRTVKPGGMVNYW
                     GLQAGQSALDEVIEAMQKISLDEFAAWPEAERLAFLINAYNARVLQIISRQRHVLFRT
                     NGWFQKGLSVRRYDGFFDEIPVRLLGRDRTLDSLEEELRRYYDEPGVHMALVKGAKGS
                     PPLRPEAYTAEKLPEQLRDQTENFLRDLRNFQVSVGKKRIRLSPVFKELCGDCISRYS
                     DIITAGDFSPADRAMLGFIASHVNGATREFLLTGDYSISWLKFDWMLNEEDPPARAK"
     gene            complement(17097..18122)
                     /locus_tag="PKK36_02950"
     CDS             complement(17097..18122)
                     /locus_tag="PKK36_02950"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNR93548.1"
                     /translation="MKTVRIFARTMAAAALAFLAGLVILTGDPGGGSKPMKRDNPKTD
                     AELKKMLTPEQYRVTQQDGTERPFQNAYWDHHEDGIYVDVVSGEPLFSSRDKFDSGTG
                     WPSFTKPLKPEGVVEKSDFKLGMKRTEVRSSSANSHLGHVFDDGPEDSGGMRYCINSA
                     SLRFVPAAELEAQGYGEFAYLFADSRTLETATFGSGCFWKPQALFSRLPGVVSTAVGY
                     MGGTVKNPTYEQVCTDRTGHAEVVQVRFDPAKVSYEELLAAFWAMHDPTQLNRQGPDR
                     GTQYRSVIFCHSPGQMEAARASLEKQQQANRKKIVTEISPASDFWLAEEYHQQYLMKK
                     GQTSCSF"
     gene            complement(18188..19078)
                     /locus_tag="PKK36_02955"
     CDS             complement(18188..19078)
                     /locus_tag="PKK36_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNR93549.1"
                     /translation="MSEEFSPFAVMQEPGHCGPCSLASCLFMLGIEATQRDVAWAAGK
                     PYKVFKEGLDEKELAHAARKYAAMTEQILIPCRDEGHVFLRNVLGHLTGAGPAILLVN
                     DFEHWVAVVGYLERKSQFVIYDPKADKPFFRWGHARLLREAWCESDDRREPSQYFSIL
                     IRRKDGSKPVWRITEPWMRIHNRGSACTAGTIATDLERLISNARYPSPRRPKSHRATR
                     TLAMVLRKYRNQVIHAILTWGEGSPDREIRDLRSLYSDYITCADACSLTFPDNTDHAA
                     FVAGLTALLSAYWWGGEMEE"
     gene            19489..21579
                     /locus_tag="PKK36_02960"
     CDS             19489..21579
                     /locus_tag="PKK36_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNR93550.1"
                     /translation="MKSSENNGTRRGASGRLAADEHPHAPLLPSRPRRESRHGKTRAD
                     PYAWAANAGDPELRNFLRASNLHARQSLRPFSGLKRQIESELRALTPTLEGGAPFRFG
                     RHVYHFMHPGDRDYPVLLRWPLHAPNRTETVLDINVLARGRRFLAVPHFALSPDERLL
                     AYAADTTGNRRYRIHVRDLGTGRDLPDIIRNADCFFAWSRDGKTLVYSALDPVNQRPA
                     AVRLRTLGKAARTDKTLYTESDEEFRCILMPTTSGQYIRIVCFSADTEQTLLLKADRP
                     LEKPVAAARRTPRCVDDVDHGGGLFVIRTNRRRSEFSLFTATLPDLAKGRWRALAPPR
                     QGTFVERMSAFREHAVVTERQSACQRLRIISLKTRALRTLAFPERVGEAWVEPVPGMS
                     GSVARLGYTSLVTPPTFYDCALRTGKLRRVQQELTPGYEPAGYETWREWATARDGTRI
                     PLTLAGRRENRRSGNRPVLIFAYGAYGTNIPPWFNQARLPLLNRGFLYVMAHVRGGSE
                     LGEAWHAAAMRRRKGAAARDLNDCARHLIRTGRADRNRIFAETLSAGGITLGGAINEQ
                     PGLFRGIIAHAPFVDPAGSLSDPDIPLTLSEYAEWGNPARAADWRAMCAYSPYDNVTQ
                     CRHPAMLVTCVLKDSQVPFWEPLKWVARMRRADRGGGPILLLTQHHADHSGPSGRTRM
                     MREQAFEWAFLLSL"
     gene            complement(21661..22167)
                     /locus_tag="PKK36_02965"
     CDS             complement(21661..22167)
                     /locus_tag="PKK36_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNR93551.1"
                     /translation="MRAVRADITTLETDAIVNAANTSLLGGGGVDGAIHRAAGPELLR
                     ECRTLGGCATGDAKITRGYRLPAKHVIHAVGPVWRGGNSGEQDKLAGCYRRSLELAAE
                     HGLKSIAFPAISTGVYGYPVTAAAEIAVKTCGEFLRDNQSEMEVIFCCFSQRDLEIYQ
                     NLLAREMR"
     gene            22303..24441
                     /locus_tag="PKK36_02970"
     CDS             22303..24441
                     /locus_tag="PKK36_02970"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNR93552.1"
                     /translation="MVVQLGRIVLFTAVLTLAGAQSLFALAPVTDDVADLYSRAARIA
                     VRRLPQMHLNQQPCNDFVATNALETFLTMLDFDHSLFLAEDVSAFRREAKDLDNRLAR
                     GDLDFAFKVYDLLMVRLSNRVEFVNRMLDKGFDLTVDETYAWDRRHASWPSGTNEWND
                     LWRRKIKNIYVGRVAARLLGEEKKAAAEQDGEEPDGDEALDEELDLSPEDFIRKQFQQ
                     QITVFNDNDAHWLLPLYLSSFTRIYDPHSDYMSANHAEDFDIQMKLSLVGIGALLSTE
                     DGAAKVERLIPGGPAELDGRLKAGDKIIAVAQGDEPAADVLHWPLSKTVRLIRGEKDT
                     KVVLTVIPKSDISGATTRKIDLIRAEVKLEDSAAKGSVENINGLPVGVIRIPEFYADI
                     RGQRRGDSEARTVTRDVRAILDGFRTNDIRGLVLDLRNNGGGPLYEAMELSGLFIEKG
                     PVVQVKSSQGISILRDPDPALVYSDPLIVLVNRQSASASEIVAAALKDYGRAVIVGDT
                     KTHGKGTVQTLIDLQRSKPELGSLKLTTASFYRIAGGSTQIEGVKTDIEIPSMLDYLE
                     VGEEYLPHALPWSLIKPASFSRFDHLQDILPELQKRSEKRRAEDPRYAAYRELLTYLG
                     EKRASKEFSLKLDDRLAMARREDEMSAYLNDVFREDDEDEDKDAEDDEDQADLILDET
                     LLILADLITLSEEKRQVPDDAQPAAEVAKE"
     gene            complement(24530..25372)
                     /locus_tag="PKK36_02975"
     CDS             complement(24530..25372)
                     /locus_tag="PKK36_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNR93553.1"
                     /translation="MELILGQKCQKCHFFDFAFRNAAVIRWPGRPNEWGQETKLTPQD
                     GAQSNYFGFSVSLDGSVALIGATGNNAYRGAAYIFERNTGGTNAWGQVVKLLAPEGAS
                     GDGFGNAVSVAEYLALIGAEWDDLGGILDSGSAYVFHRNAAGPDAWGLVAQLTASDAD
                     DWDRFGHAVSLNGDIVLIGAPWETIYEGKACVFERHFPVPETAIEEMQIQGPEVVCAW
                     TSEVPVDYTLQVSESMDDGASWSNLTGSVDLPGVDGMITVTNDLDAGPQRFYRVKTVN
                     SPIR"
BASE COUNT         5143 a         7992 c         7280 g         4985 t
ORIGIN      
        1 tccagcacag catcaaccca ctggggagcg ccacttccag cgcggcggac cttcatggtg
       61 agactgaccc agatcatccc gcccttctgc gttttcattc tcagttcgca ggaaccttcc
      121 gccccgtgct gcagcacccg ccgcacatac tcgcggtatt cctcccggtc cgtgcacagc
      181 ggctccatat cctgattcat catccggtcg gaattttcga agccaaatat tctggcggca
      241 gtcggattcg cctgcatgac cgggccggcg ccgttgagcc caatgcgcag aatccccacc
      301 ggcacatttt catagagaga gcgataggcc tcttcgctct gctgcagcgc cgccgtgacc
      361 tcctgcagcc gctgctgcat gcggttatac gtgtcggcca gcgcctcaaa ttcgtctcct
      421 gtgcgaatat cggccttcac cgggcggccc atttccgtct gctccatggc ggcggacaac
      481 cgggaaagcg gacggtatat ctgccgccgg aagagccagc ccacgccggc gtgcagcgcc
      541 agacccacca agcccgcgat gcccgcgacc agcagcatcg ccatgcggcg cgtggccagc
      601 agggtggaat gatcacgcac cagcagcacg gcgtaatact ccgtggcact gcgtttcccg
      661 ggcaggagaa caggctcata atacaccgtt gtgattttct catccagccg gatgccgctg
      721 tgccaaccgc tcttgccacg cactgcctgc tgaatgcatt ccgccagcgc cggacatccg
      781 cgctccaatt taaccccctc gaccacatga tatccctgct catccagatg cacccgcccc
      841 tgttcatccc tcaccaggat atgcttcttc atctggatca gattgtgcat ctcgttcacg
      901 cgggttttgt ccggatgcgc aataaccgcg ccctcctgat ccaccatcat cgcatagaga
      961 tccttcaccc ccagaaaagc gtcaatcgcg gactgcatcg cccggaagtt gatatctgcc
     1021 cccattatgc caatcagctc tccgttgaat tccaccggat ggaacacggc cgcagtgggc
     1081 tcctcactcg tgacagaata gtaggatttt gaaacaaacg gacggtgcag cggatcgttc
     1141 agcatgcgct gaaagtacca gcgcgtaccg cgcggaccca gctggtgatg gctgcgcgcc
     1201 gactggttcc cccggatatc ctgcacatac agaagctcca caaaaggata tcgaagctgc
     1261 tggtcaacaa acagcggaaa accgctgccc tccccgcagg gaatggttac atcatatttt
     1321 tcagcatata tttttttccg ctcctccatg tcgggatgag actcaaggat gttgcggatc
     1381 acatccggat gcctggaaag gttgcggttg agcatatgag cctgcccgag aaattcctcc
     1441 atgaatccgg ccagaccctt tacgatgatg cgatcctttt ccagaaccga cgctgaatac
     1501 tgcctcaaaa catagggagc aattaccgcc gtggaaacca gcacataaag aagaatgccc
     1561 gcaataccca gaatcctgat cttatgtgcc agcgttttct tcacaaacat cggcgtctcc
     1621 gaatgtctgt tataactcgc agattttccg gtccaagtca agcccttgac ccggttcgag
     1681 ggattttaat atcctcatgc gtcggaacgc gttccgccgg ccacgctcaa ctcacggact
     1741 tgcaggaaac aaaaagggga acccgccggg gcggattccc cttttttatt cttccgcaac
     1801 aatttacttc cgcatgcgat ccttgtctat caccgcgcca tggctgaccg tgtacagcgg
     1861 ctccttgtga taccacaggg cctcgtagac ggacttggcg tcgagcacga caaggttcgc
     1921 cggacatttt tcggccacct tgaatttctg catgttcatg cactcggccg cgcgggttgt
     1981 gatcatgtca tagatgtcct ccatctcggg gaaggtggtc atccacagca ggtgcacgtt
     2041 caagaaaccg acttccagcg cgctgttctg tccgaacgga tagtaggcgt cggccacgtc
     2101 gtcctgccca aggcatacgc gggagttggc cgccagcagg tccttcacgc gggcatgcag
     2161 cggaccggtg tgaggatcgc tgaccacgcc cagcttggcc ttctgcagca gcgcgagcag
     2221 cttgcggaag tagggctcgt tgtacatgca catggctcgc gcgtggtgcg ccagcgagcg
     2281 gccgatgcga ccttcttcca gtgtcttggt ggccagcatt tccagcgtgc gcagtccggg
     2341 gtcgccggca tcatccacca gcatggaaat gtccgcgcca taatcattgg caatcctgaa
     2401 catttcgttg atgtgcgtct gttcatcggc atccgtgaac tcaatccatg gaataccccc
     2461 tacgacatcc gctccgagat ccatcgcctg cttgatcaga tccacggttc cgggctcgcg
     2521 caccacgccg tcctgcggga aggctacgac ctggacatcc acgatgccct tgaacatgtc
     2581 gcggcacttg atcagcgact tcacgccgat aagcttggcc ttggaatcca catcagcaaa
     2641 cgcgcggata tgggttttgc cgttcagtgc ggccttcttg aggcacttga tgacattggg
     2701 aacaatccac ttctcgttgt agttctcctt cacgcgcgcg gcctgttcaa tggccgtcat
     2761 ggcgccgccc atgttgcctc cgtgataagc ctgcagggcc ttcgcatcca gcatctgggt
     2821 ggtgtacacc ttgcacatgt gcaggtgcgg gtccacaaag gattcagtca cgaggttgcc
     2881 cccggcattg atggttttct tcccgcgtcc ctgaatcttc tttgaaatct ttgtgatgat
     2941 cccgttttcg atcccgatgt cccacgtgcc cggtttgcgg cgcagctggg cattcctgat
     3001 gataatgtcg tatgccataa ctttcagacc tcctgagggt tgttatttct tcttgttctt
     3061 cttgagcagt ttctgggcca gaagggctcc ttcccaaatc gcctcgtagc cggtgcagcg
     3121 gcacagatgc ccttccagca cgtcttcgat ctcgtcctct gatgcattca gattctttgt
     3181 gaaaagcgcg tacaggctca tcacaatgcc cggtgtgcag tatccgcact gcacggcgcc
     3241 ggcttcaatc agggcctgct ggatcggatg aacttccgtg ccgctggcca gcccctcaat
     3301 ggtaacaatc tccgcgccgt cgcaccgctt ggccgggagc gagcagctct tcttggcctc
     3361 gccgttcaca accaccgtgc aggcgccgca tgcgacatcg tcgcatcctc gctttgcgcc
     3421 ggtcagatcc agcttgtcgc ggagcacatc cagcaggcgc tcccagccgt cgatctcgac
     3481 ggagcgcttt gttccattga ctgtgagtgt aattttttct gacatggctt acttccttgc
     3541 tttgagcgct tctttcatct tctcgggggt gaccggaatt tgcgtgaact cgatgcccgt
     3601 ggcatcatag atggcgttca gaatcgaagg cgccacgccg atcaccgggt gctcgccgat
     3661 gccgcgcgcc ccgaacggcc cgatggtctc cggggtttcc acaaactcaa tggtctgctt
     3721 tttcggccga ttcttgatgg tcgggaagcg gtacttcttc atgaacggat tcagcatctt
     3781 gccgtccttg tcgaacttca gttcctcgtg aagcgtcgcg ccaagcgcca tcatgacgcc
     3841 gccggccacc tgcccgcgaa tctgccgggc attgatcacg cggccgacat cgaacgccga
     3901 ggcgaagtgg tccaccagga cctctccggt ttccttgtct atgcggattt cgcagccctg
     3961 cgccccgaac gtgtggctga cgcccatcgt gccctgcccg ttttcatccg ggttgctgta
     4021 gcgcggcagg cgggcggcat cggaggactg caccacttcg ccgacggtca tgccgttggg
     4081 atacatatat ccgtagacca gtccctccag cggaacattc acggtcggat cgttcttcag
     4141 ataaacgcac ttcccgttgt aatccagcat gtcgagatcc acgcgaagtg cctgcgcggc
     4201 ggtctttttc atcatcttga tcgccttctc gcagcagcgc acgacggcgc ggccgccctg
     4261 cgtggtgaac atggagccga tggtctgcca ctcccacgga gagaactgcg tgtcgatctc
     4321 ggtatagacc ttgatgcgct ccggcggaat catcaggcat tccgcggcaa cctgctggac
     4381 gaccgtgcgg agcccctgcc ccacctcggc gccgcccatg ttgatgcaca cgcttccgtc
     4441 ctggttgaac ttcaggtagc agcccttggc ggaattcggc gcgcccttcg gcgttttcat
     4501 cagcgcggcg aaaccgcggc catagaaata cttgtcgtct tccttcttct cctgcttctt
     4561 cccgtagatg gcttttttta caatatccac gcacttggtc acatcgccat tcacttcgca
     4621 cagcgtttca cccacagccg ttttcttgcc ggggccaaga tagttctttg cgcgcaactc
     4681 gaacggatcc atcttcagtt tgcgggccag catgtccatc atgcgttcgg tcgcgaactg
     4741 ggactcctga tgaccgtagc cgcggtaggc gccgacggcg ggctggttgg tgtacatcag
     4801 ataggcctgc agatcgcagt tcggaatctc gtatgcgccg gccgagttgt gcccggcggc
     4861 aatgcagaca tggatgcccg tatccgcata ggcgccggtg ctgtgataca cgcgcgtctc
     4921 catcgcggtc agcctaccgt ccttcttcgc gccgattttc atccgtgtac ggatgccgtg
     4981 ccccagcagc gtgctggtga aatcctcgcg gcggctggcc acccatttca ccggacggcc
     5041 cggcacaaag ctcgcgatgt aggcgacggt ctgctccacc gtgatgtcgg acttatagcc
     5101 gaagcagccg ccgagttcgg gaatgtgcac gcgcacatcg gaggacggcc gcccgtacac
     5161 gcgcgccagc tcctcgcgaa tgatgaacgg gcagatcgag gacgaccagc attcaatcgt
     5221 cttgtcctcg tgaaaccatg caatcgcgcc atgcggctcg atcgcgcagc ttgagccgtg
     5281 aggatagttg aactcggctt cgaccaccac atcggcgtcc ttgaagccct tctccgcgtc
     5341 acccttcttc agcttgtaga ggttggcgat gttggtgccc tgaatggggc cgagaccggg
     5401 cagatgccag tagtcgccgc tgttttcatg gaccaggacc gcatccttct caagagcctg
     5461 cagcgcatcc gtgagcaccg gcaaaggctc gtactccacc ttgatttcct tgagggcctg
     5521 cttcgcctga tagggatttt cggcaatcac ggcggccagc ggctcgccga tatagcgcac
     5581 cttgtccacg gccatcggaa ccaggtcgcg gatgttgtcg ccgtagagga ttttgcagcc
     5641 cctgcccgtc accaccttga caacgccctc gcatttttcc gccgccgagg tgtcaatgga
     5701 aaggatcttc gcatgcgcat aatccggacg gagaatcgcc gcgtgcagca tgccgggcag
     5761 cttgatgtca ttcaggaaaa tggccttgcc ggtaatcttc ttcaccgcat cgggccgcgt
     5821 gatgcttttg ccgacgtatt tgaattccat ggatcttcag tcccttctgt ttttaacgct
     5881 gaacaacggt tggtaaatgt aaaggttcca gcgggaaaat ccagccgtta tttggactgc
     5941 ggaccaatta atttcgcagg ttaatgattg cggaacaggg ccgggcgcac tacactgccc
     6001 catcaaaaag cagtaacctt tcaggagccg aaccgtgaaa aacaaaatat cctccgaatt
     6061 catccgcgcg ataccaaaga ccgacctgca cgtccatctg gacggatcgc ttcgcctgag
     6121 caccctcatc gagctggcgc gggccgagaa ggtggaactg ccctcctacg atgaagaagg
     6181 cctgcgcgag ctggtcttca agaacgccta taaagacctg gtggaatacc tcaagggctt
     6241 cgcctacacc tgcgccgtac tgcgaacggc cgaaaacctc gagcgcatcg gctacgaaat
     6301 ggtgcaggac aatattgccg aaaacgttcg ttatctcgag gtccgcttcg ccccgcagct
     6361 tcacatccac gaaaagctct cccttgagga aatcgtcacc gctgtctgcc ggggcatgag
     6421 ccgcgcgcgc gacgagcaca accgctcgaa ggccgtgaaa gacggcacgg acattccgtt
     6481 cttcttcggc gtgatcgtct gcgccatgcg ctgcttcaac gagcactact cgccttacta
     6541 tgcgcagctg tgcagcgcga tgaaatacgc gccggaaaag gaaatttttg caaccgcttc
     6601 cctcgaactg gcgcgcgccg cggaaatcct cgcgcacgac agagggctgc cggtggtcgg
     6661 tttcgacctc gccggtgccg agtccggcta tccggccatc agccacgcgg aagcctacaa
     6721 gtatgttcac agtcacttca tccgcaaaac cgttcacgcc ggcgaggcct acgggccgga
     6781 gtccatattc caggccatca ccgaatgcca cgccaaccgc attggccacg gcacatggct
     6841 gttcgcgcac gaccggatca gcaatcccga catcgccgat cccgaggcgt ttgttcacta
     6901 tctggtggaa tacatcgcca gccagcgcat ttccatcgag gtctgtccca ccagcaacct
     6961 gcagaccatg ccggaactga aaaacgacat ccgcaaccat ccgctgaaaa aaatgctgga
     7021 gcatgagctt tcggtgacca tctgcacgga caaccgcctg gtttccagca ccacgacctc
     7081 agacgagctg atgctggtga ccgaggcgct ggatctcccg ctgcgcgatg tgcgcaatct
     7141 ggtgatcgcc ggcttcaagg gcagcttctt cccgggaacc tatcccgaga agcgcgccta
     7201 tgtccgcaag gtgatcaatc agctggaccg gctggagcag gaaatgctgg gctgaagcag
     7261 ggtcagaatt cattgcggct gcgcaggaac gcgaagatgg cactgttcca ggcgcgccgg
     7321 tcgccgcgaa ccgatgtgag cgcaatccca atcagccatc tgccggatat cgagtccggc
     7381 gcaaacctga tcactcgccc gtacagacga tagcgctgac gctcctgcgg accggaaaac
     7441 agccggacat caagtttaac cttccggcct tccggcaggg gtgaaggcgc cgacacctga
     7501 agcccgcgcg ctgaaacatc cacaatgttt ccatccatct ttcttctgaa aaaagccctg
     7561 cgccagacca tccggacggc gccggtcttg attatcctga cattctgcct gtcttcagaa
     7621 gggccattca tatcgacctc cccgcgatct attcaaacac ttcggagagc tgatttcaag
     7681 gattgttcgc gcgcgaaccg gcaggagtcc gcaaagcctc ataaataaca ttctgccaga
     7741 tccctcgcag gttccgcgaa cggacccgaa gccgcacacc cgcaagagaa tatccgtccg
     7801 gaccggccgc ctttgaccat atgatcctgc ccgggaaccg gtagatggcc gcctcgctgc
     7861 caggcacccc caaggcaaga agaaccttca cacgccggcc cgccgcaacc ggcgccggga
     7921 gctgcacctg caggccgtcc acggaaacat ccaccaggct gccggaaagt attctgtgtc
     7981 cgaaaaaccc gcgccgcagg gtaagggtca ccgaacccgc ggtgcggatg cgcgcctgac
     8041 ggcgacgatc ggaggcggga ttcatggatt caccacatgc tgcggtttgc ccgccatgaa
     8101 ggcctcaatg ttccgcacgg caatatcata gagccggatg cgggccgccc ttgtggccca
     8161 cgcaatgtgc ggcgtgatga aacagttcgg gcagttcagc agcggattgt ccttcggcgg
     8221 cggctcaacg gacagcacgt ccagccccgc accggccagc tttcccgccc gcagcgcggc
     8281 ggccagcgcc gcttcgtcca caagcggtcc gcggccggta ttgatcagaa acgccgtcgg
     8341 cttcatcagg gacagcgttt gttcgttgat gaaacccctg ttcgcatcgg tcagaggaca
     8401 gtggagcgaa accacatcgc tttttgaaag aacagtgttc aaatcagccg gttcgactcc
     8461 ggacatggtt ctcggagacg gattctgcgc gaggaccttc atcccgaatg ccagcgcaag
     8521 ccttgcgacc gcctgcccga tccggcccag gccgatgatg cccagcgtca ggtcctgcag
     8581 ttcaatcagc ggaaaattcc agtagcagaa atcctccgat gccgaccagt cgcccgcgtg
     8641 cacaccgcgg ctgtgatcgg ccacgcgctg cgtcaggttg agaatgtgcg caaagaccat
     8701 ctgcgccacg gaggccgtcc cgtagtcggg gatgtttgtc acgatgatct ttctttcgcg
     8761 cgcggcgtcg acatccacca cgttgtagcc ggtggccagc acgccgatgt atttcagggc
     8821 cggaagtttc cgtatcgcat cgccggacag gaccgttttg ttggtgagaa tcacgtccgc
     8881 gccggcggag cgctcaggga ccatttccgg cggtgtccgg tcgtggatat gacactcgcc
     8941 cagcgcatgg agccgtttcc agtccagatc gccgggattc agggtgaagc cgtcaagaac
     9001 aacaatcttc attccgcgcc tactttacaa agtatcccga cacacgccat tccccgtcgt
     9061 cctccttcat gggggtgacg gtttcaaccg cgtttttctt tcgggcgaag gaggttttga
     9121 actggatcac aatatagtcg ccgtccggaa cgcccggcag gctggtaaaa tgccgggcgg
     9181 acttgaagtc gcgcgaaatg atttccccga gcggggcgcg cccggaggtc atcatgccgg
     9241 cccagtcgct ctccgcgacc gcgccctgga aataggcggc ggcgtttgac cagctcgcgg
     9301 cataggctcc gctgtccact tcctgcagcc atgcctgggc ggcgtacatg gcggcggcct
     9361 cttccgggtg gcgcggccgc gcgcagccgg ccagcagaat cagcgcaagt gtcaccatca
     9421 tatttttcat cgcaggcctc tgtgcttaaa agttccaatg tctggaagtt tcttcgcaaa
     9481 aagttccaat gattggaagt tttttccaga aaagttccaa tgattggaac ttttcatgcg
     9541 gtgaaccaga cttccgcatt cgcgcattcg cggaccgccg cgccggcggg gatggtttcg
     9601 ggcgccgtgc gaagaacctc cagcatgacg gccttgtccg cgcccgcgac aagaaagacg
     9661 atctgaccaa tgttccgcat catcgcgggc gtaacggaca cgcggtcgaa gtcgccaatg
     9721 cgctccaccg ggattgcaaa atatccggtt tcactgcggg cgtcttccag cgtgaacagg
     9781 gaagccgtgt gcccgtccga cccaagcccc agcaggccga acggaaactt cccgcccgcg
     9841 cggtggaaat cttcgaggcg cgcgccgtat tcggcggcgg ccatcaccag cggatgcgaa
     9901 gtgttcacgc ggataaaacg gtcttccgcg gctccggctt tcaccagcag cggggcgatg
     9961 gccccctggt tgctgtccgg cgagtccgga ggcaccatgc gctcatcgct caggaagaag
    10021 tgcacatcgg gactcacgcg gattttgcgg gaggccagat cccgatagac cggcagcggg
    10081 gttctgccgc cggacagcat gatcgccgac gggccccggg ccgactcgat cacctgctgc
    10141 agtttctggc ggaccctgcg cgcaaggctt ttttcggttt tgaattcacg aaaggtcagc
    10201 atgattcacc agcgcggggg aaattttgaa agcatcttct gaatgccggc ggggccggcc
    10261 gagccgaagg cataaggctc gggctcaatc cgtttttcgt ctatttcatg cagcacgggc
    10321 gtgaacacat cccaggaagc ctccagctca tcctcgcaga tgaacaggcc cgggtcgccc
    10381 ctgatcacgt cgagaatcaa actctcatag gcctccggga tcagcccgtc aaacgccgag
    10441 gcatagcgca ggttgagttc cgtatgctcc aggttcagcc ccaggcccgg aaccttgttc
    10501 atgatgccga atttgatgga ctcgtcaggc tgcacgcgga tgaccagttc gttgggaggc
    10561 aggccctccg gccatttgtc aaggaacaca tgaccggccg tgcgcttgaa ctgaattcgt
    10621 atttccgtca tgttccggtc cagcgccttg ccggccgaca ggatgaacgg cacgccggac
    10681 cagcggcggc tgcgcacgcg aagaaccgcc atcgcatagg tggacgtgat tgaatccgcg
    10741 ggcacacccg gctcttcgcg atagcccttc tggccgccgc cggcggcgta ctggcccagc
    10801 accagatcct ctatcgtcac cgggaggatg gaccgcagca ccttgacctt ctcgtcgcgc
    10861 acatatttgg tcaggctgac cggctgttcc atggccacca gcgcaagcat ctgcagcaga
    10921 tggttctgca tcacgtcgcg aatgacgcca atgccgtcga aatagccggc acggccctcg
    10981 atgccgatgg tttcggccca ggagatgaac actttgtcca cataaaggcg gttccagatc
    11041 ggctcgaaaa tcaggttggc aaagcggagg gccatcaggt tctggatgac ctccttgccg
    11101 agataatggt ctatgcggaa gatctggcgc tcgtcaaaaa ccgttttgat ctgctgcgac
    11161 agttcgtccg atgaggcgcg gtccgtgccg aacggttttt cgatgaccac ccgcgtccac
    11221 ggctggcttc ccgcgggccg gaccaggccg gcgccgccga gggcatgcgc gatgtcgtgg
    11281 aaaatggcgg ggggcacggc aaaatagaaa atgcggcagg cctcctgcgg gccctccagt
    11341 tcccgcagcg ttccgtaaag agcctgaaag gattcgcggg aatcataccg cccggagcgg
    11401 tagaagcagc gggccagaaa ttccgtcatg cgctcgccgc aggcctcgcc gggcgtatag
    11461 cggcaggtga gatgcgcggt gatcatttcg cggaaggctt cgtctgtcat cttcgtgcgc
    11521 gcaaaaccca gcacctgaaa attcgcaggc agcagattct ggcagtacag cgcgaaaagc
    11581 gccggtatca ccttgcggcg ggcgagatcg ccagatgcgc caagcacgat gaaactgaga
    11641 tttccattct gtgcattcat gatgcgtgca tgatgatcta ttcagggtcc gttggcaagc
    11701 tccccgacac gggcgggaaa tccagatgaa ggtccatccg ttcgatgctc tcgttcagga
    11761 cggccttaag gttattgacc cacagaccat aatcccgccc ggcatgatat cgccatctgc
    11821 ggtaatccca gtccgcatag tcccgcagat tttccgcgac agcgtcaatt cgggcgttca
    11881 gtgactccgg cgtcagcccg ctctcccgga gctcccgcca gcgcgcggac agcagtccgc
    11941 gatagcgggg cgactcattc tcgagccgct gcatcagtcc gctggcaagc cattcccact
    12001 ggccgggggc ggcccccagc tcaaaatccc acgggacatg gaagaagaac ggccggtcgc
    12061 gttcgcagac aaggacttca tgcagaagga aatcgaaggg atatccgttc cggttctgaa
    12121 acaggttcag aaggagctga taatccgcaa gattgggaat gtccattgtg cgcgccagct
    12181 ccggttccca actctcgctg cccggctgtt caagaaaccg gcagaaactg cggtaggggc
    12241 cggagaattc gccctcatgc ccggcaggcc ggcgcgcgcg catgtccgga atccggggat
    12301 gaacggtttc gtgccggtaa atgatccact tgtgcgcatc cgccggcggc gagccgccgt
    12361 tcagcagtgt ttcatccacg cgggaggcca gttcataaag accctgataa cggccgttca
    12421 cgaaaacctc ggcatgacgg acctgcggcg cgaagcgcgg cgcaccttca ccggcccagg
    12481 accggaagag atcatacgcc aggccgttgc ggacaaaaga cggatcctgt tgcgagttca
    12541 ccgccagcag gacacgactc ccggattcat ccagaaaacc gtgcggttcg tccatcttca
    12601 gggaaaaaag cttcttcgga ttccagtagc tggaattccc gcgatgctcc attccgccgc
    12661 gcgtaccctg agttgcgtac cggattcgcg acgggaaatc ctcaccggca ccgcagaatt
    12721 ccaccgccgc atcggcgcgg cgtgatttat ccaattcatg acggacattc aggaagatcg
    12781 cgggaagcgc aatgcggcgc ggcatgacac ggaatgtcag aactttctcc acagattcgg
    12841 ttccgtcctc tacggcggcc gtgagcacca cccggacggg accgcccgta aagttccgcg
    12901 cgacatggcc ggcatggtcc agaacatcag gattggaact gctccagcgg atttcaacgc
    12961 ccaggtttct gagcgcggcg aggtccagct gaagcggagc cacgatgcgg tcaggtgaag
    13021 cattgcttcc cagcaccatg aactcatcga ggaagccggc cgccctgcgg gcgggtgaca
    13081 gatgctccgg ggcgggctgc cagatcgact cgatctgctg ccggccccgg gccagacgcc
    13141 agctgatttc ctttcgggaa aggggattct gcagatcgcc ccgcaggcat ccgccatatt
    13201 ccccgacaac ttcatcgtac agcaacttca tctccgccga ggaaagcggc cacgcggcgc
    13261 gggaagccga ggactcccag gacggccgga gggcctcatg aaacgggagc caccactgcg
    13321 agggatgacg cacggcaccc atgcagacat ccgggagatc acccggaaga agccccagcc
    13381 ggtcatgaac agaccaggca taaacccccc gggaaagccc gttgatgcgc aggcggcaca
    13441 ggccgccgga tatggaaggc aagcccagcc gatgccggag gcgggcatcg aaaacgggaa
    13501 aaagccagtg accgttttcg ggcggactga gcagaaggcg gcgggcgccc agaacctgcg
    13561 ccccttcgag cagctgcagt tcgtagcccg gacgtttccc cgaatggtat tcccgatcgc
    13621 ttccggagag acaaatcagc gcgggataga cttctccccg aaacgccaca tgcacctgcc
    13681 gcggccgggc cccggcctgc gtgcgccggc ccgatttcaa actccgatgg tgagccgcga
    13741 tcagctcacg gcgaaccttc ccgggcagaa tcagccggag ctcatcaagg cggctgatgg
    13801 cccgcgcttc gctgcggccc cggcgggtca aaggcgcaaa cacatcgggc agggccgctg
    13861 aaacacggag tatccgcgat cccgtctgcg ccgcgcgcca tttgaaacag cggcggccgc
    13921 tccttcccgt ccacagcagc ccccgtttcg cacgggcgag gccgcggatt tcctgttcag
    13981 aaagagcgcg gttccatacg gaggcctcat ccagccatcc gcgaaacgaa tttcgcgccg
    14041 cataccacac gggtttgccg aaggccatgt tttcgcgcgg catgccatgg aacacgaaag
    14101 gctcccgggc catcggggag ccgttctcgt acagaatgat ttctccccgc tccgcatcca
    14161 ccgtcgcggc cagatgcaca aattctccat atcgcacaaa cggataggat gcgcgctgcc
    14221 ggccgcccgg agtcggcatg tcaaatgtca tccggcccgc ttccagatgg aatcccactt
    14281 gaacgttttt ccccgcgcga taccagatgc actgatcggg ccggccttcc ggcatgcgaa
    14341 tccagaaaga cagcgtgaac gaagagccga tgcggtccca tctcaggcgc gactccgcat
    14401 atgttctttc ccggccgtcg aacttgcgcg ccgcgccgaa gcggccgggc gccgacgtcg
    14461 tgccgggaca caggagcgga tgacctgtaa tgaaatcgca gggcgcgggc tcatcgaaag
    14521 ttatcagatg cagaagcccg ctgcgtgaag ccagcgcgcg agagagacgc tcgcgcagcc
    14581 gctgctcctc accgggccgc ccctcaaaga tccattccgg ggaaacaaaa acggccaggg
    14641 ccagatatgc cgccgcgact gcggcggcgg cccatcggac ggccctgacc ggtgcggacg
    14701 gcatctcagc ggattccgcc ggggcgatcc agaaacacgt gaatggaatc cgccgcgaag
    14761 gactgtgtga tttcctcctg caggcgcacg gcctcggtct tcaagccgga gaaagtcacc
    14821 tgcacactga ctttcccgtc acgaaccccc acgctctgaa cggaatgatg ccggaaatgg
    14881 gatttgagca gttttgaaag ttcgtcagac gaaaaggaac cgcccgcggc ggctgtcacg
    14941 acaagcagtc cgtcgcgctt cagaaacccg agagtccgga accagcgggc cagcaccagg
    15001 ctggccgcgg ccagaacata cagtaccgcc aggaaaccga aattgtaggt ggcggcggcg
    15061 acggatgacg cgatcacaag catgatgaag ccgacttcct ccggctcctt gatcggggtg
    15121 cggaagcgga tgatggagag agagcccagc agtcccagcg acagcggaat ggaaacctgg
    15181 atgcagatga aaagcgttgt gacggccagc cccagcagag gaaacgatcg cgccacctgg
    15241 ctgccggttc cccggctttc atagaagcac cggtacagcc ccatggccag aaacgaggcc
    15301 agaagcgcca cgagcagggc gccggcaaaa acggcgaggt tcaagttttc ggaaatctgc
    15361 tcatacgcgg acagcgcgtc aatcaggcgg atctgcttca taatctaaat gctcccttcc
    15421 atacgcattt ccataaaaag tccatacttc gaaaaactct gcatgcggaa acccagacgc
    15481 atcagggtct ctccccatgg ccattcgcgg gccgcagggg ccttggcctc gcagacaatg
    15541 cgttccgacc ccagcggggc ctctcccgaa aacaggtctg tgtttgcccg cagcgagcag
    15601 atgtccgaat cgagattgat cctggcgccg gtccacggac acacatagcg gcggcggcgg
    15661 taccggatgc cgatggtcgg cacgagtgca tcggtaaaag gcaggcctgc cgccatggcc
    15721 gcaccgcgca gcaggtcccg gagagccgga tcacggagcc gcgcgcggca aagcagttct
    15781 ttgtttgcgc ggaaggcgac gtgccgtttt tcccgcaggg cgcccatgcg gtccttcaca
    15841 tccagaaaag ccgggatctc tccgcagccc gtcggcggaa tgccttcggc ataccagcgc
    15901 aggcgcacct tgcgcttgta cgagtccccg ttgacctttt cccagtagct ggagaaaaaa
    15961 tcatcgtcaa aataaatgct ttcgacaatt ccctccggat gctccggatc aggaaggcaa
    16021 tgaaggtcca gcgcggcctc ggccgccgca agggcttccg ccggcgccac atatttcagc
    16081 tcgcgcaccc gcgccccgcg cgattcctcg cttaactgga acattccgat cgtcctcacc
    16141 tgcccgcgca tcgcttttca gagagaccgg tcacacgcca tctgaaccgt gcctgaacgg
    16201 gcctcttgat cgcggacagg ctgccagagc agacggcgcc tgtcaagcag ggcgctcact
    16261 tcgcgcgtgc gggaggatcc tcctcgttga gcatccagtc gaatttgagc caggagatcg
    16321 aataatcgcc cgtcagcagg aattcacggg tggcgccgtt gacgtgcgac gcaatgaatc
    16381 ccagcatggc gcggtcggcc ggtgaaaaat ctccggccgt gatgatgtcg gagtagcggc
    16441 tgatgcaatc cccgcacagc tccttgaaaa caggcgaaag ccggattctt tttttcccga
    16501 cgctgacctg gaagttccgc aggtcgcgca gaaagttttc ggtctggtca cgaagctgtt
    16561 cgggaagttt ctccgccgta taggcctcgg gccggagcgg cgggcttccc ttcgcgccct
    16621 tgaccagcgc catgtggacg ccgggttcat cgtaatagcg ccgaagctcc tcttccagcg
    16681 agtcaagggt ccggtcgcgg cccagcaggc ggacggggat ttcgtcgaag aatccatcat
    16741 agcggcggac gcttaatccc ttctggaacc atccgttcgt cctgaacagc acatgccgct
    16801 gccgggaaat gatctgcagc acgcgggcgt tgtaggcatt gatcaggaac gcgagccgct
    16861 cggcctcggg ccacgcggcg aactcgtcga gggatatttt ctgcatggcc tcgataactt
    16921 cgtccagcgc cgactggccg gcctgaagtc cccagtagtt caccatgccg cccggtttaa
    16981 cggttcggct gagaaggcgt gagtactcgc tgaaatcaaa agacccggcc gatgccgcgg
    17041 acgccgccag cagaatgccg aggaacagcc ccgccgattt catgcccgcc tccttttcag
    17101 aaggaacagc tcgtctgccc ctttttcata agatactgct gatggtattc ctcggcgagc
    17161 caaaaatcag aggccggtga aatttcggtg acgatctttt tcctgtttgc ttgctgctgc
    17221 ttttcgagcg aggcccgcgc agcctccatt tgtccgggcg agtgacagaa aatcacgctt
    17281 cggtactgcg ttccgcggtc cgggccctgc cggttgagct gtgtggggtc gtgcatggcc
    17341 cagaaggcgg cgagcagttc ttcataactg actttcgcgg gatcgaaccg gacctggacc
    17401 acctcggcat ggccggttct gtcggtgcag acctgttcat aggtggggtt cttcaccgtg
    17461 ccgcccatgt agcccacggc ggtggaaacc acgcccggaa ggcggctgaa gagggcctgc
    17521 ggcttccaga agcagccgga cccgaaagtc gccgtctcaa gggtccggct gtcggcgaac
    17581 aaataggcga attcgccata cccctgcgcc tccagttcgg ccgcgggaac aaaccggagt
    17641 gacgccgaat tgatgcagta gcgcattccg ccggaatctt ccggcccgtc gtcaaacaca
    17701 tgacccagat gcgaattggc cgaagaggac cggacttcgg tccgcttcat gcccagtttg
    17761 aaatccgatt tttcaacaac gccctccggt ttgaggggtt tggtgaaact gggccagccg
    17821 gtgccggaat cgaacttgtc gcgcgagctg aagagcggct cgcccgagac cacatccaca
    17881 tagatgccgt cctcgtgatg atcccagtag gcattctgaa acgggcgctc ggtgccgtcc
    17941 tgctgcgtga cacggtactg ttcgggggtc agcattttct tcaattcggc atcggttttc
    18001 ggattgtcgc gcttcatggg tttggatcct ccgccggggt cgcccgtcag aatcacaagc
    18061 ccggccagga aggccagggc ggccgccgcc attgttcttg cgaatattct gaccgtcttc
    18121 atgttctctt tactccttta aatatccccc atggagcggg aatattcaaa cgggtggaga
    18181 gaaccgtcta ttcttccatt tccccgcccc accagtaggc gctgagcagc gcggtgaggc
    18241 cggccacaaa cgcggcgtga tccgtgttgt cggggaaggt cagggaacag gcgtccgcgc
    18301 aggtgatgta atcgctgtac agcgaacgga ggtccctgat ctcgcggtcg ggcgagccct
    18361 cgccccaggt cagtatggca tgaatgacct gattgcggta tttgcgcagc accatggcca
    18421 gcgtgcgggt ggcccggtgc gacttgggcc ggcgcggcga aggatagcgc gcattcgaaa
    18481 tcagccgttc caggtcggtg gcaatggttc cggccgtaca tgcggaaccc cggttgtgaa
    18541 tccgcatcca cggctccgtg atcctccaga ccggcttgct gccgtccttg cggcgaatca
    18601 ggatggaaaa atactgcgac ggctcccgcc ggtcgtccga ctcgcaccat gcctcccgca
    18661 gcaggcgggc atgaccccag cggaagaaag gcttgtcggc cttgggatca tagatcacaa
    18721 actgagactt gcgctccaga taaccgacca cggccaccca gtgttcaaaa tcgttgacga
    18781 gaagaatggc tggaccggcc ccggtcaggt ggccaagcac attccgaagg aagacatgcc
    18841 cctcatcgcg gcacgggatc agaatctgct cggtcatggc cgcgtacttt ctggcggcat
    18901 gggccagctc tttttcatca aggccttcct tgaaaacctt gtacggcttt ccggccgccc
    18961 aggccacatc gcgctgcgtg gcctcaatgc ccagcataaa aaggcagctt gccagactgc
    19021 atggtccgca atgaccaggc tcctgcatca cggcaaacgg actgaactct tcactcatcg
    19081 gaaacagctc cttctttccc tttcatggaa agaaattcaa acggccatgc attttgcggc
    19141 attggccgga caaaaagcaa atgcaaaaga aaaaaaccgg aattcacgcc cggaaaaggt
    19201 cttttttgct tgacttaact catttttaat caaattgtta caaaaaaagt ctccactgat
    19261 ccagtttgac cccaaaacgg ccgaaaacgc tcaaaattcg tcgttttttg tgatttttgg
    19321 gcattttttg acgatttctc ggcatttttg gcattattgg ggctgatttc gagcctggaa
    19381 aacggatttt tcctgtttat tatcagttta tcatcaattg tttgccattt tttcagagcc
    19441 ctcatgcctt gacatagccg tctcatttcc agatgctggg ttttggatat gaaatcgagt
    19501 gaaaataacg gcacgcggcg gggggcatcc ggccggctcg cggcggacga gcatccgcat
    19561 gcgcccctgc tcccttcccg gcctcgccgg gaaagcaggc acgggaaaac ccgtgccgac
    19621 ccctacgcat gggcggccaa tgccggcgat ccggaactgc ggaattttct gcgcgcttcc
    19681 aacctgcacg cgcggcagag tctgcgcccc ttcagcggtc tgaaacgcca gattgaatcc
    19741 gagcttcgcg cgctcacgcc gacgctggag ggcggcgctc ccttccgctt cggccggcat
    19801 gtatatcatt tcatgcatcc cggcgatcgc gattaccccg tcctgctccg gtggcccctt
    19861 catgcgccga accgcactga aacagtcctc gacatcaatg tcctggcccg gggccgcaga
    19921 ttcctcgccg ttccccactt tgcgctttca cccgatgaac gcctccttgc ctatgccgcc
    19981 gacacgaccg gaaacaggcg ctatcggatc catgtgcgcg acctcggcac ggggcgtgat
    20041 ttgccggaca tcatccgcaa tgccgactgc ttttttgcct ggagccgcga cgggaaaacg
    20101 ctggtgtact ccgcgcttga tcccgtaaac cagcggccgg ccgccgttcg tctccgcaca
    20161 ctcggaaaag cggcgcgaac ggacaaaacc ctctacaccg aatcggatga ggaatttcgc
    20221 tgcattctga tgccgaccac atcgggtcag tatatccgca ttgtgtgctt ctccgcggac
    20281 accgaacaaa cccttctcct gaaggccgac cgcccccttg aaaagcctgt tgccgcggcc
    20341 cggcggactc cccgatgcgt ggacgatgtg gatcacggcg gcgggctctt tgtgatcagg
    20401 accaaccgcc ggcgatcgga attttcactg ttcacagcca ctcttcccga cctggccaaa
    20461 ggccgctgga gggcgcttgc ccctccgcgc cagggaacct ttgtggagcg aatgagcgct
    20521 ttccgcgaac atgctgttgt gaccgaacgc cagtcggcct gccagcggct gcgcatcatt
    20581 tcgctgaaga ccagggcttt gcggacacta gcctttcccg agcgggtcgg cgaggcctgg
    20641 gtcgagccgg tacccggcat gagtggctcc gtcgcacggc tcggctatac ctcgctggtg
    20701 actccgccca cgttctatga ctgcgcactc aggacaggaa aactgcgccg cgtgcagcag
    20761 gagctgactc cgggttatga gcccgccggt tacgaaacct ggcgcgagtg ggccacagcg
    20821 cgcgacggca cgcgcattcc cctgacgctg gccgggcggc gcgaaaaccg ccgcagcggg
    20881 aaccggcccg tgctgatctt cgcctatggc gcctacggca cgaacattcc gccctggttc
    20941 aatcaggcgc gcctgccact gctgaaccgc ggctttctct atgtgatggc ccatgtccgc
    21001 ggcggatcgg aacttggaga agcctggcat gccgcggcca tgcgccggcg caagggcgct
    21061 gccgcccgcg acctgaatga ctgcgcccgg catctgatca gaacgggccg cgcggaccgg
    21121 aaccggatct ttgctgaaac cctgagcgca ggcggcatca cgcttggcgg agcaatcaac
    21181 gagcagccag ggctcttccg gggcatcatc gcccatgcgc cttttgtgga cccggcaggc
    21241 agcctgtctg atcccgatat tccgctgacc ctgtcggaat atgctgaatg gggcaatccg
    21301 gcgcgggcgg ccgactggcg cgccatgtgc gcctattcgc cgtatgacaa cgtcacacag
    21361 tgccgccatc cggcgatgct ggtcacctgc gtgctgaaag attcgcaggt ccctttctgg
    21421 gagccgctga aatgggtggc ccgcatgcgc cgcgccgacc ggggcggcgg accaatactg
    21481 cttctcacgc agcaccacgc ggaccattcc gggccctccg gtcgcacacg catgatgcgc
    21541 gaacaggctt tcgagtgggc attcctgctt tctctttgat aaacgaacgc ggacagcagc
    21601 cgcgacaggg cgcggccctc caggatgttt cggcagacga atttcacggc gttttctgct
    21661 tcagcgcatt tcccgggcca ggaggttctg gtagatttcc aggtcacgtt gcgaaaagca
    21721 gcagaagatg acttccatct ccgattgatt atcacgcaga aattcgccgc aggttttgac
    21781 ggcaatttca gcggcggcgg tcaccggata accgtagaca ccggtgctaa ttgccgggaa
    21841 cgcaatgctc ttcaggccgt gttcggcggc cagctccagc gaacggcgat aacagcccgc
    21901 gagtttatcc tgctctcctg aattcccgcc gcgccagacc ggcccgaccg cgtggatcac
    21961 atgcttcgcg ggcaggcggt atccgcgcgt gatttttgcg tcgccggtcg cgcagccgcc
    22021 cagtgtccgg cactcccgca gcagttccgg cccggcggcg cgatggatcg cgccgtccac
    22081 accgccgccg cccagcagac tggtgttggc cgcgttgaca atcgcatccg tttccagcgt
    22141 cgtgatatct gcgcgcacgg ctctcatcat tctttcctgt ttgaacagtt tttacaaaaa
    22201 gcgatccaat atcaagacgt tgatgcggac tttcgggatt gatggcttca aaatacccga
    22261 tcaggcttta tttttgcggg ggggcagtgt agattgcgcg aaatggttgt gcagcttgga
    22321 agaatagttc tgttcacggc ggtcctgacg ctggccggcg cgcagtccct gtttgcgctg
    22381 gcgccggtca cggacgacgt ggcggacctg tactcgcgcg ccgcgcgcat cgccgttcgc
    22441 cgcctgcccc agatgcacct gaaccagcag ccctgcaacg acttcgtggc caccaacgcg
    22501 cttgaaacct tcctgaccat gctggatttc gatcacagcc tttttctggc cgaggatgtg
    22561 agcgccttcc gcagggaggc gaaggatctg gacaaccgcc ttgcccgggg cgatctcgat
    22621 ttcgcattca aggtctatga tctcctcatg gtccgcctgt ccaaccgcgt ggagttcgtg
    22681 aaccggatgc tggacaaggg ctttgacctg accgtggacg aaacctatgc gtgggaccgc
    22741 cgccatgcgt catggccctc cggaaccaat gagtggaacg acctgtggcg ccggaaaatc
    22801 aagaacatct acgtgggacg cgtggccgcg cgcctgctcg gcgaagaaaa aaaggccgcc
    22861 gcggaacagg acggagaaga gccggacggg gatgaagcgc tcgacgagga gctcgatctg
    22921 agtccggagg atttcatccg caagcagttt cagcagcaga tcacggtctt caatgacaac
    22981 gacgcgcact ggctgctccc gctctatctc agctcgttca cgcgcatcta cgatccgcac
    23041 tcagactata tgtccgccaa tcatgccgag gatttcgaca ttcagatgaa gctctcgctg
    23101 gtcggcatcg gcgcgctgct ctccacggag gacggggcgg caaaggtcga acgactgatt
    23161 cccggcgggc cggccgaact ggacggccgg ctgaaggcgg gcgacaagat cattgccgtt
    23221 gcgcagggcg atgagccggc ggcggatgtg ctgcactggc ccctcagcaa gacagtccgc
    23281 ctgatccggg gagaaaaaga cacgaaagtg gtcctgaccg tgatcccgaa atcggacatc
    23341 tcgggagcaa ccacgcgcaa aatagacctg atccgcgcgg aagtgaaact ggaggacagc
    23401 gctgcgaagg ggtccgtgga aaacatcaac ggacttccgg tgggggtcat ccgcatcccg
    23461 gagttttatg cggacatccg cgggcagcgc cggggcgaca gcgaagcgcg caccgtcacc
    23521 cgggatgtgc gcgccattct ggacggcttc aggaccaatg acatccgcgg cctggtgctg
    23581 gatctgcgaa acaacggcgg cggaccgctt tacgaggcca tggagctttc gggtctcttc
    23641 attgaaaagg gccccgtggt gcaggtgaag agcagccagg gcatcagcat cctgcgcgac
    23701 cccgatcctg cgctggtcta cagcgatccg ctcatcgtgc tggtcaaccg ccagagcgcg
    23761 tcggcttccg aaatcgtggc ggccgccctc aaggattacg gccgggccgt gatcgtcggc
    23821 gacaccaaaa cacacggcaa gggcaccgtg cagacgctga ttgatcttca gcggtcgaag
    23881 cccgagctgg gctcgctcaa gctgaccacg gcaagtttct atcggatcgc gggcggctcg
    23941 acgcagatcg agggcgtgaa gaccgacatc gaaattccct ccatgctcga ttatctggaa
    24001 gtgggcgagg aatatcttcc gcacgcgctg ccctggtcgc tgatcaagcc cgcgtccttc
    24061 agccgctttg atcatctgca ggacattctt ccggaactgc agaagcgttc cgagaagcgc
    24121 cgcgcggagg atccccgcta cgccgcctac cgcgagctgc tgacctatct gggcgaaaag
    24181 cgcgccagca aggaattctc gctgaaactg gacgaccggc ttgcgatggc ccggcgcgaa
    24241 gatgaaatga gcgcctatct gaacgatgtc ttccgagagg atgacgagga tgaggacaaa
    24301 gatgcggaag atgatgaaga ccaggccgac ctgattctgg atgagaccct gctgatcctc
    24361 gcggacctga taaccctttc ggaagagaaa aggcaggtcc cggatgacgc ccagcctgcg
    24421 gctgaagtcg cgaaagaata agaaccccgc gggctgaccg cccaattgac cgctctgacc
    24481 tttcgctcct gccggacaag ccggcagggg gcgaagaata tccgcgcacc tatcggatcg
    24541 gggaattgac ggtcttgacg cggtaaaagc gctgcgggcc ggcatcaaga tcattcgtga
    24601 cagtaatcat tccatccaca ccgggcaaat caacagagcc cgtcaggttg gaccacgagg
    24661 cgccgtcatc catgctttca ctgacctgca gcgtgtaatc caccggaacc tccgacgtcc
    24721 acgcgcatac cacctccggc ccctgaatct gcatctcctc aatcgccgtc tccggcaccg
    24781 ggaaatggcg ttcgaaaacg caggctttcc cctcatatat ggtttcccat ggcgcaccaa
    24841 tcaacactat gtcaccattg agcgatacgg catgtccaaa cctgtcccaa tcatcagcat
    24901 ctgacgctgt taactgcgca accaatcccc acgcatctgg acccgctgcg ttgcgatgaa
    24961 acacatatgc tgaacctgaa tctaaaatac ctcccaaatc atcccactcg gctccgatca
    25021 gagccagata ttccgccact gacacggcat tgccgaaacc gtcaccagag gcgccttcag
    25081 gggccagtaa cttgacgacc tgcccccatg cgtttgtgcc tcccgtattg cgctcaaaaa
    25141 tgtatgcggc tccccggtaa gcgttgttgc ctgttgcgcc tatcaacgcc acagagccat
    25201 ccagcgatac tgaaaatcca aagtaattcg attgtgcccc atcttgaggg gtcagtttcg
    25261 tctcttgccc ccattcattt ggtcggcccg gccagcgtat cacagcggca tttctgaacg
    25321 caaaatcaaa aaaatggcat ttttggcact tctgtcccag tattaattcc acagcatcaa
    25381 ctggttaggc ggtgatagtg
//
LOCUS       DAOQWR010000020        24741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2349_length_24741_cov_5.686908, whole genome shotgun sequence.
ACCESSION   DAOQWR010000020 DAOQWR010000000
VERSION     DAOQWR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2349_length_24741_cov_5.686908"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..926)
                     /locus_tag="PKK36_02980"
     CDS             complement(87..926)
                     /locus_tag="PKK36_02980"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93554.1"
                     /translation="MADQKKKLDAETEVWNAISAFEQIIEAMPEDRLALETLQEAYEK
                     IGDQAQALNYLVRLAKVMLQEGDTAGAIGLQPKLTSLGGKDPAAREILETIQQLQGSA
                     KPPAANTPAAAETRGRAPAKRLDISNEMSLAWELAQAGEITQDEYTEVVHDLTENSTK
                     ALNSPVTVLHVLEDRQYKHRERILAYLAKSAGMPLITLTAFELQPAAFNLIPMDVMVR
                     QGALAFDQMAKDLLVAILNPFNKDLQENIKAVTGRECHFYLVSAADFETALEKIRKAS
                     AAA"
     gene            1118..2830
                     /locus_tag="PKK36_02985"
     CDS             1118..2830
                     /locus_tag="PKK36_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR93555.1"
                     /translation="MAAGKMSNKVVDILIKRHKLDPEGLDNLMQEASEAGARLEDWLV
                     KKKAVSQADMTLAVAEYLRMPPIELQNFVPDPKLLDLIPSKIMGQHLLVPMCRCHNML
                     TIALGDPFDVTAQDEVHALTGLDVFPVVAAEAQIKALIQKHTQDAATGLEDIIRDVTE
                     GDIELSKTEEEQVSLDEMLEEAEDAPVIRVVNSIMVEAIRRGASDIHIEPMERSIRLR
                     YRIDGELYESPSPPKPLQAAIISRLKIMSNLDIAERRIPQDGRFRIRALGKEVDIRLS
                     ILPCVHGEKIVMRTLDKTSLAPSLAALNLEKTAYENLMFGISQPHGLILVTGPTGSGK
                     TTTLYSALQELNTTDVNIITVEDPVEYQLAGINQVQTQASVGLTFAAALRSILRQDPD
                     IVLVGETRDGETAAIAIQAALTGHLVLTTLHTNDAAGAVSRLLYMGIEPFMLASSLIM
                     AQAQRLYRKLCPVCKQPIDIPTETLRLNHLDPAKFEGVQFFYHKGCPKCGNIGYKGRG
                     AVMEILMVNDHIRKLILDNADSAAVRTQAVSDGMRTLLDVGLDRVRDGLTSIEEALSV
                     AGGE"
     gene            2843..3955
                     /locus_tag="PKK36_02990"
     CDS             2843..3955
                     /locus_tag="PKK36_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR93556.1"
                     /translation="MAERTTGTGAHIGGKVKKIRIPGAKKIARIELPAFTRQLAAMLS
                     SGMPVVQTLSALEEQIENTNFKTVIGGVRVQIEGGSSFTDALRKYPDIFDDLYVSLMQ
                     AGEMGGLMAETTARIAAFLEARERLRRKVKSAMTYPVIVLTIVFGITAGLIIFVVPKF
                     AAIFSDFGAKLPGPTQFLVDLSEGVRKYALFVVLGIVAFVIAFGKFKRTPKGAYMLDA
                     LNLKLPVFGELTTKVALARFASTFAQLIRSGVPILQSLDIVAFATGNKVLGETIMKGR
                     DSVERGEPLSNALSADPKFPRMLVHMLSAGEKTGRMEDMLQKIAEFYDEEVEAMLDGM
                     TALIEPLMIVVMGVVVGGIVLCLFLPIFKMSEIVSM"
     gene            3974..5371
                     /gene="argH"
                     /locus_tag="PKK36_02995"
     CDS             3974..5371
                     /gene="argH"
                     /locus_tag="PKK36_02995"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNR93557.1"
                     /translation="MPNREANRKTTVGAVDAEVLAFTAGRDAVLDLALAEADCIGSAA
                     HVTMLSRMRVKPRLFSDQDRRAVIGELVQIMKSARRGRFQITVEDQDVHLAVERLLTA
                     KLGVLGKRIHTGRSRNDQVALDLRLYAKTRLQAAVARAAELARELLLFARRHQKVPMA
                     GRTHMQPAMPSSVGLWASAHAESLLEDIMLMINAYELNDQCPLGAAAGYGVPLPLDRQ
                     LVSDLLGFSRPCQNVLYAVTARGKLESIILSALSQAMLTLSRLSEDIVIYSMPEFGYF
                     RLPPAFCTGSSIMPQKFNPDVLELIRARAVRVASDAQAVAAVVRGLGSGYHRDVQETK
                     EPLMNGMDTTESALRIMTRFIPALKVDRAALKKGFVPGVFAADRALALVAGGMPFREA
                     YQHVKDHLDELSAADPDRALAAKISLGAPANIDFAALLERAKQASAWAADEQKSFNKT
                     ISRLLGQKYPLSPQR"
     gene            5376..6122
                     /locus_tag="PKK36_03000"
     CDS             5376..6122
                     /locus_tag="PKK36_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93558.1"
                     /translation="MAGTEYPIELDALAVSYPVHGGQVDVLRGISLKVGRGQIVGFLG
                     PNGAGKTTTIHVLLGFVEPAAGSARLFGEDVRRTIARQRIGYLPEHPQTYGFLTGREL
                     LRTAGRLFGMRGPSLENRVKELLGTVDLESAADRRISTYSRGMMQRIGVAQALINDPD
                     LVILDEPTGGLDPLGRMQIRAIIGDLRTRGKTVFFSSHELSEVERVCDSVVMLVGGRI
                     AAAGPVSELVQPSESLESYFLRVVERGLAS"
     gene            6119..6907
                     /locus_tag="PKK36_03005"
     CDS             6119..6907
                     /locus_tag="PKK36_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR93559.1"
                     /translation="MIRILTIAGAVWREMLRRRDFYVLLILLGALLFSLMSINVFGLG
                     STMRYMVDTGLLLAWIFSAVLAVTLSARQLPAEEQRGTIFALLAKPVTRWQLLAGKAL
                     GAWSAVSAATAAFYALLLAVLALKGWSVDLTVTLQALVLHVTALAMAVSLALAVSTRT
                     TFGAAASLTWLALAAGFVFVPQIPALLVYSDRVSGAAMLVLYYALPHFELFDLRQCVV
                     HDWPALPANILFPVLCYGLLVTAIFMLLAWLGYRGRHFKRGDVL"
     gene            6904..7785
                     /locus_tag="PKK36_03010"
     CDS             6904..7785
                     /locus_tag="PKK36_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93560.1"
                     /translation="MKDARSAALKTAWPWLLLLALWTAAFAAVCSLPQEAWAHAGEGA
                     SVTDRLLGSTRMIIGEAFYEQSDNYFHRGVPHQKKKAFHDIFQTWAHHVRPVSHEHAT
                     GKSVYEVMPWLDFTVRMNPHHVEAYLVAAYWLAGDGERPDLAMQIVADGLRANPGDYQ
                     LHAERGRLYLQQGENDRAEKALETAVRLWPSGYDPDDPMIRYDLARSLSYLSVLRQAA
                     GRLEAASESAARAAALFPQNTSLRARAGELARGTATMDAARRTIEALFPVQSKAGRHV
                     CSRGENHDHENEHELEH"
     gene            7799..8608
                     /locus_tag="PKK36_03015"
     CDS             7799..8608
                     /locus_tag="PKK36_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR93561.1"
                     /translation="MYLDYYQLREMPFNITPDPRFLYFTGRHREAFDHLLYGIEHRKG
                     FIQLTGEVGSGKTTLCRAVLSRLPTSIRTALVLNPCLSETQLLRAIINDFGLDPAGND
                     RLAYIEQLNEFLLGQLEEGYNVALIIDEAQDLEPAVMEQIRLLNNLETDQHKLMQIVL
                     VGQPELEEKLARNELRQLRQRITVKAHLSALSEEETADYISHRLEVAGAAGRIQFDGG
                     AIRQLHKIAGGVPRIVNTIADRALMAGYIVNTFTIGAKEVKRAIRDLELKP"
     gene            8605..9276
                     /locus_tag="PKK36_03020"
     CDS             8605..9276
                     /locus_tag="PKK36_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93562.1"
                     /translation="MSLIQEALRRKEADDKGVPPPPPVPSPAPKKDSPLKAVIAVLAV
                     LLVLIGVAVYLFTYLVNSFTPRRIAPPAPVQMPEAVKPAPVQVVEKTPEPEPPEVLES
                     EPVAAAAPVREIPEPAPEPVVTEAPRPEPVPEPVSVAAAEPVRLEVKTVAQPATVEWP
                     NLNLTGVLRKGGSGGPASAMISGELVSVSETIRGVRLVAVEASGVWLELNGEQKFLAI
                     GRSLK"
     gene            complement(9288..10526)
                     /locus_tag="PKK36_03025"
     CDS             complement(9288..10526)
                     /locus_tag="PKK36_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNR93563.1"
                     /translation="MSDIRPFCGYRPRADAASQIASPPYDVLNSDEARERVRGNPLSF
                     LHVTKSEVDLDPSIDVHSDVVYQRGADNLKKMVADGLLIKDREPCMYVYAQRMGEHVQ
                     TGLLCGGSVAEYQNDLIKKHELTRKDKEDDRTHHVEMLGAHTGCVFLTYRARPEIDAI
                     IGRICAQKPVYDFTADDGIGHTLWVVDAKADIAALQTEFKKVPALYVADGHHRSAAAS
                     RVGEILKKKNPKHTGEEMYNYFLTVIFPGNQLRIMDYNRVVKDLNGLSPEEFLKKISD
                     KFGVSPTGDPRPAQKGDYGMLLGNKWYRLTAKPGSFPSDDPVQSLDVSILQNNLLDPV
                     LGIKDPRTDVRIDFVGGIRGLKELERRCKLDSAVAFALFPTGVEELMAIADAGQIMPP
                     KSTWFEPKLRDGLVVKMLNE"
     gene            11008..11700
                     /locus_tag="PKK36_03030"
     CDS             11008..11700
                     /locus_tag="PKK36_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93564.1"
                     /translation="MNRNAYRRLKWVWLIGHCAMPFLAVLYFRHVPVREMIPPILFHF
                     WPAVLTFCMVFWLIFEALLLMYRFPLDVRDALVLVGVFVLQYAFMFWGSGSAGYAAFT
                     SIVCTLIAMGICAALLTVKTLSSMNRDMRLAAGLIIVLVFTPAAVFMPILLYGLRGMT
                     SALKILNIAFMLNAVVVAVRELVSLSVFGKPDAQQAAYDEEWQKWAAPTIILLILSAA
                     GAAVMGGIMNAQ"
     gene            11767..12498
                     /locus_tag="PKK36_03035"
     CDS             11767..12498
                     /locus_tag="PKK36_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HNR93565.1"
                     /translation="MMNVAGPAFEELDYQQTPMGELILQRRTILALNNAEAYEVRLNN
                     EYLMSSLFHEAEEVMSDLALAELSGDGWEVVVGGLGLGYTAASALKYPRVGRLIVVEA
                     LEPVIDWHRRGLVPNGKQLSEDSRCVFLHADFFALARGEGFAPGSPHHRFDAVLLDID
                     HAPDFLLNPAHADFYTEAGLQRLKNLLKPGGVFALWSNDPVDPGFLGRLQAVFDRAEG
                     HLVPFENPLIQSTSSNSVYIACRNK"
     gene            12730..13914
                     /locus_tag="PKK36_03040"
     CDS             12730..13914
                     /locus_tag="PKK36_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR93566.1"
                     /translation="MSSSSSDKKRIAKLVREMPRSGIRDFFDIISTRHDIISLSIGEP
                     EFETPESIRKVAATALQTGAQATRYTSNMGLLELRRALAHYVKKMFNAEYDPEKEILI
                     TTGVSEAMDLAMRAIVDPGDEVIYHEPAFVSYRPEILFAGGVPVPIVTTEEDGFRLKP
                     ELVEAAITKKTKALLLNFPNNPTGAMLSRQDFEKLAKICIKHDLMVITDEIYAELSYG
                     DEYVSIASIPGMKERTIFLHGFSKAWAMTGFRMGYSCAPAALTEAMMKIHQYVMMSAP
                     TISQIAAIEALSHAEIDTEQMRKGYQERRDFMYDSIREMGIPCTKPGGAFYMFPRVGV
                     LGLSSMDFAMNFLNEESVAVVPGTAFGACGEGYVRCAYVASMQNLKLAMMRMKRFVDK
                     LR"
     gene            complement(14287..15075)
                     /locus_tag="PKK36_03045"
     CDS             complement(14287..15075)
                     /locus_tag="PKK36_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR93567.1"
                     /translation="MKILVLNGSCRSGGTTEELARAFMDGASESGAEVEMVMLRDQTI
                     QYCTNCLKCYSYDGDGTAPCSLHDDMDDIIRRMVEADGILFASPVHNGFVTGLMTVFW
                     ERLSWRVARPDGPFLNMMSIISRIDGKVRAFGSIASAGGMPERLRRFCDDGTPWLKGN
                     APLILHGQWVGDMYAGADLARQPEGRKDWQRLYFLRRISSRQKQRARDLGMRMADAIR
                     SGRLKPVTMEKMVPPLLGKVLQRLFTASPPYHLAEEPAPGSPAG"
     gene            complement(15104..15385)
                     /locus_tag="PKK36_03050"
     CDS             complement(15104..15385)
                     /locus_tag="PKK36_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93568.1"
                     /translation="MLVEKVKGDYRFTSPTTDGAFMVMLTLTSKKEELLNTVSVALSA
                     SFPQRKLKPGTIRTAIGEERWQNFLRMKKLKLRSMFIDPHDHNFMPQRF"
     gene            15562..16263
                     /locus_tag="PKK36_03055"
     CDS             15562..16263
                     /locus_tag="PKK36_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNR93569.1"
                     /translation="MSQKKTYTIACPKCKAAQEEELYESVNVAEEPLLKESLLQNRIN
                     AVTCAECGFSFRVDKPLLYHDPLHSAMIYYLPLGGADYDKGERHFREFLGEMHTLMPD
                     GLDLPDVHLVFSWAELVERVFLLEADLDPRLIEYVKYIIYTKNSERLKPEQKAILFNA
                     EDSTAEQFCFVIQDVATRKLESILHYSREAYESLCETFDQDEQTPMLLEMFPGPYVSA
                     RALLLRETAEEDEDA"
     gene            complement(16320..16994)
                     /locus_tag="PKK36_03060"
     CDS             complement(16320..16994)
                     /locus_tag="PKK36_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNR93570.1"
                     /translation="MKRIFIPATFILILILAAPAASQARVPRGKRPRVLIIGDSISMG
                     YTPYVQRIFRGTAVVRHHRGNAGPTARGLEDIEKWLGRKKWAAIHFNWGLWDMYGWKH
                     EGHDQTPETYERNLDTLVARLKQTGAVLIWATTTPACPEAEEESGLIIGPKGEAEYLD
                     AAARVMKKHGVQINDLHALIKPVQPKYARGENDVHYTKDGYKLMAGQVADSIRKALNL
                     SGPSTE"
     gene            complement(17005..18132)
                     /locus_tag="PKK36_03065"
     CDS             complement(17005..18132)
                     /locus_tag="PKK36_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93571.1"
                     /translation="MENFREVCALLLGCVIVASSSKAMEPEAALLLRLSRSDSSPAFA
                     QRDLPRSGRAGEDELEGAARLEIGMRTEPRDDLHLKFSVMSAARWGSEDDSIIEAGES
                     FAALENIAMMPLSVSAGRQAISWGRGLLFSDQYGEHLFDMAGGRWDTLPHRFDFIYAD
                     PALTVFDSPVNHLWLARWLYQAEGRSSVEMFGGAMSLENDGHAAIAGLRGVWAAGPAW
                     ETWAELAGEAGDRSSNRNLEALIADCGIIRRFSKGEIRTAWTWASGSDDEVHSFVPLF
                     NGEHWGRLYDPPLSGIHIFTASASRDMTEALKISGEYFSYRQGDSGEPLGSEVDLSAR
                     CALSDAVSLELNGCVFFTDDGLRSMNDNTRTEIRLQLQAVF"
     gene            complement(18142..18819)
                     /locus_tag="PKK36_03070"
     CDS             complement(18142..18819)
                     /locus_tag="PKK36_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93572.1"
                     /translation="MSGEEKKENLDVQEPVTAAPADKEEVQQVMDLVREYAVPAAIAL
                     GLVLVVILGFTAWRGYKKGLAERAATGLLTARTVQDLENVVSQFPETPSAPAALLTLA
                     AEQLHSGQYLNAQNAYGRFEMQFPNHPLRPVARIGTAYCMEGDGRLAEADAAFRAFAA
                     EYPDHYLAGVAVIGQARCQEQMNNLEGARKIYDDFIAANPDSPWLQQAKTALLYLEKN
                     MRVAQKP"
     gene            18995..19525
                     /locus_tag="PKK36_03075"
     CDS             18995..19525
                     /locus_tag="PKK36_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93573.1"
                     /translation="MLKWKMTVLGLCLGVLAAAGSDVRAAARQPQRLTMLVVPANPAQ
                     LELAFDVADRFPAVLVSYQTRKKSDEPLLHAWNGSEWVYLSFESYQRGDFMTRVPSRA
                     ILVGDETLLPAALVTASAWCPSVMNIPDLNPAVILNSLSRVFTFTENDWRWFARRYNL
                     ELKDLNAKRRRTSWYD"
     gene            19522..19842
                     /locus_tag="PKK36_03080"
     CDS             19522..19842
                     /locus_tag="PKK36_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93574.1"
                     /translation="MKIYSVILLLALVGCVSTVVPTEPLAESVLIVTRSGEQVFLQWA
                     SEPGIHYTVFYTDSSSAGRRWLPLPAAVKLRGTGEDIKISDSVPTTVQRRYRLQVDMP
                     VRAK"
     gene            complement(19846..20364)
                     /locus_tag="PKK36_03085"
     CDS             complement(19846..20364)
                     /locus_tag="PKK36_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93575.1"
                     /translation="MKIKALAEAAGRSVPYVMNIQKKFGMRQMLNYPEGHAALLTKLV
                     RLSLCSVPQKDIELLLARERKLLEMLKVDSVSEDSDWFERLCTMKTGPTRLLLSGYDL
                     KCRIDGNAVQPGLDFAERTRELFTEKELASDALTALRNYAAIFRKIQGQLIQDIPAMA
                     ASVNWARRIAAR"
     gene            complement(20465..22708)
                     /locus_tag="PKK36_03090"
     CDS             complement(20465..22708)
                     /locus_tag="PKK36_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-K-Cl cotransporter"
                     /protein_id="HNR93576.1"
                     /translation="MTIESQSKEEPQGYSFGAFKGVFTPSILTILGVIMYLRFGWVLG
                     QVGLPLTLLIVTMASSITFLTALSLSASATNMKVGGGGAYYIISRSLGVEVGSAIGVP
                     LFLAQALGVAFYITGFSEALAGVVNVQPLIEQLPFEVSEIRLISILTLAILAIIALIS
                     ADLALKTQMLILAAIFASLVSFFLGHPPAADAPAQSAVDITQRVSFWAVFAVFFPAVT
                     GIEAGLALSGDLKDPAKALPRGTIAAVLAGYLVYMAIPPVLLHFVPDSGRLLAEPMIM
                     QSVARWGALILVGVWAATLSSALGSLLGAPRTMQALSRDGVFPRFLGRGFGAKNDPRI
                     ATAVTFLVALTAILLGDLNMLAPVLSMFFLTSYGLLNLSAGLEGLIDSPTWRPQFRVK
                     PALSLAGAAACFSVMFMINAGATFAAIVVVGFIYALQKRRNLTARWGDMRYGLLNMLA
                     EYAIRRLSLKQPDERTWRPNILVLSGSPQSRWYLIELAHALVHNACGMTVATVVSGEK
                     WIADRIRSLQASIAEYLIKRDVHALVKILPEDDILDGATALIKAYGYGPMRPNTILMG
                     DCEEWGNVERFAELVINIYRMNRNLVLVREVGEEPVTPETRYIDIWWRGQQTNIGMML
                     TLAYMMKRSEVWSGADIRLKRILDADADVNEVTAAIEEHTRVHRLDVTVDFVKRDREN
                     VFDIIRESSRDAALVFMGLRAPEPEETAADYAAYYQRFHRATAEMPVAYTLAGESVDF
                     EQIIGLS"
     gene            22912..23967
                     /locus_tag="PKK36_03095"
     CDS             22912..23967
                     /locus_tag="PKK36_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR93577.1"
                     /translation="MMQIPKVIGGIWGKVNLPVRTAGLYALLGGLWILFSDRAIGLFT
                     NDPRRLTFLQTYKGWGFVLVTSLLLYFFLLREWSMLQKEIQARRLAEDKLRRLNAELE
                     ARVALRTDELARAKERAESADRIKSAFLATMSHELRTPLNSIIGFSDIMGEGLTGPLN
                     DEQKKQLGMVQASARHLLALINDVLDISKIEAGQLTLSFSEFELRDALQRMVELVTPL
                     ARKKGLELRVSVADDVGTVSSDQRRLEQVILNLLANAVKFTDRGHVAVSCRAEGGHYL
                     LTVSDTGVGIRPEAVPGLFQPFHQIDSTLARRHEGTGLGLSISKKLVEKMGGTIGVES
                     EWGRGSVFTVRLPVRQV"
     gene            24065..24247
                     /locus_tag="PKK36_03100"
     CDS             24065..24247
                     /locus_tag="PKK36_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93578.1"
                     /translation="MKKLLMWVSMAAVLLSTGVLSGCRQEEKTAAPAAAEEKMDAEKT
                     SAEHPARQKPKDHPAH"
     gene            24286..>24741
                     /locus_tag="PKK36_03105"
     CDS             24286..>24741
                     /locus_tag="PKK36_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR93579.1"
                     /translation="MIMRSDAWIVLFGCVLLSGCFGDKTGPAESAQTGTFPIVTGDIQ
                     AGIEKHIAEQIEQGDGYFVVPFEGRELKLRLVRVHVEYLASLAPQRHFACVDMASADG
                     QFYDIDFFLAGDPGSMTVTETTVHKLNGQPYYLWKQNEDRNWVRAPVDDA"
BASE COUNT         4919 a         7189 c         7449 g         5184 t
ORIGIN      
        1 aaaaaagttc caatgattgg aagtttttgc cgggattttt ccagtcattg gaacttttgg
       61 gagaggggtt tcccggatgg agaaaatcag gccgcggcgg aggctttgcg gattttttca
      121 agggccgttt cgaaatcggc ggcagagacc agatagaagt ggcactcgcg acccgtgacc
      181 gccttgatgt tttcctgcag atccttgtta aagggattga ggatcgccac aagaagatcc
      241 tttgccatct ggtcgaaggc aagcgcgccc tgccgcacca tgacatccat cggaatcagg
      301 ttgaaggctg cgggctgcag ttcgaaagcc gtcagggtga tcagcggcat gcccgccgat
      361 ttggccaggt aggcgaggat gcgctcgcgg tgcttgtact gccggtcctc cagcacatgc
      421 agcacggtca ccggactgtt cagggccttg gtggagtttt ccgtgaggtc gtgtacaacc
      481 tccgtgtatt cgtcctgcgt gatttcgccg gcctgggcca gttcccacgc cagcgacatt
      541 tcattgctta tatcaagcct ctttgcgggg gccctgccgc gggtttccgc ggccgccggg
      601 gtgtttgccg ccggaggttt tgcgctgccc tgaagctgct gtatggtttc gagaatttcc
      661 cgtgccgccg gatctttccc tcccagcgag gtcagtttcg gctgcaggcc gatggcgccg
      721 gctgtgtcac cctcctgaag catgaccttc gcaagccgca ccagatagtt cagggcctga
      781 gcctgatcgc cgatcttctc atatgcctcc tgaagcgttt caagcgccag gcggtcttcc
      841 ggcatggctt caatgatctg ttcaaatgcg gagatggcat tccagacttc ggtttcggca
      901 tcgagcttct tcttctgatc agccatagtg ttccaccctg tgtttgcccg gcggcagacg
      961 cttccgccgc gagtgattac acgtaaaatc atgtaaaagt gcaacatgtt tttgtgtgca
     1021 ttgcgaaaac gatatgctaa cttaatgcct gcttgaagat gcggatccgg tctgcggggc
     1081 ggccgcgatg gttttatgac agctcgggag taacggcatg gctgcaggaa aaatgtcgaa
     1141 caaggtcgtt gatattctga tcaagcgcca caagctggat ccggaagggc tcgacaatct
     1201 gatgcaggaa gcctctgagg cgggcgcgcg tcttgaggac tggctggtga agaagaaggc
     1261 cgtttcgcag gcggacatga cgctcgcggt ggcggaatac ctccggatgc cccccatcga
     1321 actgcagaat ttcgtgccgg accccaagct gcttgatctg attccgtcaa aaatcatggg
     1381 tcagcatctg ctggtgccga tgtgccgctg tcacaacatg cttacgattg cactgggcga
     1441 tccgtttgat gtgaccgcgc aggacgaggt gcatgccctc accgggcttg atgtttttcc
     1501 cgtggtcgcc gcagaagccc agatcaaggc gctgattcag aagcacacgc aggacgccgc
     1561 cacggggctg gaagacatta ttcgcgacgt gaccgagggc gacattgagc tgtccaaaac
     1621 tgaagaggag caggtcagcc ttgatgagat gctggaggag gccgaggacg cgcccgtgat
     1681 ccgcgtcgtg aactcgatca tggtggaggc catccggcga ggagcgagcg atattcacat
     1741 tgagccgatg gagcgctcta tccggctgcg ctaccgcatt gacggcgagc tgtacgaaag
     1801 cccgagtccg cccaaaccgc tgcaggcggc gatcatttcg cgcctgaaga tcatgtccaa
     1861 cctcgacatc gccgagcggc gcattcctca ggacggccgt ttccgcattc gcgcgctggg
     1921 caaggaggtg gatatccgcc tgagcattct gccctgcgtg cacggtgaaa agatcgtgat
     1981 gcgtactctg gacaaaactt cactggcacc cagtctggcg gcgctgaacc tcgagaagac
     2041 cgcctatgag aacctgatgt tcggcatcag ccagccgcac gggctgattc tggtcaccgg
     2101 acccacgggc agcggaaaaa ccaccacgct ctattccgcc ctgcaggagc tgaacaccac
     2161 ggatgtgaac atcatcacgg tggaagaccc ggtcgaatat cagctggcgg gcatcaatca
     2221 ggttcagacc caggcttcgg tagggctcac cttcgccgcg gccctgcgct cgattctgcg
     2281 ccaggatccg gacattgtgc tcgtggggga aacccgtgac ggggagaccg ccgccattgc
     2341 cattcaagcg gccctgacgg gccaccttgt gctgaccacg ctgcacacga acgacgcggc
     2401 gggcgcggtc tcgcgtcttc tctacatggg cattgaaccc ttcatgctgg cgtcctcgct
     2461 gatcatggcg caggcccagc gcctatatcg caaactctgc ccggtatgca agcagcccat
     2521 agatattccc acggaaactc ttcgcctgaa ccatctggac ccggccaagt ttgagggggt
     2581 tcagtttttc taccataagg gctgccccaa gtgcggaaac atcggataca agggccgcgg
     2641 cgccgtgatg gaaatcctga tggtcaatga ccacatccgc aagctgattc tcgacaacgc
     2701 ggactcggcc gccgtgcgga cccaggcggt ttccgacggc atgagaacgc tgctggatgt
     2761 cgggctggac cgggtccgtg atggactgac aagcattgaa gaggccctga gcgtggcggg
     2821 cggcgaataa cggagacaaa ccatggcgga aagaacaacg ggaaccggcg cccatatcgg
     2881 cggcaaggtt aaaaaaatca gaattcccgg cgcgaaaaaa atcgcccgga ttgaactgcc
     2941 cgctttcacg cgccagcttg cagcgatgct ttcgtccggc atgcccgtgg tgcagacgct
     3001 ctcggcgctt gaggagcaga tcgaaaacac aaacttcaag acggtcatcg gcggcgtgcg
     3061 cgtgcagatc gagggcggct cctccttcac cgacgcgctc cgcaaatatc ccgatatatt
     3121 cgatgatctg tacgtcagcc tgatgcaggc gggtgaaatg ggcggtttga tggctgaaac
     3181 caccgcgcgc attgcggcgt ttcttgaggc gcgcgagcgc ctccgccgca aggtgaaatc
     3241 ggccatgacc tatccggtga tcgtgctcac cattgtgttc ggcatcacgg ccggcctgat
     3301 catttttgtg gtgcccaagt tcgccgccat tttctcggac ttcggcgcca agcttccggg
     3361 cccgacacag tttctggtgg acctgagcga aggggtcaga aaatacgccc tgtttgtggt
     3421 gctgggcatc gtggcctttg tgatcgcctt cggaaaattc aaaaggaccc cgaagggcgc
     3481 ctatatgctg gatgccctga atctcaaact gcctgtgttc ggcgaactga caacaaaggt
     3541 ggctctggcc cgttttgcct cgacctttgc gcagctcatc cggagcggtg tgcccatcct
     3601 ccagtcgctg gacattgtcg ccttcgccac aggcaacaag gtgctgggcg aaaccatcat
     3661 gaaggggcgc gacagcgtgg agcggggcga gcccctttcc aatgcgcttt ccgccgaccc
     3721 caagttcccg cgcatgcttg tccatatgct gtccgccggc gaaaagaccg gccgcatgga
     3781 ggacatgctt cagaagatcg ccgaattcta cgacgaagaa gttgaagcca tgctggacgg
     3841 catgaccgcc ctgattgaac cgctgatgat tgtggtcatg ggcgtggtgg tcggcggcat
     3901 tgtgctgtgc ctcttcctcc cgatcttcaa gatgtccgaa atcgtgtcca tgtgattcaa
     3961 cgggagaagc gccgtgccga acagagaagc aaacagaaaa accacggtgg gggcggttga
     4021 tgcggaggtt cttgccttta cggccggccg cgatgccgtg ctcgaccttg ctctggccga
     4081 agcggactgc atcggatccg ccgctcatgt gaccatgctc tcccgcatgc gggtgaagcc
     4141 gcgtctcttc tcggatcagg accgccgggc ggtgattggc gaactggtgc agatcatgaa
     4201 atccgcgcgc agggggcgct ttcagatcac ggtagaggac caggatgtcc atctggccgt
     4261 tgagcgtttg ctgacggcga agctgggcgt gctgggcaag cggattcata ccggccgaag
     4321 ccggaacgac caggtggcgc tggatctccg cctctatgcc aaaacccgcc tgcaggccgc
     4381 cgtggcccgg gcggcggagc tggcgcgcga actccttttg tttgcccgcc gccatcagaa
     4441 ggtgcccatg gccggccgca cgcacatgca gcccgcgatg ccgtcgtctg tcggactgtg
     4501 ggcttccgcc catgcggaaa gcctgctgga agacatcatg ctgatgatta acgcctatga
     4561 gctcaacgac cagtgcccgc tgggcgcggc ggcgggctac ggggtgccgc tcccgctgga
     4621 ccgccagctg gtttccgatc tgctcggctt cagccgaccc tgccagaacg tcctttatgc
     4681 ggtcaccgcc cgcggcaagc tggagtccat cattctctcg gcgctcagcc aggcgatgct
     4741 gaccctctcg cgcctgtcgg aggacatcgt catctattcc atgccggaat tcgggtattt
     4801 ccggctgccg ccggcctttt gcaccggcag cagcatcatg ccgcagaagt tcaatcccga
     4861 tgtgctggag ctgatccgcg cgcgcgccgt gcgtgtggcc tcagacgcgc aggcggtcgc
     4921 ggccgttgtg cgcggcctcg gttccggata tcaccgcgac gtgcaggaga ccaaggagcc
     4981 cctgatgaac ggcatggaca caacagagtc cgccctgcgc atcatgaccc gcttcattcc
     5041 ggcactgaag gttgaccgtg ccgcgctgaa aaagggcttt gttcccggtg tgttcgcggc
     5101 cgaccgcgcc ctggcgctgg tcgcgggggg catgcccttc agggaagcct atcagcacgt
     5161 caaggaccac ctggatgagc tttccgccgc tgatccggac agggcgctgg cggcaaagat
     5221 cagtctgggc gctccagcca atatagactt cgccgccctg cttgaacgcg caaagcaggc
     5281 ctccgcctgg gccgccgacg agcagaaatc cttcaacaaa acgatcagca gactgctggg
     5341 acagaagtat cccctgtcgc cgcagagatg aatccatggc cggcacggaa tatccgatag
     5401 aacttgatgc gctggccgtc agctatcctg ttcacggtgg acaggtggat gtgctcaggg
     5461 gaatcagtct caaggtgggg cggggacaga tcgtcggctt tctcggcccc aacggcgcgg
     5521 ggaaaaccac aaccatccac gtgctgctgg gttttgtcga gccggccgcg ggttctgccc
     5581 ggctgtttgg cgaagatgtg cgcaggacca ttgcacgcca gcgaatcggc tacctgcccg
     5641 agcatccgca gacctacggg tttctcaccg gacgggagct gctgcggaca gccggccggc
     5701 ttttcggcat gcgggggcca tccctggaaa accgcgtgaa agaactgctc gggacggttg
     5761 atctggaatc ggccgcggat cgccgcatca gcacctattc gcgcggcatg atgcagcgca
     5821 tcggcgtggc gcaggcgctg atcaatgatc cggacctggt cattctggat gaacccaccg
     5881 gggggctgga ccctctcggc cgcatgcaga ttcgtgccat catcggcgac ttgagaaccc
     5941 gggggaaaac agttttcttc tcgtcgcacg agctgtccga agtggagcgt gtgtgcgaca
     6001 gcgttgtgat gctggtgggc gggcgcatag cggcggccgg cccggtgtcc gaactggtgc
     6061 agccgtccga gagtctggag agctacttcc tgcgcgtggt ggaaaggggt ctggcttcat
     6121 gatccgcatt ttgaccatag cgggggcggt ctggcgggaa atgctccgcc gcagggattt
     6181 ttacgtgctg ctgatcctgc tgggcgcgct tctattttcg ctgatgagca tcaatgtttt
     6241 cggccttggt tcaaccatgc gctacatggt ggatacgggc cttctgctgg cgtggatatt
     6301 ttccgctgta ctggcggtga cgctttccgc ccgccagctt ccggcggagg agcagcgggg
     6361 cacgatcttc gccctgcttg ccaagccggt cacccgctgg cagcttctcg cgggcaaggc
     6421 gcttggcgca tggtccgcgg tatccgcggc gaccgccgcg ttttatgcgc tgctgctggc
     6481 cgtgctggcg ctgaagggct ggtcggttga tctgacggtg acgctgcagg cgctggtgct
     6541 gcatgtcacc gctctggcca tggctgtttc gcttgcgctg gctgtttcca cccgcaccac
     6601 gttcggcgcg gccgcatcac tgacatggct tgcgcttgcc gcgggctttg tttttgttcc
     6661 ccagattccc gcgctgctgg tatactccga ccgcgtttcc ggcgcggcca tgctggtgct
     6721 gtattatgcg cttccgcatt ttgagctttt cgatctgcgg cagtgcgtgg tgcacgactg
     6781 gccggcgctg cccgcgaaca tcctgtttcc cgtgctctgc tacggccttc ttgtcaccgc
     6841 gattttcatg ctgctggcgt ggctgggcta tcgcggccgg catttcaagc gcggagatgt
     6901 cctgtgaaag atgctcgttc agccgccctg aaaaccgcat ggccctggct gctgctgctc
     6961 gcgctgtgga cggcggcctt tgcggcggtc tgcagtcttc cgcaggaggc ctgggcgcac
     7021 gccggcgagg gcgcctccgt gaccgaccgg cttcttggat ccacacgcat gattatcggc
     7081 gaggcgtttt atgagcagag cgataattac tttcatcgcg gcgttccgca tcagaagaaa
     7141 aaggcgtttc acgatatatt tcagacctgg gcgcatcatg tgcggcccgt atcgcatgag
     7201 catgccacgg gaaagtccgt ctatgaggtg atgccgtggc tggatttcac cgtgcggatg
     7261 aatccgcatc acgtggaggc ttatctggtg gcggcctact ggcttgcagg tgatggggag
     7321 cgtcccgatc tggccatgca gattgttgcg gacggtctgc gcgccaatcc gggcgactat
     7381 cagcttcatg ccgagcgcgg ccggctttat ctgcagcagg gcgaaaacga ccgggctgaa
     7441 aaagcgctgg aaaccgccgt gcggctgtgg ccctccggtt atgatcccga tgacccgatg
     7501 atccgctatg atctggcacg aagcctgagc tatctttcgg tcctgcgcca ggcggcaggc
     7561 cgcctggagg ctgcgtccga aagcgcggcc cgtgcggcgg cgctcttccc ccaaaacaca
     7621 tcgcttcgcg cgcgggcggg cgagcttgcg cgcgggaccg cgacaatgga cgccgcccgc
     7681 cggaccattg aggctctttt cccggtgcag tcaaaagcgg gccgccacgt atgcagccgg
     7741 ggtgaaaatc acgatcatga gaatgaacac gaacttgagc attgaggccg gctgagatat
     7801 gtatttggat tattatcagc tcagggaaat gcctttcaac atcacgcccg atccgcgttt
     7861 cctctacttt accggccgcc atcgcgaggc attcgatcat ctgctctacg gcattgaaca
     7921 ccgcaaggga ttcattcagc ttaccggaga agtcggttcc ggaaaaacca ctctctgccg
     7981 cgcggtgctc tcgcggctcc cgacgtccat ccggaccgcc ctggttctga atccgtgtct
     8041 ttccgaaacc cagctcctgc gggccatcat aaacgacttt ggcctggatc cggccggcaa
     8101 cgaccggctg gcctatattg agcagctcaa cgaattcctg ctgggccagc tggaggaggg
     8161 atacaacgtt gctctcatca ttgacgaggc gcaggatctg gaaccggccg tcatggagca
     8221 gatccgcctg ctgaacaatc tggagacgga tcagcacaag ctcatgcaga tcgtgctcgt
     8281 gggccagccg gaactggagg agaagcttgc gcgcaatgaa ctccgccagc tgcgccagcg
     8341 catcaccgtg aaggcccatc tgtcggcgct gtctgaagag gaaaccgcgg actatatttc
     8401 gcaccgcctg gaagtggcgg gcgccgccgg ccggattcag tttgacggcg gggccatccg
     8461 ccagctgcac aaaatagcgg gcggagttcc ccgtattgtg aacaccattg ccgaccgcgc
     8521 gctgatggcc ggctacattg tcaacacatt caccatcggg gcaaaggaag tgaagagagc
     8581 catccgcgac ctggagttga agccatgagt ctgatacagg aagcactgcg acgcaaagag
     8641 gcagatgaca aaggggttcc tcctccgccg cccgtgccgt ccccggcgcc gaaaaaggac
     8701 agccccctga aggcggtgat cgctgtgctg gcggtgctgc ttgtgctgat cggagtcgcc
     8761 gtatatttgt tcacctatct ggtcaattcc ttcacgccgc gccggattgc gccgcccgct
     8821 ccggtgcaga tgcctgaagc ggtgaagccc gcgcccgtgc aggttgtgga aaagacgccc
     8881 gaaccggaac cgcctgaggt tcttgaatcc gaaccggtcg cggccgccgc tcctgtccgg
     8941 gaaattccag aacccgcgcc ggaaccggtt gtgaccgaag ctccccgccc cgaacctgtg
     9001 ccggaacccg tttcagttgc cgcggccgag cctgtcaggc tggaggtaaa aaccgtggcg
     9061 caaccggcga ccgtcgaatg gcccaacctg aacctgacgg gcgtattgcg aaaaggcggc
     9121 tccggcgggc ccgcctccgc catgatcagc ggagagctcg tgagtgtcag cgagaccatc
     9181 cggggcgtga gacttgtcgc ggtggaggcc agcggcgtat ggctggagct caacggcgag
     9241 cagaaattcc tcgccatcgg ccggtcgctg aaatagccgg cgtccggcta ttcattcagc
     9301 attttgacaa cgaggccgtc gcgcagcttg ggttcaaacc atgtgctctt gggcggcatg
     9361 atctgacccg catcggcgat ggccatcagt tcctccacgc cggtcgggaa cagcgcgaag
     9421 gccacggccg aatccagttt gcaacgccgc tccagttcct tcagcccgcg aatgccgccc
     9481 acgaagtcta tgcggacatc cgtgcgggga tctttgatcc cgagcaccgg gtccagcaga
     9541 ttgttctgca gaatggatac atcgaggctc tgcacagggt catcggaagg gaagcttccg
     9601 ggtttggccg tcaggcggta ccatttgttg ccgagcagca tgccatagtc gcctttttgg
     9661 gccggccggg gatcccccgt cggtgaaacc ccgaacttgt cggagatctt tttcaggaac
     9721 tcctccggcg agaggccgtt caggtccttc accacgcggt tatagtccat gatccggagc
     9781 tggttgcccg gaaagatcac cgtgaggaaa tagttgtaca tctcctcgcc ggtgtgtttg
     9841 ggatttttct ttttcaggat ttcgccgacc cggctcgcgg ccgcgctgcg atggtggccg
     9901 tcggccacat aaagtgcggg aaccttcttg aactccgtct gcaaagcggc gatatcggcc
     9961 tttgcatcca cgacccagag cgtgtggccg atgccgtcgt ccgccgtgaa gtcatagacg
    10021 ggcttctgtg cgcagatccg gccgatgatg gcatcaattt ccggccgcgc gcggtaggtc
    10081 aggaagacgc atccggtgtg cgcgccgagc atctcaacgt gatgtgtgcg gtcatcttcc
    10141 ttgtctttgc gggtcagctc atgctttttg atcagatcgt tctggtattc tgcaacgctc
    10201 ccgccgcaca gcaggccggt ttgaacatgc tcgcccatgc gctgggcata gacatacatg
    10261 cagggctccc tgtccttgat cagcaggccg tcggcgacca tcttcttcag gttgtcggca
    10321 cccctctgat agaccacatc ggagtgcacg tcgatggacg gatcaaggtc cacttcggac
    10381 ttggtgacat gcaggaaaga gagcgggttt ccccggacgc gttcgcgggc ttcgtcggag
    10441 ttgaggacgt catagggcgg actggcgatc tgggaagcgg catcggcacg cggccggtac
    10501 ccgcagaagg ggcgaatatc tgacatgagc aattcctttc ttgaatatta atccctgccg
    10561 gcggggcgaa agcggtcccg gccggaaagc cgaaaatcaa aaagggcaga cgcccgccgc
    10621 ctcagatcac cggcgcggac ttcatcatct tcttctcccg cttggcctga cgcttctcgg
    10681 cgaggctctt ctggggggcc tttttcaaat tcttctttgc atcatgcgat tttgccatgg
    10741 cggtatctcc caattatgaa tttcacagta cgcagtgtgc tgttctatgg acaggaagct
    10801 accgaaacac ggggtttccg gcaagcgcaa accggcggct tttgcgggcg cggagaaagt
    10861 tccaatcatt ggaacttttc gtcaaaaaac ttccaatggt tggaactatt ttagcggaaa
    10921 cttccaatga ctggaacttc tggggttgat atatcggata ccgatatatt gcggtccggc
    10981 ccggtaaagg gtagaagttg tggccagatg aaccggaatg cgtacaggag attgaaatgg
    11041 gtctggctga tcggccactg cgccatgccg ttcctggccg tgctgtattt tcgccatgtg
    11101 ccggtgcggg agatgattcc cccgatcctt tttcatttct ggcccgcggt attgacgttc
    11161 tgcatggtgt tctggctgat ttttgaagcc ctccttctga tgtaccgctt cccgctcgat
    11221 gtccgcgatg cgctggtcct cgtgggcgtt tttgttcttc agtacgcatt catgttctgg
    11281 ggcagcggat cggccggcta tgcggccttc acttcgattg tctgcactct gatcgccatg
    11341 ggaatctgcg ccgcgctgct gaccgtgaaa accctgtctt ccatgaaccg cgatatgcgg
    11401 ctggccgcgg ggctgatcat tgtgctggtt tttacgcctg ccgctgtttt catgcccatt
    11461 ctcttgtacg gtctgcgggg aatgacatcc gcgctcaaaa tcctgaacat cgccttcatg
    11521 ctgaacgccg tcgtcgtggc tgttcgggaa ctggtcagcc tttccgtctt cgggaagccg
    11581 gatgcgcagc aggccgccta tgatgaagaa tggcagaagt gggccgcccc gaccattatt
    11641 ctgctgattc tctccgccgc cggagcggcg gtgatgggcg gcatcatgaa tgcgcagtag
    11701 gcgtcctgcc gcggatgaac cattttgctg tggcctgtat cctccgccgg tggtatggtg
    11761 gcggccatga tgaatgtggc tgggccggca tttgaagaac tcgactacca gcaaaccccc
    11821 atgggggagc tgatccttca gcgaagaacc atccttgccc tcaacaatgc tgaagcctat
    11881 gaagtccggc tgaacaatga gtatctcatg tccagcctgt ttcatgaggc ggaagaggtg
    11941 atgtccgatc tggcccttgc ggaactgtcg ggcgatggct gggaggttgt ggtcggcggg
    12001 ctgggcctgg gctatactgc ggcgtccgca ctgaaatatc cgcgggtggg ccggctgatt
    12061 gttgtcgaag cgcttgaacc cgtcattgac tggcaccggc gcggtctggt ccccaacggc
    12121 aaacagctca gcgaagactc gcgctgtgtt tttcttcatg ccgatttctt cgcgctggca
    12181 agaggcgaag gctttgcgcc cggaagtccg catcaccgct tcgatgccgt tctgctggac
    12241 attgatcatg cgcccgactt cctgctgaat cccgctcacg ccgattttta tacggaggcc
    12301 ggactgcaaa gactgaaaaa ccttctgaaa ccgggcgggg tgttcgcgct gtggtcgaac
    12361 gatccggtgg atcccggctt cctcggccgc ctgcaggcgg tctttgaccg ggccgaaggt
    12421 catctggttc cctttgaaaa tccccttatc cagtccacat caagcaacag tgtgtacatc
    12481 gcctgcagga ataaataaag gccgggtcaa tccctccacg acaaatcgga gaaaccctga
    12541 aaaccggctg aaggaagccg ataaacggaa catggccgag agacggtatg gagaggggaa
    12601 tttgccctaa aactccacgg acggcatgga catgcgcagt tgatttcttt tcgatccatg
    12661 cgctctcagt gctggactgg gccgggcacc cagtgtaatg tcgcggtttg attcaggagt
    12721 ttggaagtta tgagcagttc atcgtcagat aaaaagcgta ttgcaaagct tgttcgggaa
    12781 atgccccgtt cgggcattcg tgattttttt gacatcatca gcacccggca tgacatcatc
    12841 agcctgagca tcggcgagcc ggagttcgag acgcccgaaa gcatccgcaa ggtggccgcg
    12901 acggcgctgc agaccggagc gcaggccacg cgctatacgt ccaacatggg tctgcttgag
    12961 ctgcgccgcg cgcttgcgca ttatgtgaag aagatgttca acgcggagta tgaccccgag
    13021 aaggaaatcc tgattaccac cggcgtcagc gaggccatgg acctcgccat gcgggccatt
    13081 gtggatccgg gggacgaggt gatttatcac gagccggcgt ttgtttcgta ccggccggaa
    13141 attctgtttg ccggcggcgt gcctgtgccc attgttacaa cggaagaaga tggtttccgc
    13201 ctgaaacccg aactggtgga ggccgccatc acaaaaaaga ccaaggcgct gctgctgaat
    13261 tttccgaaca atccgaccgg agccatgctt tcgcgtcagg atttcgaaaa actggccaaa
    13321 atctgcatca agcacgacct gatggtcatc accgatgaaa tttacgccga gctttcctat
    13381 ggtgacgaat atgtcagcat tgcatccatt cccggcatga aggaacgcac cattttcctg
    13441 cacggatttt ccaaggcgtg ggccatgacc ggcttccgga tgggttattc ctgtgcgcct
    13501 gcggccctga ctgaggctat gatgaaaatt catcagtacg tgatgatgtc cgcgccaaca
    13561 atcagccaga ttgccgccat cgaggcgctt tcgcatgcgg aaattgatac cgagcaaatg
    13621 cgcaagggat atcaggagcg ccgcgacttc atgtatgatt ccatccggga aatgggcatc
    13681 ccctgcacca aaccgggcgg agctttttac atgtttcccc gcgttggagt tctgggattg
    13741 tcctccatgg actttgcgat gaacttcctc aacgaggaaa gtgtggccgt tgttcccgga
    13801 acagcctttg gcgcctgcgg agaaggatat gtccggtgcg cctatgtggc cagcatgcag
    13861 aacctgaaac ttgccatgat gcgcatgaag cggtttgttg ataagctccg ctgaaccccg
    13921 tttttggccc ccccataata cgctaataat ccgcaacttg cgcgggataa aaagacattg
    13981 cgcggcggct ctttatacac agagaccaca gatgattgtg aatatcatca cgagtggaac
    14041 ccgccatttt cccagtagta atactttcat aataaaagag taatgagaaa agatctgccg
    14101 gctcacgggc cggttcgcac aagttgtcca cagcccgcct gcccaacgga gaaaagaatt
    14161 gcttgacaga aaacagagga tttcgtgtcg gacaatatcg gcatggaacg gccgctgaaa
    14221 acagcggcat ccgcctgttt tccaccacgg cggccggccg attttggccg cctcacagtg
    14281 cggatatcag ccggcggggc ttccgggggc gggctcttcg gcaaggtgat agggcgggct
    14341 cgcggtaaag aggcgctgga gaactttccc cagaagcggg ggaaccatct tctccatggt
    14401 aacaggcttc agccggcccg agcgaatggc gtccgccatc cgcatgccca aatcccgggc
    14461 cctctgcttc tgccgggagc tgatccggcg caggaaatac agcctctgcc agtcttttct
    14521 gccttccggc tgtcgggcca gatccgctcc ggcatacata tcgccgaccc actgcccgtg
    14581 cagaatcagg ggggcgttgc ctttgagcca gggggttccg tcatcacaga atctgcgcag
    14641 ccgttccggc atccctccgg cactggcgat tgatccaaaa gcgcgcactt tcccgtctat
    14701 gcggcttatg atgctcatca tattcagaaa aggtccgtcg ggccgggcca cccgccagga
    14761 aagacgctcc caaaacaccg tcatgagccc ggtgacaaat ccgttatgga caggtgaggc
    14821 gaaaagaatg ccgtccgcct caaccatccg gcggatgata tcgtccatgt cgtcatgcag
    14881 gctgcagggg gcagttccgt ctccgtcgta actgtagcac ttcaggcagt tggtgcagta
    14941 ctggatggtt tggtcgcgaa gcatgaccat ctcaacctct gcgccgcttt ccgaagcccc
    15001 gtccataaac gcccgggcga gttcttccgt cgttcccccg gagcgacagc ttccattcaa
    15061 aacaagaatc ttcatcggtc agccaccttt gtccgaccgg ggcctaaaag cgctgcggca
    15121 tgaagttgtg gtcgtgcgga tcaataaaca tggaccggag tttcagtttt ttcattcgca
    15181 ggaaattctg ccagcgctct tcgccgatgg ccgtgcggat ggttcccggt ttcagcttgc
    15241 gctgcggaaa cgaagcggac agcgccacgg ataccgtgtt gagcagttcc tcttttttgg
    15301 aggtcagcgt gagcatgacc atgaaagccc cgtccgtggt tggacttgta aaccgatagt
    15361 cgcctttaac tttttccacc agcatgtgac gcctcccttg ttctatcact gttccggaga
    15421 tgcacccgtg cattccagac gacagtatag cggccggaga gggcgggggg aagggaaaag
    15481 gctgttagaa attacgaccc gcatgcaact tcccggtgtt tgctgttgat gccgcccggc
    15541 gatgtgcata gtatgcggac catgtctcag aaaaaaacat atacaattgc ctgtccgaag
    15601 tgcaaggcgg cgcaggaaga ggagctttac gaatccgtga atgtggcgga agaacccctg
    15661 ctcaaggaaa gcctgcttca gaatcggatc aacgcggtga cctgcgcgga atgcggtttc
    15721 agttttcggg tggacaagcc gctgctgtat cacgaccccc tgcattcggc gatgatctat
    15781 tatcttccgc tgggcggcgc ggactatgac aagggggagc gccatttccg cgagtttctc
    15841 ggggaaatgc acaccctgat gcccgacggg ctggacctgc cggatgttca tctggtcttc
    15901 tcctgggcgg agctggtcga acgggtgttt ctgcttgaag cggacctgga tccgaggctg
    15961 atcgaatacg tgaagtacat catctacacg aaaaattcgg aacggctcaa acccgagcag
    16021 aaagccatcc tcttcaatgc agaggactcc acggccgaac agttctgttt cgtgattcag
    16081 gatgtggcca cccgcaaact ggagagcatt ctgcattaca gccgcgaggc ctatgaatca
    16141 ctctgcgaaa catttgatca ggacgaacag acgccgatgc ttctggagat gttccccggg
    16201 ccctatgtga gcgcgcgcgc cctgctcctg cgcgagaccg ctgaagagga cgaagacgcc
    16261 tgaccggggc tgctccggga tctgcgcccg gagattcaat atcgaacgta tcattaggtc
    16321 tattctgtgg agggcccgga aaggttcaac gcttttctga tcgaatcggc gacctggccg
    16381 gccatcagtt tatagccgtc ctttgtataa tggacatcat tttccccgcg cgcatatttc
    16441 ggctgcacgg gcttgatcag ggcgtgcagg tcgttgatct gcacgccgtg cttcttcatg
    16501 acccgcgcgg cggcatcgag atattccgct tcccctttcg ggccaatgat cagccccgac
    16561 tcctcctcgg cttccgggca ggcaggcgtt gtcgtggccc agatcagaac ggcaccggtt
    16621 tgtttaaggc gcgcgaccag tgtgtcgaga tttctttcat aggtttccgg ggtctggtca
    16681 tgtccttcgt gtttccatcc gtacatgtcc cacagacccc agttgaaatg gatcgccgcc
    16741 cattttttgc gacccagcca tttttcaata tcttcaagac cgcgcgcggt cggtcccgcg
    16801 tttccgcgat gatgtctcac aaccgccgtg cccctgaata ttctctgcac atagggggtg
    16861 tatcccatgg agattgagtc gccaataatg agaaccctcg ggcgcttccc gcgcggcacc
    16921 cgggcctgtg acgcggcagg tgcagccagt atgagaatca ggatgaatgt ggcgggaatg
    16981 aatattcttt tcatggaata actctcaaaa cacggcttga agctgaagcc ggatttcggt
    17041 ccgggtgttg tcattcattg agcgcaggcc gtcatccgtg aagaacacgc acccgttcag
    17101 ttccagtgac acggcgtctg acagcgcgca gcgggccgac agatcaactt cgcttccgag
    17161 cggctcgccg ctgtcgccct gccggtaact gaagtactcc ccggagatct tcagcgcctc
    17221 cgtcatgtcg cgcgatgcgg atgccgtgaa gatatggatt ccgctcaggg gcggatcata
    17281 gagccgtccc cagtgctccc cgttgaacag cggaacaaag gaatgaactt cgtcatcact
    17341 gcccgaggcc caggtccatg ccgtgcgaat ttcccctttt gaaaaacggc ggataattcc
    17401 gcagtcggca atgagggcct ccagatttct gttcgagctt ctgtcaccgg cttcaccggc
    17461 cagttcggcc caagtttccc acgcgggacc tgcggcccac accccgcgta aacccgcaat
    17521 ggcggcatgc ccatcattct caaggctcat ggcgcctccg aacatttcga cggaacttcg
    17581 gccttcggcc tgataaagcc agcgggccag ccagagatga ttcacgggcg agtcaaacac
    17641 cgtcagggcg gggtcggcat aaataaaatc aaaacgatgg ggaagagtat cccatctgcc
    17701 gcccgccata tcgaacagat gttccccgta ctggtcgctg aacagaagcc cgcggcccca
    17761 cgaaattgcc tggcggccgg ctgaaactga aaggggcatc atggcgatgt tctcaagggc
    17821 tgcaaaggat tctccggcct cgatgatgga gtcatcctct gatccccatc gagcggcgga
    17881 catcacagaa aatttcagat ggagatcatc ccgcggttcg gtcctcatcc cgatttccag
    17941 ccgcgcagcg ccttccagtt cgtcttcacc ggcccttccg ctgcggggga gatcgcgctg
    18001 cgcgaaggcg ggggacgagt cggagcggga cagtcgcagg agcagggcgg cctcgggctc
    18061 catggccttt gaggaagagg cgacaatgac acaccccaga agaagcgcgc agacctccct
    18121 gaagttttcc attcgcacga actacggttt ctgcgccacg cgcatatttt tttcaaggta
    18181 gagaagggcg gttttggcct gctgcagcca ggggctgtcg ggattggccg cgatgaaatc
    18241 gtcatatatt ttccgggcgc cctccagatt gttcatctgc tcctgacagc gcgcctggcc
    18301 gatgaccgcc acgccggcga gatagtgatc cggatattct gcggcaaaag ccctgaaggc
    18361 tgcgtcggct tcagccaggc gtccatcgcc ctccatgcag taggccgtgc cgatccgggc
    18421 cacggggcgg agcggatgat tggggaactg catttcgaaa cggccatagg cgttttgcgc
    18481 gttcaggtac tgtccgctgt gcagctgctc ggctgcgagc gtgagcagcg cggccggagc
    18541 ggagggcgtt tcagggaact gtgaaacgac attttccaga tcctgcaccg ttcgcgcggt
    18601 cagcaggccg gttgcggccc gctcggcaag gcctttcttg tagccccgcc aggcggtgaa
    18661 tccgagaatt accacaagca caagtcccag ggcgatggcc gcaggcacgg cgtactcgcg
    18721 taccagatcc atcacctgct gcacttcttc cttgtcggcc ggcgccgcgg tgacgggttc
    18781 ctgaacgtcc agattctctt tcttctcttc tccgctcatg gcagttctcc tgaatacctt
    18841 tgcataaagt ccggtattta tggccttact gcatcaaaaa ggcaagaccg catgtgttaa
    18901 atcgggtttc cgcgaaactc aatatcgcgg cttgagttct gcgggaagac atgctaatgg
    18961 tcagatgcca attttgaaga aaggggcgga tgccatgttg aagtggaaaa tgacggtatt
    19021 gggtttgtgc ctgggggtgc tcgcggctgc gggttccgat gtcagggccg cggcccgcca
    19081 gcctcagcgg ctgacgatgc ttgtggtgcc ggccaatccg gcgcagctgg agctggcatt
    19141 tgatgtggcc gaccggtttc ctgccgtgct ggtgtcctat cagacgcgca agaaatccga
    19201 cgaaccactg ctgcacgcgt ggaacggctc tgaatgggtt tacctctcct tcgaaagtta
    19261 tcagcgcggc gatttcatga cccgtgtgcc ttcgcgcgcc attctggtgg gcgatgaaac
    19321 cctccttccg gcggctctgg tgacggcttc ggcctggtgt ccctccgtta tgaacatacc
    19381 ggacctgaac ccggcggtca ttctcaactc gctgtcacgg gtgttcacct tcacggagaa
    19441 tgactggcgg tggtttgcgc gccgctacaa tctggaactt aaagacctga acgccaaacg
    19501 ccgccgcacc agctggtacg attgaaaata tattccgtca ttctgctgct ggccctcgtc
    19561 ggctgtgtga gcacggttgt tcccacggaa ccgctggcag agtccgttct tattgtcaca
    19621 cgcagcgggg agcaggtttt cctgcagtgg gcttccgagc ccggcattca ttacaccgta
    19681 ttctatacgg actcctcctc ggccggccgg cgctggcttc cgcttcccgc tgccgtgaaa
    19741 ctgcgcggaa cgggcgagga catcaaaatt tccgacagcg tgccgacgac cgtgcagcgc
    19801 cgttaccgcc tgcaggttga catgccggtg cgcgccaaat agggatcatc gagcggcaat
    19861 tcttctggcc cagttcaccg atgcggccat cgcgggaata tcctgaatca gctggccctg
    19921 aatttttctg aagattgccg catagttgcg aagagcggtc agcgcatcag aggccagttc
    19981 cttctccgtg aacagttccc gggtgcgttc ggcgaaatcc aggcccggct gcacggcgtt
    20041 tccgtcaatg cggcacttca ggtcgtagcc gctcagcagc aggcgcgtcg gcccggtttt
    20101 catggtgcac aggcgctcaa accagtcgct gtcttcggag acggaatcca ccttcagcat
    20161 ctccagaagc tttcgctcgc gggcgagcag gagttcgatg tccttctggg gaacggagca
    20221 aagagaaagc cggaccagct tcgtcagcag cgcagcatgc ccctcgggat aattcagcat
    20281 ctgccgcatg ccgaattttt tctgtatgtt catcacatac ggaactgatc tgcctgcggc
    20341 ttcggccagc gctttgattt tcatccgtgc acgactcctg tttccccgca tcatatgaaa
    20401 tgaagccggg caaaacaatt tcaaaacagc attccgtgaa aagcgggaaa aaagcgggcc
    20461 gggatcagct gagaccgatg atttgttcga aatccacgga ctccccggcg agcgtgtagg
    20521 ccacgggcat ttccgccgtg gcccggtgga accgctggta ataggcggcg tagtctgcgg
    20581 ccgtttcctc gggctccggc gcgcgcagac ccatgaaaac cagggctgca tcacgggatg
    20641 actcacggat tatgtcgaac acattttccc ggtcccgttt cacgaaatca acggtgacat
    20701 ccagccgatg gacgcgggta tgctcctcaa tggcggcggt cacctcgttc acgtccgcat
    20761 ccgcatccag aatgcgcttc agccgaatgt cggctccaga ccagacttcg ctccgcttca
    20821 tcatataggc cagcgtgagc atcattccga tgttggtctg ctgaccccgc caccagatgt
    20881 caatataacg tgtttccggg gtgaccggtt cctcgccgac ctcccgtacc agcacgagat
    20941 tgcggttcat gcgataaata ttgatcacca gttctgcgaa acgctcgaca ttgccccatt
    21001 cctcgcagtc gcccatcaga atggtgttgg gacgcatggg tccgtagccg taggccttga
    21061 tcagcgccgt tgccccatca agtatgtcat cctccggcag gatcttgacc agggcgtgca
    21121 catcccgctt gatcagatac tccgcgatgc ttgcctgcag ggagcgaatg cggtcggcga
    21181 tccatttctc tccggagacc acggtggcca cggtcatgcc gcaggcgttg tgcaccagcg
    21241 catgggccag ttcaatcagg taccagcggg actgggggga cccgctcagg acaagaatgt
    21301 tgggtcgcca ggttcgttcg tccggctgct tgagagagag cctgcgaatg gcatattccg
    21361 caagcatgtt gagcaggcca tagcgcatgt cgccccatcg cgccgtcagg ttgcggcgtt
    21421 tctgcagtgc atagatgaag cccaccacca caatggcggc aaaggtggcg ccggcattga
    21481 tcatgaacat gacagaaaag caggctgccg cgcccgccag cgaaagcgcg ggtttcactc
    21541 ggaactgggg acgccatgtc ggactgtcaa tcagtccttc cagcccggct gaaagattca
    21601 gcaggccgta ggaggtgaga aagaacatgg ataaaacagg ggccagcatg ttgagatcgc
    21661 ccagcaggat ggcggtcagc gccacgagga aagtaacggc tgtggcgatg cggggatcgt
    21721 ttttggctcc aaatccccgg cccagaaaac gggggaaaac tccatcccgg ctcagcgcct
    21781 gcatggtgcg tggcgcgcca aggagcgaac ccaaagcgct tgagagtgtg gcggcccaaa
    21841 ccccgacaag aatcagcgct ccccagcgcg ccacggactg catgatcatg ggttcggcca
    21901 gaagcctgcc gctgtcggga acaaaatgaa gcagtaccgg gggaatggcc atatacacca
    21961 ggtatcccgc cagcacagcc gcgatggttc cgcgcgggag ggctttggcc ggatccttca
    22021 aatcgcccga cagcgcgagc ccggcctcaa tgccggtgac ggccggaaag aagacggcga
    22081 atacggccca aaacgatact ctttgggtga tgtccaccgc gctctgggcg ggggcatcgg
    22141 cggcgggcgg gtggccgaga aaaaaggata ccagcgaggc aaaaatagcc gcgagaatca
    22201 gcatttgtgt tttcaacgcc agatcggcgg aaatcagggc gattatcgcg aggatcgcaa
    22261 gagtcagaat cgaaatgagc cggatttcgc tgacttcaaa cggaagctgt tcgatcaaag
    22321 gctggacatt caccaccccg gcgagggctt cggaaaaccc ggtgatgtaa aaggcaacac
    22381 ccagggcctg cgccagaaac agcggaaccc cgatggccga acccacttca acgcccagag
    22441 agcgggagat gatgtaataa gctccgccgc cccccacctt catattggtg gcggaagcgg
    22501 aaagcgagag ggccgtcagg aaggtgatgg atgaggccat ggtcacaatc agaagcgtca
    22561 ggggcagccc cacctgcccc agcacccagc cgaagcgcag gtacatgatc acgccaagaa
    22621 tggtcaggat gctcggcgta aaaacgcctt taaacgctcc gaacgaatag ccctgcggct
    22681 cttctttact ctgactttcg attgtcacgg ccgtaaattc aacgctctga atatgtccga
    22741 tatgtactgg aacaggccgg caatgacaac tggaatcgaa aaagcatcca gtgccgtgca
    22801 ccatgcgcgg ccccggcgga gcattgactc tatttgtgca acatggcaaa atgcacaaac
    22861 agtctgaaag gacatgggcg gtgaaggccg ggcattgccc cgagggagcg catgatgcag
    22921 attccgaagg tcatcggggg gatttggggg aaggtgaatc ttcccgttcg caccgcgggg
    22981 ctgtatgcgc tcctcggggg tctctggatt cttttttctg accgcgcaat tgggctgttc
    23041 acaaacgatc cccgccggct caccttcctg cagacctaca agggctgggg tttcgtgctg
    23101 gttacgtcac tgctgctgta ttttttcctt cttcgggaat ggtccatgct tcagaaggaa
    23161 atacaggccc gccggctggc cgaggacaaa ctgcgcaggc tgaatgcaga actggaggcg
    23221 cgcgtcgcgc tccgcacgga tgagcttgcc cgcgccaagg agcgcgccga atccgctgac
    23281 cggatcaagt cggcctttct ggctactatg tcccacgagc tgcgcacccc tctgaattcc
    23341 atcatcggct tcagcgatat catgggcgag ggtttgaccg gccccctgaa cgatgaacag
    23401 aagaagcagc tcggcatggt gcaggccagc gcccggcacc tgctcgcgct tatcaatgac
    23461 gtgctggata tttcaaaaat tgaagccggg cagcttacgc tctcgttttc tgaattcgaa
    23521 ctgcgcgacg ccttgcagag aatggttgag ctggtgacac ccctggcgcg gaaaaagggc
    23581 cttgaactca gggtgtctgt tgccgacgat gtgggcacgg tgagttcgga tcagcgacgg
    23641 ctggagcagg tcattctcaa cctgctggcc aacgccgtga aattcactga ccgcggccat
    23701 gtggcggtgt cctgccgcgc ggagggcggc cattatctgc tgacggtttc cgacacgggt
    23761 gtcggaatcc ggccggaagc tgtcccgggc ctgtttcagc cgtttcatca gattgacagc
    23821 accctggcgc gccgacacga gggaacgggc ctgggtctgt ccatcagcaa aaagctggtc
    23881 gaaaaaatgg gcggaaccat cggggttgag agtgaatggg gccggggaag tgttttcacc
    23941 gttcgtctgc cggtgcggca ggtataaaat ccgttcccgc ccgcttgcta ttgcggcggt
    24001 tatcgcgtag tttgttattc tcgcatgggt tctgtccctg cggaggaaag agaagggaaa
    24061 gcatatgaaa aagctgctga tgtgggtgtc catggccgca gttctgctgt cgaccggcgt
    24121 tttgagcggt tgccggcagg aagaaaagac tgccgcgcct gccgccgcgg aagagaagat
    24181 ggacgccgag aagacgtccg ccgagcatcc ggctcggcag aaacccaaag atcacccggc
    24241 gcactgaccg tgtcaggcgg gagaaaacaa agccggggga gcggcatgat catgcgttcc
    24301 gatgcatgga tcgtgttgtt cggctgtgtt ctgctgtccg gatgctttgg agacaaaacc
    24361 ggtcccgcag agtcggcaca aaccgggaca ttccccatcg tcaccggcga tatccaggcc
    24421 ggcatcgaga agcatatcgc cgagcagatt gaacagggtg atggctactt cgtggtccca
    24481 tttgagggcc gggaactgaa gctcaggctg gtgcgggttc atgtcgaata tctggcctca
    24541 ctcgcccctc agcgccactt cgcctgcgtg gacatggcga gcgcggatgg ccagttttat
    24601 gacattgatt tctttctcgc gggcgatccc ggctccatga cggtgacgga gaccaccgtt
    24661 cacaagctga acggccagcc ctactatctc tggaagcaga atgaggacag gaactgggtg
    24721 cgcgcgccgg tggatgacgc c
//
LOCUS       DAOQWR010000021        24331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2410_length_24331_cov_5.392404, whole genome shotgun sequence.
ACCESSION   DAOQWR010000021 DAOQWR010000000
VERSION     DAOQWR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24331
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_24331_cov_5.392404"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3268)
                     /locus_tag="PKK36_03110"
     CDS             complement(<1..3268)
                     /locus_tag="PKK36_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR93580.1"
                     /translation="MIKSDSRHLIFTLLAVFACALSIPLVAGAEEDGIGDWGVNDPVY
                     GLKTREDESGFTYTPSPADWRDINIYQLFTDRFADSGEDQLAGYKPAWKCEGKSFPYN
                     RNYHHGGDWKGLKNNLGYLTGMGVKAVWMSGVQMNDQGKDTNYTPYHQYHPENFFKCD
                     PAMGTFQDLKDLIDACHAAGIYVILDVAPNHMCDKNGLWGNNQLEDKQYWASGNSTFG
                     WWDQNNKHPAPFDNLNYFHNNGTINCWDCSPENLLGQFKGTDDLKTEDAAVQSILSKA
                     FKNLIDATDCDGFRVDAIKHVEYNWIKQWADDMRKHAAYRGKNDFIIFGELFSYDNGA
                     LASFCKDTGYSFNSALFFPMSQTIKGVFVDNGWAGQLTQQLNNKSQYGEGADRLVAFI
                     DNHDLNRIALMNGGDAGNDVWKLRPALSFLYLATPVPCLYYGTEHVFDQGGHYNGSSP
                     SGDNPDDGDWQRECMFDKGFQPGPAQGNKLAATTAPLYLHIAALNTARETYKSLTRGS
                     FQERWTDGAYAFSRVYDDEEALVAINLGDGNKDLNPEVGKDSTEFTNVLNPAEKVTSS
                     GGRLSFSLSGKETKIFVAGTAAPALWARGTHSYPVAGEITSDTPIYINSEAGPAGSVT
                     NVLLGYSLNGGTTWTTMVMAVNTNWSSQGGAWYNATLGPQPADTVVEYYIEAQGEADS
                     RAWDNNNGQNYTFTVAQGSSSGLWIRGTKNYPEDGDATFATDLYVDTEAGPAGSVTGV
                     QVVHSTNGTSWATNAMTLNADWGSDGGSWYNANLGTFAEGTVVRYFIQATDGEETVES
                     NNGGLYFQVTIRGAGLAITNPPGDLAVENSVSNYTLQGTASAVTNGNLRWTNTLTGAS
                     GQIAAASPWSVENILLGVGANQIIVSGETSGGAPSAMQDSADNYTGGWADGANQGSGF
                     GPWSFNHTQDGTTFWAGCFIGNPTNAGIEGFGENAFGFYANPPASGANAEVLRSFSSA
                     MTTGSVFSFKLGLNYDSNAEGSNRGFSLLAGDTELVNINMGNSQTVTVNGASMFTNYG
                     YRAMTMNFEYASSGSIHVWANGRDGIESYDQTLSVPAGAPTRFKFYFNATDAGTDERQ
                     M"
     gene            3447..3947
                     /gene="tpx"
                     /locus_tag="PKK36_03115"
     CDS             3447..3947
                     /gene="tpx"
                     /locus_tag="PKK36_03115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNR93581.1"
                     /translation="MATVKLKGNPAETSGNLPRPGSAAPAFELAKSDLSNARLADFSG
                     ETLVLNIFPSIDTPVCAASVRRFNEEAGNRNNVAVLCISMDLPFAHARFCEVEGLKNV
                     TPLSAFRSPAFGKDYGLTITTGPLAGLLARAVIILDPKGRIIYSELVPEITQEPDYKA
                     ALNVLP"
     gene            complement(4012..6504)
                     /locus_tag="PKK36_03120"
     CDS             complement(4012..6504)
                     /locus_tag="PKK36_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR93582.1"
                     /translation="MKECLRSAVCVLFRVCRQRGPAFAFFFFFVGNVFIRCASAGPLA
                     PDTITVVSDDNYPPFIFRDDGGGLRGILPDEWKLWEQKTGVRVNLMAMDWNLAQEYMR
                     LGRADVLDTIFSNSVRSSIYDFTRPYADIEVPVFSHHSLGGFADIESLRGFPIGVKAG
                     DACIDVLKAGGVKDFREYPSYEAIIQAASDRDILVFSVDKPPALYYLYKMNLEDDFRL
                     SFTLYTGAFHRAVAKGRLDVLQLVEDGFAQITEDERRAVMNRWMGRSLSHPAYIRPLI
                     YAILATAGFCAVLLVFNLMLRHKVEEKMADLKRVEQKGRESEETFRAVIESSPMGIYL
                     YRLEDDSRLVLVGANPAADRIIGMDHSVYFGKTIEEVFPPLAKTEIPDRYRRAAQFGE
                     SWRSEQVDYDYGGIRGAFEVYAFQIAEGEAAVMFNEISERKQAEAERERLREQLAQAQ
                     KMESVGRLAGGVAHDFNNMLGLILGHVELAMDQLDPADPVCDELREIHNAAERSADLT
                     RQLLAFARKQPIQPRRLDINATVEGLLKMLKRLIGENIELVWRPGVQIPPVMMDGSQI
                     DHLLANLCVNARDAIADTGRITITTGLARLRGAACAAHPECAPGDYVELSVSDNGCGM
                     DAEALTRLFEPFFTTKEVGRGTGLGLATVYGIVRQNHGFIDVDSRPGHGTRIRICLPR
                     CDGDIDVPGPKERSEEAAPTGGATVLLVADEATILKLGARMLRKLGYQVMEADRPDKA
                     MQAVREYQGRIDLLITDLVMPRMNGRELADQIRQLRPDIRVLFTSGYAAGTLDNLAPG
                     HEAEISFLAKPFNMASLSRSVHSALSRKRSGR"
     gene            complement(6585..7148)
                     /gene="frr"
                     /locus_tag="PKK36_03125"
     CDS             complement(6585..7148)
                     /gene="frr"
                     /locus_tag="PKK36_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNR93583.1"
                     /translation="METLDDILLDSDDKMSKALEYLQQELAGLRTGKASPSLVDHIAV
                     DYYGAATRLREIAGISTPEPRLIVINAYDPSALPAIEKAILAANIGVTPISDGRLIRI
                     PIPELTEERRRELTKVAKRQAEEQRVAVRNVRRDANEAIKNLQKSSKITEDERDSGLE
                     EIQKYTDDYIKKIDALLAAKEKDIMQV"
     gene            complement(7166..7876)
                     /gene="pyrH"
                     /locus_tag="PKK36_03130"
     CDS             complement(7166..7876)
                     /gene="pyrH"
                     /locus_tag="PKK36_03130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015769891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNR93584.1"
                     /translation="MKYKRVLLKLSGEAFLDGDKSESLSPEMLQKAARQLKKVHDAGI
                     QLAVVNGGGNIFRGLSGLEGGIDRARGDNMGMLATMINALALQSALENLGVETCVMSG
                     VEMPRIAEAAIAAKAVGHLEKGRIVIIGGGTGNPYFTTDSAAALRACEIKAGALLKGT
                     KVDGIYTADPVKDPSAVRYDDISYKEALNKRLKVMDSSAFSLCMENDIPIIVFNWFKD
                     DEIVRVLKGEPVGTVVHQ"
     gene            8032..8790
                     /locus_tag="PKK36_03135"
     CDS             8032..8790
                     /locus_tag="PKK36_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNR93585.1"
                     /translation="MDRKDLSLITEGWDYNPREVRARWVKTASGEAEVQLRLDLGVLQ
                     MRAEGRPDGQTPRGFPSLLDYYEELERTVPEDHPSLQFDEDMCAELQQEAVQYYYRYL
                     AYSALGHLDGVIADTDHDLSIFDLVAEHAIDDDVAWQFIQFFPYVRMMNSKALAEKGL
                     REKNYDEALAAVEEGLCDIRNFMQDYTDEDEDFEDCPEIRTLQSLQHKIIRMQPRSEE
                     DRIHEELAASIASENYEHAARLRDRLKKLGKTPF"
     gene            8825..9274
                     /locus_tag="PKK36_03140"
     CDS             8825..9274
                     /locus_tag="PKK36_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR93586.1"
                     /translation="MKKQIPWELYSRIIQNVPIACVDVAIVANGAVLLVRRKDDPAKG
                     QWWLPGGRVLKGEMMKAAAARKARDEVGIECHVGPIVHTAETIFPDGPGGISIHSINS
                     CFFMYPVKADFKPSLDSHHTDAKWVRRIPKGVHPYVEQCLMGAGLSK"
     gene            complement(9314..9823)
                     /locus_tag="PKK36_03145"
     CDS             complement(9314..9823)
                     /locus_tag="PKK36_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR93587.1"
                     /translation="MDALQAIFSRRSIRKFTRQPVAGDQIEILMKAASVAPSAGDQRP
                     WHFVVITDTAVLRKLSAAMPKCEMLDTATLGILVCGDEALEKIPGYWVQDCSACAENI
                     LLAAHALGLGAVWIAIHPIPDRFNPCREILNVPENIFPFALIAAGHPNENLQGEDRLD
                     PSRIHNNLW"
     gene            10000..10329
                     /locus_tag="PKK36_03150"
     CDS             10000..10329
                     /locus_tag="PKK36_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HNR93588.1"
                     /translation="MKLYQVLSILAVVSVALAAGCSKGTTEQAAPAAGVAAASDEGFV
                     AIDSSLLEKVKYDGAAQTLTVVFDSGETYVYAGVPQAIYDGLMAAPSKGSFFEASIKD
                     AFQFEKK"
     gene            10444..11070
                     /gene="plsY"
                     /locus_tag="PKK36_03155"
     CDS             10444..11070
                     /gene="plsY"
                     /locus_tag="PKK36_03155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNR93589.1"
                     /translation="MQSDWLSVLAAAAGSYLLGSVPFGLLLARARGVDIRSVGSGNIG
                     ATNVFRAVGKGPGLLTFFLDFLKGLLPVLAAGHWLAPDTAAPILCGCAAIAGHIWTVF
                     AQFRGGKGVATGAGVVVGIAWEAVIIGLICWIIVFALTRYVSLASIITAGSVGISGWI
                     LYPGRRLVPATLTLLAALVIVRHRSNIVRLLKGTENRFDFKKKKEGAE"
     gene            11067..12068
                     /locus_tag="PKK36_03160"
     CDS             11067..12068
                     /locus_tag="PKK36_03160"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNR93590.1"
                     /translation="MKRVAVIGDGGWGTALATVLQANGHHVTVWGPFADYIGEIMAAR
                     ENRKFLPGVKLPPEIQWTADRAEAAKNADAWVLAVPSKFYEEVVASFRPFADKNTHAI
                     SVTKGLDRKTHRRMSEMAADLLRGGPIVALTGPSHAEEVARGIPTAVVAACEDHARAE
                     FVQELFSNPRFRVYTTDDTVGAELGGCLKNVIAIAVGVSDGIGFGDNTRAALITRGLA
                     EMKRLGCALGAHAETFSGLSGMGDLIVTCTSRLSRNRAVGEKLGRGQSIESILAGMEQ
                     VAEGVANSASALALAEAHGVEVPITQEVNDVIHRGKNPKLAVQALLTRDPRPERDSP"
     gene            12065..12640
                     /locus_tag="PKK36_03165"
     CDS             12065..12640
                     /locus_tag="PKK36_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR93591.1"
                     /translation="MKIHLGIVLAAGASSRMGSPKALLRPPDGIPLALHQIRLLESGG
                     CSKTAIVLGAEADRIAPELPGVRIILNPAWESGRPSSVLAALKGAADCEGMIILPVDT
                     VGIRPETIKRVLEFSDTAEWPAVRPVCRGIRGKLVWISSALSDEIIQKNYSERLDDLL
                     ADRAFELEVDDPAILRNINTPKDWEEVSGQL"
     gene            complement(12643..13494)
                     /locus_tag="PKK36_03170"
     CDS             complement(12643..13494)
                     /locus_tag="PKK36_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNR93592.1"
                     /translation="MKKLEKSDCRFPSTLKEALELLANESTRGRLLAGGSDLMVQWAA
                     GMEPPPRVISIKGLKELQGIKEDDGAVVIGAGVTHAHIRSSKPVQKHLPALAAAAATV
                     GGPQIQALGTIGGNVANASPAGDLAPALLITGGEAVVASRTGERTIPLTKFFLAYRRI
                     DLKPDEMIVRFVLPKRAAGEIEVFRKLGTRAAQAISKVMCAARFSVKAGSFETCAVAL
                     GSVGPTTVRLPKLEEWIIGKSASEETLAEVEKRASDEVSPIDDIRSTAEYRKWVSGRL
                     VRFLLES"
     gene            complement(13497..13976)
                     /locus_tag="PKK36_03175"
     CDS             complement(13497..13976)
                     /locus_tag="PKK36_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR93593.1"
                     /translation="MSNVKRTWNVNGEEKTLEFPPLRRLLDVIREDMRFTGCKEGCGE
                     GECGACTVLLDGVPVTSCLVAAGQVPDGTQILTVEGLEKTEQGRVLQEAYVSAGASQC
                     GFCIPGMLMASFALLSKNSRPTEEDILDAHGGNICRCTGYVKIIEAVRLAAQNWPRK"
     gene            complement(13980..16289)
                     /locus_tag="PKK36_03180"
     CDS             complement(13980..16289)
                     /locus_tag="PKK36_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNR93594.1"
                     /translation="MGPKRKIKSGHVGADSTRVDAPDKVRGRAVYTDDIDVPGCWYGK
                     VVRSPVAHGRLRSLKRDEEFDWSRVSVVLPDDIPGKNIADLFIHDMPFIAHDEINYLG
                     EPLALVAAPDRELAEEAARHFHPDIEELPGIFHMEEIVDLYKKQDSSLHSLGEQTTVK
                     GDIEKGFSEADMIVTGEYWAGYQEQLYIEPQAMIAEMMDDGGVFIRGSLQCPYFINPE
                     LCLTLNLPPEKIRVKQEMTGGAFGGKEEYPTLIAGYCALLAMKSRHPVKIVFDRNEDI
                     LFTTKRHPSWGRLKTGLKKDGTITAIHAELLLNGGAYTTISRVVLARGILHAAMGYRC
                     DNVFVDGRIFKTHTFPCGAFRGFGAPQATWAFESHIEELAKACGMLPQEFRRKNCLVQ
                     GDVTPTGMVLKDSVGSPAVLDQVLDRSDFAKKLKQSSRGNPKHKKWYGIGLAFFAHGS
                     GFTGDGEARFKAKGAVELDWIEPNHPGVNIRISSTEMGQGAFTIMDQIAADAISIPLD
                     DVRCPLPDTSLCPNSGPTVASRTTMVVGHTVHGAGLKLKAALEEFASKEVFEGRPVVL
                     ENGRFVCSGEALPFRQIAASYLDKHGRLRTEHQFTLPPDLKWDQSTFKGDAYPAFSWG
                     CNAAEVEVDPLTFEIRLKKVTACFDVGRMINPLNCKAQAEGGLVQTIGYALMEKMGIR
                     NGKYDADRMQTYIVPTMPDIPEFDITFVEYPYEFSPPGAKGIGEMPMDGLAPAIANAI
                     ENATGLRCNRIPIMPEDLFTLHRAKHQNG"
     gene            complement(16426..20019)
                     /locus_tag="PKK36_03185"
     CDS             complement(16426..20019)
                     /locus_tag="PKK36_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93595.1"
                     /translation="MTKYTLMSVLTAALAFAGNLMGATYTDSAANYPGGWTNESNGGT
                     GFGPWSVVADAQGGFAGHGIWDSAGAGLNMGLAFGYVGKVGYINIDRDFSQALNTGDT
                     FALDFGVNWDSSGGNKGFSLYALDEEVINVNHGSFPGEIYMNGNVALAEFGTNTMRWT
                     FTQEAADQIAVQATGRDGSETFSATVTVANGYGYLGSMRFYSSGLPDTLEDQRQSYFD
                     NLELNQEGTPPPDPLILTFTAGDANPGALGDYEYTLGRIGEVGDDIDLSSDNEDALTV
                     PASVSFETGVDSLTFTAAVVSVTNGAATITATNSATGASATYVVNIQVPTLQVNGPAA
                     AWTNSTKWYTVTRLGMVDDTVNLSSSNTNVLSVPATVDFPSGQNSVSFPAVIGMAGAA
                     QITATNASAASAPFEVTVTETPNVIAHDEAGNYTPATFTNGSNEGLGLGPWNFWNKDA
                     ELGDSTAGGGGDLNSTNGYSFRFMGDGSNGWCNASRNLGGALRVGNVLSFTFTYNWDG
                     GGRGVDISSQSGQFANLIDVTPGNVFKVNQQIISTAYSPGAVVNVEITQLAGGIEVYL
                     TRSVTGGVNLAYTTNIVHGEPATSVAMYCGGYTADPIGDNANYAIFMNNLQIAGDERV
                     SLVFSDGTWNPGALGDYEFVIERSGPVGSNIVLSSSNTNAVTVPGSVQFEEGSNTVSF
                     LATVVSLTAGEATLIASNEETGAWAEYEVKPALPVLYIAGPWELTVPGIPAEYTLTRE
                     GGVGDDIILSSSDEGVLTVTNSAAFGGGSTSLVFEATPVWFGTATLTASNAASGAFAT
                     YDVTVQAPELTITGRDDIWTGGTTETYTITRKGAVPDTIFLGSTNTAVMTVPASVDFQ
                     SGSNSLTFEATVLAAGQTRLTANDGGDASTYLDVTVTERPEFDAYDDSSLYEGGVWSN
                     SPAHVTGFSDWTETLSPETNDSYRGKFIGASAIGGINEGGTAFGLYANYTGAQPDPLP
                     EIKVSREFPAPMVSGQTFSVDVAYNYSGGTKGFKLKGDFEGTPYERFELYNSGGDTWS
                     YKLDAGTPVVIWSNYLAGGFMGRVQAICTAPNTFTFSFLRGAEETPVLVEDVVLPGTI
                     DQIEFYNYNGGSGDEENFYFNRMYVTKAPAGPPEIGEIVPGAGGSSFTFDVPAGYTLG
                     AVYGADTVLEDGSWDWELLVENTDYTVSGGQVTILGDAAAHRIIRIGVNPE"
     gene            complement(20088..20894)
                     /locus_tag="PKK36_03190"
     CDS             complement(20088..20894)
                     /locus_tag="PKK36_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93596.1"
                     /translation="MTQASLYCVCLDFDGTIMEYEGELGWFHPAVIECLNALEAAGIQ
                     WFTNSGRGIRNQLEVLDLSVKRGLVHRPEKMICGESFIYDRSGSGYADREPWNTDTRA
                     YLRRFHAEVQSRVRSSLEGWMEKYAPKETFVREDATVFLVDPSDGRPAALSSEMAACL
                     KDMPQAEVFINGGYVFAIPDFLGKGRVLAESLGHDRKKLARTLAIGNDQNDIPMLDGQ
                     SAELVGCPADAAEGVIRTVRAAGGYVSSRPGPYGTLEVIEHYAGKNGKAL"
     gene            complement(20891..21889)
                     /locus_tag="PKK36_03195"
     CDS             complement(20891..21889)
                     /locus_tag="PKK36_03195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNR93597.1"
                     /translation="MKLSLPANPRILIIRMSSLGDLFHSLPVARELKKKLGATIDWVT
                     QPEYAELLECFADVDRVIVFPRKKFMQSAWWFVKSVRRNRYDAVIDLQGLGKSALAAR
                     LARARVRIGPSYHREGARFFYTHVAGRKNKNRHAVDEALDVVRYLGLEPSEIEFPVQF
                     PDVPLEGSRRVVLVPCSRWATKNWPVEKFIELGRKLTEARPDVTIFLAGSPADAGVCR
                     GIEEGMGGRVVNLCGKTSLVELGGILKSANLVITVDSGPMHMAAAIGVPVLAVFGATD
                     PVRTGPFGKPHRVVTFEGKLDCRPCLARTCRRGDLRCLSALHTDRVLSAALEMLGP"
     gene            complement(21906..22835)
                     /gene="argF"
                     /locus_tag="PKK36_03200"
     CDS             complement(21906..22835)
                     /gene="argF"
                     /locus_tag="PKK36_03200"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNR93598.1"
                     /translation="MRHFIALKDWPVEELAAMLDLAAEIKRNPGAYEDAMRRRVLVMI
                     FEKPSLRTRLSFETGMCKMGGDAIYYDMSTSPLGAGKETIEDSVKTVSRYADLVMARL
                     MKHENMLAMAKHAGIPVINGMTDYNHPCQIMADLLTISEKKGRLAGLTLSYFGDSNNN
                     VTHSILFGGALFGMHVRVGCPAGEKFSPRPDVMKEASAIAAKSGGSVAVTHDAAEAAR
                     GADVVYTDSWMSYHIPKDQLEARVKTLTPYQVNSALMKQAKPDAIFMNCLPAMRGYEQ
                     TAEVIDGPQSVVFDEAENRMHAQNAIMLKLLEG"
     gene            complement(22961..23203)
                     /locus_tag="PKK36_03205"
     CDS             complement(22961..23203)
                     /locus_tag="PKK36_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93599.1"
                     /translation="MNDLFIYGMADTADNEQWGAFSIITFFELTWYSGSIYGGIDSAH
                     RYNERRLDECLDSVSGSAEISADLNVLPLISLRFTF"
     gene            complement(23263..>24331)
                     /locus_tag="PKK36_03210"
     CDS             complement(23263..>24331)
                     /locus_tag="PKK36_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93600.1"
                     /translation="KHGPQHLYFKAKGESRDSLEDARQNALKSVQAQIAEYIFAEVEV
                     RDDAGGSRVSVESAVELRELESFKEDEAKSGGTWIVWMLGRYPRAEYDRIRKRLEMGV
                     KLESLWQKAQSALNQQKLGSAEKMLLSIIKNYDKGLRVSFDVESVKLELAGLYLKQDR
                     GLKARQFIADVQKSTTDSLWRRKADELFAQLPPVSLKDAFEGKTVGIVCFVGGSGRYE
                     CDANLASELNSRLAKYSVKTVHVKNICESVRPVIDDGFLKQIALKGRKEANVLFVAVM
                     DVDSTKTGTKIAIPGTDETTDALDAKLVYWIVRSSDGAVLASDSTVGLSSAQASLLNT
                     ILTHRRHLPQYAPGIAEQLQK"
BASE COUNT         4863 a         7541 c         6892 g         5035 t
ORIGIN      
        1 acatctgccg ctcatccgtg ccggcatcag tcgcattgaa atagaacttg aagcgtgtcg
       61 gcgctccggc cggaacgctc aatgtctggt catagctttc gatcccgtcg cgcccgttcg
      121 cccacacatg aatgctgccg gaggaggcat actcgaagtt catggtcatg gcccgatagc
      181 cgtagttggt gaacatggac gcgccattga ccgtgacggt ctggctgttg cccatattga
      241 tgttgaccag ttccgtgtcg cccgccagca gactgaagcc gcggttggat ccttcggcat
      301 tgctgtcata attgaggccc agtttgaagc tgaacacgga gcccgtcgtc attgcactgc
      361 tgaagctgcg gaggacttcc gcattggctc ccgatgcagg cggattcgcg taaaagccga
      421 aggcattctc gccaaatccc tcgatgcccg cattggtcgg gtttccgatg aagcagcctg
      481 cccagaaggt ggtcccgtcc tgcgtgtggt tgaacgacca cggcccgaag cccgacccct
      541 gattggctcc gtcggcccag ccgccggtgt agttgtcggc gctgtcctgc atggcggagg
      601 gcgctccccc tgaagtctcg cctgaaacaa ttatctggtt ggcgccgact cccagaagga
      661 tgttctcgac gctccacggt gatgcggccg cgatctgtcc cgaggcgccg gtcagcgtgt
      721 tcgtccagcg cagattcccg ttcgtcacgg cgctcgccgt gccctgcagc gtatagttcg
      781 acacggaatt ctccacggcc aaatcccccg gcgggttcgt gatcgccagt cccgcgccgc
      841 ggatcgtcac ctggaagtag agtccgccat tgttgctttc aaccgtctct tcaccgtcgg
      901 tggcctgaat aaagtagcgt acgacagtac cttcggcgaa tgtgccgaga ttcgcattgt
      961 accagctgcc gccgtcggaa ccccagtcgg cattgagcgt catggcgttg gttgcccatg
     1021 acgttccgtt ggtgctgtga acaacctgca ccccggtcac gcttccagcc ggaccggctt
     1081 cggtgtccac gtacaaatca gtcgcaaaag tcgcgtcacc gtcctcggga taatttttgg
     1141 tcccgcggat ccacaggccg gaagaggagc cctgcgcaac ggtgaaagtg tagttctggc
     1201 cgttgttgtt atcccacgcc ctgctgtcag cctcgccctg cgcttcaatg taatactcca
     1261 cgaccgtgtc ggccggttga gggccgaggg ttgcattgta ccaggcgccg ccctgactgc
     1321 tccagttggt gttcacggcc atcaccattg tggtccaggt ggtcccgccg ttcaaactgt
     1381 acccgaggag gacattggtc acgctgccgg ccggcccggc ttcagaatta atgtagattg
     1441 gagtgtcaga tgtgatttcg cccgccacgg gataggaatg tgtgccgcgg gcccacagcg
     1501 ccggcgccgc cgtgcccgcc acgaatatct tggtctcttt gcccgacagg ctgaacgaga
     1561 gccggccccc gctcgacgtg accttttccg ccggattcag gacattcgtg aactcggtgc
     1621 tgtccttgcc cacctccggg ttcagatcct tgttcccatc tccgaggttg atcgccacca
     1681 gagcctcttc gtcatcataa acccggctga aggcataggc gccgtctgtc cagcgttcct
     1741 ggaacgagcc gcgtgtcagg ctcttgtacg tctcgcgggc ggtattcagc gcggcgatgt
     1801 gcaggtagag cggcgcggtc gtggccgcca gtttgttgcc ctgcgcgggc ccgggctgga
     1861 agcccttgtc gaacatgcac tcgcgctgcc agtcgccgtc gtccgggttg tctcccgacg
     1921 gactgctccc gttgtagtgc ccgccctggt caaacacatg ctccgtgccg taatagaggc
     1981 agggcaccgg cgtagccaga tagagaaagc tcagcgccgg ccgcagcttc cagacgtcat
     2041 tcccggcgtc gccgccgttc atgagcgcaa tgcggttcag gtcgtgattg tcaatgaacg
     2101 ccaccaggcg gtcggcgcct tctccgtact ggctcttgtt gttaagctgc tgcgtaagct
     2161 gtcccgccca gccgttgtct acaaacaccc ccttgatggt ctggctcatg gggaagaaaa
     2221 gcgctgaatt gaaggaatag cccgtatcct tgcagaaact ggccagcgct ccgttgtcat
     2281 aagaaaaaag ctcgccgaag atgatgaagt cattcttgcc gcggtaggcc gcgtgcttgc
     2341 gcatgtcgtc cgcccactgc tttatccagt tgtattccac gtgcttgatg gcatcgacac
     2401 ggaatccgtc gcagtccgtc gcgtcaatca ggtttttgaa ggccttggag aggatgctct
     2461 gcaccgcggc gtcctccgtc ttcaggtcgt ccgtgccctt gaactggccg agcagatttt
     2521 ccggagagca gtcccagcag ttgatggtcc cgttgttgtg gaaatagttc aggttgtcga
     2581 acggcgcggg atgcttgttg ttctggtccc accatccaaa ggtgctgttt ccgctcgccc
     2641 agtactgttt atcttccagc tggttgttgc cccacaggcc gttcttgtcg cacatgtggt
     2701 tgggggccac atcgagaatg acatagatgc cggcggcatg gcaggcgtca atcaggtctt
     2761 tcaggtcctg aaacgtgccc atggccggat cgcacttgaa aaagttctcc ggatggtact
     2821 gatgataagg ggtgtagttc gtatccttgc cctggtcgtt catctgcaca ccggacatcc
     2881 ataccgcctt aacgcccatg ccggtcaggt atccgagatt gttcttcagg cccttccagt
     2941 cgccgccgtg gtgataattg cggttgtacg gaaaggattt gccttcgcat ttccaggccg
     3001 gcttgtagcc agccagctgg tcctcgcccg agtccgcgaa gcggtcggtg aaaagctgat
     3061 aaatattgat atcgcgccag tcagccgggc tcggggtata ggtgaagcct gattcgtcct
     3121 cccgggtttt aagaccataa acaggatcgt tcacacccca gtcaccaata ccgtcttcct
     3181 ccgcgcccgc cacgagcgga atggagagag cgcatgcgaa gaccgcaagc agagtgaaga
     3241 taagatggcg agaatcagat tttatcatgg ccgcacctgt ttcagatgtt tcaaaaaact
     3301 cggaaccttg aatagagtta accgcttgac gctgcaatgt aaagcgcttt aattcgtttt
     3361 gttttgactt ttttccacag acgtctattc tgtgttcaga tttttagcag caatactgat
     3421 ccattcaact gaatctggag gtgtttatgg caacggtgaa actgaaaggc aatccggctg
     3481 aaacttccgg gaatctcccc cggcccggtt ccgcggcccc cgctttcgaa ctcgcgaaat
     3541 ctgatctttc caatgccaga ctggccgatt tctccgggga aacccttgtt ctcaatatct
     3601 ttccaagcat tgataccccc gtctgcgcgg catcagtccg ccggtttaac gaagaagcgg
     3661 gcaaccgcaa caatgtggcg gtgctctgca tttccatgga cctccccttc gcccatgccc
     3721 gtttctgcga agtcgaaggc cttaagaatg tgactccgct gtcggcattt cgttcgccgg
     3781 cattcgggaa agactacggc ctaacgatca ccaccgggcc cctggccggc ctgctggcac
     3841 gcgcggtcat catcctggac ccgaaaggca ggatcatcta cagcgaactg gtcccggaaa
     3901 tcacgcagga gcctgactac aaggccgctc tcaacgttct tccgtagaag tcagaacttc
     3961 tgccccatat gctccagcgt caccggcgca ggattgaaca ggatcagggc ttcagcggcc
     4021 tgatctcttc cgcgaaagcg ccgagtggac actgcgggaa agagaggcca tattgaacgg
     4081 cttggcgagg aaactgatct ccgcttcgtg cccgggcgca aggttgtcaa gggttcccgc
     4141 cgcatagccg gaagtgaaaa ggacccggat gtccgggcgc agctggcgta tctgatccgc
     4201 cagttccctg ccgttcattc gcggcatcac gagatcggtg atcagcaggt caattcgccc
     4261 ctgatattcg cgaacggcct gcatggcctt gtccgggcga tcagcctcca tcacctgata
     4321 gcccagcttg cgaagcatcc gcgcgcccag tttcagtatg gtggcttcat ctgccaccag
     4381 cagcactgtc gctccgcccg tgggggccgc ctcctcgctc cgctcctttg ggccggggac
     4441 atcaatatcc ccatcacagc gggggagaca tatcctgatg cgggttccgt gacccggccg
     4501 gctgtccaca tcaatgaacc cgtggttctg gcggacaatt ccgtacacgg tggccagacc
     4561 cagtcccgtt cccctgccca cttctttggt ggtgaaaaaa ggctcaaaca gccgcgtaag
     4621 agcctccgca tccatcccgc agccgttgtc ggaaacactc aattcaacat aatcgcccgg
     4681 cgcgcattcc gggtgagccg cacaggcggc gccccgcagc ctcgcaaggc ccgtggtaat
     4741 ggtgatccgg cccgtgtccg caatggcatc ccgcgcattg acgcaaaggt tggccagcaa
     4801 atgatctatc tgggaaccat ccatcatcac cggaggaatc tgcacaccgg gccgccagac
     4861 taattcaatg ttctcgccaa tgagccgctt aagcatcttg agcagtccct caacagtcgc
     4921 attgatatcc agccggcggg gctgaatggg ctgtttgcgc gcaaacgcca gcagctgccg
     4981 ggtcaggtcg gccgaccgct ccgccgcatt gtggatttcg cggagctcat cacaaacagg
     5041 gtctgcgggg tcaagctggt ccatggccag ttccacatgg ccgaggatca gtcccagcat
     5101 attgttgaaa tcgtgggcca cgcccccggc cagacgaccg accgactcca tcttctgcgc
     5161 ctgcgccagc tgctcccgca ggcgttcgcg ctccgcttcg gcctgtttgc gctccgaaat
     5221 ctcgttgaac atcacggcgg cttcgccctc ggcaatctga aacgcataca cttcaaacgc
     5281 accccggatg ccgccatagt cgtaatccac ctgttcgctg cgccagcttt ctccgaattg
     5341 cgccgcgcgg cggtaacggt cggggatttc cgtttttgcc agcgggggga acacctcttc
     5401 gatggttttc ccgaagtaca ccgagtgatc catgccgatg atccggtctg cggcgggatt
     5461 ggccccgacc agcaccaggc ggctgtcatc ctccaggcgg taaagataaa tgcccatcgg
     5521 cgagctttcg atgacggcgc ggaaggtttc ttcgctttcc cgccccttct gctcgacacg
     5581 cttcaggtcg gccatcttct cctcaacctt gtggcggagc atcaaattaa agaccagcag
     5641 aaccgcgcag aaaccggcgg tcgccagaat ggcatagatc agcgggcgga tataggccgg
     5701 gtgactcagg gagcgtccca tccagcggtt catgacggcc cggcgctcgt cttcggtgat
     5761 ctgcgcaaac ccgtcctcca cgagctgcag cacatccaga cggccttttg ccaccgcccg
     5821 gtgaaaggcg ccggtgtaga gggtgaaaga gagcctgaag tcatcctcca ggttcatttt
     5881 gtagaggtaa tacagggccg gaggcttgtc cacggagaaa accagaatat cgcggtcaga
     5941 agcggcctga ataatggctt catagctggg atattccctg aaatccttga cccccccggc
     6001 tttcagcaca tcaatgcagg catcgccggc tttcacgcca atgggaaatc cgcgcagcga
     6061 ctcgatatcc gcaaatccgc ccagcgaatg gtgactgaac accggaacct caatatccgc
     6121 atagggacgg gtaaaatcgt aaatcgaaga ccgaactgag ttcgaaaaga tcgtgtcgag
     6181 aacatcggcg cggccaagac gcatgtattc ctgcgcaaga ttccagtcca tcgccatgag
     6241 attgacccgg acgcccgttt tctgctccca gagcttccat tcgtccggaa gaattccccg
     6301 cagaccccct ccatcatccc ggaagataaa agggggataa ttgtcatcgg aaacaaccgt
     6361 aatggtgtcg ggtgcgagcg gcccggccga tgcacagcgg atgaaaacat tccccacaaa
     6421 gaaaaagaaa aatgcgaagg ccgggccacg ctggcggcaa acacgaaaca gaacgcatac
     6481 tgctgaccgc aagcattctt tcatagacct catgccctcc gcatatcctc gacacaggcc
     6541 cctatgtctc gcagcggact tatccggttc cgccgccaca aatatcagac ctgcatgata
     6601 tccttttcct tggcagcaag gagcgcatcg attttcttaa tgtagtcgtc ggtgtatttc
     6661 tggatttcct ccagacccga atcacgctca tcctctgtga tcttgctgct tttctgcaga
     6721 ttcttaatgg cctcattggc atcacggcgg acattgcgca cggcaacgcg ctgctcctcc
     6781 gcctggcgct tggccacttt ggtgagttcc cggcggcgct cctcggtcag ttccggaatc
     6841 ggaatgcgga tcagacggcc gtcgctgatc ggcgtgacgc cgatgttggc ggccagaata
     6901 gctttttcga ttgcgggcag agcggatggg tcgtaggcgt tgatcacaat gaggcgcggt
     6961 tcgggcgttg agatgccggc gatttcccgc aggcgcgtgg cggcgccgta gtagtccacg
     7021 gcgatgtgat ccacgaggct tggcgacgcc ttgccggtgc gcagccccgc aagctcctgc
     7081 tgcaggtact ccagcgcctt gctcatcttg tcgtcgctgt cgagcagtat gtcgtcgagc
     7141 gtttccatgc ttttcctccc ttgcgctact gatgcaccac ggtgccgaca ggctcgccct
     7201 tcagcacgcg cacaatttca tcatccttga accagttgaa cacaataatc ggaatgtcat
     7261 tttccatgca cagcgaaaag gccgacgaat ccatgacttt gaggcgtttg ttcagcgcct
     7321 ccttgtagga gatatcgtcg tagcgcacgg cggagggatc tttgacggga tcggctgtat
     7381 aaatgccgtc caccttggtc cccttcagca gggcgccggc cttgatttcg caggcgcgca
     7441 gcgcggcggc gctgtcggtt gtgaaatagg ggtttccggt tccgcccccg atgatcacaa
     7501 tgcggccctt ttccagatgg ccaacggcct tggcggcaat cgccgcctcg gcgatgcggg
     7561 gcatttcgac gcccgacatc acgcaggttt ccaccccgag gttttcgagc gcggactgca
     7621 gggcaagggc gttgatcatg gtggccagca tgcccatgtt gtcgccccgg gcgcggtcaa
     7681 tgccgccctc aaggcccgaa agtccgcgga agatgttgcc gccgccgttc acaacggcca
     7741 gttgaatgcc cgcgtcatgc acttttttaa gctgtcgggc cgccttctgg agcatttctg
     7801 gagacaggct ttcgcttttg tccccgtcca ggaatgcttc tccgctcagt ttaagcagaa
     7861 cccgtttgta cttcatttga tcttttccag atgccatctt gatctttcgc tgataattac
     7921 tagattaaac gctcatgata aatgacggaa cgcgggcgga gcaaggtaaa aacctcaccc
     7981 ggttgcaaaa acccgtgccg tcctgtatgc ttttagacgg agagatggaa attggacaga
     8041 aaagacttat cgctgatcac tgaaggatgg gattacaacc cccgcgaagt tcgcgcgcgc
     8101 tgggttaaaa ccgcgtcggg cgaggcggaa gtgcagcttc ggcttgattt gggcgtgctg
     8161 cagatgcgcg cggagggccg gcccgacggc cagacgccgc gcggatttcc ttcgcttctg
     8221 gactattatg aggaactgga acgcaccgtg ccggaggatc atccctcgct tcagtttgac
     8281 gaggacatgt gcgcagaact tcagcaggag gcggtccagt actactatcg ctacctggcc
     8341 tattccgcgc tgggccacct cgacggggtc atcgcggaca cagaccacga cctgtccatt
     8401 ttcgaccttg tggccgaaca cgccattgat gatgatgtgg cgtggcagtt cattcagttc
     8461 ttcccctatg tccgcatgat gaacagcaag gcgctggccg aaaaggggct gcgcgagaag
     8521 aactatgacg aagccctggc cgcggtggag gaaggcctct gcgacatccg caatttcatg
     8581 caggactata cggacgagga cgaggatttc gaggactgtc cggaaatccg caccctgcag
     8641 tcgctgcagc acaagatcat acggatgcag ccgcggagcg aagaggatcg cattcacgag
     8701 gaactcgcgg cctccatcgc cagcgagaac tacgagcacg ccgcccgcct gagggatcgg
     8761 ctcaagaagc tcgggaagac ccccttctga cgcacggcga aaggccggga aaaaaggaaa
     8821 cctgatgaaa aagcagatcc cttgggaact gtacagccgg attattcaga acgtgccgat
     8881 cgcatgcgtg gatgtggcca ttgtcgccaa tggcgcggtc ctgctggtgc ggcggaagga
     8941 cgaccccgca aaaggccagt ggtggcttcc gggcggacgg gtgctcaagg gggaaatgat
     9001 gaaggccgcc gcggcgcgca aagcgcgcga tgaagtgggc atcgagtgcc atgtgggacc
     9061 cattgttcat acagcggaaa caatctttcc cgacgggccg ggcggcatca gcatccacag
     9121 catcaacagc tgtttcttca tgtatcccgt gaaggcagac ttcaaaccgt cgctggacag
     9181 ccatcacaca gacgcaaaat gggtccgaag gattcccaaa ggcgttcatc cctatgtgga
     9241 acagtgcctc atgggcgccg gcctctcaaa atgagtccgc gcagagccgc gcaggcgggc
     9301 gttccggtct tcttcaccac aggttgttgt gaatgcggga gggatcaagg cggtcctctc
     9361 cctgaaggtt ttcattcggg tggccggcag cgatcagcgc gaacgggaaa atgttttccg
     9421 gcacattcag aatctcgcgg cagggattga agcggtcggg aatgggatga attgcgatcc
     9481 acacggcccc gaggcccagg gcatgggccg ccagcagaat gttttcggcg caggccgagc
     9541 agtcctgcac ccagtagccc ggaattttct cgagcgcttc gtcgccacag accagaatgc
     9601 ccagcgtggc cgtgtccagc atttcgcatt tgggcatggc ggcggaaagt ttccggagaa
     9661 cagcggtgtc ggttatgaca acaaaatgcc acggacgctg atcgccggcc gaaggggcca
     9721 cgctggccgc cttcatcaga atttcaatct gatcccccgc caccggctga cgggtgaact
     9781 tgcgaatgct tcgccttgaa aatattgcct gcagtgcgtc cataaccgta atctccttcc
     9841 aaatcgaggc cattatgaaa gtcgaccggg aatcaggcaa tcacattctc gcacagcttt
     9901 tcgcggaggc ctcctggatg acactgcagg aaatgcttgc gcaaaggcgg ggtcatgcta
     9961 aactgaatcc tgcatgatta acaattacag gaggagatca tgaagttata tcaggtattg
    10021 agcattctgg ctgttgtttc tgttgcactg gctgccggct gcagcaaggg cacgacagaa
    10081 caggcggcgc ccgcagcggg cgtggcggca gcttctgacg aaggattcgt cgcaattgac
    10141 tccagtctgc tggagaaggt gaaatatgac ggcgcagccc agacgctgac ggttgtgttt
    10201 gacagcggtg aaacgtatgt ctacgccggc gtgccccagg cgatctatga cggcctgatg
    10261 gccgcgccct caaagggttc gttcttcgaa gccagcatca aggacgcctt ccagttcgaa
    10321 aagaaataat tcgcggggct ttccacatgc gcacccggag caaatgctcc gggtgttttt
    10381 ttatgcggtt gcaatccgcg ggcctctgcc cttaaaatgc accgcgtgat gtgaggagaa
    10441 cttatgcagt cagactggct ttctgttctt gcggccgcgg cgggatcata tctgctgggg
    10501 tcagtcccct tcgggctgct gctggcccgt gccaggggag ttgacatccg ctccgtgggc
    10561 agcgggaaca tcggcgccac caatgtgttc cgggccgtcg ggaaaggtcc cggcctcctg
    10621 acttttttcc tcgatttcct caaggggctc ctccccgtgc tggcggccgg acactggctg
    10681 gctccggaca cagccgcgcc gattctctgc ggctgcgcgg caattgccgg gcatatatgg
    10741 actgttttcg cgcaattcag gggcgggaaa ggcgtcgcca cgggcgcggg agtcgtggtg
    10801 ggcattgcat gggaggctgt tattatcggc ctgatctgct ggatcatcgt ctttgccctt
    10861 acccgttatg tttcactggc ctccattatc acggctggtt cggtcggaat atccggatgg
    10921 attctctatc ccggccgccg gctggttccc gcgacactga ccctgctggc cgcactggtc
    10981 attgtgcggc accgttcaaa catcgtccgg ctgctcaagg gaaccgaaaa tcgctttgac
    11041 ttcaaaaaga aaaaggaagg cgcagaatga aaagagttgc ggtaatcggt gacggaggct
    11101 ggggcaccgc gctggccacg gttctgcagg ccaacgggca ccacgtcaca gtctggggtc
    11161 cttttgcaga ttacatcgga gaaatcatgg ccgcccgcga gaaccgcaaa ttcctccccg
    11221 gcgtaaaact tcctccggag attcaatgga cagcagaccg ggcggaggcg gcgaaaaacg
    11281 cagatgcctg ggtcctggct gtgccatcga agttctatga agaagtggtt gcgtcctttc
    11341 gtccgtttgc agataaaaac acacacgcca tcagcgtgac caagggactg gaccggaaaa
    11401 cgcaccggcg catgagtgaa atggccgcgg atctgcttcg gggaggcccc attgtcgccc
    11461 tcaccggtcc aagccatgcg gaagaagtcg cccgcggcat tccaaccgcc gtggtcgccg
    11521 cctgcgagga tcacgcgcgc gccgagtttg tgcaggaact gttcagcaac ccgcgcttcc
    11581 gcgtgtacac gacagacgac accgtcggcg ccgaactggg cggctgtctc aagaatgtga
    11641 tcgccatcgc cgtcggggtc agcgacggga tcggcttcgg ggacaacacc cgcgccgcgc
    11701 tgatcacccg cggactggcc gaaatgaagc gtcttggatg cgcactgggg gcacatgccg
    11761 aaaccttttc cgggctgagc ggcatgggcg acctgatcgt cacctgcacc agcaggctca
    11821 gccgcaaccg ggcggtaggc gaaaaactgg gccgcggcca gtccattgaa tccattcttg
    11881 ccggaatgga acaggtggcc gaaggcgtgg caaacagcgc ctcggccctc gcgctggccg
    11941 aagcacacgg cgtggaagtt cccatcacgc aggaggtgaa cgacgtgatt cacaggggca
    12001 aaaatcccaa gcttgccgtg caggccctgc tgacgcgtga tccgcggccc gagagagatt
    12061 ccccgtgaaa atccacctcg ggattgtgct ggcggccggg gcatcgtccc ggatgggctc
    12121 cccgaaggcg ctcctgcggc cgccggacgg cattcccctc gccctccatc agatccggct
    12181 gctagaatcg gggggatgct ccaaaaccgc cattgtactc ggagcggagg ccgaccgcat
    12241 tgcgccggaa ctcccgggag tccgcatcat cctgaatccc gcgtgggaat ccgggcggcc
    12301 gtcgtccgtt ctggccgcgc tgaagggcgc ggcggattgc gaaggcatga tcatcctgcc
    12361 ggtggacacg gtcggcatcc ggcccgaaac cataaaacgt gttctggaat tttccgatac
    12421 ggctgaatgg ccggcggtgc gtccggtctg caggggaatt cgcggcaaac tggtgtggat
    12481 ttcgtccgcg ctttcagatg aaatcataca gaagaactat tccgaaaggc tggacgacct
    12541 ccttgcggac cgggccttcg aactggaagt ggacgatccg gccattttgc ggaacatcaa
    12601 cacgccaaaa gactgggaag aagtctccgg acagctctga aatcaggatt cgagcaggaa
    12661 acgcaccagc cggcccgaaa cccatttccg atattcggcg gtggaacgaa tgtcgtctat
    12721 cggagatact tcgtccgacg cgcgcttctc aacctccgcc agcgtttcct cactcgcgga
    12781 ttttccgatg atccattcct ccagtttcgg cagacggacg gtcgttgggc ccacgctgcc
    12841 cagcgcgacg gcgcaggttt cgaacgatcc ggccttcacc gagaaccggg ccgcgcacat
    12901 caccttggag atcgcctgcg cggcgcgcgt gccgagcttg cggaacacct caatctctcc
    12961 ggcggcacgc ttcgggagta caaaacgcac gatcatttca tcgggtttca ggtcaatcct
    13021 gcggtaggcg aggaaaaatt tcgtcagcgg gatcgtgcgc tctccggtcc ggctcgccac
    13081 gacggcctct ccgccggtga tcagcagcgc aggagccaga tcgcccgccg ggctggcgtt
    13141 ggccacattg ccgccaatgg tgccaagcgc ctgaatctgg ggcccgccca ctgtggccgc
    13201 ggcggccgcc agggccggca gatgcttctg caccggtttg gaactccgaa tgtgcgcgtg
    13261 cgtcacgccg gcgccaatga caacggcgcc gtcatcctct ttaatcccct gcagttcctt
    13321 cagccccttg atgctgatca cgcgcggagg cggctccatg cctgccgccc actgaaccat
    13381 caaatcgctt cctcccgcca gaagccggcc gcgggtggac tcgttcgcca ggagctccag
    13441 cgcctctttg agcgtggaag ggaaacggca gtctgatttt tccagctttt tcataatcac
    13501 ttccggggcc agttttgcgc cgccagcctg acagcttcaa tgattttcac ataacccgtg
    13561 cagcggcaga tattgccgcc atgagcatcc agaatgtctt cttctgtcgg ccggctgttt
    13621 ttcgagagaa gcgcgaagga cgccatgagc atgccgggga tgcagaatcc gcactgagat
    13681 gcgcccgccg aaacataagc ctcctgaagc acccggccct gctcggtttt ctccagccct
    13741 tccacggtca gaatctgcgt tccatcgggc acctgccccg cggccaccag acaggaggtc
    13801 accggcacgc cgtccagcag caccgtgcag gcgccgcact ctccctcgcc gcagccttcc
    13861 ttgcatccgg tgaaccgcat atcctcgcgg atcacatcca gcagccgacg cagcggagga
    13921 aactccagcg tcttctcctc gccattcacg ttccaggttc ttttcacgtt gctcatagtc
    13981 tatccattct gatgctttgc gcggtgcagc gtgaacagat cctccggcat gatgggaatc
    14041 cggttgcacc gcagccccgt ggcgttctca atggcgttgg cgatggcagg agccagcccg
    14101 tccatcggca tctccccgat gcctttcgcg ccgggcggag aaaattcgta gggatattca
    14161 acaaaggtga tgtcgaattc gggaatgtcc ggcatggtcg gcacaatgta ggtctgcatg
    14221 cggtcggcat catatttccc gttgcggatg cccatcttct ccatcagcgc atagccaatg
    14281 gtctgcacca gcccgccttc cgcctgcgcc ttgcagttga gcggattaat catgcgcccg
    14341 acgtcgaaac aggccgtcac tttcttgagc cggatttcaa aggtcagggg atccacctcc
    14401 acctcggccg cattgcatcc ccaggaaaac gccggatatg cgtctccctt gaacgtgctc
    14461 tggtcccact tcagatcagg cgggagcgtg aactgatgct ccgtccgcag acggccgtgc
    14521 ttgtcaagat acgaggcggc aatctgccgg aagggcagcg cctcgccgct gcagacgaac
    14581 cgcccgtttt ccaggacaac cggccggcct tcaaaaacct cttttgaggc gaactcctcc
    14641 agcgccgcct tcagcttcag gcccgcgcca tgaacggtat gcccgacgac catggtcgtg
    14701 cgcgaggcca ccgtcggccc gctgttgggg cacaaagaag tgtcgggaag cggacagcga
    14761 acatcgtcaa ggggtatgga aatcgcgtca gccgcgatct gatccatgat cgtgaaagcg
    14821 ccctgcccca tttctgtcga gctgatgcgt atgttcaccc cgggatggtt tggttcaatc
    14881 cagtccagct caacggcgcc ctttgccttg aagcgcgcct cgccgtcgcc ggtgaagccg
    14941 gatccgtggg cgaaaaatgc caggccgatg ccgtaccact tcttgtgttt tggattaccc
    15001 cggctggact gcttcagttt ctttgcaaaa tccgaccggt ccagcacctg atccagaacg
    15061 gctggagacc ccacactgtc tttcagcacc atgcccgtgg gggtgacatc cccctgcaca
    15121 agacagttct tccggcggaa ttcctgcggg agcatgccgc aggctttcgc cagctcctcg
    15181 atatgcgatt caaaagccca ggtcgcctgc ggggcgccga agccgcgaaa agcgccgcag
    15241 gggaacgtgt gcgtcttgaa aatgcggccg tccacaaaaa cattgtcgca ccggtagccc
    15301 atggccgcat gcagaatacc gcgcgcgaga accacccggc tgatcgtcgt ataggcgccg
    15361 ccgttcagca gcagttccgc gtgaatcgct gtgatcgtgc cgtccttctt cagtccggtc
    15421 ttcaggcggc cccaggacgg atgacgcttg gtcgtgaaca ggatgtcctc gttccggtcg
    15481 aaaacaattt tcaccggatg acgcgacttc atggccagca gcgcacagta gccggcaatc
    15541 agcgtgggat actcctcctt gccgccgaac gcgccgccgg tcatctcctg cttcacgcgg
    15601 attttctcgg gcggcagatt gagcgtcagg cacaactccg gattgatgaa atagggacac
    15661 tgcagactgc cgcggatgaa tacccctccg tcgtccatca tttcggcgat catggcctgc
    15721 ggctcaatat aaagctgctc ctgataaccc gcccagtact cgccggtgac aatcatatcc
    15781 gcttctgaaa aacccttttc gatgtcaccc ttgaccgtgg tctgctcgcc gagcgaatga
    15841 agcgaggaat cctgtttctt gtacaggtcc acaatttcct ccatgtggaa gatgcccgga
    15901 agctcctcaa tgtcgggatg gaaatggcgg gcggcctcct cggccagctc acggtcggga
    15961 gcggccacca gcgccagcgg ctcccccaga taattgattt catcgtgcgc gatgaagggc
    16021 atgtcatgga tgaaaaggtc cgcgatgttc ttgccgggga tgtcatccgg gaggaccacg
    16081 ctgacccggc tccagtcaaa ttcctcatcc cttttcaggg agcgcaggcg gccgtgtgcc
    16141 accggtgaac gaacgacttt cccgtaccag cagcccggca cgtcaatatc gtccgtgtaa
    16201 accgcgcggc cccggacctt gtcgggcgca tccacccgcg tgctgtcagc gcccacgtgt
    16261 cctgatttga ttttgcgttt tgggcccatg ctccgtgctc ctgaaaatag tcctgcaaag
    16321 actataaacg aatccggatc aaacgcaaaa agaaatgccc gggcgcgcgc aaaaaaccgc
    16381 cggcccccct gcggggccgg cggcctgcga tacaatgacg ataatttatt ccggattcac
    16441 gccgatgcgg ataatcctgt gggcggcggc atcaccgagg atggtgacct gtccgccgga
    16501 caccgtatag tctgtgttct cgaccagcag ttcccagtcc cagcttccat cctccagaac
    16561 ggtgtcggcg ccgtaaactg ctcccagagt gtatccggcc ggtacgtcga aggtgaaaga
    16621 actgccgccc gctccgggaa caatctcgcc gatctcggga ggaccggcag gcgctttggt
    16681 cacatacatg cggttgaagt agaagttctc ctcgtccccg cttcctccgt tgtagttgta
    16741 gaattcgatc tggtcaatcg tgccgggcag caccacatcc tccaccagca ccggcgtctc
    16801 ttcggcgccg cgcaggaaag agaaggtgaa ggtgttcggg gccgtgcaga tggcctgcac
    16861 ccggcccatg aagcctccgg ccagataatt ggaccagatc accacggggg ttccggcatc
    16921 cagcttgtac gaccaggtgt caccgccgct gttgtaaagc tcgaaccgct cgtacggagt
    16981 accctcaaaa tcgcccttca gcttgaagcc ctttgtgccg ccgctgtagt tgtaggccac
    17041 gtcaacagag aacgtctgcc cggacaccat cggcgcgggg aactcgcgcg aaactttgat
    17101 ctccggcaga ggatcgggct gggctccggt ataattcgca tacagtccga acgccgtacc
    17161 gccttcatta atgccgccga tggcggaggc cccgatgaac tttccgcgat aggaatcgtt
    17221 tgtctccggg ctgagcgttt ccgtccagtc ggaaaagccc gtgacatgag ccggcgagtt
    17281 ggaccagacc ccgccctcgt acagcgagga atcgtcatac gcgtcaaact ccggccgttc
    17341 ggtcacggtg acatccagat aggtgctggc gtccccgccg tcgttggcgg tcaggcgggt
    17401 ctgccccgcc gcaagaaccg tggcctcaaa ggtgaggctg ttcgaccccg actggaaatc
    17461 aaccgaagca ggcacggtca tcaccgcggt gttggtgctg cccaggaata ttgtgtcggg
    17521 cacggcaccc ttgcgcgtta tggtataggt ttccgttgtt ccgccggtcc atatgtcatc
    17581 acggcccgtg atggtcagtt ccggggcctg cacggtgaca tcatacgttg caaaggcgcc
    17641 gctggccgcg ttgctggcgg tcagcgtggc ggttccaaac cataccggag tggcttcaaa
    17701 caccaggctg gtgcttccgc ccccgaaggc tgcgctgttg gtcacggtca gcacgccctc
    17761 gtcggaactg ctcaggatga tatcgtcacc cacgccgcct tcgcgcgtca gggtgtactc
    17821 cgccgggatc cccggtaccg tcagttccca tggtccggcg atgtaaagaa ccggaagcgc
    17881 gggcttcact tcatattccg cccatgcgcc ggtctcttcg ttgctggcaa tgagcgtcgc
    17941 ttcgccggcc gtcaggctga cgaccgtggc caggaagctc acggtgttgg acccttcttc
    18001 aaattgcaca ctcccaggaa ccgtgacggc attggtgttg ctgctgctga gcacgatgtt
    18061 gctcccgacc gggccgctgc gctcgatcac gaattcataa tcgcccagcg cgccgggatt
    18121 ccatgtgccg tcggaaaaca ccaggctcac gcgctcatct cccgcgatct gcaggttgtt
    18181 catgaagatc gcgtagttgg cattgtcccc tatcggatcg gccgtgtagc cgccgcaata
    18241 catggccacg cttgtcgcgg gttcgccgtg cacaatattc gttgtgtagg ccagattgac
    18301 tccgccggtc accgaccggg tcagatacac ctcaatcccg ccggccagct gggttatttc
    18361 cacattcacc acggcgcccg gcgaatatgc agtcgaaatg atctgctgat tgaccttgaa
    18421 cacattgccc ggagttacat caatcaggtt ggcaaactgc ccgctctggg aggaaatatc
    18481 cacgccgcgg ccgccgccgt cccagttgta ggtgaacgtg aagctgagga cattgccaac
    18541 ccgcagcgcg ccgccgaggt tgcggctggc gttgcaccat ccgttggacc cgtcgcccat
    18601 gaaacggaag gaatacccgt tggtggaatt caggtctccg ccgccgcccg ccgtggagtc
    18661 tccgagttcc gcatccttgt tccagaaatt ccaggggccc agacccagac cttcatttga
    18721 accgttggtg aaagttgccg gcgtgtagtt gcccgcctcg tcatgggcga tgacgttcgg
    18781 cgtttccgtg accgtgacct caaagggagc actcgcggcc gaagcgtttg tggctgtaat
    18841 ctgcgccgcg cccgccatgc cgatcacggc ggggaagctc acagagttct ggccggacgg
    18901 gaaatccaca gtggcgggaa cggacaggac attcgtgttg gagctggaca ggttcacggt
    18961 gtcgtccacc atgccgaggc gcgtgacggt gtaccatttc gtgctgttgg tccaggccgc
    19021 ggcggggccg ttcacctgaa gcgtcggaac ctgaatgttg acgacatacg tcgctgaagc
    19081 gccggtggcc gagttggtgg cggtgatggt cgctgcgccg ttggtcacac tgaccaccgc
    19141 tgccgtaaag gtcagactgt ccacgcccgt ttcaaaagac accgatgcgg gcaccgtcag
    19201 cgcatcctcg ttgtcgctgg aaaggtctat gtcatcgccc acctcgccga tgcggcccag
    19261 cgtgtattca tagtctccca gcgcgccggg attggcgtcg cccgcagtga aggtaagaat
    19321 gagaggatcc ggcggaggcg tgccttcctg attaagctcc agattgtcga aatagctctg
    19381 ccgctggtct tccagcgtgt ccggaaggcc cgaagaatag aagcgcatgc ttccaagata
    19441 cccgtagcca ttggccacag ttaccgttgc cgagaatgtt tccgagccgt cccggcctgt
    19501 tgcctgcacg gcgatctggt cagccgcttc ctgcgtgaac gtccagcgca tggtgttggt
    19561 gccgaattct gccaaggcga cattcccgtt catgtagatc tcgccgggaa aacttccgtg
    19621 gttcacattg atcacttctt catccagcgc atacaggctg aaccccttgt tcccgccgga
    19681 ggaatcccag ttcactccga aatcgagcgc gaaggtgtcg cccgtgttaa gggcctgcga
    19741 gaaatcgcgg tcaatattga tgtagccaac cttgcccaca tagccaaagg ccaaccccat
    19801 gttcaggccg gcaccggcac tgtcccagat gccatgtccg gcgaatccgc cctgtgcatc
    19861 cgcgacaaca ctccacgggc cgaaaccggt gccgccgttg gattcattgg tccagccgcc
    19921 gggataattg gcggcggaat ccgtataggt tgcccccatg agatttcctg caaacgccag
    19981 cgctgcagtc aggacagaca tcagtgtgta cttagtcatg atcagtctcc tcgtttggat
    20041 gattctgcag gactatacgc tgaaaaccgg ggccagtgca agaaaaatta aagcgcttta
    20101 ccgtttttac ccgcgtagtg ctctatcacc tccaaagtgc cgtatggacc cggccgggac
    20161 gacacataac ctccggcggc acgcaccgtg cgaatgaccc cctccgcggc atccgccgga
    20221 caccccacca gctctgccga ctggccgtcc agcatcggaa tgtcgttctg atcgttcccg
    20281 atcgccagcg ttcgagccag cttcttccgg tcatgcccca gactctccgc aagcacccgg
    20341 cctttgccca gaaaatcggg aatggcaaac acatagcccc cgttgatgaa tacttccgcc
    20401 tgcggcatgt ccttgagaca cgccgccatc tcggacgaca gcgccgcggg acggccatcg
    20461 gacggatcaa ccaggaaaac cgtcgcgtcc tcacgcacaa aggtctcctt cggagcgtat
    20521 ttctccatcc agccctcaag tgaactccgc acccgcgact gaacctccgc atggaaccgg
    20581 cgcagatacg cccgcgtgtc tgtgttccat ggttcacggt cggcatagcc ggagccggaa
    20641 cggtcatata tgaaactctc gccgcagatc atcttttccg gcctgtgaac cagtccgcgc
    20701 ttcacggaaa gatcaagcac ctcaagctgg ttcctgatgc cccgaccgct gttggtgaac
    20761 cactgaatcc ccgccgcctc aagcgcattc aagcattcga tcaccgccgg atggaaccat
    20821 ccaagttcgc cctcgtactc catgattgtg ccgtcgaaat caaggcagac acagtacagc
    20881 gacgcctgcg tcatggtccc agcatctcca gcgccgcgga cagcacacgg tccgtgtgca
    20941 gcgcggacag gcagcgcagg tcgccgcggc ggcaggtgcg cgcaaggcag gggcggcagt
    21001 ccagttttcc ttcgaaggtc accacgcggt gcggctttcc aaaaggcccc gtgcggaccg
    21061 gatcggtggc cccgaacacc gccagcacgg gcacgccgat cgcggcggcc atatgcatgg
    21121 gcccggagtc aaccgtaatc accagattcg ccgacttcag gatgccgccg agttcaacca
    21181 gtgaagtttt accgcagaga ttcacaaccc ggccgcccat gccctcttca atcccgcggc
    21241 acaccccggc atccgcaggc gatccggcaa ggaatatcgt cacatccggc cgggcttccg
    21301 tcagcttgcg ccccagttca atgaacttct caaccggcca gtttttggtc gcccatcgcg
    21361 aacagggcac cagaaccacc ctccggctgc cctccagcgg cacatcgggg aactgcacag
    21421 ggaattcgat ttccgaaggc tcgagtccca gatagcgcac cacatccagc gcttcgtcca
    21481 ccgcgtggcg gtttttgttc ttcctgccgg ccacatgcgt gtagaaaaac cgcgcgccct
    21541 cgcgatgata cgaagggcct atccggacgc gggcccgcgc cagccgcgcc gcaagggcgc
    21601 tcttgcccag cccctgaaga tcaatcaccg cgtcatagcg gttcctgcgg accgatttca
    21661 caaaccacca ggccgactgc atgaacttct tccgggggaa aacaatcaca cgatccacat
    21721 ccgcgaagca ctccagcagt tcggcgtact ccggctgcgt cacccagtct atcgtcgcgc
    21781 cgagcttctt tttcagctcg cgcgccaccg gcagcgagtg aaacagatcg cccagcgaac
    21841 tcatcctgat gatcaggatt ctcggattgg ccggcagtga aagtttcatg cgcgcctgcg
    21901 ggaattcagc cttccagaag tttcagcatg atcgcgttct gggcatgcat ccggttttcg
    21961 gcctcgtcaa acaccaccga ctgcggcccg tcaatcacct cggccgtctg ctcgtaaccg
    22021 cgcatcgccg gcaggcagtt catgaagatg gcgtcaggct tcgcctgctt catcaaagcc
    22081 gaattcacct gatagggcgt cagggtcttc acgcgcgcct caagctggtc cttgggaatg
    22141 tgatagctca tccacgaatc ggtataaacc acatccgccc cgcgcgcggc ctcggcggcg
    22201 tcgtgcgtga ccgccacgct gccgccgctc ttcgcggcga tcgcggaagc ctctttcatc
    22261 acatccggac gcggagaaaa tttttcgccg gccgggcagc ccacgcgcac atgcatgccg
    22321 aagagcgctc cgccgaacag aatcgaatgc gtcacgttgt tgttggagtc tccgaagtag
    22381 ctcagggtca gtcccgccag ccggcccttc ttctcgctga tcgtcagcag gtcggccatg
    22441 atctggcacg ggtgattgta gtcggtcatg ccgttgatca ccgggatgcc cgcatgcttt
    22501 gccatggcca gcatgttctc atgcttcatc aggcgggcca tgaccagatc ggcgtagcgg
    22561 ctgacggtct tcacgctgtc ctcgatcgtc tccttgcccg cgcccagcgg cgaagtggac
    22621 atgtcgtaat aaatcgcatc gccgcccatc ttgcacatgc cggtctcgaa cgaaagacgc
    22681 gtgcgcaggc tgggcttctc gaagatcatc accagcaccc ggcggcgcat ggcatcctcg
    22741 tacgcgccgg gattcctctt gatctcggcc gccagatcca gcatcgcggc cagctcctca
    22801 accggccagt ccttcaacgc aataaaatga cgcatgacaa actccttccc ttcaaaaaga
    22861 catttggaaa agtcctgaac ggtataacaa agcccccgcc ctctggccag tcagaatctg
    22921 agatttcctc ggccccggtc aggagcagac ccgggatgga tcagaatgtg aaccggagcg
    22981 aaatcagcgg caggacattc agatcagccg aaatctccgc cgatccgctg acggagtcca
    23041 ggcattcgtc cagccttctc tcattgtaac gatgggccga atctatccct ccgtaaatgc
    23101 ttccgctgta ccaggtcagt tcaaagaagg tgatgatgga aaaagcgccc cactgctcat
    23161 tatccgcggt gtcggccatt ccatagatga aaaggtcatt caagatccat gaacttatca
    23221 atatgaaacc ggccgcttga tgcgagcaag cgtcaccccg cctcacttct gcaactgttc
    23281 agcaatcccc ggagcatatt ggggaagatg ccggcgatgg gtcagaatgg tgttcagcag
    23341 gctggcttgt gcgctcgaca ggccaacggt actgtctgaa gcaagaaccg cgccatctga
    23401 cgatctcact atccaataga ccagtttggc atcaagggcg tctgttgtct catccgtgcc
    23461 ggggatagca atctttgtcc cggttttagt tgaatccacg tccatcactg ctacaaaaag
    23521 aacattcgct tctttccggc cctttagcgc gatttgtttc agaaatccgt catctatcac
    23581 cggccgaacg gattcgcata tattcttcac atgaacggtt ttcacagaat atttcgccag
    23641 acgactgttc aactccgacg caagattcgc atcacattca taacgtccac tgccgcccac
    23701 aaaacacaca atacccacag ttttaccctc aaaagcatct ttcagggaaa ccggcggcag
    23761 ttgggcgaag agttcatcgg ccttccttcg ccataaggag tccgtcgtgc ttttctgcac
    23821 gtccgcaatg aactgcctgg ctttaagacc tctatcttgt ttcaggtaaa gcccggccag
    23881 ctccagctta acggattcga catcaaacga cacgcgcagg cccttgtcgt agttctttat
    23941 tatgcttaaa agcatttttt cggcactgcc cagtttctgc tgattcagtg cggactgggc
    24001 tttctgccac aggctttcaa gctttacccc catttcaagc ctcttgcgaa tacgatcata
    24061 ttccgcacgg gggtaccggc caagcatcca tacaatccag gttccgccgg attttgcctc
    24121 atcctccttg aaggattcca actcgcgcag ctcaacagca ctttcgacgg acacacgaga
    24181 tccccccgca tcatcacgga cctcaacctc ggcaaaaata tattccgcga tttgggcttg
    24241 aactgacttc agcgcattct gccgcgcatc ttccagagaa tccctgcttt cccccttggc
    24301 tttgaaataa agatgctgcg gaccgtgctt c
//
LOCUS       DAOQWR010000022        23382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2539_length_23382_cov_5.868950, whole genome shotgun sequence.
ACCESSION   DAOQWR010000022 DAOQWR010000000
VERSION     DAOQWR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23382
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_23382_cov_5.868950"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PKK36_03215"
     CDS             <1..362
                     /locus_tag="PKK36_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNR93601.1"
                     /translation="MFSSTLGEYSIFEEAFKGLYLAIGAFFGLILFGGMSLLGAPVMM
                     TYGVIRGLGQTMPHSVIPQFIGALIGRYYFQKRLGLIWRQYIPVVSAGFACGMGLITT
                     VGVGITFLSKAAIPLPF"
     gene            371..1093
                     /locus_tag="PKK36_03220"
     CDS             371..1093
                     /locus_tag="PKK36_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93602.1"
                     /translation="MAEVLIEIRGVNKIFDLGKVKVHALKDVDLDVYRGEYLSIMGPS
                     GSGKSTLFNMIGALDQPTSGTIRVADISLTDLSSRELAYFRGKHIGYIFQTFNLLPAY
                     NALENVAMPLVFNGWEEARAHARAAEVLEEVGLGHRITHRPEELSGGQQQRVAIARAL
                     ANKPAIILADEPTGNLDLHTGEEIIRLLSQLSRELGVTVISATHDHKMLATSDRILWI
                     RDGRKDRLEKREDIRIRIGTVS"
     gene            complement(1100..1648)
                     /locus_tag="PKK36_03225"
     CDS             complement(1100..1648)
                     /locus_tag="PKK36_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNR93603.1"
                     /translation="MPDSDAPAPERKWVCDLVLPEKPLRWAVLHTRPRCEKKLCRYCE
                     EQHIPVHLPQIRKLHRYGARVREFENPLFPGYVFCILRPEQDAGLKQSQYVANLLQVF
                     EQERLVRELKQIETALKAEKVVEVLPYLKKGSLVRIKSGQFKDFEGVVEHMHGKTRLV
                     INITMINYSMAIEVDAAFLAPA"
     gene            complement(1641..3074)
                     /locus_tag="PKK36_03230"
     CDS             complement(1641..3074)
                     /locus_tag="PKK36_03230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNR93604.1"
                     /translation="MALFVSDVLTIVLAVFLSAIMRLGWKIGLEYLGDNLLPLTSVSF
                     VFLLIFYAGGMYEREVITQKLGASFFLPVVVTALGLVVTILAFYADFELHIGRGVLLL
                     ASLFIAVGSWVARRLLRMALGYGLFSKKTLILGGGREAEEVLALLAAEPNSGYSLYGI
                     VSVEPGDAEAFVRGVPVLGDIASLREYVAAYDVETLVVATPPDKAHTLLHVLRPLRYA
                     GLQILDYVSLYEELGAEIPIDHIDDEWLMHAAMNSSRIHIRQIKRVMDLSASVIGLIV
                     LVPVGVIAAILIKLTSPGPVFFRQKRVGREGRVYTLLKLRTMRQDAEAKTGAVWATAG
                     DRRITPVGRFLRKWRIDEIPQLINVLRGEMSLVGPRPERPEFVALLKQSIPFYQERLM
                     VAPGITGWAQVKFPYATSIEATKRKLQFDLYYIKHMSLYLDVLILLRTFRTIIVGLRY
                     AEEPENGSVPQTAASPAGPASGETANA"
     gene            complement(3155..5392)
                     /locus_tag="PKK36_03235"
     CDS             complement(3155..5392)
                     /locus_tag="PKK36_03235"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNR93605.1"
                     /translation="MKGIIEPPSPPHAAGQVPGQSPAPAGKPFDIVMLKQYLHIVIKR
                     IWLIALCFVVAEAVMVVMLMRQEDTYSSTATLMFSEGLPIPAQLQTRETRTMGDYIAT
                     QQRIIETKVIQKRAMDRTGYSPADARRLLIRASVAPVGKTSILAITVKSRDPVFSAAY
                     ANALAEEYIDSKAEERIGTSESTVIGLTRQADRLREELRRADEKVLAFKKDNRVIAIR
                     ERGNIAAEYLAKLSSRAAAYRTERMLLEAEQPLISDASDEIVLAALSSPQPSMAMSGT
                     PAIADLAAGTNIAVAVPHGPAGLLERGVVSQPGWQGLRREKARMESELVELRAKFRDA
                     HPVIQQYLARLKEVDRQIDVELKFALSQYYANLEALAIKEEAVRRVEKEWEEEALRVA
                     RKSHEYENLQRNVERLQSLYDLIFNRLKELDISIGIEPESVRIMERAQAASSPDQPRR
                     LKNIFMAALIGLGAGLALVFGIEYIDDSLRYPEEITRDLHLPFFGVVPSAAWDAEDMR
                     SHLLSNIDQKSGLAESYRNIRSSIVSPAAPARVMKTLAITSAVPREGKTTSAVNVALS
                     LAMAGEKILLIDGDMRRGELHKYFGLEAGKGLSDVLQGQAKAEAVIQRTSYSNLDLLA
                     TGDFPEHPAELVLHPEFRKLLEYGQRTYDRVIIDCPPVMAVSEAGILTSQVEGVIFVV
                     WAGKTSRKLTQLAVQVLKQRGANIVGCILNNLEFGRVGYYYYSTYYGYYDYEYLYEHQ
                     HEDKS"
     gene            complement(5395..6600)
                     /locus_tag="PKK36_03240"
     CDS             complement(5395..6600)
                     /locus_tag="PKK36_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNR93606.1"
                     /translation="MKTAGTGRNGLRIHQILAALAVTAVLLAPCGHAQTSAQAEAGDY
                     VSHRILPGDRLSISVDEQPDLNAEYAVAGDGTIDFPMIGRIHVAELTTERASDLIENE
                     LEAKYFKDATIRVNVSEFVEGAILMQGALNQPGSIPFRGDRNITLLEAIAMCEGLSDQ
                     AAGTEVRILRWKPGGGMERQVITVDVQAMTQNMDFSNDQFLRPRDIVMVPTLDATQAR
                     EEFLALGQLGSPGFYPYSENMDVIRAITRAGGLTMHAQLSAARLLRRKADGSYEAITL
                     DLSRLFGAGDMAMNYPILPGDIIFVPTQGQASGGMVYILGAVMNQGAVELPLEQQVTL
                     AKTILKAGGFAKFANENKVKVLRTAPDGSKQTLIVDVGEILKTGLFEEDIPLKAEDVI
                     IVPEKILGF"
     gene            complement(6736..8052)
                     /locus_tag="PKK36_03245"
     CDS             complement(6736..8052)
                     /locus_tag="PKK36_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93607.1"
                     /translation="MLFPASSFAQALRLGPLDIDAKARTEAVYTTNVEQERESEATAD
                     REDVYFVVGLDLIGTALVQPRTELKLETGIAIEKHLNRDDLDNSENPFGSFRAASKTE
                     IGRATVINAEAHYEKTAESEDDSYDLPSRTRATPDGGTETVSINRKKRKVHDAFGYSA
                     DGAWNWKRLSLNGGYQVESERYEEEEDQVDDQDESTLTFGAILELTSILSLTYDYENT
                     KTETPNVEDDWEGWEETQTVGLDLTVYESDLATFTYTLALEKEDQQDEEGDWEPLHGF
                     SLNGDILDRPRFNLSYSMSYDYEEDPEEDDIGFTYDVRASHQITESIKHSLSLTREPY
                     DTLGSTEDTDNTTYAYDLIFSDLFIKNLELGISASYEIDEPMGPDAGDTETERTTIYG
                     LRLAHSRQVTERIEQRLAYTYDLEDSNLEEELLEEHRIVLTYEYTF"
     gene            complement(8194..8685)
                     /locus_tag="PKK36_03250"
     CDS             complement(8194..8685)
                     /locus_tag="PKK36_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93608.1"
                     /translation="MDRKGIGNTPVCQLTARKFSFSFPLNLSSSADYENLSAPNAWKG
                     EDMKKLAVLLTVVAAGVLLAGCATNPSKTINGIELGMTPKKVLDKMGEPTVKRAAKVY
                     ENGQWTQVWEYTSPLLSMNPKTFWLYFENDKLVQWGQPGDFSGKSGANVPVTEYSDQK
                     MIK"
     gene            8903..10477
                     /locus_tag="PKK36_03255"
     CDS             8903..10477
                     /locus_tag="PKK36_03255"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNR93609.1"
                     /translation="MKKDDKNRVIIFDTTLRDGEQCPGASMGLREKMEVGKVLARMNV
                     DVIEAGFPIASPGDFEAVRALAEKIRGPEICGLARCLSKDIECCAEAVAPAGKRGRIH
                     VFLATSAIHRKFKLKKAEKEIIKLAVTGVQHARKFTPNVQFSPEDASRTEPEFLAEVV
                     RAVIDAGATTINIPDTVGYSIPSEFGQVIAGLYDHVPALNKVTVHVHCHNDLGLAVAN
                     SLAAVQNGARGIECTINGLGERAGNCSLEEVVMAIRTRADRMKVWTGINTCELYHASR
                     LVSQLTGMVVQRNKAIVGANAFAHESGIHQDGILKERTTYEIMRPQDVGISGTDLVLG
                     KHSGRHAFRDHLEKMGFHLSADEMERAYGAFIRLADKKKTVYDDDLVSIIQDELSEIP
                     QYYTLEYLHVASGTDTIPTATVRLKKKGEIIQDAACGDGPVDAALKTIDRIAEVKGQL
                     LDYSLQAVTRGKDAVGEVSVRVDFGSRILNGKASSTDIVEASAKAYLNCVNSFIVQEN
                     AGGKKPSKKRNARKKK"
     gene            10478..11332
                     /gene="aroE"
                     /locus_tag="PKK36_03260"
     CDS             10478..11332
                     /gene="aroE"
                     /locus_tag="PKK36_03260"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNR93610.1"
                     /translation="MKISGKTKPYAVLGHPVGHTLSPVMQMAAFRALRMDAVYLALDV
                     APDRLMQVLPAMQAMGFGGANLTVPLKEIAFRGLKTLDASAKRVGAVNTVAFRGDEVM
                     GYNTDGEGLLRALKEAFGSAPEGLTVFVAGTGGAGRAAALMCARNGADSIRLFDTDGG
                     RARRVAEDIQKDQKGFPVSVISESPEAGARECDLIIQATPAGMKPGDAVPLLPEAFRE
                     GQFVYDLIYMYPETPLLAAARRAGAQTASGLGMLLHQGAAAFEIWTGIKPPVDVMRKA
                     LEEAVYHA"
     gene            11325..12173
                     /locus_tag="PKK36_03265"
     CDS             11325..12173
                     /locus_tag="PKK36_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNR93611.1"
                     /translation="MLDAQTIEATPAAMHFITLFVLFFGACIGSYLNVCIYRIPREES
                     TVRPRSHCPGCGQLIAWYDNIPLLSWIVLRAKCRHCGGRISPRYILVEALVAILFLLV
                     WIQYGLSPKTPVYWLMMGGLVLGTFVDLEHMIIPDRVSLGGLAAGLLLSPLIPSLHGK
                     SAWLDALIASAIGAALGGGLLYGVGVLGKMAFKKDAMGLGDVKLLAGLGAFLGWQAVL
                     FIVMVSSFIGAFVGMGFIVAKKHEWQSRIPYGPYIALAAMIWILWGPQVWHWYSALLS
                     GGAPMP"
     gene            12170..12694
                     /locus_tag="PKK36_03270"
     CDS             12170..12694
                     /locus_tag="PKK36_03270"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNR93612.1"
                     /translation="MTKTNIMLVGFMGTGKTTVGRMLAERLNKTFVDMDHKIEERAGK
                     KIADIFAQEGEAHFRALERALVQELAQEENLVVAAGGGVVLNPMNLEDFSKTGHVICL
                     KASHETILRRVLKSSHRPLLEEGDKRQKIIELMKEREPLYDAVPLSVNTACLSAREVT
                     DDICRLLEQQNTSD"
     gene            12802..13104
                     /locus_tag="PKK36_03275"
     CDS             12802..13104
                     /locus_tag="PKK36_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93613.1"
                     /translation="MKKTIIPLVLGLGLILASGCEVGEEDDRDLEFVNASHQRVEVIP
                     FTIEWTGFTLMPGEKKVIRDVTTLDFRLRPDATVQESTASTDRKVIIVDKLKAASR"
     gene            13288..14115
                     /locus_tag="PKK36_03280"
     CDS             13288..14115
                     /locus_tag="PKK36_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93614.1"
                     /translation="MSKLATPFIIIVLILSIVALVLGIQLFNQREILKGRTQRLENAA
                     AEFAKSIHYDDFNAAQLKDYAQMLTPLNQLAAQGANRWDELQDTKLDLENTKAELTST
                     RETLAATEEKLVATEAQVVDLTSRLESKEVELAQANTQIEQLEVEKEDLNAQLADTQS
                     QLDNAKSEATDLKDEIASLNQTIEDMSRELGELPEKVAPRGLTGKILFINPYWNFVVL
                     DIGRDDGLSPNVEMLVHRKDKLIGKVRISAADNKMAIAEILTDWEVMPPKEGDYVLF"
     gene            14102..14236
                     /locus_tag="PKK36_03285"
     CDS             14102..14236
                     /locus_tag="PKK36_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93615.1"
                     /translation="MFSSKILSVLAAVVIVLFLALITLQAVELSHYKSEPSVWPASAL
                     "
     gene            complement(14414..15169)
                     /locus_tag="PKK36_03290"
     CDS             complement(14414..15169)
                     /locus_tag="PKK36_03290"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HNR93616.1"
                     /translation="MRTFRPYFAVIILMALTSIALELSVDVNITDEAGIRVELPDRIA
                     AWEGNELRYCHNKECLKAFLVNDIEDRNVCPACGGPLSGMSYDEAAVLPGDTVLMKKR
                     YVHPTGRQIFVSIVLSGASRSSIHRPQMCLTGQGQVILKSEVLEVPIEGRKPLDVMVL
                     DLEPQGRAEGMTGLTTYYAYWFVGKGRETPSHVQRMIWMSTDRIFHNVAHRWAYIAVS
                     GAREEDSVAYLDEIREFIGGLYPHIALTENIIN"
     gene            complement(15130..16119)
                     /locus_tag="PKK36_03295"
     CDS             complement(15130..16119)
                     /locus_tag="PKK36_03295"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNR93617.1"
                     /translation="MKEIDEKIWSERWNLAALAAEDFIRIGLTSVIIGLIFVMYHFQG
                     NTEDIPSYGRSAFGWLVQRWNSPYSNFSHGWIIVPASLFLVWKKRRDLAAAFPSRSVC
                     RKGLAIVVMALFFHWLGVRVTHPRISVMALIMLLWGIPYYFWGWRVAKELIFPCSYLI
                     FCIPLNFLDTLTFPLRIVATKMSVLLINGLGVAVRRVGSGIYSETAQGFKLDVADPCS
                     GLRSLLAMTALTAIYAWFTQKTILKKWVLFACAVPLAIAGNIVRITALALVAEAFGEE
                     VATGLYHDYSGYIVFAVAILLMVGLGNLIDMDFRKVWNKWKCELSGRTSPSLS"
     gene            complement(16155..16700)
                     /locus_tag="PKK36_03300"
     CDS             complement(16155..16700)
                     /locus_tag="PKK36_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR93618.1"
                     /translation="MKTAAKEQKCVFFDRDGVVNRAPSPEEYYVLSLERFFILPAFME
                     SLRIVNKKGYAAVIVTNQRCIERGLITWDELNRIHDYLRLSVKEAGLELLDILVCPHG
                     ADQCECRKPKPGLFLEASRRYQLDLAASWMVGDSETDVEAGRAAGCRTVRIAHPGAIT
                     SADFHLEDMSLLPDFLSSHLT"
     gene            complement(16684..19047)
                     /locus_tag="PKK36_03305"
     CDS             complement(16684..19047)
                     /locus_tag="PKK36_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93619.1"
                     /translation="MIRKVKPLILLCCLFGISLEAALAPHEVVLLVNENSPRSLEVAN
                     HYVSLRHIPARNVIRLALPDAVRRPEATINESEFLQYIWVPANEEILRRGLGEQVLAW
                     IYSADFPVRVTRAKGAAVSLTGITFVRNRLPADALIVQGKYVSPLFCGPASRKDLRVK
                     LAESLDSLGHRLGAEMPLPAMMLAHTGTRGLSVEEALGVLRRGAASDGTFPRGPVIFV
                     KREDVRSSCRDWQFPAAAEAVKSLRGRAEILDRYPAGQTGLLGVFDGVHEIAPPPGSF
                     LPGAVAEHLTSHAANFSDHNQTKLSVWLRAGATASSGTVVEPYAIGSKFASPFFFAHY
                     LSGCSIIESYYLAVQSPLQLLMTGDPLAAPWAAKFPVVLAAMEDGYLKGKAMFYTDTG
                     LQNPRLPPRIQYILDGVLKNDFHSGPMLSVDTTKLSDGYHELRVSVRKQGPVAEHSFA
                     VRGITVNNRGRAVDLKGLLPGQKVDVHHPVAVTIVGTPSPRETILMAGEMRIPLEGGC
                     FDPAILGAGPVSLQACAIYEDGMEVRSPPASIEIGRFNQPPAIRGVVPTGDGGLRATA
                     EDPEGDSIRYSWFRRMEADRCNARGGLVQDGVLVPETAAPISICIFSDGTESSSEISA
                     EIMVPAGRAAGLLHGAGILFDVQDEKNYGWFGASGKTGGWTLGVCRDGLWKELEAFGA
                     GIPLNTWIELSVRRRPDGVVEGMVDGERVCETAMQKPGWSGQFGLWVEDRPLQFRNVM
                     TCIRDGSAEVSAELMRGAADGSILVRADDGVSASIKTVETAGNEDGS"
     gene            complement(19044..20399)
                     /gene="wbaP"
                     /locus_tag="PKK36_03310"
     CDS             complement(19044..20399)
                     /gene="wbaP"
                     /locus_tag="PKK36_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HNR93620.1"
                     /translation="MGGILLGDALALLAAILLAGLVRYILRESHIPPSRALYLIPLWA
                     VGAIIFKIAPPWGIGPIEELRRIQVLLLVIFGLAAAAMFLTKTGDVTSRIKFALMYLI
                     SVPLVPFTRTLVKRVMLKRRCWGVPTAVYGNDKTVSHVLDVITQEAGLGYIPIGIFDD
                     DLKPSDRVRGIPVLGGLEDSTLEASFALLGAPSIARERLMMLLEGPLSHYRRVIIIPD
                     LFDIPSLWVSARDFLGVLGLEVERNLLNPIARISKQAMELIAVLALAPLWIPVCLLLA
                     LLIRMQTGASPLYRQERVGRHGKLFKTWKFRTMVPNAEEVLEKEMSEHPELRAEWNAH
                     YKLKNDPRITPIGAFLRRSSLDELPQLINVLRGEMSLVGPRPLPKYHQQQLPRSVCAL
                     RESVLPGITGLWQVSGRSETGTEGMERWDTYYVRNWSIWLDTVILVRTFRALLTREGA
                     Y"
     gene            complement(20538..21740)
                     /locus_tag="PKK36_03315"
     CDS             complement(20538..21740)
                     /locus_tag="PKK36_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93621.1"
                     /translation="MTESPGKSAGRILVVVENLPVPFDRRVWMESTTLAQAGYQVCVI
                     CPTGKGYESLREDIDGIHIFRHRLPPEKSSALGYFREYSSALWAEWRLARKVWKEFGF
                     DVIHACNPPDLIFLVALWFKLLHRTKFVFDHHDLTPELYESKFNRRGFFYHMMRLAER
                     LTFALADRVISTNESYREVAIRRGHKRPEHVQVIRSGPDLSRFNPVPPDGRFKKGRKW
                     LVGYMGVMGEFDGVDHLVRAAHHLIAARGREDIQFCFIGGGPCLESLKELAGRLGISP
                     YVEFTGRVPDAEMISRLSSCDVCVDCDPLNPLNDKSTMNKILEYMALGRPIVQYDLLE
                     GRRSAGDASLYARPNDIEDLAEKISDLLADEVKRSVMGKIGRERMINELEWRHQAPKL
                     IQFYSRLV"
     gene            complement(21721..23019)
                     /locus_tag="PKK36_03320"
     CDS             complement(21721..23019)
                     /locus_tag="PKK36_03320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNR93622.1"
                     /translation="MKISVFGMGYVGVVTSACMARDGMQVVGVDVSREKVDMIARGQS
                     PIIELGLSELLDEAVKSGGLTATSDAERAVAQTEVSLVCVGTPSRPDGSLDDSYVMAV
                     CDQLGRAVAAKKQPHTVIIRSTILPGTTARCAERLRAGAKGQTVRVAFNPEFLREGTA
                     IRDYDNPPYTVVGTDDADAEEVLRRVYASVNAPLIVTDVAVAEMIKYTANAWHAAKIT
                     FANEIGRVAKAAGVDARRVMEIIAEDTKLNVSPVYMRPGFAYGGSCLPKDLRAINHFA
                     RTRDLGLPMLGALDVSNRQQISITLDRIAGTGCRKIGLLGLAFKAGTDDLRESPAVEL
                     AERLIGKGYDLKIMDRHVQEARLIGANRSYIEEKIPHLSALLADADEIAAHAELLVIC
                     NTTAEFRNVVASAPKSVRVLDLVGLFKARPEGVNYDGISW"
     gene            complement(23009..>23382)
                     /locus_tag="PKK36_03325"
     CDS             complement(23009..>23382)
                     /locus_tag="PKK36_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNR93623.1"
                     /translation="RHDGYRRSGAVHERTLELGGNRLRVSDTLAGSGRHHVELRFHFH
                     PACAVELTSPLTAVAMRQGRSLRITLPGGLVPKILHAENDGGWYSAGFMKKEPAFTLA
                     AAADVRLPAALETLIEVLNED"
BASE COUNT         5251 a         6952 c         6390 g         4789 t
ORIGIN      
        1 tcatgttttc ctccacgctg ggcgaatatt cgatttttga ggaggcattc aaggggctgt
       61 atctggcgat cggcgcattt ttcggcctga ttctgttcgg gggcatgagt ctgctgggcg
      121 cgccggtgat gatgacctat ggagtgattc gcggactggg gcaaaccatg ccccactcgg
      181 tgatccccca gttcatcggc gctctgatcg gacgctatta cttccagaag cggctgggcc
      241 tgatctggcg acagtacatt ccggtggtca gcgccggttt cgcatgcggc atggggctga
      301 tcacgacggt cggggtggga ataacatttt tgagcaaggc ggcgattccg ctgccgtttt
      361 aggataaaag atggctgagg ttctgataga aattcgcggt gtgaacaaga tcttcgatct
      421 cgggaaggtc aaggtccatg cgttaaagga tgtggatctc gatgtatacc gcggagagta
      481 tctttctatt atggggccct ccggttccgg gaagagcacg ctgttcaaca tgatcggggc
      541 ccttgatcag ccaacctccg gcaccattcg tgtggcggat atcagcctga cagacctgag
      601 cagccgcgaa ctggcctact tccgcgggaa gcatatcggc tacatcttcc agacgttcaa
      661 cctgctcccc gcctacaatg ccctcgaaaa tgtggccatg ccgctggtct tcaacggatg
      721 ggaggaggcc cgggcccatg cccgcgcggc ggaagtgctg gaggaggtgg gcctgggtca
      781 ccgcatcaca cacagaccgg aggagctgtc gggcgggcag cagcagcgag tggccatcgc
      841 gcgcgcgctg gccaacaagc ccgcgatcat tctggcggac gagcccacgg gaaatcttga
      901 tctgcacacc ggagaggaaa tcatcaggct gctcagtcag ctcagccggg aactgggggt
      961 tacggtgatt tccgccacgc atgaccacaa aatgctggcc acatcggacc gcatcctctg
     1021 gatccgggac ggccgcaagg accggctgga gaagcgggaa gacattcgca tccggatcgg
     1081 cacggtgagc tgataaactt cacgcgggcg ccagaaatgc ggcatccacc tcgatggcca
     1141 ttgaataatt aatcatcgtg atattgatga ccagccgcgt tttgccatgc atgtgttcaa
     1201 caactccctc aaaatccttg aactggcccg atttgatgcg gacaagactg cccttcttca
     1261 gatagggaag cacctccacc accttctcgg ccttgagcgc cgtctcgatc tgtttcagtt
     1321 cgcgaaccag ccgctcctgc tcaaagacct gaagcagatt ggccacatac tgcgactgct
     1381 tcagcccggc atcctgctcg ggccgcagga tgcagaacac atagccgggg aaaagaggat
     1441 tttcgaactc gcgcacgcgc gcgccgtagc gatgaagctt cctgatctgc ggcagatgaa
     1501 cggggatgtg ctgctcctcg cagtaacggc aaagtttttt ttcgcagcgc ggacgggtgt
     1561 gcaggacggc ccagcggagc ggtttttcgg gcaggacaag gtcacagacc cattttcttt
     1621 ccggggcggg agcatccgaa tcaggcattg gccgtctccc cggatgccgg gccagccgga
     1681 gatgccgctg tctgcggaac ggatccgttc tccggctcct cggcatatcg gagcccgaca
     1741 atgattgtgc ggaaggtccg cagcagaatc agcacatcca gatacaggct catgtgcttg
     1801 atataataga ggtcgaactg gagcttgcgc tttgtggcct caatgctggt ggcatagggg
     1861 aacttcacct gggcccagcc ggtgatgccg ggcgccacca tcaggcgttc ctgataaaag
     1921 ggaatgcttt gcttcagcag cgcaacaaat tccggccgct cggggcgcgg gcccacaagg
     1981 ctcatttctc cgcgcagcac attgatcaac tggggaattt catcaatgcg ccattttcgc
     2041 agaaaccggc ccaccggcgt tatccggcga tccccggccg tggcccagac cgcacccgtc
     2101 tttgcctcgg catcctgacg catggttctt aatttaagaa gagtataaac acggccctcc
     2161 ctgcccacgc gcttctgccg gaaaaatacc ggtcccggag acgtcagctt gatgagtatc
     2221 gccgcgatca cacccacggg aacaagaaca atcagcccga tcaccgatgc agacagatcc
     2281 atcacccgct tgatctgccg gatatgaatg cgcgaactgt tcatggcggc atgcatgagc
     2341 cattcgtcat caatgtgatc gatgggaatt tccgctccga gttcctcata aagagaaaca
     2401 taatccagta tctgcaggcc cgcataacgc agcggccgca ggacatgaag cagagtgtga
     2461 gccttgtcgg ggggtgtggc caccaccagc gtttcgacat cataggccgc gacgtattcc
     2521 cgcaagctgg cgatgtctcc cagcacggga acgccgcgca cgaaagcctc cgcgtcgccg
     2581 ggctcaaccg aaacgatccc gtacagcgaa tagccggaat tgggctccgc ggcgagcagg
     2641 gccaggactt cttccgcctc gcggccgccg ccaagaatca gggtcttttt cgaaaacaac
     2701 ccgtagccaa gcgccatgcg cagaagacgg cgggcaaccc atgagccgac cgcgataaac
     2761 agcgaggcca ggagcaggac gccacggccg atatgcagct cgaaatcggc atagaacgcc
     2821 agaattgtga ccacaagccc cagcgccgtg acgaccaccg gcaggaaaaa agaggcaccc
     2881 agcttctggg tgatcacctc ccgctcatac atgccgccgg catagaatat gagaagaaag
     2941 acaaaagaca cagaagtcag cggaagcaga ttgtccccga gatattccag gccgatcttc
     3001 catcccaggc gcataatcgc agacaggaac acggccagga caatggtcag cacatcggat
     3061 acaaaaagcg ccatccgccg cactgaaaac cgggatgtcc gtccaaaata cattcctctt
     3121 tgtatccttt actcaaacga gcgggcccgt ctgctcagct tttatcctca tgctgatgct
     3181 cgtaaagata ttcgtaatca taatatccgt agtatgtcga atagtaataa taacccaccc
     3241 ggccgaattc cagattgttc aagatgcagc cgacgatgtt tgcgccacgc tgcttgagca
     3301 cctgaaccgc cagctgagtg agttttcttg atgttttacc cgcccatacc acaaagatca
     3361 caccctccac ctgcgaagtc aggatgccgg cttcagacac cgccatgacg ggcgggcagt
     3421 ctataatgac acggtcatat gtccgctgac cgtattccag cagtttcctg aattctggat
     3481 gaagcaccag ttccgccgga tgctcgggga agtcgccggt tgccagcaga tccagattgg
     3541 aataggaggt cctttgaata accgcctccg ccttggcctg gccctgaaga acatccgaaa
     3601 ggcccttgcc ggcctcgagt ccgaaatact tgtgaagctc gccgcgccgc atgtccccgt
     3661 caatcaggag tatcttctca cccgccatgg ccagactcag cgcaacgttc accgcactgg
     3721 tggttttccc ttcgcgcgga acggcggagg taatggccag cgttttcatg acccgcgcgg
     3781 gggccgcagg agaaacaatg gaggagcgga tgttgcggta ggattccgca agcccgctct
     3841 tctggtcaat attgctcagg agatgcgaac gcatgtcttc cgcatcccat gcggcggatg
     3901 gcacaacccc gaagaaggga agatgcaaat cacgggtgat ttcctcggga tagcggaggg
     3961 agtcatcgat atattcgatg ccgaacacca gcgcaaggcc tgcgcccagt ccgatcaggg
     4021 ccgccatgaa tatgtttttc aggcggcggg gctgatccgg cgaggatgcc gcctgggcgc
     4081 gctccatgat ccgaacggat tcgggttcga ttccgatgga tatatccagc tctttcaggc
     4141 ggttgaaaat gaggtcatac agactctgaa gccgttccac gttgcgctgc agattttcat
     4201 attcatgact ctttcgggca acccgcagcg cctcttcctc ccattccttc tccactcggc
     4261 gaacggcctc ctccttgatg gccagcgcct caagattggc ataatactga gacagggcga
     4321 atttgagttc cacatcaatc tggcggtcca cctccttgag ccgtgcgaga tactgctgga
     4381 tcacgggatg ggcgtcacgg aacttcgccc tcaattcaac cagttcagac tccatgcgcg
     4441 ctttttcgcg gcgcagcccc tgccagcccg gctgactgac cacgccccgc tcgagcagtc
     4501 ccgccggccc gtgcggcaca gcaaccgcaa tattggtgcc ggcagccaga tcggcaatgg
     4561 ccggcgtccc gctcattgcc atggaaggct gggggcttga aagtgcagcc agaacaattt
     4621 catcagaggc atcgctgatc agcggctgct cggcctccag cagcatgcgc tccgtccggt
     4681 atgcggccgc ccgggatgac agcttggcga gatactcggc cgcgatgttt ccgcgctcgc
     4741 ggatggcgat cacacgattg tctttcttga acgccagcac cttctcgtcc gcgcggcgca
     4801 attcctcgcg caggcgatcc gcctgccggg tcagcccgat caccgtggat tcggaagttc
     4861 ctatacgctc ctccgccttg gaatcaatat actcttccgc aagagcgttc gcataggcgg
     4921 ccgagaaaac cgggtcccgc gatttcaccg tgatggccag aatggaggtt ttgccgacag
     4981 gagccaccga cgcacgaatc agcagtcttc gggcatccgc cggagaatac ccagttctgt
     5041 ccatggcccg cttctgaata accttggttt cgatgatgcg ctgctgcgtg gcgatataat
     5101 cacccatggt gcgggtttcc cgcgtctgca gctgcgccgg aatcgggagg ccctcgctga
     5161 acatcaaagt tgccgtggaa gagtaggtgt cctcctgacg catcagcatc accaccataa
     5221 cggcctctgc caccacaaaa cagagcgcaa tcagccatat gcgcttgatg acaatatgca
     5281 gatactgctt gagcatcaca atatcgaacg gcttgccggc gggagcggga ctctgcccgg
     5341 ggacctgtcc ggcggcatgg ggagggctag gaggctcgat gatacctttc ataatcagaa
     5401 tcccagtatt ttctccggca caataatcac gtcttccgcc ttgaggggaa tgtcctcctc
     5461 aaaaaggccc gtctttaaaa tttcgcccac atccacaatc agcgtttgtt tagatccgtc
     5521 cggggctgtc cgaagcacct tcactttgtt ttcatttgca aattttgcaa agccgccggc
     5581 tttcagtatg gttttggcga gcgtcacctg ctgctccagc ggcagctcca ctgcgccctg
     5641 attcatcacc gcacccagaa tatacaccat tccgccgctg gcctggccct gggtgggcac
     5701 aaagataata tctccgggga gaatgggata attcatggcc atgtcgccgg caccgaacag
     5761 gcgggagaga tccagggtaa tcgcctcata agatccgtca gccttcctgc gcaggaggcg
     5821 cgcggcgctt aactgtgcgt gcatcgtcag acctccagca cgggttatag ctctaatcac
     5881 atccatattc tcagaatagg gataaaagcc gggcgaaccc agctgaccca gcgcaagaaa
     5941 ttcctccctc gcctgtgtgg cgtccagcgt gggcaccatc acaatgtcac gcggccggag
     6001 aaactggtca ttgctgaaat ccatgttctg cgtcatggcc tgcacatcca ccgttatcac
     6061 ctgccgctcc atgccgccgc cgggcttcca ccggagtatt cgcacttcgg tacccgcagc
     6121 ctggtccgaa agtccttcgc acatggcaat cgcctccagc agcgtgatgt tgcggtcacc
     6181 ccggaaagga atgctgcccg gctgattcag ggcgccctgc atcaaaatgg ccccctcaac
     6241 gaattcagac acgttcacgc gaatcgtggc atccttgaaa tattttgctt caagctcgtt
     6301 ttctatcagg tcggaggctc gctccgttgt cagttccgcc acgtggattc ttccaatcat
     6361 tgggaaatcg atggtgccgt ctccggccac ggcgtattcc gcattcaggt cgggctgttc
     6421 atcgaccgaa atgctgagcc ggtcgccggg aagaatgcgg tgggaaacgt aatctccggc
     6481 ttccgcctgc gccgaagtct gggcatgtcc gcaaggggca agcaggaccg ccgtcaccgc
     6541 cagcgcggca agaatctgat gaatccgcaa cccgttccgc cccgtacccg ccgttttcat
     6601 attcgtccac tccataatct ttacagctca cctcagcagt atacagccga acctcggaaa
     6661 tatatccaga acattttgaa aaaatacgga ctaaacggcg cgacaagcgc ccgttcccgt
     6721 ataattccag cggggtcaga acgtgtactc gtaagtcagc acaattcgat gttcttcaag
     6781 aagctcctct tcaagattgg agtcctcaag atcataagtg tacgccagcc gctgctctat
     6841 tctttctgtg acttgacggg aatgcgcaag cctcaaccca tagatggtgg ttctttccgt
     6901 ctccgtgtcg ccggcatccg gccccatggg ctcgtctatt tcataggatg cgctgatacc
     6961 cagttccaga ttcttaataa aaagatcact gaagatcagg tcatacgcgt acgtcgtatt
     7021 gtccgtgtcc tcggttgagc ccagcgtgtc gtagggctcc cgtgtcagag aaagcgaatg
     7081 ttttatgctt tccgttatct ggtgagacgc ccggacatca taggtaaaac cgatatcatc
     7141 ctcctcggga tcctcttcat agtcatagct catggaataa ctcaaattga aacggggcct
     7201 gtccaggatg tccccgttca ggctgaagcc atgaagaggc tcccagtctc cctcctcatc
     7261 ctgctggtct tccttttcaa gagccagggt ataggtaaac gtggccagat ccgattcata
     7321 gaccgtgagg tccagaccca cggtctgcgt ttcttcccag ccctcccagt catcttccac
     7381 gttgggcgtt tcggttttcg tgttttcgta atcataggtg agcgacaaga tgctggtcag
     7441 ctccagaatc gcgccaaagg tcagagtgga ctcgtcctga tcatccacct ggtcctcctc
     7501 ttcttcatag cgctcggatt caacctgata accgccattg agagacagtc tcttccagtt
     7561 ccacgccccg tcagccgaat agccaaaggc atcatgcacc ttgcgcttct tgcggttgat
     7621 ggaaaccgtc tctgtgccgc catccggagt ggctctcgtg cggctgggga gatcatagga
     7681 atcgtcctct gattcggccg tcttttcata gtgggcttcc gcattaatca ccgttgcccg
     7741 gcctatttcg gtttttgacg ccgcccgaaa cgaaccgaac gggttttccg aattgtccag
     7801 atcgtcgcga ttgagatgct tctcgatggc gatgccggtt tcgagcttca gttccgtgcg
     7861 gggctgaacc agtgccgttc caatgagatc aagcccaaca acaaaataga catcttcccg
     7921 gtcggctgtc gcttcggact cccgctcctg ctcaacgttt gtggtgtaaa cagcctcggt
     7981 ccttgccttg gcatcaatat ccaggggccc cagacggaga gcctgggcaa acgaagacgc
     8041 cggaaacagc aacgcaacag ccagccaccc aaacccgccc ccccatatgc ggcgggagaa
     8101 tgacagcctt tttttcagac gggtgttatg caatataaag tcctccgcaa agtttgcgca
     8161 tgcgcggcat agggccgggg gggctcacgt cggctatttg atcatcttct gatcggaata
     8221 ttcagtgacc ggcacgttcg ctccgctctt gccggaaaaa tcacccggct gaccccactg
     8281 caccagtttg tcattttcaa aataaagcca gaaggtcttt ggattcatgg agagcaaagg
     8341 agaggtatat tcccaaacct gcgtccactg gccgttctca taaacctttg cggcgcgctt
     8401 caccgtgggc tcacccatct tatccagaac ctttttggga gtcatgccca gctcgattcc
     8461 gttaatagtc tttgagggat ttgtggcgca gccggccaga agcacacctg cagcaacaac
     8521 agttaaaagg actgccaact tcttcatatc ttctcctttc catgcgtttg gagccgacag
     8581 attctcataa tcggccgaag aagacaagtt caaaggaaag ctgaatgaga attttctcgc
     8641 agtcaattga caaaccgggg tgtttccgat accttttctg tccatgaaat acaagttcgc
     8701 catgtcgaat caggatcagg gagccagact gctgctggcg ctgctgctcg cccttctcga
     8761 ggcttgcgac gcctcgggac ggggtatttc tatctagccc gtccctaagc ccgcaagccg
     8821 tctcaaatta ttggtgcatt aaacacgtga tttttataga ttgttgggcg gctgtatcgc
     8881 cttgaaggac tggaagcgga ttatgaaaaa agacgacaag aacagggtta tcatatttga
     8941 caccacactg agggacgggg aacagtgccc cggggccagc atgggtttgc gcgagaaaat
     9001 ggaagtcggc aaggtgctgg ccagaatgaa tgtagatgtc atcgaagctg gatttcccat
     9061 tgcctcgccc ggtgattttg aggccgtcag ggcgctggct gaaaaaatca ggggccccga
     9121 aatatgcgga ctggcgcgat gcctgagcaa ggacattgaa tgctgcgcgg aggcggtggc
     9181 ccccgccgga aagcggggaa gaatacatgt ctttctggcc acttccgcga ttcaccggaa
     9241 gttcaagctg aaaaaggccg aaaaggaaat catcaaactg gccgtcacgg gcgtgcagca
     9301 tgccagaaaa ttcaccccga atgtccagtt cagccccgaa gatgcatccc ggacagaacc
     9361 tgagtttctg gccgaggtgg tgagagccgt gattgatgcc ggagccacca cgatcaatat
     9421 tccggacacg gtggggtatt cgattccgag cgagtttggt caggtaatcg ccggtttgta
     9481 cgatcatgtg ccggcattga acaaagtgac cgtgcatgtc cactgtcaca atgacctggg
     9541 cctggcggtt gccaactccc tggctgccgt gcagaacggc gcacgcggca ttgagtgcac
     9601 cataaacggc ctgggggaac gcgccgggaa ctgttcactg gaagaagtgg tcatggccat
     9661 ccgaacacgc gccgaccgga tgaaggtctg gacgggcatc aacacatgtg aactgtatca
     9721 cgccagccgg ctggtcagcc agttgaccgg catggtggtt cagcgcaaca aggccatcgt
     9781 tggcgccaat gcctttgcgc atgaatcagg aatccatcag gacggaattt tgaaggaacg
     9841 caccacctac gaaatcatgc ggccgcagga tgttggcatt tccggaacag acctggtgct
     9901 gggcaagcac tccggccgac atgccttccg cgatcacctg gagaagatgg ggttccacct
     9961 gagtgcagat gagatggaaa gagcatacgg ggcatttatc cgcctggccg acaagaagaa
    10021 gaccgtgtat gacgacgatc tggtttcgat tatccaggat gaactgtctg aaattccgca
    10081 gtactacacg ctggagtatc tgcatgtggc atccggcacc gatacgatcc cgaccgccac
    10141 ggtccgcctg aaaaagaagg gagagatcat acaggacgca gcctgcggag acggtcctgt
    10201 tgatgcggcg ctgaaaacca tagatcgcat tgccgaggtg aaaggacaac tgctggatta
    10261 ttcgctgcag gccgtaacgc ggggaaaaga cgcggttgga gaggtcagcg tccgcgtgga
    10321 tttcgggagc agaatcttga acggaaaagc ttccagtacc gacattgtgg aggccagcgc
    10381 caaggcatac ctcaactgcg tcaacagctt catcgttcag gaaaatgccg gcgggaaaaa
    10441 accatcaaag aagcggaatg cccgaaaaaa gaagtgaatg aaaattagcg gaaaaaccaa
    10501 gccttatgcg gtgctggggc atcccgtcgg tcacaccctg tcgcccgtga tgcagatggc
    10561 ggcgttccgg gctctgcgga tggatgctgt ctatctggcg ctggatgtgg cgccggaccg
    10621 cctgatgcag gtgctgcccg ccatgcaggc catggggttt ggcggcgcca acctgaccgt
    10681 tcctctcaag gaaatcgcct tccgcgggct caaaactctg gatgcttcgg caaagcgcgt
    10741 cggggcagtc aacacggtcg ccttccgagg cgatgaagtt atgggctaca acaccgatgg
    10801 cgagggactc ctgcgcgcct taaaggaggc gtttggctcc gctccggagg gtctgactgt
    10861 atttgttgcc ggcacgggcg gagccggtcg cgcagcggcc ctgatgtgcg cacgaaacgg
    10921 ggcggactcc atcaggcttt ttgacaccga tggcggccga gcccgacgcg tggcggagga
    10981 tatccagaag gatcaaaagg ggttccccgt gtctgtgatc agcgaaagcc cggaggccgg
    11041 ggccagagaa tgcgatttga tcatccaggc cacccctgca ggcatgaagc ccggagatgc
    11101 tgtgccgctg ttgccggaag cattccgaga aggccagttt gtatatgacc tgatttacat
    11161 gtatccggaa acacccctgc tggccgccgc ccgcagggcc ggcgcgcaaa ccgccagcgg
    11221 acttggcatg cttcttcacc agggtgcggc cgcgtttgaa atctggacag gaataaaacc
    11281 gcccgtggat gtcatgcgca aggcgctgga ggaggccgtt tatcatgctt gatgcccaga
    11341 cgattgaagc cactccggcc gccatgcatt tcatcacctt gtttgttctt ttcttcggcg
    11401 catgcatcgg cagctacctc aatgtctgca tctaccgcat tcctcgcgaa gaatccactg
    11461 tcaggccccg ctcccactgc cccggctgcg gacagctgat tgcctggtac gacaacatcc
    11521 ccctgctcag ctggattgtt ctccgcgcca aatgccgcca ctgcgggggg cggatttctc
    11581 cccggtatat tctcgttgaa gccctggttg cgatcctttt tctcctggta tggattcaat
    11641 acggcctgag cccgaagact cccgtttact ggctcatgat ggggggactt gtactcggga
    11701 cgtttgttga tcttgaacat atgattatac ccgaccgcgt ttcactcgga gggctcgcgg
    11761 cggggctgct cctgagcccc ctgatcccgt cgctgcacgg gaaatccgca tggctcgatg
    11821 ctcttattgc ttcagccatc ggcgcggcac tcggcggagg tcttctttac ggcgtgggcg
    11881 tgctcggcaa gatggcattc aagaaggatg ccatgggcct cggcgacgtg aaacttctgg
    11941 ccgggctggg agcctttctg gggtggcaag ccgtgctgtt tatcgtcatg gtctcttcgt
    12001 tcatcggagc ctttgtcggg atgggattca tcgtcgccaa aaaacacgaa tggcagagcc
    12061 gcattcccta tggcccctac atcgcccttg cggccatgat ctggattctc tgggggccgc
    12121 aggtgtggca ctggtattcc gccctgcttt ccggcggagc cccaatgcca tgacaaaaac
    12181 caacatcatg ctggtgggtt tcatgggaac cgggaaaacc actgtcggaa ggatgcttgc
    12241 tgaacgcctc aataaaacct ttgtggacat ggatcataaa attgaggagc gggctggaaa
    12301 gaaaattgcc gacatatttg cgcaggaagg ggaggcgcat tttcgcgccc tggagcgtgc
    12361 attggtacag gaactggcac aagaggaaaa cctggtggtc gcagcgggcg gaggagtggt
    12421 tctcaatccg atgaaccttg aggacttttc aaaaaccggc cacgtcattt gccttaaagc
    12481 cagtcatgaa accatcctcc ggcgcgtgct gaaatcctca caccggccgc tgctggaaga
    12541 gggcgacaag aggcagaaaa tcattgaact gatgaaggag cgtgagcccc tgtacgatgc
    12601 cgttcccctc agcgtgaaca cagcctgcct gtctgcgcgc gaggttaccg acgatatctg
    12661 ccgccttctg gaacaacaaa acacatccga ttgaatctgt gcgggagccc aaattggagc
    12721 aatctgatac ttgacaccgc aaagccgctt tggcaacctg ttcaagtaga tatgcattaa
    12781 taccgtaaga aggatgctct tatgaaaaag acgattatcc ccctggttct tggtctgggc
    12841 ctgatcctcg cgagcggatg cgaagtcgga gaagaagatg atcgagacct cgagtttgtt
    12901 aacgcatctc atcagcgggt ggaggttatt ccttttacga tagagtggac cggctttacc
    12961 ttgatgcccg gtgaaaagaa ggtcatccgg gatgtgacca ctttggattt caggctgaga
    13021 cccgatgcaa cggtgcagga gagcaccgcc tccacggacc ggaaggtcat tattgttgac
    13081 aagctcaagg ctgcaagccg atagctgcca gcccttttct ccccccggcc cttcaggctc
    13141 cccggcccgg catccgggag agggagaacc tgtttcacag acgattcagg gcgaacacca
    13201 attgtgattt gacaaaccac ccggcagccc gctagtatcc ggcagaaaca tacttgcgtt
    13261 ttaggatcta ttgcagagga tgaacgtatg agcaaactgg caacgccgtt tataataatt
    13321 gtgcttattc tcagtatcgt ggcacttgta cttggaatac agctcttcaa tcagcgggaa
    13381 attctcaaag gacggactca gcgccttgag aacgccgccg ctgaattcgc caaaagcatt
    13441 cactatgacg acttcaacgc agcccagctg aaagactatg cgcagatgct cacccccctg
    13501 aaccagcttg ccgcgcaggg cgccaatcgc tgggacgaac tgcaggacac caagctggat
    13561 cttgaaaaca caaaagccga actcacttca accagagaaa ccctggccgc caccgaagaa
    13621 aaactcgttg ccaccgaggc acaggttgtg gatctgacct cccggcttga aagcaaggaa
    13681 gtcgagctgg cgcaggccaa cacgcagatt gaacagcttg aagtggaaaa agaagatctt
    13741 aacgcccagc ttgccgacac acagtctcaa ctcgacaatg caaaaagcga agcgacagac
    13801 ctgaaggacg agatcgcatc cctgaatcaa accattgagg atatgtcccg cgaactcggc
    13861 gaactgccgg aaaaagtggc gcccaggggt ttaaccggaa agattctctt catcaacccc
    13921 tactggaact tcgtggtgct ggacatcggc cgcgatgacg ggttgagtcc caacgttgaa
    13981 atgctcgtgc accgcaaaga caaattgatc gggaaagtgc gcatcagcgc cgccgacaac
    14041 aaaatggcca tcgccgaaat tcttacagac tgggaggtca tgcctccaaa ggagggcgat
    14101 tatgttctct tctaagattc tgagcgttct ggccgcggtg gtgatcgtgc tttttctggc
    14161 cctgataacc cttcaggccg tggaattgag ccactacaag agcgagccgt ccgtatggcc
    14221 ggcctctgca ctataagagg gaaatccggt gaaggccgtc gtcatttttg cttccgccct
    14281 catgctgggc gcgttgctgg caggatgcgc aacacccgac ggtgaaacgg aacttccgtg
    14341 gaatacgcct cagtcatggg aggcatcgcc tatgattccc ggcatggacc gttattgaca
    14401 gagttctcaa atatcagttg atgatgtttt ccgtcaaggc aatgtgcggg tagagccccc
    14461 cgataaactc ccgaatttca tccagatagg cgaccgaatc ctcttcacgc gcgcccgata
    14521 cggcaatata ggcccagcga tgagccacgt tgtggaatat gcggtcagtg gacatccaaa
    14581 tcatgcgctg cacatgcgac ggagtttcgc gccctttccc gacaaaccag taggcgtaat
    14641 aggtggttaa tcccgtcatg ccctccgccc gtccctgcgg ctcaaggtcc agcaccatca
    14701 catcaagggg cttccgaccc tcaatgggaa cttccagaac ttcacttttc aggatgacct
    14761 gcccctgccc cgtcaggcac atttgcgggc ggtgaatgct tgaacgggag gctccgctca
    14821 gcacaatgga aacaaaaatc tgccggccgg tcggatgcac ataacgcttt ttcatcagca
    14881 ccgtgtcgcc cgggagcacc gccgcctcgt cataggacat gccggaaagc gggccgccgc
    14941 atgcggggca cacgttccgg tcttcaatgt cattcaccag aaaagctttc aggcactcct
    15001 tgttgtgaca gtaccgcagt tcattccctt cccaggccgc aatgcggtcg ggaagctcca
    15061 ccctgatgcc ggcttcgtcc gtaatgttca catccacgct gagctccagc gcgatgctgg
    15121 tcagggccat caggataatg acggcgaagt acggcctgaa agttcgcatt tccatttgtt
    15181 ccacactttt ctgaaatcca tgtcaataag gttccccagc cccaccatca gcagaatggc
    15241 cactgcaaaa acaatgtatc ccgaataatc atgatacaaa cccgtggcca cctcttcccc
    15301 aaacgcctcc gccacaaggg ccagggccgt gattcgcaca atgttgcccg caatggcgag
    15361 cggaacggcg caggcgaaga gcacccactt ttttagaatc gttttctgcg tgaaccacgc
    15421 atagatggcg gtcagtgccg tcatggccag cagggagcgg agcccgctgc agggatcggc
    15481 cacatccagc ttgaatccct gcgcggtttc ggaatagatg cccgagccca cgcgccggac
    15541 agccaccccc agaccgttaa tcagcagcac agacatcttc gtcgccacga ttcgcagcgg
    15601 aaacgtcagc gtgtctaaaa agttcagcgg aatgcagaag atcagatatg aacacgggaa
    15661 aatcagctcc ttcgcaactc gccagcccca gaaatagtat ggaattcccc aaagaagcat
    15721 gatcagggcc atcacggaaa tccggggatg ggtcacacgc acgcccagcc agtgaaaaaa
    15781 cagcgccatg accacaattg caagcccttt ccggcagaca ctgcgggagg ggaacgctgc
    15841 cgcaaggtcc cgtcgcttct tccacacaag gaaaaggctt gccggaacaa tgatccatcc
    15901 gtgggaaaaa ttgctgtagg ggctgttcca gcgctgaaca agccatccaa acgcggagcg
    15961 cccgtaggaa ggaatatcct ctgtgttgcc ctgaaaatga tacataacga agatcaggcc
    16021 gattatgacc gaagtcagac caatccggat gaagtcctcc gctgcaagag cggcaagatt
    16081 ccagcgctcg ctccagattt tttcgtcaat ttctttcatg gcaaagtcgt gaaactatac
    16141 gccgccccac aaggtcaagt caaatggctg gagagaaaat ccggaagcag gctcatatcc
    16201 tccagatgga agtccgccga tgttattgca cccggatggg caatgcgcac tgtccggcag
    16261 ccggccgcgc ggccggcctc cacatcggtt tcggaatcac cgaccatcca ggaggccgcc
    16321 agatcaagtt gatacctgcg ggacgcctca aggaaaagac ccggtttggg ctttcggcac
    16381 tcacattgat ccgcgccgtg agggcatacc agaatatcca gaagttcaag ccccgcctct
    16441 ttgacggaaa gcctcaaata atcatgaatc cggttcagtt catcccatgt aatcaggcct
    16501 cgttcaatac agcgctggtt ggtcactatc acggcggcat agcccttttt gttcactatc
    16561 cgaagtgatt ccatgaaggc cggaagaatg aagaatctct ccagcgacaa gacatagtac
    16621 tcctccggag acggcgcacg atttactacg ccgtcccggt caaaaaaaac gcatttttgc
    16681 tctttagctg ccgtcttcat tgcccgccgt ttctactgtt tttatcgatg cggaaacgcc
    16741 gtcatctgcc cgcacaagaa tggagccatc tgccgcaccg cgcatcagtt ccgccgaaac
    16801 ctccgcactc ccgtcccgga tacaggtcat aacattccga aactgaagcg gcctatcttc
    16861 cacccagagt ccgaactgtc cgctccatcc cggtttctgc atggccgttt cacaaacgcg
    16921 ttcgccgtca accattccct ccacaactcc gtcggggcgg cggcggacag acaattcaat
    16981 ccatgtattc aacggtatgc ccgcgccaaa tgcctccagt tccttccaca gcccatccct
    17041 gcaaacacca agcgtccatc ctccggtctt tcccgacgca ccaaaccagc cgtaattctt
    17101 ctcgtcctga acatcaaaca ggattcctgc gccatgcagc aggccggcgg cacggcccgc
    17161 gggcaccatg atctcagcgg atatttcgga tgaagactct gtgccgtcgc tgaaaatgca
    17221 tatactgatc ggagcggctg tttccggaac cagaacgccg tcctgcacca gccccccccg
    17281 cgcattgcag cgatctgcct ccatcctgcg gaaccagctg tatcgaatcg aatcgccttc
    17341 aggatcttcg gcggtggcgc gcaatccccc atcgccggtc ggaaccacac cccgaatcgc
    17401 cggcggctga ttgaagcggc caatctcgat gcttgccggc ggcgagcgga cctccattcc
    17461 gtcctcgtag atcgcgcagg cctgaaggct gactggcccg gcccccagaa tggccggatc
    17521 gaaacacccc ccttcaaggg gaatgcgcat ctctccagcc atcaggatgg tttcccgcgg
    17581 gcttggggtt ccgacgattg tcacggcaac cggatgatgc acatccactt tctgtccggg
    17641 gagcagcccc ttaagatcaa ctgcgcgccc cctgttgttg acggtgattc ccctgaccgc
    17701 aaatgaatgc tccgccaccg gaccctgctt gcggacagat accctcaatt catgataccc
    17761 gtccgatagc tttgtggtat ccacggacag catggggcca gagtgaaaat cgtttttcag
    17821 cacgccatcc aggatgtact ggatgcgcgg cggaaggcgg ggattctgca gaccggtatc
    17881 tgtatagaac atggccttcc ctttgagata cccgtcttcc attgccgcca gaaccacggg
    17941 aaatttagcc gcccagggtg ccgccagcgg atcgcctgtc atcagaagct gcaacgggct
    18001 ctgcacggcc agataatagc tttcgatgat gctgcagccg ctcaagtagt gggcaaagaa
    18061 aaagggactg gcaaatttcg agccgatggc atagggctcc accactgttc cggacgaggc
    18121 ggttgcaccg gcgcgaagcc agaccgaaag cttggtctga ttgtggtccg aaaagttcgc
    18181 ggcatggctg gtcaggtgtt cggccactgc gccggggagg aaagagccgg gggggggcgc
    18241 tatttcatgc accccgtcga aaacacccag cagccccgtc tggccggcag gatagcggtc
    18301 aagaatttcc gcgcgtcccc tgagcgattt aacggcttcc gccgctgcag gaaactgcca
    18361 gtcccggcat gaagaccgga catcctcccg tttgacaaat ataacgggcc cccgaggaaa
    18421 cgttccgtct gaagcggccc cgcggcgcag gactccgagc gcctcttcaa cgctcaaacc
    18481 gcgggtgccg gtatgcgcca gcatcattgc gggaagcggc atttctgcgc ccaaacgatg
    18541 acccaggctg tccaatgact ccgccagctt cacgcgcaga tcttttcttg atgccggacc
    18601 gcagaagagc ggagaaacat atttcccctg tacaatcagc gcatccgcgg gcagcctgtt
    18661 gcgcacaaaa gtaattcccg tcagagaaac agccgcgcct ttggcccgtg taacgcgaac
    18721 gggaaaatcg gccgaataga tccatgcaag aacctgttcg cccagccccc gacgcagtat
    18781 ttcctcattt gccgggaccc agatatactg cagaaattcc gactcattaa ttgtcgcttc
    18841 gggcctccgc acagcgtccg gcagggccag ccgaataacg tttcgcgcag gaatgtgccg
    18901 cagcgacaca taatggtttg ccacttcaag cgaccgcggc gaattctcat tgaccagaag
    18961 caccacttca tgcggggcaa gtgccgcctc aagtgaaata ccaaacaaac agcagagcag
    19021 gatcagcggt ttgacttttc ggatcaatac gccccttccc gggtaagaag ggcccggaag
    19081 gtccggacaa ggatgaccgt atccagccag atggaccagt tccggacata atacgtgtcc
    19141 cagcgctcca tgccctcggt gcccgtctcg ctccgcccgg agacctgcca cagccccgtg
    19201 atgccgggca gcacgctttc acggagcgca cagacactcc tgggcagctg ctgctgatgg
    19261 tatttcggaa gcggtcgggg cccgaccagc gacatttctc cccgcagcac attaatcaac
    19321 tgcggcagct catccagaga actgcgccgg agaaatgcgc cgatcggagt gatgcgcgga
    19381 tcgtttttaa gtttgtaatg agcgttccat tccgcgcgga gctcgggatg ttcagacatt
    19441 tccttttcaa gaacctcttc cgcgttgggc accatggtcc ggaatttcca ggtcttgaaa
    19501 agctttccgt gccgtccaac ccgctcctga cggtacagcg ggcttgcgcc ggtctgcatt
    19561 cggatcagca gcgccagcaa gaggcacacc ggaatccaca agggcgcgag cgccagcacc
    19621 gcaatgagct ccatagcctg ttttgaaatg cgggctatgg gattcagcag attccgctct
    19681 acctcaagcc ccagcacccc cagaaaatcc cgggcactga cccacaggga gggaatatcg
    19741 aaaaggtcag gaatgatgat tacccggcgg taatgcgaaa gaggtccctc cagcagcatc
    19801 atcaggcgct ccctcgctat actgggtgcg cccagcaaag caaagcttgc ctcaagcgtg
    19861 gagtcttcca ggcccccgag gaccgggatt ccgcgaacac ggtccgaggg cttcaagtca
    19921 tcgtcaaaaa tgccaatggg aatatagccc aggccggcct cctgcgtaat gacatccagc
    19981 acatgggaga cggtcttgtc attgccgtag accgctgtag gcacacccca gcaccggcgt
    20041 ttcagcatga ctcttttgac cagtgtgcgc gtgaagggaa ccagcggaac actgatgaga
    20101 tacatcagcg cgaatttaat gcggctggtg acatctccgg tcttggtcag aaacatggcg
    20161 gcggcggcca ggccgaaaat gaccagcaac aggacctgta tgcgccggag ttcttcaatc
    20221 ggaccaatcc cccatggggg ggcaatcttg aaaatgatgg cgcccaccgc ccacagcgga
    20281 atcagataca gcgcccggga cggcggaata tggctctcgc gcaggatata gcgcaccagg
    20341 ccggccagaa gaatggcggc cagcagggcc agcgcatccc ccagaagaat cccgcccatg
    20401 ttcaagaaca tccggcggta gtatgaaaat ctatcctgtg aagaatcggc ggtcaattcc
    20461 atgatcccca gcataaacaa ctgccccccg gcgcgcaagc ttgcgttcgc gcgccggggg
    20521 gcagcattcg gggccggcta gaccagtcga ctgtaaaact ggatcagctt cggcgcctga
    20581 tgcctccatt cgagctcgtt gatcattcgt tcccggccga ttttccccat aacggaccgt
    20641 tttacctcgt ccgccagaag atcggatatc ttttcggcca gatcctcgat gtcattgggg
    20701 cgggcgtaaa gcgatgcatc acccgcggaa cgccgcccct caagcaaatc atattgaaca
    20761 atcggacggc ccagagccat gtactccaga attttgttca ttgtagactt gtcattgagg
    20821 gggttcagcg gatcgcagtc cacgcagaca tcgcagcttg aaagcctcga aatcatttct
    20881 gcgtccggca cacggcccgt gaattccaca taaggagata tccccagcct gcccgccagt
    20941 tctttcaggg actcaaggca cggaccgccg ccgataaagc agaactgaat gtcttcccgg
    21001 ccccgggcgg cgatcagatg atgagcggca cgcacaaggt gatccacccc gtcaaactcc
    21061 cccatcaccc ccatatagcc cacaagccat ttgcggccct tcttgaatct tccatcgggg
    21121 ggaacagggt tgaagcgcga gagatccggg ccgcttctga tcacctgcac atgttccgga
    21181 cgcttgtgac cccgcctgat cgccacctcc cggtaggatt cattggtcga gatcacccgg
    21241 tcagcaagcg caaaggtcag gcgctcggcg agacgcatca tatgatagaa aaagccgcgc
    21301 cggttgaact tggactcgta cagctccgga gtcaggtcat ggtgatcgaa gacgaatttt
    21361 gtccggtgca ggagcttgaa ccacagcgcc accagaaaga tcagatccgg cggattgcag
    21421 gcatggatga catcaaaacc aaattctttc caaactttcc gcgccagccg ccactcggcc
    21481 cagagggcgc tgctgtattc gcgaaaatat cccagggcgg aactcttttc gggcgggagt
    21541 ctgtggcgga aaatgtggat cccgtcaata tcctcgcgca ggctctcata ccccttgccc
    21601 gtcggacaga tcacgcagac ctgatatccg gcttgcgcca gcgtggtgga ctccatccac
    21661 acgcgccggt caaacggcac gggaagattc tcaacaacaa caagaatccg gccggccgac
    21721 ttaccaggag attccgtcat aattcacgcc ttccggccgc gccttgaaga gacccaccag
    21781 atccagcacg cgcacggact ttggagcaga cgccaccaca tttctgaatt cggcggtggt
    21841 gttgcatatc accagcagct cggcatgcgc cgcgatttca tccgcatccg cgagcagcgc
    21901 cgacaaatgc gggattttct cctctatata actgcggttt gcgccgatca ggcgggcctc
    21961 ctgcacatgc cggtccataa ttttcagatc gtatcccttt ccgatcagtc gctcggccag
    22021 ttccaccgcg gggctttccc tcaaatcatc ggttcccgcc ttgaaggcca ggcccagcag
    22081 accgattttt cggcagcctg ttcccgcaat ccggtcgagg gtgatgctga tctgctggcg
    22141 gttcgacaca tccagagcgc ccagcatcgg cagccccaga tcgcgggttc ttgcgaaatg
    22201 gttgatggcc cgcagatctt tcggcagaca ggagcctcca taggcaaacc ccggccgcat
    22261 atacacgggg gatacattga gctttgtgtc ctcggcaatg atctccatca ctctgcgggc
    22321 atccacgccc gcggccttgg ccacgcggcc gatttcattg gcaaatgtta tttttgcggc
    22381 gtgccatgca ttcgcggtat acttgatcat ttccgccacg gccacgtccg tcacgatcag
    22441 cggagcgttc accgacgcat acaccctgcg cagaacctcc tcggcatctg catcgtccgt
    22501 tcccaccacg gtgtagggcg gattgtcata gtcccggata gccgttcctt cgcgcagaaa
    22561 ttccggattg aaagccacgc gcacagtctg tcctttggcc ccggcgcgca ggcgctccgc
    22621 acaccgggcg gtggtgccgg gcaggatggt gctgcggatg atcacggtgt gaggctgttt
    22681 ctttgcggca accgccctgc cgagctggtc gcacaccgcc atgacatagc tgtcgtccag
    22741 tgatccatcc gggcggctcg gtgtaccgac acaaaccagc gagacttccg tttgggccac
    22801 ggcccgctcc gcgtcggagg tggccgtgag gcccccgctt ttaacggctt cgtccagaag
    22861 ttcggaaagg cccagctcga tgatgggcga ctggccgcgc gcaatcatgt ccaccttttc
    22921 acggctcacg tcaaccccga caacctgcat gccgtcccgg gccatgcagg cggaagttac
    22981 aactcccacg tatcccattc caaaaacgct aatcttcatt caacacctca atcagggttt
    23041 ccaatgccgc cggcagtctc acatctgccg ccgccgcgag tgtgaacgcc ggttctttct
    23101 tcatgaaacc ggcagagtac cagcccccgt cattctccgc atgcaaaatt ttcggcacaa
    23161 gccccccggg aagcgtgatg cggaggctgc ggccctgccg cattgcaacg gccgtcaggg
    23221 gggaggtcag ttctaccgcg caggccggat gaaaatgaaa gcgcagttca acatgatgcc
    23281 ggcccgagcc cgccagtgtg tctgaaacac gaagccggtt tccgcccagt tccaaagtcc
    23341 gctcatgcac agcgccgctc cggcgatagc cgtcatgtcg gg
//
LOCUS       DAOQWR010000023        23252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2552_length_23252_cov_5.017502, whole genome shotgun sequence.
ACCESSION   DAOQWR010000023 DAOQWR010000000
VERSION     DAOQWR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23252
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2552_length_23252_cov_5.017502"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKK36_03330"
     CDS             <1..670
                     /locus_tag="PKK36_03330"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HNR93624.1"
                     /translation="VFTYVTTDAPVVTYVNTYADVKAETDICCTSGNARAIIESLNSE
                     RIIFIPDRYLAANTARTMGKNIVFPKRTEKGFSFDRIDSGFIGWDGSCEVHERFTVAD
                     VSNARRQFPDVLVLAHPECPESVCQASDYSGGTNGMIKFIQNSKANHILLLTECSMAD
                     NVAAANPDKDLIRICSIRCPHMNLITLEDTLAALQQNRFEITVPEDISRRARKAVERM
                     IAIG"
     gene            680..2098
                     /gene="lpdA"
                     /locus_tag="PKK36_03335"
     CDS             680..2098
                     /gene="lpdA"
                     /locus_tag="PKK36_03335"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNR93625.1"
                     /translation="MADNLKVQLAVVGAGPAGYPAAFRAADLGMSVALIDQRSALGGT
                     CLHEGCIPSKALLHAALIKNEAKSASAFGLYFTEPRLDIDALRIWKNGLVGQLSGGLG
                     MLCKQRAVQFIQGRARFTGPNSILVEQAGEERSVEFDRAIIATGSRPLEIPGLKTESD
                     RIMTARGVLELNRVPESMLVIGGGYIGLELGSVYASLGARVSVAEMTDGLLPGADRDL
                     VRILSRSLEPRFEALMLQTRVAGLTEDNAGLRAKLISAQGDTVEKSFNSILVAAGRRP
                     ATEDLGLENTKVKLDDKGFIITDERRQTGEPSIFAAGDAAGQPMLAHKAMHEALATAE
                     YIATGKGGPFTPRAIPAVVFTDPEIAWCGLTEDQARAENQNIKVGRFPWSASGRAKTL
                     HRADGMTKVIANAESGAILGLAIAGPGAGDLIAEGVLAVESGVTVETLSRVIHPHPTL
                     SETVMEAAEALLGRSVHAARPR"
     gene            complement(2148..6725)
                     /locus_tag="PKK36_03340"
     CDS             complement(2148..6725)
                     /locus_tag="PKK36_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93626.1"
                     /translation="MKMLKTHVYLIAAMLLACPGFLRAAEESFPQVGEVASAAVRKVQ
                     QLQQELSDARTKLQNLSAEHASLKAQAADMQAALDRLEKEKQAAVAREEQAARERRAS
                     MRDAAKAASGRLKDLQAQIEASEKKIADMTGQNQALDKKLSDMSEDLKQKTKSISVLS
                     KERDTLQASVEKMQARLNTVDALEDQVKAVGKEKSLLEKSLKALAQDVEKSQAALAQR
                     DQAMAGMQGEMDSLKEQLGVLASNVKDRDGEIQRAAKEKLALAEQVADLDKQLAAVPG
                     LKEEIEKLKSSNKDLNAQLSASKAESKDLADARAALEKKLTTLPELKDEISGLKSSNK
                     DLNAQLSSSRAEVKTLTDARTALEKTAGELSAQAAALKEELLSRQQELSGLVKAREQL
                     EKDKAELNSALEKTAAEKAAIDAAKAAAEASVKEMDDRIAAVRKELDAALKTNKSLTG
                     QIASLQKKVESLKALEDQIAGLKSANKDLSKQLADAKELGIQAAELEKLTQDQSAAMS
                     GLQQEIRALGQQNQKLAKDLSAAEKKGERANELENANKALADTVAALTAERDMVISQV
                     KSLADSKSSLEGQIEQLGEQVSAARAEAGTRASELEQTVSRLKSEEARASELEKEKTA
                     LAAKLADMDARLAELAVAQDDLKAARKEIAALGKEKSSLSSALEKTEKKLVEAEGAIK
                     PARQKAEALAKDLKVSQNALDALQKAQAETAAKRDALAGELDALKQSRDTLAAELTQT
                     QKDLAQVRSLSSDLEKNATQLAGAKAEMETALSALQAEASAAADALKSEKSRNDALTR
                     ELAGIKKQSEAKEAAMSKLQADFSKSQRDLKAARDQIKATQDDLVQAQKEKDSLSADV
                     RNLTAAVTRLTEEKNRVTADAAGLGVQLAESSKALLAIRSEASNATKSLEALRQERDK
                     LSVELAALQSAQTDLSKTKQDLESALKDATRRLADREKEMASLLTERDALLVKKEELF
                     AQVMDAEKEIRRCSKEIEAVQEVVKQKDARLMSMDSEKAKLEKSLADAAAREAELLPL
                     RDQLNELAKSKDLLEKDCAGLREKLAAYEAEKATLIEAEKLAADNVKKLEEQVRQLSI
                     ERSGLASRMVELQASLEDYAAVKKEVQEVKSRLLDAERRAAQTDRAEETVKNLESRAG
                     QLEASLKQANEALRAANTRIALLEKAEAAAAGKKDSLAAEMQKFKAALDRANTELAET
                     RGKHAEAVAEIASLQQNRSAFEDRMARLNELNTALSAERDRLTAQTGELRSRLAESAE
                     SLDAANATVANLTEANNALKTAADNSAQQEARIKELKSELWELSREVEEAGELRARID
                     SLREQMTGLTRDAAQWQAAATEANAAADDLRRSNTSLQSELGRRDAEIAALRESEQAA
                     SGAAETARAEARTLQARLLETSNALENAEKVADRAAELQTLLSQSEAKISDLNGQAAR
                     ASDRIGVLQKENEKLQKRLEESEQTITRLGEVRNQVDELSRESVRMSRELRKLERAGQ
                     GVDEVTSEELKQRIMQLNSRLETILQDADRE"
     gene            complement(6756..10646)
                     /locus_tag="PKK36_03345"
     CDS             complement(6756..10646)
                     /locus_tag="PKK36_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93627.1"
                     /translation="MNRNTFIAFGLLLALLAAGVPAQAQPAQSAQQLQLNAYSATVAG
                     DRAKGNNDLVAAFEAYERSYNLYKELSESHPDWQKDIVQYRLTYCRDQMDALRPQIAS
                     APASAAKPAPAPAEKAPAKAPAPAARAAAAPSAQSVDFYRTKYSELLEQNEDLREQID
                     SLQAQINSAPVPVADDGAKWRAQHEAVSAQNKELQKRIAQLEQDVRAKSSVSAGGDQQ
                     LRQKILALTDDNQKIQEKLAEAHAEMSALRGSMLGEEQLNMKVTDLDAQLQARGRDVD
                     ALKQENQMIRNDLAAVQSERDGLAGQIEEVNAKWRANLTKVANELKKVRGDAGMLEVE
                     LRKTEARARAAEDQLNQAQQAATAIPALESKVAVAEDLLRKADADIKKLVTEKDQLSA
                     RLADMERVEKEAQARSAQALADAEKREKEALSRSARALSEEQKKADELKSQISDLTAQ
                     LAKKDKELQKQAEDLSMRQKKFEASAQSEYARKEKDLLARVKAAEDQLNQAQQAATAI
                     PALESKVAVAEDLLRKADADIKKLVTEKDQLSARLADMERVEKEAQARSAQALADAEK
                     REKEALSRSARALSEEQKKADELKSQISDLTAQLAKKDKELQKQAEDLSMRQKKFEAS
                     AQSEYARKEKDLLARVKAAEDKEARLNDEMTGLIMASNAMDKRLSAAETERKALLSQR
                     DDMRRQLEEVKSDYLKMAQGSADTAAETAAVKSDLKKAADALAQAEQERDRLKAQVVT
                     LLPQAESAKKLAADLEAARMERDSLQEKYTEAERKAAASSALEEQLKQVQSEKADVEK
                     QYQAELKKAADLQAKTAEAERNARENSDMAASLTAELKQVKQERAALDTELTAQISDL
                     RAKLDQEKARSAGFEKSVQDIRKAGEGDRKRVEALQQEIKAREDTAGRQAAEAEQKIT
                     ALTKDLEGRNAQIEKLTKDLNSVEPRIKEMQDRVQSAELRAEGNARARDALQKGLSGK
                     IEKAEAEAKALRKTVEALQAETAASVKASQDAASDAERLKAEATKLGSQLKTVSDEKA
                     ALQSQLAKLEEQYQATAPLRDRNKVLEKQAGELQKAASQMEKRIASLLNEQTSMQEAA
                     ASSAGAVKELESRLNSLMAENKAVSGERNKLEAQNKSLSDEIAAVKASAAAAADALAA
                     AKADGDKTAGILRQQIADMQSQVDELGKALKKSEGSVKRGEEAVAKSRAMADELKIAQ
                     QRHADAARQAAALSDETKALSAEIAALKKAAAAGEKLQKKAGEENAALQKQLAETAGK
                     LEKTASERTELVEKVKLLEAELREATKVLQELAN"
     gene            complement(10660..11880)
                     /locus_tag="PKK36_03350"
     CDS             complement(10660..11880)
                     /locus_tag="PKK36_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93628.1"
                     /translation="MDNKREKLFAALMAGWILTGCYGAVFAQQISPELNASASRYSRA
                     ETLDLQIEIERLRKAFEFFREDRELALEELSQLERRNTRLQEQVDKLEAALKKKEKRE
                     KPGGLIGKLMAPESGGDELDALKEELNRERRMRRHYEAQVREMDLALAKFRQERVGVA
                     ESIPEMTEPVPPPEAVKPSRKEPPAEKPSTVVRTAAAEPFPAPAVREEKIQPVPVEEK
                     AEAARDEDALMANGEKLLGEGRVEEALAVFSVLLEQNPSNARAMMGLASCYYNIWDFK
                     NAESILNQLLDIHPTYAEAMGLKGMISWRNNDLEKADRYLSRGLLLDAKSAQLHNYMG
                     IVHHSRQKLQEAAKEFRQAVELDPRHAEAQFNLAVVLATSSPSDITSARKYYESAISL
                     GSERNQDLEKILYP"
     gene            complement(12043..13119)
                     /gene="amrS"
                     /locus_tag="PKK36_03355"
     CDS             complement(12043..13119)
                     /gene="amrS"
                     /locus_tag="PKK36_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNR93629.1"
                     /translation="MHTGDTAYTARHWKKLPDGRVECELCPHRCRLKDGQRGLCFVRA
                     AKKGELVLTTYGRSSGFCIDPIEKKPLFHYLPGTPVLSFGTAGCNLQCRMCQNWSMSK
                     ATEMQILAEDASPSLIAQAAKRQGCRSVAFTYNEPLIFLEYAVDVARACHEEGIKTVA
                     VTAGYVNPGAREDFFAHMDAVNMDLKGFTDEFYRRICGGDLQTVLETMIYLRQKTSAW
                     VEITNLVIPGLNDSPEEIRAMVCWLRAEMGADVPLHFSAFHPSYKMQDKPPTPPETLI
                     MARNIAREEGLSYVYVGNVHDPEGGSTLCTGCGRKIIGRDWFVLTSWDLTPDGKCRHC
                     GTACPGVFDAKPGTWGARCVPVQL"
     gene            13227..14012
                     /gene="amrB"
                     /locus_tag="PKK36_03360"
     CDS             13227..14012
                     /gene="amrB"
                     /locus_tag="PKK36_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNR93630.1"
                     /translation="MKKTRQPAVAGMFYPDKPEVLRRMTEQYLAEARPSGPVPRAMIA
                     PHAGYPYSGPVAASAYARLKPAAETIREVVLFGPSHRVLFGGVALSSAEAYATPLGSV
                     PLTHAPAQSLAGLSQVGILDEAHRPEHSLEVHLPFLQIVLKSFDLFPVVIGDTTPAAV
                     AEVIRRLWAGPERLFIISSDLSHYLPYEQAVETDRQTTAAIENLDAEGIEEEQACGRI
                     GICGMLMVAREKNLKVETVDLRNSGDTAGPRDQVVGYGSYVFT"
     gene            13999..14580
                     /gene="amrA"
                     /locus_tag="PKK36_03365"
     CDS             13999..14580
                     /gene="amrA"
                     /locus_tag="PKK36_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNR93631.1"
                     /translation="MSSHDSGPGLTPHEQQLLLSIARCSIEYGLLQKRPLLPAESDMP
                     PALTRIQATFVTLTLKENLRGCIGMLEAVYPLCRDVAQNAFAAAFRDPRFPPLSEKEL
                     PVVKMEISLLTPPVPFPVSSEQDLLSRMRPGVDGLILEDGFRRGTFLPAVWEDLPDPR
                     DFLMHLKLKAGLPPDYWSDTLRVSRYTAHKISE"
     gene            complement(14585..15166)
                     /locus_tag="PKK36_03370"
     CDS             complement(14585..15166)
                     /locus_tag="PKK36_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93632.1"
                     /translation="MLRANQPPAARKPGRAGPVLAIILGLLLIAGTVTVTAVWGWQLY
                     ARQQMVYGDLRVLIRAGEGFFREYGIWPTPHSGQYGDFRYGRDFPNAYVMNILRAVSG
                     DGNAGHSVNPGKIVFLEVRPAAPGVSGLNDAGSLLDPWGQPYQIVLDTDLDNSCQIEN
                     SIYGTLPGYGMVVWSCGPDGISDTEDDLCSWPR"
     gene            complement(15176..16438)
                     /locus_tag="PKK36_03375"
     CDS             complement(15176..16438)
                     /locus_tag="PKK36_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR93633.1"
                     /translation="MARFKYTALDSRGQEVEGVIDAENETSALSKIREKNLFPSQVSE
                     VGRAMPKKGGGKAASGKGLKMELKMPSFLIRVKPKQLMVSTRQLATLVNAGLPLLRGL
                     QVMQRQEKNPALKKALSEIADAIQSGSTFAEALGQHPKIYNRLYVNMAKAGELGGVLD
                     QVLVRLAEFMEKAQAIKSKVISAMIYPVVVLCMAGGILAFLMIFIIPKFEQIFQELLE
                     GEGLPPLTQFVLNVSRAFTHHIAWVVVGVVILIVLVRIAAKTAWGRYSLDRTKIAAPV
                     FGTLVKKTAIARFSRTLGTLMTSGVPVLQALNIVRDTAGNEVIARAVNQIHDSVKEGE
                     NMAPPIEASGIFPPMVISMVEVGEETGGLPEMLMRIADTYDGEVDDTVAGLTSIIEPI
                     LIIFLAVVVGTIVIALFMPLISIIGKLS"
     gene            complement(16460..18172)
                     /locus_tag="PKK36_03380"
     CDS             complement(16460..18172)
                     /locus_tag="PKK36_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR93634.1"
                     /translation="MSDLQYKDLLLQHLVEEGVLTQTQAADVVDEHERTGKMVRDIIV
                     DMSILSRDEILEHVASLLGCEVINLMATDIPQRVVKSIPASVARMYNVIPVEADEQSV
                     VLATSELLSPEVVDELTFVLTRDISFVVAREEDINSYLAKFYGDESDSVTDMLSALES
                     ELEGTETLVEFGEQEGKVEELEHAANLTPVVRFVNLVLYQAVRDRASDIHFEPFETEF
                     KIRYRVDGALYEMAPPPKHLALPVTSRLKVLSGLNIAERRLPQDGRIQLNIGGRAIDF
                     RVSTLPTQFGESVVLRVLDRSVVSLDLENIGMPEDIYNDFSADIKKPNGIIIVTGPTG
                     SGKTTTLYSALRRLNIVDSKLMTAEEPVEYDVEGLIQVPVDEAIGVTFVRVLRAFLRQ
                     DPDIIMVGEIRDMETAEIAIQASLTGHLVFSTLHTNDAAGAVTRLIDMGVEPFLISST
                     LEAVLGQRLVRTICSTCKTPYQPDADILDQLGLKPEIIRDTPFYFGEGCSHCSQTGYR
                     GRRGIYEYLRVSDPVRGLINQRKPTIVIRDKAIELGMRTLREDGIRCILDGSTTVEEV
                     LKYT"
     gene            complement(18182..19279)
                     /locus_tag="PKK36_03385"
     CDS             complement(18182..19279)
                     /locus_tag="PKK36_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNR93635.1"
                     /translation="MTMASSDIKISDLLQLVVDEGASDLHLAVGAPPILRLHGRLHPL
                     DAEPLRPIDTERFMKSITSEDHQQKIREGGGTDFGFGFGTAARFRVSVLKQKGHLGMV
                     LRQIPTNLMSLEEIGLPPQVKELLYKPRGLVLVTGPTGSGKTTTLASMINVINIERDC
                     HIITIEDPIEYYHDHKRSIITQREIGVDVPSFEEALRRGLRQDPDVMLVGEMRDLETM
                     EAAISAAETGHLVFATLHTTGAARTVDRIVDAFPTNQQEQIRTQLASSIVAVISQLLL
                     VRHDAPGRIAAFEIMIATPSIRSLIRDNKTFRITSDIQTGARWGMVTLDAHLESLYRE
                     GSISYEDLITKSQDPEALMEKIEAAGGKKKK"
     gene            complement(19276..20985)
                     /gene="gspE"
                     /locus_tag="PKK36_03390"
     CDS             complement(19276..20985)
                     /gene="gspE"
                     /locus_tag="PKK36_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNR93636.1"
                     /translation="MTANDEYIVEILESVGLITRAQAEEALTAAREEDKDVQDVLVEK
                     EMASKLDILKTLAYQFGMETVSLANMEVTQEVIDFVPNKIARRYKIVPVYKNENTLTV
                     ALSDPLDVETLDSLRYLLKCNVEGVVAPAEEIEVALNHYYAHTPETVETMLEEITEGT
                     VAVPTGAEILAEDGEATEADAPIIKLVSLIILEAFRSRASDIHLEPLARQFRVRYRID
                     GVLHEVESPPKRLQSAIISRVKIMANMKIAEKRVPQDGRIQVNVMGRDLDLRVSSIPT
                     NHGESIVMRILDKQGLMLGLPELGFLADDQKVFERLIGLPDGILLVTGPTGSGKTTTL
                     YGCLNEINRPDRKIITVEDPVEYQLSGINQVHVRSDIGLTFASALRSILRQAPNIIMI
                     GEIRDIETAEIAVNASLTGHLVFSTLHTNDAPSAITRLIDIGVKPFLVASSTRAIMAQ
                     RLVRKICPKCKEEYKPSEAEYRLLGPAAAQLREAQLFHGRGCNECKLTGYRGRLGIFE
                     IFVINDEVRHMIYEQTSAAELRQRARELGMRTLREDGLRKVISGTTTMEEVLRVTMGD
                     SNL"
     gene            complement(21174..21524)
                     /locus_tag="PKK36_03395"
     CDS             complement(21174..21524)
                     /locus_tag="PKK36_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR93637.1"
                     /translation="MDSHLFDILIEEQGDVKIACVNGPVDVSNIDIFSEKVGALCRGT
                     SNKTVLDLSGMNYINSRGMGLMIEFSRQAALGGGRFVVCSPRPVILKNIELLRLDAVV
                     EIFETRADAVAALS"
     gene            complement(21545..22312)
                     /locus_tag="PKK36_03400"
     CDS             complement(21545..22312)
                     /locus_tag="PKK36_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HNR93638.1"
                     /translation="MKQPVRIMPCLDMQNGRVVKGVHFVDIKDAGDPVACAKAYCAAG
                     ADELAMLDITATIENRGTLLDVVRRVADVTTIPFTVGGGISDVKSAGDVLKAGANKIS
                     TSSAAFRKPQVIREMIKEFGADKVTVAIDVDQNPKLPSGYEVYIDGGRTATGKDAVEW
                     AKEVDGYGVKVILPTSKAGDGARTGYDLPVIRAIRNAVKAEVVASGGAGEMKHFLEAA
                     EAGATILLAASVFHFGIISIPDLKQYLKTSGVMLAQA"
     gene            complement(22729..>23252)
                     /locus_tag="PKK36_03405"
     CDS             complement(22729..>23252)
                     /locus_tag="PKK36_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR93639.1"
                     /translation="DGQGDLKKTVGPEDVFYYIYAVLHSPTYRTRYADFLKIDFPRVP
                     LTSDKKLFAALVKLGGELTALHLMESPELNKPATSFPVAGDNIVAKGFPKFKDGRVYI
                     NKTQYFDNVPSNVWNFTIGGYQVCEKWLKDRRERVLTNDDLQHYKKIVAALGRTIELM
                     REIDQAIPKWPME"
BASE COUNT         4159 a         7263 c         6402 g         5428 t
ORIGIN      
        1 ggtgtttaca tacgtcacca ccgacgcgcc ggtggtgacg tatgtaaaca cctatgccga
       61 tgtgaaggcg gaaacggaca tttgctgcac ttccggcaac gcccgcgcca tcattgaatc
      121 tttgaacagc gagcggatca ttttcattcc tgaccgctac ctggcggcca acaccgcgcg
      181 cacgatgggg aaaaatattg tattcccaaa gcgcaccgag aaaggattct cttttgaccg
      241 gattgattcc ggctttatcg gctgggacgg ttcctgcgaa gtgcacgagc gctttacggt
      301 ggcggatgtc agcaatgcgc gccggcagtt tcccgatgtg ctggtactcg cccaccccga
      361 atgtcccgaa tctgtctgcc aggcttccga ttactcgggc gggacgaatg gaatgattaa
      421 atttatccag aattccaagg caaaccacat tctactcctg acagaatgct ccatggctga
      481 caacgtcgcg gcggccaatc cggacaagga cttgatcagg atttgctcca tccgctgtcc
      541 gcacatgaat ctgatcaccc tcgaggacac ccttgccgcg ctgcagcaga accgtttcga
      601 aatcaccgtt ccggaagaca tcagccggcg ggcccgcaag gcggtcgagc gcatgatcgc
      661 catcggatag gagtttatta tggcagacaa tctcaaggtg cagcttgccg tggtcggcgc
      721 cggcccggct ggatatcccg ccgcgttccg tgcggcggac ctgggcatga gcgttgccct
      781 gattgaccag agatccgctc tcgggggaac ctgcctgcat gaaggctgta ttccctccaa
      841 agcccttctg catgcggccc tgatcaagaa tgaggccaaa agcgcatcgg cattcgggct
      901 ttatttcacg gaaccccggc tggacattga tgcccttcgc atatggaaaa atggactcgt
      961 cggccagctt tccggggggc tcggcatgct ctgcaaacag cgggccgtgc agttcattca
     1021 gggccgcgcg cgcttcactg gtccaaattc aatccttgtg gaacaggccg gcgaagagcg
     1081 gtcagttgaa ttcgacaggg cgatcatcgc caccggctcg cggcctcttg aaattcccgg
     1141 actaaagacg gaatccgacc gtattatgac ggcgcgcggc gtgctggagc tcaatcgcgt
     1201 tccggaatcc atgctggtca tcggcggcgg atacatcggc cttgaactgg ggtccgttta
     1261 cgcctcgctg ggcgcgcgtg tcagcgtggc agaaatgacg gatggactcc ttcccggagc
     1321 cgaccgcgac cttgtgcgca tcctcagccg cagcctggaa ccccgatttg aggccctcat
     1381 gcttcaaacg cgcgtggcag gcctaactga agataacgca ggcctgcgcg caaaactcat
     1441 ttctgcgcag ggtgacactg ttgaaaaaag cttcaacagt atccttgtgg ccgccggacg
     1501 aaggccggcc acagaggatc tcggcctgga aaacacaaag gttaaactgg atgacaaggg
     1561 cttcatcata accgatgaac gccgccaaac cggggagccc tccatttttg cggccggaga
     1621 cgcggccggc cagcccatgc tggcgcacaa ggccatgcat gaggccctcg cgacagccga
     1681 gtacattgcg accggcaagg gcggcccgtt tacgccccgc gccattcctg ccgtggtgtt
     1741 caccgatccc gaaattgcat ggtgcggcct gaccgaagat caggcccgcg cggagaatca
     1801 gaacatcaaa gtcggccgct tcccctggag cgcctcgggc cgggccaaga cgcttcatcg
     1861 ggctgacggt atgaccaaag tcatcgcgaa tgctgaaagc ggagcgatcc tgggccttgc
     1921 catcgcgggg cccggagcag gcgatcttat tgccgagggt gtgctggccg ttgaatcagg
     1981 tgtcaccgtc gaaacgctca gtcgggtcat tcacccccac cccacgttgt cagaaaccgt
     2041 tatggaagcc gcggaagccc tgctgggccg aagtgttcat gccgcccggc cgcgataaag
     2101 aaaaagccgg ccgcgtgaca cggccggcct ttccggaaag aaatccgcta ttccctgtcg
     2161 gcatcctgaa ggatggtttc caggcgtgag ttcaactgca taatgcgctg cttgagttcc
     2221 tccgatgtaa cctcatcaac cccctgcccg gcgcgctcca gcttgcgaag ctcgcggctc
     2281 attcttacgc tttcacggga gagttcatcc acctgattgc ggacttctcc cagacgggta
     2341 atggtctgtt ccgactcttc cagcctcttc tgcagcttct cgttttcctt ctggagcaca
     2401 ccgatacggt ccgaggcccg tgcggcctga ccgttgagat ccgaaatctt cgcttcagac
     2461 tggctcagca gagtctgcag ttcagcggcg cgatccgcca ccttctccgc attttcaagg
     2521 gcattggatg tttcgagcag ccttgcctgg agggttcgcg cttcagctct agccgtctca
     2581 gccgcaccgg aagcggcctg ttcagactca cgaagcgcgg caatctccgc atcacgccgg
     2641 cccagttccg actgcagcga tgtgttgctg cggcgcagat catcggccgc cgcattggct
     2701 tccgtggcgg cggcctgcca ctgcgcggca tcccgggtca gtccagtcat ctgctctctc
     2761 agactgtcaa tccgggcacg cagttcgccg gcttcctcaa cctcgcgcga cagctcccaa
     2821 agctcgcttt tcagctcctt gatgcgcgcc tcctgctgcg cgctgttgtc cgctgcggtc
     2881 ttcagggcat tgttggcctc cgtaaggttc gccacagtgg catttgcggc atccaaagac
     2941 tccgcggatt cggccaggcg gcttctcagt tctccggtct gggccgtcag ccggtcgcgt
     3001 tccgccgaca gggccgtatt cagctcattg agcctggcca tgcggtcttc aaacgcgctc
     3061 ctgttctgct gaagagacgc aatctctgca accgcttccg catgttttcc gcgggtctct
     3121 gccagctcag tatttgccct gtccagcgct gccttgaatt tctgcatttc ggcggcgagg
     3181 ctgtctttct ttccggcggc ggcggcttcc gccttctcaa gcagcgcaat ccgtgtattg
     3241 gccgcgcgca gcgcctcatt ggcctgtttc agagaggctt caagctgtcc tgcgcgagac
     3301 tcgagattct tcactgtttc ttcagcccgg tccgtctgcg ccgccctgcg ctccgcatcc
     3361 agaagccggc tcttaacctc ctgcacctct tttttcaccg cggcataatc ttccagggac
     3421 gcctgcagtt caaccatccg ggaagccagt ccggagcgct cgatggacag ctggcgcacc
     3481 tgctcctcca gcttcttcac attgtcggca gcaagcttct ccgcctcgat cagagtggcc
     3541 ttctccgctt cataagccgc cagtttctcc cggaggcctg cgcagtcctt ctccagaaga
     3601 tcctttgatt tcgcgagttc attcagctga tcccgaagcg ggagaagttc cgcctcgcgg
     3661 gcagcggcat cggccaggct tttttccagt ttcgcttttt cactgtccat ggacatcagc
     3721 cgggcatcct tctgtttgac aacctcctgc acggcctcga tttccttcga acagcggcga
     3781 atttccttct cggcatccat cacctgcgca aagagttctt ctttcttgac cagcagggca
     3841 tcccgctccg tcagcagaga ggccatctct ttttccctgt ctgccagccg gcgagtcgca
     3901 tcctttaagg cggattccag atcctgttta gttttcgaga gatccgtctg cgcgctctgg
     3961 agcgcggcaa gctccacgct cagcttgtcc ctttcctgcc ggagcgcttc aagcgatttg
     4021 gtcgcattcg acgcttcaga acggatcgcc agcagggcct ttgaactttc cgcaagctgc
     4081 acccccagac cggcggcatc ggccgtcacg cggtttttct cttcggtcag ccgcgtgacc
     4141 gcagcggtca gattccggac atcggcagag agactatcct tttccttctg cgcctgaacc
     4201 agatcatcct gtgtcgcctt aatctgatcc cgggcagcct tcaaatcccg ctgggatttg
     4261 ctgaaatccg cctgcagttt gctcatggcg gcttcctttg cctccgactg cttcttaatt
     4321 cccgccagtt cgcgggtcag cgcatcgttg cggctctttt cagacttcag cgcatccgca
     4381 gcagcggatg cttcagcctg aagcgcagac agagcggttt ccatttccgc tttggctccg
     4441 gcaagctgtg tcgcgttctt ttccagatcg gaggaaagac tgcggacctg cgccagatct
     4501 ttctgggtct gcgtgagttc ggctgccagt gtgtccctgc tctgcttcag cgcatccagt
     4561 tcgcccgcca gcgcatctct tttggcggct gtttcggcct gtgccttctg cagggcatcc
     4621 agcgcgttct gcgacacctt caaatctttc gccagtgcct cagccttctg gcgtgccggc
     4681 ttgatggcgc cctctgcctc aaccagcttc ttctcggttt tttcaagggc cgaggaaagg
     4741 ctcgatttct ccttcccgag cgcggcaatt tctttgcgcg cggccttcaa atcatcctga
     4801 gcgacggcaa gttcagccag ccgggcgtcc atgtcagcca gtttggccgc aagggctgtc
     4861 ttttcctttt caagttccga cgcgcgggcc tcttcggact tcaaccggga taccgtctgc
     4921 tcgagctcag aggcacgagt tccagcctcc gcgcgagccg ccgaaacctg ttcccccagc
     4981 tgttcgatct gcccttccag cgaacttttt gaatcagcaa ggctcttgac ctgcgatatg
     5041 accatatccc gttcggccgt cagtgcggca accgtatctg caagcgcctt gtttgcattc
     5101 tccagttcat ttgcgcgctc gcccttcttt tctgccgcgg agagatcttt tgcaagtttc
     5161 tgattctgct gtcccagagc cctgatttcc tgctgcagcc cggacatggc ggcagactga
     5221 tcctgtgtga gcttttccag ttcggcggcc tgaatcccaa gctcttttgc atccgcgagc
     5281 tgctttgaaa ggtctttgtt ggcggatttc agcccggcaa tctgatcttc cagcgccttg
     5341 aggctttcca cttttttctg caaagaagca atctgtccgg tcagagactt gttcgtcttc
     5401 agcgccgcat ccagttcctt tctgacagcg gcaatccggt catccatttc cttcacagag
     5461 gcttctgctg cggctttggc tgcatcaatt gcggcttttt cggccgccgt tttctccaaa
     5521 gcggagttca gttccgcctt gtccttttcc agctgctcac gggcttttac cagccccgaa
     5581 agttcctgct ggcgggacag caattcctcc ttaagcgccg ccgcctgcgc agacagctct
     5641 ccagcggtct tttccagcgc ggttcgggca tcggtcagcg ttttcacctc cgcccgggaa
     5701 gaagacagct gcgcattcag gtccttgttc gaggacttca gccccgaaat ttcatccttc
     5761 agttccggaa gggtcgtcag cttcttttcc agcgcggccc gggcatcagc cagatccttt
     5821 gactccgcct tcgatgcaga gagctgcgcg ttcagatcct tgttggaaga tttcagcttc
     5881 tcgatttcct ccttaaggcc cgggactgcg gcaagctgct tatccaaatc agccacctgt
     5941 tctgcaagcg ccagcttctc ctttgccgcg cgctggattt cgccgtcgcg gtccttcacg
     6001 ttcgaagcca ggacccccag ctgttctttc agagaatcca tttccccctg cattcctgcc
     6061 atcgcctgat cccgctgcgc cagggcggcc tgggattttt ccacgtcctg cgccagcgcc
     6121 ttcaacgact tctccagcaa ggacttttcc ttccccacag ccttgacctg gtcctcaagg
     6181 gcatccaccg tattcaaccg ggcctgcatt ttttcaacac tggcctgcag cgtgtctctt
     6241 tctttggaga gcaccgatat gcttttagtt ttctgcttga ggtcttccga catatccgac
     6301 agctttttgt ccagcgcctg attctgtccg gtcatatccg caattttctt ttctgacgcc
     6361 tcaatctgag cctgcagatc cttcaggcgg cccgaagccg cctttgccgc gtccctcatg
     6421 cttgcgcgcc gctcacgggc cgcctgttcc tcgcgggcca cggccgcctg cttttccttt
     6481 tcaaggcggt cgagggcggc ctgcatatcc gccgcctgtg ctttcaggga ggcatgctcc
     6541 gccgacaggt tttgaagctt agtcctcgca tcggaaagct cctgctggag ctgctgaacc
     6601 ttgcgcaccg ccgcactggc cacctctccc acctgcggga aagattcttc ggccgcgcgc
     6661 agaaaacccg ggcatgccag cagcatcgcg gcgatcagat atacatgtgt cttaagcatc
     6721 ttcataataa acctctcaac atctttcctg cgcggctagt tggccagttc ctgcaggact
     6781 tttgtggcct cgcgaagttc cgcttccagg agtttgactt tttcaaccag ctcggtgcgt
     6841 tcggatgctg tcttttccag cttcccggcg gtttctgcca gttgtttctg cagcgccgca
     6901 ttctcttctc cggccttctt ctgaagtttc tctccggcgg cggccgcttt cttcagcgcg
     6961 gcgatttcag cggacagggc ttttgtttcg tcactcagcg cggcagcctg cctggcggcg
     7021 tccgcatggc gctgctgggc aattttcagc tcgtccgcca tggccctgga ttttgccacg
     7081 gcttcttcgc cgcgcttgac ggagccttcc gatttcttga gcgctttgcc aagctcatcc
     7141 acctggctct gcatgtctgc aatctgctgg cggagaatcc ccgccgtttt atctccatcg
     7201 gcctttgcag cggcgagggc gtctgcggcg gcggccgcag aagccttgac tgccgcaatt
     7261 tcatcggaca ggctcttgtt ttgagcctcc aatttattcc gttcaccgga aacagccttg
     7321 ttttctgcca tcaggctgtt gaggcgcgat tcaagctcct tgaccgcacc ggccgaagag
     7381 gcggcggctt cctgcatgga ggtctgttca ttcagcagcg aggcaatgcg cttctccatt
     7441 tgggacgcgg ccttctgaag ttcgcccgcc tgtttctcaa gaaccttgtt tcggtcgcgc
     7501 agaggggctg tggcctgata ctgctcttcc agtttggcca gctgcgactg cagtgcggcc
     7561 ttttcatccg aaaccgtttt cagctgagaa ccaagttttg tcgcctcggc tttcaggcgt
     7621 tctgcatcag aagcagcgtc ctgcgaagct ttcaccgacg cggcggtttc agcctgcaga
     7681 gcctcaacag tcttccggag ggctttggct tccgcctccg ccttttctat cttcccggag
     7741 agtcccttct ggagcgcatc gcgggcacgg gcattgcctt cggcccgcag ttcggcgctc
     7801 tgcacgcgat cctgcatttc tttgattcga ggctccaccg aattcaaatc tttggtcagc
     7861 ttttcaatct gcgcgtttct gccttcaagg tcctttgtga gcgcggtgat cttctgctca
     7921 gcctcggcgg cctgccggcc cgccgtgtct tcgcgggcct tgatttcctg ctggagagcc
     7981 tccacgcgct tgcggtcgcc ctcacccgcc ttgcggatat cctgcaccga cttctcaaag
     8041 ccggcggacc gggccttttc ctggtccagt tttgcgcgca aatcactgat ttgggctgtc
     8101 agttccgtat ccagcgcggc acgctcctgc ttcacctgct tgagttccgc cgtcaggctt
     8161 gccgccatat ccgaattttc gcgggcattg cgctctgcct ccgcggtctt tgcctgcaga
     8221 tcggcggcct ttttcagctc ggcctgatac tgtttctcca catcggcctt ttctgactgc
     8281 acctgtttga gctgctcttc aagcgcagaa gatgccgcgg ccttccgctc ggcctcggtg
     8341 tatttttcct ggagagaatc cctctccatg cgggcagctt ccaggtcggc cgcaagcttc
     8401 tttgcggact cggcctgtgg gagcagcgtc accacctggg ctttcagccg gtcgcgctcc
     8461 tgttccgcct gggccagcgc atcagccgcc tttttcaaat cagatttgac tgccgcagtt
     8521 tcagccgcag tatcagcgga accctgggcc attttcagat agtcgctctt aacttcttcc
     8581 agctggcggc gcatatcatc acgctggctc agcagcgcct tgcgttcggt ttccgccgcg
     8641 gacagccgct tgtccatcgc attggaagcc atgatcagcc cggtcatctc atcattcagc
     8701 cgggcttcct tgtcctcagc cgctttcacg cgggccagca gatccttttc cttcctcgcg
     8761 tattcagact gcgcggatgc ctcgaatttt ttctgccgca tactcaagtc ttctgcctgt
     8821 ttctgaagtt ccttgtcctt tttggcaagc tgtgcagtca aatcggaaat ctgcgacttc
     8881 aactcatccg cttttttctg ctcttcgctc agtgccctgg cgctgcgcga cagggcttct
     8941 ttttcacgct tttccgcatc ggcaagcgcc tgagcgcttc gggcctgggc ctctttttca
     9001 actctctcca tatcggccag ccgggcggaa agctgatctt tttccgtcac gagctttttg
     9061 atgtcggcat cggccttgcg cagaagatct tcggccaccg ccaccttgga ttccagagcc
     9121 ggaatcgccg tcgcggcctg ctgggcctga ttcagctggt cctcagccgc tttcacgcgg
     9181 gccagcagat ccttttcctt cctcgcgtat tcagactgcg cggatgcctc gaattttttc
     9241 tgccgcatac tcaagtcttc tgcctgtttc tgaagttcct tgtccttttt ggcaagctgt
     9301 gcagtcaaat cggaaatctg cgacttcaac tcatccgctt ttttctgctc ttcgctcagt
     9361 gccctggcgc tgcgcgacag ggcttctttt tcacgctttt ccgcatcggc aagcgcctga
     9421 gcgcttcggg cctgggcctc tttttcaact ctctccatat cggccagccg ggcggaaagc
     9481 tgatcttttt ccgtcacgag ctttttgatg tcggcatcgg ccttgcgcag aagatcttcg
     9541 gccaccgcca ccttggattc cagagccgga atcgccgtcg cggcctgctg ggcctgattc
     9601 agctggtcct cagccgctct ggcacgggcc tccgttttcc gcagttccac ttcaagcatc
     9661 ccggcatcgc cccggacttt cttaagctcg ttggccacct tggtcagatt ggcccgccat
     9721 ttggcgttca cttcctcaat ttgtccggca agaccgtccc gttcgctctg gacagcagcc
     9781 agatcattgc ggatcatctg attttcctgc ttcagcgcat ccacgtcgcg tcccctcgcc
     9841 tgcagctgcg cgtccagatc agtgaccttc atgttcagct gttcttcgcc gagcatggac
     9901 ccccgcaggg cgctcatttc cgcatgggct tcggccagct tctcctgaat tttctggttg
     9961 tcatcggtca gggccaggat cttctgcctc aactgctggt cgccgccggc cgaaacgctg
    10021 ctcttcgcgc gaacgtcctg ctccagctgg gcgatccgct tctgaagctc tttgttctgg
    10081 gccgagacgg cctcatgctg cgcgcgccac ttcgcgccat catccgcgac gggaacaggt
    10141 gcgctgttga tctgagcctg cagagaatca atctgctcgc gcaggtcctc attctgctcc
    10201 agcagttctg aatacttggt ccgatagaaa tccacggact gtgcggaggg agccgccgcc
    10261 gcacgggcgg ccggagccgg ggcttttgca ggcgcctttt ctgccggggc aggagcaggt
    10321 ttcgccgccg aagccggcgc cgaggcaatc tgcgggcgca gggcatccat ctggtcacga
    10381 cagtacgtca gccggtactg gacgatgtcc ttctgccagt cgggatgcga ttcgctcagc
    10441 tccttataca ggttgtacga gcgctcatag gcctcgaacg ccgcgaccag atcgttgttg
    10501 ccctttgccc tgtcgcccgc cacggtggcg gaataggcgt tcagctgaag ctgctgtgcg
    10561 gactgtgcag gctgcgcctg tgcaggcacg ccggccgcaa gcagggccag cagcagtcca
    10621 aatgcgataa atgtgtttct gttcattggt tcgactcctc tatggataga gtattttttc
    10681 aagatcctga tttctttcgc tgcccagact gattgcactc tcataatatt ttcgggcgga
    10741 cgtgatgtcc gaaggactcg atgtggccag cacaacggcc agattgaact gtgcctccgc
    10801 atgccgggga tcgagctcca cggcctgcct gaattccttt gcggcctcct ggagtttctg
    10861 gcgggaatga tgaacgattc ccatgtaatt atgaagctgc gcgctctttg catccagcag
    10921 aagcccgcgg gacagatacc ggtccgcctt ctccagatcg ttgttgcgcc agctgatcat
    10981 ccccttgagg cccatcgctt cggcataggt cggatggata tccagcagct ggttcagaat
    11041 gctttcggca tttttgaaat cccagatgtt gtagtagcag gaagccagcc ccatcattgc
    11101 gcgcgcgttg gagggattct gttccagcag aactgaaaac acggccagcg cctcttccac
    11161 acgaccttcc cccagaagct tctcgccatt ggccatgagc gcatcctcat cgcgcgccgc
    11221 ctccgccttc tcctcgacag gtaccggctg gattttctcc tctctcacag ccggggccgg
    11281 gaacggttcg gcggccgccg tgcgaacaac cgtggagggt ttctccgcgg ggggctcttt
    11341 ccgcgagggc ttcaccgcct ctggaggcgg aacaggctct gtcatctcgg gaatgctctc
    11401 ggccacgccc acgcgttcct gcctgaattt ggccagcgcc agatccattt cgcgcacctg
    11461 ggcctcgtaa tgcctcctca tgcggcgttc gcggttcagt tcctccttca gggcatccag
    11521 ctcgtccccc ccggactccg gcgccatcag tttgcctatc aaccctccgg gtttctcacg
    11581 cttctctttt ttcttcagcg cggcttccag tttatcaacc tgctcctgca ggcgggtgtt
    11641 tcggcgctca agctgggaca gctcctcaag ggcaagttcg cggtcctccc ggaaaaattc
    11701 gaaggccttg cgcagccgct cgatttctat ttgaagatca agagtttccg cgcgggaata
    11761 gcgcgaggcg ctggcattca gttctggaga tatctgctgg gcaaaaaccg ccccgtaaca
    11821 ccccgtcaga atccatccag ccatcagtgc ggcaaacagt ttttctcgtt tattatccat
    11881 atacggcacc tgtttcctta cagcccagac atttagccgt cttttatagc ttttcgcatc
    11941 gcaggcaggc aagcgggaaa gttccaatca ttggaagttt ttacgcaaaa agttccaatg
    12001 attggaagtt ttttgggaga aagttccagg ggttggaagt tttcagagct gcaccgggac
    12061 gcagcgcgcg ccccacgtgc cgggtttggc gtcaaacaca cccggacagg ctgttccgca
    12121 gtgccggcat ttcccgtcag gcgtcaaatc ccaggaggtc agcacaaacc agtcgcggcc
    12181 gatgatcttc cggccgcagc ccgtgcagag cgtgcttccg ccttccgggt cgtgcacgtt
    12241 gccgacatag acatagctca acccctcttc ccgggcaata ttccttgcca tgatgagcgt
    12301 ttccggcggc gtgggcggtt tgtcctgcat tttatacgac ggatgaaagg cgctgaagtg
    12361 cagcgggaca tccgcgccca tctccgcgcg aagccagcag accatggccc ggatttcttc
    12421 cggactgtcg ttcagcccgg gtatgaccag attggtgatc tccacccacg ccgatgtctt
    12481 ctgacgcagg tatatcatgg tttccagaac agtttgaaga tcccctccgc aaatgcgcct
    12541 gtaaaattca tcggtgaatc ccttcaaatc catgttcacc gcatccatgt gcgcaaagaa
    12601 atcctcccgc gcaccggggt tcacataacc cgcagtcacc gcgaccgttt ttatcccttc
    12661 ttcatggcag gctctggcca catccacggc atattccaga aagatcagcg gctcgttata
    12721 ggtaaaagcc acgctccggc agccctgccg cttcgccgcc tgcgctatga gcgatggcga
    12781 agcatcctcg gccagaatct gcatttccgt ggccttggac atgctccagt tctggcacat
    12841 ccggcactgc aggttgcagc cggccgttcc gaaggacaga accggcgttc cgggcagata
    12901 atgaaacagc ggcttctttt ctatgggatc aatgcagaaa ccgcttgaac ggccgtaggt
    12961 cgtgaggacc agttccccct tcttcgccgc acgaacaaag cacagccccc gctggccatc
    13021 cttcagccgg cagcggtgcg gacagagttc acactccact ctcccgtccg ggagcttctt
    13081 ccagtgtctc gcggtatatg ctgtgtcgcc tgtgtgcatg ctcttctgct gcccacattc
    13141 tactgccgca aaacgaactc cacaaggggc aacctgcttg ctttttcggt caaacggggt
    13201 atatcatgcg gatggaagga gagagcatga agaagacgcg ccagcctgcc gttgccggaa
    13261 tgttttatcc ggacaagccg gaagtccttc gcaggatgac agagcagtat ctggccgagg
    13321 cccggccttc aggccccgtt ccccgcgcca tgatcgcgcc ccacgcgggt tatccctatt
    13381 ccggccccgt ggccgcttcg gcttatgcaa ggctgaaacc cgccgccgaa accatccggg
    13441 aggtggtgct gttcggcccg tcccacagag tgcttttcgg cggcgtcgcc ctgagcagcg
    13501 cggaggccta cgccacgccg ctcggcagcg tcccgctgac ccatgccccg gctcagtccc
    13561 ttgccggtct cagccaggtc ggcattctgg acgaggccca tcgtccggaa cacagcctgg
    13621 aagtccacct gccgtttctt cagattgttc tgaagtcatt cgacctgttc cccgtcgtca
    13681 tcggtgacac cactccagcc gccgtggccg aggtcattcg ccggctgtgg gccgggccgg
    13741 agcgactgtt catcatcagt tccgacctga gccattatct gccgtatgag caggctgtgg
    13801 aaacagaccg ccagacgacg gcggccattg aaaacctgga tgccgaaggc atagaggagg
    13861 agcaggcctg cggacggatc ggcatctgcg gcatgcttat ggtggcccgt gaaaagaatc
    13921 tgaaggttga aacagtggat ctgcggaact ccggcgacac ggccggcccg cgcgaccagg
    13981 tggtgggata tggatcgtat gtcttcacat gattcaggcc ccgggctgac tcctcatgaa
    14041 cagcagctgc tgctgtccat cgcccgatgc tccatcgagt atggactcct gcagaaacgt
    14101 cccctgctcc ccgctgaaag cgacatgccg ccggcgctga cgcgcattca ggccactttt
    14161 gtcacgctga ccctgaaaga aaatctgcgc ggctgcatcg gcatgctgga agctgtttat
    14221 ccgctgtgcc gggatgttgc gcagaatgcg tttgcggccg cattccgtga tccgcggttt
    14281 cccccgcttt cggaaaaaga gcttcccgtg gtgaagatgg aaatttcgct tttgacaccg
    14341 cccgtgccgt tccccgtgag ttcagaacag gatctgctgt cgcgcatgcg gccgggggtg
    14401 gacgggctga tcctggaaga cggattccgg cggggcacct tcctgccggc ggtgtgggaa
    14461 gatctgccgg atccgcggga tttcctgatg cacctgaaac tgaaggcggg cctgcccccg
    14521 gattactggt cagacacgct cagggtttcg cgctatacag cgcacaaaat atccgagtga
    14581 tgcgctatct cggccatgaa caaaggtcgt cttccgtgtc tgaaatcccg tccggcccgc
    14641 aggaccagac caccatcccg tagcccggga gagttccgta aatgctgttt tcaatctggc
    14701 agctgttgtc cagatccgta tcgagcacga tttgatacgg ttgtccccac ggatccagca
    14761 gactgccggc atcattcaat ccggaaacgc cgggcgcggc aggccgcacc tccagaaaca
    14821 caatttttcc gggattcacg ctgtggccgg cattgccgtc cccggacacc gcccgcagga
    14881 tattcatcac ataggcattg gggaaatcgc ggccatagcg gaaatctccg tactgtccgc
    14941 tgtgaggagt gggccagatg ccgtattcgc ggaagaagcc ttcacccgcg cggatcagga
    15001 cgcggagatc gccatagacc atctgctgcc gcgcataaag ctgccagccc cagacggcag
    15061 tcacggtcac ggtgcccgcg atcaggagca ggcccagaat gatcgccagc acaggccccg
    15121 ccctgcccgg tttccgggcg gcggggggct ggtttgctcg cagcatgatg cgcttctagc
    15181 tgagtttgcc gatgatcgaa atcaggggca tgaacagagc gatcacgatg gtgccgacca
    15241 caacggccag aaagatgatc aggatcggct cgatgatcga agtcagaccg gccacggtgt
    15301 catccacctc gccgtcatag gtgtcggcga tgcgcatcag catttccggc aggccgccgg
    15361 tttcctcgcc cacttccacc atgctgatca ccatgggcgg gaaaatgccg ctggcctcaa
    15421 tgggcggggc catgttttcg ccctctttca cactgtcgtg gatctggtta accgcccgcg
    15481 caatgacttc gttcccggcc gtgtcgcgca caatgttcag agcctgcaga acgggcacgc
    15541 cggaggtcat cagcgttccc agtgtgcggg aaaaccgggc aatggcggtc tttttgacca
    15601 gtgtgccgaa caccggcgcc gcgatcttgg tgcgatccag gctgtagcgt ccccaggctg
    15661 ttttggcggc aatccgcacc agcacaatca gaatcaccac gcccaccacc acccatgcaa
    15721 tgtgatgcgt aaaggcccgg ctcacattga gcacaaactg cgtgagcggg ggcaggcctt
    15781 cgccttccag cagttcctgg aatatctgct caaacttcgg gatgatgaat atcatcagga
    15841 aggccagaat gccgcccgcc atgcagagca caacgacggg ataaatcatg gcactgatga
    15901 ccttgctctt gatggcctgc gctttttcca tgaattcggc caggcgcacc agcacctggt
    15961 caagcacgcc gccgagttcg ccggccttgg ccatgttcac atagagccgg ttgtaaatct
    16021 tcgggtgctg gcccagcgcc tcggcaaagg tgctgccgga ctgaatggcg tcggctatct
    16081 ccgagagcgc cttcttcagc gcgggattct tctcctggcg ctgcatcacc tgcaggccgc
    16141 gcagcagcgg aagccccgca ttgacaagag tggccagctg ccgggtggag accatgagct
    16201 gtttgggttt cacgcggatc aggaacgaag gcatcttcag ctccatcttc aggcccttgc
    16261 cggaggcggc tttcccgccg ccctttttgg gcatggcccg gcccacttcc gagacctgag
    16321 aggggaacag gtttttctcg cgaatcttgc tcagtgcgga ggtttcgttt tccgcatcaa
    16381 tgacgccctc cacttcctgt ccccttgaat ccagggctgt gtatttgaaa cgtgccattt
    16441 cgcattctcc cgataatgat tatgtgtact tcaaaacctc ttcgaccgtt gtcgagccgt
    16501 ccagtatgca gcggatgccg tcttccctca gcgtcctcat gcccagttcg atggccttgt
    16561 cgcggatgac aattgtgggc ttgcgctgat tgatcagccc ccggaccgga tcgctgaccc
    16621 gcagatactc ataaattccg cgccggcccc gatagccggt ctgactgcag tgcgagcagc
    16681 cttcgccaaa ataaaacgga gtgtcgcgga tgatttccgg cttcaggccg agctgatcca
    16741 gaatatccgc atccggctgg taaggcgtct tgcaggtgga gcagatggtt cgcaccaggc
    16801 gctgccccag aacggcctcc agcgtagacg agatcaggaa gggttcgacg cccatgtcaa
    16861 tgaggcgcgt tacagcgccg gccgcatcgt tggtatgcag tgtgctgaac acaaggtgac
    16921 cggtcagcga agcctgaatg gcgatctccg ccgtctccat gtcgcggatt tcaccgacca
    16981 tgataatgtc cggatcctgg cgcaggaaag cgcgcagcac ccgcacgaag gtcaccccga
    17041 tggcctcgtc cacaggcacc tgaatgaggc cttccacatc atattccacc ggctcctccg
    17101 cggtcatcag cttcgaatcc acgatgttca ggcggcgcag cgcggaatac agcgtggttg
    17161 tttttcctga cccggtgggg ccggtcacga tgatgatgcc gttcggcttt tttatgtctg
    17221 cggagaagtc attgtagatg tcctccggca tcccgatatt ctcgaggtcg agcgagacca
    17281 cggagcggtc gagcacgcgc agcacaacgc tttcgccgaa ctgggtcgga agggtggaca
    17341 cgcggaagtc aatggcgcgg ccgccgatgt tgagctggat gcggccgtcc tgcgggagcc
    17401 ggcgttccgc gatgttgagc cccgagagca ccttcaggcg ggaggtgacg ggaagggcga
    17461 gatgtttcgg gggcggagcc atttcgtaaa gcgccccgtc cacccggtaa cggattttga
    17521 actcggtttc gaacggttcg aagtgaatgt ccgaagcccg gtcgcgaaca gcctgataaa
    17581 gcaccagatt cacaaaccgg accaccggcg tcagattggc cgcgtgctcg agctcctcaa
    17641 ccttgccctc ctgctcgcca aactcgacga gcgtttcggt gccctccagt tcggactcca
    17701 gcgcggaaag catgtccgtc actgaatcgc tctcatcgcc gtagaacttc gcgagatagg
    17761 agttgatatc ctcctcgcgg gcgacgacaa aggaaatatc ccgcgtcagc acaaaagtga
    17821 gctcatccac cacttcggga ctgagaagct cggaagtggc caggaccacg gattgttcgt
    17881 ccgcttccac cggaatcacg ttgtacatcc gggccacgct ggcgggaatg gacttcacca
    17941 cgcgctgcgg gatgtcggtg gccatgaggt tgatcacctc gcagccgagc aggctggcca
    18001 catgctccag gatttcatcc cggctcagga tgctcatgtc cacgatgatg tcgcgcacca
    18061 ttttccctgt gcgctcgtgc tcatccacga catccgcggc ctgggtctgc gtgagaacgc
    18121 cctcctccac gaggtgctgc agcagaagat ctttatattg cagatcggac attgccccac
    18181 cctatttctt tttcttcccg ccggccgcct cgatcttctc catcagggct tccgggtcct
    18241 gtgatttcgt aatcaggtct tcataactga tggagccctc tctgtagagg ctctccagat
    18301 gcgcgtcgag cgtcaccatt ccccacctgg ccccggtttg aatatccgac gtgatacgga
    18361 acgtcttgtt gtcgcgaatc agcgagcgga tggacggggt ggcaatcatg atttcgaagg
    18421 cggctatccg gcccggggca tcatgccgca cgagcagcag ctgggaaatc accgcgacga
    18481 tgctcgaggc cagctgcgtg cgtatctgct cctgctggtt tgtggggaag gcgtcgacaa
    18541 tacggtccac cgttcgcgcg gcgccggtcg tatgcagtgt ggcgaacacg aggtgaccgg
    18601 tttcagccgc ggatatggcg gcttccatcg tctccagatc gcgcatttca ccgacgagca
    18661 tcacatcggg gtcctggcgc aggccgcggc gcagggcctc ttcaaacgac ggcacatcca
    18721 ccccgatttc gcgctgggtg atgatgcttc gcttgtggtc gtggtaatac tcgatcgggt
    18781 cttcaatggt gatgatgtga caatcgcgct caatgttgat cacattgatc atgctggcga
    18841 gtgtggtggt cttccccgaa ccggtggggc ccgtcaccag aaccagtccg cggggcttgt
    18901 agagcagctc cttcacctgc ggaggcaggc cgatttcctc cagcgacatc aggttcgtgg
    18961 ggatctgccg cagcaccatg cccagatgcc ccttctgttt cagcacgctc acacggaagc
    19021 gggcggccgt tccgaaaccg aaaccgaagt ccgtgccgcc gccctcgcga atcttctgct
    19081 ggtgatcctc ggaggtgatg ctcttcatga accgttcggt gtcaatgggc ctgagcggct
    19141 cggcatccag cggatgcagc cggccgtgca gacgaaggat cggcggcgcg ccgactgcga
    19201 ggtgaagatc cgacgccccc tcgtccacca ccagctggag caggtcactg attttgatgt
    19261 cactggatgc catcgtcata gatttgaatc tcccatggtt acacggagca cttcctccat
    19321 cgtcgtcgtg ccggaaatca ccttgcgcag gccgtcctcg cgcagggttc tcatcccgag
    19381 ctcgcgcgcg cgctgccgga gttccgcggc gctggtctgt tcataaatca tgtgccggac
    19441 ctcgtcgttg atcacaaaga tttcgaaaat gcccagccgg ccgcgatatc ccgtcagttt
    19501 gcattcgttg cagccgcggc catggaacag ctgcgcctcg cgaagctgcg cggccgccgg
    19561 gcccagcagc cggtattccg cctcagaggg tttatattcc tccttgcact tgggacagat
    19621 tttacgcacc aggcgctggg ccatgattgc gcgcgtggac gatgccacca ggaagggctt
    19681 gaccccgatg tcaatcagac gggtgatcgc gctcggcgcg tcgttggtgt gcagggtgct
    19741 gaacaccaga tggccggtga gggacgcatt caccgcaatt tccgcggttt ctatgtcgcg
    19801 aatttcaccg atcatgatga tgttgggcgc ctggcgcaga atggatcgca gggctgatgc
    19861 gaaggtcaga ccgatgtcgc ttcggacatg gacctgattg atgcccgaca gctgatattc
    19921 caccgggtct tccaccgtga tgattttccg gtccgggcgg ttgatctcgt tcaaacagcc
    19981 gtaaagcgtg gtggtttttc cggaccccgt cggcccggtc accagcagga taccgtccgg
    20041 gagaccgatc aggcgttcaa agaccttctg gtcgtccgcc aggaagccca gttccggcag
    20101 gccgagcatg agcccctgtt tgtccagaat acgcatcacg atgctttcgc cgtgattggt
    20161 tggaatggag gacacgcgca gatcgaggtc ccggcccatc acattcacct gaatacggcc
    20221 gtcctgcggg acacgcttct cggctatttt catgttggcc atgatcttca cgcggctgat
    20281 gatggccgac tgaaggcgtt tgggcggact ctccacctcg tgcagcacgc cgtcaatgcg
    20341 gtagcggact cggaactgcc gggccagggg ttccagatga atatccgatg cgcggcttct
    20401 gaacgcttca agaatgatca ggctcaccag tttgatgatc ggggcatcgg cctcggtggc
    20461 ctctccgtcc tcggccagga tttccgcccc ggtgggcacg gccacagtgc cctcggtgat
    20521 ttcctcaagc atcgtctcaa ccgtttccgg tgtatgcgca tagtaatgat tgagcgccac
    20581 ctcgatttcc tcggcgggcg ccaccacgcc ttcgacattg catttcagca gataacgaag
    20641 gctgtcgagc gtttcaacat ccagcggatc actgagcgcc acggtgagcg tgttctcatt
    20701 cttgtatacc ggaacaattt tgtaacggcg ggcgattttg ttgggaacaa aatcaatgac
    20761 ttcctgggtg acctccatat tggcgagact gacggtctcc atgccgaact gataggccag
    20821 tgttttcagg atatccagct tgctggccat ctccttttcc accagcacgt cctgcacatc
    20881 cttgtcttcc tcccgtgccg ccgtcagtgc ctcttccgcc tgcgctctcg ttatcagtcc
    20941 gacgctctcg agaatctcaa caatgtattc gtcatttgcc gtcacttggg gcagctccag
    21001 ttgaatgtaa tgattctatt tttcaagaag gcctcatcgt acacagcccc ctgctctttg
    21061 tcaacgaatg gcgccgggcg acttgccggg ccgccggccc gcacagaggc gcagcatgag
    21121 aagcgaaatg gacaggcaaa gcccgggcat gctccacgcg ggcaggccgc ggctcaggaa
    21181 agcgccgcaa cggcatcggc acgggtctca aaaatctcca caaccgcatc cagccggagc
    21241 agttcgatgt tcttgagaat cacagggcgc gggctgcata caacaaaccg gcctcccccg
    21301 agcgccgcct gtcggctgaa ctcaatcatc aggcccatcc cgcggctgtt gatgtaattc
    21361 atgccggaga gatcaagaac cgtcttattg ctggtgcccc ggcacagcgc gccaaccttc
    21421 tcgctgaaaa tgtcaatatt ggacacatcc accggaccgt tcacacaggc gattttcaca
    21481 tcgccctgct cctcaatcag aatgtcaaac aaatgagaat ccattcatac ctccttcaac
    21541 aaagtcaggc ttgcgccagc atgaccccgc tggtctttaa atactgcttc aaatccggaa
    21601 ttgaaatgat gccgaaatga aaaaccgagg ccgccagcag gatggttgcg ccggcttccg
    21661 ccgcctccag gaaatgcttc atttcacccg cgccgccgga ggcgacaacc tccgctttca
    21721 cggcatttct gatggcgcgg atgacgggca aatcataacc cgtgcgggcg ccgtcgccgg
    21781 ccttgctggt cgggaggatg accttcactc catagccgtc cacttccttc gcccactcga
    21841 ctgcatcttt gcccgtggcg gtgcggccgc cgtctatata aacctcgtag cccgacggca
    21901 gcttcggatt ctgatccaca tcaatcgcca cggtgacctt gtccgcgccg aactctttga
    21961 tcatctccct gatgacctgc ggcttgcgga acgccgcgct tgaggtggat attttgtttg
    22021 cgccggcctt cagcacatcg ccggcggatt taacgtcgga aattccgccg ccgacggtga
    22081 aagggatcgt ggtgacatcc gccacgcggc ggacgacatc aagaagcgtc ccgcggtttt
    22141 caatggtggc cgtgatgtcc agcatcgcga gttcatcggc gccggccgcg cagtaggcct
    22201 ttgcgcatgc gacgggatcg ccggcatcct taatgtccac aaaatgcacg cccttgacca
    22261 cgcgcccgtt ctgcatgtcg aggcacggca tgattctgac cggctgcttc atattgaata
    22321 ttcctttctt caaactgctc acagcatctt tgtattggaa ccatcccgca gtttccatct
    22381 gattctgtaa atttttgcat ccgcagctta tggtgccgac aagcaggaat gccggaaaac
    22441 acggatatag tactatttta gtactatttc catgtttttg cagtttttgg tcattttttg
    22501 acgttttggg gctattttcc gctgtttttg gccgtttttc ggtgtttttg ggccattttt
    22561 tgcgtttttt gagcattttg gggccttttc ttgatgtttt tcggatatcc gccacaaaaa
    22621 gcacataatt cacaaaatcg aatcgggaac tcaggaagac aggaaatatt tcgacaggat
    22681 caacaggatt tacgggattg cagggtaaca atgatccgtt tcatggtttc attccatcgg
    22741 ccatttgggg atggcttggt ctatttcccg catgagttcg atggtgcggc cgagggcggc
    22801 tacaattttt ttgtagtgct ggagatcgtc gttggtcaat acgcgctcgc ggcggtcttt
    22861 gagccatttt tcgcagacct ggtagccgcc gatggtgaag ttccagacat tggaaggcac
    22921 gttgtcgaaa tattgcgttt tgttgatata cacgcggcca tccttgaatt tcgggaagcc
    22981 tttcgcgacg atgttgtccc cggcaacagg gaaggacgtg gcgggcttgt tcaattcggg
    23041 cgattccatc aggtgcagcg cggtgagttc gccgccgagt ttgaccagcg cggcaaagag
    23101 ctttttgtcg gaggtgagcg gtacgcgcgg gaaatcaatt ttcagaaagt cggcgtagcg
    23161 cgtgcggtag gtcggcgaat gaagcaccgc atagatgtaa taaaacacat cctccggccc
    23221 gacggttttc ttcaaatccc cctgcccgtc cg
//
LOCUS       DAOQWR010000024        22264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2690_length_22264_cov_5.212211, whole genome shotgun sequence.
ACCESSION   DAOQWR010000024 DAOQWR010000000
VERSION     DAOQWR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22264
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_22264_cov_5.212211"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKK36_03410"
     CDS             complement(<1..838)
                     /locus_tag="PKK36_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR93640.1"
                     /translation="MFKRILITGATGFLGHHVLPVMRKEFDAEIIAVGSRNYDLLKPG
                     EPDRMMQEIKPDCVLHMAAKSGGIIDNKARPADYFYQNLFMNLHTFEAAFRHGAKKFM
                     TFMGGCSYPATATSPIGEDQMWKGLPQIESAGYSVAKLMNLTQSWAYRVQHGFNSVVL
                     IPGNMYGEWDNFNLTQAHVIPSLIRKYLEAEERGDPEIIAYGTGKPTRDFVYAGDVAA
                     TVPWFLKNYNSSDPVNISAGKRISIRELAETIKKVTGFRGKIVWDTSKPDGQMDKIFD
                     VAR"
     gene            complement(844..1854)
                     /locus_tag="PKK36_03415"
     CDS             complement(844..1854)
                     /locus_tag="PKK36_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNR93641.1"
                     /translation="MTARRHLHKAMRPLLGLLILYIVFRRIPVDELASVLLHSLHAWP
                     WWISGIAVTFLGLLAGALRWHRILRDQGFTLPFSESFRYFFVGQFFNSFFPGACGGDV
                     VRAYSVIRSKGTNKTGAVMTVLMDRAIGLLVFVILGCIMIAFRYRLFVYSRGTRLTAY
                     LMGAFLLATSAGLIVLFRRNLFEHWPLFKKFEETTRAGALLRKAYDVLLVYRSRPRLL
                     VSAIGLSFVNLAALTSACFFFGHSLGIGISWFDYFTFFPIITVFTAVPITPGALGIRE
                     ALFAGMLHTVDVSSSQAVPLSLLMYAGGLVWSLFGGLIFLLQPNRPHALPIDTAATDR
                     YC"
     gene            complement(1851..2765)
                     /locus_tag="PKK36_03420"
     CDS             complement(1851..2765)
                     /locus_tag="PKK36_03420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR93642.1"
                     /translation="MRVLLTGAAGFVGRHLAAELTHTGHEPVLSDVRTGIPGIRAADL
                     RDPDALCSLVRDTRPDACVHLGGIAFVPVGWDDPELVMDVNLIGTIHLLEAFRLHAPA
                     ARLLIVSSSEVYGRRPVETPLTEEAPFAPSNPYAVAKAAADLTALLYAKRYGMPVMTA
                     RPDNHTGPGQSPQFVTTAFALQLLDIRRGTSAGSIKVGNLNSVRNFTDVRDVARAYRL
                     ILEKGYAGCAYNIASGNMAEIGSILDMLCDIAGIQPQITVDQERYRPADALPALDTAR
                     LRNHTGWTPQIALQVTLSDIFNHLQAGG"
     gene            complement(2769..3734)
                     /gene="gmd"
                     /locus_tag="PKK36_03425"
     CDS             complement(2769..3734)
                     /gene="gmd"
                     /locus_tag="PKK36_03425"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR93643.1"
                     /translation="MKALITGVTGQDGSYLAELLLSKGYEVYGMCRRASTETYERIAH
                     IKDRIELVQADLLDQLSLIKLFEHIMPDEIYNLAAMSFVPTSWSQPVLTGEFTAIGVT
                     RVLEAMRQVCPQAKMYQASSSEMFGKVREVPQTEMTPFHPRSPYGVAKVYGHYITVNY
                     RESYDLFACSGILFNHESPRRGKEFVTRKITDGVAQIKLGVAKELRLGNLDAERDWGF
                     AGDYVEAMWLMLQQEQPDDYVIATGQTHSVKDFCRKAFARAGLNYEDYVVLDSRFVRP
                     AEVDQLLGDPSKARKVLGWKQKTSFDELVNMMVDADLERWAAVKG"
     gene            complement(3766..4680)
                     /locus_tag="PKK36_03430"
     CDS             complement(3766..4680)
                     /locus_tag="PKK36_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNR93644.1"
                     /translation="MLFALTSRWNAGRHAAGEEMIEEILDLGFPAVELGYNLRMDLVP
                     GVQSMVRQKAVIVTSVHNFCPVPVGAPAGHPELFTLADPDPRVRQLAVQHTTRTIRFA
                     AETGARAVVLHAGNVDMKRLTERLLALYEHGQQFSPKFEKIKLKMLTLREKKVRRQLE
                     YLYSGIEALLPVLQECGIRLGIENLPNWETLPTETEIEALLKHFNSPLLGCWHDTGHA
                     AIRRFFGLINPERWMERLEEHLIGLHIHDARAPAMDHLMPPSGEIDFTQYKRYAARDV
                     CRVFEPGPSATASEIKAGLDYIRTLWSD"
     gene            complement(4748..4927)
                     /gene="rpsU"
                     /locus_tag="PKK36_03435"
     CDS             complement(4748..4927)
                     /gene="rpsU"
                     /locus_tag="PKK36_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018923713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNR93645.1"
                     /translation="MAEVKVKKGETVEKALRRLKKKLDKEGTMREIRSHRHYEKPSER
                     KRRKAARARTRINRY"
     gene            complement(5021..6325)
                     /locus_tag="PKK36_03440"
     CDS             complement(5021..6325)
                     /locus_tag="PKK36_03440"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNR93646.1"
                     /translation="MKITVVGTGYVGLVTGACFAEVGHDVMCVDNDESKIAMLRRMEM
                     PIYEIGLQELVERNVAGKRLHFTTSIEEGTDFAEVIFIAVGTPPGYRGEANLSYVEQV
                     GRTVAEHMTSYRLLVEKSTVPVNTGMQLKRTITKYIEGGLDFDVASNPEFLREGTAIE
                     DAFQPDRIVIGVESERAAKLLREVYDPIVKKSGCEFLVMNIASAELTKHASNSFLAMK
                     ISYINAVSRVCELAGADVEQVAHGMGLDSRIGQKFLNAGVGYGGSCFPKDVDAFIHLS
                     DQLGYDFQLLKEVQRINQNQREHMLQKIKHELWVMQDKTIAILGLAFKPGTDDLRQAP
                     SLYFVPQLIEARAKLKLWDPIASDNFKKLYPDLTYCDDILDCAKDADMVLILTEWPEV
                     RDLDLKKLKKAMAVPVVMDGRNVFDPAEMHRLGFIYHSMGRP"
     gene            6457..7332
                     /locus_tag="PKK36_03445"
     CDS             6457..7332
                     /locus_tag="PKK36_03445"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR93647.1"
                     /translation="MSRTCMVTGASGYLGGLVLSRMASRGPAVGTAWRPSAPGLVSCD
                     LTDPLAVKDLLVQHEPDTVVHCAAWRDPDRCEEFPDEAALINSASARFICAALPPEAV
                     FVLISSDYVFDGTSPPYTEDDSVCPVNVYGRTKVTAEQAALARGNSLVIRPPVLIGQE
                     LTGQGGFIAQMVSALRSERPADVDNVLVRFPVFGPDVAEAVEFLLAKRAAGVFHVSGE
                     QGGTRWQWTLRTAALLGLQTDHLRPSRVIVPRRAARPPDSHLSTGKIKGLGFSGFTGF
                     DEVVRRAVPFLSPSS"
     gene            complement(7316..9385)
                     /locus_tag="PKK36_03450"
     CDS             complement(7316..9385)
                     /locus_tag="PKK36_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNR93648.1"
                     /translation="MGEVQLRIDTEESPSIPMPAQRPAIPPPPQLKAPAHRHLRFAHK
                     ITLLAVCMIILTGLSITFLTVTQFKREMYERERSSAYMAYSAVVNYLTAHHHSHRGTF
                     SAKAMDYVIRNKMLRLREDMDAYITHRPERLVVFDERGAPIYEFIRTEEFGPPRAAPL
                     PADRLPQKVRLDYDRRSGMVHLRGPIAVQDEDVPGYVEISFPCSIWQKVNILFTQAFL
                     IMCGVILIAIVVTLTLDQRVLAPIAALTAAAQRIRKGDLSQQVPVKSDDEIGTLASTF
                     NNMISSLKMRISLLHSIQDWTVRIGKELESERLYGTLVAMFSVVSTSRACRIYTYDKA
                     RKELQIQLERGAAHLPEPQNDQLTIRALHQRWTEFLKADGKIVSDPTGAVELAIPLIS
                     GEKPIGAIRIGRKQDGTVFSDEEVTILQTLAQHASVSLDNAHLYEELSEKERIEQEMI
                     WARRIQQELLPQCAPDIAGYDIFGGSIPALEVAGDYFDYISIDGNRWYLVIADVSGKG
                     VPAALIMSITRSLLQAYVDYESSPLDVLNKVNRRISQTIESEMFVTIAVACLDVRENR
                     ISIARAGHEPVFMFSGKDQIERIAPAGAAMGLLDPVAFRQSLRPFEGAMACGDVVLLY
                     TDGITEAQNKDGEEFGYDRIEDLLRKHNAESAENIYNIIINAVEEFAGDQPQHDDITM
                     LIVKKTD"
     gene            9584..11731
                     /gene="glgP"
                     /locus_tag="PKK36_03455"
     CDS             9584..11731
                     /gene="glgP"
                     /locus_tag="PKK36_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNR93649.1"
                     /translation="MAIEPIPVADIQVPQEFARLRDLAYNLWWSWTPEARLLFSNIDA
                     YLWGRYRNPVELLINIEPHHWDRLLNGGGNFMSQYRKVIRDFDRYMNPDEPTWFQQQY
                     PEYKSDRIAYFSTEFGLHESLGIYCGGLGVLSGDHAKAASDLGIPFVGVGVLYRKGYF
                     HQAIDLDGRQHHIYNNFDFYRLPLRPMHNAAGRDLVVTVDYPDRQVYARVWQLAVGRV
                     QLYLLDTDILENDPGDRPMTSQLYVRGREMRLCQEIMLGIGGVRALRELKIQPAVWHM
                     NEGHSAFQVVERMRAHLAEGMTWKEASEAIRVNTLFTTHTPVPAGNEAFEASLVEKYM
                     RVFTDKMGVDVHQMLDLGKSYPEGGSQPFNLTAVGLRFSHYSNGVSKLHGEVANHMWH
                     HLYPGAKPTDQNIKSVTNGVHITTWLGHDIRTIIEEALGTGWIGHEDDPEFWKGILKI
                     RDKDLWAAHQSQKDRMIRLVRHRLVEQFARHGKGPDSIREMNRMLDPNVLTIGFARRF
                     ATYKRAGLIFRDYDRLRQIITNAEHPVQIVFSGKAHPADEPGQDLIRDIVTKAIYSDL
                     KGYIFFVEDYDIRIARHLVQGVDVWMNTPRRPREASGTSGMKAAINGALNFSVSDGWW
                     PEAHNGKNGWIIGSEHHIDNEDEQDYRDSTSLYDILQNQIIPLYYQNRTAGCPCEWVQ
                     WMKESMATIIPGFSAARMLQDYTRDAYMPTAQR"
     gene            11738..12742
                     /locus_tag="PKK36_03460"
     CDS             11738..12742
                     /locus_tag="PKK36_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR93650.1"
                     /translation="MDLLVCRPGFEDALRDEIAQRLGGKAVCLCPVPGMVEVRASVPL
                     PPEFIFERRRMPDAGFIPDQLLKPVTAEQVRQMLGPALQGNLPWSICSFPAGSGTVGN
                     LGPRVKGIEKAVLRVAREHFPETGRQMTDTQAGLLLQLCLTPRGLYHSCAAPCLQAGS
                     PLRMHQDSRAPSRSYLKLEEAFQLMNRQPRKNETAVDLGAAPGGWTYALIRRGCHVTA
                     VDHGPMKLPPHEPGWGRVRHIRANGITFEPPRDLCPVDWLVSDMLVAPGVVLGLLRRW
                     IGARRMRFFVCNIKIPQQNPCAAVKPLEDFLSAQRKVEFRLKQLYHDRREITIMGQLK
                     "
     gene            12739..13839
                     /locus_tag="PKK36_03465"
     CDS             12739..13839
                     /locus_tag="PKK36_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNR93651.1"
                     /translation="MIFRTCGKTGKALSAIGAGTMRFKTPANDDDVRDAARVLLHAHE
                     KGINYFDTAPYYCGDRSEDITARAIRDMKPGSFYLSTKSGKPAGTELRAQLETSLKRL
                     GVDCIHFFHVWCVMDPADWVQRKEKGAVAAALQAQKEGLVEHVVFSTHMTGTETAAVI
                     RENIFEGMTIGYNALNFPYRQEGIEAAEKASIGVVCMNPLAGGLIPAHAKRFNFIRRA
                     DDGNVVTAALRFLLSHKAITSALVGFANKSEVDAAVSAIEQFKPYSPEEINQLKRRIE
                     DSFDQLCTGCQYCLPCPAGVPIPKYMDSYNMKMLSGTDAAVLDRLKWHWDIQAADAGA
                     CTACGLCESRCTQHLPIIERLRGISRIPEPGN"
     gene            complement(13913..14431)
                     /locus_tag="PKK36_03470"
     CDS             complement(13913..14431)
                     /locus_tag="PKK36_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR93652.1"
                     /translation="MKNKLVPWKRKNELQRLDEGFGLNPFSQLRNQIDELFEEAFDSF
                     PSFGRWPALGRLGSDMKMVSPDFEVTSTEDEIQVKAELPGMTENDIEVTVDENSVTIG
                     GNKKEEREEKKRDCYFSEVRYGHFSRTIPLPAGIDREKAKAQFKRGVLKLTLPRTEKA
                     KTERKQIKIESE"
     gene            complement(14452..14850)
                     /locus_tag="PKK36_03475"
     CDS             complement(14452..14850)
                     /locus_tag="PKK36_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR93653.1"
                     /translation="MTQETRNLAKKESQSVELPQDRPVFVPATDIYETDKAIFLRCSM
                     PGVSSDSLDVVLEDSVLTLTGHQSWKTPDDFQLVYQEYETGVFRRSFRISEEIDQGGI
                     KAVMKNGVLNLELPKSPKAQPKKIKVEMIS"
     gene            complement(14865..15278)
                     /locus_tag="PKK36_03480"
     CDS             complement(14865..15278)
                     /locus_tag="PKK36_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR93654.1"
                     /translation="MTGWEPFSGLRALQREMDSLLDGYSHRSRSDFAVNVWSNGEKAV
                     VSAELPGCDAADIEISTLKDFLTITGKVKQEETIKDAVCHRAERSCGEFSRTIRLPFE
                     VEQDKAKASYSQGVLTVELARAEASKPQKIKIESA"
     gene            complement(15748..15822)
                     /locus_tag="PKK36_03485"
     tRNA            complement(15748..15822)
                     /locus_tag="PKK36_03485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15788..15790),aa:Gly,seq:ccc)
     gene            16306..21792
                     /locus_tag="PKK36_03490"
     CDS             16306..21792
                     /locus_tag="PKK36_03490"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HNR93655.1"
                     /translation="MALLLFALMASPARAQMLESFEYGVPPPGWTKTNLLGGSGWYRL
                     PVGVMPLPGWGNGTSSVPPTANAGSANAYCSWTTGGGASDGYHSDQWLISPQLNGLTA
                     TSSLSYWLRFAFTNFPDTVYVRISTAGPAPANFTRVVYTNIWSRGGNPSGQFGPWTRY
                     DVNLGALGIAAGTPIWVAFQEYEWDNIHNGAAVQLDVISSDLTAPPQPRISPTSLTFT
                     AYCEGSDPAPQTFSIQSIGSSGMSYSRTFAFGAGPTNWLTPGGLLSGTVAFQNSQVFT
                     ASVSAAGLDLGTYCATNIITVPGATNSPLRIPITFHVIRRPQTLSFPNPGAQWTTNRV
                     GLAGTSSSGLPVTFSIYSGPGVVTGATNLTFTGTGTVRVIAWQLGNFYYDAAPCVTNS
                     ITVSKPHAVISFTNMSHVYDGSPHGATVITDPEGLTLVTTYNGSSSLPVAIGSYAVTS
                     TVNDAMFDGTAVSTFTITRMVQSVTFPDLGPQWTTSRVGLAATASSGLPVGFSIASGP
                     GSISGGTNLSFTGAGAVSIDALQVGDADWLPASATRTIEVAKTETSFIFNDLSQIYNG
                     NARVVTATTVPPGLNVEITYNGAAPAPIAAGSYTVTGVINDVIYSGMSVATLTVGRAD
                     QVITFPDLSPRTTNEAVGLSASASSGLPLTDFAVMAGPGMVNGTNLTFSGAGDVLVAA
                     LQAGDENWNPAAAVTNLVKAFSLVPNNGPASGGNTVLITNGRIGDGAGITNATLCGIA
                     ATILDQGTNWVSVQCGPGGAGTGSILLQSASDIVVSNAYTYNPAGTIFAVAPAVGSYV
                     GGYPVVITGTNLCSGDVTNVTLCGASAAVSSQSPTQIIVTAGAGALGLGDVRVFSVSF
                     GETVLSNAFTYAVPEMTVLGAGGEVVGSGDAAALSAGTDFGSQLVFDAATNWFEITNS
                     GAASLYISSVTTSGAGAAFFAACNMPAGIQPGGAAGFSIVYAPTTVGVHSAALWLVND
                     STTTPYRINLAGASLKKDQHDLTFTPATPQTFNTTNGLAAGGGQSSGGWTYTVESGAA
                     EIAGETNLWMRTGTGSVTVRATRAGDANWNEAWIEAVVNAARASQTINFPNPGTQSAT
                     NIVDLSADSSSGLPVIYGVVSGPAVFTGTNLNQVAFTNLGTVVLSASQAGNADWNPAA
                     TSVTFEVDRVPAQIVFTNLCYVYDGTEKTATVSTIPPGLNVVVTYPPLMMPPVNAGTS
                     RVEAVIEDSLYRGSDTNWLVIACATQGIGFAAIADQAITSTLELAGTASSGLPVSFAA
                     SGPAVLSGGTNLSFMGTGLVSIAASQEGSTNYLPAAVTNSFLVYPLSPLVSGPFHLGA
                     AVTGALMGATIENVYGANAFQRGIVWSPAPGFDPVSASRAEELGDFGAGTWTQQVSGL
                     SAGLTNFYRAYAVNTAGTGYTAEAWVLMRPAAPVAEDASDHNPDSFQANWQLTAGAWT
                     YWVDVSEDAGFASFVPGYGGRPAGIANSIVVTGLTSGVTYHYRIRAENDAGLSDWSNV
                     KGCMTRPLLTIVCQPGSGGTTVPAAGTLTVDAGDPVAIQARTNSGYAFVRWDQIGGGA
                     VASPLASTTTVVLVSNSIVTAVFAQQAAGAAAVIESWQTNRLSGTLLASIRICNNQTN
                     GVRMAEPFHFCVQATAAYRLMHPSGTNVLTKWPYADITAQIKAGAGDGILDPGECVLV
                     TNIEFFVKTLVAPAAFNWKVVAVLLPPAAAMDTDGDGIPDVWEQMFPATLDCLFAPDA
                     GIDSDGDTYDNHDEYTAGTDPEDPASYLRISALTNAFGIGRTIQWPGESGRYYRVWGT
                     TNLLDAYQVIGSGVFATPPVNVFTDAVLDVQNSAFYKIEAGLTP"
BASE COUNT         4477 a         6458 c         6647 g         4682 t
ORIGIN      
        1 gacgggcgac gtcaaatatc ttgtccatct gcccgtccgg cttggaggtg tcccaaacaa
       61 tctttcctcg aaatccggtc actttcttga tggtttcggc cagctcgcgg atagaaatgc
      121 gttttccggc ggagatgttg actggatcgc tgctgttgta attcttcagg aaccagggga
      181 cggtggcggc gacatcgccg gcatagacaa aatcgcgggt gggctttccg gttccgtagg
      241 cgatgatctc gggatcgccc cgctcttcgg cctcaagata tttcctgatc agtgagggaa
      301 tgacatgcgc ctgtgtgaga ttgaaattgt cccattctcc gtacatgttc ccgggaatca
      361 gcaccacgga attgaatccg tgctgcacgc gataggccca tgactgggtg agattcatca
      421 acttggcgac agaatagcct gcgctttcga tttgcgggag gcctttccac atctggtcct
      481 cgccgatggg ggatgtggcc gtggccgggt aagagcatcc tcccatgaag gtcatgaatt
      541 tctttgcgcc gtgccgaaaa gccgcctcga aagtatgcag attcatgaag agattctgat
      601 agaaatagtc cgccggccgc gccttgttgt cgatgatgcc ccctgatttt gcggccatat
      661 gcagcacgca gtccggcttg atctcctgca tcatcctgtc gggttcgccc ggcttgagaa
      721 ggtcgtagtt cctgctcccg accgctatga tctcggcgtc aaattctttc cgcatcacgg
      781 gcagaacatg gtggcccaga aagcccgttg ccccggttat aagaattctt ttaaacatat
      841 ctgttaacag tatctgtcag ttgctgcggt gtcaatgggc agcgcatgcg gccggttcgg
      901 ctgcaggagg aatatgagac cgccaaacag gctccacaca agccctcccg cgtacatcag
      961 cagggagagc ggcaccgcct gagaggagct cacatccacg gtatgaagca tgccggcgaa
     1021 aagagcttca cggatgccca gtgctcctgg agtgatcggg acggccgtga atacggtaat
     1081 tatcgggaag aaggtgaagt agtcgaacca ggatatcccg atgcccagac tgtgtccgaa
     1141 aaagaagcag gcgctggtca gggcggcgag gttgacaaag gacagcccga tcgccgagac
     1201 cagcagcctg gggcggctcc ggtagacgag caggacatca tacgctttgc gaagcagggc
     1261 tccggcacgc gtggtttctt cgaacttctt gaataaaggc cagtgttcaa acagattcct
     1321 tcggaagagt acgatcagcc cggccgacgt cgccaggagg aatgctccca tgagatatgc
     1381 cgtcaggcga gtgcctcggc tgtatacaaa cagccggtag cgaaaggcga tcatgatgca
     1441 cccaagaatg acaaacacca gcagcccgat cgcgcggtcc atcaggaccg tcatgaccgc
     1501 accggttttg tttgtccctt tgctgcgaat cacggaatac gcgcgcacga catcccctcc
     1561 gcatgcgccc gggaagaatg aattgaagaa ctggccgacg aagaagtatc tgaagctttc
     1621 cgaaaacggc agggtgaatc cctgatcgcg taaaatgcgg tgccagcgaa gcgctcctgc
     1681 gagcagcccg aggaaggtga ctgcgatgcc ggatatccac cagggccagg catgcaggct
     1741 gtggagaagg actgatgcca gttcatccac gggaatgcgc cggaatacaa tatagagaat
     1801 cagcagcccc agcagcgggc gcatggcctt gtgcagatgt ctgcgggctg tcatccgccg
     1861 gcctgaagat gattgaaaat atcagacaga gtgacctgaa gcgcgatttg cggcgtccag
     1921 ccggtatggt tgcgcagccg ggccgtatcc agtgcgggca gggcatcggc cggcctgtag
     1981 cgttcctgat ccacggtgat ctgcggttga atcccggcaa tatcgcacag catgtccaaa
     2041 atagagccaa tctccgccat gttcccggac gcaatgttgt aggcgcagcc cgcatagccc
     2101 ttttcgagaa tgaggcgata ggcgcgtgcg acatcgcgga catccgtgaa attgcggaca
     2161 ctgttgagat tgccgacttt gatggaaccc gccgatgtgc cccgcctgat gtcgagcagc
     2221 tggagcgcga aggctgtggt gacgaactgc ggggattgtc cggggccggt gtgattgtcg
     2281 ggtcgcgcgg tcatgacggg catgccatag cgctttgcgt aaagaagtgc ggtcaggtcg
     2341 gccgcggctt tggctacggc gtagggattg gagggggcaa acggggcctc ttccgtgagc
     2401 ggagtctcga caggccgtcg cccatagact tccgaagagc tgacaatcag cagtcgggcg
     2461 gccggcgcat gcaggcggaa tgcttccagc agatgaatgg tcccgatgag attcacatcc
     2521 atcacgagtt cgggatcgtc ccatccgaca ggcacaaatg caatgccacc cagatgaaca
     2581 caggcatccg gccgggtgtc gcgcaccaga gagcaaagtg catccggatc gcgcaggtct
     2641 gccgcgcgga tgcccgggat tccggtccgc acgtccgaaa gcacgggttc atgccctgta
     2701 tgggtcagct ctgcggccag atggcggccg acaaagcccg cggctccggt gagcagtacc
     2761 cgcatgactt accccttgac agcggcccag cgctccagat cggcatccac catcatgttg
     2821 accagctcgt cgaaactggt cttctgcttc cagccgagca cttttctggc cttggatgga
     2881 tcgccgagga gctgatccac ttccgccggc cggacaaaac gtgaatccag caccacataa
     2941 tcctcgtaat tgagcccggc gcgggcgaac gccttgcggc agaaatcctt gaccgaatgg
     3001 gtttgcccgg tggcaatcac atagtcatcg ggctgctcct gctggagcat gagccacata
     3061 gcttccacat agtccccggc aaagccccag tcgcgctcgg catccaggtt cccgagccgg
     3121 agttcttttg caacgcccag cttgatctgg gcgacgccgt cggtgatctt gcgggtgaca
     3181 aactccttcc cgcgccgcgg gctctcgtgg ttgaagagga tgccggaaca ggcaaacagg
     3241 tcgtagctct cgcgatagtt tacggtgatg tagtgtccgt acaccttggc cacgccatag
     3301 gggctgcggg ggtgaaaggg ggtcatttcc gtctgcggca cctcgcgtac cttgccgaac
     3361 atctcgctgc tcgaagcctg atacattttc gcctgcgggc aaacctgccg catggcctca
     3421 aggacgcgcg tgaccccaat ggcggtgaat tcccctgtga gcaccggctg gctccacgag
     3481 gtcggcacaa atgacatggc cgcgaggttg tagatctcat ccggcatgat gtgctcaaag
     3541 agcttgatca gcgacagctg atccagcagg tcggcctgca cgagttcaat gcggtccttg
     3601 atatgcgcaa tgcgctcata ggtctctgtg gatgccctcc ggcacatgcc atagacttca
     3661 tagcccttgg atagaagcag ttcggccaga taggagccgt cctgtcccgt aacgccagta
     3721 atcagtgctt tcattcattc acccgttgtt tgttgttgca agctgtcaat cagaccataa
     3781 ggtcctgatg taatcaagac cagccttgat ttccgacgcg gtagcggaag ggcccggctc
     3841 aaacacgcgg cagacatccc gcgcggcata gcgtttatac tgcgtaaagt caatctcgcc
     3901 ggatggcggc atcagatgat ccatggccgg cgcgcgcgcg tcatgaatgt gcagaccgat
     3961 caaatgctcc tccagtcgtt ccatccagcg ttcgggatta atgagcccga agaacctgcg
     4021 aatggctgcg tgcccggtgt cgtgccagca tcccagcagg ggggagttga agtgcttcag
     4081 cagcgcctca atttccgttt ccgtcggaag agtttcccag ttgggaagat tttctattcc
     4141 caggcgaatg ccgcattcct gaaggaccgg cagcagggct tcgatcccgc tgtagagata
     4201 ttccagctgc cggcggactt ttttctcccg gagggtcagc atcttgagtt tgatcttctc
     4261 aaatttcggc gagaactgct ggccgtgctc atagagggcc agcaggcgtt ccgtcagtcg
     4321 cttcatatcc acattgccgg catgcagcac gactgcgcgg gcgcctgttt ccgcggcaaa
     4381 gcggatggtc ctggttgtgt gctgcacggc gagctgacga acacggggat ccggatcggc
     4441 aagcgtgaac agctccggat ggcctgccgg cgcgcccacc ggcacgggac agaaattatg
     4501 cactgacgtg acaatgaccg ccttctgccg gaccatgctc tgcacgcccg ggaccagatc
     4561 catgcggaga ttatagccca gctcgacagc cggaaaaccg agatcaagaa tctcctcgat
     4621 catctcctcg ccggctgcat gtcggccggc gttccagcgg gatgtcaggg caaaaagcat
     4681 cgcgatgctc cgggaagcga agggcggtgg aaagaaaacg gccggccctc agggccggcc
     4741 gtgaatgcta gtaacgattg atgcgggtgc gggcccgggc ggccttgcgg cgcttgcgct
     4801 cgctgggctt ctcatagtgc cggtgcgaac ggatttcacg catggtgcct tccttgtcca
     4861 gcttcttctt cagccggcgc agcgcctttt ctacggtttc accctttttg acttttactt
     4921 cagccacgtg ttaactcact cccttctcaa tccaaatcgt gaagcacaga acctattgaa
     4981 aaccccgcag gatgtcaatt tgaagcgcaa tctttctgta ttagggccgg cccatcgaat
     5041 ggtagatgaa tcccagcctg tgcatttccg ccggatcgaa cacattgcgt ccatccatga
     5101 ccaccggaac cgccatggcc ttcttgagct tcttgaggtc caggtccctg acttcgggcc
     5161 attccgtcag aatcagcacc atgtccgcgt cttttgcgca atccaggatg tcgtcgcagt
     5221 aggtcaggtc cggatacagc ttcttgaaat tgtctgaagc gatggggtcc cacagcttca
     5281 acttcgcccg ggcctcgatg agctgcggca caaaatagag cgacggagcc tggcgcaggt
     5341 catccgtacc cggcttgaag gccagcccca gaatcgcgat ggtcttgtcc tgcatgaccc
     5401 acagctcatg cttgattttc tgcagcatgt gttcgcgctg gttctgattg atgcgctgca
     5461 cctccttcag gagctggaaa tcgtagccca gctggtcgga aagatggatg aaggcatcca
     5521 catccttcgg gaagcaggag ccgccgtagc cgacgcccgc attcaggaac ttctggccga
     5581 tgcgggaatc aagccccatg ccgtgcgcca cctgttcaac atccgccccg gccagctcgc
     5641 acacccggct gacggcgtta atgtaggaaa tcttcatggc caggaacgag ttcgaggcat
     5701 gcttcgtcag ctcggcgctg gcgatgttca tcacgagaaa ctcgcagccc gattttttga
     5761 caatggggtc atagacttcg cgaaggagct tcgccgcgcg ttcgctttcc acgccgatga
     5821 caatgcggtc cggctgaaat gcatcctcaa ttgccgtgcc ctcgcggagg aattcgggat
     5881 tggatgccac atcaaaatcg agcccgccct caatgtactt ggtgatggtg cgcttgagct
     5941 gcatcccggt gttgaccggg accgtgcttt tttcaaccag caggcgatag gatgtcatat
     6001 gctccgccac ggtgcggccg acctgttcca cataggagag gttggcttcg ccgcgatacc
     6061 ccggaggggt tccgacggca atgaagatca cttccgcgaa atcggtgcct tcctcgatgg
     6121 acgtcgtgaa gtgcaggcgt tttcccgcca cattgcgttc caccagttcc tgcagcccga
     6181 tttcataaat cggcatctcc atgcgccgga gcatggcaat tttgctctcg tcattgtcca
     6241 cgcacatgac gtcgtgaccg acttctgcga agcaggctcc tgtgaccagt ccgacatatc
     6301 cggttccaac cactgtaatt ttcatatcct tctcctcttg cgaaaacaaa gccgtgatcc
     6361 tattcatccg ccgcggataa tgcaactgct taacgccggc tctccgcatt ttttgaggct
     6421 tgcctgaggc agggtccccc ggtgtataaa cccggcatga gcaggacctg catggtgacg
     6481 ggcgcgagcg gatatctggg cggtctggtg ctgtcccgaa tggcgtctcg cggccccgcg
     6541 gtcgggaccg cctggaggcc ttccgcgcca gggcttgtct cgtgcgacct gacagacccc
     6601 ctcgctgtga aggatttgct tgttcagcat gagccggata ccgttgtgca ttgcgccgcg
     6661 tggcgtgatc ccgaccgctg cgaggaattt cccgatgagg cggccctgat caactcggct
     6721 tccgcccgct ttatatgcgc cgcgcttcct cctgaagccg tttttgtgct gatcagctcg
     6781 gattatgtat tcgatggaac cagtccgccc tacacggaag atgactcggt gtgtccggtg
     6841 aatgtctacg gccggacaaa ggtgacggcc gaacaggccg ctttggcccg cggcaattcg
     6901 ctcgtgatca ggccgcccgt tctgatcggg caggaattga cgggacaggg gggcttcatt
     6961 gcacagatgg tttcagcgct gcggtcggaa cgcccggcag acgttgataa tgtgctggtg
     7021 cgcttccccg tgtttgggcc tgacgtggcc gaggctgttg agtttctcct tgcgaagagg
     7081 gcggcgggag tctttcacgt cagcggagag cagggaggca cgcgctggca gtggaccctg
     7141 cgaacggccg cgctgctggg cctgcaaaca gatcacctgc gtccgtcccg ggtcatcgtg
     7201 ccccggcgcg ccgcccggcc gccggactcg catctttcca cgggaaaaat caaaggactt
     7261 ggattttccg gatttacggg tttcgacgaa gtggtgcgga gagcggtgcc ttttctcagt
     7321 ccgtcttctt gacgatcagc atggtgatat cgtcgtgctg cggctgatcc ccggcgaatt
     7381 cctcgacggc gttgataata atgttgtaaa tgttttctgc ggactccgcg ttgtgcttgc
     7441 ggagcagatc ttcgatccgg tcgtagccga attcctcgcc gtctttgttc tgggcctcgg
     7501 tgatcccgtc agtatagagc agaaccacgt cgccgcatgc catcgcgcct tcaaaaggcc
     7561 ggagcgactg cctgaaggcc actggatcaa gcagccccat ggcggcaccg gccggcgcga
     7621 tgcgttcgat ctggtccttg ccgctgaaca tgaaaaccgg ttcatgaccc gcccgggcaa
     7681 tggaaatccg gttttcgcgc acatcaaggc aggcgacggc aatggtgaca aacatttccg
     7741 actcaatcgt ctggctgatg cggcggttca ccttgttgag cacgtccagc ggggacgact
     7801 cgtaatccac ataggcctgc agaagtgacc gggtgatact catgatcagc gccgcgggaa
     7861 cgcccttgcc ggacacatcg gcgatcacca gataccatcg gttgccgtca atgctgatat
     7921 agtcaaaata gtccccggcg acctccagcg cgggaatgct tccgccgaag atgtcgtatc
     7981 cggcaatgtc cggcgcgcac tgcggaagca gttcctgctg aatccgccgg gcccagatca
     8041 tttcctgctc gatccgctcc ttttccgaaa gctcttcata caggtgcgca ttgtccagcg
     8101 aaacggaagc gtgctgggcc agcgtctgca ggatcgtgac ctcctcatcg ctgaaaaccg
     8161 ttccgtcctg cttgcggccg atgcggatcg ccccgatcgg tttttcgccg gagatcaggg
     8221 gaatcgccag ttccacggcg ccggtcggat cgctgacgat ctttccgtcc gctttcagga
     8281 actcggtcca gcgctgatgc agggcccgga tggtgagctg gtcgttctgc ggttccggga
     8341 gatgagccgc gccccgttca agctggatct gcagttcctt gcgggcttta tcgtacgtgt
     8401 agatccggca ggcgcgcgaa gtggaaacga ccgaaaacat cgccaccagc gttccgtaaa
     8461 ggcgctcgct ttcaagttcc ttcccgatgc gcacagtcca gtcctgaatg gagtgcagca
     8521 gggatatgcg catcttgaga gaactgatca tgttgttgaa cgtcgaggcc agtgttccaa
     8581 tttcatcatc ggatttcacg ggaacctgct gactcaggtc gcccttgcga atgcgctggg
     8641 cggcggcggt cagcgcggcg atgggagcca gcacccgctg atccagcgtc agcgtaacca
     8701 caatggcaat cagaatcacc ccgcacatga tcagaaacgc ctgtgtgaaa aggatgttca
     8761 ccttctgcca gatgctgcag gggaaggata tttcaacata acccggcaca tcttcatcct
     8821 gcacggcaat ggggccgcgc agatgcacca ttccgctgcg gcggtcgtaa tcaagccgga
     8881 ctttctgggg caggcggtca gcaggaagag gcgcggctcg aggcgggcca aactcctcgg
     8941 tgcggatgaa ttcataaatc ggcgcgcccc gctcatcaaa cacaaccagc cgttccggcc
     9001 ggtgcgtgat ataggcatcc atgtcctccc tgagccggag cattttgttg cggatcacat
     9061 aatccatggc cttggcgctg aatgtgccgc gatgagaatg atggtgggcg gtgagataat
     9121 tgaccaccgc gctgtaggcc atgtaggccg aactgcgctc gcgctcatac atctcccgct
     9181 tgaactgagt gacggtaaga aacgtgatac tcagtccagt cagaataatc atgcatacgg
     9241 cgagcagggt gatcttgtgc gcgaaccgca aatggcggtg agcgggagct ttcagctggg
     9301 gcggcggggg gattgccggc cgctgggcgg gcatcggaat ggacggggac tcctccgtat
     9361 ctatccgcag ttgaacttcg cccattctaa tcgcttcctt gtttcggctg cataacttat
     9421 tccggtgcgc acaaacggca tgtgcccctg aaaagaacct catactacat ctgccggtat
     9481 ttgatgttaa gagaataaaa tggagttttg tacttgaaaa gatggcgtca accgttttac
     9541 ctgtaccgct ctgtttaaat gaacgatgtg aaggagcaaa gatatggcta ttgaaccgat
     9601 tccagttgcg gatattcagg tccctcagga gttcgcgcgc ctgcgcgatc tcgcctacaa
     9661 cctgtggtgg tcatggactc ctgaagcccg cctgcttttc tcaaatattg atgcatatct
     9721 ctggggccgc taccgcaatc cggttgaact tctgatcaac attgaaccgc atcactggga
     9781 ccggctgctc aacggcggcg gcaatttcat gtcgcagtac agaaaagtca tccgcgactt
     9841 cgatcgctac atgaaccccg atgagcccac ctggttccag cagcagtatc ccgaatacaa
     9901 gagcgacagg atcgcatatt tcagcaccga gttcggactc cacgaaagcc taggcattta
     9961 ctgcggcggt ctcggcgtgc tttccgggga tcatgccaaa gccgccagcg acctcggcat
    10021 tccgtttgtg ggcgtcggag tgctgtatcg caaaggatat tttcaccagg cgattgacct
    10081 cgacggccgg cagcaccaca tctacaacaa ttttgacttc taccgcctgc cgctccggcc
    10141 catgcataat gccgcgggcc gcgatcttgt tgttaccgtg gattatcccg atcgccaggt
    10201 gtatgcgcgc gtgtggcagc tcgccgtcgg ccgcgttcag ctttatctgc tggacaccga
    10261 tatccttgaa aatgatcccg gcgaccggcc catgacatcc cagttgtatg tgcgcggccg
    10321 cgaaatgcgt ctctgtcagg agattatgct gggcatcggc ggcgtgcgcg cgcttcggga
    10381 gctcaagata cagcccgccg tctggcacat gaacgagggc cactccgcgt ttcaggtggt
    10441 ggagcgcatg cgcgctcatc tggccgaagg catgacatgg aaggaagcct ccgaggccat
    10501 ccgggtcaac acccttttca ccacgcatac tcctgtgccg gcgggcaatg aggctttcga
    10561 ggcctcgctc gtcgagaagt acatgcgggt tttcaccgat aaaatgggag tggatgtgca
    10621 tcagatgctg gatctgggaa aatcctatcc ggaaggcgga tcacagccgt ttaatctcac
    10681 ggccgtcggc ctgcgtttca gccactacag caatggcgtc agcaagcttc acggtgaagt
    10741 ggccaaccac atgtggcatc acttgtatcc cggcgcaaag ccgacggatc agaacattaa
    10801 atccgtcacc aacggtgttc atatcacaac ctggctggga cacgatatcc ggacgatcat
    10861 cgaagaagct ctgggcaccg gctggatcgg acacgaggac gaccccgaat tctggaaggg
    10921 catcctcaag atacgggaca aggacctgtg ggccgctcat caaagccaga aggacaggat
    10981 gatccgcctt gtgcgtcacc gtctcgtgga gcagtttgcc cgtcacggca aaggtccgga
    11041 cagcattcgg gagatgaacc gcatgctcga ccccaatgtg ctgaccatcg gctttgcgcg
    11101 gcgctttgcc acctacaagc gcgccggcct gattttccgc gactatgacc ggctgcggca
    11161 gatcattacc aatgccgagc accccgtgca gattgtgttc tcgggcaagg cccatccggc
    11221 cgatgagccg gggcaggatc tcatccggga cattgtcacc aaggccatct attctgatct
    11281 caagggctac atcttctttg tggaggacta cgacatccgc attgcgcgtc atctggtgca
    11341 gggcgtggat gtgtggatga acactccgcg ccggccgcgg gaagccagcg gcaccagcgg
    11401 catgaaggct gccatcaacg gcgcgctgaa tttcagcgtc tccgacggct ggtggccgga
    11461 ggcccacaac gggaagaacg gatggatcat cggaagcgaa caccatattg acaacgagga
    11521 tgagcaggat tatcgcgact cgacttcgct gtacgatatt ctgcaaaacc agatcattcc
    11581 gctctactac cagaaccgca ccgccggatg tccgtgcgaa tgggtccagt ggatgaagga
    11641 atccatggcg accatcattc cgggcttcag tgcggcccgc atgcttcagg actatacgcg
    11701 tgacgcctat atgccgaccg cgcagcggta ggttttcatg gatctgctgg tttgccggcc
    11761 cgggtttgaa gacgcgctgc gcgatgaaat cgcgcagagg ctgggcggga aggctgtatg
    11821 tctttgtcct gttcccggca tggttgaggt gcgggcctct gtcccgcttc ctccagagtt
    11881 catttttgag cgccgccgca tgccggatgc gggcttcatt cctgatcagc tgctcaaacc
    11941 cgtaaccgcg gaacaggtgc ggcagatgct gggccccgcc ctccagggaa acttgccgtg
    12001 gagtatttgc tcatttccgg cggggagcgg gacggttggc aatctcggcc cgcgcgtaaa
    12061 gggcattgaa aaggccgttc tgcgcgtcgc gcgggagcat ttcccggaaa caggccggca
    12121 gatgaccgat acccaggccg gcctgcttct tcagctgtgc ctgacccccc gggggcttta
    12181 ccactcctgc gcggcgccgt gtcttcaggc cggatcgccg ctgcgcatgc atcaggattc
    12241 gcgagccccc tcgcggtcct atctcaaact ggaggaggca ttccagttga tgaaccgcca
    12301 gccgcgcaag aatgaaactg cggttgatct cggcgccgcg ccgggcggct ggacctatgc
    12361 gctgatccgc cgggggtgcc atgtgaccgc tgtggatcac ggaccgatga agctcccgcc
    12421 tcatgagccg gggtggggca gggtgcgaca catcagggca aacggcatca cctttgagcc
    12481 cccgcgcgat ctctgccccg tggactggct ggtgagcgac atgcttgtgg ctcccggcgt
    12541 ggtgcttggg ctcctgcgcc gctggatcgg cgcccgtcga atgcgcttct ttgtctgcaa
    12601 catcaaaatc ccccagcaga atccctgtgc cgcggtgaag ccgcttgagg atttcctgtc
    12661 cgcgcagcgc aaggttgaat tcaggctcaa gcagctttat catgaccgcc gtgaaatcac
    12721 catcatgggg cagttgaaat gattttcagg acctgcggga aaacagggaa ggccctatct
    12781 gccatcggcg ccggcacgat gcgtttcaaa acgcctgcga acgacgacga tgtgcgcgat
    12841 gccgcccgtg tgctgctcca cgcccatgaa aaaggcatca actattttga caccgccccg
    12901 tactactgcg gggaccgcag cgaggacatc accgcgcgcg ctattcgcga catgaagccc
    12961 ggatcattct atctgtccac caagtcgggc aagcctgccg gaacggaact cagggcccag
    13021 cttgaaacct cgctgaagcg gctgggcgtg gactgcattc actttttcca tgtgtggtgc
    13081 gtgatggacc ccgcggactg ggttcagcga aaggagaagg gcgctgtcgc cgccgctctc
    13141 caggcgcaga aggaggggct ggtcgagcat gtcgtgttct ccactcacat gaccgggacc
    13201 gaaaccgccg ccgtcatccg cgaaaacatt tttgaaggaa tgaccatcgg ctacaacgcg
    13261 ctcaacttcc cgtaccggca ggagggcatc gaggcggcgg agaaggcaag cattggcgtc
    13321 gtctgcatga atccgctggc cggggggctg atcccggcgc atgcgaaacg gttcaacttc
    13381 atccggcgtg cggacgacgg aaatgtcgtc accgccgcgc tccggttcct gctctcgcac
    13441 aaagccatca cgagcgcgct ggtggggttt gcgaacaaat ccgaagtgga tgcggcggta
    13501 tcggccattg aacagttcaa gccctacagc ccggaggaaa tcaatcagct caagcggcgg
    13561 attgaagact catttgatca gctctgcacc gggtgccagt attgtctgcc gtgcccggca
    13621 ggcgttccga taccgaaata catggactcc tataacatga aaatgctttc cggaaccgat
    13681 gcggcggtgc ttgatcgcct gaaatggcac tgggacatcc aagccgccga tgccggagcc
    13741 tgcacagcct gcggactgtg tgaaagccgc tgcacccagc atctgcccat catcgaacgc
    13801 ctgcgcggca tcagccggat tccagaaccg gggaactgag cacggaacag gcgtcgcatg
    13861 ccgccgggga tgaggaagtc cgggggcgcg gggcgccccc ggggattggt ttttattcgg
    13921 attcgatttt gatctgcttc cgctccgttt tggccttttc ggtgcggggc agtgtgagct
    13981 tcaggactcc gcgcttgaac tgggctttgg ccttttcgcg gtcaatgccc gccggcagcg
    14041 gaatcgtgcg gctgaagtgg ccgtagcgga cttcggagaa gtagcagtca cgctttttct
    14101 cctccctctc ttccttcttg ttcccgccga tggtgacgga gttttcgtcc accgtcactt
    14161 cgatgtcatt ctccgtcatt ccggggagct cggctttcac ctggatttca tcttcggtcg
    14221 atgtgacctc aaaatccggc gaaaccattt tcatgtcgct ccccagtctg ccgagggccg
    14281 gccagcggcc gaaggacggg aaggaatcga atgcctcttc aaacagctcg tcaatctggt
    14341 tgcgcagctg gctgaaagga ttcagtccga agccttcatc aagcctctgc agctcattct
    14401 tccgtttcca cggaacaagt ttgtttttca cgttgctcac ctcccttctg tttacgaaat
    14461 catttccacc ttgatcttct tcggctgggc tttcggcgat ttgggcagct cgaggttcag
    14521 cacaccgttt ttcatgacgg ccttgatgcc gccctgatca atttcttccg agatgcggaa
    14581 gctgcgccgg aaaaccccgg tttcatactc ctggtacacc agctggaaat cgtcgggggt
    14641 tttccagctc tgatgcccgg tcagtgtcag cacgctgtct tcaagaacca catcgagact
    14701 gtcactgctt acgccgggca tgctgcaccg caggaagatc gccttgtcgg tctcatagat
    14761 atccgttgcc gggacaaaga ccgggcggtc ctgcggcagc tccacgctct ggctttcttt
    14821 cttcgcaagg tttctggttt cttgtgtcat ggtgttcacc cctttcatgc gctttcgatt
    14881 ttgatcttct gcggctttga ggcttccgcc cgggccagtt caacggtcag cacgccttgg
    14941 ctgtaggaag ctttggcctt atcctgttcg acttcaaagg ggagccggat cgtgcgggag
    15001 aactccccgc agctccgctc cgccctgtgg cagacggcat ccttgatggt ctcctcctgt
    15061 ttcacctttc cggtgattgt gaggaaatcc ttcagggtgc tgatctcgat atccgccgca
    15121 tcgcatccgg gcagttcggc ggacacgacc gctttctccc cgttgctcca gacattcacc
    15181 gcgaaatcgc tgcggctgcg gtgcgagtag ccgtcgagca ggctgtccat ctcgcgctga
    15241 agcgcccgca gtccggagaa gggttcccat cccgtcattc tgtattccgg caagtacata
    15301 tcattgctcc tttcttattt aatcccatcc cgcacccttg cgggatcatt cagtttccac
    15361 tgccatataa tagcaacaac catgccaaaa aacaaaaaaa gtcataacat attgatatat
    15421 aaataataaa aaaatacgcc aaaccataaa aagagacata attgacacat aaagccctct
    15481 aagaagcgtc aaaatgacac attttgacca aaaatcgtcc caaaaggctc agaaatcgcg
    15541 aaaaatcgtc gaaaatcgga caatatttgg aaaataaggg tgaaaatcag attttcgggc
    15601 ttggattaca aaaaatccaa gccgggccgc gaaaagattt ttttaacgca gcagggccgc
    15661 aatggatgta aaaacaaaca gaaagccgct gacccgaata atctgatgaa ccggccgaat
    15721 ggccgtgctg actggatgca taaagagtgg agcgggagac ggggatcgaa cccgcgacgt
    15781 tcagcttggg aagctgacat tctgccactg aattactccc gcatgaggtt ttcagttctg
    15841 aacgcgataa catgtatcag gagagtctgc atttgtccag tgccgtcccg gctgtttttt
    15901 cacaagtttg gaaatgtcgg gaaggaatgc tgcgctattt tggtatagac attaaaaaaa
    15961 gtccactctt gcgggttcat ctccggcctc aaggcatctg ctcaggatca gcctcgatca
    16021 agacagatat gttttctgcg gctgaccgcg gggccggatg cgcaatatct tgacagccgg
    16081 cgccatgcgg ccgtaaattc gcgcgctgaa gtaatattca cgaccagatg aggaacgtgc
    16141 cgacagggag aaattgttcg agggagggct tcccgtgccg gtctggccgc gtttattgag
    16201 aaagaggccc ctgtgataaa ttcagcatcg aatgaacggt gttccaccat gtcctccagc
    16261 ccggcgggaa agcgattggc gaattcagca ggtattcttt atggaatggc tctgttgctt
    16321 tttgccctga tggcttcccc ggcacgggct cagatgctcg agtcgtttga atacggtgtt
    16381 ccgcctcccg gctggaccaa gacaaatctg ctgggcggct cgggatggta tcgcctgccg
    16441 gttggagtca tgcccctccc gggatggggc aatgggacga gttctgtccc gcccacggcg
    16501 aatgcgggtt cggccaatgc ctactgctcg tggaccaccg gaggcggtgc ttcagacggc
    16561 taccacagcg accagtggct gatctcgccg cagctgaacg gattgacggc gacatcctcc
    16621 ctgagctact ggctgcggtt cgcgttcacg aatttcccgg acacggtgta tgtgcgcatc
    16681 tcaaccgccg ggcccgcccc ggcgaacttc acgcgcgtgg tctatacaaa tatctggtcg
    16741 agggggggca atcccagtgg tcagtttggg ccctggaccc ggtacgatgt gaatctcggg
    16801 gcgctcggca ttgctgcggg aaccccgatc tgggtggcgt tccaggaata cgagtgggac
    16861 aacattcata acggggcggc cgtgcagctg gacgtgattt ccagcgactt gaccgcgccg
    16921 ccgcagccgc gcatcagtcc gacatcgctg acgttcacgg cgtattgcga aggaagcgat
    16981 ccggccccgc agaccttctc cattcagagt atcggcagca gcgggatgag ctattcaagg
    17041 accttcgcct tcggcgccgg gcccaccaat tggctgactc ccggcggcct gttgtccgga
    17101 acagttgcgt ttcagaacag ccaggtcttt acggcgtcgg tgtcggctgc cggcctcgat
    17161 ttgggcacgt actgcgcaac caatatcatc acggtcccgg gggcgaccaa cagtccgctc
    17221 cggattccga tcaccttcca cgtgatccgg cgcccgcaga cactctcgtt tcctaatccg
    17281 ggcgcgcaat ggaccacaaa ccgcgtggga ttggccggca cgtcgagttc cggcctgccg
    17341 gtgaccttca gcatctattc cggtcccggt gtggtcacgg gcgccactaa tctgaccttc
    17401 acgggcacgg gaaccgttcg agtgatcgca tggcagctcg ggaatttcta ctatgacgct
    17461 gcgccgtgcg tcacgaattc gatcacggtt tccaagcccc atgcggtcat atcattcacc
    17521 aacatgagcc acgtctatga cggatctccg cacggagcca cggtcatcac tgaccccgag
    17581 ggcctgaccc ttgtcaccac gtataacggg agttcatccc tcccggtggc cattggaagc
    17641 tatgcggtca ccagcactgt caatgacgcc atgttcgacg gcacagcggt cagcacgttc
    17701 acgatcacga gaatggtcca gagcgtcaca ttcccggacc tcggacctca atggaccacc
    17761 agccgggtcg ggcttgcggc aacggcaagt tcaggactgc ctgtgggttt ttccatcgca
    17821 tcgggcccgg gttccatcag cggcggaacc aacctttcgt tcacgggtgc gggtgctgtc
    17881 agcattgacg cgctgcaggt gggcgatgcg gattggcttc cggcgtcggc cacccgcacg
    17941 atcgaggtcg caaagacaga gacatcattc attttcaatg acttgagtca aatttacaac
    18001 ggcaacgcgc gggttgtcac tgcaacaact gttccgccgg gattgaatgt tgaaatcacc
    18061 tataacgggg ctgccccggc cccgattgcg gccggcagtt acactgtgac gggggtcatc
    18121 aacgatgtca tttacagcgg aatgagtgtg gccacgctga cggtcggccg ggcggaccag
    18181 gtcatcacgt tcccggacct gtcgccccgc acaacgaatg aagccgttgg attgagcgca
    18241 agcgccagct ccggcctgcc gctgacggac ttcgccgtga tggccggccc cggcatggtc
    18301 aacggaacaa acctgacctt cagcggcgcg ggcgatgttc tggtcgcggc gctgcaggcc
    18361 ggcgatgaaa actggaatcc cgcggcggcg gtgacgaatc tggtgaaggc gttttcgctg
    18421 gttcccaata atggtccggc ttccggcgga aacacggtcc tgatcacgaa cggccgcatc
    18481 ggcgacggcg ccggcataac gaacgccacg ctctgcggga tcgcggcgac gattctcgac
    18541 cagggcacga actgggtgag tgtgcagtgc gggccgggcg gcgcgggcac gggcagcatc
    18601 ctgctccagt cggccagcga tatcgttgtc agcaatgcct acacatacaa cccggccgga
    18661 accatcttcg cggtggcacc tgccgtgggt tcatacgttg gcggttatcc ggtcgttatc
    18721 acgggcacca atttgtgcag cggcgatgtg actaatgtta cgctgtgcgg cgcgagcgct
    18781 gcggtgagca gccaatcgcc gactcagatc atcgtgaccg ccggggcggg agcccttggg
    18841 ttgggcgatg ttcgcgtgtt ttccgtgagc ttcggtgaaa cggtcttgtc caatgccttc
    18901 acgtatgccg ttccggagat gacggttctg ggcgccgggg gcgaggtggt aggcagcgga
    18961 gatgcggccg ccctgtccgc gggcacagac ttcggcagtc aactggtttt cgatgcggca
    19021 acgaactggt ttgagatcac aaacagcggg gctgcaagtt tatatatttc aagcgtgacc
    19081 accagcggcg caggcgcggc tttctttgcg gcctgcaata tgccggccgg tattcagccg
    19141 ggcggggcag cggggttctc aatcgtttat gcgccgacaa ccgttggggt gcacagcgcg
    19201 gcgctctggc tcgtgaacga cagcacaacg acgccgtacc ggatcaatct ggcaggcgct
    19261 tcgctgaaga aggatcagca cgatctgacc ttcaccccgg ccacgccgca aaccttcaat
    19321 acaacaaacg ggctggctgc gggcggcgga cagtcgtcgg gcggctggac ctacacggtg
    19381 gaatccggcg cggcggagat cgccggtgaa acgaaccttt ggatgcggac cggcaccggg
    19441 agcgtgacgg tgcgcgcgac gagggcaggt gacgcaaatt ggaacgaggc ctggatcgaa
    19501 gccgttgtaa acgcggccag ggcatcgcaa acgatcaatt tccctaatcc gggcacccag
    19561 agcgcgacga atatcgtgga cctcagtgcg gacagttctt ccggtctgcc cgtgatttac
    19621 ggcgtggtgt ccggcccggc ggtgttcacc ggcacaaacc tgaaccaggt ggcttttaca
    19681 aatctgggca cggtggttct gagtgcgtcg caggccggaa atgccgactg gaatccggcg
    19741 gcaacaagcg tgacatttga ggtggatcgg gttcctgcgc aaattgtgtt cacgaacctt
    19801 tgctatgtgt atgacggaac tgaaaagacg gccacggtga gcacgatccc gccgggcttg
    19861 aatgtggtgg tgacctatcc gcccctgatg atgccgccgg tgaatgcggg aacctcgcgg
    19921 gttgaggcgg tgattgagga ttcgttgtac cgcgggagcg atacgaactg gctggtcatt
    19981 gcgtgcgcaa cgcagggaat cgggtttgcg gcgattgcag accaggccat caccagcacg
    20041 ctggagcttg cgggaaccgc atcgagcggg ctgccggtga gttttgcggc atccggcccg
    20101 gcggtcctct ccggcggaac caatctgagt ttcatgggaa ccggtctggt cagcatcgct
    20161 gcgtcacagg agggcagcac gaattatctg cccgccgcgg tcacgaacag tttcctggtg
    20221 tatcctctga gtccgctggt ttccggaccg ttccatctcg gcgcggcggt gaccggcgcg
    20281 ctgatgggcg cgaccataga aaatgtgtac ggggccaacg ctttccagcg cggaattgtt
    20341 tggagccccg ctccgggttt tgatcctgtt tcggccagcc gcgcagagga acttggagac
    20401 tttggcgccg ggacctggac gcagcaggtc agcggattga gcgccggcct cacaaacttc
    20461 tatcgcgcct atgcggtcaa tacagccggc accggctata cggctgaggc gtgggtgctc
    20521 atgcgtcccg ccgcgccggt ggctgaggat gcgtcagatc acaatcctga cagtttccag
    20581 gccaactggc agttgacggc tggtgcatgg acctattggg ttgatgtatc cgaagatgcc
    20641 ggttttgcat cctttgtgcc gggttatggg ggccgtccag ccggtatcgc aaattccatt
    20701 gtggtgaccg ggctgacttc cggcgtgacg tatcattatc gcatccgcgc tgaaaacgat
    20761 gccgggctga gcgactggtc gaatgtcaaa ggatgcatga cgcggcctct gctgaccatt
    20821 gtgtgccagc ccggaagcgg agggacaacc gtccccgcgg ccgggaccct gaccgtggat
    20881 gccggcgatc ctgtggccat ccaggcccgg accaattccg gttacgcttt cgtgcgctgg
    20941 gatcagattg gcggcggggc tgtggccagt ccgctggcat ccaccacaac ggtggttctg
    21001 gtatccaatt caattgttac cgcggtgttt gcgcagcagg cggctggcgc cgcagcggtt
    21061 attgaatcgt ggcagaccaa ccggctgtcc ggcacacttc tggccagcat caggatttgc
    21121 aacaaccaga ccaatggagt gcggatggcg gagcctttcc atttttgtgt tcaggccacc
    21181 gcggcataca gattgatgca tccgtctgga acgaatgtgc tcacgaaatg gccgtatgcg
    21241 gatatcacgg cgcagattaa agccggagcg ggcgacggca ttctcgaccc gggtgagtgt
    21301 gtgctggtga ccaacatcga gttctttgtc aaaacactgg tcgcgccggc cgctttcaac
    21361 tggaaggtcg tcgccgttct gctgccgccg gcggccgcga tggataccga tggggacggc
    21421 attcccgacg tctgggaaca gatgttcccc gccaccctgg attgtctgtt tgcgccggat
    21481 gccgggattg actccgacgg ggacacctat gacaaccatg acgaatacac ggccggcacg
    21541 gatccggaag atcccgcttc ctatctgaga atatccgccc tgaccaatgc atttggaatc
    21601 ggcaggacga tccaatggcc gggagaaagc ggcagatatt accgggtgtg ggggaccacc
    21661 aacctgctgg atgcctacca ggtgattggt tcgggtgttt ttgcaacacc accggtcaat
    21721 gttttcaccg acgcggtcct ggacgtgcag aattccgcct tctacaaaat cgaggccgga
    21781 ctgacaccat gacgggctga tctgccggcc gataaaatcg cgccgtctgg attttgtgct
    21841 gcctgaaaag gggcctgaaa acttcaaaaa aaaggctcaa aagcagaagc cggcctctga
    21901 gctggccgga aaaagaatgc gatgggaatg gggacgggac ccgacttcgc ccagaggctt
    21961 cgtcgggcga acagagcccg tccctccagc agcggggggg ggtgttgttt gtgccgaccc
    22021 ccgcgacctt gtgtcgcggg tgaggaaagg tctgcgggtt ccgccgtatt gtgcctgatc
    22081 tttcgctccg gcgggaccag cccgccgggg gcaataagga tggggtacac ccgccggggg
    22141 cgcatccgga agcagacaag ccagacgtcc catggattca tctcggtatc cacttgattt
    22201 ccgcggggcg ggccgcatac tctagcgcgt ttttgtttgt gagagaaacg gagcggagac
    22261 gatg
//
LOCUS       DAOQWR010000025        22037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2717_length_22037_cov_5.401146, whole genome shotgun sequence.
ACCESSION   DAOQWR010000025 DAOQWR010000000
VERSION     DAOQWR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22037
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_22037_cov_5.401146"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..139)
                     /locus_tag="PKK36_03495"
     tRNA            complement(50..139)
                     /locus_tag="PKK36_03495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:cga)
     gene            395..850
                     /locus_tag="PKK36_03500"
     CDS             395..850
                     /locus_tag="PKK36_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNR93656.1"
                     /translation="MKTKRHLSASLEDYLETILNIEAEKKAARVKDIADRMHVRSASV
                     TGALRTLAKRGLVNYAPYDIVTLTPAGEAVARRIARRHRALNSFFRDVLCVEEQVAEE
                     CACRMEHMVSDSVMERFVSLAELLESGPCGTVKWDARKHGFVFRKGRES"
     gene            847..1089
                     /locus_tag="PKK36_03505"
     CDS             847..1089
                     /locus_tag="PKK36_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNR93657.1"
                     /translation="MTANTQQELRKLSKVTSGQQVELVRVESGQAMQSRLAAMGLVPG
                     TCFKVIRNDSRGPCVIGLKGTRLVLGRGMAERIEVR"
     gene            1107..3536
                     /gene="feoB"
                     /locus_tag="PKK36_03510"
     CDS             1107..3536
                     /gene="feoB"
                     /locus_tag="PKK36_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNR93658.1"
                     /translation="MAKTIRAVLAGNPNSGKTTIFNNLTGARHHVANYPGVTVSTEQG
                     EFVVGDAKFVITDLPGTYSLTAYSPEELIARNFIIREKPDVVINVVDATNLERNLYLT
                     TQLMELGVPLVLVFNMSDMAHQHGAGIDIARLSALLRVPVVETVGHKRRGMEDLCRAA
                     LDVAEGRREGGGVPLHYGHDIDPAIAAITAEIEADANLVLEVSARWMAIKLLENDPRV
                     RERITQPAILEMAARHRAHLAQLLGDQPETVIAGRRYGFISGACQESLLPSSIEARHI
                     QSDKIDTVLTHRFWGLPIFLALMYVVFAVTFNVGNYFVGWLESLFGWLGGTLSGFWPE
                     GSGSLLESLLVDGIIGGVGGVVSFLPNILLLFMAIAFLEDSGYMSRAAFIMDHLMHRI
                     GLHGQSFIPMLIGFGCSVPAILATRTLDNRRDRITTIMIIPLMSCGARLTVYALIIPA
                     FFPVRWQGPVLWLIYFTGIVVAVGAAWVLRRTLLRGETVPMVLELPPYRMPTLRGVAV
                     HTWERSWEYVKKAGTVILSISIVLWAMTAFPRRPEADGTYAREIAAAHGSYEQGVREL
                     AGRLALPEDTDLAALLEKPPRDESGIADPHLAHFFSLLRQKRAIERRFAPHHHREGEL
                     HSDPACLQKELMLTHLRSADPAAYRLVAHYDDKVFAPREQILQQIERKRHADGLAYSM
                     IGRIGRALDPVWKPMGFDWRIGTALIGAFAAKEVFVAQMGIIFAVGGDEDSADALRRQ
                     LRATYPPLVGLCILLFTLISSPCLATVAATRKETGSWGWALFQMAWLTALAYAVTAAV
                     FQIGSLAGWGV"
     gene            3540..3710
                     /locus_tag="PKK36_03515"
     CDS             3540..3710
                     /locus_tag="PKK36_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93659.1"
                     /translation="MLENILVAVIVGLALGFIIRGAIRRARGKDSLCAGCPMAGRCGD
                     DARSQMKCGIRK"
     gene            3707..7552
                     /locus_tag="PKK36_03520"
     CDS             3707..7552
                     /locus_tag="PKK36_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNR93660.1"
                     /translation="MKTSFSAIWTALLLAGAANVCAGTIPELLDRAAALEAAAGVDSG
                     GYPDAEEVLVAGIQKIAYEADGTYVQWHEEYVKILTEESRRSHLTLSSFFTIPYQRGP
                     EDCQIALVEIIRPDGTVIPVDVEAESRVMVNPSSMEDNIYNPNDQLIQVNISDLQIGD
                     VLHYVLFDRIVKPRVEHTWSDWLVFEGLRPMLKQVVEISGPARMPLQSIAHKSEIPGT
                     VVFEERTDGDRKIWRWEARDVPRMFPEPGMPAPHTVIQRVLVSTIPDWETISRWYWKI
                     SEPHFKAGPALEEKVRELTDALTTPADRTWALFKFVSQEVRYMGITKEAESPGYEPHD
                     VMDTFEARHGVCRDKAALLVAMLRLAGLDAYPALIHNGPKLDSEVPQPFFNHAVVAVK
                     NEDGGIILMDPTDETTTELLPAYLSDKSYLIASPEGEPLRTSPIVPADQNMMTIITTG
                     RVDRAGTLFADTVMEFRGVNDNAYRGLFARRKPEERQQFFEGLAKSMAPGAAVGRFSI
                     EPADMTDTSTTLTVRISFTAPDLLIGREGTAALPLPQAGPRLGVANFIVGQTGLQTRK
                     YPLVTGMACGVDERVRIELDPSLGEAEALPRGTPQDSDTLLWSMESSMEGRIVDAAAR
                     FVLKGVEFSPAEYLDLKKTLKQMEMDYRKMPILGRVEAGGADASRGAAAEEEPEVEAD
                     SEVLNMTLVYELADTGRWTEISTVKERILTYAGKKNSSEIKIGYNEAWESVELEYARV
                     TAAGGAVREISTNEINVMDAPWVGAAPRYPKGKILVISLPAVEVGSVVEYRIRRECRD
                     RPFFSAAAHLRATRRIQQMTVRLSAPEDLPLHLLSENISGDQVRHSSRAQGGRQFHKW
                     ISGPAEPVRQEDSLPPWWTFNPSLFASSGNWSGYAAALHELFTAAAEGQTQTVEQAAI
                     ITAGTDDPQVKLERIRDFVALNNRRAGPGLGVIPLSSITPADQTLRSGYGNSPDRAIL
                     HYALLKAAGFDPEFVLVSSLPTIEEVSRPYREIPAMHAFPEVVVRLRDRLPDVEPGRH
                     VYFNDTTQYDAVGASGNEGLLGITLPDAEFCEILPAKPTRMETVSRMDLQEDGSAVIS
                     RKILLYGSNFGDNHQLFAEMQPEERRRYFRELIASVSQAAEPEGDLFTDFGRYPGIVE
                     YAVWVPDYAVIDEGFFYFPLPDTPARILPMRADRRVNPFYFPDETVRSLRMDVTWPEG
                     YAVKLAPESMQEDGAAGHPVALRLQSGPAAEETAWRIESTVTVKPVVVPASDYAAFLE
                     LDRKMAHRRNRFMLLRREE"
     assembly_gap    7753..7852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7999..8907
                     /locus_tag="PKK36_03525"
     CDS             7999..8907
                     /locus_tag="PKK36_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR93661.1"
                     /translation="MAAKHVGLGRGLGALIKEVPAAPKADEKDAPAGGITRIPTDSIR
                     KNPFQPRHAMEQEPLADLVRSIREKGILQPLLVRPASDGYELIAGERRLTAAREARLR
                     EVPVIIMSIDDREALEIALIENLQRENLNVIEEADGYHELCEKFGMTQEEVAARVGRS
                     RAAVTNTLRILGLPDDVKQFLSKSELSAGHAKVLLGLEIPQEQSLLAKRVVKEGLSVR
                     ALEKIVNRARRTPKKARTRRSDIPEEHLRYLSNRLHQHFGTAVNITPCKTLPNGKKSK
                     GSLEINFYSSDELDRILAMIGITEDL"
     gene            complement(8909..10120)
                     /locus_tag="PKK36_03530"
     CDS             complement(8909..10120)
                     /locus_tag="PKK36_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNR93662.1"
                     /translation="MIRILHLGPRSAVRAAVRLLLLALAVLFAFGGPLPFWMKRIFPS
                     FSPLSLIASALAQRGWYAGVFWMTPPLVVLLMGLFRGRMFCNWICPAGTLFHLASPRK
                     CIAHVSRRRWSGYIFWIIIFSSLAGFPLLFILEPQPLLQRDLLWTGRGLNALALVPGL
                     VFPLFLLLGIFQPMIWCTHFCPAGHFFDFLRHLRKSPAQTFSKERRELLAGLCIGLPA
                     ACAAAHLPGFKTKNKWMPALPPGAHDADQFGAICHRCYACVDSCPTGILRAGFRVDRP
                     LTSWFQPEMNPEFGACEEFCDNCSRACPTGAIKKTSVDEKRMIQIGIAEVNREACLAW
                     TDGEHCMVCDEYCPYNAIETSFSETGIPRPIVNPEICRGCGFCQHACPAVRMGRAIIV
                     RGLEKQRVLEG"
     gene            complement(10129..11052)
                     /locus_tag="PKK36_03535"
     CDS             complement(10129..11052)
                     /locus_tag="PKK36_03535"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR93663.1"
                     /translation="MYLRQTTRRTFLKQAFASGAALTLGPSWLALAEDRSPVEGPDVW
                     VIHGGDKRALIGKALEIMFENGGFGPQPDTLTLKVNAAWARTPDEGACTHPELVTAFL
                     EGCRDAGVKKTVIPENPCNDAAYAFTRSGLQDAAARAKTPMINLEKEKKYWKSFELPQ
                     GRSLKTAKVASQFMETDVLVNMPVAKHHGGATLTMAMKNWMGAVADRGWWHRNDLHQC
                     IADCCTLIKPHWTIIDATRIMLSRGPQGPSKDMKYPNLLVVSKDQVAADSFTSTLFHD
                     SIEAVRYLTIARDMKIGETDLAKMNIHRVEA"
     gene            11237..14377
                     /locus_tag="PKK36_03540"
     CDS             11237..14377
                     /locus_tag="PKK36_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNR93664.1"
                     /translation="MGRLKRGVWLLICACAWGHACADGRAADSPFALTAEEQAWLTQY
                     PAIRIGIMTDWPPLDFCDVDGKPSGIGADFLNELNRRLGGIIQIECGTFDDNIREVKT
                     GELDGLMDVTPRPEREEFLNFTRPYLTIPHIIVGRKNGAYYSSASELKGRTVALERGF
                     GNVTWFQENYPEAQIRLYDSTSSALDAVSRGEADAYVGNRAVALFLIEREMLTNLQLQ
                     GRAEKPPVLLTLGLRKDWPLAIHMLDRALAEIIRTDERFILSKWFDVASRAGLQLQLT
                     ADERAWLQAHGEIRIGINNNWPPMDFVDESGNPYGIGTDFINLLNEGLHGRIKIVPGP
                     WEQIYRDCLENRLDAVMDITPREDRLEFFNFTKPYVNIPHVIIARQHTGYFDAFASLS
                     GKTVAVEKAFFVGQHIRKEHPGISILEYPSTREALLAVSEGRADAYVGNRAVATWIIA
                     RELLSNLEIQGTVVETSSINAIGVRRDMPELAAILDRALISLPAKSVHSIYERWGGGG
                     EAVSLTWMGLSTPEKAWLNEHSVIRVGSRKRIAPLEYIDKFGVPQGISWDYLRRFEKM
                     LGVKFEFVDLGDWSEAEAALRAGQVDMLTATSDSQISAEGLAFTQPYLSMQTAIFTHQ
                     DTPYIGQIADLKDLRVVAVPGHSSTVNIQREVPDITLMRAKDMPEALRMLENRKADAF
                     VGTLLVTSHYIQEGGHTRIKVSGESAFVFHPAFVCRQDWRVLTDILSIILADIDVSEQ
                     NAILRKWTTVTYEQRINYSLLYRIVGSVLVLLAVFLYWNRRLAAEIRRRRQVEEVLRR
                     SEVQLIEAKERAEAADQIKSAFLANMSHELRTPLNSIIGFTGIILQELAGPLNSEQKK
                     QLEMVRNSSQHLLALINDVLDISKIEAGQMDITTEPFLMPDAVRAAVQTVLPLARKKN
                     LPLHVDIARDVGEIVSDRRRVEQILINLINNAVKFTDKGSVTVSCRMENGQIRISVRD
                     TGMGIRPENQGKLFKAFQQIDSGTTRQYEGTGLGLSICRKLLEMMGGTIAVESEYSRG
                     STFSFTLPVNKEKMNP"
     gene            14374..14745
                     /locus_tag="PKK36_03545"
     CDS             14374..14745
                     /locus_tag="PKK36_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93665.1"
                     /translation="MNILVIEDNDQNMYLTEFLLSRHGHSVLKASNGADGIALARQSR
                     PDAILLDIQLPGMDGYEIAGVLKNDEVTRHIPVIAVTSYAMRGDREKVLAAGCEGYIE
                     KPINPDTFMKQILSFIPGGQK"
     gene            14742..15557
                     /locus_tag="PKK36_03550"
     CDS             14742..15557
                     /locus_tag="PKK36_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93666.1"
                     /translation="MKTILIADDIEANRYLLQSLLKGSGYKVSTTQNGREALDAARKN
                     PPDMIITDILMPEMDGFQLCRAWRTDKALAGIPFIFYTATYTDPSDEKFALDLGADRF
                     ITKPQSPETLLQAIQAVFEETETKGVHAAASGVSTGDQYSQVLFKKLEQKVQDLEETR
                     GKLEKEIRDRQQAEDERIQLERQLRQAKKMEALGALSSGVAHDFNNILQGITGYAELA
                     LDALPDAPPGGPRLSRGNHPRRRTRLGADPPDHDVQPGSQGRPDSVSAGSRRP"
     gene            15493..16419
                     /locus_tag="PKK36_03555"
     CDS             15493..16419
                     /locus_tag="PKK36_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR93667.1"
                     /translation="MTFSREVKVGRTPCLLEAVVREAINLMRGKMPPRVVMDADIISG
                     GPHALVDAGQIHQIITNLISNAFDAMREGGGHVQVSYYEEDVDEHKAVQAPRLKPGRY
                     AVLQVADTGPGMDPATLERIFEPYFTTKAAHGGTGLGLAVVHGILETHGASISVESRP
                     GLGTTFTIYFPACQLRDNGRDVGPELRGSESILLVCRLNEQSNGNPGMALGGLGYQVR
                     PCSGEAALQEVRSEQSGPAAVLIDCCTPGINPASLTAQFKQMAPELPVVMYYSAPVKP
                     GEESDIPLGIHFVADQPASLKDLAAEIRALLA"
     gene            16529..17881
                     /locus_tag="PKK36_03560"
     CDS             16529..17881
                     /locus_tag="PKK36_03560"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNR93668.1"
                     /translation="MKVVKFGGSSVASAEQISKVIRIVTSDPDRRIVVVSAPGKRSKT
                     DTKVTDLLIALAGSVIANKNHEADLKAVVDRYAEIQRDLQLSPQVVETIEYDLRGRIA
                     KRGKNDAQFMDAMKAAGEDCSARVIADAFTKAGFPAKYVNPGDAGMLLSDDFGNAQVL
                     DESYPKLAQLKDVKEIVIFPGFFGVTKKGDVATFPRGGSDITGAILAAAVKADVYENF
                     TDVDSVCAMDPRVIENPPAIDVMTYREMRELSYAGFGVFHDEAIVPAVHAEIPICIKN
                     TNNPSAPGTMVVPTRAPNERTVVGISSAPGFCAIYIDKYMMNREIGFGRRLLQILEDE
                     KISFEHTPSGIDSISVILASANIPPAKEERVVGRIKAELKPDAVVVEHGLALIMVVGE
                     GMHYAVGMAAKATRALADAGANIEMINQGASEISMMFAVKDTDREKALRSLGRAYFGI
                     "
     gene            complement(17936..18802)
                     /gene="speB"
                     /locus_tag="PKK36_03565"
     CDS             complement(17936..18802)
                     /gene="speB"
                     /locus_tag="PKK36_03565"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNR93669.1"
                     /translation="MNELTYPSFLESDMDPPLPENAKFHILPVPYEKSTSYGEGTVRG
                     PSAILNASQQLELFDGHSIPAEEGIYTHLPVDCDLPEETVLAEIAEQIGKIHHAGHIP
                     VMLGGEHTVTYGAIKALHDAGEKFGIIQFDAHADLRSEYEGTKWSHACVMRRAVEMGI
                     PLCQIGVRSLSLEDLEIRVENAIMHYDMEETVEKGLDMSLVLDNFPEKVYVTFDVDAL
                     DPSIMPGTGTPEPGGMQWYPALKILNHIARRCEVIGFDVVEVSPMAGSNISEFTAARL
                     AYNFMGMIARRE"
     gene            complement(18814..19992)
                     /gene="nspC"
                     /locus_tag="PKK36_03570"
     CDS             complement(18814..19992)
                     /gene="nspC"
                     /locus_tag="PKK36_03570"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HNR93670.1"
                     /translation="MKESLRSLRSFAVNSPWLKSVETPCFVVFEDKLKANLAVLDTVQ
                     KRTGCTILMALKGFAMFRVFPLIRQTLRGVCASGLHEARLGREEFGGEVHVFSPAYSA
                     ADLAELVKIADHLVFNSFSQWHLARDIIAKSGRDISAGLRVNPEHSEAKVEMYDPCAR
                     YSRLGIRFTEFEEQDLTGISGLHLHVLCEQNADALVRTLVSFEDRFEKYIPKMKWINF
                     GGGHHITRDDYDVDLLCDLITRFREKYGVQVYLEPGEAIALNAGVLVSTVMDIKRNEK
                     HIAILDTSATTHMPDVLEMPYRPAIRGAEKPDHFDYNFRLGGPSCLAGDVIGDYSFEK
                     PLQPGDRLVFEDMAHYTMVKTTTFNGVPLPSIYLCASENAEPELVRKFGYEDFKSRLS
                     "
     gene            complement(19989..20375)
                     /locus_tag="PKK36_03575"
     CDS             complement(19989..20375)
                     /locus_tag="PKK36_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR93671.1"
                     /translation="MDEIKRLCDQVREIAYAIHVYHGNGYLEKVYENALVSRLRKEGL
                     SIKQQQPITILDEDETVIGEYFADIIVNDCLLVELKAAKSMAIEHETQVLHYLKATGI
                     EHGLLINFGSYKFEIKKYIRTQGASQ"
     gene            complement(20422..21603)
                     /locus_tag="PKK36_03580"
     CDS             complement(20422..21603)
                     /locus_tag="PKK36_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNR93672.1"
                     /translation="MAKVLIVGAGGVGAVVAHKCAQVPEVFTDITLASRTKSKCDAIA
                     AQISRPIHTAQVDADNEKEMADLIRRTGAEMVINVALPYQDLHIMNACLETGVNYLDT
                     ANYEPPDEAKFCYKWQWEYQERFKKAGLMALLGSGFDPGVTNVFAAWAQKHHFDEIHD
                     LDIIDANAGSHGQPFATNFNPEINIREVTARGRYWEKGEWISTDPLSVSETFEFPEGI
                     GPMKIYLLYHEELESLAKNIRGLKRARFWMTFSDNYLKHLEVLQNVGMTRIDPVKFEG
                     HDIVPLKFLKALLPDPASLGPLTKGRTCIGDLMTGVKNGKPKKLYVYNICDHEACYRE
                     VQSQAISYTTGVPAMIGAMLMLTGSWKGRGVFNMEQLDPDPFMDKLNKHGLPWTAVEL
                     K"
BASE COUNT         4533 a         6598 c         6582 g         4224 t
ORIGIN      
        1 cttcccgcgc ccgtggtcgg tcgtagctca aaatctgaaa atcaggaatt ggcggagagg
       61 gagggatttg aacccccggt aggcttgcgc ctacaacgga tttcgagtcc gccgccttaa
      121 gccgctcagc cacctctccg tggaaatgcg tgcaggctag aacagagccc cgctccgctc
      181 aagccctaat tcgctccagc ctgtgcaaaa aaataagaac agtgcttatg ccaaggaggt
      241 ctgggtgagg aacccatgag gaagcataag cactgttctc atgatcagca tacatgacca
      301 tggggaaatt gcaagaggcc ggccgcattt ttttgcaata gagattggca cagctcatat
      361 tgttagtgta ccctaacaac gatcttgaat aatcatgaaa acgaaacggc atctcagcgc
      421 aagtcttgag gactacctcg aaacgattct gaacatcgag gcagagaaaa aggcggcgag
      481 ggtgaaggac attgcggatc gcatgcatgt gcgcagcgcc tccgtcacgg gcgcgctgcg
      541 gacccttgca aagcgcgggc tggtgaatta tgcgccctat gacatcgtca cactgacgcc
      601 ggcgggcgaa gccgtggccc gcaggattgc gcggcggcac cgcgcgctga acagcttctt
      661 ccgcgatgtg ctctgcgtgg aggagcaggt cgcggaggaa tgcgcctgcc ggatggagca
      721 tatggtttcc gacagcgtga tggagcgctt tgtgagcctg gccgaactgc tggagtccgg
      781 cccctgcggc actgtgaaat gggatgccag aaaacacggg tttgtcttca gaaaaggcag
      841 ggagtcatga ccgcaaacac acagcaggaa ctcagaaaac tttccaaagt gacgtccggc
      901 cagcaggtgg agctggtgcg ggtggagagc ggccaggcca tgcagtcgcg cctggccgcc
      961 atggggctgg tcccgggcac ctgtttcaag gtgatcagaa acgacagccg cggaccgtgc
     1021 gttatcggcc tcaagggcac acggcttgtg ctggggcgcg gcatggccga gcgcatagaa
     1081 gtccggtaat attccgcggg tgatttatgg cgaaaaccat cagggcagtg ctggccggaa
     1141 atcccaactc gggaaagacc acgatattca acaacctgac cggcgcacga catcatgtgg
     1201 caaactatcc cggcgtgaca gtcagcacgg agcagggcga gttcgtcgtc ggcgatgcaa
     1261 agtttgttat tacagacctc ccgggaacct acagcctgac cgcctattcc cccgaagaac
     1321 tgattgccag gaatttcatc atccgtgaaa agccggatgt ggtcatcaat gtggtggatg
     1381 cgaccaatct tgaacgcaat ctctatctga caacccagct catggagctg ggcgtcccgc
     1441 tggtgctggt gttcaacatg agcgacatgg cgcatcagca cggcgcaggc attgacatcg
     1501 cgcggctttc cgcgctgctg cgcgtgcccg tggtggagac ggtcggtcac aagcgccggg
     1561 ggatggaaga cctctgccgc gcggcgctgg atgtggcgga gggacggcgg gaaggaggcg
     1621 gcgtcccgct ccattacggg cacgacattg atccagcgat tgccgccatc accgccgaaa
     1681 tcgaggcgga tgcaaatctg gtgctggaag tgtccgcgcg ctggatggcg atcaagctgc
     1741 tggaaaacga cccgcgggtg cgcgagcgaa tcacgcagcc cgccatcctc gaaatggcgg
     1801 cccgccaccg cgcgcatctg gcccagcttc tgggggatca gccggagact gtcatcgccg
     1861 gtcgccggta cggattcata tccggggcct gccaggagtc gctgctcccc tcctccatcg
     1921 aagcccgaca catccagtcg gacaaaattg atacggtgct gacccaccgc ttctggggac
     1981 ttccgatttt ccttgcgctg atgtatgtgg tgttcgccgt cacgttcaat gtcggcaatt
     2041 attttgtggg ctggcttgaa agtctcttcg gatggctcgg gggaaccctc agcggcttct
     2101 ggcccgaagg aagcggcagc ctgcttgaat ccctgctggt ggacggcatc atcggcggtg
     2161 tcgggggcgt cgtctccttc ctgcccaaca tcctgctcct gttcatggcg atcgcctttc
     2221 tggaggattc gggctacatg agccgcgcgg cgttcatcat ggaccatctg atgcaccgca
     2281 tcgggctgca cgggcagagt tttattccca tgctcatcgg cttcggctgc tcggtgcccg
     2341 cgatcctggc cacgcggacg ctggacaacc ggcgcgaccg catcaccacg attatgatta
     2401 ttcccctgat gagctgcggc gcgcgcctga cggtttatgc gctgatcatt ccggccttct
     2461 ttcccgtccg gtggcagggg ccggtgctgt ggctgatcta tttcaccggc attgtggtgg
     2521 cggtgggcgc ggcatgggtc ctgagacgaa ccctgttgag gggagaaacc gttccgatgg
     2581 tgcttgagct tcccccgtac cgcatgccga ccctccgcgg agtggcggtg cacacatggg
     2641 agcggagctg ggaatacgtc aaaaaggcgg gcaccgtgat tctctccatt tccattgttc
     2701 tctgggccat gaccgccttc ccccgcaggc ctgaggctga cggcacgtac gcgcgggaaa
     2761 tcgcggccgc ccatggttca tatgaacagg gcgtgcggga actggccggg cggctggcgc
     2821 tgccggagga cacggacctg gccgcgctgc ttgaaaagcc tccgcgcgac gagtccggca
     2881 tcgcggatcc gcatctggcg cactttttca gcctcctccg gcagaaaaga gccattgagc
     2941 ggcgctttgc tccgcatcat catcgcgaag gcgaactgca cagcgatccg gcctgcctgc
     3001 agaaggagct gatgctcacg catctgcgca gcgccgatcc ggcagcctac cggctggtgg
     3061 cgcattatga cgacaaggtt ttcgctcccc gcgaacaaat cctgcagcag attgaacgaa
     3121 agcggcatgc ggacggtctg gcttattcga tgattggacg gatcgggcgg gcgctggatc
     3181 ccgtctggaa accaatgggg tttgactggc gcatcggaac cgcgctgatc ggcgcgtttg
     3241 cggccaagga agtgtttgtg gcccaaatgg gcatcatctt cgcggtgggc ggcgacgagg
     3301 acagcgccga cgccctgcgg cggcagcttc gcgcgaccta tccgccgctg gtgggcctgt
     3361 gcattctgct gttcacgctg atcagttcac cgtgcctggc caccgtggcg gccacgcgga
     3421 aggagaccgg ctcgtggggc tgggctcttt tccagatggc gtggctgacg gcgctggcct
     3481 atgccgtgac cgccgccgtc tttcagatcg gatcgctggc cggatgggga gtgtgaacca
     3541 tgcttgaaaa cattctggtg gctgtcatag tggggctggc gcttggattc atcatccgcg
     3601 gcgcgatacg ccgcgcgcgc ggaaaagaca gcctgtgcgc gggatgcccc atggcggggc
     3661 gctgcggcga tgacgcgcgc agtcaaatga aatgcggaat caggaaatga aaacatcttt
     3721 ttcagccata tggacggcgc tgcttctggc gggcgccgcg aacgtttgcg ccggcacgat
     3781 cccggagctt ctcgaccggg ccgccgcgct ggaggccgcg gccggagtgg attccggagg
     3841 atatcccgac gcggaggagg tgcttgttgc gggcatccag aaaattgcct atgaggccga
     3901 cggcacctat gtgcagtggc acgaggagta cgtcaaaatc ctgaccgagg agagccggcg
     3961 cagccacctg accctctcaa gctttttcac gattccctat cagcggggac cggaagactg
     4021 tcagatcgcc ctcgtggaga ttatccggcc cgacgggacc gtgattccgg ttgacgtgga
     4081 ggccgagagc cgcgtgatgg tgaatccgtc ctccatggag gacaacatct acaatcccaa
     4141 tgaccagctc attcaggtga acatctccga cctgcagatc ggggacgtgc tgcactatgt
     4201 tctgtttgac cgaatcgtga agccgcgtgt ggaacacacc tggagcgact ggctcgtgtt
     4261 cgaggggctg cgtccaatgc tgaagcaggt ggttgaaatt tccggcccgg cccggatgcc
     4321 cctgcagtcc atcgcgcaca agagcgaaat cccgggcacg gtcgtgttcg aggagcggac
     4381 ggatggcgac cggaagatct ggcggtggga ggcgcgggat gtgccgcgca tgttccccga
     4441 gccgggcatg cccgcgcccc acacggtgat ccagcgcgtg ctggtcagca ccatccccga
     4501 ctgggaaacc atctcccgct ggtactggaa gatctcggag ccgcacttca aggccggacc
     4561 cgcgctggag gagaaggtgc gggagctgac agacgccctg acgacccccg ccgacaggac
     4621 ctgggcgctg ttcaagtttg tttcgcagga agtccgctac atggggatca cgaaggaggc
     4681 cgaatcgccg gggtacgagc cgcacgacgt catggacacc tttgaggccc gccacggcgt
     4741 ctgccgcgac aaggccgcgc tgcttgtcgc gatgcttcgt ctcgcggggc tggacgccta
     4801 tccggcgctg attcacaacg gccccaagct ggactccgaa gtgccgcagc cttttttcaa
     4861 tcacgcggtg gtcgccgtga agaatgaaga cggcgggatc atcctgatgg atcccacgga
     4921 tgaaaccacg accgagctgc tccccgccta tctgagcgac aaaagctacc tgatcgcgtc
     4981 gcccgagggc gagccgctgc gcacatcgcc gattgtcccg gcggatcaga acatgatgac
     5041 gattatcacc accggacggg tggaccgggc cggcacgctg ttcgcggaca cggtgatgga
     5101 attccgcggc gtgaacgaca acgcctaccg ggggcttttc gcgcggcgca agcccgagga
     5161 gcggcagcag tttttcgagg gcctcgcgaa gagcatggct ccgggcgcgg cggtcggccg
     5221 cttcagcatc gagccggccg acatgacgga cacctcgacc acgctgaccg tgcgcatctc
     5281 cttcaccgca ccggatctgc tgatcggccg ggagggcacg gcggccctgc cccttccgca
     5341 ggcgggcccg cgcctcggcg tggcgaattt cattgtcggg cagaccggcc tgcagacgcg
     5401 caaatatccg ctggtgaccg gcatggcctg cggggtggat gaacgcgtga ggatcgagct
     5461 ggatccttcg cttggcgaag ccgaggccct tccccgcgga acaccgcagg actcggacac
     5521 cctcctctgg agcatggaat cgtccatgga gggccggatt gtggacgcgg cggcacgctt
     5581 cgtcctgaag ggcgtcgaat tcagccccgc ggaatatctc gacctgaaga agacgctgaa
     5641 gcagatggaa atggactacc gcaaaatgcc gatcctcggg cgggttgaag ccgggggggc
     5701 ggatgcctcg cggggcgcgg cggccgagga agagccggag gtcgaggcgg attccgaagt
     5761 gctgaacatg actctggtct atgagctggc ggacaccggc cgctggaccg agatcagcac
     5821 cgtgaaagaa cgcatcctga cctatgccgg aaagaagaac agctctgaaa tcaagatcgg
     5881 ctataacgag gcatgggaat cggtggaact ggaatatgcc cgcgtgacgg ccgccggcgg
     5941 cgcagtcagg gaaatcagca cgaatgaaat caatgtcatg gatgcgccgt gggtcggcgc
     6001 cgcgccgcgc tatccgaagg gaaaaatcct cgtgatcagc ctgccggcgg ttgaagtggg
     6061 cagcgtggtg gaataccgca tacgccggga atgccgcgac cggccgtttt tcagcgcggc
     6121 cgcgcacctg cgcgccacgc gccgcataca gcagatgacg gtccgcctct ccgcgccgga
     6181 agatctgccc ctgcacctcc tttccgagaa catctccggg gatcaggtgc gccacagctc
     6241 ccgcgcgcag ggcgggcggc agtttcacaa atggatatcc ggccccgccg agcccgtccg
     6301 gcaggaggac agcctgccgc cgtggtggac cttcaatccc tcgctcttcg cctcttcggg
     6361 gaactggtcc ggctatgcgg ccgcgctgca tgagcttttc acggccgccg ccgaaggaca
     6421 gacccagacc gtcgagcagg ccgccatcat cacggccggc acggacgacc cgcaggtgaa
     6481 gctggaaagg attcgcgatt ttgtcgcgct caacaaccgg cgcgccgggc cgggtctggg
     6541 cgtcatcccg ctgtcctcca tcacgccggc cgaccagacc ctccggtccg gctacggcaa
     6601 cagccccgac cgcgcgattc tgcactatgc cctgctcaag gcggccggct tcgaccccga
     6661 gtttgttctg gtttcgtccc tgccgacgat tgaggaggtc agccgcccct accgcgaaat
     6721 tccggcgatg catgcgttcc cggaagtcgt cgtgcggctg cgcgaccgcc tgccggatgt
     6781 ggaacccggc cggcacgtct atttcaacga cacgacacag tacgatgccg ttggcgcgag
     6841 cggaaacgag gggctgctgg ggatcacgct tccagatgcg gagttctgcg agattcttcc
     6901 cgcaaagccg acccgcatgg agaccgtcag ccgcatggat ctgcaggagg acggcagcgc
     6961 cgtcatttcg cgcaagatcc ttctctacgg aagcaacttc ggcgacaacc atcagctctt
     7021 tgccgagatg cagcccgagg agcggcggcg ctatttccgc gaactgatcg cttcggtttc
     7081 acaggcggcg gaaccggaag gcgatctttt caccgatttc ggccgttatc caggaatcgt
     7141 ggaatatgca gtctgggtgc cggactatgc cgtgatcgac gagggctttt tctactttcc
     7201 gctgccggac acgccggccc ggattctccc catgcgggcg gaccgaaggg tcaacccgtt
     7261 ctatttcccg gatgaaacgg tccggagcct gcggatggat gtcacctggc cggaaggtta
     7321 cgcggtgaag cttgcgcccg agtccatgca ggaggacggc gcggccggcc acccggttgc
     7381 gcttcgactc cagtcgggcc ccgcggcgga agaaactgcg tggcggatcg aatccaccgt
     7441 caccgtgaag cccgtcgtgg tgccggcatc ggactatgcg gcgttcctcg aactcgaccg
     7501 gaaaatggcg caccggcgca accgtttcat gcttctgcgc agggaagaat aatccgccca
     7561 aaacttcccg ccgcgcgccg catattaaat agtactattt tagtactatt ttcacgggac
     7621 gggcgaaatc cggccttttc cttcattttt tcacgcgtct tttcccgcac ggagccgccg
     7681 gtcccgccgg gccgcaaaat cacgaagaaa atgcgcttcc tccgtttcag aacctgtttt
     7741 ggggcatttt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtttttg
     7861 gccgtttttt gaccattttt gatgattttt gaccattttc tgcggttttt tcgcgtccgg
     7921 agcgttcaac cccgcgcttg acggcagggc tccgcttcat cataactgat ggggttttca
     7981 ttagaaagag gcactcgaat ggctgcaaaa catgttggac tgggccgcgg actcggcgcg
     8041 ctgatcaagg aagttcccgc cgcgccgaag gcggatgaaa aagatgctcc ggcgggaggg
     8101 atcacccgga ttccgacgga ctccatccgc aagaatccgt tccagccgcg ccacgccatg
     8161 gagcaggagc cgctggccga cctcgtgcga tccatccgcg aaaaaggcat cctgcagccg
     8221 ctgctggtga ggccggcctc cgacggctat gaactgatcg ccggtgagcg ccgcctgacc
     8281 gccgcgcgcg aggccagact cagggaagtg ccggtcatca tcatgagcat agacgaccgc
     8341 gaggccctcg aaatcgcgct gatcgaaaac ctgcagcgcg aaaacctgaa tgtcattgaa
     8401 gaagccgacg gctatcacga gctgtgcgaa aaattcggca tgacgcagga ggaagtggcg
     8461 gcccgcgtcg gcaggagccg cgcggcggtc accaacaccc tgcgcatcct cggcctgccg
     8521 gacgacgtga agcagttcct gtccaagtcg gagctttccg ccggccacgc gaaggttctg
     8581 ctgggcctgg aaatcccgca ggagcagtcg ctgctggcga agcgcgtcgt gaaggagggg
     8641 ctttctgttc gcgcgctgga aaaaatcgtc aaccgcgcca gacgcacgcc gaaaaaagcc
     8701 cgcacacggc gttcagacat tcccgaggag catctgcgct atctttccaa ccgcctgcat
     8761 cagcacttcg gcacggccgt gaacatcacg ccctgcaaaa ccctccccaa cgggaaaaaa
     8821 tccaagggca gcctggagat caatttttat tcctccgacg agctcgaccg catcctcgcg
     8881 atgatcggca tcacggaaga tttgtaatct atccttcaag cacccgctgc ttttcaaggc
     8941 cgcggacgat gatggccctg cccatgcgga cggcgggaca ggcgtgctgg cagaaaccgc
     9001 atcccctgca gatctccgga ttcacgatcg gccgcgggat gcccgtttcg gaaaaactgg
     9061 tttcaatggc gttgtagggg cagtattcat cgcacaccat gcagtgctcg ccgtcggtcc
     9121 acgcgagaca ggcttcgcgg ttcacttcgg cgatgccgat ctgaatcatg cgcttttcgt
     9181 ccacgcttgt ttttttgatg gctcccgtcg ggcaggcccg cgagcagttg tcgcagaatt
     9241 cctcgcaggc gccgaattcg ggattcatct ccggctggaa ccatgaggtc agcgggcggt
     9301 ccacccggaa acccgcgcgc agaatgcccg tgggacagga gtccacgcag gcatagcagc
     9361 ggtgacagat cgcgccaaac tgatcggcat cgtgcgcgcc cggcggcagc gcaggcatcc
     9421 atttgttctt tgtcttgaag cccggcagat gcgcggccgc acaggccgcc ggcaggccga
     9481 tgcaaagccc cgccagcagt tcacgccgtt ccttgctgaa agtttgcgcc ggtgatttcc
     9541 gcagatgcct caggaaatca aagaaatgcc ccgccggaca aaagtgcgtg caccagatca
     9601 tcggctggaa tattccgagc agcagaaaca gcggaaagac caggccggga accagcgcaa
     9661 gagcattgag ccccctgccc gtccagagca ggtcgcgctg gagcagcggc tgcggctcca
     9721 gaatgaaaag cagcgggaat cccgccagcg aactgaagat gatgatccag aagatgtatc
     9781 cgctccagcg gcggcggctg acatgggcga tgcatttgcg cggcgacgcc agatgaaaaa
     9841 gcgttccagc cggacagatc cagttgcaga acatgcggcc cctgaacaaa cccatgagca
     9901 gcacgaccag cggaggcgtc atccagaaca cacccgcata ccatccccgc tgcgcgaggg
     9961 ccgacgcgat cagcgaaagt ggcgaaaacg acgggaaaat ccgcttcatc cagaaaggca
    10021 gcggcccgcc gaacgcgaac agaaccgcca gcgccagcag gagcaggcgc acagcggcgc
    10081 gcactgcgga acggggcccg agatgcagaa tccggatcat cgcagcgctc aggcctccac
    10141 acgatgaatg ttcatcttcg cgaggtcggt ctcgccgatt ttcatgtcgc gggcaatcgt
    10201 cagatagcga acggcctcga tggaatcatg aaacagggtg ctggtgaagc tgtcggcggc
    10261 cacctggtct ttggacacga ccagcaggtt gggatatttc atgtcctttg acgggccctg
    10321 cggcccgcgg ctcagcatga tgcgggtcgc atcaatgatg gtccagtgcg gtttgatcag
    10381 cgtgcagcag tcggcgatgc actgatgcag gtcgttgcgg tgccaccagc cgcggtccgc
    10441 aacggcgccc atccagtttt tcatggccat ggtcagcgtg gcgcctccat gatgcttggc
    10501 caccggcatg ttcaccagca catcggtttc catgaactga ctggccactt tggctgtttt
    10561 cagggaacgg ccctgcggaa gctcgaagga cttccagtat ttcttttcct tttccagatt
    10621 gatcatcggg gtctttgctc tggcggcggc atcctgcaga ccgctgcgcg tgaacgcata
    10681 ggccgcatcg ttgcagggat tctccggaat gactgttttt ttcacgccgg cgtcgcggca
    10741 gccctccaga aacgccgtca ccagctcggg atgcgtgcac gcgccttcat ccggtgtgcg
    10801 cgcccaggcg gcattaacct tcagggtcag ggtgtcaggc tgcggaccga agcccccgtt
    10861 ttcgaacatg atttccagag ctttcccgat cagggcgcgc ttgtccccgc cgtgaatcac
    10921 ccagacatcg ggaccttcga ccggggaccg gtcttcagca agtgcaagcc atgacgggcc
    10981 cagcgtcagc gccgcgccgg atgcaaaggc ctgcttcaaa aacgttctgc gggtcgtctg
    11041 cctcaaatac atggctcacc tcatatgatg ctgttcccag ttagtctacc ccccgccgga
    11101 acaataataa acccgaattc ccaaaaagat attcgtccgt gctgatggcg aaaaagctgg
    11161 ttgccggagg ttcgagctga tagagtgcga tcagatcgtg ggtttgtgcg tctggacttg
    11221 gccatgaggg gaacgcatgg gacgattgaa acgaggagta tggctgctga tatgcgcctg
    11281 cgcatggggg cacgcctgtg cagacggccg ggccgccgat tcgccgttcg ccctgacggc
    11341 tgaagaacag gcatggctca cccaatatcc cgcaatccgc atcgggatca tgacggactg
    11401 gcccccgctc gacttctgcg atgtcgacgg caaaccctcg ggcatcggcg cggatttcct
    11461 gaatgaactg aaccgcaggc tcggcggcat catccaaatt gaatgcggga cgtttgacga
    11521 caatatccgg gaagtcaaaa cgggagagct tgacggcctg atggacgtca ctcccaggcc
    11581 cgaacgcgaa gagtttctta atttcacccg gccctatctg accattcccc atatcatcgt
    11641 cgggcggaaa aacggagcct actacagttc cgcttcggag ctgaaagggc gaaccgtagc
    11701 cctggagcgg gggtttggaa atgtcacatg gtttcaggag aattatcccg aagcgcagat
    11761 tcgcctctac gacagcacaa gcagcgcgct ggacgccgtc tcgcggggcg aggccgacgc
    11821 ctatgtcggc aaccgggctg tcgccctctt cctgatcgag cgcgaaatgc tgacgaacct
    11881 gcagctgcag ggacgcgccg aaaaaccgcc cgtcctgctc acgctgggcc tgcgcaagga
    11941 ctggcccctg gcgatccata tgctggaccg cgcgctggcc gagatcatac ggactgatga
    12001 acgcttcatt ctttccaaat ggttcgatgt cgcaagccgc gccgggctcc agctccagct
    12061 gaccgcagat gaacgcgcat ggctgcaggc gcacggcgag atccgcatcg ggatcaacaa
    12121 caactggccg cccatggatt ttgtggacga atcgggaaat ccgtacggca tcggcacgga
    12181 cttcatcaat ctgctgaatg aaggccttca cggccgcatc aaaattgtgc cgggtccatg
    12241 ggagcagatc taccgcgact gccttgagaa ccgcctggat gcggtgatgg acatcacccc
    12301 gcgcgaggac cggctggaat ttttcaattt caccaagccc tatgtcaaca ttccccatgt
    12361 gatcatcgcc cgccagcaca ccggttattt cgacgccttt gcaagcctga gcgggaaaac
    12421 cgtggccgtg gaaaaagcgt tctttgtcgg ccagcacatc cggaaggagc acccgggcat
    12481 cagtatcctt gagtatccat caacgcgcga ggccctgctg gccgtttccg aagggcgggc
    12541 cgacgcctat gtcggcaacc gcgccgtggc cacatggatc attgcccgtg aactgctctc
    12601 caatctcgaa atccagggga cggttgttga aacttcctcg atcaacgcca tcggcgttcg
    12661 ccgggatatg cccgaactgg ccgccatcct ggaccgcgca ctgatctcgc tcccggccaa
    12721 aagcgtgcac tcgatttacg agcgctgggg cggcgggggc gaagccgtca gcctgacatg
    12781 gatgggcctc agcacaccgg aaaaagcgtg gctgaatgag cattcggtga tccgtgtcgg
    12841 aagcagaaag cgcattgccc cgctggaata cattgacaag tttggagttc cgcagggcat
    12901 ctcctgggac tatctgagac gctttgaaaa aatgctcgga gtgaaatttg agtttgtgga
    12961 cctcggagac tggtctgagg ccgaagccgc gctgcgggcc gggcaggtgg acatgctcac
    13021 cgccacctct gattcgcaaa tttcggccga agggctggcg ttcacccagc cctatctctc
    13081 catgcagacc gcgattttca ctcatcagga cacgccctac atcgggcaga tcgcggattt
    13141 gaaggacctg cgggtggtcg cggttccggg tcattcctcc acggtcaaca ttcagcggga
    13201 agtcccggac atcacgctga tgcgtgccaa ggacatgccc gaggcgcttc ggatgctgga
    13261 gaaccggaag gccgacgcct ttgtgggaac gctgctggtc acaagccatt atattcagga
    13321 gggaggccat acccgaatca aggtctccgg agaaagcgcc ttcgtcttcc atcccgcctt
    13381 tgtgtgccgg caggactggc gcgtcctcac cgacatcctg agcataattc tcgcggacat
    13441 tgacgtgagc gagcagaacg ccattctgcg caaatggacc accgtgacct atgaacagcg
    13501 catcaactat tccctgctct accgcatcgt cggatccgtc cttgttctgc tggccgtgtt
    13561 cctctactgg aaccggcggc tggccgccga gatccggcgc agacggcagg tggaggaagt
    13621 gctccgccgg agcgaggtgc agctgatcga ggccaaggag cgggccgagg cggccgacca
    13681 gatcaaatcc gccttcctgg ccaacatgtc ccatgagctg cgcaccccgc tcaactccat
    13741 catcgggttc accgggatca tcctccagga gctggccggc ccgctgaaca gcgagcagaa
    13801 aaaacagctc gagatggtca gaaactccag ccagcatctg cttgcgctta tcaacgacgt
    13861 gctcgacatt tcaaaaatcg aagcgggcca gatggacatc accaccgaac cgttcctgat
    13921 gccggatgcc gttcgggcgg cggtgcagac agtgctcccg ctcgcccgga aaaagaacct
    13981 cccgctgcac gtggacatag cccgggacgt gggcgaaatc gtcagtgacc ggcgccgggt
    14041 ggagcagatc ctgatcaacc tgatcaacaa tgcggtgaaa ttcacagaca agggcagcgt
    14101 caccgtgtcc tgccgcatgg agaacggcca gatccgaata tccgtgcgcg acacgggcat
    14161 gggcattcgg ccggaaaatc agggcaagct tttcaaggca tttcagcaga tcgactcagg
    14221 aaccacgcgc cagtacgaag gcacgggact cggactttcc atttgcagaa agcttcttga
    14281 aatgatgggc gggacgattg cggtcgagag cgaatacagc cggggcagca ctttcagctt
    14341 tacgctgccg gtgaataagg agaagatgaa cccatgaata ttctggtaat cgaggataac
    14401 gaccagaaca tgtacctgac agagtttctc ctcagccggc acggacactc cgtactcaag
    14461 gccagcaacg gcgccgacgg catcgctctt gcccggcaga gccgcccgga cgcgatcctc
    14521 ctcgacatcc agctccccgg catggacgga tatgagattg ccggagttct gaaaaatgat
    14581 gaggtcaccc gccacattcc agtgattgcc gtgacatcct acgccatgcg cggcgaccgc
    14641 gagaaagtgc tcgccgccgg atgtgaaggc tacattgaga aacccatcaa tcccgacacc
    14701 tttatgaagc agattctgtc cttcattccg ggaggtcaaa aatgaaaacc atcctcattg
    14761 cggacgacat agaggccaac cgctacctcc tgcagtcgct cctgaagggc agcggctaca
    14821 aagtgtccac cacccaaaac ggccgcgagg ctcttgatgc cgccaggaaa aatccgcccg
    14881 acatgatcat taccgacatc ctgatgcccg agatggacgg cttccagctc tgccgggcct
    14941 ggcgcaccga caaggcgctc gccggcattc ccttcatttt ctacactgca acctataccg
    15001 acccgtccga cgagaaattc gccctcgatc tcggagccga ccggttcatc acaaagccgc
    15061 aatcacccga aacgctgctg caggcgatcc aggccgtgtt tgaggagacc gaaacaaaag
    15121 gcgtccacgc cgccgccagc ggcgtctcca cgggagacca gtacagccag gtgctcttca
    15181 agaagctcga gcagaaagtg caggatctcg aggaaacccg cggcaagctg gaaaaggaaa
    15241 tccgggaccg gcagcaggcc gaggatgagc gcatccagct cgagcggcag ctccggcagg
    15301 ccaagaaaat ggaagcgctg ggcgctcttt ccagcggtgt ggcgcatgac ttcaacaata
    15361 tcctgcaggg catcaccgga tatgccgaac tggccctcga cgcgcttccc gacgcccccc
    15421 caggaggccc gcgattatct cggggaaatc atccgcgccg gaggacgcgc ctcggagctg
    15481 atccgccaga tcatgacgtt cagccgggaa gtcaaggtcg gccggactcc gtgtctgctg
    15541 gaagccgtcg tccgtgaagc catcaacctc atgcggggga agatgcctcc gcgcgtggtc
    15601 atggatgcgg atattatcag cggcggcccc catgcgctgg tggatgccgg tcaaatccac
    15661 cagatcatca caaatctcat ttccaatgcc ttcgatgcca tgcgcgaagg aggaggacac
    15721 gtccaggtgt cctattatga ggaggacgtg gatgagcaca aagccgtgca ggctccgcgc
    15781 ctgaagcccg gccgctatgc cgtgctgcag gtcgccgaca ccggccccgg gatggacccc
    15841 gccacgctcg agcgcatttt cgagccctat ttcaccacca aggccgcgca tggcggcacc
    15901 ggactggggc tggctgttgt ccatggcatt ctcgagaccc acggcgcctc tatcagcgtg
    15961 gaaagcaggc ccggactggg cacaaccttc accatctatt tccccgcctg ccagctgcgc
    16021 gacaacggcc gtgatgtcgg gccggaactc cgcggcagtg aaagcattct gctggtatgc
    16081 cgcctgaatg aacagtcaaa cggaaatccc ggaatggcgc tgggcgggct gggttatcag
    16141 gtccgcccct gctcgggcga ggccgctctc caggaagtcc gaagcgaaca gtccgggccg
    16201 gccgccgttc tcattgactg ctgcactccg ggcattaatc cggcttcgct gaccgcgcag
    16261 ttcaagcaga tggccccgga actccccgtg gtgatgtatt acagcgcccc ggtcaaaccc
    16321 ggggaggaga gcgacattcc gctgggaatt catttcgtgg cggaccagcc ggcctcgctg
    16381 aaggacctgg ctgcagagat ccgcgccctg ctggcatgag accctaaaac gcgcgaagcc
    16441 cgcgcctgcg cttgacttaa caggaaaaat ccgttcatct ctttatcatt ttcggggggc
    16501 cgagaggccg cagccagaga ggaaatatat gaaggtggta aaattcggcg gatcttccgt
    16561 cgccagcgca gaacagattt caaaggtcat ccggattgtg acctccgatc cggaccgccg
    16621 cattgtcgtg gtctccgcgc ccggcaagcg gagcaaaacc gacaccaagg tcacggacct
    16681 cctcattgcg ctggccggca gcgtgatcgc aaataaaaat cacgaagccg atctcaaggc
    16741 cgttgtggac cgctatgccg agatccagcg tgacctgcag ctgtctccgc aggttgtcga
    16801 aaccatcgaa tatgatttgc gcggacgaat tgcgaagcgc ggaaaaaacg atgcccagtt
    16861 catggacgcc atgaaggccg ccggcgaaga ctgcagcgcg cgcgtgattg ccgacgcatt
    16921 cacaaaagcg ggcttcccgg cgaaatacgt gaaccccggc gacgcgggca tgctcctcag
    16981 cgacgacttc ggcaatgcgc aggtgctaga tgagtcctat ccgaaactgg cgcagcttaa
    17041 ggacgtcaag gaaatcgtga tcttcccggg cttcttcggc gtgacaaaaa aaggcgatgt
    17101 ggccaccttc ccgcgcggcg gatcggacat caccggagcg attctggcgg cggcggtaaa
    17161 agccgatgtg tatgaaaact tcaccgatgt ggactccgtc tgcgcaatgg atccgcgcgt
    17221 gattgaaaat ccgcccgcga ttgatgtcat gacctatcgc gagatgcgcg agctttccta
    17281 tgcgggattc ggcgtttttc acgacgaggc catcgttccg gccgtgcacg ctgaaatccc
    17341 gatatgcatc aagaacacca ataatccgtc agcacccgga acgatggtgg ttccgacgcg
    17401 cgcgcccaac gagcgcaccg tcgtcggcat atccagcgcc cccggcttct gcgccatcta
    17461 catcgacaag tatatgatga accgcgaaat cgggtttggc cgccgcctgc tgcagattct
    17521 ggaggatgaa aaaatatcct ttgagcatac gccttccgga attgacagca tttccgtgat
    17581 tctggcctcc gccaacattc ctcccgcgaa ggaggagcgc gtggttggcc gcatcaaggc
    17641 cgaactcaag cccgatgcgg tcgtggtgga acacggactg gcgctgatca tggtcgtggg
    17701 cgaaggcatg cactatgccg taggcatggc cgcgaaggcc acaagggccc ttgcggatgc
    17761 gggcgccaac atcgagatga tcaatcaggg cgcttcggaa atcagcatga tgttcgccgt
    17821 gaaggacacc gaccgcgaaa aagccctgcg ctcgctgggc cgcgcttact tcggaatctg
    17881 aaaagttcca atcattggaa gtttttcggc gcaaagttcc agggattgga acttttcact
    17941 cccggcgcgc aatcatgccc atgaagttgt aggccaggcg cgcggccgtg aactcggaga
    18001 tgttcgaacc cgccatcggg gacacctcca cgacatcgaa gccgatcact tcgcaccgcc
    18061 gggcaatgtg attcaggatt ttcagcgcgg gataccactg catcccgccc ggctccggcg
    18121 tgcccgttcc gggcatgatg gagggatcca gagcgtccac atcgaaggtg acataaacct
    18181 tttccggaaa gttatcgagc acaaggctca tgtccaggcc cttctcgacg gtttcctcca
    18241 tgtcatagtg catgatggca ttttcaaccc gaatctccag atcctcgaga gacaggctcc
    18301 gcacgccaat ctggcacagc gggataccca tctccaccgc gcggcgcatc acacaggcgt
    18361 ggctccattt ggtgccttca tattcgctgc gcaggtcggc gtgagcatca aactggatga
    18421 tcccgaattt ctcgccggca tcatgcaggg ctttgatggc gccatacgtc acggtgtgct
    18481 cgccgcccag catcacggga atgtgcccgg catgatgaat tttgccaatc tgctccgcga
    18541 tctccgccag cacggtttcc tcgggcaggt cgcagtccac gggcaaatgc gtgtaaatgc
    18601 cctcctcggc gggaatgctg tggccgtcga acagttcaag ctgctgcgaa gcattcagga
    18661 tggccgacgg cccgcgcacg gttccctcgc cgtacgatgt gcttttctcg tacggcacag
    18721 gcagaatgtg gaacttcgcg ttctcgggca gcggcggatc catgtccgat tcaagaaacg
    18781 acggataggt cagttcattc atctgcttct ccttcaggaa agcctgcttt tgaaatcctc
    18841 gtagccgaat ttgcgcacca gttccggctc cgcgttttcg gatgcgcaca gatagatgga
    18901 cggcagcggc accccgttga aggtggtggt tttgaccatg gtgtagtgtg ccatatcctc
    18961 gaacaccagc cggtcgccgg gctgcagcgg tttctcaaac gaatagtccc cgatcacatc
    19021 gcccgcgaga caggacgggc cgccaagacg aaagttgtaa tcgaaatgat ccggcttttc
    19081 cgcgccgcgg atggccgggc ggtacggcat ttcgagcaca tcaggcatgt gcgtcgtggc
    19141 cgaggtgtcc agaatggcaa tgtgcttctc gttgcgcttg atgtccatca ccgtggaaac
    19201 cagcacaccg gcgttgagcg cgatcgcttc gccgggttcc agataaacct gtacgccgta
    19261 tttttcccgg aagcgcgtga tcagatcgca cagcagatcc acatcgtagt catcgcgcgt
    19321 gatgtgatgt ccgccgccga aattgatcca cttcattttc ggaatgtatt tttcaaaccg
    19381 gtcctcgaat gaaaccagcg tgcggaccag cgcatcggca ttctgctcgc agaggacatg
    19441 aaggtgcagg ccggatattc cggtgaggtc ctgctcctca aactcggtga aacggatgcc
    19501 aaggcgtgaa tagcgggcgc agggatcgta catctcgacc ttcgcctcgg aatgctcggg
    19561 gttcacgcgc aggccggcgg agatatcgcg tccggacttc gcaatgatat cgcgcgcaag
    19621 atgccactgg ctgaaggagt tgaaaaccag atggtctgcg attttgacga gttccgccag
    19681 atccgccgcg gaataggccg gagaaaacac atgcacctcg ccgccgaatt cctcgcgccc
    19741 gagccgggcc tcgtgcaggc cgctggcgca gacgccgcga agcgtctggc gaatcagcgg
    19801 gaaaacgcgg aacatggcaa accccttcag cgccatcagg atcgtgcatc ccgtgcgctt
    19861 ttgaacagta tccagcacgg ccaggttggc cttgagcttg tcctcaaaga ccacgaaaca
    19921 gggagtctca acgcttttta gccacggaga attcaccgca aaagatcgca aagagcgcaa
    19981 agattccttc attgagatgc tccctgcgtt cgaatgtatt tcttgatctc gaatttatag
    20041 gacccgaagt taatcagcaa cccatgttct attccggtag ccttgaggta atgcagcacc
    20101 tgcgtttcat gttcaatggc cattgatttt gcagccttca gttcaaccag caaacagtca
    20161 ttaacaataa tatccgcaaa gtattcgcca atgaccgtct catcttcgtc aagaatggta
    20221 atgggttgct gctgtttaat gctcaacccc tctttgcgca gtcgagacac aagcgcattc
    20281 tcataaacct tctccaggta cccgttccca tgataaacgt ggatggcgta tgcaatctcc
    20341 cgcacctgat cgcataatct ctttatttca tccatccctt aattctttct ttgcgatcct
    20401 tgtgatcttt tgtggtgaat attattttaa ttccaccgcg gtccacggca ggccgtgctt
    20461 gttgagcttg tccatgaacg ggtcggggtc gagttgctcc atgttgaaca cgcccctgcc
    20521 cttccacgag cccgtgagca tcagcatcgc cccgatcatc gcgggcacgc cggtcgtgta
    20581 ggaaatggcc tgcgactgca cttcgcggta gcaggcctca tgatcgcaga tgttgtagac
    20641 ataaagcttc ttgggcttgc cgttcttcac accggtcatc aggtcgccga tgcaggtgcg
    20701 gcctttggtc agcggcccca gcgaggccgg atccggaagc agcgccttca ggaacttcag
    20761 cggaacgatg tcgtgcccct cgaacttcac gggatcaatg cgggtcatgc cgacgttctg
    20821 cagcacttca aggtgcttca gatagttgtc ggagaaggtc atccagaaac gcgcgcgctt
    20881 caggccgcgg atgttcttcg ccagtgattc cagctcctcg tgatagagca gatagatttt
    20941 catcggtccg atgccctcgg ggaattcgaa ggtttcggaa acggaaagcg gatcggtgga
    21001 aatccactcg cccttttccc agtagcgccc gcgcgccgtg acttcgcgga tgttgatttc
    21061 gggattgaag ttggtggcaa agggctggcc gtggctgccc gcgttggcgt ctatgatgtc
    21121 caggtcgtgg atttcatcga agtgatgctt ctgcgcccac gcggcaaaca cattcgtgac
    21181 gccgggatca aagccgctgc ccagcagcgc catcaggccg gccttcttga aacgctcctg
    21241 gtattcccac tgccacttgt agcagaactt cgcctcatcc ggcggctcgt agttggccgt
    21301 gtccagatag ttgacgcccg tctcgaggca ggcgttcatg atatgcagat cctgatacgg
    21361 cagggcgaca ttgatgacca tttcggcgcc ggttctgcgg atcaggtccg ccatttcctt
    21421 ttcattatcg gcatccacct gcgcggtgtg gatcggccgg gaaatctgcg cggcaatcgc
    21481 gtcacacttc gacttcgtgc ggctggccag cgtgatgtcc gtgaaaacct ccggcacctg
    21541 cgcgcacttg tgcgcgacca ccgccccgac gccgcccgcg ccaacaatca gaacctttgc
    21601 cataatacgc ctcccaaaat gcaataaccg ttcaaccgtt ctgacacttt aggctatttg
    21661 caatacaaag cgcaagaccg cagaaatcct gagaacttcc aggcattgga gcttttcggc
    21721 atccggggcc ggcatggtat attcgccgaa agacccgtaa tcgaatgttt attgttcaga
    21781 cccttcttcc aaagcgggca tgccgcgggg gccggacgca gcggcaaatt tataggccat
    21841 aaagagcgcg atgattgatt tttggggtgt tttgatgcgc tttttgcagc gagcatgaat
    21901 atagtactat tttagtacta tatgatcttt ttctctgttt tagggcattt tttagtgttt
    21961 ttgagcgttt tttaggcgtt tttgggttct tcgtcaatta tcgtggaaag agatcgagct
    22021 ttccgcagtg aaaaagg
//
LOCUS       DAOQWR010000026        21284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2840_length_21284_cov_5.490508, whole genome shotgun sequence.
ACCESSION   DAOQWR010000026 DAOQWR010000000
VERSION     DAOQWR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_21284_cov_5.490508"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKK36_03585"
     CDS             complement(<1..384)
                     /locus_tag="PKK36_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR93673.1"
                     /translation="MSNLSHIRNFCIIAHIDHGKSTLADRMLDITGTIQKRLQRDQLL
                     DAMDLERERGITIKSHPVAMNYRAADGKQYLFHLIDTPGHVDFTYEVERSLAACEGAL
                     LVVDAAQGVEAQTVANSHLAVNQGLT"
     gene            complement(381..1055)
                     /gene="rpe"
                     /locus_tag="PKK36_03590"
     CDS             complement(381..1055)
                     /gene="rpe"
                     /locus_tag="PKK36_03590"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNR93674.1"
                     /translation="MQKIEIFPSILACDMGHLADECRRSVEAGSDGLHVDIMDGIFVP
                     NISMGFDIIRVVDRIVDIPLSVHLMIVKPDPYVEKCVELGADTVLIHIESEGDIRGAL
                     RKIRAKGARAGITINPETPASAIDGVLDEIDEILIMSVHPGFGGQSFIAEVLNKVKIL
                     RRRLPGMDISIDGGIDTETAGRSAACGANIFLAGSSLYRANDMAAEIRAMRDNATKHF
                     NTGLNS"
     gene            complement(1113..1871)
                     /gene="truA"
                     /locus_tag="PKK36_03595"
     CDS             complement(1113..1871)
                     /gene="truA"
                     /locus_tag="PKK36_03595"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNR93675.1"
                     /translation="MTRYRLIIAYDGTAYAGWQIQPDGRTIQGELENAIHRISGQEKV
                     RVQCSGRTDTGVHARAQAAHVDLDHPMPVRKLLEGCNALLDEDIRVLKASRVSPEFHA
                     RFSAASKEYRYFIWNGNVVPPHVRNQRTWVRRPLDVQAMKKAAAILQGEHDFAAFTAN
                     PNREVEGTVRNLSVLKISKKGHEVIITARAGGFLYKMVRSLAGFLIRVGNGDLPPEAA
                     GEILRSKKRTAAVPTAAPQGLFLWRVFYGQGGES"
     gene            complement(1868..2311)
                     /gene="ruvX"
                     /locus_tag="PKK36_03600"
     CDS             complement(1868..2311)
                     /gene="ruvX"
                     /locus_tag="PKK36_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018884728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNR93676.1"
                     /translation="MSRVLGIDYGDRRIGIALSDPMRIIASPLCVIDNTGLRHALAEI
                     LALCEKHDVSQIVMGLPLNMNGTSGEAAQKVNRFMEELRRRTKIPVLTWDERLSTVSA
                     QHALIEAGTRRENRKGLVDKVAAQIILQHWLDAQPSDPFDEEPRA"
     gene            complement(2311..3402)
                     /locus_tag="PKK36_03605"
     CDS             complement(2311..3402)
                     /locus_tag="PKK36_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR93677.1"
                     /translation="MNRSNFYAVILAGGKGERFWPLSTSRVPKQFLSLVGEKPLLAQA
                     IDRLEGLIAPDNVFVLTNADLIGPARESAPGLPAENIVGEPVGRDTAAAIALGAALVK
                     KRDPNAAFAVLTADHVMGDLDVYRQTLADACSLALEEDVLITIGIRPSEPSTAYGYIE
                     AGEEFRKSSAGTRFLKAERFVEKPDAATAAEYVSSGKFAWNSGMFIWSVKAIEKALRI
                     HRLPLYQMMQNLAPAIGTGAFKDALADAFGALEKISIDYAVMEKADNVLMAEGSFAWD
                     DVGSWPALENHFPKDDRGNTVIGQMESVGAENSIVFSRGRLTALVGVENLIVVQAEGA
                     TLICARDKAQDIKQLVQKLRDSGKYGPLL"
     gene            complement(3399..4214)
                     /gene="trpA"
                     /locus_tag="PKK36_03610"
     CDS             complement(3399..4214)
                     /gene="trpA"
                     /locus_tag="PKK36_03610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNR93678.1"
                     /translation="MKFKTRIDRKFAELKKNGETGFVAYITAGDPDLAGTEEIILRLA
                     DAGADFIELGVPFSDPMADGRANQDAAFRALQAGATLDGVLACVERVRENTDIPLIFY
                     SYLNPLKAPGFDQMMRRSKTAGIDGVLLVDLSLEESAPYRKILDRNELNNIMLVTPTT
                     RPERIAKIAAYCTGFVYCVSRAGVTGQQKTVASGAAELIMQTKKSTDLPVALGFGISE
                     PRHAREMAHFADAIVVGSAIVDRFNRESHDARGRARAAAFVKELVRAAKEIRK"
     gene            complement(4211..4981)
                     /locus_tag="PKK36_03615"
     CDS             complement(4211..4981)
                     /locus_tag="PKK36_03615"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNR93679.1"
                     /translation="MTKPILTIDIGNTSVAVGLYENGEILRQTWLRHRDATAELLDAR
                     LMESADGITPCGAAMASVVPDANDTWRGAVERLFGFQPMRVTCESSLGIPVTYPRPET
                     IGADRLANAAGAAVRYGLPVIVADFGTALTFDIVSAVNGYIGGVIAPGLPLMFDYLAE
                     KTALLPHIKPEPAQTIIGKSTEDAIRSGARWGYLGMVREILRRVEEEMGEANVTVCAT
                     GGFAPWVLDGMDREIIFDHSLTLFGIGHIYELNQAKQT"
     gene            complement(4989..5699)
                     /locus_tag="PKK36_03620"
     CDS             complement(4989..5699)
                     /locus_tag="PKK36_03620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNR93680.1"
                     /translation="MPENGPVIHASVIAEPLPEWREAISARVRTRLIGRKILAYRSIS
                     STSEHLKKMGAHGAPEGFVIIAREQTQGRGQFDRKWISEAEKGLYMSILFRPPWFASE
                     TGHLSVLCAVSVAQVLDAAGLKNVTIKAPNDVLVSGRKIAGILIEPRLGDEYVEFAVA
                     GIGLNLKQTEEDWSSLELKTPATSCRMEGIELSEMDASVQLLECLDRNYAAALEGRRD
                     ALVKEWIARGGSAEIPNV"
     gene            complement(5692..6558)
                     /gene="nadC"
                     /locus_tag="PKK36_03625"
     CDS             complement(5692..6558)
                     /gene="nadC"
                     /locus_tag="PKK36_03625"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNR93681.1"
                     /translation="MKMTVETQAAADRLIALALREDLGRAGDVTSKSLISPTEKGRAV
                     ILARTPCMISGGPVAAAVFRKVDPSLKTQTVLPDGARAGAGATVLEVRGSLRSILSAE
                     RTALNFMQRMSGVATLTSQFVRRARKVMILDTRKTTPGFRLLEKYAVQCGGGCNHRMG
                     LYDRILIKDNHRNLWRKRGGDNLCGAVNAARKKYPGLAVEIEVESEADLRQVLAAKPE
                     WILLDNMPPAAMKRCVRICGGQCRLEASGGITLSNISAVAASGVDAVSLGCLTHSAPT
                     ADLSLELEWPDA"
     gene            complement(6565..8607)
                     /gene="uvrB"
                     /locus_tag="PKK36_03630"
     CDS             complement(6565..8607)
                     /gene="uvrB"
                     /locus_tag="PKK36_03630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNR93682.1"
                     /translation="MSQKAVYQIESRYSPTGDQPEAIEQICRRLEQGSRFITLEGVTG
                     SGKTFTMSNVIARWGKPALVISHNKTLAAQLYAEFKSFFPNNAVEYFVSYYDYYQPEA
                     YIPQTDTFIEKDAAINAEIERLRLAATDSLINRDDVIIVASVSCIYGLGSPEDYRSML
                     VHLKRGESADRDEVLRRLVDIQYSRNDYENSPGTFSVRGDVVDILPSYSKKGIRVEFF
                     GDEIEAIRRIDPLTGNSEASLDEISISPAKHFVMPLSKVEAAIGNILEEMRERVAWFE
                     SQNKLLEAQRIKMRTEYDMEMLKELGYCQGIENYSRHLSGREAGSRPDTLLDYFRGDF
                     LTIIDESHVTLPQIRGMYNGDQARKRTLVEHGFRLPSALDNRPMNFDEFMKAAGQTIF
                     TSATPGPFEREQSAEMVQQLIRPTGLLDPPVEVRPLANQIDDLMEEVRLRATRHERTL
                     VTTLTKLTAEDLAAYLQETGLKVQYLHSEIDAIERVEILRQLRKKEFDCLIGINLLRE
                     GLDLPEVSLVAILDADKEGFLRSETSLIQTAGRAARHVNGQVIMYADNITDSMRRMID
                     VTRRRREIQLAYNAEHNITPQGIQKSIQESLVTREEAAEVEKSVIRESGVDYNTAEVI
                     ADIEREMLEAAAALEFERAAFLRDQLAELKAPVIRERSGKGGGLNYSRPRRRKKRG"
     gene            8715..10652
                     /locus_tag="PKK36_03635"
     CDS             8715..10652
                     /locus_tag="PKK36_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93683.1"
                     /translation="MITIFQSVAHRLFGPSGALLDARVLCEENLCETCSLTASFLLAL
                     GGDRDALLRLQRAEGSNEYAGFLRRHLGTVEEEIRRACESPDFRKRLQALDKNPSDTS
                     ALRMVFFPEGAGLPETREEKIEALRQRRTIRITSLNPSPLSDPVRQMLFTSNVLLTVP
                     LSGSSLDEINCTDELKARIRSVAGEEQMYWYDHPIPVGVRPEANEILYGLRGLDTAAE
                     FERARGLMGPGKIACALSVSVTHRGLQDIARQYIESEIRSAGGFRNVDVYVFTEADTR
                     RLIREVLVPAGGDEGSLKIFGVDGEYGRHYSFLKAVAALWQAAADPGVQATFKIDLDQ
                     VFPQKELVEQTGKSAFEHFKSPLWGAHGQDADGFPVELGMIAGALVNERDIDRGIFTP
                     DVSFPDGPAAPDERIFFSRMTMALSTRAEMMTRYRPDGIDGKTACIQRIHVTGGTNGI
                     RIDSLRKHRPFTPSFIGRAEDQSYLLSVLLEEGEKLRYVHKDGLIMRHDKEAFAGDAI
                     RAASFGNIIGDYIRTLYFSEYARVLDPRGLERIKSMVDPFTGCFITPIPVIVVMLRFC
                     LKAAGYFLAGDGKTGTEFVEANHNRLERAMAFAREELKQQYEREREGWNRYYELLDRL
                     QHEPSLKKRAGQIIQDCRVRT"
     gene            complement(10722..11543)
                     /locus_tag="PKK36_03640"
     CDS             complement(10722..11543)
                     /locus_tag="PKK36_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93684.1"
                     /translation="MLKNLMICLLAVSAQADFQEVGPDQMDSGSLSLLGREVLQMPGI
                     RWQHAQTERFVYHYERKTFALKAARMAEFLFDHIAKELNAPAQGPAVRSHIFIFRSPK
                     DWQEFIRASGMKEEWAFSVVRGPEMFLQQADGTAESGSVLAHEMTHLVINRLFPRRLP
                     LWLNEGMAEWYEEFGYAAFKGIKKSRRAHFKKRDVLYPLADLFAATQYPAEVEAFYRT
                     SKMLFGWLMLRQPAEKIMPFTLDVMNGMSAEEAFRIHYEMDSADQLQKELEAFLN"
     gene            complement(11537..12994)
                     /locus_tag="PKK36_03645"
     CDS             complement(11537..12994)
                     /locus_tag="PKK36_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNR93685.1"
                     /translation="MPEIHSDIGPDYLRDESRKTGTADSISFPLNEAEIADAVRWAAA
                     NGQCITTQGARTGLTGGAVPSGGHILNLSRMKTITDRDDLIVQPGVTLDEIRAHLKSR
                     GNVFFAPDPTETTATIGGMASCNASGARSFRYGATRESILAARLVLADGDVIALCRGQ
                     CMATGRNFRLKTESGRVIEGDLPSYTTPAIKTASGYFVRDSMDLLDLFIGSEGTLGIF
                     SELHLKLQPWPASVWGVMAFFGKEPDALAFVHAVRGLKDKPASIEFFDSCSLNLLRSH
                     RLEGAPEVPADYHTAVYVEYHAADEDEASGMVERMCEALDGCGGSEDRTWIATDDHER
                     ERIRLFRHALPEAVNSLIDERRKTDPAITKLGTDMAVPDDRLDELMALYHASLERLGL
                     EYVIFGHIGSNHVHVNILPRTVKQFEQGKAQYLEWADAVIRMGGTVSAEHGIGKLKTP
                     FLLKMMGARGIEDMRRLKRLFDPQCRLNPGNLFAC"
     gene            complement(12996..14069)
                     /locus_tag="PKK36_03650"
     CDS             complement(12996..14069)
                     /locus_tag="PKK36_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNR93686.1"
                     /translation="MERLTQMMNWTLAGNSIWRIASFFVVVLAALLTGRILRHVLLKR
                     SEVMAAREKTAVSALLRAIARGMVFLLFLFGFRMGLSFLVYTEQFQQLISTTVSVLTT
                     CAVGFIVYCLTDAVQVGLARATARSSSRMNEILAPMVTKSLRITVVVLTLIQVAQILS
                     DKPITSILAGLGVGSLAVALAAKDSLQHFFGSLVILADKPFELGERIVFDGIDGNVES
                     VGFRSTKIRLLNGHLVIVPNGELSMKSITNISRRPFLKRELNIGLVYSTPPDKMEKAV
                     QILKDILDNHEYMDPKFPPLVFFTDFNDFSLGIKVIYWYGKNDWLGYMAFSHKINMEI
                     LRKFNDESLEFAFPTQTIHMADK"
     gene            complement(14222..18736)
                     /locus_tag="PKK36_03655"
     CDS             complement(14222..18736)
                     /locus_tag="PKK36_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93687.1"
                     /translation="MKKRALRHVVLTAAFCAVQGLFAETPRGDINGWGTNWMTADTDF
                     GSIYKVTITSTATRATSGLKFDQSGNWNPQWGYKAAYSTNAAVNSTIGQARINDGGDP
                     SDLSFSETSGWRYTFNLKGTYEWWDRPYVIMGTEGSPVSVLAVSDNHDTAATNAVTVT
                     IQLSAAKSAQENVWVRYTKNAWTNSYLTAQAAGSSTNYTAAIPASPTGSVIEYYVLTS
                     TMPSNIITADFDLCTLRGKKSGATNYQYSVGTPPANLGNCWHIPTNTEPFTATMRNPA
                     GDPSTNQAVYIYNGSQYQGAGNPGDQTGGWLFHKKTDAGSWSSNSLSFDSVDSNNKYW
                     VGLIPAGTYAASDEVQYYLLITYNDHDDTWIGTTNSGASSLTFADAGDAQGNPFTFTY
                     SSAAGQSPEFVWHADNRVVSGTSAQFWAKIGYAQGIGTNRWADYAAVYYTTNGTEPHG
                     HYGAAGNASTKVVAMSFDHMEEDSYPDADAMWWQGTATGLPSLTTIKYKIGAWKTTND
                     TERYADYGTAGTASNTFSFAIGTAGAQQLTVGTLNADYTTTKFFIDEVNGDTEQIVVT
                     YVPGALNLDKVEIFSNLDRRDYADADYNTDGVPDGIRPPSGDLITTNDTGAYFRAYPM
                     TPVGGGAYVWTGTVSRCGAYRLTARYSTNGMAAGSWHWYSSAGRRDHAVVVSPKKVLE
                     MTMYELNPLTVEATYNDEAGRSTFRDLLGSSEGDTDGFDPFNLEYLNYIQANCLWFQP
                     IHPSAETFRGDPSEYEPGSPYATRDYYAVSPYLGADGTETGALAEFTNFVAACDAYTG
                     SVGTVNVMLDGVFNHTAWDAVMGQGGLDLGFCTNKNDHIGWFKPGWYALWTDYGEPAT
                     YYHSVYSNDFATAPDRGDFGKWADVAEFYFGKYSALVRHNPDNNGDYLNEDDVYDYTG
                     MSADTIDLWKYFGYYTEYWLKKTGHSGTNTFVRADDDRGIDGLRCDFGQGLPPQTWEY
                     IINRTRSMKWNFIFMAETLDGGKPGYRSNRHFDVLNENLVFQFTQAQINDSWSVRSAL
                     EDRRNAYNGGAVLLNLTSHDEVLPDNDCWLVASRYGAVSTVDGIPMIFYGQEQGIQNY
                     NADPSYWYYDGFRTDHEENFGKFIPHFKQWNQLMVWSNPPPNAEGLAQWYGRVNWARL
                     NSPALQSQNRYFLSKVGGGEEGRILAVAKYEEPYASPNDKDVVLAFALMFRHGEAHTG
                     ASATYDLTGAWDLLGLDTGTLYQVRNLASSDASAEVWGSPISGASLYNDGIFVQLNGG
                     TAGGSITNDGELVQYLKLVEVDENHAPVISLPGPHILPVGAYTNFEVTASDPDSDPVT
                     LTNTVAPAGATYVGTTFSWTAGASDAGSTSLVVFVANDGQGEASSVVTNSTVIVVPAD
                     WDADGIGDDWEWNRFSSLTNNPADDADGDGKSNHYEYITDTDPLDDGSFYQNTITSAV
                     GRTVLNITAGPPTSGDRVYDVVWSTNLTESMVNWTRLNLNLPGTGGAVVLSVTNNGSA
                     GYYRTMVTLP"
     gene            complement(18899..19213)
                     /locus_tag="PKK36_03660"
     CDS             complement(18899..19213)
                     /locus_tag="PKK36_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93688.1"
                     /translation="MASCTRLPDLLLYTKNYFHATIAPAAIPCKKLKKTRKTAPKRPK
                     NAKNDQKSAQKRQKRPKILEFSNFHENISPAALAAAKNEKKGEMKGARAGKCKTAPCG
                     SK"
     gene            19204..>21284
                     /locus_tag="PKK36_03665"
     CDS             19204..>21284
                     /locus_tag="PKK36_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNR93689.1"
                     /translation="MMPSGKTAEGPRRKNLDGLFKPRSVAMVGASADPHKIGHQILRN
                     IVEGGFAGEVYPVNACAEAVLGLKTFPSVAAIPHPVDFAVISIPATAVAACMEECGQK
                     GVQAVAVITSGFAEAGNIEEEHKLKAIADRYGMALVGPNILGVVYSPSRLNATFGPRD
                     MASGGTAFVSQSGALAIGLMGWAVMEKMGMSALVSIGNKADVDERDLIEYFNHDEHVK
                     VTLVYMEGLKNGRDFMQTRISKPVVMLKVGRTQRGARAAASHTGSLAGSDQVFDAAFR
                     QLGMLRADTFREAFNWARALALPPMPGRKVLIITNGGGIGVRATDECEKAGLHLLDDP
                     EWLEETFRPVMPSYGSTKNPIDLTGGAAADKYSQAFKSAVISDKFDALLVLYCETVIT
                     DPLAIAQGVSREYEAAGRRKPVVAAMVGGERSRQAIHFLNQQGVPAFTSVADAASSLN
                     ALAQWHEISSREKDDPECSQAPREAVRIVEQARKEGRSALLEHEARQVLELCGVPVPP
                     AAFARDLDEAKLKAAALYPLAMKIASPQIIHKSDAGGVAVNIRDESELAEKYSAMMKT
                     VSERVPDAQIEGVNLVQMAEGIECAAGLSSDPQFGPVVMFGLGGVLVEALKDVAFRIV
                     PFGRVEAERLIGDIKARNILDGFRGARADRPSLVQILCAIQKLAGLVKEVDINPIRSS
                     EKGSFAVDARI"
BASE COUNT         4188 a         6388 c         6190 g         4518 t
ORIGIN      
        1 cgtcagtccc tgattcacgg ccaggtggct gttggcaacg gtttgggcct ccaccccctg
       61 ggcggcgtcc accaccagca gcgcgccctc gcatgccgcg aggctgcgct ccacttcata
      121 ggtgaaatcc acgtgccccg gcgtgtcgat cagatggaac agatactgtt tgccgtccgc
      181 cgcccggtag ttcatggcca cgggatggga tttgatggtg atgccccgtt cgcgctccag
      241 atccattgca tccagaagct ggtcgcgctg gaggcgcttc tggatcgtgc cggtgatatc
      301 cagcatccgg tccgccagcg tggatttgcc gtggtcaatg tgggcaatga tgcagaaatt
      361 gcggatgtgc gacaggttgc tcaagaattc agaccggtgt tgaaatgctt tgttgcgttg
      421 tcccgcatgg cgcggatttc cgcagccatg tcgtttgcgc ggtacagtga actcccggcg
      481 agaaatatgt ttgcgccgca ggcggccgag cggccggctg tttcggtgtc aatcccgcca
      541 tcaatggaaa tgtccatgcc cgggagccgc cgccggagaa ttttaacctt gttcagcact
      601 tcggctatga aggactggcc cccgaagccg ggatgcacgc tcatgatcag gatttcatca
      661 atctcgtcca gcaccccgtc aatggccgat gcgggggttt cgggattgat tgtgatgcct
      721 gcccgggccc ctttcgcgcg gattttgcgc agcgcgcccc ggatgtcgcc ctccgactca
      781 atatggatga gcacggtgtc cgcgcccagc tccacgcact tctccacata ggggtccggc
      841 ttgacgatca tcaggtggac gctgagcgga atgtccacga tccggtccac aacgcgaata
      901 atatcgaagc ccatgctgat gttgggcaca aaaattccgt ccatgatgtc cacgtgcagg
      961 ccgtccgaac cggcctccac cgaacggcgg cactcatccg ccagatggcc catgtcgcag
     1021 gccagaatgg acgggaatat ttctatcttc tgcataacaa cgcttctcgc ttaaaattca
     1081 ggtgcagagg ctagccgagg ccacggccat agtcaagatt ctccgccctg tccgtaaaaa
     1141 acccgccaca gaaagagccc ctgcggcgcc gcggtgggaa ctgcagccgt tctttttttg
     1201 gagcgcagaa tctcaccggc cgcctccggc ggaaggtccc cgttcccgac ccggatgagg
     1261 aaccccgcaa gactgcgaac cattttatac aggaagccgc ccgcgcgggc ggtgatgatc
     1321 acttcatggc ccttttttga aatcttcagc acagaaaggt tcctgacggt tccctccacc
     1381 tcgcggttcg ggttggcggt gaaagcggca aaatcgtgct ccccctgcag aatggccgcc
     1441 gccttcttca tggcctgcac atccagcggc cggcgaaccc atgttctttg attccgcaca
     1501 tgtggcggga ccacgttccc attccagatg aagtagcggt attccttgga cgcggcgctg
     1561 aaccgggcgt gaaactccgg cgaaacgcgg ctggctttga ggacacggat atcttcatcc
     1621 agcagcgcat tgcatccctc cagcagtttg cggacaggca tcggatggtc gaggtccaca
     1681 tgggccgcct gcgcgcgcgc gtgcaccccc gtatcggtgc gcccgctgca ctggacacga
     1741 accttttcct gaccggaaat cctgtggatg gcgttttcga gctctccctg tatggtccgg
     1801 ccgtccggct gtatctgcca gccggcatag gcggttccat cgtaggcgat gatgagccgg
     1861 tagcgggtca tgcccgcggc tcctcgtcaa agggatcgga gggctgcgca tcgagccagt
     1921 gctgcagaat gatctgggcg gccaccttgt ccacaaggcc cttgcggttt tcgcgccggg
     1981 tgcccgcttc aatcagcgca tgctgcgcgc tcaccgtgct gagccgctcg tcccatgtaa
     2041 gaaccggaat ctttgtccgc ctgcggagct cttccatgaa gcggttcacc ttctgagcgg
     2101 cttcgcctga agtgccgttc atgttcagcg gaagccccat cacaatctgc gaaacatcgt
     2161 gcttttcaca gagtgccaga atctccgcca gcgcatgcct gaggccggta ttgtcaatga
     2221 cacagagcgg cgaagcgatg atccgcatgg gatcgctgag ggcgatgccg attcgccggt
     2281 cgccatagtc tatgcccaga acgcgcgaca tcagagcagc ggcccgtatt ttcccgaatc
     2341 ccggagcttc tggacaagtt gtttgatgtc ctgcgccttg tcacgcgcgc agatcagcgt
     2401 ggccccttcc gcctgaacga caatcagatt ctccaccccc accagcgcgg tgagccggcc
     2461 gcgcgagaaa acaatgctgt tttccgcacc cacggactcc atctggccga tcacggtgtt
     2521 gccgcggtca tctttgggga aatgattctc aagcgcgggc caggaaccca catcgtccca
     2581 tgcgaaagac ccctccgcca taagcacgtt gtcggctttc tccataacgg cataatcaat
     2641 ggatatcttt tccagcgcgc caaacgcgtc cgccaaagca tccttgaagg ctcctgttcc
     2701 aatggccgga gccagattct gcatcatctg atacagcggc agacgatgta tgcgaagggc
     2761 cttttcgatt gccttcacgg accagatgaa catgcccgaa ttccaggcga attttcctga
     2821 cgaaacatat tcggcggctg tcgcggcatc aggtttttcc acaaagcgct cggccttcaa
     2881 aaaacgtgtc ccggcgctgc ttttgcgaaa ctcctcgccg gcctcgatat aaccataggc
     2941 cgtgctaggc tcggagggcc tgattccgat agtgatgagc acatcctcct ccagggccag
     3001 ggaacaggcg tcggccagtg tctggcggta cacatcaagg tctcccatga catgatcggc
     3061 ggtcagcacg gcaaaagcgg cgttgggatc gcgcttttta accagtgccg cgcccagcgc
     3121 gatcgccgcg gcggtgtcac gcccgaccgg ttcaccgaca atgttttcag ccggcaggcc
     3181 tggggctgac tcccgggccg ggccaatcaa atcggcattg gtaaggacaa acacgttgtc
     3241 cggggcgatc agcccctcca gccggtcaat agcctgggcc aggagcggct tctcccccac
     3301 cagcgaaagg aactgcttgg gcacgcggga tgtactgagc ggccagaatc gctccccttt
     3361 ccctccagca agaatcactg cgtaaaaatt gcttctgttc atttcctgat ctcctttgcc
     3421 gcccggacca gttccttcac aaaggcggcg gcgcgcgcgc ggccgcgcgc gtcgtgcgac
     3481 tcccggttga accggtccac gatcgcgctg ccgactacaa tggcgtctgc aaaatgcgcc
     3541 atttcacggg catgacgcgg ttctgaaatt ccaaacccca gggccaccgg cagatctgtg
     3601 gatttcttgg tctgcatgat cagttccgct gcgccagatg ccaccgtctt ctgctgccca
     3661 gtcactcctg cgcgcgaaac acagtacaca aaaccggtgc agtatgccgc aatctttgcg
     3721 attctttccg gccgagtcgt gggcgtcacc agcatgatgt tgttcagctc attccggtcg
     3781 aggatcttcc tgtagggggc agactcctcc agcgacaagt ccacgagcag caccccgtca
     3841 atccccgcgg tcttgctccg tcgcatcatt tgatcaaaac ccggcgcctt cagggggttc
     3901 aaataggaat agaatatcag cggaatatct gtattttccc ggacccgctc cacgcatgcg
     3961 agaacgccgt ccagcgtggc tccggcctgc agcgcgcgaa atgccgcatc ctgatttgcc
     4021 cgcccgtcag ccatcggatc ggaaaacggc acgcccagct caatgaaatc cgctcccgca
     4081 tcagcaagac gcagaatgat ttcttcagtt cccgccaaat ccggatcacc ggccgtgata
     4141 taggccacga atcccgtctc cccgtttttc ttcaactcag caaatttccg gtcaattcgt
     4201 gtcttaaact tcatgtctgt ttcgcctggt tcaattcgta aatatgccct atgccgaaaa
     4261 gcgtgagcga gtggtcgaaa atgatttcgc gatccattcc gtccagcacc cacggcgcaa
     4321 atccgcctgt ggcgcaaacg gtgacgttgg cctctcccat ctcctcctcg acgcgccgga
     4381 gtatttcgcg caccatgccc agatagcccc agcgcgcgcc ggagcggatc gcatcttccg
     4441 tgctcttccc gataatcgtc tgggccggct ccggcttgat gtgagggagg agcgctgttt
     4501 tctcagccag ataatcaaac ataagcggca ggcccggcgc gatgactccg ccgatatagc
     4561 cgttcacggc cgacacgatg tcaaaggtca gcgctgttcc aaagtccgcc acgatgacgg
     4621 gcaggccgta acgcaccgcc gctccggcgg cattggccag gcggtccgcg ccaatggttt
     4681 ccggccgggg ataagtgacc ggaattccca gactgctttc gcaggtcacc ctcatgggct
     4741 gaaatccgaa cagccgttcc acggccccgc gccaggtgtc atttgcatca ggcacaaccg
     4801 atgccatggc ggcgccgcag ggagtgattc catcagcgga ctccatcagg cgggcatcca
     4861 gcagttcggc cgtggcgtcc ctgtgccgga gccatgtctg acgcagaatc tccccgtttt
     4921 cgtagagtcc cacggccacg ctggtgtttc cgatatcaat ggtcaggatg ggcttggtca
     4981 tttgactgtc acacgttggg gatttcagcg gaaccgccgc gggctatcca ctccttaacg
     5041 agggcgtcgc ggcggccctc cagcgcggcg gcataattgc ggtccagaca ttccagcagt
     5101 tgaacggaag cgtccatttc gctcagttca attccttcca tccggcagga ggtggccggg
     5161 gttttcagtt caaggctgct ccaatcctct tcagtctgct tcagattcag gccgatcccg
     5221 gccacggcga actccacata ctcgtcgccc agccggggtt cgatcagtat ccccgcgatc
     5281 ttccggccgg acaccagcac atcatttggc gccttgatgg tcacattttt caggccggcc
     5341 gcatccagaa cctgcgcgac cgaaaccgcg cacagcacgg atagatggcc tgtttcggag
     5401 gcaaaccatg ggggccggaa caggatggac atatacaatc ccttttcagc ctctgatatc
     5461 cattttcggt cgaactgccc gcgaccctgt gtttgttctc gggcaataat cacaaatcct
     5521 tccggcgcgc cgtgcgcgcc cattttcttc aggtgctcac tggtcgagga tatggagcgg
     5581 taggccagga ttttccggcc gatcagcctg gtccgcacac gggcgctgat cgcctcgcgc
     5641 cattccggca gcggctcggc aataacactg gcgtggatga cgggtccgtt ttcaggcatc
     5701 gggccactcc agttcaagcg acagatccgc ggtcggggct gaatgagtaa gacatcccag
     5761 cgatacggcg tccaccccgc tggccgcaac ggcggagata ttgctgagcg tgatgccgcc
     5821 ggaggcttcc agccgacact gtcctccgca gattctgaca cagcgcttca tcgctgccgg
     5881 aggcatattg tccagaagta tccactccgg tttggcggcc agcacctggc gcagatcagc
     5941 ttcggactca acctctattt caacggccag ccctggatat tttttccggg cggcattaac
     6001 tgccccgcac agattgtcgc cgcccctctt tctccacaga ttgcggtgat tgtccttgat
     6061 caggatgcgg tcatagagcc ccatgcgatg attgcatccg ccgccgcact gcacagcgta
     6121 tttttcaagc aggcggaacc cgggagtggt tttgcgcgta tccagaatca tcactttccg
     6181 cgcccgccgc acaaactgac tggtcaaagt cgccacgcct gacatccgct gcatgaaatt
     6241 cagagccgtg cgctcggcgg acaggatgga acggagggaa ccgcggactt ccagcacggt
     6301 cgccccggcg ccggcgcgcg ccccgtccgg aagaacggtc tgcgtcttca ggctgggatc
     6361 cacctttctg aagacagccg cggccaccgg cccgccggaa atcatgcagg gggttcgggc
     6421 cagaattacg gcgcggccct tttccgtcgg ggaaatgagg ctcttcgacg tcacgtcgcc
     6481 cgcccgtccc aggtcttccc tgagggccag cgcaatgagc ctgtctgccg ccgcctgcgt
     6541 ttcaactgtc attttcatat tcaatcagcc gcgttttttt cttcgacgcg gacggctgta
     6601 attcaggccg ccgcctttcc cgcttcgctc ccggatcacc ggcgccttca attccgccag
     6661 ctggtcgcgc agaaacgccg cgcgctcgaa ttccagcgcc gcggccgcct caagcatttc
     6721 gcgctcgatg tctgcgatga cttccgcggt attataatca actccgctct cgcgaatcac
     6781 gcttttttcg acctcggccg cctcctcgcg cgtcacgaga ctctcctgga tgcttttctg
     6841 gatgccctgc ggcgtgatgt tgtgctccgc gttgtaggcc agctgaatct cacggcggcg
     6901 gcgggtcaca tcaatcatcc gtctcatcga gtccgtgatg ttgtcggcat acatgatcac
     6961 ctgaccgttc acatgccgcg ccgcgcggcc ggccgtctgg atcagggagg tttccgaacg
     7021 cagaaagcct tctttgtccg catccagaat ggccacaagc gacacttcgg gaagatccag
     7081 gccctcccgg agcaggttga tgccgatgag gcagtcgaat tccttcttgc ggagctggcg
     7141 gagaatctcg acgcgttcaa tggcgtcaat ctccgagtgc agatactgga ccttgagtcc
     7201 ggtttcctgc agataggccg ccagatcctc cgccgtaagc ttggtaagcg tggtgacaag
     7261 cgtgcgttca tgccgggttg cccgcaaacg cacctcctcc atcaaatcgt caatctggtt
     7321 cgcgaggggc cggacctcca ctggcggatc cagcagtccc gtcggacgga taagctgctg
     7381 gaccatttca gcggactgct cacgctcaaa cggaccgggt gtggcggacg tgaaaatggt
     7441 ctggccggcg gccttcatga actcgtcgaa gttcatgggc cggttgtcca gcgcggacgg
     7501 caggcggaaa ccgtgctcca caagcgtgcg cttgcgggcc tggtcgccgt tgtacatgcc
     7561 gcgtatttgc gggagggtga catgcgattc gtcaatgatg gtcaggaaat cgccgcgaaa
     7621 atagtccagc agcgtgtccg gccttgatcc ggcttcacgg ccggacagat ggcgtgaata
     7681 attttcgatc ccctggcagt agccgagttc cttcagcatt tccatgtcgt attccgtgcg
     7741 catttttatc cgctgggctt ccagaagctt gttctggctc tcaaaccagg ccacccgctc
     7801 gcgcatctcc tccagaatgt tcccgatcgc cgcctccacc ttcgaaagcg gcatcacaaa
     7861 atgcttggcc ggggaaatcg aaatctcgtc cagcgaggcc tcggagtttc cggtcagcgg
     7921 atcaattcgg cgaatggcct cgatctcgtc gccgaaaaac tccacgcgga tgcccttttt
     7981 gctgtaggag ggcagaatat ccaccacatc gccgcgcaca gaaaatgtgc ccggagaatt
     8041 ttcgtagtca ttgcgggaat actggatgtc caccagcctg cgcagcactt catcgcggtc
     8101 ggcggattca ccccgcttca gatgcaccag catgctccgg taatcctccg gagagcccag
     8161 cccgtagatg cacgagaccg atgcgacgat gatcacatcg tcccggttga tcagcgaatc
     8221 cgttgccgcc agccggaggc gttcaatttc cgcattgatg gccgcatcct tctcaatgaa
     8281 cgtgtccgtc tgcgggatat aggcctcggg ctggtaataa tcgtagtagc tgacgaaata
     8341 ttcgaccgcg ttgtttggga agaagctctt gaactcggcg tacagctggg cggccagcgt
     8401 cttgttgtgt gaaatcacca gcgcgggctt gccccaccgc gcaatcacat tcgacatcgt
     8461 aaaggttttg ccggaaccag tcacgccctc cagcgtgata aaccggctcc cctgctccag
     8521 ccggcggcag atctgttcaa tcgcctccgg ctgatcaccg gtggggctgt acctggattc
     8581 gatctgataa acggccttct gcgacattgg aacaatataa atcgcgggtc cggcctcaac
     8641 aagcctcaac cggcgggatg caaacaaaag gttgaaccgc tgcagccggg atgctaggta
     8701 tcggtcttca attcatgatt acgatttttc agtctgttgc gcatcgcctg ttcggcccct
     8761 caggcgctct tctggatgcg agggttctgt gcgaagaaaa tctgtgtgag acctgctctc
     8821 tgacggcgag ttttctcctc gcgctcggag gagaccggga tgcgctgctg agactgcagc
     8881 gggcggaagg ttccaatgaa tatgccggat ttctccgcag gcatctggga acggttgagg
     8941 aggagattcg ccgcgcctgt gaatctccgg atttcaggaa acggcttcag gcgcttgata
     9001 agaatccatc ggacaccagc gcactccgga tggtgttctt tcccgagggc gcgggactgc
     9061 cggaaacccg cgaagaaaaa atcgaagctc tgcgccagag gagaaccata cggatcacaa
     9121 gcctcaaccc cagtcccctc tccgatcctg tccggcaaat gctgttcaca tccaatgtgc
     9181 ttctgacggt gcccctgtcc ggctcgtcgc ttgacgaaat caactgcacg gatgagctga
     9241 aggctcgcat tcgcagtgtt gcaggcgaag agcagatgta ctggtatgat catcccatcc
     9301 cggtcggggt gcggccagag gccaatgaaa tcctttatgg tctgcgcggg ctggacacag
     9361 cggcggaatt tgagcgcgcg cggggactta tgggccccgg aaaaattgcc tgtgcgcttt
     9421 cagtttctgt aacgcacagg gggcttcagg acatcgccag gcagtacatc gagtcagaaa
     9481 tccgcagcgc gggcggtttc cgcaatgtgg atgtttatgt tttcaccgag gctgacacca
     9541 gacgcctgat cagagaagtg ctcgtcccgg ccggcggaga tgaggggtcc ctgaaaatat
     9601 tcggggtcga tggcgaatac ggaaggcact acagttttct caaggctgtg gcggcgcttt
     9661 ggcaggctgc cgcagatccc ggcgtccagg ccactttcaa gatcgatctt gatcaggttt
     9721 ttccgcagaa agagcttgtg gagcaaacgg gaaaatcggc gtttgaacac ttcaagagcc
     9781 cgctgtgggg tgcgcacgga caggatgcgg acggattccc ggttgaactg ggcatgatcg
     9841 ccggagcgct ggtcaacgag cgggacattg atcggggaat tttcactccg gatgtctcct
     9901 tcccggacgg ccccgccgct cctgatgagc gcatcttctt cagccgaatg acgatggccc
     9961 tttcaacccg ggccgagatg atgacgcgat accgcccgga cggcattgac ggaaaaacgg
    10021 catgcattca gcgcatccac gtgacgggag gaacaaatgg aatccgcatt gacagcctca
    10081 ggaaacaccg tccgttcacg ccgtctttca tcggccgggc cgaggatcag tcctacctgc
    10141 tttccgtcct gctggaagaa ggtgaaaaac tccgttatgt tcacaaggac ggactgatca
    10201 tgcggcatga caaggaggcc tttgccggtg acgccattcg ggccgcatca ttcgggaaca
    10261 tcattggcga ctatatccgc acgctctatt tctcagaata tgcgcgcgtg cttgatccca
    10321 ggggccttga gcggatcaag agcatggttg atcccttcac cggctgcttc atcaccccca
    10381 ttccggtcat cgtggtcatg ctgcggttct gcctgaaggc tgccgggtat tttctggcgg
    10441 gcgacgggaa aaccggaact gaatttgttg aggcaaacca taaccggctg gagcgcgcca
    10501 tggcctttgc ccgtgaggaa ctgaagcagc agtatgagcg ggagcgggaa ggctggaacc
    10561 ggtattacga gctgcttgac cggcttcagc atgaaccgtc gctgaaaaag cgcgccggcc
    10621 aaatcattca agactgccgt gtccggactt gaacggcatc cgggcaaaaa gttccaatga
    10681 ctggaatatc cgccgcaaaa agttccaatg actggaactt ttcaattcaa aaaagcttcc
    10741 agttcttttt gcagctgatc ggcgctgtcc atttcataat ggatgcggaa ggcctcttcc
    10801 gcggacatgc cgttcatgac atccagcgtg aagggcatga ttttctcggc cggctgcctg
    10861 agcatcagcc agccgaagag cattttggag gtccggtaga atgcctccac ttccgccgga
    10921 tactgcgtcg cggcaaagag atcagccagc ggataaagaa catcccgctt tttgaagtga
    10981 gcccttcggc ttttcttgat gcccttgaag gcggcgtacc cgaattcctc ataccattcg
    11041 gccatgccct cgttcagcca gagcggcagg cgccggggaa agagccggtt gatgaccaaa
    11101 tgcgtcattt catgcgcgag cacactgccg ctttccgcgg tgccgtccgc ctgttgcaga
    11161 aacatctccg ggcctctgac tactgaaaag gcccactcct ctttcatgcc gctcgcgcgg
    11221 atgaactcct gccagtcctt tggcgagcgg aagatgaaga tgtgagaacg gaccgcaggg
    11281 ccctgagccg gtgcattcaa ttccttcgcg atgtgatcga acaggaattc agccatgcgc
    11341 gccgctttca gggcaaaggt cttgcgctca taatggtaca caaagcgctc cgtctgcgcg
    11401 tgctgccacc tgatccccgg catctgcaga acttcccgcc cgagaagcga gaggctgccg
    11461 gagtccatct gatccggccc gacttcctga aaatccgctt gtgcggaaac ggccagcaga
    11521 cagatcatca ggttcttcag catgcgaaaa ggtttcccgg attgaggcgg cactgcggat
    11581 cgaacagccg tttgagccgg cgcatgtcct cgatcccccg cgctcccatc attttaagga
    11641 ggaacggggt tttgagtttg ccgattccgt gttctgccga aaccgttccc cccatgcgga
    11701 tcacggcatc ggcccattcc agatactggg ctttcccctg ttcaaattgc ttcacggtgc
    11761 ggggaaggat gttgacatgc acatgattgc tcccgatgtg tccaaagatc acatattcga
    11821 gccccaggcg ctcaagcgat gcgtgataca gcgccatcag ctcgtccagg cggtcgtcgg
    11881 ggacggccat gtcggtgccg agtttcgtga tcgcgggatc ggttttccgg cgttcatcta
    11941 tcagagagtt gaccgcttca ggcagcgcat gacggaacag gcgtatgcgc tcccgctcat
    12001 gatcatccgt ggcaatccat gtcctgtcct cgcttccccc gcagccgtcc agcgcttcgc
    12061 acatgcgctc caccatgccg gaagcctcat cctcgtccgc cgcatgatac tccacataga
    12121 ccgccgtgtg ataatccgcc ggcacctcgg gggcgccctc cagcctgtgg ctgcggagca
    12181 ggttcaatga gcaggaatca aaaaactcaa tagacgcggg tttgtccttc agtccgcgga
    12241 cggcatgaac aaacgccagc gcatcgggtt cttttccgaa gaaggccatg accccccaga
    12301 cggaagcggg ccagggctgc agcttgaggt gcagctcgga aaaaatgccc agcgtgcctt
    12361 ccgaaccgat gaacaaatcc agcaggtcca tgctgtcgcg gacaaaatat cccgaagcgg
    12421 tcttaatggc cggagtggta taggaaggca ggtcgccttc aattacacgg ccggattccg
    12481 ttttcagcct gaaattccgc ccggtggcca tgcactggcc gcggcagagc gcgatgacat
    12541 caccgtctgc aagaacaagg cgggcggcga ggatagattc acgcgtggcc ccgtagcgaa
    12601 acgagcgggc gccggaagcg ttgcaggagg ccatgcctcc gatggtcgct gtggtctccg
    12661 tgggatccgg cgcaaagaaa acattccccc ggcttttcag gtgcgcgcgg atttcatcca
    12721 gcgtcacgcc gggctgcacg atcagatcat ccctgtccgt gatcgtcttc atccggctga
    12781 gattcaggat atgtccgccg gaaggcacag ccccgcccgt taatcctgtg cgcgcgccct
    12841 gtgtggtgat gcactgcccg tttgccgcgg cccagcgaac ggcatccgca atctctgcct
    12901 cgttcagcgg gaatgaaatt gagtctgccg tgccggtttt ccgcgattcg tcgcgcagat
    12961 aatcgggccc gatgtcactg tgaatctccg gcatgtcact tgtccgccat gtgaatggtt
    13021 tgcgtcggga aggcgaactc aagactctcg tcattgaact tgcgcaggat ttccatgttg
    13081 attttgtggc tgaaggccat atagccgagc cagtcgttct tcccgtacca gtagatcacc
    13141 ttgatgccga gtgaaaaatc attgaaatcc gtgaagaata ccagaggagg gaatttcgga
    13201 tccatgtatt catgattgtc cagaatgtcc ttcaggatct ggaccgcctt ctccatttta
    13261 tcgggcgggg tgctgtagac cagtccgatg ttgagttccc tcttcagaaa cggccggcgc
    13321 gatatgttgg tgatgctctt catggacagt tcgccgttcg ggacgatcac cagatgcccg
    13381 tttaaaagcc tgatttttgt tgaacggaat ccaacgcttt ccacattgcc gtcaattccg
    13441 tcaaaaacaa tgcgctctcc aagttcaaac ggcttgtcgg ccaggatcac gagcgagccg
    13501 aaaaagtgct gcaggctgtc cttggcggcg agcgcgacgg cgagactgcc gacccccaga
    13561 ccggcgagaa tggatgtgat gggcttgtcg ctgagtatct gcgcaacctg aatcagcgtc
    13621 aggaccacaa cggtgatgcg cagggatttg gtaaccatgg gcgcaagaat ctcgttcatt
    13681 ctgctggagg accgcgccgt ggcgcgcgcg agccccacct gcacggcatc ggtcaggcag
    13741 tagacaatga acccgaccgc acaggtggtt agaaccgaaa ccgtggtcga gatcagctgc
    13801 tgaaactgtt ccgtataaac aaggaaagac aatcccatgc gaaaaccgaa caaaaacagc
    13861 aggaagacca ttcctctggc tatggcgcga agcaaggcag aaaccgccgt cttttcgcgt
    13921 gccgccatga cttccgatct tttcagcagc acatggcgga gaattcgccc tgtcagcagt
    13981 gccgcgagca caaccacaaa gaaactcgct atccgccaga tgctgtttcc ggccagagtc
    14041 cagttcatca tttgtgtcag tcgttccatc agatctcctg ttttttattc atataaacga
    14101 aaccggcgga tgaatatcaa gctcttcatg attcgaccct tgccgcgagc ggaagaaatg
    14161 cgcggcgcag gtgtgcagag aatccctgcc cggaatcatg cagtcggtca ccgcgttttg
    14221 tttacgggag cgtcaccatg gtgcggtaat agcccgcaga gccgttgttg gtcacagaga
    14281 gcacaacggc ccctccggtg cccggcaggt tcagattgag ccgggtccag ttgaccatgc
    14341 tttccgtcag attcgtgctc cagacaacat catagacccg gtcgcctgaa gtgggcgggc
    14401 ctgctgtgat gttcaggaca gtcctgccga cggcgctggt gatggtgttc tgatagaagg
    14461 acccgtcatc cagcggatcc gtgtcggtaa tatattcgta atggttgctc ttcccgtcac
    14521 catcggcatc gtcagccggg ttgttggtca gactggaaaa gcggttccac tcccagtcgt
    14581 ctccgatgcc gtccgcgtcc cagtcggccg gcaccacaat aactgtcgag tttgtcacca
    14641 cgctgctggc ttcgccctgc ccgtcgttcg caacgaacac caccaggctg gtcgagccgg
    14701 cgtccgatgc gccggcggtc catgagaacg tcgtgccgac ataggttgcg cctgcgggcg
    14761 cgacggtgtt ggtcagcgtc accggatcgc tgtccggatc gctggcggtc acctcgaagt
    14821 tcgtgtaagc gccgactggt agaatgtgag gcccgggcag gctgatgacc ggcgcatggt
    14881 tctcatccac ctcaaccagc ttcaagtact gaaccagttc cccgtcgttt gtgatggagc
    14941 cgccggcggt tcctccgttg agctgcacga atattccgtc attgtacagc gacgctccgc
    15001 tgatcggcga accccagact tccgcggacg catcactgga ggcaagattg cggacctgat
    15061 aaagcgttcc ggtgtcgaga cccagaagat cccacgcgcc ggtcagatca taggtggcgg
    15121 aagctccggt gtgcgcctcg ccgtgccgga acatcagcgc aaaggccagc acgacatcct
    15181 tgtcgttcgg cgaagcatag ggttcttcat atttcgccac cgcgaggatc cttccttctt
    15241 caccgccgcc aaccttgctc aagaaatacc ggttctgact ctgaagcgcg gggctgttca
    15301 agcgggccca gttcacgcgg ccgtaccact gcgcaaggcc ttcggcattc ggcggcggat
    15361 tcgaccagac catgagctgg ttccactgct tgaagtgcgg aatgaacttg ccgaagtttt
    15421 cctcatgatc ggtgcggaaa ccgtcatagt accagtagga ggggtcggca ttgtagttct
    15481 gaatgccctg ttcctgtccg tagaagatca tcgggatgcc gtccacggtc gaaaccgctc
    15541 cgtagcggct ggcgaccagc cagcagtcgt tgtcgggcag gacttcatcg tgcgatgtca
    15601 gattcagaag caccgcgccg ccgttatatg cattgcgccg gtcttcgagg gcggagcgga
    15661 ccgaccacga atcgttgatt tgcgcctgcg tgaactggaa aaccaggttt tcattaagca
    15721 catcaaaatg gcggttcgac cggtatccgg gtttgccgcc gtccagcgtt tcggccatga
    15781 aaatgaaatt ccatttcatg gagcgcgtgc ggttgatgat gtactcccag gtttgcggcg
    15841 ggagcccctg cccgaagtcg cagcggagcc cgtcaatgcc ccggtcatcg tcggcccgca
    15901 caaaggtgtt ggtgccggaa tgaccggtct tcttcagcca gtactccgtg tagtatccga
    15961 aatatttcca cagatcgatg gtgtccgcgg acatgcccgt gtaatcgtag acatcgtcct
    16021 cattcagata atccccgttg ttgtcgggat tgtggcgcac cagagcggaa tattttccaa
    16081 aatagaattc ggccacatcc gcccatttgc cgaaatcgcc gcggtcgggc gcggtggcga
    16141 aatcgttgct gtagactgaa tgataatacg tggccggctc gccatagtcg gtccacaggg
    16201 cataccagcc gggcttgaac cagccgatgt gatcgttttt gttcgtgcag aatcccagat
    16261 cgagaccgcc ctgccccatg accgcgtccc aggccgtgtg attgaacacg ccgtccagca
    16321 tcacgttcac ggtgcccacg gaaccggtat aggcatcaca cgcggccacg aaattcgtga
    16381 actccgccag cgcgcccgtt tccgttccgt ccgcgccgag atagggcgac acggcataat
    16441 agtcgcgtgt cgcatagggg ctgcccggct cgtattcgga ggggtcgccc cggaaggtct
    16501 ccgcgctggg atggatcggc tggaaccaca ggcagttggc ctggatgtaa ttgagatatt
    16561 caagattgaa cgggtcgaag ccgtcggtgt cgccttccga ggagcccagc aggtcgcgga
    16621 aggtgctgcg gcccgcctcg tcattatacg tcgcctcaac agtcagaggg ttgagttcgt
    16681 acatcgtcat ctccagcact tttttcgggg aaacgacgac ggcgtgatcc cggcggcctg
    16741 cggatgaata ccagtgccag gagccggccg ccatgccgtt ggttgaatat cgcgccgtaa
    16801 ggcggtacgc cccgcacctg ctcaccgtgc cggtccagac ataggccccg ccgccgacag
    16861 gcgtcatcgg atacgccctg aaatacgcgc ccgtgtcgtt ggtggtgatc agatcgcccg
    16921 aaggcggcct gatcccgtcc ggaacgccgt cagtgttgta gtccgcatcc gcatagtcgc
    16981 gccggtcgag atttgaaaat atctccacct tgtcaaggtt cagcgcgccg ggaacatacg
    17041 tcacaactat ctgctccgtg tcgccgttca cttcatcaat gaagaacttg gtcgtggtgt
    17101 aatccgcatt gagcgtgccc acggtgagct gctgggcccc cgcggttccg attgcgaacg
    17161 agaacgtatt gctggctgtt cccgctgtgc cgtagtcggc ataacgctcg gtgtcgttcg
    17221 tggttttcca cgcgccgatc ttgtacttga tggtcgtcag ggacggcagg ccggtcgcgg
    17281 ttccctgcca ccacatggcg tcggcatcgg gatagctgtc ctcctccatg tgatcgaaac
    17341 tcatcgccac gacctttgtg gaggcattgc ccgccgcgcc gtaatgcccg tgcggctcgg
    17401 tgccgttggt ggtgtaataa acggccgcat aatcggccca ccggttggtg ccgatgccct
    17461 gcgcatagcc gatcttggcc cagaactggg cgctggttcc cgacaccacg cggttgtccg
    17521 catgccagac aaattcaggc gactgcccgg cggcggacga ataggtgaaa gtgaagggat
    17581 tgccctgcgc gtcaccggca tcggcaaagg tcaggctgga agctccgctg ttcgtggtgc
    17641 cgatccaggt gtcgtcatga tcgttgtacg taatgagcag ataatactgc acctcatcgg
    17701 atgccgcata ggttccggcc ggaatcaggc cgacccagta cttgttgttc gagtccacgc
    17761 tgtcaaagga aagactgttc gaactccacg agccggcgtc cgtcttttta tggaagagcc
    17821 agccgcccgt ctggtcgccg ggattgcccg ccccctgata ctggctgccg ttgtatatgt
    17881 agaccgcctg atttgtggag ggatctccgg ccggattgcg catggtcgcg gtgaagggct
    17941 ctgtgttcgt cgggatatgc cagcagtttc cgagattggc cggaggagtt ccaaccgaat
    18001 actggtagtt tgtggcgccg gatttcttcc cgcgcagcgt gcacagatca aaatcggccg
    18061 tgatgatatt ggaaggcatg gttgaggtca gaacataata ctcaatgact gaccccgtcg
    18121 gactggccgg aatcgcggcg gtgtagttcg tggagcttcc ggcggcctgc gccgtgaggt
    18181 aactgttcgt ccatgcgttc ttcgtgtagc gcacccagac attctcctgc gcgcttttgg
    18241 ccgcgctgag ctggatggtg accgtcaccg cattggtggc ggccgtgtca tgattgtcgg
    18301 agaccgccag caccgatacc gggctgccct ccgttcccat gataacatag ggccggtccc
    18361 accactcata tgttcccttc aggttgaacg tgtaccgcca tccgctggtt tcgctgaagg
    18421 aaagatcgga ggggtcgccg ccgtcattga tgcgggcctg gccgatggtg gagttgacgg
    18481 cggcgttggt tgagtaggcg gccttgtatc cccactgcgg attccagttg ccgctttgat
    18541 caaacttgag gccggatgtc gcccgcgtgg ccgtggaagt gatggtgacc ttgtagatgc
    18601 tcccgaaatc ggtgtcggcg gtcatccagt tggtgcccca cccgttgatg tcgccgcgcg
    18661 gggtttcggc aaaaagcccc tgaaccgcgc agaacgccgc tgtcaaaaca acatgccgca
    18721 gtgccctctt tttcataatg attccgtctc ccgctgatcc tctggaacta ctgtccacaa
    18781 gatgcatcaa aaacggacag tcttcaaacc taatccgcga gcatctcgcg ggatcaccgg
    18841 gactaaatct gccggaccgc ttccgcctgc cgtgatgcaa aatagtacta atttagtact
    18901 atttagatcc gcacggtgca gttttgcatt ttcccgcgcg cgcccccttc atttcccctt
    18961 ttttttcatt tttcgcggcc gcaagtgccg ccgggctgat attttcatga aaattcgaaa
    19021 attcaaggat ttttggcctt ttttgacgtt tttgggccga tttttggtca ttttttgcat
    19081 tttttggacg ttttggggct gtttttcgcg tttttttgag ttttttacat ggtatggccg
    19141 ccggggcaat cgttgcgtga aaatagtttt ttgtgtataa aagaaggtca ggaagtcggg
    19201 tgcatgatgc caagcggaaa aactgcagaa ggaccgcgtc gcaagaacct ggacgggctt
    19261 ttcaaacccc ggagcgtggc catggtcggg gcgtcagccg atccccataa aatcggccac
    19321 cagattttga gaaatatcgt cgaaggcggt tttgccggag aggtgtatcc cgtcaatgcc
    19381 tgcgccgagg ccgttctggg cctgaagact tttccttccg ttgcggccat cccccacccg
    19441 gttgatttcg ccgtgatttc catccccgca accgccgtcg ccgcctgcat ggaggaatgc
    19501 ggacagaaag gcgttcaggc ggtcgccgtg atcacttccg gattcgcgga ggcgggcaac
    19561 attgaagagg agcacaagct caaggcgatt gcggaccggt acggcatggc gctggtcggg
    19621 cccaatattc tcggcgtggt ctattccccc tcccggctca acgcgacctt cggcccgcgc
    19681 gacatggcca gcggcggcac ggcctttgtc tcccagagcg gcgcgctggc catcggcctg
    19741 atgggctggg cggtgatgga gaagatgggc atgagcgcgc tggtcagcat cggcaacaag
    19801 gccgatgtgg atgagcggga tctgattgag tacttcaatc atgatgaaca cgtgaaggtg
    19861 accctcgttt atatggaagg cctgaagaac gggcgcgatt tcatgcagac cagaatcagc
    19921 aagccggtcg tgatgctgaa agtcgggcgc acgcagcgcg gcgccagggc cgcggcctcg
    19981 cacaccgggt cgctcgcggg ttcggatcag gtctttgacg ccgccttccg ccagctcggg
    20041 atgctccgcg cggacacatt ccgcgaggcg ttcaactggg cccgggcgct tgccctgccc
    20101 ccgatgcccg gcaggaaggt gctgatcatc accaatggcg gcgggatcgg cgtccgcgcc
    20161 acggacgaat gcgagaaggc ggggctgcac cttctggatg atcccgaatg gctggaagaa
    20221 acattccggc ccgtcatgcc gtcctacggg agcacgaaaa atccaattga tctcaccggc
    20281 ggggccgcgg cggacaaata cagccaggcc ttcaagtccg cggtgatctc cgacaagttc
    20341 gatgcgctgc ttgtgctcta ctgcgagacg gtgatcaccg acccgctggc cattgcgcag
    20401 ggggtttccc gggaatatga ggccgcaggc cgccgcaagc ccgtcgtggc cgccatggtc
    20461 ggcggagaac gctcgcggca ggccattcat ttcctgaatc agcagggcgt tcccgccttc
    20521 acatccgtgg ccgatgcggc ctcatcgctg aacgccctgg cccaatggca tgaaatctcc
    20581 agccgcgaga aagatgatcc ggagtgctcc caggcgcccc gggaggccgt caggattgtt
    20641 gagcaggccc gaaaagaggg ccgttccgcc ctgctcgagc acgaggcgcg gcaggtgctg
    20701 gaactctgcg gcgttcccgt gccgcccgcg gctttcgcgc gcgatctgga tgaggcgaag
    20761 cttaaggccg cagcgcttta tccgctggcg atgaagattg cctcgccgca gatcattcac
    20821 aaaagcgatg ccggcggagt ggccgtcaac atccgggacg aaagcgagct ggctgaaaaa
    20881 tacagcgcca tgatgaaaac cgtttcggag cgcgtcccgg atgcgcagat cgagggcgtg
    20941 aaccttgtgc agatggcgga gggaatcgaa tgcgcggcgg ggctgagcag cgatccgcag
    21001 ttcgggcccg tggtcatgtt cgggctgggc ggcgtgctgg tggaggcgct gaaagacgtg
    21061 gccttccgga ttgtgccctt cggccgcgtg gaggccgaac gcctgattgg cgacatcaaa
    21121 gcccggaaca tactcgacgg attccggggg gccagggcgg accggccctc gctggtgcaa
    21181 atcctgtgcg ccatccagaa actggcgggt cttgtgaagg aagtggacat caacccgatc
    21241 cgctcaagtg aaaaaggcag tttcgccgtg gatgcccgga ttat
//
LOCUS       DAOQWR010000027        21277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2841_length_21277_cov_5.840637, whole genome shotgun sequence.
ACCESSION   DAOQWR010000027 DAOQWR010000000
VERSION     DAOQWR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_21277_cov_5.840637"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKK36_03670"
     CDS             <1..695
                     /locus_tag="PKK36_03670"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNR93690.1"
                     /translation="LKTCPLDQKKFFIRAVLRQGIEEVAVEIVGRHDRVARVLLNGRV
                     FYREEPGPVFDISMNEIWAVVEKRDPALEEIALDFIMRQVRGDKTGTADQNVPGLVRA
                     RMLGSDAPVMTVTGSGNQGIFIGVPFYELYQEMGKAILPAVLFALLTQIHLTKKASRI
                     SGACGLATKAAPALTAGLMYAQGASLPEICKAIENLPSRIGYIPCPGAMASCGTKARK
                     VLKHVLPQLEAV"
     gene            692..1549
                     /locus_tag="PKK36_03675"
     CDS             692..1549
                     /locus_tag="PKK36_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR93691.1"
                     /translation="MKNIKITIVVENTASMEGLLAEHGLSVWIETPNANILFDTGQGP
                     ALPQNAAALKIPLERANAIVLSHGHYDHTGGLPHALERAPKAKVFLHPEAFAPRFSIR
                     DTIMRAINMPYPAEGALKSHSGNIVHTAKSMEVVPGVWVTGAIPRRNKYEDTGGPFFL
                     DKLGLKPDPIPDDQALWLDTADGTVVVLGCAHAGVVNTLEYISELSGASEYFAVMGGM
                     HLARATVKRLGKTAEALERFHVKQIAAGHCTGANAIEFLARRLPGRMIPCAAGSVFAF
                     DGSARQNGP"
     gene            1629..2828
                     /locus_tag="PKK36_03680"
     CDS             1629..2828
                     /locus_tag="PKK36_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR93692.1"
                     /translation="MKISKHARHHYRKRNHDDPLTPYGNRPMVTPIVNAVNYEYLDFD
                     VLQKITDGKIDGYTYHRDDNPTVRAVEKRIAELEGAEDCVVCTTGMAACSMVYLTYLR
                     AGGHLILFHDIYGAHYKVSLILERLGVEVTWVDAADWKSVKDCIRPNTQMIFLETPSN
                     PLCKVIDIAATKKMARKVGAMVVVDNTFATPYHQHPLELGADLVVHSATKALGGHNDL
                     MAGAIAGSKEDYDKLWFTRQAIGTTLDAYSASLLERGLKTFPLRAERMASSAMAVAKH
                     LAQCRGISGLHYPGLKSDDGRRISARQMEHGFGGMLAFDVGKTQEDAKRFISALRVIR
                     HAVSLGSTESLICIPFLTTMLYMPPERRTLFGVKPNTVRLSVGIEPAAEILADIDQAL
                     KKVGKRK"
     gene            3012..3452
                     /locus_tag="PKK36_03685"
     CDS             3012..3452
                     /locus_tag="PKK36_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNR93693.1"
                     /translation="MDKKLLAERMASFPELCLRAGIRVTHQRTEVYREVAATLEHPDA
                     DSIYRRVRRRIPQISLDTVYRTLGLFGEKGIISRVSYPGDRARFDGNTGQHHHFICTK
                     CGLIRDFRSDALDAVRPRLKNVPCGRITSVQIELRGLCRRCARA"
     gene            3596..4279
                     /locus_tag="PKK36_03690"
     CDS             3596..4279
                     /locus_tag="PKK36_03690"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNR93694.1"
                     /translation="MTEQNAEVRMPLIGDKAPSFRAVSTQGEIDFPKWGEGKWVVFFS
                     HPADFTPVCTTEFMTFASMAEDFRKRNCELLGLSIDSIYAHIAWLRTIKEKIEYNGMK
                     DLEVNFPVIEDLKMDVARAFGMLQPGASSTQAVRAVFLIDPKGVVRMIMYYPSSTGRN
                     MDEVMRVLIALQTADKHGIATPANWQPGDDVIVPPPGSCGTAKDRTAKPDPDVKCLDW
                     FMCLKKCPK"
     gene            complement(4361..6172)
                     /locus_tag="PKK36_03695"
     CDS             complement(4361..6172)
                     /locus_tag="PKK36_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNR93695.1"
                     /translation="MFKKCSIVTGGMILLAGLAAAANGGPGEALNQQLIAAAGDPAGM
                     ERIAELLAQGTDVNATDEDGCPALMSSAESGHTAVAEFLLSKGARVNTSDVYGWTPLM
                     VAVRRGHAETVKLLISKGAKVNVSALNGWSPLLDAAEKGRTEIVQALLAAGAKANMKD
                     DTDWTPIMFAAKGGHTEVVKALLARGARVNAKSSHGETALIKAVWGGHDETVEALLAK
                     GADVNARGMSGDPAVLIAAAEGHTRIIEKLISRKASVDMAAALTAAARGGHTETVQAL
                     LSKGAEVNLEPVLMAAVEGDHFSTVKFLLDAGAGGSLDKALNVAAARGHAKTAEVLLS
                     HGADVRAADAHNRTALKMAALSGNPKTAEALLAAGAGSSAADLLAALEAAESRGQAGV
                     VETLLRKAVDLPEKSSPVLPFSARGGYIDAVKALLEQGADVGTKDANGKTSIMLAANR
                     GHTDIVKILLDHGADVNEAAGKSHVESGTALYEAARGGEHVEVAEMLLARGADINAKN
                     NFGRTALSAASSRGHAKTAAALLAHGADVNIQDNFGVTPLMEAVKIGNLEIVKALLNR
                     GADTHIEDNYGRTALSLATPGGPPEMIRMLEQAGPAK"
     gene            6379..7788
                     /locus_tag="PKK36_03700"
     CDS             6379..7788
                     /locus_tag="PKK36_03700"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNR93696.1"
                     /translation="MRKRDTFDFLTSTAGVIADAAGVYAGFMLAMWIRFFSGWIPLHG
                     ESLPPMFQYLVGAAVATVLFVLIFRAMDLYRRPQHGHFTEKMPRIVRGCAYGLVISTA
                     MAFAVRNPPFSRVAMILSFFTVTLVVLIERNILFQCERHWARHQAAKKNVVICGIGPI
                     AGQLTEILSREHRLRAKPVAYFSVGDETADPRVPADLVRGPMNGIPDFLGANDVDEVI
                     VANPSGLTHAQLVDLIIQCEKALADFQMVPDIFGVLTSRVDMQMVGGIPMLGVGKWPL
                     DYFWNRARKRAEDIAGAIVGLIISAPIIAIAALVIRKTSPGPIFYRQERCGERGKVFN
                     IYKLRTMHCDAESQTGPVWASPDDPRRTRFGTFLRKYNLDELPQFWNVLTGEMSLVGP
                     RPERPHFVEQFKEDIARYMWRHVSKPGLTGWAQVNGLRGQTSIEDRISYDLFYLENWS
                     LSLDFKILIRTLFSTKNAY"
     gene            7904..8188
                     /locus_tag="PKK36_03705"
     CDS             7904..8188
                     /locus_tag="PKK36_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR93697.1"
                     /translation="MPTYEYECQKCGLRFELFQSMKDKPRQRCPTCRGKVVRCLGTGA
                     GIIFKGSGFYSTDYRSPTYHAAAKKETAAPSAGGETKSSGKAEKSHATNS"
     gene            8172..8864
                     /locus_tag="PKK36_03710"
     CDS             8172..8864
                     /locus_tag="PKK36_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR93698.1"
                     /translation="MPPIVKCKTCGSENILGQLFCKQCGARLKMGGTVEQKKPKGSGL
                     RIVKTLLRIAVLLACIALVGGLLWPAQPSGEFGSERHADVYEEKIGRLEQAAREGYST
                     VEEIAETQINGYFARLVTRNSGEGDSASQLGIGEINFAFRQDTVIVFVLARRGPLTLT
                     YELTAAPVADKGLQLNVQQVRLGRIPLPGFLHQRILQRMSVVFSQMDAEQLILRHLQK
                     IKLDDKRAILTT"
     gene            8883..10598
                     /locus_tag="PKK36_03715"
     CDS             8883..10598
                     /locus_tag="PKK36_03715"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HNR93699.1"
                     /translation="MRDKKEFHAILTQISGGLFREYGRLAGDFDFNRYVLKIHFVQDA
                     AEDGPTLFVVRVPQFISEFPERLFSSAVRRTALEDLLVRKISESIEGMAHFNSSGTAR
                     RRFQIARLGQKILPRSSVIVTDEYVEARLYASLPREGEVIPGDAVLELFFEDLPAIVN
                     TALIYCNLDRDEVEHTVGLMEDTDVIRQMLPTQGMVGFVGDGLLLSRKPGTDLPDFTR
                     NTSLKVDVSARLEVEVPHLSKVYGAGIPAGITVIMGDAYSGRIEIMQALASGIYNHIA
                     GDGRELAITVPDAVEIRSEPGRSIAQVNVSAFVSGHACGCAPEACSTNSADAFLSQAA
                     ATVEALQAGARVLLFDEATSCSAFLSSDQRLTALLGARSGIIPLAARARQLADDLGVS
                     LVIGGETCVAEFIPIADRVLRVNRNVISDITEEARKATVAQPPPRAAAIDVAALAEQQ
                     RWIVPSSIDPTLGREDSYICAPNVHTLCFGRSTIDLSAVSQLADIQQTETIAHILYYA
                     KLRYMDESHPLREILDLVDRDLSTEGLECLARELRGDLARPRRYEIAAALNRLSTLRV
                     QNPTD"
     gene            10607..11053
                     /gene="dtd"
                     /locus_tag="PKK36_03720"
     CDS             10607..11053
                     /gene="dtd"
                     /locus_tag="PKK36_03720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNR93700.1"
                     /translation="MKLVIQRVSRARVGVEEKTVSEIGPGALILLGVARGDTPADAEW
                     LAGKVSRLRIYDDPRGRLNHSIAETGGSYLVVSQFTLFGDCSKGNRPSYIQAADPDDA
                     AALYDLFVEKLRALGHTVRTGVFREHMDVELVNDGPVTLLMESCGR"
     gene            11043..12203
                     /locus_tag="PKK36_03725"
     CDS             11043..12203
                     /locus_tag="PKK36_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93701.1"
                     /translation="MGAERNILRLVLSALLCGAAARLPAQETRDRRVVSATGRFVALS
                     PAGQDATYLVVWAEDVVERLQKLLGMALPVPRGEALYLIVGPGEGAISAEQYYDEFGL
                     RQKLHISGIEAADQEQVLEHLCRFLLNRQTLPEQVPFWFAAGAAQNLFPEARVRNMET
                     AISEWRADRAPDFSDITGGDWPDTPDFRTRAFAGLAVAALLEWFPDSARTEQFLSACA
                     AGQTPAAELAAQLTGRGSVRDLEAAWDVWLASRQNIRIVGAGSPRQDAERLRAMLEIT
                     PVMIQLVAGRKVPGEFSAQRMIAERAEDWVPAVARHLDLKLESFSLGCAPETQGVIRA
                     WRDFLQTLSVRDEKRPWYSLRKAPAVSERELLVKLARAEQLLFELAEAASDE"
     gene            complement(12210..12977)
                     /gene="budA"
                     /locus_tag="PKK36_03730"
     CDS             complement(12210..12977)
                     /gene="budA"
                     /locus_tag="PKK36_03730"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNR93702.1"
                     /translation="MKNLLLAAALALLAAGCATTPDNTVFQMSTIDALLVGVYDGHLS
                     GRDLLRQGNFGIGTFEALDGEMVVLDGRIYQVKADGSVVEVNHDASTPFASVCFFNPD
                     IRFDLTPASDFAAIEEQVNRIAPNQNLFCAVRISGKFKSMHTRSVPRQQKPYPPLKQV
                     TARQPEFFMEDIEGDVVGFRCPEFVKGVNVPGFHLHFISRDRTQGGHILGFELAEGRG
                     EVDLLSRFHLQLPQDAESFAAADLSRDRSEELHAVEK"
     gene            13252..15756
                     /locus_tag="PKK36_03735"
     CDS             13252..15756
                     /locus_tag="PKK36_03735"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNR93703.1"
                     /translation="MANSAFAETAPAGARQIPGWWYVCPVASVVALLMAFFFYRFIMK
                     SSSGTDRMQEIAGYVREGAYAYLRRQYKVVGLVFVVLVAVLSFMAFGLGVQHRLVPFA
                     FLTGGLFSGLAGFIGMKTATAASVRTAHGASESLNKGLTIAFRAGSVMGLTVVGLALL
                     DISLWFLYLYKYHHIVTGGHEMNLETITVVMLCFGMGASTQALFARVGGGIYTKAADV
                     GADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYAGSVLATAALGVA
                     MASVHGFGVQKAFIYLAAPMLLAAMGILFSIIGIFVVKTRDDANMKQLLSALNRGIYL
                     SSVLIIAGAFALVRVMGLPTGVFTAITIGVVLGIVIGKGTEWATAAEYKPTRYVADQS
                     LTGPATVIIGGIAEGMMSTWIPILAVCIGTLCSFAVMDGFNDILAGLYGVGIAAVGML
                     STLGLTLATDAYGPIADNAGGNAEMSGLPKEVRQRTDALDSLGNTTAAIGKGFAIGSA
                     ALTALALLASYIEEVRVGIHREAAIEYAAPAAEESYTVVNVGAADNGKYAICLPGAAA
                     GSRQSQQFYTLSAFEGTGERAVRVSGPDGQPVSLIVNEVGEMVKDRGAADLDGRLLCA
                     QKAGLMDYMNFYNVTLMNPKVLVGEFIGVLLVFVFSALTMKAVGRAAFAMVTEVRRQF
                     KESPGIMAGTERPDYARCVAISTAGAQREMIVPSLLAITIPVLVGLFLNVSGVVGLLV
                     GGLCAGFAMAVYMANAGGAWDNAKKFIEAGNHGGKGSLAHKAAVVGDTVGDPFKDTCG
                     PSLNILIKLMSMVSIVFAGLIVKYAPAIGAWLGIGN"
     gene            15775..16539
                     /locus_tag="PKK36_03740"
     CDS             15775..16539
                     /locus_tag="PKK36_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93704.1"
                     /translation="MDIHLYLVCYQSEALVASHLDAAAFGAHLAVGTQKHTSGNVMFV
                     EIDPSFKSDALKLNKLSSRCIPHADGSPRRSKYISIYRALEHIPLSAFGKLYMTTRDG
                     RVLGLDSKPYDPESEEPGTNLYTELCPVFPRVASSLPPFRFCRFITNPDNPLHVPRII
                     FSDNLLDREDDGSLAGYLPYPNREHIVDCIRALTSSEGKPTKTIERNPQLIAFYRTVG
                     RGFFAGDQTDMIFYPFPSRAELEGEHHLWWRSASMD"
     gene            complement(16620..17174)
                     /locus_tag="PKK36_03745"
     CDS             complement(16620..17174)
                     /locus_tag="PKK36_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93705.1"
                     /translation="MASRQSPVYVLRMDLFAHMAYSATVCSRTGLAGGARGSPRRWFA
                     DSTVWWAVGFGLLPDALSMGFPLLFFWLDGSAGNFFHEMDGDAMVSYRYAHSMISALL
                     FSGLIFLSVRRLFIPSLAWPLHLLMDMFTHGAGKYRTTLIYPVSDWGFDGISWWRHPW
                     LTGLIWLMLPVVWLCLRLARKKFQ"
     gene            17255..17629
                     /locus_tag="PKK36_03750"
     CDS             17255..17629
                     /locus_tag="PKK36_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNR93706.1"
                     /translation="MKPIKTPLAPPAIGPFSQAMQTGNFVFLSGQLGMNLETKALVPG
                     LEPQIEQLFKNMMAVLDAAGLGLKDIVKTTVFLSDMNNFATLNTIYARHFGDHKPARS
                     TVQVARLPIDALVEMECIAVRE"
     gene            complement(17619..18341)
                     /locus_tag="PKK36_03755"
     CDS             complement(17619..18341)
                     /locus_tag="PKK36_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNR93707.1"
                     /translation="MSRILIFLAAVNLAAGPVEYEINGRSYEGHYVSPSEGAPLVLLI
                     HDWDGVTDYEIRRANMLAEKGYAVFAADLFGKGVRPAEMADRRKLTGELYANREKMRA
                     LMIGALDAARAQGANADNAVAMGYCFGGSCVLELARSGSPLKGFVSFHGGLETPEGQD
                     YSQTKGKLLVLHGAADENITMDHFAALAKELEHAGVPNEMISYGGAPHAFTVFGSDRY
                     REDADKNSWVRFLQFLAGTLTP"
     gene            complement(18519..18800)
                     /locus_tag="PKK36_03760"
     CDS             complement(18519..18800)
                     /locus_tag="PKK36_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR93708.1"
                     /translation="MNPLMDIVYCVYVLRSLTDGKMYIGYSANLEQRLSDHFNGKVQS
                     TAPRRPLKLIHAEYFLSEDDARRREGYLKTTRGKRALKLMMRVSESDNE"
     gene            complement(19016..19091)
                     /locus_tag="PKK36_03765"
     tRNA            complement(19016..19091)
                     /locus_tag="PKK36_03765"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19055..19057),aa:Glu,seq:ctc)
     gene            complement(19149..20234)
                     /locus_tag="PKK36_03770"
     CDS             complement(19149..20234)
                     /locus_tag="PKK36_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNR93709.1"
                     /translation="MLNKSQTAAILVIILAAAAGALFVMKRTPSADFAYFQGNTMGTT
                     WSVRLAGPVPDSAALAVIQNDIELLLQNINREMSIYDPESIISEVNRLEADVAMNVPE
                     TLYGVMRFSLDLANLTGGAFDPTVGPLVNLWGFGPDGAVRASPGTDQIEAARSRVGFE
                     KLKLPESNVVLKTRADVYVDLGAVAKGFGVDAVAEMLVARGITSCLVEIGGEVRALGM
                     RKPGRKWRIGVQRPNYRAPSGSGLQGVLHLADMAAATSGDYQNFYVTDSGQTLSHIID
                     PRTAAPAGHSTAGVTVLAPDCMTADGWATALYVLGPEAGLPLVEAQPGLEALFVIREN
                     DQSFREMASSGFAALADYEPAGLETGK"
     gene            20317..21147
                     /locus_tag="PKK36_03775"
     CDS             20317..21147
                     /locus_tag="PKK36_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR93710.1"
                     /translation="MSENIPGIEITSLQNPRIKHVVKMRKRADRDEEGLLLVEGYREL
                     RRALDNGWRPEELFICEGLFLGSNEPALIQDCAAAGAPVIRCSEDVFRKIAYRDRPDG
                     LLAVGPQIHHTLAGLILSMNPFLLVAESIEKPGNLGTMLRSSDAAGVEAVIVCDRQTD
                     INNPNVVRASLGTIFSLPVVEASSEETLTWLRSKGIQILAATPSATMNYTEADCTRPT
                     AIVVGSEQYGLENIWMNGADAKVKLPMLGQCDSLNVATAATILLYEVVRQRRKHDEKG
                     "
BASE COUNT         4299 a         6535 c         6307 g         4136 t
ORIGIN      
        1 ggctgaaaac ctgtccgctg gatcagaaaa agttttttat ccgtgcggtg ctgaggcagg
       61 ggatcgaaga agtcgccgtt gagatcgttg ggcggcatga ccgtgtcgcg cgggtgcttc
      121 tgaacggcag ggtattctat cgtgaagaac cggggccggt cttcgacatc agcatgaatg
      181 aaatatgggc ggttgtagaa aaaagggatc cggcgctgga ggaaatcgcc ctcgatttca
      241 tcatgcggca ggtgcggggt gacaaaacgg ggaccgcaga tcagaacgtt cccgggctgg
      301 tgcgcgcgcg gatgctgggt tcggatgcgc cggtcatgac ggtcacgggc tccggcaatc
      361 agggaatctt catcggcgtg cctttttatg agctgtatca ggagatgggc aaagccatcc
      421 tcccggcggt gctgtttgcg ctgctgacgc agattcatct cacaaaaaag gcctcgcgca
      481 tttcaggcgc ctgcggactg gccaccaagg ccgctccggc cctgacggcg gggctgatgt
      541 atgcgcaggg ggcatcgctt ccggaaattt gcaaggcaat cgagaacctt cccagccgca
      601 tcggctatat tccctgcccc ggggcaatgg ccagttgcgg caccaaagcc cgcaaggtcc
      661 tcaagcatgt cctcccccag ctggaggccg tatgaaaaac attaagatta ccattgtggt
      721 tgaaaatacg gcttcgatgg agggcctgct tgcggaacac gggctttcag tatggattga
      781 aaccccgaac gcgaacatcc tgttcgatac aggccagggc ccggccctgc cgcaaaacgc
      841 cgccgcgctg aagattccgc tggagcgggc aaatgcgatc gtgctgagtc acggccacta
      901 tgaccacacg ggcggactgc cccatgcgct ggagcgcgca ccgaaggcaa aggttttcct
      961 gcatccggag gcgtttgcgc cacggttcag catccgcgac accatcatgc gcgccatcaa
     1021 catgccctat ccggctgaag gggctctgaa atcgcacagc gggaacatcg tgcacacggc
     1081 aaagtccatg gaggttgttc cgggggtatg ggtcaccggc gcaattccgc ggcgaaacaa
     1141 gtacgaagac acgggcggcc cgttcttcct tgataagctg ggtctgaagc ccgatcccat
     1201 ccccgatgat caggccttgt ggcttgatac cgcggacggg acggtcgttg ttctcggctg
     1261 cgcgcatgcc ggagtcgtca acacactgga atatatttca gaactcagcg gcgcttcgga
     1321 atatttcgcc gtgatgggcg gaatgcatct ggcgcgcgcc acggtcaagc ggctggggaa
     1381 aacggccgag gcccttgaac gcttccacgt gaagcagatt gccgccggcc actgcacggg
     1441 cgcaaacgcc atcgagtttc tggcccggcg cctgcccggc cgcatgattc cctgtgccgc
     1501 aggaagcgta tttgcctttg acggttccgc gcggcaaaac ggcccttgat tgcgccgggc
     1561 gtttcgctta cacatgccgg aaaacctccc gcccggctcg cgggcggcgc agcaggaaga
     1621 gagacagaat gaaaatttca aagcatgcgc ggcatcacta caggaaaaga aatcacgacg
     1681 atccgctgac cccctacggc aaccgcccga tggtgacccc catcgtgaac gcggtgaact
     1741 acgagtatct cgacttcgat gtcctgcaga agatcacgga cggaaagatt gacggctaca
     1801 cgtatcaccg cgacgacaat ccgacggtgc gcgcggtcga aaaacggatt gcggagctgg
     1861 agggcgcgga ggactgcgtc gtctgcacca cgggcatggc tgcctgctcc atggtttatc
     1921 tgacctatct gcgcgcagga gggcacctga ttctgttcca cgacatctac ggcgcgcact
     1981 acaaggtgtc gcttatcctt gaacgcctcg gggttgaagt cacctgggtg gatgccgcgg
     2041 actggaagag cgtcaaagac tgcatcaggc ccaacacgca gatgatattc ctcgagacgc
     2101 ccagcaatcc tctgtgcaag gtgatagata ttgcggccac aaagaaaatg gcccgcaagg
     2161 tcggcgcgat ggtcgtggtg gacaacacgt ttgcgacacc gtatcaccag cacccgcttg
     2221 agctgggcgc ggaccttgtt gttcacagcg cgaccaaggc tctgggcggg cacaacgacc
     2281 tcatggcggg cgccatcgcg ggctcgaagg aggattatga caagctgtgg ttcacgcggc
     2341 aggcgatcgg aacgaccctt gacgcctatt ccgcctcgct gctggagcgc ggactgaaaa
     2401 cctttccgct gcgggccgag cggatggcct ccagcgcgat ggccgtggca aagcatctgg
     2461 cgcagtgccg gggaatttcc ggcctgcact atccgggact gaagagcgat gacggccgca
     2521 gaatatccgc ccggcagatg gagcacggct tcggcggcat gctggcgttt gatgtgggga
     2581 aaacgcagga ggacgccaag cgattcatct ccgcgctgcg ggtcatacgc cacgcggtca
     2641 gcctgggctc cacagagtcg ctgatttgca taccgttcct gacgaccatg ctctacatgc
     2701 cgcctgagcg gcgcactctc ttcggggtca agcccaacac ggtgcggctc tctgtgggca
     2761 ttgagccggc ggcggagatt ctggccgaca tagaccaggc cctgaaaaag gtcgggaagc
     2821 gcaaatagcc ccggcgcggc aaaaagttcc aatcattgga agtttccgcg caaaaagttc
     2881 caaccattgg aagttttttc cgcaaaagtt ccagtcattg gaacttttgt atccggcttt
     2941 acagagggga cggacggtgt tagattgcag gagcaggaat cattcatatc tcgagcgcaa
     3001 tgaaaacagc ggtggacaaa aagttgctgg cggagcggat ggcctccttt cccgaactct
     3061 gcctgcgcgc ggggatccgg gtaacgcacc agcggacgga agtgtaccgc gaggtggcgg
     3121 ccacgctgga gcatcccgac gcggacagca tctaccggcg ggtgcgcaga cgcatccccc
     3181 agatctcgct cgacaccgtg taccggaccc tgggcctgtt tggggaaaag ggcatcatca
     3241 gccgcgtcag ctatccgggc gaccgggcac ggtttgacgg caacacgggg cagcaccacc
     3301 acttcatctg cacaaagtgc gggctgatcc gcgacttccg cagcgacgcg ctcgatgccg
     3361 tgcgtccccg gctgaagaac gtgccgtgcg ggcggatcac ctccgtgcag atcgagctgc
     3421 gcgggctgtg ccgccgctgc gcccgggcct gaaccgcctc gaagaactta tttccctttt
     3481 tcgcttgttt ttaggaatta ttactatata gtaataaaac attatcaaca gagcgggccg
     3541 cccgagcggg ggagcatcgg ccgggcggcc cgcgcaactt cagggagaaa tcagaatgac
     3601 agaacagaat gcagaagtcc gcatgccgct gatcggtgac aaggccccgt ccttcagggc
     3661 cgtcagcacc cagggggaaa ttgatttccc caaatggggc gagggcaagt gggtggtctt
     3721 tttctcgcat ccggccgatt tcacgccggt gtgcacaacg gaattcatga ccttcgcctc
     3781 gatggccgag gacttcagaa aacgcaactg cgagctgctc gggctttcca ttgacagcat
     3841 ctacgcgcac atcgcgtggc tgcggacgat caaggagaag atcgaataca acgggatgaa
     3901 ggaccttgag gtcaatttcc cggtgattga ggacctgaag atggacgtgg cacgggcttt
     3961 tggaatgctg cagcccggcg caagctccac acaggccgtt cgcgcggtgt tcctgattga
     4021 cccgaagggc gtggtccgca tgatcatgta ctatccgtca tcaaccggcc gcaacatgga
     4081 cgaggtgatg cgggtgctca tcgcgctgca gaccgcggac aagcacggca ttgcgacgcc
     4141 ggccaactgg cagccgggcg acgatgtgat cgttccgcct ccgggctcct gcggcaccgc
     4201 gaaagaccgc accgcaaagc ccgatccgga cgtgaagtgc ctggactggt tcatgtgcct
     4261 gaaaaagtgc ccgaaataat cagcgggttc tgatcatcaa acgccccgga gattcctccg
     4321 gggcgttttc atgactgacg gggagggttg ttccggctgc ttatttcgcg ggcccggcct
     4381 gctccagcat gcgtatcatt tcaggcgggc ccccgggagt cgcgaggctc agggcggttc
     4441 tgccgtaatt gtcttcaata tgagtgtctg cgcccctgtt cagcagcgct ttcacaattt
     4501 cgaggtttcc gatctttacg gcctccatta gcggcgtcac accaaaatta tcctgtatgt
     4561 tcacatccgc gccatgggcc agcagcgcgg ccgcggtttt ggcgtgaccc cggcttgaag
     4621 cggcgctcaa tgccgttctg ccgaaattgt tcttcgcatt aatgtcagcc ccgcgcgcaa
     4681 gcagcatttc agcaacttcg acatgctctc ctccgcgcgc ggcctcataa agcgcggttc
     4741 cgctttcgac atggcttttc ccggcggctt cattcacgtc tgcgccgtgg tccagcagga
     4801 tttttacaat gtccgtatgc cccctgtttg ccgccagcat aatggaggtt ttgccgttgg
     4861 cgtcctttgt gccgacatcc gcgccctgtt ccagcagcgc cttcactgca tcgatatacc
     4921 cgccccgtgc tgaaaacgga agcaccgggg aactcttttc gggaagatca acggccttgc
     4981 gcagcagtgt ctcgacaacc ccggcctgtc cgcgcgattc cgccgcctcc agcgcggcca
     5041 gcaggtccgc tgccgagctg cccgctccgg ccgcaagcag cgcttctgcc gtcttcggat
     5101 tcccggacaa cgcggccatc ttcagagccg tgcggttgtg agcatctgct gcgcgcacat
     5161 ctgctccatg cgaaagcaga acctctgccg ttttggcatg ccccctggcc gcggcgacat
     5221 tcagggcctt atcgagagat ccccccgccc cggcatccag cagaaactta accgtgctga
     5281 aatgatcacc ctcaaccgcc gccatcagca ccggttccaa attcacctca gcccctttgg
     5341 acaaaagcgc ctggaccgtt tcggtatgcc cgccgcgggc ggcggccgtc aatgccgccg
     5401 ccatgtccac cgaggccttc ctcgagatca atttctcaat tatccgggta tgcccctccg
     5461 ccgccgcaat cagcaccgcg ggatcaccgc tcatgcctct agcgttcaca tccgcgcctt
     5521 tggccagcag cgcctccacc gtttcgtcgt gcccgcccca gaccgccttt ataagcgcgg
     5581 tctctccatg agaagatttg gcattcacac gcgccccccg ggccagcagc gccttgacaa
     5641 cctcggtgtg tcctcctttt gccgcgaaca ttataggcgt ccaatccgta tcgtccttca
     5701 tgtttgcctt cgccccggcc gccaggagag cctgtacgat ttcggtgcgg cccttttcgg
     5761 ccgcatcaag cagcggggac cagccattca gggcgcttac attcaccttt gccccttttg
     5821 atatcagcag tttcacggtt tcggcatgac cgcggcgcac ggccaccatc agcggcgtcc
     5881 atccataaac atcactcgtg ttgacgcgag cgcctttgga cagcaaaaac tcagccaccg
     5941 cagtatgccc gctttcagcg gaagacatca gggccggaca gccatcttcg tcggttgcat
     6001 tcacatcagt gccctgcgcc agcagttccg caattcgttc catgcccgcg ggatctcccg
     6061 ccgcggcgat cagctgctga ttcagcgcct cgccgggccc gccattcgcc gcggccgcaa
     6121 gacccgccag aagaatcatc ccgcctgtca ctatgctgca ttttttaaac atgttcgaca
     6181 gcctttcact ggacatccgc ccgcccaaag catgccggcc ggatcccgtt tgtctgactt
     6241 aaatgggaga caacctctac cgatgaagta tatccaaatt atccacaaac acgcaattcc
     6301 cttgtgacac ggaattcggg ggctttcata tctggcacag gcttgtctgc gcccggggtt
     6361 tgctgtatgg tctttcccgt gcgcaaaaga gacacattcg atttcctgac aagcaccgcg
     6421 ggtgtgattg ccgacgccgc aggcgtgtat gccggcttca tgctcgccat gtggatccgg
     6481 tttttcagcg ggtggatccc actgcatggc gaaagcctgc cgcccatgtt ccagtatctc
     6541 gtcggcgcgg ccgtggccac ggttctgttt gtcctgattt tccgggccat ggatctctac
     6601 cgccggcccc agcacgggca cttcaccgag aaaatgccgc gcattgtgcg cggctgcgcc
     6661 tacggccttg tgatttcaac cgccatggcc ttcgccgtgc gcaatccgcc cttctcgcgc
     6721 gtggcgatga tcctttcgtt tttcacggtg acccttgtcg tgctgataga gcgaaatatc
     6781 cttttccagt gcgaacggca ctgggcccgg catcaggccg ccaaaaagaa cgtggtgatc
     6841 tgcggcatcg gcccgatcgc gggccagctc accgaaatcc tcagccgcga gcaccgcctg
     6901 cgcgcaaagc cggtcgccta tttttccgtg ggagatgaaa ccgccgatcc gcgcgttccg
     6961 gcggacctgg tccgcggccc gatgaacggg attccggatt ttctcggggc aaacgatgtg
     7021 gacgaggtga tcgtcgccaa tccctccggg cttacacacg cgcagctggt ggatctgatt
     7081 atccagtgcg aaaaggccct ggccgatttc cagatggtgc ccgatatttt cggagtgctg
     7141 accagccggg tggacatgca gatggtgggc ggcattccga tgcttggcgt aggcaagtgg
     7201 ccgctcgact atttctggaa ccgcgcccgg aagcgggcgg aggacatcgc cggcgccatc
     7261 gtcggcctga tcatctcggc gccgattatc gcaatcgccg cgctggtgat cagaaaaacc
     7321 tcgccgggcc cgattttcta ccgccaggag cgctgcggag agcggggaaa ggttttcaac
     7381 atctacaagc tccgcacaat gcactgcgac gcggaaagcc aaaccggtcc ggtctgggcc
     7441 agcccggacg atccgcgccg gacccggttc ggaacttttc ttcgcaaata caacctcgac
     7501 gaactgccgc agttctggaa cgtgctgacg ggcgaaatga gcctcgtggg cccccggcct
     7561 gagcgcccgc atttcgtgga gcagttcaag gaggacatcg cgcgctacat gtggcggcat
     7621 gtctccaagc ccggactgac cggctgggcg caggtcaacg gcctgcgcgg tcagacgagc
     7681 atcgaggacc gcatcagcta cgacctcttc tatctcgaaa actggtcgct ttcgctggac
     7741 ttcaagatcc tgatccggac gctgttcagc acgaagaacg cctactgacc ggggccgggc
     7801 cgccgcggcg gcgcgcgcgc ttccggttga cattcgggtc ctgatttcct atgttctgtc
     7861 actttatcaa ccggcgcaaa cgccggatgt tctggagaca gggatgccca catacgagta
     7921 tgagtgccag aaatgcggcc tgcgctttga actgtttcaa agcatgaagg acaagccgcg
     7981 tcagcgctgc cccacatgca ggggcaaagt ggtgcgctgc ctcggcaccg gcgcaggcat
     8041 cattttcaag gggtcgggct tctactccac cgattaccga agcccgacat atcatgcagc
     8101 ggcaaaaaaa gaaacggccg ccccgagtgc cggcggcgaa acaaaatcca gcggaaaggc
     8161 agaaaaaagc catgccacca atagttaaat gcaaaacctg cggaagtgag aacattctgg
     8221 gacagctttt ctgcaagcag tgcggcgcgc gcctcaagat gggcggaacg gtcgagcaga
     8281 agaagcccaa gggatcgggt ctccgcattg tgaagaccct tctgcgcatc gcggtgctgc
     8341 tggcctgcat tgcgctcgtc ggagggcttc tctggcccgc gcagccatca ggcgaattcg
     8401 ggtcggaacg ccatgccgac gtgtacgagg agaagatcgg ccgcctggag caggccgccc
     8461 gcgagggata cagcacggtc gaggaaatcg ccgaaacgca gatcaacggc tatttcgccc
     8521 ggctggtcac tcggaactcc ggcgaagggg attccgccag ccagctcggc atcggcgaaa
     8581 tcaatttcgc cttccggcag gataccgtca ttgtgtttgt cctggcccgg cgcggcccgc
     8641 tgaccctgac ctatgaactc acggcggcgc ccgtggcgga caaaggactg cagctcaacg
     8701 tgcagcaggt gcggctgggg cgcatccctc tgccgggctt cctgcatcag aggattctgc
     8761 agcgcatgtc cgtggtgttc agtcaaatgg atgcggaaca gctgattctc aggcatctgc
     8821 agaaaatcaa actggatgac aaacgcgcga tactgaccac ctgagcgtca caaggggata
     8881 tcatgagaga caaaaaagaa ttccacgcca ttctgacaca gatttcgggt ggactgtttc
     8941 gggaatacgg acggctggcc ggcgacttcg acttcaaccg ctatgtgctg aaaattcatt
     9001 ttgtgcagga cgcagccgaa gacggcccca ccctgtttgt ggtgcgcgtg ccccagttta
     9061 tttcagagtt tccggaacgg ctgttcagca gcgcggtgcg ccgcacggcg ctggaagatc
     9121 tgctggttcg caaaatcagt gaaagcatcg agggcatggc gcacttcaat tccagcggaa
     9181 cagcgcgccg acgctttcag atcgcccggc tcgggcagaa aattcttccc cggtcctcgg
     9241 tcatcgtgac cgatgaatat gtcgaagccc gcctgtatgc cagcctcccg cgcgagggcg
     9301 aggtgattcc cggagatgcc gtccttgaac tttttttcga ggatctgccg gccattgtga
     9361 ataccgcgct gatctactgc aacctcgacc gcgacgaagt ggagcacacc gtgggcctga
     9421 tggaggacac agatgtcatc cgccagatgc ttcccacgca gggcatggtc ggcttcgtcg
     9481 gcgacggcct tcttctttcg cgcaaacccg ggaccgacct gcccgatttc accaggaaca
     9541 ccagcctgaa ggttgacgtc tcggcgcggc tcgaagtcga ggtcccccat ctcagcaagg
     9601 tgtatggcgc cggcatcccg gcgggcatca ccgtgatcat gggcgacgcc tattccgggc
     9661 gcattgagat catgcaggcg ctcgcttccg gcatctacaa ccatatcgcc ggtgacggcc
     9721 gcgaactggc catcaccgtg cccgacgccg tagagatacg gtccgaaccc ggccggagca
     9781 tcgcgcaggt gaatgtcagc gcatttgtca gcggccatgc ctgcggttgt gcgcccgagg
     9841 cctgctcgac gaattcggcc gacgccttcc tgtcgcaggc cgcggccacg gtggaagcac
     9901 tgcaggccgg agcacgcgtc ctgctgtttg acgaggccac atcctgttct gcttttcttt
     9961 cttccgatca gcggctgacg gccctgctgg gggcgcgcag cggaatcatt cctctggcgg
    10021 cccgggcgcg gcagctcgcg gacgatctgg gtgtttcact ggtcattggc ggggaaacct
    10081 gcgtggcgga attcatcccc attgccgacc gggtgctgcg cgtgaacagg aatgtcatct
    10141 cggacatcac cgaggaggcc cggaaggcca cggtggcaca gccgccccca cgcgcggccg
    10201 ccattgacgt ggccgcgctg gcggaacagc agcggtggat tgtgcccagc agcattgatc
    10261 cgaccctcgg gcgcgaagac tcctacatat gcgctccgaa tgtgcacacg ctgtgcttcg
    10321 ggcgaagcac aatagacctc tccgccgtat cgcagctggc agacattcag cagaccgaaa
    10381 ccatcgccca cattctctat tacgcgaagc ttcgctacat ggacgagagc cacccgctgc
    10441 gggagattct cgacctggtg gaccgtgatc tcagcaccga gggcctggaa tgtctcgcgc
    10501 gcgagctgcg tggagatctg gcccgcccgc ggcgctacga gatcgcggcc gccctcaacc
    10561 gcctgagcac gctgcgggtg cagaacccga ccgactgacc cctcgaatga aactggtgat
    10621 ccagcgcgtc agccgggctc gcgtgggcgt ggaggagaaa accgtttcgg aaatcggccc
    10681 gggggctttg attcttctcg gcgtggccag gggcgacacc ccggccgatg ccgaatggct
    10741 ggcgggcaaa gtctcgcggc tgcgcattta cgacgacccg cgcggacggc tcaaccattc
    10801 cattgccgaa acaggcggat cgtatctggt cgtcagtcag ttcaccctgt tcggcgactg
    10861 ctccaagggc aaccggccca gctatattca ggccgccgat ccggacgatg cggccgcgct
    10921 gtatgatctg tttgtggaaa aactgcgcgc actcgggcac acggtccgga ccggagtgtt
    10981 tcgcgagcac atggatgtcg agcttgtcaa cgacgggccc gtgaccctgc tgatggagag
    11041 ctgtgggcgc tgaacggaac atcctgcgac tggttctatc cgccctgctc tgcggggcgg
    11101 cagcccgcct gcccgcccag gaaacccggg accgccgcgt cgtcagcgcc accggccgtt
    11161 tcgtggcgct cagcccggcc ggacaggacg ccacctatct ggtggtctgg gccgaagatg
    11221 tggttgaacg cctccagaag ctgctgggca tggcactgcc ggtgccgcgc ggcgaggccc
    11281 tttatctgat tgtaggtccc ggcgagggcg ccatttccgc cgaacagtac tatgacgagt
    11341 tcggtctgcg ccagaaactc catatcagcg gcatcgaagc cgccgaccag gagcaggtgc
    11401 tggaacacct gtgccggttt ctgctgaacc ggcagacgct tccggaacag gttcccttct
    11461 ggttcgccgc cggggccgcc cagaatctct ttcccgaagc ccgtgttcgc aacatggaaa
    11521 cagcgatatc cgaatggaga gcggaccggg ccccggactt ttcggacatc accggcggcg
    11581 actggccgga cacccccgat ttccggacaa gggcttttgc cggcctggct gttgcggctt
    11641 tgcttgaatg gttccccgac tccgcccgca cagagcagtt cctctccgcg tgcgccgcag
    11701 ggcagacgcc ggcggcggaa ctggccgcac agctgaccgg aagggggtcg gtgcgggatc
    11761 tggaggcagc gtgggatgtc tggctggcct cgcgccagaa catccggatt gtgggggccg
    11821 gatcgccgcg gcaggatgca gagcgcctgc gggccatgct ggaaatcacg cccgtgatga
    11881 ttcaactcgt ggccggccgg aaggtgccgg gagaattttc agctcaacgg atgattgccg
    11941 aacgcgcgga ggactgggtt cccgccgtag cgcgccatct cgacctcaaa ctggaaagct
    12001 tcagtctggg atgcgcgccg gaaacacaag gggtcatccg ggcctggcgc gatttcctgc
    12061 agaccctctc cgtgcgagat gaaaagcggc cctggtattc actgcgcaaa gcgccggcgg
    12121 tgtccgagcg ggagctgctc gtcaaactgg cgcgggccga gcagcttctg tttgagctgg
    12181 ccgaagccgc ttcggacgaa tagccgcatc tatttttcga cggcatgcag ctcttcgctg
    12241 cggtcccggc tcaaatcagc cgccgcgaag gattccgcgt cctgcgggag ctgaagatga
    12301 aaccggctga gcagatccac ctcgccccgt ccctctgcaa gttcaaagcc gagaatatgg
    12361 ccgccctgtg tccggtcccg gcttataaaa tgcagatgga agccgggaac attgacccct
    12421 ttcacgaatt cggggcagcg gaaaccgacg acatcccctt cgatatcctc cataaagaac
    12481 tccggctgcc tggcggtcac ctgtttcagg gggggatagg gtttctgctg gcggggcacg
    12541 ctgcgcgtat gcatggattt aaacttcccg ctgatccgaa ccgcgcagaa gaggttctgg
    12601 tttggcgcaa tccggttcac ctgttcctcg atggccgcaa aatccgaggc gggcgtcagg
    12661 tcaaaccgga tatccggatt gaagaagcac accgacgcaa agggtgtcga agcatcatgg
    12721 ttcacttcaa ccacggagcc gtcggccttc acctgataga tgcggccgtc gaggaccacc
    12781 atctccccgt ccagggcttc aaacgtgccg atgccgaaat tgccctgccg gagcagatcc
    12841 ctgccggaca gatgcccgtc ataaacgcca acaaggaggg catcgatcgt ggacatttga
    12901 aaaacggtgt tatcgggcgt ggtggcgcag cctgcggcga gcagggccag cgcggcggca
    12961 agaagcagat ttttcatcaa gcacctcatt tttcagacat gcctgatgct ggaatggcgg
    13021 gcggagaaaa tcaagaacaa cctgacaggg gattagtttc tgcacttttt gcttgtccgg
    13081 ggcgggcggc cgttctatct tgcggccaga aatatggcgt ggtttcgcca agtaaagcgc
    13141 ttcgacgggc tggttccggc ggggcaatga cagctctggt tcttaacagc gggtgagaat
    13201 aaaatgaata tgatcaaacg actgtactgc cttattccca tttctttttt catggcaaac
    13261 tccgccttcg cggaaaccgc cccggctggc gcgcgccaga ttcccggctg gtggtatgtc
    13321 tgcccggtcg ccagcgtggt tgccctgctg atggcgtttt tcttctatcg cttcatcatg
    13381 aaatcctcca gcggaaccga ccgcatgcag gaaatcgccg gctatgtccg cgaaggcgcc
    13441 tatgcctatc tccgccgcca gtacaaggtg gtcggactgg tctttgtggt gctggttgcg
    13501 gtcctttcct tcatggcttt cggtctcgga gtccagcacc gccttgttcc ctttgccttc
    13561 ctgaccggcg gccttttctc cggactggcc ggcttcatcg gcatgaaaac tgccacggcg
    13621 gcctctgttc gcaccgcgca tggcgccagc gaatccctga acaagggcct cacaattgcc
    13681 ttccgcgccg ggtcggtgat gggactgacg gtggtgggcc tggcgcttct cgacatctcc
    13741 ctctggttcc tctatctcta caagtaccac cacattgtga ccggcggtca cgagatgaac
    13801 ctcgaaacca tcaccgttgt gatgctgtgc ttcggcatgg gcgcttcgac ccaggcgctg
    13861 tttgcgcgcg tcggcggagg aatctacacc aaggccgccg atgtcggggc tgacctcgtg
    13921 ggcaaggtgg aagccggcat tcccgaagac gatccccgca atccggccac catcgccgac
    13981 aacgtcggcg acaatgtggg cgatgtcgcg gggatgggcg ccgatcttta cgaaagctat
    14041 gccggctccg tgctggccac agccgcgctc ggcgtggcca tggccagcgt ccatgggttt
    14101 ggagtccaga aagcctttat ctatctggcc gccccgatgc tgctggccgc catgggcatc
    14161 ctgttctcga tcatcggcat ctttgtcgtg aaaacccgcg atgacgcaaa catgaagcag
    14221 ctcctttcgg cactcaaccg ggggatttac ctttccagcg tcctgatcat tgccggggcc
    14281 ttcgcgctcg tgcgggtgat gggactcccg accggcgtgt tcaccgccat caccatcggc
    14341 gtggtgctgg gtattgtgat cggcaagggc acggaatggg ccacggcagc agaatacaag
    14401 cccacgcgct acgtcgcgga ccagtcgctg accggaccgg ccaccgtgat catcggcggc
    14461 atcgccgagg gaatgatgtc cacatggatc cccattctgg ccgtctgcat cggcacgctc
    14521 tgctcctttg cggtcatgga cgggtttaat gacatccttg cggggcttta cggcgtgggc
    14581 attgcggcgg tcggcatgct gtccacgctg ggcctgactc tcgccaccga cgcctacgga
    14641 ccgattgccg acaacgccgg cggcaacgcc gagatgagcg gcctgcccaa ggaagttcgc
    14701 cagcgcacgg acgcgctgga ttcgctgggc aacaccacgg cggccatcgg caagggtttt
    14761 gcgatcggct ccgccgcgct gaccgcgctg gcgcttctgg caagctatat cgaagaggtg
    14821 cgcgtcggca tccatcgcga ggccgccatt gaatatgccg cgccggccgc cgaagaaagc
    14881 tatacggtgg tgaacgtcgg cgccgccgac aacgggaagt atgccatctg cctgccgggg
    14941 gccgcggccg ggagccgcca gtcccagcag ttctacacgc tcagcgcgtt tgagggaacc
    15001 ggcgaaagag ccgtgcgcgt cagcggcccc gacgggcagc cggtcagcct gatcgtcaat
    15061 gaagtgggcg agatggtgaa ggaccgcggg gcggcggatc tggacggacg gctcctctgc
    15121 gcccaaaaag ccggactcat ggattatatg aacttctaca acgtgacgct gatgaacccc
    15181 aaggtgctgg tgggtgaatt catcggcgtg ctgctggtgt tcgtcttcag cgcgctcacc
    15241 atgaaggccg tggggcgcgc cgccttcgcc atggtcaccg aggtgcgccg gcagttcaag
    15301 gagtctccgg gcatcatggc cggcacagaa cgtccggatt atgcccggtg cgtggccatt
    15361 tccacggccg gcgcccagcg tgaaatgatt gtcccctccc tgctggccat caccattccc
    15421 gtgctcgtcg gcctgttcct caatgtgtcc ggcgtcgtgg ggctgctggt cggaggtctg
    15481 tgcgccggat tcgccatggc ggtctatatg gccaacgccg gcggcgcgtg ggacaacgcc
    15541 aagaaattca tcgaggccgg aaaccacggc gggaagggaa gccttgcgca caaggccgct
    15601 gtcgtcggcg acacggtggg cgatccgttc aaggacacct gcgggccgtc gctcaacatc
    15661 ctgatcaagc tgatgagcat ggtcagcatt gtgtttgcgg gactgatcgt caaatacgcg
    15721 cccgcgatcg gcgcctggct cggaatcgga aactgatcaa ggaaggataa ggctatggac
    15781 attcatctgt atctcgtctg ctaccagagc gaggcgctcg tggcctctca cctcgatgcc
    15841 gcggcattcg gcgcccacct cgcggtgggc acccagaaac ataccagcgg caacgtcatg
    15901 tttgtggaaa ttgatccttc gttcaagagc gacgcgctga agctgaacaa gctgtcatcg
    15961 cggtgcattc ctcatgcgga cggctcgccg cgcagaagca agtacatcag catctaccgc
    16021 gcgctcgaac acattcccct gtccgccttc ggaaaacttt acatgaccac ccgcgacgga
    16081 cgggtgctgg gcctggacag caaaccctac gacccggaat ccgaggaacc cggcacgaac
    16141 ctctacaccg agctgtgccc cgtcttcccc cgcgtggcct catcgctccc gcccttccgg
    16201 ttctgccggt tcatcacaaa tcccgacaac ccgctgcatg tgccgcggat catcttctcc
    16261 gacaacctgc tcgaccggga agacgacgga agccttgccg gctacctgcc ctatcccaac
    16321 cgcgagcaca tcgtggattg cattcgcgca ctgacatcca gcgagggcaa gcccacgaaa
    16381 accatcgaga gaaatccgca gctcatcgcc ttctaccgaa ccgtgggacg cggattcttc
    16441 gccggcgacc agaccgacat gatattctat ccgttcccgt cccgtgcgga acttgaaggg
    16501 gaacaccatc tctggtggcg ctccgcctcg atggattgag cggaccagaa gttccagtca
    16561 ctggaagttt cgcccggaaa agttccaatg attggaagtt ttttcgcaaa aagttccagt
    16621 cattggaact ttttacgggc caggcgcaga cacagccaga ccacgggcag catcagccag
    16681 atcaggcccg tcagccacgg atgccgccac cacgatatgc cgtcgaaacc ccagtctgaa
    16741 acgggataaa tcagcgtggt ccgatacttt cctgccccgt gcgtgaacat gtccatcagc
    16801 aggtgcagcg gccacgccag cgaaggaatg aacagccgtc ggacggaaag aaaaatcagc
    16861 ccggaaaaca gcagggcgga aatcatgctg tgggcatagc ggtaagacac catcgcgtcg
    16921 ccgtccatct cgtggaagaa gtttccggca gatccgtcca gccagaagaa cagcagcggg
    16981 aaacccatcg aaagagcatc cggaagcagc ccaaagccga cggcccacca caccgtggaa
    17041 tccgcaaacc agcggcgcgg actgcccctt gcgcctccgg ccaggccggt gcggctgcag
    17101 actgtcgccg aataggccat atgcgcaaac aaatccatgc gcagaacata caccggcgac
    17161 tggcggcttg ccaccgtttt gtcccgcgcg cttgacttcc ccgctcccat tcggcaattt
    17221 gacggaggaa atcagccatc agaaaggaca tgcaatgaaa ccgattaaaa ccccgctcgc
    17281 gccgcccgcc attggaccgt tttcccaggc catgcagacc ggaaactttg ttttcctgtc
    17341 gggacagctt gggatgaact tggaaaccaa agcgctcgtg ccgggcctcg agccgcagat
    17401 tgaacagctc ttcaaaaaca tgatggccgt gctcgatgcc gcggggctcg ggctcaaaga
    17461 catcgtgaag accacggtgt tcctgtccga catgaacaac ttcgcgacgc tcaacaccat
    17521 ctatgcgcgg cacttcggcg accacaagcc cgcccgctca accgttcagg tggcccggct
    17581 tccgatcgac gccctcgtgg aaatggaatg cattgcggtc agggagtgag cgttcctgcg
    17641 aggaattgca aaaaccgcac ccacgagttt ttgtcggcgt cttcccgata acggtcggaa
    17701 ccgaaaacgg tgaatgcgtg cggggctccg ccgtatgaaa tcatttcatt gggcaccccg
    17761 gcatgctcca gctctttcgc aagcgctgcg aaatgatcca ttgtgatgtt ttcatcggcc
    17821 gcgccgtgga gaaccagcaa tttgcctttg gtctgcgaat aatcctgacc ctcgggtgtt
    17881 tccagcccgc cgtggaaaga aacaaacccc ttgagcggcg agcccgaccg cgccagttcc
    17941 agcacacagc ttccgccgaa gcagtagccc atggccacgg cattgtccgc gttcgcgccc
    18001 tgcgcgcgcg ccgcatccag cgccccgatc atgagcgcgc gcattttctc acggtttgcg
    18061 taaagttcac ccgtcagctt tcggcggtca gccatttccg cgggccgcac ccccttgcca
    18121 aaaaggtccg ccgcaaaaac cgcatatcct ttttcggcca gcatgtttgc gcgcctgatt
    18181 tcataatccg tcaccccgtc ccagtcgtgg atcaggagca ccaggggcgc cccttcggac
    18241 ggactgacat agtggccttc gtaagatcgg ccgttgattt catattcgac cgggcccgcc
    18301 gcaagattca cagcggccag aaatatcagt attcgagaca tggccttcat tttttactgt
    18361 tcctttcttc gaacaggata cttcatgaat gatgtatcat caaacctcga cgggaattcg
    18421 cagtgcagga ctcatgaacc agagcaaaac acagttcatg gcccgccatg aaccgtgccc
    18481 ggagcgaagc gaatggctct ggttttagca acgtccactt attcgttgtc tgattccgaa
    18541 accctcatca tcaatttcag ggctcttttc cctctggtgg ttttcaaata tccttcgcgc
    18601 cgccgggcat catcttctga gagaaagtat tcagcatgta taagtttcaa aggccggcgc
    18661 ggtgctgtgg actggacctt tccgttgaaa tgatcagaca acctctgttc caaattggcg
    18721 gaatagccaa tgtacatctt gccgtcagtt agacttctga gaacatagac gcagtaaaca
    18781 atatccatca gcggattcat ttaacagtct cctgtaaaag gatggtcaga aatcatgccc
    18841 tgcaccagaa ttttcctccc ttcgttcgga aaacacggtg cagaactcat gaaccagagc
    18901 tgggtggttt tgtgacattg gctggttatt cgacggcccg ccatgaacca gagcaaagca
    18961 cagttcatgg cccgccatga accgtgcccg gagcgaagcg aagggctctg gttcatggca
    19021 cccccaaccg gattcgaacc ggtgttaccg agatgagaac ccggtgtcct aggcctctag
    19081 acgatggggg cgctagaagt gaaaagaatc tgccgaattg ttttcttttt cgaaagaaaa
    19141 taattccatt atttgccggt ttcaaggccg gccggttcat aatccgcaag ggctgcgaag
    19201 ccggaactgg ccatttcccg gaatgactga tcgttttccc gaattacaaa gagggcttcg
    19261 aggccgggct gcgcttcgac aagaggcagg ccggcttcgg gtccgagaac ataaagcgcc
    19321 gtcgcccatc cgtcggccgt catgcagtcc ggggccagca cggtaacgcc ggccgtgctg
    19381 tggccggcgg gtgcagccgt gcggggatca atgatatgcg agagcgtctg ccccgaatcc
    19441 gtcacgtaga aattctgata gtcgcccgat gtggcggcgg ccatgtccgc aagatggaga
    19501 accccctgca gcccgcttcc ggacggggcc ctgtagttcg ggcgctgcac gccgatgcgc
    19561 catttgcggc ccggctttct catgcccagc gcgcgcactt ccccgccgat ctcaaccagg
    19621 cagctggtga tgccccgcgc gaccagcatt tccgccacgg catccacgcc gaaacccttg
    19681 gcgaccgccc cgagatccac ataaacatca gcccgggttt tcagcacgac gtttgattcc
    19741 ggcagcttaa gtttttcaaa tccaacccgc gagcgggcgg cttcgatctg atccgtgccg
    19801 ggagaggctc gaaccgcgcc atcgggacca aagccccaga ggttgaccag tggacccacc
    19861 gtcgggtcga acgcgccgcc ggtcagattc gcgaggtcca gcgaaaaacg catcacgccg
    19921 tacagggttt ccggaacgtt catcgcaaca tccgcttcaa gccggttcac ttctgaaata
    19981 atgctttccg gatcgtagat ggacatttcg cgattgatgt tttgaaggag cagttcgatg
    20041 tcgttttgga tgacggcgag cgccgcggaa tcgggaaccg ggcccgccag ccgcaccgac
    20101 caggtggtgc ccatggtgtt cccctgaaaa tatgcgaagt cggcggacgg agtccttttc
    20161 attacgaaaa gagcgcctgc ggctgccgca aggataataa ccagaatggc ggccgtctgt
    20221 gatttgttca gcatgccgcc agagatagcg caacggcggg caaatgcaaa gaaattctgc
    20281 tggcatgagg acgcagatcg cctatcatcg cgcatcatga gtgagaacat cccaggaatt
    20341 gaaatcacca gcctgcagaa tccgcgcatc aagcatgtcg tgaagatgcg caagcgcgcg
    20401 gaccgcgacg aggagggact gcttctggtg gagggctacc gcgaactgcg ccgggcgctc
    20461 gacaacggat ggcggcctga agaactgttc atctgtgaag ggctttttct gggaagcaac
    20521 gaaccggcgc tgattcagga ttgcgctgct gcaggcgcgc cggtaatccg gtgcagcgaa
    20581 gatgtgttcc gcaaaatcgc ctatcgcgac cggcccgacg ggctgctggc ggtcggcccg
    20641 cagattcatc acacgcttgc cgggctcata ctctcaatga atccattcct gctggtggcc
    20701 gaatccatcg agaaaccggg caatctggga accatgctgc gatcgtctga tgcggcgggt
    20761 gtggaagcgg tcatcgtctg cgaccggcag accgacatca acaatcccaa cgtcgttcgc
    20821 gccagcctcg gaaccatctt cagcctgccg gtggtggagg cctcatcgga ggaaactttg
    20881 acatggctgc gcagcaaagg aatccagatt ctggcggcca cgccgtcggc aacgatgaac
    20941 tacacggaag ccgattgcac cagacccacg gccatcgtgg tgggttcgga gcagtacggt
    21001 ctggaaaaca tctggatgaa cggcgccgac gccaaagtga aacttccaat gcttggacaa
    21061 tgcgactcgc tcaacgtcgc gaccgcggcg accatccttc tgtatgaagt ggtccgccag
    21121 cgccggaagc atgacgaaaa aggctgaaaa cagccaaaaa acgggcattt tttgaaaaaa
    21181 tcaagcaaaa ttggccattt ttaggcgatt tttggcgttt tttcactact gtccggtaaa
    21241 aatattcaaa ggataaacag catctgaata gcctcat
//
LOCUS       DAOQWR010000028        21077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2882_length_21077_cov_5.604700, whole genome shotgun sequence.
ACCESSION   DAOQWR010000028 DAOQWR010000000
VERSION     DAOQWR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21077
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_21077_cov_5.604700"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK36_03780"
     CDS             <1..303
                     /locus_tag="PKK36_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93711.1"
                     /translation="PEDAIKLAVEMGCDTMFVLTDDPPYIKRGDAETGVEIPEHQDNI
                     LDFAKNIESKYGRQVKINTIAYKPWSNAKGQRAIDFLKNLSRSTGGRFKHIKRKDE"
     gene            296..967
                     /locus_tag="PKK36_03785"
     CDS             296..967
                     /locus_tag="PKK36_03785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR93712.1"
                     /translation="MNSEVPESQIQEDKPSGRAGFDYRMTHHEINDLDLSAYDGPVML
                     VNSPERLPDAVAALSADRILGFDTETRPSFTKGTNYKPALLQLAGEQVVCIFQLKLIG
                     LPRPLIDILANPEVIKAGVAPQRDALELKALAEFEPAGMFDVSEAGRMGGIQNLGLKG
                     LAAVLLGERISKRAQLTNWARQKLSPHQITYAATDAWISRRIYLRLQEMDMLRQPVRK
                     MEKES"
     gene            964..1140
                     /locus_tag="PKK36_03790"
     CDS             964..1140
                     /locus_tag="PKK36_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93713.1"
                     /translation="MNKRIIIALLLIALVAIVLILSSGTTTVNLGFGRMHMHTPLALL
                     LFTSIGVVVGLLLK"
     gene            1205..2434
                     /locus_tag="PKK36_03795"
     CDS             1205..2434
                     /locus_tag="PKK36_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93714.1"
                     /translation="MMTVPPHVRKLMEQGVLIPAPASVEIDERINPLLIAPGVVLHTG
                     TKLLGEHTSIGPGCVLGEEGPVMLKNCQLSSGVKLQGGSFEGSVFLGRSSMGPCAHVR
                     AGTLLEEEAGGAHSVGFKQTIFLPYVTAGSLINFCDAMMAGGRSRKEHSEIGSSFIHF
                     NYTPHRDKATPSLIGDVPRGVMLNNDPIFLGGQGGLVGPVRIDYGCVTAAGTVYRRDA
                     LEPNMLLTAKSRGVGHPVPYKSGAYRGIARIVANNLIYTGNLWALTQWYRFVRRHFME
                     RNPFDHACYNGAIRLLEDARRERIKRLAELAKNLKESIAILSAKGDGPELAAQHAFVK
                     AWPGIEEAVRGGPEPDFSVDQLQDVFSAAWDRVSGSEKDYLKAIKNLAPDEARAGTAW
                     LQSVVNQIACLFSNAES"
     gene            2439..3860
                     /gene="gatB"
                     /locus_tag="PKK36_03800"
     CDS             2439..3860
                     /gene="gatB"
                     /locus_tag="PKK36_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNR93715.1"
                     /translation="MNYKVTIGLETHVQLKTQSKIFCGCRKDFGDEPNTHVCPVCLGY
                     PGAMPVLNEEAVRLTVMTGLMIGCKINSYSKFDRKSYFYPDMPKNYQISQYDQPLCLG
                     GSVEIETEAGKRSIEVTRIHLEEDVAKNIHFENFSGVDFNRAGTPLMEIVTEPVIESA
                     DEAFAYLQALKQILVYGGISDCNLEEGNMRCDVNCSVRPEGQEKLGVKTELKNMNTFK
                     GVHRALKAEIARQISVLEKGGTIQQETRRWDDVAGVTLAMRSKEYAHDYRYFPEPDLM
                     PVVLDEKTVEEWRCRLPELPAKRRERMTAEYGIPEYDAGVLAADKHVADYFEETAKLC
                     GSGKAASNWIMTEVMRVLSAQEIEIRGLKITPVALAALIKLVDAKTISSTGAKEVFAV
                     MLEEGGDPAAIVKARGLAQVSDTGALEAMVMQAIAENEKSVTAYRAGKEAALQHLVGQ
                     VMKLSRGKANPGVVIELLKKNLA"
     gene            3857..4846
                     /locus_tag="PKK36_03805"
     CDS             3857..4846
                     /locus_tag="PKK36_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNR93716.1"
                     /translation="MNKIPAKDGQKEPDPLLAAFVDYLEGEKNASHHTVTNYLLDIKQ
                     FIRFSWGNDAKPPYGWKQCDRYAGRKFLVHLMKEGRNPTTVLRKLSSMRSFFRFMLRE
                     EMVTFNPFSGVAMPKKGKYLPEVLSVAEVNRLLDAPKLVAAKQGAPQSPGDKAWMDYA
                     AARDRAILEVLYSTGMRVSELAGLTPGQVDFLAGIIKVRGKGKKERLCPLGGPASRSL
                     REAVDKRDMFCVSIGEKPGSAGFFLNRKGGRITTRSIERMMKKYLQAANLNPEHSPHV
                     LRHSFATHLLDNGADLRSVQELLGHASLSTTQIYTHITVERLKEVYKQAHPRA"
     gene            4862..6169
                     /locus_tag="PKK36_03810"
     CDS             4862..6169
                     /locus_tag="PKK36_03810"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNR93717.1"
                     /translation="MIWIVYNILFAVGFTLMLPHFIYRMVRRGGYARNFVQRLGFYSR
                     SIRTKLSAGRHIWIQAVSVGEIFVAFRFMEEIRRRNPDARFVLSTNTSTGYALARSRI
                     GESDILIYFPVDFPLVMRRVLGLINPAALILVECELWPNLIRLAKRRGTPVLLVNGRI
                     SEHSFRGYRKLKFFTKKILPMVDLLCAQSAADARRLISLGAPERNVKVMGSAKYEVAS
                     KDPTGEEKAAAILKAAGIDITAPILLGGSTWAGEEKILLNAFKILRDEFKNLQLVLAP
                     RHAERAGEVIHEIESAGCRAVQRSKLDLSAARVWEPDDVLLVDSTGELKNFYPYATVI
                     FIGKSLTQHGGQNPIEPAVYGKPVIVGPNMENFPVVMEDFREASALIQVADETGLHSS
                     IRALLSDAQLRGDYGGRAASLVEAKSGALAETVELIIPHLLKS"
     gene            6235..7101
                     /locus_tag="PKK36_03815"
     CDS             6235..7101
                     /locus_tag="PKK36_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93718.1"
                     /translation="MKALIPLMLCMLTVGAGFADTTVDASNPYAYGANIGWINARGDI
                     ASGAVFGQSYCTGYLYSANCGWISLGNGPTNGWRYSNTAADDWGVNHDGTGSLTGFAY
                     GANIGWIAFEQTYGQPRVDLLTGNLSGYVWGANVGWISLSNAQAYVQTTRLDSGPDSD
                     ADGIPDFWEMKQVGDLATLSGAGHDEDDDGASDTDEYSSDTDPTDINSSLEITALERL
                     DGTNLVTWSIEPTRFYKLEHASSASNNADWGDSGHGIMSPELADEMTRELADSAQTSQ
                     FFRARAIVPLSP"
     gene            7317..8948
                     /gene="pgi"
                     /locus_tag="PKK36_03820"
     CDS             7317..8948
                     /gene="pgi"
                     /locus_tag="PKK36_03820"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007470796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNR93719.1"
                     /translation="MHKRISAARSPEWKALQAHQKEIKSQHLRDLFQQHPERAETFSL
                     EACDLFLDYSKNRITFETMPLLLKLTEVARLRDEIRAMFSGEKINATENRAVLHAALR
                     NVSNRPIMVDGKNVMPDVRRVLDQMRDFSTRIRSGEWKGFTGKKIRHVVNIGIGGSDL
                     GPVMASEALKAYSDRDITLHFVSNIDGTHLAETLRKLNADETLFIVASKTFTTQETMT
                     NAHSARGWCLASLKDPAAIAKHFVALSTNTEAVKSFGIDPANMFEFWNWVGGRYSLCS
                     AIGLPLMISIGPDHFMEMLRGFHAMDCHFESAPFEQNMPVILGLLGIWYNNFFDAETH
                     AILPYDQYLHRFAAYFQQGDMESNGKYVDRNGSPVKYQTGPVIWGEPGTNGQHAFYQL
                     IHQGTKLIPADFIGFVQSLNPIGDHHDKLMANFFAQTEALAFGKTADEVRAEGTRRAL
                     VPHRVFQGNRPTNTIMASKLTPAVLGQLIALYEHKIFTQGIIWNIYSFDQWGVELGKV
                     LATKILKELQDPASAPVHDSSTSRLISRYRAERKA"
     gene            8950..9822
                     /gene="lipA"
                     /locus_tag="PKK36_03825"
     CDS             8950..9822
                     /gene="lipA"
                     /locus_tag="PKK36_03825"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNR93720.1"
                     /translation="MQQCPPKRLPAWMRTSRRTDRSFVRVQRLLEAESLCTVCQDAKC
                     PNRLQCWNEGTATFMILGHACTRNCRFCAVQKTVPREPDPNEPASVARAVKAMNLRHA
                     VITSVTRDDLPDGGAGHFAGTVRMIRAAGVTVEVLVPDFNGCAAALETVLDARPDVFG
                     HNIETVRRLQAVVRPMASYERSLAVLRAAAAHGGALVKSGLMLGMGETRAEILQAMDD
                     LLEAGCELLTMGQYLAPTPDHYPAASYLAPEEFDELGDAARAKGFRAVASGPLVRSSY
                     RAAQMFVQAQGAKP"
     gene            9819..10514
                     /locus_tag="PKK36_03830"
     CDS             9819..10514
                     /locus_tag="PKK36_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93721.1"
                     /translation="MNPLPFKPRYCVLIPAYQEEKNIASVVRDALKYCPQVIVVDDGS
                     GDKTASVADQAGAIVHRHETNRGKGAALQTGFAVARARECDAVITMDADGQHKPEEIP
                     RFMDAYERTRIPVLVGNRMADPAGMPLIRRLTNRFMSCMLSRVMKQYVPDTQCGFRLF
                     ASDLTPFVPTESAGFAAESEILLYVARRGIRMDSVRVSTVYTGAEQSSIRPFSDAFRF
                     FGMLWRNRERKRF"
     gene            10514..11173
                     /locus_tag="PKK36_03835"
     CDS             10514..11173
                     /locus_tag="PKK36_03835"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNR93722.1"
                     /translation="MQESAAAHLRREHIEVFIRGRGVCDKRVLRALQDVPRELFAPGM
                     SLNDVYGDHPLPIGCGQTVSQPYMVAWMTELLRVSEEDRVLEIGTGSGYQTAVLARLA
                     KEVCTVERLPALTVRAQAVLGELNLVNVRYRVGDGTVGWAEQAPYNAICVTAGAPRVP
                     PPLQAQLADGGRLVIPVGDRMLQSLVLVERCGSRYNMRELGGCRFVPLVGAEGWPSGQ
                     T"
     gene            11194..11886
                     /locus_tag="PKK36_03840"
     CDS             11194..11886
                     /locus_tag="PKK36_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNR93723.1"
                     /translation="MKKLICTSAVLLCIMGAGPAAAAPAEHHSIGEKIAGWMRGTGLP
                     DELTVVAISTLPIVELRGAVPVGHMMFPDTDRSTRTGRDDWGRSIRIFILAVIGNMIP
                     IPFILLLLGPVSRWLMRFRIGEKFFNWLFARTRRKAANIEKYETLGLTIFVAIPLPVT
                     GGWTGAMAAFLMGMKFHHAMLSILLGVMIAGVIMTVLSLMGWVGALIAAVVLLSMAAG
                     AVVGLFRREERN"
     gene            11953..12747
                     /locus_tag="PKK36_03845"
     CDS             11953..12747
                     /locus_tag="PKK36_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93724.1"
                     /translation="MTEYTKHIYMTLFPNNALIASHLEPEQFAKHLTVGTAKHYQSKV
                     VFAEVNINFRDPFFEIDEILAQTVPHPVTGKPKRTKFIASYATLEHVALSAIGRLYLV
                     TAGGQVLGIEAQPYTRVSETGRVRVYQEINPLHSLVAANSDPREFGKWITRGTRSKSA
                     PKVCFTQIDMDVDRFAKEHTSRMQFTAPIPEIHPLRLYDCLKELLDNPNKKTKTISLG
                     SVFRHTSYKRIKHGFWIADGDDMLYFPIPPRDELEKNHYDWWREVM"
     gene            12761..13702
                     /locus_tag="PKK36_03850"
     CDS             12761..13702
                     /locus_tag="PKK36_03850"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNR93725.1"
                     /translation="MAALAAEQTVFRVKPTSPFSRVGVLMGGTSAERGVSLRSGASVA
                     KGLTDAGYQVESLIIDSAELVVPDGIEAVFIALHGAFGEDGQIQRMLEEQKIPYTGSG
                     PEASRRAFDKMLSKEIFTRAGLPTPQYAIVKRGEACPLPFPVVTKPVREGSSIGIHIV
                     SDGDDWKASVEDTLRYGEAALVERYIPGRELTVGLVCDQVLPVIEIAPAEGYYNYDAK
                     YQRKDTRYIVPADIPADAAARCRDLAAATFHALGCCGLSRVDFRMTSDSDLYILENNS
                     IPGFTESSLLPKAARAAGMEFPELCDRIMRSAGLNKI"
     gene            13722..14576
                     /locus_tag="PKK36_03855"
     CDS             13722..14576
                     /locus_tag="PKK36_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNR93726.1"
                     /translation="MWWYDDKILTGPRGGRRRRSRESVLYARSGAVRDGWRGHLAGIA
                     LVSVAVIALCGALFAAAMYAGRKLFTENPRFNIAVLDLQSDGTRLSPDHLREYSGLSA
                     GQNLFAVDISEVRRKLELVPLVGRVTVRRSFPDTLEVRVSERSALACLKSDRSAAPLA
                     VDREGYVLGPMYSSPNLPVVEGFSEKGLRPGSQVRAREIRDALSVLDIVDSTPALARH
                     LQIMVVNVRDRDRLDVRLANGKRLLLAPTRLEWRLQRAASSLQADIQKGGNHMLFDVT
                     GDSNVTGQ"
     gene            14607..15806
                     /gene="ftsA"
                     /locus_tag="PKK36_03860"
     CDS             14607..15806
                     /gene="ftsA"
                     /locus_tag="PKK36_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR93727.1"
                     /translation="MPSSPIAAIEVGTSKVCVLVGDLLEDDQLMIVGQGVSPSRGVRK
                     GQVTDLDNVTSCLNHALEQAEEQADVTVSEAYVVISGDHIQSMTNRGSVPVRSPGGLV
                     AEEEVEDVGDLARAVALPTERAILHSIAQRYYLDDQHAVLRPEGMEAARLTLDMLIIH
                     CLRNRIKNVVRTLKEVNVEVADIAFGGLMSGMAVLSPEQKQGGAIVIDLGGGTTDYLV
                     YANMAIAAAGSLSVGGDHVTNDIALGLGLPHGQAEELKKNSGSAVVSPAAGSQTVALQ
                     PEGGFPGKNVRLSDIQTIINARMEETFELVRDSILRSGLRTEMVSGVILTGGGAHLEG
                     AVQLASEIFNRECQIGRPRNVNGLAVVTEGPQYAAPLGMLQHAVRSRNRGRKSGILGA
                     LINLIKG"
     gene            15848..16993
                     /locus_tag="PKK36_03865"
     CDS             15848..16993
                     /locus_tag="PKK36_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR93728.1"
                     /translation="MRETRVVIAGIGGSGCNVMASLARRWPDGPELIGIHTDAAVLSS
                     SHGIRPLQIGAELLNGMSTGGKPDMGRRAAERDRDEISRLFRGSDLVILVTGLGGGTG
                     TGAAPVVAEAAEAAGSLVLCFVMLPFDFEGPHRQEQALQGLGALKGATDAVIQFPNQR
                     VLAQADESLNVTDAFLMADRLVGDCVRSLWELFNRTSLIRLDFADFRSLLMHSEGACA
                     MACVESEGPDRVKHAVAGLLAAPQFEAGAQLRRANALLVGVTGGPDLKLVELQNLLDE
                     IREITHEQARILMGTAVDPDYQGRISLSVLLAEERPEPGPVETPPAETRRDTDRGREH
                     KRVIPLQTTLDLDARGIGRFEDVSPTVHQGANLDVPTYVRRGIRLGR"
     gene            complement(16994..17641)
                     /locus_tag="PKK36_03870"
     CDS             complement(16994..17641)
                     /locus_tag="PKK36_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR93729.1"
                     /translation="MKNEVLSLGPYEANCYLIWDANNDALVIDPGYDGDQVSRTIENY
                     RLNVRAWLLTHGHADHVSGLAAAHAKHPAPVYIHEGDASWVFSPDNHILPYYAAPKKH
                     DGELTIISETQQMEIGSFQLQAIHTPGHSPGGVCYYFPKESLLFSGDTLFKGSAGRTD
                     LRGGDSRALAHSLKLITELPDYTAVYPGHGDRTSLRLEKRSNYFLRQFIAQDGRP"
     gene            complement(17638..19683)
                     /gene="ligA"
                     /locus_tag="PKK36_03875"
     CDS             complement(17638..19683)
                     /gene="ligA"
                     /locus_tag="PKK36_03875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNR93730.1"
                     /translation="MKRDITEAARRAAELRAEIERHNRLYYAEARPEISDYDFDMLLK
                     ELEALEKDFPALMAPDSPTQRVGGAPLSGFESITYSKPMMSLSNVYSKDELRAFDERV
                     RKILGKTAFTYVLEPKIDGVAVSLRYEKGIFTVGGTRGDGRTGDNITANLKTIRSIPL
                     KLHHAGNMPELLEVRGEVYMDRKGFQELNREREEAGLEPFANPRNAAAGSLKQLDSRV
                     AARRPLNAVLYAAGEVRGLVTRSQAELLAQLREFGFPTAPRTWNCSDIEAVLAGLDEL
                     EGMRHDFPFEMDGGVIKINEFGLYEELGATAKSPRWAIAYKYEPEQAETVLLGITVQV
                     GRTGVLTPVAELEPVLVAGSTVSRATLHNEEEIARKDIRIGDRVLIEKAGEVIPAVVK
                     VNLDARTGREKKFSMPAQCPVCGEPTVKREAEVARRCINMQCPAQLKNWIRHFGSRDA
                     MDIEGLGVTLTEQLVDRGMVKTPADLYRLKQEDIAELERMGPRSAASLVRAIQESRGQ
                     NLWRLIHALGIPHVGAGSARTLAGELHTLDRLMSATANELEAVRDVGAVVAQSITSFF
                     SRRDTRELIARLKDAGVNMESGEDAQAAGTALAGKTFVLTGTLKNQTRNQAVERIRRA
                     GGAVSGSVSKNTDYVVAGESPGSKYEAALKHKVKILDEEAFTVLMEAAEGVQKEPDL"
     gene            complement(19683..20066)
                     /locus_tag="PKK36_03880"
     CDS             complement(19683..20066)
                     /locus_tag="PKK36_03880"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNR93731.1"
                     /translation="MGRLFSLWLPPAAWAGLIFGLSSWSGPERPPPLWFPGLDKLAHA
                     VLFGVLGLLLFRAFASSRGMHRAVILAAAVTALYGATDEWHQSFNAARSVELMDFVAD
                     CAGGIAGALAGCALAARWHQRGRLS"
     gene            complement(20113..20637)
                     /locus_tag="PKK36_03885"
     CDS             complement(20113..20637)
                     /locus_tag="PKK36_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93732.1"
                     /translation="MQTRFMLMLLVVLAFGCAKKEPAAAPLPEQPPAEVPFTATNEVV
                     MREEIQIADERKAVEADFDMDGLDDLAVIDPREGHQNEVNIFIQKPADEPSAAPAAAA
                     AASGARLVSPEKGKMVYYRAGVIRQPADGTVIGLMIRKRKDITDLLVLLQDETLNNQM
                     LHYFNDGKKFYLDE"
     gene            20766..>21077
                     /locus_tag="PKK36_03890"
     CDS             20766..>21077
                     /locus_tag="PKK36_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93733.1"
                     /translation="MSQTRGLAMKVLGRDLNFSFGGGKKKNKRVSPIRRPAAVVAQLR
                     ASPFFINVDEGPLTKAVECMEQVRVSDGDVLIREGAEGDYYYLLEEGAATDDQAFDQV
                     AW"
BASE COUNT         4321 a         5888 c         6471 g         4397 t
ORIGIN      
        1 cctgaagacg ccatcaagct tgcggttgaa atgggctgtg acacgatgtt tgtgctgacg
       61 gatgacccgc cctatatcaa gcggggcgat gccgagaccg gcgttgaaat tcccgagcat
      121 caggacaata ttctggattt cgcaaagaac atagagtcga agtacgggcg gcaggtgaaa
      181 atcaacacga tagcctataa accctggtcc aacgcgaaag gccagcgagc cattgatttt
      241 ctgaagaacc tgtcccgttc caccggcgga cgtttcaagc acatcaagcg caaagatgaa
      301 tagcgaagtc ccggaatcac agatccagga ggacaaaccg tctggccggg ccggctttga
      361 ctaccggatg actcatcacg agatcaatga tcttgatctg tccgcctatg acggaccggt
      421 gatgctggtc aactcgccgg agagactgcc ggacgcggtg gccgccttga gcgccgatcg
      481 cattctgggc tttgacaccg aaacgcgccc ttctttcacc aagggcacca actacaagcc
      541 ggcgcttctc cagctggccg gcgagcaggt cgtctgcatt ttccagttga aactcatcgg
      601 gctcccgcgg cccctgatcg atattcttgc gaatcctgag gtcataaagg ccggcgtggc
      661 gccgcagcga gatgctcttg aactcaaggc gctggctgaa tttgagccgg ccggaatgtt
      721 tgatgtgtcc gaggccggcc ggatgggcgg tattcaaaac ttggggctca agggactggc
      781 cgcggtgctt ctcggcgagc gcatttcaaa acgcgcccag ttgaccaact gggcgcggca
      841 gaaactttcc ccgcatcaga ttacatatgc cgcaacagat gcgtggatca gccggcggat
      901 ctatctgcga ctgcaggaga tggacatgct ccgccagccg gtcaggaaaa tggagaagga
      961 atcatgaata agaggattat catcgcattg ctgttgattg cgctggtggc cattgtattg
     1021 atcctcagca gcggcaccac gaccgttaat ttggggtttg gcaggatgca catgcacacg
     1081 ccgctggctc tgctgctgtt cacaagcatt ggggtggttg ttggcctgct cctgaaatga
     1141 ggcgtgcttt acgccttgta aggccccggg cttgtgccat aatctgccaa aaggatcatg
     1201 aattatgatg acggttcccc cccatgttag aaaactgatg gagcaggggg ttttgattcc
     1261 cgctcccgct tcggttgaga ttgacgagcg catcaatccc ttgctgattg cgccgggagt
     1321 tgtgctgcac acagggacga agcttctggg agagcatacc agtatcgggc ccggctgtgt
     1381 gctgggcgag gaaggccccg ttatgctcaa gaactgccag ctgagcagcg gagtgaagct
     1441 gcagggcggc tcatttgagg gttcggtttt tctcggcaga tcttcaatgg gtccctgcgc
     1501 gcatgtgcgg gccgggacgc tgctggagga ggaagccggc ggggcgcatt ccgtgggttt
     1561 caaacagacc atttttcttc cctatgtgac ggccgggagt ctgatcaatt tctgcgacgc
     1621 catgatggcg ggcggtcgca gccgcaagga gcacagcgaa attggatcat ccttcattca
     1681 tttcaactac actccccacc gggacaaggc cacaccgtca ctgatcggag atgttccccg
     1741 cggcgtgatg ctgaacaatg atccgatctt tctcggcgga cagggtggtc tggtggggcc
     1801 ggtgcggatc gattacggtt gtgttacagc cgcggggacg gtttatcgcc gcgatgcgct
     1861 tgagcccaat atgcttctaa cagcgaaatc ccgcggggtc ggccatcccg tgccatacaa
     1921 gtccggagcc taccgcggaa ttgcgcgcat cgtcgcaaac aaccttattt atacaggcaa
     1981 tttgtgggcg ctgacgcagt ggtacaggtt tgtgcgccgc cattttatgg agcgcaatcc
     2041 ctttgatcac gcctgctaca acggggccat tcgactgctg gaagatgcgc gcagggagcg
     2101 cattaaacga ttggccgagc tggcaaagaa ccttaaggaa tccatagcca ttctgtctgc
     2161 aaagggggac ggacccgagc tggccgccca gcacgccttt gtgaaggctt ggcccggcat
     2221 tgaagaagcc gttcgcgggg gcccggagcc ggatttctcc gtggatcagc tgcaggacgt
     2281 tttttccgcg gcctgggacc gcgtctctgg aagcgagaag gactatctca aggcgattaa
     2341 gaacctggcg ccggacgaag cccgtgcggg aaccgcctgg ctgcagtcgg tcgtgaatca
     2401 gattgcctgt ttgttctcaa acgctgaatc atgatccgat gaactacaag gtcaccatag
     2461 gtctggaaac gcatgtgcag ctcaagacgc agagcaagat attctgcggg tgccggaagg
     2521 atttcggcga tgagccgaat acgcatgtct gtccggtgtg cctcggctat ccgggcgcca
     2581 tgccggtgct gaacgaggag gccgtccgcc tcaccgttat gacgggattg atgatcggct
     2641 gtaaaatcaa ttcctacagc aagttcgacc gcaagagcta tttttatccc gacatgccca
     2701 agaactacca gatttcgcag tacgatcagc cgctgtgcct cggcggatcg gttgagatcg
     2761 agacggaggc cgggaagcgt tcgattgagg tcacgcgcat tcaccttgag gaggatgtcg
     2821 ccaagaacat ccatttcgag aatttcagcg gtgtcgattt caaccgggca ggcacaccgc
     2881 tgatggaaat agtgacggag ccggtgatcg aatcggccga cgaggcgttt gcctatctgc
     2941 aggcgctcaa gcagattctg gtttacggcg gcatcagcga ctgcaatctc gaggagggca
     3001 acatgcgctg cgacgtgaac tgcagtgtgc ggcccgaggg gcaggagaag ctgggcgtga
     3061 agaccgagct gaagaacatg aacaccttca agggcgttca tcgcgcgctg aaggctgaaa
     3121 ttgcccgcca gatttccgtg ctcgaaaaag gcggaaccat tcagcaggaa acgcggcgat
     3181 gggatgacgt cgccggcgtc acgctggcga tgcgctcgaa ggaatacgcg cacgactacc
     3241 gctatttccc cgagcccgac ctgatgcccg tggtgctgga cgagaaaact gttgaggaat
     3301 ggcgctgccg gcttcccgaa ctgcccgcga agcggcgcga gcggatgacg gctgagtacg
     3361 gcattcccga atatgatgcg ggcgtgctgg ccgccgacaa gcatgtggcc gactatttcg
     3421 aggagaccgc aaaactttgc ggcagcggga aggcggcctc caactggatc atgaccgagg
     3481 tcatgcgtgt gctttccgcg caggaaattg aaatccgcgg cctcaaaatc acccctgtgg
     3541 cgctggcggc gctgatcaag ctggtggatg ccaagaccat cagcagcacc ggtgcgaaag
     3601 aggtttttgc ggtgatgctc gaggagggcg gcgatccggc ggcgattgtg aaggcgcgcg
     3661 ggctggcgca ggtgagtgac accggcgcgc tggaggcgat ggtgatgcag gcgattgccg
     3721 agaacgagaa atccgtgacg gcctacaggg cgggcaagga agccgctctg cagcatcttg
     3781 tcgggcaggt catgaagctc agccggggca aggccaatcc gggcgttgtc attgagctgc
     3841 tgaagaagaa tcttgcatga acaagattcc ggcaaaagac gggcagaaag agcccgatcc
     3901 attgcttgcg gcattcgtgg attatctgga aggcgaaaag aatgcctcgc accacacggt
     3961 caccaactat cttctcgata ttaaacagtt catccgcttc tcgtggggca atgatgcgaa
     4021 accgccctat ggctggaagc agtgtgaccg ctatgcgggc cgcaagtttc ttgtgcactt
     4081 gatgaaggag gggcgcaatc cgaccaccgt gctgcgcaag ctttccagca tgcggtcatt
     4141 cttccgtttt atgcttcgtg aggaaatggt gaccttcaat cccttctccg gtgtggccat
     4201 gccgaaaaaa gggaaatatc tgccggaggt actttcggtg gcggaggtca accgcctgct
     4261 ggatgctcca aagctggtgg ctgcgaagca gggggcaccc cagtcacctg gcgacaaggc
     4321 ctggatggat tacgcggcgg cccgcgacag ggccattctc gaagtgcttt acagcacggg
     4381 gatgcgtgtc agcgagctgg ccggcctgac gccggggcag gtggattttc tcgcgggcat
     4441 catcaaggtg cgcggaaagg gaaagaagga gcggctatgt ccgctgggcg gcccggcctc
     4501 gcgctcgctg cgcgaggcgg tggacaaaag ggacatgttc tgcgtgagca ttggagaaaa
     4561 acccggctcg gccggatttt tcctgaaccg taagggcggg cggatcacca cgcggtccat
     4621 cgagcggatg atgaagaaat atctgcaggc ggccaatctg aatccggagc attcgccgca
     4681 tgtgctgcgc cacagtttcg cgacgcattt gctcgacaac ggggccgatt tgcgtagtgt
     4741 tcaggagctt ttagggcatg ccagcctttc gaccacgcag atatacacgc acataacggt
     4801 tgagcggctc aaagaggtgt acaaacaggc gcacccgcgg gcgtgaccgg atggattttt
     4861 catgatttgg attgtttata acattctgtt tgctgtcggc ttcacattaa tgctgccgca
     4921 tttcatatat cgcatggtgc ggcgcggcgg ctatgcccga aattttgtcc agcggctggg
     4981 cttctacagc cggtctatcc gcacaaaact ttccgccgga cgccacatct ggattcaggc
     5041 cgtgagcgtg ggagagatat ttgttgcctt ccgcttcatg gaggaaatcc ggcgcaggaa
     5101 tccggatgca cgctttgttc tgtccaccaa tacctcgacg gggtacgccc ttgcccggag
     5161 caggattggc gaaagtgata ttctcattta ttttcccgtg gattttccgc ttgtgatgcg
     5221 gcgcgtgctc gggctgatca atcccgcggc tcttattctt gttgaatgtg aactctggcc
     5281 gaatctcatc cgtctggcaa agagaagggg caccccggtc ctgctggtca atgggcgcat
     5341 ttcggagcat tcgttcaggg gctatcgcaa attgaagttc tttacaaaga agattctccc
     5401 gatggtggat ctgctctgcg cccagagtgc cgccgatgcc cgccggctga tcagccttgg
     5461 cgccccagaa cggaatgtga aggtgatggg cagcgccaag tatgaggtcg catcgaaaga
     5521 tcccacgggg gaagaaaagg cggcagccat tctgaaagcg gccgggattg acatcacggc
     5581 gccgattctc ctcggcggtt ccacctgggc gggagaggag aaaatcctgc tcaacgcatt
     5641 caagattctg cgggatgaat tcaagaacct gcagcttgtt cttgccccgc gccatgcgga
     5701 gcgggcaggg gaggttattc acgaaatcga atccgcagga tgccgcgcag tgcagcgctc
     5761 aaaactggat ctgtcggccg cgcgtgtctg ggagccggac gatgtgcttc tggtggacag
     5821 cactggcgag ctgaagaatt tctatcctta tgccaccgtg atcttcatcg gcaagagcct
     5881 gacgcagcac ggcgggcaga atcccatcga gcctgcggtg tacggaaagc ccgtcatcgt
     5941 ggggccgaac atggaaaatt tcccggtggt catggaggat ttcagggaag cgtcggcgct
     6001 gatccaggtc gcggatgaaa cggggctgca ctcaagcata cgtgccctgc tgtccgacgc
     6061 acagctccga ggggattacg gaggcagggc ggcatctctg gtcgaggcca agtccggggc
     6121 cctggcggaa actgttgaac ttataattcc gcaccttttg aaatcatgaa tttcaacttg
     6181 ccgcgactgg ctttatcggc gagtatcgca ggcaatggaa tacaggaggg tttgatgaaa
     6241 gcattgattc cgctcatgct gtgtatgctg actgtcggtg cggggtttgc tgacaccacg
     6301 gttgatgcct ctaatcccta tgcctatggg gcaaacatcg gctggatcaa tgcccggggg
     6361 gatatcgcct ccggcgcagt gttcggacag tcttattgca ccggctatct gtacagcgcc
     6421 aactgcgggt ggatttcgct gggcaacgga cctaccaacg gctggcgcta ttccaatacc
     6481 gccgccgacg actggggtgt caaccacgac ggaacaggaa gcctgacggg ttttgcctat
     6541 ggcgcaaaca tcggctggat tgcttttgaa cagacctatg gacagccccg ggtggatctg
     6601 ctgaccggaa atctttctgg atacgtttgg ggtgccaatg tgggctggat cagtctttcg
     6661 aatgcgcagg cttatgtgca aaccaccagg cttgactctg gacccgactc cgatgccgac
     6721 ggcattcctg atttctggga gatgaagcag gtcggggacc tggctacgct cagcggggcg
     6781 gggcacgacg aggatgatga tggcgcttcg gacactgatg aatattcttc agatacagat
     6841 ccgacggata ttaattccag tcttgagatc acggcgctcg aacgtctgga tggcacaaat
     6901 cttgtgacat ggagcattga gcccactcgt ttctacaagc tggaacatgc ctccagtgcg
     6961 agtaacaatg cggattgggg ggacagcggt cacggaatca tgtctccgga actggcggat
     7021 gaaatgacgc gagaactggc cgattccgca caaacgtcgc agttcttccg ggccagagcc
     7081 attgtccctc tgtcgccctg aaaagccgga aataaggcca aaaatgccct aaaaatggca
     7141 aaaaacgcct aaaaacggcc gaaaatgccc caaaaacggt aaaaacgacc tcaaaacggc
     7201 tagttttatc agaaaaacag catttcgctt ttccggcgca atgtgttcca cattgaaatt
     7261 ggagagggaa attcatatgc tggcaggcat tcgaattgaa tccagcgagg gaaaacatgc
     7321 acaagagaat atcggcggca cggagcccgg aatggaaggc gctgcaggct catcaaaaag
     7381 aaataaaatc gcagcacctg agggatcttt ttcagcagca tcctgaacgg gcggaaacgt
     7441 tctcgctcga ggcctgtgac ctgtttcttg actattccaa gaatcgcatc actttcgaga
     7501 caatgccgct tctgctgaag ctgacagaag tggcccgtct gcgcgatgaa atccgcgcaa
     7561 tgttcagcgg cgagaaaatc aacgcaactg aaaaccgcgc cgtgcttcat gccgcgctgc
     7621 gcaatgtgtc gaatcgcccg attatggtgg acgggaagaa tgtcatgccc gatgtgcgcc
     7681 gggtactgga tcagatgagg gatttctcca cgcggatacg cagcggagag tggaaggggt
     7741 ttaccggaaa gaaaatccgc cacgtggtga atatcggcat tggcggttct gatctgggcc
     7801 cggtgatggc ttccgaagcg ctgaaggcgt acagcgaccg tgatatcacc ctccattttg
     7861 tttccaatat tgacggcacg catctggcgg aaacgctgcg caagctgaac gccgatgaaa
     7921 cgctgttcat cgtggcctcc aagacgttca ccacgcagga gaccatgacc aacgcgcaca
     7981 gcgcgcgggg gtggtgcctg gcttcgctga aggatccggc cgccatcgca aagcattttg
     8041 tcgcactgtc caccaacacg gaagcggtaa agagctttgg tatagatcct gccaatatgt
     8101 ttgagttctg gaactgggtc ggcggccgct attccctctg ctcggcgatc gggctcccgc
     8161 tgatgatttc catcggtccg gaccacttta tggaaatgct gcgaggattt catgcgatgg
     8221 actgtcattt cgaaagcgcg cctttcgaac agaacatgcc cgtaatcctt gggctgctgg
     8281 gcatctggta caacaatttc tttgacgcgg aaacgcatgc catactccct tatgatcagt
     8341 atctgcaccg ctttgcggcc tatttccagc agggcgacat ggagagcaac gggaaatatg
     8401 tggaccgaaa cggcagcccg gtgaaatacc agaccgggcc ggtgatctgg ggcgaaccgg
     8461 gcaccaacgg ccagcatgcg ttctaccagc tgattcatca gggaaccaaa cttatcccgg
     8521 ccgatttcat cggctttgtg cagtctctga atccgatcgg ggaccatcac gacaagctga
     8581 tggcgaattt ctttgcccag accgaggccc tggcttttgg caagaccgcc gacgaagttc
     8641 gggctgaagg cacccggcgg gcgctggttc cgcaccgcgt ttttcaggga aaccggccga
     8701 caaacacgat tatggcctcc aaactcactc cggcggtgct gggacagctc attgcgctct
     8761 acgagcacaa gattttcaca cagggaatca tctggaacat atattccttt gaccagtggg
     8821 gtgtggaact gggcaaggtc ctggcgacga agattttgaa agaactgcag gatcccgctt
     8881 ccgcccccgt gcatgattcc tcaacgagca ggctgatcag tcgctatcga gccgaacgga
     8941 aggcgtgaaa tgcagcagtg ccctcccaaa agacttccgg cctggatgcg aacctcgcgt
     9001 cggactgatc gcagcttcgt tcgcgttcag cggctgctgg aagcggagtc tctgtgcacg
     9061 gtctgccagg atgcgaaatg tccaaaccgt cttcaatgct ggaacgaggg cacggccacg
     9121 ttcatgatcc tggggcatgc ctgcacgcgc aactgccgtt tctgcgcggt gcaaaagact
     9181 gttccccggg aaccagatcc aaacgaaccc gcgagcgtgg cgcgggccgt gaaggccatg
     9241 aatctcaggc acgcggtgat caccagtgtg acaagggacg atctgccgga tggcggcgcc
     9301 gggcattttg ccgggaccgt tcgcatgata cgcgcggccg gcgtgaccgt cgaagtgctg
     9361 gtcccggact tcaacggctg cgcggcggcg cttgaaaccg tgctggatgc ccgcccggat
     9421 gtgttcggcc acaatattga aacggtacgg cggctgcagg ccgtggtgcg gcccatggcc
     9481 tcctatgaaa gatcgcttgc ggttttgcgc gcggcagcgg ctcatggcgg tgcgctggtc
     9541 aagtcgggcc tgatgctggg aatgggggaa acaagagcgg aaatccttca ggcgatggac
     9601 gacctgctgg aggcgggatg cgagcttctg accatgggcc agtatcttgc cccgacgcct
     9661 gaccactatc ctgcggcttc ttatctggcg ccggaggagt ttgatgaact gggagacgcg
     9721 gccagagcga aggggttcag ggcggtggca tcgggtccgc tggtgcggtc atcctacagg
     9781 gccgcgcaga tgttcgtgca ggcgcagggg gcaaagccat gaatccattg cctttcaaac
     9841 ccagatactg tgtgctgatc ccggcctatc aggaggaaaa aaacattgcc tctgtggtgc
     9901 gggatgccct gaaatactgc ccgcaggtga ttgtcgtgga tgacggatcc ggcgacaaga
     9961 ccgcttcggt ggcggatcag gccggggcca tcgttcaccg gcatgagacc aatcggggga
    10021 agggggccgc cctgcagacg ggttttgccg tggcccgcgc ccgcgaatgt gatgccgtga
    10081 tcaccatgga tgctgacggc cagcacaagc cggaggaaat cccccgcttc atggatgcct
    10141 atgagcgcac ccgtattccc gtgctggtgg gcaaccggat ggctgatccc gccggcatgc
    10201 cgctcatcag aaggctgacg aaccgtttca tgagctgcat gctcagccgg gtaatgaagc
    10261 agtacgttcc ggacacgcaa tgcggtttcc ggctgtttgc aagcgatctc actccttttg
    10321 tccccacgga gtcggccggt ttcgccgcgg aatcggaaat ccttctttat gtggcgcgcc
    10381 gcggcatccg aatggattcc gttcgcgtgt ccaccgtcta taccggcgca gagcagagca
    10441 gtattcgccc gttcagtgat gcgttccggt ttttcggcat gctgtggcgg aaccgggaac
    10501 ggaagaggtt ctgatgcagg aaagtgccgc cgcccatctg cgccgggagc atattgaggt
    10561 tttcatccgc ggccggggtg tctgcgataa acgggtcctc cgggccctgc aggatgttcc
    10621 cagggagctc tttgctccgg gaatgtctct gaacgatgtc tacggcgacc atccccttcc
    10681 gatcggctgc ggccagacgg tttcgcagcc atatatggtt gcctggatga cggagcttct
    10741 gcgcgtttcg gaagaagatc gcgtgcttga gatcggaacc ggatccgggt atcagacggc
    10801 tgtgctggcc cgtcttgcga aagaagtatg cacggtcgaa cgccttccgg cgctcacggt
    10861 gcgcgcgcag gccgtacttg gagaactcaa tctggtcaat gtcaggtacc gggtggggga
    10921 tggaactgtc ggatgggcgg agcaggcgcc gtataacgcc atttgcgtca ccgccggggc
    10981 gccccgtgtt cctccgccgc ttcaggcgca gctggcagac gggggccgcc ttgtcattcc
    11041 ggtgggagac aggatgctcc agtcgcttgt gcttgtcgaa cgatgcggca gccgctacaa
    11101 catgcgcgaa ctgggcggat gccgcttcgt acccctggtg ggggcagagg gctggccgtc
    11161 cggccaaacc tgatattgcg tggagatatt aaaatgaaga aactgatatg cacatcggct
    11221 gttttgcttt gcatcatggg cgcggggccg gcggccgcag cgcctgcgga gcatcattcc
    11281 atcggggaga agattgccgg ctggatgcgc ggtacaggcc tcccggatga gctgaccgtc
    11341 gtggcaatat ccacgcttcc gatcgtggag cttcgcggag ctgttccggt gggccatatg
    11401 atgttccccg atactgaccg ctcgacccgc acgggccgcg acgactgggg ccggtccatc
    11461 cgtattttca ttctcgccgt gatcgggaac atgatcccca ttcccttcat tctcctgctc
    11521 ctcggacctg tttcgcggtg gctgatgcgt ttccgcatcg gggaaaaatt cttcaactgg
    11581 ctgttcgccc ggacccgccg caaggcggcg aacatcgaga agtacgaaac cctggggctc
    11641 accatatttg tggccatccc gctgcctgta acaggcggat ggaccggcgc catggcggcg
    11701 tttctcatgg gcatgaagtt ccatcatgcc atgctgtcca tccttctggg ggtgatgatt
    11761 gccggtgtga tcatgacggt cctgtcgctc atggggtggg tgggagccct gatcgccgcc
    11821 gtcgttttgc tgtccatggc ggccggagcg gtggtcgggc tgtttcgccg agaagaaagg
    11881 aattgaatcc ggcctgcaaa tgatataagc atgagactgg aatgatctgc tgcgcctaaa
    11941 caggagaaga cgatgacgga atatacgaaa catatataca tgacgctgtt tccaaacaac
    12001 gcgctgattg catcgcatct tgagccggaa cagtttgcaa aacacttgac cgttggcacg
    12061 gccaagcact atcagagcaa ggtggtcttt gccgaggtga atatcaactt ccgggatccg
    12121 ttttttgaga tcgacgaaat ccttgcgcag acggtgcccc atccagtgac cggcaaaccc
    12181 aagcggacca agttcatcgc ctcctatgcg acactggagc atgtggcgct ttcggccatc
    12241 ggcaggctgt atctggtgac ggccggaggg caggtgctcg gaattgaagc tcagccgtac
    12301 acgcgcgtca gcgagaccgg ccgcgtgaga gtttaccagg agattaatcc actgcacagt
    12361 ctggtggccg ccaacagcga tccgcgcgag ttcggaaaat ggatcactcg cggcacccgc
    12421 tcaaagagcg cacccaaggt ctgttttacc cagatcgata tggatgtgga tcgcttcgca
    12481 aaagagcata ccagccggat gcagttcact gccccgattc ctgaaattca tccgcttcgg
    12541 ctctatgact gcctgaaaga gctgctggac aatccgaaca agaagaccaa gacgatcagt
    12601 ctcggctctg tgtttcgcca tacttcctat aagcgcatta agcatggctt ctggattgcc
    12661 gatggcgacg acatgctcta cttcccgatt cctccccgtg atgaactgga aaaaaaccat
    12721 tacgactggt ggcgtgaagt gatgtgaggc agccggagcc atggcggcat tggccgcgga
    12781 gcagacggtt tttcgtgtga aacctacttc accattcagc cgggttggtg ttttgatggg
    12841 gggaacttca gccgagcgcg gggtttcgct ccggtcgggt gcttccgtcg caaaaggcct
    12901 gacagacgcg ggatatcagg tcgaatccct tatcatcgat tcggctgagc tagtggttcc
    12961 tgatgggata gaggcggttt ttattgctct gcacggcgca ttcggtgagg acggtcagat
    13021 ccagcggatg ctggaggaac agaaaatccc ctataccgga tcaggaccgg aagcaagccg
    13081 ccgcgcgttt gataaaatgc tgagcaagga gattttcact cgcgcagggc ttccgactcc
    13141 gcagtatgcg atcgttaaaa ggggcgaagc ctgcccgctc ccgtttccgg ttgtcaccaa
    13201 gccggtccgc gagggatcca gcattggcat tcatattgtg tccgacgggg acgattggaa
    13261 agcttccgtg gaagatacgc tccgatacgg tgaggcggcg ctggtggagc gctacattcc
    13321 cggccgcgag ctgacagtcg gcctggtttg tgatcaggtg ctgccggtca tcgagatcgc
    13381 ccccgcggaa ggctattaca actatgacgc aaaatatcag agaaaagaca cccgctacat
    13441 tgtcccggct gacattcccg ctgatgccgc tgcccgctgc agggatctcg ccgcagcgac
    13501 ttttcatgca ctcggatgct gcggtctcag tcgcgtggat tttcggatga cttccgacag
    13561 cgatctctat atattggaaa acaacagcat tcccggtttt acggaatcca gtcttttgcc
    13621 caaggcggcc cgggcggcgg ggatggaatt ccctgagctg tgcgatcgca tcatgcggtc
    13681 cgcaggactg aacaaaatct gatcgtgagg atggtcttgg catgtggtgg tatgatgaca
    13741 aaatcttgac cggccccagg ggggggcggc gcagacgaag ccgtgagtcc gtgctgtatg
    13801 cacgctccgg cgctgtccgc gacggctggc gcggtcatct ggccgggatt gccctggtca
    13861 gcgtggcggt gattgctctc tgtggagcgc tgtttgccgc agccatgtat gccggccgga
    13921 agctgttcac ggagaatccc cgattcaaca ttgcggtgct cgatctgcag tcggacggga
    13981 cacgcttgtc cccggaccat cttcgcgaat attcgggcct ttcggccggc caaaatctgt
    14041 ttgcggtgga tatttcagaa gtgcgccgga agcttgaact ggtcccgctg gtgggccgcg
    14101 tgactgtccg ccgcagtttc cctgacaccc tggaagtgcg tgtgagtgag cgcagtgctc
    14161 tggcctgtct gaaaagcgac cgcagcgccg cgccgctggc cgtggaccgc gagggttatg
    14221 tgctgggccc gatgtattct tctccaaacc tgccggttgt ggagggcttt tcggaaaagg
    14281 gcctgagacc cggaagccag gttcgggccc gggaaatccg tgacgccctt tccgttctgg
    14341 acatcgtgga cagcacgcct gcactggccc ggcatctgca gatcatggtg gttaatgtca
    14401 gggaccggga ccggctggat gtgcgcctgg caaacgggaa gcgtctgctg ctggctccca
    14461 cccgtcttga atggcggctt cagcgggccg cctccagtct gcaggcggat attcagaagg
    14521 gtggaaatca tatgctgttt gatgtaacag gagacagcaa tgtcactggc caataagata
    14581 cacaggaccg cggagggcgg ctgattatgc cctcatcacc catagcggcc atcgaagtcg
    14641 gaacatcaaa ggtctgtgtg ctggttgggg accttcttga ggatgatcag ctgatgatcg
    14701 tcgggcaggg cgtcagcccg tcccgcggtg tgcgcaaggg gcaggtcacc gatctcgaca
    14761 acgtgacgtc ctgcctgaat catgcgctgg agcaggccga ggagcaggcc gatgtcaccg
    14821 tcagcgaggc ctatgtggtc atcagcggag atcacattca gtccatgacc aaccggggca
    14881 gtgttccggt gcgttcgccc ggaggcctgg tggccgaaga agaggtggag gatgtcggtg
    14941 atctggcccg ggctgtggcc ctgcctacag aacgggcgat tctccattcc atagcccagc
    15001 gctactatct ggacgaccag cacgcggtgc tccggcccga ggggatggag gcggcccgtc
    15061 tcacgctcga tatgctgatc attcactgcc ttcgcaaccg catcaagaat gttgtgcgca
    15121 cgctcaagga ggtgaatgtg gaggtggcgg atattgcttt tggaggcctt atgtcgggca
    15181 tggcggtgct ttcaccagag caaaaacagg gcggcgcaat cgtgattgat ctcggcggcg
    15241 gcaccaccga ctatctggtt tatgccaaca tggccattgc ggcggctgga agcctttcgg
    15301 tgggcggaga ccacgtaacc aatgatattg cccttgggct gggacttccg cacggtcagg
    15361 cggaggaact caagaagaac tcaggttccg ccgtggtcag cccggccgcc gggtctcaaa
    15421 ccgttgcgct gcagcccgaa ggcggtttcc cggggaaaaa cgtgcgtctg tcggatatcc
    15481 agacgatcat caacgcgcgc atggaagaga cctttgaact ggtcagggac agcattcttc
    15541 gcagcggtct gcgcacagaa atggtatccg gagtcatttt gaccggcggc ggagcccacc
    15601 ttgaaggagc ggttcagctg gccagtgaaa tctttaatcg cgagtgccag attggccgtc
    15661 cgcggaatgt gaacggactt gctgtggtca ccgaaggacc ccagtatgcc gctcctctcg
    15721 gcatgctgca gcatgcggtg cgcagcagaa accgcggaag gaaatccggc attttgggcg
    15781 cgttgatcaa tctaattaaa ggctgagaca caggatgcag ttccacaggg aaaaccgaga
    15841 cagagcgttg cgggaaacgc gggtggtgat cgccggcatt ggcggcagtg gatgcaacgt
    15901 catggcgtcg ctggcccggc ggtggccgga tggccctgag ctcataggca tacacacgga
    15961 tgccgccgtg ctctcctctt cgcacggcat tcgcccgctg cagattggag cagaacttct
    16021 caacgggatg agcaccggag ggaaaccgga tatggggcgg cgcgccgccg aacgcgaccg
    16081 ggatgaaatc agccggcttt tccgcggttc ggatctagtg attcttgtga cgggtctcgg
    16141 cggcgggacc ggcacggggg cggcgcccgt tgttgccgaa gcggccgagg cggccggatc
    16201 tcttgtgctc tgctttgtca tgctcccgtt tgatttcgag gggcctcacc gtcaggaaca
    16261 ggctttgcag ggcctgggcg ccctgaaggg ggccaccgat gccgtgatac agtttcccaa
    16321 tcagcgtgtg ctggcccagg cggatgaatc gctcaatgtc acggatgcat ttctgatggc
    16381 agaccgtctg gtgggcgact gcgtccggtc gctctgggag ctgttcaacc gcaccagcct
    16441 catccggctt gactttgctg atttccggag cctgctgatg cacagcgagg gcgcgtgcgc
    16501 catggcctgt gttgagagcg agggtccgga tcgcgtcaag catgccgtgg ccggtctgct
    16561 ggccgccccg cagtttgagg ccggcgcgca gttgcgccgg gcgaatgccc tgctggtggg
    16621 cgtgaccggc ggcccggact tgaaactggt tgaactgcag aacctgctgg atgaaattcg
    16681 ggaaatcaca catgaacagg cccggatact gatgggtacg gcggtggatc ccgattatca
    16741 gggccggatt tcgctgagtg tgctgctggc ggaggagcgg cctgaaccgg ggcccgtgga
    16801 aaccccgcct gcagaaaccc ggcgggacac tgatcgcggg cgtgagcata aacgcgtaat
    16861 cccgctgcag accacccttg atcttgatgc ccgggggatc ggccgttttg aggacgtttc
    16921 ccctacggtg catcagggag ccaatctgga tgtgcccacg tatgtcagac gcggcatccg
    16981 gctgggccgg tgatcacggc cgtccgtcct gtgcaataaa ctgccgaagg aaataattgc
    17041 tgcgcttctc aagtctcagc gaggtccggt ctccatgacc cggatagacg gctgtataat
    17101 ccgggagttc ggtgatcaat ttcagtgaat gcgccagggc gcgtgaatct ccaccccgca
    17161 gatcggttcg gcccgccgac cccttgaaaa gcgtgtcgcc ggaaaacaga agggattcct
    17221 tcgggaaata atagcagacg cctccaggcg agtggccggg cgtatgaatg gcctgcaact
    17281 ggaaggagcc aatctccatc tgctgcgtct ccgaaatgat ggtcaactcg ccgtcatgct
    17341 ttttcggcgc agcatagtag gggaggatat ggttgtcggg cgagaacacc cacgacgcat
    17401 ccccttcatg aatatacacg ggcgcgggat gctttgcatg ggccgcggca agtccgctga
    17461 catggtctgc atgcccgtgc gtcagaagcc atgcgcgcac gttcagccgg tagttttcaa
    17521 tcgtgcggga cacctggtct ccgtcatatc ccggatcaat gaccagggca tcgttgtttg
    17581 catcccagat caaataacag ttggcctcat agggccccag gctcaatact tcattcttca
    17641 caaatctggc tccttttgaa cgccctccgc ggcctccatg agaactgtaa atgcttcttc
    17701 gtccagtatc ttcaccttgt gtttcagcgc ggcctcgtat ttggaccccg gactctcgcc
    17761 ggccaccaca tagtctgtat tttttgaaac acttccgctc acggcgcccc cggcgcgccg
    17821 aatccgctcg accgcctggt tccgggtctg gtttttcagt gtgcctgtca gaacaaacgt
    17881 tttcccggcg agagcggttc cagcggcctg cgcatcttct cccgattcca tgttgacccc
    17941 ggcatccttc agccgggcaa tgagttcccg ggtgtcgcgg cggctgaaaa aagaagtaat
    18001 gctctgcgcg accaccgccc ccacatcccg cacggcttca agctcattgg ccgttgcgga
    18061 catcagtcgg tccagcgtgt gcagttctcc cgccagagtg cgggccgatc cggcacccac
    18121 atgcggaatg cccagggcat ggatcagccg ccagaggttc tgtccgcggc tttcctgtat
    18181 cgcccggacc aggctggcgg ccgatctggg gcccatgcgt tcaagctccg caatatcctc
    18241 ctgcttcagc cgataaaggt cggcaggcgt tttaaccatc cctcggtcaa cgagctgctc
    18301 ggtcagcgtg acgcccagtc cttcaatatc catggcgtcg cgggatccga aatgccgtat
    18361 ccagtttttc aactgcgcag gacactgcat gtttatgcag cgccgggcca cctctgcttc
    18421 gcgcttgacg gtcggttctc cgcacaccgg gcattgcgca ggcatggaaa atttcttctc
    18481 ccggccggtt ctggcatcca gattgacctt caccacggcg gggatcactt ctccggcctt
    18541 ttcgatgagg acgcggtcgc cgatgcggat atctttccgg gcaatctcct cctcgttgtg
    18601 cagtgtggcc cggctgacgg tggaccccgc cacaagaaca ggctccagtt cggccacggg
    18661 cgtcagcacg cctgtgcgtc ccacctgaac ggtgatgccg agcaggacgg tttcggcctg
    18721 ttcgggttcg tacttgtagg ctatggccca ccgcgggctt tttgcggttg cgccgagttc
    18781 ctcataaagg ccgaattcat tgatcttgat gacgccgcca tccatttcga acgggaaatc
    18841 atgccgcatg ccttccagtt catccagtcc tgcaaggacg gcctcgatat cggaacagtt
    18901 ccatgtgcgc ggtgcggtgg ggaatccgaa ctccctgagt tgagccagca gttcggcctg
    18961 ggagcgtgtg accagccccc ggacttcgcc cgcggcgtaa agcaccgcat tcagggggcg
    19021 cctggccgca acgcgggaat ccagctgctt gagcgaaccc gccgccgcat tccggggatt
    19081 ggcaaagggt tccagcccgg cctcctcccg ttcgcggttc agttcctgaa atccttttct
    19141 gtccatatac acttccccgc gaacctccag cagttcgggc atgttcccgg catggtgcag
    19201 cttgagcggg atgctgcgga tggttttcag gttcgcggtg atattgtcgc cggtccggcc
    19261 gtcgcctcgc gttccaccca ccgtgaatat gcctttctca tagcgaagtg aaacagccac
    19321 gccgtcaatt ttcggctcca gcacataggt gaacgcggtt ttcccgagaa tctttcgcac
    19381 ccgttcgtca aaggcgcgca gctcatcttt gctgtagaca tttgaaagac tcatcatggg
    19441 ttttgaatag gtgatggatt caaaaccgct caggggggcg ccgcccacgc gctgggtggg
    19501 ggagtctgga gccatcaggg ccgggaagtc cttttcaagg gcttccagtt ccttcagaag
    19561 catgtcaaaa tcataatcgc tgatttcggg ccgggcctct gcgtaataca gccggttgtg
    19621 ccgctcaatt tccgcgcgca gttcggccgc gcgccgtgcc gcctcggtga tgtctctttt
    19681 catcatgaca atctgccccg ctgatgccat cgggccgcaa gagcgcagcc ggcgagagcc
    19741 cccgcaattc ctcctgcgca gtctgcgaca aaatccatca gctcaacgga tcgggccgca
    19801 ttgaaggact ggtgccactc atcggtcgcc ccgtagagcg ccgtgacggc cgccgccaga
    19861 attacggccc ggtgcatacc gcgggatgag gcaaacgccc tgaacagcag cagccccagc
    19921 acaccgaaaa gcacagcatg cgcaagcttg tccagccccg gaaaccagag cggcggcggc
    19981 ctctcgggtc cgctccatga gctcaggccg aaaatcagcc cggcccatgc ggcgggcgga
    20041 agccacagcg aaaataatct ccccatatgc gaatatctgc tgtgtgcagg actggatctc
    20101 tgcccggcgg gtttactcat ccagatagaa cttcttcccg tcgttgaaat agtgaagcat
    20161 ctgattgttc agagtctcat cctgcagcag caccagaagg tccgtgatat ccttgcgctt
    20221 ccggatcatc agcccaatca cggtgccgtc cgccggctgc cggatgactc cggcacggta
    20281 gtagaccatc ttgcccttct ccggactgac cagtcgcgcg ccgctggccg cggccgctgc
    20341 ggccggggcg gccgacggct catctgccgg tttctggatg aagatgttca cctcgttctg
    20401 atggccctcc cggggatcaa tcactgcgag gtcgtccaga ccgtccatgt caaaatcagc
    20461 ttcgaccgcc ttgcgttcat cggcaatctg tatttcctcg cgcatcacga cctcgttggt
    20521 ggccgtgaat ggaacttccg ccggcggctg ttcgggcagg ggagccgccg caggttcttt
    20581 cttcgcgcat ccaaaggcaa ggaccactaa cagcatcaac atgaatcttg tctgcatagc
    20641 caactcctaa ttgcagtgtc agtgtgccat gtccgcatta atcaatcaag aatatccgct
    20701 ctaaaacctg aaagataaaa taatcattcg aaaatctggc catttacggc cggaaatggc
    20761 attctttgag ccaaacgaga gggctggcca tgaaagtgct gggacgcgat ttgaacttca
    20821 gtttcggggg cgggaagaag aagaacaaaa gagtatcacc catccgccgg ccggcggctg
    20881 tcgtggcgca gctccgcgct tcacctttct ttatcaatgt cgatgaaggg ccgctcacca
    20941 aagccgttga atgcatggaa caagtacggg tttcagacgg ggatgtgctg attcgcgaag
    21001 gggccgaggg cgactattac tatctgctgg aggagggcgc ggccaccgac gaccaggcct
    21061 tcgaccaggt ggcgtgg
//
LOCUS       DAOQWR010000029        20789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2930_length_20789_cov_5.198418, whole genome shotgun sequence.
ACCESSION   DAOQWR010000029 DAOQWR010000000
VERSION     DAOQWR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20789
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_20789_cov_5.198418"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..3846
                     /gene="nifJ"
                     /locus_tag="PKK36_03895"
     CDS             298..3846
                     /gene="nifJ"
                     /locus_tag="PKK36_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNR93734.1"
                     /translation="MKKKIAIMDGNTAAAYVAHKTNEVCAIYPITPSSNMGELADQWS
                     AEGKTNIWGTVPHVVELQSEGGASGAVHGALQGGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVSARTLATHALSIFGDHSDVMAARATGWAFLASASIQEAMDMALIAQAAT
                     LEARVPIVHFFDGFRSSHELNKIEVLGDETIREMISEDLVLAHRARALTPDRPVLRGT
                     AQNPDVFFQGREAINGFYTAAPAVVQKAMDKFAKLTGRQYHLFDYHGAPDAERVMVIM
                     ASGAETALETVDTLLEKGEKVGVLIVRLYRPFSPQHLLKVIPATTKALAVLDRTKEPG
                     ADGEPLYKDIVTALAQAAADGKLPTKTMPKVVGGRYGLSSKEFTPGMVKAVLDELKKD
                     KPKNSFTVGIHDDVTHSSLEYKTFLTNAPKQVRCMFFGLGSDGTVGANKNSIKIIGEE
                     TDNYAQGYFVYDSKKAGSRTVSHLRFGPTPIHSTYLVQSANFIGCHQFVFLEQTDILG
                     QAADGGVFLLNSPYPKEEVWDKLPAKVQKQIIDKKLKFYVIDGYHVAEQAGMGTRINT
                     IMQTCFFAISGVLPKEEAIEKIKQAIKKTYGKKGEDVVQKNYVAVDAALAHLHEVAVP
                     DHVTSRIDMPPVVAPEAPEFVQKVTAEIMAGRGDDIPVSAFPVDGTYPLTTTKWEKRN
                     VALSVPQWDPDVCIQCGTCGFVCPHACIRSKKSTEAELKSAPEGFKSAPLKGKDAPAD
                     ARFTLQIAVEDCTGCGLCVMACPAKNKQIEGRKAINMADKLPIMDEARAAFKYFDEVL
                     PDTDRSTLNLALLKDIQFARPLFEFSGACAGCGETPYVKLMSQLFGDRMVIANATGCS
                     SIYGGNLPTTPYAVNQDGRGPAWSNSLFEDNAEFGYGFRLAYDKHSELAAELLKKLGG
                     QIGDKLVDELLNSDQSTEGGIAEQRRRVAALKEQLKGIKSDEARNLASIADMLVKKSV
                     WILGGDGWAYDIGYGGLDHVLASGRNVNAMVLDTEVYSNTGGQASKATPRGAVAKFAA
                     GGKPMAKKDLAMMAMCYGNIYVARIALGANPRQAINALLEAEAYDGPSLIVAYSHCIA
                     HGINMINGLEQQKLAVKSAYWPLFHYNPSLAAEGKNPLVLDSKAPAMPLKDYAYNEVR
                     YTMLTKSKPNEAARLIELAQADVDKRWKMYEKWAQE"
     gene            3944..4132
                     /locus_tag="PKK36_03900"
     CDS             3944..4132
                     /locus_tag="PKK36_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNR93735.1"
                     /translation="MKVKIDEDACTGCGLCCDTCPDVFEMGDTVAAVKVAEVPEDAEE
                     TCQEAVDSCPVECITVVE"
     gene            4242..6551
                     /locus_tag="PKK36_03905"
     CDS             4242..6551
                     /locus_tag="PKK36_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93736.1"
                     /translation="MNNGCSRELHEALRDQAAGRTLIFRRGPDCYDPGDVLEYEVTGI
                     QPCTKAQARLKVDKFLGGGFAGQVYLCTLSGLSGGTIPGLVDGRRYAVKIMQPPDGAA
                     TLFRNFIYWLAFQGPFSAQVSEAASRSGLLWQKLFRKAGELIFGRSTSVKDVYASFCD
                     ENLRAYGEITEWVEGRTWRLESDPHIRKRRHWETIDLEKTASAEYIAKRRFMRDMVEL
                     MHDMGASEFARQYEWSTMKSQPNVLKRTDVDESGPGGGLCAIDFRAGLALLPFLPMSP
                     GDFRLILQGLFRRKSLVQFDRCNLRKLDEFIARHAGHFKDDAAMIEELKACDRKYRRS
                     LPDITHHGWRLLFDRELRREVRRGLICGYRMNRLTDEDFAGKLEKSAGLFAVYYFLGA
                     FPVLGRILRQIAGNSLYRQHLKHAAGSWAYMQEALSARCARGLVLWLRKGRVNEGHAL
                     GLAKHPGLFLMERWTLGLLPAGLHRAVSEPLYAFRRLKSFLLFLKNFISNAAYREKWF
                     LDQIEDGRRQGMLTGEEYTEIRNHVQDPFIVKYLKCLGVHFATLPVTQIVSVAVGGIV
                     AFKMLAAGQSRTDASLAFAAVVALFQVLPISPGSICRGLFVLYLMLRERNFRDYMVAM
                     PLSFVKYIGYLAFPLQMTATYPKLARFMAGRWATSAVHIIPVFGEQGALLEHWVYDLF
                     FNVPQAFGIWARPRVRMLLNIWMALGLALGGWLIAHFNWPLNEGPAVNTIIATLCLFA
                     LPRVLFYPLLNRQKGLRSSQAPAHPPNGN"
     gene            complement(6490..8388)
                     /locus_tag="PKK36_03910"
     CDS             complement(6490..8388)
                     /locus_tag="PKK36_03910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNR93737.1"
                     /translation="MKHQASYGSWPSPIKPCDLAGACLGIQSPRIVDGTLFWLESRPA
                     ENGRCVVCSASGDAVPSGFSVRNRVHEYGGGAWTFMKGNLFFCNDSDRSIWRCRPKGP
                     PEIFIRSDGARYADLEPDPTRNRLICIREDGALSAVISADVLNRTEKVLFSGADFYSS
                     PRVSPDGKSLAWIEWNHPDMPWDSTVLCRADLDAAGNPGPAVRLAGGEKESVVQPQWS
                     PDGRLYFVSDRTGWWNLYRAGAAGDEPVASMDAEFAQPLWQFGMSNYAFVSDKYLAAA
                     FTRDGLWALALVDTRTMKMRVCDLPFTSYDHVRAGNGLVVFLAGSPREPGALVSMNAQ
                     SGQWTVVKRTTDIGINPDSLSEPEPVAFPSAGGRTAYGLFYPPRNESFEGPSGEKPPL
                     IVTVHGGPTAAVSPAFSLKTQFWTTRGIAVLDVNYAGSTGYGRVFREALYGQWGIADV
                     DDCCAGAKYLAGRGLVDPDRMVISGGSAGGFTALSTLAFRSVFSAGIIRYGISDCSAM
                     ARDSHKFEAHYLDRLIAPYPARRDVYEARSPLFHAEGISRPVIFFQGLEDRVVPPEQT
                     FAMYEALAARGVPAAYVAFEGEQHGFRKEENILRMVESELYFLSRIFNYHSADAPAPV
                     KIANLSAD"
     gene            complement(8385..>9548)
                     /locus_tag="PKK36_03915"
     CDS             complement(8385..>9548)
                     /locus_tag="PKK36_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR93738.1"
                     /translation="RDRLCLWTDIPTTVKQQPMQLEVESGPEESGDLLKKRIAGRILA
                     VEDDPLIARLLQRILEKKDYEVKTALSAEEAMEAVQGHLERFDLVLVDLNLPGEQGLD
                     FLKRLKRMDETVVGIVITGMASSENILASLRGGAFDFIAKPFRPEIVQQSVDRALQFR
                     RLLVENRNYALDLEAMVRLKSKDLTSALTRLQASYMFTLEAFAALVGAREQDTGQHSH
                     RVSEIARMISEKLALPDQEIEDITRGALLHDIGKIAIPDAILLKPGPLTDAERTIMQT
                     HAEVGYNILKSSEALCNVAELVYSHQERFDGSGYPRGLRGEEICIGARIFSVIDAYDA
                     MRAHRVYRKSMTVEKAVEELKRGSGSQFDPEVVKAFLALLPEIEAAGQWPEGE"
     assembly_gap    9549..9648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9649..10728)
                     /locus_tag="PKK36_03920"
     CDS             complement(<9649..10728)
                     /locus_tag="PKK36_03920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNR93739.1"
                     /translation="MNVLIIHSDRSVLDRLCQVVSSWDGSSATGIAEVDKIAGAAAGD
                     YDMIVAEYRDASPLPEILAGLWPWLKPVFVAASETPAPESCSGITVIAADDAEALLAC
                     REKCMDARRELLCDKPLSQLLRKSTTADFRRFCEGAIRAETLNEGLKELGTGISELAP
                     CAAVALLSLDSDDTRLLIHCMQEVPLLFVGQVREGMLNVCRHIADTQPDMQSIRMHAN
                     GMACEGEREFGLAVPYYVPAMHGSTLVGLFGVFPADGIAFTPDSAGRLYQVRHQLSLV
                     LASLSHTGRIAARDVLTDLFNRRGLNEEFERSWQLAKETECALGVITMDIDHFKTVND
                     TCGHAVGDIVLQEMADIVRWATRTGD"
     gene            10845..12026
                     /locus_tag="PKK36_03925"
     CDS             10845..12026
                     /locus_tag="PKK36_03925"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR93740.1"
                     /translation="MSSQAALIDSTAEYRLLRLCAGSPSHDPRIPELIQSVTDWNTVI
                     NMSIRHSVLPLLYEHLQPAGWDSIPDGARQRLQDLHYKCIVRNTRLLAELNRVLDLFS
                     GAGLQALPVKGPLLAIACYGALHRRRFDDLDLLLNAEAIPKARAVLMDAGYRSFVNLP
                     PAQMQAFIRAGWDVNLRQGDEEYSIDLSTGITPGFFCFRIPDAAIRENLYSLGYQGRS
                     YPAPGPELLLVMLAAHGSYHRWERLIWAADVRAVIRTSPELDMDRLRALARRTGAMRM
                     LELALCMAERLEPLPHPLREMIPDRGPVSRLADHPPSRLYYHFKVRERWRDRMCCLFR
                     YFFAPTFSDWKCISLPAFLSWLYYLIRPFRAVRDAIKKTRALMRGSGKYSTRQVSSQF
                     G"
     gene            complement(12007..13233)
                     /locus_tag="PKK36_03930"
     CDS             complement(12007..13233)
                     /locus_tag="PKK36_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93741.1"
                     /translation="MNILFLSHYFPPEGNAPASRVYEFSRRWAASGNEITVITSAPNV
                     PGGVVYPGYRNRFVRENQDGIRVQRVWTYLAPNKGTARRILNYLSYMFSAFFAGLAGR
                     RPDVVVATSPQFFCAWAGLLLAICRRRPFVLEIRDIWPESITAVEAMRNRAVLNVLEK
                     MEKMLYRRADAIVTVGEGYRQRLIEKGVPPGKISVVANGVTMKPGAIGPSASSELRAR
                     WCPDASFVCIYAGTIGMACGLDVVLRAADLLRRRGRENIHFLLVGDGAHREELESRSR
                     AMSLRGVIFTGLQKRSDVAAFLNMADACLVHLRKTDLFTTVFPSKLLDAAALKRPVIL
                     GVEGHAAQMVREADCGICIEPENAEQLADAVLKLAADPELCRRFGENGAAYANGHFNA
                     DRQAAEYMELLTRIVS"
     gene            complement(13230..14876)
                     /locus_tag="PKK36_03935"
     CDS             complement(13230..14876)
                     /locus_tag="PKK36_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNR93742.1"
                     /translation="MNRITRCLRTIADLRPVQIANRVWRHIYCPAPDLAPPPAFRKPL
                     QQWKRFAPRPASIGSDCTLELFHERHRLSGPDDWNNPAWPRLWLYNLHYFDGLCAENP
                     NEACGQLVERWLRDNPPGSGCGWEPYPLSRRIVNWIKYELGGNHLSELALHSLAVQVR
                     YLRARLEYHLLGNHLLANAVALIFSGLFFEGKEPARWRRKGVRLFERELKEQILPDGG
                     HIERSPMYHAIVLEDLLDIIQLHGVYGLDCPPSWAETAGRMLSWLRVMTHPDGGFALF
                     NDCAFNGASAPAVLETYADGLGIPRAPEGNGNAWLEHTGYLRVEEGNLVLFFDAGSVG
                     PRYQPGHAHADTFSVEMSIGRQRLLVDSGTSCYGISPERQRQRATPAHNTLSVDGCDS
                     TEVWSSHRAGRKARVTSIKFSQPPGGQGLAVLAVQDGFSRLRGVRMHSRRLDVRDGRI
                     VIEDTIDGSGPHELKLSFHFHPDIIVSKSRDVGYNLKNHEGLFLGTLQMDDALRSDVE
                     PATWHPEFGMTRNSVKLAGTCTVKLPARFTTVFELAEALK"
     gene            complement(14873..16054)
                     /locus_tag="PKK36_03940"
     CDS             complement(14873..16054)
                     /locus_tag="PKK36_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HNR93743.1"
                     /translation="MGPGELREEVCARIAALLPVARKSPFWRERLPERLPDFDAVAAL
                     PLTTKDDLRAVAIEQRSVQTSGCLRINTGGTSGSPLSFYIDRQAFAREWAHMHYIWQK
                     AGYLQTDIKLTLRGRIPGDQPLRYNAVHNEYVLHPSFPADRLAAAVEKIASRIRFIHG
                     YPSLIYSFLKYCDECRPEVIGRLRKSLKGVLLGSEFPAPVYRNFIDDVTGVPSVSWYG
                     HSEMALLALEQERGVYVPLQSYGYAEAVPASTAGEYRLVGTSYFNTASPFIRYDTGDL
                     VDAQVEDGLLKSFRITSGRVGDFVQDRHGCAISLTALVFGRHHALFDRAAHVQIRQTA
                     PGCVDLVVTPGPGQRMTPENVLAAFDSSGVDLDFRALVVDSPVRAPSGKIPLLIREWQ
                     T"
     gene            complement(16195..16965)
                     /locus_tag="PKK36_03945"
     CDS             complement(16195..16965)
                     /locus_tag="PKK36_03945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93744.1"
                     /translation="MQVYILARNEEDNIAACLQALPRSGLQVTVLDSGSTDGTLAAVS
                     RFSGVHVEPYAYTNHCEAYNWITAKKGASPYVMVLDADMKISDALMNEITNLIDAAAP
                     QVIKAPVTMCVDGVPLKHGSLYPPKPIVFKAGAAYFTADGHGERLLPELDVQTCRECL
                     VHDDRKPYEAFLESQCRYSRNFIGRETSGRLDWQDRLRAGTWLMMWIVPLYSWIICRG
                     FLSGRAGRAYALDRLIAEAIRCRFILSRKLHAKSSKEA"
     gene            complement(16986..18101)
                     /locus_tag="PKK36_03950"
     CDS             complement(16986..18101)
                     /locus_tag="PKK36_03950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93745.1"
                     /translation="MRILQVIGGLASSSGTTPAVLALSDRLANAGHGVDIFYVSGRTP
                     DTVMPSSPGVHVEGFPLSGPRIWAGSCALSRALMNRAGAYDVIHGYSLWLFPNIAVSR
                     ACRRARRPYIISPQGALDHWALSHHGLRKKIYADLIERRILSRAACIQCVTEHEVSLV
                     KNFGLSTKTAIIPNGVETGFFNPSAKQYPPEILFLSRLHPKKGLDILLPAFAAARREC
                     PGIRLTIAGGDGGSGYRKTIEALIAGLGLGDCVRLVGELRGGEKTAAFESSGMFILPS
                     RSEGHPLAALEAMERGLPVILTPQCYLPEAAKQGAGLIAGADVQEISQAIVRLASSEP
                     DRRKMGHVARRLIEEQFSADRIASLQAELYKEVAAKG"
     gene            complement(18098..19225)
                     /locus_tag="PKK36_03955"
     CDS             complement(18098..19225)
                     /locus_tag="PKK36_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93746.1"
                     /translation="MKNGRLRVWLLTNAPSPYQMEYLAALAKHMDLDVRLMRHAYADA
                     AWAGGAMPPEAHVMRGWGSERHRDELRLHPSALTEVWRGAHDVYVLSGVYTSPTFLMC
                     AWILHWRRKPWAVWFERPWPEDYRPAWARRASARSGLARVLRKRVLRWILRSATRVYC
                     IGSAAVNAYREWGVPENKLVFLPYVCDLEPFRSCRISEGDHIRFLYVGLLDQRKGVDL
                     LLDAFERLADEYAQVELILAGHGPVKKGCEGRRSARVRFAGHVERAELPALYGSADVF
                     VFPTRYDGWGVVLNEACGAGLPVIVTSAAGAAADLVEDGVNGIVISRDDPEGLYQAMK
                     YMVDHPESREAFGRRSLEKSSVVDMAPGVVRFLHAVKDMAG"
     gene            complement(19222..19956)
                     /locus_tag="PKK36_03960"
     CDS             complement(19222..19956)
                     /locus_tag="PKK36_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR93747.1"
                     /translation="MRERIKWLLFPGINLHTRQRYRILPRCLESAAPGGACLLDAGCG
                     NGMLSYRACLKGYRVKGISFKEGEVARCRKLFNGYLGIPEQRLQFEVMNLYDVEALNQ
                     QYDQIICTEVLEHLMRDEAVCRSLFALLKPNGVIHICCPNADHPYNRSFPLDPGENGG
                     HVRAGYTYESYRRLLEPIGFRIVEQRGLGGPVRQFFNSRIKRLQEKYGDAVAFPLFLL
                     SFLFLWLDREEARVPYTLYVKAVKPA"
     gene            complement(19949..>20789)
                     /locus_tag="PKK36_03965"
     CDS             complement(19949..>20789)
                     /locus_tag="PKK36_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93748.1"
                     /translation="ELLLYCREHGLTGVLDHATAPQGAEMKLVRAEQERFPGWVCAET
                     ESSVLEAYSRRQHEEDRLASIIICGCSFVKGMLADTGVDRDRIKVVPLGISIPPEMPV
                     VSHGGLNVLFVGSDGLRKGIGYLARAARLLGSSNIHFRAVGDLGLTSQGMNEIREDIK
                     VLGSVPRSAMREHWAWADVLVLPSVSETFGLVVLEAMASGLAVITTTSTCGPDVVRSG
                     VDGFVVPPRNAEALAEKLDWMASNPVLLSEMKRSARSHAGDFTLEKYGRRLAEAVRGG
                     SNA"
BASE COUNT         4554 a         6278 c         5767 g         4090 t
ORIGIN      
        1 gccttcctcc ccgtggcaag ccacggggaa cccggcgctt ggctttcttg gaagtatttt
       61 gacaaaaagt tccaaccatt ggaagttttc tgacgaaaag ttccagtcat tggaacttct
      121 gcgtattaga aaagttttcg aagacccggc gcagcgggga aacagccctt gacgggccag
      181 tcatggtccc tgaccggctg gcgacaaatc ggtctaaaac gctttacttt tgaagcgggg
      241 tcgcctatgt ttctgcgccg tttggacata gcttttattt aatatctgga gatgctcatg
      301 aaaaagaaga tcgccatcat ggatgggaac accgccgccg catatgtggc tcataaaacc
      361 aatgaagtat gcgccattta tccgattacc ccctcttcca acatgggtga actggccgat
      421 cagtggtcgg ccgaaggcaa gaccaatatc tggggcaccg tcccgcatgt ggttgaactg
      481 cagagcgaag gcggagccag cggagccgtg cacggcgccc tgcagggcgg cgcgctgacg
      541 acgacgttca ccgcctcgca ggggctgctg ctgatgatcc cgaacatgta caagatcgcc
      601 ggcgaactca ccagcacggt gttccacgtc tccgcccgca cgctggccac ccacgcgctt
      661 tcgattttcg gcgatcactc cgacgtgatg gccgcgcgcg ccaccggctg ggcctttctc
      721 gcctcggcct cgatccagga agccatggac atggccctga tcgcgcaggc cgccacactc
      781 gaggcccgcg tgcccattgt ccatttcttt gacggattcc gctcctccca cgagctcaac
      841 aagatcgagg tgcttggaga cgagaccatc cgcgagatga tcagcgagga tcttgtgctg
      901 gcgcaccgcg cccgtgcgct gacgcccgac cgccccgtgc tgcgcgggac cgcacagaat
      961 ccggacgtgt tcttccaggg ccgcgaggcc atcaacggct tctatactgc cgcgcccgcc
     1021 gttgtacaga aagccatgga caagttcgcg aagctgacgg gccgccagta tcatctcttc
     1081 gactaccacg gtgcaccgga tgcggagcgc gtcatggtga tcatggcttc aggcgccgag
     1141 accgcgctgg aaaccgtgga caccctgctg gaaaagggag aaaaagtcgg tgtgctgatt
     1201 gtccggctgt accggccctt ctctccgcaa cacctgctga aggtcattcc cgccaccaca
     1261 aaggcgctgg ccgtgctcga ccgcacgaaa gagcccggcg ccgacggcga gccgctctac
     1321 aaggacatcg tcaccgcgct ggcgcaggcc gcggcagatg gcaaactgcc cacgaagacg
     1381 atgccgaagg ttgtcggcgg ccgttacggc ctctcctcca aggagttcac ccccggcatg
     1441 gtcaaggccg tgctggacga gctcaaaaag gacaaaccca agaacagttt cacggttgga
     1501 atccatgatg acgtgacgca cagcagcctg gaatacaaga cattcctgac caacgcgccc
     1561 aaacaggtcc ggtgcatgtt cttcgggctg ggctctgacg gcaccgtggg cgccaacaaa
     1621 aactcgatca agatcatcgg cgaggaaacc gacaactacg cgcagggcta tttcgtttat
     1681 gactccaaga aggccggatc cagaaccgta tcccatctgc gcttcggccc gacgccgatt
     1741 cactcgacct atctggtcca gtccgcgaat ttcatcggct gccaccagtt cgttttcctg
     1801 gaacaaacgg atattctggg gcaggcggcg gacggcggcg tgttcctgct gaacagcccc
     1861 tatccgaaag aggaagtctg ggacaagctc cccgccaagg tgcagaagca gatcattgac
     1921 aagaagctca agttctatgt gattgacggc taccatgtcg ctgagcaggc cggcatgggc
     1981 acccgcatca acaccatcat gcagacctgc tttttcgcga tttccggcgt gcttcccaaa
     2041 gaagaggcca tcgagaaaat caaacaggcc atcaaaaaga cgtacgggaa gaagggcgaa
     2101 gatgtggtgc agaaaaacta cgtggccgtg gatgcggcgc tggcgcacct gcacgaggtc
     2161 gccgttccgg atcacgtcac cagcaggatt gacatgccgc cggtcgtggc ccctgaagcg
     2221 cctgaattcg tacagaaagt cacggccgaa atcatggccg ggcgcggcga tgatattccg
     2281 gtgagcgctt tcccggtgga cggcacttac ccgctgacca ccaccaagtg ggaaaagcgc
     2341 aacgtggcgc tttccgtccc gcagtgggat cccgacgtct gcatccagtg cggaacctgc
     2401 ggctttgtct gcccgcatgc ctgcatccgg tcaaagaaga gcacggaagc cgagctgaaa
     2461 agcgctccgg aaggattcaa gtccgccccg ctcaagggca aggacgctcc tgccgatgcg
     2521 cgcttcacgc ttcagatcgc cgtcgaggac tgcaccgggt gcggcctttg cgtgatggcc
     2581 tgccccgcga agaacaaaca gatcgaaggc cgcaaggcca tcaacatggc cgacaagctt
     2641 ccgatcatgg acgaggcacg cgccgcgttt aaatactttg acgaggttct tccggatacg
     2701 gatcgcagca ccctgaacct tgcgctgctc aaggatattc agtttgcgcg gccgctgttt
     2761 gaattctcgg gcgcctgcgc cggatgcggc gaaaccccct atgtcaagct gatgagccag
     2821 ctgttcggcg accgcatggt gattgccaat gcgaccggct gctcgtccat ctatggcggc
     2881 aacctgccca ccacgcccta cgcggtgaat caggacggcc gcggcccggc atggtcgaat
     2941 tccctgttcg aggataatgc tgaattcgga tacggattcc ggctggccta cgacaagcat
     3001 tccgaactgg cggccgaatt gctcaagaag ctgggcggcc agattggcga caaactggtg
     3061 gatgagctgc tgaattccga tcagtccact gaaggcggga tcgccgagca gcgccgccgc
     3121 gtggcggccc tgaaagagca gcttaaaggc atcaaatcgg acgaggcgag aaacctggcc
     3181 tcgattgccg acatgctggt gaaaaagagc gtctggattc tcggcgggga cggctgggcg
     3241 tatgatatcg gatacggcgg acttgatcac gttctggcca gcggacgcaa cgtcaacgcc
     3301 atggtgctgg atacggaagt gtattcgaac accggcggtc aggcatcgaa ggccactccg
     3361 cgcggggcgg tcgcgaaatt cgcggccggc ggcaagccga tggcaaagaa ggacctggcc
     3421 atgatggcca tgtgctacgg aaacatctat gtggcgcgca tcgccctcgg cgccaatccg
     3481 cgtcaggcga tcaatgcgct gctggaagcc gaggcctacg acgggccgtc gctcatcgtg
     3541 gcctattcgc actgcattgc gcacggcatc aacatgataa atggcctgga gcagcagaag
     3601 ctggcggtca agagcgccta ctggccgctc ttccattaca atccctctct cgcggctgaa
     3661 ggcaagaatc cgctggtgct ggactccaag gcccccgcga tgccgctgaa ggattacgca
     3721 tacaacgagg tgcgttacac catgctgacc aagtccaagc ccaacgaggc cgcaaggctg
     3781 atcgagctgg cccaggccga cgtggacaag cgctggaaaa tgtatgaaaa gtgggcccag
     3841 gaataatccc ttcttttatg ttgcacattt tcaaatctcg tatagcttgt tgctctgttt
     3901 tgcctgaaag acagattaga accttaatga aggagaaaag acgatgaagg taaagattga
     3961 cgaagacgcc tgcaccggct gcggactgtg ctgcgacacc tgcccggatg tgtttgaaat
     4021 gggcgacacc gtagcggctg tgaaagttgc ggaagtgccg gaagacgccg aagagacctg
     4081 ccaggaggct gtggacagct gcccggtgga atgcattacc gtcgttgaat aactgacaca
     4141 actgaaaacc ccctccctca tccggagggg gtttttctgt ttaatgacgg gtattgagaa
     4201 ctttccattt ccgctaaaaa gtgcattatg gtgcgcttga aatgaataac ggctgttcca
     4261 gagagttgca tgaagcactg cgcgatcagg cagcgggccg gacactgatc ttcaggcgcg
     4321 gaccggactg ttatgacccc ggcgatgttc tggagtacga agtcacgggc atacagccgt
     4381 gcacaaaggc acaggcccgg ctgaaggttg ataaattcct gggaggcggt ttcgcgggtc
     4441 aggtttatct ctgcactctc agcggcctgt caggcggaac aatcccgggc ctggtcgacg
     4501 ggcggcgcta tgccgttaag atcatgcagc cccccgacgg cgcggcgaca ctcttccgga
     4561 atttcattta ctggctcgct tttcagggac ccttcagcgc ccaggtgagc gaggcggcct
     4621 cgcgctccgg acttctctgg cagaagctct tccgcaaggc gggagagctg atattcgggc
     4681 ggtccacgtc cgttaaagat gtgtatgcgt ctttctgcga cgaaaatctg agagcctacg
     4741 gggaaatcac cgaatgggtg gagggccgca catggcggct ggagtctgat ccgcacatca
     4801 gaaagcgccg gcactgggaa accattgatt tagagaaaac cgcaagcgcg gaatacatcg
     4861 cgaaacggcg gttcatgcgg gacatggttg aactgatgca cgacatgggc gcgtcggaat
     4921 ttgcgcggca gtatgaatgg tctacaatga agagccagcc gaacgtcctg aaacggacgg
     4981 atgttgacga atccggaccg ggtggcggac tgtgcgccat tgatttccgg gcaggacttg
     5041 cccttcttcc cttcctcccg atgagcccgg gcgatttccg gctgatcctg cagggcttgt
     5101 tccgccgaaa atcccttgtg cagtttgacc gctgcaatct gcgcaagctt gacgaattca
     5161 tcgcaaggca tgccgggcac ttcaaagatg acgccgcgat gattgaggaa ctcaaggcct
     5221 gcgaccggaa gtaccgcaga tctctgccgg acatcacgca tcacggatgg aggcttctct
     5281 ttgaccgcga actgcggcgc gaagtgaggc gggggttgat ctgcgggtac agaatgaaca
     5341 ggctgacaga tgaggacttc gcgggaaaac tggaaaaatc ggccgggctt tttgcggttt
     5401 attatttcct gggcgctttt cctgttctcg gtcgaatcct gcggcagatc gccgggaatt
     5461 cgctataccg tcagcacttg aagcacgccg ccgggtcatg ggcatacatg caggaggcgt
     5521 tatctgcgcg gtgcgcgcgc ggcctggtat tgtggcttcg caaaggacgc gtcaatgaag
     5581 gacatgccct cgggctggca aagcaccccg gcctcttcct gatggagcgc tggactcttg
     5641 gacttttacc ggctggtctg caccgggccg tgagcgaacc gctctatgct ttccgtcgcc
     5701 tcaaaagctt tcttcttttt ctgaagaact tcatcagcaa tgccgcgtat cgggaaaaat
     5761 ggtttcttga tcagattgaa gacggcaggc gacagggaat gctcaccggg gaggaatata
     5821 cagaaattcg gaatcacgtt caggacccat tcatcgttaa atatctgaaa tgcctggggg
     5881 ttcatttcgc caccctgccg gtcacgcaga ttgtttcggt agcagtgggc ggcatcgtgg
     5941 cattcaaaat gctggcagcc ggacaaagcc ggacggacgc ctcgctggct tttgccgcag
     6001 tggttgccct gtttcaggtt cttccgatct cgcccggatc catctgccgc ggactctttg
     6061 tgctctatct gatgctgcgg gaacgaaatt tccgggacta catggtggcg atgccgcttt
     6121 catttgtgaa atacatcggc tatctggctt tccccctgca gatgacggcg acctatccga
     6181 agctggcgcg gttcatggcc gggcgctggg caacctcggc cgttcacatc atccctgtgt
     6241 ttggagagca gggcgcactg ctggagcact gggtttatga cctgttcttc aacgtgccgc
     6301 aggcattcgg catctgggcg agacctcgtg tcaggatgct gctgaacatc tggatggcgc
     6361 tggggcttgc gctcgggggc tggctcattg cgcatttcaa ctggccgctg aacgaaggtc
     6421 ctgctgtgaa cacaatcatc gcgactctgt gtttatttgc gctgccgcgc gtgctgttct
     6481 atccccttct caatcggcag aaaggtttgc gatcttcaca ggcgccggcg catccgccga
     6541 atggtaattg aatattcgcg acaggaagta cagctcactc tccaccatgc gcaggatgtt
     6601 ttcttccttt cggaacccgt gctgttcgcc ttcaaaggcg acataagcag ccggcacacc
     6661 gcgcgccgcc agcgcctcat acatcgcaaa cgtctgttcc ggcggaacca cccggtcttc
     6721 cagaccctgg aaaaatatca cgggacggct gatgccttcg gcgtgaaaaa gcggcgagcg
     6781 ggcctcatat acatccctgc gggcgggata aggcgcaata aggcgatcca gataatgcgc
     6841 ttcaaatttg tgcgaatccc gagccattgc agagcagtcc gatatgccgt accggataat
     6901 tcccgcggaa aatacactcc gaaaagccag cgtggacagg gcggtgaatc cgccggcact
     6961 gccgcccgaa atgaccatgc ggtccgggtc caccagtccg cggccggcca gatatttcgc
     7021 gccggcgcag cagtcgtcca cgtcggcgat tccccactgc ccgtagagcg cctcccggaa
     7081 gacccggccg tatcccgtgc tccccgcata gttcacatcc agcacggcga tgccgcgagt
     7141 cgtccaaaac tgcgtcttga gactgaatgc cggactgacc gccgccgtcg ggccgccatg
     7201 gaccgtgacg atcagaggcg gcttttcccc ggaagggcct tcaaagcttt cattgcgcgg
     7261 cggataaaaa agcccatagg cggtccgtcc tccggccgaa gggaaagcga ccggctccgg
     7321 ttcggacagg ctgtccggat tgattccgat atctgtggtt cgcttcacca cagtccactg
     7381 cccggactgc gcattcatcg aaacaagagc gccgggctca cgcggagatc cggccaggaa
     7441 tacgacgagg ccgttcccgg cgcggacatg atcataactg gtgaagggca ggtcgcacac
     7501 acgcatcttc atggtccggg tatccacaag agccagtgcc cagagcccat cgcgcgtaaa
     7561 agccgcagcc agatatttgt cggacacgaa ggcgtaattg ctcatgccga actgccacag
     7621 cggctgtgcg aattccgcat ccatggaagc gaccggttca tccccggcgg cccccgcgcg
     7681 gtacagattc caccagcccg tccggtctga aacaaaataa agcctgccgt cgggcgacca
     7741 ttgcggctgc acaacggatt ccttttctcc gcccgcgagc cgcacagcgg ggccgggatt
     7801 tcccgcggca tccaggtccg cgcggcagag cacggtggaa tcccagggca tgtccgggtg
     7861 attccactcg atccatgcca gacttttccc gtcaggactg actcttggtg acgaatagaa
     7921 atcggccccg ctaaatagta ccttttcagt cctatttaag acatctgcgg aaatgacggc
     7981 agacagggcc ccgtcctccc ggatgcagat cagacgattc cgggttggat caggctccag
     8041 atcggcatac cgggccccgt ccgagcggat aaagatttcc ggaggccctt tcggccggca
     8101 gcgccagata gagcggtcgc tgtcgttgca aaagaacaga ttccccttca tgaaggtcca
     8161 ggctccgcca ccgtattcgt gcacccggtt ccggacactg aacccggagg gaaccgcatc
     8221 cccggaggca gaacagacaa cacagcggcc gttttcagca ggccggcttt ccagccagaa
     8281 gagtgtcccg tccacaattc ggggtgactg aatcccgaga caggccccgg cgaggtcaca
     8341 gggttttatc ggagatggcc agcttccata tgaagcctga tgcttcattc tccctcaggc
     8401 cactggcccg ccgcctcgat ttccgggaga agagccagaa aggccttcac aacttccgga
     8461 tcaaactggg agccgctgcc ccgcttgagc tcctccacgg ccttttcaac agtcatgctt
     8521 ttgcgataaa cccgatgagc tcgcatggca tcataggcat caatcacaga gaagattcgc
     8581 gcaccaatgc atatttcctc gccacgcagg ccgcgcggat agcccgagcc atcgaagcgc
     8641 tcctgatggg agtaaacgag ttcagcaaca ttgcagagcg cttcactgga cttcagaatg
     8701 ttgtatccca cctcggcatg cgtctgcatg atcgttcgtt ctgcatccgt caggggcccg
     8761 ggtttgagca ggatggcatc cgggatggca atcttcccga tgtcatgcag cagcgccccg
     8821 cgggtgatat cctcaatttc ctgatcggga agagccagct tctctgaaat catgcgggcg
     8881 atttccgaga cgcggtggct gtgctgtccc gtgtcctgct cccgcgcgcc aaccagagcg
     8941 gcaaaagcct caagagtaaa catgtaggat gcctgcaaac gagtcagggc acttgtcagg
     9001 tctttgctct tcaaccggac catggcctca agatccaggg cataattcct gttctccacc
     9061 agaagccggc ggaactgaag ggctctgtca accgactgct gcacgatctc cggcctgaaa
     9121 ggcttggcta tgaagtcgaa agcccctccc cgcaggctgg caaggatgtt ctccgatgag
     9181 gccatgccgg tgatgacaat ccccacgacg gtctcgtcca ttcgcttgag gcgctttaaa
     9241 aaatcaagac cctgctcgcc cggcaggttg agatccacaa ggacaaggtc aaacctttcc
     9301 aaatggccct ggactgcctc catcgcctct tccgcagaca gggccgtttt gacttcatag
     9361 tcttttttct caagaatacg ctgcaggagg cgggcaatga gcggatcatc ttccacggcg
     9421 aggatacggc ccgctatgcg cttcttcagc agatcaccgg attcttccgg cccggattca
     9481 acttccagct gcatgggctg ctgcttcaca gtggttggga tatcagtcca caggcacagg
     9541 cgatcgcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cccccgttcg
     9661 ggtggcccag cgcacaatat ccgccatttc ctgcagcacg atatcgccca ccgcatgacc
     9721 gcaggtatca ttgaccgtct tgaaatggtc aatatccatg gtaatcacac ccagagcgca
     9781 ctccgtttct ttagccagct gccatgaacg ctcgaactcc tcgttcaggc cccgacggtt
     9841 gaagagatcc gtgagcacat cgcgggcggc gatgcgtcct gtatgtgaaa gtgaagccag
     9901 cacgagcgac agctgatgac gcacctgata gagacgacca gcgctatcgg gcgtgaatgc
     9961 gatcccgtct gcggggaaaa caccaaacag gcccacgagt gtggacccgt gcatggccgg
    10021 aacataataa ggaacagcca gtccgaattc cctttctcct tcacacgcca tgccgtttgc
    10081 gtgcatgcgg atgctctgca tgtcgggctg tgtatccgcg atatgccggc aaacattcag
    10141 catgccctca cgcacctggc cgacaaacaa caggggaact tcctgcatgc agtggatcaa
    10201 caggcgtgtg tcatccgaat ccaggctgag cagcgcaacg gcagcgcaag gggccagttc
    10261 tgaaatacct gtgcccagtt cctttaaccc ctcattcagg gtttcggccc ggatagctcc
    10321 ttcgcaaaaa cgacggaaat ccgccgttgt cgacttacgc agcagctgag aaaggggttt
    10381 atcgcacaac agttctcggc gggcgtccat gcatttttca cggcaggcca gcagggcttc
    10441 agcatcgtct gcggcaatga ctgtgatgcc ggaacagctt tcgggagccg gcgtctcaga
    10501 agccgctaca aaaacaggtt tcagccacgg ccacagaccg gccagaattt caggaagagg
    10561 ggatgcatcg cgatattcgg caacaatcat gtcataatcg ccagccgccg ccccggcaat
    10621 cttgtcaacc tccgcaatgc cggtcgccga gctgccatcc cacgaagaaa ccacctgaca
    10681 cagccggtcg agaacagacc gatcggagtg aataatcagc acattcactg cactcccggt
    10741 ctccgatggc aattctcgat ttatcattcg tttataatac cattcatctg gtttccttgc
    10801 aaaataatgt atagagtttg agcggatttc aggcagacaa acgaatgagc tcacaggcgg
    10861 cattaattga ttcgactgca gaatacaggc tcctgcgctt gtgcgcggga tccccgtcgc
    10921 atgacccgcg cattccggaa ctgatccagt ccgtaacaga ctggaataca gtcattaaca
    10981 tgtccatccg gcattctgtt ctgccgcttc tgtatgagca tcttcaaccg gccggatggg
    11041 attccatacc agacggcgcg cgccagcggc tgcaggatct tcattataaa tgcatagtcc
    11101 ggaacacccg ccttctcgct gaactgaacc gggtgctgga tcttttttcc ggcgccggac
    11161 tgcaggcgct gccggtcaag ggaccgcttc tggccatcgc atgctacgga gcccttcaca
    11221 ggcgtcgctt tgatgatctt gatcttcttc tgaacgcgga agccattcca aaagcccgcg
    11281 ccgttctgat ggacgcgggg taccggagct ttgtcaatct tccgccggcg caaatgcagg
    11341 cctttattcg ggccggatgg gatgtcaatc tccggcaggg agacgaggaa tactcgattg
    11401 atctcagcac gggcatcacg cccggcttct tctgtttccg cattccggat gctgcaatca
    11461 gagaaaatct ttattctctg ggatatcagg ggcgcagtta tccggcaccg ggaccggaac
    11521 tgctcctggt catgctggcc gcacacgggt cctaccaccg atgggaaaga ctgatctggg
    11581 cggcagatgt gcgggccgtc atccgaacca gtccggaact tgacatggac aggcttcgtg
    11641 cgcttgcccg cagaacaggg gccatgcgca tgctcgaact ggcgctctgc atggctgaac
    11701 gactggaacc cctcccccat cctcttcgtg aaatgatccc ggatcgcggg cccgttagcc
    11761 gcctggccga tcatcctccg tcccggctgt actatcactt caaggttcga gaacgatggc
    11821 gggaccgaat gtgctgtctg ttcaggtatt tcttcgcccc gacattcagc gactggaaat
    11881 gcatcagtct gccggccttt ttgtcatggc tgtactatct gattcgcccg ttccgggcgg
    11941 tccgagacgc cataaaaaaa acccgcgcat tgatgcgcgg gtccgggaaa tattcaacgc
    12001 ggcaggtcag ctcacaattc gggtaagcaa ttccatatac tccgcggcct ggcgatccgc
    12061 attgaagtgc ccgtttgcat aagcagctcc gttttctcca aatcggcggc agagctccgg
    12121 atcggcggcc agcttcagca cggcatcggc cagctgctcg gcattctcgg gctcgatgca
    12181 gattccgcag tcagcctccc tgaccatttg agctgcatgc ccctccaccc cgagaattac
    12241 cggacgcttg agagcagccg catccagcag tttggacggg aacaccgttg taaacaggtc
    12301 tgtttttctc aggtggacaa gacacgcgtc cgccatattc aaaaatgcag ccacatcgct
    12361 tcttttctgc agtccggtga agatcacgcc gcgaagactc atggcccggc ttcgggattc
    12421 cagctcctcc cgatgagccc cgtctcccac cagaaggaaa tgaatattct cccggccccg
    12481 gcggcggagc aaatccgcgg cccgcagcac aacatccagg ccgcatgcca ttccgatggt
    12541 gccggcatag atgcacacaa aggacgcatc aggacaccac cgggcccgca gttcagacga
    12601 agcggacggg ccaatagccc cgggtttcat ggtgacgccg tttgcaacga cggatatttt
    12661 tcccggtggg actccctttt caatcagccg ctgacgatat ccttcaccca cggtgacaat
    12721 cgcatccgcg cggcggtaga gcatcttttc cattttctca agcacattca aaaccgcgcg
    12781 gttccgcata gcctcaacgg ctgtgatgga ttcgggccag atatcacgaa tctccaggac
    12841 aaaaggacgg cggcggcaga tcgccagcag caggccggcc cacgcgcaga agaactgcgg
    12901 ggaagtggcg acaacaacat ccggacgccg tccggcaagc cctgcgaaga aggccgaaaa
    12961 catataggac agataattca gaatccgccg tgccgtgccc ttgttaggcg ccagatatgt
    13021 ccaaaccctc tgcacccgaa tgccatcctg attctcgcgc acaaagcggt tgcggtagcc
    13081 gggatagacc actccgccgg gaacattggg cgcactggtg atcacagtga tctcgttccc
    13141 cgaggcggcc cagcgcctgg agaattcata gacccttgag gcgggcgcat tgccttccgg
    13201 cgggaaatag tgcgacagga aaaggatgtt catttgagcg cttccgccag ttcaaatacg
    13261 gtggtgaacc ttgccggaag tttaacggtg caggtccccg caagcttcac ggaatttctg
    13321 gtcatcccga attcaggatg ccatgttgcc ggctccacgt ccgagcgcag ggcgtcatcc
    13381 atctgcagcg tacccagaaa caggccctcg tgatttttga gattatagcc cacatcacgg
    13441 cttttcgaca caatgatgtc cggatggaaa tggaatgaaa gcttcagctc atgcggaccc
    13501 gaaccgtcaa tggtgtcttc gatgacgatc cggccatccc gaacatccag tcttcggctg
    13561 tgcatgcgca ccccgcgcag ccggctgaat ccgtcctgta ccgccagcac ggcaagcccc
    13621 tgtcctcccg gaggctggga gaacttgatg gaagtcacgc gggccttgcg ccccgcgcgg
    13681 tggctgctcc agacttcggt ggagtcgcag ccatccacgc tgagtgtgtt gtgcgcgggg
    13741 gttgcgcgct gacgctgccg ctcggggctg atgccataac aggacgttcc ggaatctacg
    13801 agaagccttt gtctcccgat ggacatctca acagagaaag tgtccgcatg agcgtgaccc
    13861 ggctgatacc gggggcccac ggaacccgca tcgaagaaca gcacgagatt tccctcttcc
    13921 actctcaaat atccagtgtg ttcaagccat gcattgccgt tcccctcagg ggcccgcgga
    13981 atgcccagac cgtcggcata ggtctccagc accgccggcg cggaggcccc attgaaggcg
    14041 cagtcattaa acagtgcaaa tcccccatcc ggatgcgtca ttacccgcag ccacgaaagc
    14101 atgcggccgg cggtttcggc ccaggacggc ggacagtcca gaccgtacac gccatgaagc
    14161 tgaatgatat ccagcagatc ctccagcaca atggcgtgat acatcggaga ccgctcgata
    14221 tgtccgccgt cgggaagaat ctgctccttc aattctcgct cgaacaggcg cactcctttt
    14281 cgccgccagc gggctggttc ctttccttca aagaacaggc cggaaaatat gagcgcaacg
    14341 gcgttggcca gcagatgatt tcctagcaga tgatattcca gccgagcacg aagataccgc
    14401 acctgaacag ccagactgtg gagcgcgagc tcagacagat ggtttccgcc caactcatat
    14461 ttaatccagt ttacgattct acgcgaaagc ggatacggct cccatccgca gccgctgccc
    14521 ggaggattgt cacgcagcca cctctcaacc agctggccgc atgcttcatt gggattttcc
    14581 gcgcagagtc cgtcaaaata gtgcagatta taaagccaca ggcgcggcca tgccgggttg
    14641 ttccagtcgt ccgggccgga cagccggtga cgctcatgaa aaagttccag tgtgcaatcg
    14701 cttccgattg aagccggtct gggggcaaag cgcttccact gctgcagggg ctttctgaat
    14761 gccggcggcg gggccaggtc cggcgcgggg cagtatatgt gccgccaaac gcgattagca
    14821 atctgcaccg ggcgcagatc agcgatcgtt cgcaagcacc ttgtgatcct gttcatgtct
    14881 gccactccct gatgagcagc gggatttttc ccgagggagc ccgcaccgga ctgtccacca
    14941 ccagcgcacg gaaatccaga tcaaccccgc tcgaatcaaa tgccgcaagc acattctccg
    15001 gcgtcatccg ctgtcccggg cccggcgtca ccacgagatc cacacaaccc ggagcggtct
    15061 gccggatctg gacatgagcc gcccgatcaa agagagcatg gtggcggcca aaaaccagcg
    15121 ccgtcaaact gatcgcgcag ccatgtcgat cctgcacgaa atcgcccacg cggcccgatg
    15181 tgatgcgaaa ggatttcagg aggccgtctt ccacctgcgc atccaccagg tcccctgtgt
    15241 catagcggat aaaaggcgat gccgtgttga aatacgatgt tcccaccaga cggtattcgc
    15301 cggccgtcga ggccggaacg gcctcggcat atccatagga ctgaagcggg acataaaccc
    15361 cccgctcctg ctccagggcc aggagggcca tttcagaatg cccgtaccac gaaacactgg
    15421 gcaccccggt cacgtcatca ataaagttcc tgtacaccgg agccgggaac tccgaaccca
    15481 gcagtacgcc tttcagtgat ttccgcagac ggccgataac ctccggccgg cattcgtcgc
    15541 agtatttaag aaacgaatat atcaacgatg gatacccgtg aatgaatcga atgcgggatg
    15601 caattttttc cacggcggcg gccagccggt cggccgggaa tgaaggatgg agcacatatt
    15661 cattgtggac cgcattgtag cggagcggct gatcacccgg gatgcgtccg cgcagggtca
    15721 gcttgatgtc cgtctgaaga tatccggctt tctgccagat gtaatgcatg tgagcccact
    15781 cccgcgcaaa agcctgccga tcgatgtaaa aagaaagcgg cgaacccgaa gttcctcctg
    15841 tattgatacg caagcagccg gaggtctgca cagagcgctg ttcgatggcc acggcccgca
    15901 gatcgtcctt tgttgtcagc gggagagctg cgacagcatc aaaatccggg agcctttccg
    15961 gaagcctttc ccgccagaac gggctctttc ttgcaacggg aagaagcgcc gcgatgcgcg
    16021 cgcacacttc ttcccggagt tctcccggac ccatttgcag gacattccgc aaatcgcgcc
    16081 gggcgcgact gtaggcaggt cccagccgaa gggaaaacgg caccgctgcg agccaaggcc
    16141 cgagacctgg cggaattttt tccgccgcag ttttcatcct ggccagatga acgctcatgc
    16201 ttctttggac gactttgcgt gcagtttgcg ggacagaatg aaccgacagc gaatggcttc
    16261 tgcgatcaag cggtcaaggg cataggcccg gccggcccgg ccggaaagaa agccccggca
    16321 gatgatccat gaatacagcg ggacgatcca catcatgagc catgtgcccg cccggagacg
    16381 gtcctgccag tccagacggc cggacgtttc ccggccgatg aaattccgcg aatatcggca
    16441 ttgcgattca aggaaggcct cataaggctt gcgatcgtca tgaacgagac actcacggca
    16501 ggtttggaca tccaactccg ggaggagcct ttccccgtgt ccgtcagccg taaaataggc
    16561 tgcgccggcc ttgaacacaa tgggcttcgg gggataaagc gacccgtgct tcagcggaac
    16621 gccgtccaca cacatcgtga ccggcgcctt tataacctga ggtgccgccg cgtcaatcag
    16681 gttcgtgatc tcattcatca gcgcatcact gattttcatg tccgcgtcca gcaccatgac
    16741 atagggggaa gcgccttttt tcgcggtgat ccagttatat gcctcgcagt ggttcgtgta
    16801 ggcatagggc tccacatgaa cacctgaaaa tcgtgacacc gcggcgagtg tcccgtcggt
    16861 tgaacctgaa tccagcaccg tcacctgcag gccggaccgc ggcagggctt ggagacaggc
    16921 tgcgatgttg tcttcctcat ttctcgccag aatatatacc tgcatgttcc ttggcatgat
    16981 gatattcaac ctttcgccgc aacttcctta tacaattctg cctgaagaga agcgatcctg
    17041 tctgctgaaa attgttcctc aatcagcctg cgggcaacat ggcccatttt ccggcgatca
    17101 ggctccgatg acgccagccg gactatggcc tgggatatct cctgcacatc cgcgcccgcg
    17161 atcagtccgg caccctgctt tgcagcctcc gggagatagc attgaggcgt gagtataaca
    17221 gggagccccc tctccatggc ctccagcgcg gccaggggat gcccttcgga acgcgacggg
    17281 agaataaaca taccggatga ctcaaatgcc gccgtcttct cccctcccct caactccccc
    17341 accagccgca cacagtcacc caggccaagc ccggcgatca atgcctctat ggtttttcga
    17401 tatccacttc ccccatcgcc tccggcaatt gtcaggcgaa tcccgggaca ttcgcggcgc
    17461 gcggcggcaa aggcggggag tagaatatcc aggccctttt tcggatgcag acgggaaagg
    17521 aacagaatct ccggcggata ctgttttgca gaaggattaa agaaaccggt ctcgactcca
    17581 tttggtatga tggccgtttt cgtcgagagg ccgaagtttt tcaccagaga tacctcatgc
    17641 tccgtcacgc actggataca ggccgcgcgg ctcaggatcc ttcgctcgat caggtcagca
    17701 tatatttttt tgcgcaggcc gtgatgactc agcgcccagt ggtcaagggc accctgcgga
    17761 gaaatgatat agggccttct ggcacgccgg caggcacgcg acaccgcaat gttcggaaac
    17821 agccacaggc tgtagccatg gattacgtca tacgctcccg cccgattcat cagggcccgg
    17881 gacaatgcgc aagagccggc ccatatcctg ggaccgctca ggggaaagcc ttcgacatgc
    17941 acgcccgggc tggatggcat tacggtatcc ggagtgcgcc cactgacata aaagatatcc
    18001 actccgtgcc cggcatttgc caaacggtca gacagggcca gaaccgcagg cgtcgtcccg
    18061 ctggatgaag ccaacccccc aatgacctgt agtattctca tccagccatg tccttcaccg
    18121 cgtgaagaaa gcggaccacg cccggggcca tatcaacaac agacgatttt tccagagaac
    18181 gccggccaaa tgcctcgcgg gactctggat ggtcaaccat gtatttcatg gcctgataca
    18241 atccttcggg atcatcgcga ctgataacaa tgccatttac gccgtcttcg accagatcgg
    18301 cggccgctcc ggcggccgat gtgacaatca cgggcagacc cgcgccacag gcctcattga
    18361 gaacaacgcc ccatccgtca tatcgtgtgg gaaacacaaa aacatccgcc gaaccgtaaa
    18421 gagccggaag ttctgcacgt tcgacatggc ccgcaaaccg cacccgggcc gaccggcggc
    18481 cttcacagcc cttcttcaca gggccgtggc cggcgaggat caactcgacc tgtgcatatt
    18541 catccgcaag tctctcaaat gcatcaagca gcaggtcaac tcccttgcgc tgatcaagca
    18601 aaccaacata gagaaatctg atatgatctc cctcgctaat gcgacatgag cggaaaggct
    18661 ccaaatcgca cacatacggg aggaacacca gtttgttttc aggaacgccc cattcacggt
    18721 atgcgttcac agccgccgaa cctatgcaat acacccgtgt ggcgctcctg agaatccagc
    18781 gaagcactct tttccggagt acgcgagcca acccgcttcg cgccgaggcg cgacgggccc
    18841 atgccggccg gtagtcttcc ggccaggggc gctcaaacca gacggcccac ggtttccgcc
    18901 gccagtgaag aatccatgcg cacatcagga aggttggact ggtatacacc ccggaaagca
    18961 catacacatc atgtgcgcca cgccacacct ccgtcagggc cgaaggatgc aggcgcaact
    19021 catcacgatg ccgctcgctc ccccagcccc gcatgacatg cgcttccggc ggcattgccc
    19081 cgcccgccca cgccgcgtcg gcataggcat gcctcatgag acgcacatcc aaatccatat
    19141 gcttcgcaag cgcggccaga tactccatct gatatggact gggagcattg gtaaggagcc
    19201 agactctcaa tcgcccgttt ttcatgccgg cttcaccgcc tttacgtaca gtgtataagg
    19261 aacgcgggcc tcctcacgat ccagccacag gaaaagaaaa gagagcagga aaagcgggaa
    19321 cgcaacagca tctccatatt tctcctgaag ccgttttatt cggctgttga aaaactggcg
    19381 cacgggccct ccaaggcccc tttgttccac aatgcgaaat cctatgggtt ccagaagacg
    19441 cctgtagctt tcataggtat atcccgcccg aacatgtccg ccgttttccc cggggtccag
    19501 agggaaactg cggttgtagg gatggtccgc gttgggacag cagatatgaa taacgccatt
    19561 cggctttaga agggcaaata aactgcggca cacggcttca tccctcatga gatgttcaag
    19621 aacctccgtg catatgattt ggtcatactg ctgattgagc gcttcgacat catacagatt
    19681 cataacctcg aactgcagtc gttgttccgg gatgcccaga tacccgttga aaagttttct
    19741 gcagcgcgcc acctccccct ccttgaaact gattcccttg acgcgatatc cttttaaaca
    19801 ggcccgatag gacagcattc cgttgccgca ccccgcatcc agcaggcatg ctccgccggg
    19861 tgccgcggac tcaagacagc ggggcaggat ccgataacgc tgccgcgtat gaaggttgat
    19921 tcctgggaaa agaagccatt ttatgcgttc acgcatttga acctcctctt acggcctccg
    19981 caagccgtcg gccgtatttt tccagcgtga aatcacctgc atgtgaacgt gcactgcgct
    20041 tcatttcaga cagcagcaca ggattcgagg ccatccagtc cagcttctct gcaagagcct
    20101 ctgcattgcg aggaggaaca acaaatccgt ccactccact gcgaaccaca tccgggccgc
    20161 aagtgcttgt ggtggtgatc acagcaagac ccgaggccat cgcttcaaga acaaccagac
    20221 cgaaggtttc gctgaccgac gggagaacca gcacatcggc ccaggcccag tgttcacgca
    20281 ttgcagaacg cggaacagag ccgagcactt taatgtcttc acggatctca ttcatcccct
    20341 gcgaggtcag gcccaagtct cccactgccc gaaagtgaat attcgaactc ccgagaagcc
    20401 ttgccgctcg ggccaaatat cctatgccct tgcgcaggcc gtcactgccg acgaaaagca
    20461 cattcagtcc gccatgtgac acgaccggca tctccggcgg aatggatatc cccagcggga
    20521 caactttaat ccggtcgcgg tccacgcccg tgtccgccag catgcctttt acaaacgagc
    20581 agccgcaaat aatgatggac gccaaacgat cttcttcatg ctgcctccgg ctgtaagcct
    20641 cgagcaccga ggattccgtc tctgcgcaaa cccagccggg gaagcgctcc tgttctgcgc
    20701 gaaccagctt catttcggct ccttgaggcg cggtggcatg gtcaagaaca cctgtcagcc
    20761 cgtgttcccg gcagtagaga agaagctcc
//
LOCUS       DAOQWR010000030        20591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_2957_length_20591_cov_5.306194, whole genome shotgun sequence.
ACCESSION   DAOQWR010000030 DAOQWR010000000
VERSION     DAOQWR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20591
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_20591_cov_5.306194"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKK36_03970"
     CDS             complement(<1..417)
                     /locus_tag="PKK36_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR93749.1"
                     /translation="MHEAHPLETSGRTLDHAAAVYDVLSPLMTFGLERKYRKAAIRLL
                     ELGGSEAVLDVGCGTGTLTMEIALVLNRGGRITGVDAAPKMIEVAQRKAAKVSNIAYA
                     AALAENLPYPNESFESAVSTFFFHHVNYGLKVRSLNE"
     gene            complement(410..1417)
                     /locus_tag="PKK36_03975"
     CDS             complement(410..1417)
                     /locus_tag="PKK36_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93750.1"
                     /translation="MKKVWNGGHILRTALEEQRRSIASVYDHARALVETPPGTEPDFQ
                     TSLESIPISFYSARKNIFSTLFQSAYQLLDIAPARRHLYGQLIHLFRIWVTSADNLLD
                     NEDKIVLPVRLPGVSHVMRQVVSLMAADRVMNCILDEAVQAGVISGDQGRELSRRSLQ
                     VLLPSAAQEASEEGGVTDRPDPEYVLRTIHRLKTGLLFHIPFLGPEVVEDSIDTSKMQ
                     RVKGALLDFGLGCQLLDDVRDMARDYVERRHNYCLSVMARDCPEPYRRLAAAGLKAGD
                     RLYQDQPMVVLPAVRRGLGLLSGALAALGEEGLGIPGRHAESMSRRMLAMLDLGDLQY
                     A"
     gene            complement(1601..2182)
                     /locus_tag="PKK36_03980"
     CDS             complement(1601..2182)
                     /locus_tag="PKK36_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR93751.1"
                     /translation="MSFTLEVGMTAPDFSLPATDGNKYSLAGFSDAEGLVIFFTCNHC
                     PYVLGSDEVTRATALKFRDCGVRFVAINSNSRNTYAEDDFEHMVARMNEQKFPWVYLH
                     DESQNVARAYGALRTPHFFVFDKARRLVYTGRGVDNPRDTSKMTVNDLERALAELTSG
                     KPVSVPVTNPIGCNVKWEGRDAHWMPPEACDLV"
     gene            complement(2200..2832)
                     /locus_tag="PKK36_03985"
     CDS             complement(2200..2832)
                     /locus_tag="PKK36_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93752.1"
                     /translation="MGGKLCPKIILEGTRLTFKTDVAFTLNEHPRIVGPRRYRYHSPL
                     ISAEWCAFTNWPWGRGLINYEPEEEERAMETYEAWMRLLELQKYYSWIIDRFHISTQA
                     FQHQARGAAPNFQWLEKRLKAVGFVIVFLNRSPESFEAARRERLKVSGKPDQYDDLKM
                     FIREQGLMRDLVFKSCLPVMDLDISDNDVVAAVDKIADWMENNGYLYANY"
     gene            complement(2832..10553)
                     /locus_tag="PKK36_03990"
     CDS             complement(2832..10553)
                     /locus_tag="PKK36_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93753.1"
                     /translation="MKHVPAYFFGEYGSDTSDAGYCGGAQVQFNITRGFSDWDNHRDT
                     VFNTEAPRDDAMMFGEHLGEPPGYGDYLGRGMRLVDNPLRNELNNRLGSMWNGLNGYD
                     SPGAGGFSPDTGVMHAQSHDNDYAARRELQHAFYFTRAGLGLLYTDGNHHAETLGESG
                     GAFPRHANTSFLGQWGDVRVPNLLYIHEQFARGYQKGVWSDADYVAYERLDWRQGGST
                     DGDKVCMLFMLNDNYSSGQARDIRNNISYSHTPMGDNAYLYNYSSYGGGFYTWASDLD
                     SVIVPAGGYFAFSWKNPDPSSMWTCFGGSPVTIYQNGAQAGTLSYTRKDGPDGDANFN
                     PYSLPDTNSTDYAYTMTVPRITSPTNVNFVAHVDGSAANVLLKLDGGMDLNGANHAGG
                     DPRDNPPALSDDIFVGYEQPSFVSRVHREKFAAVNSASNKIGSAGAETYHCTIGSGSM
                     TINPSDGVNDGNNSETAFFVWHSPSATTDQGALHFSPAPAQAAGSNLTIWVKTGDPNN
                     INHVCLYYTIDGATWPEGAGGMGAGNTRTIQFSFDHSDSGDSKDWWKATVPAMSSNTV
                     FRYKIGGYRQQDGGTYAPWNIVWPADANAVALKTTRMGTWQVTNFSPSKVSHRVHADY
                     GTMRTGLVEGFHMISARAFLERDNRAALYNTFKQTFYYDAMCPTGFIQYPANNGDSVA
                     GQQYGVVVRTDPTVTEVWFHIADWDTNNDDVVTGVANGNGSGFEPYTDGNASGSYDTG
                     EAFVDINGNGVWNSNIGETWVRAYAATPSDMSTVYPKEWRFNYNNIPSGGSNATIKVR
                     LRELSSVARPAFTNHTDATGHFTTLQRTVYTWGPDVRMFIAWPQQNGDYVGEGYVMKA
                     YFTKALADGLSSNDLIDRFLIKIQSSESGKTDGGVAQSRDTYSIHWNETDQYHALSMP
                     LGNLYNGVTDWLHGIEVTFDRDSLPDLVANRLVRATPAAEAPYIAITEPPEYGPDGRP
                     HQIIIPDVQNPSNEQRRTTVRIETGTNAASVSLSFTYAPDGYLAQGGGVSSLSSTNSG
                     SSILWECLWTNLLAGQYGLLATVSTAGGVSNSALRNATVVKREWVNSNTNDLDDDDDG
                     LNDVNEATSVPLPNARETDPEPNPETWDNGEVHVHFAYGRSLPQSPDSDGDGLPDGLE
                     CGWRNPSSNTDTNADTNGDGWKNFCSDYDPPFYNTLDNYGKVPGVNSQSEGGDRAKQL
                     YGSMTDPANPDTDGDGLPDGIEDANRNGWVDGDGEIIDPASPPSLGRSWPNGEMDTGE
                     TWTETDPGNSDTDGDGLSDGYGEDRDFNGRIAGDSNSNRIYNAGEAWTETDPLKADTD
                     GDGLPDGWESQYNLDPLDNGTDSLRTASGSDGDAENGAAGDPDGDGFDNATELVNGTN
                     PRWADTGVPPPEGSITIGRGPAIGVINGATNYQEFSDWSADDLIALDDYNNGGSQAVD
                     IYRAWDGFDTSRDIVAFYAHDGGSAGSGGDGLFHFRIDLLDLQPMAEDGYLDLYVIID
                     MNSPGVGEASLPDSADMVTEMKWEAAVAVYGGNNGRVYVDTQRANNSVSALDDLSPYG
                     VVGRDQNTANGFGQAYFNSELDAVEFSISRQALLDAGWNGLNPGQLNYQVFTMKDGTQ
                     NSPVGPGDIGGRNDLRDSITDDWVCSDYWRDQGNIQLNGKLYSWVGRNAANDYGKAAK
                     VAMLVHGNQHITPGSEIQNLINDGSGAGYHRALEIHDVFDQPLNLHVTPTLASAIQWA
                     RADTNAGKPWLDGPALNERIRSMILTNTVKLLGSTFSDHILPYFTPEFNNDNAELARD
                     HLKTIYSFTNSDSTVFWPPERVLDADVLGKITNMGYRFTLIDQNTHMWNWIGRTPALS
                     DDGYRINKINGVYCFVINNSAGDYRFVNTDGGLSIPLREMLNRRARSDTQDQVATILS
                     NWEDFGNSAQADAYDRNIRWLANHPWIQLVALEDIAAGRIDITGDGGGDYWGHLDRGA
                     STPPKTGHIWVQHATQNNYDNWYVGSAQEEGLQNKVFEIRPGTNLPAAYGMLYYNGIV
                     SSAWEKVKSVSDDSLARLARAAIHASVFETAFHEEDNNDLRRFSTGDFIYPDTTYNSL
                     IGFAKNAQSQTRMAAVYGRVDQWAAAAAGLTNTTAALEDVDLDGETECLLYNGRVMFI
                     MERTGGRMIMAFARDSAGSVFPVAGTPMAYSGSETELEGTANVATNGSVEAFRTSCLK
                     DWWAGTTNYVNMMYAAVNWTNGWRLTSADGKITKTLTLGPKTNRLEVAYSISETLNGG
                     VLYVRNGLSPDTEGLMLYGQQILSDPAVSGGRLALQSERGAKRVKAVVAYGDAGHTAG
                     YNAAAVDDNPGAGVNFDTVNMRNQAMTHQVELVGTNSFTFALEFDVEDTGANSAPVII
                     FVPSGPYIVPLGSNAVFSVSVSDADGDPLLTTNVFAPSGLNVSGGTNFSWLAATNWFN
                     TTNAFVFIANDQKGKTNSVITNTTQIIVAYDWNANGMDDLWEWGNFTNLDAQADGDAD
                     GDRFDNYAEYIAGTQPNNPASLLSAAGTKAGANWRIAFGTVTGRTYTVWHAETPGDTW
                     TWAAEVSGTGSNVVWTDTGVSNLMRRIYRIEVEKD"
     assembly_gap    10591..10600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10601..11481)
                     /locus_tag="PKK36_03995"
     CDS             complement(<10601..11481)
                     /locus_tag="PKK36_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR93754.1"
                     /translation="MKKTLWAFMLVSFVMCAVSFGETMLQYFNTSWEEITARIPELAE
                     AGYTALWLPPPTKGSGGLSVGYDLWDPFDLGGNDQRGSVRTRYGTEAELLNLVETAHR
                     FGIRIYFDNIMNHRAFDIPGYNENTPIDIYPGMVPEDFHLRVTEDGFYRKWDNCRDWN
                     SAWQVMNLGLADLIDIAHETPNDNFGPNEGDDHPKISFVRQPNNPEYYYDTDLPISVG
                     YGEYTWNVYTFADKEPYTDTNGNGRFDWTDTNANGQHDSGEASEPFTDTGLDPTRPGW
                     NTTAYGYGDGKYNMGNPV"
     gene            complement(11630..12190)
                     /locus_tag="PKK36_04000"
     CDS             complement(11630..12190)
                     /locus_tag="PKK36_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNR93755.1"
                     /translation="MKKHLKWLLVFQLAGVYLAAAQEWTVMKNCTLEDNFSNDGDSFH
                     VKYQGRSLYFRLYFVDSAETNEEFPQRLNEQADYWKIDSKDALKVGKEAKHFTKNQLA
                     RPFTIYTQWKDAKGGSGKRYFAFVTTHSGEDLGELLAASGLVRVYGASTRHPDGTDAN
                     KMFDRLRKLERSARNHHLGAWSRQYR"
     gene            complement(12254..12646)
                     /locus_tag="PKK36_04005"
     CDS             complement(12254..12646)
                     /locus_tag="PKK36_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNR93756.1"
                     /translation="MDKIRKFLANDRFARLAGVELLDVGPGRAKASLTVGEKHLNGAG
                     MVHGAAIFVLADFTFAAASNAYGTLAVGINVSISFMKAVKSGVLTAEARETSRNAKLA
                     TYDIPVRDETGDLVAGFQGMVYRKKETI"
     gene            complement(12663..13982)
                     /locus_tag="PKK36_04010"
     CDS             complement(12663..13982)
                     /locus_tag="PKK36_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR93757.1"
                     /translation="MKSCNVFSALIEPIQRAVAEEGYTVPTPIQQQAIPHLVEGRDLL
                     GCAQTGTGKTAAFTLPLLQELTNSHKHPVSRRPRALILTPTRELAAQIGDSIRTYGRH
                     LKISHTVVFGGVGQNPQVQALRRGVDVVVATPGRLLDLMEQGHVKLDGIEIFILDEAD
                     RMLDMGFIPAVRRVIAALPRKRHSLFFSATMPPVVAELAKDLLHNPVEITIDPERPTV
                     ERIAQKMMFVDKGNKDALLVDIIAGHNMDKVLIFTRTKHGADKVVKKLRAADIAACAI
                     HGNKSQTDRTKALHGFKTDRVRALVATDIAARGLDVDSITHVVNYDLPEEPETYIHRI
                     GRTARAGADGDAISFCSARERDWLRGIEKLIRKKIPEEMNHAWHSETARHATGEAARP
                     EPRAQQGRLRAGGHARQQSPRDRRHPGGSGHKPHKFGKKSFGRLSGR"
     gene            complement(14136..15869)
                     /gene="argS"
                     /locus_tag="PKK36_04015"
     CDS             complement(14136..15869)
                     /gene="argS"
                     /locus_tag="PKK36_04015"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR93758.1"
                     /translation="MKNKRLSLQQRLTDWMTSRLRAVFPDMDFASVEMQVQPTANPQF
                     GDFQWNAAMKLAPRAGKKPRDVAGALVGSVPPPEGVARLEIAGPGFVNFYLDDASLAR
                     ELMEMEADERLGVPEPGRGHRVVMDYSSPNVAKPMHIGHIRSTVIGNALDRLHRFMGY
                     DVIADNHLGDWGTQFGIIIMGYRHFLDREALANAPVEELERVYRVSYERSKEDEEWLN
                     QARAELVKLQAGDPDNLALWEEFIRLSKQEFDRTYKRLGVSFDIERGESWYRDRLAGV
                     ISLLKERGLYEESEGAGVVKLDAEGLPLSIVRKSDGGYNYTTTDLATVISRVEEFNPD
                     RIVYVTDERQQLHFRQFFAIAAKLGFKVKLEHVWFGLMRLPDATFSTREGNVIKLERL
                     LDEAVSRAREMVRAASPDMPEENQELVAEAVGIGAVKYADLSQNPQSLVTFTWEKALS
                     LEGNSAPYLQYAYARIASVRDKYHEQFPGRNFLDYPIVLGEEVERRLAILLTRFGDVL
                     VTATNACRPNYLADYLYDLSQIYSAFYQNVPFLKAQDGVRESRIRLCDLVARTLRTGL
                     ALLGISSPDRI"
     gene            16108..16338
                     /locus_tag="PKK36_04020"
     CDS             16108..16338
                     /locus_tag="PKK36_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNR93759.1"
                     /translation="MKKAAVKKAAPKKKAAAAKAPAKKTEAPKARVISSEERYQMIQT
                     AAYYRAQANGFQGDPRIHWAEAEKEVNKLLGS"
     gene            16493..17269
                     /locus_tag="PKK36_04025"
     CDS             16493..17269
                     /locus_tag="PKK36_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93760.1"
                     /translation="MTKYFLLCLAVFSLVMSPVCFAESSVVETEVPAEDAESRNTLAE
                     AAKAASPGSDKQADEKTTKDLDKPVSRAARTEKEKSKTEPKIARAPRENRWDYVLGAG
                     IVISPDYKDMMEEAYGSVDASTIGWVDLQLGLRYKLSEYFTLTPGLDILFNYFVPEEE
                     DRFISHILLPSLGIQYAFENNPAYYLGAEISYPVADSGSDELDLSASGPGFSAFLGYM
                     MENEWNLEFGYSFLPVENGPDDEAEEYNLGGFVLKINRRF"
     gene            complement(17282..17962)
                     /locus_tag="PKK36_04030"
     CDS             complement(17282..17962)
                     /locus_tag="PKK36_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93761.1"
                     /translation="MNKRAVSAIFFLSLYSGSALSVKAAEAPGPAAVLTAVVQQMVTE
                     PVPEYLAQQVDWDSVLRATPARFAESQGIENGPALKELYLEFLRNPSKVLMKPLQPLI
                     SECLPQEKETVDRMVAAYESMNGWMVEELNEGLHSWKDAAYEIGEAGIDGDMATVKLR
                     VGDGRCFSDLSVNMVHVNGRWLLKGEWLPGLLNDIIEETGSRPESKTAGQAMNYLYRR
                     REEIRSGL"
     gene            complement(17979..19877)
                     /locus_tag="PKK36_04035"
     CDS             complement(17979..19877)
                     /locus_tag="PKK36_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNR93762.1"
                     /translation="MQVVNSAENVGRGGRRASRLLLLVLLGMAFIVNSFIAGLIYRDN
                     PLAADVSAAVGAILLALPIFWTAIRGLLAGSLQMNELVGLAVLAAFVFGDFQTAGLIS
                     FFMLISLVIETRTAEGAHASIESLVRLTPTKALRLLPGGGTEEVAAPDLKPGDIIRLQ
                     PGDNVPADGVIQKGQTTLNEATITGESVPRDKGPGDEVFAGTHNLTGSVEVEVARAGA
                     DTTLGQVRDLILAAGDTRLPFMRLIDRYTGYYTPVILMIAVLVWFFTQDASRLISILV
                     VACPCALILATPTAMVAALSAAARVGILVKHVADLEAVSRITAFVFDKTGTLTTGELG
                     VARLAPCEGVSPSDLLAAAASVDRFSHHPSAAAVLRLAEETGVEASDPEDFHEEPGRG
                     VRGNVRGGQVLCGRAGWLKENGIRSSKLDEADAGETEGFSVVFVARAGQYLGWIGMQD
                     EVRPGAKPCFEALRSLNVKRLAMVTGDRRPVAERVASAVGCGEFEAECFPQQKVSFVN
                     RLRDAGLSVAVVGDGVNDAPALAAGDIGVAMGAAGSDVAIHSATVALMNNDLNRIPFL
                     VGLSRQARAVVFQNLGIGLLFIVGGLMLSGMGYLNPVVAAVLHNTGSICVVFNSARLV
                     RTGGEFEA"
     gene            complement(19879..>20591)
                     /locus_tag="PKK36_04040"
     CDS             complement(19879..>20591)
                     /locus_tag="PKK36_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNR93763.1"
                     /translation="PPPPGPTLPPLRDGYTLSADANLLHSRWAVRYRISDPEQFLFAF
                     GAPEQILRSELDRAVVLASSRFGIDEAMRSEIESFRSAVDTCLRERCRELGLGVQVDR
                     VDLLAVIPPRQVAQAFDAVISAEQEQSRSISEARSYAARLDNEAQGEAARLKDEGEAA
                     RRRIVSEVRASADYFTTVREKYVKNPDITAQVLLQDVLRRVLTGVDEKYVIHRNADGT
                     QELRLLLTPEESKPVKKK"
BASE COUNT         4066 a         6230 c         6008 g         4277 t
ORIGIN      
        1 ctcgttcagc gaacggactt ttaatccgta attcacatga tgaaaaaaga atgttgaaac
       61 cgcactctcg aaggattcgt tcggatacgg aagattttca gccaaggcgg cagcataggc
      121 gatgtttgaa accttagcgg cctttctttg cgccacctca atcattttcg gggcggcgtc
      181 cacccccgtg attcggccac cgcgattcaa taccaaagca atttccatcg tcagtgtgcc
      241 cgtgccgcag cccacatcca gcaccgcttc agagccgccc agttccagca gccggatggc
      301 cgccttgcgg tactttcgct caagaccgaa ggtcatcagg ggagaaagca catcatagac
      361 agcggccgca tggtccagtg tgcggcccga agtctccagc gggtgcgcct catgcatact
      421 gcaaatcccc cagatccagc atcgccagca tcctgcggga catggactcg gcatgacgcc
      481 cggggatgcc aagcccctcc tctcccagcg ctgcaagcgc tccgctcaaa agcccgaggc
      541 cgcggcggac ggccggcaga accaccatcg gttgatcctg atacagccga tcccccgcct
      601 tcagcccggc agcggcaagc cgacgatacg gctccggaca atctcgagcc atgaccgaga
      661 ggcagtaatt gtgccggcgc tcgacataat cgcgcgccat gtcccgaaca tcgtcgagaa
      721 gctggcagcc caaaccgaaa tcgagcagcg cgcctttgac tcgctgcatt ttcgatgtgt
      781 ctatggagtc ctccacaacc tccgggccca gaaatggaat gtggaaaagc agtcccgtct
      841 tcagccggtg gatggtgcgc agcacatatt cggggtcagg gcggtcagtt accccgccct
      901 cctccgatgc ctcctgcgcc gcgctgggca ggagcacctg cagcgagcgc cgggaaagct
      961 cccggccctg atccccgctg atcacgccgg cctgaaccgc ctcgtcaaga atgcagttca
     1021 tcacccggtc ggcggccatc agggaaacca cctgcctcat gacatgcgac accccgggca
     1081 ggcgtacagg gagcacaatc ttgtcctcgt tatccagaag gttgtccgcg ctcgtaaccc
     1141 agatgcggaa cagatgaatc agctgaccat atagatgccg ccgggccggc gcaatatcca
     1201 gaagctgata cgcggactga aacagggtgg agaatatgtt ctttcgggcg ctgtagaagg
     1261 aaatcggaat ggattccagg ctcgtctgga aatcgggctc agtgcccgga ggtgtttcga
     1321 ccagcgcgcg cgcgtggtca tacaccgatg caatgcttcg gcgctgctct tccagcgcgg
     1381 ttctcaaaat gtgtccgccg ttccaaacct ttttcactca tgctccgttc tccccagcat
     1441 ccaagattca tcatgctttg cagattttca agtcggccgg acattttttc tgcaatcccc
     1501 gggagttcgc acacaaaaag ttccaatgat tggaagtttt tggcgaaaaa gttccaggca
     1561 ttggaagttt ttggcgcagg ttttccagtg attggaactt ttacaccagg tcgcaggcct
     1621 cgggcggcat ccagtgggcg tcccggccct cccatttcac attgcacccg atgggattgg
     1681 tgacaggcac actgaccggt tttcccgaag tcagttcagc cagagcccgc tcaagatcgt
     1741 taacggtcat ttttgatgtg tcgcgcgggt tgtccacgcc gcgaccggtg tagaccagcc
     1801 tgcgggcctt gtcgaacaca aagaaatgcg gggtgcgcag cgcgccatag gcgcgggcca
     1861 cgttctgtga ttcgtcatga agatagaccc acgggaactt ctgctcgttc atgcgggcca
     1921 ccatatgctc gaagtcatct tccgcatacg tgtttctgct gttggagttg atggcgacaa
     1981 agcggacgcc gcagtcccgg aatttcaagg ccgtcgcacg ggtcacctcg tccgacccca
     2041 ggacataggg acagtggttg caggtgaaga agatcaccag cccttcggca tctgaaaaac
     2101 cggcgagtga atatttattt ccgtccgtgg ccggcaggct gaaatccggc gcggtcattc
     2161 caacttcaag agtaaagctc attgcgcatt tccctttctt cagtagttgg cataaagata
     2221 cccgttattc tccatccagt ccgcgatttt gtccacagcc gccacaacat cattatcaga
     2281 aatatccaaa tccatcacgg gcaggcatga tttgaatacc agatcgcgca tcagtccctg
     2341 ctcgcggata aacatcttca gatcatcgta ctgatcaggt tttcccgaga ccttgagccg
     2401 ctcgcgccgg gcggcctcaa aagattccgg agaacggttc agaaacacga tcacgaaccc
     2461 cacggccttc agccgcttct ccagccactg gaaattcggc gccgctccgc gcgcctgatg
     2521 ctggaaggcc tgcgtggata tgtggaaacg gtctatgatc cacgaataat atttctgcag
     2581 ctcgagaagg cgcatccacg cttcatacgt ttccatcgcc ctctcttctt cctcgggctc
     2641 atagttgatc agccccctgc cccacggcca gtttgtgaac gcgcaccatt cggcggatat
     2701 cagcggcgag tgataacggt acctgcgcgg cccgacaatg cgcggatgct cgttgagtgt
     2761 aaaggccaca tcggttttga aggtcaggcg cgtgccttca agaattatct tggggcatag
     2821 tttcccgccc atcagtcttt ctcgacctcg atgcggtaga tgcggcgcat cagattggag
     2881 acgccggtgt cggtccagac cacattggat ccggttccgg aaacctccgc ggcccatgtc
     2941 caggtgtcgc ccggcgtttc cgcgtgccag acggtatagg tccgcccggt caccgttcca
     3001 aaagcaatcc gccagtttgc gcccgccttc gtgcccgcgg cggaaagaag gctggccggg
     3061 ttgttcggct gcgtgcccgc gatgtattca gcgtagttgt caaaccggtc tccgtcggca
     3121 tcgccgtcgg cctgcgcatc aaggttcgtg aaattgcccc attcccacag atcgtccatg
     3181 ccgttggcgt tccagtcgta tgccacaatg atctgcgtgg tgttggtgat cacgctgttg
     3241 gttttgccct tctgatcgtt tgcgatgaag acaaacgcgt tggtggtgtt gaaccagttg
     3301 gtcgcggcca gccagctgaa gttggtgccg ccggaaacat tcaggcccga aggtgcaaac
     3361 acattggtgg tcagcagcgg atcgccgtcg gcatccgaaa cggaaaccga gaacacggca
     3421 ttcgaaccca gcggaacaat atacggcccc gaagggacaa aaatgatgac cggggccgag
     3481 ttcgcacccg tgtcctcaac atcaaattcc agcgcgaagg tgaaactgtt tgtgcccacc
     3541 agttcgacct gatgagtcat ggcctggttg cgcatgttca ccgtgtcgaa attcacgccg
     3601 gcgccgggat tgtcatccac agccgccgca ttgtaaccgg cggtgtggcc ggcatcgccg
     3661 taggccacca cggccttcac acgctttgct ccgcgttcgg actgaagggc caggcgaccg
     3721 ccgctgacgg ccggatcact cagaatctgc tggccgtaca gcatcaggcc ttctgtgtcg
     3781 ggcgacagcc cgttgcgaac ataaaggacc cctccgttga gcgtttcgga aatggaatag
     3841 gccacttcca gccggttggt ctttggacca agggtcaggg tcttggtgat cttgccgtcg
     3901 gcggaggtga gccgccagcc gttggtccag ttgacggcgg cgtacatcat gttcacatag
     3961 ttggtcgtgc ccgcccacca gtcctttaaa catgaagtgc ggaaggcctc tacgcttcca
     4021 ttagtcgcga cattcgccgt gccttcaagt tctgtttccg aaccggaata cgccatcggg
     4081 gtgccggcca cagggaacac ggacccggcg ctgtcgcgag caaaggccat gatcatgcgg
     4141 ccgcctgtgc gctccatgat gaacatcacc cggccgttgt acagcagaca ctcggtttcg
     4201 ccatccaggt caacatcctc cagggccgct gtcgtgttcg tgaggccggc ggccgcagcg
     4261 gcccactggt ccacccgccc gtacaccgcg gccatgcgcg tctgcgactg ggcgttcttc
     4321 gcgaacccga tcagactgtt gtaggtggtg tcgggatata tgaaatcgcc cgtgctgaaa
     4381 cgccgcagat cattgttgtc ctcttcgtgg aacgcggttt caaaaacaga tgcgtggatg
     4441 gccgcgcgcg ccagcctcgc aaggctgtcg tcggaaacgg atttcacctt ctcccatgcg
     4501 ctggacacaa tgccgttgta ataaagcatg ccataggccg ccggaagatt tgttccgggg
     4561 cggatttcaa acaccttatt ctgaaggccc tcctcctgag cggagccgac ataccagttg
     4621 tcgtagttgt tctgcgtggc gtgctgcacc catatatgac ctgttttcgg cggggtggac
     4681 gcgccgcggt caaggtgccc ccagtagtcg ccgccgccgt cgccggtgat gtcaatccgg
     4741 cccgcggcga tgtcctccag cgccaccagt tgaatccacg gatggttggc cagccagcgg
     4801 atgttgcggt cataggcatc ggcctgcgcg ctgtttccga aatcctccca gttgctgagg
     4861 atcgtggcca cctggtcctg cgtgtcgctg cgggcgcggc ggttgagcat ttcccgcagc
     4921 ggaatgctga gcccgccgtc ggtgttgaca aaccggtagt cgccggcgct gttgttgatc
     4981 acaaaacagt agacgccgtt aatcttgttg atgcggtagc cgtcgtcgct caaagcgggt
     5041 gtgcggccaa tccagttcca catgtgcgtg ttctggtcaa tcagcgtgaa gcggtatccc
     5101 atgttggtga tttttccaag cacatcggca tccagcacgc gctcgggcgg ccaaaacacc
     5161 gtgctgtcac tgttcgtgaa gctgtagatg gtcttcagat ggtcgcgagc cagttcggca
     5221 ttgtcgttgt taaattccgg cgtgaaatac ggcaggatat gatccgaaaa tgtgctgccc
     5281 agaagcttga ccgtgttcgt caggatcatg gagcggatcc gttcattcag ggccggaccg
     5341 tccagccagg gcttgcccgc gtttgtgtcc gcgcgcgccc actggatggc cgaggccagc
     5401 gtgggggtga catgcaggtt caggggctga tcaaagacat cgtgaatttc cagcgcgcgg
     5461 tgataacccg cgccggatcc atcattaatc agattctgaa tttcgctgcc cggggtgatg
     5521 tgctgattgc catgcaccag catggccact tttgcggcct ttccgtaatc attcgcggca
     5581 ttccggccca cccagctgta cagttttccg ttcagctgga tgtttccctg atcacgccag
     5641 tagtcagagc agacccagtc atccgtaatg gaatcgcgca ggtcgttgcg gccgccgatg
     5701 tcgcccggcc cgacagggct gttctgcgtt ccgtccttca tcgtgaagac ctgatagttg
     5761 agctgaccgg gattcagtcc gttccagccc gcatccagca gcgcctggcg gctgatcgaa
     5821 aattcaaccg catcgagttc ggaattaaaa taggcctggc cgaatccgtt tgccgtgttc
     5881 tggtcgcggc cgaccacgcc atacggcgac aggtcatcca gcgccgacac ggaattattg
     5941 gcacgctgcg tgtccacata aacccggccg ttgttgccgc catacacggc gacggcggcc
     6001 tcccacttca tttcagtcac catgtcggcc gaatccggca ggctggcttc accgacgccc
     6061 gggctgttca tgtctatgat gacatacaga tcaagatagc cgtcctcggc catcggctgc
     6121 aggtcaagca ggtcaatgcg gaaatggaac aggccatcac cgccggaacc ggccgacccg
     6181 ccgtcatgcg cgtaaaacgc gacgatatcg cgtgatgtgt caaacccgtc ccatgcgcgg
     6241 taaatatcca cggcctggct tccaccgttg ttgtagtcat ccagcgcaat caaatcatcc
     6301 gccgaccagt cgctgaactc ctgatagttg gttgcaccat tgatcactcc gatggccggg
     6361 ccgcggccaa tggtaatgga tccttcgggc ggaggaacgc cggtatccgc ccagcgcgga
     6421 ttggtcccgt tgaccagttc tgtcgcattg tcaaagccgt ctccgtccgg atcgccggcg
     6481 gcgccgtttt cggcatcgcc atccgatccg gaggctgttc gcaggctgtc cgttccgttg
     6541 tccagcggat cgagattgta ctgcgactcc cagccatcgg gaagcccgtc gccgtcggtg
     6601 tcggccttca gcggatcggt ttcggtccac gcctcgccgg cattgtaaat tcggttggag
     6661 ttgctgtcgc ccgcgatccg tccgttgaaa tccctgtcct cgccgtatcc gtcactcagt
     6721 ccatcgccgt ctgtgtcgga gttgccggga tcggtctcgg tccaggtttc tccggtgtcc
     6781 atttcgccat tgggccacga acggccaagc gacggtggtg atgcgggatc aattatctca
     6841 ccgtcaccgt ccacccatcc gttgcggttg gcatcctcga taccgtcggg cagaccgtcc
     6901 ccgtcggtgt cgggatttgc cggatcagtc attgatccat agagctgctt cgcgcgatcg
     6961 ccgccctcac tctggctgtt cacgccggga accttgccgt aattgtcgag cgtgttgtag
     7021 aagggcggat cgtagtcgga gcagaagttc ttccatccgt cgccgttggt gtcggcattg
     7081 gtgtctgtgt tggaagaggg atttctccaa ccgcattcca ggccatcagg aagcccgtcc
     7141 ccgtcgctgt cgggactctg cgggagggat cgtccgtagg cgaagtgaac atgaacctcg
     7201 ccgttgtccc aggtctcggg attgggctcg ggatctgtct cgcgggcatt cggaagagga
     7261 acagacgttg cctcgttcac atcgtttagt ccgtcatcat cgtcatcaag atcattggtg
     7321 tttgaattga cccattcacg cttaacgacc gtcgcattgc gcagcgcgga attcgaaact
     7381 ccgccggcag tggaaactgt ggccagcaga ccgtactgtc cggccaggag attcgtccac
     7441 aggcactccc agagaattga actgcccgag ttggtgctgg acagggaact gacccctccg
     7501 ccctgcgcaa gatatccgtc gggggcataa gtgaacgaca gcgagaccga cgccgcgttg
     7561 gtcccggttt cgatgcgaac agtggtgcgg cgctgctcat tggagggatt ctgcacatcc
     7621 ggaatgatga tctggtgggg cctgccgtcc ggcccgtact cgggcggctc ggttatcgcg
     7681 atgtatggtg cctctgcggc cggagtcgcc ctgaccagac gattggcaac gaggtccggc
     7741 aggctgtcgc ggtcaaaggt gacttcaatg ccgtgcagcc agtcggtcac tccgttgtag
     7801 agattcccca gcggcatgga gagcgcatga tactgatccg tttcattcca gtgaatggaa
     7861 taggtgtcgc ggctttgcgc cacgccgccg tcagtctttc cggattcgct gctctggatt
     7921 ttaatcagga accggtcaat gaggtcattg gagctgagcc cgtcggccag ggcctttgtg
     7981 aaatacgcct tcatgacata gccttcgccg acatagtcgc cgttctgctg cggccacgca
     8041 atgaacatgc ggacatccgg cccccaggtg taaaccgtgc gctgaagggt ggtgaagtga
     8101 cccgtggcat ccgtgtgatt ggtaaacgcc ggacgcgcaa cagacgacag ctcgcgcagg
     8161 cggaccttaa tggtcgcgtt gctcccgccg gaagggatgt tgttgtagtt gaagcgccat
     8221 tccttcggat acaccgtgga catgtcggac ggcgttgcgg cataggcccg cacccaggtc
     8281 tcgccgatat tgctgttcca gactccgttt ccgttgatgt ccacaaaggc ttcgccggtg
     8341 tcataggacc cgctggcatt gccgtcggta tagggttcaa atccgctgcc gttgccgttg
     8401 gcgaccccgg tcaccacgtc atcgttgttg gtgtcccagt cggcaatatg gaaccagacc
     8461 tccgtcacgg tgggatcggt gcgaaccacc acgccgtact gctggccggc gactgaatcg
     8521 ccgttgttcg ccggatactg aatgaaaccg gtcgggcaca tggcgtcata ataaaaggtc
     8581 tgtttgaagg tgttgtaaag ggccgcgcga ttgtcgcgct ccagaaacgc gcgggcgctg
     8641 atcatgtgga agccctcaac aaggcccgtg cgcatcgtgc cgtagtcggc atgaacccga
     8701 tgagacactt tcgacgggct gaaattggtg acctgccagg ttcccatgcg cgtggttttg
     8761 agcgcgacgg cattggcgtc agcgggccag acgatattcc acggcgcata ggttccgccg
     8821 tcctgctggc ggtaaccccc gatcttgtag cggaaaacgg tgttggacga catggcgggc
     8881 accgtcgcct tccaccagtc cttgctgtcg ccggaatcag aatgatcgaa tgaaaactgg
     8941 atcgtcctgg tatttcccgc gcccatgcct ccggcgccct cgggccatgt cgcgccgtcg
     9001 atcgtgtaat agaggcagac atgattaatg ttgttcggat ctccggtctt cacccagatt
     9061 gtgagattgg atcctgcggc ctgtgcgggt gcgggcgaaa aatgcagcgc tccctgatcg
     9121 gtggtggccg atgggctgtg ccagacaaaa aacgcggtct cgctgttatt cccgtcgttc
     9181 acgccatcgc tgggatttat ggtcatgctt cccgagccga tcgtgcagtg ataagtttcc
     9241 gcgccggcgg agccgatctt gttgctggcc gaattgaccg ccgcgaattt ttccctgtgc
     9301 acgcggctca caaaagaagg ctgctcgtaa cccacaaaaa tgtcatccga gagcgccggc
     9361 gggttgtcgc gcggatctcc gccggcgtga ttcgcgccgt tgagatccat gccgccgtcg
     9421 agcttcagca ggacattggc cgctgaaccg tccacatgcg cgacaaagtt tacattggtc
     9481 ggcgatgtga tgcgcggcac ggtcatcgta taggcataat ccgtgctgtt ggtgtccggc
     9541 aggctgtagg gattgaaatt ggcatcgcca tcagggccgt ccttgcgggt ataggaaagc
     9601 gtgcccgcct gcgcaccgtt ctggtaaatc gtcaccgggc ttccgccgaa acaggtccac
     9661 attgaagaag gatctggatt cttccatgag aatgcgaaat atccgccggc ggggacgatg
     9721 acactgtcta aatcagatgc ccaggtgtag aacccgccgc cataggatga gtagttgtag
     9781 agatacgcat tatcccccat cggagtgtgg ctgtagctga tgttgttccg aatgtcgcgg
     9841 gcctggcctg aagaataatt atcgttcagc atgaagagca tgcagacctt gtcgccgtcg
     9901 gtgcttccgc cctgccgcca gtcgaggcgc tcataggcca catagtcggc atccgaccag
     9961 acgcccttct ggtagccgcg ggcgaactgc tcgtggatgt aaagcagatt tggaacgcgc
    10021 acatcgcccc actggccgag gaacgatgtg ttggcatggc gcgggaacgc acccccgctt
    10081 tcgccgagcg tctcggcatg atggttgccg tcggtgtaga gcagcccgag ccccgcacgc
    10141 gtgaaataaa acgcatgctg cagttcgcgg cgcgcggcgt agtcgttgtc gtggctctga
    10201 gcatgcatca cgccggtgtc ggggctgaag ccgcctgcgc cgggcgaatc gtagccgttc
    10261 agtccgttcc acatgcttcc gaggcggttg ttcagctcgt tgcggagcgg gttgtccacg
    10321 aggcgcatgc cgcgaccgag atagtctccg taaccgggcg gctcgccgag gtgttcaccg
    10381 aacatcatcg cgtcgtcgcg cggagcctcg gtgttgaaaa ccgtgtcgcg gtgattatcc
    10441 cagtcgctga atccgcgggt gatgttgaac tggacctggg cgcctccgca gtaccccgca
    10501 tcgctggtgt cgcttccgta ttcgccgaag aaataggccg gcacatgttt cacggcatcg
    10561 aggcggaggc cgtcggcttt ggtgcggtcc nnnnnnnnnn accggattgc ccatattgta
    10621 tttgccgtct ccatacccgt aggccgtcgt gttccagccg ggtcgggtcg gatcaagccc
    10681 ggtgtcggtg aagggctcgc tggcttcgcc ggagtcgtgc tggccattgg cgttggtgtc
    10741 ggtccagtca aaacggccgt tgccgttggt gtccgtatag ggctctttat cggcaaaggt
    10801 gtaaacattc caagtatatt caccatagcc cacgctgatc ggcagatccg tgtcataata
    10861 atactcgggg ttgttcggct ggcggacaaa gctgattttt ggatggtcgt cgccctcgtt
    10921 cggcccgaag ttgtcgttgg gcgtttcatg agcaatatca atcaggtcgg cgagtccaag
    10981 gttcatgacc tgccaggcgc tgttccagtc gcggcagttg tcccatttgc ggtagaagcc
    11041 gtcttcagtc acgcgcaggt ggaaatcctc aggcaccatg ccgggataga tatcaatggg
    11101 ggtattctca ttgtagcctg gaatatcaaa tgcccgatgg ttcatgatgt tgtcgaaata
    11161 gatgcggatg ccgaagcggt gcgcggtctc gacgaggttc aaaagttcgg cttccgtgcc
    11221 gtagcgcgtg cggacacttc cgcgctggtc gttgccgcca aggtcaaacg gatcccacag
    11281 gtcataaccg acagagaggc cgccgctgcc ttttgtgggc ggcggaagcc agagggcggt
    11341 atatccggct tcggccagct ccggaatgcg ggcggtgatt tcctcccagc ttgtgttgaa
    11401 atactgcaac atggtttcgc cgaaagacac ggcgcacatc acaaaagaaa ccagcatgaa
    11461 ggcccaaaga gtttttttca tcacaccacc ctgaccaggc acaattcgac caccgcatca
    11521 cccatgtaaa acgttttacc cggaggcgcg gccgttgtac agcttcattg tcataaaata
    11581 ctctccgggc tgcgggcctt caagctggaa ggcccgaatt tcgggctttt cagcggtact
    11641 gccggctcca ggcgccgaga tgatgattgc gagccgagcg ctccagcttg cgcagacggt
    11701 caaacatctt gttggcatcc gttccgtccg gatgcctggt cgatgccccg taaacccgca
    11761 ccagcccgct ggcggcgagc agttcgccca gatcttcccc gctgtgcgtg gtgacaaaag
    11821 caaaatatcg cttcccggat ccgcccttgg catctttcca ttgggtatat atcgtgaagg
    11881 gacgcgcaag ctggtttttc gtgaaatgct tggcttcttt tcccacctta agggcatctt
    11941 ttgaatcaat cttccagtag tccgcctgtt cattcagacg ctgcggaaac tcttcgttgg
    12001 tttcggcgct gtccacgaaa taaaggcgaa aataaagtga acggccctga tatttgacat
    12061 gaaaactgtc cccgtcgttg ctgaaattgt cttccagcgt gcagtttttc atgacggtcc
    12121 actcctgtgc tgccgccaga taaactccgg ccagctgaaa aaccagaagc catttgagat
    12181 gtttcttcat gtcacgctcc ctgcagattg aatcactgca gccatattac agaatctgcg
    12241 cacctgttgt cactcagatc gtttcttttt tgcgatacac catcccctga aaaccggcga
    12301 ccagatcccc ggtttcgtcc cgcacgggaa tatcataggt cgcgagttta gcgttgcgag
    12361 aggtctcccg ggcctcggcc gtcagcacac cgctcttcac cgccttcatg aatgatatgc
    12421 tgacgttgat acccacggcc agcgtcccgt aagcattaga cgccgccgca aaagtgaaat
    12481 ccgcgaggac gaaaatggcc gcgccgtgga ccatccccgc tccgttcaga tgtttctcgc
    12541 caacggtcag ggatgctttc gcccgaccgg gtccaacgtc cagcagttca acccccgcaa
    12601 gccgcgcgaa acgatcgtta gccaggaatt ttcgtatttt gtccatgcaa tctgctgaaa
    12661 tatcatcttc cagacaggcg gccaaaactt ttcttcccga atttatgcgg tttgtggccg
    12721 gatccgcccg gatgccggcg gtcgcgcgga gactgctgcc gagcatgccc gccggcgcgg
    12781 agccgaccct gctgggcgcg cggttcaggc ctcgcggcct ctccggtcgc atgacgggcc
    12841 gtctcggaat gccatgcgtg attcatttct tccgggattt tcttgcggat cagcttctct
    12901 atgccgcgca gccagtcgcg ctcgcgggcg gagcagaacg agatggcgtc gccgtccgca
    12961 cccgcccggg cggtgcggcc aatgcggtga atgtaggttt caggctcctc gggcagatca
    13021 tagttcacca catgcgtgat gctgtccacg tccagcccgc gggcggcgat gtccgtcgcc
    13081 accagcgcgc gcacccggtc cgtcttgaat ccgtgcagcg ccttcgtgcg gtccgtctgc
    13141 gacttgttgc cgtgaatggc gcaggcggca atatccgccg cgcggagctt cttgaccacc
    13201 ttgtccgcgc cgtgctttgt acgggtgaag atgagaacct tgtccatgtt gtggccggcg
    13261 ataatatcca ccagaagagc atccttgtta cccttgtcca caaacatcat cttctgcgcg
    13321 atgcgctcaa ccgtcggacg ctcggggtca atcgtgattt ccaccggatt atgcagcaaa
    13381 tcctttgcaa gctccgcaac caccggcggc atcgtggcgg agaaaaagag cgaatgtctt
    13441 ttcctgggca gggcggcaat cacccggcgc acggccggaa tgaagcccat atccagcatg
    13501 cggtcggcct catcgaggat gaatatctca atgccgtcga gcttgacgtg cccctgctcc
    13561 atcaggtcca gcaggcggcc gggcgtggcc accaccacat ccaccccgcg gcgcagcgcc
    13621 tgaacctgcg ggttctggcc gaccccgccg aacaccaccg tgtgcgaaat cttcagatgg
    13681 cgcccataag tcctgatgct gtcgccgatc tgggcggcca gttcgcgcgt cggggtgagg
    13741 atcagcgcgc gcgggcgcct gctgactgga tgcttgtggc tgttcgtgag ctcctgcaga
    13801 agcggaagcg tgaaggcggc cgtcttgccg gtgcccgtct gcgcgcatcc gagcaggtca
    13861 cggccctcca cgagatgcgg gatggcctgc tgctggatgg gggtgggcac ggtgtaaccc
    13921 tcctcggcca ccgcgcgctg aatcggctcg atcagagccg agaatacatt gcatgatttc
    13981 atttctcttc ttttccgtcc ggtaattttt caaaccattt ctgcctgccg gacgtggagg
    14041 aaaagaaaat gccgcgcgaa cgcggacgcg ggttcttcct gaaccaatgt gcgtgcggaa
    14101 gatacgcgga cggcggcccg gtgtcaaaca cagcttcaga ttctgtcagg agaggagatt
    14161 cccagcagag cgaggcccgt gcgcagggtg cgcgccacga gatcgcagag acggatgcgg
    14221 ctttcgcgaa caccgtcctg cgccttaagg aatggaacat tctgataaaa cgcgctgtag
    14281 atctgtgaca ggtcataaag ataatccgca aggtagttcg ggcggcaggc attggtcgca
    14341 gtgaccagca catcgccgaa ccttgtgagc aggatggcca gccggcgctc aacctcttcg
    14401 ccaagaacga tcggataatc caggaaattc cggccgggaa actgctcatg gtatttgtcg
    14461 cgcacgctgg cgatgcgggc ataggcgtac tgcagatacg gcgcggagtt gccttcaagc
    14521 gacagggcct tttcccatgt gaaggttaca aggctctgcg gattctggct caggtcggca
    14581 tatttcacgg ccccgatgcc cacggcctcc gcaaccagct cctgattctc ttccggcata
    14641 tcgggactcg ccgcgcgcac catttcgcgc gcgcgcgaca ccgcctcgtc cagaagccgc
    14701 tccagcttga tcacattgcc ctcgcgcgtc gagaaggtgg catcgggcag gcgcatcagg
    14761 ccgaaccaca catgctcaag cttcaccttg aagccaagtt tcgcggcgat cgcaaaaaac
    14821 tgccggaagt ggagctgctg ccgctcgtcg gtgacataga caatgcggtc gggattgaac
    14881 tcctctacac gggagatcac ggtggcaagg tcggtggtgg tgtagttgta accgccgtcg
    14941 cttttccgga caatgctgag cggaaggcct tcggcgtcca gcttcacgac tcccgcgccc
    15001 tcgctctcct cgtagaggcc cctctccttc aaaagcgaaa tcacgccggc aagacggtcg
    15061 cggtaccagc tttcaccgcg ttcgatgtca aacgaaacgc cgagccgttt gtaagtccgg
    15121 tcgaactcct gtttgctgag gcggatgaac tcctcccaga gagcaaggtt gtccggatcg
    15181 ccggcctgca gcttgacgag ttcagctcgg gcctggttca gccattcctc gtcctctttg
    15241 gaccgctcat agctgacacg gtatacccgc tccagctcct caaccggagc attcgccagc
    15301 gcctctctgt cgagaaaatg ccggtagccc atgataatga tgccgaactg agtgccccag
    15361 tcgcccagat ggttgtcggc gataacgtca tagcccatga agcggtgcag gcggtcgagc
    15421 gcattgccga tcaccgtgga gcggatatgg ccgatgtgca tcggcttggc cacattgggg
    15481 ctcgaataat ccatcaccac gcgatgtccc cggccgggtt caggcacgcc caggcgttca
    15541 tccgcctcca tctccatcag ctcccgggcg agtgacgcat catcaagata aaaattcacg
    15601 aagccggggc cggcaatctc aaggcgcgca acaccctcgg gcgggggaac agaaccaacc
    15661 agcgccccgg cgacatccct gggtttcttc ccggcccgtg gcgcgagttt catggcggca
    15721 ttccactgga aatcaccaaa ctgagggttc gccgtcggct gaacctgcat ctcgacggat
    15781 gcgaaatcca tgtcgggaaa gaccgcccga agacggctgg tcatccagtc ggtcagccgc
    15841 tgctgcaggg ataagcgctt gtttttcatc acgttatgtt gtccggataa atcatcagaa
    15901 ttcaggagcg ggattttaca caagaggccc caaaagggaa caaataaaaa taattgattt
    15961 cacccttgca ttggcagggg ggagttgtat tctcgccgcg gtcaaacggt tttacacgtt
    16021 gattcgaggt aattacgtta aattgaacag aaggggtgca gtatgccaat catgaagaaa
    16081 aaaaccggtg cgcggaaagc gcccgcagtg aaaaaagcag cagtgaaaaa agcggcgccg
    16141 aaaaagaaag cggcggccgc gaaagctccg gcgaagaaaa ccgaggcccc caaggcccgc
    16201 gttatttcgt ctgaggaacg gtaccagatg atccagaccg ccgcctatta ccgggcccag
    16261 gccaacggtt tccagggcga cccccggatc cactgggcgg aagcagaaaa ggaagtcaat
    16321 aaactgctgg gaagctgatc gcaggccgct ttcgccgggc agccccgtta acggggctgc
    16381 ccttttttat gagcattccc cgacaaagag acttgttttt cagaagccgt tccgttatct
    16441 actgcgcatt gaatctatgt gcgtgaaagg tctgtttaaa ggagatgcgc tgatgacgaa
    16501 atactttctg ctctgcctcg ctgttttttc actcgtgatg tcaccggttt gttttgccga
    16561 atcatccgtc gtcgaaacgg aagttcccgc tgaagacgcc gagagccgaa acacccttgc
    16621 cgaagccgcg aaggctgcat ccccgggttc ggataaacag gccgacgaaa aaaccacaaa
    16681 ggatctggac aaaccggtga gccgcgccgc acggaccgaa aaagaaaaga gcaaaaccga
    16741 gccgaagatc gcgagggcgc cgcgcgaaaa ccgctgggat tacgtgctgg gggccggcat
    16801 tgtgatcagt cccgactaca aggatatgat ggaggaagcc tacggatcgg tggatgcctc
    16861 caccatcggc tgggtggatc tgcagcttgg cctgcgttat aaactttcag aatatttcac
    16921 cctcacgccc ggccttgata tcctcttcaa ctactttgtt cccgaggagg aagaccgttt
    16981 catcagccac atcctgctgc cctctctcgg catccagtat gcatttgaga acaaccccgc
    17041 ctactatctc ggcgcggaaa tcagctatcc ggtggcggac tcaggcagcg acgagctgga
    17101 tctttcggcc tccgggcccg gcttcagcgc ctttctcgga tacatgatgg aaaacgaatg
    17161 gaatctggag ttcggctaca gctttcttcc ggttgaaaac ggaccggacg acgaggcgga
    17221 ggaatacaac ctcggcggct tcgttctgaa aatcaaccgc cggttctgat tccgtccgga
    17281 gtcacaaacc ggaccggatt tcctcgcgac ggcggtaaag atagttcatg gcctgacctg
    17341 ctgttttgct ttcggggcgg gagccggttt cctcgatgat gtcgttcagc aggccgggaa
    17401 gccattcccc tttgagaagc cagcggccgt tgacgtgaac catgttgacc gaaagatcgg
    17461 aaaaacagcg accgtcgccc acgcgcagtt ttacggtggc catatcgccg tcaatcccgg
    17521 cttcgccaat ctcataggcc gcatctttcc atgaatggag gccctcgttc agttcttcga
    17581 ccatccaccc gttcatgctt tcataggcgg ccaccatccg gtcaacggtt tccttctcct
    17641 gcggcaggca ttccgaaatc agcggctgca gcggcttcat cagcactttt gaaggattgc
    17701 gcaggaattc cagatataat tccttcaagg ccggtccgtt ttcaatcccc tgtgactcgg
    17761 cgaaccgcgc gggcgtggcc cgcagcaccg aatcccagtc cacctgctgc gcaagatatt
    17821 caggaaccgg ttctgtcacc atctgctgca ccactgcggt cagaacagcg gcggggccgg
    17881 gagcttccgc ggccttcacg gacagcgcgg aaccgctgta cagcgacagg aaaaatatag
    17941 cggacactgc acgcttgttc atctgcaaac tccttgtctc aagcctcgaa ttccccgccg
    18001 gtgcggacca gccgggcgct gttgaaaaca acacagatcg agcccgtgtt gtgaagaaca
    18061 gcggcaacca ccggattcag atagcccatg ccggaaagca tcagcccccc cacgataaac
    18121 agcagcccga tgccgagatt ctgaaacacc accgcgcgag cctggcgcga aaggcccaca
    18181 agaaacggga tgcggttcag gtcgttgttc ataagcgcaa ccgtggcgct gtgaatggcg
    18241 acatcactcc cggcagcgcc catcgccaca ccgatgtcgc cggcggccag tgcgggcgca
    18301 tcgttcaccc cgtcacccac cacggccacg ctcaaccccg catcgcgcag gcggttcacg
    18361 aatgacacct tttgctgcgg gaagcattcg gcctcgaatt ctccgcagcc gacggccgaa
    18421 gccacgcgct ccgcaacggg acggcggtcg ccggtcacca tcgccagccg tttcacattc
    18481 aggctccgga gcgcctcgaa gcagggtttg gcgcccggcc ggacttcgtc ctgcatgcca
    18541 atccagccga gatactggcc ggcgcgcgca acaaacacca cgctgaagcc ctcggtctcg
    18601 ccggcatcgg cctcgtcaag ctttgagctg cggatgccgt tctccttcag ccagccggcg
    18661 cgtccgcaca gaacctgccc gccccgcaca ttcccccgca cgccgcggcc gggctcctcg
    18721 tgaaaatcct caggatccga agcctccact ccggtttctt ccgccagccg cagaacggcg
    18781 gcggcggacg ggtgatgact gaagcggtcc acactggcgg ccgcagccag cagatccgaa
    18841 ggggaaaccc cttcgcaggg cgccagacgc gcaaccccca gctcgccggt ggtcagtgtg
    18901 ccggttttat caaacacaaa ggcggtgatg cgcgaaacgg cctcgaggtc ggcgacatgc
    18961 ttgacaagga tcccgacacg ggcggccgcg gaaagagccg cgaccatggc cgtgggcgtc
    19021 gcaagaatca gcgcgcaggg acaggccacc accagaattg aaatcagtct tgaagcatcc
    19081 tgcgtgaaga accagaccag gaccgcaatc atcaggatca cgggtgtgta gtatccggta
    19141 tagcggtcaa tcaggcgcat gaatggcagg cgcgtgtcgc ccgcggcgag aatgagatcc
    19201 cggacctgtc ccagcgtggt gtcggcgccc gcgcgcgcga cctcgacctc caccgaaccg
    19261 gtgagattat gcgtccccgc gaagacttca tctcccgggc ccttgtcgcg ggggaccgat
    19321 tcgccggtga tggtggcctc attcagggtg gtttgacctt tctgtatgac cccgtccgcg
    19381 ggaacgttgt cgccgggctg caggcggatg atgtcgcccg gcttgaggtc cggggcggcc
    19441 acctcttccg ttcctccgcc ggggagcagg cggagcgcct ttgtcggcgt caggcgcaca
    19501 aggctctcaa tggaggcgtg cgcgccttcg gcggtgcgcg tctcgatcac gagcgagatc
    19561 agcatgaaaa aggaaatcag gccggcggtc tggaaatccc caaacacgaa tgcggcgagc
    19621 acggcgagcc caaccagttc attcatctgc aggctgcccg ccagcagccc ccttatggcg
    19681 gtccagaaaa tcggcagcgc gagcaggatg gctccgacag ccgcgctgac atcggccgcc
    19741 agcggattgt ccctgtagat cagccccgcg atgaacgagt tgacaatgaa ggccatgccc
    19801 agcagcacaa gcagcagcag gcgcgatgcc ctgcgaccgc cccgccctac attttccgct
    19861 gaattgacca cctgcatttt atttcttctt cacgggtttt gactcctccg gcgtgagcag
    19921 gagccggagc tcctgcgtgc cgtcggcgtt tctgtgaatc acgtatttct cgtcgacgcc
    19981 ggtcaggaca cggcgcagca catcctgcag aagcacctgg gccgtgatat cgggattttt
    20041 gacgtacttc tcgcgcaccg tggtgaaata gtccgctgac gccctgacct cgctgacgat
    20101 gcggcggcgg gcggcctcgc cctcgtcttt caggcgggcg gcttcgccct gcgcctcgtt
    20161 gtcgaggcgg gcggcatagc tccgcgcctc actgatgctg cggctctgct cctgttcggc
    20221 ggagatcacg gcatcaaaag cctgcgccac ctggcgcgga gggatcacgg ccagcaggtc
    20281 cacccggtcc acctgcactc cgagtccaag ttcccggcag cgctcacgca ggcaggtgtc
    20341 cacggcggag cgaaagcttt cgatttctga ccgcatggcc tcgtctatcc caaaccggct
    20401 ggacgccaga accaccgcgc ggtccagttc gctccgcagg atctgttccg gcgcgccgaa
    20461 ggcaaaaaga aactgctccg ggtcgctgat gcggtagcgc acggcccagc ggctgtgcag
    20521 caggttggcg tcagcgctga gcgtgtagcc gtcgcgcagc ggcggcaggg tgggtccggg
    20581 cggaggggga t
//
LOCUS       DAOQWR010000031        20078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3043_length_20078_cov_5.808071, whole genome shotgun sequence.
ACCESSION   DAOQWR010000031 DAOQWR010000000
VERSION     DAOQWR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20078
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_20078_cov_5.808071"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..796
                     /locus_tag="PKK36_04045"
     CDS             221..796
                     /locus_tag="PKK36_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNR93764.1"
                     /translation="MMYNAVDLAALILVLLGTLQGLRRGLSGELASALSVVVAFFAGW
                     KFYAPVGRLIAEHTNMSGMLSNFFAFALCLVAGLLVMYSIGLLLHTLLVFTFRGKIER
                     IGGALCGTIRLLLITCAGIFLMGLLPSAFLQRHFAEESAVGRLLFAHLEPVRETITEK
                     IPVLKPEPEAMPEDEDYPEDEEIPFEELPVE"
     gene            800..2623
                     /gene="dnaG"
                     /locus_tag="PKK36_04050"
     CDS             800..2623
                     /gene="dnaG"
                     /locus_tag="PKK36_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNR93765.1"
                     /translation="MGQIISKQTIEDIRQRSDIVDVIGARVPLKRSGTTFKGLCPFHS
                     EKTPSFHVNPQRQIFHCFGCGAGGDVFSFLMKSEGVTFSTAVRMLAEKAGIPIELSEG
                     EDPDRDEKEVLLRIHEDAAAAYHQLLLKDAEAETARAYLARRDFSAETIASFQFGYAP
                     ERRGVAALLKGGKYTLEQLEKAGLMARSGEQAGQPGRPYDRFRRRLMIPIRDELGRVI
                     AFTGRVLDKTQKGGKYVNSPETRIFHKSRVLFNLDRARKAILDARMAIICEGQIDVIR
                     CVQAGIGHVVASQGTAFTEDHARILRRYTDTAVIAFDSDKAGQDAALKTAQLFLRGEL
                     VTQIAALPDGEDPDSLIQKQGPDAFLKQIGSALSLLAFQHRMMTRREDASKEVGRMRI
                     SRALLETVNMATSAMQRESMIREAAKLLNVPEAAVRNDLKQRRPAVQGPETPAAPKAA
                     QYPREELELLELVLAHPEAGKLLQVYLPPAHLQSPVCRRLLEHVLARPAGGEWNLMDE
                     LAGDEESCRVAASLQFNLRTLEQPDMTAETAAKALILVITRKKLERIREALRRKAAEN
                     PSPALEKRRMEITHDISLLRRDWEHAEPVLEVYADADEPSA"
     gene            2799..4733
                     /gene="rpoD"
                     /locus_tag="PKK36_04055"
     CDS             2799..4733
                     /gene="rpoD"
                     /locus_tag="PKK36_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNR93766.1"
                     /translation="MKAKPAGKAVKKPAPKPVSAKPKKTGKAAKKTAPKPVSAKLKKA
                     GKAAKKTAPVKPAEKAGKKPEAKQKKTAPAGGKKNAAQAGARPRVPAEESADENALES
                     RLSREQRNEMLKELISLSKEQRYLTYEQINDAMPDSMLNPDEVEGIVILLRGMDIEII
                     EETDVDTYKKQLEKDERARAAKRVDVLDDPVRMYLKQMGLVPLLTREQEVEISKRIEE
                     AEIHTKSLFHDFGCALSAYMAVIERLETGKERFDRIINDKFVENREKYFKALPRLRQN
                     LFRNQDALQRKFEALQRTRLTKSQRARIQKDFDKSRARMSELLDKLYFKQKAVEDIVS
                     STDEVHRDLRALMKELQSIEKNRKSKTREADLKNAQKKLRVLEISQRMNREDFLAKYA
                     DLKMWLRKGLVAKTEMVEANLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEY
                     RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRMQKRLVQELGREPT
                     PEEVSEEMDIPIERVRVVMKSAQQPISLQSPVGDSEDTNFGDFIEDKSADNPSEMASY
                     SLLKECIGEVLGTLTDREQEVLNLRFGLADGYPRTLEEVGRKFNVTRERIRQIEAKAL
                     RKMRHPTRIKQLQGFLEANA"
     gene            4807..6150
                     /locus_tag="PKK36_04060"
     CDS             4807..6150
                     /locus_tag="PKK36_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNR93767.1"
                     /translation="MTACPIQNIIKRDGNMVAYDRDRIATAIFKAATSVGGSDRTEAE
                     RMAVEVEKALVKNFGGKSTPSVEEIQDTVESTLIRMGHAKTARAYIIYRHERAAKRAQ
                     REKNIEASDNIPYKKIYEILRWNMDHHCETIDDLNRLIANGKFPDLIAASDERYSKEV
                     EAGAQEIIARLPEVRIAIIAGPSSSSKTTTTIKVSEKLEEAGVKFVAINVDHYFFDLD
                     HHPKDEFGDYDYETPHALDLELINEHLVALLEGKTIKTPHYNFKTGTRELNVHEMSLK
                     KNEILLIDSLHGLFDGMTSSIPASKKFRLYIETLGQIRNEQGIFMRWADNRLLRRMIR
                     DSWHRNLQPLQTLTHWHYVRRSEMKNIVPFIGTVDYLVNSAMPYELPILKARLFKFFP
                     EAMALYRNDAKRQDAYLRAKRVFEFLEPMTEVADDSCVPPKSLLREFIGGSAYKY"
     gene            6128..7705
                     /locus_tag="PKK36_04065"
     CDS             6128..7705
                     /locus_tag="PKK36_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNR93768.1"
                     /translation="MEAPTSIKNLSVPDALRQNAKLHAGAPAIVFGGRTLDFQTVDAQ
                     SDRVAAGLHARGIRKGDRVALYCINSDFFALAYFGIQKTGATVVPINLLLNPKEINYI
                     LKDSGAKAMIYFEAFAGNVKAATEDVELLGRACIGANKAGEGDLLWPELLSDQKPPAV
                     VFNPAEDVAAILYTAGTTGHPKGAMLTHTNLVSNAWSVSQALPLEPGKDSILLVLPMF
                     HAFAATAGMLYSMLHGICVIPLPRFEPHETAKALAATKAGMFMGVPSMYNVLLHLPDE
                     CTEMFSSLKYCISGGAAMPVEVMKAFETRYGKLIYEGDGPTECSPVTCVNPIGGKRKP
                     ASVGLPVPLVEMKIMDDTGKEVSHGTIGEICVRGPNVMKGYWNQPEETAASFFGEWFR
                     TGDLGTEDDDGYFFIVDRKKDMLIVNGMNVYPRVVEEVLYQFEPVREAAVIGEPHELH
                     GEIPVAYVALKEGRKATANDVRNYCRSNLGRYEVPRKVFFMDALPKNAAGKIMKRELR
                     THGELERGIDSRRDEPK"
     gene            complement(7708..8733)
                     /locus_tag="PKK36_04070"
     CDS             complement(7708..8733)
                     /locus_tag="PKK36_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR93769.1"
                     /translation="MITLEQYAAPEWAAGLSSAPDRVMRLAMRPTPLEKWKLPAADIW
                     IKRDDLTGCALSGNKVRKLEFLLAEASSAGCDTVITCGGIQSNHCRATAAAAVAAGMI
                     PHLFLSTDCPSADPGLEGNLLLDRLVGAKIHLITPEEYLRRDDVMRELAETLRSSGRR
                     PYIIPEGGSNALGAWGYLDAWEEMRLQMERAHLEITDAVVACGSGGTAAGMALGAALS
                     GAAVKVHAVNVNRDARYFYDRIGKILRDMGASQKAEDILDIMDGHVGLGYAVSTDEEL
                     RQIAGIASSTGIILDPVYTGKAFCAMVKELSKNPFRFMGRSILFVHTGGMLGLYEKLP
                     QLQSILR"
     gene            8962..14757
                     /locus_tag="PKK36_04075"
     CDS             8962..14757
                     /locus_tag="PKK36_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR93770.1"
                     /translation="MKAPLRRICLILLSILLPRGGPAAEHSGNGWIIRKTAKETAAES
                     GSTIFYVISVERTGPDAGEVNIEDWRGAAGGTTAYLWASPEGDGGGGTADYLNWTRTM
                     NQGDKFTAIYEVEVDTGLSAGTIINNYIMADTVPYDPDMHISLDHYLVVGGGNKTRTA
                     NRGCGQSSEPVSTATGEFFIPPVTDMDLGGPLPLKFTRWYGSRLNDPGIDLIGSSLGK
                     GWMHSFESRAIHSGFFSRWLRVILPGGRIASLAEVYGGEGGWQLAYEQEDVAYQGRED
                     GVSFWLMDPESERLYRFDAPPYSWGSTNRLREIADRNGNALLLEYRADGRITNVADGL
                     GRNLSFTYTATGNLLAVSDGARTTGFGCDAQGALISVTNPLGYVTTCIYDPVNSHTNG
                     QGALMTGIQHPRGNVPYTQAYNSNGQVVSQTSACGGMSSFSYTTTVYGETTQIDDPQG
                     SLVHNHLNWSRATNLVDQAGNGFGISYQTERNLPTRIRDRCGQASSFQYDFHSCRLTN
                     VIHRDGATTTYSYVTTTQVFTNRETGTNTVEFDFQDLCAIRHPDGSTEEFHRDARGNV
                     TSYVNRAGAVWTTTFNERGQPLTASRPGGGVQVFTYNPDGTLASEADSDTGAIIYTYD
                     ALRRRIRETRPNGGQTGFALDGLDRIVTITNTAGGTTSFQYDPNGICTNTAEPAGYTR
                     MRQTDLMNRVTNCADSLGPLAAISYDSMGRPVIETDPAGTNHYHYDDRGWLTNLTRAG
                     RTWSWSYDPEGHVAQVVSPSGNCTVYQRDPMGRVTNTIDGLGNAQSSSHDRMGRRILF
                     TDRNGLVTHFSHEPGGQVAGISNALGATASLGYNADGLITTATDFDGRITTAGYTPMG
                     RLAAITNALGEVTGYAYDTAGRLIRTDFPDGTHESLAYDLAGRLISRTDRGTNVWTCG
                     YNARGDLVAVTNPAGGITLRDYLSDGTLKWIRDSDVTNIFFVGDGARRLVEVIVPGGA
                     GIHYEYNAHHEITAIVDANSNRTDYAYDSEGRVVSETDAHGHSLQYEYDPVGRVTSIV
                     DRTGARSCYEYDAAGRLTAAADPAGVRVQYGWDPGNRLTSATLGGQTWTRIYDTAGRL
                     TAHSTPLNRTTSLIRDAAGRLAGHVDPMGRTNLIARDSMGRITAVTDPAGRARVFSCD
                     SRGLLVAAAATGQAAARYERDALGNVIRVTDPNSNAWTFSYSSMGALLGMADPLGRSN
                     VLMRNARGQISGILYPDGAASTTHYDPAGNVTAAVYSTGLILPYAYDHQSRLIGADGI
                     ALQRDPEGRTTNTISTSDGRAFGAAYDGAGRLAAAYYDGALTVTYIYNTTNGLLESVS
                     DSSSGAAVWFSYDADRRLTGITRGNGQHAAFTLDDAGRITRIQDGTLTDVQYEYDAAG
                     RLTAEEGTYPLEAGANLDPETNSFTYDPAAQISSSGFVYDARGRLTNAPGRRFVWDAA
                     SRLIDADGIQIAYDGFHDVLQKTAAGTTIRYYHNRAVRPASIVAERDETAGTFVRYYV
                     WTPAGQLLYAIDTASGNKPRYYHFDRIGSTLAITDENGQVTDAYAYTPFGRIVRRQGA
                     STQPFTFVGRHGIRQQDADGTLYQMRARYYNAQTGGFLSRDPGWPELFDARKINPYAY
                     AGNDPVGRIDCNGRWWMGPDIDPLPDDADVTMLSNWQLTEIELRLEEMDRDDNINLPE
                     VRIRKDRIRAELHRRNPIPLIIPPKLQDQEHTSCEQQPLNTAVQPAGQMVETPGLTGE
                     IPPPPARYRKPLDQWTPFDLAHEIFSPENMQRAAVEQHIAEREARERAEKEAAERAEV
                     ERTAREALEQFMRRTQGVPPEVKKPQYTLPESVFKPTPRPFRDPLEFILDSLFGPQAI
                     LDFAAQQQLQQQVQSQAQTEASKPAAAPEKPRIPTRDLYQNLQKAQRRAAELMQARDF
                     EGARRELDDARRWEQEIHERYQREWFTMYRD"
     gene            14769..15593
                     /locus_tag="PKK36_04080"
     CDS             14769..15593
                     /locus_tag="PKK36_04080"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HNR93771.1"
                     /translation="MKNHKICMQKAGRLLLTGILTGMILSIPAYAARIDYAYDETGRL
                     TNAAYSGGAGIAYAYDANGNMLIRETSAGGAATYTLIYLAGAGGSIAGIATQEVAAGD
                     SGSAVTASPADTSVVFHAWSDGLFDNPRTDTNVSANLTVSTVFRSEGGANLDWYAGHG
                     FVPEGGELWSDLDTKIASGKGTTLLHEFIADTDPNNTGDIFRVLSISNGPPVVVQFQP
                     GSTGRVYTFQYAGDLAGDAWTNVPGTVPRPGLGGQDAMTDTNPPPFRSYRIEVQAP"
     gene            15682..17259
                     /locus_tag="PKK36_04085"
     CDS             15682..17259
                     /locus_tag="PKK36_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNR93772.1"
                     /translation="MSKKQKYIKLQKSRRTRGVIIAIVLFIILLPYAVCWWERHPAGF
                     SVRSKPCPVPADSVRLLIDSTSFDAEKGERIFDQQIFDAVLKMIRDAKQTVYVDFFLW
                     NYWTGSIPESHRGLAQELSESLIAKKKESPETHILVMTDPVNRVYGGDEQPFYEAMRQ
                     AGICIVFTALDKMPESNFFYAAPARFYGPLISRIPGIRQLLNIRLLKHPFEEGGQNIS
                     LLQFGRLLFFKANHRKVVITDGPGGWQLLVTSFNPANGSSGHSNIGLQAGGEIAREAL
                     ATELQCVKWSAENPGNVLEPEPGACALIIGVLSERLSEPPLSSKADEKMTAEWLTEEC
                     IARKAEALLDACGTGDEVRLAMFYLADRKLITAIREAADGGADVRIILDANRDAFGRV
                     KNGVPNRPVAAELMEHAVREGVRLQIRWADTHGEQFHPKVLSISNAEGKADLLCGSAN
                     WTRRNLRDLNLEANLHLKNAPEVSRAFGEWFDLCWDNADGRSRTVPYEVFAVSGFERS
                     RKALMGRFQEATGMSTF"
     gene            complement(17310..18977)
                     /locus_tag="PKK36_04090"
     CDS             complement(17310..18977)
                     /locus_tag="PKK36_04090"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93773.1"
                     /translation="MSELGMLLKKDWRQAANLRHELAEQSRFKLAVILFFALGMLGGL
                     YGLFWEGFTFLERLGGIGLMIIHRLLAFCFLALTFLLILSSSVSAYGTFFGDRDMGFL
                     KLMPVRIGTLIRYKFLHAAFYASWAFFFIIVPFVMAFAMQQKLSGFALLASVVFSLPF
                     VMICSSAGALLCLVFIRWMPPRRMVKYILMAVILAVGIAGWKTFVVNVRLGEDVNVIL
                     NRVIPGLKLASHPLLPGYWASEGILSISRGQWARGLMFGLLLFSSAGMGSVLLEAAGS
                     RIYEEGYQKLMRGSRRHHRMHRARAVMERTLVFLPRDMRSILVKDICIFLRDPAQWVQ
                     ALFFLGMLGLYFLNLRNLRYHTLPLAFRNLIAFLNVFSVSAVLTSLGARFVYPQLSFE
                     GHAFWVLGLSPLSMKRILLSKFAGALFSMVFVSLLLMGICVRMLNIEAYAGMVSMAVA
                     FAVSVALSGFSCGLGALFMDLKHSNPSAIVSSFGGTLNLVCSLVFIFMTMFPFAFVFQ
                     LYYRGYLSPEMFVHALMLGGAWLVGITLAAVLVPLWLGLRSLMKREY"
     gene            complement(18991..19725)
                     /locus_tag="PKK36_04095"
     CDS             complement(18991..19725)
                     /locus_tag="PKK36_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR93774.1"
                     /translation="MSEHPPMIVIEDLRKTFGDVHAVAGLDLRVPAGELFCFLGPNGA
                     GKTTTIKMLCGLLKPGSGRIVIRGMDLHEHAQEVKKITGYIPDQPYLYERLTAAEFYE
                     FTGDLYGIPRDEIRAKREKAFELFGLEDYQDSLIKNMSHGIRQRLCYASVFLHDPAVL
                     FIDEPFIGLDPYSIRLIKNLLREKCAAGATIFLTTHILALAEDMADRIGIIDNGRLVA
                     LGTLHELMRQTDMQGRLEDVFLKLTE"
     gene            complement(19718..>20078)
                     /locus_tag="PKK36_04100"
     CDS             complement(19718..>20078)
                     /locus_tag="PKK36_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR93775.1"
                     /translation="RLHKGRGRQIARRIGDGIEVLRVGAGDCNEHTYLFVALARAAGI
                     PARIHLGVVYLDGAFYYHAWPSVFVGRWVDMDPTFGQHEADASHIFLGEGESAAQVRL
                     LQVVGQVKADILEAIYE"
BASE COUNT         4520 a         6121 c         5913 g         3524 t
ORIGIN      
        1 atgaggctat tcagatgctg tttatccttt gaatattttt accggacagt agtgattttt
       61 ggagcatttt tggccgtttt ttgcggtttt ttggccgttt ttggccattt ttggccgttt
      121 tttgcggttt ttgggcactt tttaagcata cgctttattg acaagcataa ttgaatccat
      181 ccggcggatg ggttagtctg gatcggtttt cgggagtttt atgatgtata atgcagttga
      241 tcttgcggcg ctgattctgg ttttgctggg caccctgcag gggcttcgcc gggggctttc
      301 gggcgagctg gccagcgcgc tcagtgtggt ggtcgccttt ttcgcgggct ggaaattcta
      361 tgcgccggtg ggccgcctga ttgcggaaca cacgaacatg tccggcatgc tctccaattt
      421 ctttgcgttc gcgctctgcc ttgtcgcggg cctgctggtg atgtattcca tcggccttct
      481 gctgcacacc ctgctggtgt tcacgttcag gggcaagatc gagcgcatcg gcggcgcgct
      541 ctgcggaacg atccgcctgc tcctcatcac ctgcgccggc attttcctga tggggctgct
      601 gcccagcgcg ttcctgcagc ggcatttcgc cgaggagtcc gccgtgggcc gcctcctgtt
      661 cgcgcatctt gagccggtga gggaaacgat cacggaaaag atccccgttc tgaagccgga
      721 accggaggcg atgccggagg atgaagatta tccggaggac gaggaaattc cctttgaaga
      781 gcttccggtg gagtgacgga tggggcagat tatttccaag cagacgattg aggacatccg
      841 gcagagaagc gatattgtgg atgtgatcgg agcgcgcgtc ccgctgaagc gcagcggcac
      901 cacgttcaag gggctctgcc ccttccacag cgaaaaaacc ccctccttcc atgtgaatcc
      961 acagcggcag attttccact gcttcgggtg cggcgcgggc ggcgacgtgt tcagcttcct
     1021 gatgaagagc gagggcgtga ccttcagcac ggcggtccgc atgctggccg agaaggcggg
     1081 cattccgatt gagctgtccg aaggcgaaga tccggaccgc gatgaaaaag aagtcctcct
     1141 gcgcatccac gaagatgccg ccgccgcgta tcaccagctt ctgctgaagg acgcggaggc
     1201 cgagacggcg cgggcctatc tcgcgcggcg cgatttttcc gcggaaacca ttgcctcgtt
     1261 ccagttcggc tatgcgccgg agcgccgcgg agtcgccgcg ctgctgaagg gcggaaagta
     1321 caccctcgaa cagctcgaaa aggccgggct gatggcccgc agcggggaac aggccggaca
     1381 gccgggccgg ccctatgacc ggttccgccg gcggctgatg atcccgatcc gcgacgagct
     1441 tggacgcgtg atcgccttca ccgggcgcgt gctggacaag acgcagaagg gcgggaaata
     1501 cgtcaacagc cccgaaacac gcatctttca caagagccgt gtgctgttca accttgaccg
     1561 ggcccgcaag gccattctcg acgcgcgcat ggccatcatt tgcgaaggcc agattgatgt
     1621 gatccgctgc gtgcaggccg gcatcgggca tgttgtggcc tcgcagggca ccgccttcac
     1681 ggaggatcat gcgcgcattc tccggcgcta caccgacacg gcggtcattg ccttcgattc
     1741 cgacaaggcc gggcaggacg ccgcccttaa aaccgcgcag cttttcctgc ggggagagct
     1801 ggtcacgcag atcgccgcgc ttccggacgg ggaagatccg gacagcctga tccagaaaca
     1861 gggtccggac gcgttcctca agcagatcgg atcggccctt tccctgctgg cgttccagca
     1921 ccgcatgatg acccggcggg aagacgcctc caaagaagtc ggccggatgc ggatcagccg
     1981 cgcgctgctc gaaaccgtga acatggcgac cagcgccatg cagcgcgaaa gcatgatccg
     2041 cgaggcagcc aaactcctga atgttcctga agccgcagtc cgcaacgatc tgaaacagcg
     2101 gcggccggcg gtgcagggcc ccgaaacgcc ggccgcccca aaagccgcgc agtatccccg
     2161 cgaagagctg gaacttctgg aactggtgct ggcccatccc gaggccggca aactgctcca
     2221 ggtctacctc ccgcccgccc atctccagag ccccgtctgc cgcaggctgc tcgaacatgt
     2281 cctcgcccgg ccggccggcg gagaatggaa cctgatggat gaactggccg gcgacgagga
     2341 atcctgccgc gtggccgcaa gtctccagtt caacctgcgc acgctcgaac agccggacat
     2401 gacggcggaa accgccgcca aggcgctgat cctcgtgatc acccggaaaa aactggaaag
     2461 aatccgggag gcgctgcgcc ggaaagcggc cgaaaacccc tcgcccgcct tggaaaaacg
     2521 gcgcatggag atcacgcacg acatcagcct gctgcgccgg gactgggagc atgcggagcc
     2581 cgttctcgag gtctatgccg acgctgacga gccatccgct taacaaatca tttaacaact
     2641 tccggggaaa tgcatattta ttcgtgcttg attcttgacg atggggccgt tttttgtact
     2701 ctacatggcc ttttcggggt ataatggatg gattgtgtcc agcaacagca ggtctgcggg
     2761 gttatatggc agttaaggca aagaaaaaga aaacagcggt gaaagcaaaa ccggccggca
     2821 aggcagtgaa aaagccggcc ccgaaacccg tgtctgcgaa gccgaaaaag acgggcaaag
     2881 ccgcgaagaa aacggcaccg aaacccgtgt ctgcgaagct gaaaaaggca ggcaaagccg
     2941 cgaaaaaaac ggcaccggtg aaaccggcgg aaaaagccgg caagaagccg gaagccaaac
     3001 agaagaaaac cgcgcccgcc ggcgggaaaa agaacgccgc ccaggccggc gccaggcccc
     3061 gcgtaccggc cgaagaatcc gccgacgaaa acgcgctgga atcccgcctt tcgcgcgagc
     3121 agcgcaatga aatgctcaag gagctgatct ctctttccaa agagcagcgc tacctgacct
     3181 acgagcagat caatgacgcc atgccggaca gcatgctgaa ccccgacgag gtggagggca
     3241 ttgtgatcct gctgcggggg atggacatcg agatcatcga ggaaaccgat gtcgacacct
     3301 acaaaaagca gctggaaaag gacgagcggg cgcgcgccgc caagcgcgtg gatgtgctgg
     3361 atgatccggt gcgcatgtac ctcaagcaga tgggccttgt cccgctgctg acccgcgagc
     3421 aggaagtgga aatctcgaag cggatcgagg aggcggaaat tcacaccaag tccctctttc
     3481 acgatttcgg ctgcgcgctg tccgcctaca tggcggtgat cgaacggctg gaaaccggca
     3541 aggagcgctt cgaccgcatc atcaacgaca agttcgttga gaaccgcgag aaatatttca
     3601 aggccctgcc gcgcctgcgc cagaacctgt tccggaacca ggacgcgctc cagaggaaat
     3661 tcgaagccct gcagcggacc cggctgacca aaagccagcg cgcgcgcatc cagaaggatt
     3721 ttgacaagag ccgcgcccgc atgagcgaac tgctcgacaa actctatttc aagcagaagg
     3781 ccgtggagga tattgtctca tccaccgacg aggtgcaccg ggatcttcgc gccctgatga
     3841 aggaactcca gtccattgag aagaaccgca agagcaaaac gcgggaagcg gatctgaaga
     3901 atgcgcagaa aaagctgcgg gtgctggaaa tatcgcagcg gatgaaccgg gaggacttcc
     3961 tggcgaaata cgcggacctc aaaatgtggc tccgcaaggg gctggtggcc aagacggaaa
     4021 tggtggaagc caatctgcgc ctggtgatca gcatcgccaa gaagtacacc aaccgcgggc
     4081 tgtcgttcct cgacctgatc caggagggca acatgggcct gatgaaggcg gtcgagaaat
     4141 ttgaataccg ccggggctac aagttcagca cctatgccac ctggtggatc cggcaggcca
     4201 tcacgcgctc catcgcggat caggcccgca cgatccgcat cccggttcac atgatcgaaa
     4261 ccatcaacaa gctgatgcgc atgcagaagc gcctcgtgca ggaactcggc cgcgagccga
     4321 cccccgagga ggtcagcgag gaaatggaca ttccgatcga gcgcgtgcgc gtggtgatga
     4381 aatcagcaca gcagccgatc tcgctgcaga gcccggtggg cgacagcgag gacaccaatt
     4441 tcggggactt catcgaggat aaatccgcgg acaacccgtc ggagatggcc tcctacagtc
     4501 tgctgaagga gtgcatcggg gaggtgctcg gcacactgac cgaccgcgag caggaggtgc
     4561 tgaatctccg cttcggcctg gcggacggct atccgcgcac gctcgaggaa gtcggccgca
     4621 aattcaacgt gacccgcgaa cgcatccggc agatcgaagc caaggcgctg cgcaaaatgc
     4681 ggcatccgac ccgcatcaag cagcttcagg ggttcctgga ggccaacgca taagcagaaa
     4741 agcttgcaga atgcccggga ttcactagca ttacgcggca cgacaactaa tagaaggaga
     4801 caccccatga cagcatgtcc cattcaaaac atcattaagc gtgacggcaa catggtcgcc
     4861 tatgaccgcg accggatcgc caccgccatt ttcaaggccg cgacctctgt cggcggctca
     4921 gaccgcacgg aagccgagcg catggcggtc gaggtcgaaa aggccctggt gaaaaacttc
     4981 ggcggcaaaa gcacacccag cgtggaggag attcaggaca cggtggagtc aaccctcatc
     5041 agaatggggc atgccaaaac cgcacgggcc tatatcatct accgccacga gcgcgccgcg
     5101 aaacgcgccc agcgcgagaa aaacatcgag gcctccgaca acatccccta caagaaaatc
     5161 tacgagatcc tccgctggaa catggatcac cactgcgaga cgattgatga cctgaaccgc
     5221 ctgattgcga acgggaaatt cccggacctg atcgccgcct ccgacgaacg ctacagcaag
     5281 gaagtggaag ccggcgcgca ggagatcatc gcccgccttc cggaagtccg catcgccatc
     5341 atcgcggggc cctcctcgtc cagcaaaacc accacgacca tcaaggtcag cgagaagctg
     5401 gaagaagccg gcgtgaagtt cgtggccatc aatgtggatc actacttctt tgacctcgac
     5461 caccacccga aggatgaatt cggcgactac gattacgaaa cgccgcacgc gctggacctg
     5521 gaactgatca acgagcatct ggtcgccctg ctggaaggca agacgatcaa aactccgcac
     5581 tacaacttca agaccggcac ccgcgaactg aatgttcacg agatgagcct gaagaaaaac
     5641 gaaatcctgc tgattgacag ccttcacgga ctgttcgacg gcatgaccag cagcatcccg
     5701 gccagcaaga agttccggct ctacattgaa accctcggac agatccgcaa cgagcagggc
     5761 atcttcatgc gctgggccga caaccgcctg ctccgccgca tgatccgcga cagctggcac
     5821 cgcaacctgc agccgctgca gaccctgacg cactggcact atgtgcgccg cagcgaaatg
     5881 aagaacatcg ttccgttcat cggcaccgtg gactatctgg tcaacagcgc catgccctac
     5941 gagctgccga tcctcaaggc gcggctgttc aagttcttcc ccgaagccat ggccctctac
     6001 cgcaacgacg ccaagcggca ggacgcctat ctgcgcgcca agcgcgtgtt tgaattcctg
     6061 gaacccatga cagaagtggc cgacgacagc tgcgtgccgc ccaaatcact gctgcgggaa
     6121 ttcatcggtg gaagcgccta caagtattaa aaacctttcg gtcccggatg cgctccggca
     6181 gaacgcaaag cttcatgccg gagcgcccgc gattgtcttc gggggccgaa ccctcgattt
     6241 ccagaccgtg gacgcccagt ccgaccgcgt cgccgccggt ctgcatgcgc gcggcatacg
     6301 caagggcgac cgcgtcgcgc tctactgcat caattccgac ttctttgcgc tggcctattt
     6361 cggaatccag aaaacaggcg cgaccgtcgt cccgatcaac ctgctgctca acccgaagga
     6421 gatcaactat atcctgaagg acagcggggc aaaggccatg atctattttg aggcctttgc
     6481 cggaaacgtc aaggccgcca cggaagatgt ggagcttctc ggccgggctt gcatcggcgc
     6541 aaacaaggcc ggcgaaggcg atttgctctg gccggaactt ctgagcgacc agaagccgcc
     6601 tgcggttgtt ttcaatccgg cagaagatgt cgccgcgatt ctctacacgg cgggcaccac
     6661 gggccacccc aagggcgcga tgctcacgca cacgaatctg gtgagcaatg cgtggagcgt
     6721 ttcgcaggcg cttccgctcg agccgggcaa ggattccatt ctgctggtgc ttccgatgtt
     6781 tcatgcgttc gccgcaacgg cgggaatgct ctattcgatg cttcacggca tttgcgtgat
     6841 tcccctgccc cgtttcgagc cgcacgagac cgccaaagcg ctcgccgcca caaaggccgg
     6901 catgttcatg ggcgtgccca gcatgtacaa tgtgctgctc catctgcccg acgaatgcac
     6961 ggagatgttc tcatcgctga aatactgcat ctccggcggc gcggccatgc ccgtcgaggt
     7021 gatgaaggcg tttgagacac gctacggcaa actgatctac gaaggcgacg gccccaccga
     7081 atgctcgccg gtcacctgcg tgaatcctat tgggggcaaa cgcaagccgg cttcagtcgg
     7141 gctgccggtt ccgctggttg aaatgaaaat catggatgac acgggcaaag aggtttcgca
     7201 cggaaccatc ggggaaatct gcgtgcgcgg cccgaacgtc atgaagggct actggaacca
     7261 gccggaggaa accgccgctt cgttcttcgg ggaatggttc cgcaccggag acctcggaac
     7321 cgaggacgac gacggatact ttttcatcgt ggaccgcaag aaggacatgc tcatcgtcaa
     7381 cgggatgaat gtttatccgc gcgtggtcga agaagtgctc taccagttcg agcccgtccg
     7441 cgaggccgcg gtgattggcg aaccgcacga gctgcacggc gaaatccccg tggcctatgt
     7501 tgcgctgaaa gagggccgca aggccaccgc caacgatgtg cggaattact gccgcagcaa
     7561 tctcggtcgc tatgaagtgc cccgcaaagt attcttcatg gacgcgctcc ccaaaaacgc
     7621 ggccggcaaa atcatgaagc gcgaactgcg cacgcacggc gaactcgaac gcggcataga
     7681 cagccggcgc gacgaaccga aataatttca gcgcagaatg ctttgtaact gcggcaattt
     7741 ctcatacagg cccagcatgc cgcctgtatg cacaaagaga atgctgcgcc ccatgaaccg
     7801 gaagggattc ttactcagct ccttcaccat cgcacaaaag gctttccccg tataaaccgg
     7861 gtccagaatg atgcccgtcg aagaggcaat tccggcaatt tgccgcagtt cctcgtccgt
     7921 gcttacggca tagcccagcc cgacatgtcc gtccatgatg tccagaatat cctccgcctt
     7981 ctgcgaagcg cccatgtccc gcagtatctt ccctattctg tcgtagaaat accgagcatc
     8041 gcgattgaca ttgaccgcat gcaccttcac cgctgccccg gaaagcgccg cgccgagggc
     8101 cattccagcc gctgttcccc cgcttccgca ggctaccacc gcatctgtga tttccagatg
     8161 cgccctctcc atttgaagac gcatctcctc ccatgcgtcc agatagcccc atgcgccgag
     8221 cgcattggac ccgccctcgg gaatgatata cggcctgcgg cccgaagatc gcagagtctc
     8281 cgccagttcc cgcatgacat catccctccg gagatactcc tccggcgtga tcagatggat
     8341 tttcgctccg accagacggt ccagcagcag gtttccctcc aggcccggat ctgccgatgg
     8401 acaatccgtg ctcaaaaaca gatgcgggat cattcccgcg gccacagccg ctgcggccgt
     8461 ggctcgacag tgattcgact gaatgccccc gcaggtgatc accgtgtcgc atcccgcact
     8521 cgaggcttct gccagaagga attccagttt gcggaccttg ttccccgaca gcgcgcagcc
     8581 cgtgagatca tcgcgcttga tccagatatc tgccgccggc agcttccatt tttcgagcgg
     8641 ggtgggccgc attgccagac gcatcacgcg atcgggtgcc gatgaaaggc cggcggccca
     8701 ttccggcgca gcatattgtt caagtgtgat catattttac ttcccgcctc cgatttttat
     8761 gacagttgcc ccgcaaagta aatcaatagt gcatgggcgt ctccaaactt gtcctcacgg
     8821 ggctccggac cgcagcggga gattcccctc ccggcttccg ctttcgttct gttttgattg
     8881 acagagggtg ctgaatatga tacggggaca acggcttatg gcatggaggt caccaaaaag
     8941 cccgggatct ggaggctggc tatgaaagca ccgttgcgca ggatatgtct gattcttctt
     9001 tcaatccttc ttccgcgggg agggccggcg gcagaacatt ccggcaacgg atggatcatc
     9061 cggaagaccg ccaaggaaac ggccgcggaa agcggatcca ccattttcta cgtcatctct
     9121 gtggaacgca ccgggccgga tgccggcgaa gtcaatatcg aagactggcg gggagccgcg
     9181 ggcgggacaa cggcatatct ctgggcaagt cccgagggcg acggcggcgg cggaacggcg
     9241 gattatctga actggacccg gacaatgaat cagggagaca agttcaccgc catctacgaa
     9301 gtcgaagtgg acacgggact ttcggccgga accattatca acaactacat catggcggat
     9361 actgttccat acgatcccga catgcacatc tcgctggatc attacctggt cgtgggaggc
     9421 gggaacaaga cccgaacagc gaaccgcggc tgcggccaga gcagcgaacc ggtcagcacg
     9481 gccacgggag aattctttat cccgccggtc accgacatgg atctcggcgg gcctttgcct
     9541 ctgaaattca cccggtggta cggctcgcgg ctgaatgatc cgggcattga tttaatcggg
     9601 agttcgctgg gcaaaggctg gatgcacagt ttcgaatccc gggccattca cagcggattt
     9661 ttctcgcgct ggctgagggt catactgccg ggcggcagga tcgcgagcct ggcggaagtg
     9721 tatggcggcg aagggggctg gcagcttgcc tacgagcagg aggatgtcgc ctatcagggc
     9781 cgggaagatg gcgtgagttt ctggctcatg gatccggagt ccgaacgcct gtaccgcttc
     9841 gatgcgccgc cctactcctg gggcagcacg aaccggctcc gggaaatcgc ggatcgcaac
     9901 ggcaatgcgc ttctgctgga atatcgggca gatgggcgga ttacaaatgt cgcggacggc
     9961 ctcgggcgga acctctcctt cacctatacc gccacaggca atcttctcgc ggtcagcgac
    10021 ggtgcgcgaa ccaccggttt cggttgcgat gcacaggggg cgctcatttc cgttaccaat
    10081 ccattgggtt atgtcaccac ctgcatctat gatcccgtaa actcccatac caacgggcag
    10141 ggggcactga tgacgggaat tcagcatccc cgaggaaacg tgccctacac acaggcctac
    10201 aactcaaacg gacaggtggt ctcccaaaca agcgcctgcg gcggcatgtc ctctttctcc
    10261 tacaccacaa ccgtgtacgg agagacaaca cagatagatg acccgcaggg gtcactggtc
    10321 cacaaccatc tgaactggtc acgggccacc aatctggtcg accaggccgg aaacggtttc
    10381 ggaatttcat accagaccga gcggaacctg ccgacgcgca tccgcgaccg gtgcggacaa
    10441 gcgagctctt ttcaatacga cttccacagc tgccgcctga ccaacgtgat acatcgcgac
    10501 ggggcgacga ccacttacag ctatgtcacc accacgcagg ttttcacaaa ccgcgaaaca
    10561 ggcaccaaca cggtggaatt cgattttcag gatttgtgcg ccattcgcca tccggacgga
    10621 agcacggaag agttccatcg cgacgcacgc ggcaatgtga ccagttacgt gaaccgcgcg
    10681 ggcgcagtct ggacaacgac cttcaatgag cgcggccagc ccctgacggc atcccggccc
    10741 ggcggcggcg ttcaggtctt cacatacaac cccgacggca cactggcgag cgaggcggac
    10801 agcgataccg gtgccatcat ctacacctac gatgccctgc gccgccgcat ccgcgaaacg
    10861 cgccccaatg gcggccagac ggggttcgcg ctcgatggcc tcgatcgaat cgtcaccatc
    10921 acaaacactg cgggcggcac aacttctttc cagtacgacc ccaacggcat ctgcacaaat
    10981 acggcggagc cggccggata tacccgaatg cgccagaccg acctgatgaa ccgcgtgacg
    11041 aactgcgcgg acagcctggg cccgctggcg gccatctctt acgacagcat gggccggcct
    11101 gtcattgaaa ctgatccggc aggcacaaac cactaccact acgacgatcg agggtggctc
    11161 acaaacctca cccgcgccgg ccgcacatgg tcatggagct acgacccgga gggacatgtc
    11221 gcacaggtcg tctcgccgtc gggaaactgc acggtctatc aacgcgaccc gatgggacgc
    11281 gtcaccaata ccatagacgg gctgggaaac gcgcagtctt cctcgcacga ccgcatgggg
    11341 cgccgcatct tgttcacgga ccgaaacggc ctcgtaacgc atttttccca tgagcccggc
    11401 ggacaggtgg cgggaatctc caacgcgctg ggcgcaactg cttcgctggg atataatgcg
    11461 gacggcctga tcacgacggc gaccgacttc gatggccgca tcacgaccgc aggatacacc
    11521 cccatggggc gcttggccgc catcaccaat gcgcttggcg aagtgaccgg ttatgcctat
    11581 gacacagctg gccgcctgat ccgaacagac tttcccgacg gaactcacga gagcctcgca
    11641 tatgatctcg ccggccgcct catcagccgc acagaccgcg gaacaaatgt ctggacctgc
    11701 ggatacaacg cgcgcggcga tctggtggcc gtgacaaacc cggcgggagg tatcaccctc
    11761 cgcgactatc tttccgacgg gaccctgaag tggatccgcg acagcgacgt gaccaacatc
    11821 ttctttgtcg gtgacggagc ccgcaggctt gtggaagtca ttgtgcccgg cggagcgggc
    11881 atccattacg aatacaatgc ccaccatgag atcacggcca tcgtggatgc aaattcgaac
    11941 agaacagact acgcatatga cagcgaaggg cgcgtggtct cggaaaccga tgcacatggg
    12001 cattcgctcc agtacgagta tgaccccgtc gggcgggtga ccagcatcgt ggaccgcacc
    12061 ggcgcacgga gctgttacga gtatgacgcg gccgggcgac tgacggcggc cgctgatccc
    12121 gctggagtcc gcgtacagta cggctgggat ccggggaacc gcctgacgtc cgccacactg
    12181 ggcggacaga cctggacccg gatatatgac acggccgggc ggctgacggc acattccacg
    12241 ccgctgaacc gaacaacctc acttatacgg gatgcggcgg gacgcctcgc aggccacgtg
    12301 gatcccatgg gccgcacgaa tctcattgcg cgcgacagca tgggaaggat cacggccgtc
    12361 accgacccgg cgggccgggc ccgagtgttt tcctgcgaca gccgcggcct gctggtcgcg
    12421 gcggccgcca cgggccaggc ggccgcccgt tatgaaaggg atgcgcttgg gaacgtgatt
    12481 cgcgttacgg atccgaactc aaacgcatgg actttttcgt attcgtccat gggggctctg
    12541 ctgggcatgg cggatccgct gggccgaagc aatgtcctta tgcgcaatgc ccgcggccaa
    12601 atcagcggga tattgtatcc ggacggcgcc gccagcacca cgcattacga tccggccgga
    12661 aatgtcaccg ccgctgttta ttccacgggg ctcattctcc cgtatgcata cgaccaccag
    12721 tcgcggctga tcggggccga cggaatcgcg ctacagcgtg atccggaagg ccgcacgacc
    12781 aacacgattt ccacttctga cggacgggcg tttggcgccg catacgacgg cgcgggccgc
    12841 ctggcggcgg catattatga cggcgcgctg actgttacgt acatctacaa caccacaaac
    12901 ggcctgctgg aatccgtttc agattcgtca agcggcgcgg cggtgtggtt cagctatgat
    12961 gcggaccggc ggctgacagg cataacacgc ggcaacggac agcatgcagc cttcaccctt
    13021 gacgacgccg gccgcatcac ccgaatccag gatgggacac tgacagatgt acagtatgaa
    13081 tatgatgcgg cgggccgact gacagccgaa gaagggacat atccgctgga ggccggcgca
    13141 aatcttgacc ctgaaacgaa ttccttcaca tatgaccccg ccgcccagat ttcgtcttct
    13201 ggtttcgttt acgatgcccg cggacggctg accaatgctc ccgggcgccg ctttgtatgg
    13261 gatgccgctt cacgcctgat tgatgcggac ggcatacaga tcgcatatga cggatttcat
    13321 gacgtgcttc agaagaccgc cgccggaacc acaatccgct actaccacaa ccgcgcggtg
    13381 aggcctgcct ccatcgtggc cgaacgcgat gaaaccgccg gtacattcgt gcgctactat
    13441 gtgtggaccc cggccgggca gctcctctat gccattgaca cggcctccgg caacaagcct
    13501 cgctactacc acttcgaccg gatcggttcg acactcgcca tcacggatga aaacggccag
    13561 gtgaccgacg cgtatgccta cacgcccttc ggaaggattg tgcgccggca gggcgcgtcc
    13621 acccagccgt tcactttcgt cgggcgccac ggtatccgcc agcaggacgc cgacggaacg
    13681 ctctaccaga tgcgggcgcg atactacaac gcacaaaccg gcggcttcct gtcacgcgat
    13741 cccggctggc cggaactttt cgatgcgcgc aagatcaacc cctacgccta tgccggcaac
    13801 gatccggtcg gccggatcga ctgcaacggg cgctggtgga tggggcccga cattgatccc
    13861 ctgccggatg atgctgatgt gaccatgctg tcgaactggc agctcacgga aatcgaactg
    13921 cgcctggagg aaatggaccg ggatgacaac atcaacctgc cggaggttcg catccggaag
    13981 gaccgtatcc gggccgaact tcaccgccgc aaccccattc cgctgatcat ccccccgaag
    14041 ctgcaggatc aggagcatac ctcgtgcgaa cagcagccgc tcaacacggc tgttcagccg
    14101 gccggacaga tggtggaaac gccggggctg acaggcgaga ttcctccgcc gccggcccgt
    14161 tacagaaaac cgctggacca atggacgccg ttcgatctgg cgcacgagat attcagcccg
    14221 gagaacatgc agcgcgcggc cgtcgaacag cacatcgcgg agcgcgaggc ccgcgagcgg
    14281 gcggagaagg aagcagccga acgggctgaa gtcgaaagga cggcgaggga ggccctcgaa
    14341 cagttcatgc ggcggacaca aggcgttcct ccggaggtga agaagcctca gtatacgctc
    14401 cccgaatctg tcttcaagcc gacaccgcgc ccgttccgtg accctctgga attcattctg
    14461 gacagcctgt tcggcccgca ggccattttg gattttgccg cgcagcagca gcttcagcag
    14521 caggtccaat cccaggcgca gacagaagca tctaagccgg cggcagctcc ggagaagccc
    14581 cgcataccga cccgcgatct gtaccagaac ctccagaaag cccagcggcg ggcggcggag
    14641 ctgatgcagg cgcgtgattt cgaaggcgcg cgaagggagc tggacgacgc gcgccgatgg
    14701 gaacaggaaa tccacgaacg ctaccagcgc gaatggttca ccatgtatcg ggactaggga
    14761 gacagataat gaagaatcac aagatctgca tgcaaaaggc tggaaggctc ctgctgacag
    14821 gaatactgac gggtatgatc ctgtcaattc ccgcatatgc agcgaggatt gattatgcct
    14881 acgacgagac cgggcggctg acaaatgcag catacagcgg cggggccggc atcgcctatg
    14941 cctacgatgc caatgggaat atgcttatcc gcgaaacatc cgccggcggg gcggccacct
    15001 acacactgat ttatctggct ggagcgggcg gttccatcgc cggaattgca acacaggaag
    15061 tcgcggcagg agacagcggg tcagcggtga ctgccagccc ggccgacacc agcgtggtct
    15121 ttcacgcatg gagcgacggg ctcttcgaca atccccgcac agatacaaat gtcagcgcca
    15181 atctcaccgt cagcacggtc ttccgcagcg aaggcggcgc caatctggac tggtacgccg
    15241 ggcacggttt tgtacccgaa gggggcgagt tgtggagcga tctcgacaca aagattgcct
    15301 cggggaaagg cacaaccctc cttcacgagt tcatcgccga caccgatccg aataacacag
    15361 gtgatatctt ccgcgtgctg tccatctcaa acggcccgcc ggtggtcgtg cagttccagc
    15421 caggctcaac cggccgggta tacaccttcc aatatgctgg agatctggcc ggggatgcct
    15481 ggaccaatgt ccccgggaca gttccgcgtc caggcctcgg gggtcaggat gcaatgaccg
    15541 acaccaatcc cccgcccttt agatcatatc gaatagaggt tcaggctcca taaacagctc
    15601 cggaagcagt ttccacgcct gcccgcttgg catctcctga tgcatgtggc atgatggccc
    15661 gcgattcaag caggcaatta tatgagcaaa aaacagaaat acatcaaact gcagaaatcc
    15721 cggaggacca gaggtgtgat tattgccatt gtccttttca ttattctgct cccttacgca
    15781 gtctgctggt gggaaagaca tcctgccggg ttcagtgtgc gctcgaaacc ttgcccggtt
    15841 ccggcggaca gcgttcggct tctaattgac tccacgtcat tcgatgccga gaaaggcgaa
    15901 cggattttcg accagcagat tttcgatgcg gtgctgaaga tgatcaggga tgcgaagcag
    15961 actgtgtatg tcgatttctt cctgtggaat tactggactg gatccatccc ggaaagtcat
    16021 cgcggcctgg cacaggagct gtccgagtcg ctgattgcaa agaaaaaaga atcccctgaa
    16081 acgcatatcc tggtgatgac cgatccggtc aaccgcgttt atggaggaga tgaacagccg
    16141 ttctatgaag ccatgcggca ggccggaatc tgcatcgtct tcaccgccct ggataaaatg
    16201 ccggaatcga atttcttcta tgcggcaccg gcccgttttt acgggccgct gatcagccgc
    16261 attcccggca tccggcagtt gctgaacatc cgcctgctga aacatccctt tgaagaagga
    16321 ggccagaaca tatcgcttct gcagttcggc cggcttcttt tcttcaaggc caatcaccgc
    16381 aaggtggtca tcacggacgg ccccggcggc tggcagctgc tggtcaccag cttcaatccg
    16441 gccaacggga gttccggcca ttccaatatc ggcctgcagg ccggcggaga gatcgcacgg
    16501 gaagcgctgg ccacggaact gcagtgtgtg aaatggtcgg cggagaatcc gggcaacgtg
    16561 ctggaacccg aacccggagc ctgtgcgctg atcatcgggg ttctttcgga acgcctgtct
    16621 gaaccccctc tgtcatcgaa ggccgacgag aagatgaccg cagaatggct gacagaagaa
    16681 tgcatcgccc ggaaggccga agcccttctc gacgcctgcg gcacgggcga cgaggtgcgg
    16741 ctggcgatgt tttatctggc cgaccggaaa ctgatcacag ccatcaggga agccgcggac
    16801 ggcggggcgg acgtccggat tatccttgat gcaaacaggg acgcctttgg gcgggtgaaa
    16861 aacggcgtcc cgaaccggcc ggttgccgcg gagctgatgg agcatgccgt tcgtgaaggg
    16921 gttcggctgc agatcaggtg ggcggacacg catggcgagc agttccatcc gaaagtgctc
    16981 tccattagca atgcggaagg caaagcggac ctgctgtgcg gctcggcgaa ctggacacga
    17041 cgcaatctgc gcgacctgaa tctagaggcc aatttgcacc ttaaaaacgc gccggaagtc
    17101 tcccgcgcgt tcggcgaatg gtttgatctg tgctgggaca atgccgatgg ccggagccgc
    17161 acggtcccat acgaggtgtt cgccgtctcc ggatttgagc gttcccgcaa agccctgatg
    17221 gggcgttttc aggaagccac cggcatgagc acgttctgag ccggaagagg acgatacgga
    17281 ggcaggccct ccagagccgt cttccggctt caatattccc gtttcatcag tgaccgcagg
    17341 cccagccaga gagggaccag aaccgccgcc agtgtgatcc ccaccagcca ggcgcctcca
    17401 agcatcagcg catgaacaaa catttccgga gacagatatc cgcggtaata gagctgaaac
    17461 acaaatgcaa acgggaacat ggtcatgaag atgaaaacca ggctgcacac caggttgagt
    17521 gtgcccccga agctcgagac aatggcggac ggattggaat gcttgaggtc catgaacagc
    17581 gcgcccaggc cgcaggagaa gcccgaaagc gcaacggaca ccgcaaaggc caccgccatg
    17641 ctgaccatgc ccgcataggc ctcgatgttc agcatgcgca cacagattcc catcagaagc
    17701 agactgacga agaccatgct gaacagcgcg ccggcgaatt tcgagagcag tatccgtttc
    17761 atggacagcg gagacagtcc cagcacccag aaggcgtgac cctcaaagct gagctgcgga
    17821 tagacaaatc gggcacccag tgaagtcagc acggccgaca cgctgaacac attaagaaat
    17881 gctataagat tccgaaaggc cagcggaagc gtgtgataac ggagattgcg cagattcaga
    17941 aaatacagcc cgagcatgcc gaggaaaaag agcgcctgca cccactgggc cgggtcgcgc
    18001 agaaagatgc agatatcctt cacaaggatg ctgcgcatgt cgcgggggag aaacaccagc
    18061 gtccgctcca tgactgcgcg cgcgcggtgc atgcggtgat gccgcctgga accccgcatc
    18121 agcttctggt atccctcttc gtaaatccgg gagcccgcgg cctccagaag gacggagccc
    18181 atccccgcgc tggaaaacag gaggagcccg aacatcagcc ccctcgccca ctgcccgcgg
    18241 ctgatggaca ggatgccctc ggacgcccag tagccgggaa gcaggggatg cgaggccagt
    18301 ttcagaccgg gaatcacgcg gttgagaatg acattcacat cctcgcccag ccggacattc
    18361 accacaaagg ttttccagcc ggcgatgccg accgccagta tgaccgccat gagaatatat
    18421 ttaaccatgc ggcgcggcgg catccagcgg atgaagacaa gacacagcag cgccccggcc
    18481 gaagaacaga tcatgacaaa gggcagcgag aacacaacgg atgccagcag ggcaaacccc
    18541 gaaagcttct gctgcatggc aaaggccatc acaaagggca cgatgatgaa aaaaaatgcc
    18601 catgaggcat aaaatgcggc gtgcagaaat ttataccgga tcagcgtccc gatgcgaacc
    18661 ggcatcagtt tcagaaagcc catgtcccgg tcgccgaaaa aagttccgta cgccgaaacc
    18721 gagctggaga gaatcagcag aaaagtcagc gcgaggaagc agaaggccag cagacgatga
    18781 atgatcatca ggccgattcc accgaggcgt tcaagaaagg tgaacccctc ccagaaaagg
    18841 ccgtacagcc cgcccagcat tcccagcgca aaaaagagta tgaccgccag cttgaaacgc
    18901 gactgttcag ccagttcgtg gcgcagattg gccgcctggc gccagtcctt tttcagcagc
    18961 atgcccagtt cgctcaaatc gcggcactcc ttattccgta agtttgagga aaacatcctc
    19021 aagccggccc tgcatgtccg tctgccgcat cagttcatga agcgttccaa gggcgaccag
    19081 ccggccattg tcaatgatgc cgatccggtc ggccatgtct tcggccagcg cgagaatgtg
    19141 cgtggtcagg aaaatggtgg cgccggcggc gcatttttca cgcagcaggt ttttaatgag
    19201 ccggatgctg taggggtcga ggccgatgaa gggctcatca atgaacagca ccgcgggatc
    19261 gtgcaggaaa accgaagcat agcacagccg ctgccggatt ccgtgcgaca tgtttttgat
    19321 cagagagtcc tgatagtcct cgagtccgaa cagttcgaaa gccttctctc gcttcgcgcg
    19381 gatttcatcg cgtggaattc cgtacaggtc cccggtgaat tcataaaact cggcagcggt
    19441 gaggcgctca tagagatagg gctggtcggg gatgtaaccc gtgatcttct ttacctcctg
    19501 cgcgtgctca tggaggtcca tgccccggat cacgatccgt ccggagccgg gtttcagcaa
    19561 accgcacagc attttgatgg tggtggtttt ccccgcgccg ttcgggccca ggaaacagaa
    19621 aagttcgccg gccggcacgc gcagatccag tccggccacg gcatgcacgt cgccgaaagt
    19681 cttgcggagg tcttcaatta caatcattgg gggatgctca ctcatagatt gcctcgagaa
    19741 tatccgcctt cacctgcccg accacctgca gcaggcgcac ctgggcggcg gattctccct
    19801 cgcccagaaa aatatgagag gcatcggctt catgctggcc gaatgtggga tccatatcca
    19861 cccagcggcc gacaaacacg ctgggccatg catgatagta gaaagccccg tccagataaa
    19921 ccacacccag gtgaatgcgc gcgggaatgc cggcggcgcg ggccagcgcg acaaacaaat
    19981 aagtgtgctc attgcagtcg cccgctccaa cacgcagcac ttcgatgccg tccccgatcc
    20041 ggcgcgctat ctgtcggcca cgccctttgt gcagtcgg
//
LOCUS       DAOQWR010000032        19906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3082_length_19906_cov_6.120195, whole genome shotgun sequence.
ACCESSION   DAOQWR010000032 DAOQWR010000000
VERSION     DAOQWR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_19906_cov_6.120195"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..576)
                     /gene="cas2"
                     /locus_tag="PKK36_04105"
     CDS             complement(283..576)
                     /gene="cas2"
                     /locus_tag="PKK36_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNR93776.1"
                     /translation="MLVLVSYDVSTMDKRGRRRLRRVAKICQDCGQRVQNSVFECIVD
                     PAQWTALRDRLLAEYADEEDSLRFYFLGSNWERRVEHHGIKVVPNLEKDTLIV"
     gene            complement(621..1652)
                     /gene="cas1c"
                     /locus_tag="PKK36_04110"
     CDS             complement(621..1652)
                     /gene="cas1c"
                     /locus_tag="PKK36_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HNR93777.1"
                     /translation="MKKYLNTLFVTTQGAYLNKEGESVVVKIEQEIRLRLPVHTLDGI
                     VCFGAVSMSPFLMNHCAENNVAVSFLTEYGKFLARIQGPVSGNVLLRREQYRRADDGG
                     AAADIARSFIASKLANSRTVLLRALRDHPAKTDDVVIGDAENRLSQYIQRLKKPQAAE
                     SLRGIEGEAAASYFSAFPQLITADDNAFAFTGRNRRPPLDPVNALLSFTYTLLVHDCR
                     SALEGVGLDPCVGFLHTDRPGRPSLALDMMEEFRSCLADRLVLSLINRKQVKAADFRD
                     AGSGAIVMTDSARKELLTSWQKRKQEEITHPWLGEKVAVGLLPHIQSQLLARHLRGDL
                     DAYPPFIWK"
     gene            complement(1636..2289)
                     /gene="cas4"
                     /locus_tag="PKK36_04115"
     CDS             complement(1636..2289)
                     /gene="cas4"
                     /locus_tag="PKK36_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNR93778.1"
                     /translation="MYSEADLLPLSALQHFLYCPRQCALIHIEQQWGENRFTAEGRAQ
                     HDRVDRRESEIRDGVRMEYAVPLRSLRLGLSGIADVVEFHSDLKSEILNLKSPVRAYP
                     VEHKRGKPKPSDCDRVQLCAQAMCLEEMLNTEIPEGAIFYGQPRRREVVLFTPQLREE
                     TERTVTGVHELLESGKTPSAEYNEKKCTACSLLDICMPRVRKNASAYLREIIDEKIS"
     gene            complement(2289..2801)
                     /locus_tag="PKK36_04120"
     CDS             complement(2289..2801)
                     /locus_tag="PKK36_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR93779.1"
                     /translation="MMKYENFEDVPVWQDAMDLSARIFEMTEDRAFQGRGDIANQIQR
                     AGLSISNNIAEGFERGTTAELIQFLYYAKGSAGEVRSICHVMDRLKVFEHLKSQISDL
                     RSEARSISKQIAGWAFSLQESDIKGSRHLTEQSKKTYHKGKNAKNLLDDLKKQHEDRL
                     EQWKRKREGS"
     gene            complement(2852..3712)
                     /gene="cas7c"
                     /locus_tag="PKK36_04125"
     CDS             complement(2852..3712)
                     /gene="cas7c"
                     /locus_tag="PKK36_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HNR93780.1"
                     /translation="MSDPIMNRYEFVLLFDVKDGNPNGDPDAGNLPRIDPETGNGLVT
                     DVCLKRKVRNYVGLTKGEQPPFEIYVKEKAVLNRAHERAYQAFELKPESKKLPKGVED
                     AKKVTGWMCQNFFDIRTFGAVMSTEVNAGQVRGPVQFGFARSVDRVFTGEHSITRMAV
                     TKEADLEKERTMGRKFTIPYGLYRVHGFISAPLAKQTGFSEEDLMLFWDALINMFDHD
                     RSAARGEMAPQKLIVFKHESELGNAPAQKLFDLVKVEKICGDNPPRSFADYKVTIEAA
                     PAGVAVDVKI"
     gene            complement(3709..5460)
                     /gene="cas8c"
                     /locus_tag="PKK36_04130"
     CDS             complement(3709..5460)
                     /gene="cas8c"
                     /locus_tag="PKK36_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNR93781.1"
                     /translation="MILKALADYYDRLFHDDSADVPPYGFEDKGIPFVIIIGKDGRFL
                     NIRDTRTGTGKQQSAREFRVPLGEKKTSGSKANLLWDNPQYVLGVPKSEKAKDVKKAK
                     DDHKVFLARLHKDLDGMTDDGVSAVFRFYETGGIDGCRQHALLSDIMEHNSNITFALD
                     DEVGLIAQRPNVVERIRAIYEEVNGRSCASAISGEIDEVAVLHAAIKGVWGAQTSGAN
                     IVSFNDEAYCSHGKTKKDQGLNAPIGKHNQFAYTTALNRLLASENQRMQVGDASTVFW
                     AKNPCDFEQDFHSFLAPPKGEESVSYDKIRGLLSAVKTGIPPREADLPFYVLGLAPNA
                     SRIAIRFWYDGNVKEIKERVAQHFHDLELVRAPHDPEFLSLFQLLVSTATERKADNIP
                     PNLGGDVARAVLTGIAYPRTLFANAIRRCKAEQSVGFARASIIKAFLVRNARISKSNQ
                     KEVSMALDKTYDNIGYVLGRLFAVLERIQEQAQGKGLNKTIRDTYFGAAASSPLVTFK
                     RLQDLAIHHLAKIRNSGKGTVWLEQLLQEVTGLIPAQGLPTTLTLEDQGRFSVGYYHQ
                     RQDFFAKKETEEKGEEQ"
     gene            complement(5457..6119)
                     /gene="cas5c"
                     /locus_tag="PKK36_04135"
     CDS             complement(5457..6119)
                     /gene="cas5c"
                     /locus_tag="PKK36_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HNR93782.1"
                     /translation="MNKSNPFRLKVSGENACFTRPEMKVERVSYEVMTPSAARGILEA
                     ILWKPAIKWNILQIDVLNPIKWESVRRNEVGAVISARNIQTAMNSGSGDLGLYVEDQR
                     QQRAGLFLRDVAYVIHAEFEMTEQAGTEDSVTKFEQMFIRRASAGQCFHRPYFGCREF
                     PVAFEFIPKEADPLPTIGTTKDLGWMLYDMDYSGEEPMPQFFHAQMENGSINLRNAEV
                     RS"
     gene            complement(6131..8383)
                     /locus_tag="PKK36_04140"
     CDS             complement(6131..8383)
                     /locus_tag="PKK36_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNR93783.1"
                     /translation="MPDPERKYYAHSLEGQPPEKWQPLDEHLKNVATLAAEFAGPFGG
                     QEWARLAGLWHDLGKYSNEFQKKLYDANGIESHLETKPGTVIHSQAGGHLAQSVMHGG
                     VDRIFCWLIMGHHAGLADFSSAETGAKALEPKMRNPSESTDILKNVPTEVKDQPEPPP
                     PKMLLQNPRADISFLIRMLFSCVVDADFLDTESFMDSDKKKLRQADYPKLSDLLEAFD
                     RHVNGLCRDADETDVNRIRAAVLARCREMADCAPSVFSVTVPTGGGKTLSSLAFALRH
                     AVKHGKRRVIYVIPYTSIIEQTASVFRNIPGFENAVLEHHCNVLDDDETKETVRQRLA
                     AENWDAPIIVTTSVQFFESLYASKTSRCRKLHNIADSVVIFDEAQCLPPQYLRPVVSA
                     IRELHRYYGVTPVLCTATQPVLTKTDSFDFQFREGFESVTEIIERPDRLTGELQRVHV
                     ERFSNLDPVAYEQIAESIQTEGQSVLCIVNRKQDARTLSLMLPEDQTVHLSTNMCAEH
                     RFQTLEKIRQRLPDRNNPLFVVSTSLVEAGVDLDFPVVYRALAGLDSIAQAAGRCNRE
                     GKLPNLGKTVIFVPQNQPAYVLSPASLAMEHLRDDRLSQIFFPETFSRYFAQRFFQLG
                     EQSLDEKNIVGLLGKHLEYSFRTASERFRLIDDGWQLSLIVPFGESPEVLDKLIEWDA
                     RRLFRKLQRYTVSVPKKVMWQLLDENHARELPEYPGTYYLLNKGLYTDRFGFIPPDEL
                     DGYEPDTTII"
     gene            complement(8575..9495)
                     /locus_tag="PKK36_04145"
     CDS             complement(8575..9495)
                     /locus_tag="PKK36_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR93784.1"
                     /translation="MKSPLYRIAAFDGGGIRGIYTATLLARLDKEEPGFLKNVKLFAG
                     TSTGSILAVSLAAGRSPAELAELYRKQGGEAFEDSRLDNVRDMGRLIGADFSEQGLKK
                     ILIRLFGSATLGDLPRKVVIPAFDLGGTPGRGDGCWKPKFFHNFSGPDSDAGEKIVDV
                     IMRSCAAPSYFPTYQGFIDGGVAANNPSMAALTQALDRRSKTRPPAMEHIRLLSFGTG
                     LNRQFIRGQRLDWGYAQWARPIIRLMMDGMMGVADYQCRQLLGAHYHRLAPALPKPVP
                     MDAVRQTAALVRYAGQVSLTDALAWLRKYF"
     gene            9521..9676
                     /locus_tag="PKK36_04150"
     CDS             9521..9676
                     /locus_tag="PKK36_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93785.1"
                     /translation="MAKKWQKIKKKRQKWAKNGDFSQKNGKNAVKREIFGVAGFGFQT
                     VDTGAGA"
     gene            9713..10210
                     /locus_tag="PKK36_04155"
     CDS             9713..10210
                     /locus_tag="PKK36_04155"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNR93786.1"
                     /translation="MAFLIAMSGDHKGDRYEIETDEVTMGRKPDCTIVLDNPTISGHH
                     CSILRKDGLYTLQDMNSTNGTRLNSQRVTGTAPLKAKDLIQAGSLEFMIDGDDIRLPE
                     TESFAEVEVAEGAAVAPDSFANISPFGTKHRESKGLSFTIAMILAVIAAAALAFFIFA
                     LITAV"
     gene            10220..10864
                     /locus_tag="PKK36_04160"
     CDS             10220..10864
                     /locus_tag="PKK36_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93787.1"
                     /translation="MDRRPSLKRTAAFTLIETMLSIAVGALVILLVFSIYHTTMQVVR
                     GQAVRQERSGRAHDALREISRRLACAMEYTAPETVSFSVLPAEESKTGFPELAFISAE
                     AAPGLRREWYRPVAVKITCETDGRNSRLVETLKPMSGPVTTNTTVLAEGAAAFSVRVY
                     NGEEWLEKWPQPEQPVLPRAARIRIEFPPETGRDPAETEVFIPAGNAVSPSEPR"
     gene            complement(10868..12070)
                     /locus_tag="PKK36_04165"
     CDS             complement(10868..12070)
                     /locus_tag="PKK36_04165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93788.1"
                     /translation="MRILFLTSQLPYAGIAGGHSLVYERMLRLGARGHEIGLASFCDE
                     TGREHVPELKDRLYELELQPMPPQPPLLSKFVQTYIGGVPLPFVHPRSIAMTRLVGEM
                     VERSRYDVVVAEFTVMGQYLFWNRWLNAVRRVISCHHSPAIMSAHQLELLGTGPLALK
                     KWFKMYRLIDYECRLYRNADRVLTLSREERYALLNLDPTLRISVVPSGVDASFYHPPE
                     ESPREEAIIFTGNYLDPANEDAVINFTQTVWPRLREKLPGLRFYVVGPSPPVYVRAQA
                     ARDKRLVVTGRVDDIRPWLHRSQVFVCPVRLGRGMRGKILEAMASGLPVVSTSLGVEG
                     LPAQMGDNCLIADTPDIMFNQIHILLEDHALRSRLAHNARTMVNERFRWRRVIPQLEA
                     VLEDVLRS"
     gene            12239..13339
                     /gene="ilvC"
                     /locus_tag="PKK36_04170"
     CDS             12239..13339
                     /gene="ilvC"
                     /locus_tag="PKK36_04170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNR93789.1"
                     /translation="MAKINFGGVMETVVTAKEFSLAKARKILKNEVVAVIGYGVQGPA
                     QALNMKDNGIKVIVGQSKQYPKDWERAKKDGFVPGKTLFEIEEAAKRGTIIMMLVADA
                     AMKPVWKIVKKHMTAGKALYFSHGFGLVYNHLTTVVPPKNVDVLLVAPKGSGTSVRRN
                     FLAGVGINSSFAVEQNATGRAVDRCKAIGIAIGSGYLFPTTFEHEVTSDLVGERGVLM
                     GALAGIMEAQYDVLRKNGHTPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPKFKKAVLPVFRDLYKAVKTKKEAARVVKSCGKKNYKDQLTKELAVIGNSEM
                     WRAGAAVRSLRPKRKAVVITKDTKGVAGRKGN"
     assembly_gap    13344..13443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13646..14326)
                     /locus_tag="PKK36_04175"
     CDS             complement(13646..14326)
                     /locus_tag="PKK36_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HNR93790.1"
                     /translation="MNSTPHRPSETAWGAIFDWDGVIVDSAPLHQLSWEIMAEKERKT
                     FEPDAFVRGFGMTNEVIIPNILGWASDPVEIRRLAREKEETYRRLVREKGLSALPGVE
                     VFLRRLQDAGIPCVVGSSTERANLECAIDVLGFREYLPQMVAGDEVKHGKPDPEIFLK
                     AADKIGMPPARCVVFEDAVAGIQAARAAGMKVVAVTTTRKAEDLHEADIIVNQLNQLS
                     TNAIAKLL"
     gene            complement(14323..14865)
                     /locus_tag="PKK36_04180"
     CDS             complement(14323..14865)
                     /locus_tag="PKK36_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNR93791.1"
                     /translation="MKATDILKREHQAILLAVAAAEEEAAYIADTGKIHLQKVREMAD
                     FFRNFVDRCHHAKEEKHLFAVMHEHGIPFENGPLAVMLHEHEQGRECVRAIANAVAPR
                     EPDEQARRKAGDNLALYARLLREHINKEDHVLYPMADQLLNTGDQDRLRKAFDRVESE
                     ELGEGVHDKYHAWIKKLAER"
     gene            complement(14934..15686)
                     /locus_tag="PKK36_04185"
     CDS             complement(14934..15686)
                     /locus_tag="PKK36_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNR93792.1"
                     /translation="MLKVSKVRLQQGYRTMKWPDGPAPEMPPHFTGRPVISGDCCNGC
                     GDCAKVCPTGAIAVADGKISLDTGLCIFCGKCEKACSKDAIKFGNDFRLASAKREDLV
                     VQPGNDMTPNAYSEAIHKLFGRSLKLRQVSAGGCNACEADTNVLGTLAWDLGRFGIQF
                     VASPRHADGILVTGPVSKNMELALKKTCEAVPAPKIVIAVGACAVSGGIYRGQEETHK
                     GVAEIIPVDLFIPGCPPHPATILDGLLRLLRK"
     gene            complement(15696..17177)
                     /locus_tag="PKK36_04190"
     CDS             complement(15696..17177)
                     /locus_tag="PKK36_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNR93793.1"
                     /translation="MSGPAVIRNGGKLPLSQIPVLTVSDFRRDILDSVSKGARISALF
                     GSPTGENIRLIAVLAWRDTGSISVLSAPVQKSYPALTPDCPQAHLFEREIFEQWHVMP
                     EGHPWLKPVRFPDSEKTSGIGVTNFFQIEGEEIHEVAVGPVHAGIIEPGHFRFQCHGE
                     TVHHLEISLGYQHRGVERSLVNGPNSKSLHFIETLAGDTTVGHATAFSRIVESLAGIR
                     APARAQVIRGIALELERMANHIGDLGALAGDIGYLPTMSYCGRIRGDLLNLTAMLCGN
                     RFGRRLVVPGGVGYDIDSENADKIAARLKSAFRDAESAIGLLWETPSVLARFENTGAL
                     SRETAVKIGLVGVAARACGLQRDVRSDFPYGIFLLSHIPAATWESGDVFARAFVRWQE
                     LQRSYKFILEQLAALPDGAVRAGENPPIASGHLAVALVEGWRGEICHVAITNNEGRFL
                     TCKVVDPSFHNWFGLALALRGQQISDFPLCNKSFNLSYCGHDL"
     gene            complement(17174..18655)
                     /locus_tag="PKK36_04195"
     CDS             complement(17174..18655)
                     /locus_tag="PKK36_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNR93794.1"
                     /translation="MSAENTLPILILAPLIFGGISLLLRSARGALRFCAAGTLATAAL
                     AGITAYNVLRGGPAETANGWLMLDALSAQHLLAMITVFCLSSLYALIYFREEAADGRL
                     TPKMAGRYGGLWFGSLAAMMLVLVSNSLGMMWVGVEASTLMTAFLICIHASPGALEAM
                     WKYLLMCSVGVAVAFTGILLVAAATGPAGLHGMDALLWTTLMDAASRLNPMMLKAGFI
                     FIVIGYGTKAGLAPLHNWLPDAHSQAPAPVSAVFSGFLLNAALYCILRCLPLVEAATG
                     NEGWGRSILVIFGLLSILVAAAFIVMQRDVKRLLAYSSVEHIGIMALGAGIGGAGAAA
                     ALFHLINHSICKPIAFFCAGRIGQIYGTHDLPGLTMIIRRAPVWGAGLIISLLALVGV
                     APFSVFLSEFLVLKAAVDHSLYVVAALFLLGLGVVFIAVLRHIIAMAWKPAEQQAGPV
                     KFSLPEALLIFIPLAIVLLLGMWIPGPLLKILSRAAQVLGGLS"
     gene            complement(18658..19302)
                     /locus_tag="PKK36_04200"
     CDS             complement(18658..19302)
                     /locus_tag="PKK36_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNR93795.1"
                     /translation="MIILLDTLLVTLILTGFLLLGSSRLGASIRISALQGIMTGLLPL
                     ALEHGDLFSYVSLLGVAVIGLKGYVFPRLLFSALRESDTSREVQPFVGYMASLFFGIL
                     FLMGSFWLDTRLNLPVHSSSDLLVPVAFSLMSCGLFLIVARRTAMSQVIGYLVLENGI
                     YCFGLAVVSGMPLLLELGVLMDIFVAVFVMGIAIYHINREFDHIDADRLSALKG"
     gene            complement(19299..>19906)
                     /locus_tag="PKK36_04205"
     CDS             complement(19299..>19906)
                     /locus_tag="PKK36_04205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNR93796.1"
                     /translation="FGLLRFFLVAAALDTGSAFEGMGASREVQFSALAEPALLLGLAA
                     LARATGSLSLTGIFSASAGPSAGPALALVSAALFIVFLAENARIPVDDPNTHLELTMI
                     HEVMVLDYSGPDFAIILYTSSLKMWALGALLVNVLLSFLPLHGPAAIAGVAGGMILLS
                     VLVGIVESIMARLSLLKVPQLLAGAVAISVLALTLVLRFTS"
BASE COUNT         4351 a         5605 c         5696 g         4154 t
ORIGIN      
        1 gcgatcggca gtttcaatcc acgcccccgc gcgggggcga caagatatgc cctatatcca
       61 gtttcaggtt ccgcgtttca atccacgccc ccgcgcgggg gcgaccgcga aaacataact
      121 tattgtccat aagcacacaa caagagagaa tccgcgaatg ccacattctg cgtcggcatt
      181 attctttttt caaagagcag ttcttcataa gcgattcatt tgaaatgaat tacattcgtc
      241 gcgaatacgg ccgcattttt gaccagactg gaggttcgcg attcatacga tgagggtgtc
      301 tttttcaaga ttgggtacca ccttgatgcc gtgatgttcg actcttcgct cccagttgct
      361 cccgagaaag taaaagcgca ggctgtcctc ttcatcggca tattcggcca gaagcctgtc
      421 gcgcagcgcg gtccattgcg ccggatccac gatgcattcg aagacggagt tctgcacacg
      481 ctgaccgcag tcctggcaga tttttgctac gcgccgcaac cgtcgccggc cgcgtttgtc
      541 catagtcgaa acatcatagc tgacaagaac gagcatgaca tcctccggat gatttcaaat
      601 tagaaatctg agatttcata ttatttccag ataaaaggcg gataggcatc cagatcaccg
      661 cgaagatgcc gcgcaagcag ctgcgactga atgtggggca acagacccac ggccaccttt
      721 tcccccagcc atgggtgcgt gatttcttcc tgtttcctct tttgccatga ggtcagaagt
      781 tcctttctgg cggaatccgt cattactatg gctccactgc cggcatcccg gaaatctgcc
      841 gccttcacct gttttcggtt gatgagcgag agcaccaggc gatcagccag acaggagcgg
      901 aattcctcca tcatgtcgag cgcaaggctg ggtcggccgg ggcggtcggt atgcagaaat
      961 cccacacacg gatcaaggcc gacaccttcg agagcagaac ggcagtcatg taccagcaga
     1021 gtataggtga aggagagcag tgcattgacg gggtcaagcg gcgggcggcg gtttcgccct
     1081 gtaaaagcaa aggcattatc atctgcggtg atgagctgcg ggaatgcgga aaaatatgag
     1141 gctgcggcct cgccctctat tcctcttagg gattcggcag cctgcggttt tttgaggcgc
     1201 tgaatatatt gtgacagccg gttttcggcg tcgccaatca cgacatcatc tgttttggcg
     1261 ggatgatcgc gcaatgcgcg cagaaggacg gttctggagt tggccagttt gctggcgatg
     1321 aatgagcggg cgatatcggc ggcggcgccg ccatcgtctg ctcggcgata ctgttcgcgg
     1381 cgaagcagaa cattgccgga aacgggaccc tgaatgcggg ccaggaattt cccgtactcg
     1441 gtaaggaatg aaaccgccac attgttttca gcacagtggt tcatcaggaa tggactcatc
     1501 gaaaccgccc cgaagcagac aatgccgtca agggtatgta cgggcaggcg caaacgaatt
     1561 tcctgctcga ttttcacaac tacggattcg ccctccttgt tcaggtatgc gccttgtgtg
     1621 gtgacgaaga gggtattaag atattttttc atctataatc tcccgaaggt aagccgaggc
     1681 atttttgcgc acccggggca tgcaaatatc cagcagcgag caggccgtac actttttctc
     1741 attatattcc gcagatgggg tttttcccga ctccagcagt tcatgcacac cggttacagt
     1801 tcgttctgtt tcctcgcgca attgcggggt gaacagcaca acctcgcgac ggcgcggctg
     1861 tccgtaaaaa atggcccctt ccggaatttc ggtattcagc atttcctcga ggcacatagc
     1921 ctgtgcgcaa agctgcaccc ggtcgcagtc ggacggcttg ggtttccccc gtttatgttc
     1981 caccgggtat gccctgacgg gagatttcaa atttaaaatt tcagatttca gatcagaatg
     2041 aaactcaacc acatccgcaa ttccggaaag gcccagacga agcgacctga gcgggacagc
     2101 atattccatg cgaacgccat cgcgaatttc ggattccctg cggtccaccc ggtcgtgctg
     2161 tgcgcggccc tcggccgtga agcggttttc accccactgc tgctcgatgt gtatcagcgc
     2221 gcattgcctc gggcagtaca ggaagtgctg cagggcagag aggggaagaa ggtctgcctc
     2281 ggaatacatc acgaaccttc cctcttccgc ttccactgtt ccaggcggtc ttcgtgctgt
     2341 ttcttgagat cgtccagcag gttttttgcg ttcttcccct tatgataggt cttcttcgac
     2401 tgttcagtga ggtgtcgcga acccttgatg tccgattcct gcagagaaaa cgcccagccg
     2461 gcgatctgct ttgagatgct gcgcgcctcc gatctcaaat ctgaaatctg agatttcaaa
     2521 tgttcaaaga ccttaagccg gtccatgaca tgacaaatgg atcgaacctc ccctgcagaa
     2581 cctttggcgt agtacaggaa ctggatcaac tccgctgtcg ttcctcgctc gaagccctca
     2641 gcaatgttgt tcgagatgga aagtccggcg cgttgaatct gattggcaat atcgcccctg
     2701 ccctgaaacg cacgatcttc agtcatctca aaaatccgcg cagacaaatc cattgcgtcc
     2761 tgccataccg gcacatcctc gaagttttcg tatttcatca ttacctccat ccgaaggccg
     2821 gcagggttgc tcccggccct cggaatcagg attagatttt tacgtcaact gctacaccgg
     2881 cgggcgcggc ctcgatggtc actttgtagt cagcaaaaga acgtggtgga ttatcaccgc
     2941 atatcttttc aactttgacc agatcaaaca acttctgcgc cggagcgtta ccgagctctg
     3001 attcatgttt gaatacgatg agcttttgcg gagccatttc gccgcgcgcg gcggagcggt
     3061 catggtcaaa catattgatt aaagcatccc agaacaacat caggtcttct tctgaaaacc
     3121 cggtctgctt tgccagcggc gcagagatga agccgtgaac gcggtacaga ccgtagggaa
     3181 tggtaaattt gcggcccatg gtgcgctctt tctctaaatc cgcctctttg gtgacggcca
     3241 tgcgagtaat gctgtgttcc ccagtaaaaa cacgatcaac acttcgggca aacccgaact
     3301 gcaccgggcc acgaacctgt cccgcattca cctcggtact cataactgca ccaaatgtcc
     3361 gtatgtcaaa aaagttctga cacatccatc cagtcacttt ctttgcatcc tcgactcctt
     3421 ttggaagctt cttgctttcc ggcttaagtt cgaatgcctg ataagctcgt tcatgagcac
     3481 ggttcagcac cgccttttct tttacgtaga tttcaaaagg cggctgttcg cctttggtca
     3541 gtccgacgta gttgcggact tttcgcttga ggcaaacatc ggtaacaagg ccgttgccgg
     3601 tttctgggtc gatgcgcgga agatttccgg catcgggatc accgttggga tttccgtctt
     3661 tcacgtcaaa caacaatacg aattcatatc ggttcataat ggggtcactc attgttcttc
     3721 tcctttttct tcggtttcct ttttcgcgaa aaaatcctgc cgctggtggt aatagccgac
     3781 agaaaaacga ccctgatcct ccagcgtcag tgtggtcggc aggccctgtg cgggaatcag
     3841 tcctgttact tcctgcaaca actgctccag ccaaaccgtt ccttttcccg agttgcgaat
     3901 tttggccaga tggtgaatgg ccaaatcctg caaccgctta aatgtgacca gcggcgaact
     3961 ggccgcggca ccgaaatagg tgtcgcgaat tgttttattc aggcctttcc cctgcgcctg
     4021 ctcctgaatg cgctcgagta cggcgaaaag ccgtccgagc acatagccta tgttgtcata
     4081 tgttttgtct aatgccatgg agacctcctt ttgatttgat tttgaaatgc gggcgttgcg
     4141 aacaagaaat gctttgatga tggatgcgcg ggcgaatcca acggactgct ccgccttgca
     4201 acgccggatt gcgttggcaa agagcgtgcg cggatatgcg attcccgtca gaaccgcccg
     4261 tgcgacatcg ccaccgagat tcggcggaat attgtccgcc ttccgttctg tcgccgttga
     4321 aaccagcaac tggaacagtg agaggaattc cggatcatgg ggcgcacgca ccagttccaa
     4381 atcatggaaa tgctgtgcga cgcgttcctt gatttccttc acgttgccgt cgtaccagaa
     4441 gcggattgcg atgcgggagg cgttgggagc gagtccgaga acatagaacg gcagatcagc
     4501 ctcccgtggc ggaatgccgg ttttaactgc cgagagcaat ccgcggattt tgtcatagct
     4561 gaccgactcc tcgccctttg gcggagcaag gaacgaatgg aaatcctgct caaaatcgca
     4621 cggatttttc gcccagaaaa ctgtgctggc atcgcccacc tgcatccgct gattttcgga
     4681 ggcgagtaaa cgattcaaag ccgtcgtata agcaaactga ttgtgctttc caatcggtgc
     4741 atttaaaccc tgatcctttt tggtctttcc atgggagcaa tacgcctcat cgttaaaact
     4801 aacgatatta gcgccagaag tttgagcccc ccagacaccc ttgatcgctg catgcaatac
     4861 agcgacttca tcaatctctc cgctgatagc actggcacat gaacggccgt ttacttcttc
     4921 ataaatcgcc cttatacgtt ccacaacgtt cggacgctgg gcgatcaatc cgacttcatc
     4981 atccaaggca aaggtaatgt tgctgttatg ctccatgatg tcactgagca gggcatgctg
     5041 ccgacaacca tctatccctc ccgtttcata gaaacgaaaa acagcagaga ctccatcatc
     5101 agtcattcca tcaaggtctt tatgcaatct cgccaaaaaa actttgtggt cgtcttttgc
     5161 ctttttgaca tctttcgcct tttcggattt tgggacacca agcacatatt gaggattatc
     5221 ccaaagaaga tttgctttgg atccagaggt cttcttctct cctaacggga cacgaaactc
     5281 tcttgcggat tgctgcttcc ccgtccctgt tcgggtgtcg cgaatattaa ggaaccgccc
     5341 gtctttacca attatgatga caaaagggat tcctttatcc tcaaatccgt atggaggaac
     5401 atccgcagaa tcatcatgaa aaagcctgtc atagtaatct gccaaggctt tcaggatcat
     5461 gagcgcacct ccgcgtttct gaggttgatg gagccgtttt ccatttgagc gtggaagaat
     5521 tgcggcatgg gttcttcacc ggaatagtcc atgtcgtaaa gcatccagcc aaggtctttg
     5581 gttgttccaa tggttggaag cgggtcggcc tccttcggaa tgaattcaaa cgcaaccgga
     5641 aactcgcgac atccaaagta aggccgatgg aaacactgtc cggcggatgc ccggcggata
     5701 aacatctgtt caaacttcgt gaccgaatcc tccgttccgg cctgttccgt catttcaaat
     5761 tccgcgtgaa tgacataggc cacatcgcga aggaatagac cggctcgctg ctgtcgctga
     5821 tcctcaacat aaagccccag atcgccgctt ccgctgttca ttgccgtctg tatatttcgc
     5881 gctgaaatga cggctccgac ctcgttccgc cggacggatt cccacttgat cgggttcagc
     5941 acatcgatct gcaagatatt ccatttgatc gcgggcttcc aaaggatcgc ctccagaatg
     6001 ccgcgtgccg ccgatggcgt catcacctcg tacgaaaccc gttccacttt catctccgga
     6061 cgggtgaaac aggcgttttc gccgctcacc ttcagccgga agggattgct tttgttcatc
     6121 tgttttcctc ctaaatgatc gttgtgtccg gttcgtagcc gtcgagttca tccggcggga
     6181 tgaatccgaa gcggtcggtg taaagtcctt tattgagcag gtaataggtg ccgggatatt
     6241 ccggcagttc gcgggcgtgg ttctcatcca gaagctgcca catcactttt ttaggaacac
     6301 tgacagtgta gcgctggagc ttgcggaaca gcctgcgggc atcccattct atcagcttgt
     6361 ccaggacctc cggggattcg ccgaacggaa caatcagcga aagctgccat ccgtcatcaa
     6421 tcagccggaa ccgttcagag gccgtcctga agctgtattc aagatgtttg cccaacagcc
     6481 cgacgatatt tttctcatcc aacgactgct ctccgagctg gaagaaccgt tgcgcaaagt
     6541 aacgtgaaaa cgtttcgggg aaaaatatct gcgacagccg gtcatcccgc agatgctcca
     6601 tggccagcga tgcgggcgac aggacatagg ccggctgatt ctgcggaaca aagatcacgg
     6661 tttttccaag gtttggaagt tttccctccc ggttgcagcg tccggcagcc tgtgcaatgg
     6721 aatcgagtcc ggccaaggcg cgataaacaa cagggaaatc caaatccacg cccgcttcca
     6781 ccagcgacgt gctgactaca aacagcggat tattccgatc cggaagccgc tgtctgattt
     6841 tttccaaggt ttggaaacga tgctccgcgc acatgttcgt agaaaggtga acggtctgat
     6901 cctcaggaag catcaggctc aacgtgcggg catcctgttt gcggttcaca atgcacaaaa
     6961 ccgattgccc ttcggtctgg atagattccg cgatctgttc atacgcaacc gggtcgagat
     7021 ttgagaatcg ttcgacgtga acccgctgaa gctctccggt cagacggtcg ggccgctcaa
     7081 tgatctcggt tacagactca aaaccctccc tgaattgaaa atcaaaacta tcggttttgg
     7141 tcaacacggg ctgtgtggct gtacagagaa ccggcgttac tccataatat cggtgaagtt
     7201 ccctaattgc cgacaccacc ggtcgcagat actgcggcgg caggcattgc gcttcatcaa
     7261 aaattacgac gctgtcggcg atgttatgca gcttgcggca gcgactggtt ttgctggcat
     7321 agagcgactc aaaaaactga acactggtcg tcacgataat cggcgcatcc cagttttccg
     7381 cagccagccg ttgcctaacc gtttccttcg tctcgtcatc atcaagcaca ttgcagtgat
     7441 gctccagcac ggcgttttca aagccgggaa tattcctgaa aacggatgct gtttgttcga
     7501 tgatgctggt gtacggaatt acgtaaatca cccgtcgttt cccgtgtttc acagcgtggc
     7561 gaagtgcaaa tgccaaggaa gaaagcgttt ttccgcctcc cgtcgggacg gtcaccgaga
     7621 acacagatgg ggcgcaatcc gccatttcac ggcatcgtgc cagaaccgct gcgcggattc
     7681 ggtttacgtc tgtttcatcg gcatcgcggc agagcccgtt cacatgccgg tcgaaggctt
     7741 caagcaaatc gctcagcttc ggatagtccg cctgccgcag ctttttctta tcgctatcca
     7801 tgaatgattc ggtgtcgaga aaatcggcat ccacaacgca ggaaaacagc atgcggatca
     7861 gaaaagaaat atccgcacgc gggttctgta acaacatctt tggcggcggc ggctccggct
     7921 gatctttaac ctcagtcgga acgtttttga gaatatcagt cgattctgag ggattgcgca
     7981 tcttcggttc gagcgccttc gctccggttt cagccgatga gaaatcggcc aacccggcgt
     8041 gatgccccat aatgagccag cagaaaatgc gatccacgcc gccatgcatc acgctttgcg
     8101 ccagatgtcc gcccgcctga gaatgaatca ccgtccccgg tttggtttca aggtgagatt
     8161 caattccatt cgcatcataa agtttcttct ggaactcatt ggaatatttt cccaaatcat
     8221 gccagaggcc ggcgaggcgg gcccattcct ggcctccaaa gggccctgcg aattcggccg
     8281 cgagtgtggc aacgtttttc aaatgttcat cgagcggctg ccatttttcg ggcggctggc
     8341 cctcaagact gtgggcgtag tatttgcgct ccggatcagg catgatggca cctcgggcaa
     8401 ttcatgacgg aagcaagact atggcaaccg ttttctttca gcaagataaa gtttgccgcg
     8461 aaccctgata caaacgatct ggtgtatgcg ggcgtgtttt tctgctttac gcaagtgacg
     8521 ctggcggact tttttacagg ttccgcgaac cccgcaacag tttctgtttt ggaatcagaa
     8581 atactttcgc agccatgcga gggcatcggt cagggagacc tggccggcat agcggacgag
     8641 ggcggcggtc tgcctgacgg cgtccatggg gacgggtttc ggcagcgcgg gggcaaggcg
     8701 gtgatagtgg gcaccaagga gctggcggca ctggtagtcg gcgacgccca tcatgccgtc
     8761 catcatcagg cggataatgg gccgggccca ctgggcatag ccccagtcga ggcgctggcc
     8821 gcggataaac tggcggttca ggccggttcc gaacgaaagc aggcgaatgt gttccattgc
     8881 gggcggccgt gtttttgaac gccggtcgag cgcttgcgtc agcgccgcca tcgagggatt
     8941 gttggcggcc acaccgccgt caatgaagcc ctgatacgtc gggaaatagg acggcgccgc
     9001 acaggagcgc atgatgacgt ccacgatttt ctcccccgca tcagaatcgg gtccggaaaa
     9061 attatggaaa aatttcggct tccagcatcc gtcgccgcgg ccgggcgtcc cgccgaggtc
     9121 gaaggcgggt atgacaactt ttcgcggcag gtcgccgagc gtcgcgcttc cgaaaaggcg
     9181 gatcaggatt tttttgaggc cctgttcgct gaagtcggcg ccgatgaggc gtcccatgtc
     9241 gcgcacattg tctagccggg agtcttcaaa ggcttcgccg ccctgcttcc gataaagttc
     9301 agccagttcc gccggcgacc gccccgctgc aagactgacg gcaagaatgg agcctgtgga
     9361 agtgccggcg aacagcttca catttttcag aaatccgggc tcttctttgt caagccgggc
     9421 cagcagggtg gcggtgtaga ttccgcggat tcccccgcca tcgaacgcgg cgatgcgata
     9481 aagcggactt ttcatcaggg accctctgtt tgggcgaaaa atggcaaaaa aatggcaaaa
     9541 aatcaagaaa aaacggcaaa aatgggccaa aaatggtgat ttttcgcaaa aaaacggaaa
     9601 aaatgccgtg aaaagggaaa ttttcggcgt cgcaggtttc gggtttcaaa ccgttgacac
     9661 cggggcgggg gcatgacagt atgaaccgga attttttcag cggaggatcg ttatggcttt
     9721 tttgattgcc atgtctggag atcacaaggg tgaccggtat gaaatcgaaa ccgatgaggt
     9781 gacgatgggc cggaagccgg actgcaccat tgtgctggac aatccgacca tatcgggaca
     9841 tcactgcagc attctgcgga aggacggact ctatacactt caggacatga attcgaccaa
     9901 cggaacgcgg ctgaattcgc agcgcgtgac cggcacggcc cccctgaagg ccaaggacct
     9961 gattcaggcg ggttcgctgg aattcatgat tgacggcgac gacatccggc tgcccgaaac
    10021 ggagtctttc gccgaggtgg aggtggccga gggcgcggcc gtggcgccgg actccttcgc
    10081 gaatatttcc cccttcggga cgaagcaccg ggaatccaaa ggcctgtcct tcacgattgc
    10141 catgattctg gccgtgattg cggcggcggc gctggcgttc ttcattttcg ccctgatcac
    10201 ggcggtctga cggatattca tggaccgccg gcccagcctg aaacgaactg cggctttcac
    10261 gctgattgaa accatgctgt ccattgcggt cggcgcgctt gtgattctgc tggtattctc
    10321 catctaccac acgacgatgc aggttgttcg cggccaggcc gtgcggcagg agcgttcggg
    10381 ccgggcccat gatgcgctcc gggaaatcag ccgcaggctg gcctgcgcga tggaatatac
    10441 cgcgccggaa acagtttcct tttccgttct tccggcggag gaatccaaaa ccggttttcc
    10501 cgagctcgcc tttatcagtg ccgaggcggc tcccggactc cgccgggaat ggtaccgtcc
    10561 tgttgcggtg aaaatcacct gcgagacaga cggaagaaat tcccgcctgg tcgaaacgct
    10621 gaagcccatg agcggcccgg tgacaacaaa cacgacggtg ctcgccgagg gagcggcggc
    10681 attttccgtt cgagtgtaca atggcgagga atggctcgaa aaatggccgc agccggagca
    10741 gccggttctt ccgcgcgcgg cgcgcatccg cattgagttt cctccggaga ccgggcggga
    10801 tccggcggaa acggaagtgt tcattcctgc cgggaatgcg gtttcccctt ccgagccccg
    10861 ttgaagatca gcttcgcaga acatcctcca gcacggcttc gagctgggga atcacccgtc
    10921 gccaccggaa ccgttcattg accatcgtgc gggcgttatg cgccagccgg gaccggagcg
    10981 cgtgatcctc aagaagaatg tggatctggt tgaacatgat gtccggtgtg tctgcaatga
    11041 ggcagttgtc tcccatctgc gcggggaggc cctcaacgcc cagcgaggtg gatacgaccg
    11101 gcagcccgct ggccatggct tcgaggattt ttccccgcat gccccggccg aggcgaacgg
    11161 gacagacaaa cacctgcgac cggtgcagcc agggtcgaat gtcatccacc cgtccggtga
    11221 ctaccaggcg cttgtcccgt gcggcctgtg cgcggacata gaccggcgga gaggggccga
    11281 ccacataaaa tcgaagcccc ggcagttttt cgcgcagccg cggccagacg gtctgtgtga
    11341 agttgatcac cgcgtcttca ttcgcgggat cgagatagtt gccggtgaag atgatggcct
    11401 cttcgcgcgg agattcctcc ggcggatgat aaaatgaagc gtccaccccg gacggcacga
    11461 cagaaatgcg gagagtcgga tcgagattca gcagggcgta gcgctcctcg cgcgaaagcg
    11521 tgagcacgcg gtcggcgttg cggtagagcc ggcattcgta atcaatcagg cgatacatct
    11581 tgaaccactt cttcagcgcc agaggcccgg ttcccagcag ttcaagctga tgtgccgaca
    11641 taatggcggg gctgtgatgg cacgagatca cgcgccggac ggcattcagc cagcggttcc
    11701 agaagagata ctggcccatc accgtgaact ccgccacgac cacgtcatag cgggaccgct
    11761 cgaccatttc gccaaccagt ctggtcatgg cgatgcttcg cgggtggaca aagggaagcg
    11821 ggacgccgcc gatataggtt tgaacaaatt tacttaaaag gggtggttgc ggcggcatgg
    11881 gctgcagttc cagttcatac aggcggtctt tcagctccgg cacgtgttcg cggccggttt
    11941 cgtcgcagaa cgaggccagg ccgatctcgt gcccgcgcgc accgaggcgg agcatccgct
    12001 catagacaag cgaatggcct ccggcaatgc cggcatacgg aagctgactg gtcagaaaaa
    12061 gtattctcac ggctacactc tatgcgtcgc gcccccgcaa ctcaacagcg aaaaaacccc
    12121 agtttgtctt gacggaccgc ctccgcgggc ctatcaaagt gcggtatttt tgtgatttgt
    12181 ccgggctgac ctgcgttttt cgcgccgggg ggccggattt ggagaacagg agaaacttat
    12241 ggcaaagatt aatttcggcg gagtcatgga aaccgtggtc actgcgaagg agttttccct
    12301 cgcgaaggcc cgcaagattc tgaagaacga agtcgtggcg gtcatcggct acggcgtgca
    12361 gggaccggcc caggcgctca acatgaagga caacggcatc aaggtcattg ttggccagtc
    12421 gaagcagtat ccgaaggact gggagcgcgc gaagaaggac ggatttgtcc ccggcaagac
    12481 gctttttgaa atcgaagaag ccgcaaagcg cggaaccatc atcatgatgc tggtggccga
    12541 cgcggccatg aaaccggtct ggaagattgt caaaaagcac atgaccgccg gcaaggcgct
    12601 ctatttctcg cacggcttcg gccttgtgta caatcacctg acgacggttg ttccgccgaa
    12661 gaacgtggat gtcctgctcg tggcccccaa gggttccgga acatccgtgc gccgcaattt
    12721 cctggccggc gtcggcatca actccagttt cgcggtggaa cagaacgcca ccggccgcgc
    12781 ggtggaccgc tgcaaggcga tcggcatcgc gatcggatcc ggctatctct tcccgaccac
    12841 cttcgagcac gaagtgacca gcgacctcgt cggcgagcgc ggggtgctga tgggcgcgct
    12901 ggcgggcatc atggaggcgc agtatgacgt gcttcgcaag aacggtcaca cgccgagcga
    12961 agccttcaac gagacggttg aagagctcac gcagagcctc atccgcctgg tggatgaaaa
    13021 cgggatggac tggatgtatt ccaactgctc ggcgaccgcc cagcgcggcg cgctcgactg
    13081 gaagccaaag ttcaagaagg cggttcttcc ggtgttcagg gatctgtaca aggccgtgaa
    13141 gaccaagaag gaagccgccc gcgtggtgaa gtcctgcggc aagaagaact ataaagacca
    13201 gctcacaaaa gaactggctg tgatcggcaa ttccgaaatg tggcgcgccg gcgccgcggt
    13261 gcgctcgctg cgcccgaagc gcaaggccgt tgtgatcacc aaggacacca agggtgttgc
    13321 cggccgcaaa ggcaactgat cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnngttgtga tcaccaagga caccaagggt gttgccggcc gcaaaggcaa ctgatcccgg
    13501 cataaaacat caacaggcag gcgggcttcg gcccgcctgc ttcatttttg tcagatatcc
    13561 gccctgttat tttcaattcg gttttgacag agcaaaaccc tccaatgaga aatggaggga
    13621 accgcttcgt cggttccgaa gaatgtcata acagtttggc gatggcgtta gtgctgagct
    13681 gattcagctg attgactatg atgtccgcct catgcagatc ctcggctttg cgggttgtgg
    13741 tcaccgccac gaccttcatt ccggcggcgc gcgcggcctg gatgcccgcg acggcatcct
    13801 cgaacacaac gcaccgagcg ggcggcatcc cgattttgtc ggcggccttc aggaagattt
    13861 cggggtcggg cttgccgtgc ttaacctcat caccagccac catctgcggc agatattcgc
    13921 gaaaacccag cacatcaatc gcgcattcga ggttggcgcg ttcggtggat gatcccacca
    13981 cgcacggaat cccggcatcc tgcaggcggc gaagaaaaac ctccacgccg ggcagtgcgg
    14041 aaagtccttt ctcgcggacc agcctccggt aggtttcctc tttttcgcgc gcaaggcgcc
    14101 ggatctcgac aggatcgctc gcccagccga gaatgttggg aatgatgacc tcgttggtca
    14161 tcccgaagcc cctgacaaaa gcatccggct caaaagtctt ccgttccttc tccgccatga
    14221 tctcccatga cagttgatgg agaggcgcgg aatccacaat caccccgtcc cagtcgaaaa
    14281 tggctcccca ggcggtttcg gacgggcggt gcggggtcga attcatcttt cggcaagctt
    14341 cttgatccat gcgtggtatt tatcgtgaac gccttcgccc agctcctctg attccacgcg
    14401 gtcaaaggcc ttcctgaggc ggtcctggtc gcctgtgttc agcagctgat ccgccatcgg
    14461 atacagcacg tggtcctcct tgttgatatg ctcccgaaga agccgcgcat agagggcaag
    14521 attatcaccg gcctttcgcc gggcctgttc atcgggttcg cgcggggcga cggcattggc
    14581 aatggcgcgc acgcactcgc ggccctgctc gtgctcgtgg agcattaccg cgagcgggcc
    14641 gttttcgaac gggatcccgt gctcatgcat gacggcaaaa agatgttttt cctccttggc
    14701 gtggtggcag cggtccacga agtttcggaa gaaatctgcc atttcccgca ccttctgcag
    14761 gtggattttt ccggtgtcgg caatatacgc agcctcttct tccgccgcgg caacggccag
    14821 cagtatggcc tgatgctcgc gcttcaaaat gtccgttgcc ttcattcgcg gtctccgtat
    14881 ctgtccagcc tgaactatag cccgtctgga gcggttcgtc cacaggccgg cagttatttg
    14941 cgcagcaggc gaagaaggcc gtcgagaatg gtggcggggt ggggcgggca gccgggaatg
    15001 aagagatcaa cgggaatgat ttccgcgacg cccttgtgcg tttcctcctg tccgcggtag
    15061 attcctccgg agacggcgca ggcgccgaca gcgatgacga ttttcggggc gggaacggct
    15121 tcgcaggttt tcttcagagc gagttccatg ttttttgaca caggtcccgt caccagaatt
    15181 ccgtcggcgt gccgcggcga agccacaaac tggatgccga accggccgag gtcccacgcg
    15241 agagtgccga gcacattggt gtccgcttcg caggcgttgc accccccggc gctcacctgc
    15301 cgcagtttca gggatcgccc gaaaagtttg tggatggcct cgctgtaagc attgggggtc
    15361 atgtcgtttc cgggctgtac cacaagatcc tcgcgtttgg ccgaagcaag ccggaagtcg
    15421 tttccaaact tgatggcatc ttttgaacac gccttttcgc acttgccgca gaagatgcat
    15481 aatcctgtat ccagactgat tttcccgtcc gcgacagcaa tcgcgccagt ggggcagact
    15541 ttcgcgcagt ctccgcatcc gttgcagcaa tccccgctga tcaccggccg gccagtgaag
    15601 tgaggcggca tttccggcgc ggggccgtcg ggccatttca tggtccggta tccctgctga
    15661 agcctgactt ttgaaacttt gagcatcgga gtcctctata aatcgtgtcc gcagtaggaa
    15721 aggttgaaac ttttgttgca cagcgggaag tcggaaatct gctgtccgcg cagggccagc
    15781 gccagcccga accagttatg gaatgaggga tccacaacct tgcaggtcag aaaccggcct
    15841 tcattgttcg taatcgcgac atgacagatt tccccgcgcc acccctcaac gagcgcaacc
    15901 gcaagatgtc cggatgcaat agggggattt tcgccggcgc ggactgcgcc gtccggaagt
    15961 gccgccagtt gctccaggat aaacttgtat gaccgctgca gctcctgcca gcgcacgaag
    16021 gcgcgcgcga agacgtcgcc ggattcccac gtggcggccg ggatgtgcga aagcaggaag
    16081 atgccgtatg ggaagtcgct gcggacatcg cgctgcaggc cgcaggcgcg ggcggccaca
    16141 cccacaaggc cgattttaac agcggtttcc cggctcagcg cgcctgtgtt ttcaaatcgc
    16201 gccagcacgg acggcgtttc ccagagcagg ccgatggcag attcagcatc acggaacgcg
    16261 gacttcaggc gcgcggctat tttgtccgca ttttcggaat caatgtcgta gccgactccg
    16321 ccgggaacca cgaggcggcg cccgaagcgg tttccgcaca gcatggctgt aaggttgaga
    16381 aggtcgccgc ggatccgtcc gcagtaggac atggtgggca gatagccaat gtctccggcc
    16441 agcgcgccga ggtcgccgat gtggttggcc atgcgctcca gttcaagggc gatcccgcgg
    16501 atcacctgcg cgcgcgcggg ggcgcggatg cccgccagcg attccacaat gcggctgaag
    16561 gctgtcgcat gcccgacagt ggtgtctcca gcaagcgttt cgatgaagtg aagggatttt
    16621 gaattgggcc cgttcaccag cgatcgctca acgccgcgat gctgataccc tagagaaatt
    16681 tccagatgat ggacggtttc accgtggcac tggaagcgga agtgcccggg ctcgatgatg
    16741 cctgcatgga ccggcccgac tgcaacttcg tgaatttcct cgccttcaat ctgaaagaaa
    16801 ttcgtcacgc caatgccact ggttttttcg gaatcgggaa aacgcacggg cttgagccac
    16861 gggtggccct cgggcatgac atgccactgt tcgaagattt cgcgttcaaa aagatgcgcc
    16921 tgcgggcagt cgggcgtgag ggcgggatat gatttctgca cgggggcgga cagaacggaa
    16981 atagagccgg tgtcgcgcca cgccagaacg gcgatcagcc ggatgttttc accggtggga
    17041 gatccgaaca atgccgaaat ccgcgcacct ttggaaacgc tgtcaagaat atcccgacgg
    17101 aaatcgctca ccgtaaggac gggtatctgt gaaaggggga gcttgcctcc gttgcggatg
    17161 acggccggcc cgctcatgag agacctccca ggacctgtgc ggccctgctc aggattttaa
    17221 gcagaggccc gggaatccac atgcccagca gaagaacaat cgccagcgga atgaaaataa
    17281 gcaacgcctc cggcagcgaa aacttcactg ggccggcctg ctgttccgcc ggcttccacg
    17341 ccattgcaat gatgtgccgc agcacggcta tgaacacgac gccgaggcca agaaggaaga
    17401 gggcggccac gacatacagc gagtgatcaa cagccgcctt caaaacaagg aactcgctca
    17461 ggaaaacaga gaacggggcc acgcccacaa gcgcaagcag gctgatgatg aggcccgcgc
    17521 cccagaccgg cgcgcggcga ataatcatag tcaggcccgg caggtcgtgt gtgccgtaaa
    17581 tctggccgat ccggcccgcg cagaaaaacg cgatgggctt gcagatggaa tggttgatca
    17641 ggtggaacag cgcggcggct gctcccgcgc cgccgattcc ggcgcccagc gccatgatgc
    17701 cgatgtgctc cacgctggaa taggccagca ggcgcttcac atcgcgctgc atcacgatga
    17761 atgcggcggc cacgaggata gagagcagtc cgaaaatcac gagaatgctc cggccccagc
    17821 cctcgtttcc ggttgcggcc tccaccagcg gcaggcaccg caggatgcaa tagagcgcgg
    17881 cgttgagaag gaacccggaa aaaaccgcag acacaggtgc aggtgcctgg ctgtgggcat
    17941 ccgggagcca gttgtgcagc ggcgccagtc cggccttggt gccgtatccg atcacgatga
    18001 atatgaatcc tgccttgagc atcatcgggt tgagtcggga agccgcgtcc atcagagttg
    18061 tccagagcag agcgtccatg ccgtgcagtc cggccggccc tgttgcggcg gccacaagga
    18121 gtattccggt gaatgcgacg gccacaccca cggagcacat cagcagatat ttccacatcg
    18181 cctcgagcgc gcccggagag gcatgaatgc agatcaggaa agcggtcatc agcgtgctgg
    18241 cctccactcc aacccacatc atgccgaggc tgttggaaac cagcaccagc atcatggcgg
    18301 ccagcgatcc gaaccagaga ccgccgtagc gtccggccat cttcggggtg agccgcccgt
    18361 cggcggcctc ttcgcggaag tagatgaggg cgtaaagcga actgaggcag aaaaccgtta
    18421 tcatggcgag cagatgctgc gcggacagcg catcgagcat gagccatccg tttgcggttt
    18481 ccgccggccc gccgcgcagg acgttatacg ccgttatccc cgccagggcc gccgtggcca
    18541 gagtgccggc ggcgcagaat cttaatgcgc cgcgcgcgga ccgcagcagg aggctgatgc
    18601 cgccgaatat cagcggcgcg aggatcagga tggggagtgt attttccgcg ctcatcgtca
    18661 gcccttcaag gcgctcagcc tgtccgcgtc aatgtggtca aactcgcggt tgatgtgata
    18721 aatggcaatg cccatgacga acacggccac gaagatatcc atgagcacgc ccagttcaag
    18781 aaggaggggc atgccgctga cgaccgcaag gccgaagcag tagatgccgt tttcgagcac
    18841 gagatagccg atgacctggc tcatggcggt tctcctggcc acgatcagga aaagcccgca
    18901 ggacatcagt gaaaacgcaa ccgggaccag cagatcggaa ctggaatgca ccgggaggtt
    18961 cagtcttgtg tcgagccaga aggatcccat caggaagaga atgccgaaga agagcgaagc
    19021 catatagccg acaaacggct gcacttcgcg gctggtgtcg gattcgcgca gggcgctgaa
    19081 gagaaggcgg gggaaaacat agcccttgag tccgatcacg gcgacaccga gcagggacac
    19141 ataggaaaaa agatcgccat gctcaagggc cagcgggagc aggccggtca taatgccctg
    19201 cagcgcgctg atgcggatgc ttgcgccaag gcggcttgaa cccaagagca ggaatcctgt
    19261 gaggatcagg gtcaccagca gtgtgtcgag caggatgatc atgacgtgaa cctcagcaca
    19321 agggtgagcg ccagcacgga aatggccacg gcgccggcca gcagctgcgg gaccttcagc
    19381 aggctgagcc gcgccatgat ggattcgaca attcccacaa gcacggaaag gagaatcatg
    19441 cctccagcaa cgccggcaat agccgccggc ccgtgcagcg gcaggaaaga aagaaggacg
    19501 ttcaccagca gcgcgcccag cgcccacatc ttgagggatg aggtgtagag gatgatggca
    19561 aagtcgggcc cggaataatc aagcaccatg acctcgtgaa tcatggtcag ttcaaggtgg
    19621 gtattgggat catccaccgg gatgcgcgca ttttcggcca gaaagacgat gaacagggcc
    19681 gcggaaacca gcgcaagggc cgggccggcg gaaggccccg cggaagctga aaaaatgccg
    19741 gtcagcgaaa gcgatccggt cgcgcgcgcg agggcggcga ggcccagaag cagggcgggt
    19801 tcagccagcg ccgaaaactg gacttcgcgg ctggcgccca tgccttcaaa ggcggaaccg
    19861 gtgtccagcg cggcggccac gaggaaaaag cggagcaggc cgaagc
//
LOCUS       DAOQWR010000033        19858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3092_length_19858_cov_6.435237, whole genome shotgun sequence.
ACCESSION   DAOQWR010000033 DAOQWR010000000
VERSION     DAOQWR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19858
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_19858_cov_6.435237"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..800
                     /locus_tag="PKK36_04210"
     CDS             210..800
                     /locus_tag="PKK36_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNR93797.1"
                     /translation="MTTTELPKFIQQNYEVHEWRHACAILRQDFAAEYKDICDVLTRF
                     RLKKSHIAVGGGRKSKVSEWIDSELSNKGWEEKNFETSITVDDNVMESPTHAVDCFKN
                     RIALEIEWNNKDPFFDRDLNNFRLLFDLRAVSVGIIITRCDELQDIFDSLGRGSSYGA
                     STTHMGKLIPRIQGGGGAGCPLLVFGIRRSLYVEDI"
     gene            802..1458
                     /locus_tag="PKK36_04215"
     CDS             802..1458
                     /locus_tag="PKK36_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNR93798.1"
                     /translation="MPQVSAAEDLTAQVRGPFSTILADPPWRFENRTGKMAPEHQRLL
                     RYPTMTMEEIYELPVNRLAASRAHLYLWVPNALIAEGLEVMKRWGFTYKTNLVWYKIR
                     KDGGPDGRGVGFYFRNVTELVLFGIRGSMRTLEAGRTQTNIISTRKREHSRKPDELYN
                     IIESCSPGPYLELFARHPRKGWAQWGNEDVEDLSSYPIINRTSSQQMVMFDKPARKHG
                     "
     gene            complement(1530..2042)
                     /locus_tag="PKK36_04220"
     CDS             complement(1530..2042)
                     /locus_tag="PKK36_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNR93799.1"
                     /translation="MKNSRIILTGEKQVGKSTVCRKVMDALAPGELFGFRTFRTPEGF
                     ALSSWGGKPAAFASWKSAGRDFEDLQIDLSVFNSAGAAVLVTASPAAWLVMDELGIME
                     QRAGKFVQAVRAAWSGPNPGIAVIQKRALDFWRNELKENPCPVFEIMTENRDAAPGIL
                     LHDLAPESAI"
     gene            2083..2430
                     /locus_tag="PKK36_04225"
     CDS             2083..2430
                     /locus_tag="PKK36_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNR93800.1"
                     /translation="MDVKLKITLVNAAGDEFMGIGLVWLLREIDATRSIHAAAERVGL
                     SYVKALKILNRLEANLPEPVIVRARGGSERGGAELTDFGRKFLKEYDVLQQRIKKSAG
                     SEYASFKRKVKQK"
     gene            2427..4403
                     /locus_tag="PKK36_04230"
     CDS             2427..4403
                     /locus_tag="PKK36_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR93801.1"
                     /translation="MKKIMMVLMLAGAAVGAQEQVFELGDVNITGDRLEQLPVTSQAI
                     VTEPDIRALGAQTADAALRLVPGVYMLNPGKNSRHVNIRGFSESELKVLVDGIPANET
                     FFRTIDLSQIPSAAISGIKVVKGIPSVLYGANSMGGVVNIITKKGGPEPSASAEVQAS
                     DNSTLHLSGSGGSQVSNINIFATAAYQTSDGYFVSDDFDPEDRNTGRNSSYHENGGLR
                     ENSDYDKRAITGKIGYDTEDTKLYLSADWHDNEMGIPTEYNRVWRFTEWTQWHLNLAG
                     EHQAGPVKLLGRAFYFKHDDTLVDDAEATRAVGGKVWFDESVYDDYSAGGSARADWNM
                     LENSVLRAQAGYQFEQSKQRELNTRAFDGAIPNPGWGDEAVYETETWELALENEWKWD
                     SLLAVVGAGYDLYRPLRSTDVDPGDDIDSVNPQAGARWAVTEDIDVYAGAGRKTRFPH
                     MKELYSSHAGGNPDLDPEYTDTVDAGTEISLPFKVPVAISAAAFNNDIRDMILSVNTP
                     EGDTRYENIGHAVTRGLELGTTVVPVENLTLSLNYTLLDAVDEDKDRRLPYLPQHRIN
                     GEARWKIFKGTSLFTQFNYVEDAREYIFDKMTKTESTRELPDYFIWDAGIEQKIFERA
                     TLIARVENILDEDYDIGDGPMPGRAVWAGCRVDW"
     gene            4385..5197
                     /locus_tag="PKK36_04235"
     CDS             4385..5197
                     /locus_tag="PKK36_04235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR93802.1"
                     /translation="MPRGLVMGGPDYIELYRRARAAVPSRSKTPEEWGRRAERMNEGN
                     LLDNSYTRDFLSAVDISGCESVLDVGCGVGNLLIPLCGKLKRGYGLDFSPGMLEAARA
                     NAKKAGLENATIVLRSWEDDWSDIPPADIVIASRCLDVDDMAWALQKLHAHAQRRIYV
                     TYRIGPSYLDEAIYEAIGRKLIPRPDHMLVVNILKQLGMKPKLSTIRTEDKHSAYPDF
                     EAFRKRVEWSLDTLSSDETRKLRDFFENLPRGTDDERVHKHPIEWALISWEK"
     gene            5185..6222
                     /locus_tag="PKK36_04240"
     CDS             5185..6222
                     /locus_tag="PKK36_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93803.1"
                     /translation="MGEIDKSENRQQVITRLTALWGLTEAGLGGVIHALKIPFTGIVV
                     GSTAIILITLIAFFAERRISVLLKSMIVVLIIKASVSPHTPIPAYFAVLFQGLLASFL
                     FRWLPGIRLPAMMLGILALLEGVGQKFIVMTLIYGKSVWEAIDVIGQKLSAQWGLSSK
                     MENASFWLISIFTVYYLIGGVIVGWLAGRIPSRVQRMLEQYREAKPDLKIPDDAIRLP
                     EKARRKWPLRRMLPGLLILLMIVFVFGGMQAGEGSARAKCIYVLVRTTAVLAVWFFLV
                     GPLIRKLIARLQKREAMIYGEEIARAMNLMPRLRQMAALAWAQTAFEKKHVRWYRFLL
                     RLIVYSIVCDD"
     gene            6373..7176
                     /locus_tag="PKK36_04245"
     CDS             6373..7176
                     /locus_tag="PKK36_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNR93804.1"
                     /translation="MTLNSSALEVLKSTSRTFYIPIVRLPGKLRSAVGSAYLCFRAID
                     EIEDHPHLPADEKIRLLNGIAELLREPGENAKGAMKTLFAPSRKALPKVTLNICSYAA
                     MAPAAVRPLISKGTSVMAERMAAWVDRNWSIRTEADLDEYTYDVAGSVGLLLTDLWAW
                     HDGTEAPREDAVGFGRSLQAVNILRNRDEDRRRGVNYFPDGWGFDRMCAYIRKQFERA
                     DAYMEYLSAGPARDFCQIPLTLAKATLDALEGGREKLTREEVLVLTGQS"
     gene            complement(7189..7641)
                     /locus_tag="PKK36_04250"
     CDS             complement(7189..7641)
                     /locus_tag="PKK36_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93805.1"
                     /translation="MTEQLKNDNAEILDTDIVFNCPHCTKSLVIDQSAAGMLVKCTDC
                     GEEIEVPGGAVEEEALHEQAEAPEAIPTVLDSAAEDLAAALAASQQKVQELVAEKQEI
                     NKRRMHLEDERMANLNRFKLISREMNTITAAVGRIMNALRNVESGGKS"
     gene            7779..8357
                     /locus_tag="PKK36_04255"
     CDS             7779..8357
                     /locus_tag="PKK36_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93806.1"
                     /translation="MPDRVNSKFTDNIFVSLQTDTALIRVQGRASFKCSPALKAFCGA
                     AIERGVSRIIMNMETCIGMDSTFMGVLAGVAVRLKEATGGIIQMINLSGRTRGLLATL
                     GLDQMVETWMPGEAPEDLAGHFREDQLDRLSDESPDRREMAQTMLEAHETLVEVFPEN
                     RPKFKDVLTYLREDLKKNGVNPARGDRLSSEH"
     gene            8347..9795
                     /locus_tag="PKK36_04260"
     CDS             8347..9795
                     /locus_tag="PKK36_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNR93807.1"
                     /translation="MNTDAALCLVLAVLFLALAGMLIILFVRNRRLRVLRDAMLQEKE
                     VIYSFVHDVSEVFIGDGSADMDQLLKRVLFYALRTAKAGAGAIYLRGEGNKLEPRAIT
                     GIFPPLSGRPDPGIDRAVSKSQHVEEMVRRHAVSLGEGLIGTVADLGTAELIADAERD
                     VRVPAYAEDFLKIRSLVLVPMRFQQEVMGVLAVVNRVDGAPFIQADLNLLQALADQAS
                     VSVHFFRLREALDAKQRLDRDLEIARKIQSLLLPKHLPRISGFEVAASNLPALEVGGD
                     YYDVIQIDDIHWGIAIADVSGKGISGAIMMAICRSILRSTAVGQTSPAEVLRAVNRLM
                     RSDFAEDMFVSMLYMVLNTQTSRLTLARAGHERPVLFHADAARAAEILDSPGLAVGLG
                     LHEQFDRILQDCSVVLGPGDVLVACTDGITEAMNARQEEWGVDNFLEAVSSSACEGAH
                     SVINNLQQRVVRFIGDAPQYDDMTLIALRKLP"
     gene            9829..10176
                     /locus_tag="PKK36_04265"
     CDS             9829..10176
                     /locus_tag="PKK36_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR93808.1"
                     /translation="MKECVIQREENGQLDILHLDGSLDAYSFPRLETALNQLRENSRN
                     RVVLDCEGLDYISSAALGALIGFARRAREQNGDLKLAKLSAKIYNIVELLGFHKILEI
                     HPEVGQAVSSFAG"
     gene            10189..10641
                     /locus_tag="PKK36_04270"
     CDS             10189..10641
                     /locus_tag="PKK36_04270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR93809.1"
                     /translation="MGLPSEIHSPSGGVVILHTPLHGSYARIIRQAIVDTCVHAGMSE
                     FKTAQMEMAADEASSIIYAARAASSQGAGGQGNLTLRLYQEDKQVVVEICHGGDPIEV
                     GAPADPKTWYGEEDPAAAGLFVIQQFVDELSYRTGTPLGHCLRMIKKI"
     gene            10709..11284
                     /locus_tag="PKK36_04275"
     CDS             10709..11284
                     /locus_tag="PKK36_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNR93810.1"
                     /translation="MSRKKEHTAKKEPAPAAAPPEAPAAPPAPDDAALELTRLKEQLL
                     RLQADFENYRRRVERERTEVYQRANEDLMMELLVILDHFELGLKTAEKHHADKSVLDG
                     FQMVNDQLLAALSRFGLKPVDAEGLEFDPHRHEAVTHVPSEEHPENVVIAQTRRGYLL
                     GDRLLRAAQVVVSSGLPVSAPAPEEGDHGGE"
     gene            11271..12455
                     /gene="dnaJ"
                     /locus_tag="PKK36_04280"
     CDS             11271..12455
                     /gene="dnaJ"
                     /locus_tag="PKK36_04280"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNR93811.1"
                     /translation="MAANKDFYAVLGVDRSAGPDEIKKAYRKLAVKYHPDKNPGDKAA
                     EERFKEISEAYEILSDPAKRQQYDRFGSQAFESGGPGPGGFGFGGIDLEEALRVFMGA
                     TGESGSIFDNFFGGGGRARRNMANRGSDLRFDLEIDFEEAVLGSMRDIEYPVMSECPD
                     CSGSGAQPGSKKHTCRQCGGSGAVAVSSGFFSVRQTCPACSGTGEVIEKVCSACRGSG
                     RVQGRNKLTLKIPPGVETGSRLRAAGKGEGGARGGEAGDLYVVLHVRPHPVFQRQADD
                     IFCEVPIPYDVAALGGEIQVPTIHGYAKLKIPAGTPSGKMFRIRGKGVDLPQQFGKGD
                     HHVRIRIEVPRNLTGSQKKLVRQLGAEVMPDNYPEQRRFYSDADEFFRRKETLETMKQ
                     QG"
     gene            12452..13198
                     /locus_tag="PKK36_04285"
     CDS             12452..13198
                     /locus_tag="PKK36_04285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNR93812.1"
                     /translation="MTVRCHAEPESWTGEEIVLSAAESHHLKHVMRIRPGDEVVVMDG
                     AGREAPAMVAGEHGRALLLKILSVRRHPRPDLAIHLLSAVLKGPKMDWLVQKATELGA
                     ASLLPVQMARCVVRLNERQAADRCERWRQIAQGGIKQCGTPFLPVIGPVTDFAGALTH
                     AGAFDILLICSLGPDARSLRAVLGDAHARGVCSVGVLVGPEGDFTSGEIAAALSADAV
                     PVTLGAQVLRAETASVFALGAIQYEWMSRS"
     gene            13274..14641
                     /locus_tag="PKK36_04290"
     CDS             13274..14641
                     /locus_tag="PKK36_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93813.1"
                     /translation="MKSTVKISIAGLILLCLLPAGASSAQDLDENAALYYWEALALMR
                     QPDSVEEFAVLDFIRNDLPQLPPSILTANTDAAQWLMGDRAMLAALAKAGRCRRCEFF
                     VRSPADPFPALVHLAPMRVLTDRALAVAKAYEFARNSEGAAMIYRDLLLLVRNLDRDI
                     TMRSGLVAADMLQQILFDLEGFLSREPGREAATILSNAFLQSPRPLFHPADYLRMEAV
                     SFRQWMLADPRQMEQKLVRLYGGAAESPAIIRLMALDTPRKVLQMGTWIMDYEAWMLR
                     LANECEAPYERAIGALRWSDAEKVRIEKEASESGGNPLFSLSIPSMSAAYERFLAAEG
                     QFSMAAVLSAAVLYFNDFGRWPVSLDEVSMAAAGPLPRDPFSGQDFFYKTSRLLPRVM
                     LRVPKTMIDEGSILYRMDIRARREQDEKQFQQALKNLEKKWRATAVRAALEVENSPVP
                     GTPRK"
     gene            complement(14654..15721)
                     /locus_tag="PKK36_04295"
     CDS             complement(14654..15721)
                     /locus_tag="PKK36_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93814.1"
                     /translation="MENGNTSDPPQKRDAASREAEAIQPRRHRRSFRNASRPRRSYTS
                     RTPATFWGVMISLLTAYLILLFAAQCHRRALHRERVQAVLMPQAKPAPVQSTERAGLP
                     DDIEEQVAKLIALAEESAGVLDAAAQQAEGGDRALAAERLRAQLAAAPDNVSLKLGLA
                     RLLLDEGNRSEAITILVECLRTSPGQHETRLLLAESLLAEKAYEPAAQMAEWILDEDN
                     YNTRAHDIAASAYMLNGSAAAAIPHLRRMATLERDDTAIQNRLAQAHMRAGEFSKAQE
                     IFQDILEIDPDNSVTYYNLAACYALQNLPEQSAQVLTNALRKFDSTFVASWLQGADFD
                     TVRTNESFTVLLTGLPETNGF"
     gene            complement(15733..16047)
                     /locus_tag="PKK36_04300"
     CDS             complement(15733..16047)
                     /locus_tag="PKK36_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNR93815.1"
                     /translation="MRNIIKWIPAALALVLMLGSAGCTTVVYDMGEGYTDRDASLERD
                     VINRLREDDLTGKYLFGVSAEGATVTLRGSVPNEMVRSRAIGIAYGATGVQNVIDQLR
                     SW"
     gene            complement(16118..16495)
                     /locus_tag="PKK36_04305"
     CDS             complement(16118..16495)
                     /locus_tag="PKK36_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93816.1"
                     /translation="MRMLLTCWTAALLLGAGCAGTAPMRPPAAATPVLAGTPQIRWVP
                     GATVVHVDQKNGHAVLECLIIPEAGTEAEVFHEGQKCAVLLMTGQRNGSFAAGRIVSG
                     RPETGDLVQFMRVVYPRRPSPGE"
     gene            complement(16492..17145)
                     /locus_tag="PKK36_04310"
     CDS             complement(16492..17145)
                     /locus_tag="PKK36_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNR93817.1"
                     /translation="MKNSKLPAKAVYRALFKRWGPQHWWPGETPLEVAVGAVLTQNTN
                     WRNVEKAIANLKKSGALNVRSLSQMPAKTLAGRIRPAGYFNMKARRLKAFIETVRKTS
                     GFTMKKMEQIETGTLRGILLNTNGIGPETADSILLYAFGRPVFVVDAYTRRMLERHRW
                     TDEGAAYDDIAALFTESCPRDSSLYGEYHALIVKLGKEHCRKRALCEGCPLEQWLPS"
     gene            17225..18154
                     /gene="era"
                     /locus_tag="PKK36_04315"
     CDS             17225..18154
                     /gene="era"
                     /locus_tag="PKK36_04315"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNR93818.1"
                     /translation="MNADQNKPMNPAGRSGIVAVVGRANTGKSTLVNCILEEKISIVS
                     PVAQTTRNVIRGVLTEPRGQIVFLDTPGIHKAESELGKIMNRSARASTEGTDIILMVF
                     DASRPPRDEDEGWLRRALFEEIPVAAVLNKADQPEDCSSRYMDLWRELEGEKRVQKEI
                     PWLRTSALENSGVRELVDFLFEQLPEGPHLFPDDVLTDFPRKLAVADIIREKLFHRLR
                     EELPHAVAVVVDTLDEAADGTWGIKCTIYVNRHSQKAIVIGEKGRLLRSVRLASQKEI
                     RSVYDHPVHLDLWVKVEPNWSKNFWLLKKLGYQ"
     gene            18151..19758
                     /locus_tag="PKK36_04320"
     CDS             18151..19758
                     /locus_tag="PKK36_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93819.1"
                     /translation="MKSGRRELWMVLALLAVAPALLYLPGFGHDFVYDDHGQLEENPF
                     LQNPENVREVLMLGTLGDARVINGRRPFVLLTYFSDRAVWGARAAGYRLTNFAAHAGS
                     VWLFFFLLLRLSGRRAFSFSAALLFSLHPVLIEAVHVPAFRPDVFCGLFSFAFLLAAV
                     RTEMKTARQTALAAVLLWLALCSKEAAAVLPAVLAGVWICLPQTRLPVRRMALLLAVS
                     LGLVLLFMKLSSGSGLQAVGGAWNGRSLQPPHNFFTSPWLFLRMVGLLAVPWPLVADR
                     VVLPVTSTADLRFAAGLLAVAVCLCAAIFFLRRKPWISLSLGWMLAAYLPVSSLVPLF
                     NPLAERYLYFMAAGFCILPAHLLTSDGGSRSAATVSMGVIASKALLGFICVLYLLIGW
                     IRMPAWKNDVALWTRTLADEPNSMRARVWLALDLKEQGRREEALTLLDEAIRINPQDV
                     SGFINRGILWGEMGYYDRAEADLAEAVRRRPESSSAHWNLAVMLQHKGDIGGMLRELE
                     KTLELNPEHEKALQVKKSLESAVPENR"
BASE COUNT         3912 a         5655 c         6107 g         4184 t
ORIGIN      
        1 acctacttca tcaacacgaa aggcatgaaa ggcggcacgg gtggtggcct cagtgcccac
       61 cgaaccgtat acgtgtacat cttctagccg gaagaacaaa gacctccgaa ccatagaggc
      121 catcggcggc ctgcggccat ctcaacctca tggctgatgt cgacgaaaga aatgttgaaa
      181 aatgacatgc agcgtgcaac agtaacaata tgactacaac ggaattgccc aaattcatac
      241 agcagaatta cgaggtccac gagtggcgtc atgcatgcgc cattttgcgg caggattttg
      301 cggccgaata taaggatatt tgtgatgtct tgactcgttt caggctcaag aaaagtcata
      361 ttgccgtcgg cggtggacgc aaatctaaag tttccgaatg gattgattcc gagctgtcga
      421 ataaggggtg ggaggagaag aatttcgaaa cgagcataac tgttgacgac aatgtgatgg
      481 agtcgccgac acatgcggtt gactgtttta agaatagaat cgcgctggaa attgagtgga
      541 ataataagga cccttttttc gacagggatc tgaataattt ccgcctcctt tttgatcttc
      601 gcgctgtatc tgtgggaata attattacgc gctgtgacga attacaggat atatttgatt
      661 ctctaggtcg cggatcatct tatggcgcat ctacaaccca tatgggaaaa ctcattcccc
      721 gcattcaggg tggtggtggc gcaggatgcc cgctgttggt ttttggaatt cggagatcac
      781 tctatgtcga agatatttaa tatgccccaa gtttccgctg ccgaagatct taccgcccaa
      841 gtcaggggcc ctttttcgac aatccttgcc gatccccctt ggcgttttga aaatcgcacc
      901 ggcaaaatgg ctcccgaaca tcaaagactt ttgcgatatc cgaccatgac aatggaagag
      961 atttatgaac tgccagttaa tcgccttgcc gcatcgcgag cgcatcttta tctctgggtt
     1021 ccaaatgccc tcatcgcaga aggccttgag gtaatgaagc gttgggggtt cacctataaa
     1081 acaaatctag tttggtacaa aatcagaaaa gatggtggtc ccgatggccg gggtgtgggt
     1141 ttctacttcc gtaatgtaac tgagttagtc cttttcggga ttcggggcag tatgcgcaca
     1201 cttgaagcag gacgcaccca aacaaacatc atatccacca gaaaacgcga gcattcgcga
     1261 aagccagacg aactctacaa cattattgag tcctgttctc ccggcccata tcttgaattg
     1321 tttgcgcgcc accccagaaa gggttgggct caatggggaa atgaagacgt tgaagatctt
     1381 tcctcatatc ccattatcaa ccgcacctcg tcacagcaaa tggtgatgtt tgataaaccc
     1441 gcacgaaaac acggataaaa tatgttgaac gggctggggt ctaccggcgc cccgcctccg
     1501 gctcaagcac cggagactca ctcctgaagt tatatcgcgg attccggcgc gagatcgtgc
     1561 aagaggattc cgggcgcggc gtcgcggttt tccgtcatga tctcgaaaac ggggcagggg
     1621 ttttctttca gctcattccg ccagaaatcc agcgcccttt tctgaatcac agcgattccg
     1681 ggattcggac cggaccatgc ggcgcggacg gcctgcacaa actttccggc gcgctgctcc
     1741 atgatcccga gctcgtccat aaccagccat gccgcgggtg acgcggtaac caggaccgcc
     1801 gcgccggcgg aattgaaaac ggacaggtca atctgcaggt cttcgaaatc gcggcccgcc
     1861 gatttccagg atgcgaaggc cgcgggtttc ccgccccatg aggaaagcgc aaagccttcc
     1921 ggcgtccgga acgtgcggaa gccgaagagt tcccccggcg ccagcgcatc catgaccttc
     1981 cggcagaccg tgcttttgcc gacctgcttt tctcccgtta gaataatcct gctatttttc
     2041 atttgccttc cgatataaac gcctatatat atcggcacgc ttatggatgt aaaactaaaa
     2101 ataacgctgg tgaacgcggc cggcgacgaa tttatgggca tcggactcgt gtggctgctc
     2161 cgcgaaatag acgccacgcg ctcgatccat gccgcggcgg agcgcgtcgg cctctcctat
     2221 gtcaaggcgc tcaagattct taaccggctg gaggccaatc tgccggagcc cgtgattgtg
     2281 cgcgcccgcg gcggcagtga aagaggcggc gcggaactga cggatttcgg caggaaattt
     2341 ctaaaggaat acgacgtgct ccagcagcgc ataaaaaaat cggcgggcag cgagtacgca
     2401 tcattcaaaa gaaaggtgaa acaaaaatga agaagatcat gatggtttta atgctggccg
     2461 gcgcggctgt gggcgcccag gagcaggtat ttgaactggg cgatgtgaat atcaccggag
     2521 accggctgga gcagcttccc gtgacctcgc aagccattgt gacggagccc gacatccgcg
     2581 cgctgggcgc gcagacggcc gatgcggcac tgcggctggt tccgggtgtg tacatgctga
     2641 accccggcaa gaattcccgg cacgtgaaca tccgcggatt ttcggaaagc gaactgaagg
     2701 tgctggtgga cggcattccc gccaacgaga ccttcttccg caccattgac ctttcgcaga
     2761 ttccatccgc cgctatttcc ggcatcaagg tggtcaaggg catcccgtct gttctgtacg
     2821 gcgccaacag catgggcggc gtggtcaaca tcatcaccaa gaagggcggc ccggagcctt
     2881 ccgcatccgc cgaggtgcag gcctcggaca actccaccct gcacctgtcc ggaagcggag
     2941 ggagccaggt cagcaacatc aacatatttg caacggcggc gtatcagacg agcgacggct
     3001 atttcgtgtc cgatgatttt gatccggaag accggaacac gggccgaaac tcttcgtatc
     3061 acgagaacgg cggactgcgc gagaacagcg attatgacaa gcgggcaatc accgggaaaa
     3121 tcggctatga cacggaggat accaagctct atctgagcgc ggactggcac gacaatgaaa
     3181 tgggcattcc gacggaatac aaccgcgtgt ggcgcttcac cgagtggacg cagtggcacc
     3241 tgaacctcgc gggcgagcat caggccggcc cggtgaagct gttgggccgc gcgttttatt
     3301 tcaagcacga cgacacgctg gtggatgatg cggaggccac aagggccgtg ggcgggaagg
     3361 tgtggtttga tgaaagcgtt tatgatgact acagcgccgg cggttcggcc cgcgccgact
     3421 ggaacatgct ggaaaacagc gtgctccgcg cgcaggccgg ctatcagttt gaacaaagca
     3481 aacagcggga gcttaacacg cgagccttcg atggcgcgat tcccaatccc ggctggggcg
     3541 atgaagcggt ttatgaaacc gaaacatggg agcttgcgct cgaaaacgaa tggaaatggg
     3601 actcgcttct ggctgtggtc ggcgcgggct atgacctgta ccgcccgctc cgttcgacgg
     3661 acgtggatcc cggcgacgac attgattccg tcaatccgca ggccggcgcg cgctgggcgg
     3721 tcaccgaaga tatcgatgtg tacgcgggcg cgggtcgcaa aacgcgcttc ccgcacatga
     3781 aggagctcta ttcttcgcac gccggcggca atccggatct cgatccggaa tacacggaca
     3841 ccgttgacgc cggaacggaa atcagtttgc cctttaaagt gcctgttgcg atcagcgccg
     3901 cggccttcaa caacgatatc cgcgacatga tcctctcggt caacactccg gagggcgaca
     3961 cgcgctacga gaacatcggc catgccgtga cccgcggact ggaactcgga accacggtcg
     4021 tgcccgttga aaatctcacg ctgagcctta actacaccct gctggacgcg gtggacgaag
     4081 acaaggaccg ccgccttccg tatctgccgc agcaccgcat caacggcgag gcgcgctgga
     4141 aaatattcaa gggaacatcg cttttcacgc agttcaacta tgtcgaggat gcgcgggaat
     4201 atatcttcga caagatgacc aaaaccgaat ccacgcgcga gcttccggat tacttcatct
     4261 gggatgccgg cattgagcag aagatctttg agcgggccac gctgattgcc cgtgtcgaaa
     4321 acattctcga cgaggactac gacatcggcg acggcccgat gcccggacgc gccgtttggg
     4381 ccggatgccg cgtggactgg tgatgggcgg tcccgattac attgaactgt accggcgcgc
     4441 gcgcgcggcc gttccttcgc gttccaagac cccggaggaa tggggccgcc gcgccgaacg
     4501 catgaatgag ggcaaccttc tcgacaactc gtatacccgc gattttctca gcgctgtgga
     4561 catttccggc tgcgaaagtg tgctggatgt cggatgcggg gtcggcaatc tgctgattcc
     4621 gctgtgcgga aaactgaaac ggggctatgg cctcgatttt tcgcccggca tgctggaggc
     4681 cgcacgggcc aatgcgaaaa aggccggcct tgaaaacgcc acgattgttt tgcggtcatg
     4741 ggaggatgac tggagcgaca ttcctcccgc cgacattgta attgcctcgc gctgtcttga
     4801 tgtggatgac atggcgtggg cgctgcaaaa actgcatgcg cacgcccagc gccggattta
     4861 cgtcacctac cgcatcgggc cgagttatct cgacgaggcg atctatgaag ccatcggccg
     4921 caagctgatt cctcggcccg atcacatgct ggtggtgaac attctgaaac agctgggcat
     4981 gaagccgaaa ctgtccacta tccggaccga ggacaagcac tctgcatatc cggatttcga
     5041 ggcgttccgc aaacgcgtgg agtggagcct tgacacactg tcctccgacg agacgcggaa
     5101 actgcgtgat ttttttgaaa accttccgcg cggcacggat gatgagcgcg tgcataaaca
     5161 tcctatcgag tgggccctga tttcatggga gaaatagaca aatccgaaaa ccgccagcag
     5221 gtcatcacgc ggctgaccgc gctttggggg ctgactgaag ccggactcgg cggcgtgatc
     5281 cacgcgctga aaatcccgtt cacgggcatt gtggtcggct cgacggccat cattttgatc
     5341 acgctgattg cgttcttcgc ggaacggcgg atatctgtcc tcctcaaatc aatgatcgtg
     5401 gtgctgatca tcaaggcgag cgtcagcccg cacaccccca ttcccgcata ctttgccgtg
     5461 ctgtttcagg gcctgctcgc gtcgtttttg ttccgctggc tgccgggcat ccggcttccc
     5521 gccatgatgc tcggcattct tgccttgctg gagggggtcg gccagaagtt tatcgtgatg
     5581 acgctgattt acggaaaatc ggtttgggaa gccatcgacg tgatcgggca gaagctgagc
     5641 gctcaatggg gactgagctc caaaatggaa aacgcgagtt tctggctgat ttcaatattc
     5701 accgtttatt atctgattgg cggcgtgatt gtcggatggc ttgcgggccg cattccttcg
     5761 cgtgtgcaga ggatgctcga acagtaccgc gaggcgaagc ccgacctgaa aataccggac
     5821 gacgccatcc ggctgccgga gaaggcccgc cgcaagtggc cgctgcgccg gatgcttccg
     5881 gggctgctga tcctgctgat gatcgtattc gtgttcggcg gcatgcaggc cggggaggga
     5941 tcggcccgtg cgaagtgtat ttatgttctt gtccgcacaa cggcggttct ggcggtctgg
     6001 ttttttctgg tcgggccgct gatccgcaag ctgattgcgc ggctccaaaa gcgcgaggcg
     6061 atgatttatg gcgaggaaat cgcccgggcc atgaacctca tgcccagact gcgccagatg
     6121 gccgcgctgg cctgggccca gaccgctttc gagaaaaagc acgtgcgctg gtaccggttt
     6181 ctcctgcgcc tgattgtcta cagcattgtc tgcgatgatt aacgggcgcc ggcggcgcgg
     6241 agttccgccc tccagttcgg tgcgtcaaac gccaatatcc tcattcgggg ggtcgcgcgc
     6301 tgtcgcgacc ggtgcgagga ttgattcttc cgatggttgc gcatctcccg gttctcggct
     6361 atctttccgc ccatgacatt gaacagctca gcgctggaag ttttaaaaag caccagcagg
     6421 actttctata ttccgattgt gcggctgccc ggaaaactgc gcagcgcggt gggatcggcc
     6481 tatctctgct tccgcgccat tgacgaaatc gaggatcatc cccatctgcc tgcggacgaa
     6541 aaaatccgcc ttctgaacgg gattgcggaa ctcctgcgcg agcccggaga aaatgcgaag
     6601 ggcgccatga aaactctttt tgccccctcg cgcaaggcgc ttcccaaagt cacgctgaac
     6661 atctgttcct atgccgccat ggcccccgcg gctgttcggc cgctgatttc gaagggcact
     6721 tccgtcatgg ctgaacggat ggcggcctgg gttgaccgca actggagcat ccgcacggaa
     6781 gcagacttgg atgaatacac gtatgacgtg gccggaagcg tggggctgct tctgactgat
     6841 ctttgggcct ggcatgacgg caccgaggcg ccgcgcgaag atgccgttgg cttcggccgc
     6901 agtctgcagg cggtgaacat tcttcgcaac cgcgatgagg atcgccgccg cggagtcaat
     6961 tatttccccg acggatgggg cttcgaccgc atgtgcgcct acatccgaaa gcagttcgag
     7021 cgggccgacg catacatgga atatctgtca gccggtccgg cgcgcgattt ctgccagatt
     7081 ccgctgacgc tggccaaagc cacgctggat gccctggagg gcggccgcga gaagctcacg
     7141 cgcgaagaag tgctcgtgct gaccggacaa tcctgaccgc gcgcggtttc aggatttccc
     7201 gccggactcc acgtttctca gggcgttcat gatgcggccc acggccgcgg tgatggtgtt
     7261 catttccctc gaaatgagct tgaaacggtt gagattggcc atgcgctcgt cctcaaggtg
     7321 catccggcgc ttgttgattt cctgtttttc cgccaccagc tcctggactt tctgctgcga
     7381 tgcggcgagg gcggcggcca ggtcttcggc ggcagaatcc agcaccgtgg ggatggcttc
     7441 gggggcttcc gcctgctcgt gaagtgcctc ctcctccaca gcgcctcccg gaacctcgat
     7501 ttcctctccg cagtcggtgc atttcaccag catgccggcc gccgactggt cgatcaccag
     7561 gcttttggtg caatgggggc aattaaagac aatatcggta tccagaattt ccgcgttgtc
     7621 gtttttcagc tgctcagtca ttggaccacc tcgtattttc tgagagactg ccacattctc
     7681 gagaatacat caagggagaa gcgcgccttt tcggccgccg cgattgacag gcatttctcc
     7741 gggtgttagg ttcttcggat attttgggaa tggtcgaaat gcccgacaga gttaacagca
     7801 agttcacgga taacattttt gtgagccttc agaccgatac cgcgctcatt cgggtgcagg
     7861 ggcgcgcctc gttcaagtgc agtcccgcgc tgaaggcgtt ttgcggtgcg gccattgagc
     7921 ggggagtctc ccggatcatc atgaacatgg aaacctgcat cggcatggac agcaccttta
     7981 tgggggttct tgcgggtgtg gcggtgaggc tgaaggaggc gacgggcggg atcattcaaa
     8041 tgatcaacct gtcgggccgc acacgcggac tgctcgccac actgggactg gaccagatgg
     8101 tggagacctg gatgccgggc gaggcgcccg aggatctggc cgggcatttc cgggaagacc
     8161 agcttgaccg gctcagcgat gaaagtccgg accggcggga aatggcccag accatgctgg
     8221 aggcgcacga gacgctggtt gaagtttttc ccgagaacag gcccaagttc aaggatgtgc
     8281 tcacctatct gcgtgaggac ttgaagaaaa acggcgtgaa tccggcccgg ggagatcgtc
     8341 tttcaagtga acactgacgc cgcattgtgt ctggttctgg ctgtgctttt tctggcactg
     8401 gccggcatgc tgattattct gtttgtgcgc aaccgccgcc tgcgtgttct gcgtgacgcg
     8461 atgctgcagg aaaaagaggt gatctacagt ttcgtgcatg atgtcagcga ggtgttcatc
     8521 ggggacggct cggcggacat ggatcagctg ctcaagcggg ttctcttcta tgccctgcga
     8581 acggcgaagg ccggggccgg tgcgatttat ctgcggggcg agggaaacaa gctggagccg
     8641 cgcgccatta ccggcatctt cccccctctt tccgggaggc ctgatccggg catagaccgc
     8701 gcggtcagca agtcgcagca tgtcgaggaa atggtgcgcc ggcatgcggt gtcgctgggc
     8761 gagggactga tcggaactgt cgcggatctt ggaacggccg agctgattgc cgatgccgag
     8821 cgggatgtgc gggtcccggc ctatgcggag gattttctca aaatccgctc gcttgtgctg
     8881 gttccgatgc gttttcagca ggaagtgatg ggtgtgctgg cggtggtcaa tcgcgtggat
     8941 ggcgcccctt ttattcaggc ggacctgaac ctgctgcagg cgctggccga tcaggcctcg
     9001 gtgtcggttc attttttccg tttgcgcgag gcccttgacg cgaaacagcg gctggaccgc
     9061 gatcttgaga ttgcccgcaa gattcagtct ctgctgcttc cgaaacatct gccgagaatc
     9121 agcgggtttg aggtggcggc ctccaatctc ccggcgctgg aggtcggcgg agactattat
     9181 gatgtgatcc agattgatga cattcactgg ggcatagcga ttgccgatgt gtccggcaag
     9241 gggatcagcg gagccattat gatggcgatc tgccggagca ttctgcgttc gacggcggtt
     9301 gggcagacca gccccgcgga agtgcttcgg gcggtcaacc gcctgatgcg ctcagacttc
     9361 gcggaagaca tgtttgtgag catgctctat atggttctca acactcaaac cagccggctg
     9421 actcttgcgc gcgcaggtca tgagcggccg gttctgtttc atgcggatgc cgcccgcgcc
     9481 gccgagattc tggattcgcc gggtctggcc gtgggccttg gcctgcacga gcagtttgac
     9541 cggattctgc aggactgcag cgttgtcctt ggccccggcg atgtgctcgt ggcctgcacc
     9601 gacggcatta cggaggccat gaacgcccgg caggaggagt ggggcgtgga taacttcctt
     9661 gaagcggtca gcagttccgc ctgcgaaggg gcccacagcg tgatcaacaa tctgcagcag
     9721 agggtggttc gcttcatcgg cgatgcccca caatacgatg atatgacctt gattgctctg
     9781 cggaaactgc cataatatgc ggcatggttt acagatggag atgaatctat gaaggaatgt
     9841 gtaatacagc gcgaagagaa cggacagctg gatatcctgc atctggacgg ctcactggat
     9901 gcttattcat ttccccgtct tgagacggcc ctcaatcagc ttcgcgaaaa cagccggaac
     9961 cgcgtcgtgc tcgattgcga aggccttgac tacatcagct ccgccgcgct gggggccctg
    10021 atcggatttg cccgccgcgc ccgcgagcag aacggcgacc tgaaactcgc gaagctttct
    10081 gcgaagattt acaacatcgt ggagctgctt ggatttcaca agatccttga aatccatccg
    10141 gaagtggggc aggcggtcag cagttttgcc ggttaggggg acgcgggaat gggcctcccg
    10201 tcagaaatcc attctccttc cggaggagtg gtgattcttc atacgcccct gcatggcagt
    10261 tatgcgcgaa tcattcggca ggccattgtg gacacctgcg ttcatgcggg aatgagcgaa
    10321 ttcaagaccg cccagatgga gatggcggcc gatgaggcgt catccatcat ttatgcggca
    10381 cgggcagcct cgtcacaggg cgccggcggg caggggaatc tgacccttcg gctgtatcag
    10441 gaggacaagc aggtcgttgt ggagatttgt catggcggag atcccatcga ggtgggtgcc
    10501 ccggcagatc cgaagacatg gtacggggag gaagatcccg ccgccgcggg actctttgtg
    10561 attcagcagt tcgtggacga actgtcctac aggaccggaa cgcctctggg ccactgcctt
    10621 cggatgataa agaagatata gtgcgcttcc gctgcaggcc gtgtccgcag aataaataca
    10681 ccgtttatcg catagagagg ttttcagcat gagcagaaaa aaagaacaca ccgccaaaaa
    10741 ggagccggcg ccggccgcag ccccgccgga ggcgcctgcg gcccccccgg cccccgatga
    10801 cgccgcgctt gaactgacgc gactgaagga gcagctgctc cgccttcagg cggatttcga
    10861 aaactaccgc cgccgggtgg agcgggagcg caccgaagtc taccagcgcg ccaacgaaga
    10921 tctgatgatg gagcttctcg ttattctgga ccatttcgag ctgggtctga aaaccgccga
    10981 aaagcatcac gccgacaagt ccgtgctgga cggattccaa atggtgaacg atcagctgct
    11041 ggcggcgctt tcccgtttcg ggctgaagcc ggtggacgcg gaagggctgg aatttgatcc
    11101 gcaccggcac gaggccgtta cccacgtgcc gtccgaagag catcctgaaa acgtggtgat
    11161 tgcacaaacg cgtcgcggct acttgctggg cgaccggctg ctccgcgccg cccaggtggt
    11221 ggtgtcaagc ggtctgccgg tgtctgctcc cgcgccggag gagggtgacc atggcggcga
    11281 ataaggattt ttacgccgtg ctgggggtgg accggagcgc cgggccggat gagattaaga
    11341 aagcttaccg gaaacttgcg gttaaatatc atcccgacaa gaatccgggc gacaaggcgg
    11401 cagaggagag gttcaaggag atttcggaag cctatgaaat tctcagcgat ccggccaagc
    11461 gtcagcagta cgaccggttt ggttcgcagg cttttgagag cggcgggccc ggccccggcg
    11521 gcttcggctt cgggggcata gatctcgagg aggcgttgcg cgtcttcatg ggcgccaccg
    11581 gagaatccgg aagcattttc gacaatttct tcggcggcgg tggccgcgcg cgccgcaata
    11641 tggcgaaccg cggcagcgac ctgcggtttg accttgagat cgattttgaa gaagcagttc
    11701 tgggttccat gcgcgatatc gaatatcccg tcatgagcga gtgtccggac tgctccggaa
    11761 gcggcgccca gccgggctcg aaaaaacaca cctgccgtca gtgcggaggc tccggcgccg
    11821 tggccgtgtc cagcggtttt ttcagtgtgc ggcaaacctg ccccgcctgc agcggcacgg
    11881 gcgaagtgat tgaaaaggtc tgttccgcct gtcgcgggtc cggccgcgtg cagggtcgca
    11941 acaagctgac gctgaagatc ccgcccggcg ttgaaaccgg ctcgcgtttg cgcgcggccg
    12001 gaaagggcga gggcggggcc cgtggcggag aggcaggcga tttgtatgtg gttcttcacg
    12061 tgcgcccgca tcccgtgttc cagagacagg ccgatgacat attctgcgag gtgcccattc
    12121 cctatgatgt ggctgcgctc ggcggggaga tccaggtgcc caccatccac ggatatgcca
    12181 aactcaaaat cccggccggc acgcccagcg ggaagatgtt ccgcatacgc gggaaggggg
    12241 ttgatcttcc gcagcagttc ggcaaggggg atcatcatgt gcgcattcgc attgaggtgc
    12301 cccggaatct gactggaagt cagaaaaaac tggtgcgcca gctgggtgcg gaagtcatgc
    12361 cggacaacta tcccgagcag cgccggttct attccgatgc cgacgaattt ttccgccgca
    12421 aggaaaccct cgaaaccatg aagcagcagg gatgaccgtt cgctgtcatg ctgaaccgga
    12481 gagctggacg ggcgaagaaa tcgttctttc ggctgcggag tcccaccacc tcaagcatgt
    12541 catgcggatt cgtccgggcg acgaggtggt tgtgatggat ggggcggggc gggaggcgcc
    12601 ggccatggtg gccggggagc acggccgtgc gcttcttctg aaaatcctgt cggtccggcg
    12661 gcatccccgt ccggaccttg cgattcatct tctgagtgcg gttctgaagg ggcctaaaat
    12721 ggactggctg gttcagaaag caacagaact gggcgccgcc tcgctccttc ccgttcagat
    12781 ggctcgctgt gtggtgcgcc tgaatgagcg gcaggcggcg gatcgctgcg agcggtggcg
    12841 ccagattgcg caggggggca taaagcagtg cgggacgccg tttctcccgg tcattggccc
    12901 ggtaacggat ttcgccggag cgctgacgca cgccggcgcc ttcgacatcc tgctgatctg
    12961 ctcgctgggt cccgacgccc gttccctgcg ggccgttctc ggcgacgctc atgcgcgagg
    13021 cgtctgctcc gtcggagtgc tggtgggtcc ggagggggac ttcacgtccg gggaaatcgc
    13081 cgcggcgctc tcagcggacg cggttccggt tacgctgggc gctcaggtgc tgcgcgcgga
    13141 aacagcgtca gttttcgcgc tgggcgcaat tcagtatgaa tggatgagcc gcagctgact
    13201 ctgtattcgc aaacagttgc actgtcatgc gtttcgggat agacttctgc ccgttacgga
    13261 gatgtgcgga attatgaaaa gcactgtaaa aatttccatc gcggggctga ttctgctgtg
    13321 tctgcttccg gccggcgcgt cgtcggctca ggatctggat gaaaacgcgg cgctctacta
    13381 ctgggaagct cttgcgctga tgcgccagcc ggacagtgtg gaagaatttg ccgttcttga
    13441 tttcatacgg aacgatcttc cgcagcttcc gccttccatt ttgaccgcga acacggacgc
    13501 cgcccagtgg ctcatgggcg accgcgccat gctggcggcc ctcgcgaagg cgggccgctg
    13561 ccgccggtgc gagttctttg tgcggtctcc ggctgatcct ttcccggcgc tggttcatct
    13621 ggcccccatg agggtgctga cggatcgagc gctggcggtt gccaaagcct atgaattcgc
    13681 ccgcaacagc gaaggcgccg cgatgattta ccgcgacctg cttctgctgg tgcggaacct
    13741 ggaccgggac atcaccatgc ggtccgggct ggtggctgct gacatgctgc agcagattct
    13801 ttttgatctg gagggattcc tgtcgcgcga gccgggccgg gaggccgcga cgatcctgtc
    13861 gaacgctttt ctgcagtcgc cccggcccct ttttcatccg gcggattatc tccgaatgga
    13921 ggccgtcagc tttcggcagt ggatgcttgc ggatccccgg cagatggagc agaagctggt
    13981 gcggctttac ggcggcgcgg ctgaatcccc ggcgatcatt cgtctgatgg cgctggacac
    14041 accccggaag gtgcttcaaa tgggcacctg gatcatggat tatgaggcgt ggatgctgcg
    14101 ccttgcaaat gagtgtgaag ccccgtacga acgcgccata ggagctcttc gctggtcgga
    14161 tgccgagaag gtgcgcattg aaaaagaggc atccgaaagc ggcggaaatc ctctcttttc
    14221 gctctctatc ccctccatgt cggcggccta tgagcgtttt cttgcggcgg agggccagtt
    14281 ctccatggcg gctgttttga gcgcggccgt tctctatttc aatgatttcg gccgctggcc
    14341 ggtgtcgctg gatgaggttt ccatggcagc ggccggaccg cttccccgtg atcccttttc
    14401 gggtcaggat ttcttctata aaaccagccg gcttcttcct cgtgtgatgc tgagagttcc
    14461 aaaaaccatg attgatgagg gaagcattct ctaccgcatg gatatcaggg cccggcgcga
    14521 gcaggatgaa aagcagttcc agcaggccct caaaaacctt gagaaaaaat ggcgggccac
    14581 cgctgtgcgc gcggcgctgg aggttgagaa ctccccggtc ccgggaacgc cccgcaaata
    14641 ggcgcgggcc ggttcagaag ccgtttgttt ccggaaggcc tgtcagcagc accgtaaacg
    14701 actcattggt tcgaaccgtg tcgaaatccg cgccctgaag ccaggatgcg acaaacgtgg
    14761 aatcgaattt cctcaaggca ttggtcagga cctgcgccga ctgttccggc aggttttgca
    14821 gcgcatagca tgccgccaga ttgtaatagg tcacagagtt gtctggatct atttccagaa
    14881 tatcctgaaa aatttcctgc gccttgctga actctccggc gcgcatatgg gcctgtgcca
    14941 gccggttctg aatggcggtg tcgtcgcgct caagcgtggc catgcggcgc agatgcggaa
    15001 tcgccgcggc ggcgctcccg ttcagcatgt aggcgctcgc ggcaatgtcg tgggcccggg
    15061 tgttgtagtt gtcctcatcc agaatccatt ctgccatctg cgcggccggc tcataagctt
    15121 tttcagccag cagggattca gccagcagaa gccgtgtttc gtgctgtccg ggactggtgc
    15181 ggaggcattc caccaggatc gtgatggctt cggagcggtt cccttcatca agcagcaggc
    15241 gggccagccc gagcttcagg gaaacattgt ccggtgccgc cgccagttgc gcgcgcaggc
    15301 gctccgcagc aagcgcgcga tctccgccct ccgcctgctg cgccgccgca tccagaaccc
    15361 ctgcagattc ctccgccagc gcaatcagct tcgccacctg ttcttcgata tcgtccggga
    15421 gcccggcgcg ttcagtgctc tgaacgggag cgggctttgc ctgcggcatc agcactgcct
    15481 ggacacgctc ccgatgaagt gcccggcgat gacattgcgc cgcgaaaaga agaatcagat
    15541 atgccgtcag caggctgatc atcacgcccc agaatgtggc gggtgtccgg ctggtgtaac
    15601 tgcggcgcgg gcggctggca ttccggaaag atctccggtg gcggcggggc tgtatggctt
    15661 cagcctcccg ggatgccgcg tctctttttt gaggcggatc tgatgtattt ccgttttcca
    15721 ttagcgcggg gcctaccagc tgcggagctg gtcaatcacg ttttgaacgc ccgttgcgcc
    15781 ataggcgatg ccgatggcgc gggagcgcac catttcattg ggaactgaac cgcgcagggt
    15841 tactgtcgcc ccctcagcgg agacaccgaa aagatatttg ccggtcagat cgtcctcccg
    15901 caaacgattg atcacatccc gctcaagaga cgcatcccgg tccgtatagc cttcgcccat
    15961 gtcgtagacc acggtggtgc agcccgcgct gcccagcatc aggaccagcg caagagccgc
    16021 gggaatccac ttgataatat tacgcatttt ctgaactcct tccctagact ggaatgagca
    16081 taccccgcac gggcatttcg gtcaacgccc cagcctgtta ttccccgggc gatgggcgcc
    16141 ggggatagac cacgcgcatg aactggacca ggtcccccgt ctcgggccgg ccggaaacga
    16201 tgcggccggc ggcaaaagat ccgttgcgct gccccgtcat cagcagcacc gcgcatttct
    16261 gcccttcatg gaacacttcc gcttccgttc ccgcctcggg aatgatcagg cactccagaa
    16321 cggcgtgccc gttcttctgg tccacatgaa ccaccgtggc gcccggaacc cagcggatct
    16381 gcggagtgcc ggccaaaacc ggcgtcgccg ccgcgggcgg ccgcatcggc gccgttcccg
    16441 cgcatccggc gcccagaaga agggcagcgg tccagcatgt aagaagcatt ctcatgacgg
    16501 cagccattgt tccagtggac agccctcgca cagggccctt ttccggcagt gctctttgcc
    16561 cagctttacg atcaatgcgt gatattcacc atacaatgat gaatcccggg gacacgattc
    16621 cgtgaacagc gccgcgatgt cgtcgtatgc cgcgccttca tccgtccagc ggtggcgttc
    16681 cagcatgcgc cgcgtatacg cgtccacgac gaacaccggc cgcccgaatg cgtaaaggag
    16741 gatgctgtcg gccgtttccg ggccgattcc gtttgtattc agcagtatgc cgcgaagagt
    16801 gccggtttcg atctgctcca tttttttcat ggtgaagccg gatgttttcc gcacggtttc
    16861 aataaaagcc ttcaggcgtc tggccttcat gttgaaataa cccgcgggcc gtatccggcc
    16921 ggccagcgtt ttggccggca tctgtgaaag ggaccgcaca ttcagcgcgc cggacttttt
    16981 gagatttgcg atggctttct ccacgttgcg ccagttcgtg ttctgcgtga ggaccgcgcc
    17041 gaccgccacc tccagagggg tttctcccgg ccaccagtgc tgcggtcccc agcgcttgaa
    17101 aagggcgcgg tagactgcct ttgccgggag tttgctgttc ttcattgctt atatgatttt
    17161 atttgccggg ctggttctgt atacattgac ggaaacatag cgcgcggcgc gcaaaaagaa
    17221 aatcatgaat gcagaccaaa ataagcccat gaatcctgcc ggccgcagcg gcattgtcgc
    17281 cgtggtgggc cgggccaaca ccggcaaatc cacactggtg aactgcattc tggaggaaaa
    17341 gatttccatc gtcagcccgg tggcccagac cacccgcaat gtgatccggg gtgtgctgac
    17401 cgagccgcgc ggacagattg tgtttctgga tacgcccggc attcacaagg cggagtccga
    17461 gctcgggaag atcatgaacc ggtcggcgcg cgcctccacg gagggaacgg atatcatcct
    17521 gatggtattt gatgcctccc gtccgccgcg tgatgaagac gagggctggc tgcgccgagc
    17581 gctctttgaa gagattccgg tggcggctgt gctgaacaag gccgatcagc cggaagactg
    17641 ctcttcgcgc tacatggatc tctggcggga gctggagggg gaaaagcggg tgcagaagga
    17701 aatcccgtgg ctgcgaacgt ccgcgctgga gaattccggg gttcgcgaac tggtggattt
    17761 cctttttgaa cagcttcccg aggggccgca tctctttccg gatgatgtgc tgaccgattt
    17821 tccccggaag ctggccgtcg ctgacatcat cagggaaaag ctcttccacc gcctgcgcga
    17881 ggaacttccg catgcggtgg ctgtcgtggt ggatactctg gacgaggcgg ctgacggaac
    17941 ctggggcatc aagtgcacca tctatgtgaa ccggcattcg cagaaagcca ttgtcatcgg
    18001 ggaaaagggc cgcctgctgc ggtcggttcg tctggcctcg caaaaggaaa tcaggtctgt
    18061 ctatgaccat ccggtccatc tggatctgtg ggtaaaggtg gagccgaact ggtcaaagaa
    18121 tttctggctc ctcaagaagc tggggtatca gtgaagagcg gtcgaaggga attatggatg
    18181 gtgctggccc tgctggcggt cgccccggcg cttctgtatc ttccgggctt cggccacgat
    18241 tttgtctatg acgaccacgg acagcttgag gaaaacccct ttctgcagaa tccggaaaat
    18301 gtgcgcgaag tgctgatgct cgggacactg ggcgatgccc gggtgatcaa cggccgccgt
    18361 ccgttcgtgc tgctgaccta tttttcggat cgcgccgtct ggggggcgcg cgcggcggga
    18421 tatcgcctca cgaatttcgc cgcgcatgcc ggctctgtct ggctgttttt cttcctgctt
    18481 ctgcggctga gcggtcggcg ggcgttttct ttttccgcgg ccctgctgtt cagccttcat
    18541 ccggtattga tcgaggcggt gcatgtgccc gcgttccgcc cggatgtatt ctgcggactt
    18601 ttttctttcg cgtttcttct ggcggccgtc cgcacggaaa tgaaaacggc gcggcagacg
    18661 gcgctggcgg cggttctgct gtggctcgcc ctctgctcaa aggaggccgc cgcggttctt
    18721 ccggctgtac tggccggcgt ttggatatgc ctgccgcaaa cccggctgcc cgtgcgccgc
    18781 atggccctgc ttttggccgt gtcactcggg ctggtgcttc tctttatgaa actttcctcg
    18841 ggctccggcc tgcaggccgt cggcggcgcg tggaacggac gctctctcca gcccccgcat
    18901 aattttttca cctcgccgtg gctcttcctg cggatggtgg gcctgctggc tgttccgtgg
    18961 ccgctggtcg ccgaccgcgt ggtcctgccg gtaacttcca cggcagatct ccggttcgcc
    19021 gcgggcctgc tcgccgtggc ggtctgcctt tgcgcagcga ttttcttctt gcggcggaag
    19081 ccctggatca gcctcagcct tggctggatg ctggccgcct atcttccggt ctccagcctc
    19141 gttccgctgt ttaatccgct ggcggagcgc tatctctact tcatggccgc cggattctgc
    19201 attcttcctg cgcacctgct cacaagtgat ggagggtcgc gctccgccgc gaccgtttca
    19261 atgggcgtca tcgcgagcaa agcgctgctt ggatttatat gtgtgcttta tctgctcatc
    19321 ggatggatcc gcatgccggc atggaaaaac gacgttgcgc tgtggacgcg cacgctggcc
    19381 gatgagccga acagcatgcg cgcgcgcgtc tggctggcgc tcgacctgaa ggagcagggc
    19441 cggcgcgaag aggcgctgac gctgctggat gaagccatcc gcatcaatcc gcaggatgtt
    19501 tccggattca tcaatcgcgg catcctgtgg ggcgaaatgg gctactatga ccgcgcggag
    19561 gccgatctgg ccgaggcggt ccggcggcgt ccggaaagtt cttccgctca ctggaatctg
    19621 gccgtcatgc tgcagcacaa aggcgacatc gggggcatgc tccgtgaact ggaaaaaacc
    19681 cttgagctga atccggagca cgagaaggcg ctgcaggtca aaaaatccct tgaatcagcc
    19741 gtgccggaaa atcgctgaaa atcatcaaaa aacgccaaaa aataccgaaa aacggtcaaa
    19801 aaacggttct tcgtcaatta tcgtggaaag agatcgagct ttccgcagtg aaaaagga
//
LOCUS       DAOQWR010000034        19565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3151_length_19565_cov_4.898770, whole genome shotgun sequence.
ACCESSION   DAOQWR010000034 DAOQWR010000000
VERSION     DAOQWR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19565
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_19565_cov_4.898770"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKK36_04325"
     CDS             <1..738
                     /locus_tag="PKK36_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR93820.1"
                     /translation="QAVLLVGGTGTRIQSVHSDRPKGLVPVAGKPFLQWQVEWLTSQG
                     ICDIHLAAGHLGEQLAKWGMEYSAANPGICITATVEPMRLGTGGGLKFIEDEIRSDPF
                     LVLNGDSLAPNLKFQSLEVFHKKVPTIGSFSQKTSNHWKFSIAVTKIEESGRYGTVEF
                     NDDGLVTAFREKAQREAGWINAGVYMIDRGVLALMEPGRNISLETDLFPQLCKEARLA
                     AFPCPAPLLDMGTPDGLHAMECYLGRA"
     gene            814..2007
                     /locus_tag="PKK36_04330"
     CDS             814..2007
                     /locus_tag="PKK36_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR93821.1"
                     /translation="MDPINETFRSRRYDLAVDTITEGDINELIEWLKLYPRLTMADAT
                     RKFEEDWSKWLGVKYSVFCNSGSSANLLMYAALDSSGKSGNKKVIVPSVGWVTTVAPA
                     IQFGWEPAMCESDAQTFGLDIEHLKTLLESGKVDNVILVHVLGTPCRMDEILTLQKKY
                     GFKLMEDCCAAHGALSQGRKVGSMGDMSSFSFYYGHHMSTIEGGMVCTNDEELYHHLL
                     MLRSHGWCKDLPKAEYDRRMQQYGIDPFHSPFTFVVPGFNVRPMDLTARIGMMQLPRL
                     DNISSIRMKNHKLYQKLLDGQVEFARCLEGDAISSISFGAVAQSPEQRKNIVSALVEN
                     NIDTRIFSAGNLGRHPFWFERYGVFSAPMADRIHSCGFFLPNNQTLDEKNIRFICDVV
                     CRALK"
     gene            2011..3468
                     /locus_tag="PKK36_04335"
     CDS             2011..3468
                     /locus_tag="PKK36_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR93822.1"
                     /translation="MTSLNLAVIFPNNNKRALGILAQEVAAVTPPVQAALIAAHARIH
                     GFSAALLDAAQANMLPNEAVKWVEHKNPQCVLICTDFINSGDVTKMAAASETAKAIKE
                     ILPQIPVVMEGVVPTAYPERVLREEGADLVIRGEPFKPIIELLTLLKAGRRDLTSIQG
                     LCSASSMGKGECSLAAPIMDMTHFPEAAHDLIPPSLYRAHHWHCFDCLDRRTPYAAVF
                     TTVGCPYNCSFCSVGVVTDGPCFRIRPLEQVMNEIDFLVKQHGVRNLRILDNVFTARA
                     DYVERFCDEIIRRDYDLNMWAYARIESIRSPELLSKMKKAGVRWLAYGIEAANERVKK
                     AISKTTSDSATDRVIEWTKCAGIWIVGNIIFGLPEDDLQSMQESYELAVKYQFEWANF
                     YCAVAYPGTPLHRELVSRGVELPRDWAAYGQYNPNFRPLSTQYVSSEEIVKFRDDAFV
                     KYYTNPDYIRMIAGTFGQPAVEFIKKILACGNLRK"
     gene            3480..4316
                     /locus_tag="PKK36_04340"
     CDS             3480..4316
                     /locus_tag="PKK36_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNR93823.1"
                     /translation="MNKSKSVMCGKLTVKARWLWEQTIRLHGRAPETRLASSLSLVEI
                     LTALYYNGYVKSKPAELNWEGRDRVIISKGHGAITLYAILADLGYYPMNELERVGQQG
                     SFLGGIPDPIIPGFETVNGSLGHGLGVGCGMACGLRQKDNTSTSVVVIVGDGELHEGA
                     NWEAAMFAGHHRLNNVVCIVDFNKKAMLDNTDKIIGLEPLDAKFSAFGWDTFRVDGHN
                     LAALTDCFERVFKYPRKGPVAIIADTIKGRGVPVMESDPLCHVRTLSKKEIAELLGET
                     RD"
     gene            4309..5259
                     /locus_tag="PKK36_04345"
     CDS             4309..5259
                     /locus_tag="PKK36_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNR93824.1"
                     /translation="MTDRPAKRMRDMFLQGVLEEMNAATDIFFVTADFGSPVLDNIRE
                     QHPDRFLNVGIAEQNLINISTGLALEGFRVFAYAIAPFITMRCLEQTRTNLCVLASVK
                     PLSVCLVGVGAGMSYDVSGPTHQALEDISIMRSLPNMAVFSPADAECSHDLAKHVSRR
                     KGASYVRLDGKALPPIYQANERLDWNKGFNVLRKGTSASLVSTGYMTHKAMETADILT
                     KKNKRISVVDVYSLNHLNAVAFADCIKDAGPVITLEEGFTGRGGLDCLIMNAIKGRTI
                     TALGLLPEYRFDIGNRDDLLKLHGLDANTIAEKVLQTAVH"
     gene            5271..6203
                     /locus_tag="PKK36_04350"
     CDS             5271..6203
                     /locus_tag="PKK36_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR93825.1"
                     /translation="MLISVVLSFRNEADNLPELIRRLEEVFAPLEYDYELIFVNDAST
                     DGSLDLLLGARAKNPRIKILNTSRRFGVTPCVLAGFDKAAGDAIIYMDTDLQDPPELI
                     PQLIKKWEAGADIVHTTRTIRKGENAFKMWLTRQAYKLINGFSDITILQNTGDFKLIS
                     RRALNEILKLDEFDPFMRGLVAWVGFNQEQVFYERDKRYAGVTKYSLLRSLNPYKEFI
                     RGITLFSTTPLYFALLLGMIISFLSFLIFAGLVLSPLLAGDMRWTWDHLHLATLLLLG
                     GIILFTVGILGIYVGRIHRESVNRPRYLIKDWIA"
     gene            complement(6207..7721)
                     /locus_tag="PKK36_04355"
     CDS             complement(6207..7721)
                     /locus_tag="PKK36_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93826.1"
                     /translation="MGGKAFREVWNGWCDLSLGEKFICLAVLLPLIPLVGIVISRMGA
                     DYDIGAPAYQHMAMIDQILQGKVLYAPLSSEYSCVTYNPLYWYISAAFCKVFGMSFLW
                     PRLVSLMFSAGCAGIMFMWTWRLTAKDLVMSMAAPVMVLSTAFLIQFWVMEINVNALH
                     VFLVLLGFYFLIEPDMRKTVIAAIALTASFFTKQTALAYIVAAAVMLFFRSRRHLLLY
                     WVTCVIFLAAGCWYLEVFEGDFYRQTIAANQGPPWMLNRLFSEVFQQVFFGMVGCVFM
                     FSILRVFVEGSVEDVPFFARIWRSEYLLAAAGIAVEIISHPKYGSGPVHGLVAICGIT
                     LCGLSGASLIGKMAGCKKIAHATAAVQVAALIIISIPSIPSQYYDRMDEDKMIRISGI
                     FRSGRTCYFGIPYIQRKYGQASLGIPDDEPTKWVNGRLTYQFIPPKLSEPFAKQEFDY
                     VIIGSYFDPNHPVAKVIMENYKKAVAEFPAHPRFPNSTDLRHRNVVLAANRLQK"
     gene            complement(7747..8769)
                     /locus_tag="PKK36_04360"
     CDS             complement(7747..8769)
                     /locus_tag="PKK36_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93827.1"
                     /translation="MNASSSVRLSVVIPTMGRPILIKTLESLIATRDFEHIEVVVAGK
                     ISDSHVLERLQLMQKEHPQLVHLPVSFPVGDSSQKKNEGFRRSRADIVAFLDDDVYVC
                     EDWPELILKPFEDAGVGAASGPSLVPDDVKLFTRLAGTALQSKAAGYVAERYLKAGEH
                     IRPISWSRIIGCNMAYRRTVLESIGLFPPEFWPGEEMIAAWKTQQAGHRIVFYDEAWV
                     YHYPRASLWRYLKQMHGYGATRIRLLRAGVEFEPTTIVPGLWVLSLVVFGIAACFSTW
                     GSTLLLLDLFAYLLAVLYITLSKYAETRRLEHLLIFFLVPLMHLSYGIAEWVEFFRPN
                     KDLSEG"
     gene            complement(8766..11192)
                     /locus_tag="PKK36_04365"
     CDS             complement(8766..11192)
                     /locus_tag="PKK36_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93828.1"
                     /translation="MKRESMKILLAAAALFLLSLLVFHGVLDPSRILLTTDDNIGALA
                     LRERALPRGFLGWWDDSALMGLPNVLFLNWTNLLLWLLPLNFFTNWIHAIDVAMASFF
                     LLLYLRRKGLNWPACTVGILTAFWLGSNFTLTYAGHIGKFGVLVFAAAFLWLAELAVQ
                     SGKMRWAVLAGGAMGMMFLEQQDVALFFAMFLFPYFLFSMIRTGGWRPAAISRTAIPM
                     LMVALLIALRPLLAGYTANVKNVASMSQEDPRQKWEFVTQWSWPPEESIDFIAPGFMG
                     WRSGEPSGPYWGRMGRSAEWAKTGQGFMNFKLENQYLGASAVALMLLALVFSFGKNSA
                     SNPHRMEVRAWGLIALLALLLSFGKYFPLYRLFYALPVVSSIRNPNKFLQIFQLAAGI
                     LAALGLQGLLTEDMQRAKARKAVAVAGLTGLLMLFFGLLTAASWSGAVGRMAEAGWAQ
                     HAAQIVSGRMKALFYGGGMLVVFAVWIWLLPSRKFRWMPWFACMLLMADVFYLSRYYI
                     QTMSRNDVAENEVVQFLKQESARTYQRAALADQSGFYNHWLTYLFPYHGINTINITQM
                     PRMPEDYKAFLNAVGSNPLRYWGLCGVGPVLVPAGIWTQMQRDPAVRDKFDIRFAYSV
                     AMDQGSMKIIPGAEATPGRHAILQSKTSLQRFALIPKWETLPDDQALMRLKSASHDAK
                     STVLVSTETPLGKPSNPDGVVNGTVTVKHYSAGRVEIQVSADEPCLLRLADKWSPEWE
                     VLLDGHPVDLLRCDYLFQGVYIPPGLHWVKMRFAPSVATLWVQLTGLAVCLAAAVTLA
                     ADRFKTGPAR"
     gene            complement(11189..13162)
                     /locus_tag="PKK36_04370"
     CDS             complement(11189..13162)
                     /locus_tag="PKK36_04370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR93829.1"
                     /translation="MVFGVTALGIAAAAAFFRLHDLGMSAFRADTILLWSLAQRHIPP
                     VQLWTQWFEVSGAAGQMPMPAWIMQLFLSLTEWPVTPFWVRFPHALLGMLAVPVAYAG
                     ACRMGGKGMGLVLAALVAVNPYHIGYSREAYFYASAFLGYFLFFWAMVALTHEIRGGH
                     GVRWKTLLLLAAALFFTAYSQITGLILCAAGALILGAVLFLNREGCDQPWRSLGCIAG
                     VYSIVLAPLVFAAWGLKPLLSQIGANREAGAQVVALSGERLLPSVGTALLRFAWGNDA
                     WAVGLLVLSLALAVAVMIRRKEKHLVWIPGLMVLQLVLFALSRKASGATYDPRYMSGI
                     MPFYLALIAYGLWNLPSLLPRKAGPFAWIACGAAVAAGVPAAYLQTRVTGKPTPYFEI
                     VRWTDSNLPAGTPVLVDRWFEPWNELKAHPSTNVQFTFTIPNEPLDNFLKGNWRKTAE
                     EFLVRNPDAAYLEIAKTYWDAPGVGPWEWPRKYFARHVTIANEAGLKLRKLGLATRSD
                     FYSSNSNRVVVEIFYNTEEDVIAKARADGRSVLALYGPGWQYTKTGDYRDWRTIPEKA
                     EMRLWNLTDGPLTASPVLRAAAMGGPVRLRAKAPALQVRDFPPNQLTEWTLEPMTLQP
                     GMTTVILTPEAQPNRGLLVEHIRASVIQSGEPE"
     gene            13334..14143
                     /locus_tag="PKK36_04375"
     CDS             13334..14143
                     /locus_tag="PKK36_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR93830.1"
                     /translation="MIAPETLEYLPTDGADYLRHTDFNRSPTHRNRLGCLVKMVGRCG
                     PAQPRILEIGCGIGNISIPLASLGGTITAIEPHEPSLQIAASRNPFPNLTFKHQSLEE
                     TELGAFDIIILTEVLEHVPDHKAMMKHLARGMRPDARLIVTLPNGRSLTERLCRPSYA
                     LKRTAAGTALVRAIKKLLGARDITTANATTPHVHFFSLFEADRLFTECGFDVLIFQRY
                     FRHWLLKETFFTKYSHSEDAARSDFVKSQHIRPEKCSLWAFLLKPSGGGPS"
     gene            14140..>14384
                     /locus_tag="PKK36_04380"
     CDS             14140..>14384
                     /locus_tag="PKK36_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR93831.1"
                     /translation="MTGVSYDDLWSGTWNDTTRYGPACRHRRRIVAKLVLRLPHQTIC
                     DLGCGDGRLLSELSRKTSAALFGADVSATAVEQAGKNV"
     assembly_gap    14385..14484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14485..14731
                     /locus_tag="PKK36_04385"
     CDS             <14485..14731
                     /locus_tag="PKK36_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93832.1"
                     /translation="KVMNEGGFDVIFYRRWGFPFHEILQFIMSRGQDGKEVQDGAYTR
                     LKIIIANILYGIFFLNVLPMGRQVFAVGRSREYSGNT"
     gene            14728..15894
                     /locus_tag="PKK36_04390"
     CDS             14728..15894
                     /locus_tag="PKK36_04390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR93833.1"
                     /translation="MKILLVSNYQPPHMGGIEFAAGALKRCWERDGHTVTWLTTDIPR
                     GGQPSTAENVRIPACNVFERLWEINSPIVSPCGYPRIRKLIREHDIVNIHSLAPGLSS
                     VCLSAAIRCRRPAVVTQHVAIIPLASKLLTAVQHHVICAAARRAVRQDIPLTFVGEAV
                     RRWFIEESGIPAEMTHMTPAGIEPDKYFLVPDSERAALMRKWNLPENQFKVLFVGRFY
                     EKKGLQLIRKVAEQCPDILFSFRGSGPDDPEAWKLPNVRILPMVTDEELREIYGCHDL
                     FIMPSIGEGWPAVIPQAMACGLGCMISEETFQGYNRDRDRFIVVPRSADAIVKQLNDA
                     KEDRIPWLKNRVGISDYAVRTWNWQQTARIYSEIFEKQVQNAGRSDGRQADLHE"
     gene            complement(15862..16794)
                     /locus_tag="PKK36_04395"
     CDS             complement(15862..16794)
                     /locus_tag="PKK36_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNR93834.1"
                     /translation="MMHEIYPLRFEPVYRDYVWGGDRIVRKYRRGLPPGIYAESWEIA
                     DRPDGMSVVANGRLKGKTLHQLTQTMGEVLLGAGRRDESFPLLVKIIDAHECLSVQVH
                     PDDEGAARFGGEAKTEMWYALEAGPGAKVYAGLKKGVSPETLRSAIENHAVADCLKTV
                     PMGKGTAVYVPGGRIHAIDAGCLLLEIQQNSNTTYRIYDWGRPRELHLEQALNVLRPN
                     DDEDPRVDAGEFQCKYFIFRTHHLNDSKSFRNDGRSFHILFAEEGPLQIRWTGGTEDI
                     EEGTTLLIPAALKEWTVVPQRNAGSLVQISLPSV"
     gene            complement(16791..18842)
                     /locus_tag="PKK36_04400"
     CDS             complement(16791..18842)
                     /locus_tag="PKK36_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNR93835.1"
                     /translation="MTKDFLLNLGQYRKLVEQLPAISYIVELGPVNRTVYISPQVESI
                     LGFTPAEWMADPHLWIRQLVDEDRARITNEVYRKNATGGSFFLVYRVRARDGRIKWFR
                     NHAAYMDDENGEPRYVYGVMLDVTNYRETEEALRQSQIGYRRIFESIQDVYFELDLDG
                     RVRAVSPSVAALTGFEPEELKNRPFQAICVQPEQVAVLLTALRQDGILEDAEVTVIHK
                     AGAIEYCSLNARLLQDEPDSPVIATLHKITERKHVEMELQHARDNLERRVQERTAELL
                     KANRQLRAEILERKEAETALQESRSRLVQSQKMDALGQLVCGISHDFNNLLTTIMGYS
                     QLAMTGLPEDGPVQQDMAQVYQECCRAARLTQKLLSIGRSRLPDIQPLDLNEIVDEMA
                     PFMQRTLGERIQLHIRKSALGESVLADSSMLEQIILNLAVNARDAMPDEGFLTVETGS
                     CPCSDTPLREAEPGGLCVFLSVKDTGCGMPDEVRTRAFEPFFTTKKQGEGSGLGLAMV
                     ADFMRQLGGAVVLDSQPGKGSEFRLYFPCSHLRAEHREVPEARPAPRGNECVLVLEDD
                     DTVRHLTSRLLASLGYQALEAETEAEAEFILDQDPAQIHLVFCDVIMPKANGPEIIAR
                     LRIKHPGLRVLFTTGFSTDMAVRAGIPSDQVKCILTKPYSRFSLAVRLREVLDRNEGE
                     L"
     gene            complement(18939..19166)
                     /locus_tag="PKK36_04405"
     CDS             complement(18939..19166)
                     /locus_tag="PKK36_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93836.1"
                     /translation="MARNYHVKGTRSYLIASVVLVVLAAWFIKDGWFPSEHWLTKYPD
                     PADNFYAFNKTTGVLMAIAAGVCALIHIKVR"
     gene            complement(19220..>19565)
                     /locus_tag="PKK36_04410"
     CDS             complement(19220..>19565)
                     /locus_tag="PKK36_04410"
                     /inference="COORDINATES: protein motif:HMM:NF020334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNR93837.1"
                     /translation="IPKVYRVWSDAPLTAEDVQKFLAGIQHDGECLTAAAVRLAPDDR
                     NCAEVTLLEGKNRQIRRMYAAAGKKVERLCRIRIGPLKLDALRSGQWRPLAQAEVAEL
                     KKAAGAERAGQA"
BASE COUNT         4617 a         5592 c         5103 g         4153 t
ORIGIN      
        1 caggcggttc tccttgtcgg cggcacgggc acgcggatac aatccgtgca ttccgaccgg
       61 cccaaggggc tggttcccgt ggcgggcaag ccgttccttc agtggcaggt cgaatggctg
      121 acatcgcagg gcatctgcga cattcacctc gcggccggac atcttggaga gcagctcgct
      181 aaatggggca tggaatacag cgcagcaaat cccggcatct gcatcacggc caccgtcgaa
      241 cccatgcgcc tgggaaccgg cggcggattg aaattcatcg aggacgaaat ccggtccgat
      301 ccatttctcg ttctaaacgg ggacagcctt gcgccgaacc tgaagttcca atcattggaa
      361 gttttccaca aaaaagttcc aaccattgga agtttttcgc agaaaacttc caatcattgg
      421 aagttttcga ttgcggtgac aaagattgaa gaatccggcc gctacgggac cgtggagttc
      481 aatgacgatg gactcgttac agccttccgc gaaaaagctc agcgcgaagc cggctggatc
      541 aatgccggag tctacatgat tgaccgcggc gtactggctc tcatggaacc gggcagaaac
      601 atatcccttg aaacggacct tttcccgcaa ctctgcaaag aagcccgtct cgcggccttc
      661 ccctgccccg cccccctgct ggacatgggc acgccggacg gactgcacgc catggaatgt
      721 tatctggggc gggcataatt gaattgcaga aatggcttat aacgggtaca ttgcggtgtt
      781 ttgaactggg aaaaatgact gcgggagaat gcattggatc caatcaacga gacatttcgg
      841 agccggcgct atgatctggc cgtggacacc atcaccgaag gtgacatcaa cgaactgatc
      901 gagtggctga agctgtatcc gcgccttaca atggccgatg caacccggaa gtttgaggaa
      961 gactggtcaa aatggctggg agtcaaatat tccgtgttct gcaattcggg ttcctccgcg
     1021 aatttgctga tgtatgcagc gcttgattcc agcgggaaat caggaaataa gaaagtgatc
     1081 gtcccctcgg tcggctgggt caccaccgtt gcccctgcaa tccaattcgg ctgggagccc
     1141 gccatgtgcg aatcggatgc acagactttt ggcctggata tcgagcacct gaagacgctc
     1201 ttggaatccg ggaaggtgga caatgtcatt ctggtccatg tgctgggaac gccctgccgg
     1261 atggatgaaa ttctgacact tcagaagaaa tacggtttta agctgatgga agactgctgt
     1321 gcggcccacg gtgcgctttc acaggggcgc aaagtcggct ccatgggcga tatgtcatcc
     1381 ttttcatttt actacggcca tcacatgtcc acaatcgaag gcggcatggt ttgcacaaac
     1441 gatgaggagt tgtatcacca tttgctgatg cttcgctcgc acggctggtg caaagacctg
     1501 cccaaggcgg aatatgaccg gcgaatgcag cagtacggaa ttgatccctt ccattcacca
     1561 ttcacttttg tggtgccggg cttcaatgtc cgccccatgg acctgactgc acggatcgga
     1621 atgatgcagc ttccgcgact ggacaacatc tcgtccatcc gcatgaaaaa ccacaaactc
     1681 tatcagaaac tgctcgatgg ccaggttgaa tttgcccgct gtctcgaggg cgacgccatc
     1741 agcagcattt ccttcggtgc cgtggcccag tcgccggaac agcgcaaaaa tattgtcagc
     1801 gccctggtcg aaaacaacat tgatacaaga atcttctcgg ctggcaacct cgggcggcat
     1861 cctttctggt ttgagcgcta cggcgtcttt tccgccccca tggccgaccg gattcacagt
     1921 tgcgggttct ttcttccaaa caaccagacg ctggatgaga aaaatatccg ttttatttgc
     1981 gatgtcgttt gccgcgcatt gaagtagaga atgacctcgc tcaacctagc cgtcatattc
     2041 ccgaacaaca acaagcgggc tctcggcatt ctggcgcaag aggttgcggc tgtcacacca
     2101 ccggttcagg ccgccctcat agcggcccat gcccgaatac acggattttc agctgccctg
     2161 ctggatgcag cacaagcgaa catgcttcca aatgaagcgg tcaaatgggt ggaacacaag
     2221 aatccacagt gcgtgctgat ttgcaccgac ttcatcaatt ccggggacgt cacaaagatg
     2281 gccgccgcat cggaaacagc caaggccatt aaggagattt tgccgcagat tccagtcgtg
     2341 atggaaggtg ttgttcccac ggcatatccc gaacgggttc ttcgcgaaga aggcgccgat
     2401 ctggttatcc gcggggagcc ctttaagccc ataattgaac tactgacact cctgaaggcc
     2461 ggccgccggg acctgacttc gattcaaggc ctctgctcgg catccagcat gggaaaagga
     2521 gaatgcagcc ttgcggcacc aatcatggat atgacccact tcccggaggc cgcgcatgat
     2581 cttattcctc cttcgcttta ccgcgcccat cactggcatt gtttcgactg cctcgaccgg
     2641 cgcacgccgt atgccgcggt gttcacaaca gttggctgtc catacaactg ttctttctgc
     2701 agtgttgggg ttgtaaccga cgggccctgc ttccgcattc gtccgctgga acaggtgatg
     2761 aacgaaattg attttctggt gaaacaacac ggtgtacgga acctccgtat tctggacaat
     2821 gtctttaccg cacgggcgga ctacgtggag cgcttctgcg acgaaatcat ccgtcgcgac
     2881 tacgatctga acatgtgggc ctatgcacgc attgagtcca ttcgcagccc cgaactgctt
     2941 tccaaaatga agaaggccgg ggtgcgctgg ctcgcctatg gaattgaggc ggccaatgag
     3001 cgcgtgaaaa aggccatctc caaaaccacc agtgattcag cgactgaccg cgtcatcgaa
     3061 tggaccaagt gcgccggaat atggattgta ggcaatatta ttttcgggct gccagaggac
     3121 gacctgcaga gcatgcagga atcttacgaa ctcgctgtga aatatcagtt tgagtgggcc
     3181 aatttctatt gtgctgtggc ttatcccggg actccgcttc accgcgagct ggtttcccga
     3241 ggcgttgaac tcccgcgcga ctgggccgct tacgggcagt acaatccgaa tttcaggccc
     3301 ctttcaaccc agtatgtctc atcggaagag attgttaaat tccgcgatga cgcctttgtg
     3361 aaatactaca caaatccaga ttatattcga atgatcgcgg gaacatttgg gcaaccggcg
     3421 gtcgagttca tcaagaaaat ccttgcctgc ggaaatctgc gaaagtagct taaaccgcga
     3481 tgaacaagtc caagagcgtc atgtgcggaa aactgaccgt aaaagcccgc tggctttggg
     3541 agcaaaccat ccggcttcac ggacgggccc cagaaacacg cctggcctcc tcactttcac
     3601 tcgtcgaaat tctgacggcg ctttactaca acggatatgt taaatccaag cccgccgaac
     3661 tcaactggga gggacgagac cgggtgatta tcagcaaagg acacggcgcc atcacacttt
     3721 acgccattct cgcagatctg ggctattacc ccatgaacga gctggagcgg gtcggccaac
     3781 agggatcatt tctgggcgga attcccgatc ccatcattcc gggttttgaa accgtaaatg
     3841 gttctctggg ccacgggttg ggcgtgggct gcggcatggc ctgcggactg cggcagaagg
     3901 acaacacctc tacgagcgtg gtagtgatcg tcggagacgg agaacttcat gaaggagcca
     3961 actgggaagc ggccatgttt gcgggccatc accggctcaa caatgtggtc tgcattgtgg
     4021 atttcaacaa gaaggccatg ctcgataata cggacaagat cataggactg gaaccgctgg
     4081 atgccaaatt ttccgccttt ggctgggaca cattccgtgt ggacggacat aatctcgcgg
     4141 ccctgacaga ctgcttcgag cgggtattca agtatccgcg caagggtccc gtggcaatca
     4201 ttgccgacac gatcaagggg cgtggtgtac cggtcatgga gagcgaccct ctctgccatg
     4261 tgcgaacact ttctaaaaag gaaatcgccg aactgctggg agaaacccgt gactgatcgt
     4321 cccgccaagc gaatgcgcga tatgttcctg cagggcgtgc tggaggaaat gaatgcggca
     4381 acggacatat ttttcgtcac ggcggatttc ggatcgcctg tactggacaa tatccgggaa
     4441 cagcatcctg accgattcct caacgtgggc attgccgaac agaatctgat caatatttcc
     4501 acgggtcttg cgctggaagg atttcgtgta tttgcctatg ccatagcacc gttcatcacc
     4561 atgcgatgtc ttgaacaaac tcggaccaat ttgtgcgtgc tggcttcggt taagcccctc
     4621 tccgtctgcc tggtcggggt cggagcaggc atgagttatg atgtttctgg ccccactcat
     4681 caggcgcttg aggatatctc catcatgcgg tcgctgccta acatggctgt tttttcaccg
     4741 gcagatgcgg aatgctccca tgatctggca aagcatgttt cccggcgaaa gggtgcctct
     4801 tatgtgcgac ttgacgggaa agcgctaccc ccaatctatc aggcgaatga acgccttgat
     4861 tggaacaaag gttttaacgt tctgagaaaa ggaacctcag catcactggt ttccaccggg
     4921 tatatgacgc acaaagcaat ggagactgcg gatatcctca ccaaaaagaa taagcggatt
     4981 tcagtcgtag atgtttattc cctgaatcat cttaatgccg tggcatttgc cgactgcatc
     5041 aaagatgcgg gcccagtcat cactcttgag gagggcttta cggggcgcgg cgggctcgac
     5101 tgcctgatta tgaatgccat aaaagggcgg acgattacag cactgggact cctgcccgaa
     5161 tatcgttttg acattgggaa tcgggatgat cttctgaagt tacacggtct cgacgccaac
     5221 accattgcgg aaaaggttct tcagactgca gttcattaaa ggggaacatc atgcttatat
     5281 ccgttgtgct ttcatttcgt aacgaggcag acaatttgcc ggaactgatt cgccgactgg
     5341 aagaagtttt tgccccgttg gaatacgatt acgaactgat ttttgtcaac gatgcctcaa
     5401 ctgacggctc tcttgatctt ctccttggtg cgcgcgcaaa gaacccccgc atcaagatcc
     5461 tcaacacttc acgccgattc ggggtcacgc catgtgttct ggccggtttc gacaaggctg
     5521 caggcgatgc catcatctac atggatactg atttgcagga tcctccggaa ctgattccac
     5581 aattgattaa gaaatgggaa gccggcgcgg atattgttca tacaacgcgc acaattcgaa
     5641 agggagagaa cgcattcaaa atgtggctga cccgtcaggc ctataagctc attaatggat
     5701 tttccgatat cacaattctg cagaacaccg gagattttaa gctcatctca cggcgggccc
     5761 tgaatgaaat actgaagctg gacgaatttg acccctttat gcgcgggctg gtggcgtggg
     5821 tcgggtttaa tcaggaacag gtcttttacg agcgtgataa acgttatgcc ggcgtgacca
     5881 aatattccct cttgagaagc ctgaatccct acaaggagtt cattcggggc attacgctgt
     5941 tttccaccac ccccctctat ttcgcccttc tgctggggat gattatatcc tttctttcgt
     6001 tccttatatt cgccggcctg gttctgtcac ccttgctggc cggagatatg aggtggacat
     6061 gggaccatct tcatctggct actctgcttc tcctgggggg gattattctt ttcacggtgg
     6121 gtattctggg catctatgtt ggccggatcc atcgcgaatc ggtcaatcgc ccccgctatc
     6181 tgatcaaaga ctggattgcc tgaatattat ttctgcaacc gattggcggc aagaaccaca
     6241 ttgcggtgac gcaagtccgt gctgttgggg aatcgcggat gtgcgggaaa ctccgcaacg
     6301 gctttcttgt agttctccat aatcactttt gcaaccggat ggttgggatc aaaatatgac
     6361 ccaatgatga cgtaatcgaa ttcctgtttc gcgaacggct cagaaagttt gggcggaatg
     6421 aactgatagg tcagtctgcc gttcacccac ttggtcggtt catcgtccgg tatccccaga
     6481 gacgcctgtc catactttct ctgaatgtaa ggaataccga aatagcatgt gcgcccagat
     6541 ctgaaaatac cgctgatccg gatcatctta tcctcatcca ttcggtcgta atactgactg
     6601 gggatgctgg gtatcgatat aattatcaga gcagcaacct gaacagcagc tgttgcatga
     6661 gcaattttct tacatccggc cattttccca atcagggagg cccccgacaa accgcacaaa
     6721 gtgatcccgc aaatcgccac cagtccatgc accggaccac tcccatactt gggatgagat
     6781 ataatttcaa cagcgattcc cgcagccgcc agcagatatt cgcttctcca tatccgcgca
     6841 aagaacggga catcctcaac agaaccttcg acaaatacgc gtaatataga aaacataaac
     6901 acgcatccga ccattccgaa aaatacctgc tgaaatactt cagagaacag acgattgagc
     6961 atccacggcg gcccctgatt ggcggctata gtctgacgat agaaatctcc ttcgaagact
     7021 tccagatacc agcatcctgc agcgaggaat attacgcagg tgacccaata aagcagcaaa
     7081 tgccggcggc tccggaagaa aagcatcaca gcggccgcca ctatatatgc caacgcagtc
     7141 tgctttgtga aaaaggaagc cgtcagcgca atcgccgcaa ttacggtttt ccgcatatct
     7201 ggttcaatca gaaaatagaa gcccagcagc acaagaaaca catgcagagc attcacattg
     7261 atttccataa cccaaaactg aatgagaaat gcagtgctga gcaccatcac aggcgcagcc
     7321 atgctcataa ccagatcctt tgcagtcaaa cgccatgtcc acatgaacat aatgccggca
     7381 catccggcag agaacatgag gctgaccagt cggggccaga gaaaactcat tccaaacacc
     7441 ttgcaaaatg ccgcgcttat gtaccagtaa agagggttat acgtcacaca actgtattca
     7501 gaagacagag gcgcatacag caccttgccc tgcagaatct gatcaatcat ggccatatgc
     7561 tgatatgcag gagcgccaat atcataatcc gcacccattc ggctgataac gatgccgaca
     7621 agcgggatta aaggcagaag aacagccaaa cagataaact tctctcccag cgacaaatcg
     7681 caccagccat tccacacttc acgaaatgcc tttcctccca taatatgcag tctcccttac
     7741 tctgactcag ccctcgctca aatccttgtt tgggcgaaag aactccaccc attcagcaat
     7801 gccataactc aaatgcatga gcggaacgag aaagaaaatg agcagatgct caagccggcg
     7861 ggtctcggca tatttgctga gcgtgatata aagcacagcc agcagatagg cgaacagatc
     7921 cagcaggagc agcgtgctgc cccatgtcga gaagcatgcc gcaatcccaa acacaaccag
     7981 agaaagcacc catagacccg gaacaatggt ggtgggctca aattcgacac cggcccggag
     8041 cagacggatg cgggtggcgc cataaccgtg catttgtttc aaataacgcc acagtgacgc
     8101 gcggggataa tgatagaccc atgcttcatc ataaaacacg attctatggc cggcctgctg
     8161 ggttttccac gcggcaatca tttcctcccc gggccagaat tccgggggaa acaacccgat
     8221 ggactccaga actgttcggc ggtacgccat gttgcagccg atgattcggc tccaggatat
     8281 gggccggata tgctccccgg ccttgagata ccgctcggcc acataaccag ccgccttgga
     8341 ctgcagcgcg gtcccggcca gccgcgtgaa tagtttcaca tcatccggga cgaggctggg
     8401 cccgcttgcc gcgcccacgc ctgcatcttc aaaaggcttg aggatcagtt caggccagtc
     8461 ctcgcacaca taaacgtcat cgtccagaaa agcgacaatg tcggcccggg agcggcggaa
     8521 gccctcgttc ttcttctggc tggaatcgcc gacaggaaag gataccggca gatgcaccag
     8581 ctgcgggtgc tccttctgca tgagctgaag cctctcaagc acatgggaat cagatatttt
     8641 acccgcaacg accacttcga tgtgttcgaa atcccgggtg gcaatcagtg attccagtgt
     8701 tttgatcaga atcgggcgtc ccatggtcgg aatgacaacg gacaaccgga ctgacgaaga
     8761 agcattcatc gagcgggccc cgtcttgaaa cggtccgccg ctaaagtgac ggctgcggcc
     8821 agacagacgg ccaggcccgt cagctgcacc cacagggttg cgacagacgg cgcaaagcgc
     8881 atcttgaccc aatgaagccc cggaggaata tagaccccct gaaatagata gtcacaacgg
     8941 agcaggtcaa cgggatgacc atccagcaaa acctcccact ctggcgacca tttgtcggcc
     9001 aggcgcagaa gacatggttc atcggcggac acttgtattt ccacacggcc ggcactgtaa
     9061 tgcttcactg tcactgtgcc attaaccacc ccatcaggat tcgagggttt tcccagcggg
     9121 gtttcggttg aaaccagtac agtggatttc gcatcatggg atgcgctctt caaacgcatc
     9181 agcgcctgat catcaggcag ggtttcccat ttcgggatca gcgcgaagcg ctgcagggaa
     9241 gttttcgact gcagtatggc atggcgaccg ggggtggctt ctgcaccggg gatgattttc
     9301 atgcttcctt gatccattgc aacgctgtat gcgaagcgga tatcgaattt atccctcacg
     9361 gcgggatcgc gctgcatctg cgtccagatg ccggcgggca cgagcaccgg ccccaccccg
     9421 cacagccccc agtatcggag aggatttgaa ccaaccgcat tcagaaaggc tttgtaatcc
     9481 tccggcatgc gcggcatctg cgtgatattg atcgtgttta tgccatgata agggaaaaga
     9541 taggtcagcc agtgattata gaacccggac tggtcggcca gtgctgccct ctggtatgtc
     9601 cgcgccgatt cctgcttcag aaactgaaca acctcgtttt cggccacgtc attccggctc
     9661 attgtctgga tgtaatatcg tgaaagataa aaaacgtctg ccatcagcag catgcaggcg
     9721 aaccagggca tccagcggaa cttccgggag ggaagcagcc atatccagac agcaaagaca
     9781 acaagcatgc ccccgccgta gaaaagggcc ttcatgcggc cgctgactat ctgtgcggcg
     9841 tgctgggccc agccggcttc ggccatgcgg ccaacggccc cgctccatga agccgccgta
     9901 agcagaccga aaaacagcat cagcaggccg gtcagaccgg caacggcaac ggctttgcgc
     9961 gcttttgctc tttgcatatc ttccgtgaga agcccctgca ggcccaatgc ggccagaatg
    10021 cccgccgcaa gctgaaatat ctggaggaat ttgttcggat ttcgaataga ggacacaacc
    10081 ggcagcgcat aaaacagccg ataaagagga aaatattttc caaaggacag cagcagcgcc
    10141 agcagcgcaa tgaggcccca tgcccggacc tccatgcgat gtggattcga tgcagagttc
    10201 ttgccaaagg aaaacaccag cgccaagagc atcagcgcca cagcggaggc tcccaaatac
    10261 tggttctcaa gcttgaagtt catgaacccc tgccccgttt tcgcccactc ggcagaacgg
    10321 cccatccttc cccagtaagg acctgagggt tctccgctac gccagcccat gaagcccggc
    10381 gcaatgaaat caatggattc ctccggaggc cagctccact gggtcacgaa ctcccatttc
    10441 tggcgaggat cttcctgcga catgctggcc acgtttttca cattggcggt gtatcctgca
    10501 agaagggggc gcaaagcaat cagcagtgcc accatcagca tgggaatggc tgtgcgggag
    10561 atagccgccg gccgccagcc gccggtgcga atcatgctga acagaaaata tgggaacaaa
    10621 aacatggcaa aaaacagagc cacgtcctgc tgttcgagga acatcatgcc catggccccg
    10681 cccgccagca cagcccagcg cattttcccc gactgaaccg ccagttcagc cagccagaga
    10741 aaggcggcgg cgaagacaag cacgccgaat ttgccgatat gcccggcata ggtcagcgta
    10801 aagttgcttc ccagccagaa ggccgtcaga atgccgaccg tgcatgcggg ccagttcaac
    10861 cccttgcgcc gaagatagag caacaggaaa aaagatgcca ttgcaacatc aatggcatgt
    10921 atccagttcg taaaaaagtt cagagggagc agccagagaa gcaggttggt ccagttcagg
    10981 aagagcacgt ttggaaggcc catgagcgct gaatcatccc accagcccag gaagccccgg
    11041 ggaagagctc tttcacgcag ggccagagcc ccgatattgt catccgtggt cagcagtatg
    11101 cgtgaagggt caagaactcc atggaaaacc agaagcgaca gcagaaaaag ggccgcggcc
    11161 gcaagaagaa ttttcattga ttcgcgtttc attcaggctc tcctgattga atcaccgacg
    11221 cgcggatatg ctcaacaagc aggccccggt tgggctgtgc ttcgggggtc agaatcactg
    11281 ttgtcattcc cggctgaagc gtcattggct cgagcgtcca ttccgtcagc tgattcggag
    11341 ggaagtcgcg cacttgaagt gccggagctt ttgcgcgcaa acggacgggc ccgcccattg
    11401 ccgccgcccg gagaaccgga cttgctgtca acggtccatc tgtcagattc cacaggcgca
    11461 tctctgcttt ttcgggaata gtgcgccagt cgcgatagtc tccggtcttg gtgtactgcc
    11521 agccggggcc atacaaagcc aagacagaac ggccatcagc tcttgccttt gcgatcacat
    11581 cctcttcggt attgtagaag atttccacca ccacgcgatt tgagttggat gaataaaaat
    11641 cgctgcgcgt ggccagcccc agcttgcgca atttcaatcc cgcttcattt gcaatggtaa
    11701 cgtgccgcgc aaaatacttg cgcggccatt cccacgggcc cactccggga gcatcccagt
    11761 aggtttttgc aatttcaaga taagccgcgt ccggatttct cacaagaaac tcctcggccg
    11821 tctttctcca gttgcctttc agaaaattgt ccagcggctc gttcgggatt gtgaacgtga
    11881 actggacatt tgtggaggga tgggctttca gctcgttcca gggttcaaac cagcggtcca
    11941 ccagcaccgg cgtgccggcg ggaagattgg agtcggtcca tctaacaatc tcgaaatagg
    12001 gcgtcggctt tccagtgacc cgcgtctgaa gataagcggc gggaactccg gccgcgacgg
    12061 cagctccaca cgcaatccaa gcgaacgggc cggcttttcg aggcagaagc gagggaagat
    12121 tccacagccc atacgcaatc agcgccaggt aaaacggcat gattccggac atgtatctcg
    12181 gatcatatgt ggccccggat gccttgcggg aaagcgcaaa cagcaccagc tgcaggacca
    12241 tcaggccggg aatccaaaca agatgtttct ctttccgccg gatcatcacg gcgacggcca
    12301 gagccagcga cagcaccagc aagcccaccg cccaggcatc gttgccccaa gcgaagcgca
    12361 gcagggccgt cccgacactg ggaagcaggc gttctccact cagagccaca acctgggccc
    12421 cagcctcccg gttggcgcca atttgcgaaa gcagcggctt caaaccccat gccgcaaaaa
    12481 caagcggagc aagcacaatt gaataaactc ccgcaataca gccgagactt ctccacggct
    12541 ggtcgcaacc ttcgcgattc agaaacagga ccgcacccag aatcaaggct cccgcagcgc
    12601 aaagtatcag acccgtgatc tggctgtaag ccgtgaaaaa cagagcggcg gcaagcagca
    12661 aaagagtctt ccatcgaacg ccgtggccgc ctctgatttc atgagtcaaa gccaccattg
    12721 cccagaaaaa aaggaagtaa cccagaaagg cggatgcgta gaaataagct tctcttgaat
    12781 aaccaatatg ataagggttc accgcgacca gcgccgccaa aacaagtccc atgcccttac
    12841 cgcccatgcg acaggcacca gcgtatgcga caggcacggc cagcattccg agcaaggcgt
    12901 gagggaatcg cacccagaaa ggcgtaacag gccattctgt caggcttaga aacagctgca
    12961 taatccatgc aggcatgggc atctggccag ccgcacccga cacttcaaac cattgcgtcc
    13021 acagctgcac tggcgggata tgccgctggg ccaggctcca cagcagaata gtatcagccc
    13081 tgaacgcaga catgcccaga tcatggagac ggaagaatgc cgccgcggcc gcgatcccca
    13141 aagccgttac gccaaacacc atatttcgcc ggtcagccgt cataatcaac cattagagtc
    13201 agttcggtgc atatcctgaa tgcagagcag gcgacgggca agtctaaact cagggcctcg
    13261 cccgccagaa tgcgcattga cgcgttcggg ccctgtgcgt atcgtctccg atgaatattc
    13321 agctacggaa aacatgattg cgccggaaac acttgaatac ctgccgacag atggggcgga
    13381 ttatctgcgc cataccgact tcaaccgcag tcccacccat cggaaccggc tgggctgcct
    13441 cgtgaagatg gtcggccggt gcggcccggc tcagccccgg atactggaaa tcggttgtgg
    13501 aatcgggaac atctcgattc ccctagccag tcttggcggc accatcaccg cgattgagcc
    13561 gcatgagcca tcgctgcaaa ttgccgcatc gcgcaatcca ttccccaatc tcacctttaa
    13621 gcatcagtct ctggaagaaa cggaattggg ggcttttgac atcatcatcc ttactgaagt
    13681 tctggaacat gtgcctgatc acaaagccat gatgaaacac ctggcgcgcg gcatgcggcc
    13741 ggacgcgagg ctgattgtca ccctgcccaa cgggaggtcc ctgaccgaac gtctctgccg
    13801 ccccagttac gccctgaagc gcacagcggc aggcactgcg ctggttcgcg caatcaaaaa
    13861 gctgctcggc gccagagaca tcaccaccgc caatgccacg actccgcacg ttcatttctt
    13921 cagcctgttt gaagccgacc gtcttttcac cgaatgcgga tttgacgtgc tgatcttcca
    13981 gcgctatttc cgtcactggc ttctgaaaga aaccttcttc accaaataca gtcattcaga
    14041 agatgcggca cgttctgatt ttgtaaagtc acagcatatt cggcccgaga aatgctctct
    14101 ttgggccttt cttctcaagc cttccggagg gggaccgtca tgaccggcgt cagctatgat
    14161 gatctgtgga gcggcacatg gaatgacaca acccgctatg gcccggcctg ccggcaccgc
    14221 aggcgcattg tcgcgaaact cgtcctccgc cttccccatc aaaccatctg cgatctgggg
    14281 tgcggcgacg gccgcctgct gtctgagctc tcccgaaaaa catccgccgc tctttttggc
    14341 gccgatgttt ccgcaaccgc cgtagagcag gccgggaaaa acgtnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnaaaagt gatgaatgag ggcggttttg atgtgatttt
    14521 ctatcgtcgc tggggattcc ctttccatga aatcctgcag ttcataatgt cacgcgggca
    14581 agatggaaaa gaagtgcagg atggcgctta cacccgcttg aaaataatta tcgcgaacat
    14641 cctctacggc atattttttc tcaatgtgct gcccatgggg agacaggttt tcgccgtggg
    14701 ccggagccgg gaatattcgg gaaatacatg aagattctgc tggtctcaaa ttatcagccg
    14761 ccccacatgg gcgggataga gtttgccgct ggtgcgctga aacgctgctg ggagcgggac
    14821 gggcacactg taacatggct gaccaccgat atcccccggg gtggacagcc ttccactgct
    14881 gagaacgtaa ggattcccgc atgcaacgtc tttgaacggc tttgggaaat caattcgccg
    14941 atagtaagcc cctgcggcta tccccggatt cggaaactca ttcgagaaca cgacatagtc
    15001 aacatccatt cgctggcgcc ggggctttcg tcggtttgtc tttctgctgc cattcgctgc
    15061 agacgtccag ccgtggtcac gcaacacgtg gccattattc cgctcgcatc caaacttctt
    15121 actgccgtcc agcaccatgt catttgcgca gccgccaggc gggccgtgcg gcaggatatt
    15181 cccctcacct ttgtgggtga agccgtccgc aggtggttta ttgaagaaag cggaattccc
    15241 gctgaaatga cccatatgac ccccgcgggc attgaaccgg ataaatactt ccttgtgccg
    15301 gattccgagc gcgcagcgct gatgcggaaa tggaatctgc cggagaatca gttcaaggtt
    15361 ttgttcgtcg gacgttttta tgagaaaaaa gggctgcagc tgatccggaa ggttgcagag
    15421 cagtgccctg atatcctctt ttccttccgc ggaagcggcc cggatgatcc cgaagcatgg
    15481 aagctgccca atgttcgcat tcttccaatg gtcacagatg aggagcttcg cgagatttat
    15541 ggctgccacg atttgttcat catgccctcg atcggcgaag gctggccggc cgtgatcccg
    15601 caggccatgg cctgcggcct gggctgcatg atctccgaag aaacatttca gggttacaac
    15661 cgcgaccgag accggtttat tgtcgtcccc cgcagtgctg atgctattgt gaagcaactg
    15721 aatgatgcaa aggaggacag gattccctgg ctgaaaaacc gggtcgggat ttcggactat
    15781 gctgtccgca catggaactg gcagcagact gcccgaatct attcagagat tttcgagaaa
    15841 caggtgcaaa acgcagggcg gtcagacgga cggcaggctg atctgcacga gtgaccccgc
    15901 gttgcgctgc ggaacaacgg tccactcctt gagcgcggcc ggaatcagga gggtggttcc
    15961 ttcttcaata tcctctgttc ctccagtcca cctgatttgc agcgggcctt cctctgcaaa
    16021 gagaatatga aaactccggc catcattccg gaaagatttt gagtcattca gatgatgcgt
    16081 cctgaatatg aaatatttgc attggaattc cccggcatcc acgcggggat cttcatcatc
    16141 gttcggccga agcacattca gagcctgttc cagatgcagt tcccgcggcc ggccccagtc
    16201 gtaaatacga taggtcgtat tggaattctg ctggatttcc agcagcaggc agccggcatc
    16261 aatcgcgtgg atccgtcccc cggggacata aactgcggtt ccctttccca ttggaaccgt
    16321 cttaaggcag tccgccacgg catgattctc gatcgcggat cgaagggttt caggcgacac
    16381 cccctttttg agaccggcat agaccttagc acccggtcct gcctccagcg cataccacat
    16441 ctccgttttt gcctcgccgc cgaaccgggc ggcgccctcg tcatccggat gcacctgcac
    16501 actgagacat tcatgagcat ctatgatttt aaccagcagc gggaagcttt cgtcacggcg
    16561 gcctgctccc agaaggactt ctcccatcgt ctgtgtcagc tgatgcagtg tcttcccctt
    16621 cagccgtccg ttggccacaa cactcatgcc atccggacgg tccgcaatct cccatgactc
    16681 ggcatatatt cccgggggca gccctcgcct gtatttgcgc acgatccggt ctcctcccca
    16741 gacatagtcc cgatagaccg gctcaaaccg caaaggataa atctcatgca tcataattca
    16801 ccctcgttcc ggtcaagcac ttcgcgcagc cgcacagcca gactgaagcg ggaatacggt
    16861 ttggtcagta tgcacttaac ctgatccgaa ggaatacccg ctcttacggc catgtccgtg
    16921 ctgaaccccg tcgtgaacaa gactctgagc cccggatgtt ttatgcgcag ccgggcgatg
    16981 atttcaggac cgtttgcctt tggcatgatg acatcgcaga ataccagatg aatctgagcg
    17041 ggatcctgat ccagaatgaa ttccgcttcg gcctcagttt ccgcctcgag ggcctgatac
    17101 cccagcgaag ccagaagtcg tgaagtcaaa tgccggacgg tgtcgtcatc ctccaggaca
    17161 agcacacatt catttccgcg cggcgccggc cgagcttccg gcacttcccg atgctctgcg
    17221 cgcaagtgac tgcaggggaa atacagacga aattcagatc cttttcccgg ctgactgtcc
    17281 agcaccactg ccccacccag ctgccgcatg aaatcggcaa ccatggcaag gcccagtccc
    17341 gatccttctc cctgcttctt cgtcgtgaaa aacggttcaa aggcgcgtgt gcgaacctcg
    17401 tcaggcatgc cgcaacccgt gtccttgacg gaaagaaaaa cgcacagtcc tcccggctcc
    17461 gcctccctga ggggtgtatc agaacaggga caggatccgg tttcaaccgt aagaaaccct
    17521 tcatcgggca tggcatcgcg tgcgtttacg gcaagattga gtatgatctg ttcaagcatg
    17581 gaggagtcag caagcacgct ttcccccagc gcgcttttac ggatatgcag ctggatgcgc
    17641 tctcccagcg tccgctgcat aaacggcgcc atttcatcca caatttcatt caggtcaagc
    17701 ggctggatat ccggcaaacg cgagcggccg atggaaagca gtttttgcgt caggcgggcg
    17761 gcacggcagc actcctgata cacctgagcc atatcctgct gtacaggtcc gtcttccggg
    17821 aggccggtca ttgccagctg actgtagccc atgatggtcg tcagcagatt gttgaagtca
    17881 tgcgatattc cgcagacaag ctgtcccagt gcatccattt tctgagactg cacaagacgg
    17941 gaacgacttt cctgcagagc cgtttcagcc tctttcctct ccagaatttc cgcacggagc
    18001 tggcggttgg ccttgagcag ttcggcggtt ctttcctgca ccctgcgctc gagattgtct
    18061 cgggcgtgct gcagttccat ttcaacatgc ttgcgttctg tgatcttgtg cagtgtggca
    18121 ataacagggc tgtcgggttc atcctgcagc aagcgggcat tcagcgaaca gtattcaatg
    18181 gccccggcct tgtgaatgac cgtaacttcc gcatcctcca gaataccgtc ctgcctcagt
    18241 gccgtcagca gaaccgcaac ctgttccggc tgcacacaga tcgcctggaa aggccggttc
    18301 ttcagctctt ccggctcaaa gccggtgaga gcggcgaccg aaggactcac ggcccgtacg
    18361 cggccgtcaa ggtccagttc gaaatagaca tcctgaatgc tctcgaaaat cctccggtag
    18421 ccgatctgac tctgcctgag ggcttcttcc gtttcccgat agttcgtgac atccagcatc
    18481 acgccataca catagcgggg ctctccgttc tcgtcgtcca tatacgcggc atgattccgg
    18541 aaccatttga tgcggccatc ccgcgcccgg acacgataga ccaggaaaaa agagcccccc
    18601 gttgcattct tgcgatatac ctcattcgtg attcgcgcgc ggtcttcatc caccagctgg
    18661 cgtatccaca ggtgcggatc cgccatccat tccgccggag tgaaacccag aatgctttcg
    18721 acctgaggac tgatataaac cgtgcgattg accggaccaa gctcaacaat atagctgatg
    18781 gccggaagct gttccaccag cttgcggtac tgtcccagat tgagcagaaa atccttagtc
    18841 atggcgtgaa ctctgatcca agaaaatagc accgacggca caaaaaaagc aacatcttcc
    18901 cctcgcacgg cgcgccggcc ggccggctgc cggtgctttt atcgcacctt gatatggatc
    18961 agcgcgcaca caccggccgc aatcgccatc agaacaccgg tggttttatt gaaggcataa
    19021 aaattgtcag cggggtcggg atatttggtc agccagtgct cggaagggaa ccatccgtcc
    19081 ttgataaacc aggcagccag caccaccagc acaacggatg caatcaaata agagcgggtt
    19141 cccttcacat gataattacg cgccatttcc gtttcctctt cttttttaga cagtcatggt
    19201 atcgcatatc ggcgcgggat caagcctgac ctgctcgttc tgctccagcc gccttcttga
    19261 gttcagccac ttccgcctgg gccaggggcc gccactgccc cgaccgcagc gcatcgagct
    19321 tgagcggccc gatccggatc cggcacaagc gttccacttt tttccctgcg gccgcataca
    19381 ttctgcggat ctgccggttc tttccctcca gcagcgtgac ttcggcgcag ttccggtcat
    19441 caggagccag cctcacagcc gccgcggtca ggcactctcc atcgtgctgg atccccgcca
    19501 gaaatttctg cacatcctca gccgtcaaag gggcatcgct ccagaccctg taaaccttgg
    19561 gaatg
//
LOCUS       DAOQWR010000035        19179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3233_length_19179_cov_5.666283, whole genome shotgun sequence.
ACCESSION   DAOQWR010000035 DAOQWR010000000
VERSION     DAOQWR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19179
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3233_length_19179_cov_5.666283"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKK36_04415"
     CDS             <1..673
                     /locus_tag="PKK36_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNR93838.1"
                     /translation="MAKGAGMIEPGMATMLSFILTDAAVEADALQACLLAAVKKSFNL
                     VSVDGDRSTNDTVLCMAGGAAGNKPLSPAHRDWKVFSDALYRVAFDLAMKIIGDGEGA
                     RKVVRIEVKGALNDAEADIAARAVANSMLVKTSWVKDSPNWGRVMDALGYSRAKVDET
                     LVDIDYDEHPAVRGGMASGSSVAELKKIVARECFVIKIDLHLGNGRAEVYSCDCTEEY
                     VRINW"
     gene            683..1555
                     /gene="argB"
                     /locus_tag="PKK36_04420"
     CDS             683..1555
                     /gene="argB"
                     /locus_tag="PKK36_04420"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNR93839.1"
                     /translation="MRAVIEKASVLIEALPYIQRFNGETVVVKFGGSIMEDAEGVKNI
                     LRDIAFMACVGMRPVIVHGGGKSISAEMEKRGLRPSFVQGLRVTDQASIDVVEHVLNH
                     EVNPQLVSVLLAYDCKARGIHGDDILKVRKHTAVDEKTGQKLDWGFVGTVTGADVAPV
                     EAFLHSGITPVITPLGRDEKRHIYNVNADEAAAAVAVALKARKLVFLSDVPGVLSDKD
                     DETTIISSIKAGDIEDLVKRGIISGGMLPKISGAVKAMQAGVRKVHLIDAMLPHSLLL
                     ELFTDKGVGTEIVS"
     gene            1552..2766
                     /locus_tag="PKK36_04425"
     CDS             1552..2766
                     /locus_tag="PKK36_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNR93840.1"
                     /translation="MSTREEIQNLYQQYVMTTYASASLALVRGKGAYVWDSEGKRYLD
                     FLAGISVMNVGHCHPKVVKALRKQAGQLIHVSNLYHNENQPRLAQALVERSLKGGKCF
                     FCNSGAEANEGLIKLARLWGSGQGRFEVITMRNSFHGRTLATLTATGQEKVQKGFHPL
                     PEGFVYADFNNLDSVRSAISGKTAAVLVEAVQGEGGIMPATDEFMKGLRALCDEKNIL
                     LLVDEVQAGMGRTGTWFAWQHYGIEPDAFSLAKALGSGYPLGAVVAGPKVADVFQPGH
                     HASTFGGTPLACAAGLAVVETIEEEGLLENVRKMGNLFRSELEKLVPKYKWIQCVRGR
                     GLMIGLVLNCPAKDLQMSMQEQGLLALATANTIIRFLPPLNIDEKIVKRAASIIRKAC
                     RSFDKQAGVNKQ"
     gene            2763..3950
                     /locus_tag="PKK36_04430"
     CDS             2763..3950
                     /locus_tag="PKK36_04430"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNR93841.1"
                     /translation="MKVVVAYSGGLDTSVQLKWIKEKYNAEIIAFTADVGQEEELSGI
                     EEKAMSTGADKVYVDDLRSEFAKDFVFPMIRAGGVYESGYLLGTAIARPLIAKRMIEI
                     VRENKADAIAHGATGKGNDQVRFELTAYSMEPEIRVIAPWREWEFKGRTDLMNYAKQH
                     NIPVPVTVKKPYSMDRNLLHISYEGGILEDPWSQPPKDMFVLSVDPEDAPDQAEEVEI
                     DFEKGDAVAVNGKKMSPLDVMLTLNKMGAKHGVGRIDMVENRYVGMKSRGVYETPGGT
                     ILYRAREAVEQICMDREVLFQRNTLVPQYAKLVYNGFWFAPERRMLQAAMDDAASTVT
                     GTARVKLFKGSCTVTGRKAKKSLYDPTIASFERENVYNQADADGFIKLNALRLRVRAR
                     AGL"
     gene            3981..5231
                     /gene="lysA"
                     /locus_tag="PKK36_04435"
     CDS             3981..5231
                     /gene="lysA"
                     /locus_tag="PKK36_04435"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNR93842.1"
                     /translation="MEAFEYRKGEMFVDRVPVSRLAERYGTPLYVYNRSYLLGQYRQL
                     VTAFSEINPQIYFAIKANSSAAVISTFAREGAGADVVSGGELWRAIRAGVEPSKIVFA
                     GVGKTVEEIDYALMEGIHCFTVESEPELERISQRAQVLNRPGRIAVRVNPDVDPKTHK
                     YTSTGKKESKFGVDLERAMKAYELAASLPGLEIAGIHMHIGSPIMTVEPYAQALEKVA
                     PVCRELKKKYPKFTQLDIGGGLGIRYRDEQEPFDVQGFAGVVIRYAKELGMSVGMEPG
                     RFLVGNAGLLVCRVQYIKDNPFKKFIISDGGMNDLIRPALYEAYHKILPVKETADTVL
                     GDLVGPVCESGDFLAAERALPKVKQDDLIAVRSAGAYAFAMSSSYNSRPRPAEVMVQG
                     RKVKLIRAREKREDLVRGEKNPEW"
     gene            complement(5327..5926)
                     /locus_tag="PKK36_04440"
     CDS             complement(5327..5926)
                     /locus_tag="PKK36_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93843.1"
                     /translation="MKIYKTAAIIAAALAAQGVLAQPRMGEEMHRGPMQPGKPGGLRG
                     GECIGMLMRVSHDADLQKQAGITDDEAAKLKELATEVQRNMIDLKAAKDKAELDLSTV
                     LESANPSRDAAMAAAEAVSAAQSALLKANIETRLEVIELLGADKVDALQKACPDKRME
                     MRRDGRDSRERKWNKKTRKGPGAECLPGGDIDDDEKDED"
     gene            complement(6049..7098)
                     /locus_tag="PKK36_04445"
     CDS             complement(6049..7098)
                     /locus_tag="PKK36_04445"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HNR93844.1"
                     /translation="MSNNIAFFDTKPYDRQSFDSVNRGYGASITYFDAHLDINTVNLT
                     PGFNAVCAFVNDTIDAAVIDQLVENGVRLIALRCAGFNNVDFKAAFRRIHVVRVPAYS
                     PHAVAEHALALIMSLNRKTHKAYSRTRDGNFSINGLLGFDMHGKTAGIIGTGKIGRCM
                     IEILLGFGMKVLAFDKHRDPSLAFASSANFQYAELKDIYAKSDIISLHCPLTPETEHM
                     INARTIEQMKPGVMIINTGRGKLIQTAALIHGLKTKKIGAAGLDVYEEESDYFFEDKS
                     TEILTDDMLARLLTFPNVLITSHQAFFTAEALRGIAETTLQNVKEFFDGGYLQNEICY
                     QCGGTCLKKEKKRCF"
     gene            complement(7095..7856)
                     /locus_tag="PKK36_04450"
     CDS             complement(7095..7856)
                     /locus_tag="PKK36_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93845.1"
                     /translation="MAFHYRARAVQTTEDPQKAETAPRSSRQAAPAADSGPAGAAGPS
                     PLQDAGLADRVRELETQLGSSANLIASLQASQTSRWSREDRPRRDRNDWMEELKATDP
                     ERYQEIMERRAEARRRMSETFAVKAAHFLDRDTSLMSRVEEENYHHMISLLDETWRIA
                     EKLNTDTPREERWPLMRQMHENIEVLSPLLDAQREREFRQIALDFGYDDMEAAQFVEY
                     MNKIVDVTTMRSFFESMRAGGPPGSGGRRGSGGPR"
     gene            complement(7925..9151)
                     /locus_tag="PKK36_04455"
     CDS             complement(7925..9151)
                     /locus_tag="PKK36_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNR93846.1"
                     /translation="MSSDNTGPIYEALMRISLALNSQLDLSDVLNQVIYYTRLLTGAH
                     NASIVLWNAKKKMFERGASTTEVGDTVAVRVRRTSGATRWIIDHAKPFVVPDTAHDPF
                     IANRMLPENSIRAYAGVPITRESEVLGVLYALSYQNRQFDLDELKWMQELAGLAAIAI
                     SNARMLKSLQELSAFKDAMRRMAAHDLRQPLSIATLYLEMAMIERGTLSDEQFSSLQT
                     AAEALNRMETMIKDLLDYERLTAEGDVVRQPVNLNDIVVQAVSNFAAHAAQKSQQLKT
                     ETAPRAPIVNGDPVLLLEAVGNLIANAVKYTQNGGRILVSLSSDEESETLSVRDNGPG
                     ISPSDMQNLFQPFRRRKEKGKDHGTGLGLSLVKVITEQHGGEVSAESTPGQGSTFSIH
                     IPREAGLIRNRAGGPA"
     gene            complement(9178..9993)
                     /locus_tag="PKK36_04460"
     CDS             complement(9178..9993)
                     /locus_tag="PKK36_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR93847.1"
                     /translation="MSEPAQYLDSHMHLDIVHHCHPDRIQWLKDNGCAVVSWAFSPRE
                     GETAELARYLKFQAETIQSINEQGLPCRFLAGIHPRNIPGDMDPAQCTEFLGPFLAHP
                     LCLGIGEIGLETGGAREIEFFTAQLAAARDAGKNCRTGVHTPRKNKPEITSTVLDVLD
                     CFHELHGRLVVDHCTPETIESVLDHGWMAGITLSPVKSSLADLQAIFKVRPDCAARIM
                     CNTDSGMDFYEDLLISNDCFKRSLETRRALFGGNAADFFNWRPGPCLNAASEA"
     gene            complement(9990..11120)
                     /locus_tag="PKK36_04465"
     CDS             complement(9990..11120)
                     /locus_tag="PKK36_04465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNR93848.1"
                     /translation="MRKSGNPDEWKIQPLAEAALPVYSADPAAKISLCSPSIEIMPGG
                     RIIAAFSQTGPGLRDLPGKKARHPRTGHWMQGRILVSNDFGKTWQDTHLFPFCGGRLF
                     RDGHILYLIGESGNLQIIKSADGGLTWSDPSSLTAHSDAEGDFAEPPANIFVDERHIA
                     MVWMRHTSPGVKGTAPQAAVFMRAARGANLMSPRQWTFGESPAGQERFAGHDAHVVRV
                     TDPHHSLNSTETGTCHIILRPSQGHQGSVMAVKVSSQEDGSLKMEAGAALPLPGCHSR
                     FQILFDPDTKLYWLISAQARTSFKTRRTEEPLLLQLHFSSGLSDWVFAGLLQTAETAL
                     QEVCGAIHGSTLYLAGTSGGKGPHRSSVGLAAVKYFRELVYP"
     gene            complement(11117..12382)
                     /locus_tag="PKK36_04470"
     CDS             complement(11117..12382)
                     /locus_tag="PKK36_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNR93849.1"
                     /translation="MQASSIFRQGVLGIQMLFVAFGALVLVPLLTGLDPSIALLTAGA
                     GTLLFHALTGGQIPIFLASSFAFIAPIQFAVREWGTGAALGGLFCAGLVYLLFSLIVR
                     LGGKDMINRYLPPIVTGPVIMVIGLKLAPVAVCMARSLDGSGTFSRKAILIAACSLLT
                     AVSTVMYGKKLLSLIPVLCGIIVGYIISLLLGVVDFTPVREAVWFAAPWAEALKNGTY
                     ALPRFEIPAITYMLPVALAPAIEHVGDILAISSVTGKNYLQQPGLHRTLLGDGLATSL
                     AALLGGPPNTTYSEVTGAVALTKSFDPLLMRIAAVTAIILAFAGKLGAVLRTIPSPVM
                     GGIMLLLFGMIAAIGINALVRARVDMSRSRNLVIASVILTTGIGDLTVTCGRFCLGGI
                     GLAGLVGVLLNIILPGTGDAHAEHAEPAP"
     gene            complement(12464..14695)
                     /locus_tag="PKK36_04475"
     CDS             complement(12464..14695)
                     /locus_tag="PKK36_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNR93850.1"
                     /translation="MKPNHKRAGKISRPIRKPWQGARSPVEDLVEAVMNTDMDSSRVS
                     PGMRMLANKALQFSSPKTRVVILGGGTGLSTVVGGNAQRADWARMPFVGLKEEFPNLK
                     VVVCTTDDGGSTGRLLKILPMIGIGDLRKSCVSLIRSENLQSTYRISREESLNLVQSI
                     QRIFNYRFPEKAADARRLADPLLVLPVRQREAVPGKLAAALRSLGARAPVKPASHCLG
                     NLLMTSAVFQAAKGRTDRPPGMPEIQKGLDAVARLIGVTPGSLLPATSTPGQLKFRYA
                     NGVEVLGQSKSASARRNFPVERLTAVFSGSPVVSKEVLQSIREADMIVIAPGSVYTSI
                     IPVLQIAQIAETIRANRKALKILGANFWIQEGETDISSRTGKQEFCVSELIEAYDHNV
                     PGGTRDLFQLVLSANLELMPGNILRNYALEGKRPIHLDRARVTAMGFQPVEATLFSPD
                     QLKQAHVIHHDAGKFALALRTILFARKTLHWSDTEKGSSKSPGAAPNRRKSPHSPLPC
                     RYMSEIERTLSSRMFRPASLKKVLLDLAWDNRDILPEHLKYFRAAKVISRKAWHRSTD
                     WDNVLGFYDPEDQCLKVHEELLAKPARLREDLLIALGESLLGKYIAGRSMREGRAGLY
                     PGARLYEIRLRPVNQRHSYLNDEQLRTYLNLARMTPDPRDPLCWHLYLNSTEGFLPPG
                     LLFGLMYAWYLDNACGRIMEYEMSLLRWPPRALLPHQAKERARKQALVNFFRSEIFRH
                     HEK"
     gene            14799..15689
                     /locus_tag="PKK36_04480"
     CDS             14799..15689
                     /locus_tag="PKK36_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93851.1"
                     /translation="MNIFRECGPQTGAQLLERTQMEALPLWQLVRRTPGVRFELAGRR
                     YLRLDREVAGYARLSPSIRREFLTYTVLGLEDQAEVIRARAQMLRQEIRRISRTKFNF
                     ARETVVSIVQSLPRWDVLNERVCFIIAGDITYDMAHAVPRPETSTGRMVRGSDLDIIA
                     VAEDTVPQEFLKALDDAIYERKHFLLMHPDYQEEIDYIVKNLSKVREQLAFDTFENMI
                     AGKILDEGQMLYGSTAVFESVKNLMRELGVSEKINHLEQQASSNRLEAESALLRAGPD
                     CFGSAYYNLFYTREEGDEIY"
     gene            complement(15690..16718)
                     /locus_tag="PKK36_04485"
     CDS             complement(15690..16718)
                     /locus_tag="PKK36_04485"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNR93852.1"
                     /translation="MPFQVIVTRDFDHMSEVAAGLVAEDIRQVVARKSSYALGLATGS
                     SPTGLYKHLAKAANAGAFDASAVTSFNLDEYVGLPGENAQQRALHKESYSCFMIQELF
                     GLLQKKFRETNVPWGTLIDQDRFAAEMKAHPSDWSLQGADKGKAVKIGTDAKSEYLRW
                     IQKEILDAYAAKIKKQGGIDLHIVGVGGRGHVGFHEAGIPFENNPMLLVKLDDNTIAN
                     AVTDGHFATREESPLYAVSMGALLIYRARTVLLLANGKRKTDAVADALLMEPDCSVPI
                     SYGHTLSENGGRMIFVLDREAAAGILDHADELRKRGIGLEDISSQKASVRVADLKFSR
                     NPETGLMG"
     gene            complement(16738..18090)
                     /gene="glmM"
                     /locus_tag="PKK36_04490"
     CDS             complement(16738..18090)
                     /gene="glmM"
                     /locus_tag="PKK36_04490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNR93853.1"
                     /translation="MERMFGTDGVRGVANVHPMTAEMALQIGRATAYVCKKHKGRRHT
                     IVIGKDTRISCYMLESALTAGICSMGVDVLLVGPMSTPGIAFITRSMRADAGLVISAS
                     HNPYQDNGIKIFSRDGFKLPDAEEDEIERLITTDHIKDIRPTAGEIGKAKRIDDAMGR
                     YIVFCKNTFPESMTLEGMKIAMDCANGATYKAAPTIFSELGAEVTAIHCEPNGLNIND
                     RCGSQHTQNLSARVREIKADIGIAFDGDGDRFIAVDETGEEITGDHIMAICGKMYKEL
                     GLLKNNLLISTVMSNFGFFIALKELGIESGASKVGDRYVLEMMQKKGAVLGGEASGHL
                     IFLNHHTTGDGIISALQLLSAMRHYSQPLSKLAKIMKMAPQKMINVEVKSKPPIEKIP
                     EIQEGIKKAEAELKDKGRVLIRYSGTQSMCRVMVEGPTQEMTDRLTAMLVDVVKKSIG
                     "
     gene            18364..>19179
                     /locus_tag="PKK36_04495"
     CDS             18364..>19179
                     /locus_tag="PKK36_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR93854.1"
                     /translation="MSAEMKKKYVYFYGFGKKNTEGNSSMKAILGGKGANLAEMANAN
                     LPVPPGFTITTEACAYYSKHGNKYPPGLKEEIRAQLGKLEKKMGKKLGDARNPLLVSV
                     RSGAAVSMPGMMDTVLNLGLNDKSVLGFIQQTGNARTAWDCYRRFVDMFGDVVMGPSS
                     GLTHHDFEVEMERIKKKYKAKQDTDLTADQLKELVDAYKKVYQKKVKKPFPQDPMVQL
                     ECAINAVFGSWNSERAEKYRTINKITGLLGTAVNICSMVFGNMGDDSGTGVAFT"
BASE COUNT         3840 a         5362 c         5901 g         4076 t
ORIGIN      
        1 catggccaag ggcgcgggca tgattgagcc cggcatggcc acaatgctgt cgttcatcct
       61 gactgatgcg gccgtggagg ctgatgccct gcaggcctgc ctgctggccg cggtgaagaa
      121 gagtttcaac ctggtcagcg tggacggcga ccgcagcacc aatgacactg tgctctgcat
      181 ggccggcggt gcggcaggga acaaaccgct ttcaccggcg cacagggact ggaaggtttt
      241 ttccgatgcg ctttacaggg tcgcgttcga tctggctatg aaaatcatcg gcgacggcga
      301 aggagcccgc aaggtggtca gaattgaagt gaagggtgcg ctcaatgatg ccgaggcgga
      361 tatcgccgcc cgggccgtgg caaattcaat gctggtcaag acctcgtggg tcaaggattc
      421 cccgaactgg ggccgtgtga tggatgcgct tggctattcg cgggcaaaag tggacgagac
      481 tctcgtggac attgactatg acgaacaccc cgccgtgcgc ggcgggatgg cgtcgggaag
      541 ttccgtggcg gagctgaaga aaatcgtcgc cagggagtgt tttgtgataa agatcgatct
      601 gcatcttgga aacggccggg cggaagtcta ctcttgcgac tgcacggaag agtacgtccg
      661 gattaactgg taaagggaag ttatgcgcgc ggtgattgaa aaagcatcag tgctgatcga
      721 ggctctgcct tacattcagc ggttcaatgg cgagacggtc gtcgttaagt tcggcggaag
      781 catcatggag gatgcggaag gcgtgaagaa catccttcgc gacatcgcct tcatggcttg
      841 tgtcggcatg cggcccgtca ttgtccacgg cggcgggaag tccatttccg ccgagatgga
      901 aaagcgcggc ctgcggccct cgtttgtcca gggtctgcgc gtgaccgacc aggcttccat
      961 tgatgtcgtg gagcatgtgc tgaaccacga ggtgaatcct cagctggttt ctgtcctgct
     1021 tgcctatgac tgcaaggcgc gcggcattca cggggacgac atactcaagg tccgaaagca
     1081 cacggctgtg gatgaaaaga ccggccagaa gctggactgg ggctttgtcg gcacggtgac
     1141 cggcgcggat gtcgcgcccg tggaggcatt tctgcactcc ggcatcaccc ctgtgatcac
     1201 gccgctcggc cgggacgaaa agaggcatat ctacaatgtt aatgcggacg aagcggctgc
     1261 ggcggttgcc gttgcgctca aggcccgtaa actggttttt ctttccgatg tgccgggggt
     1321 attaagcgac aaggacgatg aaaccaccat catatcttcc atcaaggcgg gcgatatcga
     1381 agatctggtg aagcgcggca tcatctcggg cggcatgctt ccgaaaattt ccggcgcggt
     1441 caaggccatg caggccggcg tgagaaaagt gcacctgatt gacgccatgc tcccgcattc
     1501 gctcctgctg gagcttttca cggataaggg cgttgggacg gagattgtgt catgagcaca
     1561 agagaagaaa ttcagaatct ttatcagcag tatgtaatga cgacctatgc ttctgccagc
     1621 ctggcgctgg tcagggggaa gggcgcatat gtatgggatt ccgaagggaa gcgctatctg
     1681 gatttcctcg cggggatttc ggtgatgaac gtggggcact gtcatccgaa ggtggtgaag
     1741 gccctccgga agcaggcggg acagcttatt catgtatcca atctttatca caatgaaaac
     1801 cagccgcggc tggcgcaggc gctggttgag cgctcgctca agggcggcaa gtgcttcttc
     1861 tgcaattccg gagccgaggc caacgaggga ctgatcaagc tcgcgcgcct gtggggctcc
     1921 gggcagggcc ggtttgaagt catcaccatg cggaactcgt tccacggccg gacgctggcc
     1981 acgctgacgg ccaccggcca ggagaaggtg cagaagggct ttcatccgct gccagagggt
     2041 tttgtgtatg ccgattttaa caatctggat tctgtccgga gcgcaatttc gggaaaaacc
     2101 gccgcggtgc ttgtcgaggc ggtgcagggc gagggcggca tcatgccggc gacggatgag
     2161 ttcatgaagg gcctgcgcgc gctctgcgac gagaagaata tacttcttct ggttgacgag
     2221 gtgcaggcgg gcatggggcg gaccggaacc tggttcgcgt ggcagcacta cggcattgag
     2281 ccggacgcgt tttcactggc caaagcactg ggaagcggat atccgctggg cgccgtggtg
     2341 gccggcccga aagttgcgga tgtcttccag cccggccatc atgcctcgac ctttggcggc
     2401 acccctctgg cctgcgcggc cggccttgcg gtggtggaaa ccatcgaaga ggagggcctg
     2461 ctcgaaaatg tcagaaagat ggggaatctg tttcggagcg agctggagaa actggttccg
     2521 aaatacaaat ggatacagtg cgtgcgcggc cgcggcctga tgatcgggct ggtgctgaac
     2581 tgtccggcga aagatctgca gatgtccatg caggaacagg ggctgctcgc gctggcgacg
     2641 gcaaatacca tcattcggtt tctgccgccc ttgaatattg acgaaaaaat cgttaaaagg
     2701 gccgcttcca tcattcggaa ggcttgcagg agttttgata aacaagcagg agtgaataaa
     2761 caatgaaagt ggtagtcgca tattcaggag ggctcgacac ctccgttcag ttgaagtgga
     2821 tcaaggaaaa atacaacgcc gagatcatcg ccttcacggc cgatgtcggc caggaagagg
     2881 agctttcggg cattgaagag aaggcgatgt ccaccggggc cgacaaggtc tatgtggatg
     2941 acctgcgttc cgaatttgcg aaggatttcg tgttcccgat gatccgcgcc ggcggcgttt
     3001 atgagagcgg ctatctgctg ggcaccgcca ttgcccgccc gctgattgcc aagcgcatga
     3061 tcgaaatcgt tcgcgagaac aaggccgatg ccatcgcgca cggcgccacc ggaaagggca
     3121 acgatcaagt ccgctttgag ctgaccgcct attcaatgga gccggagatc agggtgatcg
     3181 ctccctggcg tgaatgggag ttcaagggcc ggaccgactt gatgaattat gcgaagcagc
     3241 ataacatccc ggtgcccgtg acggtaaaga agccttacag catggaccgc aacctgctcc
     3301 atatcagtta cgagggcggg attcttgaag atccgtggag ccagccgccg aaggacatgt
     3361 ttgtgctttc tgttgatcct gaagacgctc cggatcaggc ggaagaggtg gagattgatt
     3421 tcgaaaaagg ggatgccgtg gccgtcaacg gcaaaaagat gtctccgctg gacgtgatgc
     3481 tcaccctgaa caagatgggc gcaaaacacg gcgtgggccg gattgacatg gttgaaaacc
     3541 gctatgtcgg catgaagagc cgcggtgttt atgaaacgcc cggcggcacg atcctctacc
     3601 gcgcccgcga ggcggtggag cagatttgca tggaccgcga agtgctcttc cagcgcaaca
     3661 cactggttcc gcagtacgcg aaactggtct acaacggttt ctggttcgcg cccgagcgca
     3721 ggatgcttca ggcggcgatg gatgatgccg cctcaacagt aacaggcacc gcgcgcgtaa
     3781 aattgttcaa gggcagctgc acggtaacgg gccgcaaggc gaagaaatcg ctttacgatc
     3841 cgaccattgc ctcgtttgaa cgcgagaacg tctacaacca ggccgacgcg gacggtttca
     3901 tcaagctgaa tgcgctgcgc ctgcgcgtcc gcgcccgcgc gggtctctga aaccgccaat
     3961 gaacgcgaat ggacgcgaat atggaagcat ttgaatatcg aaaaggtgaa atgtttgtgg
     4021 acagggtgcc ggtgtcaagg ctggccgaaa ggtacgggac gccgctttat gtctacaacc
     4081 ggagttattt gctcggtcag taccggcagc tcgtgacggc cttcagtgag atcaatccgc
     4141 agatctattt tgcgatcaag gccaacagca gcgcggcggt catcagcacg ttcgcccgcg
     4201 aaggcgcggg cgcggatgtg gtttcgggcg gcgaactctg gcgcgcgatt cgtgccggcg
     4261 tggagccgtc caaaattgtt tttgcgggtg ttggcaagac ggtcgaggaa atagattatg
     4321 cgctgatgga gggaatccac tgctttacgg tggaatccga gcctgaactg gaacgcatat
     4381 cgcagcgggc gcaggttttg aaccggcccg gccgcattgc cgtccgcgtg aatcccgatg
     4441 tggatccgaa gacccacaaa tacaccagca ccggcaagaa ggagagcaag ttcggagtgg
     4501 acctggaacg ggcgatgaag gcttatgaac tggccgcctc gctgccgggc cttgagatcg
     4561 cgggcatcca catgcacatc ggctcgccga ttatgaccgt tgaaccctat gcgcaagcgc
     4621 tggaaaaggt cgcccctgtt tgccgcgagc tgaagaaaaa gtatccgaag ttcactcagc
     4681 tcgatattgg cggagggctg ggcattcggt accgcgatga gcaggagcct tttgatgttc
     4741 agggctttgc cggggttgtt attcgttacg cgaaggaact gggcatgtct gtcggcatgg
     4801 agccgggacg tttccttgtt ggcaacgcgg gtttactggt ctgccgggtg cagtatatca
     4861 aggacaaccc gttcaagaaa ttcatcatca gcgacggggg aatgaatgac ctgatccgcc
     4921 cggcgcttta tgaggcctat cacaaaattc tgccggtgaa ggaaaccgcg gatactgttc
     4981 tgggtgatct tgtggggccc gtatgtgaat cgggcgattt tctagcggct gaacgggctc
     5041 ttccgaaggt gaagcaggac gatctgatcg cggtcaggag cgccggcgcc tatgcctttg
     5101 ccatgtcctc aagctacaac agccggccgc gcccggcgga agtgatggtt cagggccgca
     5161 aggtgaagct gattcgcgcc cgcgaaaaac gtgaagacct cgtgcgcggc gaaaaaaatc
     5221 cggaatggta aattgcgcga tggggtgttc cgctccgtcg gaatgattga ataaaaaaag
     5281 aggccgggat ttcccggcct ctttttgtca cgcaggctga acttgatcag tcttcatcct
     5341 tttcatcatc atctatatcg ccgccgggga ggcattcggc cccggggcct ttgcgggtct
     5401 ttttgttcca ctttctctcg cggctgtccc ggccgtcgcg gcgcatttcc atgcgcttgt
     5461 ccggacaggc tttctgcagg gcgtccactt tgtcggcgcc cagcagttcg atcacctcga
     5521 gccgggtttc gatgttcgct ttcaagaggg ccgactgtgc ggccgagacg gcctccgccg
     5581 ccgccatggc ggcatcacgg ctgggattag cgctctccag caccgtgctc agatccagct
     5641 ccgccttgtc tttggcggct ttcagatcaa tcatattacg ctgaacctct gtggccagtt
     5701 ccttcagttt ggcggcctca tcatcggtga tgccggcctg cttctgaaga tccgcgtcat
     5761 gggagacccg catcagcatg ccgatgcact cgccgccgcg caatcccccc ggtttccccg
     5821 gctgcatggg gccccgatgc atttcttccc ccatccgggg ctgcgccaga acgccctgcg
     5881 cggcaagtgc cgctgcgatt attgctgctg tcttgtatat cttcattgct caatcctttc
     5941 gttggttcac gactgtgatg ttcacaatct tcccgtataa cggaagccct ccccaattat
     6001 tgcacggatt cggcggctct aattgtttcc cgaattccct ggccggattc agaagcagcg
     6061 ctttttctcc tttttcaggc aggtcccgcc gcactggtag catatctcgt tctggagata
     6121 tccgccgtca aagaattcct tcacgttttg cagggtggtt tcggcgatgc cgcgcagcgc
     6181 ctcggcggtg aagaaggcct gatgcgatgt aatcagcacg ttcggaaagg tcagcagccg
     6241 cgccagcatg tcatccgtga ggatttccgt tgatttgtcc tcaaagaagt agtcgctttc
     6301 ctcttcgtag acatccaggc cggcggcgcc gattttcttt gttttgagcc cgtgaatcag
     6361 tgcggcggtc tgaatgagtt tgcctcggcc ggtgttgatg atcatgactc cgggtttcat
     6421 ctgttcgatg gtccgcgcgt tgatcatgtg ttcggtttcc ggcgtcagcg gacagtggag
     6481 cgaaatgatg tcggatttgg catagatatc cttcagctcg gcatactgga agttcgcgga
     6541 tgaggcgaag gcaagggagg ggtcccggtg tttgtcgaag gccagcacct tcatgccgaa
     6601 acccagcagg atttcaatca tgcagcggcc gatttttccc gtgccgatga tgcccgcggt
     6661 tttgccgtgc atgtcaaaac ccagcaggcc gttgatcgag aaatttccgt cgcgcgtgcg
     6721 ggagtaggcc ttgtgcgttt tccggttgag cgacatgatc agcgcaagag catgctccgc
     6781 gaccgcatgc ggagaatagg ccgggaccct gaccacatga atgcgccgga aagccgcctt
     6841 gaagtccaca ttgttgaagc cggcgcagcg cagggcgatg agccgcaccc cgttttcgac
     6901 cagctgatct atgaccgccg cgtcaatggt gtcattcaca aaggcgcaaa ccgcgttgaa
     6961 accgggggtg agattcaccg tgttgatgtc gaggtgcgcg tcgaaatatg tgatgctggc
     7021 gccatacccg cggttaaccg agtcgaacga ctggcggtcg tagggcttgg tgtcgaaaaa
     7081 agcgatatta ttactcatcg ggggcctcct gatcctctgc ggccgccgct gcccggcggc
     7141 ccgccggcac gcatgctttc gaaaaacgac cgcatggtgg tgacgtctac gattttgttc
     7201 atgtactcga caaactgagc ggcctccatg tcgtcataac cgaaatcgag ggcgatttgc
     7261 cggaactcgc gttcgcgctg cgcatcgagc agcggggaaa ggacttcaat gttctcgtgc
     7321 atttgccgca tcagcggcca gcgctcctcg cggggggtgt cggtattgag cttttcagcg
     7381 atgcgccatg tttcatccag cagggaaatc atatgatgat agttttcctc ttccaccctg
     7441 ctcatgaggg atgtgtcgcg atcaagaaag tgcgcggcct tgacggcaaa ggtttcgctc
     7501 atcctgcgcc gggcctccgc gcgccgctcc attatttcct gatagcgctc cgggtccgtc
     7561 gccttcagtt cctccatcca gtcattccgg tctcggcggg gccggtcctc gcgggaccac
     7621 cggctggttt gagacgcctg cagcgaggca atcaggttcg cgctgcttcc cagctgtgtt
     7681 tcgagttcac ggaccctgtc cgcaagcccg gcatcctgaa gtggagaagg gccggccgcg
     7741 cccgctggtc cggagtcggc cgcgggcgcg gcttgacggc tggaccgggg tgctgtttcc
     7801 gctttctgcg gatcttcagt tgtttgcacc gcccgcgccc gataatgaaa ggccatgcct
     7861 gtgcctcctg cggcccaggc gaagagcagc gtcaagatgc ctgcctggat gacatggggg
     7921 gctttcaagc cggacctccc gcacggtttc ggatcagtcc ggcttccctg ggaatatgga
     7981 tgctgaaagt ggagccctgt ccgggagtgc tttccgcgga cacctccccg ccatgctgtt
     8041 cggtgatgac ttttaccagg ctcaaaccca gccccgttcc gtggtccttc cctttctctt
     8101 ttcttcgccg gaaaggctga aagagattct gcatgtcaga cggactgatc ccgggaccat
     8161 tatcgcgcac ggacagtgtt tcggattcct cgtcagagga gagggacaca aggatgcggc
     8221 ctccgttctg agtgtatttg actgcgtttg cgatcaggtt cccgacggct tcgagcagca
     8281 gcaccgggtc tccgttcacg atcggggccc gcggggccgt ttcagtcttc aactgctgtg
     8341 atttttgcgc ggcgtgcgcc gcgaaattgg agacggcctg cacaacaata tcgttcaggt
     8401 tcacaggctg ccggacgacg tccccctcgg ccgtgagccg ctcgtagtcg agaagatcct
     8461 tgatcatcgt ttccattcga ttgagcgcct cagccgcggt ttgaaggctg ctgaactgct
     8521 cgtcagaaag cgtgcctcgt tcgatcatgg ccatttccag atagagcgtg gcgattgaca
     8581 gcggctgccg aagatcatgc gcagccatgc gccgcatggc gtccttgaac gcgctcagtt
     8641 cctgaagaga tttcagcatg cgcgcattgc tgatggcgat ggccgccagc cccgccagtt
     8701 cctgcatcca tttcagttca tccagatcga actgccggtt ctgataggaa agcgcataca
     8761 gaacgcctaa aacctcgctc tcgcgtgtaa tcggcacccc cgcataggcg cgaatgctgt
     8821 tttccgggag cattcggttc gcaataaacg gatcgtgcgc ggtgtccgga accacaaaag
     8881 gtttggcgtg atcaataatc cagcgggtcg cgccgcttgt ccggcgcacg cggacggcaa
     8941 cggtatcacc aacctccgtg gtggatgcgc cgcgctcgaa catcttcttc ttcgcgttcc
     9001 agagcacaat gcttgcgttg tgcgcgcccg tcaaaagccg cgtgtagtag attacctggt
     9061 tcagtacgtc gcttaaatca agctgcgaat tcagcgcaag gctgattcgc atcagggcct
     9121 cgtatatcgg gccggtattg tcggatgaca attctttctc catggatacg gcttatatca
     9181 tgcctcagag gcggcattca agcacggccc cggccgccag ttgaagaaat cggctgcgtt
     9241 gccgccgaag agcgcccgcc gggtttcgag actgcgcttg aagcagtcgt ttgagatgag
     9301 gaggtcttcg taaaaatcca ttcctgaatc ggtgttgcac atgatgcggg cggcgcagtc
     9361 cggccggacc ttgaaaatgg cctgcagatc cgcaagcgac gatttaaccg ggctcagggt
     9421 gatccccgcc atccagccgt gatcaagaac gctttcgata gtctccggcg tgcagtgatc
     9481 caccaccagc cgcccgtgca gttcgtggaa acagtccagc acatccagaa cagtgcttgt
     9541 gatttccggt ttgttttttc ggggagtgtg tacccccgtc cggcagtttt tcccggcgtc
     9601 gcgcgcggca gccagctgcg cagtgaaaaa ctcgatttcc cgggcccctc cggtttcgag
     9661 tccgatttcc ccgatgccca ggcagagggg gtgtgcgaga aacggcccga gaaattcggt
     9721 acactgggcc ggatccatgt cgccggggat gttgcgcgga tgaatccccg cgagaaagcg
     9781 gcagggcagg ccttgttcgt taatggactg gattgtctcc gcctgaaact tcaaatagcg
     9841 cgcaagttcc gccgtttctc cttcgcgggg cgagaacgcc cacgagacaa ccgcgcagcc
     9901 gttgtctttc agccattgga tgcgatctgg atgacagtga tgcacaatgt cgagatgcat
     9961 atgactgtcc agatactgcg ccggttcgct catggataga ccagttcccg gaaatatttc
    10021 acagcggcaa gcccgacgga actccgatga ggacctttgc ctccggaggt tcctgcaaga
    10081 tagagcgtgc tgccgtgtat tgcgccgcag acctcctgca gcgcagtttc cgcggtttgc
    10141 aggagtccgg caaagaccca gtcggaaaga ccggatgaga aatgaagctg gagaagcagg
    10201 ggttcctccg tccggcgtgt tttaaagctg gttcgcgcct gcgcgctgat cagccagtaa
    10261 agttttgtgt cgggatcgaa caggatttga aagcgggagt gacatcccgg caggggaaga
    10321 gcggccccgg cctccatttt caggcttccg tcctcctgtg aggacacttt tactgccatg
    10381 acgcttccct gatgaccctg cgagggccgg aggatgatat gacaggttcc tgtttccgtg
    10441 ctgttcaggc tgtgatgggg atcggtcacg cgcacgacgt gcgcatcatg ccccgcgaat
    10501 ctttcctgac cggccgggga ttcaccgaaa gtccactgcc gcgggctcat caggttggct
    10561 ccgcgggccg cccgcatgaa tactgcggcc tgcggagcgg ttcctttgac accgggactc
    10621 gtgtgcctca tccacaccat ggcgatgtgg cgctcatcga caaatatatt cgcgggcggt
    10681 tccgcaaaat ccccctccgc atcggaatgg gcggtgaggg acgatggatc gctccacgtc
    10741 aggccgccgt cggcagactt gatgatctgg agatttccgc tctcgccgat gagataaaga
    10801 atgtgtccgt cgcggaaaag gcgtcctccg caaaagggga acagatgggt atcctgccag
    10861 gtcttcccga agtcgttgga caccaggatg cgcccctgca tccagtggcc ggtgcgcgga
    10921 tgccgcgctt ttttcccggg aagatcccga aggcccgggc cggtctggct gaaggccgcg
    10981 atgattcttc cgcccggcat tatttcgatg gaggggctgc acagcgatat cttcgccgcg
    11041 gggtcagcgg aatagaccgg cagcgcggct tccgcgagcg gctgaatttt ccattcatcc
    11101 ggatttccgg acttcctcat ggtgccggct ccgcatgttc agcatgcgcg tcgccggtgc
    11161 cgggcagaat gatgttcaag agcaccccga cgaggccggc gagtccaatg ccgcccaggc
    11221 agaaccgtcc gcaggtgacg gtcaggtcgc cgatgccggt cgtaagaatc acggaggcaa
    11281 tcaccagatt gcgcgagcgc gacatgtcca cccgcgcccg cacgagtgcg ttgatgccaa
    11341 tcgcggcaat catgccgaag agcagcagca tgatgccgcc cattacggga gaggggatcg
    11401 tgcgcagcac cgcgcccagc ttgccggcga aggcgagaat gatggccgtg actgcggcaa
    11461 ttcgcatcag aagcgggtca aaggatttgg tgagggccac agcgccggtc acttcggaat
    11521 aggtggtgtt gggagggccg cccagcagtg ccgccagcga ggtcgccagc ccgtcgccga
    11581 gaagggttcg gtgcagtcca ggctgctgca gatagttttt cccggtgacc gaggaaatgg
    11641 ccagaatatc gccgacatgc tcaatggcgg gcgccagcgc gacgggcagc atgtaggtta
    11701 tggcgggaat ctcaaaccgg ggcagggcat aggttccgtt tttcagagcc tccgcccatg
    11761 gcgccgcgaa ccagaccgcc tcccgaacgg gggtgaaatc caccacgccc agcagcagcg
    11821 agatgatata gccgacaatg atgccgcaca gcacggggat caggctgagg agtttcttcc
    11881 cgtacatcac ggtggaaacg gccgtgagca gggagcaggc ggcgatgagg atggccttgc
    11941 ggctgaacgt tcccgaaccg tccaggctgc gcgccatgca gacggccacc ggcgcgagct
    12001 tgaggccgat gaccataatc accggaccgg tgacaatggg cggaagatag cggttgatca
    12061 tgtccttgcc ccccagccgc acgatcaggc tgaacagcaa atacaccagg ccggcgcaga
    12121 acagtccgcc cagcgcggca cccgtgcccc attccctgac ggcgaactgg atcggcgcaa
    12181 tgaacgcgaa ggagcttgca agaaaaatgg ggatttgtcc gccggtgaga gcgtgaaaaa
    12241 gaagtgtgcc ggcgcctgcc gtcaagaggg cgatggatgg atcaagtcct gtcagcagcg
    12301 gcacgagcac gagggctccg aaggcgacaa aaagcatttg gattcccagc acgccctgtc
    12361 tgaagatgga tgaagcctgc atctttctct cctttcaaat gaccgcagcc attctagcgt
    12421 ccttcgcctt cggaacaagg tttgaaacac gggaaaagtg ccgttacttt tcgtgatggc
    12481 ggaagatttc actgcggaag aagttcacca gcgcctgttt tcgcgcgcgc tccttggcct
    12541 ggtgcgggag cagggcgcgc gggggccatc gcaggaggga catttcatat tccatgattc
    12601 tgccgcaggc gttgtctaga taccatgcgt acatcaggcc gaacagcagc cccggcggaa
    12661 ggaatccttc ggtgctgttc agatacaaat gccagcagag gggatcgcgc ggatcggggg
    12721 tcatgcgggc cagattcaga taggtccgca gctgttcgtc gttcaggtac gaatgccgct
    12781 ggtttacggg ccggagacgg atttcataca gccttgcccc gggatacagg ccggcccggc
    12841 cttcgcgcat gctgcggcct gcaatatact tcccgagcag cgattccccg agggcaatca
    12901 gcaggtcctc gcgaaggcgg gcgggtttgg cgagcagctc ctcatgcacc ttgaggcact
    12961 ggtcttcggg atcatagaag ccgagaacat tatcccagtc ggtgctgcgg tgccacgcct
    13021 tccgggagat gacctttgcg gcccggaaat atttcaggtg ttccggaaga atgtcgcggt
    13081 tgtcccacgc cagatccaga agcaccttct tcagggatgc ggggcgaaac atcctgctgc
    13141 tcaaagtccg ctcaatctct gacatataac ggcagggcag ggggctgtgc ggcgattttc
    13201 tccggttcgg agcggccccc ggcgacttgg agcttccttt ttccgtgtca ctccagtgga
    13261 gggtcttccg ggcgaaaagg atggtccgca gggccagcgc gaactttccg gcgtcatgat
    13321 gaatgacatg cgcctgcttc agctggtcgg gcgaaaacag agtggcctcg accggctgga
    13381 aacccatggc cgtcacgcgc gcgcgatcca gatggatcgg gcgttttcct tcgagggcat
    13441 aattgcggag gatgttgccg ggcatgagtt ccagattggc gctcagcaca agctggaaca
    13501 ggtcccgggt tccgcccggc acattgtgat cataggcctc aatcagttcg gaaacgcaaa
    13561 actcctgctt ccccgtgcgg ctggaaatat ccgtttcgcc ttcctgtatc cagaaattgg
    13621 cgccgaggat tttcagcgcc tttcggttgg cccggatggt ttccgcgatc tgcgcgatct
    13681 gcagaacggg gatgatgctg gtgtatacac tgccgggcgc gatgacaatc atgtctgctt
    13741 cccggatgga ctgaagcacc tctttgctca caacgggtga accgctgaac actgccgtga
    13801 ggcgttcgac gggaaaattc cttcgggcgg aggccgattt gctctgtccg agcacttcaa
    13861 ccccgtttgc ataacggaac ttaagctggc ccggtgtgga tgtggcggga agcaggcttc
    13921 ccggcgtcac gccgatcagc cgtgcgacgg catccagccc cttttgtatt tcaggcattc
    13981 caggcggccg gtccgtgcgg ccttttgccg cctgaaatac ggcggaagtc atcaggagat
    14041 ttcccaggca gtgactggcg ggtttgaccg gtgcccgggc gccaagtgat ctcagcgctg
    14101 cggccagttt cccgggtacg gcttcccgct gcctgaccgg aagaaccagc aggggatcag
    14161 ccagccgccg cgcatcagcg gctttctcgg gaaagcgata gttgaatatc cgctgaatgg
    14221 actgtaccag attgagggat tcttcgcggc tgatgcggta ggtgctctgc agattctcgg
    14281 agcggatcag cgaaacgcag gatttccgca ggtcgccaat gccgatcatc ggcaggattt
    14341 tcagcaggcg tccggtggag ccgccgtcat ccgttgtgca gaccaccacc ttgaggttgg
    14401 ggaattcctc tttcagtccc acaaacggca ttcgcgccca gtctgcgcgc tgggcgttcc
    14461 ctccgaccac ggttgagagg cccgtccctc cccccagaat gaccacccgg gttttgggac
    14521 tgctgaactg cagggccttg ttcgcaagca ttcgcatccc cggcgaaacg cgcgaagaat
    14581 ccatgtcggt gttcatcaca gcctcgacca aatcttcaac gggactccgg gctccctgcc
    14641 agggcttgcg gatgggtctt gaaattttcc ctgcgcgttt atgatttggc ttcatttatg
    14701 ttccggatta ttgcttcaat gtccgcagac tatcaggaag tgaaatccat ttcatagaaa
    14761 aatatgcggg gaccgttgtt atgacgccgc aggatgttgt gaacatattc agggagtgcg
    14821 gaccgcaaac cggcgcgcag ctgctggagc gcacgcagat ggaggcgctg ccgctctggc
    14881 agcttgtccg gcggacgccc ggtgtgcgct ttgagctggc ggggcggagg tatctccggc
    14941 tggaccgcga agtggcgggc tatgcccggc tttctccgtc cattcgcagg gaattcctga
    15001 cctacactgt tctcggtctg gaggatcagg ccgaagtcat cagggcgcgg gcgcagatgc
    15061 tgagacagga aatccgccgc atcagccgga ccaagttcaa ctttgcgagg gagacggtgg
    15121 tgtcaatcgt ccagtcactt ccccggtggg atgtattgaa tgaacgggtt tgtttcatca
    15181 ttgccggaga catcacttat gacatggcgc acgcggttcc ccgtcccgaa accagcaccg
    15241 gaagaatggt gcggggatcg gacctggaca tcatcgccgt tgcggaggac acggtccctc
    15301 aggagttcct gaaagcgctg gacgatgcca tctatgagcg caagcatttc ctgctgatgc
    15361 atccggacta tcaggaggaa atagactaca tcgttaaaaa tctttccaaa gtccgcgagc
    15421 agctcgcctt tgacaccttc gaaaacatga tcgcgggaaa gattctggat gaggggcaga
    15481 tgctctacgg gagcacggcg gtgtttgagt cggtcaaaaa tttaatgcgg gagcttggcg
    15541 tatctgaaaa gataaatcat cttgaacagc aggcttccag caaccgtctg gaagcggaat
    15601 ctgcgctcct ccgtgccggc ccggactgtt tcggcagcgc gtactacaat ctcttctaca
    15661 cacgcgaaga gggcgacgaa atatactgat cagcccatca ggccggtttc gggatttctg
    15721 gagaatttca ggtcggccac gcgcacagag gctttttgtg agctgatatc ttccagtcca
    15781 atgccgcgtt tccggagttc atcggcgtga tccagaatcc cggccgctgc ctcgcggtcc
    15841 agcacaaaga tcatccgccc gccgttttcc gacagggtgt ggccgtacga aatgggcacg
    15901 gagcagtccg gctccatcag cagcgcgtcg gccacggcgt ccgtcttgcg ctttccgttg
    15961 gcaagcagca gcacggtgcg cgccctgtag atcagcaaag cacccatgga taccgcataa
    16021 agaggactct cctcccgggt ggcaaaatgg ccgtcggtta ccgcattggc gatggtgttg
    16081 tcatccagct tgacgagcag catcggattg ttttcaaagg gaatgccggc ctcgtgaaac
    16141 ccgacgtggc cgcggcctcc gacgccgacg atgtgaagat caatgccgcc ctgctttttg
    16201 attttggccg cataggcatc cagaatttcc ttctgaatcc agcgcagata ttcggatttt
    16261 gcatccgtgc caatcttcac ggccttgccc ttgtcggctc cctgcagaga ccagtcggag
    16321 ggatgggctt tcatttcagc cgcgaagcgg tcctggtcaa tcagcgtgcc ccagggcaca
    16381 tttgtttccc ggaacttttt ctgcagcagg ccgaaaagct cctgaatcat gaagcagctg
    16441 taactctcct tgtgaagggc gcgctgctgg gcgttttcgc cgggcagccc gacatattca
    16501 tcgagattga agctggtgac tgcagaggcg tcgaacgcgc cggcgttggc ggcctttgcc
    16561 agatgcttgt agaggccggt tggcgaactg ccggtggcca gtcccagcgc ataagatgat
    16621 ttccttgcaa caacctggcg aatgtcttct gcaacaaggc cggcggcgac ttcgctcatg
    16681 tgatcgaagt cgcgggtgac aataacctga aaaggcatga tgaattccct ccgcggttca
    16741 gccaatgctc tttttgacga catccacgag catggcggtc agccggtcgg tcatttcctg
    16801 cgtggggccc tcgaccatca cacggcacat ggactgtgtg ccggaatagc ggatgagcac
    16861 gcgccccttg tccttgagtt ccgcttcagc ttttttgatt ccttcctgaa tttcgggaat
    16921 cttctcgatc ggcggcttgg atttgacttc cacgttgatc attttctgcg gggccatttt
    16981 catgattttt gccagtttgg acagaggctg gctgtagtgc cgcatggccg agaggagctg
    17041 gagcgcggaa ataattccgt cgccggtggt gtggtgattc aggaaaatca ggtgacccga
    17101 ggcctcgccg ccgagcacgg cgcctttttt ctgcatcatt tccagcacat agcggtcgcc
    17161 caccttgctc gcgcccgatt caatgcccag ttccttcaag gcgatgaaga atccgaaatt
    17221 gctcatcacc gtagaaatca gaaggttgtt tttgagcagg cccagttcct tgtacatctt
    17281 cccgcagatg gccatgatgt ggtcgccggt gatctcctcc ccggtttcgt ccacggcgat
    17341 gaagcggtcc ccgtcgccgt cgaacgcgat gccgatatcg gccttgatct cgcgcaccct
    17401 ggcgctcagg ttctgtgtgt gctgcgatcc gcagcggtca ttgatgttga gaccgttcgg
    17461 ctcgcagtgg atggcggtca cctccgcgcc gagttcggaa aagatggtgg gcgcggcctt
    17521 gtaggtggcg ccgttggcgc agtccatggc aatcttcatt ccctccagcg tcatgctttc
    17581 gggaaatgtg ttcttgcaga acacaatgta gcgccccatg gcgtcgtcaa tgcgcttggc
    17641 cttgccgatc tctccggcgg tagggcggat atccttgatg tggtcggtgg tgatcaggcg
    17701 ttcgatctcg tcctcctctg cgtcgggcag cttgaagccg tcgcgcgaga aaatcttgat
    17761 gccgttgtcc tggtaggggt tgtgggaggc ggaaatcacc aggcccgcgt cggcgcgcat
    17821 gctgcgggta atgaaggcga tgccgggcgt gctcatgggg ccgaccagca ggacatccac
    17881 gcccatcgag cagatgccgg ccgtgagggc ggattccagc atatagcagc tgatgcgcgt
    17941 gtccttgccg atcacaatcg tgtgccggcg gcccttgtgc ttcttgcaga cataggcggt
    18001 tgcgcggccg atctgcaggg ccatctcggc ggtcatgggg tgaacatttg cgacgcctcg
    18061 cacaccatcg gttccaaaca tgcgttccat gtattcctct cctttcaatc aaattcagaa
    18121 gcgaatctac aatcacctgc cgcgaaaaaa cagagaatct ttcactgaaa ataaaaaagt
    18181 gcttccgcaa ccccggcggc gcagcggccc cgccgggttg tccttcgtat tccgggcgca
    18241 tggatgggct gtttttcggt tggcgcttga ctctgggatg gggtctgtta gcatgcgcgc
    18301 ggtttaagag tgacgtgatt caagcgtttg aaccctgata aacagacata ggagttgtct
    18361 agcatgagtg cagagatgaa gaagaagtat gtgtatttct acggcttcgg gaaaaagaac
    18421 accgaaggca acagttcaat gaaagccatc ctcggcggca agggcgccaa ccttgctgaa
    18481 atggcgaacg cgaatctccc ggtgcccccc ggcttcacga tcacgaccga agcctgcgcc
    18541 tattattcca agcacggcaa caaatatccg cccgggctca aggaggagat ccgggcccag
    18601 ctcggcaagc tcgagaagaa gatgggcaag aagcttggcg atgcccgcaa cccgctcctg
    18661 gtttccgtcc gttccggcgc cgcggtctcc atgcccggca tgatggacac ggttctcaat
    18721 ctgggcctga acgacaagtc cgttctcggg ttcattcagc agaccggcaa tgcccgcacc
    18781 gcgtgggact gctatcgccg ctttgtggac atgttcggcg atgtggtgat gggtccgtcc
    18841 tccgggctga cgcatcacga tttcgaagtc gagatggagc gcatcaagaa gaaatacaag
    18901 gcgaagcagg acaccgatct caccgccgac cagctcaagg agctggtgga cgcctataag
    18961 aaggtctatc agaagaaggt gaagaagccg ttcccgcagg atccgatggt gcagctggaa
    19021 tgcgcgatca atgccgtgtt cggctcgtgg aattcggagc gtgccgagaa ataccgcaca
    19081 atcaacaaga tcaccgggct gctcggcacc gccgtcaaca tctgctcgat ggtgttcggc
    19141 aacatgggcg acgattccgg cacgggcgtg gcgttcacg
//
LOCUS       DAOQWR010000036        19172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3235_length_19172_cov_5.744207, whole genome shotgun sequence.
ACCESSION   DAOQWR010000036 DAOQWR010000000
VERSION     DAOQWR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19172
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_19172_cov_5.744207"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKK36_04500"
     CDS             complement(<1..885)
                     /locus_tag="PKK36_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93855.1"
                     /translation="MQFSWDQFILKARMPALAAVVLAAALFASRHLPLPQNPVPPAEP
                     PPPEWSSPLEIDPATWKVFKSLDGGAGVAAGSLAQRFRLAGTFSVFSDAAGKNQRAIL
                     DDFAKKTQHLVSEGDALDSITVVSIFHDHVVLREDEKEETLWLSFQGSDRPGATPGRP
                     VTVTAAEQGALEENRFGKRVGEKRWVLSRPALLEYYQEILDDPERITSLYASLRPDYN
                     KEGAILGYIYDPQGEKEFFEAMGIREGDTIRKVNSMIMKSQSRAEYMLREFMQNRLNT
                     VVIDFERDGVEDKMIYLIR"
     gene            1029..2114
                     /locus_tag="PKK36_04505"
     CDS             1029..2114
                     /locus_tag="PKK36_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNR93856.1"
                     /translation="MRKIKKSRCMRCFLLLFWLALPGLCRSAETNAASNAAIEEEELY
                     FFYDTWNVIYNKEDLVREIPDSIRIASYNLENFTDGYEDPPERTPKRLAAHVKGAAKI
                     IAELDPDIIILQELENKYALRLLNKALTNAYPLGYTTFFRHRWGEIDTLNIGVLSRIP
                     LKDVRTIRFTKANGADRPIRGMLAFEVEVGPDDHRLLLYGIHLKSNYGNDAANQRQRK
                     RSLEILMTDVAAVASNAPGREYEVLVLGDTNVDPDNEQFKKDVSLKPLSSMSDLWRGR
                     PLEERITIPTRYGDPEMEFPPAAFDRIFVSKNLKKAPWVAGQAQVLQKGCHTNDSSVL
                     PGDKGHVSDHYPVWVDIHRKKPSPASK"
     gene            2202..2822
                     /gene="thrH"
                     /locus_tag="PKK36_04510"
     CDS             2202..2822
                     /gene="thrH"
                     /locus_tag="PKK36_04510"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNR93857.1"
                     /translation="MEIACLDLEGVLVPEIWINVSKRTGIDELKLTTRDISDYNVLMR
                     QRLKILEKHKLGLPDIQAVIEAMGPLEGAHDFLNWLRARYQVIILSDTFYEFAAPLMR
                     QLGYPTLLCHHLEVTEYGFVSDYKLRQKDQKRQAVKAFHKLNFHVIAAGDSYNDTSML
                     AEADAGILFCPPQNVIDEFPQFQVTRNYDELKVAFAAASAAIQKKD"
     gene            2828..5356
                     /gene="leuS"
                     /locus_tag="PKK36_04515"
                     /pseudo
     CDS             2828..5356
                     /gene="leuS"
                     /locus_tag="PKK36_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
     gene            5353..5541
                     /locus_tag="PKK36_04520"
     CDS             5353..5541
                     /locus_tag="PKK36_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93858.1"
                     /translation="MSRKIVQAPGFVRLWFGLTANERLYIAVVLLIALIGLFARHAHL
                     KRERAVPFDPAELEQRGY"
     gene            5543..5962
                     /locus_tag="PKK36_04525"
     CDS             5543..5962
                     /locus_tag="PKK36_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNR93859.1"
                     /translation="MSTDCLFCKIVRGDIPCAKVYEDENFLAFLDIGPIIKGHTLVIP
                     REHYASLPETPAPLLADMMSVVQRIAGAQMNALKADGINIVQNNGRAAGQLVDHIHFH
                     IIPRFTTDGHKWNWAAKSYDSPAEMQSMADQISARCS"
     gene            6007..6432
                     /locus_tag="PKK36_04530"
     CDS             6007..6432
                     /locus_tag="PKK36_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93860.1"
                     /translation="MNKGSFEEALLQLAEENPQYDPHVYGFMREALDFTIKLLKKPAE
                     GEARHVTGRELTDGIRQYALREFGPLAKTVLNHWGIFTTEDFGKVVFLLISKGIFGKS
                     EKDSAADFANGYDFEDAFRTPFRPLAGRAEKFFEQKELF"
     gene            6432..6812
                     /locus_tag="PKK36_04535"
     CDS             6432..6812
                     /locus_tag="PKK36_04535"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNR93861.1"
                     /translation="MENQEKDKASLFSQLVAMFSTSAMHHLGKLVDPGSGKAEVNLDG
                     ARMSIDLLTMLQEKTAGNLTEDEDRMLKEVLTVLRLNYVETANQSPPPASSAPAQDDK
                     PKREKAESAAGKEEKEPRFHKSYS"
     gene            6812..7507
                     /locus_tag="PKK36_04540"
     CDS             6812..7507
                     /locus_tag="PKK36_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93862.1"
                     /translation="MVRLIKWADAAAREVWAFLKFAGHLARPSSHPAVQVSGLLSLAI
                     LIGLLAYISRATVHYAYIPPGESVLQQAELHLLTAVRAEPDAFKPNMRLPHRSEIRSS
                     GAIHLKAFFPEYDLMHVDDPRVWWESDHDTDDVEDDHLVHYAMEIPLKRLIELVHREG
                     GKLKVQDAYRADGIHYPKSLHKEGRAIDLTCENMTLEKLAKLCWAADFDWVFYEAPKG
                     GGHHIHASVRADR"
     gene            7592..8014
                     /locus_tag="PKK36_04545"
                     /pseudo
     CDS             7592..8014
                     /locus_tag="PKK36_04545"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
     gene            8198..8893
                     /gene="deoC"
                     /locus_tag="PKK36_04550"
     CDS             8198..8893
                     /gene="deoC"
                     /locus_tag="PKK36_04550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNR93863.1"
                     /translation="MIDHTILKPETSRSDVDRICKEALQYKFCSVCVNPVNVPQVAKA
                     LKGSTVKVCCVVGFPLGAADPSIKALETRKAIREGAQEIDMVINVGALKSGDYDLVLR
                     DIRAVVEACQERRILSKVILETSLLSDEEKVKACELSMKAGASYVKTSTGFSGGGATA
                     ADIALMAATVASKRLGVKASGGVRNYQDAINMINAGATRIGASSSVNIIEEAKAVAEG
                     REYKPDSKSKGAY"
     gene            8887..9510
                     /locus_tag="PKK36_04555"
     CDS             8887..9510
                     /locus_tag="PKK36_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93864.1"
                     /translation="MLVAELRTYVFLDSLQPQHASFLATVAKGFLPTAGQASLFVEIA
                     PGIAINSVTDVALKSTNVTPGMQIVERQYGMLEIHSDSQADVREAGRAILRELDLKEE
                     DRIKPKVLTSQVIRSVSDYQTQLINRMRHGNMIVPGETMYVMEIAPAGYAALAANEAE
                     KAAHINILEVRATGSFGRVYLGGDEKDIDVAYRAAEQAVEAIVGREG"
     gene            9555..9857
                     /locus_tag="PKK36_04560"
     CDS             9555..9857
                     /locus_tag="PKK36_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNR93865.1"
                     /translation="MAEALGMIETRSFPASVEAADAMVKAAKVELVSFEKTGGGYTSV
                     VVRGDVAAVKAACDAGQIAAGRVGEVVAVHVIARPHANVDAVLPLGRATESSKAKK"
     gene            9918..10214
                     /locus_tag="PKK36_04565"
     CDS             9918..10214
                     /locus_tag="PKK36_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNR93866.1"
                     /translation="MNLGKVIGTVVATRKESSMDGLKFLLVQHLDHERKPTGSQVVAA
                     DAVGAGPGEMVLVATGSSARQTAATDKRPCDAVIMAIVDSWSVDGVTKYCKSDD"
     gene            10337..11782
                     /locus_tag="PKK36_04570"
     CDS             10337..11782
                     /locus_tag="PKK36_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HNR93867.1"
                     /translation="MAKLTDQDIEAIAQKIVSDLKGAAPAPSASVERTPAAAVAGPGV
                     FQTLDEAVAAAGKAQPVFVALPMETRAKIIENIRKSMMENSVALAKAAHEETGLGRFE
                     DKIVKNQLVTMKTPGIEDLPPKAYTGDHGLTLEEPAPFGVIGSITPTTNPTSTIICNT
                     IGMIAAGNSVVFNCHPSAVNCSMQTIVLLNKAIMEAGGPPNVITAVAKPTIESAQALM
                     KHPGIRLLVVTGGGGVVKAAMESGKRAICAGPGNPPVVVDETADLEQAGRDIVLGAST
                     DNNIICADEKEVLVVSSVADKLIQVMTRNNAVLIDKSKLQALEKVIFKEMAGPRGHAT
                     VNRDCIGKNADVILAKMGMNVPSTTRLGIIEVDQNHPLVWTEQMMPILPIVRVPDASY
                     AIDFAVEVEGGCRHTFVMHSRNIDNLSKMAKKCNASLFVKNGRSQTAFGLGGEGYCSF
                     TIASPTGEGMTGPHSFSRSRRCILVDHFRIV"
     gene            11782..12393
                     /locus_tag="PKK36_04575"
     CDS             11782..12393
                     /locus_tag="PKK36_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNR93868.1"
                     /translation="MKKFPAMALVEFNGIAVGMHATDAMLKKSPINLLKCGTISRGHY
                     LTVVAGTTASVDEALDEGLFLAGDNVVDSTFLPDIDTRLHNAILGQSLPMSAGAIAVM
                     ETPTVCCNVRAAEMALKGTPVELVEIRLGDSQMGGRGMSIFQGELHDIEASIDIAVAY
                     LQGKNIEPVYKILTAPHEGMLAQIENSTRFLNSTNLELEGEFD"
     gene            12395..12682
                     /locus_tag="PKK36_04580"
     CDS             12395..12682
                     /locus_tag="PKK36_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNR93869.1"
                     /translation="MKLGKVIGQVVPCEVYEGLETVPMLWVQPLDKKGHPKGAAIVCA
                     DSTRMAGPGELIYYEGGREAAMTLDPVFVPVDHAIVGIVDDMALDQGGGDA"
     gene            12679..12948
                     /locus_tag="PKK36_04585"
     CDS             12679..12948
                     /locus_tag="PKK36_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNR93870.1"
                     /translation="MIAGRVTGHIFSTINHPFYDGKRMLVVEKIDENGKALNDYVIAV
                     DTVDAGPGDKVLIVDEGNGARQILQSSDGPVRSVIVGIIDQITVD"
     gene            complement(12974..16075)
                     /locus_tag="PKK36_04590"
     CDS             complement(12974..16075)
                     /locus_tag="PKK36_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR93871.1"
                     /translation="MLISNYAIKFRTAVFVFIVVLIFAGVSAYLRLPREGAPDLTIPY
                     VFLTAVYEGTAPSEMEKLVTVPLEEQFNDLENVKDITSTSAEGVSSIAIEFLSGEDMD
                     LALQRVKNKIDLARPDLPADLDEPVAQAVNFSTDWPVLLFTLSGDTPLDRLKNLAEDL
                     EDRIEQLPGVKQAVISGTREREIRVEIDISRLAAFGIPMDLVLQRIASENRTISAGNL
                     ETRGDKFQVRIPGEFELAPNIRDLMLVDRGGRSVYLRDIATITDTYKDLSSISRINGK
                     PSVSISLMKRSGENTVGVIDRVRTVLDSYGFPPGIEVTIVMDQSEYVRMMIEELENNI
                     ATGFILVVVVLLVFLGWRNAFFVGLAIPFSMLIAFTVISARGMTLNMIVLFSLVLAVG
                     MLVDNAIVIVENIYRNRTLGLTRHESARRGASEVAWPVITSTLTTLAAFCPLLFWPDI
                     MGQFMGFLPRTLLIVLSASLFVAIVINPAVCSALISARKREARPRQHWFVHGYEKLLR
                     FVLANRLTVLALGFAFLVFSFMLYARKDLGFELFPEVEPRSATIGIKFPEGTSIERTD
                     SAVAAIEEKLKKYEDIKFCLSTVGSAAGGIIGGGQGGTHIANIHVEFHAAQDRKGSTF
                     DLIAQIRDDIGLIPGAEVTVTKQEEGPPMGNPVEIEISGDDFEVLSDYASEILHRIDT
                     VPGLVELRDDMEDALPEIQFVVNRERAALFGLDTSRIGNFLRTAVYGTESSKFRADED
                     EYDITLRLPEEQRNTVTLLDQILIPAPTGQNVPLTSLGRVEYTGGKGAIKRKNQKRVI
                     KIIGDNQHRGVDEIFADVKVRLSDMTLPRGYTITYAGDTQEMQESGEFLGRAFMIASG
                     LIFVILVIQFNSVLLPFVIMFTVLLSLIGVMWGLMLCRMKFGIIMTGLGVISLAGIVV
                     NNAIVLIDCILQRREEGMDTREAVIAAGAMRLRPVLLTAVTTILGLLPMAVGYSVEFH
                     QWPPRIIAGAESTQWWAPMAIAVVFGLLLATILTLLLVPSMYSTVESVASFFRKRYGI
                     KEE"
     gene            complement(16087..17073)
                     /locus_tag="PKK36_04595"
     CDS             complement(16087..17073)
                     /locus_tag="PKK36_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR93872.1"
                     /translation="MAIFKPAAPEPEAPPEKSVAVRTLLIEPQAVPLGITVPARIEAF
                     VEAVLSAEKSGRVVEILAEKGDLVKQGRILMRLDDRTSKAMLEQAEIENRQMAADLKR
                     WQEMEKSGAVSTKESEDIKLRKEASDIAVRQADIGLSQCFVKSPVDGQISSRKVEEGE
                     YANEGQPVFTVVDTSRVKVRFDIPERRILSVGTGTEMRFTGPALGGRTFTGTVSFVSP
                     AANRENNAFPAELLVENAAGDLKPGMVVAVDLPLGVMDNAVAVPLAAVIPKKGEHVVF
                     VVEEGRAVRRLVKLDRILDDRAVLSEGLSAGDQLVIEGHRALSDGVLLDIMN"
     gene            complement(17150..18478)
                     /locus_tag="PKK36_04600"
     CDS             complement(17150..18478)
                     /locus_tag="PKK36_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR93873.1"
                     /translation="MWRNILFCFLFPVLLVAADRPSAEHAYSLEDCIALGLQRSLDLS
                     NARRGELLAVEKVRQVRARALPSLDASSSFTRLDEVPTFPGMEEPFGREDNYAAGVEA
                     SQLLYSGGGVRAALRAARDYRAFASEDTRRQQAELIRDIRQAFYEALYAEKAVEVAEA
                     SVQQLSDFEQQARTRYQNAAASEYDRLTAQVNLANERPRLVQARNRYELVMAALKDLI
                     LADGDDFVIEGELEYRPVDFTYEGLIETAARNRPELRQAQLNVQMREENIRVARSDYR
                     PEIRAFANYKGTNPGDEDPSADDWEWGWNAGLSASWNIMDGGLKKAEFRTAELQRDIA
                     VAEQENLRRAIRLEIRNAFLTLKHAEEVVLSTRETVALAEKALQIARVRYDEGLATHL
                     EFADSNLSLNNAKLYHLMALKSHQQALAALDHACGTDLLEDNVEQAENEK"
     gene            complement(18552..>19172)
                     /locus_tag="PKK36_04605"
     CDS             complement(18552..>19172)
                     /locus_tag="PKK36_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR93874.1"
                     /translation="SYEFYQLIEECGGRFHITGLEHFRDLKEPIVFIGNHMSTLETMV
                     MPFFVASVMEATYVVKERLVTGPFFGPIMRARDPIPVGRTNPREDFKRVMNEGCEKLA
                     RGVSIIIFPQHTRKVEFIPEEFNSIGVKLASRAGVRVIPVALKTDWWGNARWAKAFGP
                     IDRSKPIHFAFGPPISIKSPGGRDENQQIIAFIQSHLRQWEAGGNL"
BASE COUNT         4418 a         5563 c         5414 g         3777 t
ORIGIN      
        1 ccggatgaga taaatcatct tgtcttcgac gccatcgcgc tcaaagtcta tgacgacggt
       61 attgaggcgg ttctgcatga actcccggag catgtattcc gcccgcgact ggctcttcat
      121 gatcatcgag ttgactttgc gaattgtgtc gccctcccgg atgcccatgg cttcaaaaaa
      181 ttccttctcg ccctgcggat catatatata ccccaggatt gcgccttcct tgttgtagtc
      241 gggccgcagc gacgcataca ggctggtgat ccgttccggg tcgtcaagaa tctcctggta
      301 gtattccagc aaagcgggcc ggctcagcac ccagcgcttt tcgccgacac gctttccaaa
      361 cctgttttcc tccagcgcgc cctgctccgc cgccgtcacg gtgacgggcc ggcccggagt
      421 ggctcccggc cggtctgatc cctgaaaact cagccagaga gtctcttctt tttcatcttc
      481 gcgcagaacg acatgatcat gaaaaatcga aaccacagtg atgctgtcaa gggcatcgcc
      541 ttcactcacc aggtgctgcg tcttcttcgc gaagtcgtcc agaatggcgc gctggttttt
      601 cccggccgca tccgagaaaa cggaaaatgt cccggccagc cggaaacgct gagccagtga
      661 cccggcggcg acccccgccc cgccatccag ggatttgaat actttccacg tcgcgggatc
      721 aatctccagc ggggatgacc attccggagg aggcggctct gcgggcggaa caggattctg
      781 cggaagcggc aggtgcctgg aagcaaacag ggccgcggcc agaacaacgg cggcaagggc
      841 cggcatccgc gccttcagaa tgaactgatc ccaggaaaac tgcatgcgag gaccatacct
      901 tttaaggggt ccgatggcaa tctgttagac acttcagatt gatgtcctcg agacggaaca
      961 catccggaga cggcgggatc gaatcaaatg cattgccaaa ttccgttttc ttaagctagg
     1021 attggacgat gaggaagata aagaaatcaa ggtgcatgcg ctgttttctg ctgctgttct
     1081 ggctggcact gccggggctc tgccgcagcg ccgaaacgaa cgcggcctca aatgcggcca
     1141 ttgaggagga ggagctttat ttcttctatg acacatggaa cgtcatttac aacaaggaag
     1201 accttgtcag ggaaataccg gattccattc gcatagcctc atataatctt gagaacttca
     1261 cggatggtta cgaagacccg cccgaacgga cgccgaagcg gctcgcagct cacgtcaagg
     1321 gggccgcaaa aatcattgcc gagcttgatc ccgacatcat catcctgcag gaactcgaaa
     1381 acaaatacgc cctgcggctg ctcaacaagg ctttgaccaa tgcctatccc ctgggataca
     1441 ccactttctt ccggcaccgc tggggcgaaa ttgacacgct caatatcggg gttctatccc
     1501 gtattcccct gaaagacgtg cgcaccatcc gcttcaccaa ggccaacggc gctgaccgcc
     1561 ccattcgcgg catgctggct tttgaagtgg aagtcgggcc cgacgaccac cggctgctgc
     1621 tttacgggat tcacctcaaa tccaactacg gcaacgacgc ggcaaaccag cggcagcgca
     1681 agcgaagcct ggaaatcctg atgacggacg tggcggcggt ggccagcaat gcccccggcc
     1741 gcgagtacga agtgctggtg ctcggcgaca ccaatgtgga tcccgacaat gaacagttca
     1801 aaaaagatgt ttcgctcaag cccctgtcct ccatgtcaga cctgtggcgg ggaagaccgc
     1861 tggaggagcg gatcaccatc cccacccgct atggcgatcc cgaaatggag tttcccccgg
     1921 cggcgttcga ccgcatcttc gtgagcaaaa acctcaagaa agcgccgtgg gtggctggtc
     1981 aggcgcaggt gctccagaag ggctgccaca ccaacgactc ctctgtcctt cccggcgaca
     2041 aggggcatgt atcggatcat taccccgtct gggtggatat ccaccggaaa aagccgtccc
     2101 ctgcctccaa ataaccacgc ggcagccggt ttaggaaatc ccctgcttga acgctcccgg
     2161 ccggttgtgt agagtggcgg gcctgaattg aggttatatt tatggaaatt gcatgtttag
     2221 acctggaagg cgtgctggtg ccggagatct ggatcaatgt gtccaaacga acgggcatag
     2281 acgagctgaa gctcaccacc cgcgatatct ccgattacaa cgtgctgatg cggcagcgcc
     2341 ttaaaatact ggagaagcac aagctggggc tgcccgacat tcaggcggtg attgaggcca
     2401 tgggcccgct ggagggcgct cacgacttcc tgaactggct ccgggcccgc tatcaggtca
     2461 tcattttgtc cgacactttc tatgagtttg ccgcgccgct gatgcgtcag ctcggatacc
     2521 ccacccttct ctgccatcat ctggaggtga ccgagtacgg gtttgtgagc gattacaaac
     2581 tgcgccagaa ggatcagaag cggcaggcgg tgaaggcttt tcacaagctc aatttccatg
     2641 tgattgccgc cggcgattca tacaacgaca ccagcatgct ggccgaggcc gatgccggca
     2701 tccttttctg tccgccgcag aacgtgatcg atgaatttcc ccagttccag gttacaagga
     2761 actatgacga gctgaaggtg gcctttgccg ccgccagcgc agccattcaa aagaaagact
     2821 gattcccatg aacgaaaagt atccgtttta cgagatcgaa cccagatggc agcagtactg
     2881 ggaagaccat aaactgtttg aagtggacac cgcacagtcc tccggcaagt actactgcct
     2941 gatgatgttc ccctatccct ccgggaccct ccatgtgggg tacggccgca actacatcat
     3001 tggcgacgcg gtgacccgtt acaaaaaaat gcaggggctg aatgtgctga cccccatggg
     3061 ctgggacgcc ttcgggcttc cggccgaaaa tgcggccatc tagaccggca cccatcctcg
     3121 cgtcagcaca ctgaacaaca tctcggtgat caaaaaacag ctccgcgact ggggctgctg
     3181 ctacgactgg aaccgcgaag tcgcctcctg cctgccggac tactataaat ggacacagtg
     3241 gctgttcctt aaattctacg aacggggtct ggcctacaag acaaaggcgc cggtcaactg
     3301 gtgcccgtcc tgcgccacag tgctggccaa cgagcaggtt gtggagggca agtgcgagcg
     3361 atgcgaatca gaggtggtgc cgcgcgacct tgaacagtgg ttcttcaaga tcacagacta
     3421 tgcccagcgg cttctggatg acctttccaa gctggacggc tggccggagc gggtcaagac
     3481 catgcagaag aactggatcg gccgcagcga gggcactcag gtgaacttca ggctggtgcc
     3541 gcgcgatgac gggaaaccgg atcaaaagac ggatatcccc tgcttcacga cgcgcgtgga
     3601 caccatctac ggctgcacct acatggtgct ggcccccgaa taccgcgaac tggccgcaat
     3661 gattgaaggc ctgcccgagg aaaaagccgt gcaggcttat gtccggcagg cggaactgac
     3721 ctccaagatt gaccgcgcgg ccgacacgaa cgaaaaaacg ggcgtgttca ccggccgtta
     3781 tgtgatcaat ccctacaacg gcgaaaaagt tcccctgtgg gtcgccgatt atgttcttat
     3841 ggaatacggc accggcgcgg tcatggccgt tccggctcac gacacccgcg actgggcctt
     3901 cgcgaaaaag tacggtctcg aaatccgcat ttcgattcag aatccgaaag gcacactgaa
     3961 gctggcggag atgaaggagg cctatgtcga ggacggcatc acggcggact ccggcccctt
     4021 cacgggccag ccgaatcgcg atgcgatccg ggagatgacc gaatacgctg cgcagaaggg
     4081 cttcggaaaa gccacgatcc attacaagct gcgcgactgg ctgatcagcc gccagcgcta
     4141 ctggggcgcg cccatcccga tgatctactg cgattcctgc ggcatggtgc cggtgccgga
     4201 aaaagatctt cccgtgcttc tgccggacga tgtggagttc cggcctcacg gggaatcccc
     4261 gctgcgcagc agtgcgacct tcatgaacgt gaagtgcccc aaatgcggga tacccgcgcg
     4321 ccgggagagc gacaccatgg acaccttcgt ggattcaagc tggtatttcc ttcgttattt
     4381 gaacgcgcgg gatgaccggc aggccgttga caaaaagatc tgctcggaat ggatgccggt
     4441 ggatcagtac atcgggggaa tcgagcacgc catcctgcac ctgatgtatg cgcgcttctt
     4501 cacaaaggcc atgtgcgata tcggcctgat cacgtttgac gagcccttcg cccatctgtt
     4561 cacacagggc atgatctgca agaaaagcga caaggacgga cagctctaca agatgtccaa
     4621 atccaagggc aatgtggtca gtcccgccga gctgatccgc gaatacggcg ccgacactgt
     4681 gcggctgtat acattattca tcggcccgcc cgaaaaggat gccgaatgga acgatgccgg
     4741 cgtcgaggga gcctaccgct tcctgaagcg catctggaaa aaggtttatg accatcgtga
     4801 cctgctgatt gcggcaaagg cgctggaatc cgacttgaat gccatggaag aaccggagcg
     4861 caatctgtac cgcaagatgc atgagtccat tcagcgcatc acgcgcgacc tggagggggc
     4921 ttttcatttc aacacggcca tcgctcagat catggagctg atgaacgcgc tcgatcaatt
     4981 gtccgtgtca gcggacagtt cggaaaactg ccgcgcagtt ttccgtgaaa cggtggaaac
     5041 cattctgatc ctgatttcgc ctttcgctcc gcatatcgcg gaggaactgt gggcggaact
     5101 gggacatcag ggcgggattc tgaaggccgc gtggccgaaa gtcaacgagc tggccctgca
     5161 gcgcgacgag attgagctgg tcatccaggt caacggcaag ctgcgcggca agataaccgt
     5221 tccggcaaac tccagcagca aggatgttga agccgccgcc ctggcatcgc ctgacgccct
     5281 gcggtggata gagggaaaaa cggtgcgcaa ggtcatcgtt gtccccggga aactgatcaa
     5341 tattgcagca tcatgagcag aaaaatcgtc caggcgccgg ggtttgtacg gctttggttc
     5401 ggcctcacgg ccaatgaaag gctttacatc gcggtggtgc tgctgattgc cctgatcggg
     5461 ctgtttgcgc gccatgcgca cctgaaaaga gaacgggccg tgccgtttga cccggccgaa
     5521 cttgaacaga gaggatactg acatgagcac agactgcctt ttctgcaaaa tagtccgcgg
     5581 ggatatcccc tgtgccaaag tgtatgaaga tgagaacttc ctggcatttc tggatattgg
     5641 tccgatcatc aagggtcaca cgctggtgat tccgcgggag cactatgcct cgctgcctga
     5701 aactcccgcg cccctgctgg cggacatgat gagcgttgtg cagcggatcg cgggcgcgca
     5761 gatgaatgcg ctgaaggcgg acggcatcaa tattgttcag aacaatggcc gcgcggccgg
     5821 acagctggtc gaccacattc attttcacat cattccgcgt ttcaccacag atggccacaa
     5881 atggaactgg gccgccaagt cctatgattc accggcagag atgcaaagca tggcggatca
     5941 aatcagcgcc cgctgttcct gacgggccgg gttgacagaa acgggaattg gagtttaatt
     6001 gacttcatga ataaggggag tttcgaagaa gcgctgctgc agctggcgga ggaaaatccg
     6061 cagtacgacc cccatgtcta cggattcatg cgggaggcgc tggatttcac catcaagctg
     6121 ctcaaaaagc cggccgaagg agaggctcgc catgtcacag gccgggaact gacagacggc
     6181 atcaggcagt acgccctcag ggaattcggc cctcttgcca agactgtgct gaatcactgg
     6241 ggaatattca caacggaaga tttcggaaaa gtcgtctttc tgctcatcag caagggaatt
     6301 tttggaaaga gtgaaaagga ttcggctgcg gatttcgcaa acggctacga ttttgaagac
     6361 gccttcagaa ctcctttcag gccgctggcc ggccgggcgg agaaattctt tgagcaaaag
     6421 gagctgttct aatggaaaat caggaaaagg ataaagcctc tcttttctcg cagcttgtgg
     6481 cgatgttcag cacatcggcc atgcatcatc tcggcaagct ggtggacccc ggctcgggca
     6541 aggccgaagt caatctcgac ggcgcgcgca tgtccattga tctgctgacc atgcttcagg
     6601 agaagacggc cgggaatctg acggaggacg aggaccggat gctgaaggag gtgctgaccg
     6661 ttctccgcct gaactacgtg gaaaccgcaa atcagtcccc cccgcccgct tcgtctgcac
     6721 ccgcgcagga tgataagccg aagagggaaa aagctgaatc cgcggcaggg aaggaagaga
     6781 aagaaccccg cttccacaag tcatacagct gatggtcaga cttatcaaat gggccgatgc
     6841 cgcagccagg gaggtttggg ccttcctgaa gtttgcaggc catcttgccc ggccgtcatc
     6901 gcatccggcc gttcaagtga gcggcctgct gagcctggcc atcctgatcg gccttctggc
     6961 ctatatctcc cgcgccacag tgcactatgc atatatcccg cccggtgaat ctgtgctcca
     7021 acaggcggaa ctgcacctgc tgaccgcggt cagggcggaa ccggatgcct tcaaacccaa
     7081 catgcggctt ccgcatcgaa gcgagataag aagctcggga gccatccatc tgaaggcctt
     7141 cttccccgaa tatgacctga tgcatgtgga tgacccccgc gtctggtggg aaagcgacca
     7201 tgatacagat gacgtggagg atgatcatct ggtgcactac gccatggaaa ttcccttgaa
     7261 gcgcctgatt gagctggtgc atcgtgaagg aggaaaactg aaggttcagg acgcctaccg
     7321 cgcggacggg atccattacc ccaaatcact tcacaaggaa ggcagggcca ttgatttgac
     7381 ctgcgagaac atgactctcg aaaagcttgc caagctttgc tgggccgccg attttgactg
     7441 ggtcttctat gaggctccga agggcggagg tcatcacatc catgcctccg tccgcgccga
     7501 ccggtgaggt atttcccggc ggcctccgta caattatgca ctttacgcaa agcataacgg
     7561 ccgctatctt cattgcatca tgaattcaaa gatggcggtt gcaataggtt cggatcacgg
     7621 cggttttgat ctcaagcagg cgctcattcc atatctcaaa gagatcggtt tcactgtggt
     7681 ggactgcggc atacgcgaca aggccaccgc cgactatccc cgcattgcct tcggagtcgc
     7741 ccagcgtgtg gcctccggcc agtgcgcacg gggcattgtg attgacggcg caggcatcgg
     7801 gtcggccatg accgccaaca aggttcgcgg cgtgcgggct gccgcctgct acagcgttgc
     7861 gctggccaac aatgcccgcg agcacaatga cgcaaatgtc ttgactctcg gctcgggcca
     7921 gaccagtctg gaggacgcta aaggcattat aaccaagttt ctaggcaccg attgcaccgc
     7981 ggcgcggcat ctgcgccggg tgcggatgat agaggaagtc gagcgcggcg cgctgaaagt
     8041 tcagccgccg gaatcaggaa aacaggagaa cgaagtggac ctttcacctc aggatgtcga
     8101 acggattgca caacgggtca aacagctcat ttccggaggc ggaagcgccg cgccttcggg
     8161 caaagcggcg ggagtcaccc ccacccaact cgcgggtatg attgatcata ccattttgaa
     8221 gccggaaacc tcccgcagcg atgttgatcg catttgcaag gaagcgctgc agtacaagtt
     8281 ctgctcggtg tgcgtgaatc cggtgaacgt gccccaggtg gccaaggcgc tcaagggctc
     8341 aaccgtcaag gtctgctgtg tggtcggatt ccccctgggc gcggccgatc cctccatcaa
     8401 ggcccttgaa acccgcaagg ccatccgcga aggcgcgcag gaaattgaca tggtcatcaa
     8461 tgtcggcgcc ctgaaaagcg gagactacga cctggttctg cgcgatatcc gcgccgtggt
     8521 tgaagcctgc caggagcggc gcatcctttc caaagtcatt ctggagacca gcctgctgtc
     8581 cgatgaggag aaggtcaagg cctgcgaact gagcatgaag gccggggcca gttatgtgaa
     8641 aacatccacc ggcttcagcg gcggcggagc cacggctgcg gacatcgcgc tgatggcggc
     8701 gacggtggcc tccaagcggc tgggagtgaa agcctcgggc ggagtccgaa actaccagga
     8761 cgccatcaac atgatcaacg ccggcgccac gcgcatcggt gccagcagca gcgtgaacat
     8821 tattgaagaa gccaaggccg ttgccgaagg cagggaatat aaaccggatt cgaaatcgaa
     8881 aggagcatac tagtggctga attaagaaca tatgtgtttc tggacagtct gcaaccgcag
     8941 cacgcgtcct tccttgcgac cgtggcgaag ggatttttgc cgacggccgg acaggcgtcg
     9001 ctgttcgtgg agattgctcc gggcatcgcg atcaacagcg tgaccgacgt ggccctcaag
     9061 tccacaaacg tgacgccggg catgcagatc gttgaacgcc agtacggcat gctggaaatc
     9121 cattcggact cccaggccga cgtgcgtgag gccggacgcg ccatcctgcg cgaactggac
     9181 ctcaaggaag aggaccgcat caaacccaag gtgctcacaa gtcaggtcat ccggagcgtg
     9241 tccgactatc agacgcagct catcaaccgc atgcgccatg gaaacatgat tgtccccggc
     9301 gaaaccatgt atgtgatgga aatcgccccg gccgggtatg ccgcgctggc ggccaacgag
     9361 gctgaaaaag cggcgcatat caacatcctg gaagtgcgcg caaccggaag cttcgggcgt
     9421 gtatatctgg gcggcgatga gaaggatatt gacgtggcct accgcgcggc ggagcaggct
     9481 gtcgaagcaa tcgtgggaag agaaggttga ttttccccgt catcatcaga aaacaaagaa
     9541 gagagaggta agaaatggct gaagcattag gtatgatcga aacacggtct ttccccgcga
     9601 gcgtggaagc ggccgatgca atggttaagg cagcgaaggt tgaactggtc tcctttgaga
     9661 agaccggcgg aggatatact tccgtcgtgg tgcggggcga cgtggccgct gtgaaagccg
     9721 cctgtgacgc cgggcagatc gccgccggac gcgtgggtga agtggtggcc gtgcatgtga
     9781 ttgcacgccc gcatgccaac gtggacgcgg tccttccgct gggacgcgcc accgagtcgt
     9841 ccaaggccaa gaaataattt agcgcgcaaa gcggccgggc ggtgatgccg cccggccgcc
     9901 ttcaggagtt ccaaggaatg aatcttggca aagtgatagg cacagtggtt gccacgcgca
     9961 aggaatccag catggacggc ttgaagttcc tgctggtgca gcatttggat cacgaacgca
    10021 aacccaccgg ttctcaggtc gtcgcggcgg acgccgtcgg cgccggaccc ggggaaatgg
    10081 ttcttgtcgc aacaggcagt tccgcccgcc agaccgcggc caccgacaaa cgtccctgtg
    10141 acgccgtcat catggccatc gtggacagct ggagcgtgga cggcgtgacc aaatactgca
    10201 agtcagacga ctgatggtca gtcgctgcgc gcgtggatga ttctcccgga cggacctcac
    10261 ggtttccggt tccgcgttga tttgccgccc gccaaagcgg gcaatgtgtt cagttataga
    10321 cgaaagaagg tatgctatgg caaaactgac cgatcaggat attgaagcca ttgcccagaa
    10381 aatagtcagc gatctgaaag gcgcagcccc tgccccgtcc gcatctgtgg aaaggactcc
    10441 ggccgccgcc gtcgccgggc ccggcgtgtt tcaaacactg gatgaagccg tcgccgcagc
    10501 aggcaaagcc cagcccgttt tcgtcgcgct tccgatggaa acgcgcgcaa agatcattga
    10561 aaatatccgc aagagcatga tggagaattc cgtcgccctg gccaaggcgg cacacgagga
    10621 aaccgggctt gggcgctttg aggacaagat tgtcaagaat cagctggtga ccatgaaaac
    10681 ccccgggatt gaagatcttc ctccgaaggc ctataccggg gatcacggac tgacactgga
    10741 ggagccggcc ccgttcggcg ttatcggctc cattactccg acgacaaacc cgacctccac
    10801 catcatctgc aacacaatcg gcatgattgc cgcagggaac agcgtcgtgt tcaactgcca
    10861 cccgagcgcc gtgaactgtt cgatgcagac cattgtcctt ctcaacaagg ccattatgga
    10921 agccggcgga cctcccaatg taatcacggc cgttgccaaa ccgacgatcg aaagtgcaca
    10981 ggcccttatg aagcacccgg gcatccgcct gctggtggtc accggcggcg gcggtgtggt
    11041 aaaggcggcc atggagagcg gcaaacgcgc gatttgcgcc ggacccggca acccgccggt
    11101 tgtcgtggac gaaaccgcgg atctcgaaca ggccggccgc gatattgttc tcggcgcctc
    11161 gactgacaac aacattatct gcgccgatga aaaggaagtg ctggtcgtca gcagcgtggc
    11221 cgacaagctg atccaggtca tgacgcgcaa caatgccgtt ctgatcgaca agagcaagct
    11281 tcaggcgctg gagaaggtca tcttcaagga aatggccggg ccgcgcggcc atgcaaccgt
    11341 caaccgtgac tgcataggga agaatgccga tgtcatcctc gcgaagatgg gcatgaatgt
    11401 gccaagcaca acgcgtctcg gaatcatcga ggtggatcag aaccatccgc tggtctggac
    11461 tgagcagatg atgccgatcc tccctatcgt gcgcgttccc gacgccagct atgcgattga
    11521 tttcgcagtg gaggttgaag gcggatgccg tcacaccttt gtgatgcact cgcgcaacat
    11581 tgacaatctc agcaaaatgg cgaagaagtg caatgccagc ctgtttgtga aaaacggacg
    11641 ctcccagacc gcattcggcc tcggcggcga aggctactgc tcgtttacca tcgccagtcc
    11701 caccggcgaa ggcatgaccg gcccgcacag cttctcgcgt tcgcgccgct gcatccttgt
    11761 ggatcacttc aggattgtgt aatgaagaaa ttcccggcca tggcactggt ggagttcaac
    11821 ggcatcgccg ttggcatgca tgccaccgac gccatgctga agaaatcgcc catcaatctg
    11881 ctgaagtgcg gaaccatcag tcgggggcat tatctcaccg ttgtggccgg aacaacagcc
    11941 tctgtggacg aagcccttga tgaagggctc tttctggccg gagacaacgt ggtggacagc
    12001 acatttctcc cggatattga tacgcgtctg cacaatgcga tactggggca gtcgcttccc
    12061 atgagtgccg gcgccatcgc cgtcatggaa acgcccaccg tctgctgcaa tgtgcgggct
    12121 gccgaaatgg cgcttaaggg cacgccggtc gaactggttg aaatccgcct gggcgactca
    12181 cagatgggcg gacgcggcat gtcaattttt cagggggagc tgcacgatat cgaggcatcc
    12241 atagacattg cggtcgccta tctgcagggc aaaaacatcg agcccgtgta taaaatcctc
    12301 acggccccgc atgaaggaat gcttgcgcag attgaaaaca gcaccaggtt cctcaactca
    12361 accaacctgg aacttgaagg ggagtttgac tgaaatgaaa ctgggcaaag tcattggaca
    12421 ggttgttcca tgcgaggttt acgagggact cgaaaccgtt cccatgctct gggtgcagcc
    12481 gctcgacaag aagggccatc ccaagggcgc agcgatcgtc tgtgcggatt ccacccgcat
    12541 ggcgggtccc ggggagctga tctactatga gggcggacgg gaagccgcaa tgacactgga
    12601 tccggttttt gttcctgttg accatgccat tgtcggcatc gtggacgaca tggcgctgga
    12661 ccagggtgga ggtgacgcat gatcgcaggc agagtgaccg gccatatctt ctccacgatc
    12721 aaccatccct tctatgacgg gaagcgcatg cttgtcgtcg agaagattga cgaaaacggg
    12781 aaggcgctga acgattatgt cattgccgtg gacaccgtgg atgccggacc cggcgacaag
    12841 gttctcatcg tggatgaggg caacggagcg cgccagattc ttcagtcctc cgacggcccc
    12901 gttcgctccg tgattgtcgg gatcattgat cagatcacag tggattgatc agccggtccg
    12961 gcacggctcc ggcctattcc tccttgattc cgtaccgctt ccggaaaaac gaagcaacag
    13021 actctaccgt gctgtacatc gaaggcacaa gcagaagtgt aagaatggtg gccagaagca
    13081 ggccaaacac cacggcaatg gccattggcg cccaccactg ggttgattcg gctccggcga
    13141 taatgcgcgg cggccactga tgaaattcca cactatagcc caccgccatg ggaagcagac
    13201 ccagtatggt agtgaccgcc gtcagcagga cggggcgcag gcgcatggct ccagcggcaa
    13261 ttacagcttc tctcgtatcc atcccctctt cccgccgctg caggatgcag tcaatgagca
    13321 caatggcatt attaacgact attccggcca gactgatcac ccccaggccc gtcatgatga
    13381 tcccgaattt catccggcag agcatcagcc cccacatgac cccaataaga gataatagga
    13441 cggtaaacat gataacaaac gggagcagca cggagttgaa ctggatcacg agtatcacaa
    13501 agatgagtcc gctggcgatc atgaaagctc tgcccagaaa ctcgcccgac tcctgcattt
    13561 cctgcgtatc gccggcgtac gtaatcgtat agccgcgggg aagtgtcata tcggacagcc
    13621 gcaccttcac atcggcaaag atttcatcca ctcccctgtg ctgattatcc ccgatgatct
    13681 tgatcacccg cttctggttc tttcgcttga tggcaccctt cccgcctgtg tattcaacac
    13741 ggcccagaga ggtcaggggc acgttttgtc cggtcggtgc gggtatcaga atctgatcca
    13801 gcagggtcac ggtattacgc tgctcttccg gaaggcggag agtgatgtca tattcatcct
    13861 cgtcggcccg gaatttgctg ctctctgttc catagaccgc cgtgcgcagg aaattaccga
    13921 tccgggaggt gtccagacca aacagcgcgg cgcgctcgcg gtttacgaca aactgaattt
    13981 ccgggagagc atcctccata tcatcgcgca gctccacgag cccggggacc gtatctatcc
    14041 ggtggaggat ttcagaggcg taatccgaaa gcacctcgaa atcatccccc gaaatctcaa
    14101 tttcaacggg attccccatg ggcggacctt cttcctgttt cgtcacggta acctcagccc
    14161 cgggaatgag acctatgtca tcgcgtatct gcgcgatcag atcaaaggtg ctgcctttcc
    14221 ggtcctgcgc cgcatgaaac tccacatgaa tgtttgcaat gtgtgtccct ccctgcccgc
    14281 ctcctataat tccaccggcg gcagatccca ccgtggagag acaaaacttg atatcttcgt
    14341 acttcttcag cttctcttca atagccgcaa ccgctgaatc cgtgcgttca atgctggtgc
    14401 cttcggggaa tttaatgccg atggttgcgg agcggggctc cacttcggga aagagttcaa
    14461 acccgagatc ctttcgcgca tacagcatga atgaaaacac aaggaatgcg aaacccagcg
    14521 ccagcacggt cagtcggttc gcgagcacga accggagcag tttctcatag ccatgaacaa
    14581 accagtgctg ccgcggacga gcctcacgct ttctggcact gatcagcgcc gaacacacag
    14641 ccgggttaat gacgatggcg acgaaaagcg atgcgctcag aacgatcagc agtgtgcgcg
    14701 gcagaaatcc catgaactgc cccataatat ccggccagaa aaggagagga caaaaggcgg
    14761 ccagcgtggt gagcgttgag gtgatcacgg gccaggccac ttcagatgcg ccccgtcggg
    14821 cggattcatg ccgggtcaaa cccagggtcc tgttgcgata gatgttttct acaatcacaa
    14881 tggcgttatc cacaagcatg ccgaccgcca gcaccagact gaacagcaca atcatgttca
    14941 gcgtcatgcc tctggcacta atgaccgtaa aggcaatcag catggagaag ggaatagcca
    15001 ggcccacaaa gaatgcgttg cgccatccca gaaatacaag cagcaccacc acaacaagaa
    15061 taaagccggt ggcaatattg ttttccagtt cttcgatcat catgcggaca tattccgact
    15121 ggtccataac aatggtgacc tcgattcccg gcgggaaacc ataactgtca aggactgtgc
    15181 gcacgcggtc aatgaccccc acggtgtttt cgcctgagcg cttcatcagc gaaatggaca
    15241 cgctgggctt cccgttgatg cgtgaaatgg aggatagatc cttgtaggtg tcggtgatcg
    15301 tggcgatatc gcgcaggtag acggaccggc caccgcgatc gaccagcata aggtcgcgga
    15361 tatttggggc cagttcaaac tcaccgggga tgcggacctg gaatttgtcc cctcgagtct
    15421 ccagattccc ggccgatatg gttctgtttt cagaggctat cctctggagc accaggtcca
    15481 tcggaatccc aaaggcagcc aggcggctga tgtcaatttc cacccggatt tctcgctccc
    15541 gcgtcccgga tatcactgcc tgtttcaccc cgggaagctg ttcgatgcga tcttccaaat
    15601 cctcagcaag gtttttgaga cggtccagag gagtgtcgcc ggacaaggtg aaaagaagta
    15661 caggccagtc tgtgctgaaa ttgacagcct gcgcaaccgg ttcatccagg tcggccggca
    15721 gatcgggacg ggccagatca atcttgttct tcacccgctg gagagcaagg tccatatcct
    15781 ccccggagag gaactcaatg gcaatggaag ataccccctc agccgaggtg gaggtgatgt
    15841 ccttcacgtt ctcaaggtca ttgaactgct cctccagcgg aaccgtaacg agcttctcca
    15901 tttcagacgg agccgtgccc tcgtaaaccg ccgtcaaaaa aacatagggg atcgtgagat
    15961 ccggcgcgcc ttcacgcgga agcctcaaat aggcgctcac gccggcaaaa atcagcacca
    16021 cgatgaatac aaacaccgcg gtacgaaatt taatggcata gttgctgatc agcatcaggt
    16081 gttccttcag ttcataatat caagaagcac cccgtcggac agcgcgcgat ggccctcaat
    16141 caccagctga tctccggcgg acagcccctc ggacaaaaca gcccggtcat ccagaatgcg
    16201 gtccagcttg acaagccgtc gcacggcacg accttcctcc acaacaaaaa ccacatgctc
    16261 ccctttcttg ggaatgaccg ccgccagcgg caccgcaacg gcattgtcca taacgccaag
    16321 cggaagatca acagcgacca ccatgccggg cttcaggtcg cccgccgcgt tctccaccag
    16381 gagttccgcc gggaatgcat tgttttcgcg gtttgctgca ggggaaacaa atgaaaccgt
    16441 ccccgtaaag gtgcgcccgc ccagcgccgg gccggtgaat ctcatctctg ttcccgtccc
    16501 gacggacaaa atgcggcgtt cagggatatc aaaacgcacc ttgacccttg aagtgtccac
    16561 gacagtgaac acaggctggc cctcattggc atactcgccc tcctccacct tacggctgga
    16621 tatttgaccg tccacaggac ttttcacgaa gcactgcgaa agaccaatat ccgcctgtcg
    16681 aactgcaata tcagaggcct ccttgcgaag tttgatatcc tcagattcct tggtggaaac
    16741 tgcgccgctt ttttccattt cctgccagcg ctttaaatcg gcagccatct gccggttctc
    16801 tatttcggcc tgttccagca tggctttcga cgtccggtca tccaggcgca ttaaaatccg
    16861 gccctgtttg acgagatccc ctttttcggc cagaatctca accacccgcc cggatttctc
    16921 cgcggaaaga acggcttcca caaaggcctc aatccgggcc gggaccgtga ttcccaaagg
    16981 aactgcctgc ggctcaatca gcagggtccg aacagcaaca gacttttccg gcggcgcttc
    17041 gggttcaggc gcagcaggtt taaaaatcgc catgagaacg attccgagta caaccagagt
    17101 gagaataatc cacaggatga ttctgccgat tacatgtttc ctggaatttt catttttcat
    17161 tttccgcctg ctccacgttg tcttccagca aatcagtgcc gcaggcatgg tccagtgcgg
    17221 ccagggcctg ctgatggctc ttaagagcca tcaggtgata cagttttgcg ttgtttagtg
    17281 agagattgct gtctgcaaat tccagatgag tggccagccc ctcgtcataa cggacgcggg
    17341 caatctggag ggccttctcc gcaagagcca cggtttccct cgtgctgagc acgacttctt
    17401 cggcatgttt cagcgtcaga aatgcattcc tgatttccag cctgatggcg cggcgaagat
    17461 tctcctgctc tgcaaccgca atatcccgct gaagctcagc ggtcctgaat tccgcttttt
    17521 tcaggccgcc gtccatgatg ttccacgagg cggaaagccc ggcattccag ccccattccc
    17581 agtcgtcagc tgaaggatct tcgtccccgg gattggtccc cttgtaattc gcgaaggcac
    17641 ggatctccgg gcggtaatcc gagcgtgcaa cacgtatgtt ctcctcccgc atttggacgt
    17701 tcagctgggc ctgcctcaat tccggacggt tacgtgcagc cgtctcaatc aggccttcat
    17761 atgtgaaatc caccggacga tattccagct ccccctcaat gacaaaatca tcaccatcgg
    17821 caaggatgag atccttgaga gcggccatga ccagctcgta ccggttccgg gcctgcacca
    17881 gccggggcct ttcattggca agattaacct gtgcggtcag ccggtcatat tcagaggcgg
    17941 cggcattctg ataccgggtt cgcgcctgct gctcgaaatc agacagctgc tgcacagaag
    18001 cctcggcaac ttcaacggct ttctcggcat agagcgcctc atagaaggcc tgacggatgt
    18061 cgcgtatcag ttcagcctgc tggcgccggg tgtcctctga tgcgaaggcc cggtaatcgc
    18121 gcgcggctcg caaagcggcg cgaacgcctc cgccggaata gagcagctga ctggcctcca
    18181 ctccggccgc atagttgtct tccctcccga agggctcttc catgccgggg aaagtcggaa
    18241 cttcatcgag ccgtgtgaaa gaactggagg catcaagcga gggcagcgcc cgcgcgcgaa
    18301 cctgccggac cttttcgacg gccagcagct ctcctcggcg cgcatttgaa agatccagtg
    18361 aacgctgcag cccgagggct atgcagtcct caagcgaata ggcatgttct gcagacggcc
    18421 ggtcggcggc gaccagcagc accggaaaca gaaaacagaa taatatattc ctccacatag
    18481 ggatcacacc catatccttg aaacaaaaac cggaattcta tccggccgaa ggacaaaata
    18541 aagaacaacc tctacaaatt tccccctgct tcccactgcc gaagatgaga ctggataaaa
    18601 gcgatgatct gctggttttc atcccggccg cccggactct ttatggaaat aggcggaccg
    18661 aatgcaaaat gaataggttt gctgcggtcg atgggcccga aagccttggc ccaccgcgca
    18721 tttccccacc agtcggtctt aagcgccaca ggaatcacgc gcacgcccgc ccgggatgcc
    18781 agcttcacgc caatggagtt aaactcctct ggaataaatt ccaccttgcg ggtgtgctgc
    18841 gggaaaatga tgatggaaac accgcgagca agcttctcgc acccttcgtt catcacccgc
    18901 ttgaaatcct cccggggatt ggtgcgcccg acgggaatgg gatcccgggc gcgcataatc
    18961 ggcccgaaaa acgggcccgt caccagccgc tccttcacga catacgtcgc ctccatgacc
    19021 gacgcaacga aaaacggcat caccatggtt tcaagtgtgc tcatatggtt gccgataaag
    19081 acaatcggct ccttcagatc gcggaaatgc tcgagtccgg tgatatgaaa acggccgccg
    19141 cactcctcga tgagctgata aaactcatac ga
//
LOCUS       DAOQWR010000037        19169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3239_length_19169_cov_5.618133, whole genome shotgun sequence.
ACCESSION   DAOQWR010000037 DAOQWR010000000
VERSION     DAOQWR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3239_length_19169_cov_5.618133"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2539)
                     /locus_tag="PKK36_04610"
     CDS             complement(<1..2539)
                     /locus_tag="PKK36_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR93875.1"
                     /translation="MVQTKLMVFCYMFLLGIVFAKLVPADATWLNAIHILTGVFFLAL
                     AAHYVNRRREIRQGYKYELAWSRVAGWVLVVLAALSLGYGRYIKADTVPDTRMATVVL
                     RGSAADLKVEAQLTDTSRVRIRKTSAVAEDVKLRLLGVLEARLAVLSDDGVPQMDSEG
                     RWRFTRVKQEYASDVIVIGKDDPVGTDYLVPQPFDRITGIEVVEGSSSGHLGIYRISN
                     HLASFVRLGMNSMPVTVLGKISQDPWVYDFKTVLHISPLFIQYRPGGPFYRVEGGTIR
                     ANLDTRVDNYRRFARSEGYGLDVVVTAPLFEARPQANPGDFDQRQYLQNNNIYGQMGL
                     RQARGGPPPIVLVAPEGGEPARGHPLVRFSLKIRDDMLRVFKQTLPYPHSAFVGAVTL
                     GLRYGLQGVECIFSPAHGDPLLGQRCEQTIADEFKHSGVNHVLAVSGLHVTIITILFT
                     GIFTLLRMSRRAFVPLIVMILVIFAIITGARPSTLRAVIMNSLFLLTWAYLNKGLVAS
                     ALLGVPVAAFLILYHNPLMIVDPSFTLSFGAILSLVLITPPVYKLLSMLRGNNFLVAI
                     LILLGISAALGWSWFLVTSLRFWIPFLIVCTLLCLGARAASRKGKKLFGNLAYVQIPV
                     AIGTLVASQAAIQWGMMAPLSAAYFSRWAAGGSYANLLALPLIGIVVQLGAMAGLLGM
                     IPVIGPFIGLLLNAANWVFSTGFLWLAHFSVKLFPFPFVRKISPVHLLLYYLILAVFV
                     WHEPLWSVFKSQFGRKKGHRHWAYAMVALLIVLLGAAFATRPHYRHGNDLNLTMLSVN
                     YGNALLVETPGDKKFLIDAGMVEHERGMFNSAERTILPYFSYRSIMNL"
     gene            complement(2526..2753)
                     /locus_tag="PKK36_04615"
     CDS             complement(2526..2753)
                     /locus_tag="PKK36_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93876.1"
                     /translation="MRGGPGAIAYMLVISLLMGLLVVLIHPDYRDTARSMLRGRTEQS
                     SIWIDNERYYPEVVLAPSGEDREEGADNGSN"
     gene            complement(2750..3076)
                     /locus_tag="PKK36_04620"
     CDS             complement(2750..3076)
                     /locus_tag="PKK36_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93877.1"
                     /translation="MTAEPDKQPPDGPGGARSVLDEYPVIGRILLLVNAVGCLAYLSW
                     LVMANKRIFYTQEGVIYLLPFLLFFFVFIYIFGKPAEKEEDQPDEEQAAAQDGAAEQA
                     EERIKQ"
     gene            complement(3120..3875)
                     /locus_tag="PKK36_04625"
     CDS             complement(3120..3875)
                     /locus_tag="PKK36_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNR93878.1"
                     /translation="MAQVLTREKTLQAWGSSAAREGRVLRVISIVEWPLALALTAVGA
                     INLWKDQTWGFLVAGGILLLLAIGHRAQLKENFRDAGSVRAGQAGESRVSRMLAKMLP
                     GNMYIINDLTVKFGGTSAQIDHLVVGPKGLFVIETKNWSGTVRGDERRDMWEQIPSHG
                     RKKIIKVGNPVAQNERHVNVLRSWLRARGSAWNDLYSVVTILSPHTRLEIDNQTTPLL
                     PVKATARFIERFQAPALHSAREVREMVDLLLRK"
     gene            complement(3890..5095)
                     /locus_tag="PKK36_04630"
     CDS             complement(3890..5095)
                     /locus_tag="PKK36_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNR93879.1"
                     /translation="MNTENKTTESGSNKFLQYRTAIIIGVVTLAAIGAVLWLALTGGD
                     KEVEVYEDAARPAGDINVAQVIDAIQQKDAVARAGYRYKVVIEDESKTGTAGIARIGG
                     LVIFVEGTPAVGDVLIVEVTQMRATTANAVIVEKVGTMEVPKRAAGRGRSRETSIDGK
                     VYSGIIEDVGSKGDGIIHIDGKVVFVEGSTKGQDVTFIITYEGDRFSSGQLVTAEEAE
                     QLGGEIVKIEPGRGRSPDRRDSGSRRAPPADSPVKVGQAYRGVVDDMGSKGDGIVHVQ
                     GKVVFIPGTSKGDEIVFIVEDDGPRVATGRKISAEEAEGLQVEDVAVSAAPKKSSKAA
                     PKSSGGSVVVGETYSGTVEDVGSKGDGIVRIDGKVVFIPGAEKGQSVTFKVTEDKGRT
                     AIGALVPAE"
     gene            5213..6181
                     /gene="amrS"
                     /locus_tag="PKK36_04635"
     CDS             5213..6181
                     /gene="amrS"
                     /locus_tag="PKK36_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNR93880.1"
                     /translation="MRVQCTLCPKGCVIGPGESGDCRIRVNLDGLLTAVTWGFPCSIH
                     VDPVEKKPLFHFLPGTAILSLATVGCNLHCKNCQNWEISQAQPEHTRVSPLEAADLPA
                     LAQSCRCPSVAYTYTDPVAYYEYALDCSRRAREAGLKNVLVTAGYINDGPWRELCRHV
                     DAANIDLKAFSDRFYRDICDATLQPVLNSLVTAKSLGLAVEVTNLVIPTLNDSDRDLR
                     ELVRWIKTNMGSDTPLHFSRFFPQHLMRHLPPTPPETLERARSIARAEGLDYVYVGNV
                     TTEGGSDTFCPACGKLLIARRRYHILENSLQHGHCPACGKEIAGVW"
     gene            6175..6354
                     /locus_tag="PKK36_04640"
     CDS             6175..6354
                     /locus_tag="PKK36_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNR93881.1"
                     /translation="MVTARRDFLKMMALAAAAACSGVSFFIQKVFRAARPREWTGRTK
                     SLPRRVIERPGRWAG"
     gene            6371..7984
                     /gene="amrB"
                     /locus_tag="PKK36_04645"
     CDS             6371..7984
                     /gene="amrB"
                     /locus_tag="PKK36_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNR93882.1"
                     /translation="MKKMTHILKSILAAGCLCAASSCDKPATALPIPETEQTPAQSPA
                     AAREKTILRSRLIGRWYPRASYDLSVEIDGFLKNVPDTPREPIMALIMPHAGYRYSGQ
                     IAAHAARQIQDKAYRRVIVIGPSHSQPMKNFCSVPDVTHYETPLGQVPLDVELIEKLR
                     ARGDFFQCIPEAHVWEHSVQVQVPFLQHMLKDFTLVPILTGHLDEKTTRAIADILKQE
                     MDNETLLVISSDFTHYGASYGFIPFTTDIPANIEKLDMSVFDPIRRKDLSGLYRQLDV
                     SRATVCGRCPLGILLAMLPRDAKIHLLKYDTSGRITGDWSASVSYLAAAVTGLWAPDR
                     PPAPKQNTVVRPAPAAKPTLNPEDKEKLLRLARISIEFWLTNRSKPSPNEVWNELTPG
                     MQQTLGAFVTLKKNGALRGCIGEIIPHREVYKAVIEHAINAAAFDRRFAPLRKEELDA
                     VEIEISALLPPRAVDSYRDIEIGRHGIVLKKGGRQAVFLPQVAAEQGWTLEETLSQLA
                     MKAGLPPDGWKQNAEFMVFEAIVFHEGDE"
     gene            7977..10859
                     /locus_tag="PKK36_04650"
     CDS             7977..10859
                     /locus_tag="PKK36_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNR93883.1"
                     /translation="MNKTGFRQALIPFALGFFSIAAQALLFRWFFTAFESNELGISAF
                     FFSWLLWVTVGALAARLPVLRGMTGPNFARSLWAYIPAFLLQRLLILNARPICGVAVY
                     EAFPFARLFAAAILLNAPVSFVTGLLFTLSCRWLGPSSDQRVARVYSAEAIGSVLGGI
                     WVTGLLALLPDTESAALLTMLALAVLYGLQRFDSRMPRFLILAAALALSAGLPQAGRW
                     WGFRTARARWAALLPGSVCDGRFTTPQNTYLHGIQNGQFNVVCGSTIIESVPDDERAG
                     ELTAIHLAQKPDAKNILVVGEGSYALARRLLLIPQVEQVTWLPTDPAYGRKLPEALPD
                     KLKANGGRFSIPGQDIQQYLSRAGPLFDLVILHLPDPVALDLHRFFTQQFLRKIRARL
                     SPNGAVSLRTAGGENFLGSERVFLGASLLATMESVFPHVVFKPGDETWFMASAADGLS
                     QSAQELADRFARIDGAGAVYPPEGLLSIFSENRVAFQLGKYREAMQDQPPGSLIATTD
                     SPRALFHTLQLLTREISLDARITPRLLRLSRHGWLLFIFAIALCVLLRSARGPAGLTE
                     YDGRYLVFTTGLAGLALNIMLMILYQSRFGSIYLHIGLITAAFMLGIWIGSIGGRIAL
                     RHRPGAAAILSAGLVIAEAGFCLLIPAAGRVIGPVMFMLLFLVAGGLSGAFIPAAAHM
                     LGNAGMDEYRMGAVVEMTDHLGGAAGGVLTGLILLPVAGIRRTFLILAALLLSNLVPL
                     IPSPSRRTAPRGPGWIFAALWLAGVLLFGAYLVREPAPQESAAAPSSILEMAGRRGLS
                     RHQLPDGSIYFTARDKDKEYFLFTAESKGYGGPMTLSVMINTNGILNDFRVVRHSETP
                     AYMERLAPWMKSLAGKNIFSERLPAEVDTVTGATATSAAIRNALAAAGQRFLERRAGR
                     PAVEPQPGTAPAARTARKADLPKIRSMIHEGELSGREALHYIRLDD"
     gene            10804..11940
                     /locus_tag="PKK36_04655"
     CDS             10804..11940
                     /locus_tag="PKK36_04655"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNR93884.1"
                     /translation="MRENFPAAKPCTIFASMTKHIIIIMLLSACAQADVIRLKNGREI
                     EGIIQKETVSEVTIKVGPGLVTLKRSAVASLEKGGAGELETKWRAEHFLHPKYIPAGL
                     EDTARDFQNLQTRHGEALAARAALDRIPKRHRELSDELARTEAAFRETRAQMNATDPK
                     RHNKLYNDLVTRHNTLNNRVLDIQDQMETLAPARLEHAKTIGAFLSALTQFKLKMHKT
                     QKSDYPEGSPEQIFFAQLRRAILPLEKDVEFSRIPYDEENRHAIITAMINGHTEGRFL
                     LDTGATFVSLSSTMADRLGLDLSGPHETTVTLADGSRVTAKPVMLDSMQVGDARVEQV
                     PAMVLPNSPAPGMDGLLGMSFLQEFSIHFDAANQRLILQRFRPE"
     gene            12079..14673
                     /locus_tag="PKK36_04660"
     CDS             12079..14673
                     /locus_tag="PKK36_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR93885.1"
                     /translation="MRGWIKGGFWGAVVITLLWSLNPRAAVEKTLEPGTVEIQFMGNA
                     GPVQGALEDAIREFERLSRERHAIDPDYPIYRVVSGQNASRNQTEDPTRFLVSLAGGM
                     PPDVIFFDRFAISEWAARGAFTPLDDFVARDLEAWNAWTSAGSKSLPPWPGALEEPPR
                     AKGAARLAAVEPVHQTNYYPACWDEAVYRNPLTGEAHLYGVPNNADNRILLYNKDILI
                     RHGYTNENGEARPPRTWEELEEMALAMTERDEKGNIKTIGFIPNYGNVWLYMYGWQAG
                     GKFMSEDGKRCTLDDPRIEYALDFMTRLYDLLGGAKEVYAFQTTFQGAELDPFILGKV
                     AMKIDGVWIMPALAFYGKDINLGAAPAPMPKAELDAGRAPISWVGGFAYAIPATAKEK
                     KGAWELIRYLSSQRAHEILMYSESLTSLSQGRPYLPRQFPHIRQNEWTYDTFILNNDA
                     VEPQFKQAMRVYNDLLANSYYRPVTPVGQKLWNAQIWAMEDAVFHKKTPKEALGYYAK
                     IVQRDLDAILQPPEGTPVRSWNWFFWLYGILIVLAIAAVYWWDTHVAIRKKNSVMLEG
                     KKGNYFRAQWKEGVICALPWILGFIIFTGGPLLFSIVISFCRFDVLNDAVFTGLQNYI
                     FMLKGDELFWKSLWNTLFMVIGIPLGMAAGLGIALLLNLKIRGVAVWRTLFYLPSIVP
                     AVAASILWIWIFNPQSGLLNMALDPVLDLFGAKSPQWLQDEHTSKWALIIMGLWGAGG
                     GMIIWLAGLKGISQSYYEAADIDGASTWQKFRFITIPMLTPYIFFNLIMGMIGTFQIF
                     TQAFIMTAGGPVNSTLFYAYHLFNNAFRYLHMGYAAAMAWFLFLIVLILTGIQMKLSG
                     RWVHYEGA"
     gene            14694..16328
                     /locus_tag="PKK36_04665"
     CDS             14694..16328
                     /locus_tag="PKK36_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR93886.1"
                     /translation="MMHQSRRAARLLTVYGLLIGGSAIFILPFLWMAGTSVKVDREMF
                     SENLRLFPMRPVPAQVSPYLDDKYFPPAPESKMVEEAVTANMPELPEHLQVEQDAAGI
                     LSDGIWQKLRQVIPERTWQNDPREAIQKEVTPELVAAQLEKVYRHFSLSAVRVRSHDL
                     QEIEVTAGKPVSEFWNVTGGANLADGMEGKTPYADLVYNFKNNRNGMIQLSRTIDLPF
                     AADRLFRTFISLRADDSWHRLEFFIEKNGRLLRGVRPEFLGNFQWSVITLQEKIEGED
                     DTSKIRLWIAMREIDRGPQYESRPDKMKLTVQMHRSSQAGAWWAKCQRNYRGALDYIP
                     FWRYTATSVFLVVLNIIGNIFACSLVAYAFARLQWPGRGFCFGLMLATMMIPPQITMI
                     PYFLIIKHLGWYNTLTPLWFVSFFGNAFNIFLLRQFMMGIPRDLEDSARIDGCNFLQI
                     YWYVIMPLIKPTLACIAIFTFMGVWNDFMGPLIYLSDQRLYPLSLGLYALNVQAGGNF
                     GMMMAGALLMTLPVVAIFFFAQKYFIQGVTLTGMKG"
     gene            16331..17473
                     /locus_tag="PKK36_04670"
     CDS             16331..17473
                     /locus_tag="PKK36_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93887.1"
                     /translation="MKLSFALMIAIVAGTVMAEDSGYFLSMSQKGQLIWDGRMEDSDG
                     ICYDVSIVPGYAPPVRYGWRNLKQAGGNLHDYVEAKKYKRIKRQSGDALEWAFDDCLS
                     DFTVKGSGKAWKKYFRKANDRTKKRVFGWWLSYPWALMQSTVDNVVRIPCGLAGTVIG
                     TGWGLVVVPGYHMLDSAVAATWNGGVEGVILPVSGLAWNTVISPPMALVGQKPSPERV
                     DGFWISMVDTIPAQSSEINKDSIAAIAEFGMKLHAELAPMDQERAEQEKLERLESQRL
                     HQQFASNRTAFAESRKARVNEIINDESLRPALDQIVHGRWNYDRFRQHRKEIIKRMMD
                     DGLSEQEAGRAMRDIEAAPLNHYPIRRPPAKTDPVTESIEVIKDIE"
     gene            complement(17510..18031)
                     /locus_tag="PKK36_04675"
     CDS             complement(17510..18031)
                     /locus_tag="PKK36_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93888.1"
                     /translation="MMGTEFYWTVIVALCAGGWAMMIAVRDRKAQAIKHSNSIIDRLL
                     ESDKLIVENPDIQMYLSGTAQEDEAHFRRPDVLKDMQFFKAKAYLYGQLNLFDEILSA
                     AAQTKSSRFLLGPPEIIEIADWEMFIRHKLTHPMCRSILRNESAIFGRALQDFWKKHE
                     SDILAQAPDSYSW"
     gene            complement(18128..19114)
                     /locus_tag="PKK36_04680"
     CDS             complement(18128..19114)
                     /locus_tag="PKK36_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93889.1"
                     /translation="MLLPAALLLTSSGCATYDACHARFAEPDYVVTRRFFTEVPDKVV
                     ILPFAARRDRERDTRNAQVCRTTFYQHFAVQSFEDVDLCRVDKLLNSQQNESETLVDD
                     FLGIVRGLDVVGITSLLELQPFFGKRHRRYHEIFRSLVETVAGQELQADAYVLGISRS
                     YGRVYAVLASSIALSTRLEMRSVTDDGLLWRADGRHRNMAAVLTINPLAIPNLLVDVW
                     KNSRGRALTLLAYQVYGDVVATMPDLDGPVEPYVETSRKKTPWFRKPSLFRLLREGHL
                     PPGRRLPFIMEKRGWYQCEGPDGKPVWIFRSDARLTDADGRPLAGARPGNAD"
BASE COUNT         4078 a         5923 c         5392 g         3776 t
ORIGIN      
        1 ccagattcat aatgctccgg taggagaaat aaggcagaat cgtgcgttcc gccgaattga
       61 acatgccgcg ttcatgctcg accatgcccg catcaatcag gaacttcttg tcgccgggcg
      121 tttcgaccag cagcgcattg ccataattga ccgaaagcat cgtcagattc aggtcattcc
      181 catgacggta atgaggacgg gtcgcgaagg ccgcgcccag cagcacgatc agaagcgcaa
      241 ccatggcata ggcccagtga cggtggccct ttttgcggcc gaactgggat ttaaacacag
      301 accagagcgg ttcatgccag acaaacaccg caaggatcag ataatagagc agcaggtgca
      361 caggcgatat tttccgcacg aacgggaagg gaaacaactt caccgaaaaa tgcgccagcc
      421 agagaaatcc cgttgaaaac acccagttgg ccgcgttcag aagcaggccg atgaagggtc
      481 cgatcacggg gatcatgccc agcaggcccg ccatggcgcc aagctgcacc acaataccaa
      541 tgagaggcag cgccagcagg ttggcataag agccgccggc cgcccatcgg ctgaaatagg
      601 ccgccgaaag cggcgccatc atgccccatt gaatcgcagc ctgagatgcc accagtgtgc
      661 ctatcgccac cggaatctga acataagcaa gattgccgaa cagttttttc ccttttcggc
      721 tggccgcgcg cgcacccagg cacagcagcg tgcagacaat caggaacggg atccagaacc
      781 ggagggaggt caccaggaac cagctccagc ccagggccgc cgagataccc agaaggatca
      841 ggatcgccac gaggaagttg ttccctcgca gcatcgacag cagcttgtag accggcgggg
      901 tgatcagcac aagggacaga atcgccccga acgagagcgt gaacgagggg tccacgatca
      961 tcagcggatt gtgataaaga ataagaaatg ccgccaccgg aacacccagc agcgcggagg
     1021 ccaccagccc cttgttgagg taggcccagg tcagcaggaa aagactgttc atgatcactg
     1081 cgcgcagcgt ggaggggcgg gcgcccgtga tgatcgcaaa tatcaccagg atcatgacaa
     1141 tcagcggaac gaacgcgcgc cgcgacatgc gcagcagcgt gaagatgccg gtgaaaagga
     1201 ttgtgatgat ggtcacatgc agtcccgaaa cggccagcac gtgattcacg cccgaatgct
     1261 tgaactcgtc cgctatcgtc tgctcgcacc gctgccccag cagcggatcg ccgtgggccg
     1321 ggctgaaaat gcactcgacc ccctgcaggc cataccgcag accaagggtt acggcgccca
     1381 caaaggcgga atggggataa ggaagcgtct gcttgaacac ccgcagcatg tcgtcgcgaa
     1441 tcttcagaga gaaacgcacc agcggatgac cgcgggcggg ctcgccgccc tcgggggcca
     1501 ccagcacgat gggcggaggc ccgccccgcg cctgccgaag ccccatctgt ccgtaaatgt
     1561 tgttgttctg aagatactgc ctctggtcaa agtcgccggg attcgcctgc ggacgggcct
     1621 caaacagcgg ggctgttacc accacatcaa gtccgtagcc ttcgctgcgg gcaaaacgcc
     1681 ggtagttgtc cacgcgcgta tccagattgg cgcggatggt gccgccctca actctgtaga
     1741 aaggtccgcc gggacggtac tggatgaaca gcggagaaat atgcagcaca gtcttaaagt
     1801 cataaaccca cggatcctgg gatatctttc ccagcacggt gaccggcatg gaattcatgc
     1861 ccagacggac aaaggaggcc agatggtttg atatccggta aatgccgaga tgccccgacg
     1921 aagagccctc caccacctcg atgcccgtaa tccggtcaaa cggctgcggc accagataat
     1981 ccgtgccgac gggatcatcc ttccctatga cgatgacatc gctcgcatat tcctgcttca
     2041 cccgggtgaa gcgccagcgg ccctcggaat ccatttgcgg caccccgtca tccgaaagca
     2101 ccgccaggcg cgcctccagc acgcccagga gccgcagctt cacgtcttca gccacggcgg
     2161 atgtcttgcg gatgcgcacg cgcgacgtgt cggtcagctg cgcctccacc ttcaaatccg
     2221 cggctgatcc ccggaggacc accgtggcca tgcgcgtgtc cggaaccgtg tcggccttga
     2281 tatagcgccc gtatcccagc gacagggccg ccagcacgac gagaacccat ccggccaccc
     2341 tggaccatgc cagttcgtac ttgtagccct gcctgatttc gcgacggcgg ttcacataat
     2401 gggccgcgag agccagaaaa aatacgcccg tgagaatatg aatggcattc agccatgtcg
     2461 catcggccgg aacaagcttg gcaaagacaa tgccaagcag aaacatatag cagaaaacca
     2521 tgagcttagt ttgaaccatt gtccgctcct tcttcacggt cttcccccga cggggccagc
     2581 acgacctcgg gatagtaccg ctcgttgtca atccagatcg aagactgctc ggtgcggccg
     2641 cgcagcatgc tgcgcgccgt gtcgcgataa tccgggtgaa tcagaaccac cagcagaccc
     2701 atcaaaagcg aaataaccag catataggca attgccccag gaccacctct cattgcttta
     2761 tcctttcttc ggcttgttcg gctgcgccat cttgggcggc cgcctgttct tcatccggct
     2821 gatcctcttc cttttcggcc ggcttcccaa atatgtaaat aaaaacaaaa aagaaaagca
     2881 ggaacggcag cagataaatc acgccttcct gcgtgtaaaa tattctcttg ttcgccatca
     2941 ccagccacga cagataggcg aggcagccga cggcattaac cagcagcagt atccggccga
     3001 tcaccggata ctcgtccagc acgctgcgcg cccccccggg gccgtccgga ggctgcttgt
     3061 ccggttctgc cgtcatattt ccgcccctgc tttccagtta aaaggcacag agtaaaccat
     3121 catttccgca atagcaagtc caccatctcc cgcacctcgc gggcgctgtg cagcgccggc
     3181 gcctgaaatc gctcgatgaa ccgcgccgtc gccttgaccg gcagcagggg cgtcgtctgg
     3241 ttgtcaatct ccagacgggt atggggagag aggatcgtga ccacggagta aagatcgttc
     3301 cacgccgaac cccgggccct cagccagctg cgaagcacat tcacatgccg ctcgttctgg
     3361 gcgacaggat ttccaacctt gatgattttc ttccggccgt gcgagggaat ctgctcccac
     3421 atgtcccggc gctcatcccc tcgcaccgtt ccgctccagt tcttggtctc gatgacaaag
     3481 agtcctttgg gccccaccac cagatgatca atctgggctg atgttcctcc gaacttcacc
     3541 gtaagatcat tgatgatata catgttcccg gggagcatct ttgccagcat tctggacacg
     3601 cgcgattcgc cggcctgacc ggcacggacc gatcccgcat cgcggaaatt ctctttcagc
     3661 tgtgcgcggt ggccaatggc cagaagaagg agaatgcctc ccgccaccag gaaaccccag
     3721 gtttgatcct tccagagatt gatcgcgccg acggccgtca gcgccagggc cagcggccat
     3781 tcaacaatgc tgatcacgcg caggacccgg ccctcacgcg cggcggacga accccaggcc
     3841 tgaagggttt tctctctggt caggacttgc gccacagccg ttaacgacgt tactcggccg
     3901 gaaccagcgc tccgatggct gtgcggccct tgtcctcggt caccttgaac gtcacgctct
     3961 ggcctttttc ggccccggga ataaagacaa ccttgccgtc aatgcgcaca atcccgtcgc
     4021 ccttggatcc cacgtcttca acggtgccgg agtaggtctc gcccaccaca accgaaccgc
     4081 cggaagactt tggcgcggcc ttcgacgatt tcttgggagc agcggacacg gccacatcct
     4141 ccacctgcag tccttctgcc tcttccgcgc tgatcttgcg gccggtggcc acgcgggggc
     4201 cgtcatcttc aacaatgaaa acaatttcgt cgcccttgct cgtgccgggg atgaaaacaa
     4261 ccttgccctg aacgtgcaca attccgtcgc ccttgctgcc catgtcatcc accacacccc
     4321 tgtaggcctg gccgactttc accggcgaat ccgctggcgg cgcgcgacgg gatccggaat
     4381 ctctgcggtc cggggaacgg cccctgccgg gttcaatctt cacgatttca ccgccaagct
     4441 gttcggcttc ttccgccgtg acaagctgac cggaggagaa acggtcgcct tcgtatgtga
     4501 taatgaatgt gacatcctgc cccttggtgc ttccctcgac aaaaacaacc ttgccgtcaa
     4561 tgtgaatgat gccgtcaccc ttggagccaa catcctcaat gattccggaa tagactttcc
     4621 cgtcaattga tgtctcgcgg ctccggcctc ggccggcggc gcgtttgggg acttccattg
     4681 ttccaacctt ctcgacaatc acggcattgg cggtggtggc ccgcatttgc gtgacttcca
     4741 cgatcagcac atcacccacg gccggagtgc cttccacgaa gatcaccagg cccccgatgc
     4801 gcgcaatccc cgccgtgccg gtcttggatt catcttcaat caccactttg tagcggtatc
     4861 ccgccctggc gaccgcatcc ttctgctgga tggcgtcgat cacctgcgcg acattgatat
     4921 cgccggccgg ccgggccgca tcctcataga cttcaacttc cttgtctccg cccgtaagcg
     4981 ccagccagag cacggcgcca atggcggcca gagtcaccac gccaatgatg atcgcggtgc
     5041 ggtattgaag gaatttgtta gaacccgatt cggtggtttt gttttctgta ttcatagtgt
     5101 gcaaactcta gcagttaatc ccccttcttc aacataattc tgctttcggc acgggaaatg
     5161 aaaagggcac aaattcgctg acacatcccc gactgaaggc tatagttccc acatgcgggt
     5221 tcagtgcaca ctgtgtccaa aaggttgcgt catcggtccg ggcgaaagcg gcgactgccg
     5281 cattcgcgtc aatctggacg gcctcctcac agcggtgacc tggggcttcc catgctccat
     5341 ccatgtggat cccgtcgaaa agaagcccct tttccatttc ctgccgggga ccgccatcct
     5401 ttcgctggcg accgtcggat gcaatctgca ctgcaaaaac tgtcagaact gggagatctc
     5461 gcaggcgcag ccggagcata cccgggtctc tccgctggag gcggcggatt tgcctgcgct
     5521 ggcgcaatcc tgccggtgcc cttcggtggc ctatacctat acagatccgg ttgcctacta
     5581 cgaatatgcg ctggactgct cccggcgagc ccgcgaggcc ggcctgaaaa atgtgctggt
     5641 gaccgccggc tatatcaacg acggaccctg gcgcgaactc tgccgccacg tggatgccgc
     5701 caatattgac ctgaaagcgt tctcggaccg gttctaccgc gacatctgcg atgcgacgct
     5761 gcagcctgta ctgaattcgc tggtcaccgc aaaatccctt ggtcttgcgg tcgaagtaac
     5821 caatctggtg atccccaccc tcaatgacag cgaccgggat ctgcgggagc ttgtccgctg
     5881 gatcaaaacg aacatgggtt cagacacgcc tcttcatttc tcgcgtttct tcccgcagca
     5941 cctgatgcgg catctcccgc cgactccacc cgagacgctg gaacgcgccc gcagtatcgc
     6001 cagggcggag ggccttgatt atgtttatgt cggaaatgtg acgacggaag ggggcagcga
     6061 cacattctgt ccagcctgcg gcaaactgct gattgcccgg cgaaggtatc acattcttga
     6121 aaacagcctg cagcacggac actgccccgc ctgcggaaag gaaatagccg gcgtatggtg
     6181 actgcacgga gagatttcct gaaaatgatg gccctggccg cagcggcagc ctgcagcggg
     6241 gtcagctttt tcatacagaa ggtgttccgc gccgcgagac cccgcgaatg gaccggccgg
     6301 acaaagtccc tgccccgcag ggtgatcgaa cggccggggc ggtgggccgg ataatggagg
     6361 aagagaagcc atgaaaaaaa tgacacacat tctgaaaagc attctggccg ccggctgtct
     6421 gtgcgcggca tcgtcatgcg acaaaccggc cacagcgctt ccgatcccag agacagaaca
     6481 gaccccggcc cagtcccccg ccgcggcgcg cgaaaaaacc atcctccgct cccggctgat
     6541 cggccgctgg tatccgcgag caagctatga tctcagtgtt gaaattgacg ggttcctcaa
     6601 aaacgtgccc gacacgccgc gcgaaccgat catggcgctc attatgcccc atgcaggcta
     6661 tcgctattcc ggccagatcg ccgcccatgc cgcgaggcag attcaggaca aagcctaccg
     6721 gcgggtcatt gtgatcggac cgagccacag tcagccgatg aagaacttct gcagcgtgcc
     6781 ggatgtgaca cattacgaaa ccccgctggg tcaggtgccc ctggatgtgg aattgattga
     6841 aaaactgcgc gcgaggggtg actttttcca atgcatcccg gaagcccatg tgtgggaaca
     6901 cagcgtgcag gtccaggtcc ccttcctgca gcacatgctg aaggatttca ctctcgtgcc
     6961 cattctgacg ggccatctgg atgaaaaaac cacgcgcgcc attgccgata tcctgaaaca
     7021 ggaaatggac aacgaaacac tccttgtcat cagctcggac ttcactcatt acggcgccag
     7081 ctacggcttc attccattca cgaccgatat ccccgccaat atcgagaagc tcgatatgtc
     7141 cgtttttgat cccatccgca gaaaagacct gtcaggcctc taccggcagc tggatgtgag
     7201 ccgggcgacg gtctgcggac gctgcccgct gggaattctt ctcgccatgc ttccccgaga
     7261 cgcaaagatt catctgctga aatacgacac gtcgggccgc atcacgggag actggtccgc
     7321 atctgtcagc tatcttgcgg cggcagtcac cgggctctgg gcgccggacc gaccgccggc
     7381 cccgaaacag aacaccgttg tccgccccgc gccggcggcg aagccgacgc tcaatccgga
     7441 ggacaaggaa aaactgctga gactggcgcg aatctccatt gagttctggc tcaccaaccg
     7501 atcaaaaccc tcgcccaacg aggtatggaa cgaactcaca cccggcatgc agcagaccct
     7561 cggggcgttt gtgactctga agaaaaacgg cgcgctgcgc ggatgcatcg gagagatcat
     7621 tcctcaccgc gaagtataca aggccgtcat cgaacacgcc atcaatgccg ccgccttcga
     7681 cagacgcttc gcacctctcc gtaaagagga gctggacgcg gtcgaaattg aaatttccgc
     7741 cctgcttccc ccgcgcgcgg tggattcata ccgggatata gaaatcggcc ggcacggcat
     7801 cgttctgaag aaaggcggac gacaggccgt ttttcttccg caggtggccg cggaacaggg
     7861 ctggacgctg gaagaaacac tgtcccagct tgccatgaaa gcaggtcttc cgccggacgg
     7921 atggaaacag aacgccgagt tcatggtctt tgaagccatt gtatttcatg agggcgatga
     7981 ataaaaccgg tttccgacag gccctgatcc cattcgcgct ggggtttttc tccatcgcgg
     8041 cgcaggccct gctgttccgc tggtttttca ccgcctttga aagcaatgaa ctggggatca
     8101 gcgctttttt cttctcatgg cttctgtggg tgaccgtcgg cgcgctcgcg gcgcgactgc
     8161 cggttctgcg cgggatgacg gggccgaatt ttgcacggag tctgtgggcc tatattccgg
     8221 cttttctcct tcaacggctg ctcattctga acgcgcggcc gatctgcggt gttgccgtct
     8281 atgaggcttt ccctttcgcc cgcctgtttg cggccgccat tctgctcaat gcgcccgtca
     8341 gcttcgtcac cggacttctt ttcacgcttt cctgccgctg gctggggccc tccagcgacc
     8401 agcgtgtcgc tcgcgtctac agcgccgaag ccatagggag cgttctgggc ggaatatggg
     8461 tcaccggcct gctcgccctc ctgccggata cggaaagtgc ggcattgctc accatgctgg
     8521 cgcttgcggt cctctatggg cttcaacgct tcgattcacg aatgcctcgc ttcctgatcc
     8581 tggccgcggc gctggcactt tcggcggggc ttccgcaagc gggccgatgg tggggattca
     8641 ggacggcccg ggcgcgctgg gcggcgctcc tgcccggttc tgtgtgcgac ggtcgcttca
     8701 ccactccgca aaacacctat ctgcatggaa ttcaaaacgg gcagttcaat gtcgtctgcg
     8761 gaagcacgat catcgaaagc gtccccgatg acgagcgcgc cggggaactc acggccatcc
     8821 atctcgcgca gaaaccggat gcaaagaata ttctcgtggt cggcgaagga tcctatgccc
     8881 tcgcccggcg cctgctcctg atcccgcagg ttgagcaggt gacatggctc ccgaccgatc
     8941 cagcctacgg acggaaactc cccgaggcgc tgccggacaa gttgaaggcg aacggcgggc
     9001 gcttcagcat tcccggccag gacattcagc agtatctgtc ccgcgccggc ccgctgtttg
     9061 atcttgtgat tcttcatctt cccgacccgg tcgcgcttga cctccaccgc tttttcacac
     9121 agcagtttct ccgcaaaatc cgcgcccgcc tgtctccaaa cggcgcggtc agcctgcgaa
     9181 cggcaggcgg cgagaacttt ctgggaagtg agcgtgtttt tctgggagcc tctctgctgg
     9241 ccacgatgga atcggtattc cctcatgtcg tcttcaagcc gggcgacgaa acgtggttca
     9301 tggcatccgc ggcggatggg ctatcccaga gcgcacagga actggccgac cggtttgcac
     9361 ggatagacgg cgcaggagcc gtttatccgc ctgaagggct gctttccatc ttctccgaaa
     9421 accgcgtcgc ctttcagctc ggaaaatatc gcgaggccat gcaggaccag ccccccgggt
     9481 cgcttatcgc cacaaccgac agcccgcgcg cgctgtttca cacccttcag ctgctcaccc
     9541 gcgaaatcag tctggatgcc cggataacgc cccgactgct ccgcctgtca cgacacggct
     9601 ggctgctttt catctttgcc atcgccctct gcgtgctgct gcggagcgcg cgcgggccgg
     9661 cgggcctcac ggaatacgac ggacgctatc tcgtcttcac aaccggtctt gcaggactgg
     9721 ctttgaatat catgctgatg attctctacc aaagccgttt cggctccatc tatctgcaca
     9781 tcggcctgat tacggccgcg ttcatgcttg ggatttggat cggaagcatc ggcggaagaa
     9841 tcgcgctgcg ccaccgcccc ggggccgccg ctatcctctc ggccggactc gtcattgcag
     9901 aggcgggctt ctgcctgctg atccctgcag cggggcgtgt gatcggtccc gtaatgttca
     9961 tgcttctttt cctggttgcg ggcggcctga gcggggcctt tataccggcc gcggcacaca
    10021 tgctcggaaa tgccgggatg gatgaataca gaatgggcgc ggtggtggag atgacggacc
    10081 acctcggcgg cgcggccgga ggggtgctga cgggactcat tctgcttcct gtggcgggca
    10141 tccgccgaac attcctcatt ctggccgccc tcctgctcag taatctggtt ccgctgatcc
    10201 cgtccccttc acggcgcacc gccccccgcg ggccgggatg gatcttcgcc gccctctggc
    10261 tggccggggt gctcctgttt ggcgcatacc ttgtgcgcga acccgcccct caagaaagcg
    10321 cggcggcccc atcctcgatt ctggaaatgg ccggcagacg cggcctgagc aggcatcagc
    10381 tcccggacgg ctcaatctat ttcacagccc gggacaagga caaagaatac ttcctcttca
    10441 cagccgaaag caagggttac ggcggtccca tgacgctctc cgtcatgatt aacaccaacg
    10501 gcattctcaa cgactttcgc gttgtgcggc acagcgaaac ccccgcctac atggaacgcc
    10561 ttgcgccatg gatgaagtcg ctggccggaa agaatatttt cagcgaaaga ttgcccgctg
    10621 aagtagacac cgtcaccgga gcaacggcca cgtcggcagc catccgaaat gcccttgcgg
    10681 cggccggtca gaggtttctc gagcggcggg cggggcggcc ggccgtggag ccccagccgg
    10741 gcaccgcgcc cgcggcccgc acagcccgca aggcagacct tccgaaaatc agaagcatga
    10801 ttcatgaggg agaactttcc ggccgcgaag ccctgcacta tattcgcctc gatgactaaa
    10861 cacatcatca tcatcatgct gctgtcggcc tgcgcgcagg ccgacgtgat ccgcctgaag
    10921 aacggacgcg aaatcgaagg catcatccag aaagaaaccg tatccgaagt caccatcaag
    10981 gtcggaccgg ggctcgtgac cctcaagcgg tcagccgtcg catcgctgga aaaaggcggc
    11041 gcaggagaac tggaaaccaa atggagggcc gagcattttc tgcatccgaa atatattcca
    11101 gccgggcttg aagacaccgc ccgcgacttt cagaacctgc agacccggca cggcgaggcc
    11161 ctcgccgcgc gcgcggcgct cgaccgcatc cccaaacggc accgggaact gagtgacgaa
    11221 ctggcccgca cggaagccgc tttccgcgag acccgcgcgc aaatgaacgc caccgacccg
    11281 aaaagacata acaagcttta taatgacctt gtgacccggc acaacacgct caacaatcgc
    11341 gttctggata ttcaggacca gatggaaacg ctggcccccg cacggctgga acacgccaaa
    11401 accatcggag cttttctgag cgcccttacg cagttcaaac tgaaaatgca caaaacccag
    11461 aagtcggatt atccggaggg cagtcccgag cagatattct tcgcgcagct ccggcgcgcc
    11521 atccttccgc ttgaaaaaga cgtggaattc agccgcattc cctatgacga agaaaaccgt
    11581 cacgccatta tcaccgccat gatcaacgga catactgaag gacgattcct gctggatacc
    11641 ggagccacct tcgtttcgct ctccagcacc atggccgaca ggctgggact ggacctgtcg
    11701 ggtccgcatg aaaccacggt caccctggcc gacgggagcc gggtgacggc aaaacccgtc
    11761 atgctggatt caatgcaggt cggcgacgcc cgtgttgaac aggtccccgc catggtgctt
    11821 ccgaactcgc ccgcgcccgg aatggacgga ctgctgggaa tgagcttcct gcaggagttc
    11881 agcatccatt ttgacgcagc caatcaacga ctgatcctgc agcgcttccg gccggaataa
    11941 aaacttccaa tcattggaag tttttccgga aaaagttcca accattggaa gtttttgaac
    12001 acaaagttcc aatgattgga agtttgcgtt tggggcgcgg gtcgggcaga atgaaacaat
    12061 aatttcagga tgcccgtgat gagaggatgg atcaaaggcg gtttctgggg agcagtggtc
    12121 ataacgctgc tgtggtctct caatccccgc gcggcggtcg agaaaacgct ggagccgggc
    12181 acggttgaaa tccagttcat gggcaacgcc ggacccgtgc agggcgcgct cgaagacgcc
    12241 atccgcgagt tcgagcggct cagccgcgag cgccatgcta ttgatccgga ctatcccatc
    12301 tatcgcgtgg tctccggaca gaacgcctcg cgcaaccaga ccgaagaccc cacccgcttt
    12361 ctcgtcagcc ttgccggcgg catgccgccc gatgtgattt ttttcgaccg ctttgcgatc
    12421 agcgaatggg ccgcgcgcgg ggcgttcacg ccgcttgatg attttgttgc gcgcgatctg
    12481 gaggcatgga atgcctggac ctctgcaggt tcaaaaagtc ttccgccgtg gcccggcgcg
    12541 ctggaggagc cgccgcgcgc gaaaggcgca gcccgcctcg ccgccgtcga gcccgtgcac
    12601 cagacaaatt actatcccgc ctgctgggac gaggcggtgt accgcaatcc gctgacgggc
    12661 gaagcccatc tgtacggcgt gccgaacaac gcggacaacc gcatcctgct ttacaacaag
    12721 gacatcctga tccgccacgg ctataccaac gaaaacggcg aggcccgccc gccgcgcacg
    12781 tgggaggagc tcgaggaaat ggcactggcc atgaccgagc gcgatgaaaa aggaaacatc
    12841 aagaccatcg gattcattcc aaactacggc aacgtctggc tctacatgta cggctggcag
    12901 gccggcggta aattcatgag cgaggacggc aagcgctgca cacttgacga tccgcgcatc
    12961 gagtacgcgc tggatttcat gacacggctt tacgatttgc tcggcggcgc aaaggaggtc
    13021 tacgctttcc agaccacgtt ccagggcgcg gagctcgatc cgttcattct cgggaaagtc
    13081 gcgatgaaaa ttgacggggt gtggatcatg ccggccctcg ccttctacgg caaagacatc
    13141 aatctgggcg ccgcgccggc gccgatgccg aaggccgaac tcgatgccgg gcgcgccccg
    13201 atctcgtggg tcggcggctt cgcctatgcc attcccgcaa ccgcaaagga gaagaaaggc
    13261 gcatgggagc tgatccgcta tctttccagc cagcgcgcgc acgaaatcct gatgtattcg
    13321 gagagcctca cttcgctgag ccagggccgg ccgtacctcc cgcggcagtt cccgcacatc
    13381 cggcagaatg aatggaccta tgacaccttc attctcaaca acgatgcggt cgaaccgcag
    13441 ttcaaacagg ccatgcgcgt ctataatgat ctgctcgcaa attcgtatta ccgtcccgtg
    13501 acgcccgtcg gacagaagct gtggaacgcg cagatctggg cgatggaaga cgcggtcttt
    13561 cacaagaaaa cgccgaagga agcgctgggt tattatgcga aaattgtgca gcgcgatctc
    13621 gacgccatac tgcagccgcc cgagggcacc cccgtccgga gctggaactg gttcttctgg
    13681 ctgtacggaa tcctgattgt gctggcgatt gctgccgtct actggtggga cacgcatgtc
    13741 gccattcgga agaagaacag cgtcatgctg gaaggcaaaa agggaaacta cttccgtgcg
    13801 cagtggaaag aaggtgtgat ctgcgcgctg ccttggattc tgggattcat tattttcacc
    13861 ggcggcccgc tgctgttttc gatcgtgatc agtttctgcc ggtttgatgt tctgaacgat
    13921 gcggttttca ccggacttca gaattacatt ttcatgctga agggcgatga gctgttctgg
    13981 aagtcgctct ggaacacgct gttcatggtg atcggcattc cgctgggcat ggccgccggg
    14041 ctgggcattg ccctgctgct gaaccttaaa atccgcggtg tggccgtgtg gcgcacgctg
    14101 ttctatctgc cgagcatcgt ccctgccgtg gccgccagca ttctgtggat atggattttc
    14161 aatccgcaga gcggactgct caacatggcg ctggatcctg tgctcgacct gtttggcgca
    14221 aaaagccccc agtggttgca ggacgagcac accagcaagt gggcgctgat catcatgggc
    14281 ctgtggggtg cgggcggagg catgattatc tggctcgcgg gactgaaggg aatcagccag
    14341 agctactatg aggcggccga cattgacggc gccagcacct ggcagaaatt ccgcttcatc
    14401 acgattccga tgcttacacc ctacatcttc ttcaacctga tcatgggcat gatcggcacg
    14461 ttccagattt tcacgcaggc cttcatcatg accgcaggcg ggccggtcaa ctccacgctg
    14521 ttttacgcct atcacctttt taacaacgct ttccgttatc tgcacatggg ctatgcggcc
    14581 gccatggcat ggtttctgtt cctgattgtt ctgatcctga caggcatcca gatgaaactt
    14641 tcgggccgct gggtccatta cgagggcgca tgaacgaagc cggaaaatca gctatgatgc
    14701 accagtcgcg ccgcgccgcg cgcctgctga cggtgtacgg cctgctaatc ggcggctccg
    14761 ccattttcat cctgcccttc ctgtggatgg ccggcaccag cgtaaaagtg gaccgcgaga
    14821 tgttcagtga gaacctccgt ctgttcccca tgcgaccggt cccggcgcag gtctcgccgt
    14881 atctcgacga taaatatttt ccgcctgcgc cggaaagcaa aatggtcgaa gaagccgtga
    14941 ccgcaaacat gccggaactc ccggaacacc ttcaagttga gcaggatgcc gccggaattc
    15001 tatcggacgg aatctggcag aaactgcgtc aggttatccc tgaacgcaca tggcaaaacg
    15061 atccgcgcga agccatccaa aaggaagtga ccccggaact cgtggccgcg cagcttgaaa
    15121 aagtctaccg ccatttcagc ctgagcgcgg tgcgcgtccg cagccacgat ttgcaggaaa
    15181 ttgaagtcac cgcaggaaaa ccggtttccg agttctggaa tgtgactgga ggcgcaaacc
    15241 ttgctgacgg aatggagggc aaaacgccct atgccgatct tgtttacaat ttcaagaaca
    15301 accggaacgg aatgattcaa ttgtcccgca cgattgatct gccgtttgca gccgaccgcc
    15361 ttttccggac attcatttca cttcgtgccg acgactcctg gcaccggctc gaattcttca
    15421 tcgagaaaaa cggccgactg cttcggggtg tccgcccgga atttctgggc aatttccagt
    15481 ggagcgttat cacgcttcag gaaaagattg agggcgagga tgacacgagc aaaatccgcc
    15541 tgtggattgc tatgcgcgaa attgaccgcg gcccgcagta cgaaagccgg ccggacaaaa
    15601 tgaagctgac cgtgcaaatg caccgcagct cgcaggcggg cgcgtggtgg gccaagtgcc
    15661 agcgcaacta ccgcggggcg cttgattata ttcccttctg gcgctacacg gccaccagtg
    15721 tttttctcgt ggttctgaac atcatcggca acatcttcgc ctgctcgctg gtcgcctatg
    15781 cctttgcgcg gctgcaatgg cccggacgcg gattctgctt cggcctgatg ctggccacga
    15841 tgatgattcc gccgcagatc accatgatcc cctactttct catcatcaag catctcggct
    15901 ggtacaacac gctgacaccg ctctggtttg tcagtttctt cggcaacgct ttcaacattt
    15961 tcctgctgcg ccagttcatg atgggcattc cgcgcgacct ggaggattca gcccggattg
    16021 acggatgcaa ctttctgcag atttactggt atgtcatcat gccgctcatc aagcccacgc
    16081 tcgcctgcat cgccatcttc accttcatgg gcgtgtggaa cgatttcatg gggccgctga
    16141 tctatctcag cgaccagcgg ctctatccgc tttcgctggg cctctacgca ctaaatgtgc
    16201 aggcgggcgg gaacttcggc atgatgatgg caggggccct gctgatgacc ctgcccgtcg
    16261 tcgccatttt ctttttcgcc cagaaatatt tcattcaggg cgtaaccctt acgggaatga
    16321 aaggatgacc atgaagctga gttttgcgtt gatgatcgct attgtcgcgg gaactgtgat
    16381 ggccgaagac tctggatatt ttctttcgat gtcccagaag ggccagctca tctgggacgg
    16441 acgcatggaa gacagcgacg gcatctgcta cgacgtttcc atcgtccccg gctatgcccc
    16501 gcccgtgcgc tacggctggc ggaacttgaa acaggccggc ggcaacctgc acgactatgt
    16561 cgaagcaaag aaatataaac gcatcaaacg ccagagcggc gatgccctgg aatgggcctt
    16621 tgatgactgc ctttccgatt tcaccgtgaa gggatcaggc aaagcgtgga aaaaatattt
    16681 tcgcaaggcg aatgaccgca cgaaaaagcg cgtcttcggc tggtggctct cctatccgtg
    16741 ggcgctgatg cagagcaccg tggacaatgt ggtcagaatt ccttgcggtc ttgccggaac
    16801 ggtcatcggc accggctggg gactggtcgt ggtgcccggt taccacatgc tggactccgc
    16861 cgtggccgcc acctggaacg gcggcgtgga gggtgtgatt ctgcccgtgt ccgggctggc
    16921 gtggaacacg gtcatttcgc cgccgatggc cctcgtcgga caaaaaccct cccctgagcg
    16981 cgtagacggg ttctggatca gcatggtgga cacaattccc gctcaatcct cggaaatcaa
    17041 caaagacagc attgccgcca tcgccgagtt tggaatgaaa ctgcatgcag agctcgctcc
    17101 catggatcag gaacgggcgg aacaggagaa actggaacgg cttgaaagcc agcgcctgca
    17161 ccagcagttc gcatccaacc ggacggcgtt tgctgaaagc cgaaaagcca gagtgaacga
    17221 aatcataaat gatgaaagcc tccgtccggc tctcgatcag attgttcatg gtcgctggaa
    17281 ctacgaccgc ttccgccagc accgcaagga aatcatcaaa cgaatgatgg atgacgggct
    17341 gtcagagcag gaagcgggaa gggccatgcg cgacattgaa gcggccccgc tcaatcatta
    17401 ccccatccgc agaccgccgg cgaaaaccga ccccgtcacc gaatccatcg aggtcataaa
    17461 agatattgaa tagcgcgcgg ggaaaggatt ttcggcccga cctgctctgt taccaggaat
    17521 agctgtccgg ggcctgagcc aaaatatccg attcgtgttt tttccaaaag tcctgcagag
    17581 cccgtccgaa gattgcgctc tcgtttctca gaatggagcg gcacatcgga tgggtgagtt
    17641 tatggcgaat aaacatctcc caatcggcga tttcaataat ctccggcggg cccagcagga
    17701 agcggcttga ttttgtctgg gcggcggccg acaggatttc atcaaacagg ttcagctgcc
    17761 catacagata ggccttggct ttgaaaaact gcatatcttt caggacatcc gggcggcgga
    17821 agtgggcctc atcctcctgt gcggttccgg aaagatacat ctgaatgtcc ggattttcga
    17881 cgattagctt atcgctttcc agcagacggt caatgatcga attggaatgc ttgatcgcct
    17941 gcgccttccg gtcgcggact gcgatcatca tagcccatcc acccgcgcac aatgcaacaa
    18001 tcacggtcca gtagaattca gttcccatca cacccgacct ccatgctttt tcgctccgct
    18061 cccgcctgtt tctgccagaa actagcagaa ctcccggcca aaaccagcta aaagaaatcc
    18121 gatgaattca gtccgcgttt cccggccggg ccccggccag cgggcggccg tcggcatccg
    18181 tgaggcgcgc atcggaacga aatatccaaa caggctttcc gtcgggcccc tcgcactggt
    18241 accatccgcg cttttccatg atgaacggca ggcggcggcc cggcgggaga tgtccctcgc
    18301 gcagcaggcg gaagagcgag ggcttcctga accacggcgt tttcttccgg ctggtctcca
    18361 cgtacggctc gacgggaccg tccagatcgg gcatggtcgc caccacatcg ccatacacct
    18421 gataggccag cagggtcagc gcccggccac gtgaattttt ccagacgtcc accagcaggt
    18481 tcggaatcgc cagtggattg atcgtcagca cggccgccat attccggtgc cttccgtccg
    18541 cgcgccacag cagcccgtcg tcagtgacag accgcatttc cagccgcgtg gaaagcgcga
    18601 tggatgaggc cagcacggca tagacccggc cgtagcttcg gctgatgccc agcacatagg
    18661 catcggcctg cagctcctgt cccgccaccg tctcaaccag agagcggaat atttcgtgat
    18721 agcggcggtg gcgcttaccg aagaacggct gcagttcgag caggctggtg atgcccacca
    18781 catccagccc tcgaacgatg cccagaaaat catcaaccag agtttccgac tcgttctgct
    18841 gagagttcag aagtttgtcc acgcgacaga gatcaacatc ttcaaatgac tgtacggcga
    18901 aatgctgata aaacgtggtc cggcatacct gtgcgttccg ggtgtcgcgc tcgcgatcac
    18961 gccgggcggc aaacgggaga atgacaacct tgtcgggcac ctcggtgaaa aaccgccggg
    19021 tcaccacata atcgggctcg gcaaagcgcg catggcaggc gtcatacgtt gcgcagccgg
    19081 aggatgtcag cagaagggcc gcgggaagca gcaacttcca atgcctggaa cttttttgcg
    19141 aaaatcttcc aatgattgga actttttgc
//
LOCUS       DAOQWR010000038        18980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3277_length_18980_cov_5.078890, whole genome shotgun sequence.
ACCESSION   DAOQWR010000038 DAOQWR010000000
VERSION     DAOQWR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18980
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_18980_cov_5.078890"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /gene="nuoF"
                     /locus_tag="PKK36_04685"
     CDS             <1..1394
                     /gene="nuoF"
                     /locus_tag="PKK36_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNR93890.1"
                     /translation="AYVMHDGYSALRKAVQDDSAQIVKTVTDSGLRGRGGAGFLTGMK
                     WKFCREAAGDQKYIVCNADEGDPGAFMDRSVLEGDPHAVIEGMAIAGFAVGASRGVVY
                     CRAEYPLAVKRLQKAIDQSREKGFLGKSVCGSPFEFDISIMQGAGAFVCGEETALLAS
                     IEGKRGTPRIKPPFPAQQGLWGKPTVINNVETFANVPHIINHGAEWFASIGTEKSKGT
                     KVFALAGKVKNTGLVEVPMGTTIRELVFGPGGGMLHKKLGLKGVQLGGPSGGCIPAAM
                     ADTPIDYESLQSTGAIMGSGGVIIMDEKTCMVDVARYFTGFTVSESCGQCTPCRVGLK
                     RMHEVLTRVVEGEGVPADLEFLGNMGNTICSTSLCGLGQTAPNPVLTTLRYFRDEFDA
                     HVHRRECPAHVCTTLMHYEVIEDMCIKCGACAKVCPAGAVTWERGKTARIDPAKCTRC
                     NACNTACKYMAIR"
     gene            1394..3493
                     /locus_tag="PKK36_04690"
     CDS             1394..3493
                     /locus_tag="PKK36_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR93891.1"
                     /translation="MKITLNDKVIETRPGKTVLEAALDAGEYIPNLCYHPDVRPVGAC
                     RLCIVQIKGMRGFPPACTTKAADGMEIRTDSPELIRFRKNLAWLAVSEAPEAMKANGQ
                     FRKVVEYIGLEDVLPGYIPQSKGRPPDESDPLYIRDPDLCILCGRCVRMCSDVRQVGA
                     IGIVNRGIESEVGTSFAEPVLQSTCKFCGACAEVCPTGAIRDRIPMCEIDREAKIVPC
                     REACPAHVDVPRYVYLISQGRFDEALEVIRERLPFPFVLGAVCTHLCEAACKQCQVSG
                     QPVAIRELKRYVASRDSGSWRRKLKVVAPTGKKIAVIGSGPAGLSCAWFLRRAGHEVT
                     VYEALPRPGGMLRASIPRYRLTEDDLDREINVIREAGVEIKTGVRVESPDELLKNGAD
                     AVFIAIGAEKGTRMTFEGSPEDPRIRDGLTVLEDINDGRLTKMKGIIAVVGGGNVALD
                     VVRSARRLGAERVILLYRRSRSEMPASKEEVDFAEEEGVEFHFLVNPVKVFGKPDKLE
                     VECIRMELGEPDASGRRRPVAVEGSEFRIAADQLIAAIGQQTEVPASFGIETDKWGCV
                     KVDKDTYATSRKGVFAAGDAVTGPASVIEAIDAARKAASSIDLYLGGDGNVEKPIAPP
                     EKMDPRLGSEKGFSGRKRRPVPVIPVEERTSTFNEVEKTYSEEDAVNEASRCLRCKLR
                     LEIGRAPMPPENKVKIR"
     gene            3490..4032
                     /locus_tag="PKK36_04695"
     CDS             3490..4032
                     /locus_tag="PKK36_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93892.1"
                     /translation="MNSCAWTSTILGTLLGVFYLWLVLKPASWRNAAAKFPRSVWAGR
                     ILTAVAVLWVSWLLFHMNLGRLSFLKSAVWYLAPICYLLIIFFMDDLLAPRALGGLML
                     LAANPIFYHVRWHDSNLRLIIVVLGYIIVIKGMLLVLSPFQFRRLVNRFLNTDSQCRR
                     WGGVGLAFSAFLLILGLFVF"
     gene            complement(4326..4985)
                     /locus_tag="PKK36_04700"
     CDS             complement(4326..4985)
                     /locus_tag="PKK36_04700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR93893.1"
                     /translation="MLAKDQILFSDNHLLAVSKPGGLLTQPSGTTEDSVEAQAREWVK
                     REFNKPGAVFLEAVHRIDREVSGVVLFARTSKALSRLNEQIRDRKTRKIYHALIEQAL
                     PKKAGTLTHWLIHGDHRALVGCETDPDAKQAVLHYRVLGTAWKMWLIEIQLETGRYHQ
                     IRAQLAAEGCPIAGDAKYGAKSRFAENTIALHHRQLEIVHPVKHTVIVITAPYPAAWP
                     S"
     gene            complement(4987..5844)
                     /locus_tag="PKK36_04705"
     CDS             complement(4987..5844)
                     /locus_tag="PKK36_04705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR93894.1"
                     /translation="MSSNDYELLDSGNGCKLERFGKIILERPCSQAVWAPQKPHLWKT
                     ATASFSRTGGLNWKGREALPASWTVSVSGIRMKLSSTDFGHLGIFPETRALWDWIAET
                     VRQAAGEKKRQIQFLNLFAYSGGATLAAAKAGAACCHLDASKGMVEWARENASLNNLQ
                     DAPVRWIVDDVNKFLAREARRERRYDAILLDPPSFGHGHRGELYKIEKDLLITLENVR
                     SVLSPDPVFVLLTSHTPGFSPIVLRNLLMQVLGPGTVSCGEMLLTGARDVFEVPSGNW
                     ARWLPGNCG"
     gene            complement(5938..7026)
                     /locus_tag="PKK36_04710"
     CDS             complement(5938..7026)
                     /locus_tag="PKK36_04710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR93895.1"
                     /translation="MNRKYYVVLIMALSAGLLSVGCSKKEEAPAVEKSLAADANLFAP
                     AAMPSTDPNAVAVTVNGTEIKQGEVDTEVGKLMMRAQGRVPPERMMQMRGQMVDDVTE
                     ALVVKMLLEKAIDEEKITATEEEIAEGRAKVAGMLEPGVTLEQQLEEVKMSEEDFRKS
                     LVLDIRINKLLKAKAGEVEISDEKVKEFYDENSQRFEMPESVTASHILIMTDAQDDED
                     AKAAKKAKIEELRQQILGGADFAELAKEHSDCPSKARGGNLGTFGRGQMVPPFEEAAF
                     SQELGKVGDVVETQFGYHLILVEKREPARTLPLEEVSERIREGLQMQSQQAAVKDYIE
                     QLKTEADIVYPEGSGPVELPVLEAPAGE"
     gene            complement(7125..8102)
                     /locus_tag="PKK36_04715"
     CDS             complement(7125..8102)
                     /locus_tag="PKK36_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR93896.1"
                     /translation="MKNSLSVFFYEAFAEEEHSLRACLPPEISADFTWKSIQEAGHAV
                     PPAALISIRTQSVVPAAWTRSLHGILSRSAGYDHVADWLKKNPCGIKAGYLPEYCVRA
                     VAEQAALLWMSLLRKLPQQQRQFPVFLRDGITGRECAGKTLLVTGVGRIGYEIARIGQ
                     ALNMRVYGVDIVEKHSDISYMQIDEGLRQADVVVCSMNLTAENHGYFSYERLSQCRPG
                     VVFVNIARGEMSPPVDLLRLLDEGIAGGVALDVYPEEPRISLLLRSGQPSDDPLVRVL
                     REIGNRPNAVLTPHNAFNTEESVEQKSRQSVQQTVHFLNTGSFLWPVPG"
     gene            8273..9622
                     /locus_tag="PKK36_04720"
     CDS             8273..9622
                     /locus_tag="PKK36_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR93897.1"
                     /translation="MAENEVADLQVKEEAAVTRGARPNTDCNFVVQLLVGAIGTGIVV
                     AAVLPLKGKGPLASYMHGIVMERGPVQYFELFMFFMTAAFLFLKSRIVRAQFRVLSQG
                     PVNPTIDLTDDDQIQDLRNEIVNHEAFSWSILLNRIERIIGLWLGSKDISRVSTWTST
                     ESSNDQNTAESTYSLVNVLVWAIPIMGFIGTVQGLGAAVSGFSAFLAGAAELSAIKDA
                     IGNVTIGLGVAFDTTLLALILVTFLMFPLASVQRKEQTLFGEIDNYIDDTMLSRFPSQ
                     EQQPIVIENLEDSIEAAFRRYIPDPDRYDEVFTRSIDKAANMVEGRFSGLAKNYEGTL
                     NALADRLQSSVAGAGDALQGAIAGVVANIDKSSQKSTEAAEQLSTRIAEVGKLASSVQ
                     EMLQLEASIEKSLKGISASDEFQQTLKEIREHLAATDQFCKQLNKPRVITFKEEIAG"
     gene            9657..11702
                     /locus_tag="PKK36_04725"
     CDS             9657..11702
                     /locus_tag="PKK36_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93898.1"
                     /translation="MGIRNNSSGCSADALARVLLLVLLCCAGLFPAYAYEGSLPAKVA
                     VSGLQGPAGMTVHPETGDIYVAEKTAGRISVIRSGAASPVISTGWSLDPNIPFWAVSR
                     ELSKEALVKPALNNPGSVAIATNGTLYVCESTPSGRLLEFAPDEQGNYTKGKIIPIPW
                     IDRPFSWTDVKVARDGRLFLVGGVAEADVLHFGTVLMRDEQGDWWVVDYGPFINFSSV
                     YLSRNEDIVIVCERDLGGVVWWDAVRHLPIGTLEESGKNISADAVSLMVDGAFVVGQN
                     LAAGGARAVRVDPLTGEQKDLISGLDRIGGLLLSSAKNSLYVSDEAKGVIVEASVSPP
                     SEGMEYLLQRSLDGYQMGEGFTPRQAPGFLKNFFSQVGSAQTGATGEEEGLDAVTDRL
                     TMSFSLREFAAKIPLIAGKIETRSLEEASASVSEDPLTLIEFVIFFPGKTIASGDLAT
                     PSLAYFAAQRKSGKVERTRELFSGLNIRQRKSGGEWMAGGDNARIMVPVSSVGLEKSA
                     TGMDLNLAFLGLGIYSDYYLRLTSGQENTGEIVVEGLDGTREVYRAVFTEMAKVGMGA
                     EVGSEIKNLVVAGFDPMQQDSGMGWLNIGRSPVNVAVATSMSELHEFAGMDSKITAMM
                     KKKDREIISTRPEDVVEGASPQPVAPVEAGDSPETQPAVSNEADAAAVPSSAEEAAP"
     gene            11723..12310
                     /locus_tag="PKK36_04730"
     CDS             11723..12310
                     /locus_tag="PKK36_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93899.1"
                     /translation="MAVKMDLSSFMCILIMAIGCLVVILVVNVIIIVSNPENVQITSL
                     FRGAYEDYDSEEGPGATIGQPKYGNLFKDPFYVDVHSDQIIVYPEKNVVTLRQLEAGN
                     NAFEDLLNKIEPMKDKTYVIMVLRPKSARMSRLVRRAIRHRGIDVGQELFEAGRKIPE
                     IKSTAEINAEFSGITNAPAEDAGAEMEAGAEAAAQ"
     gene            12383..12976
                     /locus_tag="PKK36_04735"
     CDS             12383..12976
                     /locus_tag="PKK36_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93900.1"
                     /translation="MTCLVGVLVLIIILTSIDASQTKLLIPTPMAHETTKRPVFIECR
                     GDMLYPIDLPEIRRKMDEELSRIEKESLGNKEKMIQMMSQAMIETDYYKVDLSYALAG
                     QNLLIPKPEAAGLTLDNITASTMSMTALPGWLDTLVKQLNPEEDIVTFLVRDDSFRVF
                     KKARGIAWLLNVEVSYELLDMNDPIKLGLGGEVSLAQ"
     gene            12984..13283
                     /locus_tag="PKK36_04740"
     CDS             12984..13283
                     /locus_tag="PKK36_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNR93901.1"
                     /translation="MPETYSECIQCGDCCRWSGFVYLNEDDIRLAARFLNMEESAFIQ
                     KYAVLGPNRHQLCLIPGAGRDCVFLCGNNCRIYPARPQQCRDFPLRWGTCPHRQA"
     gene            13361..13789
                     /gene="fabZ"
                     /locus_tag="PKK36_04745"
     CDS             13361..13789
                     /gene="fabZ"
                     /locus_tag="PKK36_04745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNR93902.1"
                     /translation="MAVMELEDILEILPHRYPMLLVDRVIECDFEKRIVGIKNLTFNE
                     IFFQGHFPGHPVMPGVLQIEAMAQVGGILINKIAAAEKAVAYFMAIDNAKFRQMIRPG
                     DQMRMELEIVKMRKTVCVMHGKAFVDGKLAVEADMTFFRS"
     gene            13797..14570
                     /gene="lpxA"
                     /locus_tag="PKK36_04750"
     CDS             13797..14570
                     /gene="lpxA"
                     /locus_tag="PKK36_04750"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006790128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNR93903.1"
                     /translation="MTSIHQTAIVDPGAELGERVSIGPYCVVGADVRIGDDAELMSHV
                     VVGGHTTIGCQCRIFPFASIGQQTQDLKFDGRISYVEIGSRTTIREYVTVNLATSEGD
                     TTRIGSGCLLMAYSHVAHDCQVGDSVIIANCGTLAGHVIVEDQVIVGGLSGIHQFVRI
                     GRLSIIGGCSKVTQDVPPFMMADGHPVSIHGLNSVGLKRRGVSAAARKQLKDAFKLIY
                     RQDLLTTQAVEQIQSWPEKTPELEHLIAFVKSSDRGIVK"
     gene            14567..16504
                     /gene="gspD"
                     /locus_tag="PKK36_04755"
     CDS             14567..16504
                     /gene="gspD"
                     /locus_tag="PKK36_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNR93904.1"
                     /translation="MIWRRRLPLMLLFLLVPCVLLPSLLPAEQNGGRSQVNFNFDRVD
                     IRLLVKLVGEITGMQFVVDDSVKAEVTVVTPAQIPADEVYPLFVSILESSGYSIIRKG
                     TVSHIVPLASREVPSAPVRTGDTSDAVGIITKVIRLQYIDAVEMRAVLLPMVSGGKTG
                     SLSAFGPTNHLLITDTAENIARIEEIIAELDKKGSNRIAEVVPLKNASAAEVAAQINM
                     AIEGGESAGRKVSRHMQQVADGRGAMPTEMTVVPVTHANSLLLVGTPANIEEIKRMLE
                     QIDTESLAARGPLSVIFLKYLSAEAAAKSLNALLEKSAEKDQKRSIAIEPDIANNALL
                     LHGSPRDCDWVRELVDQLDQVPQQVLVEILIAEVSVGKNLDLGVEWSTIDVAAEGRTT
                     VLGRSRPGETDTLMDYITQGVLPQGLAVGVARGTYTDASGAVQPSIPVLLRALAQNRD
                     IKILSNIPLWAQNNTEASVSVVENIPILKSTIEGGAGTARDTIQNIDRIDVGIKLKLT
                     PHVNPDREVTIELNPSIEAIIDTAASAELPFTPTITKREVATTVTVKDKTTIAISGLI
                     RDDQITTVYKVPLLGDIPLLGMLFRSTSRRAERTNLLIFVTPHIVTEAESALAARRSW
                     EDRSEAGSLATNYMSTIELRE"
     gene            16506..18194
                     /locus_tag="PKK36_04760"
     CDS             16506..18194
                     /locus_tag="PKK36_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR93905.1"
                     /translation="MRPLGQILIERGIISSSQLEEALARQQALPGRRIGEILIEAGWV
                     SPADVLKALGVQYQCEVLDIIPPEMLDSELVSSLPVEWARSHEMLPVRHREQPAVLTA
                     DPTRINEQDDLSLLTGCPLIPVLALPGLIREAIENCYFMRRETASEVMAGLPAPEAVQ
                     PSAAAGATDLLAVATDAPVTRLVNLILLEAVRRRASDIHIEPLEDSLRVRYRIDGMLY
                     EQSAPPRHFEAALVSRLKVMGHLDIAEKRLPQDGMARVSVGERDIDIRISTIPVAEGE
                     RIVLRLLNRESTLLPLGRLGMPDHVLRPFGMLLKLNHGAIWVTGPTGSGKTTTLYAAL
                     QELDSEHLNILTIEDPVEYQLAHISQISVKPKIGLTFAQGLRHILRQDPDIILVGETR
                     DLETAEIAVRASLTGHLVFSTLHTNDAIGAIVRLVDMGIPRYLVSAATSGVLGQRLIR
                     TLCPECRKPMELSAEDAAGLGPCAQRLKGQPVYDAGNCSACMSGYSGRTGIYELLMMD
                     DAIRDAIRHEADLMALNRAVAGRGFSPLTEDAVEKIQQGITSPAEVLRVLGRFP"
     gene            18202..>18980
                     /locus_tag="PKK36_04765"
     CDS             18202..>18980
                     /locus_tag="PKK36_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR93906.1"
                     /translation="MKTYEYRGYQTSGHAIRGLIEASGLKDARERLAEGGILVERISS
                     ASVRRRTLPFRRSRGLDSETRSSVYRELSSMMRAGLTLTQSLNLLIEAMEEAPATRVL
                     LAGMRDRVQEGASLAQALAEVSGGTHEMELAAVRAGERAGNLDVVLERLAAFLEEQRE
                     IRERVQTALIYPSIVLGMAVLIALLMFGVMIPRFASLFEESQIPLPPVTRYILSAGQF
                     LKYLLFALIACGAASTGFIALKSSDPQFRILLDRFWFNLPL"
BASE COUNT         3925 a         5228 c         5702 g         4125 t
ORIGIN      
        1 aggcctatgt catgcatgac ggctattcag ctcttcgcaa agccgttcag gatgattctg
       61 cgcagatcgt gaaaaccgtc acggattccg gcctgcgcgg gcgcggcggc gcgggattcc
      121 tcaccggcat gaaatggaaa ttctgccgag aggctgcggg cgatcagaag tatattgtct
      181 gcaacgcgga cgagggcgat cctggcgcat tcatggatcg aagtgtgctg gagggcgatc
      241 ctcacgccgt gatcgagggc atggccattg ccggctttgc ggtgggcgcg tcgcgcggag
      301 tggtctactg ccgcgctgag tatccgctgg ccgtcaagcg cctgcagaag gcgattgacc
      361 agtcccgtga gaagggattt ctcgggaaat cggtctgcgg cagtccgttt gaatttgaca
      421 tttccatcat gcagggcgca ggggcgtttg tgtgcggcga ggaaacagcg ctgctggcct
      481 ccatcgaagg caagcgcggc acgccgcgca tcaagccgcc gttcccggcg cagcagggcc
      541 tgtgggggaa acctaccgta atcaataatg tcgagacgtt tgcaaacgtg ccgcatatca
      601 tcaaccatgg cgcggaatgg tttgcatcca tcgggacaga gaaaagcaag gggacaaagg
      661 tttttgcgct ggccggcaag gtgaaaaaca cgggccttgt ggaagttccg atgggcacca
      721 cgatccgcga gctggtgttc ggaccgggcg ggggaatgct tcacaagaaa cttggactga
      781 aaggcgttca gctcggcggg ccttccggcg gatgcattcc cgccgcgatg gccgacacgc
      841 ccattgacta cgaatccctg cagagcaccg gcgcgatcat gggctccggc ggcgtgatca
      901 tcatggacga gaaaacctgc atggtggatg tggcgcgcta tttcaccggg ttcacggttt
      961 cggaatcctg cggccagtgc acaccctgcc gcgtggggct taagcgcatg catgaggttc
     1021 tgacccgtgt ggtcgaaggc gagggtgtgc cggccgatct ggagtttctg ggaaacatgg
     1081 gcaatacgat ctgctcgact tccctttgcg gcctgggcca gaccgcgccc aatccggtgc
     1141 tgaccacgct tcgctatttc cgcgatgagt tcgatgcgca tgtgcaccgc agggaatgcc
     1201 cggcgcatgt ctgcacaact ttgatgcact acgaagtcat tgaagacatg tgcatcaaat
     1261 gcggagcctg cgctaaggtt tgtcccgcgg gcgcggtgac ctgggagagg ggaaagactg
     1321 cccggattga tccggcaaaa tgcacacggt gcaatgcctg caacacagcc tgcaaataca
     1381 tggcgattcg ctgatgaaaa ttacgctcaa cgacaaagtg attgagaccc ggcccggcaa
     1441 aacggtgctg gaggccgcgc tggatgcggg ggaatacatt cccaatctct gctatcatcc
     1501 ggatgtccgc ccggtgggcg cctgccggct ctgcattgtg cagatcaagg gcatgcgagg
     1561 tttccctccc gcctgcacaa cgaaggcggc cgacggcatg gagatacgga cggattcccc
     1621 tgaactgatc cggttccgga agaatctcgc ctggctcgcc gtcagcgagg ccccggaagc
     1681 catgaaggcc aatggccagt tcagaaaggt tgttgaatac attggccttg aggatgtgct
     1741 ccccgggtac atcccgcagt cgaaaggccg tccgccggat gaatccgatc cgctgtacat
     1801 ccgcgatccg gatctctgca tcctctgcgg ccgctgcgtg cgcatgtgca gcgatgtgcg
     1861 gcaggtcggc gccatcggca ttgtgaaccg cggcatcgag agcgaagtcg gaacaagttt
     1921 cgcagaacct gtgctgcagt ccacctgcaa gttctgcggc gcgtgcgccg aagtatgccc
     1981 gaccggcgcg atccgcgacc ggatccccat gtgcgaaatt gaccgtgagg ccaagatcgt
     2041 gccgtgccgc gaggcctgcc ccgcgcacgt ggatgtcccg cgctatgttt atctgatttc
     2101 gcagggccgt tttgacgagg ccctggaagt gattcgcgag cggcttccgt tcccgtttgt
     2161 gcttggggcc gtctgcacgc acctgtgcga ggccgcctgc aagcagtgtc aggtgagcgg
     2221 tcagccggtc gccatccgcg agctgaagcg ctatgttgcc agccgcgaca gtggatcctg
     2281 gcgccgcaag ctgaaagtgg ttgctccgac cggcaagaaa atcgccgtga tcggctcggg
     2341 ccccgccggc ctttcatgcg cgtggtttct gcgccgggcg ggccatgaag tcaccgtgta
     2401 tgaggcgctc cccaggcccg gcggcatgct ccgggccagc attccgcgct accgcctgac
     2461 cgaagacgat ctggatcgcg aaatcaatgt catccgcgaa gccggcgtgg aaatcaaaac
     2521 cggcgttcgc gtggagtcgc ccgatgagct tctgaaaaac ggagccgatg ctgtattcat
     2581 tgccattggc gcggagaagg ggacgcgcat gacgtttgag ggatcgcccg aagatccgcg
     2641 catccgcgac ggactgacag tgctggagga catcaatgac ggccgcctca cgaagatgaa
     2701 aggcataatc gccgtggtgg gcggcggcaa tgtggcgctg gacgtggtac gcagcgcacg
     2761 ccggctgggc gcggagcgcg tgattcttct ctatcgccgg tcccggagtg aaatgcctgc
     2821 ttcgaaagaa gaagtggatt tcgcggaaga agagggtgtt gaatttcatt tccttgtaaa
     2881 tccggtgaaa gttttcggga agccggacaa actggaagtg gaatgcatcc gcatggagct
     2941 gggcgaaccc gatgccagcg gccgccgccg gccagtggcg gtcgaaggca gcgaattcag
     3001 gattgccgcc gaccagctca ttgccgccat cggtcaacag accgaggttc cggcttcttt
     3061 cggtatcgaa accgacaagt ggggctgcgt gaaggtggac aaggacacct atgcaacctc
     3121 gcgcaagggg gtctttgccg cgggcgatgc ggtgaccggt ccggcgtctg tgattgaagc
     3181 cattgatgcc gcgcgcaagg cggcatcgtc cattgacctc tatctcggcg gggacgggaa
     3241 cgttgagaag cccatcgcgc cgcctgagaa aatggatccg cggcttggat ccgaaaaagg
     3301 attttcaggc agaaagcgcc gtccggttcc cgttattccg gtggaggagc ggacgagcac
     3361 attcaacgaa gtggagaaaa cctattcgga agaggatgcc gtgaacgagg cctcgcgctg
     3421 tctgcgctgc aagctgcgcc ttgaaatcgg tcgcgccccg atgccgccgg agaataaggt
     3481 gaagatcaga tgaattcctg cgcatggaca tcaaccatat tgggaacgct gctgggcgtg
     3541 ttttatctat ggcttgtttt gaaacccgca tcgtggagaa acgcggccgc aaagtttcct
     3601 cgcagcgtgt gggcgggccg catcctgacg gccgtggcgg tgctgtgggt gtcatggctt
     3661 ctgttccaca tgaaccttgg ccggctctcg ttcctgaaat cggccgtgtg gtatctcgct
     3721 ccgatctgct atttgctgat cattttcttc atggatgatc tgctggcgcc gcgcgctctg
     3781 ggcggactga tgctgctggc ggccaatcca attttctatc atgtccgctg gcatgattcg
     3841 aatctgcgtc tgatcattgt ggtgctgggc tacatcatcg tcatcaaggg catgcttctg
     3901 gtcctgagcc cgttccagtt ccggcggctc gtcaaccgtt tcctgaacac ggacagccag
     3961 tgccgccgct ggggcggtgt cggcctcgct ttttcagcgt tccttctgat cctgggactc
     4021 ttcgtattct gattgtagaa aacggccaaa aaagcccgaa aaaaggccaa aaactgctca
     4081 aaaaggccga aaaacggcca aaaaacgcaa aaaacggcca aaaaatgcgg attttttgaa
     4141 atagtactaa attagtacta tttaaatgct cctgcggccg gcttctgaaa aatcgctgaa
     4201 aatgtcaaaa ataggccgaa aatcgccaaa aaatgcctat ttttggcgat ttttacaaaa
     4261 tttccaatga ttggaacttt ttacgggaag atttccaatg attggaactt tttgcaggct
     4321 cccggtcagg aaggccatgc ggctggatac ggtgcggtga ttacaatgac cgtgtgtttg
     4381 acgggatgga cgatttcaag ctggcggtgg tgcagcgcta tggtgttttc cgcaaagcgg
     4441 cttttcgcgc cgtatttggc gtcgcccgcg atcggacatc cttcggcggc aagctgggcg
     4501 cgaatctgat gatagcgtcc ggtctcaagc tgtatttcta tcagccacat tttccatgct
     4561 gtccccagca cgcgataatg aagaacagcc tgttttgcat ccgggtcggt ttcgcatccg
     4621 acaagagccc ggtgatctcc gtggatcagc cagtgggtga gagtgccggc tttcttcggc
     4681 agggcctgtt caatgagggc atgatagatt ttccgggttt tccggtcgcg gatctgctcg
     4741 ttcagccgcg acagggcttt gctggtgcgc gcgaagagca ccactccgct gacttcgcgg
     4801 tcaatgcgat gcacagcttc aaggaatacg gcaccgggct tgttgaattc ccgtttgacc
     4861 cattcccgcg cctgcgcctc gacgctgtcc tcggtcgtgc cgctgggctg ggtgaggagc
     4921 ccgccgggct tgctgaccgc cagcagatga ttgtcgctga aaagaatctg gtctttcgca
     4981 agcatgtcag ccgcaatttc cgggaagcca gcgcgcccag ttcccgctgg gcacttcaaa
     5041 gacatcccgg gcgcccgtga gcagcatttc cccgcacgac acggtccccg gtcccagcac
     5101 ctgcatcagc agatttctta acacgatggg cgagaagccc ggagtgtgcg atgtcagcag
     5161 aacgaacacc gggtcgggac tgagcacgga ccggacgttt tcgagtgtga tgagcaggtc
     5221 cttttcgatt ttatagagct cgcccctgtg accatgcccg aaggagggcg gatcaagcag
     5281 aatggcgtcg tatcgccgct cgcggcgcgc ctcccgcgcg aggaatttgt tgacgtcatc
     5341 cacaatccag cggacagggg cgtcctgcag gttgttcagg gaggcgtttt cgcgcgccca
     5401 ttccaccatg ccctttgatg cgtccaggtg gcagcaggcc gctccggctt tcgcggccgc
     5461 aagggtggct ccgcctgaat aggcgaacag gttcaggaac tgtatctggc gttttttctc
     5521 tccggcggcc tgccggacgg tttctgcgat ccagtcccag agcgcgcgtg tttcagggaa
     5581 tatccccaga tgcccgaagt cggtggacga cagcttcatc cggatgccgc tgacactgac
     5641 ggtccaggaa gccggcaggg cttcgcgccc tttccagttg aggccgcctg tgcggctgaa
     5701 tgacgcggtt gcggttttcc agagatgcgg cttttgcggc gcccatacgg cctgggagca
     5761 agggcgttcg agaatgatct taccgaagcg ctcgagtttg cacccgttcc cgctgtcgag
     5821 cagctcataa tcattggaac tcatagccgg gagattaagg caaagcaggg aaggggtcaa
     5881 gcggggcaga taaagagagg gcgtcctttc ggacgccctc tccaaagcgg caggaatcta
     5941 ttccccggcc ggggcttcca aaaccggaag ttccacgggg ccggaacctt cgggatagac
     6001 aatatcggct tccgtcttca gctgctcgat ataatccttg acggccgcct gctggctctg
     6061 catctgcagt ccttcgcgga tgcgctcgct gacttcctcc agcgggaggg tgcgtgccgg
     6121 ctcacgtttt tcaacaagaa tcagatgata gccgaactga gtctcgacca catcgcccac
     6181 cttgccgagt tcctgcgaga aggcggcctc ttcgaagggc ggcaccatct gtccgcgtcc
     6241 aaaagtgccc agattgcctc cacgggcctt gctggggcag tcagaatgct ctttcgccag
     6301 ttctgcgaaa tctgctccgc ccagaatctg ctgacgcagt tcttcaatct ttgccttttt
     6361 ggcggccttg gcatcctcgt catcctgcgc gtccgtcatg atcaggatat gactggcggt
     6421 gacactctcc ggcatttcaa aacgctggga attttcatcg taaaactcct tcactttttc
     6481 gtcggagatt tcgacttcgc cggctttcgc cttcaggagt ttgttgatcc gaatatccag
     6541 tacgagggat ttccggaaat cctcctcgga catcttgact tcctcaagct gctgttcgag
     6601 cgttacgccg ggctcaagca tgccggccac tttggcgcgg ccttcggcga tttcctcttc
     6661 ggtggccgtg atcttctcct catcaatggc cttctcaagc agcattttga ccacgagcgc
     6721 ctctgtgaca tcatcaacca tttgtccgcg catctgcatc attcgttcgg gcgggacgcg
     6781 gccctgtgcg cgcatcatca gtttcccgac ctccgtgtcc acttcaccct gcttgatttc
     6841 cgtgccgttg accgtcacag ccacggcgtt gggatcagtg gatggcatgg ccgcaggcgc
     6901 aaaaaggttg gcatctgccg caagcgactt ttcaaccgca ggagcctctt cctttttgga
     6961 acaaccgacc gacaacaggc cggcagaaag cgccatgatc agtacaacat agtatttccg
     7021 gttcatattc acctttcctt aatattcttc agtctgcccg gcagacatta ttttcccagc
     7081 aagtcgcgaa taatgggcct tttccccggc gcggtcaaca cttctcatcc ggggaccggc
     7141 cacaaaaagc ttcccgtatt cagaaaatgc acagtctgtt ggacgctctg tctggatttt
     7201 tgttcaacag attcttctgt attgaaggca ttatgaggcg tcagtacggc atttggacgg
     7261 tttccaatct cgcgcagcac tcgaacgagg gggtcatcgc tcggctggcc tgaacgcagg
     7321 agcagggaga tgcggggttc ctcggggtat acatccaggg ccaccccccc cgcaatcccc
     7381 tcgtccagaa ggcgcagcag gtcaactgga ggggacattt ccccgcgggc gatgttcaca
     7441 aaaacaacgc cgggtcggca ctggctcagc cgctcatagc tgaaatagcc atggttttcg
     7501 gccgtcagat tcatggagca aacgaccaca tctgcctggc gtagcccctc gtcaatctgc
     7561 atgtagctta tatcggagtg cttttcgacg atgtccacgc cataaacccg catattcagg
     7621 gcctggccga tgcgggcaat ttcgtatccg atgcgcccga ccccggtcac aagcagggtt
     7681 ttccccgcgc actcgcgtcc ggtgattccg tcgcgaagga aaactggaaa ctgccgctgc
     7741 tgttgcggga gcttccgcag caaactcatc cagagcaggg cggcctgttc ggccaccgcc
     7801 cgcacgcaat attcaggcag ataaccggct ttgatcccgc agggattctt cttaagccag
     7861 tcggcaacat ggtcgtatcc ggcactccgg gaaaggattc catgaagcga ccgggtccat
     7921 gccgccggga caacagactg ggttcgaatg ctgatcaatg cagcgggcgg cacggcatgg
     7981 cccgcctctt ggatgctctt ccatgtgaaa tccgcgctga tttccggcgg caggcaggcc
     8041 cggagcgaat gttcctcctc cgcgaatgcc tcatagaaaa aaacagacag agaatttttc
     8101 ataatatgct tcgattcacc gtccagacgt ttacatgctc cacgtaaata atcaaaactt
     8161 ttcagcagaa agacctttta ttcccattga cgattttgta ggttttacaa gcaacaggat
     8221 tatgaagtgc gtcagtatca tgacgatctg aatcaacacg agagaggaag ctatggcaga
     8281 aaacgaagtc gctgatctgc aggtgaaaga ggaggctgct gttacacggg gcgcacgtcc
     8341 caatacagat tgtaattttg ttgtacagtt gcttgttggc gccatcggca ccggcattgt
     8401 ggttgccgct gtgcttcccc tgaaagggaa ggggccgctg gcaagttaca tgcacggcat
     8461 tgtgatggag cgcggtccgg ttcagtattt cgaactgttc atgttcttca tgacagccgc
     8521 attccttttc ctgaaatcca ggattgtccg cgcccagttc cgagtgctca gtcagggtcc
     8581 ggtcaacccc accattgatc tgacggatga cgatcagatt caggatttgc gcaatgagat
     8641 tgtcaaccat gaggccttca gctggagcat tcttctgaac cgcattgaac ggatcatcgg
     8701 actgtggctg ggatccaagg atatcagccg tgtttccacc tggacctcca ccgagtcgtc
     8761 gaatgatcag aacacggctg aaagcaccta ctcccttgtg aacgtgcttg tctgggctat
     8821 tccgatcatg ggttttatcg gaacggtgca gggtctgggc gcggccgtgt cgggtttctc
     8881 cgcgtttctt gcaggcgccg ccgagctgag cgccattaag gacgccatcg gaaacgttac
     8941 gatcggtctg ggtgtggcgt tcgataccac actgctggcc cttattcttg tgaccttcct
     9001 gatgttcccg ctggcctccg tgcagcgcaa agagcagacg ctgttcggtg aaatcgacaa
     9061 ctatatcgat gacaccatgc tttcgcgctt cccgtcacag gagcagcagc ccattgtgat
     9121 cgagaatctg gaagattcca ttgaagcggc cttccggcgg tacatccccg atcctgaccg
     9181 ctacgacgaa gtcttcaccc gctcgattga caaggcggca aacatggtcg agggacggtt
     9241 ctccggcctg gccaaaaatt atgagggcac gctgaatgcg ctggcagacc gcctgcagag
     9301 cagtgtggcc ggcgcgggcg atgccctgca gggcgccatt gcgggcgtgg ttgcgaacat
     9361 tgacaagtct tcgcaaaaat cgactgaggc ggcggagcag ctctcaacca ggattgccga
     9421 ggttggcaaa ctggcctcca gtgtgcagga gatgctgcag ctggaagctt ccatcgaaaa
     9481 gagcttgaag ggaatttccg cctctgacga gttccagcag acactcaagg aaatccgtga
     9541 acacctggca gccacagatc agttctgcaa gcagttgaac aagcctcggg tgattacttt
     9601 caaggaagaa attgccggct gaaaccggtc cgtggccggt ccgcaatagg gcatttatgg
     9661 gtataagaaa caacagcagc ggctgcagcg cggacgcgtt ggcgcgcgtc ctcttgctgg
     9721 tgcttctgtg ctgtgcgggc ctgtttccgg cttatgccta tgaaggatcc ctccccgcaa
     9781 aagtggcggt cagcgggctg cagggtccgg cggggatgac ggttcatccc gagaccggtg
     9841 atatttacgt ggcggagaaa acggccggtc gcatatctgt aatccggagc ggggcggcct
     9901 ctccggtgat cagtacggga tggtcgcttg atcccaacat ccccttctgg gcagtcagcc
     9961 gggaattgtc aaaggaagcc ctcgtcaaac ccgctttgaa taatcccggc agcgtggcca
    10021 ttgcaaccaa cgggaccctt tatgtgtgtg aaagcacccc cagcggccgc ctgcttgaat
    10081 tcgctccgga tgaacagggg aactatacaa aagggaaaat cataccgatt ccatggattg
    10141 accggccctt ctcctggact gatgtcaaag ttgcccggga tggccggctt tttctggttg
    10201 gcggagtggc cgaagctgat gttttgcact ttggaactgt gctgatgagg gatgagcagg
    10261 gagactggtg ggtggtggat tatggcccat tcatcaactt cagcagcgtt tatctttcgc
    10321 gcaatgaaga tatcgtcatc gtctgcgaac gcgacctcgg cggggtggtg tggtgggatg
    10381 ctgtccggca tctcccgatt ggaactcttg aggaaagcgg caagaacata tccgcggatg
    10441 ccgtctcatt gatggtggat ggggcctttg tggtggggca gaaccttgcg gccggcgggg
    10501 cgcgtgccgt ccgggtggac ccgctgactg gtgaacagaa ggatctgata agcggactcg
    10561 accgaatcgg aggactgctt ctgtccagtg cgaagaacag cctctatgtt tctgatgaag
    10621 ccaaaggcgt gatcgtggaa gcctccgtat cgcccccctc ggaaggcatg gaatatctgc
    10681 tgcagcgctc cctggacggg taccagatgg gagaaggctt tactcctcgt caggctcccg
    10741 gtttcctgaa aaacttcttc tctcaggttg gttcagccca gacaggggca accggggagg
    10801 aagaggggct ggatgcggtc acggaccgtt tgaccatgtc gttttctttg agggaatttg
    10861 ccgccaaaat cccgctgatt gccggaaaga tcgagacccg atcgctggag gaggcatccg
    10921 cttcagtcag tgaggatccc ctgaccctta ttgaatttgt gattttcttc cccggcaaaa
    10981 ccatagccag cggcgatttg gccacaccga gccttgctta ttttgccgcc cagcgaaaaa
    11041 gcggcaaggt ggagcgtaca cgtgaattgt tttcgggcct gaacatccgc cagcgcaaat
    11101 cggggggaga atggatggcc ggcggggaca acgcccgcat tatggttccg gtttcttctg
    11161 ttggacttga gaaaagcgcg acagggatgg atcttaatct ggcatttctg ggtttgggca
    11221 tatacagcga ctactatttg cgcctaacct cgggacagga aaacaccgga gagatcgtgg
    11281 tggaggggct ggacggaact cgcgaagtgt atcgcgcggt tttcaccgaa atggccaaag
    11341 tgggcatggg cgccgaggtc ggatctgaaa tcaagaacct ggtcgtggcg ggtttcgacc
    11401 cgatgcagca ggattcgggc atggggtggc tgaatatcgg ccgcagtccc gtgaatgtgg
    11461 cggtcgcaac aagcatgagc gaactgcatg agtttgcagg catggacagt aaaatcaccg
    11521 caatgatgaa gaagaaggat cgtgagatta tttcaacgcg gcccgaagat gtggttgaag
    11581 gagcctcccc ccagccggtt gctccagtcg aagccgggga cagtccggag acgcagccgg
    11641 cagtttcgaa tgaggcggat gccgccgcgg ttccctcatc ggcagaggag gccgcgccgt
    11701 gatgaaggag gggttgaact aaatggccgt caaaatggat ctatcctctt tcatgtgcat
    11761 cctgatcatg gcgatcggct gcctggtggt tattctcgtt gtaaacgtga tcatcattgt
    11821 cagcaacccg gagaatgtgc agatcacatc cctcttccgc ggggcctatg aagactatga
    11881 ttctgaagaa ggccctggag ccaccatcgg ccagcccaaa tacggaaacc tgttcaagga
    11941 tcctttctac gtcgatgttc acagtgatca gatcattgtt tatccggaaa aaaatgtggt
    12001 gacccttcgg caactggaag ccggaaacaa tgcgtttgag gatctcctca acaaaattga
    12061 gccgatgaag gataaaacct atgtgatcat ggtgcttcgg ccaaaatccg cgcgcatgag
    12121 ccggctggtg cggcgtgcca tccgtcatcg cggcattgat gtggggcagg agctttttga
    12181 ggcgggccgc aaaatcccgg aaattaaatc gaccgcggaa atcaacgcgg aattttcagg
    12241 gataaccaat gctccggctg aagacgcagg tgcggaaatg gaagccggcg cagaagccgc
    12301 tgcgcagtaa caggacttta gtatggcaag gaaacgaaaa aaaaaggcag cgtcaatcaa
    12361 ccttgactcg tttctggaca tcatgacatg tcttgtgggc gtgctcgttc tgatcattat
    12421 tctgaccagt attgacgcgt cgcagaccaa gcttttgatt cccacgccca tggctcatga
    12481 aaccaccaag cgtccggtgt ttattgagtg ccgcggcgac atgctttatc cgattgatct
    12541 gcccgaaatc cggcggaaga tggacgaaga attgagccgg atcgagaaag agtcactggg
    12601 caacaaagaa aaaatgatcc agatgatgag tcaggccatg attgaaacgg attactacaa
    12661 ggtggacttg tcctatgcgc tggccggaca gaatctgctg atcccgaaac ccgaagccgc
    12721 tggtctgact ctggataaca tcaccgcttc aacgatgtca atgaccgctc tcccgggctg
    12781 gctggacacg ctcgtcaagc agctgaatcc ggaagaggat attgtcacat tcctcgtgcg
    12841 agacgacagt ttccgggttt tcaagaaagc gcgcggtatc gcatggcttc tgaatgttga
    12901 agtctcctat gaactgctgg atatgaacga ccccatcaaa ctgggtttgg gcggggaagt
    12961 ctcactggct cagtaactct cccatgccgg aaacttactc tgaatgcatc cagtgcggcg
    13021 attgctgccg gtggagcggt ttcgtttacc tgaatgagga cgatatccgc cttgcggccc
    13081 gcttcctgaa tatggaggaa tctgcgttta tccagaaata tgccgtactg ggtcccaacc
    13141 ggcatcagct ctgcctgatc cccggtgccg ggcgtgattg cgtattcctt tgtgggaaca
    13201 actgccgcat ttatcccgcc cgcccgcagc aatgccgcga tttcccgctg cgctggggaa
    13261 cctgtcccca tcggcaggcc tgaagcctgt tttatggttt gtcatacgcc tttttttagg
    13321 gcatcataca cggcggttta ttgaagaagg agcagatatt atggcggtca tggaattgga
    13381 agacatcctt gaaattctgc cgcaccggta cccgatgctg ctggtggaca gagtgatcga
    13441 atgcgatttt gaaaaacgca ttgtgggcat caagaacctc acttttaacg agatattctt
    13501 tcaggggcat ttcccgggcc atccggtgat gccgggggtg cttcagattg aggccatggc
    13561 ccaggtcggg ggcattctga tcaacaagat cgccgccgcg gagaaagccg tggcctattt
    13621 catggccatt gacaacgcaa agtttcggca gatgatccgg cccggagatc agatgcgcat
    13681 ggagctggaa atcgttaaaa tgaggaaaac ggtttgtgtt atgcacggga aggcctttgt
    13741 ggacgggaaa ctggcggttg aggcagacat gacatttttc cggagctgac tgatttatga
    13801 ccagtattca tcagaccgcc attgtggacc ccggggcaga gttgggggag cgggtttcta
    13861 tcgggcccta ctgcgtggtg ggcgctgatg tccggattgg cgatgacgcc gagttgatgt
    13921 cgcatgtggt ggttggagga cacacgacga taggctgtca gtgccgcatc ttcccttttg
    13981 ctagcattgg tcagcaaacc caggatttaa agtttgacgg caggatatcc tatgtggaga
    14041 tcggcagccg caccacgatt cgcgaatacg ttactgtgaa tctggccacc agcgagggcg
    14101 acaccacccg catcgggtcc ggctgtctcc tgatggccta ttcccatgtg gcccatgact
    14161 gtcaggtcgg cgattcggtg atcattgcca actgcgggac actggccggc catgtaatcg
    14221 tggaggacca ggttattgtc ggcggactga gcggcattca tcagtttgtg cggatcggac
    14281 gtctttccat tattggcggc tgttccaaag ttacacagga tgtgcctccc tttatgatgg
    14341 cggacggcca cccggtcagc atccatggcc tgaacagcgt ggggctgaaa cgccgcgggg
    14401 tctcggccgc tgcgcgcaag cagctgaaag acgccttcaa gctgatttat cgccaggatc
    14461 tcctgaccac gcaggcggtg gagcagatac agtcctggcc ggaaaagaca cctgaactcg
    14521 agcatctcat cgcgtttgtt aaatcgtcgg accgggggat tgtcaaatga tctggcgtcg
    14581 gcggcttcct ttaatgcttc tttttctgct ggttccctgt gtgcttctgc ccagcctgct
    14641 cccggcggag cagaacggtg gccggtcgca ggttaatttt aattttgacc gggtggatat
    14701 ccgtctgctg gtcaagctgg tgggcgaaat caccggcatg cagtttgtcg tggatgacag
    14761 tgtgaaggcg gaagtgaccg tggttacgcc cgcccagatc cctgcagatg aagtttatcc
    14821 gctgttcgtt tcgattcttg agtcatcggg ttattcaatt atccgcaaag gaacggtttc
    14881 gcatattgtg ccgctggctt cacgggaggt gccttctgct ccagtgcgca ccggcgacac
    14941 ctcggatgcc gtgggcatta tcaccaaagt catcaggctg cagtatatcg acgccgttga
    15001 aatgcgcgcc gttctgctgc ccatggtcag cggagggaaa accggaagtc tcagcgcatt
    15061 tggcccgacc aatcacctgc tgatcaccga taccgctgag aacatcgcgc gaatcgagga
    15121 aatcatagcc gaactggaca aaaagggatc caaccggatc gccgaggtcg ttcccctgaa
    15181 aaatgcctcc gcagccgaag tggcggcaca gatcaacatg gcgattgaag gcggagaaag
    15241 cgcggggcgc aaggtcagcc gccatatgca gcaggtggct gacggtcgcg gcgccatgcc
    15301 cacggaaatg acggtggtgc cggttaccca tgccaacagt ctgcttttgg tgggcactcc
    15361 ggccaatatt gaggaaatca agcggatgct ggagcaaatc gacacggaga gccttgctgc
    15421 gcgcggtcct ctcagcgtga tctttctcaa atacctgtcc gccgaggccg ccgccaaaag
    15481 cctgaatgcc ctgctggaga aatcggccga gaaggaccag aagcggagca tcgcgatcga
    15541 gccggacatc gccaacaatg ctttgctgct gcatggcagc ccgcgcgact gcgactgggt
    15601 gcgggaactg gttgaccagc tggatcaggt tccgcagcag gtgcttgtcg aaatcctgat
    15661 tgccgaagtg tctgtgggga aaaatctgga tctgggcgtc gagtggtcca ccatagatgt
    15721 ggcggctgag ggccgcacga cggtgctggg ccgcagccgg cccggggaaa ccgacacact
    15781 gatggattat atcacgcagg gcgtgctccc gcaggggctc gcggtgggcg tggcgcgcgg
    15841 gacctatacg gatgcctccg gcgccgtgca gcccagcatc ccggtgctgc tgcgcgccct
    15901 ggcgcagaat cgggacatca agattctctc caatattcct ctctgggctc agaacaacac
    15961 cgaggccagt gtcagcgttg tggaaaacat tcccattctt aaatccacca ttgagggcgg
    16021 cgcggggacg gcgagggata ccattcagaa catcgatcgc attgatgtcg gcatcaagct
    16081 gaaactgacg ccgcatgtga acccggaccg cgaggtcacc attgagctga atcccagcat
    16141 cgaggccatc atcgacaccg ccgcatcagc cgaacttccc ttcaccccga cgatcaccaa
    16201 gcgcgaggtg gccaccaccg tcaccgtgaa ggataaaacg actattgcca tatcgggtct
    16261 gatccgcgac gatcagatca ccactgttta caaggtcccc ctgctgggag acattcctct
    16321 gctggggatg cttttccgca gcacgtcccg gcgcgcagag cgcaccaacc ttctgatttt
    16381 tgttacgccc cacattgtga ccgaagcgga gtccgcgctg gccgcccgca ggtcatggga
    16441 ggaccggtcc gaagccggaa gcctggccac aaactacatg tccaccattg aactgaggga
    16501 gtaaaatgcg gccgctgggg cagatactca ttgagcgcgg aatcatcagt tcctcgcagc
    16561 ttgaggaagc tcttgcgcgg cagcaggccc tgcccgggcg ccgtatcggc gaaatactga
    16621 tcgaggcggg atgggtttct ccggctgatg tgctcaaggc gcttggcgtt caataccagt
    16681 gtgaagtgct ggatataatc ccgcccgaga tgcttgactc tgaacttgtg agcagcctgc
    16741 ctgtggaatg ggcgcgctcg cacgagatgc ttccggtccg tcatcgggaa cagccggctg
    16801 tgctgacggc cgatcccacg cggatcaacg agcaggatga tttgtctctg ctcacgggat
    16861 gtccgctgat tcctgtgctg gcccttcccg ggttgatccg cgaagccatc gaaaactgtt
    16921 acttcatgcg ccgcgagaca gcctctgaag tcatggccgg tcttcccgcg ccggaggcgg
    16981 tccagccgtc ggcggcagca ggagccacgg atctgctggc ggtcgcaacg gatgcgccgg
    17041 tcacccgcct ggtcaatctg atcctgctgg aggcggtccg ccgccgtgct tcggacatac
    17101 atattgagcc gctggaagat tcacttcgcg tgagataccg gatcgacggc atgctttatg
    17161 aacagtcggc tcctccgcgg cattttgagg cggcgctggt ctcgcgcctg aaggtcatgg
    17221 ggcaccttga tatcgccgag aaacgccttc cgcaggacgg catggcccgg gtcagcgtgg
    17281 gtgagcgcga cattgacatc cggatttcaa ccattccggt ggccgagggg gaaagaatcg
    17341 ttctgcgcct gctcaaccgc gagtccaccc tgctgccgct cggcagactc ggcatgcctg
    17401 atcatgtcct ccggccattt ggcatgctcc tcaagctgaa tcatggggcc atctgggtca
    17461 ccggcccgac gggaagcggg aaaaccacca cactgtatgc ggcgctgcag gagctcgaca
    17521 gcgagcattt gaatattctg accatcgaag accccgtgga atatcagctc gcgcatatca
    17581 gtcagatatc cgtgaaacca aaaatcgggc tgacctttgc gcaggggctg cgtcatattc
    17641 tgcgccagga tccggatatt atccttgtcg gggagacccg ggacctggaa accgcggaga
    17701 ttgccgtgcg ggcgtcgctg accggtcatc tcgttttcag cacgctgcac accaatgatg
    17761 ccattggggc cattgttcgc cttgtggaca tggggattcc ccgctatctc gtgtcagcgg
    17821 cgaccagcgg ggttctggga cagcgcctga ttcggactct ctgtcctgaa tgcagaaagc
    17881 cgatggagct gtccgcggaa gatgccgcgg gtctgggacc ctgtgcgcag cgtttgaagg
    17941 gacagcctgt gtatgatgcc gggaactgca gcgcctgcat gtcgggctat tccggccgca
    18001 cggggattta tgaactgctg atgatggatg acgccattcg ggatgccatc cggcatgagg
    18061 ccgatctgat ggctctcaac cgtgccgtgg cgggcagggg gttctctcct ctgacggaag
    18121 atgcagtgga aaagattcag cagggcatca cttcacctgc cgaggtgctg cgggtgctcg
    18181 gcagattccc gtaaccgtcc tatgaaaacc tatgaataca gggggtatca gacttccggg
    18241 catgccatca ggggcctgat cgaagcctcg ggcttgaagg atgcccgcga acggcttgca
    18301 gagggcggta ttctggtgga aagaatatcc tctgcttctg tccgccgcag aactcttcca
    18361 tttcggcgca gccggggcct tgattccgaa acccgctcat ctgtatatcg ggaactctct
    18421 tcgatgatga gggccggcct gacgctgacc cagtcgctga atctgctgat tgaggccatg
    18481 gaagaggccc cggccacacg tgtactgctt gccggcatgc gtgatcgggt gcaggagggg
    18541 gcctctttgg cgcaggcgct tgccgaggtg agcgggggaa ctcatgaaat ggagctggcg
    18601 gctgttcgtg ccggcgaacg tgcgggaaat cttgatgtgg tcctcgagcg actggccgcc
    18661 ttccttgagg agcagagaga gatccgcgag cgtgtgcaga ctgcgctgat ctatccctcc
    18721 attgtcctgg ggatggcggt tcttatcgcg ctcctgatgt tcggagtgat gatcccgcgg
    18781 tttgcatcct tgtttgaaga atctcaaatc ccgctgccgc ctgtgacgcg gtatattctt
    18841 tcagccggac aattcctgaa ataccttctg tttgcgctga ttgcgtgtgg ggccgcctcc
    18901 actggattta ttgccctgaa gtcgtctgat cctcagttcc gcatcctact ggaccgcttc
    18961 tggtttaatc tcccgctatg
//
LOCUS       DAOQWR010000039        18944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3285_length_18944_cov_6.157076, whole genome shotgun sequence.
ACCESSION   DAOQWR010000039 DAOQWR010000000
VERSION     DAOQWR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18944
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_18944_cov_6.157076"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PKK36_04770"
     CDS             complement(<1..715)
                     /locus_tag="PKK36_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93907.1"
                     /translation="MHHPFSALQSAEPDGNRRGRSLSPVKIRSNRPKKQNNADFWPKI
                     KVFCHFLTFFDVFGPFLTIFRQKIAFLGPSSIIAEILEIQINSRREMINIAPPSRRFG
                     VRWLDAAFTEFTDGFSCSSFHDHDSRGRLYLQGFWCSSRSESGICFRMRLTEALLHRR
                     RDHADSPGNYVPSPCTWLSPVLYFFPAASSISTNVPTKIVEELKNGQKVPQNVKKWPK
                     NAQKRLKTAKNSKKWPKIEN"
     gene            911..1120
                     /gene="rpmE"
                     /locus_tag="PKK36_04775"
     CDS             911..1120
                     /gene="rpmE"
                     /locus_tag="PKK36_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNR93908.1"
                     /translation="MKKDIHPTYEDATVTCACGNVIHTRSTRKEMHVNSCSACHPFFT
                     GQAKYIDTEGRVERFRKRYQTEKKS"
     gene            1190..2275
                     /gene="prfA"
                     /locus_tag="PKK36_04780"
     CDS             1190..2275
                     /gene="prfA"
                     /locus_tag="PKK36_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNR93909.1"
                     /translation="MITRDYIQKLRDRLAAVEQKLSEPGVTARQEAFQKLVAEHKHLK
                     KTLTAAERFFRLEDEAASTRALLEDSGLDSDMKQMAEDDLARIEEEKPKAERDLMVAL
                     LPPRPEDSRNTIIEIRAGTGGEEAALFAGDLFRMYTHFAEEQGWKVNLMDASPSAVGG
                     YKEIIFAVEGEDVYRVLRYESGVHRVQRVPETEASGRIHTSAATVAVLPEAEDVDHID
                     IKPEEIRIDIFRSSGPGGQSVNTTDSAVRITHLPTGLIVQSQDERSQHRNREKAMRVL
                     KARLLDKYQQEEAAKYASQRKSQVGSGDRSERIRTYNFPQNRLTDHRINLTVYSLDRV
                     IEGDIRGLTDALYEFDVAERLEKELKA"
     gene            2253..3125
                     /gene="prmC"
                     /locus_tag="PKK36_04785"
     CDS             2253..3125
                     /gene="prmC"
                     /locus_tag="PKK36_04785"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNR93910.1"
                     /translation="MKKNSKPETVRELLRETEDLLAEHGLNEARRMTELLVSHYCGCR
                     PLQLYTRMGDPAAAALRRAVEQGRERLLAHEPLQYVTGETDFFNCTLKTDSRALIPRP
                     ETEELVQLVLDTAELWKIPLPALADVGTGSGCIPIALCKARPQGRFTGIDRSGEALAL
                     ARENAERNGLGELIRWVRNDLLAGFPPGSLDAVTANLPYIPSGEWAELPPHIRRHEPQ
                     AALAGGPDGLDVIRRLIPQAGAVLKNPGWIFLEIGHSQGHDVADMLKQNGFEEVAVKR
                     DLSGLERMVTGRKA"
     gene            3122..3532
                     /locus_tag="PKK36_04790"
     CDS             3122..3532
                     /locus_tag="PKK36_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93911.1"
                     /translation="MSILKAKRKFKIPQQAILGPVIIVVFVALVMQANWTRRNQRLFR
                     GMMSGLERLPCQYCRGTGTIMEEDEAERQTALHCPVCYGVGFRFIRRLDENDKLCPAC
                     GGMGRLQDPDTGHYRTCNRCDGRGLIQAPPGDRR"
     gene            3532..4860
                     /gene="ffh"
                     /locus_tag="PKK36_04795"
     CDS             3532..4860
                     /gene="ffh"
                     /locus_tag="PKK36_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNR93912.1"
                     /translation="MFDSLSASLQKVFKNLRGYGRLSEKNIQEALREVRLALLEADVN
                     FRVVKDFIAQVREKALGRAVLDSVTPGQQVIRVVHDELVNLLGGEPAGFTLAPSPAVI
                     LMLGLHGAGKTTTCGKLALKWKQEGKRVLLAACDIRRPAAVDQLRILAEQTGVEIVTP
                     QPGESVPGIAKRAAAEAARRKADVLILDTGGRFQIDDELVEEVREVHREIRPANTILV
                     LDAATGQEAVNVAQAFHDRVGLTGLVLTKLDGDARGGAVLSVRHVTGCPVILAGTGEK
                     MDRLEPFYPERMASRILGMGDVLSLVEKAQQQVDAGEMKAMEERLRKNTFSLDDFLSQ
                     IQQLKKMGPLGDLLEMMPGGGQIPADVKQQMAASSGDGLKVTEAIIRSMTPKEREHPE
                     IISASRRRRIAAGSGTRVQDVNELLKRFEGAKKMARTLKKSQKRLLRFGK"
     gene            4938..5237
                     /gene="rpsP"
                     /locus_tag="PKK36_04800"
     CDS             4938..5237
                     /gene="rpsP"
                     /locus_tag="PKK36_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNR93913.1"
                     /translation="MAVKIRLRRMGEKNLPFFRVVVTDTRYPQTGRFLETIGWYDPKK
                     KAGSDIQMDMDRVNYWVGQGAQMSVTVKDLVKKVSRTTPPKAPRKAKAEAEAVEA"
     gene            5244..5474
                     /locus_tag="PKK36_04805"
     CDS             5244..5474
                     /locus_tag="PKK36_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR93914.1"
                     /translation="MKELIEYIVRILVDHPEQVQITEVDGEKTVIFELRCNKEDIGKI
                     IGKSGKTVGAIRTLLSTAAAKDGRRALLEIVD"
     gene            5481..6164
                     /gene="trmD"
                     /locus_tag="PKK36_04810"
     CDS             5481..6164
                     /gene="trmD"
                     /locus_tag="PKK36_04810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR93915.1"
                     /translation="MPACLKIDVISIFPEILDAYLGESMMKRAAQAGLVSFRTIDLRN
                     HTHDVHRSVDDRPYGGGPGMVMRPEPLFEAVEAVRTPESRVILMSPQGRPFTQARAGE
                     LSGEKHLILVCGHYEGVDERVRGALVDEEISIGDYVLTNGILPAAVVIDALVRLIPGV
                     LGAGEEAVEKESFQDGLLEHPQYTRPAEFRGMKVPEILLSGNHEEIARWRRDQSLRET
                     RKNRPDLLS"
     gene            6282..6635
                     /gene="rplS"
                     /locus_tag="PKK36_04815"
     CDS             6282..6635
                     /gene="rplS"
                     /locus_tag="PKK36_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNR93916.1"
                     /translation="MSKILEKIDAEQRKPADQIPDFKIGDTVDVHVRIREGDKERIQV
                     YSGVVISRKGHGARENFTVRRISYGEGVERVFPIHSPNLAKIELNRRGKIRRAKLYYL
                     RELAGKKSRIKESRD"
     gene            6688..7278
                     /locus_tag="PKK36_04820"
     CDS             6688..7278
                     /locus_tag="PKK36_04820"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNR93917.1"
                     /translation="MAGIDEAGRGPWAGPVVAAAVCILPDFLRQEEHGVFEGLTDSKK
                     LSAARRDHFFEVLTTCGAVRCGIGRAEADEIDSINILQATHLAMRRAFDALRPAPDFA
                     LVDGNRLPPLPCAALAVTGGDGKSLSIAAASILAKVTRDRLLQEADERYPGYGFARHK
                     GYGTAGHSAALRKLGPSPFHRMTFRPVRECLERNAR"
     gene            7275..7709
                     /locus_tag="PKK36_04825"
     CDS             7275..7709
                     /locus_tag="PKK36_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNR93918.1"
                     /translation="MIAWLQRLLRRKKSLPEHLKRGLWGEKVAEDALKKKGYAILGRR
                     VRMGKRGELDLVAKQGETLVFVEVKTRAGEQFGRPFGAIKSHKKKMLMNAAKCYLLKL
                     KERPRYFRFDAVEVIGREGAGEPVVRHIESAIKITRKYRIQW"
     gene            7721..8674
                     /locus_tag="PKK36_04830"
     CDS             7721..8674
                     /locus_tag="PKK36_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNR93919.1"
                     /translation="MKKTITIVFLLAASAACSGIEDLDFTEMATAGLEWFEENVPESL
                     HARIGEVDWEYYTEKLLGHLQEYSWEDMAGCEEQAQTLLAFLNQYERLEPLAVWLETR
                     MDYFAMADEFLQQLRELPPEPEVRPRPVPGPAPLPPPAPRRRPVYPPQKKWEAKLKTR
                     PKPARADGMVPELKTIFEREGVPGEWVWIAEVESSFNPSARSPAGAAGLFQFMPATAR
                     RFGLKTSPQDERLDPRKSAAAAARYLGVLHRRFDSWPLALAAYNAGEGRVGGLLKKHG
                     AGDFDGIAEKLPLETRLYVPKVLATVSLREGVNAEHLPPPS"
     gene            8728..10926
                     /locus_tag="PKK36_04835"
     CDS             8728..10926
                     /locus_tag="PKK36_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR93920.1"
                     /translation="MRFRRAILILPVAVLGVLMVSSRAEPEGARPKVGLVLSGGGAKG
                     FAHIGVLKQLEEMHIPIDCIAGTSMGAIMGGLYASGMSPREMEQIFTGLDWWDILKDQ
                     TQRKDLAFRRKKDDQRYLGNIELGFKHGRFRLPRAMAAGQKLNNLLVTLTRDARRNFD
                     DLNIPFRAVATDLSSGDRIVMGDGSLARAMRASMAIPGVFSPVEIDGMTLVDGGIADN
                     IPVDLAREMGADIIIAVDLGAMDIASTRRSQGDIAELLAHTYQLIRRPQQEEQMRDAD
                     IAVTPDTSMFSSSDFHKARTIIGTGRRAARAVEEQLRPLSISEEEYAEWLRRHRRAAD
                     DTPVIRSIAITGEKHVDERIIARQIKTRLNRPLDYELLEDDLSRIHGLGYFESAMYGI
                     SGEGGQRDMTIEVSEKPWGPGYVRFGLKLDADSEENSSWSVLANYTRRPLNRLGGEFL
                     LDLQAGEKRRIMAELYQPLDFRGAFFLSPSAEYSSSMMDVYDGDRKAAEYDVEEFYGA
                     LNAGVQIGKYGQIQLGMVAGRARATVDVGDPDLENIDQDAGGWTGHIVLDRRDSDVFA
                     REGFLVVLQGFSGDEMLGHEDSYEKVSWLSQHYMTLGRHTMFAHLELGTSLESELPLY
                     DEFALGGLYSMSGYADGQLRGQYLGVGRLGYMFRISQLPPDLGRGVYIGAWGEAGNVW
                     QDEGDIDSDSLLYGATVGLGADTFLGPLYFAYGRSEHGRDRFYFSLGNNF"
     gene            11085..11567
                     /locus_tag="PKK36_04840"
     CDS             11085..11567
                     /locus_tag="PKK36_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR93921.1"
                     /translation="MVVIKTSKGDIKVELNEKKAPGTVKNFLAYVDAGHYDGTIFHRV
                     IDGFMIQGGGFTKDMRQKPTRAPIENEAANGLGNERGTIAMARTSDIHSATSQFFINV
                     VNNAFLNHRDKSAQGYGYCVFGRVAEGMDVVDAIKSVRTAAQDVPVETVEIISITRAE
                     "
     gene            complement(11580..12419)
                     /locus_tag="PKK36_04845"
     CDS             complement(11580..12419)
                     /locus_tag="PKK36_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNR93922.1"
                     /translation="MKPVINLLVQLQELAEARAQQEAVVSNPQLTELDASIEALRKKL
                     PPDLSSKFTKLQQKGHVAIVPIAGGRCSACAMQLPSSLIQLVAAGTQVSTCPTCARIL
                     YMPPETLPQRISRRTQRFEAPKIGIARFSALNLMMPHIEAATRDEAIEEMSMKLEAEQ
                     FVDNGRKLRDAAIARESIISTAVEHGIAFPHVRGIEGGGLTLALATSKKGIRCEQGGK
                     NLTRIIFFLLIPTGASAFYLKLLAGLTESFQKKENRDKLLDADTPEKLWKALAATTRS
                     TVK"
     gene            12632..13078
                     /locus_tag="PKK36_04850"
     CDS             12632..13078
                     /locus_tag="PKK36_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002705684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNR93923.1"
                     /translation="MDVKKAISTDTVFQDLKSTTKTGVIEEILDLLIAAGKIRKENRA
                     EVLNALLVRENKMSTGMQHGIAIPHAKTDAVEDLVAALAIKKEGIDFDALDGKPSHIF
                     IMTISPVKRTGPHIQFLAEISRKLNQPSIREKLLLTQTKDEILQLL"
     gene            13084..14748
                     /locus_tag="PKK36_04855"
     CDS             13084..14748
                     /locus_tag="PKK36_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNR93924.1"
                     /translation="METILAIPGLLLASVAEHAVERDMTHLMTQYILQLAVIMLAARM
                     GGFLVKRYLNGPAALGELAAGMAIGPCALGAWHIPGWGALFPPVAGIFPVSTELYALA
                     TLASIVLLFLSGLETDLRVFLHYSVAGIVVGTGGLLASFFLGAWCALLFNVADSLMDP
                     SALFMGAMATATSVSLPARILSEKRKMASPEGVTILAAAVFDDVLGIIALAIIVGMSK
                     TMASGAVMDWGKISVVAVKSIGFWLILMAAGLLLAKHIARVLKLLRSPNTIAAICFGL
                     ALLLAGISEMAGLAMIIGAYIMGLSLSGTDLVYELQEQLQGVYDFIIPVFFCVMGMLV
                     NFAAMKGLIFFGLVYTVMCIISKVAGCGIPALFMKFTPRGAFRVGAGMVPRAEVTLIV
                     AGIGLSAGYIGPNLFGVAVMMAMLTTVAAPPMILSAFRGGSGVRGEETAQEQELSTAV
                     LTFPSADMAEFICARITRAFRNEEFFVHMLDSDVPTYQIRKEDMAFSIVQDDVHITIS
                     MPLQYEHVGRLIVLEEVLALKDLLDSAKDMQSPDQMGTDLVSNLFV"
     gene            14782..15366
                     /locus_tag="PKK36_04860"
     CDS             14782..15366
                     /locus_tag="PKK36_04860"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNR93925.1"
                     /translation="MSKGRKHISIPVDMTTPVPVWCVGTYDEQDRPNVMTASWAGIVC
                     SRPPCVAVALRDATHSCRGLQARKAFTVSVPSETQYVQADYFGMAGGKTKDKFAASRT
                     TAVRSDKVDAPYVAEFPLILECELKESVVLGLHTLYVGEIMGVKAHPDILNGDTVDAS
                     KLAPFFFSPSERAYYRIGERLSPAFAPGRALMDR"
     gene            complement(15459..16610)
                     /locus_tag="PKK36_04865"
     CDS             complement(15459..16610)
                     /locus_tag="PKK36_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNR93926.1"
                     /translation="MAILLGGLLFLLAVCATAITACIASLNLWQVLFAGAVALAAAAG
                     IFMAVFTRVKHRLLNPYGEMLDAGEWIMRNALGRAAEPDPPGAAPLPPSPVQNKLDAW
                     LDDFKKEYADEVAARTSDMRRDLELAKDFQEAMLERPYPAIPDVHIPGRLRLEFHHCY
                     RPASALGGDFYNLLQAGRDAGGVFIADVMGHGTRSALITSILRTLLVDLLSYARHAPH
                     FLSEINRQFCDLLKSIPNPLFASAFYFVADTTSRIATYSSAGHPSPFHLHRSVGRISR
                     LEVPPPRGAALGVIPDEAFSGGHCRLVPDDVFIFFTDGVYEAFNRQREEFGIERMESV
                     LRRLMYKNVKELVDGLLEEVMLFADGTPLEDDLCVIALEITTKPRVRSA"
     gene            complement(16729..17742)
                     /gene="ruvB"
                     /locus_tag="PKK36_04870"
     CDS             complement(16729..17742)
                     /gene="ruvB"
                     /locus_tag="PKK36_04870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNR93927.1"
                     /translation="MTEHFISQTLEQADTEFDISLRPQRFSDFIGQQKIRERLELFVA
                     AAKGRNDVMDHVLLCGPPGLGKTTLAYILADAMGVNIKSTSGPVIDKPGDLAGLLTNL
                     ERGDILFIDEIHRIQRTVEEYLYSAMEDYVIDIVIDQGPNARSVRLNIPRFTLIGATT
                     RSGLLTAPLRSRFGLTNRLDYYSFEDLQTIILRSARILNADIREDGAMEIARRARGTP
                     RIANNLLRWVRDFAQVRANNRITPEVADQALLMLDVDAHGLDDMDKRILGTILNKFSG
                     GPVGIRSLAVSVGEESDTIEEVYEPYLIQEGYLKRTPQGRVATARARELFGMKPAGQG
                     ELL"
     gene            complement(17786..18373)
                     /gene="ruvA"
                     /locus_tag="PKK36_04875"
     CDS             complement(17786..18373)
                     /gene="ruvA"
                     /locus_tag="PKK36_04875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNR93928.1"
                     /translation="MIAFLEGKLVVKNPTRIVLDVHGVGYEVLIPLSSCDPLPAPGQT
                     CRILTYHHVREDAHILFGFVTEAERELFVRLLAVNGVGPKLALSALSSLSVRDFKRAV
                     LERDVKRLSSISGVGKKTAERLVVELSDKFGEGEAAEALAGETGKPVSRNARDAMMAL
                     IALGYKQMDAAKMVGRVPAEEATTVEEIVRRALTG"
     gene            complement(18370..>18944)
                     /locus_tag="PKK36_04880"
     CDS             complement(18370..>18944)
                     /locus_tag="PKK36_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNR93929.1"
                     /translation="VTEFSVAGDSSGWFAVSRARPGRAQVEIELVVSDSKSGSVLSSF
                     RAKGAASTPRRQADISYKNMTFGGKAFSDSPLGRAAEQALRRGVKGILRGIPAEYWQP
                     RIAEAGTDTVIINGGENARVRKYDEFIVREESREITDPVSGRVIETIAGRQTGRIRVT
                     QVNRASAHARILEGFVRRGQVLEPVRREKK"
BASE COUNT         3648 a         5347 c         6226 g         3723 t
ORIGIN      
        1 aattctcgat tttgggccat tttttactgt ttttggccgt ttttaagcgt ttttgagcgt
       61 tttttggcca tttcttgacg ttttggggaa ctttttggcc gttttttagt tcttctacga
      121 ttttcgtggg aacattggtc gaaatggagc tggcggcggg gaagaaataa agaacaggac
      181 tcagccaggt acaggggctg ggaacataat ttcccggaga atcggcgtga tcacggcggc
      241 ggtgtagtag cgcctctgtg aggcgcattc tgaaacatat gccgctctca gagcggctac
      301 tacaccagaa cccctgcaaa taaaggcgtc cccggctgtc gtgatcatgg aacgacgaac
      361 atgaaaagcc gtctgtgaat tcggtgaaag cggcgtccag ccaccgcact ccaaagcgcc
      421 gtgaaggcgg cgcaatgttt atcatttcgc gccgggagtt gatttggatc tcaaggattt
      481 ccgcgataat tgatgaaggg cctaaaaatg cgattttttg cctgaaaatg gtcaaaaaag
      541 gcccaaaaac gtcaaaaaac gtcaaaaaat ggcaaaaaac cttgattttt ggccaaaaat
      601 ctgcattatt ctgctttttg ggccggttgc tgcggatttt gaccggggat aggcttcgcc
      661 cccgccgatt cccgtctggt tcggctgatt gcagcgctga aaacgggtga tgcatgcatc
      721 acgcagatga ctttaatgaa atgcgccggc gggtgcaacc ggaaaagggg gcgggacgga
      781 aattgtggag gccggtattt ttttgcgcaa aacccttgca ttcgcgcgcg gcggcgatta
      841 gattctggcg gttttgattg agcagggggt gtgctgcgca ggccgccgcc ggactggaaa
      901 gggaagaaga atgaaaaagg atatacatcc gacttatgaa gacgcgacgg tgacgtgcgc
      961 ctgcgggaat gtgattcata cacgttcgac gcgcaaggag atgcatgtga actcgtgctc
     1021 ggcgtgccac ccgtttttca cgggccaggc gaagtacatt gacacggaag gccgtgttga
     1081 gcgtttccgc aagcgctatc agaccgaaaa gaagagctag attttcagaa ggcgggttcg
     1141 gtgtggaggc aatgcccccg agcccgccct ttttatgtcc ggaaacggca tgatcacccg
     1201 cgattatatc cagaagctgc gcgaccgtct tgcggccgtg gagcagaagc tgtccgagcc
     1261 cggcgttacc gcccggcagg aggcgttcca gaagctggtg gcggagcaca agcatttgaa
     1321 gaagacgctg accgccgcgg agcgcttttt ccggctggag gatgaggcgg cttcgacgcg
     1381 ggcgcttttg gaggattccg gcctggattc ggacatgaag cagatggcgg aggatgatct
     1441 cgcgcgcatt gaggaggaga agccgaaggc cgagcgcgac ctgatggtgg ccctgcttcc
     1501 gccccgtccc gaggacagcc gcaacacgat cattgaaatc cgcgccggca caggcgggga
     1561 ggaggcggcg cttttcgcgg gcgacctgtt ccggatgtac acccatttcg cggaggagca
     1621 gggctggaag gttaatttga tggacgccag cccgtcggcg gtcggcgggt acaaggaaat
     1681 tatttttgcg gtggagggcg aggatgtcta ccgggtgctt cgctacgaga gcggtgtgca
     1741 tcgcgtgcag cgggtgcccg agaccgaggc cagcgggcgg attcacacgt cggcggcgac
     1801 ggtggcggtg ctgccggagg cggaggatgt ggatcatatt gacatcaagc ccgaggagat
     1861 ccgcattgat atattccgtt cgtccggtcc gggcggccag agtgtgaaca cgacggattc
     1921 ggcggtgcgc attacgcatc tgccgacggg cctgattgtg cagagccagg acgagcggtc
     1981 gcagcaccgc aaccgcgaga aggcgatgcg cgtgctgaag gcgcgcctgc tggacaagta
     2041 tcagcaggag gaggccgcga agtatgccag ccagcgcaag tcgcaggtgg gctccgggga
     2101 tcggagcgag cggatccgca cgtataattt cccgcagaac cgcctgacgg accaccggat
     2161 caatctcacg gtctacagcc tggaccgcgt catcgagggg gatatcaggg gcctgactga
     2221 cgctctttat gagtttgacg tggcggagcg acttgaaaaa gaactcaaag cctgaaacag
     2281 tccgcgagct gctgcgcgag acggaggatc tgctggcgga gcatggcctg aacgaggcgc
     2341 gccgaatgac ggagctgctg gtttcccatt actgcgggtg ccgtccgctt cagctttaca
     2401 cccggatggg cgatcccgcg gcggcggccc tgcgccgcgc ggtggagcag gggcgcgagc
     2461 ggctgctggc gcatgagccg ctgcagtatg tgaccggcga gacggatttt ttcaactgca
     2521 cgctgaagac ggattcccgc gcgctgattc cgcgtccgga aacggaggag ctggttcagc
     2581 ttgtgctgga cacggcggag ttgtggaaga ttccgctgcc cgcgctcgcg gatgttggca
     2641 cgggctcggg atgcataccg attgcgctgt gcaaagcccg gccgcagggg cgtttcacgg
     2701 gcatagaccg gagcggggag gctctcgcgc ttgcgcggga gaacgcggag cgaaacggcc
     2761 tgggggaact gatccgctgg gtgaggaatg acctgctcgc ggggttcccg cccggatccc
     2821 tggatgcggt gacggccaat ctgccgtata ttccctcggg cgagtgggct gagctgcccc
     2881 cgcatatccg gcggcatgag ccgcaggcgg cgctggccgg cgggccggac gggctggatg
     2941 tgatccgccg gctgattccg caggccgggg cggtcctgaa aaacccgggg tggatttttc
     3001 tggaaatcgg ccattcgcag ggtcacgatg tggcggacat gctgaaacag aatggatttg
     3061 aagaagtggc cgtgaagcgc gatctgtccg ggctggagcg gatggtgacg ggaaggaagg
     3121 catgagcata ctcaaagcaa aaaggaagtt caagattccc cagcaggcga ttctgggccc
     3181 ggtgatcatt gtggtgtttg tggcgctggt gatgcaggcc aactggacgc gccggaatca
     3241 gcggcttttc cggggcatga tgagcggcct cgagcgcctg ccctgccagt actgccgcgg
     3301 caccggaacg atcatggagg aggacgaggc tgaacggcag acggcccttc actgcccggt
     3361 ctgctacggc gtggggttcc gcttcatccg gcggctggat gaaaacgaca agctgtgccc
     3421 cgcgtgcggg ggcatggggc gcctgcagga tcccgatacg gggcattatc gaacgtgcaa
     3481 ccggtgcgac ggccgcggac tgattcaggc gcctcccgga gaccgccggt aatgtttgat
     3541 tccctttccg cctccctcca gaaggtgttc aagaacctgc gcggctacgg caggctttcc
     3601 gaaaagaaca ttcaggaggc gctgcgcgag gtgcggctgg cgctgctgga ggcggatgtc
     3661 aacttccggg tggttaagga tttcatcgcg caggtgcgcg agaaggccct gggccgcgcg
     3721 gtgctggaca gcgtgacgcc gggccagcag gtgatccggg tggtgcatga cgaactggtg
     3781 aatctgctgg ggggcgaacc ggccgggttc actcttgccc cgtctcccgc ggtgatcctg
     3841 atgctgggcc tgcacggggc cggcaagacc accacctgcg gcaagctggc gctgaagtgg
     3901 aagcaggagg ggaagcgtgt gctgctggcc gcgtgcgaca tccgccgtcc ggccgcggtg
     3961 gaccagctgc ggattcttgc ggagcagacc ggcgtggaga ttgtcacccc gcagccgggc
     4021 gagagcgtgc cggggatcgc caaacgcgcg gccgcggaag ccgcgaggcg gaaggccgat
     4081 gtgctgattc tggataccgg cggccgtttt cagattgatg atgagctggt ggaggaggtc
     4141 cgcgaggtgc atcgcgagat tcggcccgcc aacacgattc tggtgctgga cgcggcgacc
     4201 gggcaggagg ccgtgaatgt ggcgcaggcc tttcatgacc gggtggggct caccgggctt
     4261 gtcctgacca agctggacgg cgatgcgcgc gggggcgccg tgctgtccgt gcggcatgtg
     4321 accggatgcc ccgtgatcct ggcgggaacc ggcgagaaaa tggaccggct ggagccgttt
     4381 tatcccgagc gcatggcctc gcgcattctg ggcatgggcg atgtgctttc gctggtggag
     4441 aaggcgcagc agcaggtgga cgccggcgaa atgaaggcga tggaggagcg tctccgcaag
     4501 aacaccttca gcctggatga tttcctgtcg cagatccagc agctcaagaa aatgggaccg
     4561 ctgggggatc ttctggaaat gatgcccggc ggggggcaga ttcccgcgga tgtgaagcag
     4621 cagatggcgg cttcgtcggg ggacgggctg aaggtgaccg aagcgatcat ccgcagcatg
     4681 actccgaagg agcgggagca tccggagatc atcagcgcga gccgccgccg gcggattgcg
     4741 gcgggcagcg gcacgcgggt tcaggatgtg aatgagctgc tgaagcgttt tgaaggggcg
     4801 aagaaaatgg cccggacttt gaaaaaatca caaaaaaggt tgcttcggtt tggaaagtga
     4861 tatatgaaac gggactttct tccccggaat aggtgggaat gaccggctga aagactcagc
     4921 ctggaggatg gagttacatg gcagttaaaa ttagattacg taggatgggt gagaagaacc
     4981 tgcccttttt ccgcgtggtg gtcaccgaca cccgttatcc gcagaccggc cgttttctgg
     5041 agaccattgg ctggtacgat cccaagaaga aagccggatc cgacatacag atggacatgg
     5101 accgcgtgaa ctactgggtg ggccagggcg cgcagatgtc cgtcaccgtg aaggacctgg
     5161 tcaagaaagt atcccggacc acgccgccga aggctccccg gaaagccaag gccgaggctg
     5221 aagctgttga ggcttgagtc ccgatgaagg aactgatcga atacattgtc cgcatccttg
     5281 tggaccaccc cgagcaggtt cagatcaccg aagtggacgg cgaaaagacc gtcatcttcg
     5341 agctgcgctg caacaaggaa gacatcggga agatcatcgg caagagtgga aagacggtcg
     5401 gggccattcg cacgcttctg agcaccgccg ccgccaaaga cggccggcgc gccctgcttg
     5461 agattgttga ctgatgcgcc atgccggcgt gcctcaagat tgacgtcata tccattttcc
     5521 ccgagatcct cgacgcctat ctgggggaga gcatgatgaa gcgcgccgcg caggcggggc
     5581 tggtgtcatt ccgcacgata gacctgcgca accacaccca cgacgttcac cgttccgtgg
     5641 acgaccgtcc ctatggaggc gggcccggca tggtcatgcg gcccgagccg ctgttcgagg
     5701 cggtcgaagc cgtccgcacg cctgagtcaa gggtgatcct gatgtccccg cagggccggc
     5761 ctttcaccca ggcccgcgcc ggggagcttt ccggcgaaaa gcatctgatc cttgtctgcg
     5821 gccattacga gggcgtggac gagcgcgtcc ggggggcgct ggtggacgag gagatatcca
     5881 tcggcgacta tgtgctcacc aacggcatac ttccggcggc ggttgtcatt gacgcgctgg
     5941 tgcgcctgat ccccggtgtg ctgggcgccg gcgaagaggc ggtggagaag gaatcttttc
     6001 aggacgggct gctggagcat ccgcagtaca cgcggccggc ggaattcagg ggcatgaagg
     6061 tgcccgaaat ccttctttcg gggaatcatg aggaaattgc ccgctggcgc agggaccagt
     6121 cgctgcggga gacgcggaaa aaccgcccgg atctgctgtc gtgagggttt gaagtcaaag
     6181 gcttctctct tcgcggggca tttcgctgga aatattctgg aactgacgct tgacgcggga
     6241 acgaatatca gtatgatgcc gccttcattg aggatagaaa aatgagcaag attcttgaaa
     6301 aaattgatgc agagcagcgc aagccggccg atcagatccc tgattttaag atcggggaca
     6361 cggtggatgt gcacgtgcgc attcgtgaag gcgataaaga gcgtattcag gtttattccg
     6421 gtgtggtcat ttcccgcaag ggccacggcg cccgcgaaaa tttcaccgtc cgcaggattt
     6481 cctacggcga gggcgtggag cgcgtgttcc cgattcattc gcccaatctg gccaagatcg
     6541 agctgaaccg ccgcggaaag attcgccgcg ccaagcttta ctatctccgc gagctggccg
     6601 gaaagaaatc ccgcatcaaa gagtcccgcg actgaacagc ctgtgcttgc ctttgagcaa
     6661 caggcatgga cagaaggccg cgtttgcgtg gcgggaattg atgaagccgg acgcggtccc
     6721 tgggccggcc cggtggttgc cgccgcggtt tgtattctcc ccgatttcct ccggcaggag
     6781 gagcatggcg ttttcgaggg gctgaccgac tccaagaaac tttccgccgc ccgccgcgat
     6841 catttttttg aagtgctgac cacgtgcggc gcggtccgtt gcgggatcgg ccgcgcggag
     6901 gcggacgaga ttgattccat taacattctt caggccacgc atctcgcgat gcggcgcgcc
     6961 tttgatgcgc tgcggccggc ccccgatttc gcgctggtgg acggcaatcg tcttccgccg
     7021 cttccctgtg ccgcgctcgc cgtgaccggc ggcgacggaa agagtctgtc catcgccgcg
     7081 gccagcatcc tggcgaaggt gacccgcgac cggctgctgc aggaggcgga cgagcggtat
     7141 ccgggatacg gttttgcgcg gcacaagggg tatggaacgg ccgggcattc cgcggcgctg
     7201 cgaaagctgg ggccttcgcc ctttcaccgc atgaccttcc gtcccgtcag ggaatgcctg
     7261 gagaggaacg cccggtgatc gcctggctgc agagactgct gcgcaggaag aagtctcttc
     7321 cggaacacct gaaacggggt ttgtggggag agaaggtcgc ggaagatgcc ctgaagaaaa
     7381 aaggttatgc gattctgggc cgccgtgtgc ggatggggaa gcggggggag cttgaccttg
     7441 tcgcgaagca gggcgagacg ctggtgtttg tcgaggtgaa aacccgcgcc ggagagcagt
     7501 tcggccggcc cttcggcgca attaaaagcc acaagaagaa gatgctgatg aacgcggcga
     7561 agtgctatct gctcaaactg aaggaacggc cgcgctattt ccggttcgat gcggtggagg
     7621 tgatcgggcg ggaaggcgcg ggggaaccgg tggtgcggca tatcgagagc gccatcaaaa
     7681 tcacaaggaa atatcgcatt cagtggtgag caagatgcgg atgaaaaaga cgataacaat
     7741 tgtttttctt ctggcggcgt ccgcggcgtg cagcggaatt gaagatttgg attttacgga
     7801 aatggcgacc gccggactgg aatggtttga ggagaatgtt cctgaatcgc tgcatgcgcg
     7861 cataggagaa gtggactggg agtattacac ggagaagctt ctgggacatc tgcaggagta
     7921 ttcctgggag gacatggccg gatgcgaaga gcaggcgcag acgctgctgg cctttctgaa
     7981 ccagtatgag cgcctggaac cgctggcggt gtggctggaa acgcgcatgg attatttcgc
     8041 gatggctgat gagttcctgc agcagctgag agaacttccg cccgagccgg aagtccggcc
     8101 ccggcccgtg ccgggccctg cgcctcttcc gccgcctgcg ccgcgcaggc ggcctgtgta
     8161 tccgccgcag aagaaatggg aagccaagct gaagacccgg cccaaaccgg cccgtgccga
     8221 tggcatggtg ccggagctga aaaccatatt tgagcgcgaa ggagttccgg gcgagtgggt
     8281 ttggattgca gaggttgaat cctcgttcaa tccctcggcg cgcagcccgg cgggcgcggc
     8341 gggccttttc cagttcatgc cggcgacggc gcgccgcttc gggctgaaga cctccccgca
     8401 ggacgaacgc ctggatccgc ggaaaagcgc cgcggccgcc gcccggtatc tgggcgtgct
     8461 gcaccggcgg tttgattcgt ggccgctggc cctggccgcc tataatgcgg gggagggacg
     8521 cgtcggcggg ctgctcaaga aacacggggc cggagatttc gacggcatag cggagaaact
     8581 tccgctggaa acccgccttt atgtgccgaa ggtgctggcg accgtttccc tccgcgaagg
     8641 cgtcaatgcg gagcacctgc ccccgccgtc ctgagccgcg tgttacgcat tttacaaacg
     8701 gtcccgaatc tggttttcta ttcctccatg aggtttcgcc gtgccatcct gatcctgccg
     8761 gtcgccgttc tgggcgtcct gatggtttcg tcgcgcgcag agccggaagg ggcccgtccg
     8821 aaagtcggcc tggtgctgag cggcggcggc gccaaggggt tcgcgcacat cggtgtgctg
     8881 aaacagcttg aggagatgca tattccgatc gactgcattg ccggaaccag catgggcgcc
     8941 atcatgggcg gcctgtatgc ctccggcatg tctccgcgcg aaatggagca gatttttacg
     9001 ggccttgact ggtgggacat cctgaaggac cagacacagc gcaaggatct cgcgttccgc
     9061 cggaagaagg acgaccagcg ctatctcggg aatatcgaac tgggcttcaa gcacgggagg
     9121 tttcgtcttc cgcgcgcgat ggccgccggg cagaagctga acaatctgct ggtcacgctg
     9181 acgcgggatg cgcgcaggaa ttttgatgac ctgaacattc cgttccgcgc ggtggccacg
     9241 gatctttcca gcggcgaccg gattgtgatg ggagatggaa gccttgcccg cgcgatgcgg
     9301 gccagcatgg ccattccggg cgtgttttcg ccggtcgaga ttgacggcat gacccttgtg
     9361 gacgggggca ttgccgacaa tattcccgtt gatctcgcgc gtgaaatggg cgccgacatc
     9421 atcattgcgg tggatctggg cgccatggac atcgcctcca cccgccggtc gcagggcgac
     9481 attgcagagc tgctcgcgca cacctatcag ctcattcgcc ggccgcagca ggaggagcag
     9541 atgcgcgacg ccgatatcgc ggtgacgccg gacacctcca tgttttcctc ttccgatttt
     9601 cacaaggccc gcacgatcat cgggacaggc cgccgcgccg cgcgcgcggt cgaggagcag
     9661 ctccggccgc tgagcatcag cgaggaggag tacgcggagt ggctgcgccg tcatcgccgg
     9721 gcggcagacg atacgcccgt gatccgctcc attgccatca ccggagaaaa gcatgtggac
     9781 gagcggatca ttgcccgcca gatcaagacg cgcctgaacc gccccctgga ttatgaactg
     9841 cttgaagacg acctttcccg cattcacggg ctcggatatt tcgaatcggc catgtacggg
     9901 atttcgggcg aaggcgggca gagagacatg accatcgaag tgtcggagaa gccgtggggg
     9961 cccgggtatg tccgtttcgg cctgaaactg gatgcggact ccgaggaaaa cagctcctgg
    10021 tcggtgctgg cgaattacac gcgccggccg ctgaaccgtc tgggcggcga attcctgctg
    10081 gacctgcagg cgggcgagaa gcgccgcatc atggcggagc tttaccagcc gctcgatttc
    10141 cgcggagcct tttttctttc tccgtcggcg gagtattcct cctcgatgat ggatgtctat
    10201 gacggggata ggaaggcggc ggaatatgac gtggaggaat tctacggcgc gctgaatgcc
    10261 ggcgttcaga tcgggaaata cggtcagatc cagctcggca tggtggccgg ccgcgcccgc
    10321 gccacggtgg atgtgggcga ccccgatttg gaaaacattg atcaggatgc cggcggctgg
    10381 acgggacaca tcgtgctgga ccgccgcgac agcgatgtct ttgcgcgaga gggcttcctg
    10441 gttgtgctgc agggtttttc gggcgacgag atgctgggcc atgaggattc gtacgagaag
    10501 gtgagctggc tttcgcagca ctacatgacg ctcggccggc acacgatgtt tgcgcatctc
    10561 gaactgggca ccagtctgga aagcgagctg ccgctgtatg acgagtttgc gctcggcggc
    10621 ctgtattcga tgtccggcta tgcggacggc cagctgcggg gacagtatct gggcgtcggg
    10681 cggctgggat acatgttccg catcagccag cttccgccag acttgggacg gggcgtttat
    10741 atcggcgcct ggggcgaggc cggcaatgtc tggcaggacg aaggcgacat tgacagcgac
    10801 agcctgctgt acggggcgac ggtcggcctg ggcgcggaca cgtttctggg acccctctac
    10861 tttgcctatg gccggtctga acacggccgc gaccggtttt acttttcact gggaaacaac
    10921 ttttgaccgg gcttgagttt ccccgtctgc cggtttagca tgtgcccctg ttattcggag
    10981 taaatatgaa agcatggaca cttgcaggat taatggcgct ggttctggcg gtcggagcga
    11041 tcgcgcagga gcagccggac aaccccgaac cggaggagaa catcatggtc gtgatcaaga
    11101 caagcaaggg cgacatcaag gtcgagctga acgagaagaa ggcgcctggg acggtgaaga
    11161 atttcctggc ctatgtggat gccggtcact atgacgggac catcttccac cgcgtgattg
    11221 acggcttcat gattcagggc ggggggttca caaaggacat gcgccagaaa ccgacgcgcg
    11281 cgcccattga aaacgaggcc gccaacgggc tcggcaacga gcgcggcacc atcgccatgg
    11341 cccgcacatc cgatatccac agcgcaacca gccagttttt catcaatgtc gtgaacaacg
    11401 cattcctgaa tcaccgcgac aaatccgcgc aggggtacgg ctactgcgta ttcggccggg
    11461 ttgccgaggg catggatgtg gtggatgcga tcaagtccgt caggacggcc gcgcaggacg
    11521 tacccgtgga aacggttgag atcatcagca tcacgcgggc cgaatagcgg cttgcctgat
    11581 tatttcacgg tgctgcgggt ggtcgcggcc agcgccttcc agagtttctc gggcgtgtcg
    11641 gcatccagca gcttgtcgcg gttttccttt ttctggaagc tttccgtgag gccggccagc
    11701 agcttcaggt aaaaagcgct cgcgccggtt gggatcagca ggaaaaagat gatgcgggtc
    11761 aggtttttcc ctccctgctc gcaccggatg ccttttttgg aagtcgcgag ggcaagggtc
    11821 agtccgccgc cctcgatgcc gcggacgtgc gggaaggcga tgccgtgctc gaccgccgtg
    11881 ctgatgatgg actcgcgggc gatggcggcg tcgcggagtt tgcggccgtt gtccacaaac
    11941 tgttcggcct ccagcttcat gctcatttcc tcgatcgctt catcccgggt ggcggcctcg
    12001 atatggggca tcatcagatt cagcgcggag aaccgcgcga tgcctatttt gggcgcctca
    12061 aagcgctggg ttctccggga gatgcgctgg ggaagggtct cgggcggcat gtacaggatg
    12121 cgcgcgcagg tcgggcaggt ggatacctgc gttccggcgg ccaccagctg gatcaggctg
    12181 ctgggcagct gcatggcgca ggcggaacag cgtcctccgg cgatcggaac aatggccacg
    12241 tggcctttct gctgcagctt ggtgaatttg ctgctcaggt cgggcgggag ttttttgcgg
    12301 agcgcctcaa tggacgcgtc gagttcagtg agctgaggat tggagacaac cgcctcctgc
    12361 tgggcgcggg cctccgccag ttcctggagt tgaaccagaa gattaataac gggtttcatt
    12421 ttacctttct gccgtgaatg cgatttgctg atcgtcatgc aagcggcttc ataccgggac
    12481 tttttctacc agcccctgcc ccgggatgca atggatatcg aaaaaaaaat gaaggcgggt
    12541 ttcagtttct gcggcaaacc gcgaatgcgg tattgaccgc tccgcccgtt tggaacatga
    12601 tgtacggtct ttttgattga gaggcccagt tatggatgtg aaaaaagcca tttccaccga
    12661 tacggttttt caggatctga aaagcacgac aaagaccggt gtgattgaag aaattcttga
    12721 tctgctgatt gccgcgggca agatccggaa agaaaaccgt gccgaggtgc tgaatgccct
    12781 tcttgtgcgc gagaacaaga tgtccaccgg catgcagcac ggcatcgcca ttcctcatgc
    12841 caagaccgat gcagtcgagg atctggtggc cgctctggcc atcaagaaag agggtattga
    12901 ttttgacgcc ctcgacggga agcccagcca tattttcatc atgaccatat cgcccgtgaa
    12961 gcgcaccggg ccccatattc agttcctcgc cgagatcagc cgcaagctga accagccgtc
    13021 gatccgggaa aaactgctcc tgacccagac gaaggacgaa atccttcagc ttctgtgagg
    13081 cgcatggaaa cgatccttgc cattcccggt ctgcttctcg cctcggtggc cgagcatgcc
    13141 gttgaacgcg acatgaccca tctcatgacg cagtacattc tgcagctcgc tgtcatcatg
    13201 ctggccgccc gcatgggcgg ttttctggtg aagcgctacc tgaacgggcc agcggccctt
    13261 ggggaactgg ccgcgggcat ggcgatcggg ccctgtgcgc tcggcgcatg gcacattccg
    13321 ggatgggggg cgctgtttcc tcccgtggcg ggcatattcc cggtgtccac ggagctttat
    13381 gccctggcca ccctggcttc gattgtcctc ctgtttcttt ccggactgga aacggacctg
    13441 cgtgtgtttc tgcactattc ggttgcggga atcgtggtcg gaacgggcgg gctgctggct
    13501 tcgttcttcc ttggcgcctg gtgcgcgctg ctgttcaacg tggccgattc cctgatggat
    13561 ccgtccgcgc ttttcatggg agcaatggcc accgccacct cggtgagtct tcccgcgcga
    13621 atcctttccg agaaaagaaa aatggcttcc cccgaagggg tcaccatact ggcggccgcc
    13681 gtgtttgacg atgtgctcgg catcattgcg ctggcgatca ttgtcggcat gagcaagacc
    13741 atggccagcg gcgccgtcat ggactgggga aagatttccg tcgtggcggt caagagcatc
    13801 ggcttctggc ttattctcat ggcggcgggg ctcctgctgg ccaagcatat tgcgcgcgtt
    13861 ttgaaactgc tgcgctcccc gaacaccatc gcggccatct gctttggcct cgctctgctg
    13921 ctggccggta tttcggaaat ggccggtctg gcaatgatca ttggcgccta tatcatgggt
    13981 ctttcgcttt ccggaacgga cctggtctat gaactgcagg agcagcttca aggcgtctat
    14041 gacttcatca ttcccgtctt tttctgcgtc atgggcatgc tggtgaattt cgccgcgatg
    14101 aaggggctga tattcttcgg gctggtctac acggtgatgt gcattatctc aaaggtggcc
    14161 ggctgcggaa ttcccgcgct gttcatgaag ttcacccccc ggggcgcctt ccgcgtcggc
    14221 gcgggcatgg tgccccgcgc cgaggtcacc ctgattgtcg ccggtatcgg cctttccgcc
    14281 ggctatatcg ggccgaacct tttcggcgtg gccgtcatga tggccatgct caccaccgtt
    14341 gcggcgccgc ccatgatcct cagcgcattc cgcggcggct ccggcgtgcg cggtgaagaa
    14401 accgcgcagg agcaggagct ttccaccgcc gtgctcacgt ttccctccgc cgacatggcc
    14461 gagttcatct gcgcccgcat cacgagggct ttccgcaacg aggaattttt tgtccacatg
    14521 ctcgacagcg atgtgccgac ctatcagatc cgcaaagagg acatggcttt ttcgattgtg
    14581 caggatgatg tccacatcac catcagcatg ccgcttcagt atgagcatgt cggccggctt
    14641 atcgtgctgg aggaggtgct ggccctgaag gatcttctgg attcggccaa agacatgcag
    14701 agtccggatc agatgggaac cgatctggtt tcaaatcttt tcgtataaat accagcggct
    14761 ggccgcgcgg aggaaacagc catgagcaaa ggaagaaagc acatatccat acccgtggac
    14821 atgaccaccc cggtcccggt ctggtgtgtc ggaacctatg acgagcagga ccgtcccaat
    14881 gtgatgaccg ccagctgggc gggcattgtc tgcagccgtc cgccgtgcgt ggccgtggcg
    14941 ctgcgtgatg ccacgcattc ctgccgcgga cttcaggccc ggaaggcctt caccgtcagc
    15001 gtcccgtctg aaacccagta tgtgcaggcc gactacttcg ggatggcggg cgggaaaacc
    15061 aaggacaagt ttgccgccag ccgaaccacc gcggtccggt ccgacaaggt ggatgcgccc
    15121 tatgtcgccg aatttccgct catactggaa tgcgaactga aggaatccgt ggtgctgggc
    15181 cttcacacgc tgtacgttgg cgagatcatg ggcgtgaagg cgcatccgga catcctgaac
    15241 ggcgacacag tggatgcctc gaagctggcg cccttctttt tttcgccgtc cgagcgggcc
    15301 tattacagaa taggcgaacg cctgtcgccc gcctttgcgc cgggccgcgc gctcatggac
    15361 cggtgagtcc gaaaaagttc caatgactgg aaaatcccgc cgaaaaagtt ccaatcattg
    15421 gaactttttt cccgaaaact tccagtcatt ggaacttttc aggcactgcg cacgcggggc
    15481 tttgtggtga tttcaagcgc gatcacgcac aggtcgtctt ccaggggcgt gccgtccgcg
    15541 aagagcatga cctcctccag caggccgtcc accagctcct tgacgttttt gtacatcagc
    15601 cggcggagca cggactccat gcgctcgatg ccgaattcct cgcgctggcg gttgaaggcc
    15661 tcgtagacgc cgtcggtgaa gaagatgaac acatcatccg gcaccagccg gcagtgtccg
    15721 ccggagaacg cctcgtccgg aatgacgccg agcgcggcgc cgcgcggcgg cggcacttca
    15781 agccgcgaga tgcggcccac gctccggtgc aggtggaagg gactgggatg tccggcggaa
    15841 gaataggtgg cgatgcggga agtggtgtcg gccacaaaat agaaggccga ggcaaacagc
    15901 ggattgggga tgcttttgag cagatcgcag aactggcggt tgatttcaga aaggaaatgc
    15961 ggggcatgcc gcgcatagga cagcaggtcc accagcaggg tgcgcaggat ggatgtgatc
    16021 agcgcgctgc gcgttccatg gcccatcacg tcggcaatga acacgccgcc ggcgtcgcgg
    16081 ccggcctgga gcagattgta gaagtcgccg ccgagcgccg acgccgggcg gtagcagtga
    16141 tggaactcca gccgcaggcg tccgggaatg tgcacgtcgg gaatggcggg atagggccgc
    16201 tccagcatgg cctcctgaaa atccttggcc agttccaggt cgcgccgcat gtcggaggtg
    16261 cgggcggcca cttcgtcggc atattccttc ttgaaatcat ccagccaggc atccagctta
    16321 ttctgaacgg gcgaaggcgg caggggtgcg gcgcccggcg ggtcgggttc cgccgcgcgg
    16381 cccagcgcgt tgcgcatgat ccactcgccg gcgtccagca tttcgccgta ggggttcagc
    16441 agccggtgtt tcacgcgggt gaacaccgcc atgaaaattc ccgcggcagc ggccagcgcc
    16501 acggctccgg caaacaggac ctgccaaagg ttcagggagg cgatgcaggc cgttatggcg
    16561 gttgcgcaaa cggccagcag gaaaagcagt cctcccagca gaatggccat ccatttgtgc
    16621 aatcttgtct gcggcttcat cttggcgcgt cagcaatccg tccctgttaa caatagcact
    16681 gtaaactatt ggtttgtgga atcaaaggca aggcttcttt aaaccgaatc agagaagctc
    16741 tccctgcccg gcaggtttca tcccaaaaag ttcacgggcg cgggcggtgg ccacgcggcc
    16801 ctgcggggtc cgcttgagat agccctcctg aatgagatac ggttcgtaca cctcttcgat
    16861 ggtgtctgat tcttcgccga cggaaaccgc cagcgaccgg attccgaccg ggccgccgga
    16921 gaatttattc aggatggttc cgagaatccg cttgtccatg tcgtccagcc cgtgcgcatc
    16981 cacgtccagc atcaggaggg cctggtcagc cacttccggc gtgatgcggt tgttggccct
    17041 gacctgcgcg aagtcgcgca cccaccgcag caggttgttg gcaatgcgcg gcgtgccgcg
    17101 cgcccggcgg gcgatttcca tggctccgtc ctcccggatg tccgcgttga gaattcgcgc
    17161 cgagcgcagg ataatggtct ggaggtcttc gaagctgtag tagtccagcc ggttggtgag
    17221 cccgaaacgg gagcggagcg gggccgtgag cagcccgctg cgggtggtgg ccccgatcag
    17281 cgtgaagcgc ggaatgttca gccggaccga acgggcgttc gggccctggt caatcacgat
    17341 atcaatgaca tagtcttcca tggccgaata gaggtactcc tcgaccgtcc gctggatgcg
    17401 atggatttca tcaatgaaga gaatgtcgcc gcgctcaaga ttggtgagaa gcccggccag
    17461 gtcgcccggc ttgtcaatca ccggcccgga cgtgcttttg atgttcacgc ccatcgcatc
    17521 ggccaggatg taggccagcg tcgttttgcc cagccccggc ggtccgcaga gcagcacatg
    17581 gtccatgaca tcgttgcggc cctttgcggc cgccacaaac agctccagcc gctcgcggat
    17641 tttctgctgg ccgatgaaat cagaaaagcg ctgcgggcgc agcgaaatgt cgaactccgt
    17701 atccgcctgc tcgagggttt gcgatatgaa atgttcagtc attgcgaagc agtataggaa
    17761 atgcgccgca gggagtaaag ccgcatcagc cggtcagcgc ccggcgcaca atctcctcga
    17821 cggtggtcgc ctcttcggcc ggcacgcggc caaccatttt ggccgcgtcc atttgtttgt
    17881 agccgagcgc gatcagcgcc atcatcgcgt cgcgcgcgtt ccggctgacg ggttttcccg
    17941 tttctccggc aagcgcctcg gcggcttcgc cctcgccgaa tttgtcggag agttccacca
    18001 ccagccgctc ggcggtcttt ttcccgaccc ccgagatgga gctgagccgt ttcacgtcgc
    18061 gctccagcac cgcgcgcttg aagtcgcgca cgctcaggct gctgagtgcg ctcagggcca
    18121 gcttgggccc gactccgttg acggccagca gccgcacaaa cagttcgcgt tcggcctccg
    18181 tcacgaagcc gaaaagtatg tgggcgtctt cgcgaacgtg atgataggtc aggattctgc
    18241 aggtctggcc gggcgcgggc agggggtcac agctgctgag gggaatcagc acctcgtagc
    18301 cgacgccgtg cacatccagc acaatgcggg tgggattttt aacgaccagt tttccttcca
    18361 gaaaggcaat cattttttct ccctccgcac gggttcaaga acctgtccgc gccggacaaa
    18421 accttccaga atccgcgcgt gagcagaggc cctgttcacc tgtgtcacgc ggatgcgccc
    18481 cgtttggcgt cccgcgatgg tctcgatgac gcgtccgctg accggatcag tgatctcgcg
    18541 gctttcctcg cggacgatga actcatcata tttcctgacc cgcgcgtttt cgcctccgtt
    18601 gatgatgacg gtgtctgttc cggcctcggc gatgcgcggc tgccagtatt ctgccgggat
    18661 accccggaga attcccttca cgccccggcg gagggcctgt tccgcagccc tgcccagcgg
    18721 ggaatctgaa aaggccttcc cgccgaaagt catgttcttg tagctgatgt ctgcctggcg
    18781 ccggggggtc gaggcggcgc ctttggcccg gaaagacgac aggaccgagc cggactttga
    18841 atccgacaca accagttcaa tttccacttg tgcccggcct ggccgtgccc gcgagacggc
    18901 aaaccagccg cttgagtctc cggcaacgga aaattcagtc acgg
//
LOCUS       DAOQWR010000040        18328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3430_length_18328_cov_5.891917, whole genome shotgun sequence.
ACCESSION   DAOQWR010000040 DAOQWR010000000
VERSION     DAOQWR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_18328_cov_5.891917"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..2167
                     /gene="glgB"
                     /locus_tag="PKK36_04885"
     CDS             41..2167
                     /gene="glgB"
                     /locus_tag="PKK36_04885"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNR93930.1"
                     /translation="MGMHPFGGPPPGVVVRSFQPYARAVSVIPGTGGDPRPMTRVHND
                     GFFELTLPGAQMFDYRLRMTGFDGHTWELDDPYRFPPQITDFDLYLFGEGTHFRTYKK
                     MGAHPLTINGVEGVHFAVWAPNARRVSVIGVFNRWDGRHTPMQNRGSSGLWEVFVPAL
                     KPGDIYKYEILGPNDYLTERADPYAFACELRPKTASVIWDIEKYPWGDGDWMSRRRDT
                     RWHDKPISVYEVHLGSWRRNGAHGEYWLSYRQLADTLIPYIKDSGYTHIELLPVSEHP
                     FDGSWGYQTIGYYAPTSRFGTPDDLKHFIDRCHQEGIGVILDWVPAHFPKDGHGLGYF
                     DGTHLYEHSDPRKGEHMDWGTLIFNYGRNEVRTFLLANAMFWADVYHIDGIRVDAVAS
                     MLYLDYSRKAGEWIPNQFGGRENLEAVSFLKKFNELIHGEYPGFITFAEESTAWPMVS
                     RPTYLGGLGFDFKWNMGWMNDTLEYIQKEPVHRKYHHGSLTFSLIYAFTENFILPFSH
                     DEVVHGKRSMLDKMPGDFWQKFANLRVLYAYMYAHPGKKLLFMGCEFGQWLEWNCNQS
                     LDWHLLQFEPHQKLLDCVRDLNRIYREEKSMHEVDFTWEGFQWIDLHDSEQSIISFLR
                     RARDGSDFVVCLFNLTPVPRWGYRVGVPKPGLYREIFNSDSDLYGGSNLGNAGEVPSD
                     PIPWQGQPHSLLLTLPPLGALYLKPS"
     gene            complement(2195..2980)
                     /locus_tag="PKK36_04890"
     CDS             complement(2195..2980)
                     /locus_tag="PKK36_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93931.1"
                     /translation="MNIRFAGVVLVAAALTGSAAEGGEADLSIPLEDAKGFYADKIQG
                     HLELGLHFSAVRLLDSDKWDDAAGEGFVGTIDHFDEVQTLLPYNLSVRYFLNEHWGVA
                     CQWEQMEAEAVTHDQGDVDGVFTSSGPSLLLVGRMPLSRGLVPYAEAGAHFPTVDFEM
                     EDWWHMGYPTSGTYARGNKQPYHGHTRYMDTEEEDAVCLAAGLGLLWKCREHWAVDLN
                     MRYLDVNAVSHHYSRDGQRINDHGNSSIPLSHLAFGAGVAYQF"
     gene            complement(3069..3154)
                     /locus_tag="PKK36_04895"
     tRNA            complement(3069..3154)
                     /locus_tag="PKK36_04895"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3118..3120),aa:Tyr,seq:gta)
     gene            3265..4125
                     /locus_tag="PKK36_04900"
     CDS             3265..4125
                     /locus_tag="PKK36_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR93932.1"
                     /translation="MRIIHFSDLHVWRPGLETGDLWYPKRMLGYVNLALRRKFKYPPG
                     LGRAVVDEIARSDADAVVFTGDMTTASLLGEFEDAADLFSPLLEKWEDRFVVLPGNHD
                     RYTPYTISRKFYECLFADRGPVTRTDVGGHWSIVSFDASRPYMVRSNGMISLALAAHL
                     AQILREVADAGRTCVLAGHFPYDHPRGCREGLQHRLLGARRLIEVINEFKPPVYLHGH
                     KHVRWVFQSPKTPDTVCINSGSAGLKSEDPLKRAGFVTIDLDDKGALQGVLACSARRD
                     EEKFETVALC"
     gene            complement(4122..5621)
                     /locus_tag="PKK36_04905"
     CDS             complement(4122..5621)
                     /locus_tag="PKK36_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR93933.1"
                     /translation="MSTEPRKAWRCTLCGYIHQGPVPPNYCPLCGAPDTDFEPYEAPA
                     AAEPQPDEVKQWRCIVCGYIHEGPEPPDFCPLCGASKDEFEAVKQPKAPQSPTEKSIH
                     VAIIGAGIAGVSAAEAVRDASPKSSITLICGENALPYYRLNLTRYLAGEIDRATLPIH
                     PKSWYEENRVELLSGERVDHITPREHFFTLANGRQIFYDKLILAMGSHPFIPPVEGTD
                     MDGVFALRTVRDAEDIIARTAGKPRCVVIGGGILGLECAGALAQRGLQVAVLENHEWL
                     MPRQLNRRAGEMLENHLRKLGIALEKNAQTRAVTGRGKAEAVELQDGRSVPAELVLLA
                     TGVRPDTYLARRAGLEVNRGIVVNNHLQTSDPDIYAAGDVAEHHGVLYGIWGPSQYQG
                     RMAGMNAAGMDTLFGGLPRSNTLKVLGIHLASVGKVQPEDGSYTGVEGTSGDDYAYFL
                     FRDGKMAGGILLGSTSCAAGLKNAVESERDFSELLKRPPSVRTVMAALA"
     gene            complement(5624..6124)
                     /locus_tag="PKK36_04910"
     CDS             complement(5624..6124)
                     /locus_tag="PKK36_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNR93934.1"
                     /translation="MATIDNLKDSFAGESQANRKYLAFAKKAEADGFAQVAKLFRAAA
                     EAETVHAHAHLRAMGGIKDTAANLEAAIEGEGFEFQKMYPPYLQEAIAEGSRQAEMSF
                     KYALAVEEIHYNLYRAALDSVKAGNDLPARRIYVCPVCGNTEYDEAPDKCPICGVPAS
                     KFIEIA"
     gene            6258..6740
                     /locus_tag="PKK36_04915"
     CDS             6258..6740
                     /locus_tag="PKK36_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNR93935.1"
                     /translation="MRRFCMWLALILCMHGGFSSAAADSVRVPRKTPDREITMRVTGY
                     CPCRECCNWKRNWLFRPVIASGPQKGRPKKVGITASGKRAGPGTIAADARLFPFGTIM
                     YVPGYGYGIVEDRGGAIRGHHIDLFYRSHATAKKWGSKKLRVQVWYPPKTSRKPSRKK
                     "
     gene            complement(6743..7729)
                     /locus_tag="PKK36_04920"
     CDS             complement(6743..7729)
                     /locus_tag="PKK36_04920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNR93936.1"
                     /translation="MPDQNQSALPDFSHLTPDTVLHLVETALGARCTNMCRPLTSYIN
                     RVYDVELDNGSWVVVKFYRPNRWSREALLDEQDLLEELRAQEIPVIAPMRGADGEALQ
                     EYHGTYFAIFPKRGGRPLDEPNEAQWRELGRLLARVHSVGAAHPPRDRIEIGPRQSAA
                     MHFQFILDADAVPRQLRKNYEEAARGILDLIEPLFDGVEKIRIHGDMHRLNILQRPDE
                     PLALIDFDDMALGPPVQDIWMLLPGHLKHSTPDLNLLLEGYETFRDFDRQTLRLIEPL
                     RAMRFLHFTAWCARQKADGGFARLAPDWGAESFWRREIHDLNQQRQEILDAL"
     gene            complement(7783..11379)
                     /locus_tag="PKK36_04925"
     CDS             complement(7783..11379)
                     /locus_tag="PKK36_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNR93937.1"
                     /translation="MNQSSYILCRKLAVFSAAALMAAATRAQSTNFPGELGSLVRSNG
                     VTFRVWAPNASTVAVRGDFNGWGTTAMTSEGSTGYWSVDIPSARPGQEYQYFLNGSIW
                     RRDPRARRVTHSTGNSIIYDPDAFDWGDTPIPMPDRKDAVLYQMHPGTFNAEDWIPSS
                     FDKNIERLDHLRNLGINVIQLMPVNEFATDRSWGYNPSDLFAIESALGGPDALKRFVK
                     AAHERGIAVFTDVVHNHYGPTDLALWQFDGWSQNGLGGIYFYNSTDGKAYTWWGSTRP
                     DYSRTEVRDFIRDQIMMFVEEYRVGGFRWDSVFNIIYYNDGYNHNADGEYLLRDINWE
                     LSQNHPNVFRISEDNAFDYSMNFESMWDVQFHDHIEWQVTQGSDANRNMSALRDHLLA
                     NANGSRVIFSEAHDYVGDLNDAVRLPRAIDGADPAGIWARKRQLLAAAAVMTKPGIPM
                     IFQGQEMNEDWTFSSSTSLRWALTNTHAGIVRAYRDLIRSRRNLRGGMQGLKGTGITV
                     HHVDDTRKVIAYVRWDAGGGADDAVVAANFSANTFTNGSYLIEFPSEGVWYSHFNSDS
                     TEYGADFGNIGFTQVTASGSPAMAAVDMGMYSLQIFSKTPPTGAGAVTFDPPAPEGCV
                     DVTISYDPAGGPLAGATNVVLYQGRNNWLDIQETVMTNRGDNTWVVTASIPSQTHELN
                     LIFHDGAAEESRVWDNNESMNWRVNVSGCASLPALVTVSPAVPAGCVPVTITYEARES
                     VLSTAEAVFVHFGRNGWQDVQTLAMTNPAAGAWSVTADIPDSTYQLDFVFHDGAEGTN
                     RVWDNNDSRDWTVFVSGCYNTQSPGIAITNPAADLHVAATQAVINIEGSSYGLAGDLI
                     WTNSHAGSGGSIPSAANWLVSGIPLLTGVNLFHISGLRDTVNLNAGANDSATNVVYDA
                     GWPDGANGGAAWGGGWNLTATPNSGHFMATNTVYLDSGSRAWGMWANSGGFCEAVRPF
                     AARLNPGDVFTIRLENGGVDDPDSSIGVALENHFGQSLFEFLFVAGGTNYLINDELTT
                     RNTGIPWSNSGLTLEFELTSGSTYRLTAGPDEFEGNLGISSEMLIRQVRIWNASAGGG
                     PERDLFVLDMSIDGDPLEPIPSAAEVAVTRAPGDEPVAPPDVSGVAAGQTALVVSNSV
                     AGQLYDIYVSTNLTGGWMPLGLNVPGGGGDITLFFTNTADMVFYTIGTRKIP"
     gene            11577..11840
                     /locus_tag="PKK36_04930"
     CDS             11577..11840
                     /locus_tag="PKK36_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93938.1"
                     /translation="MNLLLYMFYPLVGLIEWYLALRRTLACVRRERAVLVTIVFIENL
                     LGLAVLQNFITSGNWLIAIFYSMGAGLGALLSLRIASGDTSKT"
     gene            12206..15046
                     /locus_tag="PKK36_04935"
     CDS             12206..15046
                     /locus_tag="PKK36_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR93939.1"
                     /translation="MAVWRQGGYPGVTRTTARLLEYWTNPQREKKLFFCQIEALETLI
                     YITEAAKKYGDTWIENELKSANEGANPGLFRLALKMATGSGKTVVMGMMIAWHALNKI
                     ANAQDNRFSDTFLIVTPGITIRDRLRVLLPNDPGNYYRQRDLLPAYMMDELGQAKIVI
                     TNFHAFKQREKIAAGKLTKSILSTGDNGAFTETPAQMVRRVCRELGSKKQIVVINDES
                     HHCYRHKPDGEEVVLKGDERKEAEAREEYARVWISGLEAVRDQIGLKVVYDLSATPFF
                     LRGSGYSEGTLFPWVVSDFSLIDAIESGIVKVPRVPVADDSMKGDQPTYRDIWTRIRE
                     GLPKKGRRTDAVTGEPKLPKELQGALHSLYDNYRKYYELWEKNPDARANNQTPPVFIV
                     VCNNTNVSKLVFDYVAGWEKTISKEKTALVPGAFPVFSNVVDGQWSVRPNTILIDSEQ
                     LESGEAMSADFKSAAAREIEEFKAEYRQRFPGRDTEKLTDEDLLREVMNTVGKPGKLG
                     EHVKCVISVSMLTEGWDANTVTHILGVRAFGTQLLCEQVVGRGLRRRSYVTENRTIDV
                     GGKPVEINAFPTEYAEVYGVPFSFIPCSGSTGPGPTPPVPTTRVRAMDERKNCEITFP
                     RLTGYRYELPGEILIAKFTDDSKITLSTEEVPWKTINAPIVGESSVHTLEDLKSRRLQ
                     QVAFLLAKLTLEKYFRADSGGITNGGTLSVASEVPTERNPPTDVKAWLFPQLLEISRS
                     WLDTCVKCKDNTFPQLLLLLEYAHDAADRIYRSIVQGTEGEKTLLPILQPYDTIGSTK
                     FVDFDTTRPVWSTRADKCHVSHVAADTNSWEQKLAQTLEDMDEVICYVKNEHVGFTIP
                     YTLNGEEHSYIPDFIARMDDGHGADDPLNLILEVTGEKKKDKAAKVATAKSLWVPSVN
                     NQGEFGRWGFVEITDPWDAANAIREFLMGSNI"
     gene            15057..15776
                     /locus_tag="PKK36_04940"
     CDS             15057..15776
                     /locus_tag="PKK36_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93940.1"
                     /translation="MDIEKNKSYAVLTGDIVGSTSLPSERRGELHDIMKKGAKDLKRQ
                     FKTALPLEFDIFSGDSWQMLIVEPWHALRIALYFRAYIQAYLVGLDTRIAIAVGSVEF
                     LPKGRVSEGDGEAFRLSGRLLQEKNQTSRMRFAGGRRIFERRGWDAAFYLMDALIMRA
                     WTDKRAKAMLGALQGWPQEQIATLWDPPIRQQSVTEHLQGAAWETIRAVLKEYEHCFG
                     GTKKNASDVPDYLGQEGKQVK"
     gene            15820..16635
                     /locus_tag="PKK36_04945"
     CDS             15820..16635
                     /locus_tag="PKK36_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNR93941.1"
                     /translation="MNILSFIEWQILGSLVVAHLLGDFVFQTDGDVKGKQKLSALKFL
                     KHSMIMALWSYVLVGLWASWRIPLFLGITHFIIDGMKETVTQRKIRKKDGGRLTSGFK
                     IWAFSVDQLLHIMVMVGLALYISKTSILCIKPFWFSQFGTIWTQLLVLVAGLVLTVYV
                     GGVIIGYWVGLFLDQLRNAKEKEPSTDEARGFENGGKVIGQLERALIFLFVVTGQPAG
                     VGFLVTAKSIFRFGELKDAKERMEAEYIIIGTLMSFGWGLTWAWLTQRLLQII"
     gene            16640..17761
                     /locus_tag="PKK36_04950"
     CDS             16640..17761
                     /locus_tag="PKK36_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNR93942.1"
                     /translation="MNATSEFFDEATEQSRLKAEIVSKYFTGWASVIGSSVRQRGPNG
                     KIGYIDLFAGPGKYKDGTKSTPILILEKASANPDLRGMLVSTFCDKDTEQCEELRQAI
                     SSIPDIGSLKYQPKIYNMEIDPQTIREFKGMNLIPSLVFLDPWGYKGLCLELVNALIK
                     DWGCDCIFFLNYLRVNMAIENELFRPHVDAIFGKSRADDLRKILPRLPPLEREQKLLE
                     GLMCALTETHGKYVLKFRFISPEKDRTSHYLFFVTKGFRGYVIMKEIMAKSSSSTVQD
                     VPTFEYEPSGKAKQLSLAFLQTQPIDDLATSLPRDLAGQTLTMQEIYEKHSVGTQYVK
                     RNYRTVLMQLEKEGKITTEPSSEARRKNTFSETVKVSFP"
     gene            17772..>18328
                     /locus_tag="PKK36_04955"
     CDS             17772..>18328
                     /locus_tag="PKK36_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNR93943.1"
                     /translation="MSAKSSIEWTESTWNPVTGCTKISAGCKNCYAERMARRLQAMGQ
                     ANYARGFEVALHEDMLELPLRWKKPQTIFVNSMSDLFHEKVPQDFIMRIFDVMNRANW
                     HRFQILTKRSERLLKMNPELNWAPNIWMGVTVENQACATRIEHLRKTDAHVKFLSMEP
                     LLGAVSHLKLRGIDWVIAGGESGPGA"
BASE COUNT         3920 a         5008 c         5321 g         4079 t
ORIGIN      
        1 gtccgtagtc cgggggcggt gcgcacatcc ggcgggcgtt ttgggcatgc atcctttcgg
       61 tggtccgccg ccaggcgtcg tggtgcgctc ctttcagccc tatgcgcggg cggtatccgt
      121 gattccgggt accggcggcg atcctcgccc gatgacccgg gtgcacaacg atggtttctt
      181 tgagctgact ctgcccggag cacagatgtt tgattaccgg ctgcggatga ccggttttga
      241 cggccataca tgggaactgg atgatccgta ccgctttccg cctcaaatca ccgacttcga
      301 cctctatttg ttcggcgagg gcacgcattt ccgcacctac aagaaaatgg gcgctcatcc
      361 gctgaccatt aatggcgtgg agggagttca ttttgccgtt tgggccccca atgcgcgccg
      421 tgtcagtgtg atcggggtgt tcaatcggtg ggatggccgg cataccccga tgcagaaccg
      481 cgggtccagc ggcctctggg aggtttttgt tcccgcgctc aagccggggg acatctataa
      541 atacgagatt ctcgggccga atgattatct gaccgagcgc gcagacccct atgcttttgc
      601 ctgcgagctc cgtcccaaga ccgcgtcggt catctgggac attgagaagt acccgtgggg
      661 ggacggagac tggatgtcgc gccgtcgcga cacccgctgg catgacaaac cgatcagcgt
      721 gtatgaggtg catctggggt cgtggcgccg caacggcgct cacggtgaat actggctgag
      781 ctatcgccag ctggcagaca cgctcattcc gtatatcaag gacagcggct acactcacat
      841 cgaactgctg cccgtttcgg aacatccgtt cgacggatca tggggctacc agaccatcgg
      901 ctattacgcc cccacatcgc gcttcgggac ccccgacgat ctcaagcatt tcattgatcg
      961 ctgccatcag gagggcattg gtgtcattct ggactgggtc cccgcccatt tcccgaaaga
     1021 cgggcatggc ctcggatact ttgacggcac ccatctctat gaacactccg acccgcgcaa
     1081 gggcgagcac atggactggg gcacgctcat tttcaattac ggacgcaacg aggtccggac
     1141 tttcctgctg gccaacgcca tgttctgggc cgatgtctat catatcgacg gcattcgcgt
     1201 ggatgccgtg gccagcatgc tttatctcga ctactcccgc aaggccggtg aatggattcc
     1261 caaccagttt ggcggccgcg aaaacctcga ggcggtcagc ttccttaaga aattcaatga
     1321 actcatccat ggcgaatatc cgggttttat cacatttgcc gaagagtcca cggcctggcc
     1381 gatggtctcg cgcccgacct atctcggcgg gctcggcttt gacttcaagt ggaacatggg
     1441 ctggatgaac gacaccctgg aatacattca gaaggagccg gtccaccgca agtatcacca
     1501 cgggagcctc acattctcgc ttatctatgc cttcacggag aattttattc tcccgttctc
     1561 gcacgatgag gtggtacacg gcaagcggtc catgctcgac aagatgccgg gcgatttctg
     1621 gcagaaattc gccaacctgc gcgtgctgta tgcctatatg tacgcgcacc ccgggaagaa
     1681 acttctgttc atgggctgcg aattcggtca gtggctggaa tggaactgca accagagtct
     1741 ggactggcat ctgctgcagt tcgagccgca tcagaagctc ttagactgcg tgagggatct
     1801 caaccgcatt tacagggaag aaaaatccat gcacgaggtg gatttcacct gggagggctt
     1861 tcagtggatt gatctgcatg acagcgagca gagcatcatc tccttcctgc gccgcgccag
     1921 ggacggcagc gattttgtgg tctgtctgtt caatctgacg cctgttcccc gctggggcta
     1981 tcgcgtgggc gtgccgaagc cgggactgta ccgggaaatc ttcaacagtg attctgatct
     2041 ctacggggga agcaatcttg gcaatgcggg ggaagtgccg tccgacccga tcccctggca
     2101 gggccagccg cattcactgc tgctgacgct tccgcctctc ggtgcgcttt atctcaagcc
     2161 ttcctgaaaa aaccgggccc gcgcgcatcc ggcatcagaa ctgataagcc acgccggcgc
     2221 caaaggccag atgggagagg ggaattgagc tgttcccgtg atcattgatc cgctgtccgt
     2281 cccgggaata gtggtggctt accgcattca cgtccagata gcgcatgttc aaatccacgg
     2341 cccagtgctc ccggcatttc cagagcaggc ccaggcccgc cgcaaggcag actgcgtctt
     2401 cctcttccgt gtccatgtag cgcgtatggc cgtgataggg ctgtttgttg ccccgggcgt
     2461 aggtgcccga tgtcggatag cccatatgcc accagtcttc catttcgaaa tccacggtcg
     2521 ggaaatgcgc gcctgcctcc gcataaggca cgagaccccg gctcaaaggc atccggccca
     2581 cgagaagcag cgacgggccg ctggacgtga acaccccgtc cacatccccc tggtcatgcg
     2641 tgaccgcctc ggcctccatc tgctcccact ggcaggcaac gccccagtgt tcattcagga
     2701 aatatcggac cgaaaggttg tatggcagaa gggtctgcac ttcgtcaaaa tggtcaattg
     2761 tgcccacaaa gccttccccg gcggcgtcgt cccacttgtc tgaatccagc agcctcacgg
     2821 cggagaaatg cagccccagt tccagatgtc cctggatttt gtccgcatag aaaccttttg
     2881 cgtcttcaag cggaatactc agatccgcct cgccgccctc tgccgctgat cccgtcagtg
     2941 ccgctgcaac cagaaccact ccagcaaaac gaatattcat cgaaaacctc ctcacgtttt
     3001 gaagatatgc tttaatattg aattcgggtc tgcaaatcaa gtcgagaatg gttgtcgcga
     3061 gggggaagtg gtggtggggg atggattcga accatcgaac tcatgagaga acagatttac
     3121 agtctgccgc gtttagccac ttcgctaccc caccgcaacg gttgaaagaa tctactgatt
     3181 ttctccggtt tgtccacccc ctatttctcg cgatctgctc aacctgctct ttacttcggg
     3241 gcgggacatc ttcatcatgc cggcatgaga attattcact tcagcgatct gcatgtctgg
     3301 aggcccgggc tggagacggg tgatctctgg tatccgaagc ggatgctggg ctatgtgaat
     3361 ctcgcgctgc gccggaagtt caaatatccc cccggactgg gacgtgccgt tgtggatgaa
     3421 atcgcccgct ccgatgccga tgccgtcgtt ttcaccgggg acatgaccac cgcttcactg
     3481 ctcggcgagt ttgaggatgc cgcggatctc ttttctccgc tgcttgaaaa atgggaagat
     3541 cgctttgtgg ttctgcccgg caatcatgac cgctataccc catacacgat ctcgcggaaa
     3601 ttctatgaat gcctttttgc ggaccggggg cccgtgaccc gaacggatgt cggcggccac
     3661 tggagcattg tgtcgttcga tgcgtcccgc ccgtacatgg tccgttcgaa cggtatgata
     3721 tccctcgcgc tggccgcgca tctggcgcag attcttcgcg aagtcgccga tgccggacgg
     3781 acatgcgtgc tggccggaca cttcccgtat gaccatcccc gcggatgccg cgagggactg
     3841 cagcaccgcc tgctcggcgc ccggcggctg atcgaggtga tcaatgagtt caagccccct
     3901 gtttatcttc acggccacaa gcatgtccgc tgggttttcc agtcgccgaa aacgccggac
     3961 accgtctgta tcaacagcgg atcggcgggt ctgaaatcgg aagatcccct gaagcgcgca
     4021 ggattcgtga cgattgatct cgatgacaaa ggcgccctgc agggggttct tgcctgctcg
     4081 gcccgccggg acgaagagaa attcgaaacc gtcgctctct gttaagcaag cgccgccatg
     4141 acggtccgga cagagggggg ccttttcagc agttctgaaa agtcgcgttc cgattccaca
     4201 gcgttcttga ggcccgcggc gcagctcgtg ctgcccagca gaatgcctcc ggccattttc
     4261 ccgtcacgga aaaggaagta cgcatagtcg tcgcctgacg ttccttcaac cccggtatag
     4321 cttccgtcct ccggctggac tttgccgacg ctggcgagat ggatgcccag caccttgagg
     4381 gtgttggagc gcggcagccc tccgaacagc gtgtccatgc ccgccgcatt catgccggcc
     4441 atgcggccct gatactgcga ggggccccag atgccgtaca gaactccatg gtgttcggcg
     4501 acatcgcccg ccgcgtaaat gtccggatcg ctggtctgca gatgattgtt cacaacaatg
     4561 ccgcggttca cctccagtcc ggcccggcgg gcaagatacg tgtccggccg cactccggtg
     4621 gccagcagaa caagttccgc gggaacagaa cgaccgtcct gcagttcgac ggcttccgct
     4681 tttccccggc cggtgaccgc gcgggtctgc gcattctttt cgagtgcgat gccgagtttc
     4741 cgcaaatgat tttccagcat ttcgccggcg cgccggttga gctggcgcgg catcagccat
     4801 tcgtggtttt ccagcacggc aacctgaagt ccccgctggg ccagcgcccc cgcgcactcg
     4861 agcccgagaa tgccgccgcc gatgaccacg catcgcggtt tgccggccgt gcgcgcgatg
     4921 atgtcctccg cgtccctcac cgtccgcagc gcaaataccc cgtccatgtc tgtgccttca
     4981 accggcggaa taaagggatg tgatcccatg gcaagaatga gcttgtcata gaaaatctgc
     5041 cgcccattgg ccagcgtgaa gaaatgctcc cgcggcgtga tatgatccac ccgctccccg
     5101 ctgagcagtt ccacgcggtt ttcttcgtac caggattttg ggtgaatggg cagcgtggcg
     5161 cggtcaattt ctccggccag gtagcgcgtg agattgaggc ggtaataggg aagtgcattt
     5221 tccccgcaga tcagcgtgat gcttgatttg ggcgatgcat ccctcaccgc ctccgcagcg
     5281 gaaacgccgg cgatgccggc gccaatgatg gcgacatgaa tggacttctc agtcgggctt
     5341 tgcggagctt tgggctgttt aacggcctcg aactcatctt tagatgctcc gcagaggggg
     5401 cagaaatcgg gcggttcagg gccttcgtgg atatagccgc acacgatgca tcgccactgt
     5461 ttcacttcat cgggctgtgg ctcggccgcc gccggggctt cgtacggttc aaagtccgtg
     5521 tccggcgcgc cgcacagcgg acagtagttc ggaggcacgg ggccctgatg aatataaccg
     5581 cacagtgtgc agcgccacgc tttccggggc tcggtgctca tggtcaggca atctcaatga
     5641 actttgaggc cggaactccg caaatggggc atttgtccgg cgcttcatca tattccgtgt
     5701 tgccgcagac cggacagacg taaattctgc gcgcaggaag gtcgttgccg gccttcacgc
     5761 tgtccagcgc cgcgcggtac aggttgtagt ggatttcttc gacggccaga gcgtatttaa
     5821 aggacatctc ggcctgccgg ctgccttctg caatggcctc ctgcagataa ggaggataca
     5881 ttttctgaaa ctcgaagccc tccccttcga tggccgcctc aagattggcg gcggtgtctt
     5941 tgatcccgcc cattgcgcgc agatgcgcat gcgcatggac cgtttccgcc tcggcggcgg
     6001 cgcggaaaag tttcgcgacc tgcgcgaagc cgtctgcctc cgcttttttc gcaaacgcga
     6061 gatatttccg gttggcctgg ctttcgccgg cgaaagaatc tttcagattg tcaattgttg
     6121 ccatgatgca tccccctgat caggttgtta atgatgggcg caatttagcg catgcgcccg
     6181 tcaatcgcaa ggcgaagggg tgaaaaaaag tcggcgggtc ttgtgcaggg agcataaacc
     6241 gtgcattctg cggaatcatg agacggtttt gcatgtggct ggcgctgatt ttgtgtatgc
     6301 acggcgggtt tagttctgcc gccgcggatt ctgtccgtgt gccgcgcaag acacctgacc
     6361 gcgagatcac gatgcgggtt accggctact gcccctgccg ggaatgctgc aactggaaaa
     6421 gaaactggct tttccggccc gtgattgctt caggtcctca gaagggccgc ccgaagaaag
     6481 tgggcatcac ggccagcgga aagagggccg gtccgggcac cattgccgcg gacgcccgcc
     6541 tgttcccgtt cggcacgatc atgtatgttc cgggttacgg ctatggcatc gtggaggacc
     6601 ggggcggggc catccggggg catcacattg atctgtttta cagaagtcat gccacggcga
     6661 aaaaatgggg atcaaagaaa cttcgtgtgc aggtctggta tccgccgaaa acatcccgga
     6721 agccgtcgcg gaaaaagtga catcacagcg cgtcgagaat ttcctgccgc tgctggttca
     6781 ggtcgtggat ttccctgcgc cagaaagact ccgcgcccca gtccggcgca aggcgcgcga
     6841 agcctccgtc cgccttctgc cgcgcgcacc acgccgtgaa gtgcaggaac cgcatggcgc
     6901 gcagcggctc gatcagccgc agggtctggc ggtcaaaatc ccggaaagtt tcgtagcctt
     6961 ccagaagcag attgaggtca ggagttgaat gcttcaggtg cccgggcagc agcatccaga
     7021 tatcctgaac cggcggcccc agggccatgt cgtcaaagtc gatcagcgcc agcggctcgt
     7081 cgggccgctg cagaatgtta aggcggtgca tgtcgccgtg aatgcggatt ttttcgaccc
     7141 cgtcaaagag cggttcaatc agatcaagaa tgccccgggc cgcctcctcg tagttcttcc
     7201 ggagctggcg cggcacggca tccgcatcga gaatgaactg aaaatgcatt gccgccgact
     7261 gccgtgggcc gatttcgatc cggtcgcgcg gcggatgtgc ggcgcccaca gagtgcacgc
     7321 gtgccagcag gcgcccgagc tcgcgccact gggcctcatt gggctcatcc agcggcctgc
     7381 cgccgcgctt gggaaatatc gcgaagtacg tgccgtgata ttcctgcagt gcttcgccgt
     7441 cggcgccgcg catcggtgcg atcaccggaa tttcctgcgc gcgcagttct tcaagcagat
     7501 cctgttcgtc cagaagggcc tcgcggctcc agcggtttgg gcgatagaac ttcacgacga
     7561 cccatgagcc gttgtccagt tcgacgtcat acacgcggtt gatgtaactg gtgagcgggc
     7621 ggcacatgtt cgtgcagcgc gcgccgagcg cggtctcgac aaggtgcagc acggtatccg
     7681 gtgtcaggtg cgaaaagtcc ggaagcgcgc tctggttctg atctggcatg ccgggaacta
     7741 tacagccccg gccgggtgcg gcaaagaaac tttgcggcgt catcagggaa ttttgcgcgt
     7801 gccgatggtg taaaagacca tgtccgcggt gttggtgaaa aacagggtga tgtctccgcc
     7861 gccgcccggc acattcagcc ccagcggcat ccatccgccc gtcaggttgg tggagacata
     7921 aatgtcataa agttgtcccg ccacggaatt gctgaccacc agcgcggttt gcccggcggc
     7981 caccccggac acatccggcg gcgcgaccgg ctcatcgccg ggcgcgcgcg tgaccgccac
     8041 ttccgccgcg ctgggtatgg gctccagcgg atctccgtca atggacatgt ccagcacaaa
     8101 caggtcgcgt tccggcccac cgcctgcgct ggcgttccag atgcggacct ggcggatcag
     8161 catttcggag gagattccca gattgccctc gaattcatcc ggtccggccg tgagccggta
     8221 cgtgctgccc gaggtcagtt cgaattcgag cgtcaggccc gaatttgacc aggggatgcc
     8281 ggtgtttcgg gttgtgagtt cgtcgttgat caggtaattg gtgccgcccg ccacaaaaag
     8341 gaactcgaac aggctctgtc cgaaatggtt ttcgagcgcc acgccgatcg aggagtccgg
     8401 atcatccacc ccgccgtttt ccagccggat ggtgaacaca tcgccgggat tcaggcgcgc
     8461 ggcaaacggg cgtaccgcct cgcagaatcc gccgctgttg gcccacatgc cccaggcccg
     8521 tgatccgctg tcgagataga ccgtgttggt cgccatgaag tgtccggagt tcggcgtggc
     8581 cgtcaggttc catcctccgc cccatgctgc tcctccgttt gctccgtcgg gccagccggc
     8641 atcgtagacg acattggtcg cgctgtcgtt cgctcccgcg ttcagattca ccgtgtcccg
     8701 caggccgctg atgtgaaaga ggttcacgcc ggtgaggagg ggaatcccgg acaccagcca
     8761 gttcgcggcg gaagggatgc tcccgccgct gcccgcgtgg ctgttggtcc agatcaggtc
     8821 gcccgccagt ccgtaagaag agccttctat gtttatcaca gcctgggtgg ccgcgacatg
     8881 caggtcggcc gcgggatttg tgatggcaat gccgggtgac tgggtgttgt agcagccgga
     8941 cacgaaaaca gtccagtccc ggctgtcgtt gttgtcccag acgcggttgg tgccttcagc
     9001 gccgtcgtgg aaaacgaaat cgagctgata ggtgctgtcg gggatgtccg cggtgacaga
     9061 ccatgcgccg gccgcggggt ttgtcatggc cagcgtctgc acatcctgcc atccgttgcg
     9121 gccgaaatgc acaaagacgg cttccgccgt gctcagaacg ctctcccgcg cctcgtacgt
     9181 gatggtgacc ggcacgcatc ccgccggcac ggccggggaa accgtcacca gcgcgggcag
     9241 gctggcgcat ccgctgacat tcacccgcca gttcatgctt tcgttgttgt cccagacgcg
     9301 cgattcctcg gcggctccgt catgaaaaat caggttgagt tcatgagtct gcgaggggat
     9361 tgaagcggtg acaacccatg tgttgtcgcc ccggttggtc atcacggttt cctgaatatc
     9421 aagccagttg ttccggccct gatacagcac gacattggtt gcgcccgcca gcggacctcc
     9481 ggccggatca taggaaattg tgacatccac acagccttcg ggggcagggg gatcaaaggt
     9541 gaccgcgccc gcgccggtcg gaggcgtctt tgagaaaatc tgcaggctgt acatgcccat
     9601 gtccaccgcg gccatggccg gacttcccga tgccgtgacc tgcgtgaagc cgatgtttcc
     9661 gaagtctgct ccgtattccg tgctgtcgct gttgaagtgg ctgtaccaga cgccttcgga
     9721 ggggaactcg atgagatagg atccgtttgt gaaagtgttc gcggagaaat ttgccgcgac
     9781 aactgcatcg tccgcgccgc cgcccgcgtc ccagcggaca taggcgatga ccttgcgggt
     9841 gtcgtccacg tggtgaacgg ttatgccggt gcccttgagg ccctgcatgc cgccccgcag
     9901 gttccggcgg ctgcggatca ggtcgcgata ggcgcgcacg atgcccgcat gcgtgttggt
     9961 cagggcccag cggagcgatg tgctggaact gaaggtccag tcctcgttca tttcctgtcc
    10021 ctggaagatc atgggaatgc cgggctttgt catgacggcg gcggcggcca gaagctggcg
    10081 cttgcgggcc cagatgcccg ccggatcagc gccgtcaatc gcgcgcggaa ggcgcacggc
    10141 gtcgttcagg tccccgacat agtcgtgggc ctcggagaag atcacgcggc tcccgttcgc
    10201 gttggccaga agatgatccc gaagcgcaga catattccgg tttgcatcgc ttccctgcgt
    10261 gacctgccat tcgatgtgat cgtgaaactg cacatcccac atgctttcga agttcatgct
    10321 gtagtcgaac gcgttgtctt cggatatgcg gaacacgttt ggatgattct ggctcagctc
    10381 ccagttgatg tcccgaagca gatattctcc gtccgcgttg tgattgtacc cgtcgttgta
    10441 atagatgatg ttgaacacgg agtcccagcg gaagccgccc acgcgatatt cctcgacgaa
    10501 catcatgatc tggtcgcgga tgaaatcgcg cacctcggtc cggctgtagt cgggacgcgt
    10561 gcttccccac caggtgtagg ccttgccgtc cgtggaattg tagaaataga ttccgcccag
    10621 cccgttctgg ctccatccgt cgaactgcca gagggccaga tcggtcgggc cgtagtgatt
    10681 gtggaccaca tccgtgaaga cggcgattcc ccgctcgtgc gcggctttca cgaaccgctt
    10741 gagcgcatcc ggaccgccca gcgcactttc aatggcgaac aggtccgacg gattgtatcc
    10801 ccagctccgg tcggtggcga attcgttcac cggcatgagc tggataacat taatgcccag
    10861 gttccggagg tgatccagcc gttcgatgtt cttgtcgaac gagctgggaa tccagtcttc
    10921 ggcgttgaag gttcccggat gcatctgata aagcacggca tccttgcggt ccggcatggg
    10981 gatgggggtg tcgccccaat cgaaggcgtc cggatcataa atgatggagt tgcctgtcga
    11041 atgcgtcacg cggcgcgcgc gcggatcacg ccgccagatg ctgccgttga gaaaatactg
    11101 atattcctgt cccgggcgcg cggacgggat gtccacggac cagtatcctg tggaaccctc
    11161 actcgtcatg gcggtcgtgc cccagccgtt gaagtcgccg cgaacggcca cggtggaggc
    11221 gttgggcgcc cagacgcgaa acgtcacgcc gttgctgcga accagtgacc cgagttctcc
    11281 cgggaaattc gtggactgcg cccgggtcgc ggccgccatg agagccgccg ctgaaaaaac
    11341 agccagcttc cggcacagga tgtatgatga ctggttcatg cagaccggtt ttttaatgtg
    11401 cagaacaata accccgcaat gcccagatgt aaaacgttta actccccgaa agcgtgaatc
    11461 cctcctcccc gtgaaaaaac actgccgccg gcgtatattc atttgagggg ctggtgggat
    11521 catgactttc aggcgggaaa aaggtttttg gatcactgaa ctgaaggagg acgactatga
    11581 acctcctcct gtacatgttt tatccgctcg ttggattgat cgagtggtat ctggctttgc
    11641 gcagaacttt ggcctgcgtc cggagggaac gcgctgttct tgttacaatt gtgtttatcg
    11701 aaaatttgct gggattggcg gttttgcaga attttataac ctccggtaac tggctgatcg
    11761 ccattttcta ttccatgggc gccggtctcg gcgcgctctt gagtcttcgc atcgcatccg
    11821 gcgacacctc caaaacctga ttccggctgc gtaaggacag agactgggaa agtctttcgc
    11881 tgattggcgg gcagtgtctt ttcccgggga actaaacttg ttatttcgct gtcattgatt
    11941 aaactccaag ggcagtgaag ttatgggaaa agacgttgtc atagaaaatc cggttatcaa
    12001 ttcacctttc gaggagccct gtagacattt taagtttgat gatgaaggga taaccaatga
    12061 aattgtccct aaaagacggg tcagtgctta ttttatcccg attgctcagc ctcgaaaacg
    12121 aagccgggat cagcttgtct ttgatacaga gtggactaag gatcgcctcg aagaaaacat
    12181 cacgataaac aggatccgat cccgcgtggc ggtctggcgg cagggcggtt atcccggtgt
    12241 aacaagaacc acggcccgtt tgctcgaata ctggacaaat ccgcaaaggg aaaagaagct
    12301 gtttttctgc cagattgaag cgcttgaaac gctcatttac ataaccgaag ccgccaaaaa
    12361 atatggcgac acctggattg aaaatgaact caaatcggcc aatgagggtg cgaatcccgg
    12421 tttgttccgt ttagccctta aaatggcgac cgggagcggg aaaacggtcg tcatggggat
    12481 gatgattgca tggcacgctc tcaacaagat tgcgaatgcg caggacaatc ggttctccga
    12541 tacgtttttg attgtgactc ccggcatcac aatccgtgac cgcctgcgcg tgcttcttcc
    12601 caatgatccg ggaaattact accgccagcg cgacctgctg ccggcatata tgatggatga
    12661 acttggacag gccaagattg tcatcacgaa tttccatgca ttcaagcagc gggagaaaat
    12721 tgctgccggg aagttgacga aatcaatttt gagcaccgga gataatggag cttttacaga
    12781 aaccccggcg cagatggttc ggcgagtctg ccgggagctt ggtagcaaga aacagattgt
    12841 ggtcatcaac gacgagtctc atcattgcta tcggcacaaa cccgacggcg aggaagttgt
    12901 cctcaagggc gatgagcgca aggaggccga ggcccgggag gaatatgccc gcgtgtggat
    12961 ttccggtctt gaagcggtgc gcgaccaaat tgggctgaag gtcgtttatg atctttccgc
    13021 cacaccattt ttcctgagag gttcgggtta ttccgaaggg acattgttcc cctgggtggt
    13081 ttccgatttt tcgctgatag atgccatcga aagcggcatt gtcaaagttc cgcgtgtgcc
    13141 cgtggccgat gattcaatga agggcgatca gccgacctac cgcgacatct ggacgcgtat
    13201 ccgggagggt ttgcctaaaa agggccgccg gaccgatgcg gtcacgggtg aacccaaact
    13261 gcccaaagag cttcagggcg cgctgcacag cctctacgac aactacagga aatattacga
    13321 actttgggaa aagaatcctg acgcaagagc caataatcag accccgccag tgttcatcgt
    13381 tgtttgcaac aacacaaatg tatccaagct ggtattcgat tatgtggctg gatgggagaa
    13441 aacgatttca aaagaaaaaa ccgcgctcgt ccccggcgca tttccagtct tcagcaatgt
    13501 tgtggatggc cagtggtctg ttcggcccaa taccatcctc atagacagcg aacagcttga
    13561 gtccggcgag gccatgagtg cggatttcaa atccgccgcc gcccgtgaaa ttgaggaatt
    13621 caaggcggaa taccgtcagc gttttccggg tcgcgacacc gaaaagctta cggatgaaga
    13681 cctcctgcgg gaagtgatga atactgtggg caagccagga aaactcggtg agcacgtcaa
    13741 atgtgtaatc tcggtttcga tgctcaccga gggctgggac gccaataccg taacccacat
    13801 tcttggggtg cgtgcttttg gaacgcagct gctttgcgag caggtggtcg gacgcggtct
    13861 gcgccgcagg tcctatgtta cggaaaaccg cacgattgat gtgggcggga agcctgttga
    13921 gatcaatgcg ttcccgacag aatatgccga ggtttatggc gttccgttct cgtttatacc
    13981 ctgttccggg tccaccggac cggggccgac cccgccagtc ccgaccacac gtgtacgggc
    14041 aatggacgag cgcaaaaact gcgaaatcac attcccccgt cttaccggtt acaggtacga
    14101 attgccggga gaaattctga ttgcgaaatt cacggacgat tccaaaataa cgctttcaac
    14161 ggaagaagtg ccctggaaaa caatcaatgc gccgatcgtg ggtgaaagtt cggtgcatac
    14221 gctggaggat ttaaagtcaa ggaggcttca gcaggttgcc tttctgctgg caaaactgac
    14281 gcttgagaaa tatttccggg cggattcggg gggtatcacc aatggaggga cgctctccgt
    14341 cgcgtccgag gttccgacag agcggaaccc tccaacagat gtcaaagcat ggctgttccc
    14401 gcaactgctt gaaatatccc gttcctggct tgatacctgc gtcaagtgca aggataacac
    14461 cttcccgcag cttcttctct tgctggaata cgcccatgat gcggcggatc ggatatatcg
    14521 ctccattgtg cagggaacgg agggcgaaaa aacgcttctg ccgatcctgc agccatacga
    14581 caccatcggt tccaccaaat ttgtggattt tgataccacg cgcccggtgt ggagcactcg
    14641 cgccgacaaa tgtcacgtct cgcatgtggc ggccgacacc aattcatggg agcagaaact
    14701 ggcccagacg ttggaagata tggacgaagt gatctgctat gtcaaaaacg agcatgttgg
    14761 cttcaccatt ccgtacacgc tgaacggcga agaacacagt tatattcccg acttcattgc
    14821 ccggatggat gacggacatg gggcggatga cccgctgaac ctcatccttg aagtcaccgg
    14881 cgagaagaaa aaagacaagg cggcaaaggt tgccacggca aaatcccttt gggttccctc
    14941 cgtcaataat cagggcgagt ttggccgctg ggggtttgtt gaaataaccg atccgtggga
    15001 tgcggcaaac gcgattcgag aatttttaat gggaagcaat atctgaggtg cagaaaatgg
    15061 atattgaaaa gaacaaatct tacgcagttc tcaccggcga tatagttggc tcgacaagcc
    15121 ttccctccga gcgtcgtgga gagttgcacg acataatgaa aaaaggtgca aaagatctta
    15181 agaggcagtt caaaacagcc ttgccgttgg aatttgatat ttttagcgga gacagctggc
    15241 agatgctgat tgtggaaccg tggcatgctc tccggatagc actttatttt cgtgcgtata
    15301 ttcaggcgta tttggtgggg cttgataccc ggatagcaat tgctgtcgga tctgtagaat
    15361 tccttcccaa aggaagggtg tctgagggtg atggcgaagc cttccgactc tccggacgtc
    15421 tgttgcaaga gaagaatcaa acgtcgagaa tgaggtttgc cggtggccgt cgtatttttg
    15481 agcgaagagg gtgggatgcg gcattttatc tgatggacgc tttgattatg cgtgcatgga
    15541 ccgataaacg cgcgaaggcc atgctgggag ctcttcaggg ctggccccag gaacaaattg
    15601 ccacgctatg ggatcctccc attcggcagc aatctgtaac agaacatttg cagggtgcag
    15661 catgggaaac aattcgggct gttctgaagg aatacgaaca ctgttttggt ggaactaaga
    15721 agaatgcgtc tgatgtgcct gattatctag gccaagaagg caaacaggtt aaatagcctg
    15781 taattttaaa atacaggcta atcagcctgt aatagattta tgaatattct ttcatttata
    15841 gagtggcaga ttttgggaag tcttgttgta gctcatctgc tgggagattt tgtcttccaa
    15901 actgatggcg atgtgaaggg gaagcagaaa ttgtctgctt tgaagttctt gaagcacagc
    15961 atgataatgg ccctgtggag ttatgttctc gtgggcctgt gggcttcttg gcggatacca
    16021 ctattccttg gcataactca ctttattata gatggcatga aggaaactgt cacacaacga
    16081 aagattagaa aaaaagatgg cggcaggttg acatcaggat ttaaaatatg ggcattttcc
    16141 gttgatcagc tgttgcatat aatggtgatg gttggtcttg cactatatat ctcaaaaact
    16201 tcaatccttt gtatcaaacc tttctggttt tcgcaattcg ggacaatatg gacccaactt
    16261 ctggttcttg tggcaggtct tgttcttacg gtttatgttg gtggtgtaat catcgggtat
    16321 tgggtggggc tgttcttgga tcaactacgc aatgctaagg agaaggaacc atccacagat
    16381 gaagcccgcg gcttcgaaaa tggcgggaaa gtcattggcc agcttgaacg agctctaatc
    16441 tttctgtttg tggtaacggg gcagcccgca ggagttggtt ttctggtcac ggcaaagtcc
    16501 attttccgct ttggcgaatt gaaggacgca aaagaacgca tggaggctga gtatatcatc
    16561 attggcacac tgatgagttt tggctgggga ttgacatggg cctggctcac gcagcgcctc
    16621 ttgcagatta tttgagatca tgaacgcaac cagcgaattc tttgatgaag caacagagca
    16681 gtcgcggctc aaggcagaaa ttgtatcaaa gtatttcacc ggctgggcct ctgttatcgg
    16741 atcgagtgtt cggcaaagag gccccaatgg gaaaatcggg tacattgatc tttttgcggg
    16801 gccgggtaaa tacaaagacg ggacgaaatc aacccccatc cttattcttg aaaaagcgtc
    16861 agccaatccc gatctgagag gaatgcttgt ttcgacattt tgtgacaaag ataccgagca
    16921 gtgcgaagag ttaagacagg ctatctcttc cattccagat attggttcgt tgaagtatca
    16981 gccgaagatt tacaatatgg agatcgatcc ccagacgatc agggaattca agggtatgaa
    17041 tctcatcccc tccttggtgt ttcttgatcc atggggatat aaaggtttgt gtttagagtt
    17101 ggtcaatgcg ctgataaaag actggggttg cgattgcata ttttttctga actacctgcg
    17161 cgttaacatg gcgattgaga acgaattgtt ccggccccat gttgatgcta tctttggcaa
    17221 atctcgagca gatgatctcc ggaaaatact tcccaggctg ccgccactgg agcgagagca
    17281 gaagttgctc gagggtctta tgtgtgcttt gacggaaact cacggcaagt atgttcttaa
    17341 gttccggttc ataagcccgg agaaggatcg aaccagtcac tatctgtttt ttgttacaaa
    17401 gggctttcgc ggttatgtga taatgaagga aattatggcg aagtccagtt cctcgactgt
    17461 tcaggacgtg ccaacgtttg aatatgaacc ctcagggaag gcaaaacagc tatcattggc
    17521 ctttctgcaa actcagccga tcgatgattt ggccacatcg ctgccgagag atttggcggg
    17581 acaaacattg acgatgcagg aaatatacga aaaacacagc gtcggaacgc aatatgtcaa
    17641 aagaaattac agaacggttt tgatgcaatt ggaaaaagaa ggtaaaatta caacagaacc
    17701 ttccagcgag gctcgccgga agaatacatt ttcagaaaca gtaaaagtta gtttcccata
    17761 ataggttaaa tatgagcgca aaatcatcaa tcgaatggac cgaatcaacg tggaatccgg
    17821 tgacgggctg cacaaagatc agcgcaggat gcaaaaactg ctatgcggag cgcatggccc
    17881 gccgcctgca ggccatgggt caggccaatt atgccagggg ttttgaagtg gctctgcacg
    17941 aggatatgct ggaacttccg cttcgctgga agaaacctca gaccattttt gtcaactcga
    18001 tgagcgattt gtttcacgag aaggtgccgc aggatttcat catgcggatt tttgatgtga
    18061 tgaaccgcgc aaactggcat cgcttccaaa tcctgaccaa gcggtcggaa cgactgctga
    18121 aaatgaatcc ggagctgaac tgggcgccca atatctggat gggggtcacg gtggaaaatc
    18181 aggcctgtgc aacgcgcatt gagcatctgc gcaaaacgga cgcgcatgtg aagttccttt
    18241 ccatggaacc gcttctgggt gcggtatcgc atttgaagct gaggggcata gactgggtca
    18301 ttgcgggcgg tgaatccggt cccggcgc
//
LOCUS       DAOQWR010000041        18215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3461_length_18215_cov_6.079350, whole genome shotgun sequence.
ACCESSION   DAOQWR010000041 DAOQWR010000000
VERSION     DAOQWR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18215
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3461_length_18215_cov_6.079350"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2521)
                     /locus_tag="PKK36_04960"
     CDS             complement(<1..2521)
                     /locus_tag="PKK36_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93944.1"
                     /translation="MKKHQCAAFAAVLTVFAGQPQARAQVPSMINYQGRLVDGGMLVN
                     GNVELVLRLYNDASAGTLEYEDSNTVTAVDGLYSTFIGDNTLSGSLQDALTNTEIWVE
                     AEVDGTILLPRQRLVSVAYALVSGGGGDFVETAGDTMTGALNINADAGALVIGSTGMA
                     VRIGNSAVATTSGTAIGLDANAASAGVAVGRLANGADQGTALGYNANAYDRSVAIGIN
                     SAAVNGESVAVGNGANGSSVGVAVGHGANAYSGGAALGRGAVGRVSGVAAGDYANGYN
                     GGAAVGGEANAANSGVAIGRYANGSLFGTAVGRNSLAYNEAIAVGYAATGTLRSVVAG
                     YTASASDYGVAVGNRAFANKESIALGNQANAAGTDRIAIGNHVVNTQEADTVVMRGSL
                     YLDGGTSVFYRSTFGSGEWQEFSGSGGGEPEADPIWSSASNFYYQKTESDDFYVDAAG
                     DTMTGELIVNAEGGALVIGSTGLETRVGGNAAATGGGTAVGREANAYSTGTAVGYGAN
                     GSDEGTAVGHTAAAAENGSAMGYRANGSLSGAALGSFAIAPDDGVAVGAGANGSYLGA
                     ALGREANAAGTNIAIGAFANAADGFSRTAIGASLTNEIDNSTAVRGSLYLDGGTSVFY
                     RSTFGSGAWQEISAGGGSITETDPVFAASAAFAISALNTSEWSEAHGWGDHSEAGYAT
                     GTPVYAETDPVWVAERSNYATGAPLYEYGETDPVWDAQKADYATGTPVYVETDPVWDA
                     EKSGYATGTPLYVFTETDPVWVAERSNYATGAPLYEYTETDPVWDAQKADYATGMPVY
                     VETDPVWDAEKSGYATGTPLYAFTETDPVWTAASNDIQSQIS"
     gene            complement(2700..5192)
                     /gene="gyrA"
                     /locus_tag="PKK36_04965"
     CDS             complement(2700..5192)
                     /gene="gyrA"
                     /locus_tag="PKK36_04965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR93945.1"
                     /translation="MLENNQNHLINIEDEMQRAYIDYSMSVIIGRALPDARDGLKPGN
                     RRILFAMKERGWIHSKPFVKCAKVVGEVIGNYHPHGDSAVYDTLVRMAQDFSMRCILI
                     DGQGNFGSIDGDRAAAYRYTECRLKAEAEEMLADIEKSTVEMRPNFDESLMEPAVLPS
                     RIPNLLVNGSTGIAVGMATNIPPHNLAEIADGMILLIDNPDIPVAEICKVVKGPDFPT
                     GGVICGLNSIREMYETGRGKLKVRGRAGIETANNGRESIIITEIPYTVNKASLIEKMA
                     HLVNEKTIEGISDIRDESGKEGIRVVVEIKRGAVPKVVLNSIFKHTQMETTFGAIMLA
                     IDHGKPRVMNLKDLMTCFIDHRVDVITKRTEFDLAKAEARAHILEGLKIAIDHMDAVV
                     KTIREAKDRDDARVKLMARFGLSELQANAILEMRLYQLTGLERDKVENEYLEIIKLIS
                     YLRDLLASPAKILGVVKEELLEIKERYGDKRRTDIVADAGEINIEDLIADERCVITVS
                     HTGYIKRVPTSSYRQQKRGGKGVVGMDTKEEDYVEHVFMATTHDYLLCFTQAGRMHWI
                     KVYDIPEGSRTARGKAIVNLLQVGTDEKLASMLRVREFSDTEHLVMATRKGIVKKTNL
                     SAYSNVRAGGIIAISIDPDDELIGVVKTVAGQDLILTTRNGMSIRFDEGELRDQGRAT
                     RGVKGMTLSKDDIVENIAVVDPSATFISITENGYGKRTNFDEYRRQSRGGKGIITIRT
                     TERNGKLVAALSVKDQDALMLISQGGQMIRFGVSDLRVISRNTQGVRVINLDEGDRLI
                     SATPLEPEVEDEEEDKETAGTPVSETPPAGDQ"
     gene            complement(5218..7683)
                     /gene="gyrB"
                     /locus_tag="PKK36_04970"
     CDS             complement(5218..7683)
                     /gene="gyrB"
                     /locus_tag="PKK36_04970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNR93946.1"
                     /translation="MSKETEDAKQKAGAKYDATTITVLEGIAAVRKRPAMYIGDTSVR
                     GFHHCVYEVVDNSIDEALAGYCSNILVRVNADGSCTVNDDGRGIPVDMHATEKKPAVE
                     VVLTTLHAGGKFDNESYKVSGGLHGVGVSCVNALSEWLEVEVRRNNLVYHQRYERGVP
                     VTKLETIGTTKGTGTKVTFKPDPQIFSTSEFNWDTLAVRLRELAFLNKGILIKLSQEE
                     PAREEVFQYSGGIREFVTHLNRAKNPLHPKPIFFEREKDGIQAEIAMQYCDTYKETIY
                     CFANNINTIEGGTHLSGFRSALTRTVNQYAKTNKLTKNEAGMSGDDIREGLTAVISVK
                     LPQPQFEGQTKTKLGNSEVEGIVASIVNEELSTFFEEHPSVARRIIDKAVVAARAREA
                     ARKARDLTRRKGALESSSLPGKLADCSERDPALTEIFIVEGDSAGGSAKQGRDRRFQA
                     ILPLRGKVLNVEKARLDKILSNEEIRTLITAIGTGVGEDDFDIAKARYHKIIIMTDAD
                     VDGAHIRTLLLTFFYRQMPQLVEKGYIYIAQPPLYKIKRKKREQYLDSDEALTKILLD
                     LGSDDVKLLNPAGEEVLPDKALIRLLELLSEIETLGNRIQRRGAVFSEFLTRRDPKTG
                     RFPQYRVAITYRDTSETEHFYAYDERELRKLREEAEKKLGLQLEIFTNGENVESKPDQ
                     SGLRWVEFYGAVDLAKAVAKLEKMGFSLDQYQPAEEPIYFLADEKDQRTPIGSLPDLL
                     DAVRNLGRRGLVIQRYKGLGEMNPEQLWETTMHPENRKLVKVVLEDDVRADEIFTILM
                     GDDVGPRREFIEQNALNVQNLDV"
     gene            complement(7893..8741)
                     /locus_tag="PKK36_04975"
     CDS             complement(7893..8741)
                     /locus_tag="PKK36_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93947.1"
                     /translation="MKQHIQVLLAISFFTVCSVALAQKSFEAYPLGNNDPAALTETAK
                     AIVGPEGAVVLDGKGNRLLVIAEAEQHKSLAEMFSKIPVIGKNVFIEVRFTTVGRTKD
                     SGASVEAGGEISKTGSKIVIKPQVHHMTTETGGETVQSIMAASGSAARIQVGTSVPMM
                     EWFVDYGWRWAAIHERMHWNEVGAFLTVEPTIIGDGPEIKLVLTPELSGLDYDKPCCV
                     KFTRASLEVTALNGVPVRIGGLEKDEEFYSRFLLGGSSQEDSCALDITLTAWITDASG
                     RRLPAE"
     gene            complement(9254..10161)
                     /gene="epmA"
                     /locus_tag="PKK36_04980"
                     /pseudo
     CDS             complement(9254..10161)
                     /gene="epmA"
                     /locus_tag="PKK36_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942397.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
     gene            complement(10191..10751)
                     /gene="efp"
                     /locus_tag="PKK36_04985"
     CDS             complement(10191..10751)
                     /gene="efp"
                     /locus_tag="PKK36_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNR93948.1"
                     /translation="MYNASDLRKGLKVEIDGEPYMITEFNFVKPGKGQALYRCKLKSM
                     VTGSTFDKTYRSVDRIDKCNLEERDMIYSFQEGDHYVFMDAETYEQVHIAAEVVGDKK
                     YFLIEDMEVDILFHNDRPIEIMLPNFIEKTVAHTEPGARGDTATNVLKPATLDNGYEI
                     QVPLFVNEGDRIKIDTRDGSYAGRVS"
     gene            complement(10834..12411)
                     /locus_tag="PKK36_04990"
     CDS             complement(10834..12411)
                     /locus_tag="PKK36_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR93949.1"
                     /translation="MVDFFKKSKDGQIEHLAETAPVEGAHPPQMHRRREERADRGPSR
                     HKRREAPPQKSAAPAKEAPGPHPDVQVDTPHPAAAKKRRRRPRRRRQPEGAPATEGKN
                     LRHDEPRPAVVPPPARDEKPWDISVYDVPPQEGKTRFHDLGLPPELMRALYDLKFQYC
                     TPIQQQILPHALKGSDVSGRAPTGTGKTAAFLLSVFTHMLRKPITDQRRLGTPRCLVI
                     APTRELVLQIQRDAEALGKYTGLRSVAVYGGDSSRNQALQLEKGPVDILAATPGRLLD
                     FKNQHKIHLGHVETMVIDEADRMLDMGFIPDVRKIINSTPPRHERMTMLFSATLTPEV
                     LRLSAQWTRDAVTIEVEPDQVAVDTVNQIVYIVRSSEKFNLLYNILKADNPQRVIVFT
                     NRRDEAQRLSDRLNRRHFPCALLSGALAQNVRTRMLNEFKEGKYRILVATDVAGRGLH
                     IEDVSHVINYCIPREAEAYVHRIGRTGRAGKSGVSISFADEEDSFYIPEIEKYMGRPL
                     PCTYPDERYLTDPDKKG"
     gene            complement(12539..13906)
                     /locus_tag="PKK36_04995"
     CDS             complement(12539..13906)
                     /locus_tag="PKK36_04995"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNR93950.1"
                     /translation="MKAEQKSRGVEGFLLDLQAAIQDSRFYGPKHALTEAAAVRVRAS
                     LHEAMAGRTEIVIGVIGGELAFGNKPLYDLTRHMSSLIAALEEHKVEKLTFMGEVPLA
                     DIRSFIELLTCRSGEMTTAAGELGSITISGIGLEASPSGSVLSAGTVEETYREGPRLL
                     GDIGRALHDGLPVNTRAANTFIQKVMKGFLGNKAPFLIATSLKKHDEYSFVHSINVAV
                     LSLAQGEALGLPKSALADLGLAGFLHDSGKIALAADILRKKGKLSESEYEKVSQHPLD
                     GAKLLLQTPDLSPLVPTVSFEHHLRYDGRGYPRKVFGTELCLASMIVSISDVYDALRS
                     RRPYRGGLAPELAFKEMIKEGGGLFHPSLLGVFFRAIGIYPPGSLVFLDDGSVGIVIA
                     PNPADIRRPVVEIWYAPSGETLRVPQVVNLTEKKPGRNEYLHMIVRSVPPEAGHKIPA
                     RYRID"
     gene            complement(13903..15960)
                     /locus_tag="PKK36_05000"
     CDS             complement(13903..15960)
                     /locus_tag="PKK36_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93951.1"
                     /translation="MGHKVRGAMVEENSGSDKTAAFMELVRFINTAIRNSAMYPAGHP
                     MLADSLKSLKAALDGWLGSEGKLDMAFTRSAVLVGGKPFKEGNPVLAGLARHFHQRGL
                     LALTIRPGVVASEVSSLLAAIKDPPEIIARSGGVSSRLRRCPHLSAKQIDYSTLLTRS
                     VDAAGGGRDIDIVQLLCDADKRARAGFPSAAVIEEICAALAGGRKFSAAINEIFTSPG
                     GHRGEAAKNLWRVMGRVVRFCKDLPPEKAARTLRHLSTVLAGLNPEALDALFSEQAAE
                     EGVDDLQKAALPAVPDAALSEMISSHITREGKVDSSLMSLFLRLSDRAGASPDLAQSV
                     AGKLSGLDHDRLATVQGALEDALKTAARNEFMSQVYQLTMNTLAEKERQTIQTDAAVA
                     ALVANFQLMCEKNRAQDEKVRLLLNILWFEENPRTFREFSGRLLDELPRCSKNTRPEM
                     TRDALKVFWEKTCPGVDPAIAREAVSSLYRLGPLAGSEALAALIPGSSPSQIEEIAHL
                     LSETYADGGKALLNLAINRERGPAGNEICAALAAMKLSDEQAADLAKKAVREKDPAVR
                     RVYLCVLAGSGDQTVIGRLFRFLRGGVFSHRILLELIDICGQGKIAASVPFLSGLLKH
                     HTLPMPGRLGIVEAVVESLCRIHTSAAAAVLQKTIQEGNRNVQRACRRVMPLTGARRG
                     PAA"
     gene            complement(16037..17179)
                     /locus_tag="PKK36_05005"
     CDS             complement(16037..17179)
                     /locus_tag="PKK36_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNR93952.1"
                     /translation="MCADTYTEVSKQSWFSRLGGAVKGILGGLLLIIVAFPLLFWNEG
                     RAVKRYKTLQEGAGAVISVSCDSVDQNNSGKLVHMTGLAQTEEMLTDPVFGVSRKAIK
                     LRRSVEMYQWDEDAESRTKKKMGGGTETVTTYTYKKVWSESLIDSGKFRQSGHDNPGA
                     MTYQTYELVAQSVTVGAFSLSTSLIAKIRAYSAVNPAEGEGPADSAAIRRQPGGFYIG
                     ADPAAPQIGDLRVAFSAVEPQVISLVSGQQGVSFEPYVTKNGGTIQLLQIGEVSADEM
                     FLTAQRHNKMLTWALRAGGFLMMLLGFSMVFKLLSVLGDVVPVLGSIIGAGTGLIALL
                     LSLALSSVTIAAAWIFYRPLLGVALLALTVGLALLIRRKMAAARPD"
     gene            complement(17210..>18215)
                     /locus_tag="PKK36_05010"
     CDS             complement(17210..>18215)
                     /locus_tag="PKK36_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNR93953.1"
                     /translation="TLFDLNVDAIIRMAAGSSDARVLSTPVILTTDNTEAKIIAGEER
                     PIVTATTTSTEGAQTSQYEYKNIGLELTVTPHINPKGFVVMEITQTADNLLDPVQIDG
                     NEVPVISKRELTAEIAVNDRSTIILGGLVNTRKSKGRSKVPVLGDIPLLGTFFRADSR
                     SDTRSELIVLITPYVLLTPEDALEESDRLHRASRASKTPWPKNWSRSPLATPDPKDRE
                     AVLQAEKNAADLKAMIERRKEEIRREGLDTSRAKPADVIEVVPVSEAGDSGETETAPP
                     AEENPAAQIPEEMEENETMIADGMVMFDIPDPDEEEGAAPAPEEPSEEMPEETPPPAE
                     "
BASE COUNT         3497 a         5527 c         5188 g         4003 t
ORIGIN      
        1 ttgagatctg agattggatg tcgttggatg ccgccgtcca gacgggatcc gtctccgtga
       61 acgcatacag cggcgtgccg gtcgcgtacc cgcttttctc tgcatcccaa acaggatccg
      121 tctcgacata aaccggcatg ccggtcgcat agtctgcttt ctgcgcatcc cacactggat
      181 cggtttcggt gtattcgtac agaggtgcgc ctgttgcata attgctgcgt tcggcaaccc
      241 ataccggatc ggtttccgtg aacacataca gcggcgtacc ggttgcgtac ccgctttttt
      301 cagcatccca aacgggatct gtctcaacat ataccggtgt gccggtcgca tagtctgctt
      361 tttgcgcatc ccacactggg tcggtttctc cgtactcata aagaggcgcc cctgtagcat
      421 aattgcttct ctctgccacc catacgggat cggtttcagc ataaaccgga gttccggtgg
      481 catatcctgc ctcgctgtgg tcaccccagc cgtgcgcttc ggaccactcg gaagtgttca
      541 gtgcggagat tgcaaaggcc gcggaggccg cgaagaccgg atcggtttcg gtaatactcc
      601 cgccccctgc agagatttcc tgccacgcac cggaaccgaa ggttgaacgg taaaacacgc
      661 tggttccccc atcaagatac agactgccgc gaaccgcggt gctgttgtct atctcgttgg
      721 tcagggacgc cccaattgct gttcggctga atccatccgc agcgttcgca aaagctccga
      781 tggcaatgtt tgttcccgcc gcattggctt ctctgccaag cgcggcgccc agatatgatc
      841 catttgcccc ggctccaacg gccactccgt catcaggggc aattgcaaaa cttcccagag
      901 ccgcacccga cagcgacccg tttgctctgt atcccattgc ggagccgttt tcggcagctg
      961 cggcggtatg tccaacagct gtgccttcat ccgagccatt tgcgccatat ccaacagctg
     1021 tcccggtgga atacgcattt gcttcacgac ccaccgccgt accgcctccg gttgccgcgg
     1081 catttccgcc cacacgcgtt tcaaggcccg tgcttccgat aaccagtgcc ccgccttcgg
     1141 cgttgacgat cagttctccg gtcatggtgt ctccggcggc atcaacgtaa aaatcatcac
     1201 tttctgtttt ctgatagtag aaattgctgg cagagctcca tatcggatca gcttcaggct
     1261 cgcctcctcc actgccggag aattcctgcc attcgccaga gccaaaagtg gaacgataga
     1321 agacactcgt cccgccatca agatacagac tgccgcgcat gacgaccgtg tcggcctcct
     1381 gcgtattgac cacgtgattg cctatggcga ttctgtcggt gccggcggcg ttcgcctggt
     1441 tgcccagcgc aatgctctcc ttgttcgcaa acgcacgatt cccgaccgcc actccgtaat
     1501 cggaagcgct tgcggtatac cccgcgacca cactgcgaag tgttcctgtg gcggcgtaac
     1561 cgacggctat ggcctcgttg tatgccagcg aattgcgacc aactgccgtg ccaaacaaac
     1621 ttccgttcgc ataacggcca atggccacgc cggaattggc cgcgttggct tccccgccaa
     1681 cggccgcgcc cccgttgtac ccattggcat aatcgcctgc ggcaactccc gagactctgc
     1741 cgacagcccc cctcccgagc gcagctcctc cggaatatgc atttgccccg tgtccgacgg
     1801 caacgcccac actggacccg ttggctccat ttccgacagc cacagattcg ccattgactg
     1861 cagcactgtt aattccgatc gccacgctgc ggtcataggc attcgcatta taacccaggg
     1921 ccgtcccctg atcggcgccg ttggccagcc gtcccaccgc aaccccggct gacgcggcat
     1981 tggcgtcgag tccgatggcc gtcccgctgg ttgttgccac tgcgctgttg ccgatacgaa
     2041 ccgccattcc cgtgctgccg atcaccagcg cacccgcatc ggcgttgata ttcaaggctc
     2101 ccgtcatggt gtcgccagct gtttcaacaa aatcgcctcc tcctccggag actagcgcat
     2161 aggccacgga aacaaggcgc tggcggggga gaagaatcgt tccgtccact tcggcctcaa
     2221 cccagatctc ggtattggtc agggcatcct gaagcgagcc ggacaacgtg ttgtcgccga
     2281 tgaatgtgga atacagccca tccaccgcgg tcacggtgtt ggagtcctcg tattcgagtg
     2341 ttccggcgga agcgtcattg tagaggcgga gcaccagctc gacattgccg ttgaccagca
     2401 tgccgccgtc aaccagccgg ccctggtaat tgatcatgga gggaacctgt gcccgcgcct
     2461 gtggctgccc ggcgaatact gtgagaactg ccgcaaaagc tgcgcactga tgcttcttca
     2521 tctttcttcc tccttgcaaa aaacggccgg gtgccgcatg ggacctgaac tgattaaagc
     2581 ccccgcggca ggccgccgca agaggttttt acccggaatt tccaatcact ggaagaacgg
     2641 ggctgaaaac ttccaatgat tggaagtttt cagccaataa gttccaggga ttggaacttt
     2701 tactggtcgc ccgcgggcgg agtctcagag acgggcgttc ccgccgtctc tttgtcctcc
     2761 tcctcgtcct cgacttcagg ctcgagcggg gtggctgaaa tgaggcggtc gccttcgtcc
     2821 agattgatga cccgcacacc ctgcgtgttg cggctgatga cgcgcagatc actcacgccg
     2881 aaacggatca tctgcccgcc ctgactgatg agcatcagcg catcctgatc cttcaccgag
     2941 agcgccgcca ccagcttgcc gttgcgctca gtggtgcgaa tggtgatgat ccccttgccg
     3001 ccgcggctct gcctgcggta ctcatcgaag ttagtgcgct tgccgtagcc gttttcagtg
     3061 atgctgatga atgtggcgga ggggtccact acagcgatgt tttcgacgat gtcgtctttg
     3121 ctgagggtca tgcccttgac gccgcgggtg gcgcgtccct ggtcgcggag ttcgccctca
     3181 tcgaagcgga tgctcatgcc gttgcgggtg gtcaggatca ggtcctggcc ggcgacggtt
     3241 ttcaccacgc cgatgagttc atcatccgga tcaatgctga tcgctatgat gccgcccgcg
     3301 cgcacgttgc tgtaggcgga aaggttggtt ttcttgacaa tgcccttgcg ggtggccatg
     3361 acgagatgct cggtgtcact gaactcacgc acgcgcagca tggaggccag tttctcatcg
     3421 gttccgacct gaagcagatt gacgatggcc ttgccccggg cggtgcggct gccctccggt
     3481 atgtcataga ccttgatcca gtgcatccgg cccgcctgcg tgaagcagag cagataatca
     3541 tgcgtagtgg ccatgaagac atgttcgaca tagtcctctt ccttggtgtc catgccaacc
     3601 acgcccttcc cgccgcgctt ctgctggcgg taggaactgg tcgggacgcg cttgatatag
     3661 ccggtatggc tgacggtgat cacgcagcgc tcgtcggcga tcaggtcctc gatattgatc
     3721 tcgccggcgt cggccacgat gtcggtgcgc cgcttgtcgc cgtagcgttc cttgatttcc
     3781 agcagctcct ccttcaccac gccgaggatt ttggcgggag aggcaagcag gtcacggaga
     3841 tagctgatca gtttgatgat ctccagatat tcgttctcga ccttgtcgcg ttcaaggccc
     3901 gtgagctgat acaggcgcat ttcgagaatg gcgttggcct gaagttcgct gagtccgaag
     3961 cgggccatca gcttgacgcg ggcatcgtcg cggtctttcg cctcgcggat ggttttgaca
     4021 acggcatcca tgtgatcgat ggcgatctta agaccttcga gaatgtgggc gcgggcttcc
     4081 gccttggcga gatcaaactc cgtgcgcttc gtgatcacat ccacccggtg atcaataaag
     4141 catgtcatga ggtctttgag gttcatcacg cgcggtttgc cgtggtcaat ggccagcatg
     4201 atggcgccga aggttgtttc catctgggtg tgcttgaaaa tggaattcag gacgaccttg
     4261 ggcacggcgc cgcgcttaat ctccaccacc acgcggatgc cttctttccc ggactcgtcg
     4321 cggatgtcgg atatgccctc gatggttttt tcgttcacca gatgggccat tttttcgatc
     4381 agggaagcct tgttgaccgt gtacgggatt tcggtgatga taatgctttc ccgcccgttg
     4441 ttggcggttt caatgccggc gcgcccgcgc accttcagtt tgcctcgacc ggtctcatac
     4501 atctcccgga tggagttcag cccgcagatg acgccgcccg tcgggaaatc cggccctttg
     4561 acgactttac agatttcagc gaccgggatg tcggggttgt cgatgagcag gatcattcca
     4621 tccgcgattt cggccaggtt gtgcggcggg atgttggtgg ccatgccgac cgcgatgccc
     4681 gtgctcccgt tcaccagcag attcgggatg cgcgaaggaa gcaccgccgg ctccatcagg
     4741 ctttcgtcaa aattcggacg catctccacg gtggacttct caatgtcagc cagcatttcc
     4801 tcggcttcgg ccttgagccg gcactcggta tagcggtacg ccgcggcgcg gtccccgtct
     4861 atgctgccga agttcccctg gccgtcgata agaatgcacc gcatggagaa atcctgcgcc
     4921 atgcggacga gcgtgtcata gacggcgctg tcgccgtgcg gatggtagtt gccgatgact
     4981 tctccgacca ccttcgcgca cttgacaaac ggcttgctgt gaatccagcc ccgctccttc
     5041 atggcgaaaa ggatgcggcg gtttccgggc ttgagtccgt cgcgcgcgtc gggcagcgcg
     5101 cggccgatga tgacactcat ggagtagtca atataggccc gctgcatttc atcttcgatg
     5161 ttgatcaaat ggttctgatt gttttccagc atggggggaa ttccggtttg ttcgttgtta
     5221 gacatcgaga ttctgcacat tcagcgcatt ttgttcaatg aattcgcggc ggggcccgac
     5281 atcatcaccc atcagaatcg tgaagatttc atcggcgcgc acgtcatcct cgaggacgac
     5341 cttgaccagc ttgcggttct cgggatgcat cgtggtttcc cagagctgct cggggttcat
     5401 ttcaccgaga cccttgtagc gctggatcac cagcccgcgg cggcccaggt tgcgcacggc
     5461 atccagcaga tcggggagtg aaccaatcgg cgtcctctgg tccttttcat cggccagaaa
     5521 gtaaatcggc tcctcagcgg gctggtactg gtccaggctg aagcccatct tctcaagctt
     5581 cgcgacagcc tttgccagat ccaccgcccc gtagaactcc acccaccgca ggccgctctg
     5641 gtcgggcttc gattcgacat tctcgccgtt tgtgaaaatc tcgagctgaa ggcccagctt
     5701 cttttccgcc tcctcgcgga gcttgcggag ctcgcgctcg tcataagcat aaaagtgctc
     5761 ggtttcgctg gtgtccctgt aggtaatggc cacccggtac tgggggaaac gccccgtttt
     5821 gggatcgcgg cgggtcagga actcactaaa cacggcaccc cgccgctgaa tgcgattgcc
     5881 cagagtttca atttccgaaa gcagttcaag cagtcgaatc aaggctttat caggcagaac
     5941 ttcttcgccg gccggattca gcagtttcac gtcatccgaa ccgagatcca gcaggatctt
     6001 cgtgagcgct tcatcactgt ccaggtactg ctcgcgtttc ttgcgcttga tcttgtagag
     6061 cggcggctgg gcgatataaa tgtacccctt ctccaccagc tggggcatct gacggtaaaa
     6121 gaatgtcagc agaagcgtgc ggatgtgggc accgtccaca tcggcatcgg tcatgataat
     6181 gattttgtga tagcgggctt tggcgatgtc gaaatcatcc tcgccgaccc cggtcccgat
     6241 ggcggtgatc aatgtgcgga tttcctcgtt gctgaggatt ttgtccagac gggccttttc
     6301 cacattaagc accttgccgc gcagcggcag gatggcctgg aagcggcggt cgcggccctg
     6361 cttggccgag cctccggcgc tgtctccctc cacgatgaat atctccgtca gcgccggatc
     6421 gcgttccgaa cagtccgcca gcttgcccgg gagagaactg ctctcgaggg cgcccttgcg
     6481 gcgggtcagg tcgcgggcct tccgcgcggc ttcgcgcgcg cgcgcggcca caacggcctt
     6541 gtcaatgatc cggcgcgcca cagagggatg ctcctcgaag aaggtggaca gttcctcgtt
     6601 tacgatggac gccacaatcc cctccacctc gctgttgccc agcttggtct tggtctgccc
     6661 ctcgaactgg ggctggggaa gcttcacgct gatgacggcc gtgagcccct cgcggatgtc
     6721 gtcgccgctc atgccggcct cgttcttggt gagcttgttg gtctttgcgt actggttgac
     6781 cgtgcgggtc agcgcgctgc ggaaaccgct cagatgcgtg ccgccctcga tggtgtttat
     6841 gttgttggca aaacagtaga tcgtttcctt gtaggtgtcg cagtactgca tcgcgatctc
     6901 ggcctggatg ccgtcctttt cgcgctcaaa gaaaatcggc ttgggatgca gcgggttctt
     6961 ggcccggttg agatgtgtaa cgaactcccg gatgccgccg ctgtactgga aaacctcctc
     7021 gcgggcgggc tcctcctggc tcagcttgat caggattccc ttgttcagga acgccagttc
     7081 gcgcaggcgc accgccaggg tgtcccagtt gaactcgctg gtgctgaata tctgcggatc
     7141 gggcttgaag gtcaccttgg tccccgtgcc cttggtggtg ccgatggtct ccagcttcgt
     7201 caccggaacg ccgcgctcat aacgctggtg atacaccagg ttgttgcggc ggacttccac
     7261 ctccagccat tcgctgaggg cattcacgca ggaaacgccg accccatgca ggccgccgga
     7321 caccttgtag gactcgttgt caaactttcc accggcatgc agggtggtga gcaccacctc
     7381 cacggcgggc tttttctcgg tggcatgcat gtccaccggg atgccccggc cgtcgtcgtt
     7441 gaccgtgcag gagccgtcgg cattcacccg caccagaata ttgctgcagt agcccgcaag
     7501 ggcctcgtca atgctgttgt ccacgacttc atacacgcag tggtgaaatc cgcgcacgct
     7561 ggtgtcgccg atatacatgg ccgggcgctt gcgcacggcg gcaattcctt caagaacagt
     7621 gatcgtggtg gcgtcgtatt tcgcgccagc tttctgtttc gcgtcttcag tttctttgga
     7681 cataattctt ctcttattaa cctgttgaaa gggttgaaat ctgacgtctg gaagcccctt
     7741 ttatccccag gcaaaacgtc atacattcag caaaaaaaga ttaccccgaa aggcaggcaa
     7801 aaatcaatga aaatcggcga gaattcggcg atttttcagg gccggatgga cggctctgcg
     7861 gggggcaaaa aggggctgaa aacaggcgtt tttcattcag ccggaaggcg gcggccggag
     7921 gcgtctgtta tccacgcggt gagcgtgatg tccagcgcgc aggaatcctc ctggctggaa
     7981 ccgccaagca ggaagcggga gtagaattcc tcgtccttct caagccctcc gatgcggacc
     8041 ggaacaccgt tcagggcagt cacttcaagg cttgcccggg tgaacttgac acagcagggt
     8101 ttgtcgtaat ccagacccga gagttccgga gtcaggacca gtttgatttc cggtccgtca
     8161 ccaataatgg tcggctcgac ggtcaggaaa gcccccacct cgttccagtg catgcgctca
     8221 tgaatggcgg cccagcgcca gccgtaatcc acgaaccatt ccatcatcgg cacactcgtg
     8281 cccacctgaa tgcgcgcagc ggatccgctg gccgccatga tggactgcac cgtttcgccg
     8341 ccggtctccg tggtcatatg atggacttgc ggcttaataa caatctttga acccgtcttg
     8401 ctgatctctc ccccggcttc gacagaggcc ccggaatcct ttgtgcggcc gacggttgtg
     8461 aagcggacct cgatgaaaac atttttccca atgactggaa ttttcgagaa catctctgcg
     8521 agcgacttgt gctgctccgc ctccgcgata accagcaaac gattgccctt cccgtccagc
     8581 accacagcgc cctccggccc cacaatcgcc tttgcagtct cggtcagggc cgccggatca
     8641 ttattgccca gcggataggc ctcgaaggat ttctgggcaa gagccacaga gcagacagtg
     8701 aagaaggaaa tggcaagtaa aacttgaatg tgctgtttca tgcccaaaga ctacacgcga
     8761 acccccagca atgcaacaga gaggatgctg gagggacggg ctctgttcat ccgacgaagc
     8821 ctctgggcga agtcgggttc cgtccgtttc ctgttgcgcc cccggcgggc ttgtcccgcc
     8881 ggggcgaaaa gtcgagcgag cgaggccgga cccgcagagc cttcctcacc tgcgacacaa
     8941 agtcgcgggg gtcggcgccc gtccacatcc cattctttat ccggccggaa cggaggccgg
     9001 ccctccagtc gccatttatg atgcttgagc acgggttcga tcctcaatcc gcaatttttt
     9061 tcgaatttta aagaattttg gccaatttta acgaaattgt gctgtttttg ggccattttt
     9121 agccattttt cgcgtttttc gggccttttg gtgccctttt tcggcaactc ataaattggc
     9181 cacgaagaga cacaaacggc gcaagatcga atcagggatc ttcttttacg attcgtgccg
     9241 ccggaatttc agtttattcc tcgaaggcgg tgacatcatc aatggaggcg gagtccgtca
     9301 ggagcatgac gaggcggtcg agtccgagcg cgatgccgcc ggagggcggg atgccgattt
     9361 caagggctct gataaaatcc ccatcttccg gataagccgc cttgttctcc aactcgcgga
     9421 gcatgcggct gtgagcgaag cgctgtttct gctcggcgga atcggtcagc tcagaatagg
     9481 cgttcgccag ttccagcccc ccaatgtaaa gctcccagcg ctccgccacg cgcggatctt
     9541 tggcggaagg ccttgaaagc gccgcgcgct caaccggata gtctgttaat accgtcggca
     9601 catcgcgcgg aagcgatggt tcaacttttg tcaccaagtc ctcgtcaaag cggtcgggat
     9661 caaatttttt cagcggatcc cagcgggccc agcgcaggaa agcctcctga acggtcaaat
     9721 gctcccagac cggcaccagt tcaatggcgc ggccctgata cgtcagcgag gtcgtgttca
     9781 gcaccgcgcg cgcagcagaa acgatcatgg ccttggtgtc gagcaggata tcggaatagt
     9841 cggcatgggc gcggtaccat tctagcatgg cgaattccgg acggtgccgg tcgccccttt
     9901 caccggcccg aaagcagggg ccgatctgga aatcttttca taacccgccg caagcatccg
     9961 cttcatgtga agctcgggcg atgtccgcaa aaaacagtcg ccggagggtt cggcatcaat
    10021 gtattcctcg ggggcgggcg cgcggatacg gactggagta tccacctcaa ggaagccgtg
    10081 cctgtggaag aaatcgcgca cagcccgaag gatcgccgcc cgcttctcga gcgccgggcg
    10141 aacctcctgc aggcggttca ggtcggggct gtagggcaat gattccaatg tcagctcacg
    10201 cggccggcgt aggacccgtc gcgcgtgtca atttttatgc ggtcgccctc attgacaaaa
    10261 agaggaacct ggatttcgta gccgttgtcc agcgtcgccg gtttcaagac gtttgtggcc
    10321 gtatcaccac gggccccggg ctcggtgtgc gccaccgtct tctcgatgaa gttcgggagc
    10381 atgatttcaa tgggccggtc attgtgaaac aggatgtcca cctccatgtc ctcaatcagg
    10441 aaatacttct tgtcgccgac cacttccgcg gcgatgtgga cctgctcata ggtttcggca
    10501 tccatgaaga catagtggtc gccttcctgg aacgagtaga tcatgtcccg ctcttcaagg
    10561 ttgcacttgt cgatccggtc cacggagcga taggttttgt caaatgtgct tccggtgacc
    10621 atgctcttga gcttgcagcg gtagagggcc tggcccttgc cgggcttcac gaagttgaac
    10681 tccgtgatca tgtagggctc gccgtctatt tcaaccttaa gcccttttct caaatcggat
    10741 gcgttgtaca tgggtcttct ctcctgtgcc gtatataaat taaggggcga taatatgccg
    10801 acggcagagc ggtctgccaa gcggaaactg gggtcagcct ttcttgtcgg ggtcggtcag
    10861 atagcgctcg tccggatagg tgcagggcag cggccggccc atgtactttt cgatttcagg
    10921 gatatagaaa gaatcctctt cgtccgcaaa actgatcgaa acgccggatt tcccggcccg
    10981 gccggtgcgg ccgatgcggt gcacataagc ttcggcctca cgcgggatgc agtagttgat
    11041 cacgtggctg acatcctcga tgtgaagacc gcgccccgcc acatccgtgg ccaccagaat
    11101 gcggtacttg ccctccttga actcgttgag catgcgcgtg cggacattct gcgccagcgc
    11161 accggagagc agcgcgcagg ggaaatgcct cctgtttaac cggtcggaaa gccgctgcgc
    11221 ctcgtcgcgc cggttggtga agacaatcac gcgctgcgga ttgtccgcct tcaggatgtt
    11281 gtaaagaaga ttgaacttct cacttgagcg cacgatatag acaatctgat tcaccgtgtc
    11341 cacggccacc tggtcgggtt cgacctcgat ggtcacggcg tcacgcgtcc actgggctga
    11401 aaggcgaagc acctcgggcg tcagggtcgc gctgaacagc atagtcatgc gctcgtggcg
    11461 cgggggggta ctgttgatga tttttcggac atccgggatg aaccccatat ccagcatgcg
    11521 gtcggcctcg tcaatcacca tggtctccac atggccgaga tggattttgt gctggttctt
    11581 gaaatccagc agccggcccg gggtggccgc aagaatgtct acgggccctt tttcaagctg
    11641 cagggcctgg ttgcggctgc tgtcgccgcc gtaaacagcc acggagcgga gcccggtgta
    11701 tttgcccagc gcctcggcat cccgctgaat ctggagcacc agttcgcggg tgggcgcaat
    11761 caccagacat cgtggagtgc ccagccgacg ctgatcggtg atcggcttgc gaagcatatg
    11821 cgtgaaaacg gaaagcagga atgccgcagt cttgccggtt ccagtggggg cgcggccgga
    11881 cacatcactg cccttcagcg catgaggcag aatttgctgc tgaatgggag tgcagtactg
    11941 aaatttcagg tcataaagag cccgcatcag ctcagggggc aggccgaggt catgaaaccg
    12001 cgttttgccc tcctgcggag gaacatcata aacggaaata tcccagggtt tttcgtcgcg
    12061 cgccggcggc ggcacgacgg ccgggcgggg ttcatcgtga cgaaggttct tcccctccgt
    12121 cgcgggcgcg ccttccggct gccgcctgcg gcgcggcctg cggcggcgtt ttttggctgc
    12181 ggctggatga ggagtgtcaa cctggacatc cggatgcggc ccgggcgcct ctttagccgg
    12241 ggcagcggac ttttggggcg gcgcttcgcg gcgcttatgc ctggaaggcc cccggtccgc
    12301 gcgctcttcg cgacggcggt gcatttgcgg aggatgcgcg ccctcaacag gcgctgtttc
    12361 ggccagatgt tctatctgcc cgtctttgct tttcttaaaa aaatctacca tataccttgc
    12421 ttttctgaac cttcaggaat gctcattcaa tcttgaggga aactggaaat tcgtgcgcaa
    12481 cgtaagtcat caaagccccg aatgcaacaa aaaacgatca gaataccgga aaacggagtc
    12541 aatctatcct gtatctggcc gggattttat gaccggcttc gggaggcacg gaacgtacga
    12601 tcatgtgcag atactcgttt ctgcccggtt tcttttctgt caggttgacg acctgcggga
    12661 ccctcaatgt ttccccgctc ggggcatacc atatttccac aaccggccgg cgaatgtctg
    12721 caggattggg tgcaattaca attccgacgc tgccgtcgtc gaggaaaacc agcgagcccg
    12781 gcggataaat gccgattgcc ctgaagaaga ctcccaggag cgaaggatga aacagccccc
    12841 cgccctcctt gatcatttcc ttgaaggcga gttccggcgc aagtccgccc cggtagggac
    12901 gacggcttcg gagagcatcg taaacatcgg agatggatac gatcatgctg gccaggcaaa
    12961 gttcggttcc aaacactttg cgaggatagc ccctgccatc atagcgaagg tggtgctcaa
    13021 atgagactgt gggcaccagc gggctcaaat ccggtgtctg cagcaaaagc tttgcgccgt
    13081 ccagcggatg ctgactgacc ttctcgtatt cggattcact caacttcccc tttttccgca
    13141 ggatgtcagc ggcgagagcg atttttccgg agtcgtgaag aaaccccgcc agacccaggt
    13201 cggccagtgc ggatttcggc agtccgagcg cctcgccctg cgccagcgaa agcacggcaa
    13261 cgttgatgga gtgaacaaat gaatattcat catgcttctt cagggatgtg gcgatcagaa
    13321 agggcgcctt gttgcccaga aagcctttca ttaccttctg gatgaaagtg ttcgctgccc
    13381 gggtattgac aggcaggcca tcgtggagag cccggcctat gtcccccagc agccggggcc
    13441 cttcccgata ggtttcttcc acagtgccgg cagacagtac ggacccagac ggagaggctt
    13501 caagccctat accactgatg gtgatgctgc ctaattcgcc ggcggccgtc gtcatctcgc
    13561 ccgatcggca ggtcagcagt tcgataaaag acctgatgtc agccagaggg acctctccca
    13621 tgaaggtcag tttctcaact ttgtgctcct cgagcgcggc gatcagactg gacatatgac
    13681 gcgtcaggtc atagaggggt ttatttccaa acgccagttc cccgccgatc acgccgatga
    13741 cgatttccgt gcgccccgcc atggcttcgt gcagagaggc gcgtacccta accgcggcag
    13801 cctcagtcag cgcgtgtttc ggtccgtaaa atcggctgtc ctgaatggct gcctgcagat
    13861 caagaagaaa cccttcaacc ccccggcttt tttgttcagc tttcatgccg ccggccccct
    13921 ccttgcgccc gtcagcggca taacccgccg gcatgcgcgc tgaacattcc ggttcccttc
    13981 ctgaatggtt ttctgaagaa cagcggccgc tgcggaagta tggatccggc agaggctttc
    14041 cacaaccgcc tcaacaatgc cgagacggcc gggcatgggc agtgtgtgat gcttgagcag
    14101 gccggaaaga aacggaactg atgccgcaat ctttccctgg ccgcagatat cgatcagttc
    14161 cagcagaatt cgatgtgaaa atactccccc ccgaagaaac ctgaaaagac ggccgattac
    14221 agtctggtca ccgcttcccg ccagcacgca cagataaacc cttcgaactg ccggatcttt
    14281 ctcgcgaaca gccttcttag caagatcagc cgcctgttcg tcggagagtt tcatggcggc
    14341 cagagccgcg catatttcgt ttccggccgg accgcgctcg cggttgatcg cgagattcag
    14401 aagcgccttc ccgccatccg cataagtttc ggacagcaag tgcgcaatct cctcaatctg
    14461 cgatggagaa gagccgggaa tcagtgccgc gagggcttcc gaccccgcaa ggggcccaag
    14521 gcgatacagg cttgaaacgg cttcccgggc gatggccggg tcgactccgg gacatgtctt
    14581 ctcccagaaa accttcagcg catcgcgtgt catttcgggc cgcgtatttt tgctgcagcg
    14641 gggaagttca tccagcagcc gtccgctgaa ctctctgaag gtgcggggat tctcctcaaa
    14701 ccagaggatg tttaaaagca gccggacctt ttcatcctga gcgcggtttt tttcgcacat
    14761 cagctgaaag tttgcgacaa gggcggcaac ggccgcatca gtctgaattg tctgccgctc
    14821 cttctccgcc agcgtattca tcgtaagctg atagacctgc gacatgaatt cgtttctggc
    14881 ggctgtcttg agcgcatctt ccagcgctcc ctgaactgtg gccagacgat catgatccag
    14941 gccggacagt ttgcctgcaa cggattgagc cagatccggc gacgcacccg ccctgtccga
    15001 caatcgcaga aacaggctca tcaggctgct gtccaccttc ccctcgcgcg tgatgtgcga
    15061 cgatatcatt tcagacagcg ccgcgtcagg aaccgccggc agcgcggcct tctggagatc
    15121 atccactccc tcttctgcgg cttgttcaga gaacagggcg tcgagggcct ccggattcag
    15181 ccccgcgaga acggtcgaaa ggtgcctaag ggtcctggcg gctttttcgg ggggcaagtc
    15241 tttgcagaac cggaccaccc tgcccatcac gcgccagaga ttttttgccg cctccccccg
    15301 gtgaccgccg ggggatgtga atatctcatt gatcgcagcc gagaacttcc tcccgccggc
    15361 aagagcagcg catatttctt cgatcacggc ggcggaagga aatcctgccc tcgccctctt
    15421 gtctgcatcg cagagaagct gaacaatatc tatatcgcgg ccccctcctg ccgcatccac
    15481 gctccgcgtc aaaagagttg aataatctat ctgctttgcg gaaagatgcg gacagcgcct
    15541 gagtctggac gaaacgccgc cggaccgggc aatgatctcg ggcgggtcct tgatggccgc
    15601 cagcagcgac gacacctcgg acgccacgac gcccggacgt attgtcaggg ccagcagccc
    15661 cctctgatga aaatgcctcg ccaggccggc gagaaccgga ttcccttcct tgaaaggctt
    15721 ccctccaacc agaacagcgc tccgcgtaaa cgccatgtcc agcttccctt cggaacccag
    15781 ccatccgtcg agagctgctt tgagagactt gagcgaatcc gcaagcatgg gatgccctgc
    15841 cggatacatc gcactgttcc ggatggcggt attgatgaac cgcaccagtt ccatgaatgc
    15901 cgcagtcttg tcgctgcctg aattctcctc gaccatggct ccccgcacct tgtgccccaa
    15961 acaagtcccc tctatgcaga aagcttacca ctcaggcgcc tgatgctcca gacaaaattc
    16021 cgggatttaa agagcttcag tccggccttg cggcggccat tttcctgcgg atcagcagag
    16081 ccagcccgac ggtcagcgcg agcagcgcaa caccaagaag ggggcggtag aatatccatg
    16141 cggcggcaat ggtcacagag gaaagcgcca gtgacagcag gagggcaatg agacctgttc
    16201 cggccccgat aatgcttccg agaacgggga ccacatcccc cagcacggaa aggagcttga
    16261 agaccatgct gaacccgagc agcatcataa gaaatccgcc ggcgcgcagg gcccaggtga
    16321 gcattttgtt atgccgctgc gccgtcagga acatctcgtc cgcgctgact tccccgatct
    16381 ggagaagctg aatcgttccc ccgtttttgg tgacataggg ctcaaacgac accccctgct
    16441 ggccggaaac caggctgatc acctgaggct ccacagcgga aaacgcaacc cgaaggtctc
    16501 cgatctgcgg agcggccgga tcggccccaa tatagaatcc tccgggctgg cggcggatcg
    16561 ctgcggaatc tgccgggccc tcaccctccg ccggattgac cgcagaatag gccctgatct
    16621 tcgcaatcag cgatgtgctt aaagaaaacg ccccgactgt cacactttga gcgaccagtt
    16681 catatgtctg gtatgtcatc gccccgggat tgtcatgacc ggactgccgg aacttcccgg
    16741 aatcgatcag cgattcgctc cagaccttct tgtaggtgta tgtcgtgacg gtctctgttc
    16801 cgccgcccat tttcttcttt gtgcggcttt cggcgtcttc atcccactga tacatttcca
    16861 cgctgcgacg gagctttatg gctttgcggg aaacaccgaa aacgggatcg gtcagcatct
    16921 cttctgtctg cgccaggccg gtcatatgaa ccagctttcc tgaattgttc tgatccaccg
    16981 aatcacagga cacggaaatg accgctccgg cgccctcctg aagcgtttta taacgtttga
    17041 cagcccggcc ctcgttccag aaaaggagcg ggaatgcgac aataatcagc agcagtcccc
    17101 ccaggatgcc ctttacggct ccgccaaggc ggctgaacca ggactgtttg gaaacttcag
    17161 tataggtgtc cgcgcacatt ttcaacctcc ttcagacaac gggccggggt tactccgccg
    17221 gcggcggggt ttcttccggc atttcttcag acggctcttc gggggccggc gctgcgcctt
    17281 cctcctcatc cggatccggg atatcgaaca tcaccatgcc gtcggcgatc atcgtctcat
    17341 tctcttccat ctcttctgga atctgtgcgg cgggattttc ctccgcaggc ggcgcggttt
    17401 ccgtttcgcc agaatcgccg gcctcgctga ccggaaccac ctcgatcaca tcagcaggtt
    17461 tggcccggct ggtgtccagc ccttcgcggc ggatttcctc cttgcgccgc tcaatcatgg
    17521 ccttcaggtc ggcggcattc ttctcggcct ggagaacagc ctcgcggtct ttcggatcgg
    17581 gagtggccag cgggctccgc gaccagtttt tcggccaggg cgtcttgctc gcgcgcgatg
    17641 cgcggtgaag ccggtcgctc tcctccaggg cgtcctccgg cgtcagcagc acatacggcg
    17701 tgatcagcac aatgagttct gaacgcgtgt ccgaacggct gtccgcgcgg aagaaagtgc
    17761 ccagcagcgg gatgtcgccc agcaccggca ccttggagcg ccccttgctt ttgcgtgtat
    17821 tgaccaggcc gcccaggatg atggtcgagc ggtcgttgac ggcaatctct gccgtcagct
    17881 cgcgcttgct gatcaccggc acctcgttgc cgtcaatctg cacgggatcc agcaggttgt
    17941 ccgccgtctg cgtgatttcc atcacgacaa accccttcgg attgatgtgc ggagtgaccg
    18001 tcagctcaag tccgatgttt ttgtattcat actgtgacgt ctgcgcgccc tcggtgctgg
    18061 tggtggtcgc ggtcacaatc ggccgctcct cgccggcaat aatcttggcc tcggtattgt
    18121 cggtggtcag gatcaccggt gtggagagca cacgggcgtc tgacgatccg gccgccatgc
    18181 ggatgatcgc gtccacattc aggtcgaaga gcgtc
//
LOCUS       DAOQWR010000042        18117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3481_length_18117_cov_5.828590, whole genome shotgun sequence.
ACCESSION   DAOQWR010000042 DAOQWR010000000
VERSION     DAOQWR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_18117_cov_5.828590"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4415
                     /locus_tag="PKK36_05015"
     CDS             <1..4415
                     /locus_tag="PKK36_05015"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNR93954.1"
                     /translation="TDTNRLDIVDTNFLMRIGSVSFYVNDATNDEQDVWCLAPGDDVN
                     TGLDPTVPMLTIQALLDTYDIAPGDFIFVDAGYYSLEDDLIINALDSGVATSRVQITG
                     APHRLTVFDRGDTTNETTYGIVFNSATCVTISNIVVQNADVGVDFYRSLSCALSDSLI
                     RDCGQSGVRFFQTKSNLLQRTVITRSGDSGVQDQLSTYNYLDGCVVWASGANAIQGVN
                     SLLCVSNSVLHASGSDRLCYQNSTNAVFLSDYNNLFVENGAFYGKSPSPVGLIGEGMP
                     QWILYTTQDVHSLSFDPMFADPDNDDFHPMSPEGRYVTGTFVQVDAAFSPLIDAGNPG
                     TIVTNETDPNGGRINIGAFGNTAQASRSRTNAWVLAVYASAGGRVAGGAPVLLTWLQG
                     NLDATNRVTLEYSWDNGTTWTNIVSGIPAGDGQYLWDVDEEESGSPKYPASPTARWRV
                     FVEADPSVVDDTDVFFSLKGPFTFYINDLNISNDVYTGAAGNDANLGILSNAPKLTLT
                     SLIQQWDIEGGDTIVLDTGLYIISNEVVITSADAGKAGSPVLLMGSTNAGGALLSRST
                     NNTSLLRINADHMDVAGLSVRGGFLRANGNNLNLSDLNLSTGTVEITSRSSTVTRVTQ
                     VGSYFRLQGSADMVLQRLNMRNARLETKGSSDILVENALIYGTSTNAVLVDESQRFTM
                     RNCTLSVNGNQFLQSGSSLSEIENNIMVANGASRFCILQLGGALDSDYNTLVTRNGAW
                     FGNAEGQWEKLIYWQDESGLDVHSLPHEPLFVNEGGGDYHLQSYVGSWNGTGWSTNAN
                     HSPAIDAGNPSTVYTNEPYPRGPAVNMGAYGGTEEASKSLLDPWLLALTVSDGGVLSG
                     TNTLYWLSGNIIPTNTLRLDYSLDGGVTWITFAPGLDSTLNEYAWDTTANITNSIETL
                     WRVVVEANTNVFDETDEPFSVRNAPIYFYVNDTNTAGDVFCGAPGSALNDGLTTNTPK
                     LTIQGILDAYDLEGYDTVFVDAGYYKLGTETRMIWSDAGKADIGNLLIQGAGTGTVIA
                     GWSRAPAATALRIYGNYIDLRDFTISDAGVGILMDSNHYVTAENLLMISNALGINLQS
                     GANYDIRNSLFLYNTNSAVQMDRVTDASVENNTFYGNAPAAISLMSSVNSVLRNNIFG
                     LTAAEAYAYGGTSNDVANNVTMDYNVYYFSEPGYIYSTFNTLLPWQLNYGHDYRSAIT
                     NPLFAAVDNRDFHLQSAAGRYLPGTGFVTTDSETSWAIDKGDPWADYSLEPDENGARI
                     NIGRYGGTEEASKGSTNVVVYARSLNDPVFITDTNSIWPLIWTALNVPTGTLVTVQYS
                     GDGGVTWEDIATGLNVYEEYIIWQPTFEDNSYSALWRVVDSTNAPVYVDTNDTEAGLF
                     FGTFEISEANNMEGTNMHYIIWRGAWNEAYQVEYSYGFTQFGDIIWSNCVDGVGSNQA
                     AYRFTTSGGNFRYEDIESPNQSNRLYRVKWIQSP"
     gene            4481..5059
                     /gene="rsmD"
                     /locus_tag="PKK36_05020"
     CDS             4481..5059
                     /gene="rsmD"
                     /locus_tag="PKK36_05020"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNR93955.1"
                     /translation="MRIISGQLGGRTIRMPRGDVRPTQDKVREAIFSSLAPLIPGARV
                     LDLFAGSGAMGLEAWSRGAAAVCWVEQNRSVFRTLQQNVRELCGDSPQAHCVCADAFS
                     FLRNGGGVKYDIVFCDPPYGKTERGRLEQMLNVLGGSPSLPPGALLVYEHGAAEAPAD
                     SPLWELKRSRRYGKTGVVIYQKLSERRDCGEH"
     gene            5049..5537
                     /gene="coaD"
                     /locus_tag="PKK36_05025"
     CDS             5049..5537
                     /gene="coaD"
                     /locus_tag="PKK36_05025"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNR93956.1"
                     /translation="MSTKAIYAGTFDPLTLGHMDLIRRASGIFDELILAVAGSTRKQT
                     LFSLEERVAMATEVVTPFKNVTVEGFNGLLVDYARSRKIHVVVRGLRAFSDFEFEFQM
                     ALMNRSMASDVETMFLMPKQDYSYVSSSSVREVAELGGDVTAFVPPAVKRALDLKLKN
                     RV"
     gene            5544..6005
                     /locus_tag="PKK36_05030"
     CDS             5544..6005
                     /locus_tag="PKK36_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNR93957.1"
                     /translation="MRIETARLTPAGSLFKGEHPASVLELEHEALLHAEEPVVYELTV
                     RLVSHEVLVQGRISTKIEVQCSRCGEFFSTLVEDSSFLCTYEIEPGQDSLDITEDIRE
                     AILLLIPAFPVCREDCRGVCMQCGRNLNEGPCSCKKKVLNDPWEKLNRLDL"
     gene            6052..6237
                     /gene="rpmF"
                     /locus_tag="PKK36_05035"
     CDS             6052..6237
                     /gene="rpmF"
                     /locus_tag="PKK36_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011667786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNR93958.1"
                     /translation="MAVPKRKKSKSKKRMRKRSLSGEILAARPCPQCGAPVQSHRACP
                     SCGTYKGRQVVSVTADE"
     gene            6243..7262
                     /gene="plsX"
                     /locus_tag="PKK36_05040"
     CDS             6243..7262
                     /gene="plsX"
                     /locus_tag="PKK36_05040"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNR93959.1"
                     /translation="MRIAVDAMGGDFAPAQNVEGVLEALETLPQIEHLFLVGIEEAII
                     KALPPGRSFPSKVEIVPASQVVEMHESPAVAVRRKKDSSIGRAIDLVKQGKADAVFSA
                     GNTGAAVAACTLKLRTLEGVDRPAIATVMPTTGDKPFVLIDAGANTDCEPELLYQFAV
                     MGSIYAREIIGISNPRVGLLSIGDEDAKGNEETKAAFAMLEKSSLNFIGNVEGHDLYS
                     GEVDVVACDGFVGNVVLKTSESAAHSISRWLKIELTRNYLRMLGAFLSQGAFKTLKKK
                     LDPAAYGGAPLLGTNGVCIIGHGSSSPHAVRNGIRVACESVNHKINHHIVSALADSAS
                     GEKSE"
     gene            7255..8277
                     /locus_tag="PKK36_05045"
     CDS             7255..8277
                     /locus_tag="PKK36_05045"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNR93960.1"
                     /translation="MSKKEKFSDAGRLRTVSIAGTGSYMPERIMTNAELETMVETSAE
                     WIVTRTGIAERRLAGESETTSDMGAEAARRALADAGVSPEEVDLIVLATITPDLPFPN
                     TACLVQDKIGAKNAACFSLEAACSGFVFAMETARQFVATGAAKTALVVAAEKMSSIID
                     WTDRETCVLFGDGAGACVLKAAEGDKGLVHAVLGSNGALAELLMVPGGGSRLPFSEEV
                     LRERNHYLKMEGREVFKHAVKSMTAIAQQVCAEKGLTTKDVACIVPHQANIRIIEAIG
                     QRLGEPKEKFFINLDRYGNTSGASVIVALDEAVRSGVIKRGDLVMLLVFGGGFTWGAS
                     LLEWSK"
     gene            8274..9209
                     /gene="fabD"
                     /locus_tag="PKK36_05050"
     CDS             8274..9209
                     /gene="fabD"
                     /locus_tag="PKK36_05050"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017437119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNR93961.1"
                     /translation="MMKTALIFPGQGAQAVGMGRDLAEAFPACKELFDRAGAVLGCNL
                     AQLCFEGPEELLTRSDNAQPAIFTASIACLEALKLEYPGFSFDVTAGLSSGEWAALHA
                     AGVLSFEDAIRILVARGRFMIEACHQQPGAMLSVMNLDLEILRKISAEAGIEIANLNS
                     PGQTVLSGRVEGIERAEILAKEAGAKRAIRLNVSGAFHSSLMNSAADRLAEVLAGISF
                     RTPSVPVVSNVTAEPHGTLETIPALMVRQVNSSVRWVESVEWMKAHGVSRYIECGPGK
                     VLSGLVKRIDKEAICLNILDLESLRSSQAALQGIG"
     gene            9209..9949
                     /gene="fabG"
                     /locus_tag="PKK36_05055"
     CDS             9209..9949
                     /gene="fabG"
                     /locus_tag="PKK36_05055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNR93962.1"
                     /translation="MDLNGKVAIVTGAARGIGQAVAVRLASAGADIALCDVQAGWLDE
                     TAGRIAELGRRAEKYAVDVSSSADVEAAISQIHQDFGHIDVLVNNAGITKDGLLARMS
                     EQDWDAVLNVNLKGAFLFCKAASRIMMKQRTGSIVNMASIIGLIGNAGQCNYAASKGG
                     LISLTKSVAKELAGRNVRANAVAPGFIETKMTEKLSPDVQQKMLEAIPMKRFGKPEDV
                     ANVVLFLASDASAYMTGQTLTVSGGMVM"
     gene            10045..10284
                     /gene="acpP"
                     /locus_tag="PKK36_05060"
     CDS             10045..10284
                     /gene="acpP"
                     /locus_tag="PKK36_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNR93963.1"
                     /translation="MALEDKVKEIIVEQLGVNADQVTPEASFIEDLGADSLDTVELVM
                     AFEEEFGAEIPDEDAEKLTSVGAVINYLKEKGLGA"
     gene            10383..11621
                     /gene="fabF"
                     /locus_tag="PKK36_05065"
     CDS             10383..11621
                     /gene="fabF"
                     /locus_tag="PKK36_05065"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNR93964.1"
                     /translation="MTRKVAVTGLGIVSPLGCSVDTVWNRLMNGESGIGPITYFDTTL
                     FDAKIGGQVKEFDVDAFIPKKEQRRMDPFCHYGIAAAKMAVSDAGLEMEKEDPWRVGV
                     MVSSGIGGLQVVEVQCNNFLQRGPSRFSPFMIPQMITNILSGRIAIEHGMKGPNFCVV
                     SACASATHSIGESLRIIQSGDADVMLTGGAEAPVCVLSVGGFCAMRALSTRNDDPTRA
                     SRPFDRDRDGFVCAEGAGVLVLEEMEHARARGAKIYCELAGYGRTCDAYHITAPDEGA
                     EAAAKGISLAVADAGMNPDQVEYINAHGTSTPLNDKGETLAIKKALGETAARKTMVGS
                     TKSMTGHLLGAAGAVEAIFCSMALKKGQIPATINLENPDPACDLDYVPNTAREVPIKT
                     ALSNSLGFGGHNATVAFKAV"
     gene            11783..12184
                     /locus_tag="PKK36_05070"
     CDS             11783..12184
                     /locus_tag="PKK36_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNR93965.1"
                     /translation="MDNTTAQSVIDAQVEITGTIKSGGSIKIDGKLKGDLQCEGDATI
                     GKSAVITGNLTVNSVAVAGAVTGNIVAKDRIQMQSSARVMGDIKAKRLHVEDGVTFVG
                     KSEVNPSGGPVSSTPKPEAEESRSGMFSGKK"
     gene            12218..12928
                     /locus_tag="PKK36_05075"
     CDS             12218..12928
                     /locus_tag="PKK36_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNR93966.1"
                     /translation="MAPRKAGEVDGFMALKAAQRAVKKAPAAPVPPAPPSVAKDSGGA
                     QKKIGSHIGKTTMPTRHEVVCYECGFFFQMTGRPKSTFCPKCKANLTFSDHSIETVWS
                     ESLKTAGSILIGPAGVVTAGDFIATDIIVEGRVEDGALMASRFLEVRAGATVSEQAMK
                     SRDLRIAAGASVKLTSAAVYRNVDIAGRLRARLKASGTVNISASGCFEGELTGAHLTV
                     EEGGGLRARLHVEADVKK"
     gene            complement(12936..13322)
                     /locus_tag="PKK36_05080"
     CDS             complement(12936..13322)
                     /locus_tag="PKK36_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93967.1"
                     /translation="MKKAAYLLAAVSAIALIAGCARRAPEQELSPEEKAFAELSQKNH
                     EVFSTTNEFPLRANAEELLRMNRYKPDGYFWRGVAATGEGNFIDAEKDLEIAVMLEPA
                     FPMAREYLQWVREMKSLVNPGGPPAP"
     gene            complement(13389..14540)
                     /gene="chrA"
                     /locus_tag="PKK36_05085"
     CDS             complement(13389..14540)
                     /gene="chrA"
                     /locus_tag="PKK36_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNR93968.1"
                     /translation="MADQHEKTGARLAEVARLFLRMGVTAFGGPAAHLAMMHDETVIR
                     RKWLTDSQFLELVGATNLIPGPNSTEMAIHLGYLRAGWRGLLLAGFCFVVPAMLIVLL
                     LAGLYARYGSVPQAQWLLDGVKPVIIAIILQALWMLGRKALKTPLAAAAAALVLAGCF
                     AGGNEIALLFGAGAGVMLLRNIRRIQPAMFAAALPLAPVVSAAPAGVPFGLMRLFLTF
                     LKIGSVMYGSGYVLLAFLRADFVVRYGWLTDQQMLDAIAIGQVTPGPLFTSATFIGYQ
                     MGGPPAALAATLGIFLPSFIFVGLFNPLIPRMRKSPWAASLLDGVNAASLGLMAAVTW
                     QLGMSSLTDPASVMIALLAGLLLIRFRTSSTWLIAGGALIGFLRWISQV"
     gene            14690..15550
                     /locus_tag="PKK36_05090"
     CDS             14690..15550
                     /locus_tag="PKK36_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93969.1"
                     /translation="MKAYKTAILGVLVTAMVFPLRAADDKTGEITLDQISARAEENRK
                     QYEQLIKSLERTVKESQQRVTVLKQQKDNWKTEISSLETEIGDLQAERKRREKEIAQQ
                     EELVIGLQQKFKLLAETIDIYENAADFQAQIQEKDVMINALQQRARALEKESAAARKS
                     ADKLLAETRRESESWQKMTRDYQQKLAAQEKRTVQLEQELSESSARIREQGKKLDEET
                     TARRFLEQVVADRDRQLSEMHALLKETEEEKNAAKADALATLELLESFLADRDNLKGA
                     VERVRGNLGR"
     gene            15611..16408
                     /locus_tag="PKK36_05095"
     CDS             15611..16408
                     /locus_tag="PKK36_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR93970.1"
                     /translation="MKLQISRPIAFFDIESTGVNTMQDRVIDLAVVKLMPDGSREVFS
                     WRLNPGMPIPKGSTAIHGITDADVANSPSFADKAGEILACFENCDLGGFNVLGYDIPL
                     LQAEFARVNMTFDMTGRNVVDGARIFHQKERRTLTAALKFYCSDEHTDAHGALADVEA
                     TIRVVEGQLDRYDDLSGNVEELSAFCNPKNPNAIDAQGKIVWNDDNEAAVNFGKHQGQ
                     TLRWLVRNEAGFIQWVLKKDFSEDIKTILRAALAGNFPVRKMEEPEA"
     gene            16457..17119
                     /locus_tag="PKK36_05100"
     CDS             16457..17119
                     /locus_tag="PKK36_05100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016856071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR93971.1"
                     /translation="MDCPLCNAPADFFVRTSLRDWHQCSRCALRFVPAAQHPPAGIEK
                     ARYDLHRNDPADSGYVAYLSRLFLPLRERLMPGARGLDYGSGPAPVLARRFTDAGFPC
                     EPYDVFYAPETGRLASQYDFLACSEAIEHFFHPREEFERFLKLVRPGGWIGIMTQLYD
                     EAAQPFDQWHYTRDVTHVCIFARATFQWLEKRYGLSAEYHPRGVVLFRNTQTASRGND
                     NT"
     gene            complement(17361..>18117)
                     /locus_tag="PKK36_05105"
     CDS             complement(17361..>18117)
                     /locus_tag="PKK36_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNR93972.1"
                     /translation="LMPCSAKKFECNRPEMSDSGFKDIYYGLTTRELAQMISEAGINL
                     PAMEKSDFDDPFGTATGSGVIFGATGGVMEAALRTVIELVTGKKVENFFDHADIIPVR
                     GMEGIRYAEIPIKEVGPVPDLVKHVVKDWKWLKGATLKVAVAHGTANAKRVMENIKAG
                     GKLSECHFIEFMACPGGCIGGGGQPIPTSPAIRAARARAIYAEDTAYEVRKSHENPAI
                     ERIYNEFLKEGPCSHLAHKLLHTHYTPRGKYIV"
BASE COUNT         3956 a         4897 c         5498 g         3766 t
ORIGIN      
        1 agacggacac caaccggctg gatattgtcg acaccaactt cctcatgcgc attggctcgg
       61 tcagtttcta tgtcaatgat gcgacgaacg atgagcagga tgtctggtgc ctggctcccg
      121 gtgatgatgt caataccggg cttgacccga ctgtgcccat gttgacgatt caggcgctgc
      181 tcgacacata tgatattgca cccggcgatt ttatctttgt tgatgcgggt tattactccc
      241 tggaggacga tctgatcatc aacgctctgg attcgggggt ggccacgtcc cgagtgcaga
      301 tcaccggcgc acctcaccgg ctcacggtat ttgaccgtgg cgatacgacc aatgaaacca
      361 catacggcat tgtgttcaac agcgcaacct gtgtcaccat atccaatatc gttgttcaga
      421 atgcagatgt cggtgtggat ttctatcgca gcctctcctg cgccctcagc gattcgctga
      481 ttcgcgactg cggacagtcc ggcgttcgat tcttccagac aaagtccaac ctgctccagc
      541 gcacggtgat cactcgcagc ggggactccg gcgtacagga tcagctcagt acatacaact
      601 atcttgacgg gtgcgtggtc tgggccagcg gggcgaatgc tattcagggt gttaacagtc
      661 tgctctgtgt gagcaattcg gttctgcatg cttcggggtc cgaccggctt tgctatcaga
      721 attcgaccaa tgcagtgttc ctgtcggatt acaacaacct ctttgttgag aacggtgcgt
      781 tctacgggaa gagtccgtct cccgttggcc tgattggaga gggcatgcct caatggattc
      841 tctataccac tcaggatgtg cattcgctga gttttgaccc catgtttgcc gatccggaca
      901 atgatgattt tcatccgatg agtccggagg ggcgttatgt aacgggaaca tttgtgcagg
      961 tggatgccgc attctccccg ctcattgacg cgggcaatcc gggcaccatc gtcaccaatg
     1021 aaacagaccc caacggcggc cgcataaaca tcggcgcgtt tgggaatacg gcacaggcca
     1081 gccgaagccg gacgaatgca tgggttctgg ccgtttacgc ctcggcgggc ggtcgcgttg
     1141 ccggcggggc tcctgtactg ctgacatggc tgcaggggaa tctggatgcc accaaccggg
     1201 tgacgctgga atattcgtgg gataacggaa caacgtggac caatattgtg tcggggattc
     1261 cggccggcga cgggcagtat ctgtgggacg tggatgaaga agaatcgggc tcgccgaaat
     1321 atcccgccag tcccacggcc cgctggcgcg tcttcgttga agccgatccc tcagttgttg
     1381 acgataccga tgtgttcttc tcgctgaagg gtccgttcac cttctacatc aatgacctga
     1441 acatatccaa tgacgtttat actggcgcag caggaaacga tgcgaatctg gggattctgt
     1501 cgaatgcgcc caaactgacg ctgacttccc tgatacagca gtgggatatt gagggcggtg
     1561 ataccattgt tctggacacg ggcctgtata tcatctccaa tgaggttgtc attacgtccg
     1621 ccgatgcggg gaaggccggg tcccccgtgc tgctgatggg aagcacgaac gccggcggcg
     1681 cgcttctgtc ccgttcgacc aataacacca gcctgctgag gatcaacgcg gatcacatgg
     1741 atgtggcggg cctctctgtg cggggcgggt tcctgagggc taacggcaac aacctgaatc
     1801 tgtccgactt gaatttgagc accggcacgg tcgagatcac atctcggtct tcgacggtaa
     1861 cccgcgtgac tcaagtcggc tcatacttcc gcctgcaggg tagcgcggac atggtgctgc
     1921 agcggctgaa catgcggaat gcccgcttgg aaacaaaggg cagctcggat attctggttg
     1981 aaaacgcgtt gatctatggg acctccacca acgctgtgct cgtggatgag tctcagcggt
     2041 ttacgatgcg caattgcaca ttgtctgtga acggcaacca gttcctgcag tccggctcct
     2101 cgctgtcgga gatagagaac aacatcatgg ttgcaaatgg agcaagccgg ttctgcattc
     2161 tgcagctggg cggggctctt gattcggatt acaatacgct ggtgacccgg aacggagcat
     2221 ggttcggcaa tgccgaggga cagtgggaga agctgatcta ctggcaggat gagtcgggac
     2281 tggatgtcca cagcctgccg catgagccgc tctttgttaa tgaaggaggc ggagattatc
     2341 atttgcagtc ctatgtcgga agctggaacg ggacaggctg gtccaccaat gcaaatcatt
     2401 cgcccgcaat tgacgccggc aacccgtcca cggtatacac caatgaaccg tacccgcgcg
     2461 gcccagcggt aaacatgggc gcctatggcg gaaccgagga ggcttcaaag agcctgctgg
     2521 atccctggct gctggccctg accgtgagcg acggcggtgt gttgagcggg accaacaccc
     2581 tctactggct gtcgggcaat atcatcccaa caaatacgct gcggcttgat tattcgctgg
     2641 acgggggcgt cacgtggatc acctttgcgc ctggactgga ttccaccttg aacgagtatg
     2701 cctgggacac cacggcaaac atcacaaaca gcattgaaac gctctggcgt gtggtggtgg
     2761 aggcgaacac caatgtgttt gatgaaaccg atgagccgtt ctccgtgcgc aatgccccga
     2821 tatatttcta tgtcaatgac accaataccg caggggatgt attctgcggc gcacccggtt
     2881 ccgctctgaa tgacggtctt acaacgaaca cgcccaagct gaccattcag gggattctgg
     2941 atgcctatga cctggagggt tatgacacgg tatttgttga cgcgggctac tacaaactgg
     3001 gtacagagac ccgaatgata tggtctgatg cgggcaaggc ggatatcggg aatctcctga
     3061 tccagggggc tggaacggga accgtcattg cgggctggag cagggccccg gcggccacgg
     3121 ccttgcgcat ttacggaaat tacattgatt tgcgggactt cacgatcagc gatgccgggg
     3181 taggaattct tatggattcc aaccattatg tgacggccga gaaccttttg atgatcagca
     3241 atgcgctggg catcaatctg cagtccggcg caaattacga cattcgcaat tcgctcttcc
     3301 tgtataacac caacagcgcc gtccagatgg atcgcgtgac cgatgcctct gtagaaaaca
     3361 acaccttcta tggcaatgct cccgccgcga tttcccttat gtcgtctgtg aacagtgtgc
     3421 tgcggaacaa catattcggc cttaccgccg cggaggcata tgcctatgga ggaacatcca
     3481 acgatgtggc gaacaatgtg acaatggatt acaatgtcta ttatttttcc gagccgggct
     3541 acatttacag cacgttcaac acgctgcttc cgtggcagct gaactacggg catgattacc
     3601 gcagcgcgat caccaacccg ttgtttgccg cggtggataa ccgggatttc catctgcagt
     3661 cagcggctgg ccgctatctg ccgggtaccg gattcgtgac gacggacagt gagacgtcct
     3721 gggcgattga caagggcgat ccgtgggctg attacagcct tgagcccgac gaaaacgggg
     3781 ctcgaatcaa catcggccgc tatggcggaa cagaagaagc aagcaaggga agcacgaatg
     3841 tggtggtcta cgcgcgctcg ctgaatgatc cggtgtttat caccgatacc aacagtatct
     3901 ggccgttgat ttggaccgcc ctgaatgtgc ccaccggcac gctggtcacg gtgcagtact
     3961 cgggtgatgg tggggtgacc tgggaagaca ttgccacagg attgaatgtt tatgaggaat
     4021 atatcatctg gcagccgacc tttgaggata actcttacag cgctttgtgg cgggtggttg
     4081 actcaaccaa tgctccggta tatgtggaca ccaacgacac cgaggcgggc ctgttctttg
     4141 gaacctttga gatatccgag gcaaacaaca tggaaggcac caacatgcat tacatcatct
     4201 ggcgcggggc atggaatgag gcctatcagg ttgaatattc atacggattc acccagtttg
     4261 gagatatcat ctggtcaaat tgtgtggacg gagtgggatc gaatcaggcg gcctaccggt
     4321 tcaccacatc aggcgggaat ttccgctacg aggacattga aagtccaaac cagtccaacc
     4381 ggctgtaccg ggtcaagtgg attcagagcc cgtagccggg agatatgtta atcgcggagg
     4441 gcgagggcaa cctcgccctt tgctttgaag ggaggttcga ttgcgaatta tcagcggtca
     4501 gctcggcggg aggaccatca ggatgccccg gggggatgtc cggcccacgc aggacaaggt
     4561 ccgggaagca atcttttcct cccttgctcc gctgattccc ggggcgcgcg ttctggacct
     4621 gtttgcgggc agcggagcca tgggactgga ggcatggagt cggggggcgg cggcggtgtg
     4681 ctgggtggag cagaatcggt ctgtttttcg taccctgcag cagaatgtca gggagttgtg
     4741 cggggacagt ccgcaggcgc actgtgtgtg cgccgatgcc ttcagcttcc ttcggaatgg
     4801 gggcggagtg aaatatgata ttgttttttg cgatcctccg tatgggaaga cggagagggg
     4861 ccgtcttgaa caaatgctga acgttctggg gggcagtccg tccttgcctc cgggggccct
     4921 gttggtttat gaacatgggg cagcggaggc tcctgcggat tctcccttgt gggagctgaa
     4981 acggagccgc cgctacggga aaacaggtgt cgttatttac cagaagttga gcgaacggag
     5041 agattgcggt gagcactaaa gcgatttatg cgggaacatt cgaccctttg acgctggggc
     5101 atatggattt gatccgtcga gcctcgggta tttttgatga actgattctc gcggtggccg
     5161 ggagcacccg gaagcagacg cttttttcac ttgaggagcg cgtggccatg gcgacggaag
     5221 tcgtgacccc gtttaaaaat gtcacggtgg agggctttaa cggcctgctg gtggattacg
     5281 cccggtcgag aaagatccat gtcgtggtga gggggctgcg ggctttttcc gatttcgaat
     5341 ttgagtttca aatggcgttg atgaaccgga gcatggcttc tgacgtggaa accatgtttc
     5401 tgatgcccaa gcaggactac agttatgtca gttccagttc cgtgcgcgaa gtggcggaat
     5461 tgggcggaga tgtaaccgct tttgttccgc ccgctgtcaa acgggcgctt gatttaaagc
     5521 tgaaaaaccg ggtttaacac tgcatgcgca tagaaacagc cagactgact ccggccggaa
     5581 gcctgttcaa gggtgagcat ccagcctctg ttcttgaact ggagcacgaa gcgctgctcc
     5641 acgcggaaga gccggttgtt tacgagctga cggttcgtct ggtttcccac gaagtgctgg
     5701 tgcagggccg gatttccacc aaaatagagg tccaatgctc aagatgcggc gaatttttct
     5761 cgacattggt ggaagattcc tctttcctgt gcacttacga aattgagccg gggcaggact
     5821 cactggatat caccgaagat atccgcgagg cgatcctgtt gcttataccg gccttccccg
     5881 tatgcagaga agactgcagg ggagtatgta tgcagtgcgg ccggaatttg aatgagggcc
     5941 cctgcagttg taagaagaaa gtattgaatg atccgtggga gaaactgaac cggctggatc
     6001 tgtaaaaaac aggctggatg gtggaattca gcaacgatca ggaggcattt tatggccgta
     6061 ccgaaaagaa agaaatcaaa gagcaagaag agaatgcgga aacgctcgct ttccggcgag
     6121 attctggctg cccgtccctg cccgcagtgc ggcgcccctg tgcagtctca tcgagcctgt
     6181 ccgtcgtgcg gcacctacaa gggccggcag gttgtttcgg tgaccgccga cgagtagagc
     6241 ccatgcgcat tgctgttgat gccatgggcg gggattttgc ccctgcccag aatgtggagg
     6301 gtgtgctgga ggcgctggaa actctgcccc agatcgagca tctcttcctg gttgggattg
     6361 aagaagcgat catcaaggcg cttccgcccg ggcgttcttt cccttcaaaa gtggagattg
     6421 tcccggcgtc tcaggtggtg gaaatgcacg aatctcccgc ggtggctgtt cggcgcaaga
     6481 aggattcctc catcggccgc gccattgatc tggtgaagca ggggaaggcg gatgccgtat
     6541 tttccgccgg caacacagga gcggcggtgg ccgcctgcac cctgaagctc aggacgcttg
     6601 agggcgttga ccgtccggcc attgcgacgg ttatgcccac caccggcgac aagccgtttg
     6661 ttctgattga tgccggggcc aatacggact gcgaaccgga actcctctat cagtttgctg
     6721 tcatgggaag catctatgcg cgcgagatca taggaatttc caatccgcga gtcggccttc
     6781 tcagcatcgg cgacgaggat gccaagggca atgaggaaac caaggcggct tttgccatgc
     6841 ttgaaaaatc cagcctgaat ttcatcggga acgtggaggg ccatgatctt tacagcggag
     6901 aggttgacgt ggtggcctgt gacgggtttg tcggcaatgt ggtcctgaaa accagcgaaa
     6961 gtgcggccca ttccatcagc cggtggctta agattgagct cacaaggaat tacctccgga
     7021 tgctgggcgc gtttttgagc cagggggcct tcaagacact gaaaaagaaa ctggatccgg
     7081 cggcctacgg cggcgcgccc ctgctgggca ccaacggagt ctgcatcatc gggcacggat
     7141 cgtcttcgcc ccatgcggta cgcaacggta ttcgcgtggc ctgtgaatcg gtcaaccaca
     7201 agattaatca tcacatcgtc agcgcgctgg ctgactcggc gtcgggagaa aagagtgagt
     7261 aaaaaggaaa aattctctga tgcaggacgc ctgcgcaccg tctccattgc cggcaccggc
     7321 tcctatatgc cggagcggat tatgacgaat gccgagctcg aaaccatggt ggagacttcc
     7381 gccgaatgga tcgtgacgcg aacaggcatt gcggaacgcc gcctcgccgg cgaaagtgaa
     7441 accacttccg atatgggggc cgaagccgcc cgccgcgcgc tggcggatgc cggggtcagc
     7501 ccggaagaag tggatctgat tgtgctggcc accatcaccc ccgacctgcc gtttcccaac
     7561 actgcttgtc tcgttcagga taaaatcggc gcgaaaaatg ccgcctgttt cagccttgag
     7621 gcggcctgtt ccggatttgt tttcgcaatg gaaaccgcca ggcagtttgt ggcgaccggc
     7681 gcggcaaaga cggcgctggt ggtcgcggcc gagaagatga gcagcatcat tgactggacc
     7741 gatcgagaga cctgtgtgct gtttggtgac ggggcgggag cctgtgtcct gaaggcggcc
     7801 gagggggata agggtctggt acacgccgtg ctgggctcaa acggcgcgct ggctgaactg
     7861 ctcatggttc cgggcggcgg ttcgcgcctt cccttttccg aagaagtcct gcgtgaacgc
     7921 aaccattacc tgaaaatgga aggccgtgag gttttcaagc atgcggtcaa aagcatgacg
     7981 gccattgcac agcaggtttg cgccgagaag gggctgacca caaaggatgt ggcctgcatc
     8041 gtgccgcatc aggccaacat ccgcatcatt gaggccatcg gccagcggct gggggagccg
     8101 aaggagaagt ttttcatcaa tctcgacaga tatggaaaca catcgggcgc ctccgtgatc
     8161 gtcgcgctgg acgaagctgt ccggtccggc gtcattaaaa gaggggatct ggtcatgctg
     8221 ctggtgtttg gcggcggatt cacctggggg gcttcacttc tggagtggtc aaaatgatga
     8281 agacagcatt gatatttccc gggcagggag cccaggcagt gggcatggga agggacctcg
     8341 ccgaagcttt tcccgcctgc aaagaactct ttgatcgcgc cggcgccgtg ctgggctgca
     8401 atctggcgca gctttgcttt gaaggccctg aggagctgct cacgcggtct gacaatgccc
     8461 agccggcaat ttttacggcc agcattgcct gtctggaagc gcttaagctg gaatatcccg
     8521 gcttttcttt cgacgtgacc gcgggtttga gctcgggcga atgggctgct ctgcatgccg
     8581 ccggagtgct cagctttgag gatgccatcc gaattctcgt cgcccgcggc cgtttcatga
     8641 tcgaggcctg tcaccagcag cccggggcca tgctgagtgt catgaacctg gatctggaaa
     8701 ttcttcggaa aatcagtgca gaagccggca ttgaaatcgc caatctgaat tccccgggcc
     8761 agaccgtgct ttcgggccgg gttgaaggca ttgagcgggc ggaaatactg gccaaagagg
     8821 cgggggccaa gcgcgcaatc aggctcaatg tctccggggc ttttcattcg tcgctgatga
     8881 attcggcagc tgatcgtctg gccgaagtcc tggcggggat ttcattcaga accccttctg
     8941 ttcccgtggt ttccaatgtg actgcggagc ctcacgggac cctggagacg attccggccc
     9001 tgatggtgcg ccaggtcaat tcgtccgtcc gctgggtgga gtcggtcgaa tggatgaagg
     9061 ctcacggtgt ttcccgttac attgaatgcg ggcccggaaa agtcctgtcc ggtctggtaa
     9121 agcgcattga caaagaagcc atctgcctta acatactgga tcttgagtct ttaagaagct
     9181 cgcaggccgc attacaggga attggatgat ggatttgaac ggaaaagtgg caatagtaac
     9241 gggtgccgcc cggggaatcg gacaggccgt ggccgtcaga ctggcgtcgg caggcgcgga
     9301 tatcgcgctg tgcgatgttc aggctggatg gctggatgaa acggccggcc ggatcgcgga
     9361 acttggccgc cgcgctgaaa agtatgccgt tgacgtgtcc agcagcgcgg acgttgaggc
     9421 cgccatttcc caaattcatc aggatttcgg ccacattgat gttctggtaa acaacgccgg
     9481 catcacaaag gacggtttgc tggcgcgcat gagcgagcag gactgggatg ccgttttgaa
     9541 tgtgaacctg aagggggcat tccttttctg caaggcggct tcgcgcatca tgatgaagca
     9601 gcggaccgga agcattgtga acatggcttc cattatcggg ctgatcggca atgcgggcca
     9661 gtgcaattat gccgcgtcca aaggcggcct gatctccctg acgaaatcgg tggccaagga
     9721 gctggcgggc cggaatgttc gcgccaatgc cgttgccccc ggcttcatag agaccaaaat
     9781 gaccgaaaag ctgtccccgg atgtccagca gaagatgctg gaggccatcc cgatgaagcg
     9841 attcgggaag cccgaagatg tggccaatgt ggttctgttc ctcgccagcg atgcgtccgc
     9901 ctacatgacc ggacagacgt tgaccgtttc gggcggcatg gtgatgtgat tttgcacggt
     9961 aaaaggaatt ccggatcagt gagtttgttt tgcctgtata gttgaaaata gtgcgatggt
    10021 ttaaccaaaa acaggaggct agaaatggca cttgaagata aagtgaaaga aatcattgtt
    10081 gagcagctgg gtgttaacgc cgatcaggtg acccccgagg cttcttttat tgaagacctg
    10141 ggcgccgact cgctggacac ggtcgagctt gtgatggctt ttgaagaaga attcggcgcg
    10201 gaaattccgg atgaagatgc cgagaagctc acgagcgtcg gcgcggtcat caattacctt
    10261 aaagaaaaag ggcttggcgc ctaacagccg caggccgctg attttgaacc gggccggact
    10321 gaacactccc gcccggttca ttttcacgtt tattccgttt aggggaaggc agggaaggaa
    10381 ccatgacaag aaaagtggct gtcaccggtt tgggtattgt gtctccgctc ggatgcagtg
    10441 tagacacggt ctggaatcgc ctgatgaacg gcgagtccgg catcggcccc atcacctatt
    10501 ttgacacaac cctcttcgac gccaaaatcg gcggacaggt gaaggagttt gatgtggatg
    10561 ccttcatccc caagaaggag cagcgccgca tggatccgtt ctgccactac gggatcgccg
    10621 cggccaaaat ggcggtttcc gatgccggcc ttgaaatgga aaaagaggat ccatggcgcg
    10681 tgggcgtcat ggtcagctcc ggcatcgggg ggcttcaggt tgtcgaggtt cagtgcaaca
    10741 acttcctgca gagagggccg tcgcggttct cccccttcat gatccctcag atgatcacga
    10801 atatcctctc cgggcgaatt gccattgaac acggcatgaa aggccccaat ttctgcgtcg
    10861 tgtcggcctg cgcatccgcc acccattcca tcggcgaaag cctgcgcatc attcagagcg
    10921 gcgacgccga tgtgatgctc accggcggcg cggaagcccc ggtctgcgtc ctctcggtgg
    10981 gcggattctg tgccatgcgg gcgctcagca cccgcaatga cgatccgacc cgggccagcc
    11041 gcccgtttga ccgggaccgt gacgggtttg tttgtgcaga gggggcgggg gtgcttgtcc
    11101 tggaggaaat ggagcacgcc cgggcacgcg gggcgaaaat ctactgtgag ctggccggtt
    11161 acggccgcac ctgcgacgct tatcacatca ccgcgcctga cgagggcgct gaagcggccg
    11221 caaagggcat cagccttgct gtggcggatg ccgggatgaa tcccgatcag gtggaataca
    11281 tcaatgccca cggaacgagc actccgctga atgacaaggg cgagacgctg gccatcaaaa
    11341 aggctctcgg cgaaaccgcg gcccgcaaaa ccatggtggg gtcaaccaag tccatgaccg
    11401 gccatctgct gggtgccgcc ggcgcggtgg aagcgatttt ctgctccatg gctctgaaaa
    11461 agggacaaat tcccgcaacc atcaatctgg aaaatccgga tcccgcatgt gatttggact
    11521 atgtcccgaa caccgcgcgc gaagtgccca ttaaaacggc cttgagcaat tcccttggat
    11581 ttgggggaca caacgccacc gttgccttca aggcggtctg acggctcatg tgtttccggg
    11641 cccgccttgc tgcggggcca tgcgtgtggt gcccatttca tgtgaaatga gcccgggcgg
    11701 aaagaaccgc ttgttttgcg tccttttttt ctgcaatctt cgttccgggt gaatggcttt
    11761 taacaatgaa ggagaaacca tcatggataa tacaacagct cagtccgtta tagatgcaca
    11821 ggtcgaaatt acgggaacca ttaaaagcgg cgggtccatc aagattgacg gcaaactgaa
    11881 gggcgacctc cagtgcgaag gggatgcaac gatcgggaaa agcgcggtga ttaccggaaa
    11941 cctgacggtg aactccgtgg cggtggccgg cgccgtgaca ggtaacatcg tggccaagga
    12001 ccgcatccag atgcagtcca gcgcgcgagt gatgggcgat atcaaagcca agcgtcttca
    12061 cgtggaggac ggagtcacat ttgtcgggaa gtcagaggtg aatccttccg gcgggcctgt
    12121 ttcttcaacg cccaagccgg aggcggagga atccaggtcc gggatgttca gcgggaaaaa
    12181 gtaaactcgc gaactgattt cacaccggag gaatggagtg gctccgcgca aagccggaga
    12241 ggttgacgga ttcatggctt tgaaggcggc ccagcgggcc gtcaaaaaag ccccggccgc
    12301 gcctgtgccg ccagcgcccc cctcagtcgc aaaagattcc ggcggcgcgc agaagaagat
    12361 cggttcacac attggaaaga ccacgatgcc gacccggcac gaagtggtct gctatgaatg
    12421 cggtttcttc tttcagatga ccggccgccc gaagagtacc ttctgtccga agtgcaaggc
    12481 gaacctgacc ttctcggatc actcgatcga aacggtatgg tccgaatccc ttaagacggc
    12541 cggctccatt ctgattggac ccgccggagt cgtgacagcg ggggatttca ttgcgacgga
    12601 tatcattgtc gaaggccgcg tggaggacgg ggcgctgatg gcttcccgtt ttctggaggt
    12661 gagggccggg gcgacggtca gcgagcaggc catgaaaagc cgtgatctcc ggattgccgc
    12721 cggcgcaagt gtgaaactga cctcggcggc ggtctaccgc aatgtggaca ttgccggccg
    12781 gctccgtgcg cggcttaaag cctccgggac cgtgaatatt tccgcatcag gctgctttga
    12841 aggggaactc accggcgcgc atctcacggt tgaagagggc gggggcctgc gcgcgcgcct
    12901 gcatgttgaa gcggatgtaa agaaataacg gttgatcagg gcgcaggggg gccgcccggg
    12961 ttgaccagag acttcatttc ccggacccat tgcagatact cccgggccat cggaaacgct
    13021 ggttcaagca taacggcgat ttccagatcc ttttcggcat caataaaatt gccttctcca
    13081 gtggcggcca cgccgcgcca gaaataacca tcgggcttgt agcggttcat gcggagcagt
    13141 tcctccgcat tcgcccgcag cgggaattcg ttggttgtgc tgaacacctc gtggttcttc
    13201 tgtgaaagct ccgcaaacgc cttttcctcc ggggaaagct cctgttcggg cgccctccgc
    13261 gcgcagcctg cgataagggc gatcgcgctg acggcggcaa gaagatatgc tgcttttttc
    13321 atgaaagtac tatagcatca catggtcttc tgctcaagcg aatcacatgg agcggttcgg
    13381 acgaaagttc acacctgact gatccatctc aggaatccga tcaatgctcc tcctgcaatc
    13441 agccaggtgg aacttgtgcg gaaacgaatc agcagcagtc ctgcgagcag cgcgatcata
    13501 acgctggccg ggtctgtcag cgaagacatg cccagctgcc atgtcaccgc cgccatcagg
    13561 cccagagagg ccgcattcac cccgtccagc aggctcgccg cccacggcga tttccgcatc
    13621 cgggggatga ggggattgaa gagcccaaca aaaatgaacg agggcagaaa tattccgaga
    13681 gtggccgcca gcgcggcggg cgggccgccc atctgatatc cgatgaaggt tgcggatgtg
    13741 aacagcggcc cgggcgtaac ctgtccaatg gctatcgcgt ccagcatctg ctgatcggtc
    13801 agccatccgt aacgcacaac gaaatcggcc cgcaggaatg caagcagcac atatccgctg
    13861 ccgtacatga ccgatccgat tttcaggaac gtgagaaaga gccgcatcag tccgaaaggg
    13921 actccggcgg gcgcggcaga aaccacgggg gcgaggggga gggcagcggc gaacatggcc
    13981 ggctggatcc tccgaatatt gcgcagcagc attacgcctg ccccggcgcc gaaaagcagc
    14041 gcgatttcat tccctccggc aaagcaaccc gcaagaacaa gcgcggccgc ggctgcggcc
    14101 agaggggttt tgagcgcttt gcggcccagc atccagaggg cctgcagaat gatggctatg
    14161 atgaccggtt tcactccatc aagaagccac tgggcctgcg gcacacttcc ataacgggca
    14221 tacaggccgg ccagcagaag cacgatcagc atggcgggaa ccacaaaaca gaaaccggcc
    14281 agaagcagcc cgcgccagcc cgcgcgcaaa tagccgagat ggatggccat ttccgttgaa
    14341 ttcggtccgg gtatcaggtt ggtcgcgccg accagttcca gaaactgact gtcggtcagc
    14401 catttccggc gaatcacagt ctcatcgtgc atcatggcca gatgcgccgc cggtcccccg
    14461 aaggcggtaa cccccatgcg cagaaaaagt cgggccactt ccgccagccg tgcgcctgtt
    14521 ttctcgtgtt gatcagccat gccgccggtt tatagcatcc ggtctttccg gggctcaatc
    14581 ttttctctaa cccgataatc ccctgatcag gtctttacct cttgacctgc gacggcaatg
    14641 ccgctacaag ggcacggaat tttagaccaa aacagaagga atggttcgaa tgaaggcata
    14701 taaaactgca attctgggtg tcctggtgac ggcaatggtt tttcccctgc gggcagcgga
    14761 tgataaaacg ggggagatca cgcttgatca gatttccgcc cgcgcggagg aaaaccggaa
    14821 gcagtatgag cagctcatta aatcgcttga acgaacggta aaagaaagcc agcagcgggt
    14881 cactgtgctg aagcagcaga aggacaactg gaagacagag atttcctcgc tggagacgga
    14941 gattggcgac ctgcaggcgg agcggaaacg gcgcgaaaag gagatcgccc agcaggagga
    15001 gcttgtcatt ggactgcagc agaaattcaa actgctggct gaaaccatcg acatttatga
    15061 aaacgccgca gatttccagg cccagattca ggagaaggat gtcatgatca atgcccttca
    15121 gcagcgcgcg cgcgcgctgg agaaggaatc cgccgccgcc cggaaaagcg ccgacaagct
    15181 tctggcagag acccgccgcg aatcggaatc ctggcagaaa atgacccgcg actatcagca
    15241 gaaactggcg gcccaggaaa aacgaacggt ccagcttgag caggagcttt ctgaaagctc
    15301 cgcgcgcatc agggaacagg gcaagaaact ggacgaagaa accaccgcac gccgttttct
    15361 cgagcaggtt gtggccgacc gcgaccgcca gttgtccgaa atgcatgccc tgctgaaaga
    15421 aactgaagag gagaaaaacg cggcgaaagc cgatgccctt gccacgctgg aactgctgga
    15481 gtcgtttctg gccgaccgcg acaatctgaa gggcgccgtt gagcgtgtgc gcggaaatct
    15541 cggccgctga cggaagttat ttgagttcgt gattgtgcgg gatgcaccgc ccggatagaa
    15601 tgactgcact atgaaacttc aaatcagcag accgatcgca tttttcgaca ttgaatccac
    15661 gggcgtgaat accatgcagg acagggtcat tgacctggct gtggttaaac tgatgcccga
    15721 cggatcccgg gaggtcttct cctggcggct caatccgggc atgccgatcc cgaaaggctc
    15781 cacggccata cacggcatca ctgacgccga tgtcgcgaac agtccctcat ttgccgacaa
    15841 ggccggcgag attctggcct gtttcgagaa ttgcgacctt ggagggttca atgtgctggg
    15901 ttatgacatt ccgctgctcc aggcggagtt cgcccgcgtc aacatgacct ttgacatgac
    15961 cgggcgcaat gttgtggacg gcgcgcgcat tttccaccag aaggagcgcc gcacgctgac
    16021 ggccgccctc aagttctact gcagcgatga acacaccgat gcgcatggtg cactggccga
    16081 cgtggaggcc accattcgcg tggtggaggg acagctcgat cgttacgacg atctctccgg
    16141 aaatgtggaa gagctttcgg ctttctgcaa tccgaagaat cccaatgcca ttgacgcgca
    16201 ggggaaaatt gtctggaacg acgacaacga ggcggccgtc aacttcggca agcatcaggg
    16261 acagacactg cgctggctgg tgcgcaacga ggccggtttc attcagtggg ttctcaagaa
    16321 ggatttttct gaagacatca aaaccattct gcgtgccgcc ctggcgggca atttccccgt
    16381 ccgtaaaatg gaagaacccg aagcctgaat tttcggccgg cgtgtaggtt gagccgtgaa
    16441 cgggagtgga tccaatatgg actgtccgct gtgcaatgcg ccggcggact tttttgttcg
    16501 gacttccctg cgtgactggc atcaatgctc ccggtgcgcg ctccggtttg ttccggcggc
    16561 acagcatccc ccggcaggga ttgaaaaagc gcgctatgac ctgcaccgca atgacccggc
    16621 cgacagcgga tatgtggcct acctgagccg gctgttcctg ccgctccggg aaaggctcat
    16681 gcccggcgcc cgcggcctgg actacggcag cggtccggcg ccggtgctgg cacggcgttt
    16741 caccgatgcc ggatttccct gcgaacccta cgatgtcttt tacgcccccg aaactgggcg
    16801 gctcgcctct cagtacgatt ttctggcctg ctctgaagcc attgaacatt tcttccatcc
    16861 ccgggaggaa tttgagcgtt ttctgaaact ggttcgcccg ggcggctgga ttggaatcat
    16921 gacccagctg tatgatgagg cggcccagcc gtttgatcag tggcactaca cccgcgacgt
    16981 gacccatgtc tgcatctttg cccgcgccac cttccagtgg ctggaaaaga gatacggcct
    17041 cagcgccgaa tatcatcccc gcggggttgt cctgttccgg aacacccaga ccgcatcacg
    17101 cggaaacgac aacacttaat ataggactaa aacagtccta tattgatgat ggcccgaaag
    17161 atgcagtcca ccgcgatcag atatgcagtc cgaaggcaaa aaacgcgaaa aaacgcgaaa
    17221 aaacgccgga aaacgcctaa aaatcgccaa aaagtgtcaa aaaacgctta aaattggcca
    17281 aaaacgcaga ttttcaagaa agaacgcaca aaaaaaggcc gcgcgatgcg cggccttttt
    17341 ttgtccattc aaagggcgga tcagacaata tattttccgc ggggggtgta gtgagtatgc
    17401 agcagcttgt gagcaaggtg gctgcacggc ccctccttca ggaattcgtt gtaaatccgc
    17461 tcgatggccg gattttcgtg cgacttgcgc acttcatagg cggtatcctc ggcgtagatg
    17521 gcgcgcgcgc gggccgcgcg gattgccggg ctcgtcggga tcggctgccc gcctcctccg
    17581 atgcatccgc cgggacaggc catgaattcg atgaaatggc attcgctgag tttgccgccc
    17641 gctttgatgt tttccatcac gcgcttggcg ttggcggttc catgggccac cgcaaccttc
    17701 agtgttgcac ctttcagcca cttccagtct ttcacgacgt gtttcacgag atcgggcaca
    17761 ggaccgactt ccttgatggg gatttccgcg tagcggatgc cctccatgcc gcgcacgggg
    17821 atgatgtccg catgatcaaa gaagttctcc accttcttcc ctgtgaccag ttcaatgacc
    17881 gttcgaaggg cggcctccat cacgccgccg gtcgcgccga agatcacgcc ggaaccggtg
    17941 gcggtgccaa agggatcatc gaagtcggac ttctccatgg cgggcaggtt gatgccggcc
    18001 tcgctgatca tctgcgccag ctcgcgtgtg gtcaggccgt agtaaatgtc cttgaagccg
    18061 ctgtcgctca tttccgggcg gttgcactcg aacttcttgg cggagcaggg catcagg
//
LOCUS       DAOQWR010000043        17754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3570_length_17754_cov_5.415730, whole genome shotgun sequence.
ACCESSION   DAOQWR010000043 DAOQWR010000000
VERSION     DAOQWR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17754
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_17754_cov_5.415730"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1400
                     /locus_tag="PKK36_05110"
     CDS             369..1400
                     /locus_tag="PKK36_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR93973.1"
                     /translation="MLSQLFGLFSNDIGIDLGTANTLVYVRDRGIVLREPSVVAIQSG
                     TNRVLAVGEEAKRMLGRTPGSIVAIRPMKAGVIADFEITEAMLRYFIRKVHNRGKLVR
                     PRVIVAVPSGITEVEKRAVKDSAAHAGAREVYLIEEPMAAAIGVGLPVQEPAGNMIVD
                     IGGGTTEVALISLAGIVFSRSVRVGGDQMDESIVQHMKRVYNLMIGERTAEEIKISIG
                     SAYPMKEETTMEVKGRDLVAGLPKTLTVTSEEIREALHEPVATIVESIRITLERCPPE
                     LSADLVDRGLILAGGGALLNGIDKLIAEQTGLPVHVADDPLCAVAEGTGVVLHELNFL
                     RKMSGHEKS"
     gene            1501..2331
                     /gene="mreC"
                     /locus_tag="PKK36_05115"
     CDS             1501..2331
                     /gene="mreC"
                     /locus_tag="PKK36_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNR93974.1"
                     /translation="MLASILLVMLNLPTPVSRKVESSVREGISPLQQVFSGIGFRISE
                     ALIALRGLGGIVRENQELSAEMVRLQNETRLLRSLAAENADLRRQLGFAQRNISDYIP
                     CEVIGRDVSGWWQTIRLGKGVSENVGENRAVISAEGLVGKTIDVSLHTADVLLISDPA
                     CKVSARISRTGAFGIVTGRGVSPRGRAVCRMEFINKNINVLPGDEVLTSGLGGVYPQG
                     ILIGYVEKVYQDSHGLYQYAEIEPKADLGMITYVFVESRGDPVDEFMRERLQDRGGDR
                     "
     gene            2328..2831
                     /gene="mreD"
                     /locus_tag="PKK36_05120"
     CDS             2328..2831
                     /gene="mreD"
                     /locus_tag="PKK36_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNR93975.1"
                     /translation="MSAVAMVFLLVFGSVLQAVIPTGPAMGQVRLPVLLGLVIYYSLL
                     KPRRTALYSALLAGILQDALSMIPLGFSSFCFILTAWIISSWREEVFTRHWLTHMLMG
                     LAAAPANLLALYLLLTQARLLILPWAGLMLKLWGSVVTGVIFVPLVCRAAETLEVKLG
                     MLEARES"
     gene            2828..4645
                     /gene="mrdA"
                     /locus_tag="PKK36_05125"
     CDS             2828..4645
                     /gene="mrdA"
                     /locus_tag="PKK36_05125"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR93976.1"
                     /translation="MTVRPAIESGAFRIRILLVLMLVLCALLGVMLWKIQVAHGHDYV
                     QDLDRQSVRRIRLPAMRGTIVDRSGAALVENEPSYAVAVYIEELRQPGVNVVDAAEAL
                     IRRLGELLQLPVDVTRDDIANHYRKRLPLPFIVWKNLSEETLARWAELAAGIPGADIY
                     WEGLRHYPHGQTASHVLGYVGRASLTGREEEPYHYYLPEWEGKSGIEQSMDDVLRGRA
                     GGKLIRVDVAGYKHADLGGLDPEAGSDLMLTLDLHIQKLAEEALEGHNGAMVVMDPRN
                     GEILAMASAPAFDPNDFVPAISHELWAGLRENPAKPMVNRAVAGNYPPGSIFKPVVAM
                     AALESGKIRPGQRFNCPGYMMLGKARFNCWHKPGHGEIELRKAIEQSCNVYFYDLGLM
                     CGHEPIVHMAYALGLGRKTGVEMSYERAGLVPDDSWKRRTFSDGWRDGDTCNMSIGQG
                     ALLVSPLQMAVVASALANGGFVYAPHLVRGVRRNGDEKYRPVQPRLVNDLHWSSESLR
                     TVRGGMFDVVMAPTGTGRKAGVPGIEVAGKTGTAEYGPKGSGLKYGWMIAFAPYENPR
                     YAVAVVVEEAYSGGVTAAPLVSRLLGGIFRPAAGEGGAG"
     gene            4642..5757
                     /gene="rodA"
                     /locus_tag="PKK36_05130"
     CDS             4642..5757
                     /gene="rodA"
                     /locus_tag="PKK36_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNR93977.1"
                     /translation="MIDLTRIRHMLRRMDWALFCTVGALMVLGVLFIYSATWRGDGIP
                     MSPFYKKQIVWALIGMALFFLMALTDYHLLGENAWWLYATAMVLLVLVLFLGKEVYGA
                     HRWLSFFGVQVQPSELAKLAVIVALAKYLSRPGRDMESPQTTAAALLIMGLPFILILR
                     EPDLGTAAVLLPVVILMLYAVGVPLKTLIFLGVLGLLFLPVGWFGLDQYQKKRILVFL
                     DPTTDPLGAGWNKMQSEIAVGSGGLLGKGWLKGTQNVLGFLPRTVAPNDFIYSVVAEE
                     TGFVGSMAVLFLYVFVFAGCFRAALAARDAFGRLLCIGVGVMLYVHVMVNIAMTVGLM
                     PITGLPLPLMSYGGSFMVSIMAAMGIVQSVYVRRYRG"
     gene            5787..7349
                     /locus_tag="PKK36_05135"
     CDS             5787..7349
                     /locus_tag="PKK36_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNR93978.1"
                     /translation="MLFSKQITEQIIINCESLETRVAVLNEGNLEAFSVERPSEERVV
                     GSIFKGRIQNLEDGLQAAFVDIGLKKNAFIHYWDMIPEDMARLEAEEGVSRSRTTRRK
                     RYAAGEMAKIMPVGSDIIVQVTKDAIGTKGPRVTANLSIPGRYLVMLPGCELRGVSRK
                     IEDEKERVRLKKIMARLPVVPNNGFIIRTAGSGGRKTTFARDMRALVAIWQDIEEGIK
                     NKPAPCRLYQEPDLAERVVRDSLTSDIDRIIVDNREVYERLKIMVSKLSRIGRARLKL
                     YDGDAPIFEHFDVERQIESAFRRKVWLKSGGYIIFDETEALVAVDVNTGRHKGGKTQD
                     EAIHQVNIEAAQEIGRQLQLRNVGGLIVIDFIDMRQRRHQMTVYKELKNALKNDKART
                     NVLPISALGLLEMTRQRAEGSIRASNYAECPYCRGRGVVKNPLSMSVDIQRHIAAILR
                     RQRKDKHPLRITLHPRVLERMRSEDESILVDMEKKFETSLSFVADGNLHAEDFVIANA
                     DSGEELYRNSDH"
     gene            complement(7343..9178)
                     /locus_tag="PKK36_05140"
     CDS             complement(7343..9178)
                     /locus_tag="PKK36_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNR93979.1"
                     /translation="MSNACTSLFDAAGAGTAVIQQPAAAGRFRWLVFRQPVKCLTGHR
                     LDEVIPCLERIEEAAAEGLHAAGFISYEAASAFDAALPALSSDHTPLYWFGLYRSPTV
                     YECRMPAAAPVLPLPAVAGVPEMNRQDYRRRIEQIREYIAAGDCYQVNFTLRFQDEAR
                     DGLNSLFAGIASHGTGINTDTLAPCGACAACIHTGAHTVYSASPELFFSLDGDNIICR
                     PMKGTAGRGRTATEDRQRAAALRASEKDRAENIMIVDMVRNDLGRICKPGSINSGPLF
                     SIERYPSVFQMTSTVSGRTRAGLTEIFRALFPAASITGAPKIRAAEIIRELEISPRGI
                     YTGAIGHISPGRRARFNVAIRTLVHDHAGGRLLYGSGGGIVWDSTAENEHAETLIKTG
                     WLQPLDTEPFQLLETMRWHPRRGFFLLREHLDRMALSSQYFGFSFNRSEITADLNHAA
                     AALPCAAHRIRLLLDPDGTCRIEASVMDESAASRPWRVCLADTPTDRPDAFMLNKTTR
                     RAVYDRELARHPGMDDVILRNADGDITESCRANVIARINGRWITPPLEDGLLNGTYRE
                     YLLRRKIIHEERLSAEDFLHAAERRLINSVRRSIAVELQGDESNQ"
     gene            9281..10219
                     /gene="fmt"
                     /locus_tag="PKK36_05145"
     CDS             9281..10219
                     /gene="fmt"
                     /locus_tag="PKK36_05145"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNR93980.1"
                     /translation="MRIVFMGSDELACPSLRMLMARAQDELAAVITQPDRPKGRRRQP
                     APCPLKALAGQYAPLRILTPEKMNEPALLEELRALRPDIFVVVAYGGYIPQAVLKIAP
                     KGGINLHPSLLPKYRGAAPVQQAIACGETVSGVTILYVAEELDAGDIILQEAMPIEDE
                     DTGVTLFARAAEQGASLLGRALDLFERGEVSAVRQNPDQATYVTRLKKEDGRIVWEMP
                     ARQIRNRIRGFQPWPVCYFEWPKGSGLFVKVFKAALEQGTGQPGEVIDIQGEGPVVAA
                     GESALRLLEVQPGGRSVMSGKSFLCGHAMRPGVILG"
     gene            complement(10263..12218)
                     /locus_tag="PKK36_05150"
     CDS             complement(10263..12218)
                     /locus_tag="PKK36_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR93981.1"
                     /translation="MPCGAIRGFVRTGLLTACLAHALGCASVKPAAKPRFNRAEALAY
                     YSQGLIHQMQDRHAQATECFLKAIEHEPGRHSLYMIAAMSCIRQQQPDQAIAIIQQLC
                     DREPKAGDPRIWLAFMYQAAGRNNEARDTYRELIRRNPEDSRAYYELATLYNRTGAKD
                     EAVKTLERGAERAKDLSEILPMLAYLYTSRSAEAAPEEAALLREKAIHVLNRAAAEEP
                     DNAAILMQLGDLHILTDQLEKAIECYVMVDRLEPNNLQVKRKLALSFAANQQQGQAIQ
                     TMREISEQLPSNPEVYMFLGTLYENIGDRENAILNYRLIMKEEPDEAEVYLRLALLYL
                     DDEPAKSIQTLEDGLAANPENLRLTELMAYTFLAQSNYTRAVDFFGQAEKLQEAAQMQ
                     ADGLFHLHYAIALQFTGDYNAAGERLARAATANPELLGTYIALMFRRDEDAVRDAVER
                     TLHVTPVTGAIKADVLMYLGLLHHMRKDYHEAISHFELAEALHRETGVTNGSRAYFCF
                     WYGASCERAGQFDRAVKLLEQCIELDPSYADAYNYLAYIWAEKGIQLPRALQYIQTAL
                     ESDPDNAAYLDTLGWIYFKQGKYRAALEEIEKAIEFAPDEAEILDHLGDVYMKLGEKE
                     KAIEAWEKAAEFSDTPEKIRDKLNQHQ"
     gene            complement(12258..12347)
                     /locus_tag="PKK36_05155"
     CDS             complement(12258..12347)
                     /locus_tag="PKK36_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HNR93982.1"
                     /translation="MGSIKKQRIKKMAKHKRRKRLRADRHKKK"
     gene            complement(12404..13276)
                     /gene="hisG"
                     /locus_tag="PKK36_05160"
     CDS             complement(12404..13276)
                     /gene="hisG"
                     /locus_tag="PKK36_05160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNR93983.1"
                     /translation="MNKIVLGLPKGSLQETTFLLMKKAGFSIKLGSRSYVPVVDDPEI
                     EARLIRAQEISRYVEHGMLDVGMTGHDWILENGSDVVEVTDLVYAKQGMRPVRWVLAV
                     PNDSPIQSVKDLQGKRIATEAVGLTKRYLDKNKVKAEVEFSWGATEVKAPELVDAIVE
                     ITETGSSLRANNLRIVETVQESTTRLIANKAAWADKWKKQKISQLALLLNGALAAESR
                     VLLKMNVNRDNLDTVTRLLPSLHAPTVSALSSDGWCSVESVVEESFVREILPQLKAAG
                     AEGIIEIGLNKVIP"
     gene            13416..13491
                     /locus_tag="PKK36_05165"
     tRNA            13416..13491
                     /locus_tag="PKK36_05165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13449..13451,aa:Val,seq:tac)
     gene            14194..14874
                     /locus_tag="PKK36_05170"
     CDS             14194..14874
                     /locus_tag="PKK36_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93984.1"
                     /translation="MKKVVISLLVIGVLVTGVYGSSEKSTELKLGGEAIFPGQDDDWD
                     KGYGASARVIFWRTSELGLALSIGVQKWDVNDEMYTYSEYLGSGIGYGYAGGLEGDAT
                     MVPVGASGLYIIPVGKNASLTLEGGLRYVIMNSNVKYVEAEALADTYGNVLGASYSYD
                     VDFDNGIVGIIGADFDIELSPGFRLFAGAGYQIDLLKGDTEADGVDIDYENELKGAFI
                     RAGLAWDL"
     gene            15076..15828
                     /locus_tag="PKK36_05175"
     CDS             15076..15828
                     /locus_tag="PKK36_05175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR93985.1"
                     /translation="MNNNEPKGITFQPDIYPKVLSLLPPAPARVLDVGAGEGFFCGQL
                     RDRGYQVEACDFLQENFKLKDVLFHKGDLNCGVPLPDNQFDVVVSIEVIEHIENHVRF
                     ISELMRVLRPGGMIILTTPNILSLPSRWHFFLYGYTDCAPRPLDPTLDAYYMQHINPI
                     GMDQIQFLVERFGGEISDLSTNRIRRSAWLPYLLLKPILTLWLRGKLLRKEYKSFQPL
                     YSRNIQWMLHRANLMGRITIVVGRKSASRSQT"
     gene            16369..16881
                     /locus_tag="PKK36_05180"
     CDS             16369..16881
                     /locus_tag="PKK36_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNR93986.1"
                     /translation="MGVRGIQIVGMDVKELLLILNKALSDEWLAHYQYWVGSKVVAGP
                     MKDAVITELVQHATEEMAHAELLVTRIIQLGGTPVLTPAKWFELTNCAYEPPEDPFVQ
                     VVLEQNIRGEQCAISVYDKLMKLTKDADPVTYNIALQIIQQEVEHEEDLQALLEDLSL
                     MLKHYGGKKK"
     gene            complement(16885..17331)
                     /locus_tag="PKK36_05185"
     CDS             complement(16885..17331)
                     /locus_tag="PKK36_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNR93987.1"
                     /translation="MKTILVAIDFSDATPPAINHAANLAAQFKSKVWLIHVAQPEPDF
                     VGYKAGPQHVRDQVAHEIHREHERLQILEQQMREKGLDASALLIQGPTQEKILQEIER
                     LKVDLVIMGSHGHGAMRHLLVGSVTEGVLRKAPCPVLIIPCHHNAH"
     gene            17415..>17754
                     /locus_tag="PKK36_05190"
     CDS             17415..>17754
                     /locus_tag="PKK36_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNR93988.1"
                     /translation="MIMFDSLTQLANYGRRLAQQGLCAGAGGNISYRDGNVIWVKPSG
                     FSMEDLKVSLFAGLNLATGKQLRGDYRASSEAPMHLEIYRRCPAVNAIFHTHPPWLCG
                     VISSNAEFKPM"
BASE COUNT         3563 a         4897 c         5466 g         3828 t
ORIGIN      
        1 cactatcacc gcctaaccag ttgatgctgt ggaattaata ctgggacaga agtgcaaaaa
       61 cacccaaaaa tggctcaaac ggccgaaaaa actgcaaaat cgcaaaaaaa gcgaaaattt
      121 cgaaaaacgg gggaaaacgg ggccaaacag cggccgggat aaatagtact aaaatagtac
      181 tatatagata aaggcggtcg gcgaagccgg cgcgggcgga aaatgcgcgg ccggtccgtc
      241 acccggcgcg gacggttcaa cgtttcatgt taaatggctt ggctttatgc ttgacgggga
      301 agggggcatg tcgcaaagta cacccgattt ttcagcgaaa agcggaattt ttttaaggaa
      361 cggtttttat gttaagtcaa ctctttggct tattctcaaa cgatattggc attgatctgg
      421 gcacggccaa cacactggtc tatgtgcgcg atcgcggtat tgttctgcgc gaaccctcgg
      481 tggtggccat tcagtccggc accaaccggg tgcttgccgt tggagaggaa gccaagagga
      541 tgctgggccg gacacccggc agtattgtcg caatccgccc gatgaaagcg ggtgtgattg
      601 cagattttga aattacggag gccatgctgc ggtacttcat acggaaagtg cataaccgcg
      661 gcaaactggt caggccgcgt gtgatcgtgg ccgttccaag tggtattacg gaggtggaaa
      721 agcgtgcggt gaaggattcc gccgcccatg ccggggcccg cgaggtgtac ctgattgagg
      781 aacccatggc cgcggcaatc ggggtcggtc ttccggttca ggaaccggcg ggcaatatga
      841 tcgttgatat cggcggcggc acgacggaag tggctttgat ctccctcgcg ggcattgtct
      901 tcagccgaag cgtgcgggtg ggcggagacc aaatggacga gtccatcgtc cagcacatga
      961 agcgggtgta taatttgatg attggcgaac ggacggccga ggaaataaag atttccattg
     1021 gatcggccta tccgatgaag gaagaaacca ccatggaagt caagggccgc gacctggttg
     1081 caggcctgcc caaaaccctg acggtgacct cggaggaaat ccgggaggcc ctgcatgagc
     1141 cggtggccac cattgtggaa tccatccgga tcacgctgga gcgctgtccg cccgaactct
     1201 cggcggacct ggtggatcgc ggactgattc tggccggcgg cggcgcgctg ctgaacggaa
     1261 tcgacaagct cattgcggaa cagacgggcc ttcccgtgca tgtggcggat gatccgctgt
     1321 gcgccgtggc cgaaggcacc ggcgtggttc tgcacgagct caatttcctt cgcaaaatgt
     1381 cgggacacga gaagtcctga cgcccgccgt atctgaatgg caccccgtcc ggcgcacgcc
     1441 ggttccctcc gcacagacgg agtttggatt ttgcgggaca ggaagtttat tttattgctg
     1501 atgctggcca gtattctgct ggtcatgctg aatctcccaa cgccggtttc gcgcaaggtg
     1561 gagtcttccg tgcgcgaggg gatcagtccc ctgcagcagg tgttttccgg gatcggtttc
     1621 cgcatcagcg aggcgctcat tgcgctgcgc ggactgggcg gcattgtgcg cgaaaaccag
     1681 gagctgtccg ccgaaatggt gcgcctgcag aacgaaaccc gcctgctgcg gagcctcgcg
     1741 gccgaaaacg ccgacctccg ccgccagctc ggcttcgccc agcgcaacat cagcgattat
     1801 atcccctgcg aggtgatcgg ccgcgatgtc agcggctggt ggcagaccat ccggctgggc
     1861 aagggcgtca gcgaaaatgt gggggagaac cgcgccgtga tcagcgccga ggggctggtg
     1921 ggcaagacga ttgatgtttc tcttcacacg gcggatgtgc ttctgatttc cgatcccgca
     1981 tgcaaggttt ccgcgcgcat atcccggacg ggggcctttg gaattgtcac gggccgcggc
     2041 gtgtctccgc gcggccgcgc cgtctgccgc atggaattca tcaacaagaa catcaatgtg
     2101 ctgccgggcg acgaggtgct cacctccggc cttggcggcg tctatcctca gggcattctg
     2161 atcggctatg tggagaaggt ctatcaggac agccacgggc tctatcagta tgccgaaatc
     2221 gagccgaagg cggacctcgg catgatcact tatgtctttg tggagtcgcg cggcgatccg
     2281 gtggacgaat tcatgcggga gcgcctgcag gaccggggag gcgaccggtg agcgcggtgg
     2341 ccatggtctt cctgctggtc ttcgggtcgg tgctgcaggc cgtcatcccg acgggccccg
     2401 cgatgggcca ggtgcgcctg ccggtgctgc tcgggctggt gatctattac agtctgctga
     2461 aaccccgccg caccgcgctg tattcggccc tgctggccgg cattctccag gatgccctga
     2521 gcatgatccc cctcggattt tcaagtttct gcttcattct gacggcgtgg atcatcagca
     2581 gctggcgcga ggaggttttc acgcgccact ggctgacgca catgctgatg ggcctggcgg
     2641 ccgctccggc caatctgctg gcgctgtatc tgctgctgac tcaggcccgg cttctgattc
     2701 tcccctgggc cggactgatg ctcaagctgt ggggatccgt tgtcaccgga gtgatctttg
     2761 ttccgctggt gtgccgcgcg gccgaaacgc tggaagtcaa gctgggcatg ctggaggcga
     2821 gggagtcatg acggtccggc ccgcaataga atccggcgct ttccgcatcc ggatcctgct
     2881 tgtgctgatg ctggtgctgt gcgcgctgct cggcgtgatg ctctggaaaa tccaggtggc
     2941 gcacggccat gactatgtgc aggacctgga ccgccagagc gtgcggcgaa tccgcctgcc
     3001 cgccatgcgc ggaaccatcg tggaccgcag cggcgctgcg ctggtcgaaa atgagccgtc
     3061 ctatgccgtc gccgtctaca tcgaggagct ccggcagccc ggcgtgaacg tggtggacgc
     3121 ggcggaggcc ctcattcgcc gcctcggcga gctgctgcag ctcccggtcg atgtaacgag
     3181 ggacgacatc gcaaaccact accgcaaaag actgccgctg cccttcattg tctggaagaa
     3241 tctcagtgaa gaaaccctcg cgcgctgggc cgaactcgcg gccggcatcc ccggcgctga
     3301 tatttactgg gagggacttc ggcattatcc tcatggccaa accgcaagcc atgtgctggg
     3361 ctacgtcggc cgtgcaagcc tgaccggccg cgaggaggag ccctatcact actatctccc
     3421 cgaatgggag ggcaagtccg gcatagaaca gagcatggat gatgtcctgc gcggccgcgc
     3481 cggaggcaaa ctgatccgcg tggatgttgc cggctacaag cacgccgatc tcggcgggct
     3541 tgatccggag gccggttccg atctgatgct gacgctggac ctgcatattc agaagctggc
     3601 ggaagaagcg ctggaggggc acaacggggc catggtggtg atggatccgc gcaacggaga
     3661 aattctcgcc atggccagcg cgccggcctt tgatccgaac gatttcgtgc cggccatctc
     3721 gcatgaactc tgggccgggc tcagggaaaa ccccgccaag cccatggtga accgcgcggt
     3781 ggcgggcaac tatccgccgg gaagcatctt caagccggtg gtggccatgg ccgcgctgga
     3841 aagcggaaaa atccgtcccg gacagcggtt caactgcccg ggatatatga tgctgggaaa
     3901 agcccggttc aactgctggc acaaaccggg ccacggggaa attgaactca gaaaagccat
     3961 cgagcagtcc tgcaatgtct atttctatga tctgggcctg atgtgcggtc atgagcccat
     4021 cgtgcacatg gcctatgcgc tcggcctcgg ccgtaaaacc ggtgtcgaaa tgtcctatga
     4081 acgcgccggg cttgtgccgg atgactcatg gaaacgacgc acgttcagcg acggctggcg
     4141 cgacggcgac acctgcaaca tgtccatcgg ccagggcgcg ctgctggttt ccccgctgca
     4201 aatggccgtg gtggcctccg ccctggccaa cgggggtttt gtctatgccc cgcacctcgt
     4261 gcgcggcgta cgccggaacg gggacgagaa atatcggccc gttcagcccc ggctggtcaa
     4321 tgatctgcac tggagttccg aatcccttcg gaccgtgcgc gggggcatgt ttgatgtggt
     4381 catggccccg accggaaccg ggcggaaggc cggcgtcccc ggaatcgaag tggccggcaa
     4441 aaccggcacc gcggaatacg gaccgaaggg gtccggcctg aaatacggat ggatgatcgc
     4501 cttcgccccc tacgaaaatc cgcgctatgc ggtggcggtt gtcgtggaag aggcgtacag
     4561 cggcggcgtg acggccgcgc cgcttgtcag ccggctgctg ggtggaattt tccggccggc
     4621 cgccggggaa gggggcgccg gatgatcgac ctgacccgga ttcggcatat gctccgccgc
     4681 atggactggg ctctcttctg cacggtcggc gccctgatgg tgctcggtgt gcttttcatc
     4741 tacagcgcca cctggcgcgg agacggcatt cccatgagcc cgttctacaa aaaacagatc
     4801 gtatgggcgc tgatcgggat ggccctgttc ttcctgatgg cgctgaccga ttatcacctg
     4861 ctgggcgaga acgcctggtg gctgtatgcg acggcaatgg tgctgctggt gctggtgctg
     4921 tttctcggca aggaggtgta tggcgctcat cgctggctca gctttttcgg agtccaggtg
     4981 cagccgtccg aactggccaa gctggcggtg attgtcgcac tggccaagta tctgagccgt
     5041 ccgggaaggg atatggagtc cccgcaaacc acggcggccg ccctgctcat catggggctc
     5101 cccttcatcc tgatcctgcg ggaaccggat ctgggcaccg cggcggttct gctcccggtt
     5161 gtgatcctga tgctgtatgc cgtcggagtt cccctgaaaa cgctgatttt tctgggcgtg
     5221 ctcggactgc tgtttcttcc ggtcggatgg ttcggcctgg atcagtacca gaagaagagg
     5281 atactggttt ttctggatcc caccaccgat ccgctggggg cgggctggaa caagatgcag
     5341 tcggagattg ccgtgggctc gggcgggctg ctgggcaagg gctggctcaa gggcacgcag
     5401 aatgtgctgg gattcctgcc tcgcaccgtg gcgccgaacg atttcatcta ttccgtggtg
     5461 gcggaggaaa ccggatttgt cggatccatg gccgttttgt ttttgtatgt gtttgtgttt
     5521 gccggatgct tccgggcggc gctggcggcc cgtgatgcgt tcggccggtt gctgtgcatt
     5581 ggagtgggtg tgatgctcta cgtgcacgtg atggtgaata tcgcaatgac cgtggggctg
     5641 atgcccatca ccggtcttcc cctcccgctg atgagctacg gggggtcgtt catggtcagc
     5701 atcatggcgg cgatgggcat tgtgcagagt gtttatgtac gcaggtaccg gggatagccc
     5761 ggatttttca acaagaggag aacaacatgc ttttttcaaa gcagataacg gaacagatta
     5821 ttatcaactg tgaaagcctt gagacgaggg tggccgtgct gaacgaaggg aatctggagg
     5881 cgttcagtgt cgagcgtcct tccgaggagc gcgtggtcgg cagtatcttc aagggccgga
     5941 tacagaacct ggaagacgga ctgcaggccg cgtttgtgga catcggcctg aagaagaacg
     6001 cgttcatcca ttactgggac atgattcccg aagacatggc gcgcctggag gcggaggaag
     6061 gcgtcagccg gtcgcgaacc acgcgccgca agcgctatgc cgccggcgaa atggccaaaa
     6121 tcatgcccgt cggaagcgac attatcgtgc aggtcaccaa ggatgccatc ggcaccaagg
     6181 gcccccgcgt cacggccaat ctcagcattc ccggccgcta tctcgtgatg ctgcccggct
     6241 gtgagctgcg cggcgtgtcg cgcaagatcg aagacgaaaa ggagcgcgtc cgtctcaaga
     6301 agatcatggc ccggctgccc gtggttccca acaacggatt catcattcgc accgccggtt
     6361 ccggcggccg caaaaccaca ttcgcgcgcg acatgcgcgc gctggtcgcc atctggcagg
     6421 atattgagga ggggatcaag aacaagcccg cgccctgccg gctgtatcag gagcccgatc
     6481 tggccgagcg tgtcgtgcgg gattccctga ccagcgacat tgaccgcatc atcgtggaca
     6541 accgcgaggt ctatgagcgc ctgaaaatca tggtctccaa gctttcgcgc atcggccgcg
     6601 ccaggctgaa gctctatgac ggcgacgcgc cgatctttga gcacttcgat gtcgagcggc
     6661 agatcgaaag cgccttccgc cgcaaggtgt ggctgaaatc cggcggctat atcattttcg
     6721 acgagaccga agcgctcgtg gccgtggacg tgaataccgg ccggcacaag ggcggcaaga
     6781 cgcaggacga ggcgatccac caggtgaaca ttgaggccgc gcaggaaatc ggccgccagc
     6841 ttcagctgcg gaatgtcggc ggactgattg tcatcgactt cattgacatg cgccagcggc
     6901 ggcatcagat gaccgtctac aaggagctca agaacgcgct caaaaacgac aaggcccgca
     6961 ccaatgtgct gccgatctcg gccctgggcc tgctggaaat gacgcgtcag cgcgccgagg
     7021 gcagcattcg cgcctcgaat tatgccgaat gcccgtactg ccgcggccgc ggcgtggtga
     7081 agaatccgct gagcatgagc gtggatatcc agcggcacat tgccgccatc ctccgccgcc
     7141 agcggaaaga caagcatccg cttcgcatca ccctccaccc gcgggtgctc gaacggatgc
     7201 gcagcgagga tgaaagcatt ctggtcgaca tggaaaagaa gtttgaaact tcactgagct
     7261 ttgtcgcgga cggaaatctg cacgcggaag attttgtcat cgccaacgcg gattccggag
     7321 aagagcttta ccgcaattcc gatcactgat tcgactcgtc cccctgcagc tcaaccgcga
     7381 tgctgcggcg gaccgagttg atcagccggc gttcggctgc gtgcaggaaa tcttccgcgc
     7441 tcaaccgctc ttcatggatg atcttccgcc gaagcagata ttcacggtag gtgccgttca
     7501 gcaggccgtc ctcgagcgga ggggtgatcc agcggccgtt gatccgggca atcacattgg
     7561 cgcggcacga ttccgtgatg tccccgtccg cattccggag aatgacgtca tccatgccgg
     7621 ggtgcctcgc cagttcccgg tcatagaccg cgcgccgggt ggtcttgttc agcatgaagg
     7681 cgtcggggcg gtccgtcggc gtgtccgcca ggcagacgcg ccagggccgg gacgccgcag
     7741 attcgtccat gacggaggct tcaatgcgac aggtcccgtc ggggtccagc agcaggcgga
     7801 tccggtgagc ggcgcacggc agcgcggcgg cggcatggtt cagatcggcg gtgatttcac
     7861 tccggttgaa ggaaaagccg aaatactgcg aggaaagagc catacggtca agatgctccc
     7921 gcagcagaaa gaagccgcgc cgcggatgcc agcgcattgt ttcaagaagc tgaaagggtt
     7981 cagtgtccag aggctggagc cagcccgttt tgatcagggt ctcggcgtgc tcgttctcgg
     8041 ccgtggaatc ccagacgatg ccgcccccgg aaccatagag aaggcgaccg ccggcgtggt
     8101 cgtgcaccag agtgcggatg gccacattga accgtgcgcg gcggccggga gatatgtgcc
     8161 cgatggcccc ggtatagatt cccctgggtg aaatctccag ctcgcgtatg atttccgccg
     8221 cccggatctt gggtgcgccg gttatcgagg cggccgggaa aagtgcccgg aatatttccg
     8281 tcaggcctgc acgggtccgc ccgctgacgg ttgatgtcat ctgaaaaaca gacggatacc
     8341 gttcaatgct gaaaagcggg ccggaattta tgctcccggg cttacagatt cttccaagat
     8401 catttcgaac catgtccaca atcatgatgt tttctgcccg gtctttttct gatgcccgca
     8461 gggctgccgc tcgctgccgg tcttctgttg cggtccggcc gcgcccggcg gtgcccttca
     8521 tcggccggca gatgatgttg tcgccgtcca gcgagaaaaa aagctccggc gaagcggaat
     8581 agacggtgtg cgcgccggtg tgaatgcagg ccgcgcaggc gccgcacggc gccagcgtgt
     8641 ccgtgttgat gcctgttccg tgggatgcaa tgccggcaaa gagactgttc aggccgtcac
     8701 gcgcttcatc ctggaagcgg agagtgaagt tcacctgata gcagtctccc gccgcgatat
     8761 attctcttat ctgctctatg cggcgacggt aatcctgccg attcatttcc ggcactcccg
     8821 ccaccgcggg aagcggcagg accggcgcgg ccgccggcat tctgcactca taaacagtcg
     8881 gtgaccggta gagcccaaac cagtaaagag gcgtgtggtc agaggataag gccggcaggg
     8941 ccgcatcaaa agcggaggcc gcctcgtagc ttatgaagcc ggcggcatgc aggccttcgg
     9001 ccgccgcctc ttcaatccgc tccaggcagg gaatgacctc atccagccgg tgccccgtca
     9061 ggcatttcac gggctgcctg aaaacaagcc agcggaaccg tcccgcggcg gcgggctgct
     9121 gtatcacagc cgttccggcg ccggctgcgt caaacagcga tgtgcatgcg ttgctcatgt
     9181 tttatccaat gacagtgctt tccgaatatg cgatagtgct ccgtcggcga tgagtttgta
     9241 tcgcggtcaa ccgcggaagt ccagcaggtt ggagtatatc atgcggattg ttttcatggg
     9301 atcagatgaa ctggcgtgcc cgtcgctgcg catgctgatg gcgcgtgcgc aggacgagct
     9361 ggcggcggtg atcacgcagc cggaccggcc gaagggacgc cggcgtcagc cggccccctg
     9421 tcccctgaag gcgctggccg ggcagtatgc cccgcttcgg attctgactc cggagaaaat
     9481 gaacgaaccg gccctgctgg aagagctgag ggcgctgcgg ccggatattt ttgttgtggt
     9541 ggcctatggg ggctacatcc cgcaggccgt gctgaagatc gcgccgaagg gcggcatcaa
     9601 tctccatccc tcgctgctcc cgaaatatcg cggggcggct cctgtgcagc aggcgatcgc
     9661 ctgcggggaa acggtttcgg gcgtgaccat cctgtatgtg gcggaggaac tggatgcggg
     9721 cgatatcatc cttcaggaag ccatgccgat tgaggacgag gacaccggcg tgaccctgtt
     9781 cgcgcgggcc gccgaacagg gagcttccct gctgggccgc gcgctggatt tgtttgagcg
     9841 cggggaggtg agtgccgtca ggcagaatcc ggaccaggcg acctatgtga ccaggctcaa
     9901 gaaggaggat ggccgcatag tctgggagat gcctgcgcgc cagattcgca accggattcg
     9961 aggctttcag ccgtggccgg tgtgttattt tgagtggccg aagggatctg gtctttttgt
    10021 gaaagttttc aaagcggccc ttgaacaggg caccggccaa ccgggcgagg ttattgatat
    10081 tcagggggag ggcccggtag tggccgccgg tgaaagcgct ctccgcctgc tggaggtcca
    10141 gccgggaggc cggtccgtca tgagcggaaa atcctttctg tgcggacatg cgatgcggcc
    10201 cggtgtaata ctgggataat cagaagggcc ccgcaatcgg agggaagagc tgtgcggacc
    10261 cgttactgat gctggttcag cttgtcccgg attttttcag gggtatcgct gaattcggcc
    10321 gctttttccc aggcttcgat ggctttctct ttctcaccga gcttcatata cacgtcgcca
    10381 aggtgatcaa gaatttcagc ctcgtcgggc gcgaattcga tggctttctc gatctcctcg
    10441 agcgccgccc ggtattttcc ctgtttgaag tatatccatc cgagggtgtc gagataggcc
    10501 gcgttgtcgg ggtctgattc cagagcggtt tgaatatact gcagcgcccg gggcagctgg
    10561 atccctttct ccgcccagat gtaggcaagg tagttgtatg cgtcggcata agagggatcc
    10621 agttcgatgc actgctcaag cagtttcacg gcgcggtcga actggccggc tctttcgcag
    10681 ctggctccgt accagaagca gaagtaggcg cgcgatccgt tggtgacgcc ggtctcgcga
    10741 tgaagagcct ccgccagttc aaagtgcgaa atcgcctcat gataatcctt tcgcatatgg
    10801 tggagcagtc ccagatacat caggacatcc gccttgatcg cgccagtaac cggcgtgaca
    10861 tgcagtgtgc gctccaccgc gtcccggacg gcgtcttcgt cccggcggaa catcaatgca
    10921 atataggtgc cgagcagctc gggattcgca gtggccgccc gggccagtcg ctcaccggcg
    10981 gcgttgtagt cccctgtgaa ctgcagtgcg atcgcatagt gcagatggaa aagcccgtcg
    11041 gcctgcatct gcgctgcttc ctgcagcttc tccgcctgcc cgaagaaatc aacggcccgg
    11101 gtgtaattgg actgggcgag aaaggtgtag gccatgagct ccgtcagccg cagattctcc
    11161 ggattggccg ccagcccgtc ttcgagtgtc tgaatggatt ttgcgggctc gtcatcaaga
    11221 tagagcagcg ccaggcgcag gtaaacctcc gcctcgtcgg gttcttcttt catgatcagc
    11281 cggtagttga gaatggcgtt ctcccggtcc ccgatgtttt catacagggt tcccagaaac
    11341 atgtagactt ccggattgga cggcagctgc tccgagattt cccgcatcgt ttggatggcc
    11401 tggccctgct gctggttggc ggcaaaactc agagccagtt ttctcttcac ctgcaggttg
    11461 ttcggctcca ggcggtccac catgacatag cactcgattg ctttttcaag ctgatcggtc
    11521 aggatgtgca aatcgccgag ctgcatcagg attgcggcgt tatccggctc ttcggccgcc
    11581 gcccgattca ggacatgaat ggctttctcg cgcagcaggg cggcctcttc cggggccgct
    11641 tcggccgatc ttgaggtata cagataggcc agcatcggaa gaatttccga caggtccttt
    11701 gcccgttccg cgccccgctc aagggtcttg acggcctcgt ccttggcccc ggtccggttg
    11761 tagagcgtgg ccagctcgta ataggcccgg gaatcttccg gatttctgcg gatcagttcc
    11821 cggtaggtgt cgcgggcttc gttgttgcgt ccggccgcct gatacatgaa cgccagccag
    11881 attctgggat cgccagcctt aggttcgcgg tcgcacagct gctggatgat cgcgatggcc
    11941 tgatccggct gctgctgccg gatgcagctc atggccgcaa tcatatacag ggaatgacgc
    12001 ccgggctcgt gctcgatcgc cttcagaaag cactcggtcg cctgcgcgtg acggtcctgc
    12061 atctggtgaa tcagcccctg actgtaatat gcgagggcct ccgcgcggtt gaaacggggc
    12121 ttcgccgccg gttttacgga tgcacatccc aaagcatgcg caaggcatgc cgtcagcagg
    12181 cctgttcgca caaagccgcg tattgcaccg catggcataa tggcttcccc ggaacaggtt
    12241 gtcaggccgc aggcggccta tttcttcttg tgtctgtctg cgcggaggcg tttgcgccgc
    12301 ttgtgcttcg ccattttctt gatccgctgc tttttgatgc tgcccataag ggtgtgctcc
    12361 tgtattcctt caactcacga cctgagtcgt ggtcatagat taactatgga atgactttat
    12421 tcaatccaat ttcaataatt ccttcggcgc ccgccgcctt gagctgcggc aaaatttccc
    12481 ggacgaagga ttcctccacc accgactcca ccgaacacca gccgtcgctg ctgagcgcgc
    12541 tgaccgtggg ggcatgcagc gaggggagca gacgggtgac ggtgtccaga ttatccctgt
    12601 tcacgttcat tttcagaagc acccggctct cggccgccag cgcgccgttc agaagaagcg
    12661 ccagctggct gatcttctgc ttcttccatt tgtcggccca ggcggccttg ttggcaatca
    12721 ggcgggtggt ggattcctgc acggtttcca caatgcgcag gttgttggcc cggagggacg
    12781 agccggtttc ggtgatttcc acaatggcgt ccaccagctc gggagccttc acctcggtcg
    12841 cgccccagga gaattccacc tccgccttca ccttgttctt gtccagatag cgcttcgtca
    12901 ggccgaccgc ctcggttgca atgcgcttgc cctgcaggtc tttaaccgac tggatgggcg
    12961 agtcattggg cacggccagc acccagcgca cggggcgcat gccctgcttg gcatagacca
    13021 gatcggtcac ttccacgaca tcggatccgt tttccagaat ccagtcatgt ccggtcatgc
    13081 cgacatccag catgccgtgc tcgacatagc ggctgatttc ctgggcccgg atcagccgcg
    13141 cctcgatttc gggatcgtcc acgacgggaa cataggagcg ggaccccagc ttgatgctga
    13201 atccggcctt cttcatcaga aggaacgtgg tttcctgcag actccccttg ggaagtccca
    13261 gtacaatctt attcatggct gacctctgcg ttcatctagt taacaaagcg ccggatgata
    13321 gaccagattg cggattccgt ccatacccga agcgtctttt ttccggggtg ctgttgactt
    13381 gtacttaaaa gacgctattc tgcgccaaca tcgtcgggcg gttagctcaa ttggtagagc
    13441 gccacgttta caccgtggat gttacaggtt cgagtcctgt accgcccacc agccctctat
    13501 tcctaaaaaa agcctcaaaa atggcctaaa aacgtccaaa aatgcccaaa aacagccaaa
    13561 aaacaccaaa aaatgcaaaa aaatcagttt gaactacgaa aattacgaaa tgcgcgaaaa
    13621 tggagtgccg cattccattg gtatgtattt ctgccttcat gagttcctga ttcaattccc
    13681 ggaattttat atggaactca agaactcacg aaatcaactc cgatccgcgc aaattccttt
    13741 tgcttttgga ggaaagtttg gaaaatggct tgcaacaact ggacaactgt gcgaacaatg
    13801 agttccagaa aggcggggtg tttgaatgac cccggtttat caacggagtc tcgtaaagag
    13861 actgtaaaca aaatctgaat atcgcgtaat cgcaagattc gctcccacgg aaaaccacga
    13921 attttcagat gaaggaacga actggcgggg acgcgcccaa tcgggcgcgg ccctgtttca
    13981 tgttccttca ggggcttcgt ggtccctccg tggggcgtgg acgggtgtcc gcgcctcttt
    14041 tttttagtca ttcgggcttt caggctgaaa actaaaaggc ccaaaaataa gaacaggtac
    14101 gagtggatta taagcgaaga ctgatgttcg gcgaagatga agatgggcac atccaagccg
    14161 acacgacaaa acaatgccca cgaggagaca gcaatgaaga aggtagtgat tagcttactg
    14221 gtgattggag tgctggtgac gggtgtatat ggatcgtctg aaaaaagcac agaactcaaa
    14281 ctcggtggtg aagcaatatt tcccggacag gatgacgatt gggataaggg atatggtgca
    14341 tcagcccggg ttatattctg gcggacgagt gaactgggtc ttgctctctc tattggtgtt
    14401 caaaagtggg atgtgaacga cgaaatgtat acatacagcg aatatctcgg atctgggatt
    14461 ggttacggct atgctggggg cctggagggt gatgcgacaa tggtccccgt tggtgcatca
    14521 gggttgtaca taattcccgt cggaaaaaat gcatctctca ctctggaggg gggacttcgg
    14581 tatgtaatca tgaactctaa cgtgaagtat gtcgaagccg aagctcttgc agatacttat
    14641 ggtaacgtac ttggcgcttc ctattcgtat gatgtggact tcgataacgg aatcgttggt
    14701 ataattggtg ccgattttga catcgagttg agcccgggct tccggctatt cgccggggcc
    14761 ggttatcaaa ttgatttgct caaaggtgat actgaagctg atggcgttga cattgactat
    14821 gaaaatgaat tgaaaggtgc gtttatccgc gccggattag cttgggattt gtaacgagtt
    14881 ccaaaggcct gtgggggacg gctcacctaa agtgggttca ccgtcccctg acaggttgac
    14941 ccaaaaatcc gccgggcccc atctcggctg caagggccgg ccgctgaaga tgatattgag
    15001 cttataatct ttgagctaat gatgacatga gggaaatctc atgtttgcgg gcgcagattg
    15061 ttgcgagata ttcgtatgaa taataacgaa ccgaagggga tcacgtttca gccggatatt
    15121 tatccgaagg tgctgtcgtt actgccgccc gcgccagctc gtgtcctgga tgtgggtgcg
    15181 ggggaaggct ttttctgcgg gcagcttcgc gaccgtggct atcaagtaga ggcgtgtgac
    15241 tttcttcagg agaatttcaa gctgaaggat gttctgttcc acaaaggtga tttgaactgt
    15301 ggagttccat tgcccgataa tcaatttgat gtcgtcgttt ccattgaagt gattgaacac
    15361 atcgagaatc atgtccgctt catcagtgaa ttgatgcggg tactgaggcc gggaggaatg
    15421 atcatcctta cgacacccaa tattctgagt ttaccgtcac gttggcactt tttcctatat
    15481 gggtacacag actgtgcgcc gcggccactg gatcctacac ttgacgctta ttacatgcag
    15541 cacatcaacc ccatagggat ggatcaaatt caatttcttg ttgagcggtt tggcggagaa
    15601 atctcggact tgtccacgaa tcgcattcga cgaagcgcgt ggcttccgta tcttctcctc
    15661 aaaccgattc tcaccttgtg gctgcggggg aaactgcttc gaaaagagta taaatccttt
    15721 cagcccctct acagtcgcaa cattcaatgg atgctgcacc gcgcgaactt aatgggtcga
    15781 attacaatcg ttgtgggacg gaagagtgca agtcggagtc agacctagac aaatagacag
    15841 agcagagggg tctgttcatg cgttgctgcc ttgcatttta catgccgtga tttctgggtt
    15901 tgcgattccg ggttgagtcc gggcgggata atcatttcct gatttcctga gttccagatt
    15961 gtatccgtat cctcccagaa tgtgcctccg atgcgtccgg ggaggtgctg acatcacgga
    16021 tgaacaagag gattccccgc ggatagcaaa gccgtcccac ggataacgga aaaggtgaag
    16081 tgcagaacat gtccgccaat gcctgatata tcacatagtg atatatcgct ggatttttga
    16141 tatcggacga tgggtgactt gttgtccaaa tgggtgttgg ctcggcaggg attgattttt
    16201 tgagaacagc cgggcagcag aacttttcgt gcttttcgca tgttttgtag tttgtgaagc
    16261 tccggattgc atgcgcggtt gtttccctgt tgcttgactt ccgtttctgt ctgcgtcaca
    16321 ctgtgttcag tttgaaacgt taaccacgta atcaaccgga ggtgtgttat gggcgttcgc
    16381 gggattcaga ttgtcggaat ggatgtcaaa gaactgctgc tgatcctgaa caaggcgctg
    16441 tccgacgagt ggctggcgca ttaccagtac tgggtgggct ccaaggtggt cgccggcccg
    16501 atgaaggatg ccgtgatcac cgaactggtg cagcacgcga ccgaggaaat ggcccatgcc
    16561 gaactgctgg tcacacgcat tatccagctt ggcgggacgc cggtgctgac gccggccaag
    16621 tggtttgaat tgaccaactg cgcttatgag ccgcccgagg atccttttgt gcaggtggtg
    16681 ctggagcaga acatccgggg cgaacagtgc gccatcagcg tctatgacaa gctgatgaag
    16741 ctcacgaagg acgccgatcc ggtgacctac aacatcgcgc tgcagattat ccagcaggaa
    16801 gtggagcacg aagaagacct gcaggctctt ctggaagatt tgtcgctgat gctcaagcac
    16861 tacggcggaa agaaaaaata gccgtcagtg cgcgttgtgg tggcagggga tgatcaggac
    16921 cgggcagggg gctttgcgca gaacgccctc ggtgacgctt cccaccagca gatgcctcat
    16981 cgctccgtgc ccgtgcgatc ccatgatgac gaggtcaact ttgagccgct cgatttcctg
    17041 caggattttt tcctgcgtcg ggccctgaat cagcaaggct gacgcgtcca gtcctttttc
    17101 gcgcatctgc tgttcaagaa tctgcagacg ttcatgctcc ctgtggattt cgtgcgcgac
    17161 ctggtcgcgc acatgctggg gtcccgcctt gtagcctacg aaatcgggtt cgggctgggc
    17221 gacgtggatc aaccagactt tgcttttgaa ctgcgcagca aggttggcgg catgattgat
    17281 ggcgggcgga gtggcatcgg agaaatctat ggcgaccagt attgtcttca cacaacctcc
    17341 tttttcagag tagtctgtct gcatattaac agaaatctgt tccggcaaca gcataaaatg
    17401 ccggccacga ttttatgatc atgttcgaca gtttgactca gttggcgaat tacggccgcc
    17461 gccttgcgca gcaggggctg tgcgcggggg ccgggggcaa catcagctac cgcgacggca
    17521 atgtcatctg ggtgaagcct tcgggttttt caatggagga tttgaaggtt tcactttttg
    17581 ccgggctgaa cctggcaacg ggaaaacagc ttcgcggcga ttaccgggcg tcctccgagg
    17641 cgccgatgca tttggaaatc tatcgccggt gccccgcggt gaacgcgatt ttccacacgc
    17701 atccgccgtg gctctgcgga gtcatttcca gcaatgcgga attcaagccc atgt
//
LOCUS       DAOQWR010000044        17562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3612_length_17562_cov_5.328726, whole genome shotgun sequence.
ACCESSION   DAOQWR010000044 DAOQWR010000000
VERSION     DAOQWR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_17562_cov_5.328726"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PKK36_05195"
     CDS             <1..1372
                     /locus_tag="PKK36_05195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR93989.1"
                     /translation="FYTMLNDLKSLKNPVIAHFHEWMSATAIPELRRRGNRAGIVFTT
                     HATMLGRYLAMNNPEFYTHMTGIDWQAQSHHFNIMGPVMIERAAAHGAHVFTTVSKVT
                     GRECEALLDRAPDVLLPNGLNLRRFVAMHEFQNLHRSYKEKIHQFVMAHFFPSYTFDL
                     DNTLYFFTAGRYEYRNKGFDMTLEAMARLNYRIKLSGLNRTVVFFLITRQAYKSASAE
                     VFRRQAMLEEMHGACESIKDSLGSRLLMAAAKGHIPEYETLVDETSRMRLRRIMHAWP
                     SSVLPPFVTHDLVNDDEVLQKIRACKLINRPDDPVKVIYYPEFVSTSDGLLGIEYDNF
                     VRGCHLGIFPSYYEPWGYTPLECVACGVPAVTSDLSGFGSYLNRCMPDWAERGLYVAP
                     RRDKTEDESVNELTGWLYNFAQMDRRERISLRNKVERSSDEFDWHNLGRNYTEAYETV
                     CRRAGF"
     gene            1425..2600
                     /locus_tag="PKK36_05200"
     CDS             1425..2600
                     /locus_tag="PKK36_05200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNR93990.1"
                     /translation="MKAIVIVLDSVGIGEAPDAADFGDAGAATLPHLAKAVGGIKAPT
                     LEAMGLGNIQKLFPGGKPIAGVNAVEKPIASFAAMEEVSVGKDTITGHWEFAGLEINP
                     GFTNFPLEYPSFPDDLLKAFEKQTGRGTIGNKGASGTAILDELGEEHMKTGKWIVYTS
                     ADSVFQIAAHNDVVPLKELYKACEIARKLCDPLRVGRIIARPFIGKPGAFERTHDRKD
                     YAFEPTEPTILERLKDAGVPVYAVGKIEDIYVHRGITESVHTGNNVTSQAQVEKFTKE
                     KKDGLIFANYIDFDMVYGHRRDSEGYAKSIEQTDEWLAGYLPMLTEDDMLIVTADHGN
                     DPTFKGTDHTREYVPLLVYRKGQPGRSLGIRKGFYDIAQTLADFFKISPMPRGKSWL"
     gene            2645..3958
                     /locus_tag="PKK36_05205"
     CDS             2645..3958
                     /locus_tag="PKK36_05205"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNR93991.1"
                     /translation="MVPQWIIEKKRDGEALSREEIEFFINGFTDGSIPDYQMAAMAMA
                     IYLKGMTFDEVAVLTETMMNSGDIVDTSAIERPKVDKHSTGGIGDKVSLILAPLVACC
                     GGGVPMISGRGLGITGGTLDKFEAIPGYRTDLSVEEFIGVVNKCGCSIIGQTGRLAPA
                     DKKLYALRDVTGTVPSIPLITASIMSKKMAAGLDALVLDVKWGKGAFMKTRENAKALA
                     LSMVEVGTRMGKGMAALITDMNQPLGRTAGNTVEIIETVKALSGEGAEDLMIVTMALS
                     AQMLTLTGLAPGENAAMLMLEEKIRSGEALEKFKEMVQLHGGDVSSLEDFRKLPTAKT
                     IQPMLSPKNGYIAAVDAEKIGKAVLLLGAGRMKTTDTVDHAVGATDLLKIGEQVSKGD
                     PLLTMHINSTNRLAEAEKLFMDAFIFSDAPVSPPPFINELILPEE"
     gene            3962..4804
                     /locus_tag="PKK36_05210"
     CDS             3962..4804
                     /locus_tag="PKK36_05210"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNR93992.1"
                     /translation="MTISYDVLERALQSIKQAWPEAKPSCGLILGSGWSDVAEAFTVK
                     STIAYEDIPGLGAPGVVGHSGRLIWAEHAGLETFVFQGRRHWYEGEGWTPVSLPIYVL
                     KKLGCTRLMVTNAAGGINSDFQVGDLMVITDHINAMCAHPLIGKANSIWGPRFPDQTY
                     LYNAKLRDQLALAARKCNIRLKQGVYLAASGPTYETPAEIRSYKTWGADAVGMSTVPE
                     AMLANAAGIKVVGLSCITNFAAGILDQPLSHKEVTEATRVAMPKMTSLVLQFWGEMAE
                     AGLD"
     gene            complement(4848..5264)
                     /locus_tag="PKK36_05215"
     CDS             complement(4848..5264)
                     /locus_tag="PKK36_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNR93993.1"
                     /translation="MVYRKNITVPTNGHRDIHDITSQVADAVAESGIRTGLVNVFNVG
                     STAVIGTIEFEPGLERDLPEMLDRLIPPSCEYGHEQTWHDGNGHSHLQAALMGPSLIV
                     PVENGGLVLGTWQQIFHLECDVKSRSRRIVVTVTGD"
     gene            complement(5342..5418)
                     /locus_tag="PKK36_05220"
     tRNA            complement(5342..5418)
                     /locus_tag="PKK36_05220"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5382..5384),aa:Met,seq:cat)
     gene            complement(5487..5987)
                     /locus_tag="PKK36_05225"
     CDS             complement(5487..5987)
                     /locus_tag="PKK36_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNR93994.1"
                     /translation="MGKNLNRQERQAIISAIQRDLITIAKESFPKQCSCGRSYANLDE
                     FIAGTQSVEDGRGLFIDEDYASGSVVDLLRNCACGSTLMVAFETRRDHSPSGDKCRTI
                     FGRLHEFFTGCGLDGKVVRNELLRTSQGENSPVLETAFAEHDMDGMERDFIRLQIRAL
                     RILMKK"
     gene            complement(6038..7567)
                     /locus_tag="PKK36_05230"
     CDS             complement(6038..7567)
                     /locus_tag="PKK36_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR93995.1"
                     /translation="MKQLCTRMTMALILALFTGTAKVKAAWTHDIGAVSLFAELGASK
                     PTGTNITVSQIEGSYLGNYYPDIYTNEFSGKTFVDKTAMATGSSLHATMVGKYFYGRS
                     SSISPNINHVDCYWEEDWRDAGFLSGGDGGGPAVETNRIQNHSWIGNSGSDSNYVRRL
                     DYAIDRDGFTCVAGLNNTNDAIPGLLGHTYNGIVVGRTDGSHSAGPTLFDEAGRVKPD
                     IVAPFTFTSYATPAVGSCAALLMETADKHALTSATMPQTIKALLLAGATKTEFGTNWN
                     RTASRPLDEHYGAGEINIYNSWHILTNGEQNASATSLVNHIGWDYSELPAAGTNWYFF
                     DSPDSHAPSRFSVVLTWHRKISDPPGGGFNPQAFLANLNLYLYEADGFATNDLVDMST
                     SQVDNVEHIFQGALPPGRYALKVTSDAEAEYGLAWHKAPAAIPEFTETSYHSNTGFVI
                     KVSTSPNVFYGIQATTNLMDGSSWTNLATQIPSTSLWTFVDAKATNLIYRYYKAFVDP
                     D"
     gene            7713..8300
                     /gene="lexA"
                     /locus_tag="PKK36_05235"
     CDS             7713..8300
                     /gene="lexA"
                     /locus_tag="PKK36_05235"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNR93996.1"
                     /translation="MPRKLNLENILKKLRVFIRVERRMPGYAEMLELFGYRSKNAVHN
                     LLCRLERLGYITKNSRGKPAWTDKLIGSIRLLGAVQAGFPSPAEEELVDTLSLDEFLV
                     RQPEATFMLTVSGDSMIEAGIHPGDLVLVERGTAPKKNDIVVAQVDGEWTLKYFGRDR
                     HGIYLDPANSRYQRIRPRNSLTVGGVVRAVVRKYH"
     gene            8313..9629
                     /locus_tag="PKK36_05240"
     CDS             8313..9629
                     /locus_tag="PKK36_05240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNR93997.1"
                     /translation="MSPQDQILVFGTFPGAIVHFDGDAFFASVEQAADPSLKGRPVVT
                     GRERGIIACASYEAKALGVRRGISLRDARKICPQLVVLPSDYETYSLYSRRMFDIARR
                     FTPIVEEYSIDEGFADIGGLRRVFHVSYQDIAKQIQNTVREELNLTVSIGLSLTKSLA
                     KLASDFRKPAGFTAVAGMHLHLFLQRIPLDNVWGFGANTVQLLRKHGLKTAYDFILKP
                     EAWAQKMLGKPGHEIWRELRGQTVWPVTTEEKSSYASISKCKTFSEPSADRDYICARL
                     VRNLESAFIKVRRHKLRVREITVALRKDDYCQEGLRTALNRPTSSTQEALPLVLDLFG
                     WLFRPDWKYRSTMVVLGRLEEDREEQYDLFEDRIRIEKLHKASAVMDEVNGQFGKHRL
                     HIGTGQFLEMPRPSRDRDILPWRKTDLLKGETDRRRLALPRLKITV"
     gene            complement(9696..9965)
                     /gene="rpsT"
                     /locus_tag="PKK36_05245"
     CDS             complement(9696..9965)
                     /gene="rpsT"
                     /locus_tag="PKK36_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNR93998.1"
                     /translation="MPNNKSAEKRLRQSVEARQRNQSVKTRVKNTRRKFFESFEVAEG
                     ADPQAVYKKYCSELDKAAKKGVISRNTAQRRKTRAANRIRVKKAS"
     gene            10130..11026
                     /locus_tag="PKK36_05250"
     CDS             10130..11026
                     /locus_tag="PKK36_05250"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNR93999.1"
                     /translation="METGRSQNDYQPPARETCRRARALYRTALQTAALKIHTDGKTLS
                     GCWTDRPDMAPSELLMLVREAVTRPDSRIIKQTGRITVTRAHLEGREVIIKRYDLDGP
                     LERIRYLFHPSRARRFWAAASTLRKLGIPTPQPLGFLERWERGIPASSCMITAFVPGT
                     DDASHWLGWTSGTRPDDIKRALAKDLARAIVTLYRSGIYHRDTKTSNLLLEHPLDPER
                     RNYLWLDLECAVCGVRPGRHQIIRNVVQVAGSLGKEATCAERELIIREFAAWFPWLMD
                     RQALRHIHCWTDRRMHKERRCC"
     gene            11017..11703
                     /locus_tag="PKK36_05255"
     CDS             11017..11703
                     /locus_tag="PKK36_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94000.1"
                     /translation="MLLKFLKFCIAVLLLPACAVVCLSLFHLILALQPESSRQIPPSF
                     WGLAIGFFLWLFLFIVCQKPVRTYVLGHELTHALWAAVTGARVSRLRVSGKGGSVQVS
                     KSTFLITLAPYFFPFYTFCVLLLYAVISAFHNQRIYEPFWLGLVGLTWAFHLTFTVHM
                     LLQHQPDIHENGRLFSCVIILLFNAAGLCVWIVCMSSVTFDQLLAIITADSRTMLNAG
                     LRLLQTAYSG"
     gene            complement(11691..12884)
                     /locus_tag="PKK36_05260"
     CDS             complement(11691..12884)
                     /locus_tag="PKK36_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR94001.1"
                     /translation="MTPEEKIAMLEKLGTDFNRVKDMDLLMERILRSARAFAGADAGS
                     IYLLDNHRLHFSYTQNSTLESKLQPDAKLIYDCFSLPVDRTSIAGYSACTKETINIPD
                     AYAIDPEAPYRFEQGFDVSSGYRTKSILALPLLAAEQQVLGVLQLINAVDREGRTIPF
                     SPEIQRQMEMFAGLAAAALERAVMTRSIILRMVKMSELRDPRETGAHVNRVAAYAVEL
                     YERWAIKHGLPESRIEEEKDVLRIAAMLHDVGKVAISDLILKKPGRLTPEEFDVMKTH
                     AEQGADIFIGHMTAYDRAAQEVAINHHQKWDGTGYPSFVDDQTSEARPRRGTGIPLFG
                     RIVGLADVYDALVSRRVYKEAWSEDDALKVIREESGKHFDPELVDIFLSIRDILAAIR
                     KRYPE"
     gene            complement(12969..14165)
                     /locus_tag="PKK36_05265"
     CDS             complement(12969..14165)
                     /locus_tag="PKK36_05265"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNR94002.1"
                     /translation="MEWRDTDESYRVPILSWCRHVDDKAMSQARDLARHPHVFHHIAL
                     MPDCHPGYGMPIGGVIACPDHVIPNAVGVDIGCGMGAVRTDCEAAGVTKGTIRDILES
                     LKRMIPLGEGHGHRVPQEWTELDEFEASGNWMDARARELAWRNLGTLGGGNHFIELQA
                     GDDGFLWMMLHSGSRNLGYRIAEWHHGIARSYAADRKIELPTMDLASLPAASQEGQNY
                     IRDMNFALAYARENRARMMKRFKDAVQEHLSPGFTLEINIHHNYAAQEDHFGRMVWVH
                     RKGATSAREGEWGIIPGSMGSSSCIVRGLGNADSFQSCSHGAGRRLGRKEACRKLDKD
                     ACDRAMRGIEFDGWSRISGRNRSAGEYDLGEAPAAYKDIHEVMESQSDLVETAVHLRP
                     LGVLKG"
     gene            complement(14169..15131)
                     /locus_tag="PKK36_05270"
     CDS             complement(14169..15131)
                     /locus_tag="PKK36_05270"
                     /inference="COORDINATES: protein motif:HMM:NF021832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HNR94003.1"
                     /translation="MKKVRTDADRIRAFAEYLSAEGRPAPFDFCSLNAGITYPAPGLD
                     GVLEYFFFCCAHQFGFWNLQNGRWISPMIARLDGRDLKGSDYVWTAATRALRKNPDIF
                     SPHALANLNGNELNDLFLDDGAANPLPMWPEHLELTRQYADWFTTRKLQPADILHRAA
                     AARRPLQMLLDLLAEVPGYAEDPLQKKAMLLAIVLENRPEHFLNVTDPDSARPIIDYH
                     LQRSALRTGLVVVEDPALRARLEAREEATAGEEALIRSVVYESIVALVRLSGLSVAAV
                     DWFFFKNRTGCPEMTEPQCAACPVRHICARSTKLFQPVFRTTYY"
     gene            complement(15112..16008)
                     /locus_tag="PKK36_05275"
     CDS             complement(15112..16008)
                     /locus_tag="PKK36_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR94004.1"
                     /translation="MLLFLLKTISRFVSWLRLESALRFGRGLGWFIGSVLRYHRADAA
                     DALRRAFPEKPAAEIAAIIASMYAHLGMNLIESLRLAGGRREQVEGMISVSGKEHIDQ
                     ALARGRGVLLLTAHLGNWDLLSIMSLIAKLPPLTIISKDVKNRPLNDFWMGMRKEYGL
                     RIVPAHNSYRACRAALKNNEMVGFILDQNMIAREGVFVEFFGRPACTTPGLAFMAAQS
                     GAPVVPVSIYRTGAGHHEIHVQPVIEPPLDREPETIRAATQQYTTILEEMIRERPEQW
                     IWIHRRWRTKPGPAAADEKSSD"
     gene            complement(16032..>17562)
                     /locus_tag="PKK36_05280"
     CDS             complement(16032..>17562)
                     /locus_tag="PKK36_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR94005.1"
                     /translation="NKIPIDVMIIDTDWRDGWGGYDWSKKYFPNPEKLLKKLHSMNVH
                     SSLNDHPGYGNYDALPENDSHIAVIEKKLGALPHQGQWACDWSSKKAVQVWKDDILGP
                     FFDQGMDFWWIDGWIKGPFGSTDSQYWANRVYFELAEERLGKRGMILARWGGVGSHRY
                     PVQFSGDTASEWGVLKHQIEFTARSGNLGAAYWSHDIGGFFGRQVDEELLIRWMQFGA
                     MSPILRTHSDHGDREPWKYSSRVMKLFRKQVHIRYALAPYFYTLAREAHDEGLPIARP
                     LYLDHSHNDGGALWRKHQYMLGGDLLVVPADEPVDRKTGMLRKRVYFPKGRWHALESD
                     EVVQGTQDRWINIPVDCIPTYVREGAIIPCQPVGEALGCRVPEEIHFDYYPDSLNPSS
                     YVLYEDDGETMDYREGRCARLTLKGCRKEDCICFSISAPAGTYRGMPKKRKYVVRFLL
                     NDGDAIRGVCAKTGKGACADIKHRIVRQNLAGTVNSGRRFCEVAVSSANKSVDICVQL
                     K"
BASE COUNT         3755 a         5223 c         4841 g         3743 t
ORIGIN      
        1 gttctacacg atgctgaacg acctcaagtc gctcaagaat cctgttattg cgcatttcca
       61 tgagtggatg tcggccacgg ccattcccga gctgcgccgc cgcggcaacc gcgcaggcat
      121 tgttttcacc acgcacgcga cgatgctcgg acgctacctt gcgatgaaca accctgagtt
      181 ctacacccac atgaccggca tcgactggca ggcgcagtcc catcatttca acatcatggg
      241 ccctgtcatg atcgagcgtg ccgccgcgca cggggcgcat gtcttcacga cggtcagcaa
      301 ggtgaccggc cgcgagtgcg aggcgctgct ggaccgcgcg ccggacgtgc tgctcccgaa
      361 cggcctgaac ctgcgccgct tcgtggcgat gcacgaattc cagaaccttc accgcagcta
      421 caaggagaag atccaccagt tcgttatggc gcacttcttc cccagctaca cgttcgatct
      481 cgacaacacg ctgtatttct tcaccgccgg ccgctacgaa taccgcaaca agggcttcga
      541 catgaccctc gaggcgatgg cccgcctgaa ttaccgcatc aagctcagcg gcctcaaccg
      601 caccgtcgtg tttttcctga tcacgcggca ggcctacaag tcggccagcg ccgaagtgtt
      661 ccgccgccag gcgatgctcg aggaaatgca cggcgcgtgc gaatccatca aggattcgct
      721 gggaagcagg cttctgatgg cggcggcaaa gggacatatt cccgaatacg aaacactggt
      781 ggacgagaca tcccgcatga ggctgcgccg catcatgcac gcgtggccgt ccagcgtcct
      841 gcccccgttt gtgacccatg accttgtgaa cgatgacgag gtgctgcaga aaatccgggc
      901 ctgcaaactg attaaccggc ccgatgatcc cgtgaaggtg atctactacc ctgaatttgt
      961 ttccaccagc gacggcctcc tcggcattga atacgacaac ttcgtgcgcg gctgccatct
     1021 gggaatattc ccgagctatt atgagccgtg gggttatacc ccgctcgagt gcgtggcctg
     1081 cggtgtgccc gccgtcacca gcgacctgag cggctttgga agctatctca accgctgcat
     1141 gccggactgg gccgagcgcg ggctttatgt ggccccgcgc cgcgataaaa ccgaagatga
     1201 atccgtcaac gagcttacgg gctggctcta caatttcgca cagatggacc gccgtgaacg
     1261 catatctctc cgcaacaagg tggagcgctc ctcggatgag ttcgactggc acaatctggg
     1321 caggaactac accgaggcct atgaaacagt ctgccggcga gcggggtttt gacaaattcg
     1381 cgcggggcgg ctatgttgac cggattaaaa caggagaaac gtgaatgaaa gcgattgtga
     1441 ttgttctgga ttcagtgggt atcggcgaag cgcctgacgc ggcggatttc ggtgatgccg
     1501 gcgccgccac ccttccccat ctggccaagg ctgtcggcgg cataaaggct cccacgcttg
     1561 aggccatggg tctgggcaac atccagaaac tttttcccgg gggcaagccc attgccggag
     1621 ttaatgccgt tgagaagccc atcgcatcct ttgccgccat ggaagaagtc tcggtcggca
     1681 aggacacgat cacgggccac tgggagtttg cgggtctgga aatcaatccg ggattcacca
     1741 atttcccact ggaataccca tccttccccg acgacctgct gaaggccttt gagaagcaga
     1801 ccggccgcgg aaccatcggg aacaagggag cgagcggaac cgccattctt gacgaactgg
     1861 gcgaagagca catgaagacg gggaagtgga ttgtgtacac cagtgccgac tccgtcttcc
     1921 agattgccgc gcacaatgac gttgttccgc tgaaggagct ttacaaagcc tgcgaaatcg
     1981 cccgcaagct ctgcgatccg ctgcgcgtcg gccgcatcat cgcgcggccg ttcatcggga
     2041 agccgggggc atttgaacgc acgcatgacc gcaaggatta tgccttcgag cccaccgagc
     2101 ccaccattct tgagcggctg aaggacgcag gggttccggt ttacgccgtg ggcaagatcg
     2161 aggacattta cgtccatcgc ggcatcaccg agtcagttca caccggcaac aacgtgacgt
     2221 cgcaggcaca ggttgagaaa ttcacaaagg agaagaagga tggcctgatc ttcgcgaact
     2281 acattgactt cgacatggta tacggccacc ggcgcgactc ggagggctac gccaaatcca
     2341 tagagcagac cgacgagtgg ctggcgggct atctgccgat gctcacggaa gacgacatgc
     2401 tgatcgtcac cgccgaccac ggaaacgatc cgaccttcaa gggaacggac cacacgcgcg
     2461 aatatgtgcc gctgctggtg tatcgcaaag gacagccggg acggtcgctg ggaattcgca
     2521 aaggcttcta cgacattgcg cagacgctgg cggacttttt caagatcagc ccgatgcccc
     2581 gcggaaaaag ctggctgtga tactccattg acacccgcgc gggaaacggc tactctgcgc
     2641 gcatatggta cctcaatgga ttattgaaaa gaaacgagac ggcgaggccc tttcacgcga
     2701 ggaaatagaa ttcttcatca acggcttcac cgacggaagc atccccgact atcagatggc
     2761 cgccatggcc atggcgattt atctcaaggg aatgactttc gacgaggtcg cggtcctgac
     2821 cgagaccatg atgaactccg gcgatattgt cgacacctcg gccatcgaac gcccgaaagt
     2881 ggacaagcac tccacgggcg gcataggcga caaggtgtct ctgatcctgg cccctcttgt
     2941 ggcatgctgc ggcggcggtg tccccatgat ttccggacgc ggactgggca tcaccggcgg
     3001 aacactggac aagttcgagg ccattccggg ctatcgcacc gatttgagcg tcgaggagtt
     3061 cattggcgtc gtcaacaagt gcggatgttc catcattggc cagaccggcc ggcttgctcc
     3121 ggccgacaag aagctctatg cgcttcgcga tgtcactggg accgttccct ccatccctct
     3181 catcacggcc agcatcatga gcaagaagat ggccgccggg ctggacgcgc tcgtgctgga
     3241 tgtgaaatgg ggcaagggcg cattcatgaa gacccgcgaa aatgccaagg cactcgccct
     3301 gagcatggtg gaagtgggca cccgcatggg caagggaatg gcagccctca tcaccgacat
     3361 gaaccagcct ctcggacgca cggccggaaa cacggttgaa atcatagaaa cggtcaaagc
     3421 gctctcgggc gaaggggcag aggatctgat gattgtgacc atggccctgt cggcgcaaat
     3481 gctgacgctg accggcctgg cgccgggtga aaatgccgcc atgctgatgc tggaggaaaa
     3541 gatccggtcc ggcgaggcac tggaaaagtt caaggaaatg gttcaactgc atggcgggga
     3601 cgtttcttcg ctggaagact tcagaaagct gccgacagcc aaaaccattc agcccatgct
     3661 gtcgccgaaa aacggttata tcgccgccgt ggatgccgag aagatcggca aggcggttct
     3721 gctgctcggc gcgggccgca tgaaaaccac cgatacggtg gaccatgcgg tcggagccac
     3781 ggacctgctt aagatcggcg agcaggtttc gaagggcgat cccctgctta caatgcacat
     3841 caacagcaca aaccggctgg cggaagcaga aaagcttttc atggatgcct ttatattttc
     3901 tgatgcgcct gtgtccccgc ccccttttat taacgaactc atactgccgg aggaatagac
     3961 catgaccatc agctacgacg tactggaacg ggcgcttcag agcatcaaac aggcatggcc
     4021 tgaagccaag ccatcctgcg ggctgattct gggatccggc tggagtgacg tggcagaggc
     4081 ctttaccgtg aaatccacca ttgcctatga agacattcca ggccttggcg cgcctggcgt
     4141 ggtcgggcat tccggccggc tcatctgggc tgaacacgcc ggactggaga cgttcgtgtt
     4201 ccagggccgc cgtcactggt atgaaggcga gggatggact ccggtttccc tgcccatcta
     4261 tgtcctgaaa aagctgggat gcacccgcct gatggttacc aatgccgcgg gcggcatcaa
     4321 ttccgatttc caggtcgggg atctgatggt catcacggat cacattaatg ccatgtgcgc
     4381 gcacccgctc atcgggaagg ccaactcaat ctgggggccg cggtttcccg atcagacgta
     4441 tctctataac gctaaactgc gcgaccagct ggcgctggcc gcgcgcaagt gcaatatccg
     4501 cctcaagcag ggtgtttatc tggctgcatc cggaccgacc tatgaaaccc ccgccgaaat
     4561 acgctcctat aagacgtggg gcgcggatgc ggtgggcatg tccaccgtgc cggaagccat
     4621 gctggcaaac gccgcgggca tcaaggtcgt ggggctgtcc tgcattacta attttgcagc
     4681 cggaatcctg gatcagccgc tttcgcacaa agaagtcact gaggccacgc gggtcgccat
     4741 gcctaaaatg acatccctcg tactgcagtt ctggggcgag atggcggaag ccggcctcga
     4801 ctgatccatg tcttttgagg ctgcgggagg ctgtttttgc ggagtttcta atctcccgtc
     4861 accgtcacga caatcctgcg gcttctggat ttgacgtcgc attccagatg gaaaatctgc
     4921 tgccatgtcc ccagcacgag cccgccgttc tccaccggga ctataagcga cgggcccatc
     4981 agggccgcct gaagatgtga atgtccgttg ccgtcatgcc aggtctgctc atgcccatat
     5041 tcgcaggatg gcggaatcag gcgatccagc atttcaggca gatcccgctc caagcccggt
     5101 tcgaattcaa tcgtgcctat caccgccgtg ctgcccacat tgaacacatt cacaagaccg
     5161 gtccgaatgc cggattctgc cacagcatcc gcaacctgtg acgtgatgtc atgtatgtcg
     5221 cgatgaccgt ttgtcgggac ggttatattt ttccggtaca ccatgacctg aagcctatgc
     5281 ggcaactcct ttgagaaaca agagcaaaat gaaaagaccc gcttccgcgg gccttcaaaa
     5341 ttggtagcgg gggttggatt tgaaccaacg accttcaggt tatgagcctg acgagctacc
     5401 gggctgctcc accccgcaat caaatatcac gggagaacat aatgcacaca cgctgagatg
     5461 caagcgaaaa aatatttatt cctcggctat ttcttcatca agatgcgaag tgcgcggatc
     5521 tgcagacgga tgaagtctct ttccatgccg tccatatcat gttccgcgaa ggcggtttcc
     5581 agcaccggac tgttttcccc ttgcgaggtc cttaaaagct cgttgcgaac aactttcccg
     5641 tccagcccgc agccggtgaa gaactcatgc agccgcccga aaatagtacg gcacttgtct
     5701 ccgctgggag agtgatcgcg cctggtctca aacgccacca tcagcgtgga tccgcaagca
     5761 cagttccgca acaggtccac aacgctccca ctggcatagt cttcatcaat aaaaagcccg
     5821 cggccatctt ccacgctttg ggtccccgcg ataaattcat ccagattcgc atagctcctg
     5881 ccgcaggagc attgcttggg aaaagactct ttcgcaatgg tgatcaggtc cctctgaatc
     5941 gccgagatga tcgcctgacg ctcctgtctg ttcaaattct tccccataag aatccatttc
     6001 ccccaatcgc gcttcccttg ccggttcctc ttccgcttca atccggatct acgaatgcct
     6061 tgtaataacg atatataaga ttcgtggctt ttgcatccac aaaggtccac aggctggtcg
     6121 acgggatctg cgtggccaga ttggtccagg atgatccgtc catcaggttg gtggtggctt
     6181 gaatcccgta aaagacattc ggcgacgtgc tgactttgat tacaaacccc gtgttggaat
     6241 gataagaagt ttcagtaaat tccggtatgg cggccggggc cttgtgccag gcgagaccgt
     6301 attccgcttc ggcatccgat gtgactttca gggcgtagcg gccgggcgga agggctccct
     6361 gaaaaatgtg ctccacattg tccacctggc tggtgctcat atccaccaga tcattcgtgg
     6421 cgaatccgtc tgcctcgtac aaataaaggt tgagattggc taaaaatgcc tgcgggttga
     6481 atccgccgcc ggggggatcg gagatctttc gatgccacgt cagcacgaca gaaaaccgcg
     6541 agggggcatg gctgtccggc gagtcaaaga aataccagtt ggttcccgca gcgggaagtt
     6601 cgctgtaatc ccatcctata tgattcacca gcgaggtggc actggcgttc tgttctccgt
     6661 tggtgagaat atgccagctg ttgtaaatgt tgatctctcc cgctccatag tgttcatcca
     6721 gcgggcgaga agcagttcga ttccagttcg tcccgaattc ggtcttggtc gcgccggcca
     6781 gcagaagagc cttgatggtt tgcggcatgg tcgcactggt caaggcatgc ttgtctgccg
     6841 tttccatcag gagcgcggcg cagctgccaa ccgccggtgt ggcatagctg gtgaatgtga
     6901 acggggccac aatatccggt ttcacccgcc cggcctcatc gaacagcgtg gggcccgcgc
     6961 tgtgactgcc atctgtcctc ccgacaacga ttccgttata ggtatgtccc agaaggccgg
     7021 ggatcgcatc attggtgttg ttgagtcccg ccacacaggt gaaaccatcc cggtcaatgg
     7081 cgtaatccag gcgccggaca tagttggagt cactgccgga atttccgatc cagctgtgat
     7141 tttgaatccg gttggtctct actgccgggc cgccgccatc tcccccggaa aggaacccgg
     7201 catctcgcca gtcctcctcc cagtagcagt ccacatgatt gatgttcggc gaaatgctgc
     7261 tgctgcgccc gtagaaatat ttcccgacca ttgtggcatg cagggaggag cctgttgcca
     7321 tagccgtctt gtcgacgaaa gtttttcccg aaaattcatt ggtgtagatg tccggatagt
     7381 aattgcccag gtaagatccc tcaatctgtg aaaccgtgat attcgtcccg gtgggttttg
     7441 aggcgcccag ctcagcaaac agggaaacgg cgccaatatc atgggtccag gccgctttga
     7501 cttttgccgt gcccgtaaac agcgccagaa tcagcgccat cgtcattcga gtgcacaact
     7561 gcttcatatt tcctctatat ccttaattat gatcccgata tcacggcagt tcaaggccgg
     7621 aagaggatta ttgcatgagt gggatatttt atattccccc cttgatattg aactatagtt
     7681 caattatatt ccggacagaa aaggaatcgg tcatgcccag aaaacttaat cttgagaata
     7741 tactgaaaaa attgcgggtg ttcatccgcg tggaaagacg gatgccgggt tatgccgaga
     7801 tgctggaact gttcggctat cgctccaaga atgccgttca caacctgctc tgcagactgg
     7861 agcggctcgg ctatatcacc aaaaacagca gaggcaaacc cgcatggacc gacaagctga
     7921 tcggatccat tcggctgctg ggcgccgtgc aggcgggatt cccctccccg gccgaagagg
     7981 aactggtgga cacactcagc ctggacgaat tcctggtgcg ccagccggag gccacgttta
     8041 tgctgaccgt atccggcgat tctatgattg aggcagggat tcatcccggt gacctcgtgc
     8101 tggttgaacg cggaaccgcc ccgaaaaaga atgacatcgt ggtcgcgcag gtggacggcg
     8161 agtggaccct caagtatttt ggacgcgacc ggcatggcat ctatctggac cccgccaaca
     8221 gccgatatca gcggattcgg ccccggaact ccctgaccgt tggaggggtg gtcagggccg
     8281 tggtccggaa atatcattga gaggccgagc ctatgtcccc gcaggatcag atactggttt
     8341 tcgggacttt ccccggggcc attgttcatt tcgatggcga cgcgttcttt gcctccgtgg
     8401 aacaggccgc cgatccttca ctgaagggcc ggccggtggt gaccggccgg gagcgcggca
     8461 tcatcgcctg cgccagctac gaggccaaag cccttggggt caggcgcggc atttcactcc
     8521 gggatgcccg aaagatctgc ccgcagcttg tggtgcttcc cagcgactat gagacctaca
     8581 gtctctattc ccgccgcatg tttgacattg cacgccggtt tacccccatc gtcgaagaat
     8641 attccattga cgagggcttt gcggacattg gggggctgcg gcgtgttttt cacgtttctt
     8701 atcaggacat tgcaaagcaa atccagaata ccgtgaggga agagttgaac ctgaccgttt
     8761 cgatcggcct gagtctcacc aaaagccttg ccaagctggc ctccgatttc aggaaaccgg
     8821 cagggtttac cgccgtggcc ggcatgcatc tgcatctgtt tctccagcgc ataccgctcg
     8881 ataacgtctg gggcttcggc gccaataccg ttcagctgct caggaagcac ggactgaaaa
     8941 ccgcatatga ttttatcctg aagccggaag catgggctca gaaaatgctg gggaagcccg
     9001 gccatgagat atggagggaa cttcggggac aaacggtctg gcctgtcacc acggaggaga
     9061 agtccagcta tgccagtatc agcaaatgca aaactttcag cgagccttca gcggatcgtg
     9121 attacatctg tgcccggctg gtgcgcaatc tggaatccgc ctttatcaaa gtccgccggc
     9181 ataaactcag ggtgagggaa atcaccgttg cactgcgtaa agacgattat tgtcaggagg
     9241 gactgaggac ggcgctgaac cgccccacgt cttccactca ggaggcactc cccctggtgc
     9301 tggatctgtt cggctggctg ttccggccgg attggaaata tcgcagcacc atggtggtgc
     9361 tcggcaggct cgaagaggat cgcgaagagc agtacgatct ttttgaagac cgcattcgga
     9421 ttgaaaaact tcacaaggct tcagcagtca tggatgaagt gaacgggcag ttcggcaagc
     9481 acagactgca tatcgggaca ggacagttcc ttgaaatgcc gcgtccctca cgggatcgcg
     9541 atattctgcc ctggcggaaa acggatctcc tgaaaggcga aacagaccgc cggcgactgg
     9601 cactcccccg actgaaaata acagtttgac cgcctgagca taaaaacagc aaagccggcc
     9661 ggagccggct ttgcccatta aagctgaaag aatcttcagg aggctttttt cacgcggatg
     9721 cggtttgccg cgcgggtttt acggcgctgg gccgtattcc gggaaatcac gcccttcttg
     9781 gcggccttgt ccagctctga acagtacttc ttgtaaacag cctgaggatc agcgccctcg
     9841 gcaacttcaa atgactcaaa aaacttgcgg cgggtgtttt tcacccgggt tttcacgctc
     9901 tggttgcgct ggcgcgcctc aacggactgc cgcaaacgct tttctgcact cttattgttc
     9961 ggcatctgac aaatctccta actgatctca cgcagcggcc atagaggccg cccttccctt
    10021 tgaaaactgt gtcatactat ccataagagg atcgaagtca accgcatttg caaaaaaata
    10081 ctcattccag atgttgcaag ccgaagccgc cttgtgcaga atccgtgcca tggaaacagg
    10141 cagaagccag aatgattatc agcccccggc ccgcgaaacc tgcaggcggg cacgggctct
    10201 ttatcgcacc gcccttcaaa ccgcagcgct gaaaatccac acggacggga aaaccctctc
    10261 cggctgctgg accgaccgcc cggacatggc cccttcagaa ctgctcatgc tggtccgaga
    10321 ggcggtgacc cggccggatt cccgcatcat caagcagacc gggcgcatta ccgtcactcg
    10381 cgcacacctg gagggccggg aagtcatcat caaacgctat gatcttgacg gaccgctgga
    10441 acggatcaga tatcttttcc atccttcgcg cgcgcgccgt ttctgggctg ccgccagcac
    10501 actccggaaa cttggaatcc ccacgccgca gccactgggc tttcttgaac gctgggagag
    10561 aggcattcct gcttccagct gtatgatcac cgcctttgtg cccggcaccg acgatgccag
    10621 ccactggctg ggatggactt cgggcacgag gccggacgat atcaagcggg cgctggcgaa
    10681 ggatctggcc cgagccattg taaccctcta ccggagcgga atttatcacc gcgacacaaa
    10741 aacctccaac ctcctgctcg aacatccgct ggatcccgag cggcgaaatt atctctggct
    10801 ggacctcgaa tgtgccgtct gcggtgtccg gcccggccgt caccagatca tccgcaatgt
    10861 cgtgcaggtt gcaggatcgc ttggtaaaga agccacctgc gccgagcgtg agctgatcat
    10921 tcgtgaattt gccgcctggt tcccctggct gatggaccgg caggcgcttc ggcacattca
    10981 ctgctggact gaccggcgga tgcacaagga acggagatgc tgctgaagtt tctgaagttt
    11041 tgcattgcgg ttctgcttct tcccgcctgc gccgttgtct gtctcagcct ctttcatctc
    11101 atcctcgcgc ttcagccgga gtcatcccgt cagattcctc cttctttctg ggggctcgcc
    11161 atcggcttct tcctgtggct ttttctcttc atcgtctgcc agaagcccgt ccggacctat
    11221 gtccttggcc atgaactgac ccatgccctc tgggctgcgg ttaccggcgc gcgtgtttcc
    11281 cgtctgcggg tgagcgggaa aggaggatcc gtccaggtca gcaaaagcac ctttctgatc
    11341 actctcgcgc cctatttttt ccccttctac actttctgtg ttttgctcct ctacgcagtt
    11401 atctccgcct ttcacaatca gcgcatatat gagcctttct ggctgggact ggtcggcctg
    11461 acctgggctt tccatctgac gttcaccgtc cacatgctcc tgcagcacca gccggatatt
    11521 cacgagaacg ggaggctctt ttcctgcgtc atcatcctgc tctttaatgc agccggcctg
    11581 tgcgtatgga ttgtgtgcat gtcctctgtc accttcgatc agcttctggc aattataaca
    11641 gccgacagcc ggaccatgct gaatgcgggg ctccgcctgc tgcagaccgc ttattcagga
    11701 tagcgcttcc ggatggccgc aaggatatcg cggattgaaa ggaagatatc aaccagttcc
    11761 ggatcgaagt gttttccaga ctcttcccgg ataaccttta aagcatcatc ttcagaccag
    11821 gcctccttgt agacccggcg tgaaaccagg gcatcataga catctgccag cccgacgatg
    11881 cgtccgaaaa gcggtattcc cgttccacgc cgaggtcgcg cctcgctggt ttgatcatcc
    11941 acaaaggatg gatatccggt tccatcccat ttctggtgat gattgatagc cacctcctga
    12001 gctgcacgat cgtaagcggt catatgacca atgaaaatat ccgccccctg ctccgcatgg
    12061 gtcttcatca catcgaactc ctccggcgtg agccttccgg gcttcttcag aatcaaatcc
    12121 gaaatggcca ccttgccgac atcgtgcagc atggcggcaa tgcgcaggac atccttttcc
    12181 tcctcaatcc tgctttccgg aagcccgtgc tttatcgccc agcgctcata gagttccact
    12241 gcataggccg ccacgcggtt cacatgcgct cccgtttcac gcggatcgcg cagttcggac
    12301 attttgacca tgcggagaat aatcgagcgc gtcatcaccg cgcgctccag cgcagcagcg
    12361 gccaggcccg caaacatctc catctgacgc tggatttccg gggagaaagg gatggttctg
    12421 ccctcccggt ccaccgcatt gatcagttga agcactccaa gcacctgctg ctctgccgcc
    12481 aggagcggca gggcaaggat agatttcgtg cggtaaccgg acgacacatc aaaaccctgt
    12541 tcaaaccgat agggcgcttc cggatcaatt gcataggcat cagggatgtt gatcgtctcc
    12601 ttcgtgcagg cggaatagcc ggcaatggag gttctgtcaa cgggaagtga aaaacaatca
    12661 tagatcagtt ttgcatcagg ctggagttta ctttccagcg ttgagttctg agtgtaactg
    12721 aaatgcaggc gatggttgtc cagcagataa attgatccgg catcggctcc ggcaaaagcg
    12781 cgggcgctgc gcagtatgcg ctccatgagg aggtccatat cctttacccg gttaaagtcc
    12841 gtcccgagtt tttccagcat cgcgattttt tcttccggcg tcatcttccc tccggcttcc
    12901 cccacaatat aaccatcccg cttaaaaaga taatgcaaac tttgccagat tttcttccga
    12961 atttccggtc atcccttcag cactccaagc gggcggaggt ggacagctgt ttccacaaga
    13021 tcggactggg actccatgac ctcatggata tccttgtagg cggcaggggc ttcgcccagg
    13081 tcatattctc cggcgctgcg gttccggccg ctaatgcggc tccagccgtc aaactcgatg
    13141 ccgcgcatgg cccggtcgca ggcatccttg tccagtttcc ggcaggcttc ttttcttcca
    13201 aggcgacggc cggcgccatg cgagcacgac tgaaacgagt ccgcatttcc gaggccccgg
    13261 acaatgcacg aactcgagcc catgctcccg ggaatgattc cccattctcc ttcgcgagca
    13321 gacgtggccc ccttgcggtg cacccacacc atgcggccga aatggtcttc ctgcgctgca
    13381 tagttgtgat ggatattgat ctccagggta aaaccaggag acaaatgctc ctgcacggca
    13441 tccttgaagc gcttcatcat ccgtgcacgg ttttcgcggg cgtaggccag cgcaaagttc
    13501 atgtcccgga tgtagttctg tccttcctgc gaggccgccg gaagcgaagc caaatccatt
    13561 gtcggcaatt cgattttcct gtctgcagca taacttctgg caatgccgtg atgccattca
    13621 gcaatccggt agcccagatt gcgcgagccg gaatgaagca tcatccacag gaatccatcg
    13681 tctccagcct gcagttcaat aaaatgattg ccccctccca gtgtgcccag gttccgccag
    13741 gccagttccc gcgcgcgggc gtccatccag ttccccgagg cctcgaattc gtccagctca
    13801 gtccattcct gaggcaccct gtggccgtgg ccctcgccca gcgggatcat ccgtttgagg
    13861 ctttccagta tgtcgcggat ggttcccttt gtgacccctg ccgcttcgca gtcagtccgg
    13921 acagcgccca ttccgcatcc gatgtccact cccaccgcat tgggaatcac atgatccggg
    13981 caggcaatca ccccgccgat cggcatgccg tagccgggat ggcagtccgg catcagggct
    14041 atgtgatgaa agacatgcgg atggcgcgcg agatcacgcg cctgactcat cgctttgtca
    14101 tccacgtgcc ggcaccagga tagaatcggc acgcgatacg attcatctgt gtcccgccat
    14161 tccatggctc aatagtacgt tgttcggaac accggctgga agagttttgt gctcctggcg
    14221 caaatatggc gcacagggca tgccgcgcat tgcggttctg tcatctccgg acagcctgtt
    14281 cggtttttga agaaaaacca gtccacggcc gccacagaga gcccggacaa gcgcaccagc
    14341 gcaactattg actcatacac cactgagcgg ataagagcct cctctcctgc agtcgcctct
    14401 tcgcgggctt cgagtctggc acgcagcgcc ggatcttcaa caaccacgag ccccgtcctc
    14461 agcgcgcttc gttgcaggtg ataatcaata atgggacggg ccgaatcggg gtcggtgaca
    14521 ttcaggaaat gctccggccg gttttcgaga acaattgcaa gcagcatggc ttttttctgg
    14581 agaggatcct cggcataacc gggcacttca gcaagcaaat ccagaagcat ctgcaggggc
    14641 ctgcgggccg cggcggcgcg gtgcagaata tctgcgggct gcagttttct ggttgtaaac
    14701 caatccgcat actgccgagt aagctccaaa tgttccggcc acatcggcag aggattcgcc
    14761 gcgccgtcat ccagaaacag atcattcagt tcattgccgt tcaagttcgc gagcgcatgg
    14821 ggactgaaga tatccgggtt tttccgtagt gcgcgcgtcg cggccgtcca gacataatct
    14881 gatcccttca gatcccggcc gtcaagccgg gcgatcattg gcgagatcca ccggccattc
    14941 tgaaggttcc agaatccaaa ctgatgcgcg cagcagaaaa agaaatattc aagcacgccg
    15001 tcaagtcccg gcgccggata ggtgatgccc gcattcaggc tgcagaaatc aaacggcgcg
    15061 gggcgaccct ctgcggaaag gtattccgca aacgccctga tcctgtctgc gtcagtccga
    15121 acttttttca tccgcggccg ccggtcctgg ttttgtgcgc cagcgtctgt gaatccatat
    15181 ccactgttca gggcgctccc gaatcatctc ctcaagaatg gtggtgtact gctgcgtggc
    15241 tgcccggatg gtctcaggct cgcggtccaa aggcggttca atgaccggct gcacatgaat
    15301 ttcgtgatga ccggctccgg tgcgatatat gctgaccggg acaacgggcg cgcccgattg
    15361 agcggccatg aaggccagcc cgggcgtcgt gcaggcaggc cggccgaaga attccacaaa
    15421 tacaccctcc cgggcgatca tgttctgatc aagaataaat ccgaccattt cattgttttt
    15481 cagggctgcg cggcaggccc ggtaggaatt gtgcgcaggc acgatgcgca ggccgtattc
    15541 cttgcgcatg cccatccaga agtcgttaag cggcctgttt ttcacatcct ttgaaatgat
    15601 ggtcagcggc ggaagttttg caatcagcga cataatgctc agcagatccc agttccccag
    15661 atgcgccgtg agcagcagta ccccccttcc ccgcgccagc gcctgatcaa tgtgttcctt
    15721 cccgctgacg gagatcatgc cctccacctg ctcccgtcgt cctccggcca gccgaagcga
    15781 ctcgatgagg ttcatgccga gatgggcata catcgaagcg atgatggcag caatttcagc
    15841 ggctggcttc tccgggaaag cgcggcgcag cgcatcagcc gcatctgcgc gatgatagcg
    15901 cagcaccgaa ccgatgaacc atcccagccc cctgccgaag cgaagcgccg actccagccg
    15961 gagccatgag acaaaacggc ttatggtctt cagtaaaaac aacagcatga taatgaaatc
    16021 ctgctgtcac cctacttcaa ttggacgcag atatccaccg atttattggc cgaggaaacg
    16081 gcgacttcgc agaaccggcg gcccgaattc acggttccgg ccaggttctg acgtacaatc
    16141 ctgtgtttaa tgtctgcaca ggcccctttg cctgtttttg cgcaaacacc ccttatcgca
    16201 tcaccgtcat tgagcaggaa gcggaccaca tatttccgct tcttcggcat gccccgatag
    16261 gtgccggccg gagccgatat ggaaaagcag atgcagtctt ccttccggca gcccttcaga
    16321 gtcagacggg cacagcggcc ctcccgataa tccatggtct cgccgtcatc ctcgtacagc
    16381 acatagctgg agggattcag tgaatccgga tagtagtcga aatggatttc ttccgggaca
    16441 cggcagccga gcgcctcacc cacgggctga cacggaatga tggcgccctc gcgcacatag
    16501 gtcgggatgc agtccacggg gatattgatc cagcggtcct gcgtgccctg caccacttca
    16561 tctgattcca gcgcatgcca gcggcctttc gggaaataga cccgcttgcg gagcatgccg
    16621 gtttttctat ccaccggctc atcggcgggc accaccagca gatcgccgcc cagcatgtac
    16681 tggtgcttcc gccaaagcgc gccgccatcg ttgtgactgt gatcaagata gagcgggcgt
    16741 gcaatcggga gcccctcgtc gtgagcctcg cgcgccagcg tgtaaaaata cggggccagc
    16801 gcatagcgga tgtgcacctg tttgcggaat agcttcatga cgcggctgct gtacttccac
    16861 ggctcacggt cgccgtggtc gctgtgtgtg cgcaggatcg ggctcattgc gccgaactgc
    16921 atccaccgga tcaggagctc ctcatccacc tgccgcccga agaatccacc gatatcatgc
    16981 gaccagtagg ccgcgcccaa attgccggac cgggctgtga attcgatctg gtgcttcaga
    17041 acgccccatt cggaggccgt gtcgccagag aactgcaccg gatagcggtg agacccgacg
    17101 ccgccccagc gggccagaat catgccgcgt tttccgagcc gctcctccgc cagttcgaaa
    17161 tagacccggt tggcccagta ctggctgtcg gtggaaccaa aggggccttt gatccagccg
    17221 tcaatccacc agaaatccat gccctggtca aagaacggac ccagaatgtc atccttccag
    17281 acctgaacgg ccttcttgct ggaccagtcg caggcccact ggccctgatg cggaagggcg
    17341 ccaagtttct tctcgatcac cgcgatatga ctgtcatttt ccggcagcgc atcgtagttg
    17401 ccataacccg gatggtcatt caggctggaa tgcacattca tgctgtgcag tttcttcaag
    17461 agcttctcgg ggttcgggaa atactttttc gaccagtcat aaccgcccca gccgtcgcgc
    17521 cagtctgtgt cgatgatcat aacgtctatg ggaattttgt tt
//
LOCUS       DAOQWR010000045        17405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3657_length_17405_cov_4.670490, whole genome shotgun sequence.
ACCESSION   DAOQWR010000045 DAOQWR010000000
VERSION     DAOQWR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17405
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3657_length_17405_cov_4.670490"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKK36_05285"
     CDS             <1..468
                     /locus_tag="PKK36_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNR94006.1"
                     /translation="GVRTKIPQTGIVTIGDDVEIGANTAIDRARFGVTRIGSGVKIDN
                     LVQVAHNVEIGDHAVVVAQVGISGSSSVGRHAVLAGQVGVVGHVHIGDGAIVGGKSVV
                     TKDIPPRTFVSGNPAGPHKEEQRLQALTRKLPEMKQKIADIEKRLQELEKKRS"
     gene            complement(469..2547)
                     /locus_tag="PKK36_05290"
     CDS             complement(469..2547)
                     /locus_tag="PKK36_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR94007.1"
                     /translation="MKRCLLTLTAALLPLCANAEDSPGNTPLNLGSITVTASKFDENP
                     QTLPLSLSVFTQDSLSRNHLHSMQDISSLTPGFSFYSSGSRRTALYYLRGIGSSGPTP
                     PAVGVFLDDVYLHKTGFSDLELHDVEQIEILRGPQSTLYGKNTQAGAVKIQTVAPGDV
                     LNGRIEQTAGSDSYFQTRASSGAATDDGKFSFRSSMYYRERDGYVENDFLDTRADNIT
                     QKGGMLSLRAVPNDTLDMRLTLLGDRDSDGGYALAPLADVERNPHHVNMNIPGSHERD
                     LSLIALNVKVTGERLALQSITSFTDWENHDVYDSDFSPADIMSMNDKDWLEAFTQELR
                     LSSGSRRDELRWMAGGYFSTSRGEDENTSSFGSDGAMYGAVPGFAEHAMQDLDAYTLA
                     AFGHIIQPVGEKASVRAGLRYDHDRLEQNKQITRRMGGVPIPGMSMASDDARTEKEWS
                     PEIALDYMWAGNLLSYARIARGWRAGGFNSPIPGADPDYDPEISWNYEAGIKAGGPAD
                     RFMLNLSVFYIRIEDQQLMQFAPNSVSYYFRNAGEAESRGLEAEFTARATDWLDIFAS
                     AGYADMEYTESPEVDGNKLPFAPAFNYAVAARCHFPVGGPWQFVSQAGVHGQSSTYWD
                     EANEVKTDGYSLVDVQAGFENGNWSLRLFAKNLLDEEYAGVVHAFPGSTALAQAGDPV
                     TYGITVSCSF"
     gene            2647..2997
                     /locus_tag="PKK36_05295"
     CDS             2647..2997
                     /locus_tag="PKK36_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94008.1"
                     /translation="MNENPSRCYVFCPHEGLQNAGVCAGGAERLREKAQLPRLFFLPV
                     VQRRALQSVPEPQKHFLPASLLQILQESAQTSENRKILILRPQKPKNCPKWLKNSQKQ
                     PKTAPKGLKRLKKR"
     gene            complement(3103..4989)
                     /locus_tag="PKK36_05300"
     CDS             complement(3103..4989)
                     /locus_tag="PKK36_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNR94009.1"
                     /translation="MPIISQIGRKAWKTRTLYALIYALLALGAATMLYPFLVMLSGST
                     KSAVDLGDLDIIPRFLRDDTMLYRKYIEGLFNESLDTMNMVYGTSYRSFEEVEPPENI
                     DFGQIELWTGFLSENPPPAYASACGFVQARISKTIPMNLRNFKKHLAGKYGKSAGDVN
                     RALGTDFIGWNAFFLIPENFMLRRDMPRTDPFSAELQEFKKSLDPALFYYFSADGFYR
                     NLFLKTQYSGDIAAYNKAHGTAFDSYDEITLSRTLPDGTAAEKADWENFVRHTLNLLW
                     VRADPAAVELYHDFLEAKYGKIKTLNRICGTAHRSFNDIPLVEEPPFEGIVLSDWESF
                     ITGWEDPDTGRLHIIPAEFLRVDTLEFRFRDWLKAETMEGFVPSKPFFDKTPIPQREL
                     HCAAFLNMKKDMAWEFATRNFKTVLEYIVLHGRGILNTAIYCALAVLCALIVNPLAAY
                     AMSRNRMPSTYKILLFLMATMAFPPMVTQIPVFLMLRKMQLLNTFAALVLPGLANGYA
                     IFLLKGFFDSLPRELYECAELDGANEWTMFWHITMSLSKPILSVIALQAFTMAYSNFM
                     FALLICQDEKMWTLMVWLYQLQQRSGQPVIYASLIIAAVPTFIIFAVCQKIIIRGIVV
                     PVEK"
     gene            complement(4992..7721)
                     /locus_tag="PKK36_05305"
     CDS             complement(4992..7721)
                     /locus_tag="PKK36_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR94010.1"
                     /translation="MNCKWRVFSLALVLCAACAVQGGWIEDRDGKTVIHVKLFDLPDP
                     SRTDTSTRADAAAVKEFVRRFPEIFAERCREKYQANPEKYGRHNWDHVEIELHRFSGI
                     QVEGVGSELLAVAGGVAPDVMYVNFRMSDTYIQQGFLYPMDKPEDGYLSAMTQEELDF
                     RVHPKIWPVIRRQGPGRNTQVWAMPYGGALGKVVIYRKDLFDAAGVPYPHHNWTWDDF
                     YSACTNIADPGRGIYGIHFSKGKHESWWWITFLWSAGGEIMTWDESSGAWTIDFDSPE
                     AAVALDFYNRLCTEEWTDAENRRRYGYAHRDADWGSTAAKWDRGEIAMMIAYIDEKVF
                     QRINPDITGMAPVPIGPGGERGAELNSMMMGLFAGIRDAAVRDAAWEYIRFYDSKDAA
                     RIKTRIMVEGGLGRFINPRYLEMFGYPELVRLAPRGWKECFDIALETGRPEPYGKNSN
                     AIYDLMTRPIETAEELALQGRLPAGRDERLALFQKLLRQSAQKAREEMLGTLPPATLR
                     IHRLTALLALAGICTAFVWGFIRIGRAFTPPKAERQHGESWNPRRTVWAWLLLMPALL
                     TIFLWQYLPLVRGSVMAFQDYQIMGRSSWVGLDNFGSVLWDTQWWAAVWNSVRYCLLV
                     IGLTFLPPVILAILLQEIPRGKLLFRTIYYLPAVMSGLVVILLWKTFYDPTERGLLNA
                     LMMRIPAAGYLAIGLFLFILACSFTRRLLYHGRRTWALLFAGAGLFAMYLSFRIASPV
                     LALEVPWHAKLFMTISEPFRWLGDSHVAMLSCVIPLIWAGMGPGCLIYLAALKSVPDE
                     LYEAADMDGATFIDKIIFIVFPILKPLLIINLVGAFIGSWLHATANILAMTGGAARTE
                     VAGLHIFYKAFIYLKFGPATAMAWILALILIGFTVYQLRILSRLEFRTTGDKD"
     gene            complement(7838..8500)
                     /locus_tag="PKK36_05310"
     CDS             complement(7838..8500)
                     /locus_tag="PKK36_05310"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNR94011.1"
                     /translation="MRIIEAGEQVFFLKRTGRETLWRHLVMLFRGHRPKSGALREVQM
                     LHLLKSSGFETMEPVAWGEQRLGLLTVRGFVLVRKVPGKELAGLFDESEGHMQFRLVR
                     EMGHLVGRLHARGFFHPVRLKDLIATNDGLVLIDRETSKPWKSRFFEWRCRSSLIRAL
                     RRMLRDGHPIGPGSVCAFLLGYRDGMSEFRQVRPRELASKICADLRKTKRKPFNQAVS
                     YR"
     gene            8832..10130
                     /gene="tig"
                     /locus_tag="PKK36_05315"
     CDS             8832..10130
                     /gene="tig"
                     /locus_tag="PKK36_05315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNR94012.1"
                     /translation="MQVEVKEAGPCRRELRISFPADRVESEYGKMLAAYKNQAKIPGF
                     RPGKAPVNLVEKRFAKDILEELKERLIGVGYHEAIQQEKMDVVSVLEVRDVSFGRNQD
                     MAFTVMLDIPPVFDLPEYRKIKLQAKEVSVTDDEVQQALTELRQRFGTLEEKSEGTIA
                     RGDLVQMDYEASFGGKKIEEVAPGALRLGRAEGAWLYADEHAFIPGLGEAVIGSAVGD
                     AVSVPVEFGPDFPEKAIAGQKAEYRVTIKGMRQMKMAEINEEFLKRLGAASEEALRED
                     IRTDLQQHKEQEEISRRKNEIMKTLMDAVSFDLPESVVNEETSSAISDIVRSSAQRGV
                     TDEALMEKKDEIFEAASRSAKDKVKLRYILHKIATAENMEVSDADYRNHVARAAMGYG
                     MDPEKLLKDIEGKNRKERIIDDIRVGRALDFVLAEADVQG"
     gene            10144..10770
                     /gene="clpP"
                     /locus_tag="PKK36_05320"
     CDS             10144..10770
                     /gene="clpP"
                     /locus_tag="PKK36_05320"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNR94013.1"
                     /translation="MKSANQTLVPMVVEQTSRGERAFDIYSRLLKERIIFIGTPIDDD
                     VSNLIVAQMLFLQSEDSEKDVHLYINSPGGSVTAGLAIYDTMQFLKCDVTTYCVGQAA
                     SMGAVLLAAGTKGKRFALPNARIMIHQPWGGVQGAASDISIQAKEILRLREMLNSLLV
                     KHTGKTLKTIETDTDRDFFMSADDARKYGLVDEVIVSRKGDAGKQPLS"
     gene            10767..12014
                     /gene="clpX"
                     /locus_tag="PKK36_05325"
     CDS             10767..12014
                     /gene="clpX"
                     /locus_tag="PKK36_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNR94014.1"
                     /translation="MTGKGPVNRCSFCGRAQREVGQLIAGPGVFICDSCAAQAAEMSR
                     PPHAGKKPAPPLEDLKVPAPHEIKAALDEYIVGQQQAKKVLSVAVHNHYKRVNYQHKL
                     LHAESGAGVEIEKSNILLIGPTGSGKTLMAKTLARILDVPFSISDATSLTEAGYVGED
                     VENILLRLLQAADYDLARAQRGIVYVDEIDKIGRRMENVSITRDVSGEGVQQALLKIL
                     EGTVANIPPKGGRKHPEQEYISMDTTNILFICGGAFTGLDEIIQRRAGKGALGFRGEA
                     EKKARPKVEPEDLIRYGLIPEFIGRMPVVSTLEALTEADLVRILTEPRNAMTKQYQQL
                     LAMEGIDLTFTSSALNELARMAKQKGTGARALRSILEHVMLDVMYEAPVKKSAGRKCR
                     ISRASIAQHFETPELKAGRRRSA"
     gene            12054..13511
                     /locus_tag="PKK36_05330"
     CDS             12054..13511
                     /locus_tag="PKK36_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNR94015.1"
                     /translation="MRKRLQVAWICVLLPLAVHARQIPLTILFTSDVNGRLVSADSSG
                     GLLQCAALTRAIRAQRRNVLLLDCGDAVHGSFVSAITGGATVRDAMEEMEYDACVPGR
                     SDFDSGIRGYKGLFETSSVPVLAANIHPPGFMRPFLVKEIDGIRVVVVGLACPVLAGC
                     HLPDKLDGLQVKDSVSALADIMPLVRQMDPDVVILATHQAWQDYDGPGSEINAIVRRF
                     PEIDVILGGRRYRPEYRRLSDSTVYAQAGSGAHHVGMVDIVYDNVSRTITSSSGLAHP
                     VTPSIRPDQVLQKKLARVLKKAQEESRKTAGCLSTPLAGSSLVAAAVMSACGADAAML
                     EDVSRRTLSAGKLTMEEVWDMVPHEREIAVISVTASQMDEILEQEAALGRESSRLIVN
                     GLSWEQYDDREAGARIEQLRMADGAFIHPRKLLRVAAADVTLASSCGRRPHLRALSEE
                     PSSRIEETGLRMRDAVLAYLQANYPCSGGDGADKP"
     gene            13522..14691
                     /locus_tag="PKK36_05335"
     CDS             13522..14691
                     /locus_tag="PKK36_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR94016.1"
                     /translation="MKFKKLSGEGWLQALILLHIFFIPVAISPAQVLLFTAVAAGMFF
                     LLREHRLAEIKSSSYFVPVLLFGLMIILSYFWSVRPSVTAHKFHRIIYYLGLFMTGLL
                     LCPDRRGIRLAVWMFVSGSSVQAVWDLARAPFLVCVRGVNLYDTGNMRDPQLYLVSLI
                     LLAAFFMFGEVRGKNRICWAALVLNAAGLIIHFKRGVWISFFIALLLLVLLTRRWKAL
                     AAAALVAGSLVFVPQVQQRLAGLKDEFRTGQGGRMALWTEVAPALIREHPLGMGYGAT
                     RNEDFTPHTAYVQPKLTHVHNNALQIILELGWQGLAVWILWMGTALVTCGRLVRNPAV
                     KERWVALGGLAALAGLLVNGMVEYNFGDSEILMAYCFLMGLSVLLRRSDAAPAAI"
     gene            complement(14647..15879)
                     /locus_tag="PKK36_05340"
     CDS             complement(14647..15879)
                     /locus_tag="PKK36_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNR94017.1"
                     /translation="MRESAAHIARTLQAHGYTAYFAGGCVRDLLLGREPKDYDIATDA
                     APETVQSLFTHTEAVGRAFGVILVKQETFCFEVATFRTDISYSDGRRPDAVQFCGPEE
                     DARRRDFGVNALFYDPSTEQVIDYVNGREDLKNKTIRAVGNPSERFAEDHLRMLRAAR
                     FASVLDFTVEEQTANAIRNHAGLLDRISVERVQAEIARIFEEAPKPGAALQLLRNLEL
                     LKHIFPELPSLNTEVVLNETADRLNRMRIRTASMGYAVVFFSAADADTAEKAMKRLRC
                     STETVNIVRDAIRGQPDMQNFAAKPESEKRLVLSARGFPIQMELFRLSHPQDAQAFQA
                     LNLYAAEYTDRPLPAHWVRGGDLLKHGVSRGPAMKILLDKAYRAQLDGCFADKEELLN
                     WLLTSDGGRCGVGTTEKD"
     gene            complement(15896..16753)
                     /locus_tag="PKK36_05345"
     CDS             complement(15896..16753)
                     /locus_tag="PKK36_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94018.1"
                     /translation="MDTNRWAPPKRPLLLELLALVFVVLAIAIPTYLQNESTPSLPAQ
                     DTIAAQMQSTREVGGIDRDEWRETSGSLFWGIGILGLYIVHLYIANSSNDRISTSVAH
                     FLSPLVFAMITYYRLYSLKESSIEVVSGSPLEILKWAIGVFLITLLLARLRMVGYRKQ
                     FSDITWDMSTPALYDKTFFELMVYIYPLLYPPRRYHLCPQGILIEGWFYIMPIPFDVI
                     HSVDAMKSASFLSTGTFLAASTKTLTRVQMNDQRVPVYISPEDKTGLLKYCANRLAPL
                     TPDARTPIV"
     gene            16925..>17405
                     /locus_tag="PKK36_05350"
     CDS             16925..>17405
                     /locus_tag="PKK36_05350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR94019.1"
                     /translation="MKNLFMWALIVMSVVFLSGCRPPVAPETPAPEAPDAAAPAPAPA
                     ASRDFDIPSLSVDGGTIALASHKGKVVLLDFWATWCPPCRYELPALMKLYADLKDRDF
                     VLIGMTVDQGSREKVQADVAKFSLTYPVGLAGPDVQAAYGGIRAVPTKFLLGKDGSII
                     "
BASE COUNT         3763 a         4779 c         5175 g         3688 t
ORIGIN      
        1 ggagtccgca caaaaatccc tcagaccgga attgtaacga tcggcgatga tgttgaaatc
       61 ggcgccaaca ccgccattga tcgcgcgcgt ttcggcgtga cccgtatcgg cagcggcgtg
      121 aagatagaca acctggtgca ggtcgcccac aacgttgaaa ttggtgatca tgcggtggtg
      181 gtggcccagg tgggtatttc cggcagttca tcagtcgggc gtcatgcggt gctggccgga
      241 caggtgggtg tggttggtca cgttcacatt ggcgatgggg ccattgtggg agggaagtcg
      301 gttgttacca aagacatacc cccgcgcacc tttgtcagcg gaaatcccgc cggtcctcat
      361 aaggaagaac aaagacttca ggccctcacg cgcaaacttc cggagatgaa gcagaagatt
      421 gccgacatcg aaaagcgcct gcaggaactc gaaaagaagc ggtcctgatc agaatgagca
      481 ggaaacggtg atcccgtatg tgactggatc acctgcctgg gccagagcgg ttgatcctgg
      541 aaatgcatgt acaacgccgg catattcctc atccagcagg ttttttgcaa agagccgcag
      601 ggaccagttc ccgttttcga atccggcctg cacgtcaacc agtgaatacc cgtctgtctt
      661 gacttcattg gcctcgtccc agtaggtgct ggactgcccg tgcacacccg cctgcgaaac
      721 aaactgccac ggcccgccga cagggaaatg gcatcgggca gccacagcat aattgaatgc
      781 cggggcaaag ggcagtttgt tcccgtcaac ttcgggcgat tccgtgtatt ccatgtcggc
      841 atatcccgcc gaggcaaaga tgtccagcca atctgttgcg cgcgctgtga actcggcctc
      901 caggccgcgg ctttcggctt cgccggcatt gcggaaataa taactgacgc tgttgggcgc
      961 aaactgcatg agctgctggt cctcaatgcg aatatagaat acggaaagat tgagcatgaa
     1021 ccggtctgcc ggcccgccgg ccttgatgcc cgcctcgtag ttccatgaaa tttccggatc
     1081 atagtcggga tcggcgccgg gaatggggct gttgaatccg cccgcgcgcc agcccctggc
     1141 gattctggca taactcagca gattgccggc ccacatgtaa tccaatgcga tttccggcga
     1201 ccattccttc tcggtccgcg catcgtccga agccatgctc atgccgggga tgggaacccc
     1261 gcccatccgc cttgtaatct gtttattctg ctccagccgg tcgtggtcgt agcgcaatcc
     1321 cgcgcgtaca gacgcttttt cgccgacggg ctgaataatg tgtccaaaag ccgcgagtgt
     1381 ataggcgtcc aggtcctgca tggcatgttc agcaaatccc ggaacggcgc catacatggc
     1441 cccgtcactc ccgaaagagc tggtgttctc atcctccccg cggcttgtcg agaaatagcc
     1501 tccggccatc cagcgcagtt catcgcggcg gcttcctgac gaaagccgca gctcctgtgt
     1561 gaaagcttcc agccagtcct tgtcgttcat ggacatgatg tcagccgggc tgaagtctga
     1621 atcataaaca tcgtggtttt cccagtccgt gaaactcgtg atggactgga gcgcgagcct
     1681 ttcgcccgtc actttcacgt tcagcgcaat caggctcaag tcgcgctcgt gggaaccggg
     1741 aatgttcatg ttcacatgat gcggattgcg ttcaacgtct gcgagcgggg ccagcgcgta
     1801 gccgccgtcg gagtcgcggt cgcccagcag cgtgagacgc atatccagcg tgtcgttcgg
     1861 tacggcgcgc agcgagagca ttccgccttt ctgtgtgatg ttgtcggccc tggtgtccag
     1921 aaaatcattt tccacatagc cgtcccgttc gcggtaatac atgcttgagc gaaatgaaaa
     1981 cttcccgtca tccgtcgcgg cgcctgacga tgcgcgggtc tggaaatagc tgtcgctgcc
     2041 ggcggtctgc tcgatgcgcc cgttcaaaac atctcccggc gccaccgttt ggattttcac
     2101 cgcaccggcc tgtgtgttct tcccgtaaag tgtgctttgc gggccgcgca ggatttcaat
     2161 ctgctccaca tcatgcagtt caaggtccga aaacccggtc ttgtgcagat agacatcgtc
     2221 cagaaaaaca ccgacggcgg gcggggtggg gccggaggag ccgatgcccc gcaggtagta
     2281 aagcgcggtg cgccggcttc ccgatgaata gaagctgaat cccggagtca gactggaaat
     2341 gtcctgcatc gaatgcagat gattccggga aagactgtct tgcgtgaaaa cggacaggga
     2401 tagggggagt gtctgcggat tctcgtcgaa tttcgatgcg gttacggtga tggacccaag
     2461 attgagcggc gtatttccgg gggaatcttc tgcatttgca cagagaggaa gcagtgccgc
     2521 agtcagagtc aataagcatc ttttcatctg gttgagcctt tcatttgagc cgataagata
     2581 gttgctgtgc cggatgctgt aaagctccga cgttcattca ttgatttccg gccgccacgg
     2641 gatatattga atgaaaatcc atcgaggtgt tatgtctttt gtccgcatga ggggctgcaa
     2701 aatgctggtg tttgtgccgg aggagcagag cgcctccggg aaaaagcaca actgccgcga
     2761 ctgtttttcc tgccagtggt gcagcgacga gcgctgcaaa gtgtgcctga accgcaaaaa
     2821 cacttcctgc cggcgtcact cctccaaatc ctgcaggaaa gcgcgcaaac atccgagaac
     2881 cgaaaaatac tgattttgag gccccaaaag cccaaaaact gcccaaaatg gctcaaaaac
     2941 agccaaaaac agcccaaaac ggccccaaaa ggcttaaaaa ggctcaaaaa acgttaaaat
     3001 acgggcaaaa accgtaaacg gacgatatag tactaaatta gtactatatt ccttcatgac
     3061 cgttttttaa ttggagggcc gacctctgtg tcggccgtat gttcactttt ccaccggcac
     3121 gacgatgccg cggatgatga ttttctggca gacggcgaag atgatgaagg tcgggacggc
     3181 cgctatgatg agcgaggcgt agatgacggg ctggccgctc cgctgctgca gctgatagag
     3241 ccacaccatc agcgtccaca ttttctcgtc ctggcagatc agcagcgcga acatgaaatt
     3301 ggaataggcc atggtgaacg cctgcagcgc gatgaccgac aggatcggct tcgaaaggct
     3361 catggtgatg tgccagaaca tcgtccattc attggcgccg tccagttcgg cgcattcata
     3421 gagttcgcgc ggaagcgaat cgaaaaatcc cttcagcaga aagatggcat agccgttggc
     3481 gaggcccggg agcaccagcg ccgcgaatgt gttcagcagc tgcattttcc gcagcatcaa
     3541 aaacaccgga atctgcgtga ccatcggcgg gaaggccatc gtcgccatca ggaagagcag
     3601 gattttgtag gtggagggca tgcggttgcg gctcatggca taggcggcca ggggattgac
     3661 aatcagggca cagagaaccg ccagcgcgca gtagatagcc gtgttcagga tgcctcgccc
     3721 gtgcagcacg atgtattcaa ggaccgtctt gaagttgcgc gtggcgaatt cccatgccat
     3781 gtcttttttc atgttcagga acgcggcgca gtgcagctcc ctttggggaa tgggtgtttt
     3841 atcgaagaac ggtttcgacg gaacgaaacc ctccatcgtt tccgctttca gccagtcacg
     3901 gaagcggaat tccagtgtgt ccacgcgcag gaattccgcg ggaataatgt ggagtcgtcc
     3961 cgtgtcgggg tcctcccatc cagtgatgaa cgattcccag tcgctgagga caatgccctc
     4021 gaacggaggt tcttcaacga gcgggatgtc attgaacgaa cggtgtgcgg tgccgcagat
     4081 gcggttcagg gttttgattt tcccgtattt tgcctcaaga aaatcgtgat aaagttctac
     4141 cgccgccgga tcagcccgca cccacaggag attcagcgtg tgacgtacga agttctccca
     4201 gtctgccttt tccgccgctg ttccatccgg cagcgtgcgg ctgagtgtga tttcatcata
     4261 cgagtcaaag gcggtgccat gtgccttgtt ataggcggca atgtctccgg aatactgcgt
     4321 tttcaggaaa agattcctgt aaaatccgtc agcggagaaa taataaaaca gcgcgggatc
     4381 cagtgatttt ttaaactcct gcaactcagc gctgaagggg tcggtgcgcg gcatgtcgcg
     4441 gcggagcatg aagttctccg gaatcaggaa gaaggcgttc catccgatga agtcggtgcc
     4501 cagcgcgcgg tttacgtccc cggccgattt cccgtatttg cctgccaggt gctttttgaa
     4561 gttccgcagg ttcatcggaa tggtcttgga aattcgagcc tggacaaagc cgcaggccga
     4621 cgcataggcc ggcggcggat tttcagacag gaaccctgtc cacagttcaa tttgtccgaa
     4681 atcaatgttt tcaggcggct caacttcctc aaaactccgg taagaagtgc cgtacaccat
     4741 gttcattgtg tccagcgact cgttgaagag cccctcgata tattttcggt agagcatggt
     4801 gtcgtcgcgg aggaagcggg ggatgatgtc gaggtctccc agatccaccg cactcttggt
     4861 gctgccggac agcatcacga ggaacggata cagcatggtc gcggcgccca gcgcgagcag
     4921 ggcatagatc agggcgtaca gcgtcctggt cttccatgcc tttctgccga tctgtgagat
     4981 aatgggcacg gttagtcctt gtcccctgtg gttcggaatt caagccgtga aagaatgcgg
     5041 agctgataga ccgtgaatcc gatcagaatc agcgccagaa tccatgccat ggccgtggcc
     5101 gggccgaatt tcaggtaaat gaacgccttg tagaaaatgt ggaggcccgc cacttcggtg
     5161 cgcgcggcgc cgccggtcat cgcaagaatg ttcgcggtgg catgcagcca tgatccgatg
     5221 aatgcgccga ccagattgat gatcagcagc ggcttcagaa tggggaagac gatgaagatg
     5281 attttatcaa tgaaggttgc cccgtccatg tcggcggcct cgtaaagttc gtcggggaca
     5341 ctcttcagcg cggccagata aatcaggcat cccggcccca tgccggccca gatcagcggg
     5401 atcacgcacg acagcatggc cacatgcgaa tcgcccagcc agcggaaggg ctcggagatg
     5461 gtcatgaaga gtttggcgtg ccacggaact tcgagcgcaa gcacaggcga agcgatccgg
     5521 aaggaaagat acatggcaaa aagacccgct cccgcgaaca gcagcgccca cgtccgccgc
     5581 ccgtgataaa gaagccgccg cgtgaatgaa cacgccagaa tgaaaaggaa caggccaatg
     5641 gccagatatc cggccgccgg aatgcgcatc atcagggcgt tcagaaggcc gcgttcggtc
     5701 ggatcataaa aagttttcca gagcaggatc accacgagtc ccgacatcac ggccggcaga
     5761 tagtagattg tgcgaaacag cagtttgccg cgcggaatct cctggaggag aatcgcgaga
     5821 atcactggcg gcaggaaggt gaggccgatc accagcagac agtagcgcac agagttccac
     5881 accgcggccc accattgagt gtcccagagg acacttccga aattgtccag cccaacccag
     5941 ctcgaccgcc ccatgatctg gtagtcctga aacgccatca ccgaaccgcg aaccagcggc
     6001 agatactgcc agaggaaaat ggtgaggagg gcgggcatca gcagcagcca tgcccagacc
     6061 gtgcgtctcg ggttccagct ctcgccgtgc tgccgttcgg cctttggcgg cgtgaaggcc
     6121 cggccgatcc gtatgaaacc ccaaacgaat gcagtgcata tcccggccag cgcgagcagg
     6181 gccgtgagcc ggtggatgcg cagcgtggcg ggcggcaggg tccccagcat ttcctcgcgg
     6241 gctttctgcg ccgactgcct cagtagtttc tgaaaaagcg ccagtcgctc gtcgcggccg
     6301 gcgggaagcc ggccctgcag cgcaagctcc tcggccgttt cgatcggccg ggtcatcagg
     6361 tcatagatcg cgttgctgtt cttcccgtag ggttcgggcc ttccggtttc aagcgcgatg
     6421 tcaaagcact ccttccagcc cctgggggcg aggcgcacga gttcggggta accgaacatt
     6481 tcgagatagc gcggattgat aaaccggccc aggccgcctt cgaccatgat ccgcgttttg
     6541 atccgcgcgg catccttgct gtcgtagaac cggatatatt cccatgccgc atcgcgcacg
     6601 gccgcatccc tgatgcctgc aaagagcccc atcatcatgg agttcagttc cgcgccgcgc
     6661 tcgccgcccg gcccgatggg taccggggcc atgccggtga tgtcgggatt gatacgctga
     6721 aaaaccttct cgtcaatgta ggcgatcatc atggcgattt cgccgcggtc ccacttggcg
     6781 gcggtggaac cccagtcggc gtcgcggtgc gcatagccgt accgcctgcg gttttccgca
     6841 tcggtccatt cctccgtgca gagccggttg tagaaatcca gcgcgacggc ggcttcgggc
     6901 gaatcaaaat caatggtcca tgcaccggat gattcatccc acgtcatgat ttctccgccg
     6961 gccgaccaca ggaaagtgat ccaccaccac gactcgtgct tgcccttgct gaaatgaatg
     7021 ccgtagatgc cgcgccccgg atcggcgatg tttgtgcagg cgctgtagaa atcgtcccag
     7081 gtccagttat ggtgaggata ggggacgccc gccgcgtcga acagatcctt gcggtagatc
     7141 accaccttgc cgagcgcgcc gccgtagggc atggcccaga cctgcgtgtt tctgccgggg
     7201 ccctgccgcc ggatcacggg ccagattttc ggatgcacgc ggaaatccag ttcttcctgc
     7261 gtcatggcgg agagatagcc gtcctcgggc ttgtccattg gatagaggaa tccctgctga
     7321 atgtaggtgt ccgacattcg gaagttcaca tacatcacat ccggcgcgac accgccggcc
     7381 acggccagca gttcgctccc gacaccctcg acctggatgc cggaaaaacg gtgcagttcg
     7441 atttcgacat ggtcccagtt gtgccgtccg tatttctccg gattcgcctg atatttttcg
     7501 cggcagcgtt ccgcaaagat ttctgggaaa cgacgcacaa actccttcac tgcggcggcg
     7561 tctgcgcgcg tggatgtgtc ggtgcggctg gggtcgggga ggtcgaacag cttcacatgg
     7621 atgacggtct tgccgtcgcg gtcttcaatc catccgccct gcacggcgca ggccgcacag
     7681 agaacgagcg ccagactgaa aactctccat ttgcagttca tgccggcatc ataagcattt
     7741 tccggcgttc atcaggagaa aaaaaggaat gcagggccgg gtttgatgat tggttttaaa
     7801 aaagccgggc ggtgcccgaa atattcatgc cggaaagtca tcggtatgaa accgcttgat
     7861 taaagggctt tcgttttgtc tttctgaggt cggcgcagat tttcgaggcc agttcacggg
     7921 gcctaacctg cctgaattcg gacatgccgt cccgatatcc cagaaggaag gcgcagacac
     7981 tccccggtcc gatcgggtga ccgtcgcgaa gcatccggcg cagggcgcgg ataagcgaac
     8041 tgcggcaccg ccactcaaag aaccgtgatt tccagggttt ggaggtttca cggtcgataa
     8101 gaaccagccc gtcatttgtt gctatcaaat ctttaaggcg aaccgggtgg aagaaacccc
     8161 gtgcatgaag acgtcccacc aggtgtccca tttccctgac gagcctaaac tgcatgtggc
     8221 cctcggattc atcaaagaga ccggccagtt cttttccagg caccttccgc acaagcacaa
     8281 atccacggac ggtgagaagt cccagtctct gttctcccca tgccacgggc tccatggttt
     8341 caaatccgga ggactttaga aggtgcagca tttgcacttc gcgcaaagct ccggattttg
     8401 gccggtgccc gcgaaagagc atgaccaggt gcctccacag ggtttcgcgc ccggttcgtt
     8461 tcaggaaaaa gacctgttct ccggcttcaa ttattcgcaa ttccctccca gcggggcctc
     8521 tggcgaggag tattcctgca gaatcgctgt tcagccagtg atccatctgc ctgccgagcg
     8581 ggaacagggg ttgcagctct ggtctaatgt gaaaattcgg catggtcaaa attggtggtc
     8641 tgcgggcata taattcataa tcgttgaacg tgaagctaac aggcgcggcg gggtgccgcc
     8701 agccaaaaat ggcccggccg catatgactg cgggtctctg ctgcccgtcg gggcatttcc
     8761 gtcgttgact gtctctactg ctatgaatta tcgtattcac cagcaattta cagatgaagg
     8821 agacacagca gatgcaagtt gaagtgaaag aagccggccc ctgccgcagg gaattgcgta
     8881 tttccttccc ggcggaccgg gttgagagcg agtacggcaa aatgctcgcc gcatacaaga
     8941 accaggccaa gattcccgga ttccggcccg gcaaggctcc tgtcaatctg gtcgaaaagc
     9001 gttttgcaaa ggatattctg gaagagctga aagagcggct cattggtgtc ggttatcacg
     9061 aagccattca gcaggagaag atggatgtcg tttccgttct ggaagtgcgt gatgtgagct
     9121 tcgggcgtaa tcaggacatg gctttcacag tgatgctgga tattcccccc gtgttcgatc
     9181 tcccggaata ccggaagatc aagctccagg caaaggaagt gagcgtgacg gacgatgagg
     9241 tgcagcaggc gctgacggaa ctgcgccagc ggtttggcac tcttgaggag aaatcagagg
     9301 gaaccattgc ccggggagat ctggtccaga tggattatga ggcatccttc ggcggaaaaa
     9361 agatcgagga agtcgctccg ggtgcgctgc gcctcggccg cgcggaaggc gcctggctgt
     9421 atgccgatga acatgccttt attccgggcc ttggcgaggc ggtgatcggg tcagcggttg
     9481 gtgatgcggt cagtgtcccg gtggaatttg gccctgattt tccggaaaaa gcgatcgccg
     9541 gacagaaggc agaataccgc gtcaccataa aggggatgcg ccagatgaaa atggcggaga
     9601 tcaatgaaga gtttctcaag cgcctcggag cagcttcgga agaagccctg cgggaagaca
     9661 tacgaaccga tctgcagcag cacaaggagc aggaggaaat cagccgcagg aagaacgaaa
     9721 tcatgaagac cctgatggat gccgtttcct ttgacctccc ggaatcggtc gtcaatgagg
     9781 aaaccagcag cgcgatcagc gatatagtcc ggagttctgc gcaaaggggc gtcacggatg
     9841 aagccctgat ggagaagaag gatgaaatct ttgaggccgc ctcgcgctcg gccaaggaca
     9901 aagtgaagct gcggtatatc ctgcataaaa ttgcgacggc ggaaaacatg gaagtatccg
     9961 atgcggacta tcggaatcat gtggcgcgcg ccgcgatggg ctacggcatg gaccccgaga
    10021 agctcctgaa ggacatcgag gggaagaaca gaaaagaacg aatcatagac gatattcgcg
    10081 tgggccgcgc gctggatttt gtgctggccg aggcggatgt ccagggataa cggagaagca
    10141 gcgatgaaat cagcaaacca gacactcgtc ccgatggtgg ttgagcagac cagccggggg
    10201 gagcgcgcct ttgacattta ttcgcgactg ctgaaagaac gcattatctt tatcggcacg
    10261 ccgatcgacg atgatgtcag caacctgatt gtcgcacaga tgctttttct gcaaagcgag
    10321 gattctgaaa aggacgttca tctctacatc aacagccccg gtggatcggt aacggcgggg
    10381 ctggccatct atgacaccat gcagttcctg aaatgcgatg tgacaacgta ttgtgtggga
    10441 caggccgcca gtatgggggc ggtgctgctg gctgcaggaa ccaaaggcaa gcgctttgcg
    10501 cttccaaatg cccgcatcat gatccatcag ccctggggcg gggtgcaggg cgccgcctcc
    10561 gatatcagca tccaggccaa agagattctg cgattgcgtg aaatgctgaa cagtctgctg
    10621 gtcaagcaca cgggcaaaac gctcaagacc atcgaaaccg acaccgatcg cgattttttc
    10681 atgtcggctg atgacgcgcg caaatacggg ctcgtggatg aagtgattgt cagccgcaag
    10741 ggagatgcag ggaagcagcc gttgtcatga caggaaaagg tcccgtgaat cgatgttctt
    10801 tctgcggccg cgctcagcgt gaggtcggac agctgattgc cggcccggga gtattcatct
    10861 gcgacagctg cgctgcccag gcggcggaaa tgagccgtcc tccgcatgcc gggaagaagc
    10921 cggccccgcc gcttgaggat ctcaaggtgc ctgcgcccca tgagatcaag gccgccctgg
    10981 atgaatatat cgtcgggcag cagcaggcca agaaagtgct gtctgtcgct gtgcacaatc
    11041 actacaagcg cgtcaactac cagcacaaac tgcttcatgc agagtccggt gcgggggtgg
    11101 agatcgaaaa gagcaatatc ctgctgattg gtccgaccgg aagcggaaag accctgatgg
    11161 ccaaaacgct ggcccggatt ctggatgtgc cgttcagcat ttccgatgcc acttccctga
    11221 cagaggcggg ttatgtggga gaagatgttg aaaatattct tctgcgtttg ctgcaggccg
    11281 cggattatga tctggcccgc gcccagcggg gtatcgtata tgtggacgag attgacaaga
    11341 tcggccgccg catggagaac gtttcaataa cgcgcgacgt gtccggcgag ggtgtgcagc
    11401 aggcgctgct gaaaattctg gagggcacgg tcgccaatat cccgcccaag ggaggccgca
    11461 agcatcctga acaggaatat atttcgatgg acaccaccaa catcctgttc atctgcggcg
    11521 gcgcgttcac gggacttgat gaaatcatcc agcgccgggc cggcaagggt gcgctggggt
    11581 tccgcggcga ggccgaaaag aaagcaaggc cgaaggttga gccggaagac ctgatccggt
    11641 acggactcat tcctgaattc atcgggcgca tgccggtcgt ttccacgctt gaagcgctga
    11701 ctgaagcaga ccttgtgcgt attctcaccg aacccagaaa cgccatgaca aagcagtatc
    11761 agcagctgct ggccatggag ggaattgacc tgacctttac atcatccgcg ctgaatgaac
    11821 tggcccgaat ggcgaaacag aagggtaccg gcgcgcgcgc gcttcgctcc atccttgagc
    11881 atgtgatgct ggatgtgatg tacgaagcgc ctgtgaaaaa gagcgccgga aggaaatgcc
    11941 ggatatcccg ggcgagcatc gcccagcact tcgagacgcc ggaactgaag gccggtcgcc
    12001 gccgaagcgc ctgatgactc tgtgtccggc tcatgtgcgg ggaagacgga agaatgagaa
    12061 aacgtctgca agtcgcatgg atatgtgttc tgctgccgct ggctgttcac gcccggcaaa
    12121 tcccgctgac gattcttttt acctcggatg ttaacggccg gctggtctcg gcagattctt
    12181 caggagggct cctgcagtgt gccgccctga cccgcgctat ccgcgcacag cggaggaatg
    12241 tcctcctgct ggactgcgga gacgccgtgc atgggagttt cgtgagtgcg atcaccggcg
    12301 gcgccaccgt tcgcgatgcg atggaggaaa tggaatacga cgcctgtgtt cccggacgaa
    12361 gtgattttga ttctggaatt cgggggtata agggcctttt tgaaacatcc tctgttcccg
    12421 tcctggcggc caacatccac cctccgggct tcatgcggcc ttttcttgtg aaggaaattg
    12481 acggtattcg ggtggttgtt gttggcctgg cttgtcctgt gctggccggg tgtcatcttc
    12541 ctgacaagct tgacggtctg caggtcaaag actccgtgtc cgcgctggcg gatatcatgc
    12601 cgttggtgcg ccagatggac ccggatgtgg tgatcctggc cacgcaccag gcatggcagg
    12661 attacgacgg gccgggaagc gaaatcaacg ccatcgtgcg ccgcttcccc gaaattgatg
    12721 tcatcctcgg cggccgccgc taccggcccg aataccgcag actttccgac agcaccgtct
    12781 atgcccaggc gggcagcggg gcgcatcatg tcggcatggt ggacattgta tatgacaatg
    12841 tgagccgaac catcacgtca tcgtcaggcc tggcgcaccc tgtaacgcca tccattcgcc
    12901 ccgatcaggt tcttcagaaa aaactggcgc gggttttaaa gaaggcgcag gaagagagcc
    12961 ggaaaacggc cggctgtctt tccaccccgc ttgccggatc atctcttgtg gcggccgcag
    13021 tcatgtcagc atgcggcgcg gatgcagcga tgctggagga tgtctcccgc cggacgttgt
    13081 ctgcgggcaa actgacaatg gaagaggtat gggatatggt tccccacgag cgcgaaatcg
    13141 ccgtgatttc cgtcaccgct tcgcagatgg atgaaatcct cgagcaggaa gccgcccttg
    13201 gcagagagtc ctcgcgtctg attgtgaacg gtctctcctg ggagcagtac gatgaccgtg
    13261 aggctggagc ccgcattgag cagcttcgca tggcggatgg cgcttttatt catccccgga
    13321 aactgctgcg cgtcgctgct gcggatgtca ccctggcctc atcctgcggc cgcagaccgc
    13381 atttgcgcgc gctttcagaa gaaccgtcct cgcgcattga ggaaaccggg ctccgcatgc
    13441 gcgatgcggt tctggcctat cttcaggcga attatccttg ctccgggggg gatggggcgg
    13501 ataaaccgta agcatatgaa tatgaagttc aaaaaactat ccggagaggg gtggctgcaa
    13561 gccctcattt tgcttcatat cttcttcatt cctgttgcca tttcccccgc ccaggtgctg
    13621 cttttcacag cggtggcggc cggaatgttt tttctgctgc gggaacatcg gcttgcggaa
    13681 atcaaatcaa gttcctattt cgtgccggtg ctgttgttcg gtctgatgat cattctgtct
    13741 tatttctgga gtgtcaggcc ctctgttacg gctcacaagt ttcacaggat catctattat
    13801 ctgggattgt ttatgacggg tctgctgctg tgcccggacc gccggggcat ccgtctggcc
    13861 gtatggatgt ttgtttccgg atcaagtgtg caggcggtgt gggatctggc gcgggccccg
    13921 tttcttgtgt gcgtccgcgg agtcaatctg tacgataccg gaaacatgcg cgatccgcag
    13981 ctgtatctgg tgtcgctgat tctgcttgcc gcgtttttca tgttcggcga agtgcgaggg
    14041 aaaaaccgga tttgctgggc tgcgctggtg ctcaatgcgg caggcctgat cattcatttc
    14101 aagcgcggcg tttggatttc ctttttcatc gcgcttctgc tcctggtgct gctgacgcgc
    14161 agatggaagg cgctggccgc cgccgcgctg gtggccggct ctcttgtgtt tgtcccgcag
    14221 gtgcagcagc ggctcgcagg actgaaggac gagttcagga cgggacaggg cggtcgcatg
    14281 gctctctgga ccgaagtggc tcccgctctg atccgggaac atcctctggg catgggatac
    14341 ggcgcgaccc gcaacgagga tttcactcct catacagcct atgtgcagcc caaactgact
    14401 catgtccaca acaacgccct tcaaataatt ctggaactgg gctggcaggg tctggctgta
    14461 tggattctgt ggatggggac cgctctggtg acctgtggcc gccttgttcg caaccccgca
    14521 gtgaaggagc ggtgggtcgc gctgggcggc ctggccgcac ttgcgggact gcttgtgaac
    14581 ggcatggtgg aatacaactt cggcgacagc gaaatattga tggcctactg cttcctgatg
    14641 gggctgtcag tccttctccg tcgttccgac gccgcaccgg ccgccatctg acgtcagcag
    14701 ccagttgagc agttcctcct tgtcggcaaa gcatccatcc agttgtgcgc ggtaggcttt
    14761 gtccagaagg attttcattg cgggaccccg agacacgccg tgtttcagca gatccccgcc
    14821 gcgcacccag tgagcgggca gtggccgatc agtatattca gcggcataca gattgagcgc
    14881 ctgaaatgcc tgcgcatcct gagggtggct taagcggaac agttccatct ggatggggaa
    14941 tccgcgtgcg gaaagcacca ggcgtttttc gctttccggt ttggctgcga agttctgcat
    15001 gtccggctgc ccccgaatgg cgtcgcgcac gatgttcacg gtttctgtgc tgcagcgcag
    15061 acgcttcatg gctttctctg ccgtgtccgc gtccgcggcc gagaaaaaaa ccacagcata
    15121 acccatggat gcggtgcgga tgcgcatgcg gttcagcctg tcggcagtct cattcaggac
    15181 aacttctgta ttcagcgagg gaagttccgg aaagatatgc ttcagcagtt ccagattcct
    15241 gagcagttgc agggcagcac ccggctttgg ggcctcttca aaaatgcgcg cgatctcggc
    15301 ctgaactctt tccacactga tgcgatcaag gagtccggca tggttcctta tggcattggc
    15361 cgtttgttct tccactgtga aatcgagcac ggaagcaaac cttgcggccc gcagcatgcg
    15421 cagatgatcc tcggcaaagc gttccgaagg atttccgacc gcgcgaattg ttttattctt
    15481 cagatcttca cgcccgttca catagtctat gacctgttct gtggagggat cataaaagag
    15541 cgcattaact ccgaaatccc tgcgccgggc gtcttcttcg gggccgcaga actgaacggc
    15601 atccggacgc ctcccgtcgc tgtaggagat gtctgtccgg aaagtggcta cttcgaagca
    15661 gaaggtttcc tgttttacca gaattactcc aaatgcgcgc ccaacggcct ccgtgtgtgt
    15721 gaagagggac tgaaccgttt ccggagctgc atctgtggct atatcataat cctttggttc
    15781 gcgccccagc agcaaatcgc gcacacagcc gccggcaaag taggcggtat atccatgggc
    15841 ctgcaatgtg cgcgcgatat gagcagcaga ttcacgcatg cggacgctgc gtgattcaga
    15901 caatcggggt gcgcgcatct ggagtgaggg gtgccagccg gtttgcgcag tatttcagca
    15961 ggccggtctt gtcttccggc gagatgtaca cgggaacccg ctgatcattc atctgcaccc
    16021 gggtcagcgt ctttgtggac gcggccagaa aagtgcccgt gctcagaaag ctggcagatt
    16081 tcatggcgtc cacagagtgg attacatcaa aggggatggg cataatgtag aaccagcctt
    16141 cgatcagtat tccctgagga cagagatgat agcggcgcgg gggatagagc aggggataga
    16201 tgtaaaccat cagttcaaag aatgtcttgt cgtagagcgc gggagtgctc atgtcccagg
    16261 tgatatcgga aaactgcttg cgatagccga ccatccgcaa ccgcgccagc agaagtgtga
    16321 tcagaaaaac cccaatggcc catttaagaa tttccagcgg agatccgctg accacttcaa
    16381 tcgaggattc cttcagcgaa tacaggcgat aataggtaat catcgcaaaa acaagaggcg
    16441 agagaaagtg cgccaccgaa gtggatatcc ggtcattgct tgagttggcg atatagaggt
    16501 ggacaatata gaggcccaga attccgatac cccagaaaag actcccggag gtttccctcc
    16561 attcgtcgcg gtcaatgccc ccgacctcgc gggtgctctg catttgggcg gcgatggtgt
    16621 cctgcgccgg aagagaagga gtgctctcgt tctgcagata ggtggggatg gctatggcca
    16681 gcaccacaaa cacaagagca agcagttcaa gaagcagcgg ccgctttggt ggtgcccatc
    16741 tgttggtgtc cataaaattt cctgtagctg tttccttgtc ttcccagcaa ctccatcttt
    16801 atgcaggtat atactacccg ccacctgcac tgaggcaagc aaaaacacgt aaatatgagc
    16861 tttcgcattg aaactgcttc cgcgtggggt aaagttaccc aaatagcgcg agaaaggatg
    16921 ttgtatgaag aacttgttta tgtgggcgtt gattgtgatg tcagtggtgt ttctctccgg
    16981 ctgtcgccct cccgtggcgc ctgaaactcc tgccccggag gctccggacg ccgcggcgcc
    17041 ggccccggcc ccggcagcgt ccagggactt tgacattccc tctctgtctg tcgatggagg
    17101 caccattgcg ctggcatcac acaaagggaa ggttgtgctg ctggacttct gggccacttg
    17161 gtgtcctccc tgccgttatg agcttccggc cctgatgaag ctttatgccg atctcaagga
    17221 ccgcgatttt gttctcatcg ggatgacggt ggatcagggc agccgggaaa aggttcaggc
    17281 ggatgtcgcg aaattcagcc tgacctatcc ggttggactg gccggccctg atgtccaggc
    17341 cgcctacggc ggcattcgcg ccgtgcccac taaattcctg cttgggaaag acggcagcat
    17401 catcg
//
LOCUS       DAOQWR010000046        17371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3670_length_17371_cov_4.877166, whole genome shotgun sequence.
ACCESSION   DAOQWR010000046 DAOQWR010000000
VERSION     DAOQWR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17371
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3670_length_17371_cov_4.877166"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKK36_05355"
     CDS             complement(<1..1376)
                     /locus_tag="PKK36_05355"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNR94020.1"
                     /translation="MKTICLPGVIGLLLLIAAAGCSRPEGREILWADLVNELKDTDRM
                     ARLDVRPTQIVTSYDRRGGNDDFNNFARRGPDGWVVLADLEGPGYMSRFWFTGALDGS
                     HGLRMYFDGERKPRIDTTIGDFCGGTEPFIAPLACFENYCWYSWVPIPFRKKLTVMVQ
                     DGGAKQTEGGKPGDERRLFFQINWSRLNEGETIQSWPRDMTDAHKTALSELGARWKST
                     PGANVPGERHEAICKQKSDTAVCELPGPAVIREFRVSLDDSSIGSAVARRQLLRDMIL
                     RISWDGSSPSVNVPLGDFFGSMGQRLKYDSLFFGYRDGVFSIRFPMPFAQSAKIEIQN
                     LSSWDVPLTMEAVVEKLPSWPAHYGYFHAAWRKSGEEMMGRPHNVLRTGGTGKFAACL
                     LGATSFDRSWWLLESDETMRMDSAPEPQWRGTGLEDYFNGGWYYQNVIARPLNGIVFK
                     APFRAVQYR"
     gene            complement(1437..2630)
                     /locus_tag="PKK36_05360"
     CDS             complement(1437..2630)
                     /locus_tag="PKK36_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR94021.1"
                     /translation="MTNKLIHTAPLILMGFVQVACSDTIHLKNGRVIDGEVISADAEY
                     IEIKLPFAGENFTRIRRKDVTQILRDDGTAPAGFPVNEPVELRPSTPVADAVMEQPVQ
                     EDVDYQSLVSAEKMEAFEQGLSPDGTYDAHASLSDDADYKKRNPALLGLAACGDIRGI
                     QKLEQMLLQTQSSTPPKASAAVILDALEKAGSPSSVAVMARIARSGRAAADRRAAVWS
                     LGKLGFPEAMAVVEQVARDSDAGVRAGAAFAAGRLNTEASRGVLVRLLQDPEMGPKLE
                     AAKAVENNPFPAAVPALIGALSDRYAAAPACLALGKLKSREAVPHLLELLNSTDPEYG
                     QVRQQAARGLGMIGDPQAIDPLVALLGDTSGLGHMCAHALSLMVEDGPASQKPSDWLE
                     WAEKR"
     gene            2751..3698
                     /locus_tag="PKK36_05365"
     CDS             2751..3698
                     /locus_tag="PKK36_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR94022.1"
                     /translation="MSTSKKQILVTGGAGYLGSIMVPELLRLGHSVTVVDNFMYQQAS
                     LLDCCADKKLQVIRGDARDEALMKKAVAGADVIIPLACLVGAPLCSQKPIEARTINLD
                     AIKMLVDMSSKDQMIISPTTNSGYGIGEKGKFCTETTPMRPVSLYGKLKVELEEYLLA
                     SGRAISLRLATVFGISPRPRLDLLVNDFTFRAVNDRFIVLFEAHFKRNYIHVRDVAGA
                     FIHALDNFETMKNEPYNVGLSDANLSKMELCLEIRKQIPDFCIMESEVGEDPDKRDYI
                     VSNDKIEATGFKPKHSLQDGITELIKGFQIIRRNQYSNI"
     gene            3709..4002
                     /locus_tag="PKK36_05370"
     CDS             3709..4002
                     /locus_tag="PKK36_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNR94023.1"
                     /translation="MNCFTASKDGVVISVKIIPNAPRCKVDGLLGDYLKIRIQAPPVD
                     GKANAALLKFLAKELGVPVSHLTVLSGHTGRIKRIAVSGVNKQQAECSLAPGT"
     gene            complement(4006..4848)
                     /gene="panC"
                     /locus_tag="PKK36_05375"
     CDS             complement(4006..4848)
                     /gene="panC"
                     /locus_tag="PKK36_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNR94024.1"
                     /translation="MRIIESILEMQHFASELRQQGRRIGFVPTMGYLHEAHLSLMRTA
                     KERADVVAVSIFVNPKQFNVAADLAAYPRDFERDRKMCEAEGVDIIFHPPVEEMYPPG
                     ETTYVNNDFLSRELCGATREGHFRGVLTVVAKLFNIVMPDVAVFGQKDAQQLRILQQM
                     VKDMNFPLEIVPGPTVREPDGLAMSSRNVFLTGEERLQALCLRQALDIAEKMYAGGER
                     SASAVKKAMRAHIEKYPAARIDYVEVVKWETLQKTETLDGKSLVALAVWVGKPRLIDN
                     TILG"
     gene            complement(4848..5645)
                     /gene="panB"
                     /locus_tag="PKK36_05380"
     CDS             complement(4848..5645)
                     /gene="panB"
                     /locus_tag="PKK36_05380"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNR94025.1"
                     /translation="MNKPWTVLTIRAQKGQKKIACVTAYDYSSARLADEAGIPLILVG
                     DSLGMTVLGFESTLPVTMDEMLHHTRAVSRAVRQAMVVADMPFLSYQGSEDEALINAG
                     RFLKEAGADAVKVEGGAVRVELVRRMAANGIPVQAHLGLTPQSVNAFGGFKVQGKTEQ
                     AAERLRKDAKDLADAGAFSLVLECIPQKLAADITASISIPTIGIGAGPGCDGQVLVWH
                     DLLGLYHGQQPRFVKRYAELGTEISRALTGYREEVEQGRFPTEEHGY"
     gene            complement(5648..6151)
                     /gene="folK"
                     /locus_tag="PKK36_05385"
     CDS             complement(5648..6151)
                     /gene="folK"
                     /locus_tag="PKK36_05385"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNR94026.1"
                     /translation="MEAGFSMGSNIGDRQDFLHRALLALTEGGDVEILAKSRLYETRP
                     VDVRDCYKNMAFLNVVVILESVRTPLEWLARIQEIETGMWRRRGLHANDPRTIDIDII
                     YTGNLVISQENLVIPHPRWQEREFVVRPLADARPDLKLPGAKMTVRELLETFGGSENA
                     ILVSEDW"
     gene            complement(6154..6660)
                     /locus_tag="PKK36_05390"
     CDS             complement(6154..6660)
                     /locus_tag="PKK36_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNR94027.1"
                     /translation="MDVSEQQRQFPLDVDRVTGILNQISLLGGLTDEQLRRIFPLLGS
                     SRHEKGSVIFEQGGRPTHIYIVQSGRIKMVVEVDGTAMELIEFGTGQCFGETSVIGIQ
                     PHSATALAVEDTELIILSRDALHSLYKTDKDIFGMVVLNIAREACRRLYHTDEILLHY
                     ALSRNGGR"
     gene            complement(6667..7470)
                     /gene="dapB"
                     /locus_tag="PKK36_05395"
     CDS             complement(6667..7470)
                     /gene="dapB"
                     /locus_tag="PKK36_05395"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNR94028.1"
                     /translation="MTTRVAIIGAGGRMGQAMIRAIQTGAVRDLSISAAVERPDFGAL
                     GSDAGAAGKLGVCFTADVAAAAGASDAMIDFSFHTNTAEHARIASQSGVALVVGTTGM
                     SADEEGMVREAAKKIPVVFSANMSLGMNLLFSLVRQASKALADKGYDVEIIERHHRRK
                     KDAPSGSAVSLAKAAADGLGWDLNRVAVHGRSGLVGERPGEQIGIHAVRGGDIVGDHT
                     VLFAAEGECLELSHRATSRETFAMGALRAAAWASGRKPGLYSMKDVLEL"
     gene            complement(7481..8347)
                     /gene="dapA"
                     /locus_tag="PKK36_05400"
     CDS             complement(7481..8347)
                     /gene="dapA"
                     /locus_tag="PKK36_05400"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNR94029.1"
                     /translation="MFEGTYTAIITPFRRDGSVDYEKLDQLVEMQIEGGVTGIVPVGT
                     TGESPTLNTEEHIEVIRAVAMRCRGRAKVIAGTGANSTSEAVELTEAVREMGVDATLQ
                     VTPYYNKPNQAGLIAHFSAVADIGLPVVLYNVPGRSSREIAVETIVELSRHPNIVCVK
                     EAGGSVDRVSHIRRLCDIEILSGDDSLTLPMMAVGATGVISVATNVAPEPVAAMVADA
                     LAGRWEEARKLHLKYHRLFCDLFLDTNPIPVKAALAMMGLIEEVYRLPLCPMSDALKK
                     KLADCLREVNLI"
     gene            8622..9908
                     /gene="eno"
                     /locus_tag="PKK36_05405"
     CDS             8622..9908
                     /gene="eno"
                     /locus_tag="PKK36_05405"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR94030.1"
                     /translation="MSEIVDVLAREILDSRGNPTIEVEVQLASGATGTAAVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVENVNDVLYPELVGFDALDQVGLDKLMIELDGTET
                     KSKLGANAMLGVSLAAAKAAAQLVGVPLFRYIGGTNAKVLPVPMMNVLNGGAHSDAPV
                     DVQEFMICPKGAPSLTEAVRMGAEVFHALKSVLKKQGLSTAVGDEGGFAPNLPSNVAA
                     LDAIMEAIGLAGYKAGKDIFICLDPAASEFYDTKKKRYIFKKSDKSSKTAEQMVDFWA
                     KWVKDYPIISIEDGMAEGDWEGWKLMTDKLGKKIQLVGDDLFVTNTKFLKKGIELGVA
                     NSILIKVNQIGTLTETLDAIEMAKRAGYTSVISHRSGETEDSTIADIAVATNAGQIKT
                     GSLSRTDRICKYNQLIRIEEMLGEDAIYGGQVYGDR"
     gene            9976..10284
                     /locus_tag="PKK36_05410"
     CDS             9976..10284
                     /locus_tag="PKK36_05410"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNR94031.1"
                     /translation="MISWDAVYKGAWVVLIALLIAGSVLMFIPKLQEYRRYQQTAAKL
                     EEESRHEEEIIKHLKMQQERFRTDPRFVEFVAHEMGMVKSNETIFTFIDDLPATGQTR
                     "
     gene            10281..10472
                     /locus_tag="PKK36_05415"
     CDS             10281..10472
                     /locus_tag="PKK36_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94032.1"
                     /translation="MKIDPARDFGTRECPACACDVPANSNRCPICGYLFPNPSPARRR
                     FKIAAALIMLALLLALLLL"
     gene            complement(10479..11600)
                     /locus_tag="PKK36_05420"
     CDS             complement(10479..11600)
                     /locus_tag="PKK36_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNR94033.1"
                     /translation="MDKLSKYIATDFLVTFFMTLLIFTFVMCVGAMIKAIDLLARGVS
                     GDIILRVFLQNIPYILTFSIPISTLTASLLIFGRLSLDGELMAMKASGLTMWQIVCPV
                     VLISIALSGLCLYLNNHAAPRAHFGQRQMLREVGVEEPVNLLEEGRFVQDFPGMMIYV
                     GKKDRNKVKDVIVYEMDRYGVRRNVRAQTGEIRADEANKMLLIDLYDVRIDQPDRDDP
                     MDLVKTRHVTARHYPVQLDFEEIWKKDSIRKKMSDMTGPELKQAIRNIREAFPDLKEK
                     DVGKQRMKMLVTANKRLSLSLSCFAFTLIGIPLGISSKRKESSVGVGISLLLIFIYYL
                     FIIIADSLVGHPALHPDFIVWIPIIAAEAGGFLMIHRMN"
     gene            complement(11593..12492)
                     /gene="folD"
                     /locus_tag="PKK36_05425"
     CDS             complement(11593..12492)
                     /gene="folD"
                     /locus_tag="PKK36_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNR94034.1"
                     /translation="MTAKIIDGKAIARTMREEMAEEIARLKQRGIHPGLGVLLVGDDP
                     ASCSYVSAKEKACGEAGIHSRQIHLPASAPYDEILAATHDLNSDAAIHGVLVQLPLPD
                     PKMERGVLQAVSPDKDVDGLHPVSAGRLMLGLPAPWPCTPHGILQLLKRSRVKTAGAH
                     VVIVGRSSLVGRPLANLLSMKSEQGNATVTLCHSCTKDIAYHTRQADIVIAAAGRPKL
                     ITADMIREGAVVIDVGVNRVEDASAKKGYRLCGDTDFEGLREKASLITPVPGGVGPMT
                     ITMLIYNTVQSAAGAWSGGGAHG"
     gene            complement(12550..13836)
                     /locus_tag="PKK36_05430"
     CDS             complement(12550..13836)
                     /locus_tag="PKK36_05430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR94035.1"
                     /translation="MKLNTQETRLDNGLRIVSSHMPHAASVAMGIWVGVGGRYENQSM
                     QGMSHFLEHLLFKGTETRSARAISQEIEGRGGYLNAFTQEEATCYYARIGAKYVWPMA
                     NVLADMYLHPRFAPSDIEKERGVIIEEIMMYRDQPQQVAEDLLGEMVWEKHPLGRPLI
                     GTPKHILGYSRDDIAGFKRSKYCPANTVIALAGCVDHEAAVRRIAPLFEGLSAVRRPA
                     CRAVEPATPQRPFAQIEKKIEQSHLALALRLFGREDKRRYALKLLSVMLGENMSSRLF
                     QVVRERHGMAYSVHSSVHLFSDTGVLSVTAGLDRKKTAKAIALILKELDAMRRQEVSA
                     RELRRAKDYSIGQLQLSMESSSSQMMWIGGQILQRGCYEQPEEIIRKLEAVSAADVLR
                     LAREIIRASHFSLALVCPEGGAADPGWIKTETARLG"
     gene            complement(13836..14582)
                     /gene="hisA"
                     /locus_tag="PKK36_05435"
     CDS             complement(13836..14582)
                     /gene="hisA"
                     /locus_tag="PKK36_05435"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNR94036.1"
                     /translation="MTDITIIPAIDMKGGRCVRLRQGRAENETVYSDDPLAVARDWEA
                     QGAEFLHLVDLDGAFQGKPVHDGVILEIARTLQIPVEIGGGLRTREQIRRYIDGGVRR
                     VILGTRACESLDELKSLTAEFGDGIVVGIDARDGRVLVKGWVESSGIESLELARRVEA
                     CGVKTIIYTDISRDGMMKGVNADAMAAMCAAVSCNVIASGGVSSVADVKRLRKLDCGN
                     LTGAIVGKALYDKTVTLSELIEASGRRIKS"
     gene            complement(14579..15199)
                     /gene="hisH"
                     /locus_tag="PKK36_05440"
     CDS             complement(14579..15199)
                     /gene="hisH"
                     /locus_tag="PKK36_05440"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNR94037.1"
                     /translation="MIGIIDYGMGNLGSVGNACRFIGAPAKIVTTPGDLSGCGAMILP
                     GVGAFGDCVSHMTRHGFIDPVRDWIAAKKPFLGICLGLQMLFEGSEESPGVPGLGVFR
                     GQAKKFRLGSDFKVPQIGWNQVQQRQPACPLFAGVPDNSFFYFVHSFYVEPEDAAITA
                     GTTDYGMTYTSAVWKDNVMAVQFHPEKSHDTGLRMLKNFAELGTGS"
     gene            complement(15208..15798)
                     /gene="hisB"
                     /locus_tag="PKK36_05445"
     CDS             complement(15208..15798)
                     /gene="hisB"
                     /locus_tag="PKK36_05445"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNR94038.1"
                     /translation="MKSRKSVGKRKTKETDILVEVNLDGTGKYSVDTGIGFLDHMLEL
                     WSKHSLIDLKLKARGDLKVDYHHTVEDVGLVLGEALNQALRERKGIRRYGWSYMPMDD
                     TLSRVVVDLGGRPYLVYNINNPKKKIRDFDVGLIEEFFRALVTQARMNLHIAQLYGKD
                     VHHAYESVFKGFAKAMRMACEMDPRATGVPSSKGVL"
     gene            15861..16547
                     /locus_tag="PKK36_05450"
     CDS             15861..16547
                     /locus_tag="PKK36_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94039.1"
                     /translation="MTNTPHFIVRPAAGWYHGRMLEEIYSGMQDEDIPHYVDVFIDEA
                     ESRINDFLDEFVRRPVSRFLPSDHRLVFHALQRIRREQLAPGNRFCEWGCGFGVATGI
                     AAMLGFEAFGIEIDPRLVSRAGQLMKDFELAPTLCDGSYIPQGFDFYTDGVGQSMQLI
                     RAASDENARGWYRDWNFSVESFDVIFVYPWPSEEEFVEQLFDATAAEGALLVIYRGLD
                     DISIKRRVAT"
     gene            complement(16525..17172)
                     /locus_tag="PKK36_05455"
     CDS             complement(16525..17172)
                     /locus_tag="PKK36_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNR94040.1"
                     /translation="MGTKPLIPMHGGYRKLISFQVAQLAFDVTVRFCDRYIEKRSRTR
                     DQMVQAARSGVQNIAEGSQASGTSKKTELKLTNVARASLEELRLDYEDYLRQHGLPVW
                     DRDDPRRQELVDLRCTTADDVIRWIKAVHDRGQSGQNGQDHKGEKSTASTSSMISGIA
                     ANAVLILIAVACSLLDRQIASLAASFENEGGFTERLYRVRSEKRAAASVTSPRGA"
BASE COUNT         3648 a         5433 c         4745 g         3545 t
ORIGIN      
        1 cgatactgca ccgcgcgaaa gggcgccttg aagacaatcc cgttcagcgg ccgcgcgatg
       61 acgttctggt aataccatcc gccgttgaag tagtcctcca gccccgtgcc ccgccactgc
      121 ggctcagggg cggaatccat gcgcatcgtt tcgtcactct ccagcagcca ccagctccgg
      181 tcaaagctgg tcgcacccag cagacatgcc gcgaattttc ctgttccgcc ggtccgaagc
      241 acgttgtgcg gacgccccat catttcctcg ccgcttttcc tccacgcggc atgaaagtat
      301 ccatagtgcg ccggccagga aggcagcttc tccacgaccg cctccatcgt caggggcacg
      361 tcccaactgc tgaggttctg tatttcgatt tttgccgact gcgcgaacgg catcgggaaa
      421 cgaatgctga aaacgccgtc gcggtatccg aaaaacaggg aatcatactt caggcgctgg
      481 cccatgcttc cgaaaaaatc tccgagcgga acattcaccg agggactgct cccatcccat
      541 gaaatgcgca gtatcatgtc acgcagcagc tgccggcgcg caacggctga accaatcgaa
      601 gagtcatcca gacttactct gaactcgcga atcacggcag gccccggcag ttcgcagact
      661 gctgtgtccg acttctgttt gcagatcgcc tcatgccttt caccaggcac attggctccg
      721 ggagtactct tccatctcgc ccccagttca gaaagagccg tcttgtgcgc atcggtcata
      781 tcccggggcc agctctggat ggtttctcct tcgttcaaac gggaccagtt gatctgaaag
      841 aacagccttc gctcatcgcc gggcttcccg ccctcggtct gcttcgctcc tccgtcctga
      901 accatcaccg tcagtttttt tctgaagggg atcggaaccc acgaatacca gcagtagttt
      961 tcaaaacagg ccagcggggc aataaagggc tcggttcccc cgcagaaatc accaatggtt
     1021 gtatcaatgc ggggcttgcg ttctccgtca aaatacatgc gcaggccatg cgatccgtca
     1081 agcgctccgg taaaccagaa acgcgacata tatccaggcc cctccagatc cgccagcacc
     1141 acccacccgt ctggccccct gcgggcaaaa ttgttgaagt catcgtttcc gccccggcgg
     1201 tcataggagg taacgatctg tgtcgggcgg acatccagac gcgccattcg atccgtatcc
     1261 ttcaattcat tgaccagatc ggcccagagg atttcccgtc cttccggccg cgaacatccc
     1321 gccgccgcga ttaaaagcag caggccaatg actcctggga gacagatagt tttcatatgg
     1381 ccgcaatcta tgcaattcgc cccctgaagt caaaccgccc tcaggaagca gggtttctag
     1441 cgcttctccg cccactctaa ccagtcggag ggtttttggg aagccggtcc gtcctccacc
     1501 atcagtgaga gggcatgcgc acacatgtgc cccagccccg aggtatcccc cagcagtgcc
     1561 accagcggat caatggcctg cggatcacct atcattccga ggccgcgcgc cgcctgctga
     1621 cgcacctgac catactcggg atcggtgctg ttgagaagtt caagcaaatg aggaacagcc
     1681 tccctggact tcagcttccc aagcgccaga catgccgggg cagcggcata acgatcgctc
     1741 aaagccccaa tcagtgcagg aactgctgcg gggaatggat tgttttcgac cgcttttgcc
     1801 gcttccagtt tggggcccat ctccggatcc tgcagcagcc ggaccagaac acctcgcgaa
     1861 gcttcggtat taagccgtcc ggcagcaaac gcggcgcccg cacggacccc ggcgtcactg
     1921 tcgcgggcca cttgttccac aacagccatg gcctctggaa aaccaagttt tcccagcgac
     1981 cacacagcgg cccgtcgatc cgccgccgcc ctgcccgatc gcgcgattcg ggccatgacc
     2041 gcgacagaag agggacttcc cgccttttca agcgcatcca gtataaccgc tgcagaagct
     2101 tttggcggag tggaggactg agtctgcaaa agcatctgtt caagtttctg aatacccctg
     2161 atgtccccgc atgcggctaa acccagcagc gccggattgc gcttcttgta gtccgcgtca
     2221 tccgacagag aagcatgcgc atcataggtg ccgtctggag acagcccctg ttcgaatgcc
     2281 tccatttttt ccgcactgac cagactctga taatccacat cctcctgcac aggctgctcc
     2341 atgacagcat ccgcaacggg ggtactcggc cgcagttcca ctggctcatt aacagggaac
     2401 ccggcaggag ctgtcccgtc atcacgcagg atctgcgtga catcctttcg ccggattctt
     2461 gtaaaattct cacctgcaaa gggcagtttg atctctatat attccgcatc tgcggatatg
     2521 acttccccat caatcacacg cccgtttttc aggtgaatgg tatcggaaca agcaacctgc
     2581 acaaaaccca tcaggatcag aggtgcagtg tggatcaact tgtttgtcat tcgatgcgcc
     2641 tcctgtgttc aaatcaacaa cagcacagtc tagaccgcat attccggcaa tgcaagcatt
     2701 cgagcgccgg cgttgacacc tcccgtgcga acaggtactt taggcccatc atgagtactt
     2761 caaagaaaca aatactggtc acgggcggcg caggctatct gggttccatc atggtgccgg
     2821 aacttctgcg gctcggtcac agcgtcaccg tagtggacaa tttcatgtat cagcaggcat
     2881 ccctgctcga ctgctgcgcg gacaagaagc tgcaagtcat ccgcggcgat gcgcgcgatg
     2941 aggcgctgat gaaaaaggct gtcgccgggg cggatgtcat cattcccctc gcctgcctgg
     3001 tcggggctcc gctgtgcagc cagaagccca ttgaagcgcg caccatcaac ctcgacgcca
     3061 tcaagatgct ggtggatatg tccagcaaag accagatgat catatccccc accaccaaca
     3121 gcggttacgg gatcggcgaa aaggggaaat tctgcaccga aacaaccccc atgcgcccgg
     3181 tgtctctcta cgggaaactg aaagtcgagc ttgaggagta tctgctggcc agcgggcgtg
     3241 caatatccct ccgcctggcc acggtgtttg gcatcagccc ccgcccccgt ctggacctgc
     3301 tggtgaacga tttcactttc cgcgcggtaa acgaccggtt catcgtgctc ttcgaggcgc
     3361 acttcaagcg caattatatc catgtccgcg atgttgcagg ggcatttatc catgccctcg
     3421 acaattttga gacgatgaaa aacgagccct acaacgtcgg gctcagcgac gctaaccttt
     3481 caaaaatgga actgtgcctt gagatcagga aacagattcc cgatttctgc atcatggaat
     3541 ccgaagtcgg agaggatccc gacaagcgcg actacattgt cagcaacgac aaaattgagg
     3601 cgacgggatt caaaccgaaa cattcccttc aggacggaat cactgagctg atcaaaggct
     3661 tccagatcat ccgccggaac cagtattcga acatctgacc ggcgcactgt gaactgcttc
     3721 acggccagca aagacggcgt tgtgatcagc gtcaaaatca ttccaaatgc acccaggtgc
     3781 aaagtggatg gccttcttgg cgattacctc aaaatccgca ttcaggcgcc gcccgttgac
     3841 ggcaaggcaa acgcggctct gctcaaattc ctcgcaaaag aactgggagt tcccgtttcg
     3901 catctgaccg tcctgtccgg ccacaccgga cgcatcaaac ggatcgctgt atccggagtg
     3961 aataaacagc aggcggaatg ttcactggcg cctggaactt gagtttcagc ccagtatcgt
     4021 attgtcaatc aggcggggct tgcccaccca tacggccaga gccacgaggg atttaccgtc
     4081 gagggtctcc gttttctgca gggtttccca tttcacaacc tcgacataat caattcgcgc
     4141 ggcgggatat ttttcgatgt gggcccgcat ggccttcttc acagcggagg cggaacgctc
     4201 cccgccggca tacattttct ccgcgatgtc cagcgcctgc cgcaggcaca aggcctgcag
     4261 ccgttcttcc ccggttagaa acacattccg tgaactcatg gccagcccgt cgggctcccg
     4321 gaccgtcgga cccggaacaa tctccagcgg gaaattcata tccttcacca tctgctgcag
     4381 aatgcgaagc tgctgggcat ccttctgccc gaacaccgcg acatccggca tgactatgtt
     4441 gaacagtttg gccaccacgg tcagcactcc ccggaaatgg ccttcgcgcg tcgccccgca
     4501 aagctcacgg ctcaaaaaat cattgttcac atacgtggtt tcgccgggcg gatacatttc
     4561 ctcaaccgga ggatgaaata ttatatccac gccctccgct tcgcacatct tccggtcacg
     4621 ctcgaaatcg cggggatacg cggccagatc agcggccaca ttgaactgct tggggttcac
     4681 aaaaatgctg accgcgacaa catcagcacg ctccttcgca gttcgcatga gcgacaaatg
     4741 ggcctcgtgc aagtatccca tcgtcggcac aaaaccaatt cttcttccct gctggcgcag
     4801 ttcagacgcg aagtgctgca tctccaatat ggactcaatg atcctcatca atagccatgc
     4861 tcttcggtcg ggaatcggcc ctgctcgact tcctccctgt aaccggtcag ggcgcgggag
     4921 atttcagtgc cgagttctgc gtaacgcttc acaaagcgcg gttgctgtcc gtggtaaagt
     4981 cccagcaaat cgtgccagac cagcacctgc ccgtcgcacc cgggcccggc gccgattcca
     5041 atggttggaa tgcttatcga agcggtaata tctgccgcca gtttctgcgg aatgcattcc
     5101 agcaccagcg agaacgcgcc ggcatcggca agatcctttg catcctttct caaccgctcc
     5161 gcggcctgtt ctgttttgcc ctggacttta aatcctccaa aggcgttcac actctgcggc
     5221 gtcaggccca gatgcgcctg caccggaatc ccgttggccg ccatgcggcg gaccagttcg
     5281 acgcgcaccg cgccgccctc gacctttacg gcatcggctc cggcctcttt caggaagcga
     5341 ccggcattga tcagcgcctc gtcttcggag ccctgatagg acagaaacgg catgtccgcc
     5401 accaccatgg cctgccgcac cgcacgcgag acagcccgcg tatgatgcag catttcatcc
     5461 atggtaaccg gcagggtgct ttcgaagccc agcacggtca tgcccagcga atcacccaca
     5521 agaatgaggg gaattcccgc ctcgtcggcg agccgggcag acgaataatc ataggccgtc
     5581 acgcaggcga ttttcttctg ccccttctga gccctgatcg taagcactgt ccatggcttg
     5641 ttcatcgtca ccagtcttcc gaaaccagta tggcgttttc acttccgcca aacgtttcca
     5701 gcagttcccg aactgtcatt ttcgcgccgg gcagtttcag gtccggccgc gcatccgcca
     5761 gcgggcgcac gacgaattcc cgttcctgcc agcggggatg cggaatcaca agattctcct
     5821 ggctgatcac cagattcccc gtataaatga tgtcgatatc aatcgtgcgc ggatcgttgg
     5881 cgtgcaggcc gcgccgccgc cacatgcccg tttcaatttc ctgtatccgc gccagccatt
     5941 ccagcggagt tcgtacactt tccagaatca caaccacatt caggaaagcc atgttcttgt
     6001 agcagtcgcg cacgtccaca ggacgggtct cgtaaaggcg ggatttggcc agaatctcca
     6061 catcgcctcc ttctgtcagc gccagcagcg cccgatgcag aaaatcctga cggtcgccga
     6121 tgttgctgcc catgctgaat cccgcttcca tagctatctc cctccgttcc tgctgagcgc
     6181 atagtgcagg agtatttcat ccgtgtgata cagcctccgg caggcttcgc gcgcaatgtt
     6241 caaaaccacc atcccgaata tgtccttgtc ggttttgtag agcgaatgga gcgcatcccg
     6301 ggagagaatg atcagctccg tgtcctccac ggccagcgcg gtggcggaat gcggctgaat
     6361 gccgatgacc gatgtttcgc caaagcactg gcccgtgccg aattcaatca gctccatcgc
     6421 ggtgccgtca acttccacca ccattttgat gcgtcccgac tgcacgatat agatatgagt
     6481 gggccggccg ccctgctcga agatgacgga tcccttctca tgccggctcg accccagcag
     6541 cggaaaaatc cggcgcagct gttcatcggt cagtccgccc agaagggata tctgattgag
     6601 gatacccgtc acacggtcca catcaagcgg gaactgccgc tgctgttcag aaacgtccat
     6661 gcctgctcaa agctccagca catccttcat ggaataaaga cccggcttgc gcccggatgc
     6721 ccatgcggcc gcacgcagcg cgcccattgc gaaggtttcg cggctcgtgg cccggtgaga
     6781 aagctccagg cattccccct ctgccgcgaa cagaacggtg tggtccccca caatgtcccc
     6841 gccgcgcacg gcgtgaatgc cgatctgctc gcccgggcgc tcgccgacga ggccggagcg
     6901 tccgtgcacg gccacgcggt tgagatccca tcccagaccg tccgcggccg ctttcgccag
     6961 gcttacggcc gaaccgctcg gcgcgtcttt tttgcggcgg tggtgccgtt cgatgatttc
     7021 cacgtcatag cccttgtccg caagcgcttt ggacgcctgc cggacgagcg agaaaagcag
     7081 gttcattcca aggctcatgt tggccgaaaa cacaaccgga atcttctttg cggcctcacg
     7141 gaccatgccc tcttcgtcgg ccgacatgcc ggtcgttccc accacaaggg ccactcccga
     7201 ctgcgaggca atccgcgcat gctcggcggt gttcgtgtga aaactgaaat caatcatcgc
     7261 atccgaggct ccggccgccg ccgccacgtc cgctgtgaag cagaccccga gtttccccgc
     7321 ggcgccggca tcgctgccga gcgccccgaa atcgggccgc tccacggcgg cggaaatgga
     7381 aagatcccgc accgcgccgg tctggatcgc gcggatcatg gcctggccca tgcgccctcc
     7441 cgccccaata attgccactc tcgttgtcat agcgcctcct tcagatcagg ttcacttccc
     7501 gcaggcagtc cgccagcttc ttcttcaagg catcgctcat cggacagagc ggaagacggt
     7561 acacctcttc aatcagtccc atcatcgcga gcgcggcctt caccggaatg ggattcgtgt
     7621 ccaggaacag gtcgcagaac aggcgatgat acttcaggtg aagcttccgg gcctcttccc
     7681 agcgcccggc cagcgcatcg gccaccatgg ccgcaacggg ctccggggcc acattcgtgg
     7741 ccacactgat tacgcccgtg gccccgacgg ccatcattgg aagcgtcagg gaatcatcgc
     7801 cggacagaat ctcaatgtcg cacagcctgc ggatatgact cacgcggtcc acgctgccgc
     7861 cggcctcctt cacgcacacg atgttcgggt gccttgaaag ctcaacgatc gtctcgacgg
     7921 caatctcgcg cgacgagcgg ccggggacat tataaagcac cacgggcagg ccgatatcgg
     7981 ccaccgccga aaaatgcgca atgaggcccg cctgattggg cttgttgtag tacggcgtca
     8041 cctgcagcgt ggcgtccacg cccatctccc tgacggcctc ggtcagctca acggcctccg
     8101 acgtggaatt ggcgcccgtg ccggcgatga ccttcgcgcg gccccggcag cgcatggcca
     8161 cggcgcggat cacctcgata tgctcctcgg tgttgagcgt gggcgactcg ccggtggtgc
     8221 ccaccggcac aatgccggtc acgccgcctt caatctgcat ctccaccagc tgatccagct
     8281 tctcgtaatc caccgagccg tcccttcgga acggcgttat aatcgcggta tatgtccctt
     8341 caaacatggc ttgcctccgt cggccttttc aaaaagttcc aaccattgga agttttttcg
     8401 ggaaaagttc caaccattgg aagttttccc caaaatagtt ccagtcattg gaaaatctaa
     8461 acacgatttt tccagtgatt ggaagttttt acgcatattt ttcagcggct ggaacccttc
     8521 cggggccgca ttttggacat tttcgccttt gcaagcaggg tcggtttggc tatcgtcgcg
     8581 gttcgtttgg gtgatatgca aactttttaa ggagaatgct catgtcagag attgttgatg
     8641 tacttgcaag agagatactg gattcccgcg ggaatcccac gattgaagtg gaagtccagc
     8701 tggcttccgg cgccaccggc acggcggcgg ttccctccgg cgcctcgacc ggcgagaacg
     8761 aggccattga actgcgcgac ggcgacaaga aacgctatct cggcaagggc gttctgaagg
     8821 ccgtcgagaa cgtgaatgat gtgctttatc cggaactcgt cggttttgac gccctggacc
     8881 aggtcggact ggacaagctg atgattgagc tggacggaac tgagaccaag agcaagctgg
     8941 gcgcgaatgc gatgctgggt gtttcgctgg ccgccgcgaa ggccgccgcg cagctcgtcg
     9001 gcgtgccgct gttccgctat atcggcggca ccaacgcgaa ggtccttccg gtcccgatga
     9061 tgaacgtgct caacggcggc gctcattccg acgcgccggt ggacgtgcag gaattcatga
     9121 tctgcccgaa aggcgccccc tccctcaccg aggccgtccg catgggggcc gaggtgttcc
     9181 acgcgctgaa gagtgtgctg aaaaagcagg gcctgagcac ggccgtcggc gacgagggcg
     9241 gcttcgcccc caacctgccg agcaatgtcg ccgcgctcga cgcgatcatg gaggccatcg
     9301 gcctcgcggg ctacaaggcg ggcaaggata tcttcatctg cctggacccc gcagccagcg
     9361 aattctatga caccaagaaa aaacgctaca tcttcaagaa gagcgacaag tccagcaaga
     9421 ccgctgaaca gatggtggac ttctgggcga agtgggtgaa ggactatccc atcatcagca
     9481 tcgaggacgg catggccgag ggcgactggg aaggctggaa gctgatgacc gacaagctcg
     9541 ggaaaaagat ccagctggtg ggcgatgatc tgttcgtcac caacacaaag ttcctgaaga
     9601 agggcattga gctgggcgtc gccaactcga tcctgatcaa ggtcaaccag atcggcaccc
     9661 tgaccgagac gctggatgcc atcgaaatgg ccaagcgcgc gggctacacc tcggtgatca
     9721 gccaccgttc gggcgaaacc gaggacagca ccatcgcgga tatcgccgtg gcgaccaacg
     9781 ccgggcagat caagacgggc tcgctgagcc ggacggaccg catctgcaaa tacaaccagc
     9841 tgatccgcat tgaggaaatg ctgggcgaag acgccatcta cggcggccag gtgtacggcg
     9901 accgttaggt tgaagaagcc atgaatcaga acggcgccgc accgcagggc ggcgccgttt
     9961 tgactggaag cggacatgat ttcgtgggat gcagtttaca aaggggcctg ggtcgtgctg
    10021 atcgcgctgc tgatcgccgg gagcgtgctc atgttcattc cgaaactgca ggagtaccgg
    10081 agataccagc agacggccgc aaagctggag gaggaatccc ggcatgaaga ggaaatcatc
    10141 aagcacctga agatgcagca ggagcgcttc cggaccgatc cccgctttgt ggaatttgtg
    10201 gcgcacgaga tgggcatggt caagagcaac gagaccattt tcaccttcat tgatgatctt
    10261 cccgccacag gtcagacacg gtgaagattg atccggcaag ggattttgga acaagggaat
    10321 gcccggcctg cgcgtgtgat gtccccgcca acagcaaccg ctgcccgatc tgcggctatc
    10381 tcttcccgaa tccttcgccg gcgcggcgca ggttcaaaat cgccgcagcc ctgatcatgc
    10441 tggcgctgct gcttgccctg cttctgctct agccggcttc agttcatccg gtggatcatc
    10501 aggaacccgc ccgcctcggc cgcgatgatg ggaatccaga caatgaaatc cggatgcagg
    10561 gccgggtgcc ccaccagcga gtccgcaatg atgataaaca aatagtagat gaaaatcagc
    10621 agcaggctga tgcccacccc gacagacgat tccttgcgtt tggagctgat ccccagcgga
    10681 atgccgatca gcgtgaaggc aaagcatgac agcgagagcg acaggcgctt gttggccgtc
    10741 accagcattt tcatccgctg cttgccgacg tccttttcct tgaggtccgg gaatgcctcc
    10801 cgaatgttgc gaatcgcctg tttcagttcc gggccggtca tgtcggacat tttcttgcgg
    10861 atggaatcct tcttccaaat ctcctcgaaa tcgagctgaa ccggataatg ccgcgcggtg
    10921 acgtgccggg ttttcacgag atccatcgga tcatcccggt ccggctggtc aatgcgcacg
    10981 tcatagagat caatcagaag catcttgttg gcctcgtccg cgcggatttc gcctgtctgg
    11041 gcgcgcacat tgcgccggac cccgtaccgg tccatctcgt agacaatgac gtccttgacc
    11101 ttgttgcggt cctttttccc gacatagatc atcatgccgg ggaaatcctg cacaaagcgt
    11161 ccttcctcca gcaggttgac cggctcctca acgcccactt cccgaagcat ctgccgctgg
    11221 ccgaagtgcg cacgaggggc ggcgtggttg ttcagataga ggcacaggcc cgaaagcgcg
    11281 atggagatca gaaccaccgg gcagacaatc tgccacatcg tcagcccgga cgccttcatg
    11341 gccatcagtt ctccgtcaag cgaaagtcgc ccgaagatca gcagcgaggc agtcagcgtg
    11401 ctgatgggaa tcgaaaaagt caggatatag ggaatgttct gcaggaacac cctcaggata
    11461 atatcccccg acacgccgcg cgcaagcaga tcgatcgcct tgatcattgc ccccacgcac
    11521 atgacgaacg tgaagatcag gagcgtcatg aaaaaagtaa ccagaaaatc ggtcgcgatg
    11581 tatttgctga gtttatccat gcgcgccgcc cccgctccag gcgccggccg ccgattgaac
    11641 ggtgttgtag atcagcatgg tgatggtcat gggccccacc ccgcccggca ccggcgtgat
    11701 cagcgaagcc ttctcccgga ggccttcaaa atcggtgtcg ccgcaaaggc ggtatccttt
    11761 ctttgcggag gcatcctcca cgcgattgac cccaacatca atcacgaccg cgccctcgcg
    11821 aatcatatcc gccgtgatta gtttcgggcg gcctgcggcc gcaatgacaa tatccgcctg
    11881 ccgcgtgtgg tatgcgatgt cctttgtaca ggaatgacaa agcgtgactg tcgcgttgcc
    11941 ctgctctgat ttcatggaaa gcagattcgc aagcgggcgg cccacgaggc tgctgcggcc
    12001 gacaatcacc acatgcgcgc cggccgtctt cacacgtgaa cgcttgagaa gctgaaggat
    12061 tccatgcggc gtgcagggcc acggagccgg aagccccagc atgagccggc ccgcgctgac
    12121 cggatgcaga ccgtccacgt ccttgtccgg cgaaaccgcc tgcagcacac cccgctccat
    12181 ctttgggtcc ggaagcggaa gctggaccag caccccatga atcgcggcat cgctgttcag
    12241 atcatgggtc gcggccagaa tctcatcata gggggccgag gcaggcagat gaatctgtcg
    12301 tgaatgtatg ccggcctccc cgcaggcctt ttccttggcg gagacatagg agcaggaggc
    12361 cggatcgtcg cctaccagaa gcacaccaag ccccggatga atgccccgct gtttcaaccg
    12421 ggcaatctcc tcggccattt cttcacgcat ggtccgcgca atggcttttc cgtctatgat
    12481 ttttgccgtc atttcgaagg aggtatacta gaaggatgga ccaaaggact caagcccgca
    12541 ctgctctgat tatccgagcc gggcggtttc ggttttgatc cagccggggt ccgcggcgcc
    12601 gccctcggga cacacaaggg caaggctgaa atgactggcc cgtatgattt cgcgggccag
    12661 ccgcagaaca tcggcggccg aaaccgcctc cagtttccgg ataatctcct ctggctgctc
    12721 ataacacccc cgctgcagaa tctggccgcc gatccacatc atctggctgg aggagctttc
    12781 catgctgagc tgaagctggc cgatggaata atcctttgcc cgccgcagtt cccgcgcgga
    12841 tacttcctgc cggcgcatcg catccagctc cttcaggatc agcgcaatgg cctttgccgt
    12901 cttcttccgg tccaggccgg ccgtgacgga aagaacaccc gtatcgctga acagatgcac
    12961 ggagctgtga acggaatagg ccatgccgtg ccgttcccgc accacctgaa agaggcgaga
    13021 actcatgttc tcgcccagca tgaccgaaag aagcttcagc gcatagcggc gcttgtcctc
    13081 gcgcccgaaa agcctcaggg ccagcgcaag atgactctgt tcgattttct tctcgatctg
    13141 cgcaaaagga cgctgcggcg tggcgggttc aaccgcccgg caggccggac ggcggacggc
    13201 cgacaggccc tcaaaaagcg gggcgatcct gcggacagcc gcctcgtgat ccacgcatcc
    13261 ggccagcgca atcaccgtgt tggcgggaca gtattttgac cgcttgaatc cggcgatatc
    13321 atcgcgcgaa taaccgagga tatgtttcgg ggtgccgatc agcgggcggc cgagcggatg
    13381 cttttcccag accatctcgc ccagcaaatc ctccgcaacc tgctggggct ggtcgcggta
    13441 catcatgatc tcctcgatga tcactccgcg ctccttctca atgtcggagg gagcaaaccg
    13501 cggatggaga tacatgtccg caagcacatt ggccatgggc caaacatact tcgcgccgat
    13561 gcgcgcgtaa tagcaggtgg cctcctcctg tgtgaaggcg ttcaaatatc cgccgcgccc
    13621 ttcaatctcc tgcgaaatcg cgcgggcgct gcgggtctcc gtgcccttga aaagcagatg
    13681 ctccaggaaa tggctcatgc cctgcatgga ctggttttcg taccggccgc cgacccccac
    13741 ccagatgccc atcgccacgc tggcggcgtg gggcatgtgg ctgctgacaa tgcgcagccc
    13801 gttgtcgagc ctggtttcct gcgtgttgag cttcattatg attttattct ccgacccgag
    13861 gcctcgatta attcagaaag cgtcaccgtt ttgtcgtaaa gcgccttgcc aacaatcgcg
    13921 cccgtcaggt taccgcaatc gagtttacgc aaccgtttaa catccgccac ggaagacacg
    13981 ccgcccgaag cgatcacatt gcaggaaact gccgcgcaca tggccgccat cgcgtcggca
    14041 ttcacgccct tcatcatccc gtcgcgcgat atatcagtat aaatgatcgt tttgaccccg
    14101 catgcctcta cgcggcgggc cagttcaaga ctctcgatgc cggatgattc cacccagcct
    14161 ttcaccagca cacggccgtc gcgggcatct atgcccacga ctatgccgtc tccgaattca
    14221 gccgtgaggg atttcagttc atccagcgac tcgcaggcgc gggtgccaag gatcacgcgg
    14281 cgaacgccgc cgtcaatgta tctcctgatt tgctcgcggg tgcgcaggcc gcccccgatc
    14341 tcaacaggaa tctgcaaggt ccgggcgatt tcaagaatga ctccatcatg caccggttta
    14401 ccctgaaacg cgccatcaag atccacaagg tgcagaaact cagcaccctg cgcctcccag
    14461 tcgcgtgcga cggccagcgg gtcgtcggaa taaaccgttt cgttctccgc gcgcccctgc
    14521 cgcagacgca cgcagcgccc gcccttcata tctatcgccg gtatgatggt gatgtctgtc
    14581 atgatcccgt ccccagttcc gcgaagtttt tcagcatgcg caggccggtg tcatggcttt
    14641 tttccggatg gaactggacg gccatgacat tgtctttcca gactgccgat gtgtaggtca
    14701 tgccgtagtc cgtcgtgccg gcagtgatgg ctgcatcctc cggctcgaca taaaaagagt
    14761 gcacgaaata aaagaatgaa ttatccggca ctcccgcaaa cagcgggcag gccggctggc
    14821 gctgctgcac ctggttccat ccaatctgcg gtactttgaa gtcggagccc aaacggaact
    14881 tcttcgcctg tccccggaac acacccaggc cgggaacgcc gggcgattcc tcgctccctt
    14941 caaaaagcat ctgcagtccg aggcagattc cgagaaaagg ttttttcgcc gcaatccagt
    15001 ccctgacggg atcaatgaat ccgtgccggg tcatgtggct tacgcagtct ccgaaggcgc
    15061 ctactccggg cagaatcatc gcgccgcatc cggagaggtc tccgggcgtt gtgacgattt
    15121 ttgcgggggc cccgataaaa cggcaggcgt ttcccacgct gccgaggttt cccatgccgt
    15181 agtcaatgat gccgatcatg gcggatttca gagcacgccc ttgctggacg gcacacccgt
    15241 ggcgcgggga tccatctcgc aggccatgcg catggctttg gcaaaaccct tgaaaaccga
    15301 ctcataggcg tgatgcacgt ccttgccgta aagctgggcg atgtgcaggt tcatccgggc
    15361 ctgcgtgacc aatgcgcgga agaactcttc gatcagcccg acatcaaagt cgcggatttt
    15421 ctttttcgga ttattgatgt tatagaccag atagggccgc ccgcccagat ccaccaccac
    15481 gcggctcagg gtgtcgtcca tgggcatata gctccagccg tagcggcgga tgcccttgcg
    15541 ttcgcgcagc gcctggttca gcgcctcgcc cagcaccagc ccgacatcct cgactgtgtg
    15601 atggtaatcc accttaagat cgccgcgggc cttaagcttc aggtcaatca gcgaatgttt
    15661 gctccagagc tcgagcatgt ggtcaaggaa accgataccg gtgtccaccg agtacttccc
    15721 ggtgccgtcc agattcactt caaccaggat atccgtttcc ttcgtcttgc gttttccgac
    15781 tgacttgcgt gatttcatag ctgttcctcg aataaaacgt gtgcatcgta agccggaacc
    15841 gcccgcgata aaagccagaa atgacaaaca ctcctcattt tattgtgcgg ccggccgcgg
    15901 gctggtatca tggccgcatg ctggaagaga tatacagcgg aatgcaggat gaagacattc
    15961 cgcactatgt ggatgttttc attgatgagg cggaatcgcg catcaacgat ttcctggacg
    16021 agttcgtccg caggccggtt tcgcgttttc ttcccagcga tcaccggctg gtgtttcatg
    16081 cgctccagcg catccggaga gagcagctcg cgccgggcaa ccggttctgc gaatggggct
    16141 gcggattcgg agtggccacc gggatcgccg ccatgctggg atttgaagcc ttcggaattg
    16201 aaattgaccc gcggctggtt tcgcgcgcgg gacagctcat gaaggacttc gaactggcac
    16261 ccaccctctg cgacgggagc tatatcccgc agggattcga tttctatacc gacggcgtcg
    16321 gacagtccat gcagctcatc cgcgccgcat ccgatgaaaa cgcgcgcggc tggtaccgcg
    16381 actggaactt ctcggtcgaa agctttgatg tcatcttcgt ctatccctgg ccaagcgaag
    16441 aggaattcgt tgagcagcta tttgacgcca ccgccgcgga aggcgcgctg ctggtcatct
    16501 atcgcggact cgacgacatc tccatcaagc gccgcgtggc gacgtgacag aggccgccgc
    16561 gcgcttttcg gagcgcacgc gataaaggcg ttccgtgaat cctccttcat tctcaaagga
    16621 tgccgccagg gaagcaatct gccggtcaag caggctgcag gccactgcaa tcagaatcag
    16681 caccgcattg gcagcaattc cggaaatcat ggacgaggtg gacgcagtgg acttctctcc
    16741 cttatgatcc tgtccattct gtccactttg tccacgatca tggacagcct taatccagcg
    16801 aatgacgtca tcagccgtag tgcaccggag gtccaccagt tcctgccggc ggggatcgtc
    16861 gcggtcccag accggcagac cgtgctgacg cagataatcc tcatagtcga ggcgtaattc
    16921 ctccaggctg gcccgcgcca cattggtgag tttgagctcg gtcttctttg acgtgccgga
    16981 ggcctggctg ccctcggcga tattctgcac gcctgaacgg gcggcctgca ccatctggtc
    17041 gcgtgtgcgg cttcgctttt caatgtagcg gtcacaaaag cgaacggtca cgtcgaatgc
    17101 cagttgcgcc acctgaaaac ttatcagttt ccgatatccg ccatgcatcg gtataagtgg
    17161 cttcgtgccc ataattgctc ccatattgct gaattaatga aattctgcaa accaagaatc
    17221 gcatggcata aaatccgggc cgacccgccg tttttcataa aaatggctat tttttggacg
    17281 tttttgagcg ttttgagcaa taaatgagca tttttagcac ttctgtccca gtattaattc
    17341 cacagcatca actggttagg cggtgatagt g
//
LOCUS       DAOQWR010000047        16839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3837_length_16839_cov_5.345150, whole genome shotgun sequence.
ACCESSION   DAOQWR010000047 DAOQWR010000000
VERSION     DAOQWR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_16839_cov_5.345150"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..842
                     /locus_tag="PKK36_05460"
     CDS             147..842
                     /locus_tag="PKK36_05460"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNR94041.1"
                     /translation="MQSNTDTQVVAVIPARGGSKGIPGKNIRLLAGKPLLAWTVEAAV
                     AANSIDSVYVSTDDPAIAKVAEACGAEVIFRPADISGDESPSEAALLHALGYLNKQGM
                     VVEIMVFLQATSPLRTSADIDCAMALFQEQQADSLLSVAPSHYFLWEETKEGAQPVNY
                     DYCHRPRRQDMIQQFRENGAIYIFKPSILREYSSRLGGRIILYKMLEESSLDIDSYSD
                     FERAGAYLRGRKN"
     gene            845..3100
                     /locus_tag="PKK36_05465"
     CDS             845..3100
                     /locus_tag="PKK36_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNR94042.1"
                     /translation="MIITKNIFSYCISSDDSIYAALSKIDANAEGFVFCIDNAGVLEG
                     ILTDGDFRRWLMKAKYVDTSRPVRDIARQEFVAAPLLSSREKILRLFSKKIRFIPLLD
                     DRRRLVGIARRRNLAEGILIGDLLISDHAPVFVIAEIGINHNGSLPRAHRLIDAVRES
                     GANCVKFQMRNMHALYRNVAEADLTGEDLGPQYTLNLLSRFELLPEQMLELFDYSHQA
                     GLIPLCTPWESESLKILEAHDMAAYKVASADLTNHPFLEELARTYKPLIVSTGMSTEA
                     EIEQSVKLLKQLGVAYALLHCNSTYPVPFKDVNLRYMHRLKEMGDCVVGYSGHERGYS
                     VALAAVSMGAKIIEKHITEDKTLEGTDHKVSLLPQEFKAMVEGIREVELALGVGDVRQ
                     MSQGELMNRSNLAKSLIINCDLKEGEIIEPNMIEIKSPGRGLQPNYREELIGSVSKRN
                     MVKGDFFYPTDIQKEEINARKYTFSRSWGVTVRWHDFKDILCKTNPDFLEFHLSFKDM
                     DEDYMKYFDQVYDLDLKVHSPDTFAGDHLLDLSNPNPDHRKRSIRELQRVVDLTRNLK
                     QFFRRATRPMIICSLGGFSTDGLLIADKVKERYEIMEDSLTQIDASGVEIIGQTLPPF
                     PWYFGGQMYLNLFVHADDTVAFCEANQLRLCYDISHSKLTCNYFKTSFQEFTAKVAPY
                     VAHMHIADASGVDGEGLQIHEGEIDFPAFIAEVKAQCPDASFIPEIWQGHKNDGEGFW
                     VALEHLEAMKF"
     gene            3135..4508
                     /locus_tag="PKK36_05470"
     CDS             3135..4508
                     /locus_tag="PKK36_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94043.1"
                     /translation="MNESRTVENRIVIPIETKVREFQGKLLCALTLAERGYRVILGDQ
                     RILMSTLEQYTPCIYVDKSFASTKRLGYTKCKTLGNRLLAWDEEGLVYFDAQTYRDLR
                     LDANCLSMLDAAFAWGPDQAEILKGITPSGLPVIEIGHPRMDLLRPEFRGFYKTPVEK
                     LQSKYGKMLLVNTAFPLANHFHGDMQIQQKQELYPIAQHRPDFFEKWKKVQYAGMVSF
                     KEMIPELCRAFPEYTVVVRPHPSEKRGIWESFTSEINNAVVNADGNVVEWILASECVI
                     HFDCTSGLEAFLLEVPAAVYRRSASPGYEQQLPNSLSYRVQTCDEVLSFVRGVLAGQK
                     SRLLDDPERMALLRRHMVAADGELSCDRFADALDALALTRLPQKASEKIPSLNVAWRW
                     WLKRYRRMNLKHSAYAHQKFSGLSVQEIERVTESMAEALGRFSSVAIHQIDEAVFQIT
                     GFKDDVL"
     gene            4505..6109
                     /locus_tag="PKK36_05475"
     CDS             4505..6109
                     /locus_tag="PKK36_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNR94044.1"
                     /translation="MSEYIRTSYIAGISSSKHPARQLRMILFRWNIPAVVCRLLRRKY
                     SIIEIALVLCLVVLAWGSGSDLPDTQAVKSDARAYATLAVNLWRHGVFSSDEADTVSP
                     MPTQYREPAYPAFLALVIATHRELRNKPMENFLSAGKDIRSLKRSGLLLFVLLPVLIY
                     VLVRKAGGSRAAALSAGLLVIMDRGSQNLLATTLSEHLTMVAMAAVSLSLMGLVRRPG
                     TGCAALGGLALAVLVLTKTVFQLFLPVALLWVWWNGRGLTVSRRWIAVAVFAGAFALP
                     VGAWKLRNVAHFGNASLVSGRSGVVLMARALSDTMTPEERRAAWIYWMPEGKLRNRCA
                     RSAFGNRFEEATRRLDRRCPESFYRRARIERQTLLEEMGAGPRVHHEMDRRLTRYALN
                     IIQQHAGAHLALTPAFAWRCLFSTRGLRLLWPFTWKWEAPLRWTMLWAGGLLIGTVMS
                     FLRRDICVLALTLPSLFLLGSHAFLTHCVPRYGLGMVPFGIGALALVGDATTRRLAKR
                     YKRWKDQNPLRREIREETHHPDSLPE"
     gene            6072..7049
                     /locus_tag="PKK36_05480"
     CDS             6072..7049
                     /locus_tag="PKK36_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR94045.1"
                     /translation="MKKLIIQIPCLNEEEHIGHTLASLPRAVPGFDQVELLVIDDGSS
                     DNTVAVARAAGADHVVSLSSHQGLARAFIAGLDRAVELGADVIVNTDADNQYCADDIP
                     QLTLPVLEGRADMVVGERPIESIGHFSAAKKRLQRLGSWVVRAASGTQIPDAPSGFRA
                     LSADAARRLNIFSNYTYTLETIIQTGHKNMSVLSVPVRTNAFVRPSRLMKSTLRYVWH
                     SLITIVRIFVVYRPFRFFMTVGGVLFGIGLLIGIRFLYYFMIGGGEGKVQSLILASIL
                     LGIGFQNMLVAFMADLLSVNRRLIEDVQYRLRCGEVYPRASGEKEKCDV"
     gene            7042..7614
                     /locus_tag="PKK36_05485"
     CDS             7042..7614
                     /locus_tag="PKK36_05485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94046.1"
                     /translation="MSDQGAEGILSAWLRRHRLAATRPHLRGTVLDIGCGAGALADEY
                     PAEHYVGVDIDEESLAVARHHHPEHQFCAAADPELRVNTIALLAAIEHVADPAGFLRG
                     LANHLLPGGHFVLTTPAPWTDRVHAWGAAMGLFSHAAHEEHEKLIDPPYMRAISHSAG
                     LRVTYMRRFLFGANQLFILDRDPTKERSTL"
     gene            7647..8174
                     /locus_tag="PKK36_05490"
     CDS             7647..8174
                     /locus_tag="PKK36_05490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR94047.1"
                     /translation="MRGVQVVVGTALFDVWPLLSLRHAILRLYFQAGAGFLVGRHFQF
                     HRPHFLRAPAGARLTFAQNVKINSNVEIDYSGGVEIADDVWISQNVIIETHEHVIGSG
                     PKSGWLVKWFPLEIGRDAWIGANAIILPGVKRIGARAIVGAAAVVSREVLDGEIVGGV
                     PARMIGQRPAEREGE"
     gene            8175..9134
                     /locus_tag="PKK36_05495"
     CDS             8175..9134
                     /locus_tag="PKK36_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94048.1"
                     /translation="MGADGSHSAWRWIGRFLSLCGLLFVAWRAWPLRTDLAQALAGPG
                     VRTAFILAIGVFLLDNVILASIWGGLLRVCGATKFAMRTTLWLYAQTQIAKYLPGNIM
                     HFAGRHAAARSRGVAHAPLAAAAMMEFGGLIAAAGGVALIGLSLSAGDGQRTVMVRLA
                     VISLIVLLGLSALICLVPRTAVFRRLGGGVLVPIVVARGLSVAILGYVVFFLIGGMSC
                     ILLANALSGDGFHGSGSILFAYAVSWIMGFITPGAPGGLGVREAALSGLLTAQLGAAP
                     AVLAALLFRGVTVGADVLFYAGSFVWTPMNERRNLPCEERLGL"
     gene            9290..11023
                     /locus_tag="PKK36_05500"
     CDS             9290..11023
                     /locus_tag="PKK36_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94049.1"
                     /translation="MKHIIINMIEILVYFRQRYLRIGIILVICLVLMSVLEGVGIGLF
                     LPLFELIGKGSEVGTSPISQLVARLFAVFHLPLTLGPIVLFVIVMMLTKGLVRWLTMR
                     QVAWMTAQISVDMRLELFDALLRADWDYASSRAAGRFANAVGVETKISAHMFRHIIVT
                     IACFLQVFVYLLLALVVSWKVALLSILAGLFMLLLFHKLIMIAKMAGKQTVLLQRALV
                     IRLIDIVGGLKSMKAMGQEALVYPILKAKTEKLRKAEAQSMMTVETMSAFQEPLTMVM
                     LAIGAYVLLKFTAIEFAPFVMLALLFTRSVGQINQMITNYQAIAVKEGGFFELRKEVA
                     AARAHQEVGLLDEVSVDLSRSIALQGVTKKYADAEPVLENISLEIRRGQMIAFRGPSG
                     CGKTTMADIISGIIRPTSGQVLVDGEPLTDVRLKGWRHRIGYVPQEVLLLEDSVYANV
                     TLGDRRISREKCIEALKKANAWDFVSRLQDNIDYVVGRAGAGLSGGQRQRIAIARALV
                     RDPAVLVLDEVTRSLDRESEKSICESIAALKGSITIIAISHQEAFISLADELWEFEGS
                     GKVIVKKHLFK"
     gene            11020..11760
                     /locus_tag="PKK36_05505"
     CDS             11020..11760
                     /locus_tag="PKK36_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNR94050.1"
                     /translation="MNLLNKVLIRLLGRRLELIQVAEYPKCGGSWVARMIRSYVGVDR
                     KYGITAPVCRHAVIQTHDLYTSFYARNIVVIRDPRDVWVSFYFHERYLKRTAPVYDFD
                     DALSHAQNMLRCMRQKLSAPEKSMPGFSYADFLKSWEGRPATAHVKYEALHADAQGEL
                     SRLVRALDMEPESGRITAAIEANRFEKIAGREPGQEDFTKHKRKGIVGDWKNYFTREM
                     GDLVHQHQPFLYELGYEPDDSWLKELPQ"
     gene            11757..12476
                     /locus_tag="PKK36_05510"
     CDS             11757..12476
                     /locus_tag="PKK36_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94051.1"
                     /translation="MSPEFIEVFGIPASGKTMLVNQLCGTHGAVSREEALRGALTVKR
                     LGLVRWMLHRCGLSGGSFDEITCGLLEQSALANRRLVQTVFDYLVQAGVPDREAGVAL
                     RGIFRTAREYALLTEASADARILMDEGWIHRALTVFGMRASDEVPDEWLQRYADSVPA
                     PRFAIYVECDPETSFSRVSARERKARFWWGEWTEAEQRRALAQTARCAARIAEHLAAR
                     GVRVVRTDGTVSAELMELMQG"
     gene            12476..13600
                     /locus_tag="PKK36_05515"
     CDS             12476..13600
                     /locus_tag="PKK36_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94052.1"
                     /translation="MGNRKILLVLNDLRIGGAETQALVLAGYLKTRGWEPVILGLASP
                     GPLQDRCREKGIQCVYSAFPYPWSRWYRPFYFLKMWWKLFCLKPDVVLAYTGVPNVLC
                     GYTWRAAGVKACIWNQRSGAIARMPHHLEQGAARRMSAFIANSRSGFDFLTGTLRVPA
                     DKVSLIPNGIELIERSPDSRRAVREKLGIDAGALVAAMIGNLREPKDQETLIRAWPAV
                     MAHAAGQGRSAVLLLAGRMFDEFASLPKLAESLACDKAVRFLGYQPDVKELLSAVDVC
                     VFSSNSEGLPNGVLESMAAGLPVVATALPGIRSALPELQRWALVPPESPERFAEKIIA
                     MSDPARAAECGAANRMRIEQEYSVDRMGRSMTAVLERSLQ"
     gene            13597..14823
                     /locus_tag="PKK36_05520"
     CDS             13597..14823
                     /locus_tag="PKK36_05520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94053.1"
                     /translation="MKILHLCDKLTGGGDAVAMCRLHAGLLRAGHESRILVARNEPGV
                     GTAEIALYPPRTLMQKVHYFVGILRGINYAAIPGTLEAVTGHPFWNWADVVHINTLHG
                     GYFNYRALHELGLLKPLVWTLHDMWAITGHCSHSLDCPLWEKGCGKCPHPEIYPAIRR
                     DATMQEWKWKQEAFNTVRQFICPSEWLAEKARRSLLSPAPVHQLFIGLDLETYCPRDK
                     SESKALCGLPADLHVLLYASQSLNNPFKDAAMMPEILNALDAEIKSRCVLAVMGGGGG
                     IKTFEGCGVPVMDMGYVSDEQRKIALYSAADMLVYPTRADNLPLIIQEAMACGCPAAS
                     VAVGGIPEMVHHDETGILVQPGDARGMAGQLGRVLGSLDVHERMSKACREFAEKHFAL
                     QPYVEAVVKIYRAALK"
     gene            14820..15674
                     /locus_tag="PKK36_05525"
     CDS             14820..15674
                     /locus_tag="PKK36_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR94054.1"
                     /translation="MKISIIVPVFNAADSIGRCIESVLGQQGAEVELIVVDGGSTDGT
                     VDIIRRYRDHIAWWVSEPDTGQTSAINKGLSRSTGEVVNWLCADDVLTEGALKFVAEY
                     FAAHPDADLLAGACEMVYEENPAKNFIFIPRADFPDLLPAFDGIMQPSCFWRRRIMTR
                     TPLLDESFHYGMDAELWCRFKSQRAVMVQTDQLLSRFIQSGANKTATGGKKIGLELDA
                     IYRRYSNDKVPLSFWYRHLRYPFELYVRRDRGILRLALLRMIQAAYMAVFMPFYGVKK
                     VYYMSWPA"
     gene            15671..16768
                     /locus_tag="PKK36_05530"
     CDS             15671..16768
                     /locus_tag="PKK36_05530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94055.1"
                     /translation="MSRKKIVILTPYCPLPADTGGKMEMMKHLNVLRELGDCTILSAS
                     RKPVGAGWDEENTRKIRDMGFRVVLRETPANPLQWAGMAYASACKALRLEKAFGHSNP
                     YHRFAFPLAWWEKHTADADLVVTNYSYWSWLPATCPKACVLLDLWSNYMWEGMRREVQ
                     DLSTCGQVFVISRSEEKQLREAGVHRTFWSPPAFPGRELPVTGEVGLVGSNSLFNREG
                     LSWLASAHASHPRIRVYGKLAAAAEAPMFNRVGTYSDHDQPYRECGIILFTTVLGMGV
                     QIKTIEALAAGRAIIARRGAARGLPTDTKGWIEVDTPEEMISTAKRLQDDLSLRERQS
                     FAAREYYRLHLDSRNILSNLAAEYEKMLKNG"
BASE COUNT         3589 a         4073 c         5074 g         4103 t
ORIGIN      
        1 taatgattca ctcgctgctt atgagaggtg cagtcatgca gagttaatga atatttatgc
       61 gcgtgcagta gatcgcgcaa accgatagcg aattattgtg cggaaactga agataaattt
      121 agacagcatc agagaccggt aaaagcatgc aatccaatac agacacgcaa gttgtagccg
      181 ttattcccgc aagggggggc tcaaagggta tccccgggaa gaatatacgg ctattggctg
      241 gcaaaccttt gttggcctgg acggtggaag ccgctgtggc cgcgaacagt attgattcgg
      301 tttatgtttc gactgatgat ccggcgatcg cgaaggttgc ggaggcctgt ggtgctgaag
      361 taattttccg gccggcggat atttccgggg atgaaagccc gtcagaagcc gctttgctgc
      421 atgctttggg ctatctgaat aaacagggaa tggttgttga aataatggtg ttcctgcagg
      481 caacttctcc cctacgaacg tctgccgaca ttgattgtgc catggcactg ttccaagagc
      541 agcaggccga ttctttatta tcggttgccc caagccatta ttttttgtgg gaagaaacga
      601 aggaaggcgc tcaaccggtg aattatgact attgccatcg tcctcgtcgt caggatatga
      661 ttcaacaatt tagagaaaat ggtgctattt atatatttaa gccatccatt ctccgggaat
      721 atagcagccg tttggggggg cggatcattc tttataaaat gctggaggaa tcttcactgg
      781 atatcgacag ttattcggat tttgaacggg ctggggcgta tttaagaggt aggaagaatt
      841 aattatgatt attacaaaaa acatcttctc gtattgcatt tcttctgatg acagcatcta
      901 tgccgcgttg agcaaaatag atgcaaatgc cgaaggtttt gttttttgca ttgataatgc
      961 cggggtccta gagggcattc tgaccgatgg tgatttcagg cgctggctta tgaaggcaaa
     1021 gtatgtggat accagccgcc ctgttcgtga cattgcgcgg caggaatttg tcgcggcccc
     1081 tttgttgagt tcccgcgaaa agattcttcg gcttttcagc aagaaaatca gatttatccc
     1141 tttgcttgat gatcgtagac ggcttgtggg aatcgcgcgt cgccgtaatc tggctgaggg
     1201 gatattgatc ggggatctct taattagcga ccatgctcct gtatttgtta tagcggagat
     1261 aggaattaat cataacggca gcctgccgcg tgcccaccgc ctgattgatg ctgtccggga
     1321 gtccggcgcg aattgcgtta agtttcaaat gcggaatatg cacgccttgt acagaaatgt
     1381 agcagaggcc gatcttaccg gagaggattt ggggccgcag tatactctta acctcctgtc
     1441 ccgttttgaa ttgctgccgg aacaaatgct ggaactgttt gattacagtc atcaagcggg
     1501 tttgattccc ctctgcaccc cgtgggaatc tgaaagcttg aaaatcttgg aagcgcatga
     1561 catggcggct tataaagtgg catcggcaga tctgacaaat catccatttc tggaggaact
     1621 ggcccgtact tacaagcccc tgatcgtgtc aaccgggatg tcgactgaag ctgagattga
     1681 gcagtccgtg aaactgctga aacaactggg tgttgcttat gctctgcttc actgcaattc
     1741 aacctatccg gtgccattta aggatgtcaa cctgcggtat atgcaccgtc tcaaggaaat
     1801 gggagattgt gtggttggtt attcgggcca cgaacgcggt tattctgtgg cgcttgcggc
     1861 agtttctatg ggcgccaaga tcatagaaaa acacatcacc gaggataaaa ccctggaagg
     1921 aacagaccac aaggtcagtc tgctcccgca ggagttcaaa gctatggtgg aggggattcg
     1981 agaggttgaa ctggcgctgg gtgttggaga tgtccggcag atgagccagg gagaactgat
     2041 gaatcgttcg aatctggcaa aaagccttat catcaattgt gacctcaaag aaggtgagat
     2101 cattgaaccg aacatgatcg agataaaaag tccggggcgc ggactgcagc cgaattatcg
     2161 tgaggaactg attggatctg tgtctaaacg taatatggtc aaaggagatt ttttctatcc
     2221 gacggacata caaaaagaag aaatcaatgc acgcaagtat accttcagcc ggtcctgggg
     2281 ggtgactgtg cgatggcatg atttcaagga cattctctgt aaaaccaatc cggattttct
     2341 agagttccat ctgagtttca aggatatgga cgaggattat atgaagtatt ttgatcaggt
     2401 ctatgatttg gaccttaagg ttcatagtcc agataccttt gcaggggacc atctcctgga
     2461 cctttccaat ccaaatcccg accataggaa gcgttcaatc agggaattac agcgtgttgt
     2521 tgatttgaca cggaatctga aacagttttt caggcgggcg acacggccga tgatcatttg
     2581 cagcttgggc ggattttcca cagatggact gctgatcgca gataaagtca aagaacgcta
     2641 tgaaatcatg gaagacagcc tgactcagat tgatgcatcc ggggtcgaaa ttattgggca
     2701 gactcttccc cctttcccat ggtattttgg cggccagatg tacctgaatc tgtttgttca
     2761 cgcagacgac acagttgctt tctgtgaggc taaccaattg cgtttatgtt acgacatcag
     2821 tcattccaag ctaacctgca attacttcaa aacctctttt caggaattca cggcaaaggt
     2881 tgctccttat gttgcgcata tgcatattgc ggatgcaagt ggagtggacg gggaagggct
     2941 gcagattcat gagggggaaa tagattttcc ggccttcatt gctgaagtca aagcacaatg
     3001 tcctgacgcc tcatttatac cggaaatatg gcagggccat aagaatgacg gcgaaggttt
     3061 ctgggttgct cttgaacatt tggaagccat gaaattctga ggcgagaccg gattattatg
     3121 cagagaacca aattatgaat gaatcccgca cagttgaaaa tagaattgtc atccccattg
     3181 aaaccaaagt gcgcgaattt cagggcaaac tcctgtgcgc gttaacactg gctgaacgtg
     3241 gttaccgggt gatactgggt gatcagcgta tactcatgag cacgctggag cagtacactc
     3301 cttgtattta tgtggacaag agctttgcct ccacaaagcg gcttggctac acaaaatgca
     3361 aaacgctggg aaaccgtctg ctggcatggg atgaagaggg gctggtctat tttgatgctc
     3421 agacctatcg ggatctgcgg ctggatgcga actgtctgag catgctggac gcggcgttcg
     3481 cctggggtcc cgatcaggcg gaaatattga agggaattac ccctagcggg ctgccggtta
     3541 tagagatcgg ccatccgcgc atggatctgc tgagacctga gttccgcgga ttttataaga
     3601 caccagtgga gaaacttcag tcaaaatatg gaaagatgtt gctggtgaat acagcatttc
     3661 cgctggccaa tcattttcac ggggacatgc agatacaaca gaaacaggaa ctatatccaa
     3721 tcgcacagca ccgccctgac ttctttgaga agtggaaaaa agttcagtat gccggtatgg
     3781 tcagctttaa agaaatgatt cccgagcttt gccgagcatt cccggaatat acagttgttg
     3841 ttcgtccaca tccttctgaa aaacggggaa tatgggaatc ttttacttcc gaaatcaata
     3901 atgctgtagt taatgcagac gggaatgtgg ttgaatggat cctggcatca gaatgtgtga
     3961 tacatttcga ttgcaccagt ggactcgagg cttttctgct ggaagttcct gcggcggttt
     4021 acaggcggtc agcatctccg ggatatgaac agcagctccc gaattcttta agctacaggg
     4081 tacaaacctg tgacgaagtg ctgagctttg taaggggggt gttggcgggt cagaagtcgc
     4141 ggctactcga cgatcctgag cgtatggcgt tgctccggcg acatatggtt gcggctgatg
     4201 gggagttaag ctgcgacaga tttgcagatg cattggatgc attagcatta acgcgacttc
     4261 cccagaaagc atcagaaaag ataccctcat tgaatgttgc atggcgctgg tggttgaagc
     4321 gctatcgcag aatgaatctg aaacattcgg cttatgcaca tcagaaattt tctggactat
     4381 ctgtgcaaga gatagaacgc gtaacagaat ccatggcaga agcgctggga agattttcct
     4441 ctgttgcaat tcatcagatt gatgaagcgg tatttcaaat tacaggcttt aaagacgatg
     4501 ttttatgagt gaatatataa gaacttcata catagccgga atttccagct caaaacatcc
     4561 tgcacgtcaa ctccgtatga tcctgttccg ctggaatatt cctgctgttg tctgcaggct
     4621 tttgcgccga aaatattcaa tcatagaaat tgcactcgtt ttgtgtttgg ttgtcttggc
     4681 atgggggagc ggcagtgatt tacccgatac gcaggccgta aagtccgatg cgcgcgccta
     4741 tgcgacactg gcagttaatc tgtggcggca tggtgtgttt tcatctgacg aagcagatac
     4801 cgtaagtcca atgccgaccc aatatcgcga accggcctat ccggcatttt tggcgcttgt
     4861 gatcgccacc catcgagagc ttcggaataa acctatggag aatttcttgt cggcagggaa
     4921 agatatccgt tctctcaagc ggtcgggtct tctgcttttt gtcttactgc cggtattgat
     4981 ttatgtgctt gtccgcaaag cgggtggctc tcgtgcagcg gcgctcagtg ctggattgct
     5041 ggtaattatg gaccgtggct ctcagaatct tcttgcgacc acactcagcg aacatcttac
     5101 gatggttgca atggctgctg tttcgctttc cctgatggga ctggtgagac ggccgggaac
     5161 tggttgcgcg gcattgggtg gacttgctct tgctgtgctg gtgttaacca agacagtgtt
     5221 ccaactattt ctgcccgtgg cactgctttg ggtatggtgg aacggtcgtg gccttacggt
     5281 ctcgcggcga tggatcgcgg tcgcagtatt tgccggggcg tttgccctgc ctgtcggagc
     5341 atggaaattg cgcaatgtcg cgcatttcgg caatgcttcg ctggtgtcgg ggcgcagcgg
     5401 agtggttttg atggcccgag cgttgtcgga cactatgact cctgaggagc gtcgtgcggc
     5461 ttggatttac tggatgccgg aaggcaagct gaggaatcgt tgcgcaagat ccgctttcgg
     5521 gaatcgcttt gaagaggcta cgcggcggct cgatcgccgt tgtcctgaga gtttttatcg
     5581 gcgggcccgc atcgaacgcc aaaccttgct cgaggaaatg ggtgcagggc cgagagtgca
     5641 tcacgaaatg gatcgccgac ttacccggta cgccttaaat atcatacagc aacatgccgg
     5701 ggcacatttg gcgcttaccc ctgcctttgc ttggcgctgt ttgttttcga cccgtggtct
     5761 gcgactcctt tggccattta catggaaatg ggaggctccg ttgcggtgga ccatgctatg
     5821 ggctgggggg ctattgattg gcactgtcat gtcattcctc cgccgcgata tttgcgtgct
     5881 ggctttgaca ttgccatctc tgttccttct tggatcccat gcatttttga cgcactgtgt
     5941 tccacgctac gggttgggga tggtaccatt tggaataggt gcgctcgcgc tggtgggtga
     6001 cgccactaca cgcagattgg caaaaagata taagcggtgg aaagatcaga atccgctcag
     6061 aagggagatc cgtgaagaaa ctcatcatcc agattccttg cctgaatgag gaagaacata
     6121 ttggacatac tcttgcttcg ttgccgcgcg ctgtaccggg attcgatcag gtcgaattgc
     6181 tggtaattga tgacggcagt tcagataaca cagtggctgt cgcccgcgcg gcgggcgccg
     6241 atcatgttgt cagcctgtct tcccatcagg ggcttgcccg cgctttcatt gccggactcg
     6301 atcgggcggt tgaacttggt gccgacgtta tcgttaatac cgatgcggac aaccagtatt
     6361 gcgccgatga tattccccaa ctgactttgc cggtgctgga ggggcgcgcg gatatggttg
     6421 tcggagagag gcccatagag tctatcgggc atttttctgc cgcgaagaaa cgcctgcagc
     6481 ggctgggtag ctgggttgtc cgcgcagcca gcggaactca gattccagat gcccccagtg
     6541 gatttcgagc gctgagtgct gatgctgcgc gccgactgaa tatcttcagc aactacactt
     6601 acacgctcga aacgatcatt cagacggggc acaagaatat gagcgtgctg tccgttcctg
     6661 tccggacaaa cgcctttgtg cgcccgtcgc ggcttatgaa aagcacactt cgctatgtct
     6721 ggcactcctt gattaccata gtccgtattt ttgtggtgta tcggcctttc cgttttttca
     6781 tgaccgtggg cggtgtatta tttggaatag ggctgctgat aggcatccgc tttctctatt
     6841 atttcatgat tgggggtgga gaggggaagg ttcagtcttt gattctcgca tccatcctgc
     6901 tcgggattgg attccaaaac atgctggtgg catttatggc cgatttactg tcggtcaacc
     6961 ggaggttgat agaggatgtt caataccggc ttaggtgcgg tgaggtttat ccgcgcgcgt
     7021 cgggagagaa ggagaagtgc gatgtctgat caaggagcag agggaatact ttctgcgtgg
     7081 ttgcgccgtc atcggcttgc ggctacccgt ccgcatttgc gcgggacggt gctggatata
     7141 ggttgcgggg caggcgcgct agcggatgag tatcccgcag aacactatgt gggcgtggat
     7201 attgacgagg aatcgttggc tgtggcacga caccatcatc ctgaacatca attttgtgcc
     7261 gccgctgatc ccgagttgcg agtcaatact atagcccttt tggccgcaat tgaacatgtg
     7321 gctgatcctg caggatttct ccgcggactg gcgaatcatt tgttgcccgg cggccatttc
     7381 gtgctcacca cgcccgcgcc atggaccgat cgcgtccacg cctggggtgc ggcgatgggg
     7441 ttgtttagcc atgcggccca tgaagagcat gaaaagctga tcgatccgcc gtatatgcgc
     7501 gccatctctc attctgctgg cttgcgtgtc acctatatgc ggcgatttct ctttggcgcg
     7561 aatcaactat tcattcttga tcgcgacccg actaaggaaa ggagcacctt gtgatatctc
     7621 tgtttcggta tatgagagag gctcttatgc ggggcgtgca ggttgtggta ggtactgcgc
     7681 ttttcgatgt ttggcccctg ctgtccctgc ggcacgcaat tctgcggctg tattttcagg
     7741 cgggcgcagg cttcctggtg ggccggcatt ttcagttcca tcgtccgcat tttctcaggg
     7801 ctcctgctgg tgcgcggctc actttcgcac agaatgtgaa aatcaatagc aacgtagaaa
     7861 tcgattattc aggtggcgtc gagattgccg acgacgtttg gatcagccaa aatgttatta
     7921 tcgagaccca cgaacatgtc attggatctg gccctaaatc gggttggctg gtgaaatggt
     7981 ttccactgga aataggacgt gacgcttgga ttggagccaa tgcgatcatt ctgccgggag
     8041 tgaaacgaat tggcgcgaga gccattgttg gagcggcagc ggtagtcagc cgggaggttc
     8101 tggatggaga aatcgtcgga ggcgttccgg cgcgaatgat tggacagcgg cccgctgagc
     8161 gggagggaga atagatgggc gctgatggca gtcattcagc atggcgatgg atcgggcggt
     8221 ttctctcttt gtgcggactt cttttcgttg cgtggcgggc ttggccgctg cggacggatt
     8281 tggcacaagc tcttgcgggg ccgggcgtgc gcacggcctt cattctggcc attggagtat
     8341 tccttctcga caatgttata cttgcttcta tctggggtgg gctgttgagg gtctgcggtg
     8401 caacaaaatt cgccatgcgc actacgctgt ggctgtatgc acagacgcag attgcgaaat
     8461 atcttccggg aaatatcatg cattttgcag ggcggcatgc agcggcgcgt agccgcggtg
     8521 tcgctcatgc tccgctggcg gcggctgcga tgatggaatt tggggggctt attgcggcag
     8581 cgggaggagt ggctctcata gggttgtcat taagcgcggg agatggacag cgtactgtaa
     8641 tggttcgact agcggtaatt tctcttatcg tgttgcttgg gctatcggct ctgatttgct
     8701 tagtaccccg aacggcggtt ttccgacgac ttggtggtgg cgtccttgtt cccatcgtgg
     8761 ttgctcgtgg tctatccgtc gcgatcctcg gatatgttgt attctttttg atcggcggga
     8821 tgagttgtat ccttcttgct aatgccttgt cgggagatgg attccacggc tcagggagca
     8881 ttttgttcgc ttacgcggtt tcttggatta tggggtttat aactccagga gctcccggcg
     8941 gattgggtgt ccgcgaggcc gcgttgtcgg gattgttgac agcgcagctg ggtgctgccc
     9001 cggcagtatt ggcggcatta ctcttcaggg gtgttactgt gggcgcggat gtactgtttt
     9061 atgccggttc atttgtttgg acaccgatga atgaaaggcg aaatctgccg tgcgaggaac
     9121 gattagggct atgattaaag cagaacttat gttctggagg caaaaagaat gccccgtgga
     9181 ttggtcaaat ggttcttccg tgtctattag tggcgttaga agggattata ggcggtatga
     9241 tgcgcaagaa tatattattg cggtttaagc atttggagaa gctccagtta tgaagcatat
     9301 catcataaat atgattgaga tactagttta cttccgccag cgttatctgc gcattggtat
     9361 tattctggtg atctgcctag tgctgatgtc ggtgctggag ggtgtgggca ttggtttgtt
     9421 ccttccgctg tttgaattga tcggcaaggg ctcagaggtc ggaacaagcc ctatcagtca
     9481 gctggtcgcc cgactgtttg ctgtatttca cctgccgtta acactggggc cgattgtgct
     9541 ttttgtgatt gtgatgatgt tgaccaaggg actggtccgg tggctcacca tgcggcaggt
     9601 ggcatggatg accgcgcaga tcagtgtgga catgcgcttg gagctgttcg atgcgctgct
     9661 tcgggcggac tgggattatg cctccagtcg tgccgccggg aggttcgcca atgcggtagg
     9721 cgtggagacc aaaatatcgg cacatatgtt tcgtcacatt atcgtcacaa ttgcctgttt
     9781 tctgcaggtt ttcgtttatc tgctgctggc actggtggtc tcctggaagg tggcgctgct
     9841 gtcgatactc gccggtttgt ttatgcttct actgttccac aaactgatca tgattgccaa
     9901 gatggcaggc aagcagaccg tgctgctcca gcgggcactg gtgatccggc tgatagacat
     9961 cgtgggcggt ctcaaatcga tgaaagcaat ggggcaggag gcgctggttt atcccatctt
    10021 gaaggccaag acggagaaac tgcgcaaggc ggaggcgcag tcgatgatga ccgtagaaac
    10081 catgtccgca ttccaggagc ccctgaccat ggtcatgctg gccatcggcg cgtatgtgct
    10141 gctgaaattc acggccattg aatttgcccc tttcgtcatg ctggcgctgc tgtttacgcg
    10201 ttcggtcggg caaatcaatc agatgattac gaattatcag gccatcgcag tgaaagaggg
    10261 cggtttcttt gagctgcgga aagaagttgc cgcggcgcgg gcgcatcagg aggttggcct
    10321 gctggatgaa gtcagcgtgg acctttcccg gagtatcgcc ctgcagggtg tgacaaagaa
    10381 atatgccgac gccgaaccgg ttcttgagaa tatcagcctg gaaatccggc ggggccagat
    10441 gatcgcattc cggggtccgt cgggatgcgg gaagaccacc atggccgaca tcatctccgg
    10501 catcattcgt ccgacttcgg ggcaggtgct cgtggacggg gaaccgctga cggatgtccg
    10561 gctgaagggc tggcggcacc gtatcggata tgtcccgcag gaagtgcttc tgctcgagga
    10621 ttcagtatat gcgaatgtaa ccctgggaga ccgcaggata tcccgcgaaa agtgcattga
    10681 agcgctgaag aaagccaatg cgtgggattt tgtatcccga ctccaggata acattgatta
    10741 tgtggttggc cgcgcgggag cgggtctttc cgggggacag cgccagcgca tagccatcgc
    10801 cagggcgctg gtgcgcgatc cggccgtgct ggtgctggac gaagtcaccc gttcactgga
    10861 ccgcgagtcg gaaaaatcaa tctgcgaatc cattgcggcc ctgaagggaa gcatcaccat
    10921 tattgcgatc tcccatcagg aggcgtttat ctcactggcc gacgagctgt gggaatttga
    10981 gggaagcgga aaagtcatcg tgaaaaaaca tctgttcaaa tgaacctgct caacaaagtg
    11041 ctgattcgtc tgcttggccg tcgccttgag ctgattcagg tggcggaata tccgaagtgc
    11101 ggcgggagct gggtggcgcg gatgatccgg tcgtatgtcg gcgtggatcg aaaatacggg
    11161 attacagcgc ccgtttgccg gcatgccgtc atacagacgc atgatctgta cacctctttc
    11221 tatgcgcgca acattgttgt gatccgggat ccccgcgatg tctgggtttc gttctatttt
    11281 cacgaacgtt atctgaagcg cacggctcct gtttatgact tcgacgatgc cctgagccac
    11341 gctcagaaca tgctccggtg catgcgccag aagctgtctg cgcccgaaaa gagcatgccc
    11401 ggtttctcct atgccgattt tctgaaatcc tgggaaggcc ggcccgcgac ggcgcatgtg
    11461 aaatacgagg ccctgcatgc ggatgcgcag ggggaactgt cccgcctggt gcgcgcactg
    11521 gacatggagc ccgaatccgg ccggatcacg gccgcgattg aagccaaccg gtttgagaaa
    11581 atagcaggcc gtgaaccggg gcaggaggat ttcaccaagc acaagcgcaa gggcatcgtc
    11641 ggcgactgga aaaattattt cacaagggaa atgggcgacc tggtgcacca gcaccagcct
    11701 tttctttacg aactgggcta tgagccggac gactcgtggc tgaaggagct cccgcaatga
    11761 gtcctgaatt catcgaggtg tttggcattc cggcttccgg gaaaaccatg cttgtcaatc
    11821 agctgtgcgg cacacatggc gccgtaagcc gcgaggaagc gctgcggggc gcgctcacgg
    11881 tgaaacgcct gggcctcgtc cggtggatgc tgcacaggtg cggactttcc ggcggatcgt
    11941 ttgatgagat cacgtgcggc cttctggagc agagtgccct tgcgaatcgc cggctggtcc
    12001 agaccgtgtt cgactatctg gtgcaggccg gtgttcccga ccgcgaggcg ggtgtggctc
    12061 tgcgggggat attccgcacg gccagagagt acgcgctgct gaccgaggct tcagcagatg
    12121 cgcgtatcct gatggacgag ggctggattc atcgggcgct cacggtcttt ggcatgcggg
    12181 cctcggatga agtccccgac gaatggctgc agcgctatgc ggactcggtt cccgcgcccc
    12241 gtttcgcgat ttatgtcgaa tgcgatcccg aaacatcctt ttcacgtgta tccgcccgcg
    12301 agagaaaagc ccgattctgg tggggcgaat ggacggaggc ggagcagagg agggcgcttg
    12361 cgcagactgc ccggtgtgcc gcacgaatcg cggaacatct ggccgcccgg ggagtgcgcg
    12421 tcgtccggac agatggaact gtttccgctg aactgatgga gttgatgcag ggctgatggg
    12481 aaaccggaaa atacttctgg tgctgaacga tctgcgcatt ggaggcgcgg agacgcaggc
    12541 gcttgtgctt gccggttatc ttaagactcg tggatgggag ccggtgatcc tggggctggc
    12601 cagccccggc ccgctgcagg accgatgccg tgaaaagggg attcaatgcg tctacagcgc
    12661 attcccgtat ccatggagcc gctggtatcg cccgttttat tttctgaaga tgtggtggaa
    12721 actgttctgt ctgaagcccg acgttgtttt ggcgtatacc ggtgtgccga atgttctttg
    12781 cggctatacc tggcgggccg ccggggtgaa agcctgcatc tggaaccagc ggtccggggc
    12841 cattgcccgc atgccgcacc atctggaaca gggcgcggcg cgcaggatgt cggcgttcat
    12901 cgccaattcc cggtctggct ttgatttcct gaccgggaca ctgcgggtgc cggctgataa
    12961 agtatcgctg attccgaatg gcattgagtt gatcgaacgc tctccggatt cacgcagggc
    13021 ggtcagggag aagcttggga ttgatgccgg ggcgctggtg gccgccatga ttggaaatct
    13081 gcgtgaaccc aaggatcagg aaacgctgat tcgcgcgtgg ccggctgtaa tggcgcacgc
    13141 ggccgggcag gggcgttcag cggtgctgct tctcgccggc cggatgtttg atgagttcgc
    13201 atcgcttcca aaacttgcag aatctctggc gtgtgataaa gctgtgcggt ttctcggata
    13261 tcagcccgat gtgaaagagc ttctgtcggc ggtggatgta tgtgttttca gttccaacag
    13321 tgaagggctt cctaatggcg tgctggaaag catggcggca ggccttccgg ttgtggccac
    13381 tgctctgccc ggcattcgca gcgcactgcc ggaactgcag cgctgggctc tcgtcccgcc
    13441 tgaatcgccg gaacggtttg ccgaaaaaat cattgccatg agcgaccccg cccgcgcggc
    13501 ggagtgcgga gcggccaacc gcatgcgcat tgaacaggaa tattccgttg atcgcatggg
    13561 ccggagcatg acggccgtac tggaaaggag tctccagtga agattcttca tctgtgcgac
    13621 aagctgacgg gcggcggtga tgccgtggcc atgtgccgcc tgcatgccgg gcttcttcgg
    13681 gccggacacg aatcgcgcat tctggtggcc cgaaacgaac ccggtgtggg caccgcggaa
    13741 atcgcgctgt atccgccgcg aaccctgatg cagaaagtgc attattttgt cgggattctc
    13801 cgggggatca attatgcggc gatccccggc acgctggagg ccgtgacggg gcatccgttc
    13861 tggaactggg ctgatgtggt tcacatcaac accctgcatg gcggctattt caactatcgt
    13921 gcgctgcatg aactgggcct gctgaagccg ctggtgtgga ctctgcacga catgtgggcg
    13981 atcacgggtc actgctcgca tagtctggac tgcccgctgt gggagaaggg ctgcgggaaa
    14041 tgtccgcatc ccgaaatcta tccggccatc cgccgcgacg ccaccatgca ggaatggaag
    14101 tggaagcagg aggcattcaa tacagttcgg cagttcatct gccctagcga atggctggcc
    14161 gaaaaggccc gtcgcagcct gctcagtccc gctccggttc atcagctgtt cattggtctc
    14221 gatttggaaa catattgccc gcgtgacaag agcgaatcca aagccctgtg cggactgccg
    14281 gcggaccttc acgtgctgct gtatgcctca cagagcctta ataatccgtt caaggatgcc
    14341 gccatgatgc cggagatttt aaacgcgctg gacgcggaga tcaaatcccg ctgtgtgctg
    14401 gccgtgatgg gcggcggcgg ggggatcaag acgtttgaag gctgcggagt gccggtaatg
    14461 gatatgggtt atgtgtccga cgagcagcgc aaaattgccc tctacagcgc ggccgacatg
    14521 ctggtttatc cgaccagggc ggacaacctg ccgctcatca ttcaggaggc gatggcctgc
    14581 ggctgtccgg cggcctccgt cgcggtcggc ggcattcccg aaatggttca tcacgatgaa
    14641 accgggattc tggtgcagcc gggcgatgcc cggggaatgg ccggacagct aggccgggtg
    14701 ctcgggagcc ttgacgttca cgagcggatg agcaaagcct gccgggagtt cgcagaaaaa
    14761 cattttgcgc ttcagccgta tgttgaagcg gtggtgaaaa tataccgggc ggcattgaaa
    14821 tgaagatttc aatcatagtt cctgttttca acgccgcgga cagcatcggg cgctgcatcg
    14881 aaagcgtgct gggccagcag ggggctgaag tggaactgat cgtggtggac ggaggatcca
    14941 cggacggcac ggtggacatc attcgccgtt accgggatca tattgcctgg tgggtgagcg
    15001 aacccgacac aggacagacc agcgccatca acaagggcct ctcgcgcagc acgggagagg
    15061 tggtcaactg gctctgcgcg gacgacgtgc tgacagaagg cgctttgaag ttcgtggcgg
    15121 aatattttgc ggcccatccc gatgctgatc tgctggccgg cgcctgcgaa atggtttatg
    15181 aggaaaaccc cgcgaaaaat tttattttta ttccgcgcgc ggatttcccg gacctgctgc
    15241 cggcctttga cggcatcatg cagccgagct gtttctggcg gcgcagaata atgacgcgca
    15301 cgccgcttct ggacgagtcc tttcactacg gcatggatgc ggagctgtgg tgccggttca
    15361 agtcgcagcg tgcggtcatg gttcagacgg atcagctcct gagccggttc atccagagcg
    15421 gagccaacaa aacggccacc ggagggaaga agattggcct tgagctggat gcgatctacc
    15481 ggcgctattc gaatgacaag gttcccttgt ccttctggta ccggcatttg cgttatccgt
    15541 ttgagcttta tgtccggcgc gaccggggaa tcctgcgcct ggcgcttttg cgaatgatcc
    15601 aggcggcgta catggcggtt ttcatgccgt tctacggggt taagaaggtt tattatatga
    15661 gctggcccgc atgagcagaa agaagatagt tattctgact ccgtactgtc cgctcccggc
    15721 cgacacgggc ggaaagatgg aaatgatgaa gcatctcaat gtgcttcgcg aactgggcga
    15781 ctgcaccatt ctttccgcaa gccgcaaacc ggtcggggca gggtgggatg aggaaaacac
    15841 ccggaagatc cgcgatatgg ggttccgggt ggttcttcgc gagacgcccg cgaatccgct
    15901 tcagtgggcg ggcatggcct atgcctccgc ctgcaaggcg ctgcgccttg agaaggcgtt
    15961 tggacattcc aatccctatc accggtttgc attcccgctt gcatggtggg aaaagcatac
    16021 cgcggatgcc gacctcgtcg tgaccaacta cagctactgg tcatggcttc ctgcgacctg
    16081 tccaaaggcc tgcgttctgc tggacctctg gtcgaattat atgtgggagg gcatgcggcg
    16141 cgaagtgcag gatttgagca cctgcggcca ggtgttcgtc atctcccgtt cggaagaaaa
    16201 gcagctccgc gaggcgggcg tgcatcggac tttctggagt cctccggcgt ttcccgggcg
    16261 ggagctgcct gtgactggtg aagtcggact ggtgggttca aacagtcttt tcaaccgcga
    16321 agggctgtca tggctggcat cggcgcatgc ctctcatccg cgcatacgcg tgtacggcaa
    16381 actggcggcc gcggccgagg ctccaatgtt caaccgggtg ggaacctatt ccgatcatga
    16441 ccagccgtac cgggaatgcg ggatcattct tttcacgacg gttctcggca tgggcgtgca
    16501 gattaaaacc atcgaggcgc tggcagcggg cagggcgatc attgcccgcc gcggagccgc
    16561 gcgcggcctg ccgacggata caaagggctg gattgaagtg gacactccgg aagaaatgat
    16621 cagcacggca aagaggctgc aagacgactt atcgctccgg gaacggcagt ctttcgcagc
    16681 gcgtgaatat taccggctcc atctcgattc caggaatatt ttgtcaaatc ttgcggccga
    16741 atatgagaaa atgctgaaaa atggctgaaa aagccgaaaa ataacggttc ttctacgatt
    16801 ttcatggaaa tattggccca aacgcgagat agactgggg
//
LOCUS       DAOQWR010000048        16730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_3866_length_16730_cov_5.343388, whole genome shotgun sequence.
ACCESSION   DAOQWR010000048 DAOQWR010000000
VERSION     DAOQWR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16730
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_16730_cov_5.343388"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKK36_05535"
     CDS             complement(<1..858)
                     /locus_tag="PKK36_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNR94056.1"
                     /translation="MKNKLIGKLNDRSALIGIVGLGYVGLPLMLRFIESGYRVLGLDI
                     DPKKVATLNRGGSYIEHIPAAKIKNARKHGFEATTDFSLSRKADALIICVPTPLDEHR
                     EPDLSYVLNTQDSLTPFMRRGQVVSLESTTYPGTTDEELRPRIESAGLVVGKDAFLVF
                     SPEREDPGNPNFQTRTIPKVVGGTTPACLEVGLALYGQVIDRLVPVSSTRVAEMTKLL
                     ENIHRAVNIGLVNEMKIVADKMGVDIFEVIDAAATKPFGFVPYRPGPGLGGHCIPIDP
                     FYLTWKAREY"
     gene            complement(872..2011)
                     /locus_tag="PKK36_05540"
     CDS             complement(872..2011)
                     /locus_tag="PKK36_05540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR94057.1"
                     /translation="MNVPLLDLKAQYKTIRDEIRPVVDEIFESQYFILGPQVQKCEEL
                     VAAYCQCPHAVGVSSGSDALIIALMAEDIGPGDEVITTPYTFFATVGAIARVGAAPVF
                     VDIDPVTFNMDMKQVAEAVTPATKAVIPVHLYGQMADMDPLMAIAAKHRLIVIEDAAQ
                     AIGAEYKGRRAGSIGDYGCFSFFPSKNLGGAGDGGIVTMRDKAKADRVRMLRNHGMEP
                     KYYHKFVGGNFRLDALQAGVINVKLPHLDSWSEGRQANARTYDRLFSEAGLVPGKVRL
                     PAHPHADPKKKSDHRHIFNQYVIRVEDRDALRAHLTGRGVGCEIYYPVPLHLQDCFAC
                     LGHQKGDFPFSEKAADETLALPIYPELTDEQQACVVNCIKEFYGA"
     gene            complement(2080..3000)
                     /locus_tag="PKK36_05545"
     CDS             complement(2080..3000)
                     /locus_tag="PKK36_05545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNR94058.1"
                     /translation="MHKNPRILILRGGAIGDFIVTLPALQALRRRWPEAFIELLGYPH
                     VARLALGGGLVNTVRSLDEAGMARFFAPMASFDPEQVNFVRSFDLIVSWLHDPDEVVR
                     NNFLYAGARQVISGTPLVQKFHACECMLKPLEELAIYSEHEVPRLDVLRELKAAGCEW
                     LRRHGLETAVTAIHPGSGSPRKNWAPGNFIQLARALRERGKDCFFILGEADAECGEAV
                     RRNAPDFPVLDGCGLEELAGTLAHAALYLGNDSGITHLAAALGMRVIALYGPTDPALW
                     GARGREVVHIQAPGGSLNRLEVGEVLNFIE"
     gene            complement(3382..3732)
                     /locus_tag="PKK36_05550"
     CDS             complement(3382..3732)
                     /locus_tag="PKK36_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNR94059.1"
                     /translation="MELKGEAKLLRIFIGESDKVAHVPLYEAIVKAAREGGLAGATAW
                     RGMLAYGPGSRIKTAKILDLSGDMPVIIEITDEEAKINAFLPKLHDLIETANSGGLVT
                     VERVQIIKYLHAKK"
     gene            complement(3739..4119)
                     /gene="crcB"
                     /locus_tag="PKK36_05555"
     CDS             complement(3739..4119)
                     /gene="crcB"
                     /locus_tag="PKK36_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNR94060.1"
                     /translation="MVPILAVGFGGFIGAVLRYLLSLTGQRFSITFPHGTLWANFLGC
                     LLLGMVTAIAVDTESLSPAARLFLATGICGGFTTMSTFTYEMFRFLQDAQYFYAAAYF
                     SATIAGCGLLFGTGLIITKLMLKA"
     gene            complement(4136..5551)
                     /gene="ahcY"
                     /locus_tag="PKK36_05560"
     CDS             complement(4136..5551)
                     /gene="ahcY"
                     /locus_tag="PKK36_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNR94061.1"
                     /translation="MNMANKKGRDFKVADIRLADFGRREMKLAEVEMPGLMALREKYG
                     KTRPLSGARITGSLHMTIQTAMLIETLQVLGAKVRWASCNIFSTQDHAAAAVAAKGTP
                     VYAYKGESLEEYWDFTDRILDWGNGRGPTTILDDGGDATLFVHLGYQAENDPSVLDRK
                     PGSHEEKVLLAQVKKAMRRDRGRFHRIVSKIIGVTEETTTGVHRLYKWAKNGQLLFPA
                     FNVNDSVTKSKFDNLYGCRHSLVDGIMRATDVMLSGKIAVVAGYGDVGKGSCQSLKGQ
                     GARVVVTEIDPICALQATMEGYEVMTMDEACLIGDIFVTTTGCCDVITEKHMLKMKNQ
                     AIVCNIGHFDSEIQVERMRKYKWENIKPQVDKITMPSGRSILLLAEGRLVNLGCATGH
                     PSFVMSNSFTNQVLAQMELYAKQGDLPIGVYTLPKILDEEVARLHLGKLGVKLETLKR
                     HQASYLGVPVTGPFKPDHYRY"
     gene            complement(5608..6744)
                     /gene="ftsW"
                     /locus_tag="PKK36_05565"
     CDS             complement(5608..6744)
                     /gene="ftsW"
                     /locus_tag="PKK36_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNR94062.1"
                     /translation="MRNTASLVTGAVLILLTLGIIMLYSASAMAGQEQYQDPQYFFRR
                     QLVWLILSLCAGALLSQINYRIWRRLAVPLALVTMALLALTLIPGIGTTVKGSSRWLR
                     LGPLSLQPSEFAKISMILLMAWWMSRIQRRAREFVRGMLIPSVFLGAMLLLVFLEPDF
                     GTTMLIALVGASMMFVVGVRLSYLLIAGSLGLTAFFFAVMHNAERWRRIIAFLNPEKY
                     EASEAWQLLNARYAFVMGGATGAGLGQSVQKRFYLPEAHTDFIFAIIGEELGLAVSLM
                     VLVLFIMIFIGGLRISVCAPESFGKLTAFGITMMITAQALLNIGVVTGSLPTKGLPLP
                     FISFGGSSLLVSLSMTGILLNIARHSIAPGQEDEKLFRNRKDWA"
     gene            complement(6756..7676)
                     /locus_tag="PKK36_05570"
     CDS             complement(6756..7676)
                     /locus_tag="PKK36_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNR94063.1"
                     /translation="MKTPLLIAVITIVHVIAIGSVFFMQGCGTPPAARVEPPPAPVMP
                     PRDMPDDRVVIKAPQPVFPAPVAAPRAAAPADDDANVYVVKKGDMLSKIAVRHGVSAR
                     EIAELNNLKNMNDIRAGQKLLLPAYARSEKAAAKPAPKPAAGTLSGDTYVVAAGDSLS
                     KIAARFGVKTADLAAANQISNPNSIRIGQKLVIPKGGTASSAAPKPAPVTVAPIEEVP
                     EPQPEIPVEEEIPAPAPVKVSEPVASSAAPAGDLFTHTVATGDTLESVARLFAVLKKD
                     LAAANGLSEDAVLSAGQKLVIPFSSSAMAP"
     gene            complement(7726..9048)
                     /gene="murD"
                     /locus_tag="PKK36_05575"
     CDS             complement(7726..9048)
                     /gene="murD"
                     /locus_tag="PKK36_05575"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNR94064.1"
                     /translation="MTKKIKHALVLGLGASGRGAVRLLLAEGVPVTAVDSQPVLDAAR
                     FLEKDGAHVLAGCGALPGGSFDLAVVSPGIAEDHSWVTSLKERGIPCISELELGWSRC
                     RGRVWAITGSNGKSTLVKCCADSLTRAGRRARISGNYGLSFCEQVAEDPSADDWVLEV
                     SSFQLETVCRFRPDIGILLNVLPNHLDRHGTMQHYLELKLRMFARMRPKDVCIVPAPL
                     AWACGRSAVTFGPEEAAQFRWLDGRVVHDGKTVADYTGTLFDNDVMGPSLAALSAAFA
                     GAAVDLRFAEEAVRLFEPLPHRMQPAGTARGVEFVNNSKATNIAALAASLKMTKKPVR
                     LIAGGQAKEERFEEIKDLLAKRVQSAYLIGRDSEKMSSAWSGAVPCRLCGNLEQAIRA
                     AWADARPGEVVLLAPACTSYDQFRDFAERGEQFMRLAAAMVKETENEK"
     gene            complement(9045..10184)
                     /gene="mraY"
                     /locus_tag="PKK36_05580"
     CDS             complement(9045..10184)
                     /gene="mraY"
                     /locus_tag="PKK36_05580"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNR94065.1"
                     /translation="MFYYLYQLSERFSFLRIFQYITFRALAAAGTAFMISLIAGPWLI
                     RALRRFKIGQQIRTEDSETLLKIHGKKAGTPTMGGLLIILAAVVSTLLWAVPSNLYVQ
                     LTLATLCFMGGVGFWDDYLKLRRRQSKGLDARGKMLLQTGWVVLMAAVLLSHPATADR
                     TRNLMVPFLKNPLITNMGLLGSLIFIWLIIAGSTNAVNLTDGLDGLAAGCTGSVALAY
                     LFMAYVAGHYEFATYLQVPFVKSGGELAVFCGALLGASLGFLWLNCHPAKVFMGDTGS
                     LALGGGIAMVAILIKQELTLIIVGGVFVAEALSVMLQVAYFKFTGGRRIFLCAPLHHH
                     YEFLEKKRAEEENRDLEVVETMITTRFWIVSIICALIGVATLKIR"
     gene            complement(10187..11575)
                     /gene="murF"
                     /locus_tag="PKK36_05585"
     CDS             complement(10187..11575)
                     /gene="murF"
                     /locus_tag="PKK36_05585"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNR94066.1"
                     /translation="MKAHAFHPDELAEWSGGSWSSRPSAAITGFCQDSRQLKPGEIYV
                     AIRGERHDGHAFINEALAQGAAGAIADRDHFQSGQAAGGPLLGVENTRLALLDIAAAW
                     RSRLPGSVGGVTGSAGKTTVKELTADILSSIGPTARTRGNWNNDIGLPLSLLAMTREA
                     RYGVFEAGINHIGEMKQLCAVLQPQWAIITTVGIAHLEYFGTVERIAEEKAVLAASVP
                     GSGFVVLSADEPWYEFFRSRIRARVVSVSLAPGADADYTASRRGETVRFADRKGEQGG
                     ECEMPLPGEYFIRNALLSAAAAAQLGADLNTAAEQIGRFKPLGLRGNRICAAGVTIIN
                     DAYNANPLSMSAALQSLSGEACSGRKWAVLGDMGELGPTGPGEHRRIGEYLAKSGIER
                     LVTVGPLAEYIGRGAVQSGFARENQVSCAGLEEAAIALRDAQAGDIVLLKASRTVRLE
                     NLIELFNKDRSL"
     gene            complement(11572..13065)
                     /locus_tag="PKK36_05590"
     CDS             complement(11572..13065)
                     /locus_tag="PKK36_05590"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNR94067.1"
                     /translation="MSLQTEHTVLLDDLLKGASVLGRSGPEHSEIHGITCDSRLVRPG
                     WLYVAVRGHSQDGNQYVEEAVRRGASAVVSEARPMVPRTVAAVHVASARLALARAALI
                     FFGRPADSLRMIGVTGTNGKTTVAFMCSAILQAAGEHPGLIGTVRYEIGERVIPASRT
                     TPDAAEIQQMLAEMVRKGCRSAVMEVSSHALDQDRVFGLEFDAAVFTNLSQDHLDYHG
                     DFENYFRAKKKLFDALPEGRKRGVAVVNTSCPWGLRLSGEIGRRGPMITFGREDADVR
                     AERILSDRAGSAFHLISPWGHRDMRIRLSGGFNVGNALAAVAACGALGIDLDIMQKAL
                     ENFESAPGRLERIESSRDFSVIVDYAHTPAALENVLATLRELTGGRLITVFGCGGNRD
                     RAKRPLMGAAVTASADHALITSDNPRMEDPLSIIREIEAGCAKGRYEVEPDRRKAIEK
                     ALAMAQPGDIVLIAGKGHETFQEVGKTMLPFDDRQVAREILGEERAA"
     gene            complement(13062..14798)
                     /locus_tag="PKK36_05595"
     CDS             complement(13062..14798)
                     /locus_tag="PKK36_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR94068.1"
                     /translation="MTKQPFSWRLACVLVLILAAFGGLSAKLFWLHLNPNDALRLKIQ
                     KTLKFEHKISVGRGSIMDRNGAVMAADIAVRDVKVDPGKLNAGGHVQYVAEQLARMLD
                     LDLALLLNRLQGRTGNYAPIKRFVPADKADEIRALNLPCVHFDEGSLRNYPGGAMMAH
                     VVGFSNLNGEGSGGVEQRYNSWLRGVEGYRISEKDAHKREIFSRRSLDIPPQEGSDLY
                     LTLDSRVQYYVEKALHEAMEEHHAAGAWAIVQRVRTGEILAMASLPDFDLNHFGKVDP
                     EVHRNRAISFNYEPGSTFKVAVIAAALNEGIITQDEVFDCENGTWHYGGRPLRDYHPY
                     GLLSVQDIIKKSSNIGAAKIALKLGEGKLEKYLRDFGVGRFTGIDLPGEEGGLLYSRA
                     RWSKISVTRIAMGHEVAVTAMQMLSLISAIANDGFLMRPYVVGTARDCNGRIIYRAEP
                     EVVARPISQETAAQMRRLMARVTEQGGTGSRAALAEYTVAGKTGSAQKPVPGGYSDSA
                     HMASFVGFIPAENPEISIIVVVDEPQPLHTGGVVAAPVFKKIAEPVMRYLASRPAEWH
                     VVGGASVMEASP"
     gene            complement(14791..15198)
                     /locus_tag="PKK36_05600"
     CDS             complement(14791..15198)
                     /locus_tag="PKK36_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94069.1"
                     /translation="MSRKRKRKVHSRNTGAKNTGFPFTLAGTLLFVGSAALFYLWLGG
                     RCEALGTEIKNLEKHYSEVQDRRRVEEIKWAAMTTLPGVEQALIRHGLEMRFPDKRHI
                     VEVPASLYAGGLDESSLALHDDAHGRSRVAMND"
     gene            complement(15176..16096)
                     /gene="rsmH"
                     /locus_tag="PKK36_05605"
     CDS             complement(15176..16096)
                     /gene="rsmH"
                     /locus_tag="PKK36_05605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNR94070.1"
                     /translation="MMLEEAVAALAIRPGGIYLDGTAGSGGHTAAILERLAGTGCVVA
                     MDRDDEAIRRCRKRLAEWSNVVLVNRNYSEMGAALRDLKISGVDGILLDLGVSSDQLA
                     EPQRGFSFQQEGPLDMRMDRNSGLTAAELVNSLPEAELAALIWKWGEERQSRRIAAAI
                     VAARPVKSTRQLAEIVERAKGGRRGRIHPATQTFQALRIAVNNEFEHLEQALEDGLGL
                     LNPHGRMAVISFHSLEDRRVKRCFRAHAGRMESLQAGGADWIGERPRVALVGRKFQTA
                     GPEEMKMNPRARSARLRVVERTEDEQKKKA"
     gene            complement(16150..16368)
                     /locus_tag="PKK36_05610"
     CDS             complement(16150..16368)
                     /locus_tag="PKK36_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94071.1"
                     /translation="MGNMGSSLLLAGSLDGGRCPRRQFSIPEAGRAVASRLGLAPAVV
                     SAGMKDWEAVPKEIHTARIGRTLANYLF"
     gene            complement(16408..16680)
                     /locus_tag="PKK36_05615"
     CDS             complement(16408..16680)
                     /locus_tag="PKK36_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94072.1"
                     /translation="MESLRNLSIADERGRQLARTLASRSDWCPWDAQGRIRIKDDLLD
                     YASLTNQVVLVGTFDGFELWDPDRWSKVDTSANPDVLGDAAKYVGF"
BASE COUNT         3375 a         5351 c         4663 g         3341 t
ORIGIN      
        1 gtactcacgg gccttccatg tcagatagaa ggggtcaatc ggaatgcagt gcccgccgag
       61 gcccggcccc ggccgataag ggacaaaacc gaagggtttg gtggcggcgg catctatgac
      121 ttcgaaaatg tccacgccca tcttgtccgc gacgattttc atttcgttca cgaggccgat
      181 gttcacggcg cgatggatgt tttccagcag cttggtcatt tccgccacgc gcgtggagct
      241 gaccggcacc agccggtcaa tcacctgccc atagagggca agacccacct caaggcaggc
      301 cggggtggtt cctccgacca ccttgggaat ggtgcgggtc tggaaattgg gattgccggg
      361 gtcttcacgc tcgggggaaa acaccaggaa agcgtctttg ccgacaacaa ggcccgcgga
      421 ttcgatgcga ggtctgagtt cttcatctgt ggttcccggg taggtcgtgc tctccagcga
      481 cacgacctgc ccccggcgca tgaatggggt cagcgagtcc tgcgtgttca ggacgtagct
      541 caaatccggc tcgcggtgct cgtccagcgg cgtcggcacg cagatgatca gcgcatccgc
      601 cttgcggctg agcgagaaat cggtcgtggc ctcaaatccg tgcttccgcg catttttgat
      661 cttcgcggcg gggatatgct caatatagct tccgccccgg ttgagggtgg cgaccttttt
      721 cgggtcaatg tccagtccca gcacccggta gccgctttcg atgaagcgaa gcatcagggg
      781 cagcccgaca tagcccaggc ccacgattcc aatcagcgcc gacctgtcgt tcagttttcc
      841 gatgagttta tttttcatcc agtccgtccg ttcatgctcc gtagaattcc ttgatgcagt
      901 tcacgacaca ggcctgctgt tcatccgtaa gttccggata aatgggcagc gccagggttt
      961 catccgccgc tttctcgctg aaggggaagt cgcctttctg gtggccgagg caggcgaagc
     1021 agtcctgcag atgcaggggc acggggtagt agatttcgca ccccaccccc ctgccggtca
     1081 gatgcgcgcg cagggcgtcc cggtcttcaa cacgaatcac gtactggttg aagatgtgac
     1141 ggtgatctga ttttttcttc ggatcggcgt gcggatgggc aggcagccgc actttgcccg
     1201 gaaccagacc cgcttcactg aaaagccggt cgtatgtgcg ggcattggcc tgacggcctt
     1261 ccgaccatga atccaggtga ggcagcttca cattgatgac tcccgcctga agggcatcca
     1321 gccggaaatt gccgcccaca aacttatgat aatacttcgg ctccatgccg tggttgcgaa
     1381 gcatgcgaac ccggtcggct ttcgccttgt cgcgcatggt gacgatgccg ccatctcccg
     1441 ccccgccgag attcttggaa gggaagaagg agaaacagcc gtaatcgccg atggagcccg
     1501 cgcggcggcc cttgtattcc gcgccgatgg cctgggccgc gtcttcgatg acaatcagcc
     1561 tgtgcttcgc ggcaatggcc atcagcgggt ccatgtccgc catctggccg taaagatgaa
     1621 ccgggatgac ggctttggtg gcgggagtga cggcttccgc gacctgcttc atgtccatgt
     1681 tgaatgtaac gggatcgata tccacaaaca ccggcgcggc tcccacgcgc gcaatcgcgc
     1741 ccacggtggc gaagaaggta taaggcgtcg tgatcacttc atcaccgggc ccaatgtctt
     1801 cggccatgag ggcaatgatc agggcatcgg atccggagga aacgcctaca gcgtggggac
     1861 actggcaata ggccgcgacc agctcttcgc atttctggac ctgcgggccg agaatgaaat
     1921 actgcgactc gaaaatctca tccaccacgg ggcggatttc atctctgatc gtcttatact
     1981 gggctttcag atctagcaag ggaacattca tcagcgcctc atttcttaac cgttcgttca
     2041 agccgcatat catgcacaca aacggttcaa atgtccatat cactcaatga aattcaagac
     2101 ctcgccgacc tccagccgat tcaagctgcc gccgggggcc tgaatatgaa cgacctcccg
     2161 gccccgtgcg ccccagagcg ccggatcagt cgggccatag agggcgatca cccgcatgcc
     2221 cagcgcggcc gcaagatgag tgatccctga atcattgccg agatagaggg ccgcatgcgc
     2281 aagggttccg gccagttcct ccaggccgca gccatccagc accgggaaat ccggcgcatt
     2341 gcggcgaacg gcctccccgc attccgcgtc ggcttcgccc aggatgaaga aacagtcctt
     2401 cccccgctcg cgcagggcgc gcgccagctg aatgaaattc ccgggcgccc agttcttgcg
     2461 agggctgccg cttcccggat ggatcgccgt cacggccgtt tccaggccgt gccgccggag
     2521 ccattcgcat ccggcagcct taagttcccg aaggacatca agccgcggaa cttcgtgctc
     2581 gctgtaaatc gccagttcct cgagcggctt cagcatgcat tcgcacgcat ggaatttctg
     2641 caccagcggc gtgccgctga tcacctgccg cgcgccggca tacagaaaat tgttccgcac
     2701 cacttcatcg ggatcatgaa gccaggaaac aatgagatca aacgaccgca cgaagttcac
     2761 ctgttcagga tcaaacgatg ccatgggggc gaaaaatcgc gccatgccgg cctcgtcgag
     2821 cgaccgcacg gtattcacca gacctccgcc aagcgcaagg cgcgccacat ggggatagcc
     2881 cagcagttcg atgaaggcct ccggccagcg cctgcgcagc gcctgaagcg cgggcagcgt
     2941 cacaatgaaa tcgccgatgg cgccgcccct caaaatcaga atgcgcggat ttttatgcat
     3001 cagccttcaa aacatgaaaa tcagcgaaaa atcaagcgtt ttggggcgtt tttgagcttt
     3061 ttttgccgat tttaggcact tttcggccct tttttgcgtt ttttcggccc tttttcggct
     3121 gtctttgccg cgccaaaagc gagccgcgat caggtccggt gcggctggcc gcaaccgaaa
     3181 acggaaccgc caagggacgc gcatgaacac aaatgatgaa actgaaactt ctttgcggaa
     3241 acctgttaaa tagtactatt ttagtactat ttcgcatttt tcacgttttt tgggcgtttt
     3301 ttaacgattt ttggcgattt ttggcgactt ggggccgttt ttcgggcttt ttcagctctt
     3361 tttcggcatt ttcagcccga cctatttttt tgcgtgcagg tattttatga tctggacgcg
     3421 ttcaacggtg accagtccgc cggagttggc ggtttcaatc aggtcgtgca gtttgggcag
     3481 gaaggcattg atcttcgcct cttcatcggt gatttcgatg atgaccggca tgtcgccgga
     3541 gaggtccaga atcttggcag ttttgatgcg gctgcccggg ccataggcca gcatgccgcg
     3601 ccacgccgtg gccccggcca ggcccccttc ccgggccgct ttgacgatgg cctcatacag
     3661 cggcacatgc gcgaccttgt ctgattcgcc gataaaaatc cggaggagtt tcgcttcgcc
     3721 tttgagttcc ataaacacct acgctttcag catgagcttt gtgatgatca ggcctgttcc
     3781 aaaaaggagc ccgcatcccg cgatggttgc cgaaaaatac gccgcggcat agaaatactg
     3841 ggcatcctgg aggaagcgga acatttcata cgtgaaggtg gacattgtgg tgaatccgcc
     3901 gcagattccc gtcgccagaa aaagccgcgc tgccggcgag aggctttcgg tgtccaccgc
     3961 aatggccgtg accattccca gcagcaggca tcccaggaag ttggcccaga gcgtgccgtg
     4021 cgggaaggtg attgagaacc gctgcccggt caggctcagc agataacgga gcaccgcgcc
     4081 gatgaagccg ccgaacccga ccgccagaat gggaaccatg cccctcatcg taattttaat
     4141 agcgatagtg atccggcttg aacggtccgg tgaccggcac gccgagatac gacgcctgat
     4201 gccgcttgag cgtttccagc ttgaccccca gtttgcccag atgcagccgg gccacttcct
     4261 cgtcgagaat cttcgggagg gtgtacaccc cgattggcag atcgccctgc ttcgcataca
     4321 gctccatctg cgcaagaacc tgattggtga agctgttgga catcacaaag ctgggatgac
     4381 cggtcgcaca ccccagattc accagccggc cttccgccag caggagaatg gatcggccgc
     4441 tcggcatggt gattttgtcc acctgcggct tgatgttctc ccatttgtat ttgcgcatgc
     4501 gctccacctg gatctcgctg tcgaaatgac caatgttgca cacaatcgcc tgattcttca
     4561 ttttcagcat gtgtttttca gtgatcacgt cgcagcaccc ggtggtggtg acaaagatgt
     4621 cgccgatcag acaggcttcg tccatggtca tgacctcata accttccatg gtggcctgca
     4681 gcgcgcagat ggggtcgatc tccgtgacga ccacgcgggc gccctgcccc ttcaacgact
     4741 ggcaggagcc cttgccgacg tcgccataac cggcgaccac ggcgatcttg ccggacagca
     4801 tgacatccgt ggcgcgcata atgccgtcca ccagcgagtg acggcagccg tagagattgt
     4861 cgaatttgga ttttgtgacg gagtcattga cgttaaaggc cgggaacagc agctggccgt
     4921 ttttggccca cttgtagaga cggtgcactc cggtcgtggt ttcctctgtc acgccgatga
     4981 tctttgaaac gatgcggtgg aagcggcccc tgtcgcggcg catggccttt ttcacctgcg
     5041 ccagcagcac tttttcttca tggcttccgg gcttgcggtc aaggaccgag ggatcatttt
     5101 ccgcctgata tccgagatgg acgaagagcg tggcgtcgcc gccgtcatca aggatggtgg
     5161 tcggcccgcg gccattgccc cagtcaagga tgcggtcggt gaagtcccag tactcttcaa
     5221 gactctcccc cttgtaggca tagaccggcg tgcccttcgc cgccactgcc gcggcggcat
     5281 gatcctgcgt ggaaaatatg ttgcagctgg cccagcgcac cttcgcgccc aggacctgca
     5341 gggtctcgat gagcatcgcc gtctggatgg tcatgtgcag cgacccggtg atgcgcgccc
     5401 cgctcagcgg cctggtcttc ccgtattttt cacgaagagc catcagaccg ggcatttcga
     5461 cttcggcgag cttcatttcc ctccgcccga aatcggccag tctgatatcc gccaccttga
     5521 aatctctgcc ttttttattt gccatgttca ttctccacac gtatcggttg caacagggtt
     5581 atcaatctac agggcctgac tggatattca agcccagtct ttgcgattgc gaaagagctt
     5641 ttcatcctcc tgaccgggcg cgatcgagtg ccgcgcgatg ttcaagagga ttccagtcat
     5701 ggaaagcgaa accaggagac ttgatccgcc aaagctgatg aaggggagcg gaagaccctt
     5761 ggtgggcaga cttccggtga ccacgccgat attcagcagc gcctgggccg taatcatcat
     5821 cgtgatgcca aaggccgtca gcttcccgaa actctccgga gcgcagacgc tgatcctgag
     5881 cccgccaatg aagatcatga tgaacagcac cagcaccata agcgacacgg cgaggcccag
     5941 ctcttctcca ataatggcaa agatgaaatc agtgtgggct tccgggagat agaatcgttt
     6001 ctgcacactc tgccccagac cggcgccggt cgctccgccc atcacgaagg catatctcgc
     6061 attgagcagc tgccaggctt cggaagcctc atacttctcc ggattcagaa atgcgatgat
     6121 gcggcgccag cgctcggcat tgtgcatcac ggcgaagaag aaggcggtca gcccgagaga
     6181 acccgcgatc agcagatagc tcagccgcac ccccaccaca aacatcatgg aggcgcccac
     6241 cagcgcaatc agcatggtgg tgccgaagtc cggctccaga aagaccagca gaagcatcgc
     6301 gccaaggaaa accgaaggga tcagcatccc gcggacgaat tcgcgcgcgc gccgctggat
     6361 tcgcgacatc caccaggcca tcagcagaat catggatatc ttcgcaaatt ccgacggctg
     6421 caggctcagg ggccccaggc gcagccaccg gctgctgccc ttcaccgtgg tgccaatgcc
     6481 gggaatcagc gtcagcgcca gcagcgccat tgtcaccagc gcaagcggca cggccagccg
     6541 gcgccagatg cggtagttga tctgcgagag cagcgctcct gcgcacagag aaaggatcag
     6601 ccagaccagc tgtctccgga agaaatactg cgggtcctga tactgttcct ggcccgccat
     6661 cgcgctggcg ctgtacagca taataatgcc cagcgtcagc agaatcagca ccgcgccggt
     6721 aaccagactg gcagtgttcc gcatctacgc gctgttcagg gagccattgc cgacgaactg
     6781 aacgggatca ccagcttctg ccccgcggac agcacggcat cctcagacag tccgttggcc
     6841 gcggccagat cttttttcag cacagcgaac aaacgggcca cgctctccag cgtgtcgccg
     6901 gtggccacgg tgtgggtgaa cagatctccc gcgggtgcag cggaggaagc caccggttcg
     6961 gaaaccttca ccggggcggg ggccggaatt tcctcttcaa ccggaatttc cggctgcggc
     7021 tcgggaactt cctcgatcgg agcaaccgtc acgggagccg gcttgggggc cgcggaagat
     7081 gcggttccgc ccttgggaat gaccagtttc tggccaatgc gaatgctgtt cggattggat
     7141 atctggttgg cggcggccag gtccgcagtc ttcaccccaa aacgcgcggc gattttggaa
     7201 aggctgtcgc cggcggcgac cacataggtg tctcccgaaa gcgttcccgc agcgggtttg
     7261 ggcgcgggtt ttgcggcggc tttttcagaa cgggcatagg cgggcagcag aagtttctga
     7321 ccggcccgga tatcattcat gttcttcaaa ttgttcagtt cggcgatttc gcgcgcggac
     7381 acgccgtggc ggacagcaat cttcgaaagc atgtccccct tcttgaccac atagacgttc
     7441 gcatcgtcat ccgcgggcgc cgcggcgcgc ggcgccgcca cgggggccgg aaataccggc
     7501 tgaggagcct tgatgaccac ccggtcgtcg ggcatgtcac gcggaggcat gacgggcgcc
     7561 ggcgggggtt ccactctcgc cgccgggggc gttccgcacc cctgcatgaa aaacacggag
     7621 ccaatggcta tcacgtgcac tattgttatc acagcaatca acaaaggcgt tttcacaact
     7681 tttctcctct ctgtatgcga ttccatcatt aaattccaag tgacgctatt tctcattttc
     7741 cgtctccttc accatggcgg cagccagccg catgaactgt tctccccgct ccgcaaaatc
     7801 gcggaactga tcataactcg tgcatgcggg cgccagcagg accacctcgc cggggcgggc
     7861 gtctgcccat gccgccctga tggcctgctc cagattcccg cagagtctgc agggaaccgc
     7921 gccactccag gccgaagaca tcttttctga atctcggcct atgagataag cactttgcac
     7981 ccgttttgcc aataaatctt ttatttcctc gaagcgctcc tctttcgcct gcccccccgc
     8041 aatgagccgc actggtttct ttgtcatttt gagcgaagcg gccagggccg caatattggt
     8101 ggctttggag ttgttcacga attccacccc gcgggccgtg cccgccggct gcatccgatg
     8161 aggcagaggt tcaaacaagc gaacggcctc ctccgcaaag cgcaaatcca cggcagcccc
     8221 cgcaaacgcg gctgaaagcg ccgcgagtga agggcccatc acgtcgttgt caaagagtgt
     8281 gcctgtgtaa tccgccaccg tctttccatc atgcacaacc cggccgtcaa gccaccggaa
     8341 ctgcgccgct tcttcggggc caaaagtcac ggccgaccgg ccgcaggccc acgccagcgg
     8401 ggccggaaca atgcagacat ctttggggcg catccgcgcg aacattcgca gcttgagttc
     8461 cagatagtgc tgcatggtcc cgtgccggtc cagatgattg gggagcacat tcagcagaat
     8521 gccgatgtcc gggcggaagc ggcacaccgt ctcaagctga aacgagctga cctcaagcac
     8581 ccagtcgtca gcagagggat cttccgccac ctgttcgcaa aaggacaagc catagttgcc
     8641 ggaaatccgc gcacgacgac cggcccgcgt cagggaatcc gcacagcatt tcaccagtgt
     8701 gctcttcccg ttggaaccgg taatggccca aacgcgcccg cggcagcggc tccagcccag
     8761 ttccagttcg ctgatgcacg gaatcccccg ctccttcagc gaggtcaccc atgaatggtc
     8821 ttccgcaatg ccgggactga ccacggccag gtcgaaggaa ccgcccggaa gtgcgccgca
     8881 gcccgcaaga acatgtgcgc cgtccttctc cagaaaccgc gccgcatcca gcaccggctg
     8941 cgagtcgacc gcggtcacgg gcaccccttc agccagaagc agacgcacgg ctccccggcc
     9001 gctcgctccc agcccaagca ccagcgcatg tttgattttc ttcgtcatct tattttcagc
     9061 gtggccactc cgatcagcgc acagataatg gatacgatcc agaaacgcgt cgtgatcatg
     9121 gtttccacca cctcgagatc ccggttctct tcctcggccc gttttttctc caggaactca
     9181 taatgatgat gcagcggggc gcagaggaat atccgccggc cgcccgtgaa cttgaagtat
     9241 gcgacctgaa gcatcacgct gagcgcttcg gccacaaaaa caccgccgac gatgatcagc
     9301 gtgagctcct gcttgatcag gatggccacc atggctatgc ctcctcccag cgcaaggctt
     9361 cccgtgtcgc ccatgaacac ctttgcggga tggcaattga gccagaggaa gccgagactg
     9421 gcgcccagca gcgccccgca gaacaccgcg agttcaccgc cgcttttcac aaacgggacc
     9481 tgaagatagg ttgcaaactc gtaatggcct gccacataag ccatgaagag ataggccaga
     9541 gcgaccgatc ccgtgcagcc ggcggccaga ccgtccagcc cgtcagtcag attcacggca
     9601 ttggtcgagc ccgcaatgat gagccagatg aaaatcagcg aaccaagcag ccccatgttg
     9661 gtgatcaggg ggtttttcag gaacgggacc atcagattgc gcgtgcggtc tgccgtggcc
     9721 ggatgagaca gcagaaccgc cgccatcaga acaacccagc ccgtctgcag cagcatcttc
     9781 ccgcgcgcgt ccagtccctt ggactgccgc ctgcgcagct tcagatagtc gtcccagaac
     9841 ccgaccccgc ccatgaaaca caatgtggcc agcgtgagct gcacatagag gtttgacggc
     9901 acggcccaga gaagtgtcga cacgaccgcc gcaagaataa tcagcagccc ccccatcgtg
     9961 ggggtgccgg ccttcttccc gtggattttg agaagcgtct cggagtcctc cgtgcggatc
    10021 tgctgcccga ttttaaaccg ccgcagcgcg cggatgagcc aggggcccgc aatcaggctg
    10081 atcatgaacg ccgttcccgc cgcggcaaga gcccggaatg tgatatactg aaatatgcgg
    10141 aggaaggaaa acctctcgct caactgatac aaatagtaaa acatgattac aggctcctgt
    10201 ccttgttgaa caattcaatg aggttttcca gccgcacggt tcgtgaagcc ttcagcagaa
    10261 caatatctcc cgcctgcgcg tcgcgcagcg caatggcggc ttcttccagc ccggcgcatg
    10321 agacctggtt ctcccgggcg aacccggact gcaccgcccc gcggccaata tattcggcca
    10381 gcgggcccac cgtcaccagc cgttcgattc cgctcttcgc aagatactcg ccaattcgac
    10441 ggtgctcacc gggccccgtc ggccccagtt ctcccatgtc gccaagcacc gcccatttgc
    10501 ggcctgaaca ggcttcgccg ctcagtgact gcagggccgc gctcatgctc agggggttgg
    10561 cgttgtaggc atcgttgatg atggtcacac ctgccgcgca aatccggttg ccgcgaaggc
    10621 cgagaggctt gaagcggccg atttgctccg cggctgtgtt caggtccgcg ccaagctggg
    10681 cggcggcggc cgctgaaagc agggcgtttc gaatgaagta ttcaccgggc aggggcattt
    10741 cgcattcacc cccctgttcg cccttgcgat cagcaaacct caccgtctcc ccgcggcggg
    10801 aagccgtgta atccgcgtca gcgccgggcg cgagcgatac tgacaccaca cgcgcacgga
    10861 tcctgctgcg gaaaaattcg taccagggct catcggcgct cagtacaaca aaaccgcttc
    10921 ccgggacgga agccgcaagc accgcttttt cttccgcaat ccgttccact gtgccgaaat
    10981 attcaagatg tgcaatccca accgtggtga taatcgccca ctgcggctgc agcacagcac
    11041 agagctgttt catctctccg atatgattga tgcccgcctc aaacacgcca taacgggcct
    11101 cccgggtcat cgccagcagg ctgagcggga gtccgatgtc attgttccag tttccgcgtg
    11161 tgcgggcggt gggcccgatt gaactcagaa tatccgctgt cagctccttc acggtcgttt
    11221 tccccgcgct tcccgtgacc ccgcccacag aaccgggaag acgggatcgc caggcggcgg
    11281 cgatgtccag cagtgcaagc ctggtgtttt ccacgcccag caaaggcccg cccgccgcct
    11341 gcccggactg aaaatgatca cggtccgcaa ttgctccggc ggcgccctgg gccagggcct
    11401 cattgatgaa cgcatggccg tcatgccgct ccccgcgaat ggcgacatag atttctcccg
    11461 gcttcaactg ccgagagtcc tgacagaaac cggtaatggc ggcggacggg cgggacgacc
    11521 atgatccccc gctccattca gcgagttcat cgggatgaaa agcatgcgcc ttcacgccgc
    11581 ccgctcctct ccgaggattt cgcgcgccac ctggcgatcg tcaaacggaa gcatcgtttt
    11641 cccgacttcc tgaaaagtct catgtccctt gccggcgatc agcacgatat cgccgggctg
    11701 cgccatcgcc agagccttct cgatcgcttt tcgccggtcg ggctccactt catatctgcc
    11761 tttcgcgcag cctgcttcaa tttcgcggat gatggacagc ggatcctcca tgcgcgggtt
    11821 gtcgcttgtg atcagcgcat gatctgccga ggcggtcacg gccgccccca tcagcggccg
    11881 tttggcccga tcgcgattcc ccccgcagcc aaacacggtg atgagccttc caccggtcag
    11941 ctctcgaagc gtggcaagaa cattctcaag cgcggcaggc gtgtgggcat aatccacaat
    12001 cacgctgaag tcccggctgg attcaatccg ctcaagacga ccgggcgcgg attcgaagtt
    12061 ctccagcgct ttctgcataa tgtccaaatc aattcccagg gccccgcagg ccgccacggc
    12121 cgcgagcgca ttgccgacat tgaatccccc cgacaggcga atgcgcatgt cacgatgccc
    12181 ccacggagag atcagatgga aggcgcttcc cgcacggtca gacaggattc tttccgcacg
    12241 aacatccgca tcctcgcgcc cgaaagttat catcggaccg cgccggccaa tctccccgga
    12301 aagccggagt ccccaggggc aggaggtgtt cacaaccgcc acgccccgtt tgcggccttc
    12361 cggcagcgcg tcgaaaagtt tcttcttggc cctgaagtaa ttttcgaaat ccccgtgata
    12421 gtccagatga tcctgtgaaa ggttcgtgaa aaccgccgca tcgaactcaa ggccgaagac
    12481 acggtcctgg tcaagcgcgt gagatgaaac ctccatcacc gcgctgcggc agcccttcct
    12541 gaccatttcc gccagcatct gctggatctc cgcggcatcg ggagttgtcc gcgaagcggg
    12601 aatgacacgc tcgccgattt cataacggac cgtcccgatc aatccgggat gttcccctgc
    12661 ggcctgcagg atcgcactgc acatgaaagc cacagtggtc ttcccgttgg tgccggtgac
    12721 tccgatcatt cgcagcgaat cagccgggcg gccgaagaag atcagcgcgg cgcgggccag
    12781 ggccagcctg gcgctggcga catgaacggc ggccactgtg cgggggacca tggggcgggc
    12841 ctcggaaaca acggcggaag ccccccggcg caccgcctcc tccacatact ggttcccgtc
    12901 ctgactgtgg ccccgaaccg ccacgtacag ccagcccggg cgcaccagtc gcgaatcaca
    12961 ggtgatgccg tgaatctcgg aatgttccgg tcctgaacgg cccagaactg acgcgccctt
    13021 cagcaaatca tccagcagaa cggtatgttc tgtttgtaaa ctcacggcga agcctccatg
    13081 acggacgccc cgcccacaac atgccactcc gccggacggg aggccagata gcgcatgacc
    13141 ggttccgcaa tcttcttgaa gaccggcgcg gccaccacgc cgccggtgtg cagaggctgt
    13201 ggctcatcca ccaccacaat aatgctgatc tcaggattct ctgcggggat aaagccgaca
    13261 aaggacgcca tgtgagcact gtccgaatat ccgccgggaa cgggcttctg agccgaaccg
    13321 gttttccccg ccacggtata ttccgccagc gccgcgcggg aaccggttcc tccctgctcg
    13381 gtcacgcggg ccatcaggcg gcgcatctgc gcggcggttt cctgcgaaat cggccgcgcg
    13441 accacctccg gctcagcccg gtaaataatg cgcccgttgc agtcccgtgc cgtgccgaca
    13501 acatagggcc gcataaggaa tccgtcattc gcgatggcgg atatcagcga aagcatctgc
    13561 atggcggtca cggccacttc gtgtcccatg gcaatgcggg tgacgctgat ctttgaccat
    13621 ctggcgcgtg aatacagcag gccgccctcc tcgccgggaa gatcaatgcc ggtaaaacgc
    13681 cccaccccga aatcgcggag atacttctcc agttttccct ctccgagttt gagggcgatc
    13741 ttcgcggccc caatgttgct ggactttttg atgatatcct gcaccgacag cagcccgtaa
    13801 gggtgatagt cccgaagagg tcttcccccg taatgccaag tcccgttctc gcagtcaaaa
    13861 acctcatcct gcgtgattat cccctcgtta agcgcggctg caatcaccgc caccttgaac
    13921 gtggatccgg gttcataatt aaagctgatg gcgcggttgc ggtgcacctc tggatccacc
    13981 ttcccaaagt ggttcagatc gaaatccggc agactggcca tggccagaat ctccccggtg
    14041 cgcactctct ggacaattgc ccaggctccg gcggcatgat gctcctccat ggcctcgtga
    14101 agcgcctttt ccacataata ctgcacccgg ctgtccagcg tcagatagag gtccgaccct
    14161 tcctgcggag gaatgtccag ggaccggcgg ctgaagattt ccctcttgtg ggcatctttt
    14221 tcgctgattc ggtacccctc caccccgcgc agccagctgt tatagcgctg ctcaacgccg
    14281 ccgctgccct cgccgttcag gttggagaaa cccacgacat gagccatcat ggccccgccc
    14341 ggataattgc gcagagaccc ctcatcgaaa tgcacacagg ggagattcag cgcacgaatt
    14401 tcatctgcct tgtcagccgg aacgaaacgc ttgatgggcg catagtttcc ggttcggccc
    14461 tgcagtctgt tcagcagcag ggccaagtcc agatccagca tgcgagcaag ctgttccgct
    14521 acgtactgca catgcccgcc ggcattgagc tttcccggat caaccttgac atccctcacg
    14581 gcaatatccg cggccatgac cgcgccgttg cggtccatga tgctgccccg gcccacggat
    14641 attttatgct cgaacttgag ggtcttctgg attttaagcc gcagcgcatc gttgggattc
    14701 agatgcagcc agaagagttt tgccgacagt ccgccaaacg ccgcaaggat cagaaccagg
    14761 acgcatgcca gacgccagct gaaaggttgt ttagtcattc atggcaaccc gcgaacggcc
    14821 atgggcatcg tcgtgcaggg ccaatgagga ttcgtccaga ccacccgcat acaggctcgc
    14881 gggaacctca acaatgtgcc ttttgtcagg gaaacgcatt tccagtccat gccggattaa
    14941 agcctgttca acacctggaa gtgttgtcat ggccgcccat ttgatttcct ccaccctgcg
    15001 gcggtcctgc acctcagaat agtgcttttc cagattcttg atttcggttc ccagggcctc
    15061 gcagcggccg cccagccaga gataaaacag ggccgccgag ccaacgaaca gaagtgttcc
    15121 cgccagcgtg aaggggaagc ccgtgttttt tgctccggtg ttcctgctgt gcactttacg
    15181 ctttcttttt ctgctcatct tcagtccttt ccaccacgcg cagcctggct gaccgcgccc
    15241 tcgggttcat tttcatttct tccggaccgg ccgtctgaaa ttttcttcct accagtgcca
    15301 ctctcggtcg ctctccgatc cagtccgctc cgccggcctg cagcgattcc atccgcccgg
    15361 catgcgccct gaagcatctt ttcacccgcc ggtcctcaag gctgtgaaac gaaatcaccg
    15421 ccatgcggcc gtgcggattc agcagcccca atccatcttc caaagcctgt tccagatgct
    15481 caaattcgtt gttcacggca atacgcaaag cctgaaaggt ctgcgtggcc gggtggattc
    15541 ttcccctccg gccgcctttt gcccgctcca caatctcggc aagctgccgt gtgctcttga
    15601 cgggccgcgc cgcgacaatg gctgcggcga tccgccggga ctgccgttct tcgccccatt
    15661 tccagatgag cgccgccagc tctgcttccg gcaggctgtt caccagctcc gcggcggtga
    15721 gtccggaatt gcggtccatc cgcatatcca gggggccttc ctgctgaaag ctgaaacctc
    15781 tctggggctc agccagctga tcggatgaaa cccccagatc cagcaggatg ccgtccactc
    15841 cggagatttt gagatccctg agagcggccc ccatctctga gtaattcctg ttcaccagca
    15901 ccacattgct ccattccgcc aggcgcttcc ggcagcgccg gattgcttca tcatcccggt
    15961 ccattgccac cacgcagccc gtaccggcca gtctctccag tatggcggcc gtgtggccgc
    16021 ccgatccggc cgtcccgtcg agataaatcc ctccgggcct tatcgccagt gcggccacag
    16081 cctcttcgag catcaccggg cgatgaattc ccggtttatc ggctgtgccg ctgatggccg
    16141 atccccttct cagaacagat aattggccag agtccggcca attcgcgccg tgtgtatttc
    16201 cttcggtacg gcttcccagt ccttcatgcc cgccgaaaca acggccgggg cgaggcccag
    16261 ccgggacgcc acggcgcggc ccgcttcagg aatggaaaac tgccggcgcg gacagcgccc
    16321 gccatccaga gaaccggcca gcaaaagcga actacccata ttacccataa aacatctcca
    16381 aacgggctcc cgcggaggag ccaactgtta aaaacctaca tatttagcag cgtctcccag
    16441 cacatccgga ttggcggaag tatccacctt gctccagcgg tcgggatccc ataactcaaa
    16501 gccgtcaaaa gtgcccacaa gcaccacctg gttcgtcaga ctcgcgtagt ccaggaggtc
    16561 atccttgatg cggatccgtc cctgcgcatc ccacgggcac cagtcagagc gagaggcaag
    16621 cgtccgagcg agctgcctgc ccctttcgtc agctatcgaa aggttacgca gcgattccat
    16681 acgacgagtc atttcccggg cagggtaaac acacagacat ttttcttgta
//
LOCUS       DAOQWR010000049        16043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4070_length_16043_cov_5.981736, whole genome shotgun sequence.
ACCESSION   DAOQWR010000049 DAOQWR010000000
VERSION     DAOQWR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16043
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_16043_cov_5.981736"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2201)
                     /locus_tag="PKK36_05620"
     CDS             complement(<1..2201)
                     /locus_tag="PKK36_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94073.1"
                     /translation="MKFARLIVSIVFPVFLTFPSAGQVPEVINYQGRIQVQGTNFTGT
                     GLFKFAIGTEGMGTVYWSNDGTLGGEPAAGIEANLDKGLFSLGLGDTKITGMAALNPG
                     ILAGTDLWIRVWFSDGVLGYEQISPDQQIQSVPYALLAAGVSSLAGHDVTELDDVSDA
                     GSGMIITADERNQIAFNQDGIAALSTNLADEISGRVAADASLAGDLAAASNALNARVT
                     QNTDDISASYSLITNNAGRITALDTAKVNRAGDTMTGALNISSAADAGDLVIGSTGTA
                     VSIGRSASGTGSGTAVGRGANGSGYGAGIGLYADGSENGAAAGYSAKGGWHGAAVGAQ
                     ANGSFYGVAAGRQANGSMAGTAVGYDANAFDRGTALGFGARAMVGGIAIGPGSFGILT
                     NIAIGTDAWAGGIQRITIGNEIQNEVDNSIAVRGSLYLDGGEAVYYRPGAGTGTWVNL
                     LENTATGTPLYVYTETDPVWDAEKAGYATGTPLYVYSETDPVYASEKSGLATGTPVYA
                     ESDPVWESEKSGYATGTPLYVETYTGDFKADGSVPMTGDLNMDDKNLETVNELHVSLI
                     YNEWGTRLLIAPYLGALYGSWSTESNLTVGGSLTLGGVEYTNLATGTPLYVETDPVWE
                     SEKSAYATGTPLYAYTETDPVWESEKSAYATGTPLYVETYTGDFKADGSVPMTGSLDL
                     GGNSVTNIGQIKTGIGFFEASPTRYLCIQGGHAVGETILAMSSPTDAGIIY"
     gene            complement(2403..2759)
                     /locus_tag="PKK36_05625"
     CDS             complement(2403..2759)
                     /locus_tag="PKK36_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94074.1"
                     /translation="MKKRKIRRGFIPFMAGYAAAAMIVSWFLGSRIDEGTLALILFGL
                     VPVVMFAWIHDTLVQGEMWRQGPRSLTAASTLNEQRISRKKQPVKFWTMIALTAVFAA
                     AAAAFVVMGILIKCSV"
     gene            complement(2811..5144)
                     /locus_tag="PKK36_05630"
     CDS             complement(2811..5144)
                     /locus_tag="PKK36_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNR94075.1"
                     /translation="MNVKDLKVVNHSVEKVDAMALALGKSLYVADLKPEDCLHVKMLW
                     SPHAHARIKSIDVSKAEKMKGVHAVLWHGNVPRVAHCTAGQGYPEPSPYDTFMFDTKV
                     RYVGDRVAAVCAETREIAQAAVEAIKVEYEVLEPVLSIWDALKPGAPVIHDEPDCSIP
                     IPVPYEPEKNVVARLEMTVGDVEQAFKDSDFVFDEEYETQYAQHCPMETYVCSAQIDA
                     RNRIVITTSTQVPFHCRRIVAQTLQIPEKQIRVIKPRIGGGFGSKQEILLEDVVAMFA
                     LRTQKPVFFEFTRQENFLTGRTRHPIHFKMKGGVKKDGTITGYSMIATSNTGAYGGHG
                     LTVIGCCGSKVLPLYHIDNLYFKGTVCYTNLPVGGAYRGFGATQAFFGFESQIDMMAE
                     AIGMDPLEFRMKNHIQLGETSPVFSALGEGKEGVPMDISSCALDECIRQGAEEIGWKN
                     RKPHTEKTGRYRRGMGMCCLMQGSSVPEIDMGAASIKMNDDGSFNLLIGATDLGTGSD
                     TVLGQIAAEAIGTTIDKMIVYSSDTDMTPFDVGAYASSTTYLSGEASRKAGAKVNRQI
                     LAVAAEMLDKKPEDLVAANSVVTAKDGSAKVTFAEIAKYSLYQKNQFQIMDTASHITH
                     KSPPPFAAHFVEIEVDTWTGKIRVIKYVATVDCGTAINPKLAEGQTEGGLMNGISYAL
                     TEEYLFNDKGRMINSTLQRYHLFSMRDKPEVKAILVPSWETTGPYGAKSVSEICINGA
                     GPGINNALYNACGIRLHTMPFTPEKVWKALQEKEKKA"
     gene            complement(5141..5614)
                     /locus_tag="PKK36_05635"
     CDS             complement(5141..5614)
                     /locus_tag="PKK36_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR94076.1"
                     /translation="MREPKHTISLTINGQKKKFKVNAEEKLLDVLRREGYKSVKFGCG
                     EATCGSCTVIMDGKAVYSCILFAFQADGHDIWTVEGIGTFDKPHPLQRALVDEGAVQC
                     GFCIPGMIMSAKAMMDANPKPEEEEFREKMDGNLCRCTGYEKIWVAIQKVIGGAK"
     gene            complement(5616..6455)
                     /locus_tag="PKK36_05640"
     CDS             complement(5616..6455)
                     /locus_tag="PKK36_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNR94077.1"
                     /translation="MKFCDFIIPHTVEEACGILRQLGEAAQPIAGGTAFHFYTDDRIE
                     TAIDLSRLELNGITPADGGFRIGAMTTLDQMTGFKADGWVLGEVARHISTQQIRNIST
                     IGGNISRVFPWSDMAIALQALGAVMHVQSDSAQDIPADEFFKSQPARLFKDGALLVGV
                     TVPAVKTPCGFGWRKEQRTNKGFGLMTSGIMLTLGGNSISGIRIAIGAGVPFPRRLPK
                     LEAALTGKPADEKTLGDIILSRPEEVNWIAKEGLSEEYIAHLADVVIYDALMQAVRRA
                     KGE"
     gene            complement(6493..7890)
                     /locus_tag="PKK36_05645"
     CDS             complement(6493..7890)
                     /locus_tag="PKK36_05645"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNR94078.1"
                     /translation="MAKILLKNVNTLVTMVPGEKPLHNVDLLISGKKIEAIGPNLQIP
                     EGARVRTIDGTNRVVYPGLVNTHHHLYQTLTRNLPKVQNAKLFDWLVGLYEVWRELTP
                     EAVEVSTQVGLGELLLSGCTTTTDHFYVFPQSAPEEFLDVEIDTARRMGVRFHPTRGS
                     MSRGKSCGGLPPDDVVQSPEAILKDCDRLIAKYHDPKPFAMCRIVLAPCSPFSVTTEL
                     MKETAAYARTKGVFLHTHLCETKDEESFTMQVLGKRPLAYMEECGWVGPDVWYAHGIY
                     FNDDEIKRLAETGTGVAHCPGSNLRLGSGICKVPQLLREGVRVGLAVDGSASNDSSSM
                     TREMQLALNVHRVGTGVDQMPPQDVLKIATTGGASILGQPEIGQLKPGMAADIAMYRL
                     DRVEYAGAMHDPASAILFCGAGARADYTIVNGKVLVAKGKLVGLDEEALFARANEVAA
                     KMVENAERKTGVSYR"
     gene            complement(7995..9152)
                     /locus_tag="PKK36_05650"
     CDS             complement(7995..9152)
                     /locus_tag="PKK36_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94079.1"
                     /translation="MIGGLLGEILFQALLFFIILRLIAKHEADYDFGKLAMLSAVITM
                     CTTVLTLLAADRGLIYIPAGYTAAVVVATWLLVKFCWVPVWKALIAVVTVIALKAVVR
                     FLILMLLARALGGPAAGTPAAAAAPAAPAEKRASAQTPDEVRLREIHGWLRWQVSLSE
                     QLAAAKKICDSQTPPVEITGSTALVVAGSDGKVPVRSGRLQVTGRAGGNGVENAAAVL
                     NALSEHAAVKPDGIKVHPQRMVQDPVDARFTIFTLNIAYPPRFLLADAPPALEPAAEE
                     AAGMILPAGDPATAASNAVAAAATAAGFQLTRLSPLPVFSAPGPEGKIALLPVLIAGR
                     GPWNSVIEILRALESNRGLQIEKMALRPLTNGKDWEMSVRFEQAAWKPEGP"
     gene            complement(9185..10324)
                     /locus_tag="PKK36_05655"
     CDS             complement(9185..10324)
                     /locus_tag="PKK36_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNR94080.1"
                     /translation="MENFSYFNPTRIVFGKGSIARLPELIPSTARILLTCGGGSIKRN
                     GVYDQIKAALKDRAVLEFSGIEPNPQFATCMKAVEIVKAEKIDFLLAAGGGSVLDGTK
                     FIAAAARFGGTDPWDILTGASVTDAVPMGAVLTLPATGSESNGTAVISNSQLNEKLFF
                     GSELTYPKFSILDPEVTFTLPAGQTANGIVDAFVHVMEQYMTFAVNAPLQERQSEAIL
                     TTLIEEGPKVLENPNDYNARANIMWCACQALNGLIACGVPQDWASHMIGHELTALYGI
                     DHAQSLAIVLPGVLKHQVESKRDRLLQCGQRVFGMRTKRPESVIRKIESFFLSLGVKT
                     RLKDYNIPARAAKIVAQRLAARGMRLGEKQDIDADAISAILKMRR"
     gene            complement(10465..11691)
                     /locus_tag="PKK36_05660"
     CDS             complement(10465..11691)
                     /locus_tag="PKK36_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNR94081.1"
                     /translation="MAKKTEQITGNMLIAQSGGPTMVINQSLIGAVLEAKKHKNIKGI
                     YGSLHGIQGILKEEFIDLRKESVSNLEAVAQTPSSALGSVRKKPKPEDCVKLFAVMKK
                     YNIRFFFYIGGNDSAETTHIVNEEAKKAKYELRCFHIPKTIDNDLRENDHTPGFASGA
                     KFVAMAFMGDNLDNRALQGVKINVVMGRHAGFLTAAAALARVYPDDGPHLIYLPERVF
                     SMDKFARDVKRVFKKHGRCVVAVSEGISDKDGTPIAALLTAEVEVDSHGNVQLSGSGA
                     LGDRLSDYIKNKTQISRVRADTFGYLQRSFPGIASKVDAKEARETGALAVKLAATKDT
                     DGSIAIKRKAGKKYAVTYKRVPLKNVAKETRHMPDSFISKAGNDVTRAFINYAKPLVG
                     ELPKIGRFKGVRVKKK"
     gene            complement(11861..12319)
                     /locus_tag="PKK36_05665"
     CDS             complement(11861..12319)
                     /locus_tag="PKK36_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNR94082.1"
                     /translation="MLNEDYKEMLQSLVDENVKFLLVGAYALAAHGYPRSTMDIDIWV
                     RPSPENARAVLRALSKFGAALHNLSSTDLQKSDTVFQIGVAPRRIDIITGASGLEFDG
                     AYSRSIETDIDGLKINIPSLDDLICNKRASARAKDIADAEALEKLKHSEQ"
     gene            complement(12586..13215)
                     /locus_tag="PKK36_05670"
     CDS             complement(12586..13215)
                     /locus_tag="PKK36_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNR94083.1"
                     /translation="MQGLRTSLAWLVLGIWFTQQIVRADLIYFRPDEPVQVGLSTWDL
                     DINDDAVVDLVFSGSPSEFECFTEGSNLLLGIPPSPPDTGHYLYPLDPGYEIDSSPTA
                     PIEWGTSPYGCLFASYQEGAGGWTGIGYWREENADGYLGVQFDIDGNTHYGWVHVINT
                     YGITGEIVEWAYESNPNTGLQAGVIPEPTTLLLLLTGTGVLVLRKKKIR"
     gene            complement(13225..15714)
                     /locus_tag="PKK36_05675"
     CDS             complement(13225..15714)
                     /locus_tag="PKK36_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR94084.1"
                     /translation="MKPNKVVERYARKLAPLTTDVSEKRKNMDASALLVGVSSTRREK
                     TMQTRMIAGVARLVLAASVFVGMTTAQAQSTDRTDEYRAEIASIEHFNEVQREADMPE
                     MEIPTYEAWLKAKQETEANALTSADSVAEPDEPTKQERMRQWLNKGVRTADDQPSSIA
                     DAEMKRRERLWAKSSQLLKAHQQEMEQAREWAEQHGFAESARLPEGGVMGLVGIRNGI
                     PKYNVTFNAGAADTISVDEVWPGGGSGLDVSGANTLLGIWDGGDVLTNHYEFTSGGAT
                     SRAVDQDGTSSLPVDLHPTAVAGTMTAAGVAYSGDPRGMAYQAQLWTYDWVNDIEAEM
                     NSAYANDLHVSNHSYGEQAGWGFITVYTPTPVYTNCWWGDIAISPNESHYFGEYDSDV
                     TNTDALAYGNPYSLPVWAAGNDRDDAAPTDGRWYITFSNTVPIWSNTSRPNDYYQNGY
                     DTVSDHGVAKNVLTVGAVYKISGGYGGTSSVSIAAFSCFGPTDDGRIKPDVVAAGVDL
                     TTPVRNPADPNNPYYYVLSTGDTNNPSYATGTSFSSPSAAGALGLLVDLREQLSPELP
                     YWASTLKTIITHTADEAETTGPDYKTGWGLINAEKAALLIQQDYDDGGKQYIKEVLLN
                     DGDYIEFPVIATGGVPVTVSIGWTDPAGTSPAESLDPTNSVLVNDLDLRIISSSGVTN
                     FPWVLDPANPSYAATTGDNVRDNTEQVVVTNPPAEAVYTVRITHKGTLVDDTGTNSAQ
                     AVSIILSGILPESRSQTQIAEFLVAGDDELVGWPSVVGQNYRVQTATNLLVQSWTNLS
                     AEISSTKTNTTWESETAPTGEIRFYRLIDTN"
BASE COUNT         3084 a         4666 c         4585 g         3708 t
ORIGIN      
        1 aaatatatta tccctgcatc agtcggactc gacatcgcta aaatagtttc tccgacggcg
       61 tgtccgcctt gaatacataa atatctggtg ggagatgcct caaaaaatcc aataccagtt
      121 tttatctgtc caatattcgt cacagagttc ccgcccaagt ctaggctccc cgtcatcgga
      181 acacttccat cagctttgaa atcgcccgtg tacgtttcca cgtagagggg cgttcctgtt
      241 gcataagctg atttttctga ttcccagact gggtctgttt cggtgtaagc gtagagcggc
      301 gtgccggtcg cataggcgga cttttcagac tcccacactg gatcggtctc gacataaagc
      361 ggggtgccgg tggcaagatt ggtatattcg actcccccca acgtaagaga cccgccgacc
      421 gtaaggttag attcggtgct ccatgatccg tacaatgcgc ctaaatatgg agcgattaat
      481 agtcttgtcc cccattcgtt gtatatcaag gaaacgtgaa gttcattcac cgtttcaaga
      541 ttcttatcgt ccatgttgag gtcgccagtc atcggcacag agccatccgc tttgaagtcg
      601 cccgtgtacg tttccacgta gagcggagtg cccgtggcgt agcctgactt ttcgctctcc
      661 catacgggat cgctttccgc gtagacgggg gttcccgtgg ccagtcccga cttctccgag
      721 gcatagaccg gatccgtctc gctgtacaca tacagaggag taccagtcgc atagcctgct
      781 ttctcggcat cccaaaccgg atcagtttcc gtatagacat acagtggcgt gccggtggcc
      841 gtattttcca gcaggttgac ccatgttccg gtgcccgcgc cggggcggta atacacagct
      901 tctccgccat ccaggtacag actgccgcgc accgcaatgc tgttgtccac ttcgttctgg
      961 atttcattcc cgatggtgat gcgctggatg ccgcccgccc acgcgtcggt accgatggct
     1021 atattagtca ggatgccgaa agaacccggc ccgatggcaa tgcctccgac catcgccctt
     1081 gcgccaaacc ccagtgcggt tccccggtcg aatgcgtttg cgtcataacc cactgccgtg
     1141 ccggccatgg atccattcgc ctgccgcccg gcggccactc catagaacga cccgttggcc
     1201 tgcgcgccca ctgcggctcc atgccatccg ccttttgcgg aataaccagc ggctgctccg
     1261 ttttcggagc catcggcgta taaaccaatg cccgcgccat acccggagcc gttggccccg
     1321 cgcccgacgg ccgttccgct cccggttcca ctggcgctgc gcccgattga tacggcggtt
     1381 ccggttgagc caatgaccag atcacccgcg tccgccgcgg aactgatgtt cagcgcgcct
     1441 gtcattgtat cgccggcccg gttcaccttg gcggtgtcca gtgccgtgat gcggccggcg
     1501 ttgtttgtga ttagagaata actggcggag atatcatcag tattttgagt cacgcgggca
     1561 ttcagcgcat tgctggcggc ggccaggtcg ccggccagtg aagcgtccgc cgccacgcgg
     1621 ccggaaattt catccgcaag gtttgtactc agcgcggcga tgccgtcttg attaaatgcg
     1681 atttgatttc tctcgtcggc ggtaatgatc atgccgctgc cggcatcgct cacatcatcc
     1741 agttctgtca catcatgtcc agccagcgaa gatactccgg ccgcaaggag cgcataggga
     1801 acggattgaa tctgctggtc gggcgaaatc tgctcataac ccagcacgcc gtcactgaac
     1861 cagacgcgaa tccacaaatc cgtgccggcc aggatgcccg gattgagcgc agccattccg
     1921 gtgatttttg tgtctcccag ccccaggctg aagaggccct tgtccagatt tgcctcgatg
     1981 ccggcggcag gctcgccgcc gagcgtgccg tcattgctcc agtatacggt gcccatgccc
     2041 tcagttccga ttgcaaactt gaacagcccg gttcccgtga aattggtgcc ctgcacctgg
     2101 atacggccct ggtaattgat cacttcggga acctggccgg cggaggggaa agtcagaaaa
     2161 acgggaaaaa caatcgatac gatcaacctt gcaaacttca tgattcatct cctcgggatg
     2221 actggcactt caaaccggaa agtatagaag cattggcgac agtcccgcca gaaaaaatcc
     2281 cgaaaaaggt tttacccttt catcaaaagt tccaatccct ggaacttttt cccgaaaact
     2341 tccagtggct ggaagttttc gggcggagag gatgtcagcc ttttgggacg ggctgcggca
     2401 ttttagaccg agcacttgat gagtattccc atcacaacaa aggcagcagc cgctgctgca
     2461 aataccgctg tgagcgcgat catggtccag aatttcacgg gctgtttctt tcgggatatc
     2521 cgctgttcat tcagggttga cgccgcggtg agcgagcgcg ggccctgccg ccacatctct
     2581 ccttgaacga gcgtgtcatg gatccatgcg aacatcacaa ctggaacaag accgaaaaga
     2641 atcagggcga gtgttccttc atcaatcctg cttcccaaaa accaggaaac gatcatggcc
     2701 gccgccgcgt agccggccat gaacggtata aacccccgtc gtatttttct ctttttcatt
     2761 ataaaaaacc gccgcgccgt ttcgggcgcg gcggttttct ttcgcgatga ttacgctttt
     2821 ttctcttttt cctgcagggc cttccacact ttttccggtg tgaacggcat ggtgtgcagc
     2881 cggatgccgc aggcgttgta gagcgcgttg ttgattccgg gtcctgcgcc gttgatgcag
     2941 atttcggata cgctctttgc gccgtagggt ccggtggtct cccagctcgg caccagtatc
     3001 gccttcacct cgggcttgtc cctcatcgag aagaggtgat agcgctggag ggtggagttg
     3061 atcatccggc ccttgtcgtt gaacaggtac tcctccgtca gcgcgtagga gatgccgttc
     3121 atcaggccgc cctcggtctg gccctcggcc agcttcggat tgatggcggt gccgcagtcc
     3181 acggtggcca cgtatttgat cacccggatt ttcccggtcc aggtgtccac ttcaatttcc
     3241 acgaagtggg ccgcgaacgg gggcggggac ttgtgcgtga tgtgcgaggc cgtgtccatg
     3301 atctggaact ggtttttctg gtagagcgaa tacttcgcga tttccgcgaa ggtcactttc
     3361 gcgctgccgt ccttcgccgt caccacggag ttggctgcca cgaggtcttc cggtttcttg
     3421 tccagcatct cggccgcgac cgcaaggatc tgcctgttca ccttcgcgcc ggccttgcgg
     3481 gaggcttcgc cgctcaggta ggtcgtgctg gaggcgtacg cgccgacatc gaacggagtc
     3541 atgtcggtgt cggacgagta gacgatcatt ttgtcaatcg tcgttccgat ggcttctgcg
     3601 gcgatctgcc cgaggacggt gtcgcttccg gtgccgagat cggtggcgcc gatcagcagg
     3661 ttgaaggatc cgtcatcgtt catcttgatg gaggccgcgc ccatgtcgat ttccggcacg
     3721 ctggatccct gcatcaggca gcacatgccc atgccgcggc ggtagcgccc ggtcttttcg
     3781 gtgtggggtt tgcggttctt ccagccgatt tcctcggcgc cctgccggat gcactcgtcc
     3841 agcgcgcagg agctgatgtc catcggcacg ccttccttgc cttcgcccag cgcggagaaa
     3901 accggggagg tttcgccaag ctggatgtga tttttcatcc ggaactcgag gggatccatg
     3961 ccgatcgcct cggccatcat gtcgatctgc gactcgaatc cgaagaaggc ctgcgtggcg
     4021 ccgaacccgc ggtaggcgcc gccgaccggc aggtttgtgt agcacaccgt ccccttgaag
     4081 tagaggttgt caatgtgata cagcggaagc actttgctcc cgcagcagcc gatcacggtc
     4141 aggccgtggc cgccgtacgc tccggtgttg gaggtggcga tcatgctgta gcccgtgatg
     4201 gttccgtcct tcttcacgcc gcccttcatt ttgaaatgaa tcggatggcg cgtgcggccg
     4261 gtcaggaaat tttcctggcg cgtgaattcg aagaacacgg gcttctgcgt ccgcagcgcg
     4321 aacatggcca cgacatcctc gagcagaatc tcctgcttgc tgccgaagcc gccgccgatg
     4381 cggggcttga tcacccggat ctgcttctcc gggatctgca gggtctgcgc gacaatacgc
     4441 cggcagtgga agggcacctg ggtgctggtg gtgatgacga tgcggttgcg cgcgtcaatc
     4501 tgggccgagc agacataggt ttccatcggg cagtgctgcg cgtactgcgt ttcatactcc
     4561 tcatcgaaca cgaagtccga atccttgaac gcctgttcga catcgccgac ggtcatttca
     4621 aggcgggcaa cgacgttctt ctcgggctcg tagggcaccg gaatcggaat cgaacagtcc
     4681 ggctcgtcgt gaatgacggg cgcgccgggc ttcagggcgt cccagatcga gagcacgggc
     4741 tccagcacct cgtattcgac cttgatggcc tcgacggcgg cctgggcgat ttcccttgtt
     4801 tccgcgcaga cggcggcgac gcggtcgccg acatagcgga ccttggtgtc gaacatgaag
     4861 gtgtcgtacg gggagggctc gggatatccc tggccggcgg tgcagtgcgc aacgcgcggg
     4921 acattgccgt gccagagcac cgcatggacg cctttcatct tttcggcctt tgaaacgtca
     4981 atggacttga tgcgcgcatg cgcatggggg ctccagagca tcttgacatg aagacagtct
     5041 tcgggtttca ggtcggccac ataaagggac ttgcccagcg caagcgccat ggcgtccact
     5101 ttttcaacgg aatgattgac gactttcaga tccttgacgt tcatttcgca cctccaatga
     5161 ccttctggat ggccacccag attttctcgt aaccggtgca gcggcacagg ttgccgtcca
     5221 tcttctcgcg gaactcttcc tcctccggct ttggattggc gtccatcatg gccttggcgg
     5281 acatgatcat gccggggatg cagaacccgc actgcacggc gccctcatcc accagcgcgc
     5341 gctgcagcgg atggggcttg tcgaaggttc cgattccctc aacggtccag atgtcgtgtc
     5401 catcggcctg gaaggcgaac aggatgcagg aatagacggc tttgccgtcc atgatgacgg
     5461 tgcaggaccc gcaggtggcc tcgccgcagc cgaacttcac gctcttgtag ccttcgcggc
     5521 ggagcacatc gaggagtttt tcctcggcgt tgaccttgaa cttcttcttc tgaccgttga
     5581 tggtgaggct tattgtatgt tttggttcac gcatgtcatt ctcctttcgc gcggcgcacg
     5641 gcctgcatca gggcgtcata aatcacaaca tccgccagat gggcgatgta ttcctcgctg
     5701 aggccttcct tggcaatcca gttgacctct tcggggcggg acagaatgat gtcgccgagc
     5761 gtcttttcat cggccggttt ccccgtcagc gcggcttcga gcttgggcag gcggcggggg
     5821 aagggaacac cggcgccgat ggcgatccgg atgccggaaa tcgaattccc gccgagggtc
     5881 agcataatgc cggaagtcat caggccgaaa cccttgttgg tgcgctgctc cttgcgccat
     5941 ccgaaaccgc agggcgtttt aacggccggc acggtcacgc ccaccagcag cgcgccgtcc
     6001 ttgaacagcc tcgccggctg cgatttaaaa aactcgtccg cgggaatgtc ctgcgcgctg
     6061 tcgctctgga catgcatcac ggcgccgagc gcctgcaggg cgatcgccat gtccgaccac
     6121 gggaacacgc gcgaaatgtt tccgccgatg gtcgagatgt tgcggatctg ctgcgtggag
     6181 atatgccgtg ccacttcgcc gagcacccag ccgtcggcct tgaagccggt catctggtcg
     6241 agggttgtca tggcgccgat gcggaagccg ccgtctgccg gggtgatgcc gttcagttcg
     6301 agacgggaaa ggtctatggc tgtttcgatg cggtcatcag tatagaagtg gaaggcggtt
     6361 ccgcccgcga tgggctgcgc cgcttccccc agctgcctga ggattccgca ggcctcttcg
     6421 accgtgtgcg gtatgataaa atcacagaat ttcatctgtt ctccttctgt tgtttaactg
     6481 tctgtttcaa attcagcggt aggaaacgcc cgttttgcgc tcggcgtttt caaccatctt
     6541 tgcggccact tcgttggcgc gggcgaaaag cgcttcctcg tcaaggccga ccagttttcc
     6601 cttcgccacc agcaccttcc cgttgacgat ggtgtagtcc gcgcgcgcgc ccgcgccgca
     6661 gaacaggatg gcggaggccg gatcgtgcat ggcgccggcg tattcgaccc ggtccagccg
     6721 gtacatcgcg atatcggccg ccatgccggg cttgagctgg ccgatttcag gctgaccgag
     6781 aatgcttgcg cctccggtgg tggcgatttt cagcacatcc tgcggcggca tctggtccac
     6841 gccggttccg acgcggtgca cgttcagagc gagctgcatt tcgcgggtca tgctggagga
     6901 atcgttgctg gcgctgccgt ccacggccag tccgacgcgc acgccttcgc gcaggagctg
     6961 cggcaccttg cagatgcctg acccgagccg caggttggag ccggggcagt gcgccacgcc
     7021 ggttccggtt tcggccagcc gcttgatctc atcgtcgttg aaatagattc cgtgggcgta
     7081 ccagacgtcg gggccgaccc agccgcattc ctccatatag gccagcggac gctttccaag
     7141 cacctgcatc gtgaaggact cttcatcctt tgtttcgcag aggtgtgtat gcaggaaaac
     7201 gcctttggtg cgggcatagg cggcagtttc cttcatcagc tccgttgtca ccgagaatgg
     7261 cgagcatggg gccagcacaa tccggcacat cgcgaagggc ttcggatcgt gatatttggc
     7321 aatcagccgg tcgcagtctt tcaggatggc ttccgggctc tggaccacat cgtccggcgg
     7381 caggccgccg caggacttgc cgcggctcat gcttccgcgg gtcggatgaa agcgaacgcc
     7441 catcctgcgg gcggtgtcaa tctcgacatc cagaaactcc tccggcgcgg actgcgggaa
     7501 aacatagaaa tgatcggtgg tggtcgtgca gcccgacagc agcagttcgc cgagcccaac
     7561 ctgcgtgctg acttcgaccg cctcgggggt cagctcgcgc cagacttcat aaaggccgac
     7621 cagccagtcg aacagtttgg cgttctgcac cttgggcagg ttgcgggtaa gcgtctggta
     7681 caggtgatgg tgggtgttga ccagccccgg atagaccacg cggtttgttc cgtcaatggt
     7741 ccggacccgc gctccttccg ggatctgaag attcgggccg atggcctcga ttttcttccc
     7801 cgaaatgagc agatccacgt tgtgaagggg cttttcgccc ggaaccatgg tgaccagagt
     7861 gtttacattt ttcagcagta tttttgccat tgctgctcct gtgctttttt ttgcgcggac
     7921 agaatgtccc gtttcgggcc aaaaatccag cccgtttttc tgctaattgc ggttcggggc
     7981 ccttcggcgg ggtcctatgg cccctcgggt ttccatgcgg cctgctcgaa ccggacggac
     8041 atttcccagt ctttcccgtt cgtcaacggg cgcaaagcca ttttttcaat ctgcaggccg
     8101 cgattgctct ccagcgcgcg caggatttca atcacgctgt tccatggccc ccgcccggca
     8161 atcagcacgg gaagcagtgc gatcttccct tcgggtccgg gtgcggaaaa tacgggaagc
     8221 ggactcaggc gcgtcagctg gaatcctgcg gcggtcgcag cggccgcaac cgcgttggac
     8281 gcggccgtcg ccgggtcgcc ggccggcaga atcattccgg cggcttcttc ggccgccggc
     8341 tccagcgccg gcggggcatc cgcaagcaga aaccgcggcg gataggcgat gttcagcgtg
     8401 aagatggtga atcgggcgtc caccggatcc tgcaccatgc gctgcgggtg aaccttgatg
     8461 ccatcgggtt taactgccgc gtgttcggaa agggcgttca ataccgccgc ggcgttttcg
     8521 acgccgtttc cgcctgcccg gcccgtaacc tgaagccggc cgctgcgcac gggaactttc
     8581 ccgtccgatc ccgccacaac cagggctgtg ctcccggtga tctcaaccgg aggggtctgg
     8641 ctgtcgcata tctttttcgc ggcggcgagc tgttcgctca gcgaaacctg ccagcggagc
     8701 catccgtgga tctcgcgaag gcggacttca tcgggtgttt gcgccgaagc ccttttctcg
     8761 gcgggcgcgg caggggccgc tgcggcggcc ggagttccgg ccgccggccc gcccagggcc
     8821 cgggccagaa gcatcaggat gaggaatcgg acgaccgcct tcagggcgat gaccgtgacc
     8881 acggcaatca gcgccttcca gaccggcacc cagcagaact tgaccaggag ccaggtggcc
     8941 acgaccaccg cggcggtgta gccggccggt atgtagatca gcccccggtc ggcggcaagc
     9001 agggtcagca cggttgtgca catggtgatg acggccgaaa gcatggccag cttgccgaag
     9061 tcatagtcgg cctcgtgttt tgcgatcagg cgcagtatga tgaaaaaaag cagggcctga
     9121 aacagaattt cgccgagaag tccgccaatc atgtctattc tccctggata tgctttgcgg
     9181 cagtctatcg ccgcattttc agtatagcgg atatggcgtc cgcatcaata tcctgttttt
     9241 ctccgagcct cattccgcgg gccgccaggc gctgcgcgac aatctttgcg gccctggcgg
     9301 gaatgttgta gtccttcagc cgggttttca cccccagcga caggaaaaac gactcgattt
     9361 tgcgaatcac gctttccggt ctttttgtcc gcattccgaa cacgcgctgt ccgcactgga
     9421 gcaggcggtc gcgcttggac tccacctgat gcttcagcac gcccggcagg acaatcgcaa
     9481 gcgactgggc atggtctatg ccgtagagcg cggtgagttc gtgtccgatc atgtgcgagg
     9541 cccagtcctg cggcacgccg caggcgatca gcccgttcag cgcctggcag gcgcaccaca
     9601 tgatgttggc ccgcgcgttg tagtcgttcg gattctccag aacttttggg ccttcctcga
     9661 tcagtgttgt gagaatggcc tccgactgcc gttcctggag cggggcgttc acggcgaatg
     9721 tcatgtactg ctccatcaca tgcacaaaag cgtccactat gccgttggcc gtctggccgg
     9781 cgggcagggt gaaggtgact tcgggatcga gaatggagaa cttcggataa gtcagttcgc
     9841 ttccgaagaa cagcttttcg ttgagctgac tgttggagat cacggccgtg ccgttggatt
     9901 ccgatcctgt cgccgggagg gtcagcacgg cgcccatcgg gacggcatcc gtcaccgatg
     9961 ccccggtcag gatgtcccat ggatcggtcc cgccgaagcg cgccgccgcc gcgatgaact
    10021 tggtgccgtc cagcacggag ccgccgccgg cggcgagcag gaaatcaatc ttttcggcct
    10081 tcacaatttc caccgccttc atgcaggtgg cgaactgcgg attgggctcg atgccgctga
    10141 attccagaac ggcgcggtcc ttcagggccg ctttaatctg gtcatagact ccgttgcgtt
    10201 tgatcgagcc tccgccgcag gtcagcagaa tgcgggcggt ggagggaatc agctccggaa
    10261 gccgggcaat ggagcctttt ccaaaaacga tccgcgtcgg attgaaatat gagaaatttt
    10321 ccatgaaatg tctcctgatg ctttccaatc aaaaaacggc aaaaaatgcc aaaaaacgct
    10381 caaaaacggg caaaaatcgt caaaaatggc ccaaaaacgg ccaatttttc aaaaaaaggc
    10441 ccaatttacg atttattccg atttctattt ctttttaacg cgaacgccct tgaaccggcc
    10501 gattttcggc agttcgccga ccagcggttt cgcgtagttg atgaacgctc tggtcacatc
    10561 atttccggcc ttgctgatga agctgtccgg catgtggcgg gtttcctttg cgacgttctt
    10621 cagaggtacg cgcttgtagg tcacggcata tttctttccc gccttgcgct tgatggcgat
    10681 gcttccgtcg gtgtctttcg tggccgccag cttgaccgcc agcgcgccgg tttcacgggc
    10741 ctcttttgcg tcgactttcg aggcgatgcc ggggaacgaa cgctgcagat atccgaaggt
    10801 gtctgcgcgc acgcgcgaga tctgcgtctt gttcttgatg tagtccgaaa ggcggtcgcc
    10861 cagcgcgccg cttccgctga gctgcacgtt gccgtgcgag tccacctcga cctccgcggt
    10921 cagcagcgcc gcaatcggcg ttccgtcctt gtcggaaatt ccttccgaga ccgcgaccac
    10981 gcagcgaccg tgctttttga agacccgttt cacgtcgcgg gcaaacttgt ccatgctgaa
    11041 cacccgctcg ggcagataga tcagatgcgg tccgtcgtcg ggatagacgc gggccagcgc
    11101 ggcggcggcc gtcaggaagc cggcgtggcg gcccatgacc acgttgattt tgacgccctg
    11161 aagcgcgcgg ttgtccaggt tgtcgcccat gaaggccatg gcgacgaact ttgcgccgct
    11221 ggcgaagccg ggcgtgtgat cgttctcgcg caggtcgttg tcaatggtct tgggaatgtg
    11281 gaagcagcgc agttcatact tcgctttttt tgcctcctcg ttgacgatgt gcgtggtctc
    11341 cgcggagtcg ttaccgccga tatagaagaa gaaacgaatg ttgtatttct tcatcaccgc
    11401 aaacagtttt acgcagtctt ccggcttcgg tttcttgcgc acagatccca gcgcggaaga
    11461 gggcgtctgc gcgaccgcct caaggttgct cacactttcc ttgcgaagat caatgaactc
    11521 ttccttcaga atgccctgga tgccgtgcag tgagccataa atccctttga tattcttgtg
    11581 cttcttcgcc tccagcaccg ctccgatcag gctctggttg ataaccatcg tcggcccgcc
    11641 gctctgcgca ataagcatgt tgcccgtaat ctgttcagtt ttctttgcca tttttcctcc
    11701 aagagtgaaa ttccttctgc aaatcagcga gaatgaaata acaggttccg cgtaaaatat
    11761 gcaaggccag atgcagccat tggatccaaa gtgcgtccgg gggaacgtcg gggatgagtc
    11821 tcgggatgaa ccacccggcg gagaacccgt agactccggc tcattgttcg gaatgtttca
    11881 gtttttccag tgcttcggcg tctgcaatat ctttagctct tgccgaagct cttttgttgc
    11941 agatgaggtc atcaagagag gggatattaa tcttcagccc gtcgatatct gtttcaatag
    12001 atcgactgta ggccccatca aattccaaac cagaagcccc ggtaattatg tcgattcttc
    12061 tgggagctac tccaatttga aataccgtat cactcttttg aagatctgtg gatgacaggt
    12121 tgtgcagggc ggccccaaac ttgcttaaag cccttaaaac ggcccgtgca ttctcgggag
    12181 aaggccgtac ccagatgtca atatccatgg ttgaccgggg atagccatgc gctgccaggg
    12241 cgtaagcgcc aacaagaagg aatttaacgt tctcgtcgac taacgactgc aacatttctt
    12301 tgtagtcttc gtttagcatc gtatttcttg cttaaagagg tcagctcttg ggtaagaggc
    12361 catacaagag cgagtctgga acttggagat cccggcacaa agccattgtc atcaaccatt
    12421 ccaagcctgc ccagtttagt cacgctctta tccatgaggt aataatacca cgatttgttt
    12481 tagcatcaat tgtttcttta cttccgacgt gcgtccggag gaacgtcacg gttcaccgtc
    12541 aggggtgaag cgccagcgga acccctgtac gtgtgaagcc gattgttagc gaatcttctt
    12601 ctttcggagt accagcactc ccgttccggt aaggagcagt agaagtgtcg ttggctcggg
    12661 gataactccc gcttgaaggc ccgtattggg gttggattca tatgcccact cgacaatctc
    12721 tccggtgatg ccgtaagtgt tgattacgtg aacccagcca taatgagtat taccgtcgat
    12781 atcgaactgt acgccaagat atccgtccgc gttttcctcc cgccagtaac cgataccagt
    12841 ccagccaccg gcgccctctt gataggatgc gaaaaggcat ccatacggtg aagtccccca
    12901 ttcaattggc gcggtcgggc ttgagtcgat ttcatagcca ggatcgagag gatacagata
    12961 atggccggta tctggcggtg aaggagggat gcccagcagc aggttgcttc cctctgtgaa
    13021 acactcaaac tccgagggcg agccactgaa tacaagatcg acaacggcat cgtcattgat
    13081 atccaagtcc cacgttgaca gcccgacttg cacgggctcg tccgggcgga aatagatcaa
    13141 gtccgcccgg acgatttgtt gcgtgaacca gataccgaga accagccacg caagcgatgt
    13201 acgcaagccc tgcatggtgg ttctctagtt cgtgtcaatc aatcggtaga aacgtatctc
    13261 ccccgttggg gctgtttcgg attcccatgt agtattagtc ttggttgagc tgatctcagc
    13321 gcttagattt gtccaagact gaacgagcag gttcgttgcg gtttgaactc tgtagttctg
    13381 ccccacaacg gacggccaac caaccagttc gtcatcacca gccaccagaa actccgcaat
    13441 ctgggtttgt gaccgagatt cagggaggat gccagaaagg ataatcgaca cggcttgagc
    13501 cgagtttgtt ccggtatcat caacaagcgt ccccttgtgt gtaatccgca ccgtatagac
    13561 ggcctctgct gggggattgg ttacaacgac ctgttcggtg ttgtcccgga cattgtcccc
    13621 agtggtagct gcgtatgatg ggttcgcagg atcgagcacc caagggaagt tggtcacacc
    13681 tgatgaactg atgatgcgga ggtcgagatc gttcaccagg acgctgttgg tcggatcaag
    13741 actttccgct ggtgaagtcc cagccgggtc cgtccaacca atgctgactg taacgggaac
    13801 accacctgtg gcaatcacgg gaaattcgat atagtctcca tcattcagca ggacttcttt
    13861 gatgtactgc tttccgccgt cgtcgtaatc ctgctgaatg agcaatgccg ctttctccgc
    13921 attgatgaga ccccaacctg tcttgtaatc aggtccggtt gtttcggcct catcagcagt
    13981 atgagtgatg attgtcttga gtgtggatgc ccagtacgga agctccggtg aaagttgttc
    14041 tcgcaaatca accagcagac cgagtgctcc tgctgcggat ggcgaggaga aactggttcc
    14101 tgtcgcatat gacgggttgt ttgtgtcacc cgtcgataac acgtagtagt aggggttgtt
    14161 cgggtcggca ggatttctca ccggagtcgt aaggtctacg cctgcggcga ctacgtcagg
    14221 cttaattcgc ccgtcatcag tcggcccgaa gcaactgaag gcagcaatgg ataccgatga
    14281 tgtacccccg taccctcccg aaatcttgta cactgcgccg actgtaagaa cgttcttggc
    14341 aactccatga tctgaaaccg tgtcatatcc gttctgatag tagtcgttag gacgtgacgt
    14401 attgctccag atcggtactg tgttcgaaaa ggtgatgtac catcttccat ctgttggcgc
    14461 cgcatcgtcg cggtcattcc cagctgccca aacgggaaga ctgtatgggt tgccgtaggc
    14521 gagcgcgtcc gtattcgtta catccgaatc gtactcgccg aagtagtggc tctcattggg
    14581 acttatggca atgtcgcccc accaacagtt tgtataaacg ggagtagggg tgtagacagt
    14641 tatgaaaccc caccccgctt gctcgccata ggagtgattg gagacgtgga gatcattggc
    14701 gtatgcggag ttcatctccg cttcgatgtc attgacccaa tcgtaagtcc agagctgagc
    14761 ctgatacgcc atgccccttg ggtcgcctga atatgcgaca ccagcagctg tcatcgttcc
    14821 agcgactgcg gtaggatgta gatcaacagg caacgagctt gttccgtctt gatcaacggc
    14881 gcgagatgtt gcaccgcctg atgtgaattc gtagtggttt gtgagaacat ctccgccgtc
    14941 ccaaatcccg aggagtgtat tggccccgga cacatcaaga ccgctgccgc cacccggcca
    15001 cacttcatct acggagatgg tgtctgctgc tcctgcgttg aatgtcacat tgtacttggg
    15061 aatcccgttc cgtataccaa cgaggcccat gacgccacct tcgggcagac gtgcgctttc
    15121 ggcgaatccg tgttgttctg cccactcgcg agcttgctcc atttcctgct gatgagcttt
    15181 cagtagctga gacgatttcg cccaaaggcg ctcgcgacgc ttcatctcag catctgcaat
    15241 cgaactcggt tggtcatccg ccgtacgaac gcctttgttc agccattgcc gcattcgttc
    15301 ctgcttggtc ggttcgtcgg gttccgcgac tgagtcggca ctggtaaggg cgtttgcttc
    15361 ggtttcctgc tttgccttca gccatgcctc gtatgtggga atttccatct cgggcatgtc
    15421 cgcttctcgt tgcacttcgt tgaagtgctc gatggaagcg atctccgccc tatactcatc
    15481 ggtgcggtct gtactctgcg cctgcgcggt ggtcatacct acgaatacgg aagcggccag
    15541 aaccaaccgt gcgacgccag cgatcattct tgtctgcatt gtcttctccc ttcgggttga
    15601 ggatacaccc acgagcagcg cggatgcatc catattcttt ctcttctcgc taacgtcagt
    15661 ggtgaggggc gcgagtttac gagcgtacct ctccaccacc ttgttcggct tcatattatt
    15721 tcttcccctg aagtagccat acagctgggg cagccattag caggaatacg gggaaaaata
    15781 cgacaatatt gaaccatagg atatgcatct ttgcgtttcc tgttaagggg atcgtgatgc
    15841 ccccgaggga acgctctaaa tcagagtaac cttctatgcc gtatattgac gttaatattc
    15901 cgccaatata cgccgtgacg gcacaggcta taaacatcca gatggccagg accgcggtgg
    15961 caagcaatgg tcgaattaat ttcatttctg tagccgaatc gcggcgctca ccaactttgg
    16021 ctcgagccta gcaggcgcag cct
//
LOCUS       DAOQWR010000050        15914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4110_length_15914_cov_5.509868, whole genome shotgun sequence.
ACCESSION   DAOQWR010000050 DAOQWR010000000
VERSION     DAOQWR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4110_length_15914_cov_5.509868"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1002)
                     /locus_tag="PKK36_05680"
     CDS             complement(223..1002)
                     /locus_tag="PKK36_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR94085.1"
                     /translation="MPDPENRLLEIDALHVRYKKIHAVDGASVNIEPGECVALVGESG
                     CGKTSLCRAVLGLERISAGRVLFAGSSLNEFDAGDWKKYRNRVQMIFQDPGESLNPRL
                     TAGGMLSEVLKVRRGLPAPARQAETERLLQTVGLDPALAGRYPHELSGGQKQRLGIAR
                     ALAVNPDLIIADEPVSALDVSVQVQILNLLLDIQTARGLALLLIAHDLAVVRYMCRRV
                     YVMNRGQIVESGMAARIFSRPQHPFTRALLDAVPDVDRCRG"
     gene            complement(995..1960)
                     /locus_tag="PKK36_05685"
     CDS             complement(995..1960)
                     /locus_tag="PKK36_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94086.1"
                     /translation="MSETRPPMEPLVRVEDLCIDFGPVRVVDGLSFRIDAGEIVALVG
                     ESGCGKSVSALALARLVHELSGRIWFDGRDILQMSGRELCDLRGGEIAYIFQEPGSSL
                     NPVVRVGDQISEALRLHCYRGNMRDEVIRLLDSVGIPEPEQRRRAFPHQMSGGQQQRV
                     MIAMALACRPRLLVADEPTTALDVTVQVQILDLLQKIQRESGTAILLITHNLGLVAGR
                     ADRIYVMYAGRVAEAGPVASVLRSPRHPYTRALLKAVPRLNSTGGRLPGIPGSVPPAD
                     AMPPGCRFHPRCPLADRRCAEVPPPAETIEGEHCAACYHWNKSDA"
     gene            complement(1953..3248)
                     /locus_tag="PKK36_05690"
     CDS             complement(1953..3248)
                     /locus_tag="PKK36_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94087.1"
                     /translation="MSERPTIIEKYDRDGLIRHSLIVMIFTHAGSAANMVFHMIMGRA
                     LPKVEYGIMSSMLGLGLILATPMVALATTLAHYTAQLSATGNAGAARQLMRYWLKRLT
                     PFSLAFLALGFLFSGLLAEYFNLQTRTPVLITALIFSALIYSPVFPGILQGLQSFVWM
                     SLAYNAWGLVRLLLGTALVLMFGGLAVYPLAAQAVGYALGLIFGWWLIRRELRNEPEA
                     PTVIHGTDSYFLRSLVVLTGFAFLMNADIVMVKHYFAPEAAGLFAQVATIGRIMVFLV
                     QPVAGAMFPKVISGGEMSRAHAVTFLRAIAFSGLITLAAVAGCLLLPWLPLRIIFGIR
                     TPSAEQILLLRTVACAMAPLGITFLLLNYELAQRRFGPAWPLVGCAILYAAGVHRWHG
                     HVLHVAWVLGGVSLLSLVLITAGIAIQMFRAVRREGSRV"
     gene            complement(3258..4028)
                     /locus_tag="PKK36_05695"
     CDS             complement(3258..4028)
                     /locus_tag="PKK36_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR94088.1"
                     /translation="MKLSIVVPAYNEERRIRPMLDAYLEFFTPRYGRDFEVLVVVNGS
                     TDRTADVVREYAARFPQLRCIVESEKIGKGGAVKLGFESARGEIAGFADADGSTPPES
                     FQALVDEIGPAADAVIASRWFRESEVYPRQTLARQIASRVFNGFVRLMLGLKIRDTQC
                     GAKVFRKEALDCVLPSLGLTRWAFDVDILFQLHRAGFTIREAPTKWHDVGGSQLRIFR
                     ASVQMFVAVTRLRLLYSPFCWVVALYDRTLGRIVKLKV"
     gene            complement(4025..5617)
                     /locus_tag="PKK36_05700"
     CDS             complement(4025..5617)
                     /locus_tag="PKK36_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94089.1"
                     /translation="MRENASIRLVFVLALTVCVLLTAANILLGELNQDEGWYLYAAGQ
                     VARGALPYRDFAFTQAPVMPFVYAAADVLVRAWGVAGGRIFTAFLGFLSVLAASVLAA
                     KVASRRWRAHAALTAFVLVGINVYQSYFTAVVKTYSLCALLLTTGMLVLAAGYNSRRL
                     LSCFISGILLALAAGTRISTGIALPVVFLFLLFWGWRADWRSWFAFGLGGGLGLLAVF
                     LPLFLAAPEGFIFGVLQYHTLRAPGSIVYQMVLKAGFLSRWVQAYMIASVLAAALIVL
                     RLFPFRRPPVTDSPVGVLVLLAVFGAISLVHFLAPFPYDDYQVPVYPLLGAALAAAAV
                     NMLAGLTPEEGDFRRRAMGALLWTVFSVSVLSSFSSPINQQWMILGRDRIWWLTKDKP
                     DLIRLREAGRWIRENSSPGDLLLTQDTYLAVEAGRQVPHGLELGPFSYYPDWDSARAE
                     KLKVLNRESMLNLLEKTRAPVAAFSGYGLSIRSPEVEPLSREEQALLLIALDTRYETA
                     RIMEHFGQAHTTLQLMTRRSTP"
     gene            complement(5621..6799)
                     /locus_tag="PKK36_05705"
     CDS             complement(5621..6799)
                     /locus_tag="PKK36_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNR94090.1"
                     /translation="MMEQSVEELLAKLVAVPSVNPEHSADPAVTGERRVADFIAGYLS
                     DRGFQIEWLEKTPGRPNLIASYGPDDPKYTVLLEAHLDTVGIDGMTVAPFDPQIRDGR
                     MYGRGTTDTKGPLAAALWALDRDRLQALSGASCRVIFIGAMGEETGNEGAFEVAEHGI
                     RADEALILEPTEMQPIHTHKGTLWFRVELLGLSAHGSNPELGRSAILAMKKVIDALNS
                     QIEADQHRFEDPVLGLPTLNIGRIHGGTALNIVPEKCVMDVDRRTLPAEDTRAILRRV
                     DVVLAKLKSSGEIREGKVHLIKDCPAVATPPDGRLIGRLVKCCAETGVKAGAAGAAWY
                     SDAGAFSAVCGETAVFGPGSIKQAHTCDEFIEISELHRGAKCLRGFFSALADDVKGMR
                     "
     gene            6999..10562
                     /gene="smc"
                     /locus_tag="PKK36_05710"
     CDS             6999..10562
                     /gene="smc"
                     /locus_tag="PKK36_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNR94091.1"
                     /translation="MYLKSIELVGFKSFAEKTKLEFEPGMTAIVGPNGCGKSNVSDAI
                     RWVLGEQSAKAMRGANMQDVIFNGTDAHKPMGMAEVSLTLTDCESSLGTEFNEVTVTR
                     RIHRSGEGQYFINQAPCRLKDIQRLFMDTGIGTNAYSLMEQGRIDRILSSRPEDRRIV
                     FEEASGITKYKTDKKEAIRKLEHTENNLIRLTDIIKEVKRQIISLQRQAGKAKRYKTL
                     QEQLRRLDIFVTRNRLVDLETSVELLKKSLAAVTRQEDEARASVAKTEHDADKIRDEL
                     MAIEGRISQAMDEASEARADLDRTQQMIAVNKDRIAELESLSQRDSRDAEESRKQLEI
                     HRASLSALEQQMEKAEAARSAAEQELEKQNAKLDRLNQDLENKRHQVNKLRDDLVDSE
                     SRHAHLANELSSLEAKERSSVIKRERLFTEQSDLERTVQSFMDRQKKMAERLTALQAD
                     VVQHQGLLDDLLHKREARTAESRTLQQKIHELQRELATQKAQIHLLNSREAEEEAFPA
                     GARVLLGNSGELPVKPAGILGALADAVEAEGEYRTALEAVLRAWMDAVIVSDSGTARQ
                     ILEALREKKSGPVRMLSAGGHPAEEPPVSGDLLIRHVKCEDNIRPLLGRMLRNAIVVN
                     HLHDIPADIPDGTIYVTRDGILLRSDGSAEYWMPGASTASPLARKHMLASCREECGKL
                     EKQIGMREDAQSKLQSDDRSAENTIAEAQQTLETCRRNLALCEGENQVIAAETRQARE
                     RFETVEYEIRQLSEQEGAGGSRRSEILKELEELREYQAKTRAAIASGTDELRVLEQNR
                     SDFMQEVTECRVRFAEQRQEVSHLLSRKEPLQAQMASLEALISDRANGISSYQMRIED
                     LKKASQEGAAQIPPLEEAVKARNEELEVLRGQRGEKSALRTQWEQKIHELRAGMDDVH
                     EKRSKIEVQLAQQEMRRQNSLDRILSEYRISRQQLEEEPAPEWGEETQPEAEALETTI
                     AEIRAKIESMGPVNLVAIEEHEELEQRYTFLTQQQDDLVKAKQQLMDMIRKINQTTTE
                     MFSQTFDQVNRNFQDMFMKMFGGGSAKLVLVDEEDVLESGIEIIARPPGKKLQTVSLL
                     SGGERTLTAVALLFALYMVKPSPFCVLDELDAALDDTNIGRFVTTVQGFLEKSQFIVI
                     THNRQTISAASVLYGVTMEKHGISKIVSVKFSDRTAEEAAG"
     gene            complement(10654..11802)
                     /gene="holA"
                     /locus_tag="PKK36_05715"
     CDS             complement(10654..11802)
                     /gene="holA"
                     /locus_tag="PKK36_05715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNR94092.1"
                     /translation="MSSKRKSEQDGGRKPVYLVLGGDEFAVSAKARAIVDELCPPAEQ
                     AFGLERIDGSPSKADEAVETIRKCMDAVMTVGFLGAGKTVWLENASFLSEGDPGRIER
                     VKHELERLTDLIKNGLPPGQHLVVSSPAVSRRMAFYKACEKAGEVHAFEIESRPWQAV
                     KDAQGRLAELLREKNLKMSQAVAEAFLERTGYDTRQMAMEIEKLAVSCGEGASVTADH
                     VRLLVSPGREAPVFELTEHVGMRNLPAALKSLKNLQAQKLNAMAIIYPLQSHIRMLAL
                     IRDCLNRRWCRAKAGYGESPWSENPEADAVLSRLGKDDPRKMHSFRVQKLAEQCAYWT
                     DRELARALRQIVKTHERLVSSPVSSDLLIELLIVALCAKSGKRGAVSG"
     gene            complement(11799..12659)
                     /locus_tag="PKK36_05720"
     CDS             complement(11799..12659)
                     /locus_tag="PKK36_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94093.1"
                     /translation="MGKVRHILKMLLLLLAAAGAGAGLTLWALHALGADTDGMSRLGR
                     GVLKAGGNEWKIAATEHFTGYAPSAELVELLLRKAEHASREVEIALDIPPLTNHAHCF
                     LITDSNLWKTVRQRGGWRTDGRALQYGNEFVLFIPDAETEMEDSIPHEIVHFRLRQVY
                     ANREPLALEEGLASYLGWEITLEWHLGGGKIITRVQPRLPGETLWSLNELVSAQAYDQ
                     NADRNRAFYRQSEEWVRGIVSLIGPESLGEFVRLVFDGEMTWDRALREKWGLSDLQMD
                     KLSAIAESGK"
     gene            12785..13237
                     /locus_tag="PKK36_05725"
     CDS             12785..13237
                     /locus_tag="PKK36_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNR94094.1"
                     /translation="MKYILGARETGCFLCAMLPDGNDSENLILKRGRTCFIVMNRYPY
                     NNGHLMVCPLRHVDSLGAMTAKERAEMMELTSECVALLQKVMKPDGFNVGINLGAAAG
                     AGLKDHIHIHIVPRWNGDTNFMPVLGQTKVIPQALHELRDELAAAMQA"
     gene            13263..15755
                     /locus_tag="PKK36_05730"
     CDS             13263..15755
                     /locus_tag="PKK36_05730"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNR94095.1"
                     /translation="MKYKLLLTAWLYSLALPAVSATHLDEAKLDALQRQAFDYFLDCR
                     ATNGLVPLRLGDPRLTSPQAEGLHLSALIAGTGRGWLKRPEAARLAAQTLSTCARLPR
                     IHGFFRESYSIHTLEPLPAQSRADFMGTAALIAGALTCEQFFDRETPTENTIRETARQ
                     LYAEAEWNWMLRDNEGESGCTPALYWSEDGGYSDERADGSGSLQGMLACLLAAGAPAH
                     SIPAACWNKGWARGYQWEHISGADLVVSPPLFSQIYPQIWLDLKNRRDRHTDYWRNAF
                     HAVRLNYRYCTSRLYPGTALWGLSTCEGPKGIDDYGCPPLQGHVDEDAVLAPAAAISA
                     IGWAPSEALAMVEEMETNHREAVWGKYGPRASFSPRHNWVSRDYLATDLGAMVCMIEN
                     HRSGLVRDLFSSHDAVRRALADTGFTGVIADFEEASGMEPYLTWESSSTAMQKIAADI
                     SREGFQSLEVVCRDRTGMLAGRSALRDFQPFRYLTLWLSSGSELDIALEDSGGRSVQL
                     AQESAKGAEDGWTRHVYSIEGGECDLTDVRRILFTFHPQRPGDPLWMDGIFLAAQKPG
                     PRPEGIQNLRAESSRMPGEAVLRWDPPADDVFTCHARYSARPIRDYESFQKATAVSTA
                     PFRHADPARAEIHVPNLVPGETYHFAVQTETLDHEKSENTAVTSLTLPRTKAPDEFLV
                     DHFDSGGILNWRPAGTGTEVAADTGNALLGSGGSLRIGVSDAKKASKASAYADTDFRD
                     FSQYGYLALWVSGKTAIQIALANEKGDTEYLPEQKTALADGWSPVFFDLAGIRKLDRK
                     TISRILIHVRPDTESRDRGIWVDSLRLTKTRN"
BASE COUNT         3588 a         4885 c         4703 g         2738 t
ORIGIN      
        1 gcgatgccca aaccgggcag gatgcgcccg aacacaatgc tgtccggcat cttgaacaca
       61 aacttgcaga ccccgctgag aataatcata ttggcaagat tgtctgcgaa cagcgcccaa
      121 aacgcattcc actcgccctt ggaaaagatt cttcctccca tctcatttct ccttgtctat
      181 gccatcgttc cgcagttttt tacacccgtg cccacgacat cgtcaacccc tgcatcggtc
      241 cacatctggg acagcatcca gcagggccct cgtgaagggg tgctgcgggc ggctgaaaat
      301 ccgggcagcc atgccggatt caacaatctg cccgcggttc atgacgtaaa cgcgcctgca
      361 catgtatcgg accacagcga gatcatgcgc aatcagcagc agggccagcc cgcgcgcggt
      421 ctgaatatcc agaagcaggt tgagaatctg aacctgcacg gatacatcaa gggcggaaac
      481 cggttcatcg gcaatgatca gatcggggtt caccgccagt gcgcgcgcga tacccagacg
      541 ctgcttctgg ccgccgctca gttcgtgcgg ataacggccg gccagcgccg gatcaagtcc
      601 taccgtttgc agaaggcgct ccgtttcggc ctgacgggcg ggtgccggga gaccccggcg
      661 caccttcagc acttcactga gcatcccgcc cgcggtaaga cgcggattca gagactctcc
      721 cggatcctga aagatcatct gcacccgatt gcgatatttt ttccagtcac cggcatcaaa
      781 ttcattgagg ctgctcccgg caaaaagcac ccgcccggcg ctgattcttt ccaggcccag
      841 gaccgcccgg cagagagagg ttttcccgca tccgctctcg cccaccagcg cgacacactc
      901 gcccggttca atgttcacgg aagccccgtc aacggcatga atctttttgt agcgcacatg
      961 gagcgcatca atttcaagaa gtctgttttc cggatcaggc atcggattta ttccagtgat
     1021 agcaggcggc acaatgctcc ccctcgatgg tttcagcagg cggcggcact tcagcacacc
     1081 gccggtccgc cagcgggcat cgaggatgga agcggcagcc cgggggcatg gcatcggccg
     1141 ggggaacgct tcccggaatg ccgggcagcc ggccccctgt agaattcagc cgcggcacag
     1201 ccttcagcag cgcccgcgta taggggtggc gaggagaacg cagcaccgag gctacaggtc
     1261 cggcttcggc cacgcggccg gcatacatca catagattct gtccgcacgg ccggcaacaa
     1321 gtccaagatt atgggtgatg agcagaatgg ccgtgccgga ttcgcgctga atcttctgca
     1381 ggagatcgag tatctggacc tgcacggtca cgtccagcgc cgtggtgggt tcatccgcca
     1441 ccagcagccg gggccggcag gccagggcca tggctatcat cacgcgctgc tgctgtccgc
     1501 cgctcatctg atgcgggaaa gcgcggcggc gctgttccgg ttccggaatg ccgacagaat
     1561 ccagcaggcg gatgacctca tcgcgcatat ttccacgata acagtgcagg cgcagggctt
     1621 ccgaaatctg atcacccact cgtacaaccg gattcagcga agacccgggc tcctggaaaa
     1681 tataggcgat ctcgccgccc cggagatcac aaagttctct tccgctcatt tgaagaatat
     1741 cgcggccgtc aaaccaaatg cggccggaga gctcatgaac aaggcgcgcg agcgcgaggg
     1801 ctgacacgga cttcccgcat ccactctcgc ccaccagcgc cacgatttcg ccggcatcaa
     1861 tgcggaatga aagaccatcc accacccgca cgggcccaaa atcaatgcaa aggtcctcga
     1921 cccgcaccag cggttccatc ggcgggcggg tttcagacac gggagccctc ccgacgaacg
     1981 gcacggaaca tttgaatggc gatgcccgca gtgatcagaa caagcgaaag cagactgact
     2041 ccgccaagca cccacgcgac gtggagcaca tggccgtgcc agcggtgcac accggcggca
     2101 tacaggatcg cgcagccgac cagcggccat gccggcccga aacggcgctg cgccagctca
     2161 tagttcagga gaaggaaagt aatccccagc ggcgccatcg cacatgccac cgtgcggagc
     2221 agaagtatct gttccgcgga cggggtgcgg atgccgaata tgattctcag cgggagccac
     2281 ggaagaagca ggcagcccgc aacggccgcg agggtgatca gcccggaaaa ggcaatcgcc
     2341 cggaggaagg tgaccgcatg cgcgcgcgac atctctcccc ccgagatcac cttggggaac
     2401 atcgcccctg cgacaggctg aaccagaaac accataatgc gcccgatggt cgcaacctga
     2461 gcaaaaagtc ccgcggcttc cggcgcgaag tagtgcttca ccatgacgat gtccgcattc
     2521 atcagaaatg cgaaacccgt cagcacgaca agcgagcgca ggaaataact gtccgtgccg
     2581 tgaataaccg tcggcgcttc cggctcattc cgcagctcgc gccggatcag ccaccagccg
     2641 aaaatcagcc cgagagcata accgacggcc tgcgcggcca gcggataaac agccagcccg
     2701 ccaaacatca gcacaagggc agtaccgagc aaaagccgga ccaggcccca tgcattatag
     2761 gcgagactca tccagacaaa ggactgcagt ccctggagaa tgccggggaa aacaggagaa
     2821 tatatcagcg cggaaaaaat cagcgcggtg atcagcacgg gcgtccgggt ctgcaggttg
     2881 aagtactccg caagcaagcc gctgaacaga aaaccgaggg ccagaaaggc cagagaaaaa
     2941 ggagtcaggc gcttgagcca gtaccgcatg agctgacggg cggcgcccgc atttccagtg
     3001 gcgctcagct gtgcggtata gtgcgccagc gtggtggcca gcgccaccat cggcgtggcc
     3061 agaatcaatc ccagccccag catggaggac atgatgccat actcgacctt gggcagcgcg
     3121 cggcccatga tcatgtgaaa aaccatgttg gcggcgctgc ccgcatgcgt aaagatcatc
     3181 acaatcaggc tgtgacgaat cagcccgtcg cggtcgtatt tttcaatgat ggtcggacgt
     3241 tcactcatgg acctgcttca caccttcagt ttgacaattc ggccgagggt gcggtcatac
     3301 agcgcgacca cccagcaaaa gggggaatat aagagccgca agcgcgttac ggcgacaaac
     3361 atctgaacgg aagcccggaa aatgcgaagc tgtgaaccgc cgacatcgtg ccatttcgtg
     3421 ggagcctcgc ggatggtaaa gcccgcgcgg tgaagctgaa aaagaatgtc cacatcgaac
     3481 gcccagcgcg tgagtcccag cgagggcagc acgcaatcca gcgcttcttt cctgaatacc
     3541 tttgcgccgc actgggtgtc gcgaattttc aggcccagca tcaggcggac aaagccgttg
     3601 aacacacgag atgcaatctg ccgggcaagc gtctgacggg gatacacttc cgactccctg
     3661 aaccagcgcg aggcgatgac cgcgtcagcg gccggcccga tttcatcaac cagcgcctgg
     3721 aaagattccg ggggagtgga tccatccgca tcggcaaatc cggcaatttc tccgcgggca
     3781 gattcaaacc cgagtttcac cgcaccgcct ttgccgatct tctcggattc gacgatgcag
     3841 cggagctggg gaaatcgggc ggcatattcc cgaaccacat cggccgtcct atccgtggat
     3901 ccgtttacga ccacgagcac ttcgaaatca cggccgtaac gcggcgtgaa aaattccaga
     3961 taggcgtcga gcatcgggcg aatgcggcgt tcctcgttgt acgccggaac cacaatactc
     4021 agtttcatgg agttgacctc cgcgtcatga gctgcagcgt ggtgtgcgcc tggccgaaat
     4081 gttccatgat gcgggcagtc tcgtaccgcg tatcgagcgc gatcagcaga agggcctgct
     4141 cctcccggga aagaggctcc acctccggag accggatgga aaggccataa ccgctgaagg
     4201 ccgccaccgg cgcacgggtt ttttcgagca ggttcagcat gctttcgcgg ttcagcacct
     4261 tgagcttctc tgcccgcgcg gagtcccagt ccgggtaata gctgaacggg cccagctcga
     4321 gcccgtgcgg aacctgacga ccggcttcaa cggccaaata ggtgtcctgc gtcaggagca
     4381 ggtcgccggg cgatgagttt tcgcgaatcc agcggccggc ctcacggagc ctgatgagat
     4441 caggcttgtc tttcgtaagc caccagatgc ggtcacgccc aagaatcatc cactgctggt
     4501 tgatcgggga tgaaaacgag gaaagcaccg agaccgagaa caccgtccac agcagggctc
     4561 ccatcgcgcg gcggcggaaa tccccctctt ctggggtcag gcccgccagc atgttcacgg
     4621 ctgccgccgc cagcgccgcg ccgagcagcg gataaacagg cacctgataa tcatcataag
     4681 gaaacggcgc caggaaatgc acaagcgaaa ttgcgccaaa caccgccagc aggaccaaaa
     4741 cacccaccgg actgtccgtc accggcggac ggcggaatgg gaacaggcgc agtacaatca
     4801 gtgcagcggc cagaaccgac gcgatcatgt acgcctgcac ccagcgcgag agaaatccgg
     4861 ccttaagcac catttggtag acaatcgaac caggcgcgcg cagtgtgtga tattgaagca
     4921 ctccgaaaat gaaaccttcc ggggccgcca ggaacagggg caggaacacc gccagaaggc
     4981 cgagcccgcc gccgaggcca aatgcaaacc aggaacgcca gtccgcccgc cagccccaga
     5041 aaaggagaaa caggaaaaca accggaagag caatgccggt tgaaatccgg gtgccggcgg
     5101 ccagcgccag cagaatcccg gaaataaaac aggacagaag cctgcggctg ttgtatccgg
     5161 cggccagcac cagcatgccg gtcgtcagca aaagagcaca gagggaatag gttttgacaa
     5221 cggcggtaaa ataactctgg tatacattga tcccgaccag aacaaaggcg gtcagggcag
     5281 catgggcacg ccagcggcgg ctcgcgacct tcgcggccag aactgaagcg gccagcacac
     5341 tgagaaaacc gagaaatgcg gtgaatatcc ggccgccggc gaccccccaa gcccgcacca
     5401 gaacatccgc ggccgcatac acgaaaggca tcaccggcgc ctgcgtaaag gcgaaatccc
     5461 ggtacgggag tgctccgcgc gcgacctgcc cggcggcata aagataccag ccctcgtcct
     5521 ggttcagctc accaagaaga atgtttgccg ctgtcagcag gacacaaacc gtcagcgcaa
     5581 ggacaaaaac aagccggatt gaagcattct cccgcatggg ttaccgcatt cctttcacat
     5641 catccgccag tgccgaaaag aaaccgcgca ggcatttcgc gccgcggtgc agttcgctga
     5701 tctcaatgaa ctcgtcgcac gtatgggcct gtttgatgct cccgggcccg aacaccgcgg
     5761 tttcgccgca caccgccgaa aaagctccgg catcactgta ccacgcggcg ccggccgcgc
     5821 cggccttcac gccggtctct gcacagcact ttaccagcct gccgatcagc cgaccgtccg
     5881 gaggcgttgc cacggcgggg cagtctttga tcagatgaac cttcccctcc cggatttctc
     5941 cgctcgactt cagcttggcc agcaccacat ccacacgccg cagaatagca cgtgtatcct
     6001 cggcgggtaa agtgcgccgg tccacatcca tgacacactt ttcggggaca atgttcaatg
     6061 cagttccacc atgaatccgg ccgatgttga gcgtgggcag ccccaaaacc gggtcttcaa
     6121 aacgatgctg atcggcctca atctggctgt tcaacgcgtc tatgaccttt ttcatggcca
     6181 gaatggcact ccggccgagt tcgggattcg agccgtgggc ggacagaccc agcagttcaa
     6241 cgcgaaacca cagcgtcccc ttgtgcgtat ggatcggctg catctcagtc ggctccagaa
     6301 tcagggcctc atccgcacgg atgccgtgtt cggccacctc aaaggccccc tcattgccgg
     6361 tttcctcccc catggcgccg ataaagatca ctcggcagga cgcgccggac agcgcctgca
     6421 gccggtcgcg gtccagcgcc cagagcgcgg cggccagcgg ccccttggtg tcagtggtcc
     6481 ctcggccata catcctcccg tcgcgaattt gcggatcaaa gggcgccacg gtcatgccgt
     6541 ctatgcccac cgtatccaga tgagcctcca gaagcaccgt gtatttcggg tcatccggac
     6601 catatgaagc gatcaggttt ggacggcctg gagtcttctc cagccattcg atctggaagc
     6661 cccggtcgct cagatatccg gcaatgaaat cggccacgcg ccgttcgccg gtcacggccg
     6721 ggtccgcgct gtgctccgga ttcacactgg gaacagccac cagtttcgcc agcaattctt
     6781 caacgctttg ttccatcata aatcaactcc gccccggaac cgcaaacccg cgcaagaaaa
     6841 cacttgaatt cggctcaaaa aaagggcaca aggcactgtt tttttgtcgc caatatttcg
     6901 atttcttccg gatattgcga tcaaattctt atttaagttt gcggattgcc gggattggaa
     6961 tcgggaccgg ccgacacatt tgaagcgagg gtcaggcatt gtatctcaaa agtattgagc
     7021 tggtcggttt taaaagtttt gcggaaaaaa ccaagctgga attcgaaccg ggcatgaccg
     7081 ccattgtcgg gcccaacggc tgcggcaaga gcaatgtatc cgacgcgatc cgctgggtcc
     7141 tgggcgagca gagcgccaag gccatgcgcg gcgccaacat gcaggacgtg attttcaacg
     7201 gcaccgacgc gcacaagccc atgggcatgg cggaggtcag cctgaccctc accgactgcg
     7261 aatcatcgct gggcaccgag ttcaacgagg tcaccgtcac ccgccgcatc caccgttcgg
     7321 gcgaaggcca gtatttcatc aatcaggccc cctgccgcct gaaagacatc cagcggctgt
     7381 tcatggacac cggcatcggg accaatgcct attcactgat ggaacagggc cgcattgacc
     7441 ggattctcag ttcgcgtccg gaggaccgcc gcatcgtgtt tgaagaggcc agcggcatca
     7501 ccaaatacaa aacggacaag aaagaggcga tccgcaagct ggagcacacc gagaacaacc
     7561 tgatccgcct cacggatatc atcaaggaag tgaagcgcca gatcatctcg ctgcagcggc
     7621 aggcgggcaa ggcgaagcgc tacaagacgc ttcaggaaca gctgcggcgg ctggacattt
     7681 tcgtcacccg caaccggctg gttgatctgg aaaccagcgt ggaactgctg aaaaagagcc
     7741 tcgccgccgt gacccgacag gaggatgaag cgcgcgcatc ggttgcaaaa accgagcacg
     7801 atgccgacaa gatccgcgac gagctgatgg ccatcgaggg ccgcatctca caggccatgg
     7861 acgaagcctc ggaggcccgg gcggatctcg accgcacgca gcagatgatt gcggtcaaca
     7921 aggaccgcat cgcggagctg gagagtcttt cccagcgcga ctcgcgcgat gccgaggaat
     7981 cccgcaagca gctggaaatc caccgcgcgt cgctttcggc gctcgaacag cagatggaga
     8041 aggcggaggc cgcccggagc gcggccgaac aggagctgga gaagcagaac gcaaaactcg
     8101 accggctgaa ccaggacctg gaaaacaaac gccatcaggt gaacaagctt cgcgatgatc
     8161 tcgtggattc cgagagccgg catgcgcacc tggccaatga gctttcctcc cttgaagcaa
     8221 aagagcgcag ttcggtgatc aaacgcgagc gccttttcac cgagcagagc gatctggagc
     8281 gtacagtgca gtcgttcatg gaccgtcaga agaaaatggc cgagcgactg acggcccttc
     8341 aggcggacgt ggtccagcac cagggccttc tggatgatct gctgcacaag cgggaggcgc
     8401 gcaccgcgga aagccggaca ctgcagcaga agatacatga gctgcagcgc gagctggcca
     8461 cacagaaggc gcagatccac ctgctgaatt cccgtgaagc ggaagaagag gcttttccgg
     8521 caggcgcaag agtgctgctg ggcaacagcg gagaactgcc cgtgaagccg gccggcattc
     8581 ttggcgcact ggccgatgcc gtcgaggcgg agggcgaata ccgcacggcc cttgaagccg
     8641 ttctgcgggc atggatggat gccgtgattg tatctgacag cggcacggcc cgtcagattc
     8701 tggaagccct gcgcgagaag aagagcggcc ctgtccgcat gctgagcgcg ggaggccatc
     8761 ctgcagaaga gccgccggtt tccggcgacc tgctgatccg ccatgtgaaa tgcgaagaca
     8821 atatccggcc cctgctgggg cgcatgctca ggaacgccat tgtggtgaac catctgcacg
     8881 atatcccggc ggacataccg gacggaacaa tctatgtgac acgagacggg attctgcttc
     8941 gaagcgatgg atccgccgaa tactggatgc cgggcgcctc cacggcgagc cccctcgccc
     9001 gcaaacacat gctggcctct tgccgcgaag aatgcggcaa gctggaaaaa cagatcggga
     9061 tgcgcgagga tgcgcagagc aaactgcaaa gcgatgaccg gtcagcggaa aacaccattg
     9121 ccgaagcaca gcagaccctt gaaacctgcc gccggaacct ggccctgtgc gaaggggaaa
     9181 accaggttat cgccgccgag acccggcagg ccagggaacg gttcgaaacc gtggaatacg
     9241 agatcaggca gctttccgaa caggagggcg cgggcggatc gcgtcggtca gaaatcctga
     9301 aagagcttga agagctgcgg gaataccagg ccaaaacccg tgcggccatc gcatccggca
     9361 cggatgaact gcgcgtgctg gagcaaaacc ggagcgactt catgcaggag gtcaccgaat
     9421 gccgtgtgcg tttcgcagag cagcggcagg aggtcagcca tctcctcagc cgcaaggagc
     9481 cgctgcaggc gcagatggcc agcctggaag cgctcatcag cgaccgcgcc aacggaatca
     9541 gctcgtacca gatgcggatt gaagacctga agaaagccag ccaggaggga gccgcgcaga
     9601 ttccgcccct ggaggaggcc gtcaaggcgc gcaatgaaga actggaagtg cttcgcggac
     9661 agcgcggaga gaagagcgcg ctgcggacgc agtgggagca gaaaatacac gagctgcgcg
     9721 cgggcatgga tgatgtccac gagaagcgga gcaaaattga ggtgcagctc gcgcagcagg
     9781 aaatgcgcag gcagaattca ctggaccgca ttctttccga ataccgtatt tcccgccagc
     9841 agctggaaga ggaaccggcg ccggaatggg gagaagaaac tcagccggag gccgaggcgc
     9901 tggaaaccac catcgcggaa atcagggcta aaatcgaatc catgggaccg gtgaacctgg
     9961 tggccatcga ggagcacgag gaactggagc agcgctacac gttcctgacc cagcagcagg
    10021 acgacctcgt caaagccaag cagcagctca tggacatgat ccgcaagatc aaccagacca
    10081 ccaccgagat gttttcgcag accttcgacc aggtgaaccg gaacttccag gacatgttca
    10141 tgaaaatgtt cggcggcggt tccgccaagc tggtgctggt ggacgaggag gacgtgctgg
    10201 aatccggcat cgagatcatc gcccgcccgc cgggcaagaa gcttcagacc gtatcgctgc
    10261 tttccggcgg cgagcgcacg ctgacggcgg tggcgctgct gtttgccctc tacatggtca
    10321 agcccagccc gttctgcgtg ctggacgagc tggacgccgc gctggacgac accaacattg
    10381 ggcgcttcgt caccacagtg cagggattcc ttgaaaaatc gcagttcatc gtcatcacgc
    10441 acaaccgcca gacgatcagc gcggccagtg tgctctatgg cgtgacgatg gaaaagcacg
    10501 gcatctcaaa aatcgtctcc gtcaaattct cggaccgcac ggccgaagaa gccgccggct
    10561 gaaaagttcc aatcattgga agttttcccc aaaaaagttc caaccattgg aagttttttc
    10621 ccaaaaagtt ccaaccattg gaagttttcc gcttcagccg gaaaccgcgc cccttttccc
    10681 ggacttcgcg cagagggcga cgatgagaag ttcgatgagg agatcggagg agaccgggct
    10741 ggataccagc cgctcatggg ttttaacgat ttggcgcaga gcccgggcca gttcacggtc
    10801 tgtccagtac gcgcactgtt cggccagttt ctgaacacgg aaagagtgca tcttgcgggg
    10861 atcgtccttc cccagccgcg aaaggaccgc gtccgcttcc gggttttcgc tccaggggct
    10921 ttccccataa cccgctttcg ccctgcacca gcggcggttg agacagtccc ggatcagcgc
    10981 aagcatccgg atgtgcgatt gaagcggata gatgatggcc atggcgttca gtttttgcgc
    11041 ttggagattt ttcagcgatt tcagcgctgc aggcagattg cgcatcccca cgtgctcggt
    11101 cagctcaaag accggcgcct cgcggccggg agacaccagc aggcggacat gatccgccgt
    11161 cacggaggcc ccctcgccgc agctgactgc cagcttctcg atttccatgg ccatctgccg
    11221 ggtgtcgtag ccggtccgct ccagaaaagc ctcggcgaca gcctgtgaca ttttcagatt
    11281 cttttcgcgc agaagctccg cgaggcggcc ctgagcgtcc ttgaccgcct gccacggccg
    11341 gctttcaatc tcaaaggcat gcacctcgcc ggccttttca cacgccttgt agaaggccat
    11401 gcgccggctg acggccggcg aactgaccac cagatgctgc ccgggcggca ggccattttt
    11461 gatcaggtcg gtcaggcgct ccagttcatg tttgacccgt tcaatgcggc cgggatcgcc
    11521 ctcggaaaga aatgacgcgt tttcaagcca tacggttttt cctgcaccga gaaaaccgac
    11581 ggtcatgacc gcatccatgc atttgcggat ggtctccacg gcttcgtcag cctttgaggg
    11641 cgaaccatct atgcgctcca ggccaaaggc ctgttcagcg ggcgggcaca gttcatccac
    11701 gatggcccgg gctttggcgc tcacggcgaa ttcatcgccg ccgaggacca gatataccgg
    11761 cttgcggcct ccatcctgtt ccgatttcct tttgctgctc attttccgct ctcggcaatg
    11821 gccgacagtt tatccatctg caggtccgac aggccccact tttcccggag tgcccggtcc
    11881 caggtcattt ccccgtcaaa cacaaggcgc acaaattccc ccagcgattc cggaccgatc
    11941 agggacacaa taccccgcac ccattcttct gactgccggt agaaggcgcg gtttctgtca
    12001 gcattctgat cataagcctg tgcgctgaca agctcattca gggaccagag ggtttcgccg
    12061 gggaggcggg gctgaacgcg ggtgatgatt ttcccgccgc ccagatgcca ttccagcgtg
    12121 atctcccagc cgagatagct ggcgaggccc tcttccagcg caagcggttc gcgattagca
    12181 taaacctggc ggagacggaa atgcacaatc tcatgcggga tggagtcctc catttcagtt
    12241 tccgcatccg ggatgaaaag cacaaattca tttccgtact gcagggcgcg gccgtccgtg
    12301 cgccagccgc cgcgctgccg gacagttttc cagagattgg aatcggtgat caggaaacag
    12361 tgcgcgtgat tggtgagggg cggaatatcc agcgcgatct ccacttcacg ggaggcgtgt
    12421 tccgcctttc gcagaagcag ttcgaccagc tcagcgctgg gagcatagcc ggtgaaatgc
    12481 tccgtggcgg cgattttcca ttcgttcccg ccggccttca gcactccgcg gcccagccgc
    12541 gacatcccgt ccgtatccgc ccccagagcg tgaagagccc acagggtcaa accggcgccc
    12601 gcgccggccg ccgccagcag gagcagaagc attttcagaa tgtgccgcac ttttcccata
    12661 ggagagatgc actctagcgt gtccgggggc ggctgtcagc cacggaatcg cgcggattga
    12721 catcccccat cctttccttc atcctcgaat gatgatgatg aaaagaattt gggcgccgtg
    12781 gcggatgaaa tacattctgg gagcccggga aaccggctgc ttcctgtgcg ccatgcttcc
    12841 ggacgggaac gattcggaaa atctgatttt gaagcgcggg cggacctgtt tcattgtgat
    12901 gaaccgctat ccctacaaca acgggcacct gatggtttgt ccactgcggc atgtggattc
    12961 gctgggggcc atgacggcca aagagcgggc cgagatgatg gaactgacct ccgaatgcgt
    13021 tgcgctcctt cagaaggtga tgaagccgga cgggttcaat gtcggaatca acctcggagc
    13081 agcggcggga gccggcctga aggatcacat tcatatccac attgttcccc ggtggaacgg
    13141 cgacacgaac ttcatgccgg tgcttgggca gaccaaggtg attccccagg cgctccacga
    13201 acttcgcgac gagctcgccg cggccatgca ggcatgattt ttcagacaaa gaggatgctg
    13261 ctatgaaata caaacttctg ctgacagcct ggctctactc actggccctg cccgccgtgt
    13321 ccgcaacaca tctggacgag gcgaagctcg atgccctgca gcggcaggcc tttgactatt
    13381 tcctcgactg ccgggccacc aacgggctgg ttcccctgcg gctgggagac ccgcggctga
    13441 cctcaccgca ggccgaggga ctgcatctct cggcactcat cgccggaaca ggccggggct
    13501 ggctgaaacg gccggaagcc gcgcgccttg cggcgcagac actgagcacc tgcgcgcgac
    13561 tgccccggat acacggtttt ttccgcgaga gttacagcat ccacacgctg gaaccgctgc
    13621 ccgcgcagag ccgggcggat ttcatgggca cggcggcact catcgcagga gccctgacct
    13681 gtgaacagtt tttcgaccgt gaaacgccga ctgaaaacac catccgtgaa acagcgcggc
    13741 agctctatgc agaggcggag tggaactgga tgcttcggga caatgaaggc gagtccggat
    13801 gcaccccggc cctctactgg tccgaagacg gcggttattc tgatgaacgg gcggacggaa
    13861 gcgggtcact tcagggcatg ctggcctgtc tgctggcggc gggtgcgcct gcgcattcca
    13921 ttccggccgc gtgctggaac aaaggatggg cccgcggata tcagtgggag catatttccg
    13981 gcgcggatct cgtggtttct ccgccgctgt tctcgcagat ctatccgcaa atatggctgg
    14041 acctgaaaaa ccgaagagac cggcacacag actactggcg caacgcgttt catgccgtac
    14101 ggctgaatta ccgctactgc accagccggc tgtatcccgg aacagcattg tgggggctct
    14161 ccacctgtga aggcccgaaa ggcattgatg attacggctg cccgccgctg caggggcacg
    14221 tggatgaaga tgcggttctg gccccggcgg ccgccatttc cgccatcggc tgggcacctt
    14281 cagaagccct ggccatggtc gaggagatgg aaacaaacca cagggaggct gtttggggaa
    14341 aatacgggcc gcgggcctca ttcagccccc ggcacaactg ggtctccagg gactacctcg
    14401 ccactgacct cggcgcgatg gtctgcatga tcgagaacca ccggtccggg ctggtgcgcg
    14461 atctgttcag ctcacatgac gctgtgcgga gggccctcgc ggatacaggc ttcaccgggg
    14521 tcatcgccga tttcgaggaa gcgtcgggaa tggagcccta tctgacgtgg gaaagctcat
    14581 caactgcaat gcagaaaatc gcagcggata tctcccgtga aggcttccag tctctggagg
    14641 tggtctgccg ggaccgcacc ggaatgcttg cgggacggtc cgcacttagg gattttcaac
    14701 ccttcaggta tctgacgctc tggctgtcca gcggcagcga actggacatt gcgctggaag
    14761 attccggagg aagatccgtc cagcttgcac aggaaagcgc caaaggcgcc gaagacggct
    14821 ggacacgaca tgtatattcc atcgagggcg gcgaatgcga tctgacagat gtccgcagaa
    14881 tcctgtttac atttcatcct cagcgcccgg gggacccgct ctggatggac ggcatctttc
    14941 tggccgcaca gaaacccgga ccccggccgg aggggataca gaatctgagg gcagaatcat
    15001 cgcgaatgcc cggagaagca gttttgagat gggatccacc ggcggacgat gtattcacct
    15061 gccacgccag atattcggcc aggcccattc gcgattatga gtcgtttcag aaagccaccg
    15121 cggtcagcac cgctccgttc cggcacgcgg atcccgccag ggcggaaata catgttccga
    15181 acctcgtgcc cggagagacg tatcacttcg cggtgcagac cgaaaccctt gaccatgaga
    15241 aatccgagaa tacagccgtc accagcctga cgctgccccg cacgaaggct cccgatgagt
    15301 tccttgtgga tcatttcgac agcggcggca ttctgaactg gagaccggca ggaaccggga
    15361 cagaggtggc ggccgataca ggcaacgcac tgctcgggag cggcggctca ctgcgcattg
    15421 gagtttcaga cgcgaagaag gcatcaaagg cctccgccta tgccgacacg gatttccgcg
    15481 acttcagtca atacggatat ctggcccttt gggtgtcagg gaaaaccgcc atacaaatcg
    15541 ccctggcaaa tgaaaagggg gatacagaat atctgccgga acaaaagact gcgctggcgg
    15601 acggctggtc gccagtgttt tttgacctcg ccggcatccg caaactggac cggaagacca
    15661 ttagtcgcat actcattcat gtgcggcccg acaccgaaag ccgggaccgg ggcatttggg
    15721 tggattccct gcgcctgaca aaaacccgaa actgaacaga gaggctcgcg ccacctcagg
    15781 ggaggaaacc acgcgccaca tgggctgtac agacactgtg aacggccata tgcatggcgg
    15841 ccttccgcat attgagcctt ttcgtccgcg cggtgtcata tactcgttgg aagcggtcac
    15901 gcatccgctg ttcc
//
LOCUS       DAOQWR010000051        15847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4130_length_15847_cov_4.846821, whole genome shotgun sequence.
ACCESSION   DAOQWR010000051 DAOQWR010000000
VERSION     DAOQWR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_15847_cov_4.846821"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2366)
                     /locus_tag="PKK36_05735"
     CDS             complement(<1..2366)
                     /locus_tag="PKK36_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR94096.1"
                     /translation="MDEQGALEVDKMLREFIIHKPQTIRRPKELAKRMANLAHMVRDI
                     IVKALEQNEASDTLKDWRTAFAQTLMHDLMQPERTGDFADMFAQTIAYGLFSARVMDP
                     TPEDFSRQEAQALIPKSNPFLRNFFYQITGPDLDKEPFAPFVEDLVSVLGHTDMNRVL
                     EDFGVSKKTRDPVFHFYETFLAEYDPKLREQRGVYYTPDPVVSYIVRSVDHILKTKFK
                     CADGLADKSKVLVDNWKPENRTKKATQKKKVECHRVLILDPACGTGTFLYDIIGLIRN
                     QFMERNDAGMWPGYVKKSLLPRLFGFELMMAPYAIAHFKLSLQLAGRDLLELQAPGWK
                     YEMEDDDRLGIFLTNTLEDMHEMSGLPLFARWIAEETEAANEVKRDLPIMVICGNPPY
                     SGHSENKGAWISDKIEDYKFVNGQPLNERNSKWLQDDYVKFIRFAQWRLDESGNGILA
                     FISNNGYLDNPTFRGMRWSLMQSFNDIYILNLHGSSKKKETAPDGGKDENVFDIQQGV
                     CIGIFIKQPDSSRKCQIHYADLWGERQNKYDKLDKLDVSKTKWKKAEPKEPFFVFVPQ
                     DEAASDEYGKYWGVAEIAPLNVLGFQTHRDDFAIAYDQSVIRDRLSAMQATILTDDKF
                     AETYRLKDNRDWKVADARKNLRQVSDKESIIHQCNYRPFDQRWSCFSYVAMDYPRREL
                     LTNVAGRKNFCLGIGRQGMAVNDPVWSLLTCAKYPLDANVFRRGGVNVFPLYLYHDTE
                     ELNFGESGRKPNLSNKFIKEFSGKMGLEFIPDGQGDLKKTVGPEDVFY"
     gene            complement(3365..4057)
                     /locus_tag="PKK36_05740"
     CDS             complement(3365..4057)
                     /locus_tag="PKK36_05740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94097.1"
                     /translation="MPDHAKTYASEAERYERLIAREDCQQNILPAIRNICDPAGLEIA
                     DLGAGTGRLTCMLAPFAKHIWGFDSSGHMLQVAESRLRAAGLQNWTLGVADHQSIPLP
                     DASADLAVGGWALCYASARDGTDSTAGLNRAFEEIFRILRPGGTVILLDTLGTGNEEP
                     APLPKLREYYDYLTLGGFSFKWIRTDYYFDSVQEAVDLTRFFFGDVLAEKLENQQSRI
                     LPECTGVWWKKK"
     gene            complement(4059..5150)
                     /locus_tag="PKK36_05745"
     CDS             complement(4059..5150)
                     /locus_tag="PKK36_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94098.1"
                     /translation="MDLKQFEFFSFFDSRAAERLAELSEMLEIKKGGFIFREGDASDG
                     LYILVDGKIEICREDASGKRFQIALVEPNSYFGEFGVLDGQPRSAAAAAVEDSVLAKI
                     SNTDLEKIFAQTSAHSIIRFAADIVEKFRKSNAKYMEERLRQERISMVGQMVGEIIHD
                     YRNPFAVIALATEILGKKTGDEETVKFAGLIAEQVTRMQSMTDELLEFSRGETRFSRE
                     AVQVEDFLSHYELLNREYLAKQNVELKVTMIKKTIQADPAKLLRVLQNLTNNAVDCFK
                     GGPGKITVEARDLGDHVSFHVTDNGPGIPPEIAPRIFEAFATFGKKKGTGLGMAITKN
                     VIDAHGGEIGFDTAPGKGTTFNIKIPVGA"
     gene            5326..6624
                     /locus_tag="PKK36_05750"
     CDS             5326..6624
                     /locus_tag="PKK36_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94099.1"
                     /translation="MKWMNLLLCMLLIPVTGAMASETYRDGLIAYEKGEYEKAFEIFM
                     ALFRSNPGDEDLNYSMGLAAARAGRVSHAIFAYERALTLNPENGPARLELGRNWMSVR
                     QYDLARDEFGRLLALHPPEDIRRAAESELDRLAKFDRKWRFEGEATLSAIWDDNSNYG
                     PESDVIRTASGNYAADADIRPDETWGSAGSAKVLYTYDFGDANGWEYSLGGHGYIKRH
                     EKAPDEEISFIRADAILRHVRRDTLLELPLKVSAAEYGNSHLFNLLGSEPMFLYAVRP
                     KLQILARGNIEYRDYKRDDERDALFGEAGLILRRLPEGRQPLLSVQVHAFNEDAESEA
                     YDNDGWRAVAYGNVEVVSALYLYASVQYRETFYDAVLYPGYQAEPRDEEQMQYTVGLQ
                     STAIPHCTLDFSFRHTDNRANFDLYDYDKNVATISTTFTF"
     gene            6634..7575
                     /locus_tag="PKK36_05755"
     CDS             6634..7575
                     /locus_tag="PKK36_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNR94100.1"
                     /translation="MKTSAIFFILLAGALIIPSFAEAADPVGNVVAMEGRVSAAAGSS
                     GKVRTLGTGDPVYLNDVIKTAEKAKLQILFTDDTLFAMGENSEATISEYLFNPADKKK
                     NGMGISMGMGLFRVITGKITTLNPERFSLKSTRATIGIRGCELGFILTPKYDKIMIIR
                     VPKGKEIYIDDIPDVVSGRKGALFPPVRIFDGGTLLYLSDTDRPQKSKLSSEDINDIL
                     NGTGPGRGKSHAGQGLSHQGRGAGKSGAKGQHQLPGTKEVGDDPTGPLFEEEPGFVPP
                     VEPVDEPPTQRGGRSISRDPVPKPVWNGPDHSYHYGN"
     gene            7670..8989
                     /locus_tag="PKK36_05760"
     CDS             7670..8989
                     /locus_tag="PKK36_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94101.1"
                     /translation="MLLQRQWTLFFLFSLLIFNPGLQARANPLTTEAGMQAYNAGEYD
                     RAYDIFSELFRKDPQNVQINYGLGLSAAARGKLSHALFAFDRVLQVEPNNMRARLERA
                     RIYYSLGQYELARAEFSQVSGTRPPEQVQKNIGLFMEAMDRAERKYSFSARLDFRVVY
                     DDNLNFGPAAATIDTLLGELEVLGDSRPLEAWGLNTGVGAGGVYDFGERGGWLSYADA
                     GFSQDIYDDAPRQEITMLQGGAGLRRFGQITLLELPVRFISLDYGHDDLVDMAGIYPS
                     AVFMAASQWHLIGRLSLEDRNYKDNDLRDADFVQAAGTLRRLFDGGRASLSLNAGVFD
                     ENSDGDMFQNEGWTAGLSGDCRVSDWFTAYAGAQYRRSEYGAILYPDLQDVPREDDQW
                     QFIAGLRRELKKYWTVDLNHRHISNSSNFGLYDYDKNVTTVSTSLTF"
     gene            9002..11644
                     /locus_tag="PKK36_05765"
     CDS             9002..11644
                     /locus_tag="PKK36_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNR94102.1"
                     /translation="MKTATILMLAAVLFMAAGVLGAEPIGKVISLQGSVTATEEGGTE
                     RHLKIGSAVFQNDIIKTGPQSKVQLMFTDDTLFAQGAESEAHLTEYIFTPDQKSENAM
                     GMKLGLGMFRVITGKITDLNPDRFRVKTGRATIGIRGCELGFVLTRMADRVMIIRVPS
                     GREIIIDEIRDFGDATGPLRSSAIRNRRQGIAYTLQDGERPAQGRISSGDLLDLFENT
                     TPGDAGMEGGSETFDPSSSGQSNEENATGETPSSDESQTPSSPDNPPPADDSTTPAPG
                     DTSGTDSTPATGGTGDSSMTGNESFDPVVTDPTIDDPSLDGWTPEDGTTTDPEIPPSD
                     GAGTVPDPTVPPPPPTTLMLRGGGIGGTYLAGVPHNELQDVWIWKQLNGTMDNTAFDG
                     QLLGMHDNFFDSQPSDSIALTLNVPLQDFMPGSTIYDGHSAQTIGGATIDNDNLLQFW
                     TRVDSGDHTLALSYGGFTSENYAGAALPADSVIRYDILFTEFPPLRPTADPLTPVIEN
                     GTLWVNTRTGDFMVDPAMSPGLQGSADWLTFFGQEMQGVGHVGIPHADTSGNLQPGGM
                     CLAGFLNTSPGAVAPADTGTTIHNGGYAVGASFCEFPAGGSPVRAHAFYSGYFGESSV
                     SLVVNRDSPENNALLSIAAFQGPSSQSSDMFLTTPHQSFYVMDGLYEASYSQPNQLEG
                     VARGDVDSGGTDWGWGEWNGTRTTDYGDGPQEEMVEGRYVTGTTLTPAAYDTLFQGST
                     SFDLVGAGSASGYIGNNDYRSRVDGSASIHVQIPGGGAPANWDGTFQLSNANGDVLNV
                     SYSPLSLPISSSGNLTTTVTPGSYVFQAGGAPVASPLIEHRMDGSLVGPGTGAIPITG
                     AIGSGLFRHMDGHFAEFTYGTDLH"
     gene            11663..13825
                     /locus_tag="PKK36_05770"
     CDS             11663..13825
                     /locus_tag="PKK36_05770"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNR94103.1"
                     /translation="MKKWLPILAGLLVVFLWLLLWLANPRFLAAVSSQTYDMLLKLLA
                     EPRRSSHVVIADIDDKSIAELGQWPWPRFQLSRLVEKTFQAGAAVMVFDVVYSEKDRL
                     SPAQVLDTWTDIFGSDAQLAELLQGREDFDSMFASTVGRHPVVLGCYMNDRLAEQRAT
                     ADWSSWQSPYLEKGPRPADMLPLARDVVTPLSELASAGKIAFINTTADSDNIIRRTPL
                     IYEVAPEGIFPSLNLQAVRLFLQGPPYKIIYDSEGVEGVRQIVVGDLIIPTDRHGRIV
                     LNYRSDRFPHVSAIDILTGKHPVSLFSNRIVLVGTSAAALGDLVATPLAEDFPGIEVQ
                     ATAIENMLCGQTLWEPRWIVFLNMAVMVLLGVLLTLFISRRGALLSALVTLLAITATI
                     CSSAWLLQHFQLVANPTEISLCLAGVYIAVTFIRYLMEEGARKRMRTMFGPMVSPEVL
                     AWLEEHPDRVALAGHKQNATVFFSDIASFTTIAEQMDPAVLSRLMNRYLTTVTEIIMA
                     HDGYLNKFIGDGIMAVWGVPHDLEDHAVRACRAAVDQQRKLRAMAEELKAEFNVQLHV
                     RMGLNTGIVTAGNMGSSRRFEYTVMGDVVNRASRFEGINKEYATRIAIGQTTRDAIGS
                     VFPVRLLDLVVVKGETQPVRIYELLLPEDMESMAQTVAQYEAALKMHWERRWDESSAL
                     LLQNPQDGPSKALLARIEHFRSNPPPADWRGEDIKLTK"
     gene            complement(13837..14586)
                     /locus_tag="PKK36_05775"
     CDS             complement(13837..14586)
                     /locus_tag="PKK36_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR94104.1"
                     /translation="MIKLKTRQMIPCAFTIAALIAGLFSILKSASGDFVASAQFIMLS
                     MILDGMDGTLARLLRGTSRFGAELDTFVDITSFGTAPAVLLYLAVLKDFGFWGILLSS
                     VYISSGAIRLSRFRIVDPRRGQGGFLGLPITACAGWTALFVFVIYSHVLDEQMFSLSR
                     GPVAVFFWITTVAMLFLQVSQVHYAKPTKELVMFVPSIFFVGFLFLHASVAIAAAIAM
                     IAYGIVYVFISPMLPKHVVVEDEEEPLTAEN"
     gene            complement(14598..15260)
                     /locus_tag="PKK36_05780"
     CDS             complement(14598..15260)
                     /locus_tag="PKK36_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNR94105.1"
                     /translation="MKTGIVKCGWVWIIPPFVLGAAGWWLLTRAGYGAAGWTAAAAGL
                     VLGSFMIYFHRDPQRIPPQEEGIILAGADGVIRRVEELHEPDYLQGPAVRISIYLNPF
                     DVHVNRSPLGGRVTRLAYTPGKHMLTIMNAASEVNEHSSIFIDNPRICCLVRQIVGPI
                     VRRVVYWLKEGQEIAPGQTIGMMRFGSRLDIYLPSSRVKVIVKKGDRVSAGLTVVARI
                     IN"
     gene            complement(15335..15769)
                     /locus_tag="PKK36_05785"
     CDS             complement(15335..15769)
                     /locus_tag="PKK36_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94106.1"
                     /translation="MNGRDISTTGHVQGFVLAYELRGHMLTYRFLSLIPLARIDLRDA
                     VQIRLSSFSEYMKTLGAHPWGTRFWWTCGLRCRDSEAPVYLIRTCRKKRSCFVRLKAG
                     MHYRLRVAIHRWSAYYTDAEGRNAVLCANDESRPQAEQLTAL"
BASE COUNT         3462 a         4437 c         4442 g         3506 t
ORIGIN      
        1 taataaaaca catcctccgg cccgacggtt ttcttcaaat ccccctgccc gtccggaatg
       61 aattccagcc ccatcttccc gctgaattct ttgatgaatt tgttcgacaa gttcggcttg
      121 cgccccgatt cgccaaagtt aagttcttcg gtatcgtggt aaaggtagag ggggaaaaca
      181 ttcactcctc cccgacggaa tacatttgca tccaacggat acttggcaca cgtcagcaac
      241 gaccacactg gatcatttac agccattcct tggcgaccga tccccaaaca aaagttcttc
      301 cgtccagcaa cattggtaag cagttcgcgg cgagggtaat ccattgccac ataactaaag
      361 cacgaccagc gctgatcaaa aggacggtag ttacattggt gaattatcga ttctttgtct
      421 gaaacctgcc ggagattttt tctggcatcc gctactttcc aatctcgatt atctttcaac
      481 ctataggttt cagcaaattt atcatcagtc aatatcgttg cttgcatagc agataagcga
      541 tcacgaatta ctgattgatc gtaagctatc gcaaaatcat ccctgtgtgt ctgaaatcct
      601 aaaacattta gtggtgcaat ttcagccact ccccaatact tgccgtattc atcagaagca
      661 gcttcatctt gcggcacaaa aacaaagaag ggttctttgg gctcggcctt cttccacttc
      721 gtcttgctga catcaagttt atcgagtttg tcgtatttgt tctgccgttc gccccagagg
      781 tcggcgtaat ggatctggca tttacgactt gaatcaggct gtttgataaa aatgccaatg
      841 cagacgccct gctggatgtc gaagacgttt tcgtctttgc caccgtctgg cgcggtttcc
      901 tttttcttgg agcttccgtg caggttcagt atgtagatgt cattgaaact ctgcatgagc
      961 gaccagcgca tgccgcggaa ggtcgggttg tcgagatagc cgttgttgga gatgaaggcg
     1021 aggattccgt tgccgctctc gtccagccgc cactgcgcaa agcggatgaa tttgacgtag
     1081 tcgtcctgaa gccattttga attccgctca ttgaggggct ggccattcac gaatttgtag
     1141 tcttcgatct tgtcgctgat ccacgcgccc ttgttttcgg aatggccgga atacggcggg
     1201 ttgccgcaga tgaccatgat gggcaggtcg cgcttgacct cgttggcggc ttcggtttct
     1261 tcggcgatcc agcgggcgaa gagcggcaag ccgctcattt cgtgcatgtc ctcaagtgtg
     1321 ttggtgagga aaatgccgag gcggtcgtcg tcctccattt cgtatttcca gcccggcgcc
     1381 tgcagctcca gcaggtcgcg cccggcaagc tgaaggctca gcttgaaatg ggcaatggcg
     1441 tatggcgcca tcatcagctc gaacccgaac aggcgcggga gcagggattt tttgacatag
     1501 ccgggccaca tgcccgcgtc gttgcgctcc atgaactggt ttcggatcag cccgatgatg
     1561 tcataaagaa aagttccggt tccgcaggcg gggtcaagaa tcaggacgcg gtggcattcc
     1621 acttttttct tctgcgtcgc ttttttggtg cgattttcag gcttccagtt gtcaacgagc
     1681 actttggact tgtccgccag tccgtccgcg catttgaatt ttgttttcag aatgtggtcc
     1741 acggaacgga cgatatagga aacaaccgga tcgggcgtgt aatagacgcc gcgctgttcg
     1801 cgaagtttcg gatcgtattc cgcgagaaag gtttcataga aatggaaaac cggatcgcgg
     1861 gttttcttcg agacgccgaa gtcctccagc acccggttca tgtcggtatg ccccagcacg
     1921 ctcaccaggt cttcaacaaa cggcgcaaaa ggctctttgt ccagatccgg gccggtgatc
     1981 tgatagaaga aattccgcag gaacggattg gatttcggga tcagcgcctg tgcctcctgg
     2041 cgcgagaaat cttccggggt cgggtccatc acgcgggccg agaacaggcc gtaggcaatg
     2101 gtctgcgcga acatgtcggc aaaatctccc gtgcgttcgg gctgcatcag gtcatgcatg
     2161 agtgtctgcg caaaggcggt gcgccagtcc ttcaaggtgt cggatgcttc attctgctcc
     2221 aatgctttga cgatgatgtc gcgcaccatg tgggcgaggt tggccatccg cttggccagc
     2281 tctttgggcc tgcgaatggt ctgcggcttg tgaatgatga actcccggag catcttatca
     2341 acttccagtg cgccctgttc atccatccgc acattatttc cggaaagata ccccgcgcgg
     2401 gcggtctgcc gccattcccc gttcacatac cagcggaatt cgagataatc ggtcaggatc
     2461 aggttttcca gcgccgggag atagcgcttc aactgggcgc tcttttcaat ctcattgagc
     2521 gaaacgccga tgtctttggc ttcgatatag cccagcgtca gattgcgacg ggaaatcaga
     2581 taatccggcg cgccgcaggt gtctcgcctg ggttcattca cggcttgaat gcctttatcg
     2641 agggattcca tcagattctt gagggccggc cggtaggtgt gttccgtggc aatgccgcgc
     2701 gaatgctctt tttcaaccgc cttgagataa tcctgaatgc atgctgtcat tgcttcagga
     2761 tattgacttc ctcctcgtca aaatgcgagc aggtttttta tttgtatcgg gtgaggcgac
     2821 cggaggaaat cgtctaatgc agtagccgct ctgtgagcgg catcttagaa actgcgcctc
     2881 acagaggcgc tactacaact gcgcacccgg ccctgtccgc aattcattcc acaaagcggg
     2941 gtcttggttt gccctcggcg tccatccagg ttttctcaag cctggtcgag caacagatca
     3001 ctgtgtaatc aaaccgcatg agatagaaag gaggcggaat gcgcgtcctt ccaagattat
     3061 taacatggcg actagatcgc atcccgtctc tctagctcac acttgagaag catactccct
     3121 ctccggtccg tcgcaaattg aatttttcag ttttaatccc gtagatcctg taaatccttg
     3181 tctgaatcta aattctccgc gtcactgcgt ttttgcgcgc attttcatgg ttttcttcat
     3241 tttttggccg tttttcggtg tttttgggcc attttttgcg tttttttagc attttttggc
     3301 cttttttcgg gatccggtcg cgacagaacg ccccccatga cgaggagatg gtgaagcgaa
     3361 cggactattt tttcttccac cagacgccgg tgcattcggg caggatgcgt gactgttgat
     3421 tttccagctt ctcagccagc acatcgccga agaagaagcg tgtgaggtca acggcttcct
     3481 gcacggagtc aaagtagtag tcggtcctga tccatttgaa ggaaaatccg ccgagggtca
     3541 gatagtcata gtattcgcgg agctttggaa gcggagcggg ctcctcgttc ccggtgccca
     3601 gcgtgtcgag caggatcacg gttccgccgg gacgcaggat gcgaaagatt tcctcgaagg
     3661 cccgattcag cccggctgtc gagtctgtgc cgtcgcgcgc cgaggcatag cacagggccc
     3721 agccgccgac cgcgaggtcc gcggatgcat ccggcagagg gatgctctga tgatccgcca
     3781 ctcccagagt ccagttctgc aggccggcgg cgcgcagccg ggactccgcc acctgaagca
     3841 tgtggcccga tgaatcaaat ccccagatgt gctttgcgaa gggcgcgagc atgcaggtga
     3901 gacgccccgt gcccgcgccg agatccgcga tttccagtcc cgccggatcg cagatattcc
     3961 tgatcgcggg caggatattc tgctgacagt cctcgcgtgc aatcagccgc tcgtagcgct
     4021 cggcttcgga agcgtaggtc ttcgcatggt ccggcatgtc aggcgccgac gggaatcttg
     4081 atattgaagg tggtgccctt ccccggcgct gtgtcgaagc cgatttcgcc cccgtgggcg
     4141 tcaatgacgt tcttggtgat cgccatgccc aggccggtgc cttttttctt tccgaaggtg
     4201 gcaaaggcct caaaaatgcg cggagcgatt tcggggggga tgcccgggcc gttgtccgtg
     4261 acatggaagc tgacatggtc gcccagatcg cgggcttcga ccgtgatttt tccgggcccg
     4321 cccttgaagc agtccacggc gttgttggtg agattctgca gcacccgcag aagtttcgcc
     4381 ggatcggcct gaattgtttt tttgatcatc gtgaccttca gttccacgtt ctgcttggcc
     4441 aggtactcgc ggttcagcag ttcataatga ctgaggaaat cctccacctg aacggcctcc
     4501 cggctgaagc gggtttctcc gcgcgagaat tccagaagtt catccgtcat gctctgcatc
     4561 cgggtgacct gttcggcaat cagcccggca aatttgacgg tctcctcgtc accggttttc
     4621 ttcccgagga tttctgttgc gagcgcaata accgcaaaag gattccgata atcgtgaatg
     4681 atctcgccca ccatttgtcc gaccatcgag atgcgttcct gccgcaaacg ctcctccatg
     4741 tatttggcat tggatttgcg gaatttttca acaatgtccg ccgcaaagcg gataatggaa
     4801 tgggccgagg tctgggcaaa tatcttttcg aggtccgtgt tggatatctt ggccagcacg
     4861 gaatcttcga ctgcggcggc ggcggcgctg cgcggctgtc cgtccagcac cccgaactct
     4921 ccgaaatagc tgttgggttc aacaagcgca atctggaacc gtttgcctga ggcgtcctcg
     4981 cggcagattt caatcttgcc gtccaccagg atatacaaac cgtcggacgc atcgccctcg
     5041 cggaagataa agcctccttt tttgatctcc agcatttcgg aaagctccgc caggcgctcg
     5101 gccgcccggc tgtcaaaaaa ggagaaaaat tcaaactgtt tcaggtccat ggttccatcc
     5161 cctctttctc cactgtttca ctggagactt tataacggag cgggcccgca aagtcgagcg
     5221 cgggatagcc gattcttctt tgtttggctg tacaatttca catggtttgt tcatactaag
     5281 aatcgaggaa ggcaggattt gaaaaccgga tatgcggggt gttctatgaa atggatgaat
     5341 ctgctgctgt gcatgcttct gattccagtg acgggggcaa tggcctcgga gacttatcgg
     5401 gatggtctca tcgcatatga gaagggcgaa tacgaaaaag cctttgaaat tttcatggcc
     5461 cttttccgga gcaatcccgg ggatgaagac ctgaactact ccatgggcct ggcggccgcg
     5521 cgggccggga gagtctcgca tgccattttt gcctacgagc gcgccctgac tctcaatccg
     5581 gaaaacggcc cggcacggct tgaactgggc cgcaactgga tgtctgtcag gcagtacgac
     5641 ctggcaaggg atgaattcgg ccggctgctt gctcttcatc cgcctgaaga tattcgccgg
     5701 gccgcagaaa gcgaactgga ccgtctggcc aagtttgacc ggaaatggcg ctttgaggga
     5761 gaagccactc tttccgccat ctgggacgac aacagcaatt acgggccgga atccgatgtg
     5821 atccggacgg cgtccgggaa ctatgccgcg gatgcggaca tccgcccgga cgaaacctgg
     5881 ggcagtgccg gaagcgcaaa agttctttac acctatgatt ttggcgatgc gaacggatgg
     5941 gaatattcgc tgggcggcca cggctatatt aaacgtcatg aaaaggcccc ggatgaggag
     6001 atatcgttta tcagagctga cgccatactg cgtcatgtca ggcgggacac tctgctggaa
     6061 cttccactga aggtcagtgc cgccgaatac ggaaacagcc atctgttcaa tctgctgggc
     6121 tccgaaccga tgttcctcta tgccgtcagg cccaaactgc agattctcgc acgcggcaat
     6181 atcgaatacc gcgactacaa gcgggatgat gagcgcgatg ccctgttcgg agaggccggc
     6241 ctgattctgc ggcgcctgcc cgaagggcgg cagccgctgc tttcagttca ggttcacgca
     6301 ttcaatgaag acgccgaaag cgaagcttat gacaatgacg gatggcgggc cgtcgcttat
     6361 ggcaatgttg aggtggtcag tgcgctctat ctttatgcca gcgtgcagta ccgcgagaca
     6421 ttttatgatg cggtgctgta tccgggctat caggcagaac cccgtgacga ggagcagatg
     6481 cagtacaccg tgggactgca gtccacggcc atcccccact gcacactgga tttcagtttc
     6541 aggcacacgg acaatcgggc gaatttcgat ctttatgatt atgataagaa cgtggccacc
     6601 atttccacaa cattcacttt ctaggaggtt cacatgaaaa catccgccat ctttttcatc
     6661 ctgctggccg gcgcattgat catcccctca ttcgcggagg cggctgatcc cgtcggaaac
     6721 gttgtggcca tggagggccg ggtttccgcg gcagccggtt catccgggaa agttcggacg
     6781 ctgggaacag gcgatcccgt ctacctgaac gatgtcatca aaacagccga gaaagccaaa
     6841 ctgcagattc ttttcacgga tgacacgctt ttcgcgatgg gtgaaaacag cgaagcgaca
     6901 atcagcgaat atctgttcaa tcccgcggac aaaaagaaaa acggaatggg catcagcatg
     6961 ggcatggggc tgtttcgagt aatcacgggt aagatcacca ccctcaatcc ggaacgcttc
     7021 agcctgaaaa gcactcgtgc caccattgga atacgcggct gcgaactggg gttcatcctg
     7081 acgcccaagt atgacaaaat catgattatc cgggttccga agggcaagga gatctatatt
     7141 gacgacatcc cggatgtcgt atcaggccgg aagggcgcac tgtttcctcc cgtccgcata
     7201 ttcgacgggg gcacactgct ctatttgagc gacaccgacc gcccgcagaa aagcaagctg
     7261 tcgagcgaag acatcaatga tatcctcaac ggcacggggc ccggccgggg gaaaagccat
     7321 gccgggcagg gattgagcca tcagggccgg ggcgcaggga aaagcggagc gaagggccag
     7381 catcagcttc cgggaacgaa ggaagtgggc gatgacccca caggcccgct ttttgaggaa
     7441 gaacccgggt tcgttccgcc agttgagccg gtggatgagc cgccgacaca gcgaggcggc
     7501 cgcagcatca gccgcgatcc cgtccccaaa cccgtctgga acgggccgga tcattcttat
     7561 cattatggaa attgagcctg ccggggcaaa aaggcgaatt ttaagctttc acctgatgca
     7621 ggggtatctc tataatcctc accgcaaagt agatatgtga ggaggcatca tgctgctgca
     7681 aaggcaatgg acgttgttct ttcttttttc gcttttgatt ttcaacccgg ggctgcaggc
     7741 gcgcgcaaat cctcttacga cggaagccgg catgcaggca tacaatgccg gcgagtatga
     7801 tcgcgcatac gacatcttct ccgaactgtt ccgcaaagac ccgcagaatg ttcagataaa
     7861 ctacgggctg ggcctttcgg cggcggccag aggcaaactg tcgcatgccc ttttcgcctt
     7921 tgaccgtgtg cttcaggtcg aaccgaacaa catgcgcgcg aggctggaac gggcccgcat
     7981 atattattcc cttggccagt atgaactggc gcgggctgaa ttctcgcagg tttcgggcac
     8041 ccggccgccc gaacaggtgc agaagaacat tggtctgttc atggaggcca tggacagggc
     8101 cgaacggaaa tactcgttca gcgcgaggct cgattttcga gtggtatatg atgacaatct
     8161 gaacttcgga cccgccgctg ccaccattga caccctcctg ggcgagctgg aggttctggg
     8221 tgacagccgt ccgctggagg catggggcct gaacacgggg gtcggggcgg gaggagttta
     8281 tgattttggc gagcgcggcg gatggctgag ttacgccgac gcaggatttt cacaggacat
     8341 ctatgatgat gcgcccaggc aggaaataac catgctgcag ggcggcgcag gtttgcggcg
     8401 cttcggacag atcaccctgc tggaactgcc cgtgcgattc atcagtctgg attatggaca
     8461 cgatgacctc gtggatatgg ccgggatata tccttcagcc gtgttcatgg ccgcatccca
     8521 gtggcacctg atcgggcgtc tcagtctgga ggaccgcaac tacaaggaca acgatttgcg
     8581 cgatgcagat ttcgttcagg cggcaggaac actgcggcgc ctatttgacg gcgggcgggc
     8641 ctcgctcagc ctcaacgcag gtgtcttcga cgaaaactct gacggggaca tgttccagaa
     8701 cgagggctgg accgcgggcc tgtcaggaga ctgccgcgtg agcgactggt tcacggccta
     8761 tgccggagca cagtaccggc ggtctgaata cggtgcgatc ctttatccgg atctgcagga
     8821 tgttccgcgc gaggatgatc aatggcagtt cattgccggc ctgcgccggg aactgaaaaa
     8881 atattggacc gttgatttga atcaccgtca tatcagcaat tcttcgaact tcggcctgta
     8941 tgactacgac aagaatgtga ccacagtttc gacatcgctg actttttagg ggagaagcca
     9001 tatgaaaaca gcaactatac tcatgcttgc agcagtcctc ttcatggcgg ccggggtact
     9061 gggcgccgaa cccattggca aggtgataag ccttcagggc tccgtcacag cgacggagga
     9121 aggcggaacc gaacgccatc ttaagatcgg ttccgcagtc ttccagaacg acatcatcaa
     9181 gacgggaccg caatccaagg ttcagctgat gtttaccgat gacaccctgt ttgcgcaggg
     9241 tgccgagagt gaagcccatc tcactgaata tatattcacg ccggaccaga aaagtgaaaa
     9301 tgccatgggc atgaagctgg ggcttgggat gttccgtgtc atcaccggaa aaatcaccga
     9361 cctgaatccc gaccgcttca gggtgaagac gggacgagcc accatcggca tacgcggctg
     9421 tgaactcgga tttgtgctga ctcgcatggc tgaccgggtc atgattattc gtgtgccttc
     9481 aggccgcgaa atcattattg atgaaatcag ggatttcggc gacgcaacag gcccgctgcg
     9541 ctcttcggcc atccgcaacc gcaggcaggg tattgcctat acgcttcagg atggcgaacg
     9601 ccccgctcaa ggaagaatat cgtccggaga cctgctggat ctgtttgaaa acaccactcc
     9661 gggagatgcc ggcatggaag gcggatcaga gacattcgat ccgtcctcgt ccgggcagag
     9721 taatgaagag aatgcaaccg gagaaactcc ctcttctgat gagagccaga cgccttcttc
     9781 acccgacaat cctccgccgg cagacgacag cacgacacct gcgcctggcg acacttccgg
     9841 aaccgactca actcctgcaa cagggggaac gggtgactcc tccatgactg gcaatgaatc
     9901 cttcgacccc gtagtcacag accccactat tgatgatccc agtcttgacg ggtggactcc
     9961 ggaagacggg acgacaacag atccggaaat ccctccgtct gatggcgcag gcaccgtgcc
    10021 agatccgacg gttccgcccc cgcctccgac tacgctcatg ctgcggggcg gaggcattgg
    10081 cggcacctat ttagccgggg tgccccacaa cgagctgcag gatgtctgga tttggaagca
    10141 gctgaatggg acaatggaca ataccgcctt tgacggacaa cttctgggca tgcacgacaa
    10201 cttcttcgac agccagccgt cagacagcat tgcactgacc ttgaatgttc cgctccagga
    10261 cttcatgccc ggcagcacca tctatgacgg gcatagtgca caaaccatag gaggcgcgac
    10321 cattgacaat gacaatctgc tgcagttctg gacgcgtgtc gacagcgggg accacacgct
    10381 ggctttaagc tatggcggtt tcacttcaga aaactacgcc ggcgccgcac tgcccgcgga
    10441 ttcagttatt cggtatgaca ttctcttcac agagttcccc ccccttcgtc ccaccgcaga
    10501 tcctttgacc ccagtaattg aaaacgggac attatgggtg aacacgcgaa caggagattt
    10561 catggtggat ccagccatgt cgccaggcct tcagggcagt gctgactggc tgaccttttt
    10621 cgggcaggaa atgcagggcg tcggacacgt gggcatccct catgctgaca catcaggcaa
    10681 tctgcagccc gggggcatgt gcctggcggg atttctgaac acaagcccgg gggctgttgc
    10741 cccggcggat acggggacga ccattcacaa cggcggctac gctgtgggcg catctttctg
    10801 tgaattcccc gccggaggct cgccggttcg cgcacacgcg ttctattccg gctatttcgg
    10861 cgagagcagc gtttcgcttg tcgtgaacag ggattcgccc gagaacaatg ccctgctgag
    10921 tattgccgct tttcagggcc cgagttctca gtcgtcagac atgttcctta cgacacctca
    10981 tcagagcttc tatgtgatgg atgggcttta tgaggccagc tattctcagc cgaaccagct
    11041 tgagggcgtt gcccgcggcg atgtggacag cggcggaaca gactggggct ggggagaatg
    11101 gaacggaacc cggaccacgg actacggcga tggcccccag gaggaaatgg ttgaaggccg
    11161 gtatgtgacc ggaaccacgc ttacgcccgc agcttacgat accctcttcc agggctccac
    11221 ttcatttgat ctggtcggcg caggttctgc ctcgggttat atcggcaaca atgattaccg
    11281 ctcccgcgtg gatgggtcgg ccagcattca tgtacagatt cccggcggag gcgccccggc
    11341 caactgggac gggacattcc agctgagcaa tgcaaatggg gatgtgctga atgtttccta
    11401 ctctcctctg tcattgccga tttcttccag cgggaatctg accaccaccg ttacacctgg
    11461 ttcttatgtg ttccaagcgg gcggcgctcc cgtagcatcc cctctgatcg aacaccgcat
    11521 ggacggcagc ctcgtgggtc ccggcacagg ggccatcccc atcaccggag ccatagggtc
    11581 tggacttttc cgtcatatgg acggacattt tgccgaattc acttatggca cagaccttca
    11641 ttaaaactgg aatttcagac gggtgaagaa atggctcccc attctggccg ggctgctggt
    11701 ggtttttctc tggcttctgc tctggctcgc caatccgcgg tttctggccg ccgtttccag
    11761 ccagacctac gacatgctgc tgaagctgct ggcagagcct cgccgcagca gccatgtcgt
    11821 cattgcagat attgatgaca agagcatagc ggaactggga caatggccgt ggccccgctt
    11881 ccagctctcc cggctggtgg agaagacctt tcaggccggc gcggccgtca tggtgtttga
    11941 tgtggtctac tccgagaagg atcgtctctc tccggcgcag gtcctggaca catggaccga
    12001 cattttcggc tctgacgcgc aactggcgga gctgctccag gggcgagagg actttgattc
    12061 aatgttcgca tccacggtcg gccgtcaccc ggttgtactc ggctgttata tgaacgacag
    12121 gctcgcggaa caacgggcga cagccgactg gtcatcatgg cagagtccct atctggaaaa
    12181 agggccgcgc ccggcagaca tgctgccgct ggcgagggat gttgtgactc cgctttccga
    12241 actggcttct gcagggaaaa tcgcctttat caacaccacg gcagacagcg acaacataat
    12301 tcgccgaacc cctctgatct atgaagtggc accggagggc attttcccgt cgctgaacct
    12361 tcaggccgtc cggctgtttc tccaggggcc gccttacaaa atcatttatg acagcgaggg
    12421 cgttgagggc gtccggcaaa ttgttgttgg cgacctgatc attcccacag accgtcacgg
    12481 ccgcattgtg ctgaactacc gttctgaccg tttcccgcac gtcagcgcga ttgatattct
    12541 gacagggaaa catccggtga gtcttttctc caaccgcatt gtactggtgg gcacctccgc
    12601 ggccgcactg ggcgatctgg tggccacccc tcttgcggag gattttccag ggattgaagt
    12661 gcaggccacg gccattgaga acatgctctg cggccagacc ctgtgggagc cccgctggat
    12721 cgttttcctg aacatggccg ttatggtgct tctcggggtc cttttgacgc tgttcatcag
    12781 ccgccgggga gcgcttctgt ccgcgctggt gacactcctg gccatcactg cgaccatttg
    12841 ttccagcgca tggctgctgc agcactttca gctggtggcc aaccccactg aaatatccct
    12901 gtgcctggcc ggagtatata ttgcggtcac gttcatcagg tatctcatgg aagaaggggc
    12961 ccgaaagcgc atgcgcacca tgttcggccc catggtctcg ccggaagtgc tggcctggct
    13021 tgaagaacat cctgaccggg tcgcccttgc cggccacaag cagaacgcca cggttttctt
    13081 ttcagacatc gcttcgttca ccaccattgc cgaacagatg gatcccgccg tgctttcgcg
    13141 cctgatgaac cggtatctga ccacggtcac ggaaatcatc atggcgcatg acggatacct
    13201 gaacaagttc attggtgacg ggatcatggc ggtctggggc gtgccgcacg acctggagga
    13261 tcatgccgtt cgggcctgtc gtgccgctgt cgaccagcag cggaagctca gagcaatggc
    13321 tgaagagctg aaggcggagt tcaatgtgca attgcatgtg cggatggggc tcaatacagg
    13381 cattgtcacg gccggcaaca tggggtcgag ccgccggttt gaatatacgg tgatgggaga
    13441 tgttgttaac cgcgcctcgc gctttgaagg catcaacaag gaatatgcaa cgcgcattgc
    13501 cataggccag accacgcgtg atgccatagg cagtgttttt cccgtccgcc ttctggatct
    13561 ggtggtggta aaaggcgaaa cgcagcccgt gcgcatctat gaacttctgc tccctgaaga
    13621 catggaatcc atggcgcaaa ccgttgcaca gtacgaagcc gcgctgaaga tgcactggga
    13681 gcgccggtgg gatgagtcat ctgccctgct cttgcagaac ccgcaagacg ggccgtcaaa
    13741 ggcgctgctg gcccgcattg aacacttcag atcgaatccc cctcctgcgg actggcgggg
    13801 tgaagatatc aagctcacca aataagccgc gactgctcag ttttcagccg tcaggggttc
    13861 ctcttcgtct tcaaccacca cgtgtttagg cagcatgggc gaaatgaaga catagacgat
    13921 tccatacgca atcatggcaa tggccgccgc tatggccaca ctggcgtgca ggaaaagaaa
    13981 ccccacaaaa aaaatgctgg gaacaaacat taccagttcc tttgtaggtt tggcataatg
    14041 gacctgagac acctgcagga agagcatggc cacagtggtg atccagaaaa acaccgccac
    14101 ggggccgcgg ctaagtgaaa acatctgctc gtcaagaaca tgggaataaa tcacaaaaac
    14161 aaacaatgcg gtccagccgg cgcaggcggt gatcggcagc ccgagaaatc cgccctgccc
    14221 ccgccgcgga tccacaatcc tgaatcgcga aagccggatg gcgcctgaac tgatatagac
    14281 cgaggaaagc agtatccccc agaacccgaa atccttcagc acagccagat aaagcagaac
    14341 ggcgggcgcg gtgccgaagc ttgtgatgtc cacaaaggtg tccagttccg ccccaaagcg
    14401 ggaagtgccc cttaaaagcc gggccagcgt gccatccatg ccgtcgagga tcatggacag
    14461 catgatgaac tgggcagacg ccacaaaatc gcctgatgcg cttttcagta tgctgaaaag
    14521 tccggcaatc agcgccgcaa tggtgaatgc gcagggaatc atctgtcttg tcttaagttt
    14581 tatcacgaaa tcagtcctca attgatgatg cgggccacga ccgtcagtcc tgcagagacc
    14641 cgatcacctt ttttgacgat aacctttacg cgggatgagg gcaaataaat atcgaggcgg
    14701 gaaccaaatc tcatcattcc gatggtttgc ccgggcgcaa tctcctgtcc ttccttcagc
    14761 cagtacacca cccgccggac aatggggccg acgatctgcc ggacaagaca gcatatgcgg
    14821 gggttatcga taaaaatgga actgtgttca ttaacttcag aggccgcatt catgatggtg
    14881 agcatatgtt tccccggagt gtaagccagg cgtgttacgc gcccgcccag cggggaacgg
    14941 ttcacatgca catcgaacgg attaagataa atgctgattc gaacggcagg cccctgcagg
    15001 taatccggct cgtgaagttc ttctacccgc cgaatgactc cgtcggcgcc agccaggatg
    15061 atcccctcct cctgcggggg aatgcgctgc ggatcccggt ggaaataaat cataaacgag
    15121 cccagcacaa gacctgcggc ggcggcagtc catcccgccg cgccatagcc ggcccgcgtc
    15181 aggagccacc atcccgccgc gcccagcaca aacggcggaa tgatccacac ccagccgcat
    15241 ttcacaatcc cggttttcat gtcactataa gttcctcaaa cgtctgaaca gaataagcct
    15301 cgaccgggca ttgaaaagaa aatattcagg gaatctacag ggccgtcaac tgctccgcct
    15361 gcggccggga ttcatcgttt gcgcataata ccgcgttccg cccttcggcg tccgtataat
    15421 aagccgacca gcggtgtata gccactctca ggcgataatg catccccgcc ttcaaccgga
    15481 caaagcagct tcttttcttc ctgcaggtgc ggatcaggta gaccggcgcc tcggaatcac
    15541 ggcagcgcag accgcaggtc caccagaacc gggttcccca aggatgcgct ccgagggttt
    15601 tcatgtactc actgaaggaa gaaagccgta tttgcacggc atcccgcaga tcaattcggg
    15661 caagcggtat aagggaaaga aatctatagg tgagcatgtg cccgcgcagc tcgtacgcca
    15721 gcacaaaccc ctgcacatgc cctgttgttg agatatccct cccgttcatg gccagacagc
    15781 ttacccgtga ccccaaatga agttaagggg aaaatggtgg agcggaaggg gatcgaaccc
    15841 tcgacct
//
LOCUS       DAOQWR010000052        15193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4384_length_15193_cov_4.990752, whole genome shotgun sequence.
ACCESSION   DAOQWR010000052 DAOQWR010000000
VERSION     DAOQWR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15193
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_15193_cov_4.990752"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKK36_05790"
     CDS             <1..348
                     /locus_tag="PKK36_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94107.1"
                     /translation="WIFREIKSLWESGEASPPPSLQERRAMIERHLKLSVEHALIENK
                     YRKRRRNTAEQAACCYFRAHLVRYLAGLNGYRHILLRMECMNSVEEIMKEVDLLIERN
                     LNLEMEHGRNEES"
     gene            326..574
                     /locus_tag="PKK36_05795"
     CDS             326..574
                     /locus_tag="PKK36_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94108.1"
                     /translation="MEETKNLEHGTQPLDALLNGLEIANGDLVRASTEHLTFKVVQKG
                     RRGRELTMRAQLKIVNALNRLRPDDPVRPADLFNYRGR"
     gene            633..1082
                     /locus_tag="PKK36_05800"
     CDS             633..1082
                     /locus_tag="PKK36_05800"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNR94109.1"
                     /translation="MSDTTLSELLWRYDPSGIVVADQDMIIRQVNPAFCRIFRVDADT
                     ITGQPLSRALREEPRYFISAWNDNRPLRGHDKLYMDNNAYVRHLIVPLKENGLVVGIL
                     TDMTEEWRRRQMLETARKQIMEEQQALGHPDGSRLPAILKTVEELLA"
     gene            complement(1087..1161)
                     /locus_tag="PKK36_05805"
     tRNA            complement(1087..1161)
                     /locus_tag="PKK36_05805"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1127..1129),aa:Asn,seq:gtt)
     gene            complement(1234..2067)
                     /locus_tag="PKK36_05810"
     CDS             complement(1234..2067)
                     /locus_tag="PKK36_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNR94110.1"
                     /translation="MEITVGIFLVLMAMAFVCAFIDSSMGMGYGTILGPLLLIMGFSP
                     TVVVPAILLTQAFEGFVASMFHQQFENVSFEPSSREWKMLIMVVGFGIVAAVMAAVIA
                     VNIPKVVLKTYIGLLVTAMGIILLTRKSFTFTWNRMVFVGILSAFNKGMTGGGFGPVI
                     TGGQVMAGQEHKAAVGITTMAQAPICISGFFAYMIFRTVREVPGAVLEMRLADFFTRM
                     FSPQLFQWELILALFIGSIMVAPFGAFTTRSLKQASIHYMMGGLITVLGIWTLARTWM
                     P"
     gene            complement(2438..3547)
                     /locus_tag="PKK36_05815"
     CDS             complement(2438..3547)
                     /locus_tag="PKK36_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94111.1"
                     /translation="MISFPPEWKSIKAVLSHDWLTGMRGGERVLELLCEGFPEAPVYT
                     LLHNPGAVCETISRHRIETSCLQKVPGIFSKYRWFLPFFPWVVGRMKTPGADVLISTS
                     HCIAKGVKPAPGTRHLCYCFTPMRYAWIFYDEYFGKNPFKKALFKPVLNRLRQWDMKA
                     SERVDHFVAISEHVRRRIQDCYGRDADVVYPPVNTDFYTPAGEPEDFDLIVSALVPYK
                     RIDLAVTACSRMGSKLKIVGIGTNDAALRRAAGPNIEFLGWQDDAAVRDLYRRCRCLV
                     FPGEEDFGIVPLEAQACGRPVVAYARGGALETVVDGVSGVFFKNQTADDLIAALEKCH
                     QTTWNSSAIRAHAEKFSRQHFIDGFAESIRTTLAT"
     gene            complement(3595..4017)
                     /locus_tag="PKK36_05820"
     CDS             complement(3595..4017)
                     /locus_tag="PKK36_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR94112.1"
                     /translation="MEHKEISEIIIGCAYKVFNEMGFGFLESVYEKCLMIEFKKAGLK
                     AENQQPIRVLYKGHLVGEFFADIIVEDKIVVELKSVSRLIKAHEVQLVNYLTATGLPV
                     GLIINFAESGVEVKRKVRQLNKHAVAMISNPVNPVDPV"
     gene            complement(4048..5190)
                     /locus_tag="PKK36_05825"
     CDS             complement(4048..5190)
                     /locus_tag="PKK36_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR94113.1"
                     /translation="MRIGIDARWIFEKISGIGSYTQELIAHLPEVDQQNHYVLLFGSR
                     TVMERTSNLARLEERRNVETVLFEYSPFSWRSQTLLPRRLRKLNLDVYHAPNYMIPFP
                     AFAKDASRKTACVMTIHDLIPLIFPDHAPKSKKSRLFPLFKKIMLAAAARADLIIAPS
                     ECSRNDIIARLQIPETKRSRVVVIPEGVDARYKPLPQPSPSKVVLYVGRQDPYKNLAS
                     LVRAFAKVHEAVPSAVLRVIGPPDKRYPEAFELARELNILPHIDWHGYVDNAGLLAAY
                     QQAAVLVLPSRYEGFGLPVVEAMACGTPVVCSNVSSLPEVAGDAALMADPGDASAFAE
                     HIVNVLTRPELARGLREKGLARAAQFTWQKTAEQTLKAYEEAKRNI"
     gene            complement(5233..6339)
                     /locus_tag="PKK36_05830"
     CDS             complement(5233..6339)
                     /locus_tag="PKK36_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR94114.1"
                     /translation="MDIQAAIAQRAGVGRYARMLVEHLAPLAGKDELALFYFDFKRSG
                     VPFPVPGAEQQAVQWCPGRIVQKAWKTMGWPPFDWFAGRADIYHFPNFILPPLTRGRT
                     AVTIHDVAFLRHPEAAEKRNLKYLNSRIADTVKRADAILTDSHFSAREITELLKVPPE
                     KLYPVHLGLSPDMKPASQEDIEKVRNLYGLQKPFLLTVGTLEPRKNIPFLIDVFERLA
                     DFDGDLVIAGMKGWSYEPILARMRASKQAERIRYLEYVEDGLLSALYSGAELFVFPTI
                     YEGFGFPPLEAMACGTPVVSSNAGPLPELLGSAAALVDGYLAGEWVEMLSGVLRDELR
                     RNALRAKGLEQSAKFDWNKTAAETWKIYRKIIRG"
     gene            6424..6612
                     /locus_tag="PKK36_05835"
     CDS             6424..6612
                     /locus_tag="PKK36_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94115.1"
                     /translation="MQKQFDQLRASELFCPRCRTSRPVRETLLLVVPGGEIHSYRCKI
                     CGEHLGTREVKGRPGNIG"
     regulatory      6814..6988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7019..>7395
                     /locus_tag="PKK36_05840"
     CDS             7019..>7395
                     /locus_tag="PKK36_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94116.1"
                     /translation="MKRMVCVAVCAVFAAAITARAGWITLLSDSFDGSTISQWSYQGA
                     TNTSGQSLFRCFNIHELIQAEWDQSNFFSGLSDPYLILPSSLSRSLGRTLTDRDTFRI
                     SATLNITPGSVPDTTEFYQIANFG"
     assembly_gap    7396..7495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7496..7759
                     /locus_tag="PKK36_05845"
     CDS             <7496..7759
                     /locus_tag="PKK36_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94117.1"
                     /translation="ENGFGQGTYDDLRVEQYFAPGEFFAEEMNSDGFRISFASESGTV
                     YQVESSSDLHSGWSPAAAVTSVAETVTFTNSMPDTVRFYRVTK"
     gene            7793..7939
                     /locus_tag="PKK36_05850"
     CDS             7793..7939
                     /locus_tag="PKK36_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94118.1"
                     /translation="MGTQAERCCGAPGGLAPPERKGQINGNRTAPFPDPIHLRDKPAG
                     VRTG"
     gene            7969..8700
                     /locus_tag="PKK36_05855"
     CDS             7969..8700
                     /locus_tag="PKK36_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94119.1"
                     /translation="MIGVLAGLIAMLLPMLTQGREAGRSVVCQSNLRQMQLAYAAYLM
                     DHKGMFFPFRENTSEGTLWYFGLEKGSGPEGSRKIDMTKARLAPYFEQSGGIAVCPSL
                     AKRSRHFKQKFSMATYGYGLNIFMLLGTPQQKKAGVSTFEEVEDPSETITWADCAQIN
                     TWQPPASPGNPMLEEWYWLAPCIPGAPAPPPFHFRHGGILNAAFADGSVRTFLPDKTD
                     PRCDGLTGYIEPCGEDAWLRPLKTR"
     gene            complement(8707..11121)
                     /locus_tag="PKK36_05860"
     CDS             complement(8707..11121)
                     /locus_tag="PKK36_05860"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNR94120.1"
                     /translation="MDTAAGCLVAGKYAFTELIAADRLRELFESFVRVSGLAARLVSL
                     PDHEIIASSGWSDICSRFHRSNTGSEALCLANSRRLAVALNGPLEPSICVCENGLVAA
                     AEAVWVKGVHVANLYAGQILFEKPDIARFKKQAEAFSYDVNAYLESLSKVPVVPESSF
                     REALSFLGHMVVLHAEQGYAELQARENAEKFRSLAENAVDYIFIKDRSRRYTFVNRAF
                     QQLLGRSESEILGKTPLEIYGPESARIVEEVDDRTFAGESVDETRKLMVGGREYYFQT
                     AQAPLARKDGEVISIMGIVHNVTTLHLAEESLRENNARTAAMLAAMPEMVFVLAADGT
                     YMDFKADREDELAIPPREFIGKKIGEAMFDEDQVRFILEQIQITLKTGRVHSFEYKLR
                     RRAFDEYFDARLAPFGPDKVLATVRNITYRKNVEEELQTMERLKSIGVLAGGLAHDFN
                     NILMGLYGNLALAREMLPSGSPACAPLEEAEKSMERATGLTRQLLTFAKGGAPVREHV
                     RLDNLVKNISAFNLAGSSVKLVFEEVQNLWCAEVDRSQMQQVFSNLIINAVQAMPNGG
                     NLFVGLLNLEVGPDDIPGLTAGKYVKISLRDEGVGIGKKDMARIFDPFYTTKQTGTGL
                     GLATAYSIVSRHGGHISVESELRKGTVFDIYLPVSSAQEPEKTLSASDQKGAAWRAAR
                     VLVMDDDPAICRIVVRMLQEPSREVEVAPDGQKAIELCRTAVADGQPYDVVIMDLTIP
                     GGMGGMDAIGPVREIIPGIKAIVSSGYADDAVMSNFTEYGFNGVIAKPYTKPRLLQVL
                     DQVMRE"
     gene            complement(11191..12621)
                     /locus_tag="PKK36_05865"
     CDS             complement(11191..12621)
                     /locus_tag="PKK36_05865"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNR94121.1"
                     /translation="MKKDQAQVLMEKLVSLCKRRGFIFQSSEIYGGINGFWDYGPLGV
                     ELKRNIRDAWWKAMVRDREDVVGLDASIIMHPRVWEASGHLTGFTDPMVDCKACKKRY
                     RADQLCEELGITIETGSAEEHGFKLPPGTQCPACGSTDLTEPRDFNLMFKTFIGPVED
                     ASSVAYLRPETAQAIFAQFNNVLSVSRQKVPFGIAQIGKAFRNEINPRNYTFRSREFE
                     QMELEFFIKPGTDAEWHEYWVAERLRWYETIGLPSSSLHRDIHPKEKLAHYASACTDV
                     AYEFPFGIQELEGIAARGNFDLSRHQEFSGKSMEYFDQETNEKYVPHVVEPSAGVDRI
                     ALALLCNAYAEEWVPTKDTGGEVLAADITASRPPEGYEMRTVMRFAPCIAPVKTAVFP
                     LLKNKPELVAKAREVFEIAKACGPAFYDEGGAIGRRYRRQDEIGTPYGVTIDFDTLKD
                     ETVTLRDRDTMKQERVKIAELPALLK"
     gene            12724..14391
                     /locus_tag="PKK36_05870"
     CDS             12724..14391
                     /locus_tag="PKK36_05870"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNR94122.1"
                     /translation="MIMYRLIIEEPGGRKRSVAVQEGAVALGRDPSCDVQLMDDEVSW
                     KHAMIETGPDGLRIRDMGSLNKITLNGADVREARLADGDRIELGRTRILFQDARAAVQ
                     PAGRRVSLLHRITLTAVALVLLIELVLVAALFIWRHTGGDELLMAESFDTAEIPEKPV
                     EPPKKPVEVAKVEAKPATVSAGRMPETPEPKPDPVPDRLTDVEWITPPVAEAVPEKPE
                     VPAPAEEPEPEPDTHVELESEPAPAVPDRDEEIEPAAEPLEPASAPDKEIVPEPEPVP
                     ETIPEPVKEKSEEEIQTPAEKPESVEPAAPEDPLITLARNMLREARTDIAKMNYTEAD
                     SRLERIEIVAPDYLPAYEERAKLYESRGMLQQAGEQWAEVMRRSQGKPDYARAAAERS
                     RLAQKTSAPGVITAPRRIAALSAPDKPLPKRVMIQSVDMDEFGPGPEFDEMRLLRIRL
                     RPKASEKNIEGGEVAVNVSFYERDRRTRAVQPAEAYVPGSIVLPGGIWSSGGIYEAQA
                     TYLVSRALGSTGSDMSYYGYVIQVFYQDVLQDERISPPALKDYLTGN"
     gene            complement(14388..14999)
                     /gene="trmB"
                     /locus_tag="PKK36_05875"
     CDS             complement(14388..14999)
                     /gene="trmB"
                     /locus_tag="PKK36_05875"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNR94123.1"
                     /translation="MEQTRRYVPENWLEPFRLADLFAGPAPFEVDVGCGKGRFLLARA
                     AAHPEINYLGVDRMLRRIRKVDRKAVRAGLDNVRLLRMDACYAVTWLIPSNCVSAYYV
                     FFPDPWPKTKHHHHRLFDRRFMDALVRTLIPGGIVNFATDHLPYYDEVRGLLEADGRF
                     EPAEAFVPCEEEKTDFELMFIGQKPIGRCSFRAAARGGAEASL"
BASE COUNT         3263 a         4478 c         4326 g         3026 t
ORIGIN      
        1 tggatcttcc gcgaaatcaa atcgctctgg gagagcggcg aggcctcccc tcccccctcc
       61 ctgcaggagc ggcgcgccat gatcgaacgg catctcaagc tgtcggttga acacgcgctg
      121 attgagaaca agtaccgcaa gcgccgccgg aacacggccg agcaggcggc ctgctgctat
      181 tttcgcgccc atctggtgcg ctatctcgcg gggcttaacg gataccgtca tatcctcctc
      241 cggatggaat gcatgaattc cgtcgaggaa atcatgaagg aagtggatct gctgattgaa
      301 cgcaacctca acctggaaat ggaacatgga agaaacgaag aatcttgagc atggaacgca
      361 gccgcttgat gcgctgctga acgggctgga aatcgcaaac ggcgatctcg tgcgggcctc
      421 caccgaacac ctcacgttca aagtggtgca gaagggccgg cggggccggg aactcaccat
      481 gcgcgcgcag ctcaagatcg tcaacgcgct caaccgtctt cggccggacg atcccgtcag
      541 gccggcggac ctgttcaact accgggggcg ctgattccgg tttgcatttc aggccggaga
      601 acggtatttc tgaaccatgg agcttgaaac atatgtcgga cacaactctt tctgaacttc
      661 tctggagata tgatcccagc ggcattgtcg tggcggatca ggacatgatc atccggcagg
      721 tgaatcccgc cttctgccgc atcttccggg tggatgcgga caccatcacc gggcagccgc
      781 tgtcgcgggc gctgcgcgaa gagccgcgct atttcatcag cgcctggaac gacaaccggc
      841 ccctgcgcgg ccacgacaag ctctacatgg acaacaacgc ctatgtccgt cacctgattg
      901 tgccgctgaa ggaaaacggg ctggtggtcg gcattctgac cgacatgacg gaggagtggc
      961 ggcgccggca gatgctggag accgcgcgga agcagatcat ggaagagcag caggcgctcg
     1021 gccatccgga cgggagccgc ctcccggcga ttctcaaaac ggtggaagaa cttctggctt
     1081 gaacgctggc tccggcggca gggctcgaac ctgcaaccta gtggttaaca gccacccgct
     1141 ctaccattga gctacgccgg aaccgtcaag gattcgaaca cgataatatc caacttgtgg
     1201 aagtcaagga ttcttgaaaa aaaacggaag cgctcagggc atccacgtgc gcgccagcgt
     1261 ccatatcccc agcaccgtga tcaggccgcc catcatgtaa tggatggaag cctgcttcag
     1321 gcttcgcgtg gtgaaggccc cgaacggcgc caccatgatg gatccgatga acagcgccag
     1381 gatcagttcc cactggaaaa gctgcgggga aaacatccgc gtgaagaaat ccgccagtcg
     1441 catttcaagc accgctcccg gcacctcacg gaccgtgcgg aagatcatat aggcaaagaa
     1501 accggaaatg cagattggcg cctgcgccat ggtggtgatg cccacggcgg ctttatgctc
     1561 ctgaccggcc atgacctgtc cgcccgtaat caccgggcca aacccgccgc ccgtcatgcc
     1621 cttgttgaac gcgctcagga ttcccacgaa gaccatccgg ttccaggtga aggtgaaact
     1681 cttccgggtc agcaggatga ttcccatggc ggtcaccagc aggccgatgt aggtcttcag
     1741 aacaaccttc ggaatgttca cggcaatcac cgccgccatg acggccgcca caatgccaaa
     1801 ccccaccacc atgatcagca tcttccattc acgcgaactg ggctcgaacg aaacattctc
     1861 aaactgctga tggaacatcg aggccacaaa accttcaaaa gcctgcgtga gaagaatggc
     1921 cggaaccacc accgtcgggc tgaagcccat gatcagcagc agcggaccaa ggattgtgcc
     1981 gtagcccatg cccatggacg aatctataaa cgcgcataca aacgccatgg ccatgagcac
     2041 aagaaaaatg cctaccgtga tttccatagg ccccgtatcc tagcagaagc cgcaggccgc
     2101 accagtgaaa tcagcggccc cgggaaaaag ttccagtcat tggaagttct gcgcggaatt
     2161 tttcggtcct gggggcgttt tgagaccttt ttaggcgttt tgggcctttt tttcgtattt
     2221 tcagacgttt tgagggcttt ttgagagatt tttcgatgtt tgcgccgatt tccggagctc
     2281 attgcactgc aaacagataa gcagattttt ttcggaaaat caagcaaaat cggtcgtttt
     2341 tcgcggattt tggccatttt ttggcgattt tcaacggttt ttgaccattt acgagccttt
     2401 tttgggcatt ttttgcgatt tttcgcgctg gattagctca agttgccagt gtcgtccgga
     2461 tgctttcggc aaaaccgtca atgaagtgct gccggctgaa tttttcggcg tgggcccgga
     2521 ttgcggagga attccatgtc gtctggtggc atttttcgag ggcggcaatc agatcgtcgg
     2581 cggtctggtt tttaaagaag accccggaga cgccgtccac aaccgtttcg agcgctcccc
     2641 cgcgcgcata agcgactacc ggccggccgc aggcctgcgc ctcgagcggg acgatcccga
     2701 aatcctcttc gccggggaac accagacagc ggcagcgccg gtaaagatcg cgcaccgccg
     2761 catcatcctg ccagccgaga aattcaatgt tcggcccggc ggcgcggcgc agcgcggcgt
     2821 cgttggtgcc aatgccgacg attttgagtt ttgatcccat ccggctgcag gccgtgacag
     2881 cgaggtcaat tctcttgtac gggaccagcg cggacacgat cagatcaaaa tcttccggct
     2941 cgccggcagg ggtgtagaaa tccgtgttca cggggggata gaccacatcg gcatcgcgcc
     3001 cgtagcagtc ctggatccgg cggcggacat gctcgctgat ggccacgaaa tgatccaccc
     3061 gctcggaggc cttcatatcc cactgccgga gccggttcag aaccggcttg aacagggctt
     3121 tcttgaacgg gttcttcccg aaatactcgt catagaaaat ccacgcatag cgcatgggcg
     3181 tgaagcagta gcagagatgc cttgttccgg gcgcgggctt cacgcctttg gcgatgcagt
     3241 ggctggtgct gatgagaaca tccgccccgg gggttttcat gcggccgact acccagggga
     3301 aaaacggaag aaaccagcgg tacttcgaaa agatgcccgg cactttctgc aggcacgatg
     3361 tttcgatgcg atggcggctg atggtttcgc aaacggcgcc cggattgtga aggagcgtat
     3421 aaaccggggc ttcggggaac ccctcgcaca gcagttcaag cacgcgctcg ccgccgcgca
     3481 tgccggtgag ccagtcgtgc gaaagcacgg cctttatgct tttccattct ggaggaaacg
     3541 aaatcattgc gtctctttat gctctgttac agcctcagcc atttttcttc atttttagac
     3601 aggatcaaca ggatttacgg gattggagat catagcaacc gcatgcttgt tgagctgcct
     3661 caccttgcgt ttaacttcaa ctccgctttc tgcaaagttg attattaggc ccaccggaag
     3721 acccgttgcc gttagatagt tgaccaattg cacttcatgc gccttgatta gcctgctcac
     3781 agacttgagt tcaacgacaa tcttgtcttc aacgatgata tcggcgaaaa attcgccaac
     3841 aagatgcccc ttgtaaagca cccgaattgg ctgttgattt tcggccttta acccagcctt
     3901 tttgaactca atcatcaggc atttctcata gacagattca agaaacccaa agcccatttc
     3961 attgaacacc ttgtaagcgc atccgatgat tatttcggat atttctttgt gttccattct
     4021 gattcctgtt gatcctgtaa atcctgtcta aatatttctc tttgcttcct cgtacgcctt
     4081 cagggtctgt tcggcggtct tctgccatgt gaactgcgcg gcgcgggcga ggcctttctc
     4141 gcgcaggccg cgggccagct ccggacgggt cagcacattc acaatatgtt ccgcaaaggc
     4201 gctggcgtcg ccaggatcgg ccatcagcgc ggcatcgccc gcgacttcag gaagcgagct
     4261 gacattgctg cagacgaccg gcgtcccgca ggccatggcc tcgaccaccg gcaggccgaa
     4321 gccctcgtac cggctgggaa gcaccagcac ggcggcctgc tgataggcgg ccagcaggcc
     4381 ggcattgtcc acatagccgt gccagtcaat gtgcggaaga atattcagct cgcgcgcgag
     4441 ttcaaatgcc tccggatagc gtttgtcggg cggaccaata acgcgcagca ccgcggaggg
     4501 gacggcttca tgcactttgg caaaggcccg aacgagcgag gccaggttct tgtagggatc
     4561 ctgacggccc acatagagaa ccacctttga aggagacggc tgcggaaggg gcttgtagcg
     4621 cgcgtccacc ccttccggaa tcaccaccac gcggctgcgc ttcgtttccg gaatttgcag
     4681 ccgcgcgatg atgtcattcc gcgaacactc gctgggcgcg atgatcagat cggcgcgcgc
     4741 ggcagccgcc agcataatct ttttgaacag cgggaacagg cggctctttt tggatttcgg
     4801 agcatggtcg ggaaagatca gcgggatcag atcgtgaatc gtcatcacgc acgcggtttt
     4861 gcgcgaggca tccttcgcaa aagccggaaa cgggatcatg tagttgggcg cgtgatagac
     4921 gtcgagattc agcttgcgca ggcggcgggg aagaagcgtc tgcgaccgcc aggaaaaggg
     4981 ggaatactca aacagcacgg tttcgacatt ccggcgttcc tccagccgcg cgaggtttga
     5041 cgtgcgctcc atcaccgtgc ggctgccgaa gagcaggaca tagtgattct gctggtccac
     5101 ctcgggaaga tgagcaatca gttcctgcgt ataggagccg atcccgctga tcttctcaaa
     5161 aatccatctg gcgtctattc caattctcat tttcaagccg ttctaaccac ggaccttcaa
     5221 tatttaagag ttttaaccac ggattatctt cctgtagatt ttccaggttt cggcggcggt
     5281 tttgttccag tcgaattttg ccgactgctc gagccccttc gcgcgcagcg cgtttcttct
     5341 gagctcgtca cggagaacgc ccgagagcat ttcaacccat tcgcccgcaa gatatccgtc
     5401 caccagcgcg gcggcggaac ccagcagttc cgggagcggg ccggcattgg acgacaccac
     5461 cggtgtgccg caggccatcg cctcgagcgg ggggaatccg aaaccctcgt aaatcgtcgg
     5521 aaagacgaag agctctgcgc ccgaatacag cgcgctgagc aggccgtctt cgacatactc
     5581 cagatagcgg atgcgttccg cctgttttga tgcccgcatc cgcgcgagga tcggctcata
     5641 gctccagccc ttcatgccgg caatcaccag atcgccgtca aaatccgcca gccgctcaaa
     5701 cacatcaatc aggaacggaa tgttcttgcg cggctccagc gtgccgacag tcagcagaaa
     5761 gggtttttga agcccgtaga ggttccgcac cttttcgatg tcctcctgcg acgccggctt
     5821 catgtcgggc gagagcccga gatgcacggg atagagtttc tccggcggga ccttcagcag
     5881 ttccgtgatt tcacgcgcgg aaaaatgaga gtcggtcaaa atcgcgtccg cccgcttcac
     5941 ggtgtcggca atgcggctgt tcagatattt caggttccgc ttttcggcgg cctcaggatg
     6001 ccggagaaag gcgacatcgt gaatggtcac ggcggttctt ccccgcgtga gcggcggcag
     6061 gataaaattg gggaaatgat agatatccgc gcggcccgca aaccagtcga acggcggcca
     6121 gcccattgtc ttccaggcct tctgcacaat gcggccggga caccactgaa ccgcctgctg
     6181 ctccgcgccg ggcaccggga aaggaactcc gcttcgcttg aagtcgaaat aaaaaagggc
     6241 cagttcatct ttgcccgcga gcggggccag atgctccaca agcatgcggg cataacgccc
     6301 aacgcccgcc cgctgcgcga tcgccgcctg aatgtccacg caaactttca atccgaatcc
     6361 tttttaccga ttgacgcatt gtaagcgtcc gccatactcc agcaagtgat taattgatgc
     6421 gccatgcaaa aacagtttga tcagttgagg gccagcgagc ttttctgccc gcgttgccgg
     6481 acctcacggc cggtgcggga gacgcttctg ctggtcgtgc ccggcggcga aattcacagc
     6541 taccggtgca agatctgcgg cgagcatctc ggcacgcgcg aggtcaaggg ccggccgggt
     6601 aatattgggt agggcgcgca ctccgttgcg cgccgcggcg gccgcggagt ggccgccctg
     6661 ccccagaatc ttcccggcgc ccccggcggg cttgccccgc cggagcgcaa aggtcacacc
     6721 aaaccggcac gcaactccag ccaaccccaa cctcattcac ccgcgacaca aggtcgcggg
     6781 ggtcttgaca gaacccctcc gcttctctac cgtcactcgc cgttaactga aagtcatgac
     6841 cgcaaacgga agcccgtgag aatcgggcgc ggtcgcgccg ctgtaaccgg tgttcagttc
     6901 ccaaatgcca tttccgcccc ggcggagaga aggcgggagc cgacagaagc cggaagccag
     6961 aagacgagcc gtcatgacat aacccgaacc cccgcgcaat ggggtgaaag gaaatgaaat
     7021 gaagcgcatg gtctgcgttg cggtttgtgc tgtttttgcc gccgcgatta ccgccagggc
     7081 cggatggata accctgctga gtgattcgtt cgacgggagc acaatttccc agtggtccta
     7141 tcagggcgca acgaacacct cggggcagtc tttgttccgc tgcttcaaca tccatgagct
     7201 gattcaggcg gaatgggacc agtccaattt tttttcagga ctttcagatc cctatctcat
     7261 cctcccctcc tccctgtcac ggagcctcgg ccggaccctc acggaccggg acacgttcag
     7321 gatcagcgcc acgctgaaca tcacccccgg gtccgttccc gacaccactg aattctatca
     7381 gatcgcgaat ttcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaaa
     7501 cggctttgga caggggacgt atgatgatct tcgggttgaa cagtattttg cgcccggaga
     7561 attcttcgcc gaggaaatga attctgacgg attccgcatt tcctttgcct cggaatccgg
     7621 aacagtgtat caggttgaat cctcttctga cctgcattcc ggctggagcc ccgcagccgc
     7681 ggtcacctcc gtcgcagaaa ccgtcacatt cacgaacagc atgccggata cggtgcggtt
     7741 ttacagagtg acaaaatgaa aacagaggcc actgaaaaca gcaaagcgtc gcatgggcac
     7801 gcaggcggag aggtgctgcg gcgcccccgg cgggctggcc ccgccggagc gcaaaggcca
     7861 gatcaacggg aaccgcaccg ctccttttcc cgacccgatt cacctgcggg acaagcccgc
     7921 aggggtccga acaggataag cgcattcacg atcattgaac tgctcgccgt gatcggggtt
     7981 ctggcgggtc tgatcgcgat gcttctgccg atgctgacac agggccggga agcgggccgg
     8041 tcggtggtct gccagtcgaa tctgcggcag atgcagctgg cctacgcggc ctatctgatg
     8101 gatcacaaag gaatgttctt cccgttcaga gagaacacct ccgaggggac gctctggtac
     8161 ttcggactgg aaaaagggag cgggccggag ggctcccgca aaattgacat gaccaaggca
     8221 cggctggccc cttatttcga gcagtccggc gggattgcgg tctgcccgtc gctggcaaaa
     8281 cggtcacggc acttcaaaca aaaattcagc atggccactt acggctacgg gctgaatatt
     8341 ttcatgctgc tgggaactcc ccagcagaag aaagcgggag tcagcacatt tgaagaggtg
     8401 gaagatccgt cggagacgat cacatgggcg gactgcgcgc agatcaacac ctggcagccg
     8461 cccgcctcgc cggggaaccc gatgctggag gaatggtact ggctcgcgcc ctgcattccc
     8521 ggcgcccccg ccccgccgcc ttttcatttc cgtcacggag gaatactcaa tgcggccttt
     8581 gccgacggca gcgtgagaac ctttctgccg gacaagaccg atccgcgatg tgatggcctg
     8641 acgggctaca tcgaaccgtg cggagaagat gcctggctgc ggccgctgaa gacgcgctag
     8701 ccgaaactat tcccgcatga cctggtccag gacctgcagc agcctgggtt ttgtgtaggg
     8761 cttggcaata acgccattga acccgtattc tgtgaaattc gacatgaccg cgtcatcggc
     8821 atagccgctg gaaacaatgg cttttatgcc cgggattatc tcacggacag ggccaattgc
     8881 atccatgccg cccattcctc cgggaatggt caaatccatg atgaccacat cataaggctg
     8941 tccgtccgcc acagctgtgc ggcagagttc aatggctttc tggccgtcgg gcgcgacttc
     9001 cacctcccgg ctcggttcct gcagcatgcg cacaacgatt ctgcagatcg caggatcatc
     9061 atccatgacc agaactcgcg ccgcccgcca tgccgcacct ttctggtcgg aggcgctgag
     9121 tgtcttttcc ggctcttgcg cggatgacac gggcagatag atatcaaaca ctgtgccctt
     9181 tcgcagttcc gactcaacgc tgatatgccc cccatgcctg ctgacaatgg aatatgcggt
     9241 ggcgagaccc aggcctgtgc ccgtctgttt tgtggtgtaa aacggatcaa aaatccgggc
     9301 catatccttt ttccctatgc ccacgccttc gtcacgcagg gagattttta catatttccc
     9361 ggccgtcagc ccgggaatat cgtccggtcc gacctcaaga ttcaacaggc cgacaaacag
     9421 attgccgcca ttcggcatgg cctgaacggc attgatgatc aggttggaga agacctgctg
     9481 catctggctt ctgtccacct cggcgcacca caggttctgc acctcttcaa atacaagctt
     9541 gaccgaactc ccggccagat tgaacgcgga aatattttta accagattgt ccaggcgaac
     9601 gtgctcccgg accggggccc ctcccttggc gaaggtgagc agctgccggg tgagaccggt
     9661 cgccctttcc atggattttt ccgcctcctc aagaggtgcg caggcaggac tgccggaggg
     9721 aagcatttcc cgggcaagag caagattgcc gtacagaccc atcagaatat tgttgaaatc
     9781 atgcgcaagg ccgccggcga gaaccccaat gctctttaac cgctccatgg tctgcagttc
     9841 ctcttcaaca ttcttcctgt atgtgatgtt ccgcaccgtg gccaggacct tgtccgggcc
     9901 gaacggcgcc aggcgggcat cgaagtattc atcgaaagca cggcgcctga gtttgtactc
     9961 gaagctgtga acacggcctg tcttcaatgt gatctgtatc tgttcaagaa taaagcgaac
    10021 ctgatcctca tcgaacatgg cctcaccgat ttttttacct ataaattcac gaggcggaat
    10081 ggccagttca tcctcccgat ccgccttgaa atccatgtac gtgccgtctg ccgccagaac
    10141 aaacaccatt tcaggcatgg ccgccagcat ggcggcggtt cgagcattgt tctcccggag
    10201 cgattcttcc gccaaatgca gcgttgtaac gttgtgcaca atgcccatga tcgaaatgac
    10261 ttcaccgtct tttcttgcca gcggagcctg ggctgtctga aaataatact cacggccgcc
    10321 caccatgagt tttctggttt catcaactga ttccccggcg aacgtgcggt catccacttc
    10381 ttcaacaatg cgggcactct ccggcccgta gatttccagc ggtgttttcc ccagaatctc
    10441 gctttctgac ctgcccagca gctgctggaa ggcccggttc acaaatgtgt atctgcggga
    10501 cctgtcctta atgaagatat aatcaacggc gttttcggcc agggatcgga atttctccgc
    10561 gttttcccgg gcctgcagtt cggcataacc ctgttcggca tgcagaacca ccatgtgccc
    10621 aagaaagctc agcgcttcgc ggaaggagga ttccggaacc accgggacct tgctcagcga
    10681 ctcaagatag gcgttgacat cataactgaa ggcctccgcc tgtttcttaa agcgcgcaat
    10741 gtcaggtttc tcaaacagga tctgccccgc atagagattc gccacatgaa cgcctttgac
    10801 ccagacagcc tcagcggcgg ccacaagccc gttttcgcaa acacatatgc tgggttccag
    10861 cggcccgttc agcgcgactg ccagcctccg gctgtttgcc aggcagagag cttcagagcc
    10921 tgtattggag cggtgaaacc gggagcagat atcgctccag ccggaggacg caattatttc
    10981 atgatcaggc agggaaacca gtctcgccgc aagcccggaa acccgaacaa aactttcgaa
    11041 cagttcccgc agccggtccg ctgcaatcag ctcagtgaat gcatacttcc cggcaacaag
    11101 acagcctgca gcggtatcca tatccctccc cctgacaagc agtatagcaa acttcccggg
    11161 aaagggaata tggaaaaata ttttttgaga ctatttcagc agggccggga gttccgcaat
    11221 cttcacgcgc tcctgcttca tggtgtcgcg gtcgcgaagg gtgacggttt catccttgag
    11281 ggtgtcgaag tcaatggtaa ccccgtaggg cgtgccgatt tcatcctgcc ggcggtagcg
    11341 ccggccgatg gcgccgccct cgtcgtagaa agccggaccg caggccttgg cgatttcgaa
    11401 aacctcgcgg gcttttgcga ccagctccgg cttgttcttg agaagcggga aaaccgcagt
    11461 cttgaccggc gcaatgcacg gggcaaaacg catgacggtg cgcatctcgt agccctccgg
    11521 cgggcgcgaa gcggtgatgt ccgccgcgag cacttcgccg ccggtatcct tggtgggaac
    11581 ccattcttcg gcataggcgt tgcagagcag agccagcgca atgcggtcca ccccggccga
    11641 gggttcgaca acatgcggga cgtatttctc gttcgtctcc tggtcgaaat attccatgga
    11701 cttcccgctg aattcctgat ggcggctcag atcaaaattg ccgcgcgcgg caatgccctc
    11761 aagctcctgg atgccaaacg ggaattcata tgcgacgtcg gtgcaggcgc tggcgtagtg
    11821 cgccagcttc tctttcggat gaatgtcgcg gtgcagactg gaggacggca ggccaatggt
    11881 ttcataccag cgcaggcgct ccgccaccca atactcgtgc cattcggcat cggttccggg
    11941 cttgatgaaa aattccagct ccatctgctc gaactcgcgc gagcggaagg tgtagttgcg
    12001 gggattgatt tcattgcgga aggccttgcc aatctgggcg atgccgaaag gcaccttctg
    12061 gcgcgacacg gagagcacat tgttgaactg cgcaaagatc gcctgggcag tctcgggccg
    12121 cagataggcc accgaggagg catcctccac cggtccgatg aaggtcttga acatcaggtt
    12181 gaaatcacgg ggctcggtca gatcggttga gccgcaggcc gggcattggg tgccgggggg
    12241 aagcttgaac ccgtgttcct cggcgcttcc ggtttcaatg gtgatgccca gttcctcgca
    12301 gagctggtcg gcgcgatagc gctttttgca ggctttgcag tccaccatgg gatcggtgaa
    12361 tccggtgaga tgacccgagg cctcccacac gcggggatgc atgatgatgg aggcatcaag
    12421 tcccaccacg tcctcacggt cgcgaaccat ggctttccac cacgcgtcgc ggatgttgcg
    12481 cttgagttcc acgccaaggg gaccgtaatc ccagaaaccg ttgatgccgc catagatttc
    12541 cgagctctgg aagataaagc cgcgccgctt gcagagggaa accagtttct ccattaaaac
    12601 ctgtgcctga tcctttttca tagcgcgcgg atggtgcaga agttcatcgg cggggtcaag
    12661 caaagaacgc gcggacgcat tctgtgcttg tcgcgcggcc caaggtttat ttcactggac
    12721 ggcatgatta tgtacagact catcatcgaa gagcccggcg gccgcaaacg aagcgttgct
    12781 gttcaggaag gcgccgtggc actcggccgc gatccatcct gcgatgtgca gctgatggat
    12841 gatgaggttt cgtggaagca tgccatgatc gaaacggggc cggatggtct gcgcatccgc
    12901 gacatgggct cgctcaacaa gatcacgctg aatggagccg atgtgcgcga agcgcgtctg
    12961 gcagacgggg accggattga actgggacgc acccgaatac tttttcagga cgcacgcgcg
    13021 gccgtccagc ctgcgggaag gcgggtcagc ctgctgcacc gcatcaccct gacagcagtg
    13081 gcgctggtgc ttctcattga actcgtgctg gtcgcggcac tgttcatctg gcggcacacc
    13141 ggcggagatg aactgctgat ggcggagagt tttgacaccg cggagatccc cgaaaaaccc
    13201 gtggaaccgc cgaagaaacc cgttgaagtt gcgaaggttg aagcgaagcc cgcaacggtc
    13261 agcgcgggca ggatgcctga gacgcccgag ccgaagccgg accctgtgcc ggaccgcctg
    13321 accgatgtgg aatggataac cccgcccgtg gcggaggccg tcccggagaa gcccgaagtc
    13381 cctgcgccgg ccgaagaacc ggaaccggaa ccggacacac atgtggaact ggaatccgaa
    13441 cctgcgcccg ctgtgccgga tcgggatgaa gaaatagagc cggcggcgga acctctggag
    13501 cccgcatcag caccagacaa agagattgtg ccggaaccag aaccggttcc cgaaacgatc
    13561 ccggaacccg taaaggaaaa atcggaagaa gaaatccaaa ccccggccga gaaacccgaa
    13621 tccgttgagc cggccgcccc cgaggatccg ctgatcacgc tggcccgcaa tatgcttcgg
    13681 gaggcccgca cggatatcgc aaagatgaac tatacggagg cggacagccg cctcgagcgg
    13741 attgagattg tggcgccgga ttacctgccc gcctacgaag agcgcgcgaa gctgtacgaa
    13801 agccgcggca tgcttcagca ggccggcgaa cagtgggctg aagtcatgag gcggtcgcag
    13861 gggaaaccag actatgccag ggcggcggcc gagcgcagcc ggctggcgca gaaaacctcc
    13921 gctccggggg tcattaccgc gccccgccgg atcgcggccc tttccgcacc ggacaaaccc
    13981 ctgcccaaac gggtaatgat tcagtccgtg gatatggatg aattcgggcc ggggccggag
    14041 tttgacgaaa tgcgcctgct ccgcatccgt ctgcggccga aggccagcga gaaaaacata
    14101 gagggcggcg aggtggccgt gaatgtgtcc ttctatgaac gagaccgccg cacacgcgcc
    14161 gttcagccgg cggaggccta tgtgcccgga agcattgtac tgcccggagg gatctggtcg
    14221 tcaggcggaa tctacgaggc ccaggccacc tacctggtct cccgggccct gggatcaacc
    14281 ggttctgaca tgtcatacta cggatatgtc atccaggtgt tctatcagga tgtccttcag
    14341 gatgaacgga tctcgccgcc ggctctgaaa gattatttaa ccgggaatta aagcgaggcc
    14401 tctgcgcccc cgcgtgcggc cgcccggaaa gagcatcgcc cgatcggttt ctgcccgatg
    14461 aacatcagct cgaaatccgt tttttcctcc tcgcacggga caaacgcctc ggccggttca
    14521 aagcggccgt ccgcctccag cagaccgcgc acttcatcgt aatacggaag atgatccgtg
    14581 gcgaaattca caatccctcc ggggatcagt gtgcgcacca gggcgtccat gaaccgccgg
    14641 tcaaaaagcc ggtgatgatg gtgcttcgtc ttgggccagg gatccggaaa aaaaacataa
    14701 taggcgctga cacagtttga tgggatcagc caggtcaccg catagcaggc gtccatgcgg
    14761 agcaggcgca cattgtccag gccggcgcgc accgccttgc ggtccacttt gcgtatcctg
    14821 cgcagcatgc ggtccacgcc aagataattg atctccgggt gtgccgcggc ccgggcgagc
    14881 agaaaacgcc ccttgccgca gcccacatcc acctcgaaag gcgcagggcc cgcaaacaga
    14941 tccgccagcc ggaaaggctc cagccagttc tccggcacat atcttctggt ctgctccatg
    15001 ggcgcacctt gacgcataag ccgacagagt aaaccggcgg tttacgatgg acgcaagcgt
    15061 tctttttgcc gtataagggg gcatttaaca gcaggccctg cgacaaaaca cggcagacgg
    15121 gcctttgtgg agaatgaatt ttgtccagaa atcgtcttga aaaactggaa acctatgtgc
    15181 tgggcgtcat tga
//
LOCUS       DAOQWR010000053        14650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4573_length_14650_cov_4.926002, whole genome shotgun sequence.
ACCESSION   DAOQWR010000053 DAOQWR010000000
VERSION     DAOQWR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_14650_cov_4.926002"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKK36_05880"
     CDS             <1..352
                     /locus_tag="PKK36_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNR94124.1"
                     /translation="KKHSSHTLTADVSIAETAAAAEYFLSDGVIVTGKSTGCPADIEE
                     LRAVREAVDIPVLIGSGVTIENVAGYFPLADALIVGSFFKKDGQWANAIDPARVARFM
                     EKVNHIRNIPFDRG"
     gene            complement(361..1806)
                     /locus_tag="PKK36_05885"
     CDS             complement(361..1806)
                     /locus_tag="PKK36_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR94125.1"
                     /translation="MNNWIAGGRIYQINLRSLAAREPRNAIEAAIEQPPVQSPIAYVM
                     QNLSTLKRLGMTILHFMPPFPMGFEGRKGIGSPYAARQYDAVDPEYGTMEEFRQLIAT
                     AHRAGCSVIVGMVPNHTSRDHVWTRSNPDFYVKAADGTPAYDLDWSDTAKLDYRNPGL
                     RAAMIDTYDFWLKLGTDGFRIDMAHFINDRSFWDEAIAALKARHPGKEILFLAECYGL
                     DNSMDLFRRGFNASYDDGMYKILEKHYGVDENLNSVICPGPGADTDLFRLEGVAGAVK
                     RLLGDYRAQTDNLPAPVYWARYTDNHDEGRGVYRFGEQAVRVFSTLFHLLPGSIPFML
                     TGQEFGAANRPSIHERIGPCDKGRRVCTGAGIRSCEGVEFEGNLFARGFDKRREWYEF
                     FRTLMSLRSRCPVLVDGEISLREPAPDDYARRHILIFERRKARDIILCALNFGPEASE
                     VPADLVQGRIIFGALDGSILPAFGFAVSLPG"
     gene            1952..2917
                     /locus_tag="PKK36_05890"
     CDS             1952..2917
                     /locus_tag="PKK36_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNR94126.1"
                     /translation="MKCMVFLLTMILAAGSVRADSAADLVAIRTQLLAHSANTINPDA
                     WGQVMADLDGVILRAGESGDASLTVEASLLKAMALSDMRGEHEQAIRVLRGLIGRYGS
                     QRMPGFQAVYVRLAETHAKTGDSQAILTLIEEFRNSPNYDGQKYDYSGGRSPSDPIRI
                     VRPRGPNGDSITVTSMERALLQAKSAPGRRCPDFRAVDLSGVPVSSRDMDGFLVLIDF
                     WSFRFPRWEQSLPELVRIQKRYENAGFKVIGVPVESNFTESLNALRQAGALWPQLRPD
                     PELMKAFGVFGEIANVLVDPSGRIIGSNLYGIELEAAIRDAIEIQ"
     gene            complement(2921..3733)
                     /locus_tag="PKK36_05895"
     CDS             complement(2921..3733)
                     /locus_tag="PKK36_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR94127.1"
                     /translation="MLGIPIRVHITLWIFLPLIASHISSVLGFTSLFWGLLGAIGLFT
                     SVALHELGHSYVAIRKGCSVREILLVPFGGIAKMQHLPSRPRDEALIAAAGPAVSLML
                     ALVFHLLSRFIGAAGLYALAVTIYVLSAINLMLALFNLLPSFPMDGGRIFRAWMTPKL
                     GRLEATRRAAKIGQTIAIVFGVWAVFGGRFNLSLLAIAIFIYMAAGSEYRAVQLQESF
                     KYRPFGVWGGGVPREPEGNVIVGPAPYEQTSSASSPRNDDDDLFDELWRRWR"
     gene            complement(3877..5079)
                     /gene="thiI"
                     /locus_tag="PKK36_05900"
     CDS             complement(3877..5079)
                     /gene="thiI"
                     /locus_tag="PKK36_05900"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNR94128.1"
                     /translation="MPGAAHKLNPLVLSWDAAMVHFAEIGLKGKNRGDFIRVLAENIR
                     RVLPGEGIRVSEHRDRILVRFANGPAPDDTISAAASVCGVAFVTPVRSVPADLAGMSR
                     AAVQLYRELAWPGATYGVSAKRNCKTFPLTSPEIGRQVGFAVGEATNAPVNLSNPDIP
                     IRFRVYDDKVYLEGPHIAGPGGMPVGVSGRTMCLFSGGIDSPAAAWLMMKRGCRTEFV
                     HFHVFGDPEKVREGKIIPMIQAITRPQGQSARLHLVPYTAFEAGMMQTRVPQELELVL
                     FRRFMARIASRLAVRSRCHAIVTGDNLAQVASQTMQNLTAFDDASSISVFRPLLTYNK
                     QEIIDLSQQLDTFDLSLGKYKDCCSLVARHPHTSPKLETVRRAEGMLPVEDMTVSALN
                     ETVCWTLD"
     gene            complement(5083..6759)
                     /locus_tag="PKK36_05905"
     CDS             complement(5083..6759)
                     /locus_tag="PKK36_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR94129.1"
                     /translation="MRTIKQVLKDSAAERPDATVVRHKVNGQWVEVSYRQLLMRAAQV
                     SQILADAGIRPGDRVALYRENSADWIETYFGIISIGAVAVPIDAKLQGQEVAHILTDS
                     QARVLFSSVEFYRVIERLMGHQHHIEAIVLRDASRMQPPPPPHLGIVDYEELMAGASE
                     KVDAPDSAFGRIDPEEESIASLIYTSGTTGRPKGAMLTHRNFVSNAEAAMQCVNVQRT
                     DNFLLVLPLHHAFAFTANLLLPVAAGAEISLIESLRTMGENMAETKPTVLIAVPLLLE
                     KLDARIQAAIDKSKVGGLLVKMGLGKIIAGKIIAKLGGRIRLIVSGAAPCDPDLLRRW
                     NKMGLTVREGYGLTETAPVLTINTLERNKPGTVGIAMPGIELTIVDPNEQGIGEVAAR
                     GDNVMFGYFNNKEATDEIFRDGALLTGDLGFIDDEGFLTITGRKKNLIVNREGKNIYP
                     EEVESYINKSPYILESVVLGYRDPLDKAGERVGVIAVPDQDAISLHKGKLTDDQVEQL
                     VRSEIKRMVKDISEYKRPRKIQVQFEELEKTSTAKIKRYRYAIDTRQMDA"
     gene            complement(6878..7489)
                     /locus_tag="PKK36_05910"
     CDS             complement(6878..7489)
                     /locus_tag="PKK36_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR94130.1"
                     /translation="MRAKESIITVLDFETTGSVPGWPDQPWQIGMVTLNHGQLDLENT
                     FESLLYIPDRPFNPAAPGNHRLLKKELACAPGLTGLWPELEHRVSEHPLAAHNIGTEK
                     KFLRKLAPLHTLGPWIDTLKLARMVWPELPSHNLGFLLKHLRLKGKTDTLCPGRSEHD
                     AFYDAVGCALLLQHLLDLPGWDRLEIHQLAGAVPREYHRLRSK"
     gene            7578..7904
                     /locus_tag="PKK36_05915"
     CDS             7578..7904
                     /locus_tag="PKK36_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94131.1"
                     /translation="MKMIRMLILLAGTAFCAMAQEPLPEAAVSAETNALLAVKAQSQT
                     TAKRVDRIEVSVAELEKTIGRRAIGSRRPSETIEDQLAEIERRLKRIERDVDDLERRL
                     RRVERR"
     gene            complement(7909..8877)
                     /locus_tag="PKK36_05920"
     CDS             complement(7909..8877)
                     /locus_tag="PKK36_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNR94132.1"
                     /translation="MLLSLSALIAGLALLIWSAGRFVDGSSATAHHLGMSPLLVGMII
                     VGFGTSAPEMTVSAISAWQGNPGIALGNAYGSNICNIALILGLCALVKPVIVHSKVIS
                     RELPLLLLMTVLTAVMLSNGQISRRDAVILLTAFALYMVWCLHESRQLKHDPLSGDIK
                     NELTRHVMPLNKAIFFVIVGLALLIISSRVLVWGAVGIAHALGVSDLIIGLTIVAVGT
                     SLPELASSIAAVQKNEYDIALGNVIGSNMFNTLIVVGIAGIIHPLAVDPMMLKRDVLV
                     MGLLTLAIMVIGCGLRKPGRINRVEGGGLLLCYIGYLTWIVVSAVN"
     gene            9000..9641
                     /locus_tag="PKK36_05925"
     CDS             9000..9641
                     /locus_tag="PKK36_05925"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HNR94133.1"
                     /translation="MTHEYSKDAKVRILNAAIGQFAENGFAATSVRQICDAAGVNVAA
                     VNYYFRSKEQLYHEVFRVLFEDVSAPLTAIPAKVRDAGSWRAALLEWITMALKIVTAD
                     QPPKSSMVKLIAYERTSPSSVFPLLHENFFQPLRDSLAQLIRMGLPKDVDEVTASVWE
                     VSIASQVLVFMHRSPPWDEMLIPRDATREDWLNRTAHHIEEGISSRLSFQGGR"
     gene            9654..11021
                     /locus_tag="PKK36_05930"
     CDS             9654..11021
                     /locus_tag="PKK36_05930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR94134.1"
                     /translation="MKTKTAICGILAALLTSGCARFDPVRARRTQTELFTSSLAEIAA
                     VELAKPLSLEDCIGIAMRSNYEIRKADLNSELRRIGKNVAFTAFLPNVTASAGYKSYE
                     KDPVRMSREYDYAQVDIGMPIFMPSTWFLYAAARHGYASSEMAAAYVRQGIVLETTKN
                     YFNLLVQQETVAALESQREAARANADRIRSLAAEGYFAKWEGEQALFQAEARETELNR
                     ARRQLNVLRGDLLQGMGLSPAADIKLSGETGRIAAPEGTVDELVLKALEIHPELLLAD
                     RAVVVGEHNVRKAFCDFIPTVSFFYSGTWTGDDLVEQAENWVSGFTGVWKLFTGFANT
                     ARYKAAKVERRQSELERESTFLSIMVRVIAAEAALRDAAENARIRQRAYDVAAAKYAD
                     YDARAREGLIPLSEALDALAAMDIAQVELVRSRYQERIAAANLELAMGITLVPQDGNE
                     AKEEK"
     gene            11023..12177
                     /locus_tag="PKK36_05935"
     CDS             11023..12177
                     /locus_tag="PKK36_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR94135.1"
                     /translation="MKVNCVIIGIVSASIMSAGCGRKDAPPEADETVQRLAVRAQAVE
                     TRRFERRLTVQGTLESEKFAEVSARVDGNLDEIWVDEGDAVVAGKTELFQIDPVGRQN
                     ALTIAEQNLEVSRASLEVARATVRKVDAEARKAELDFERYARLYREGKVTDHEHEARQ
                     LAHEQARAGLAVANAQVNLAERHVRQAEASLTIARKNLDDTRSVAPISGRVSARHAEP
                     GELMSVGKVVLRIDDVSSVEAAAFLPSQYHGEVEPGKTRFRLNVSGRDAGMHTISYRS
                     PTINPTLRTFEIKGLVDGQEAAVPGSMADLTIVFEARDGLGVPSSSVLLRAGRPVVFV
                     VTDDRAFSREIQTGLQNDGWTEITSGLEEGDVVVTEGQTQLRDNDAVEVL"
     gene            12190..>14650
                     /locus_tag="PKK36_05940"
     CDS             12190..>14650
                     /locus_tag="PKK36_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR94136.1"
                     /translation="MFLATASTKRPIAMSCLLIALIGLGLNSYRKLSIENMPAVDIPY
                     VAVVTTWVGASPEDVEKDVSKVIEDAVSGIDGLKHIESSSMENVSQVVLEFTLGVNVD
                     IAAQDVREKLDGILSELPAEADRPAIQKININASPIASIFLAGDAPLDDLYDYADNVI
                     ADRFATVPGVGEVQVIGGNEREVWVELDRDRLAAAGLTSADAVAALQGGVLSLPGGRI
                     RERGHEYSVRFDAEYREVADIADLEVASRDGARRKLADLGSVRLASEEIRQRAILDGK
                     PGVVLKIIKKAEGNVVEIARETRKRFEEIQRNLPGGMDLTWVSDESTNIRESVNSALS
                     AVTGAIILCALVLFVFLVNLRTTIVVAITMPVTILISLFFMQLADQTLNMPTLLALGL
                     STGVLVSNSIVVLENIVEKFEAMDDHWEAACIGTSEVAVAVLASAGTNVIVMFPISMM
                     TSLAGRMLAPFAITVLIVNAMSIFISFTLTPILCALMLEPASRRRDNMLTRFSRRWDA
                     GFQRLGGSFTRLLRRIAARKRYNLPVVLGFVFLFLFTMKSGGRSLGFSFFETADEARI
                     FIRIELPPYYELNQTLDRIDRIQARLQEYSDLVHVLTVAGKAESMSGQASEGVYMGQI
                     ELFFKPKTERDWTIQDRLSDIRTRLADETDCLISASVPGRMGGQSFQIEQDLSGDDLD
                     ILEAAALGIQTAVRNIPGVDQIQTTVRDSKPEIRIVPKRTLLSDLGLSSARLAAIVRG
                     NIEGIEAASYKRGDRTYDIRVKLAEAEGKEQIRQFMLPGAEGRTIPLETVADVVDDRA
                     SIQIYRVDKRRTVKILGDIREG"
BASE COUNT         3156 a         4051 c         4354 g         3089 t
ORIGIN      
        1 gaaaaagcac agctctcata cgctgacggc agatgtatcc atcgccgaga cggccgccgc
       61 cgcggaatat tttctgagcg acggcgtcat tgtgacggga aaatcaactg gctgtccggc
      121 ggatattgaa gaactccgcg cggttcggga agcagtggat attcccgtgc tgatcggttc
      181 cggcgtgacc atcgaaaatg tcgccggtta tttcccgctg gcggatgcgc tgattgtggg
      241 atcattcttc aagaaggatg gtcaatgggc caacgccatt gatccggccc gtgttgcccg
      301 gttcatggag aaagtcaacc acatccggaa catccccttc gatcggggat aaacttcagt
      361 tcagcccggc aaactgacgg caaagccgaa tgcgggaagg atgctcccat ccagcgcgcc
      421 gaaaatgatg cggccctgca cgaggtcggc cggcacttcg gatgcctctg ggccgaaatt
      481 caatgcacag agaatgatgt cccgggcttt tctgcgctcg aaaatcagga tatggcggcg
      541 ggcatagtca tcaggcgccg gttcacgcag gctgatctcg ccgtcaacaa gcaccgggca
      601 gcgggagcga agggacatca gggtgcggaa gaactcgtac cattcgcgcc gcttgtcgaa
      661 ccctcgcgca aaaaggttgc cttcgaactc cacgccctcg cagctgcgga tcccggcgcc
      721 tgtgcagacg cggcggccct tgtcgcacgg tccgatgcgc tcatggatgg acggcctgtt
      781 ggcggcgccg aactcctgtc cggtgagcat gaaaggaatg ctgcccggga gcaggtggaa
      841 cagggtgctg aacacccgca cggcctgctc gccaaaacga tagactccgc gcccttcatc
      901 atgattgtcg gtatagcggg cccagtagac gggcgcagga aggttgtctg tttgtgccct
      961 gtaatctcca agaaggcgct ttacggctcc ggcgacaccc tcgagccgaa acaggtctgt
     1021 gtcggcgccg gggccggggc agatcacgga attcaggttt tcatcaaccc cgtaatgctt
     1081 ttcaagaatt ttgtacatcc cgtcgtcgta ggatgcatta aagcctcgcc ggaaaagatc
     1141 catgctgttg tccaatccgt agcattcggc aaggaaaagg atttctttcc cgggatgcct
     1201 ggccttcagc gcggcgatgg cctcatccca gaatgagcgg tcgttgatga aatgcgccat
     1261 gtcgatcctg aacccgtccg taccgagctt cagccagaaa tcataggtgt cgatcatggc
     1321 cgcacgaaga ccgggattcc tgtagtcaag cttggccgtg tcggaccagt ccaggtcata
     1381 ggcgggggtg ccgtcggccg ccttgacata gaaatccgga ttggaccgcg tccacacatg
     1441 atcgcggctc gtgtgattgg gaaccatccc cacgataacg ctgcagcccg cgcggtgcgc
     1501 ggtggcgatg agctgccgga attcctccat ggtgccgtat tcgggatcca cggcgtcata
     1561 ctgccgtgcg gcgtagggtg agccgatgcc tttccggccc tcaaatccca tggggaaggg
     1621 gggcatgaag tgcagaatgg tcattccgag ccgcttgagg gtggaaaggt tctgcatcac
     1681 atacgcaatt ggagactgaa cgggaggctg ttcaatggcg gcttcgatgg cgttgcgcgg
     1741 ttcccgcgca gccaatgacc gcagattgat ctgatatatc ctcccgccgg ctatccagtt
     1801 gttcatcttg cacacctcat catcgcgttg acctgaaaca atagaacgcc atggcggcaa
     1861 ttcaagccgg aagcgaaaag gttcgcattt tttcctgtaa agtgatctta tccagcatca
     1921 attcaatgcg caaagagcgc aggagtcggc tatgaaatgt atggtatttc tcctgaccat
     1981 gattctggcc gccggttccg tccgagcgga ttcggccgct gatctcgtgg ccattcgaac
     2041 ccagctgctg gcgcacagcg ccaatacaat caaccccgac gcatgggggc aggtcatggc
     2101 ggatctcgac ggagtgattc tccgcgcggg agagtcgggc gatgcctcgc tgaccgtcga
     2161 agcttccctg ctgaaggcca tggcgctttc cgacatgcgg ggtgaacatg agcaggccat
     2221 acgcgtgctc cgtggactga tcggccgcta cgggagtcag cggatgccgg gatttcaagc
     2281 tgtttatgtt cgtttggcgg aaacccatgc aaaaaccggg gacagccagg ccattctgac
     2341 cctcatcgag gaatttcgca acagcccgaa ttatgatggg cagaaatatg attactccgg
     2401 aggcagaagc ccgtccgacc ccatacgaat tgttcgcccg cgcgggccaa atggagactc
     2461 catcacggtc acatccatgg agcgtgcact gctccaggcc aaatcggcac cgggccgccg
     2521 ctgtccggat ttccgcgctg tcgatttgag cggcgttccg gtttcttcgc gggacatgga
     2581 cggttttctt gtgctgattg acttctggtc gttccggttc ccccgctggg aacagtcgct
     2641 tccagagctg gtccgcattc agaagcgcta tgagaacgcg ggtttcaagg tcattggagt
     2701 tccggtggaa agcaatttca cagagtcgct caatgcgctc aggcaggcgg gggcactctg
     2761 gccccagctg cgtcccgacc ccgagctgat gaaggctttt ggcgtcttcg gggaaattgc
     2821 aaatgtgctg gttgatcctt cgggacgaat catagggagc aatctctacg gcattgagct
     2881 ggaagcagcc atcagagacg ccatcgaaat ccagtgaacc tcagcgccac cggcgccaaa
     2941 gctcatcaaa cagatcgtcg tcatcgtttc gcggggaaga tgctgaagag gtctgttcgt
     3001 acggggcggg gccaacaatg acattgccct cgggttcccg cggaacgccg ccgccccaga
     3061 ctccaaacgg tctgtatttg aatgattcct gaagctgcac tgcgcggtat tccgaacccg
     3121 ccgccatgta gatgaaaatc gcaatggcca gcagggacag attgaaccgc cccccgaaga
     3181 ccgcccacac gccgaagacg atggcgatgg tctggccgat ctttgccgcc cggcgcgtgg
     3241 cttccagccg gcccagtttc ggggtcatcc atgcgcggaa gattcgcccg ccatccatcg
     3301 gaaaagaagg aagaaggttg aacagcgcca gcatcaggtt gatggcgctc aggacatata
     3361 tcgtgacagc cagcgcatac agaccggcgg ccccgatgaa gcggctgagc aggtgaaaca
     3421 caagcgcaag catcagactg acggccggtc cggccgcagc gatgagggct tcgtcgcgcg
     3481 gtcgagacgg aagatgctgc attttggcga tgcccccgaa agggaccagc agaatttcgc
     3541 gaacgctgca gccttttcgg atggccacat acgagtggcc cagctcgtgc agagccacgc
     3601 tggtgaacag cccgatcgcg ccgagcaggc cccagaacag ggatgtaaaa ccaagcaccg
     3661 atgagatgtg cgaggcaatc aggggcagaa atatccacag ggtgatgtgc acccttattg
     3721 gtatgccaag cactgtccct atttttgttg aactggccat gtcaggggag aatgtagcgc
     3781 agattgccgc cattgcaagg atggatgact tcatctcagc ggcgtcgtgc tccaggcatc
     3841 atcccggggg cggcgagtac cacaaacctt ctgaaatcag tccagcgtcc agcagacggt
     3901 ctcgttcagg gcggagacgg tcatgtcctc gaccgggagc atgccttcag cccgtcgcac
     3961 ggtttccagt ttcggagagg tgtgcggatg cctggcaacg agcgaacagc agtccttgta
     4021 tttcccaagg gataaatcaa acgtgtcaag ctgctggctg agatctatga tttcctgttt
     4081 gttgtatgtc agcagagggc gaaagacgct gatgcttgag gcgtcatcga atgctgtcag
     4141 gttttgcatg gtctgcgaag cgacctgcgc caggttgtct ccggttacaa tggcatgaca
     4201 cctggaacgg acagcaagcc gtgaagcgat gcgggccatg aagcggcgga acagcaccag
     4261 ctccagctcc tggggaaccc gtgtctgcat catgccggct tcaaaagccg tgtaggggac
     4321 cagatgaaga cgggccgact ggccctgcgg gcgggtgatc gcctgaatca tgggtatgat
     4381 tttgccttcg cggacctttt ccgggtcgcc gaatacatgg aaatgaacaa attccgttct
     4441 gcatccgcgc ttcatcatca gccaggcggc ggcgggagag tcaatgccgc ccgagaacag
     4501 gcacatggtc cgtcccgaaa cgccgacggg catgcctccc ggccctgcga tatgcggtcc
     4561 ctccagatag accttgtcat catagacccg gaagcggata gggatgtccg gattggacag
     4621 attgaccggt gcgtttgtgg cttcgccgac ggcaaagccg acctgccggc ctatttccgg
     4681 agaggtaagg ggaaatgtct tacagttgcg ttttgcggat acaccgtagg tggcccccgg
     4741 ccatgcgagt tcacggtaca gttgaaccgc ggcacggctc atgcccgcaa ggtccgccgg
     4801 cacgctgcgc acaggggtca caaaggccac gccgcagacc gatgccgcgg ctgagatggt
     4861 gtcatcagga gccgggccat ttgcgaaacg aaccagaatc ctgtctcggt gttcgcttac
     4921 tctgatgccc tctccgggca gcacccgccg gatgttttcc gcgagtacgc ggatgaagtc
     4981 gccccgattt ttccccttca gaccgatttc agcgaaatgc accatggcag catcccacga
     5041 cagcaccaga gggttgagtt tatgagcggc tcccggcatt tctcaggcgt ccatttgccg
     5101 ggtgtcgatc gcatagcgat agcgcttgat cttcgcggtg gatgtttttt ccagctcctc
     5161 aaactgcacc tgaattttgc gcggacgctt atattcggaa atatctttca ccatgcgctt
     5221 gatctcactg cggaccagct gttccacctg atcatccgta agttttccct tgtgcagact
     5281 aatggcgtcc tgatccggaa ccgcaataac acccacgcgc tctccggcct tgtccagcgg
     5341 atcacgataa cccagcacaa cggattcgag gatatacggg ctcttgttga tgtacgattc
     5401 gacctcttca ggatagatat tcttgccctc gcggttcaca atcaggtttt tcttccgtcc
     5461 agtgatggtc aggaagccct cgtcatctat gaacccgaga tcgccggtca gcagggcgcc
     5521 gtcgcggaat atttcatcgg tggcttcctt gttgttgaag tatccgaaca tcacattgtc
     5581 accgcgcgcg gcgacctccc cgatgccctg ctcattgggg tccacaatgg tcagctcaat
     5641 gccgggcatc gcaatgccca ctgtgccggg cttgtttctt tccagtgtgt tgatggtgag
     5701 caccggcgct gtttccgtga gcccgtagcc ctcgcgcacc gtgagcccca tcttgttcca
     5761 tcggcgaagc aggtcgggat cgcagggagc ggctccgctg acaatcaggc ggatccggcc
     5821 gcccagtttg gcgatgatct tgccggcaat gattttacca agccccatct tgaccaaaag
     5881 tccgcctact ttgcttttat caatggcggc ctgaatgcgc gcatccagct tttcaagaag
     5941 aagaggcaca gcaatcagca cggtgggttt tgtttcagcc atgttctcgc ccatggtcct
     6001 caggctttcg atgaggctga tttccgctcc ggcggcaacc ggcagcagga gattggcggt
     6061 aaaggcaaag gcgtgatgca gggggaggac cagcagaaaa ttgtccgtgc gctgcacatt
     6121 cacacactgc atggcggcct cggcattaga cacaaaattc ctgtgcgtca gcatggcgcc
     6181 cttggggcga ccggtcgttc cagaggtgta gatgagcgag gcgatggatt cctcctccgg
     6241 gtcaattctt ccgaaagcgc tgtcgggggc atccactttt tcggaagcac ccgccatcag
     6301 ctcctcgtaa tccacaatgc cgagatgggg cgggggtggc ggctgcattc tggaggcgtc
     6361 gcggaggaca atggcttcga tatgatgctg gtggcccatc agccgctcga tcacgcgata
     6421 gaattcaacc gaagagaaca gaacgcgagc ctgggaatcc gtgaggatgt gggccacttc
     6481 ctgcccctgg agcttggcat ctatgggaac cgccaccgcg ccaatggaga tgatgccgaa
     6541 gtaggtttcg atccagtctg ccgaattttc ccggtacaga gccacgcggt ctccgggccg
     6601 gatgcccgca tccgcgagaa tctgcgacac ctgcgccgcg cgcatcagca gctgccgata
     6661 ggagacttca acccactggc cgttcacctt gtgccgaacc acggtggcat caggccgttc
     6721 cgcagcagag tccttcagta cctgtttgat tgtgcgcatg tttggttctc ccagctgtca
     6781 atccgcatcg taaggcaggg ccttcttcaa agtcgagaca aaagccggga cccgggatgc
     6841 agctgcagac tgcttcagat ttcgggacgg gagcggatca tttactccgc aggcggtgat
     6901 actcgcgcgg gacggctcct gcaagctggt ggatttccag tctgtcccag ccgggcaggt
     6961 ccagcaggtg ctgaagaagc agggcgcatc ccacagcgtc atagaatgcg tcgtgttcgg
     7021 accggccggg acacagggtg tcggtttttc ccttcaggcg cagatgcttc agcagaaaac
     7081 ccagattgtg cgaaggaagt tccggccaga ccatgcgcgc cagcttcaga gtgtctatcc
     7141 atggtcccag tgtgtgaagc ggggctagtt ttcgaaggaa tttcttctcg gtgccgatgt
     7201 tgtgcgcggc aagagggtgc tcggagacgc gatgttccag ttccggccac agaccggtca
     7261 gccccggcgc gcatgccagt tccttcttca ggaggcgatg gtttccgggc gctgcgggat
     7321 tgaacggacg gtcgggtatg taaaggaggg actcaaaggt gttttcaaga tcaagttgac
     7381 catggttcaa tgtgaccatc ccgatttgcc atggctgatc gggccagccg ggcactgaac
     7441 cagtggtctc gaaatccaga acggtgatga tgctttcctt tgcccgcata acagaccgga
     7501 ctctatcgtc atgagcccgg gatggacagg aaaatatcgg catgctataa tatctataat
     7561 aaaggagaaa gcaggccatg aagatgataa gaatgctgat cttgctggcg gggacggctt
     7621 tttgtgcaat ggcgcaggag ccgctgccgg aggccgctgt gagcgcagaa acaaacgccc
     7681 ttctggcggt gaaggctcaa agccagacga ccgcaaaacg ggtcgatcgt atcgaagtat
     7741 ctgtcgcaga gcttgaaaaa actatcggga gacgggcgat cggaagccgc cgtccgtcgg
     7801 aaaccatcga agatcagctt gctgaaatag agcgcagact caagcggatc gaaagagatg
     7861 ttgatgatct ggagcgcagg ctccggagag tggaacgccg gtaggccttc aattcacggc
     7921 gctgacgaca atccatgtta gatagccgat gtagcacagg agcagacctc cgccttccac
     7981 cctgtttatc cgtccgggtt tgcgcaaacc gcatccaatg accatgatgg ccagtgtcag
     8041 gagccccatc acgagaacat cccgtttcag catcatggga tccactgcca agggatgaat
     8101 aattcccgca ataccaacca caatcagggt attaaacatg ttcgatccga tcacattacc
     8161 aagtgcgatg tcgtactcat ttttctgcac tgcggcaatg gaagaggcca gttccggaag
     8221 agaagtgccc acagcgacaa tggtgagtcc aatgatgagg tcgctcacgc ccagtgcatg
     8281 agcgatgcct accgctcccc agacgagcac ccgtgaactg atgatcagca gagctagccc
     8341 gacgatgacg aagaaaatgg ctttgttcag aggcataaca tgacgggtca gctcgttctt
     8401 tatatctcct gaaagcggat catgcttcaa ctggcgactt tcatgcaggc accagaccat
     8461 atagagcgca aatgccgtca gaagaatgac ggcatccctt cgggaaattt gtccatttga
     8521 aagcatgacg gcggtcagca cggtcatgag cagcagcaga ggcagttcac ggcttatgac
     8581 ctttgagtga acaatgacgg gcttaaccag ggcacagagg cccagaatca gcgctatgtt
     8641 gcagatattg gacccatagg cattgcccag agcaattccg ggattgccct gccatgcgga
     8701 gatggcagaa acagtcattt ccggggccga tgtcccaaag ccgacaataa tcatcccgac
     8761 aagcaggggt gacatgccca aatgatgcgc tgtggcggaa gagccgtcca caaaacgtcc
     8821 ggcgctccag ataaggagtg ccagcccggc aatcagagct gaaagagaca aaagcataat
     8881 atcgcggata ttaagggcgc gcaggattgt aaacaagaaa ttatgcagaa cagttgacaa
     8941 gaccagatgc accgagtaca agtaaaacaa ttgtttaata caactgtttg tgcagggata
     9001 tgacacacga atacagcaaa gatgcgaagg taagaattct gaacgcggcc atcggccagt
     9061 ttgcggagaa tggcttcgcg gcaaccagcg ttcgccagat atgtgacgcg gcaggggtca
     9121 atgtggccgc agtcaattat tatttccgtt cgaaggagca gctttatcac gaggtcttca
     9181 gggttctctt tgaggatgtc agcgcccctt tgacggccat tccggcgaag gtgcgtgatg
     9241 cggggtcatg gagggcggca cttctggaat ggatcacgat ggccctgaaa atagtcaccg
     9301 ccgatcagcc gccaaaaagc tcgatggtga aactgattgc ctatgagcgc accagtccgt
     9361 caagcgtgtt cccgctgctt catgaaaatt tttttcagcc tttgcgcgat agtctggcgc
     9421 agctgatccg aatgggtctt ccgaaggatg tggatgaggt gacagcctct gtctgggagg
     9481 tgtccattgc ctcccaggtt ctggtgttca tgcaccgcag ccccccgtgg gatgagatgc
     9541 tgatcccacg ggacgcgact cgtgaagact ggcttaaccg cacggcgcac cacatagaag
     9601 aggggatatc ctcccggctt tcttttcagg gcggccggta accagaggcg tttatgaaaa
     9661 ccaaaacagc aatctgcgga atccttgcag ccctgttgac ctccggctgc gcgcggtttg
     9721 atccggtccg cgcgcgccgc acccagaccg aattgttcac cagctccctg gcggagattg
     9781 cggctgttga acttgcgaag cccctttcgc tcgaggactg catcggcatc gccatgcgca
     9841 gcaactatga gattcgcaaa gcagacctca acagcgaact gcgccgcatt gggaagaatg
     9901 tggctttcac ggcttttttg ccgaatgtca ccgcatcggc cggttataaa tcgtatgaaa
     9961 aagatccggt gcgcatgagc cgggagtatg attatgccca ggtggatatc gggatgccga
    10021 ttttcatgcc gtccacgtgg ttcctttatg cggcggcccg tcatggctat gcgtcctcgg
    10081 aaatggctgc ggcctatgtg cgtcagggca ttgtcctgga aacaacgaag aactatttta
    10141 atcttctggt tcagcaggaa accgtggccg cccttgaatc gcagagggaa gcggctcgcg
    10201 caaatgccga ccgtatccgc agtctggccg cagagggtta cttcgcaaaa tgggaggggg
    10261 agcaggccct ttttcaggcc gaggcgcgcg agacggaact gaatcgcgcc cgcaggcagc
    10321 tgaatgttct ccgtggtgat ctcctccagg gcatgggcct ttcgcccgct gccgacatca
    10381 agctctccgg ggaaacagga cgtattgccg cgccggaagg cactgtggat gagctggtgc
    10441 tgaaggctct ggagattcat ccggaactgc tgctggccga ccgggcggtg gttgttggcg
    10501 agcataacgt gcgcaaggct ttctgcgatt tcatcccgac ggtttctttc ttctactctg
    10561 gaacatggac gggcgatgat ctcgtcgagc aggcggagaa ctgggtgtcc gggttcaccg
    10621 gagtgtggaa gctgttcacg ggctttgcga acacggcccg ctacaaggcg gccaaagttg
    10681 aacgccggca gagcgagctg gagagggagt caacgtttct ctctatcatg gtgcgggtca
    10741 ttgccgcgga agcggctctt cgggatgccg ctgaaaacgc ccgcattcgc cagcgggctt
    10801 atgatgttgc agccgcgaaa tatgcagatt atgacgcgcg ggcccgtgaa ggacttattc
    10861 cgctcagcga ggcgcttgac gcgctggccg cgatggatat tgcgcaggtg gagcttgtgc
    10921 ggagccgcta tcaggaacga attgcagctg ccaatcttga gctggccatg ggcattaccc
    10981 ttgtccctca ggacgggaat gaagcaaagg aagaaaaata gcatgaaagt gaattgcgtc
    11041 atcatcggga ttgtcagtgc ctccattatg tccgcgggct gcggcagaaa agatgcccct
    11101 cccgaggcag atgaaactgt gcagagactt gcagtgcgcg cgcaggccgt ggagacccgc
    11161 cgcttcgagc gccgcctcac cgtgcaggga acgctcgaga gtgaaaaatt tgctgaagtt
    11221 tcagcccgtg tggacggcaa tcttgatgaa atatgggtgg atgagggcga tgccgttgtt
    11281 gccggaaaga ctgagctttt tcagatcgat cccgttggcc ggcagaatgc cctgaccata
    11341 gcggagcaga atctggaagt cagcagggct tcactggaag tggcccgggc cactgtgcga
    11401 aaggtagatg ccgaagcgcg caaggctgag cttgattttg agcgctacgc gcgcctgtac
    11461 agggaaggca aagtcacaga tcacgaacat gaggcgcgtc aattggcgca cgagcaggcc
    11521 cgggccgggc ttgccgttgc gaacgcgcag gtcaatcttg ccgagcggca tgtcaggcag
    11581 gccgaggctt cactgaccat tgccaggaag aatcttgatg ataccagaag cgttgcgccg
    11641 atttccggca gggtgagtgc ccgtcacgct gaaccaggcg agctgatgtc ggtcggaaag
    11701 gttgtgctcc gaattgacga tgtgagttcc gtggaggcag ccgcctttct tccctcgcag
    11761 tatcacggag aagtggagcc tgggaaaacc cggttccgcc tgaatgtgag cgggcgcgat
    11821 gccggcatgc acacgatcag ctatcgcagt cccacgatca acccgacgct gcgcaccttt
    11881 gaaatcaagg gccttgttga tggtcaggag gcggccgttc cgggaagcat ggccgacctt
    11941 acgattgttt tcgaggcgcg tgatggtctc ggtgtgcctt cctcgtccgt gctgctccgg
    12001 gccggaaggc cggttgtttt tgttgtaaca gacgaccggg ccttctctcg tgaaattcag
    12061 accggacttc agaatgatgg atggaccgag attacttccg ggcttgaaga aggggacgtg
    12121 gtcgtcaccg aggggcagac acagctccgg gataatgatg ccgtagaggt gctgtaagga
    12181 gatcaagcca tgtttctagc caccgcctcc accaaacgcc cgatcgcgat gagctgcctg
    12241 ctgatcgccc tgattggtct tgggctcaat tcctatcgca agttgtccat cgagaacatg
    12301 ccggccgtgg atatccccta tgttgccgtt gtcaccactt gggtcggagc ctcccccgag
    12361 gatgttgaga aggatgtcag caaggtgatt gaagatgccg tctcgggcat tgacggcctc
    12421 aagcatattg agtcctccag catggaaaac gtttcacagg ttgttctgga gttcacactg
    12481 ggcgtaaacg tggacattgc cgcccaggac gtgcgcgaaa aactggacgg gattctctcc
    12541 gaactccctg ccgaagccga ccgtccggcc atccagaaga tcaacatcaa cgcttcgccc
    12601 atcgccagca tatttcttgc gggagacgct ccgctggatg atctttacga ctacgcagac
    12661 aatgtcattg ccgaccgttt cgccaccgtg cctggcgtgg gcgaggtgca ggtcatcggc
    12721 ggcaacgagc gtgaagtctg ggttgaactg gaccgggacc ggcttgccgc cgccggactc
    12781 acttcggccg atgcggttgc cgctttgcag gggggtgttc tctcccttcc cggtgggcgc
    12841 atccgtgaaa ggggtcatga atactctgtc cgtttcgacg ccgaataccg ggaggtcgcg
    12901 gacattgcgg accttgaagt cgccagtcgc gacggtgccc gccggaaact tgctgacctc
    12961 ggttctgtcc gtctcgcctc ggaagagatc cgtcaacgag ccattcttga cgggaagccc
    13021 ggtgtggttc tcaagatcat caagaaagcc gaaggcaatg ttgtcgaaat cgcaagggaa
    13081 acccggaagc gatttgagga aattcaaagg aatctgcccg gaggcatgga ccttacctgg
    13141 gtctccgacg aatccaccaa catccgcgag tcggttaatt cggcgctctc cgccgtgacg
    13201 ggagccatta tcctctgcgc tctggtgctt tttgtttttc tcgtcaattt gcggacaacc
    13261 attgtggtgg ccatcacaat gccggtcacc atattgatca gtcttttttt catgcagctg
    13321 gccgatcaga ccctgaacat gccaaccctg cttgcacttg gcctttccac gggcgtgctg
    13381 gtttccaact ccatcgtcgt actggaaaac attgtcgaaa agttcgaagc aatggatgat
    13441 cactgggagg ccgcgtgcat cggcacctcc gaagttgctg ttgccgttct ggcctcggcc
    13501 gggacaaacg tcatcgtgat gtttcccatc tcgatgatga ccagtctggc gggtcgtatg
    13561 cttgcacctt ttgccatcac ggttctcatt gtcaatgcca tgtccatctt tatcagcttc
    13621 acgcttacgc ctattctctg cgccctgatg ctggaaccgg catcaaggcg acgggacaac
    13681 atgttaacgc ggttcagtcg gaggtgggat gcgggttttc agcgtctggg cggcagcttt
    13741 acgcgccttc tccgccggat tgccgcgcgc aagcgctata atcttcctgt tgttcttgga
    13801 tttgttttct tgttcttgtt caccatgaag tcaggcgggc gcagtctggg cttcagcttc
    13861 tttgaaaccg cagatgaagc ccgcatattc atccgaatcg aacttccgcc ctactatgag
    13921 ttgaaccaga cgcttgaccg cattgaccgc attcaggccc ggctgcagga gtacagtgat
    13981 cttgttcacg ttctcaccgt ggccggaaag gctgaatcca tgagcgggca ggccagtgag
    14041 ggcgtgtaca tggggcagat tgaattattc ttcaagccca agactgagcg cgactggacc
    14101 attcaggacc ggctttcaga tattcgcacc cgcctggccg atgaaaccga ttgtctgata
    14161 tctgcttctg taccgggacg catggggggc cagagtttcc agattgaaca ggacctgtca
    14221 ggcgacgatc ttgacattct ggaggctgcg gcgcttggta tacagaccgc tgttcgcaac
    14281 atccccggcg tggatcaaat tcagaccacg gtgagggatt ccaaacccga aatccggatt
    14341 gtgcccaaac gaacgctgct ttccgatctt ggcctgtcct ccgcgcgcct cgcggccatt
    14401 gttcgcggca atattgaggg aattgaggcg gccagctaca aacgcggaga tcgcacctat
    14461 gacatacggg tcaagcttgc ggaggccgaa ggcaaagagc agatccggca gtttatgctg
    14521 cctggcgccg agggccgcac cattccgctc gaaaccgttg ccgacgtggt tgacgatcga
    14581 gccagtatcc agatctaccg ggtggacaag cggcgcaccg tcaagatact tggagacatc
    14641 cgtgaaggcg
//
LOCUS       DAOQWR010000054        14543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4613_length_14543_cov_5.862714, whole genome shotgun sequence.
ACCESSION   DAOQWR010000054 DAOQWR010000000
VERSION     DAOQWR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14543
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4613_length_14543_cov_5.862714"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2456
                     /locus_tag="PKK36_05945"
     CDS             <1..2456
                     /locus_tag="PKK36_05945"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNR94137.1"
                     /translation="ERIITAPQQVTSVFGPVDLSQEPAPLYIGERANATGSKKFRDAL
                     LKDDYDACFAILQEQEETGAHVLDLSMAYTGRDEVKDVEHLVSRAAKECRLPLMIDST
                     QTDVIEAALKLYGGRMIVNSINFESGEERAETVTALARAYGAALVALTIDETGMARTA
                     DEKFRIAERLVKFCEARGVSRTALFIDPLTFTVGSGDETLRTAAVETVAAIRRIKKDL
                     PGVRTVLGLSNISFGLKPAGRKVLNAVFLDRCVKAGMDACIINVAGIVPLTEISADAL
                     KVAGALLDNDQSAGDPLENFINFFSDEQAEDASAAAAAKEPEDILTEAVVRGRLQPLG
                     DVMPGLLQKYSAEHILNEILVPAMQEVGRLFNDGILQLPFVLKSAEVMKKSVDLIKPH
                     MKTSDTGARRATMVLATVAGDVHDIGKNLVDIILSNNGFHVVNLGTKMPVEQMIAAVK
                     EHRVDVLGMSGLLVKSAAQMAENMKALDAAGLAIPVFLGGAALTAEYVANTCQPAYSG
                     PVIYCKDAFEGLTRMQELVSKGRLEKTPPIKRPAAGPAELRTAPSEPVEQIEPPTPPF
                     WGHRINRDINLEDVYPMLNEKSIVRGQWKFRKGKLTDREWQKLMDEEVNPRMEELKKK
                     FRNRDLFQPQTAWGFYPCRSEGNSLFVFADEKSEPIRLDFPRQRKPPHKAIPDYFRAD
                     RDVVGFMSVTLGRGPDRETQRLLKEDRYQDYYLLYGFAVELTDALAEYCHKTMRLAWG
                     FDEPEMDLQGYNDQLYRGSRYGFGYASCPDLAMNRIACDLVHAKEMGVEVSENLMMIP
                     EFTTAAIVLHHPQAKYFNV"
     gene            2469..3014
                     /locus_tag="PKK36_05950"
     CDS             2469..3014
                     /locus_tag="PKK36_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNR94138.1"
                     /translation="MRHLKKLSEIDWKTWKPKERATLLFVIRDGQALLIDKKKGLGAG
                     KVNAPGGRVEKGETAQEAAIREVEEELCVTPLNVSPRGRLNFQFTDGYSLQGHVFWAT
                     DCRGEPQETCEAVPRWTPLDEIPYDKMWADDLHWIPLMLEGKHFEGHFIFDDDTMLDH
                     TLITFTPEEWAIECRNGSGEM"
     gene            2987..3829
                     /locus_tag="PKK36_05955"
     CDS             2987..3829
                     /locus_tag="PKK36_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94139.1"
                     /translation="MQEWIWRDVRLEIPGDWEMLQFSRARDKGRCAYADRRQFRLELS
                     WRQVKGAPDFPRMMADYKARLESAGELQDAHTFTLSGWHGLAGMQKNLRVSRFGNYFD
                     KENLLAELVFLWPDKRDIPLENDILQSCRYAPPENGFRKIKAFGMSCRSPEKYEFTAC
                     SVQPAMAAMVFSGARRPARIRLQRLGMVPQWLDQPLTTWAGTQLPKEARNPDFESRSR
                     NGHEIVTAKADYAPRGFLIRKGLYAASAWICPKDGRLYHAEQICRSAKDIVNHPLDCC
                     GGMS"
     gene            3826..4215
                     /locus_tag="PKK36_05960"
     CDS             3826..4215
                     /locus_tag="PKK36_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNR94140.1"
                     /translation="MMAGFSNPSWSDMLDALMIRNRAARVERDARSGEIRIAVPTRRP
                     WYHVPPVTWIVRAPEERVTVLDEPGSLVWEMCDGRTTVENIIDAFAEKYGLTFHESRV
                     AVTQHIKSLISRGVLAVEFRDPPAPGL"
     gene            complement(4295..7489)
                     /locus_tag="PKK36_05965"
     CDS             complement(4295..7489)
                     /locus_tag="PKK36_05965"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNR94141.1"
                     /translation="MRFPVTQKMLVDWAGARVFREGKTLCETGRVEHVSYEHPFVRGS
                     LIYGNRNMTCQFELLKDGSVASQCPCRDNTERGIICAHVIALALIMIKRQTDPERERK
                     YEEERRRAERLSSTDESQYLRRVPSGTAGARPCRPMLILDPQWPSAGAEGFPLSICYK
                     TSGGLVPADQMDLSVPVAVSRRDDNLLFVLEDIAEGPVPARLSVQASDFINLIKLLAG
                     RPLYEGSADHPLTVNAVKMNSVIRMHLDSASGELVLMIHTELPFMDAACLPTYIVSGK
                     EGWVFDAGQFWPLGNVLPAPLQNIYRNPVRIARPSVPRFMQTELPLIEAHIRVETDVT
                     FDLFTIEPAEPRFTLNVRGSPASLSAQLVAVYGEHRLPAGKADAAGQFALPDPEDLMR
                     YLVRNPAREAEALEILRAIGFGGEHGDSLAAVIGCREVLNFLGSGLPKLRRMGWTVAL
                     AGRVEPFMDELDFTTPVISIESGEEGRWFEVGFRYEDTRGQSLSEAEIQRALLKGDAY
                     VEKNGRTILLDADAIQTARDVFTDCAAGEGSRPGLFRLEGLYSAYVQASLDSLDGVDV
                     EAAPAWREKAREQNRGVPLQPVPLPGNLDGVLRHYQKDGVNWLRFLESNYLCGILADE
                     MGLGKTLQTLAWLRLPRVHDEAAGRPALIVCPTSLVENWAEEAGRFVPDMRVLTLSGA
                     ERHEKRDKLEHADIVITSYALLRRDIEQYLNCSFSCAVLDEAQHIKNRSTQNALAAKK
                     LNAVHRLVLTGTPIENSVADLWSIMDFLMPGYLGRHPSFREHYELPIAGGGPEADMTQ
                     TGLRRKLQPFLLRRLKKDVAKELPPKIERIAPCLLTADQQLVYKKILEQSRRKISGMV
                     SARGFNSSRMEIFKTLLRLRQICCHLELLKLKDLDSRYPSAKLDLFFELLDEALDAGH
                     RVLVFSQFTSMLAILKRELAARERTYCYLDGATKDRMGVVREFNTNRDIPVFLISLKA
                     GGTGLNLTGADMVIHYDPWWNPAVEDQATDRAYRIGQKRTVYSVKLITHGTVEEKVLE
                     MQKRKKAVINATLAPAGQMMESLTWEDVRELLDI"
     gene            7636..8238
                     /locus_tag="PKK36_05970"
     CDS             7636..8238
                     /locus_tag="PKK36_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR94142.1"
                     /translation="MTMETKRAQAIKHLEGRKSLPIIEVFYEFNHLKSLYRQGWLRVG
                     IPREHCETVAEHTLGVTLLALFLADEYFPELDKWKLMLMGLLHDFGEIYAGDIVPGKM
                     TLEDKHRLERKSVEQVFARLPKGNDYLAVWEEFEAGQTAEARFMKEIDRLEMGLQASV
                     YEHEKLGDLSVFFDSTDRALKTGRLRDILDALRRLRATAP"
     gene            complement(8246..8986)
                     /locus_tag="PKK36_05975"
     CDS             complement(8246..8986)
                     /locus_tag="PKK36_05975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR94143.1"
                     /translation="METQEYKIKDVMKDEKQSAFKKYRSVYYGDMSVGRVILCEVIIT
                     LFGWIPGALGLALRKLTYPFMFEDIGRKVVFGRNITIRHPHKIEIGDNVVLDDHCVVD
                     AKGETNEGIEIGDNVYVGRNTIIYCKNGSITIGDNVNLSSNCQVFSSNKLTLGRDTVI
                     GAFCYFLSGGEYDYTDREHTFAQQSGTNTKGELTIGPNCWFGARVTVLDAAGIGEHCV
                     LAAGAVVTKPIPPNSLAAGVPARVIKEI"
     gene            complement(9000..10916)
                     /gene="asnB"
                     /locus_tag="PKK36_05980"
     CDS             complement(9000..10916)
                     /gene="asnB"
                     /locus_tag="PKK36_05980"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNR94144.1"
                     /translation="MCGIAGVMAPGHRNIISKLTDTMKHRGPDDEGFYHDDTISIGQR
                     RLSIVDVAGGHQPICNEEGNLQLVCNGEIYNSPELRRDLEARGHVFKTHTDVETALHL
                     YEEYGANCTKHLRGMFGIAIWNARDKSLFLARDHMGQKPLFYYFDGNIFLFASEVKAI
                     LASGLVKPELDLTAIWHYMSLRFIPDDRTLFKNIKKLPAATSLFFDGREVKTEQYWTL
                     DFKNKISGSEKDVTDELEHLLRETVKLHLLSDVPVGTFLSGGIDSSLVTALMAEITGE
                     AFPTFSIGVKEQGFNELPYARMVSDKYRLTARERIVEADMINLIPSMIHHMDEPSDPF
                     GVGVYLVSQVAAEEVKVVLSGDGGDENFAGYDRFAGQRMVDFYCLLPAWFRRTVMKKL
                     TSMVPESFGYKSLAQKVAWVNDMSFYEHGERYAQSASFLRFTPEHKDSLFTGPAKTGI
                     ADADSFQKILTHFNCGRAEELVDQMLYTDLMTRMPDHLLPIVDRMAMAHSLEARPPLI
                     DHKVTEFAASIPGGMKLKGRDMKHILKQVASRHLPQELIYRKKQGFGFPIALWMRSEL
                     STFLKNLFAQSKFVELGIFDGAYVDRLLDEHLSGKRDHNFRLWILLNLEIWYRLYFEG
                     ETVESMKAFIASIR"
     gene            complement(11076..11843)
                     /locus_tag="PKK36_05985"
     CDS             complement(11076..11843)
                     /locus_tag="PKK36_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNR94145.1"
                     /translation="MKSRRFIVVTLMMLGLSACAQTERDPYHYAGAPVAASNRFEKIT
                     LLKNRGYAVGYDEARRNPAWVAYRLHRVRNRPDTSRRDRYNTDMRTSAKVSNNDYSNS
                     GYSRGHMAPSFAMAVCYGKKAQNQAYLMSNMTPQKVPFNGGPWQELEHQELNNYAFNF
                     GEIWVVTGPVYDQKIEKIPGGPEIPDAFFKIILDEKDNAIRAIAYLMPHEALSKELKY
                     YLTSIDRIEQLTGLDFLPNLPDHVENRLEAMIAAEPW"
     gene            complement(11840..12574)
                     /locus_tag="PKK36_05990"
     CDS             complement(11840..12574)
                     /locus_tag="PKK36_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94146.1"
                     /translation="MQEADAVFPETADIETSSDDYAGRFAGAAGQWLLEVQERITMRF
                     LRGHPGARVLDVGGGHGQLAIPMCKAGYDVTVLSSDESCKKRIREIVDSGQCRFLVGN
                     VIALPFPDNSFDVVIAFRMVTHCEQWPVLIRELCRVAKSAVIVDYPTSQSLNAVAPAL
                     FNAKKKIETNTRTWILFRHRQIMDAFRDSGWHLHARRGQFFLPMVLHRMLKCRGLSAF
                     LEGACRALGLTRLWGSPVIIEMRPPA"
     gene            12656..13420
                     /locus_tag="PKK36_05995"
     CDS             12656..13420
                     /locus_tag="PKK36_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94147.1"
                     /translation="MPILADTHIHVYPFYELSLFFGGAFRKFAALEEGADHVLFLAER
                     HDCHFFRQLKEGLSKVSGMECAAGEEVLTIRNRNGKRLWLAAGRQIVTAERLEILALT
                     TDAEIPDGQPARDVIRRIREIGAVPVIAWAPGKWFFGRGKVVRALIDSEPAGSFLLGD
                     STLRPTVWPEPLLMRRAKKKGFKVVAGSDPLPVPGEEAKAVSYGIRCDGNIDPAQPLK
                     SLRDMLTNLPGAKNFGRRDSLLSVLTRLKKNSSAKS"
     gene            13417..>14543
                     /locus_tag="PKK36_06000"
     CDS             13417..>14543
                     /locus_tag="PKK36_06000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94148.1"
                     /translation="MNDKMRRVLFISPQPYFQWRGSPIRVAFDAQALSEAGWMVDLVV
                     MPVGEDHPLPNVAVHRPMNIFRINKLAIGPSAVKAFLDIFLFCTALKLAFKNRYDIIH
                     GVEEAGAMAVVLGKLRGAKVIFEKHSDPASYKKGFVRNAVMWLYSQVEKFSIRHADAV
                     IGTGPALVEQARKINPRITAHHIFDIPSSLVEAVPEKIAAACAKLERKPGDRIITYVG
                     SFAVYQGIGLMFESIPPVVQARPEARFVIIGGTEQEIAERRAWLAERKSEHAVMFPGK
                     IPPDELPNYLSASDILLSPRTSGTNTPLKLLDYLKAGRAIVACDIPANRLIVDEETAL
                     LVPADPDSFSKGIIKLLDNDPLRARLAGSGRRLVDETYNFRV"
BASE COUNT         2927 a         4366 c         4375 g         2875 t
ORIGIN      
        1 cggaaagaat aatcaccgcg ccccagcagg tcaccagcgt gttcgggccc gtggatctca
       61 gccaggagcc ggccccgctc tatatcggtg aacgggccaa cgccacaggc tcaaagaaat
      121 tccgcgatgc gcttttgaaa gatgattacg acgcctgctt cgccatcctg caggagcagg
      181 aggaaaccgg agcgcatgtg ctcgacctca gcatggccta caccggccgc gacgaggtga
      241 aagatgtgga acatctcgtc agccgcgccg ccaaggaatg ccgcctgccg ctgatgatag
      301 actccacgca aaccgacgtc atcgaggcgg ccctgaaact gtacggcggg cgcatgatcg
      361 tcaattccat caatttcgaa tccggcgaag aacgcgcaga aaccgtgacc gcgctggcgc
      421 gggcctacgg cgcagcgctg gtggctctca cgattgatga aaccggcatg gccagaaccg
      481 ccgacgagaa attccgcatc gcggagcgcc tggtaaaatt ctgcgaagcg cgcggcgtgt
      541 cccgcaccgc cctcttcatt gatccgctga ccttcacggt cggcagcgga gatgaaacgc
      601 tccgcaccgc ggcggtcgaa acagttgcgg cgattcgccg catcaagaag gatctgcccg
      661 gcgtacggac cgttctcggc ctttccaata tttctttcgg cctgaagccc gccggacgaa
      721 aggtcctgaa cgccgtgttt cttgaccgct gcgtcaaggc cggcatggat gcctgcatta
      781 tcaatgtcgc ggggattgtc ccgcttacag agatttccgc ggacgccctg aaggttgcag
      841 gggcgctgct ggacaacgac cagtccgcgg gcgatccgct tgagaatttc atcaacttct
      901 tcagcgacga gcaggcggaa gacgcttccg ccgccgcggc cgcaaaagag cccgaggaca
      961 tcctgaccga agcggtggtg cgcggccgcc ttcagccgct gggcgatgtc atgcccggcc
     1021 tgctccagaa atattccgcc gagcatattc tcaacgaaat cctcgtgccc gcgatgcagg
     1081 aggtgggccg cctgttcaat gacgggattc tgcagcttcc gtttgtgctg aaatcggccg
     1141 aggtcatgaa aaagtcggtg gatctgatca agccgcacat gaagaccagc gacaccgggg
     1201 cgcgccgggc cacgatggtg ctggccaccg tggcgggcga tgtgcatgac atcggcaaaa
     1261 accttgtgga catcattctt tcgaacaacg gctttcatgt ggttaatctc ggcaccaaga
     1321 tgccggtcga gcagatgatc gccgcggtga aggagcaccg ggtcgacgtg ctgggcatga
     1381 gcggcctgct ggtgaaatcc gccgcgcaga tggccgaaaa catgaaggcg ctggatgcgg
     1441 ccggacttgc gatacccgtt ttcctcggcg gggcggccct cacggcggaa tacgtcgcca
     1501 acacctgcca gccagcctat tccggtccgg tgatctactg caaggacgct tttgaaggcc
     1561 tgacgcggat gcaggagctg gtttcaaaag gaagactgga gaaaaccccg cccatcaaac
     1621 ggccggcggc cggacccgcg gaactgcgca cggcgccctc cgaaccggtt gaacagattg
     1681 agcccccgac gccgcccttc tgggggcacc gcatcaaccg cgacatcaat ctggaggacg
     1741 tctatccaat gctgaacgag aaatccatcg tgcgcggcca gtggaagttc cgcaaaggaa
     1801 aactgacgga ccgggaatgg cagaagctga tggatgaaga ggttaatccg cggatggaag
     1861 aactgaaaaa gaagttcaga aaccgggatc ttttccagcc gcagacggcg tggggatttt
     1921 atccctgccg gtcggaaggc aacagcctgt ttgtatttgc cgatgaaaaa tcggagccca
     1981 tccgccttga ttttccccga cagcgcaagc ccccccacaa ggccatcccg gactatttcc
     2041 gcgccgaccg cgacgtggtc ggcttcatgt ccgtgacgct gggccggggc cccgacaggg
     2101 aaacgcagcg gcttttgaaa gaggaccgct atcaggatta ctatctgctg tacggctttg
     2161 ccgtcgaact gaccgatgcc cttgcggaat actgccacaa aaccatgcgc cttgcatggg
     2221 gctttgatga gccggagatg gatctgcagg gctataacga ccagctctac cgcgggtcgc
     2281 gctacggctt cggctatgcc tcctgcccgg accttgccat gaaccgcatt gcctgcgacc
     2341 tggtgcatgc gaaggaaatg ggcgtggagg tcagcgaaaa cctgatgatg attcccgaat
     2401 tcaccacggc tgcgatcgtg cttcaccatc cgcaggccaa atatttcaac gtctaaccgg
     2461 agatccgcat gcgtcacctg aaaaaactat ccgagattga ctggaaaaca tggaaaccga
     2521 aggagcgggc cacgctcctg tttgtgatcc gcgacgggca ggcgcttctg attgacaaaa
     2581 agaaggggct gggcgccggc aaggtcaacg cgccgggcgg ccgggttgaa aagggggaaa
     2641 ccgcccagga ggccgccatc cgcgaggtgg aggaggagct ctgcgtcacg ccgctgaacg
     2701 tcagcccccg cgggcggctg aattttcagt tcacggacgg ctactcgctg cagggccatg
     2761 tgttctgggc caccgactgc cggggcgaac cgcaggagac ctgcgaggcg gtcccgcgct
     2821 ggacgccgct cgacgaaatt ccctacgaca aaatgtgggc cgacgatctg cactggattc
     2881 cgctcatgct ggagggcaag cattttgagg ggcatttcat cttcgatgac gacaccatgc
     2941 tggatcacac tctcatcacc ttcacccccg aagaatgggc cattgaatgc aggaatggat
     3001 ctggagagat gtaaggctgg agatcccggg cgactgggag atgcttcagt tctcacgcgc
     3061 gcgcgacaag ggccgttgcg catatgccga tcgcaggcag ttccggctgg agctgagctg
     3121 gcggcaggtg aaaggcgcgc cggattttcc ccgcatgatg gcggactaca aagcccggct
     3181 cgaatccgcc ggtgaacttc aggatgcaca cacctttaca ctgtccggct ggcacgggct
     3241 cgcggggatg cagaagaacc tgcgggtctc ccgtttcggg aattatttcg acaaagaaaa
     3301 cctccttgcc gaacttgttt ttctctggcc ggacaagagg gatatcccgc tggaaaatga
     3361 tattcttcag tcctgccgtt atgcgccgcc tgaaaacgga ttcagaaaaa tcaaagcatt
     3421 cggcatgtcc tgccgctccc ccgaaaaata tgaatttacg gcctgcagtg tgcagcccgc
     3481 gatggccgcc atggtgttca gcggggcccg ccggccggcg cgcatcaggc tccagcgcct
     3541 cgggatggtt ccgcaatggc ttgatcagcc gctgaccacc tgggctggaa cgcagctccc
     3601 gaaggaggcc cggaatcccg actttgaatc ccgcagccgg aatggacatg aaatcgtgac
     3661 ggcaaaagcg gattatgccc ctcgcggatt tctgatccga aagggcctgt acgctgcaag
     3721 cgcatggatt tgtccaaagg acggacggct ttatcatgcg gagcagatct gtcgttcagc
     3781 aaaggatatc gtgaaccacc cgctggactg ctgcggaggc atgtcatgat ggcgggattc
     3841 tccaatcctt cgtggtccga catgctggat gcgctcatga tccgcaaccg cgccgcgcgg
     3901 gtggagcgcg acgcgcgcag cggagaaatc cggattgccg ttcccacgcg ccggccgtgg
     3961 tatcatgttc cgccggtgac gtggatcgtg cgtgcgccgg aggagcgcgt cacggttctg
     4021 gatgaacccg gaagccttgt ctgggaaatg tgcgacggcc gcaccaccgt cgagaatatc
     4081 atcgacgcgt tcgcggaaaa atacggcctg acctttcacg aaagccgcgt ggccgtcacc
     4141 cagcacatca aaagcctcat cagccgcggc gtgctcgcgg tggaatttcg ggacccgccg
     4201 gcgccgggtc tctgaggtcc cgggaatata gtactaattt agtactatat acttccccgg
     4261 aagaacatcc cccgccgggc ccgttccgcg ccggtcagat atccagcagt tcgcggacat
     4321 cctcccacgt caggctttcc atcatctgac cggccggagc gagggtggcg ttgatgacgg
     4381 cctttttgcg cttttgcatt tccagaacct tttcctcgac ggttccgtgg gtgatgagct
     4441 tgacgctgta gacggtgcgc ttctggccga tgcggtaggc gcggtcggtc gcctggtcct
     4501 cgaccgcggg attccaccac ggatcgtagt gaatcaccat gtcggcgccg gtcaggttca
     4561 ggccggttcc gcccgctttc aggctgatca ggaaaacagg gatgtcgcgg ttggtgttga
     4621 actcgcggac gacgcccatt cggtcctttg ttgcgccgtc cagatagcag taggttcgtt
     4681 cgcgcgcggc cagttcgcgc ttcagaatgg ccagcatgga cgtgaactgg ctgaaaacca
     4741 gcacgcgatg cccggcatcc agcgcttcat ccagcagttc gaagaaaagg tcgagcttgg
     4801 ccgacggata ccggctgtcc agatccttca gcttaagcag ctccagatgg cagcatatct
     4861 gccgcaggcg cagcagcgtc ttgaatattt ccattcgcga gctgttgaag ccccgggccg
     4921 aaaccattcc ggatattttt cggcgggact gctccagtat cttcttatac acgagctgct
     4981 ggtccgccgt gaggaggcag ggcgcgatgc gctcaatctt gggcggaagc tctttggcca
     5041 catccttttt caggcggcgc agcaggaacg gctggagctt gcggcgcagg ccggtctgcg
     5101 tcatgtccgc ctccgggccg ccgccggcaa tcggcagctc ataatgctcg cggaatgacg
     5161 gatgccggcc gagatatccg ggcatcagga aatccataat ggaccacaaa tccgccacgc
     5221 tgttttcaat gggcgtgccc gtgagcacca gccggtgcac ggcattcagc ttttttgcgg
     5281 ccagcgcatt ctgcgtggag cggtttttga tatgctgcgc ctcgtcaaga accgcgcacg
     5341 agaacgaaca gttgagatac tgctcgatat cgcggcgaag cagcgcatac gaagtgatca
     5401 caatgtcggc atgttcaagt ttatcccgtt tttcgtgacg ttcagccccg ctgagcgtca
     5461 gcacccgcat gtccggcaca aagcgtccgg cctcctcggc ccagttttcc acaaggctcg
     5521 tggggcacac aatcagcgcc gggcggccgg cggcctcgtc gtggacacgc ggcaggcgca
     5581 gccacgccag cgtctgcagg gttttcccca gccccatttc gtccgcaaga atgccgcaga
     5641 gataattgct ctccaggaac cggagccagt tcacgccgtc tttctggtag tgccggagca
     5701 cgccgtccag gttcccgggc agcgggaccg gctgaagcgg caccccgcgg ttctgttccc
     5761 gggccttttc gcgccacgcg ggtgccgctt cgacatccac cccgtccagc gagtcgaggg
     5821 aggcctggac ataggccgaa tagaggcctt ccaggcggaa aagcccgggg cggctcccct
     5881 cgcccgccgc gcagtctgta aacacatcgc gggcggtctg gatggcatcg gcatccagca
     5941 ggattgtgcg gccgtttttc tcgacgtacg catctccctt caggagcgcc cgctgaatct
     6001 ccgcctccga caggctctgg ccccgcgtgt cctcgtatcg gaaaccgacc tcaaaccagc
     6061 gcccctcctc gccgctttca atgctgatga cgggcgtggt aaaatcaagc tcatccatga
     6121 agggctccac gcggccggcc agcgccacgg tccatcccat gcgccgtagt tttggaagcc
     6181 cgctcccgag aaaattcagg acttcgcggc acccaataac cgcggccaga ctgtcgccgt
     6241 gctccccgcc gaagccgatg gcccgcagaa tttcaagagc ctccgcctca cgcgccggat
     6301 tccgcaccag atagcgcatg aggtcctcgg gatcgggcag cgcgaactgt cccgccgcat
     6361 ccgccttgcc ggcaggcagc cggtgttctc cgtacacggc gaccagctgc gcggacagtg
     6421 aggccgggct tccacggaca ttcagcgtga agcgcggctc tgccggttcg atggtgaaaa
     6481 gatcgaacgt cacatcggtc tccaccctga tgtgcgcctc gatcagggga agttcggtct
     6541 gcatgaagcg cggcaccgag gggcgggcaa tgcggacggg attgcgatag atgttctgaa
     6601 gcggggccgg cagcacattg ccgaggggcc agaactgccc ggcatcaaaa acccagccct
     6661 ctttccccga cacgatgtat gtcgggagac acgccgcatc catgaacggc agctccgtgt
     6721 gaatcatcag caccagttcg ccgctggcgg aatcaagatg cattcggatt acggagttca
     6781 tcttcacggc gttgaccgtc agcggatgat cggccgatcc ctcataaagc ggccgaccgg
     6841 caagcagttt gatcagattg atgaaatccg aagcctgaac actcaggcgg gcgggcaccg
     6901 ggccttccgc aatgtcttcc agcacaaaaa gcagattgtc gtcgcgccgg ctgacggcca
     6961 cgggcacaga aagatccatc tggtcagccg gaacgaggcc tccggaggtc ttgtagcaga
     7021 tggacagcgg gaacccctct gcacccgccg acggccactg ggggtccagg atcagcatgg
     7081 gccggcaggg gcgcgccccg gccgttcccg agggcacacg ccggagatac tgcgactcat
     7141 ccgtggacga aagccgctcc gctcggcggc gctcctcctc gtacttgcgc tcgcgctccg
     7201 gatcggtctg ccgcttgatc attatcagcg ccagcgcgat gacatgggcg cagatgattc
     7261 ccctttccgt gttgtcgcgg caggggcact ggctggcgac cgacccgtcc ttcagcagct
     7321 cgaactggca ggtcatgttg cggttgccgt agatcagcga gccgcgcaca aagggatgtt
     7381 cgtaggaaac atgttccacc cggccggttt cgcaaagcgt ctttccttcg cggaaaacgc
     7441 gcgcccccgc ccagtccacc agcattttct gtgttaccgg aaacctcata aacagtccgt
     7501 gcgcgggccc cattctcccc tgaatctggt gcgcccaaaa tatggaaaaa catactatga
     7561 agcgcccccg acccccaaga cattttttct ggctcgcaat cgcgcggcgg ggatgcgata
     7621 caggagtgaa aggaaatgac catggaaacc aagcgcgcac aggccatcaa gcatctcgag
     7681 gggcggaagt cactgccgat catcgaggtg ttttatgaat tcaaccatct caaatcgctt
     7741 taccgccagg gctggctccg cgtcggaatc cccagggaac actgcgagac cgtggccgag
     7801 cacacgctgg gcgtaaccct gctggcgctc ttcctcgcgg acgaatattt cccggaactg
     7861 gacaaatgga agctgatgct gatgggcctg ctgcacgact tcggcgagat ctacgccggc
     7921 gacatcgttc ccgggaagat gacgctggag gacaagcacc gcctggaaag aaaatcggtc
     7981 gaacaggtct tcgcgaggct cccgaagggg aacgactatc tggcggtctg ggaggaattc
     8041 gaggccggcc aaaccgccga ggcgcgcttc atgaaggaaa ttgaccggct cgaaatgggc
     8101 cttcaggcca gcgtgtacga gcacgagaag ctgggtgatc tttccgtctt ttttgattcc
     8161 accgatcgcg cgctcaaaac cggccggctg cgcgatattc tggatgcgct ccgcaggctg
     8221 cgcgccacgg cgccctgacc gcggatcaga tttctttgat cacccgggcc ggaacgcccg
     8281 ccgcgaggct gttcggcggg atcggtttgg tgacgacagc cccggcagcc agcacgcagt
     8341 gctcgccgat gcctgccgcg tccagcacgg tcacgcgcgc gccaaaccag cagttggggc
     8401 cgatcgtcag ttcgcccttt gtgttcgttc cgctctgctg cgcgaaggtg tgctccctgt
     8461 ccgtgtagtc gtattctcct ccgctcagaa aatagcagaa ggccccgatg accgtgtcgc
     8521 gcccgagcgt cagcttgttg gaggaaaaca cctggcagtt ggacgaaaga ttcacgttgt
     8581 ccccgatcgt gatactgccg tttttgcagt agatgatcgt gttgcggccc acatagacat
     8641 tgtcgccgat ttcgatgcct tcattggtct cgcccttggc gtcaacgacg caatggtcgt
     8701 ccagcaccac gttgtcgccg atttcaatct tgtgaggatg ccggatggtg atgttccggc
     8761 cgaaaacgac cttcctgccg atgtcctcga acatgaaggg ataggtcagt ttgcgcaacg
     8821 cgaggcccag cgcgccgggg atccacccga acagtgtgat gataacttcg cacaggatga
     8881 cacggccgac gctcatgtcg ccgtagtaga cggagcggta ctttttgaag gccgactgtt
     8941 tttcgtcctt catcacatcc ttgattttat attcctgcgt ttccatgccg ttcctccgtt
     9001 cagcgaatcg aggcaatgaa tgccttcatg gactccacgg tttcgccttc aaaatagagc
     9061 cggtaccaga tttccaggtt cagcagaatc cacagcctga aattgtggtc gcgttttccg
     9121 gaaagatgct catccagaag ccggtctaca taggcgccgt cgaaaatgcc cagctccaca
     9181 aacttcgact gcgcaaaaag atttttcagg aacgtgctca gctcgctccg catccacagg
     9241 gcaatgggaa aaccgaagcc ctgcttcttg cggtagatca gttcctgcgg cagatgacgc
     9301 gaggcgacct gcttcaggat gtgcttcatg tcccgcccct tgagcttcat gccgccggga
     9361 atggatgccg caaactccgt gaccttgtgg tcaatcagcg gcggtcgcgc ctccagcgaa
     9421 tgcgccatgg ccatccggtc cacgatcggc agcagatgat ccggcatgcg ggtcatcagg
     9481 tcggtgtaga gcatctggtc caccagttcc tccgcccggc cgcagttgaa atgcgtcagg
     9541 attttctgga atgaatcagc atcggcgatg cccgttttgg cggggccggt gaaaagcgaa
     9601 tccttgtgct ccggcgtgaa gcgcaggaag ctcgcgctct gcgcatagcg ctcgccgtgc
     9661 tcgtagaacg acatatcgtt cacccatgcg actttctggg cgaggctctt gtagccgaac
     9721 gattccggca ccatcgaggt gagctttttc atgaccgtgc gccggaacca ggcgggaagc
     9781 aggcagtaga aatccaccat gcgctggccc gcgaagcggt cgtagccggc gaagttctca
     9841 tcgcctccgt ccccgctcag caccaccttg acttcctcgg cggcgacctg cgaaaccaga
     9901 tagaccccca cgccgaaggg gtcggacggc tcgtccatgt ggtgaatcat ggatgggatc
     9961 aggttgatca tgtccgcctc cacaatgcgc tcgcgggcgg tcagccggta cttgtccgac
    10021 accatgcgcg cgtagggcag ctcgttgaat ccctgctcct tcacgccgat cgaaaaagtg
    10081 gggaaggctt cgccggtgat ctccgccatc agcgcggtga cgaggctgga atcaatgcct
    10141 ccgctcagga aggttccgac cggcacatcg ctgagcagat ggagcttcac cgtctcgcgc
    10201 agcagatgct ccagctcgtc ggtgacatcc ttctcgcttc cggaaatctt gtttttgaaa
    10261 tcgagcgtcc agtactgctc cgttttgact tcgcgcccgt cgaaaaagag cgacgtggcc
    10321 gccggcagct ttttgatgtt cttgaacagc gtgcggtcgt ccggaatgaa ccggagcgac
    10381 atgtagtgcc agatggccgt cagatccagt tccggtttaa caagcccgga ggcgaggatc
    10441 gccttgactt cggaagcgaa gagaaaaata tttccgtcga aataatagaa cagcggcttc
    10501 tggcccatgt gatcacgggc gagaaacaga gatttgtcgc gcgcattcca gatcgcaatg
    10561 ccgaacatgc cccgcaggtg tttcgtgcag tttgcgccgt attcttcgta aagatgaaga
    10621 gccgtttcca cgtcggtgtg cgtcttgaaa acgtgcccgc gcgcttcgag gtcgcggcgc
    10681 agctcggggc tgttgtagat ttcgccattg cacaccagct gcaggttgcc ctcttcgttg
    10741 cagatcggct ggtgcccgcc ggccacgtcc acgatgctca gccgccgctg gccgatcgaa
    10801 atggtgtcat cgtgataaaa accctcgtcg tccggcccgc gatgcttcat cgtgtccgtc
    10861 agcttcgaga tgatattccg gtggcccgga gccataacgc ctgcaatgcc gcacataaaa
    10921 ccgtccgttg ttagtcgaaa gccggatact agcttttcgc ccgcggcaat gcaactgggt
    10981 tggccggcga aaagttccaa tggttggaag tttttctgct gaaagttcca atgattggaa
    11041 cttttatggc ggcgcccttt caacccgata tacgctcacc acggttcggc ggcgatcatg
    11101 gcctcgaggc ggttctccac atggtcgggg aggttcggaa ggaaatccag accggtgagc
    11161 tgctcgatgc ggtctatgga ggtcagatag tacttcagct ccttggacag cgcctcgtgc
    11221 ggcatgaggt aggcaatagc acggatggcg ttgtcttttt catccaggat gattttgaag
    11281 aaagcgtccg ggatttccgg accgcccgga atcttctcga tcttctggtc gtacaccggg
    11341 ccggtaacaa cccatatttc accgaagttg aacgcgtagt tattgagctc ctggtgctcc
    11401 agctcctgcc acggaccgcc gttgaagggc accttctgag gcgtcatgtt ggacatcaga
    11461 taggcctggt tctgcgcttt tttcccgtaa cagacggcca tggcgaatga gggcgccatg
    11521 tgcccgcggg aatagcccga gttgctgtaa tcgttgttgg aaaccttcgc ggacgttctc
    11581 atgtccgtgt tgtagcggtc ccggcggctg gtgtccggac ggttccgaac acgatgcagg
    11641 cggtaggcca cccacgccgg attgcggcgg gcttcgtcgt acccgacggc atagcccctg
    11701 ttcttgagca gcgtgatttt ctcaaaccgg ttcgaggccg caaccggagc cccggcatag
    11761 tgatagggat cccgctcggt ctgcgcgcag gcggaaagcc caagcatcat cagggtgacc
    11821 acaatgaagc ggcggctctt cacgcgggcg gcctcatttc aatgatgacg ggcgaacccc
    11881 acaggcgggt gagccccagc gcgcggcagg cgccctcgag aaaagccgaa aggccgcggc
    11941 acttcagcat gcggtgcagc accatcggca ggaagaactg ccccctgcgc gcgtgaagat
    12001 gccagccgct gtcccgaaac gcatccatga tttgcctgtg ccggaaaaga atccatgtcc
    12061 gggtgttggt ttcgattttc ttcttggcgt tgaacagggc cggagccacc gcgttgaggc
    12121 tctggctggt cggatagtcc acaatcaccg cgctcttcgc gacgcggcag agctcccgga
    12181 tcagcaccgg ccactgctcg cagtgcgtga ccatgcgaaa cgcgatgacc acatcgaaac
    12241 tgttgtcggg aaacggaagg gcaatgacat tgccgaccag gaacctgcac tgcccggaat
    12301 ccacgatttc gcggattcgc tttttgcagg actcatcgct gctcaggacc gtgacgtcat
    12361 atccggcttt gcacatcggg atggcaagct gtccgtggcc gccgccgaca tccagcacgc
    12421 gcgcgccggg atggccgcgc aggaaccgca tggtgatgcg ctcctgcacc tccagcagcc
    12481 actgcccggc cgcgccggca aagcgcccgg cgtaatcgtc ggacgaggtc tcaatgtccg
    12541 ctgtttctgg aaatacggcg tctgcttcct gcatttcagc tgaatcctgt tgtctcctgt
    12601 gacagtcgtg ctttatagtg cccaatcaga gaagcataag caagagtgta aaaaaatgcc
    12661 gatactggcc gatacgcata ttcatgtcta tccgttctac gagctgtcgc tgtttttcgg
    12721 cggcgcattc cggaagtttg cggcgctgga agaaggtgcg gaccatgtac tctttctcgc
    12781 tgagcggcat gactgccatt tcttccggca gctgaaagag gggctctcca aggtttccgg
    12841 aatggaatgc gcggccggcg aggaagtttt gaccatccgc aaccggaacg ggaagcgcct
    12901 ctggctggcg gccggacggc agatcgtcac ggcggagcgg ctggaaattc tcgcgctgac
    12961 caccgatgcg gaaattcccg acggtcagcc cgcgcgcgat gtgatccgca gaattcgtga
    13021 aatcggcgcc gtccccgtga tcgcctgggc ccccggcaaa tggtttttcg gacggggaaa
    13081 agttgttcgg gcgctgattg attccgaacc cgcgggcagt tttcttttgg gcgattccac
    13141 gctgcgcccg accgtctggc cggagccgct gctgatgcgc cgcgcgaaaa agaagggatt
    13201 caaagtggtg gccggctccg atccgcttcc ggtgcccggc gaggaagcaa aggcggtttc
    13261 gtacgggatc cggtgcgacg ggaacattga tccggcgcag cccttgaaat cgctgcggga
    13321 catgctgaca aacctgcccg gcgcgaagaa tttcggccgt cgcgattcgc tgctgagtgt
    13381 tttaacccgg ctgaaaaaga acagctcggc aaaatcatga acgacaaaat gcgcagagtt
    13441 cttttcatat cgccgcagcc gtattttcag tggcgcggct cgcccatccg cgttgcgttt
    13501 gacgcgcagg cgctgtccga agccggatgg atggtggatc tggtggtaat gccggtgggc
    13561 gaagaccatc cactgccgaa tgttgccgtt catcggccga tgaatatttt tagaatcaat
    13621 aaactggcga tcgggccgtc cgccgtcaaa gcgttcctgg atattttcct tttctgcacc
    13681 gcgctgaagc tggccttcaa aaaccgctat gacatcatcc atggcgtgga agaggccggc
    13741 gccatggctg ttgtgctcgg gaaacttcgc ggggcgaagg tgattttcga aaagcattcc
    13801 gaccccgcat cctataaaaa ggggtttgtc cgcaatgcgg tcatgtggct ctattcgcag
    13861 gtggaaaaat tttcgatccg ccacgcggat gccgtcatcg gcaccggccc cgcgctggtc
    13921 gagcaggcaa ggaaaatcaa tccgcgcatc accgcgcatc atattttcga cattccttct
    13981 tcgctggtgg aagccgtgcc ggaaaagatc gccgccgcgt gcgccaaact ggagcggaag
    14041 cccggcgacc ggatcatcac ctatgtcggg tcgtttgcgg tgtatcaggg cattggcctg
    14101 atgtttgaaa gcattccgcc ggtggtgcag gcccggccgg aggcgcggtt tgtcatcatc
    14161 ggtggaacgg aacaggaaat tgccgaacgc agagcgtggc tggcggagag gaagtcggag
    14221 catgcggtca tgttccccgg taaaattccg ccggacgagc ttccgaatta tttatcagcc
    14281 tcggacatac tgctttcacc gcgaacttct ggcaccaaca cgccgctgaa acttctggat
    14341 tatttgaagg ctggccgcgc catcgtggcc tgcgacattc cggccaaccg cctgatcgtg
    14401 gacgaggaga ccgcgctgct ggtgccggcc gatccggatt cgttttccaa gggcatcatc
    14461 aagctgctgg acaacgatcc gctgcgcgcc cggcttgcag gaagcggccg ccggcttgtg
    14521 gatgaaacct acaacttccg cgt
//
LOCUS       DAOQWR010000055        14405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4673_length_14405_cov_4.677422, whole genome shotgun sequence.
ACCESSION   DAOQWR010000055 DAOQWR010000000
VERSION     DAOQWR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14405
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4673_length_14405_cov_4.677422"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKK36_06005"
     CDS             complement(<1..196)
                     /locus_tag="PKK36_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94149.1"
                     /translation="MKGKRFDILEETKANKNYATIDQIITIYENAARMRGLSDKTIYD
                     NIRSFHQVLGLQTGSKASSQI"
     gene            528..604
                     /locus_tag="PKK36_06010"
     tRNA            528..604
                     /locus_tag="PKK36_06010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Arg,seq:acg)
     gene            complement(611..1933)
                     /locus_tag="PKK36_06015"
     CDS             complement(611..1933)
                     /locus_tag="PKK36_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNR94150.1"
                     /translation="MSSIFKAYDIRGIYGDTLSDDLAYKIGRAYVTFLKCKSVVVGRD
                     MRPHSVPLFKALTNGIRDQGADVVDIGMCSTPMSYFANGSLKAEGSIMITASHNPGEW
                     NGFKLCRENAIPISGITGIKDIEKIVQNETFAPKAEKTGTVSTHNVTAEYVKHIQSYA
                     ALKRPVHIAIDYANGMGIEEGKVFEGLLTADSLFDTLDGNFPNHEANPLHLETFEPLQ
                     SMVRKGKYDFGVYFDGDADRAGFCDENGDIVSMDMITGLIAEAMLKRDGGGAIFYDLR
                     SSWAVKELIEENGGTPMMSRVGHAFIKKQMREADAVFAGELSGHYYFKDNFFTESSSL
                     AVIYVANLVSQSGKTLSELIKPIRRYFASGEINSEVADSKALFEKLKRKYSGGKVIEL
                     DGLSIEFDNWWFNVRGSNTEPLVRLNMEARSKALMEEKRDELLAIIRG"
     gene            2056..3108
                     /gene="mnmA"
                     /locus_tag="PKK36_06020"
     CDS             2056..3108
                     /gene="mnmA"
                     /locus_tag="PKK36_06020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNR94151.1"
                     /translation="MNSRVRVAVGMSGGVDSSAAAALLLDQGHEVVGLTAHMWKGSRC
                     CSLEDVDRAATVCRHLGIRHEMVNAVEEFGHDIIEPFVRDYAEGRTPSPCVTCNERIK
                     FGLCLAAGRKLGCEYFATGHYARIENRSGSYHMLRGHDPEKDQTYFLHRLSQEQLAHI
                     LFPLGGMRKPEVTAYVKARGLPALFRGESQDLCFVPDDGHGAMIERYRKELKKPGDIV
                     KRDGRVLGVHGGFYNFTIGQRKGLGIAWTEPLYVSDVRPEANQVVVGPRSEVMRDACR
                     LHDIHWMNEVPVSPLHCDVQIRYRHHAVPAKVCFDGNRAVVQFSEPQFAITSGQAAVF
                     YSEKEVLGGGWIEVVL"
     gene            3123..3902
                     /gene="larE"
                     /locus_tag="PKK36_06025"
     CDS             3123..3902
                     /gene="larE"
                     /locus_tag="PKK36_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNR94152.1"
                     /translation="MKKLRECLRDTGAVAVAFSGGLDSTLLAAVAFQELGSRAVAFTV
                     HSIFISEAERDDADNLAREIGIRHVILPMDVTTVPEVMGNSPDRCYHCKFHVFSNIVK
                     HAAKLDIRTVVDGSNADDLNDFRPGSRAVAELGIRSPLKELGFTKDDIRAASASLGLS
                     SANKPAMACLASRIPYGTPISPDALKKVERAEDILRRGGFHQFRVRAHGNVARIEVER
                     DEIRTLQEKSGTIIPAIKACGFDQVSVDSEGYRQGSLNPKS"
     gene            3899..4624
                     /locus_tag="PKK36_06030"
     CDS             3899..4624
                     /locus_tag="PKK36_06030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNR94153.1"
                     /translation="MNIILLEQEEVRDGITVLDDDRAGHIRKVLHLKEGDSLRIGLVN
                     GPRGQGTISEIKKHAVRLRCEFENEMPPESRIDVLLALPRPKVLKRLWAPLASMGVGH
                     VVLTNAWKVERNYFDTHVLEEKYYRPRLIEGLQQAGDTHLPDVHLFRTFTEMMERLDE
                     MFPASLRLLADPSGRISVHDAVLKSKPDRILLAVGPEGGWIPQEIESLCAKEFQLAGM
                     GPRILRTDVACISLLALANDALE"
     gene            complement(4710..6392)
                     /locus_tag="PKK36_06035"
     CDS             complement(4710..6392)
                     /locus_tag="PKK36_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNR94154.1"
                     /translation="MSENETAAPLHFIRQIIADDLKSGKHTHIVTRFPPEPNGYLHIG
                     HAKAICIDFGMALENNGTCHLRFDDTNPTAEEVEYVESIMEDVRWLGFDWGTHLYYAS
                     DYFDQMYEFAVQLIKKGKAYVCTLSAEEFKEYRGVPTRPGKESPWRNRPIEESLDLFE
                     RMKAGEFEDGAYVLRAKIDMNSPNLHMRDPAIYRIKKAHHHRTGDKWCIYPMYDFAHC
                     IEDSLEKITHSLCTLEFEVHRPLYDWILEELDIYRSRQIEFARLNLTYTVMSKRKLLE
                     LVEEGLVKGWNDPRMPTISGLRRRGYTPESIRDFCERVGVTKTESLTDVALLEHCLRD
                     DLNKSALRRMAVMNPLKLVIDNYPEGKVEEFDAVNNPEDPAAGTRKVAFCRELFIERG
                     DFMENPPKKYFRLSPGQEVRLRYACIVKCTHVVKNAAGEVTEVHCTWDPDSRGGSAPD
                     GRRIKGTIHWVSAKHAVETEVRLYDRLFSVEDPMDAVEGKDWKDHLNPNSLEETTCLI
                     EPALVKAEPGTRFQFERIGYFCVDSVDSKPGRPVFNRTVTLRDSWAKQGARE"
     gene            6525..7847
                     /gene="gltX"
                     /locus_tag="PKK36_06040"
     CDS             6525..7847
                     /gene="gltX"
                     /locus_tag="PKK36_06040"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNR94155.1"
                     /translation="MRKIRTRFAPSPTGHVHIGNIRAAIYNWLFAHHNGGQFLLRIED
                     TDTERSTPEAVQTVLDAMDWLGLTYDEEPLYQSTQRAKHLEAAEMLLSKGLAYKEDKG
                     GTGKGECIIFRMPGRDMTFHDLVKGALTKKAGDMKDLVIVRSDGSPVFHLANIVDDIT
                     MNVSHVIRGDDHVENTYRHVAIYEALGAEVPQYAHLPMIVNAQGKPYSKRDGAAYVGE
                     FRDREYLPEALFNYLALLGWSPGDDREIMSREEMVELFDLARAQSSPAQMDLKKLEWM
                     NGVYMRSQPREELEKRCREVMMQRGLWDSATAPDYFSAVLRAMGDRIHFYSDIALHAG
                     FFFTENYEYDEKAVAKRLMKEGALDELREMGKRFAASDKFDAPSLDKILHDYAAELGA
                     NAGQLIHPVRIAVSGMAVGPGLFEMLEVLGRERVLARIERTLRKFGGA"
     gene            7857..8492
                     /locus_tag="PKK36_06045"
     CDS             7857..8492
                     /locus_tag="PKK36_06045"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR94156.1"
                     /translation="MGDSSAGCPRLTLATRITLFRILCVPVYILLLIYYQMGLKQGAA
                     NEYFRSGALLVFILLAASDFLDGYIARTRNQVTRLGTLMDPVADKLLMISSLILLTRP
                     SLPALQPQFPVLIAVLFISRDALLVAGAFVVQHFSGEVQVKPRWSGKASTAAMACCVV
                     YILLGWPAHWFQPLLAVAGMLIMLSLVQYLLDGIRQLEGPAINRPEKEHHA"
     gene            8485..9843
                     /gene="der"
                     /locus_tag="PKK36_06050"
     CDS             8485..9843
                     /gene="der"
                     /locus_tag="PKK36_06050"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNR94157.1"
                     /translation="MPENKGQQRVVAIVGRPNVGKSALFNRLVGRRVAIVHEESGVTR
                     DRLMSEAVWDEEHFELIDTGGVGMMDGSRTGSTIEDGTRQQVDLAIQDAAAVIFVVDI
                     TAGVLPLDREVARLLHASGRPAFVAANKADHQNLQNQADDFAELGFPVYPVSALHNRG
                     VDDLMSGVLPALPAPVPRTAIQPLKVAVVGRPNAGKSSYINRLLRSNRVIVSEVPGTT
                     RDSIEIPFAIGSGNQARHYRLIDTAGIRKTSKVNTAVEKFSVIRAENSIREADVVVIV
                     MDATEGPKKLDKRIASLAISENKGCVVLVSKWDLAEGTTTQRAYGKALREAVPFLNFA
                     PVVFASAKTGYNIRNTIEVIDHVAAQLDLKLTTGLLNRVIQDSVAKVAPQVVGTRRLK
                     IFYATQVGTRPIRIRFFVNSPRRIKENYKTYLLAALRKAFGLEGAPVILQFRARQQKT
                     EQ"
     gene            complement(9848..10150)
                     /locus_tag="PKK36_06055"
     CDS             complement(9848..10150)
                     /locus_tag="PKK36_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94158.1"
                     /translation="MNANRLAVLLSTLVYPGAGQLIQKRWAAGAVFGIAATLSLVFFL
                     AYATVILREYYRFAFEFEAREMPPLPLMRMMIAFLTTLLIYIAGIMDTVRANRKRN"
     gene            complement(10189..>14405)
                     /locus_tag="PKK36_06060"
     CDS             complement(10189..>14405)
                     /locus_tag="PKK36_06060"
                     /inference="COORDINATES: protein motif:HMM:NF028070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNR94159.1"
                     /translation="HVSRFWHNHQPTYWPEWNSNGDQNSRVQYAWDSIVLKPGQNYGG
                     ISPAYHPENDLGNIFGLDDRRNAYQYGPKNSLSTFSGGGFALSYSGSLIDNVRQLGGG
                     GHLGYGGDWNSGNTEARGWGRLDLVGFTYHHSLAPLLPKEVFRKELQIFKQAWWKAWG
                     GNSDLSDHSKGFFPTEMAYSRHLVDVLVDEGYEWTIVPSHHISRTCPTYFNYANPTGT
                     YNIYSSPPNKADLLGPSPTSGWWYSEPNPGNAAWNVAPYAYQLHKVQYVNPDTGATKE
                     MYAVPSDDVLSYRFGYANEGIGKINDNISPYATDPNRPVIVMPSSDGDNAWGGGSSSW
                     MEATPQFFNDSASAGYNKNTPQGFVDAAKANAPVAHIEDGAWIFPEMDYGSPNFMKWI
                     EPPLYNTAINATNRYDSTQADLVTPGFALKFFSYAPLMAGANWCITAEQIMRDQGGDV
                     QAWKIQAPYDWNGSWTSPNDVELAWHIYLTGLDSGFNYYGGLGNDDENKPALATKRAI
                     DKLQSYMSTRMHLDQTGPTVLKPQRFPYNPGAYTFGWFNYIPGGDTRYLKKMPSEFYI
                     WTHAYDVNGITNVSVRVRLDNDGTNSLSTTHNETFAGGADVGGWMTVAMTKRELPKTR
                     SALNTAAANGQIDYFVFDPAFWSNPVIADYYFVEINDGNVPGFRGKLLDYYVEAYDSL
                     GNVHKSEIQHVFVEHDGGQSSVYPSAAFDPAAPSDCASITVNFNAATSPLATAATVNV
                     FYHFSTNTNDWASAAMGRTSSNTFSHTFTTNDIPDNAPQLEVAFNDGTNWENNNGANW
                     KVPIRDCDAPVGPSSVVFDPAAPDGCDPVTIRYYPNEGPLEEASAVKIHIGRNGWQDV
                     ISPDPSMSQNGYYWEYTYIMETNTTEINVVFNNGAGVWDNNSGADWSVAVSNCGEEEA
                     PLPPVSISPTNPVGCGPITITYNPAGRVLLGADPVRIHIGRNGWQDVILPNPSMTKSG
                     AYWTYVYTPPAGSTNINMVFNDGGATWDNNGGADWHFAVEGCEDLPTGLAITNPPSNI
                     TVPNSTETYDLSGIADGMEGVLSWSNTLTGASGTLPAAATWSLTNVALASGGNEIIVR
                     GTNSAPPGVVTSAVDSASDAAYSDGWNSGNNGGTGFLGWSFNHTQDGETSWAGAFIAD
                     PANTGISGLGDTAFGFYANPFGSGANAEAIRNFSSPLGIGSTFRFDFGLNWDSNNEGS
                     NRGFNLLAGDTELININMGNTQTITINGETLFSEYGTQAMPLSFEYVADGSIRVRGTG
                     RNGLESYDQTLSVSTGAPSRIKFYFNATDSSDERQMYFNNLAITSPTAGGIQTNTDSV
                     IITRTGTPILDAIEAQEGGRFALTIGSTIFGAEYAVYETPILVPTQNWQIVAGSAMTG
                     TGADLEMVITNMVSTSAYYRIGYTLP"
BASE COUNT         2859 a         3812 c         4461 g         3273 t
ORIGIN      
        1 tgatctggct tgatgctttg cttcctgtct gcaggccaag gacctgatgg aaggagcgga
       61 tgttgtcgta gatggttttg tccgacaatc cgcgcatgcg ggcggcattc tcgtagatcg
      121 tgatgatctg gtctatggtc gcgtaattct tgttggcttt ggtctcctca aggatatcaa
      181 agcgttttcc tttcatggct ttgatcaggt cgcgggcgcg gcatttggca agattccgat
      241 caccagttcc aagagagcga aaataacgct tgccatcaat catcactcta aggtaatagt
      301 atcgcccacg tttttgaatg ttctttgact gagacgaata ttgcatagtt tttgctcctg
      361 gctgtatatg gatcatttgt atctgatgcc atatatgata ccaataaagt tggaaagcaa
      421 ggataatcat tgataagcat tgataagcat tttccaaatg gaaaatcagg aaaaactatt
      481 gaaaatattg agaaatatgc aatatttctc aggaaaataa cgaaagcgta cccttagctc
      541 aattggatag agcacctgac tacggatcag gaggttgcag gttcgactcc tgcagggtac
      601 gccagctttt tcagccccgg atgattgcca gcagttcgtc gcgcttttct tccatcagcg
      661 ctttgctccg ggcctccatg ttcaggcgga ccagcggctc ggtgttggag cctcgcacgt
      721 tgaaccacca gttgtcgaat tcgatggata gcccgtcgag ttcaatgact ttgcccccgg
      781 aatatttcct cttcagtttt tcgaacagcg ccttgctgtc ggcgacctcg gaattgattt
      841 ctccgcttgc gaaatagcgc cggatgggct taatgagctc ggacagcgtc ttgccggact
      901 ggctgaccag atttgccaca tagatcaccg ccagcgatga gctttcggtg aagaagttgt
      961 ccttgaaata gtagtgcccg gagagttcgc cggcaaaaac ggcgtcggct tcgcgcattt
     1021 gtttcttgat aaacgcatgt ccgacgcgcg acatcatggg ggttccgccg ttttcctcga
     1081 tcagctcctt gacggcccag ctgctgcgca gatcatagaa aatggcgcct ccgccgtcgc
     1141 gcttgagcat ggcctcggcg ataagccccg tgatcatgtc catggacacg atatccccat
     1201 tttcatcgca gaatcccgcc cggtccgcgt cgccgtcaaa atagaccccg aagtcgtatt
     1261 ttcctttgcg aaccatactc tgcaggggct caaaggtttc caggtgaagg ggattggctt
     1321 cgtggttggg gaaattcccg tcaagtgtgt caaaaagcga atcggcggtg agcaggcctt
     1381 caaagacctt gccctcttca atgcccatgc cgttggcgta atcaatggca atatgcaccg
     1441 gccgcttcag ggcggcatag ctctgaatat gcttcacgta ttcagctgtg acattatgtg
     1501 tggaaaccgt tccggttttt tcggctttcg gggcaaatgt ttcattttgc actatctttt
     1561 caatgtcttt tattccggta atgccgctga tgggaattgc attttcacgg cagagcttga
     1621 agccgttcca ttcgcccgga ttgtgcgagg cggtgatcat gatgctccct tcagccttga
     1681 ggctcccgtt ggcaaagtag gacatgggcg tgctgcacat gccgatgtcc acgacgtctg
     1741 caccctgatc ccgaatgcca ttggtgagcg ccttgaacag cggaacggaa tgggggcgca
     1801 tgtcgcgtcc gaccacgacc gatttgcatt tcagaaatgt gacataagcc cggccgattt
     1861 tatatgccag atcatcggaa agggtgtctc cgtaaatccc gcgaatgtcg tatgccttga
     1921 aaatactgct catagtgcat ctcccgtatt gaacatgtgg gggaatataa tcaattcaac
     1981 cctgcctgtt caaggttcat catacaatgc tggaaacttg caggctgggg cggtatgttg
     2041 tgcgggatga gtgatatgaa cagcagagtg cgcgtggcgg tgggaatgag cggcggcgtg
     2101 gacagttccg cggccgccgc gctgctgctg gaccaggggc atgaagtcgt tggcctgacc
     2161 gcccacatgt ggaagggctc gcgatgctgt tcgctggagg atgttgatcg ggccgcgaca
     2221 gtttgcaggc atctggggat ccggcacgaa atggttaatg ccgtggaaga gttcggccat
     2281 gacatcatcg agccgtttgt ccgcgattac gcggaaggcc gaaccccgtc gccctgcgtg
     2341 acatgcaacg agcgcattaa gtttgggctt tgcctcgccg ccgggcgcaa actcggatgt
     2401 gaatattttg ccaccggcca ttatgcccgg atcgaaaacc gttccggatc ttatcacatg
     2461 ctgaggggac acgatccgga aaaggatcag acctacttcc tgcaccggct ttcgcaggag
     2521 cagcttgcgc acattctttt cccgctgggc ggcatgcgga aaccggaagt gacggcttat
     2581 gtgaaggccc gcggccttcc cgcgcttttc cgcggcgaaa gccaggatct ctgctttgtt
     2641 cccgatgacg gtcacggggc gatgatcgag cgctaccgga aagagctgaa aaagcccggc
     2701 gacattgtga agcgggatgg ccgtgtgctc ggcgtgcatg ggggattcta taacttcacc
     2761 atcggccagc gcaaggggct gggcattgcg tggactgaac ctctttatgt gagcgatgtg
     2821 cggccggaag cgaatcaggt tgtggttggg ccgcgttccg aagtgatgcg cgacgcctgc
     2881 cggctgcatg atattcactg gatgaatgaa gtgcctgtat ccccgcttca ttgcgatgtg
     2941 cagattcgct accggcatca cgccgtgccg gcgaaggttt gttttgacgg caatcgggct
     3001 gtggttcagt tcagtgagcc gcaatttgcc ataacgtccg ggcaggccgc ggttttctac
     3061 agcgaaaagg aagtgctggg cggaggatgg attgaggtgg tgctgtgagt attgaagtca
     3121 aaatgaagaa actgcgggaa tgcctgcggg acacgggggc tgttgcggtt gctttcagcg
     3181 gagggctgga cagcaccctg ctggccgctg tggcctttca ggagctgggc agcagggcgg
     3241 ttgccttcac ggtgcattca attttcattt cggaggcaga gcgggatgat gcggataatc
     3301 tcgcccgcga aatcggcata cggcatgtga tccttcccat ggatgtcacc accgtgcctg
     3361 aggtcatggg gaacagcccc gaccggtgct accactgcaa gtttcacgtt ttttcgaaca
     3421 ttgtaaaaca cgcggccaaa ctggatattc gaaccgttgt ggatggctcc aatgccgatg
     3481 acctgaacga tttcaggccg ggcagccggg ctgttgcaga gctgggcata aggagcccgc
     3541 tgaaagaact cggtttcacg aaagacgata tccgggcggc ttccgcttct ctggggctgt
     3601 cctccgcaaa caagcccgcg atggcctgtc tggcttcgcg tattccatac ggaacaccga
     3661 tcagccctga tgcgctgaag aaagtggagc gggctgaaga cattctgcgc cgcggggggt
     3721 ttcatcagtt ccgtgtgcgt gctcacggga atgtcgctcg aattgaggtg gaacgcgatg
     3781 aaatcagaac tctgcaggaa aaaagcggaa ccatcattcc ggccataaag gcctgcggat
     3841 ttgaccaggt gtccgttgat tccgaaggat accggcaggg aagcctcaat ccaaaatcat
     3901 gaatatcatt ctacttgagc aggaagaagt cagggatggg attaccgtcc tggatgacga
     3961 tagggccggg catatccgca aggtcttgca tctgaaagag ggggacagtc ttcgcatcgg
     4021 acttgttaat ggccccaggg ggcagggaac tatctcggag attaagaaac atgcggtccg
     4081 gttgcgctgc gaattcgaaa acgaaatgcc gccggaatcc cggattgatg ttctcctggc
     4141 ccttcctcgc cccaaggtgc tcaagcgcct gtgggctcca ctggcttcga tgggcgtggg
     4201 acatgtcgtg ctgaccaatg cgtggaaagt ggaacgcaat tatttcgaca cacatgtgct
     4261 ggaggagaaa tactatcgcc ccaggctgat cgagggcctg cagcaggcgg gcgacacgca
     4321 tcttcccgac gtgcatctgt tccgcacttt cacggagatg atggagaggc tggacgaaat
     4381 gttccccgca tcgctcaggc ttctggccga tccgtccggc aggatatcag tgcatgatgc
     4441 tgttcttaaa agcaaaccgg accggattct gctggctgtc ggccccgagg gcggatggat
     4501 tccgcaggaa attgaaagcc tctgtgcaaa ggaatttcaa ctggccggca tgggtccccg
     4561 cattctgcgc acagatgtgg cctgcatttc tctgctggcg ttggcgaatg atgctcttga
     4621 atagaaaagt tccaatcatt ggaacttttg gtctgaaaac ttccaatggt tggaactttt
     4681 tcctttgaga tttccagtga ttggaagttt tattcccgcg cgccctgttt ggcccaggaa
     4741 tcccgcaggg tgaccgtgcg gttgaagacc ggccggccgg gcttggagtc gacgctgtcc
     4801 acgcagaaat agccgatgcg ctcgaactgg aatcgggtgc cgggttccgc cttcacgagg
     4861 gcgggttcaa tcaggcatgt cgtctcttcc agcgaattcg gattcagatg atccttccag
     4921 tctttgcctt caacagcatc catcggatct tccacgctga acaggcggtc gtagaggcgc
     4981 acttcggttt cgaccgcgtg ttttgcggat acccagtgga tggtgccttt gatcctgcgt
     5041 ccgtccggcg cgctgccgcc gcgcgagtcg ggatcccatg tgcagtgcac ttcggtgact
     5101 tcaccggcgg cgtttttgac gacatgggtg cattttacga tgcaggcata acgcaggcgc
     5161 acttcctgtc cgggggagag gcggaaatat ttcttcggcg ggttctccat gaagtcgccg
     5221 cgttcaatga acagttccct gcagaaggca accttgcgcg ttcctgccgc gggatcctcg
     5281 ggattgttca ccgcgtcgaa ttcctccacc ttgccctcgg gatagttgtc aatgacgagc
     5341 ttcagagggt tcatgacggc catgcgccgt aatgcagact tgttcaagtc gtcgcgcagg
     5401 cagtgttcca gcagagccac atcggtaagg ctttctgttt tggtgacacc cacgcgctcg
     5461 cagaaatcgc gaatggactc aggggtgtat ccgcgccgcc tgaggccgga gatggtaggc
     5521 atgcgcggat cattccagcc tttcacaagg ccttcttcga caagctccag cagcttgcgc
     5581 ttgctcatga cggtgtaggt gagattgagc cgcgcgaatt cgatctgccg cgaacggtag
     5641 atgtccagtt cttccaggat ccagtcgtac agcgggcggt gaacctcgaa ctccagcgtg
     5701 cagagcgagt gggtgatctt ttccagcgaa tcttcgatgc agtgcgcgaa gtcgtacatc
     5761 ggatagatgc accacttgtc gccggtgcgg tgatgatggg ccttcttgat gcggtagatg
     5821 gccgggtcgc gcatgtgcag gttcggagaa ttcatgtcta ttttggcccg gagcacatag
     5881 gctccgtcct cgaactcgcc ggccttcatg cgttcgaaca gatcgagact ttcctcgatc
     5941 ggccggttgc gccaggggct ttccttgccg gggcgcgtgg gcacaccgcg gtattccttg
     6001 aactcttcag ccgaaagcgt gcagacatag gccttgcctt ttttaatgag ctgcacggcg
     6061 aattcgtaca tctgatcaaa atagtcggag gcgtaataaa gatgtgtccc ccagtcaaat
     6121 ccgagccagc gcacatcctc catgatggat tcgacatatt cgacttcttc ggcggtgggg
     6181 ttggtgtcgt caaaacgcag gtggcaggtg ccgttgtttt cgagggccat gccgaagtct
     6241 atgcagatgg ccttggcgtg gccaatgtgc agatagccgt tgggctccgg cgggaaccgg
     6301 gttacaatgt gcgtgtgctt gccgcttttc aagtcatcgg cgatgatttg ccggatgaaa
     6361 tgcaacgggg ccgctgtttc gttttcactc ataaaaatac ctgcaatatc tggtgttatg
     6421 aacgcagtac ttttattcct tttgcattcc caacgcaata gaggattgtt ctttcatccg
     6481 gttctgctcc atagtatctg ttgtttttcg aagaacggag aaacatgaga aagatcagaa
     6541 cacgctttgc gcccagtccg acggggcatg tgcatattgg caacattcgt gcggccatct
     6601 acaactggct gtttgcgcat cataacggcg ggcagttcct gctgcgcatc gaggatacgg
     6661 acaccgaacg ttcgacaccc gaggcggtcc agacggtgct ggacgccatg gactggctcg
     6721 gactgaccta tgacgaggaa cctctttacc agtccaccca gcgggcgaag caccttgagg
     6781 cggcggagat gctgctgtca aaggggctgg cctacaagga ggacaagggc ggaacgggca
     6841 agggcgagtg catcatattc cggatgccgg ggcgggacat gactttccac gacctggtca
     6901 agggagcact gacgaaaaaa gccggtgaca tgaaggatct ggtaatcgtt cgttcagacg
     6961 gttctccggt atttcatctc gccaatattg tggatgacat cacgatgaat gtttcgcacg
     7021 tcattcgcgg cgatgaccat gttgagaaca cctaccgcca tgtggccatt tacgaggcgc
     7081 tgggcgcgga ggtgccgcag tacgcccatc tgccgatgat tgtcaacgcg cagggcaagc
     7141 cctattccaa gcgcgatggc gcagcctatg tcggcgaatt ccgcgaccgg gaatacctgc
     7201 cggaagcatt gttcaactat ctggcgctgc tgggatggtc gcccggcgat gaccgtgaaa
     7261 tcatgtcccg cgaggaaatg gtcgagctgt ttgatctcgc gcgcgcgcaa tcgtcgccgg
     7321 cccagatgga tctgaaaaag ctggaatgga tgaatggggt ttacatgcgg agccagccga
     7381 gagaggagct ggaaaagcgc tgccgtgagg tgatgatgca gcgaggactg tgggattcag
     7441 ccaccgcacc ggattatttc tcggccgtgc ttcgggccat gggcgatcgg attcacttct
     7501 attccgacat tgccctgcat gcgggtttct ttttcaccga gaactacgag tacgatgaga
     7561 aagctgtggc caagcggctg atgaaagagg gcgcattgga tgagctgcgc gaaatgggga
     7621 agcggttcgc ggccagtgat aagttcgatg ccccgtcgct ggacaagata ctccatgatt
     7681 atgcggcgga attgggcgcg aatgcgggac agcttatcca tccggtgcgc atcgcggtat
     7741 ccggcatggc agtgggtccg ggtctttttg aaatgcttga agtgctgggc cgcgagcgcg
     7801 ttctggcccg cattgagcgg acactgcgga aattcggagg ggcctaggag ccgaccatgg
     7861 gggattcatc agcaggctgt ccgcgcttga cgctcgccac gcgtatcacg cttttccgca
     7921 ttctttgtgt gccggtctat atcctgctgc tgatctatta ccagatgggg ctgaagcagg
     7981 gagccgccaa cgaatatttc cggagcggcg cgctgctggt atttattctg ctggcggcca
     8041 gtgactttct cgacggctac attgcccgca cgcgcaatca ggtcacccgg cttggaaccc
     8101 tgatggatcc cgttgccgac aaacttctga tgatatcctc gctgattctt ctgacccggc
     8161 catcgcttcc tgccctgcag ccccagttcc cggttctgat tgccgtgctc ttcatcagcc
     8221 gggatgccct gcttgttgcg ggagcctttg tggtgcagca tttcagcgga gaagtgcagg
     8281 tgaaaccccg ctggagcggc aaggcctcca cggcggccat ggcatgctgc gttgtctaca
     8341 ttctgctggg ctggcctgcg cactggttcc agccgctgct ggccgttgcg ggtatgctta
     8401 ttatgctttc attggttcag tatttgctgg atggtatccg gcagctggag ggcccggcca
     8461 taaaccggcc ggagaaggaa catcatgcct gagaataaag gtcagcagag agtggtggcc
     8521 attgtgggcc gtcccaatgt cggcaagtcg gcgctcttca accgcctggt cggccgccgc
     8581 gtggccatcg ttcacgaaga aagcggggtt acacgcgacc gtctgatgag cgaggccgtc
     8641 tgggatgagg agcactttga gttgatcgac acaggcggag tcgggatgat ggatggatcc
     8701 aggaccggca gcaccatcga ggacggaaca cgccagcagg tggatctggc cattcaggat
     8761 gcagcggctg tcatcttcgt tgtggacatc accgcggggg ttctccctct ggaccgcgag
     8821 gtggcgcgcc tgctgcatgc ttcgggtcgt ccggcttttg tggctgctaa caaggcagat
     8881 catcagaatc tgcagaatca ggccgatgat ttcgcggaac tgggattccc ggtgtatccg
     8941 gtgtcagcgc ttcacaaccg cggcgtcgat gatctgatgt ccggcgtgct cccggctctt
     9001 cctgctccgg ttccccgcac ggccattcag ccgctgaagg tggccgtggt gggccggccg
     9061 aatgccggaa agtcatccta tatcaaccgg cttctgcgca gcaatcgcgt gattgtgtct
     9121 gaagttccgg gcaccacccg cgacagcatt gaaatcccgt ttgccatcgg cagcggaaat
     9181 caggcccggc actatcggct tatcgacacc gcgggcattc gaaaaaccag caaggtgaac
     9241 acggcggtcg aaaaattcag tgtcatacgc gccgagaaca gcatcaggga agcggacgtg
     9301 gtggtcattg tgatggatgc caccgaggga ccgaagaaac tggacaagcg gattgcgtca
     9361 ctggctatca gcgagaacaa gggctgcgtt gtgctggtca gcaaatggga cctcgctgag
     9421 gggaccacca cccagcgcgc ctatggcaag gcgctgcgcg aggccgttcc gttcctgaac
     9481 ttcgcgccgg tggtcttcgc gtcggcaaaa acaggctaca acatccggaa caccattgag
     9541 gtgattgatc atgtcgcggc ccagctggac ctgaagctga ccacggggct tttgaaccgc
     9601 gtcatccagg attccgtggc gaaggttgct ccgcaggtgg tggggacgcg ccggctcaag
     9661 atattctatg ccactcaggt ggggacccgg ccgatccgta ttcgcttttt cgtgaactct
     9721 ccgcggcgca tcaaggaaaa ctacaagacc tatctgctcg cggcgctgcg caaggcgttt
     9781 ggtctcgaag gcgcaccggt tattcttcag ttccgcgccc ggcagcagaa aacggaacag
     9841 tgaattttca gttccgtttc cggtttgccc ggacggtgtc cataatccct gcgatgtaga
     9901 tcagcagggt tgtcagaaac gcaatcatca tgcgcatcag cggcagcgga ggcatctccc
     9961 gcgcctcgaa ctcaaatgca aaccggtaat attccctgag gatgaccgtc gcatacgcga
    10021 ggaagaacac cagagacagg gtcgcggcga tgccgaaaac ggcgccggct gcccatcgtt
    10081 tctggatcag ctgcccggcc cccggataaa cgagcgtcga aagcagcacc gcgagccggt
    10141 tcgcgttcaa gaaatcattt ctcctgatct gaaacagatg tgcacgggtc aggggagcgt
    10201 gtacccgatg cggtaataag cgctggtcga gaccatgttg gtgatgacca tttcaaggtc
    10261 tgctccggtg ccggtcatgg cgctgcccgc cacgatctgc cagttctgag tgggtaccag
    10321 aatcggagtt tcataaaccg catactccgc gccaaaaatg gtgctgccga ttgtcagcgc
    10381 gaatctcccg ccctcctgag cctctatggc atcgagaatc ggcgtgccgg tgcgtgtgat
    10441 gatcacggaa tcggtattgg tctggatgcc gcctgcggtg gggctggtga tcgcgaggtt
    10501 gttgaaatac atctggcgct catcgctgct gtccgtggcg ttgaagtaga acttgatgcg
    10561 gctgggtgcg ccggtggaaa cactgagggt ctggtcatag gattccagcc cgttgcgtcc
    10621 cgttccgcgg acacggatac tgccgtcggc gacgtattcg aagctcaagg gcatggcttg
    10681 agttccgtat tccgaaaaca gggtttctcc gttgatcgtg atcgtctgag tgttgcccat
    10741 attgatgttg atcagttctg tatcgccggc cagcaggttg aatccgcggt tggagccttc
    10801 gttgttgctg tcccagttca ggccgaagtc gaagcggaaa gtgctgccga tgccaagcgg
    10861 gctgctgaag tttctgatgg cttcggcatt ggcgccggaa ccgaacggat tggcatagaa
    10921 gccgaaggcc gtgtccccaa ggccgctgat gccggtattg gcggggtcgg cgatgaacgc
    10981 gccggcccat gaggtttcgc cgtcctgggt gtggttgaag gaccatccga ggaagccggt
    11041 gccgccgttg ttgccggagt tccagccgtc gctgtaggcg gcgtcggatg cggaatcaac
    11101 ggcgcttgtg acaactccgg gaggagcgga gtttgtgccc cgaacaatga tctcgttgcc
    11161 gccggaggcc agcgcaacat ttgtgaggga ccaggtggcg gcggccggaa gggtgcccga
    11221 ggctccggtc agcgtgttgc tccaggaaag aactccttcc atgccgtcgg cgattccgga
    11281 gaggtcatag gtttccgtgc tgttgggcac ggtgatgttc gagggcggat tggtgatggc
    11341 caggccggtc ggaagatcct cgcacccctc gacggcaaag tgccagtcgg ccccgccgtt
    11401 attgtcccat gtggcgccgc cgtcgttgaa caccatgttg atgttcgtgc tgccggccgg
    11461 cggagtgtac acataagtcc agtaggcccc ggatttggtc atgctcggat tgggcagtat
    11521 gacatcctgc catccgttgc ggccgatgtg gattctgacc ggatcggctc ccagcagcac
    11581 gcggcccgcc ggattgtagg tgatggtgat cggcccgcaa ccgaccgggt tggtcggact
    11641 gatcgaaacg ggcgggagag gggcttcttc ttcgccgcag ttggaaacgg cgacactcca
    11701 gtcggcgccg ctgttgttgt cccaaacccc cgctccgttg ttgaacacca cattgatttc
    11761 ggtggtgttg gtctccatga tatacgtgta ttcccagtag tacccgttct ggctcatgga
    11821 gggatcaggg gagatgacgt cctgccaccc gttgcggcca atgtgaattt tgacggcgga
    11881 agcctcctcc agcgggccct cgttcggata gtagcggatg gtcaccggat cgcagccgtc
    11941 gggggccgcc ggatcgaaaa caacggagga gggcccgacg ggcgcgtcgc agtcgcgaat
    12001 gggaaccttc cagttcgcgc cgttgttgtt ttcccagttg gtcccgtcat tgaacgcgac
    12061 ttccagctgc ggagcgttgt cgggaatgtc gttggtcgta aaggtgtgcg aaaaggtgtt
    12121 ggaactggtc cggcccatcg cggcgctcgc ccagtcattg gtgtttgtgg agaagtggta
    12181 gaacacattc accgttgccg ctgtcgccag cggcgatgtc gccgcgttga aattcaccgt
    12241 gatggacgcg cagtcggacg gcgcagcggg atcaaaagcc gcggaagggt acacgctgct
    12301 ctgtccgccg tcgtgctcta caaagacgtg ctgaatctct gatttgtgga cattgcccaa
    12361 actgtcatag gcctccacat agtagtcgag cagtttcccg cggaagccgg gaacattgcc
    12421 gtcgttgatt tcgacaaaat agtagtcggc gatgacggga ttggaccaga aggcggggtc
    12481 gaacacaaag tagtcaatct ggccgttggc ggcggcggtg ttcagggcgc tgcgcgtttt
    12541 cggcagttcg cgtttggtca tggccacggt catccatccg ccgacgtctg cgccgcccgc
    12601 gaaggtctcg ttgtgggtgg tggacagcga gttggtcccg tcgttgtcca gacgaacccg
    12661 gacgctgaca ttggtaatgc cgttgacatc ataggcatgg gtccagatgt agaattcaga
    12721 cggcattttc ttcaggtagc gggtgtcgcc tccggggata tagttgaacc agccgaaggt
    12781 gtaggctccg gggttgtagg ggaagcgctg cggtttgagc accgtgggcc cggtctggtc
    12841 cagatgcatg cgcgtggaca tataggactg cagcttgtca atggcgcgct tggtggcgag
    12901 cgcgggcttg ttctcgtcgt cgttgccgag ccctccgtag tagttgaatc cggaatccag
    12961 cccggtgaga tagatgtgcc aggccagctc gacatcattg ggactggtcc aggatccgtt
    13021 ccagtcgtag ggggcctgaa tcttccacgc ctgcacatcg ccgccctgat cgcgcatgat
    13081 ctgttcggcg gtgatgcacc agtttgcgcc ggccatgagc ggggcgtagc tgaagaattt
    13141 cagcgcgaag ccgggagtga ccagatcggc ctgagtgctg tcgtagcggt tggtggcatt
    13201 gatggcggtg ttgtacagcg ggggttcgat ccacttcatg aagttcgggg agccgtaatc
    13261 catttcaggg aatatccacg cgccgtcctc gatgtgcgca acgggggcat tagcctttgc
    13321 ggcatccaca aaaccctggg gtgtgttctt gttgtagccg gcgctggcgg aatcattgaa
    13381 gaactggggt gtggcctcca tccaggagct gcttccgccg ccccaggcat tatcgccgtc
    13441 ggaggacggc atgacgatca ccggccggtt cgggtcagtg gcatagggtg atatgttgtc
    13501 gttgatcttt ccgatgccct cattggcgta accgaagcgg tagctcagca cgtcgtcgga
    13561 cgggacggca tacatttcct tggtcgcgcc ggtgtcggga ttcacatatt gaaccttgtg
    13621 aagctggtag gcgtagggcg ccacgttcca ggccgcgttg cccggattcg gttcgctgta
    13681 ccaccagccc gatgtgggcg aaggaccgag cagatcggct ttgttgggcg ggcttgaata
    13741 gatgttgtaa gtgccggtcg gattggcgta gttgaaataa gtgggacagg tgcggctgat
    13801 atgatggctt gggacgatcg tccactcata gccctcgtcc acgaggacat caacgagatg
    13861 gcgggaatag gccatttcgg tcgggaagaa acccttggag tggtcgctca ggtcgctgtt
    13921 cccgccccag gccttccacc aggcctgctt gaaaatctgg agctctttcc ggaacacttc
    13981 cttggggagc agaggagcaa ggctgtggtg gtaagtgaag ccgacgagat caaggcggcc
    14041 ccagccgcgg gcttccgtgt tgccgctgtt ccagtcgccg ccgtagccca gatgaccgcc
    14101 gccgcccagc tgccgcacat tgtcaatgag cgatcccgaa taactcagcg caaagccgcc
    14161 gcccgagaaa gtggacagcg aattcttcgg cccgtactga taggcgttgc ggcggtcgtc
    14221 gaggccgaag atgttgccca gatcgttttc gggatggtag gccggactga tcccgccgta
    14281 gttctggccg ggtttgagga cgatggagtc ccatgcgtac tggacgcggg agttctggtc
    14341 gccgttgctg ttccattccg gccagtaagt gggctggtgg ttgtgccaga aacgggagac
    14401 atgca
//
LOCUS       DAOQWR010000056        14319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4700_length_14319_cov_5.203169, whole genome shotgun sequence.
ACCESSION   DAOQWR010000056 DAOQWR010000000
VERSION     DAOQWR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14319
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_14319_cov_5.203169"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /gene="uvrA"
                     /locus_tag="PKK36_06065"
     CDS             complement(<1..2117)
                     /gene="uvrA"
                     /locus_tag="PKK36_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR94160.1"
                     /translation="MSTKWITVAGAREHNLKNITVKIPRNALTVITGLSGSGKSSLAF
                     DTIYAEGQRKYVESLSAYARHFLEQLQKPQVDYIEGLSPAISIEQRTAGSNPRSIVAT
                     TTEIFDYMRLLYASIGHPHCPQCGKPITQQSAESIVDQILAWPERTRIVVAAPLVRGR
                     KGRHEDVLAGIRRRGFVRARVDGRVYELDDVPSLAKTRAHTIEVVVDRLVVGTDIRSR
                     LTDSIELALKEGEGTVIVLRPDDEKVSETVFSEKNACADCGISFEALLPRSFSFNSPY
                     GACPTCHGLGSMMVFDEDLIVPDLDKSLGSGAIRPWRGSGRRATMYYNRLLKALAAAH
                     GVDMDAPYRDLPKDFRKILMYGTGEREVTMKFWFRGATRTFRKAFEGVIPNLERRYRD
                     TKSEYIRKHLGAFMGKRHCTACDGARLRPEVLACTISGRSITDVCRLSVKDALKLFTT
                     LELTEQEARIASELIKEIRERLGFMQNVGLDYLTLDRESGTLSGGEAQRIRLATQIGA
                     GLMGVLYVLDEPSIGLHARDNERLIQTMIKLRDVGNTVIVVEHDEQTIRSADYVVDLG
                     PQAGRHGGEVVFAGPVNRLLKDTRSLTARYLRREMDSKIPAKRTPPHKGCLEIVGARE
                     NNLKNIHVKIPLGLLVCITGVSGSGKSTLIDDILHRALFRHFFGSGDRPGAHDRLKGV
                     EALDKVIVIDQSPIGRTPRSNPAT"
     gene            complement(2662..6540)
                     /locus_tag="PKK36_06070"
     CDS             complement(2662..6540)
                     /locus_tag="PKK36_06070"
                     /inference="COORDINATES: protein motif:HMM:NF023351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HNR94161.1"
                     /translation="MRKPCFAALLSTMVVLTCTQVYAQKPKTETRPEAPDMSWTMGMQ
                     AGEGSAEIFGDAIVPVYFTKSGAFLVNVRGSGSDNEEEEANAGLVYRHLFPEKDLIVG
                     GNIFYDSRWTAHDNRFDQMGVGLELLSRWIDARANLYLPENKEVLVDSFTDQTVEQRT
                     SGGWRDPYAESYHIAQDYVRRTETITTTYLFERFEQAREGWDAEVGVKLPVPDSLGEY
                     RLFAGYYNWDAQYTREDEISGFKARAEARILPALFLDASWYEDKELNGSDWFIGARLS
                     LPFDLGLLAEGKNPFAGAFSRPKPAPFAARLFEMVIRDLKIQTEDSGYVENEKARAVD
                     VKRSSSRSTVVLMDNLTFVDDSNMTGIENGTAEYPFNTIQEGVNAANSPVYVFSGMYN
                     ENVVMRANVDLYGEGYGIPGMGGRVFGGQVYPVANGRSRGPTFTMVSNSSITGMRILN
                     TDVPGAPPILDFVDGNPYDISRVAVFGHDANNILIRQNQIGRSQHGIFLGVDSTPNYV
                     ARLQGNRISGAVDDGVRVEAVGGPGSSFTLLSGGNSYSGNGSDGIDVDVEDFDNVFLS
                     FAGDRASGNQNDGIDMDSCEDNFDMLVQIRNSTANNNAGEGFELGGVDVDGIFQGYFD
                     GLKAEGNAGEGLDLEVYAPGPILAVVADSSFNNNLGNGMQMILNSDYLSVGLVGPDAA
                     LFESVNMLLGGFLPPQMRSFSGPGTVTANGNAGDGMVLISDADSVALTGIFDARASGN
                     AQNGIFSVANSDFGLAASLALPSSDLLDVMDFGLGALSSLLGGPAMPNLPNAADQGPI
                     RVNGNGGAGFVSIVDGPVALSAQWDIQANGNNGSGIESIVLGDNVGISLQGGLEASYN
                     NGVGIESVVIGDNAAIGVVLDTIANHNAGPGIAMLRQSNNGIAVGLVSSVRSLLDGAE
                     TLLNAAGLGPIRLPNYTQGSGTVASGNGAGGILLQVDGEDAAIAGLMDVVANNNLGGP
                     GIGIRLDSNNGMALGGLVDVAASGNAQNGIDLRANADSVAIGALFDVTANRNGDDGTR
                     VVLTSLNDDAVAIAAGITANNNGNNGANFNLEADNDVYGLYTDMSLLGNLNNGLRVNA
                     TAGDDVYGFYGEGMVAIFSAATLGFPPSLLALVPDGGITASGNGTDGVRVTGDAIGGD
                     YATFMDSVVAANNGSDGIRINPTAGDDAEVGIMNATAIGNGIHGIRVNLTAANDISTI
                     ISGSISANNLNHGVSVVAAAGGLTQIDLGGGAFGSAGMNSIYGNAIDLRYNGPGTAVA
                     QFNWWGTATPVAGQFSGSIDWSNPLAADPN"
     gene            complement(6699..>7003)
                     /locus_tag="PKK36_06075"
     CDS             complement(6699..>7003)
                     /locus_tag="PKK36_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNR94162.1"
                     /translation="RLRLEKMVKVLREIGFDAKMPGGTFYLYVSAPKAAGDLVFKNAE
                     DASQYLIREHSISTVPWDNVGAFLRFSATFESAGDADDDRVITELKKRLKSANLKF"
     assembly_gap    7004..7103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7104..8074)
                     /locus_tag="PKK36_06080"
     CDS             complement(<7104..8074)
                     /locus_tag="PKK36_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR94163.1"
                     /translation="MNLQKLFAERIGGEKFGQSNEIYKFEKIKRAKAAARAARPDVEM
                     LDFGVGEPDRMAPQAIRDALKREADVPANRGYADNGVLVFKQAAAQYMKEFFGVTDLD
                     PVTEINHSIGSKPALAMLPLAFINPGDIAFQTVPGYPVMATHVKYLGGKVVDLPLRKE
                     NGFYPDLDAIDPAVADKAKLIYVNYPNNPTGAAPTPEFFDKLIAFAQKHNILVIQDAA
                     YATLIYGQRLSIFNRPGGKEVALELHSMSKSYNMTGWRLGFVAGSARAVQAFAEVKDN
                     IDSGQFIAIQKAACAGIANKQLSEEIREHYRLRLEKMVKVLREIGFDA"
     assembly_gap    8162..8261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8303..8761)
                     /locus_tag="PKK36_06085"
     CDS             complement(8303..8761)
                     /locus_tag="PKK36_06085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885594.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNR94164.1"
                     /translation="MDELLMTAWGKFGGAAALCLAAVGSALGAGAAGPAAIGAWKKCY
                     AQGKAAPFTLVTFVGAPLSQTIYGMILMNKIMQIAASAPQSWPLLVSAGVFGGLAMGA
                     SAWYQGKAGAAGSDALGETGQGFGNYLMVLGVIETVALFVMVFLLMAVGK"
     gene            complement(8808..10562)
                     /locus_tag="PKK36_06090"
     CDS             complement(8808..10562)
                     /locus_tag="PKK36_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94165.1"
                     /translation="MIVPMKKVTVLCLDHARDETLEALRALGTVHLSHLQLPEGEEVE
                     KARARLAYVERALEILPPHPQQAPSGKTAEQVVTALWALIHEQKDVRENLAHLKHERI
                     RVEPYGSFDPESIRKLAGKGIHVKLYQAPPGADMQAPPDCALQVLSSTKEGVHFAVIG
                     RGDFSLNAVEFVPPSRSLSEIEAETAAAEKRLAGIDEEISAFGGDHPAVARMVQDARD
                     HVRYAEARAGMGAEKAIAYLQGYCPASSVSMIEAASAEKGWGLVISEPSADDPVPTKI
                     ENPGWIKPIKAIFDMTGILPGYHEVDISVPFLIFLSIFFAMLVGDAGYGLLFVWLTRF
                     ARKKMPKAPAYPFTLLYIMSAATIVWGLLTRTVFGMPKHMLWEARIPWLEDDTNVMQL
                     CFIIGAVHLSLAHLWNAWLLRRTPQALAHIGWTCTTWGMFFTAGMFVLGKTFPSWAAP
                     LMIAGVLLIVLFKTPVKQLKSEWFGHAMLPLDIIANFTDVVSYLRLFAVGTASFAVAN
                     AFNQMLTPMFGGLLSGLVAAVLLFLGHALNIALCGLGILVHGVRLNTLEFSSHMGVQW
                     SGRPYRPFCKQCEKDLNK"
     gene            complement(10559..11161)
                     /locus_tag="PKK36_06095"
     CDS             complement(10559..11161)
                     /locus_tag="PKK36_06095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNR94166.1"
                     /translation="MAKIKHTKNELKAQRDALRRFERYLPTLLLKKQQLQTEMRTLDQ
                     KLSAKTGEVEALRSDMASWVRLFSEPFDFQSCLTVTDIRISEGNIAGVNIPVLEGVDF
                     SRQVPDLFESPLWVDDALAAVEQLTRLSVEQAVMERQYQLLKEELRTTTQRVNLFEKV
                     KIPECREHIRVIRIFLGDQQTAAVARAKIAKGKAPKEVAA"
     gene            complement(11168..12484)
                     /locus_tag="PKK36_06100"
     CDS             complement(11168..12484)
                     /locus_tag="PKK36_06100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNR94167.1"
                     /translation="MHKVYHRIEEIAGNVIMVKAAGVTYRELARVTWKNGDSLAQVIR
                     MNGERVSLQVFAGGRGIPTDARVRFLGHSMQTAFSEALLGRVFTGSGEPRDRGPSISE
                     NMIPIGGPSVNPAKRIIPRNMVRTGIPMIDVFNTLVESQKLPIFSVAGEPYNPLLARI
                     ALQAEVDVIILGGMGLKHDEYLFFRDTLEEHGALSRSVLFIHTAADPTVECLMVPDIC
                     LAVAEQYALQNKRVLVLLTDMTNFADSMKEIAITMEQIPSNRGYPGDLYSQLAARYEK
                     AVDFDSAGSITILAATTMPGDDVTHPVPDNTGYITEGQFYLRNNRIEPFGSLSRLKQL
                     VNSKTRKDHRTIMNTMIQLYAGFKETLEKQAMGFRMSAWDEKLLKYGERFENELMSLS
                     VNIPLEAALDLGWQILADCFEPAETGIPSALTEQFWPKDKKASAKE"
     gene            complement(12477..14210)
                     /locus_tag="PKK36_06105"
     CDS             complement(12477..14210)
                     /locus_tag="PKK36_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNR94168.1"
                     /translation="MSENIGKIVGVNGNLLTVEFSSPVIQNEVGYAHVKGEKLKSEVI
                     RIRGSRAELQVFEDTTGLMVGDDVQFTGELLSVELGPGLLSQVYDGLQNPLPALAAEA
                     GFFLKRGMYLQALDRAKKWAFTPAVKKGATVSPADALGTVPEGIFEHAIMVPFHLKGK
                     LTVEEISAAGEYDVEKVIARLKDAEGQLVEVKMYQRWPVKIPIRQYAERLRPTEPLVT
                     KVRIVDTFFPVARGGTYCIPGPFGAGKTVLQQITSRYADVDVVIIAACGERAGEVVET
                     LREFPELTDPRTGKSLMERTLIICNTSSMPVAAREASVYTAVTIAEYYRQMGLHVLLL
                     ADSTSRWAQALREVSGRLEEIPGEEAFPAYLESVIASFYERGGIVRLNSGKNGSVTIG
                     GTVSPAGGNFEEPVTQSTLKVVGAFHGLSRERSDARRYPAIDPLDSWSKYFSVVDEKD
                     LEFARRVLREGSEVNQMMKVVGEEGTSIDDFQLYLKSEYLDAVYLQQDAYHEIDGATS
                     AERQKYVFGVIAKIMRTKMSFADKEAARRFFQNLTQVTKDWNRQDMESEEFRALEERI
                     EKMIAEVKEYA"
BASE COUNT         2883 a         4416 c         3904 g         2916 t
ORIGIN      
        1 gttgccggat tgctccgggg ggtgcggcca atgggagact ggtcaatcac gatcaccttg
       61 tccagggcct ccactccttt cagccggtcg tgtgcgccgg gcctgtcgcc cgacccaaag
      121 aaatgacgga acagggcccg atgaaggatg tcatcaatca gcgtgctctt cccgctcccg
      181 gacacaccgg tgatgcacac cagcagtccg aggggaatct tcacatggat attcttcaga
      241 ttgttctccc gcgcgccgac gatctccaga cagcccttgt gcggcggagt gcgcttcgcg
      301 ggtattttcg aatccatctc gcgccgaagg tagcgcgcgg taagagaacg ggtatccttc
      361 agaagcctgt tcacagggcc ggcgaacacc acctctccgc catgcctgcc ggcctgcggg
      421 cccagatcta ccacatagtc ggccgagcgg atcgtctgct cgtcgtgctc gaccacaatg
      481 accgtgttgc ccacatcacg cagcttgatc atcgtctgga tcaggcgctc gttatcccgc
      541 gcatgcagtc cgatgcttgg ttcatccagc acatagagaa cacccatcag ccccgcgccg
      601 atctgcgtgg ccagccgtat gcgctgggcc tcgccgccgg aaagcgttcc gctctcgcgg
      661 tcaagcgtaa ggtaatccag tcccacattt tgcatgaagc ccagcctttc acgaatctcc
      721 ttgataagct ccgaggcgat tctggcttcc tgttctgtca gttcgagcgt cgtaaacagc
      781 ttgagcgcat ctttgaccga caaacggcag acatcggtga ttgagcgtcc cgaaattgtg
      841 caggccagca cctcaggacg aagccgcgcg ccgtcgcagg ctgtacaatg acgcttcccc
      901 atgaacgctc ccagatgctt gcggatatat tcgctcttcg tatcgcgata ccggcgctcc
      961 agattcggga tcactccttc aaacgccttg cggaaagtcc gggtcgcgcc gcgaaaccag
     1021 aacttcatcg tcacttcccg ctctccggtc ccatacatga ggatttttct gaaatccttc
     1081 ggcagatctc tgtagggcgc atccatgtcc acaccatgcg cggccgcaag cgctttcagc
     1141 agccgattat agtacatcgt cgcgcggcgg ccgcttcccc gccacgggcg tatcgcgccg
     1201 ctgcccagcg acttgtccag atcgggaacg attaaatcct cgtcaaacac catcatgctc
     1261 cccagcccat ggcaggtcgg acaggccccg taggggctgt tgaacgagaa cgagcgcggg
     1321 agcagcgcct caaaactgat gccgcagtcc gcacaggcat tcttctccga aaaaaccgtt
     1381 tccgagactt tctcatcatc cggcctcagc acaataacgg tcccctcgcc ctccttcagt
     1441 gcgagttcga tcgaatccgt cagacgcgat ctgatatcag tgccgacgac caggcggtca
     1501 acaaccacct cgatcgtatg ggcccgggtt ttcgcaagcg aaggcacgtc gtccagttcg
     1561 taaacccggc catccacccg ggcccggaca aaaccccggc ggcggatgcc ggccagcaca
     1621 tcctcgtgcc ggcccttgcg gccgcgcacc agaggcgcgg cgaccacaat ccttgttctc
     1681 tccggccatg cgagaatctg gtcaacaatg gactctgcgc tctgctgcgt gatcggcttc
     1741 ccgcactgcg ggcagtgcgg atggccgata ctggcgtaca gcaggcgcat atagtcgaat
     1801 atctccgtcg tagtggccac aatggaccgg gggttggagc cggcggtgcg ctgctcgatc
     1861 gaaattgcgg gggagagacc ctcaatgtaa tccacctggg gcttctgcag ctgctcgaga
     1921 aaatgacggg cataggccga caggctctcc acatacttgc gctgaccctc cgcataaatc
     1981 gtgtcgaacg caagagagga cttccccgaa ccgctgagac cggtgatcac agtgagtgca
     2041 ttgcgcggta ttttcaccgt gatgttcttc aaattgtgtt cgcgcgcgcc cgcgaccgtg
     2101 atccattttg tactcataga aaaacctgat aactgtccgg cagaaactag cattccgccc
     2161 ggccaaacgc cagcctcttg aacaagttca aacggcgggc tgtatgggaa ggcattggga
     2221 tgcggccggc tggaaatggc aagagaaaga aatggggact gaggtgtact atgccggctc
     2281 tcgtgaaagt gccccataag gaagaacacc tgaaatacgg cggcaaaccg gaacattgat
     2341 gttcatgttc gaggctccat cctgttttcg cgcctttcgc gcttttcgca gttccattta
     2401 tttctttgga gatgttcgct gcggcgaaac cgtttttttg aaaggttcga acaaagttgc
     2461 ccaggcggaa agtatcaggc ttcagtctcg tcggccccat gagacgacga tgacatttca
     2521 gccccgatct tcattctgcc ggctgatcaa ggatttggaa aaagaacagg gtccacatct
     2581 tttcactgat tactccaaat ctgaccctga ccatccgctt atccaacccg ccagaggcca
     2641 gatgagcctt gatctcacgc atcagttggg atcagcggcc agaggatttg accaatcaat
     2701 tgatccactg aactgcccgg ccacgggggt ggcggttccc caccagttaa actgggcaac
     2761 tgccgtcccg ggaccattgt accgaagatc tatggcattg ccgtatatgg aattcattcc
     2821 ggccgaacca aaggctccac cacccagatc aatctgcgtc aggcccccgg cagccgcaac
     2881 gaccgatacg ccatgattaa gattattcgc actgatgctc ccggaaataa tggtggatat
     2941 atcattcgcg gccgtgagat tcacgcgaat gccgtgtatg ccgttgccga ttgcagtcgc
     3001 gttcattatg ccgacttcag catcatctcc ggcagtggga ttaattctta tgccgtcaga
     3061 accattattg gcggccacaa ccgaatccat aaaggtcgca taatcacctc cgatggcatc
     3121 tcctgtcaca cggactccat ctgtgccgtt cccgctggcg gtgattcccc catcggggac
     3181 aagggcaagc agtgacgggg ggaagcccag cgttgccgcc gaaaagattg cgaccatgcc
     3241 ttctccatag aatccgtaca catcatctcc cgcggttgcg ttcacccgca gaccgttgtt
     3301 cagattccca agaagagaca tatcggtgta caagccataa acatcattgt cggcttcaag
     3361 gttgaagttg gcgccgttat tgccattgtt gttggccgtg attcctgccg ctattgcaac
     3421 ggcatcatca ttcaggctgg tcaggacaac acgtgttccg tcatccccat tacggtttgc
     3481 cgttacatca aaaagagctc caatggcaac cgaatccgca ttcgcacgca gatctattcc
     3541 attctgggcg ttgccgctgg ccgcaacatc caccagtccg ccaagcgcca ttccgttgtt
     3601 gctgtcaagt ctgatgccaa taccggggcc gccaagattg ttgtttgcaa caacatccat
     3661 gaggccggca attgccgcat cctcgccatc gacttgcaga agtatgccac cggcgccgtt
     3721 tccgctagcc actgttccgg atccctgcgt atagttcgga agcctgatcg ggcccaggcc
     3781 cgcggcattc aggagcgtct cggccccgtc cagcaggctg cgcacagacg acaccaggcc
     3841 aacagcaatc ccattgttgc tttgccgaag cattgcaata cccgggccag cgttatggtt
     3901 cgcgatggtg tcgaggacaa cgccaatggc cgcattatcc ccgataacaa ccgactctat
     3961 gccaacaccg ttgttatagc ttgcctcgag gccgccctgc agggaaatgc caacgttatc
     4021 acctaaaacg atggactcta tgccggagcc gttgttcccg tttgcctgaa tgtcccactg
     4081 ggcggacaga gcaacaggac cgtcaacaat ggatacaaat cccgccccgc cgttgccgtt
     4141 cacgcggatc ggtccctgat ctgctgcatt cggaagattc ggcatcgccg ggccgcccag
     4201 cagggaggac agggcgccaa gaccaaagtc catcacatcc agcagatccg aactcggcag
     4261 agccagggag gccgccagcc cgaagtcgct gttggcgacc gagaaaattc cgttctgggc
     4321 attgccgctg gcgcgcgcat cgaagatccc tgtcagcgca acagaatccg catcagatat
     4381 gagcaccatg ccgtccccgg cgttcccgtt ggccgtcacg gttccgggtc cggaaaacga
     4441 cctcatctgg ggcgggagga aaccgcccag cagcatgttc accgactcaa acagcgccgc
     4501 atccgggccc accagcccca cggagaggta gtcgctgttc aggatcatct gcattccgtt
     4561 gccgaggttg ttgttgaagg aagaatccgc cactacggcc aggataggcc ctggggcata
     4621 gacttccaga tccaggcctt cgcccgcgtt gccctccgcc ttcagcccgt caaaatagcc
     4681 ctggaatatg ccgtccacat caacgccgcc caactcgaat ccttcaccgg cgttgttgtt
     4741 tgccgtcgaa ttcctgatct gcaccagcat gtcgaagttg tcctcgcagc tgtccatgtc
     4801 tatgccgtcg ttctggttgc cgctggcgcg gtcaccggca aagctgagaa agacgttgtc
     4861 aaaatcctcc acatccacat caatgccgtc ggacccgttc ccggaatagc tgttgccgcc
     4921 ggagagcagc gtgaagctgc tgcccggtcc gcctaccgct tcgacccgga ctccgtcatc
     4981 aaccgctccg ctgatgcggt tgccctgcag tcgcgccaca tagttggggg tgctgtcgac
     5041 gccgaggaat atgccgtgct ggctccggcc aatctggttc tggcggatca ggatgttgtt
     5101 cgcatcatgc ccgaacacgg ccacgcggct gatgtcatag ggattgccgt ctacaaaatc
     5161 aagaatgggc ggggcgcccg ggacatccgt gttgagaatg cgcatgccgg tgatggagga
     5221 attgctcacc atggtgaacg tcgggccgcg cgagcggccg ttggccacgg gataaacctg
     5281 gccgccgaac acacgccctc ccatgcccgg gatgccgtac ccctcgccgt acagatccac
     5341 attcgcccgc atgacgacat tctcgttgta cattccgctg aagacataaa ccgggctgtt
     5401 ggcggcattc acgccttcct gaatggtgtt gaaggggtac tcagcggttc cgttttcaat
     5461 cccggtcata ttgctgtcat ccacaaaggt gaggttgtcc atcagcacca ccgtgctgcg
     5521 cgaggacgac cgcttgacat ccacggcccg ggccttctcg ttttccacat accccgaatc
     5581 ctcggtctgt atcttcaggt cgcgaataac catctcaaaa agccgcgcgg caaacggggc
     5641 cggtttcggg cgggagaagg cgcccgcaaa cgggtttttc ccctccgcca gaagccccag
     5701 atcaaacggg agactcaggc gggctccaat aaaccagtcc gatccattga gttccttgtc
     5761 ctcataccac gatgcatcaa gaaacagtgc cggaagaatt cgtgcttccg cgcgcgcctt
     5821 gaagccgctg atctcatcct cacgggtgta ctgggcatcc cagttgtagt agccggcaaa
     5881 caggcggtac tcgcccagac tgtcgggaac aggaagcttc acgcccactt ccgcatccca
     5941 cccctcgcgc gcctgctcga accgctcaaa aagataggtc gtggtgatgg tttccgtccg
     6001 ccggacatag tcctgcgcta tgtgataaga ctccgcatag ggatcgcgcc acccccccga
     6061 ggtccgctgt tccacggtct ggtccgtgaa agagtccacc agcacctcct tgttctccgg
     6121 cagataaagg ttcgcccgcg catcaatcca gcggctgagc agttccagcc caacccccat
     6181 ctggtcgaac cggttgtcat gcgccgtcca gcgcgagtca taaaagatgt tgccgccgac
     6241 aatcaggtct ttctctggaa aaaggtggcg atacaccagt ccggcattcg cctcctcctc
     6301 ctcgttgtcg cttcccgaac cgcgcacgtt cacaaggaat gcgccggatt tcgtgaagta
     6361 gacggggaca atcgcatcgc caaatatctc ggccgatccc tcgcctgcct gcatgcccat
     6421 ggtccaggac atatccggcg cttcggggcg ggtctcggtt ttcggtttct gcgcataaac
     6481 ctgcgtgcag gtcagaacaa ccatcgtgga caacagagcg gcaaaacaag gtttcctcat
     6541 aaccatacct cctcttttgc ccggcattct atttgctgca cgtataaatt gtcgataaaa
     6601 aaatgcagaa tcgggaaatg aacgggaaac ccatatgaca aggtcgctga cctgaaaacc
     6661 cgaacaggcc gtccgtataa aaacgcgcga tgcctgcgtc aaaatttcag atttgctgat
     6721 ttcaggcgct ttttcagctc agtgatcacg cggtcatcat cggcatcgcc ggcggattcg
     6781 aaggttgccg aaaaacggag gaatgcgccg acgttgtccc acggcacggt ggatatggag
     6841 tgctcgcgga tcaggtactg gctggcatcc tcggcatttt tgaaaaccag gtcgccggcc
     6901 gctttcgggg cgctcacgta cagataaaaa gtgccgccgg gcatttttgc gtcgaaaccg
     6961 atttcgcgca gaactttcac cattttctca agccgcaggc ggtnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnngcgtcga aaccgatttc gcgcagaact ttcaccattt
     7141 tctcaagccg caggcggtag tgctcgcgga tttcctccga aagctgcttg ttggcgattc
     7201 cggcgcaggc cgccttctgg atcgcgatga actggccgga gtcaatgttg tccttcactt
     7261 cggcgaaggc ctgcacggca cgggcggagc ccgccacgaa accgagacgc cagccggtca
     7321 tgttgtaact cttgctcatc gaatgcagtt ccagcgccac ttccttcccg ccggggcggt
     7381 tgaagatgga cagccgctgg ccgtagatca gcgtcgcgta ggccgcatcc tgaatgacaa
     7441 gtatgttgtg cttctgcgcg aaggcgatca gcttgtcgaa gaactccggc gtcggcgcgg
     7501 cgccggtcgg attgttggga tagttcacat agatcagctt ggccttgtcc gcgacagccg
     7561 gatcaatggc gtccagatcg ggatagaatc cgttttcctt gcggagcggg agatccacaa
     7621 ctttaccgcc cagatatttc acgtgcgtgg ccatcaccgg atagccgggc acagtctgga
     7681 aggcgatgtc gccgggattg atgaacgcca gcggaagcat ggccagcgca ggcttggaac
     7741 cgatgctgtg attgatttcg gtgacgggat caagatcggt gacgccaaag aattccttca
     7801 tgtactgcgc ggcggcctgc ttgaagacca gcacgccgtt gtcggcatag ccgcggttgg
     7861 cggggacatc cgcttcgcgc ttgagggcat cgcgaatggc ctgcggagcc atgcggtcag
     7921 gctcgccgac gccgaagtcc agcatttcga catccgggcg cgcggcgcgg gccgcggcct
     7981 tggcgcgctt gatcttttca aacttgtaaa tctcgttgga ctggccgaat ttctccccgc
     8041 cgatccgttc cgcaaaaagt ttctgcaagt tcatttcaac tcctgaatgt tagccgcaaa
     8101 gaggcacaaa atgcacaaga cgcggaagat attcaatccc ttttcgactt cattgccgcc
     8161 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagcctaaa ttccttcagc
     8281 ccgagcatcc tgcgggaggc tcttatttcc ccacagccat cagcaggaag accatgacga
     8341 agagggcgac ggtttcgatg acaccgagga ccatcaggta gttgccgaag ccctggccgg
     8401 tttcgcccag cgcatcggat ccggccgcgc cggccttgcc ctgataccag gccgaggcgc
     8461 ccatcgccag cccgccgaac acgccggcgg acaccagcag cggccagctc tgcggcgctg
     8521 aggcggcgat ctgcatgatc ttgttcatca gaatcatgcc gtaaatcgtc tgcgagagcg
     8581 gcgcgccaac aaaggtcacc agcgtaaacg gagcggcctt gccctgcgcg tagcattttt
     8641 tccacgcgcc gatcgccgcc ggcccggcgg ctcccgcgcc cagtgcagat cccactgcgg
     8701 cgagacacaa tgccgcggcg ccgccaaatt ttccccaggc tgtcatcaat agttcgtcca
     8761 tgatacttcc ctttctcccg ttgctgcgcg ggattaaccc gctgatttca tttattcaaa
     8821 tccttttcac actgtttgca gaacggacgg tacggccggc ccgaccactg cacccccata
     8881 tgcgatgaaa attcaagcgt gttcaggcgc acgccatgca ccagaatgcc gagcccgcac
     8941 agcgcaatgt tgagcgcatg accgaggaac agcagaacag ccgccaccag accgctcagg
     9001 agtccgccga acatcggcgt cagcatctgg ttgaaggcgt tggccaccgc aaaggaggcg
     9061 gttccgacag cgaacaggcg caggtaggac accacatcgg tgaagttggc aatgatgtcc
     9121 agcgggagca tcgcatgacc gaaccattcg cttttcagct gcttcacagg ggtcttgaac
     9181 aatacgatca ggaggacgcc cgcgatcatg agaggcgcag cccacgacgg aaaagtttta
     9241 ccaagaacga acatgccggc cgtgaagaac attccccatg tcgtgcaggt ccagccgatg
     9301 tgggccagcg cctgcggcgt cctgcgcagc agccacgcat tccacagatg ggccagggag
     9361 agatgaaccg ctccaatgat aaagcaaagc tgcatcacat tcgtgtcgtc ctcaagccac
     9421 gggattcttg cctcccacag catgtgcttc ggcatcccga acaccgtccg ggtcagcagc
     9481 ccccagacaa tggttgccgc gctcatgatg tagagcagtg taaacggata ggccggggcc
     9541 ttcggcattt ttttccgcgc gaaacgcgtc agccagacaa acagcagtcc gtaaccggca
     9601 tcaccgacaa gcatcgcgaa gaaaatgctg aggaaaatca gaaacggcac gctgatatcc
     9661 acctcgtgat agccgggcag gatgcccgtc atgtcaaaga tggccttgat gggttttatc
     9721 catccggggt tctcaatctt ggtgggcacc ggatcatctg cggaaggctc cgaaatcacc
     9781 aggccccagc ccttttccgc ggacgccgct tcaatcatgg acacagatga ggccggacag
     9841 taaccctgca gataggcgat tgccttttca gcgcccatgc cggcgcgggc ttcggcatag
     9901 cgcacatgat cccgcgcgtc ctgcaccatc cgggccactg cgggatggtc accgccaaag
     9961 gccgaaattt cttcatctat gccggccagg cgtttttccg cggcggcggt ttccgcctca
    10021 atttcgctga gcgaccggga cggcgggaca aactccaccg cgttcaagct gaaatcgccg
    10081 cggccgataa cagcaaaatg aacaccctct ttcgtggagg acagcacctg caaggcgcag
    10141 tccggaggcg cctgcatgtc tgctcccggg ggcgcctggt acagcttcac atgaattcct
    10201 ttgccggcca gtttccggat cgattcagga tcgaatgaac catacggctc cacccgaatc
    10261 ctctcgtgct tcaaatgagc cagattctcc cggacatcct tctgctcgtg tatcagcgcc
    10321 cacaaagcgg tcacaacctg ttcggctgtt ttaccggacg gagcctgctg aggatgcggc
    10381 ggcaggattt cgagggcgcg ctcgacatac gccagccggg cgcgcgcctt ctcgacctct
    10441 tcgccctccg gaagctgaag atggctcaaa tgcactgtgc cgagggcgcg cagggcctcc
    10501 agtgtttcgt cccgggcatg gtcaaggcag agcaccgtga cttttttcat ggggacgatc
    10561 atgccgccac ctccttcggc gccttgccct tggcgatctt ggcgcgggcc acggcggcgg
    10621 tctgctgatc gccgaggaaa atgcggatca cgcggatatg ctcgcggcat tccgggatct
    10681 tgaccttttc gaaaagattc acgcgctggg tggtcgtgcg cagttcctcc ttcaggagct
    10741 ggtactgccg ttccataacc gcctgctcga cgctgaggcg ggtcagttgc tccaccgccg
    10801 cgagggcatc gtccacccac agcggcgatt caaagagatc cggcacctga cggctgaaat
    10861 ccacgccctc cagcaccggg atgttgacgc cggcgatgtt tccttcgctg atcctgatat
    10921 ctgtcacggt caggcaggac tggaaatcga atggctcact gaaaagccgg acccatgagg
    10981 ccatatcact gcgcagagct tcgacttccc ctgttttcgc cgaaagcttc tggtccagtg
    11041 tgcgcatttc ggtctgcagc tgctgtttct tgagcagcag ggtgggaaga taccgttcga
    11101 acctccgcag cgcgtcgcgc tgcgccttca gttcgttttt ggtatgttta atcttcgcca
    11161 ttcgcgctta ttccttcgcc gacgccttct tgtccttcgg ccagaactgc tcggtcagcg
    11221 ccgacggaat tcccgtttcg gccggctcaa agcagtcggc cagaatctgc cagccgaggt
    11281 ccagcgccgc ctccagcggg atattgaccg acagggacat cagttcattc tcaaaacgct
    11341 caccgtactt gagaagcttt tcgtcccatg cgctcatgcg aaagcccatg gcctgctttt
    11401 cgagggtctc cttgaaaccg gcataaagct ggatcatcgt gttcatgatc gtgcggtgat
    11461 ccttgcgcgt cttgctgttg accagctgtt tcaggcggct cagcgacccg aagggctcaa
    11521 tgcggttgtt gcgcaggtag aactggcctt ccgtgatgta tccggtgttg tccggaaccg
    11581 gatgcgtgac gtcgtcgccc ggcatggtgg tcgcggccag aatcgtgatg gagccggcgg
    11641 aatcaaagtc caccgccttt tcataacggg ccgcgagctg gctgtagagg tcgcccggat
    11701 agccccggtt ggagggaatc tgctccattg tgatggcaat ttccttcatc gagtcggcga
    11761 agttcgtcat gtcggtcagc agcaccagca cacgcttgtt ctgcagcgcg tactgctcgg
    11821 ccaccgccag acagatgtcc ggcaccatca ggcattccac cgtcggatcg gccgcggtat
    11881 gaataaacag gaccgagcgt gaaagcgccc catgctcctc cagcgtgtcg cggaagaaaa
    11941 gatattcgtc gtgcttgagg cccatgccgc ccagaataat cacatccacc tcagcctgca
    12001 gggcgatgcg cgccagcagc gggttgtagg gctcgcccgc aactgaaaat atcgggagtt
    12061 tctgcgactc aacgagcgtg ttgaacacgt caatcatggg gatccccgtg cgaaccatat
    12121 tgcggggaat aatgcgcttg gccggattca cggacggacc gccgatcggg atcatgttct
    12181 cggagatgga ggggccgcgg tcgcggggtt cgccgctgcc ggtgaaaaca cggcccagca
    12241 gcgcctcgga aaaggcggtc tgcatgctgt ggccgaggaa gcgcacgcgc gcgtcggtcg
    12301 ggatgccccg gccgccggca aacacctgca gcgaaacccg ctccccgttc atgcggatga
    12361 cctgagcaag agagtccccg ttcttccagg tcacgcgggc cagttcgcgg taggtcaccc
    12421 cggcagcctt caccatgatg acgttgccgg caatctcttc aattcggtgg tagactttat
    12481 gcatattcct tcacctctgc gatcatcttc tcgatgcgct cttccaatgc cctgaactct
    12541 tctgattcca tgtcctgcct gttccagtcc ttggtcacct gcgtcaggtt ctggaaaaac
    12601 ctgcgcgcgg cttccttgtc cgcgaaggac atcttcgtcc gcatgatctt agcgatgacg
    12661 ccaaagacat acttctgccg ttcggctgaa gttgcgccgt caatctcatg ataggcgtcc
    12721 tgctgaagat aaacggcgtc aagatattcg cttttcagat aaagctggaa gtcgtcgatg
    12781 gaggtgccct cctcgccgac caccttcatc atctggttga cctcgcttcc ctcgcgaagc
    12841 acgcgccggg cgaactcgag atccttttca tccaccacgc tgaaatattt gctccagctg
    12901 tcaagcgggt caatggcggg atagcggcgg gcatccgaac gttcgcggga gagtccgtga
    12961 aacgcgccaa ccaccttgag cgtgctctgc gtgacgggct cctcgaaatt ccctccggcg
    13021 ggactgacgg ttcctccaat ggtcacggac ccgtttttcc cgctgttcag ccggacaatg
    13081 ccgccgcgct cgtaaaacga ggcaatcacg gattccagat acgccgggaa ggcttcctcg
    13141 cccggtattt cctccaggcg gccggacact tcgcggaggg cctgcgccca gcgcgacgtg
    13201 gagtcggcca gcagcagcac atgcagcccc atctggcggt aatattcagc aatggtcacg
    13261 gccgtataca ccgaagcctc gcgcgcggcc accggcatgg aactggtgtt gcagatgatg
    13321 agcgtgcgct ccatgaggct cttgcccgtc cgggggtcgg tcagctcagg gaactcgcgc
    13381 agggtttcca ccacctcgcc ggcgcgctcg ccgcaggccg ctatgatcac cacatccaca
    13441 tcggcatagc ggctggtgat ctgctgcagc acggtcttgc cggcgccgaa gggcccgggg
    13501 atgcagtagg tgccgccgcg cgccacgggg aagaaagtgt ccacaatgcg gaccttcgtc
    13561 accagcggct cggtcggacg aaggcgttcg gcatactgcc ggatcgggat cttcaccggc
    13621 cagcgctgat acatcttcac ttccaccaac tgcccctccg catccttcag acgggctatc
    13681 actttttcaa catcgtattc cccggcggcg gaaatttcct ccacggtgag tttccccttc
    13741 aggtggaagg gaaccataat ggcgtgttca aaaatacctt caggcaccgt gcccagggca
    13801 tctgcgggag aaacggttgc gccctttttc acggcgggcg tgaacgccca cttcttcgcg
    13861 cggtcgagcg cctggagata cattccccgc ttcaggaaaa acccggcctc cgcggccagc
    13921 gccggaagcg gattctgaag tccgtcgtag acctgcgaca gcagtccggg ccccagttcc
    13981 accgaaagca gttcacccgt gaactggaca tcatcgccga ccatcaggcc ggtcgtgtcc
    14041 tcgaacacct gcagttcggc gcggctgccg cgaatgcgga tgacttcgct tttcagcttc
    14101 tcgcccttga catgcgcgta gcccacctcg ttctggatga ccggcgaaga aaactccacc
    14161 gtcagcaggt tgccgttcac gcccacaatt ttcccgatgt tttcactcat ggtgttctcc
    14221 acctgtttgc ttcttcgtta aatcttccgc ggcgcccatt ttctgcaggg tctcattggc
    14281 cgtcagtccg gtcagcagtt cagccagccg ggactgccc
//
LOCUS       DAOQWR010000057        14203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4745_length_14203_cov_5.988479, whole genome shotgun sequence.
ACCESSION   DAOQWR010000057 DAOQWR010000000
VERSION     DAOQWR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14203
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4745_length_14203_cov_5.988479"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PKK36_06110"
     CDS             complement(<1..1216)
                     /locus_tag="PKK36_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNR94169.1"
                     /translation="MIEFSNVSKAYGAQVILSGVSFLIGDQERVGLVGMNGVGKSTVF
                     ELIAGTVPADRGSINISGGRRMAHLRQVLKPEHQELSLIAYTETALPEIAELQQEIIE
                     VEHRLHAAEVDKESLLGQLGRLQSQFEAAGGYTIRTRASVTLGGLGFREDQFDRPLAD
                     FSGGWQMRAELARTLTADPDILLLDEPSNFLDVPAVEWLQRFLKQFKGTLILISHDRF
                     LLNSLTGTTLEMAGGDVTRYKGNYDWYVSQRAQRVENAAAALKNQQRKREQMERFVER
                     FRAKNTLATRVKSKIKMIERMEVIEVPVELRTRGRLRLPEPAHSGHEVVRLEDAGYSY
                     DGANWVLRDLNLSIHRGQKIALVGLNGMGKTTLLRMLAGQLVPGEGKRVLGHKVVPGY
                     QSQETTETIPLDL"
     gene            1377..7949
                     /locus_tag="PKK36_06115"
     CDS             1377..7949
                     /locus_tag="PKK36_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR94170.1"
                     /translation="MNKVKPSVLLILVCTLFSLTIPAGAEVMLQWFESDWDEMYQKMP
                     LVAQTGYDWLWIPPPTKGPVGRSVTWANVGYNLYDRFDIGDIPQRGTLETRYGTRGSL
                     NAMIDAAHGLDIKIIPDIVMNHNGNGPDIREYPGMTAEDFHVKWSANYAGNLNYERAP
                     RMNWWYHHGGYGGTMWSDLANLADIRTEDHPLNGDPLRFTGPKTDSDGNSFDFVGSRP
                     DYIRHVGQYDKYPYAYTNELVAEMLYRWIAWLGDAIDYDGLRIDAAKHTPYEFFGWRG
                     AGFLHEAQWNFSQRRGYSFGGDPTDLFQNYVLRTNALLFAEILSPWSEIEYWHGYGSN
                     DRNPMRFLDYAVKKHLDSKFNGDMNGLGSFGSNFDETKGIYYAWGHDEGGASKIDLAY
                     AYILTHIGFPMVYYTGNNITWADSGRAPYPDKRTWMIPGYDSKALGEQYNDIANLVWI
                     HQNFTRGREYKRWENDGDFFALERYDDTVIANGAPDEGEGLVLVALNDSGWDQTRTLN
                     TCFPNGTVLKDYTGKNPNEVTVSGGQVTITVPGNSGQGWVCYAPKIADPVNVRVLQGG
                     SDAPTMTWIIPGGVHQADTTQQITRITSTNFTVETTFTPPAGGSVDNVMLKWGRGVHL
                     PVTNYFSEGRDVVSGHFHSMNRNDATNYSLGITITETNLPEGLNVIKCRAFNQRTAGY
                     SALFNTATKVVYVDRRGPDLSLNVSENQSMVSEFVLIITNSDRTAYGMTVSVNGGAAE
                     AADEVMKGLWKYGITGLSAGTHTAVVTATEADYAASRQVINTSTLTRVFQITDNYEGI
                     GFNHSANAVIELPFFQTVVTGGSESYSSLRIFWDGYELPFNGGNRTNVFNGEILFRDQ
                     TSVVTNRFWGNFINGPHYFEAERVDGIYTSRVVRRVVFSLYGNNHIDSDGDGLPDNVE
                     MPFFDQGAPGPDQPWPGDSNNNFVPESWETWTRLNPYNHATFYGATWDDQLDSDGDGY
                     SNYAEVEAGYAEDGNVYKYSIYDSGSFPSGTPMTASSAGWTPTLAVRGSPLTVTYYPN
                     EGALDNASQVWMHIGHSKKTMGDWQEVYDTNMTVYAGSNWQVTVTVPTNATSVDFTFW
                     DGTSTWDGKDWQANVAGVTNMSFTMDGALDSSDFAVWEDGMWIWAAARGTKLYVATWA
                     SGSGGNDHFLYVTDEPGDAQATPWAKAGYVFLDAAAKPYLAGEGDNSWSGWFNISGNS
                     TNVKGAGAYNYLEGEIDLIDAFGYVPDAVYLASAAYENWDAGALTSQGPHMWDGGDNI
                     DATELLRVPIDSIRDEDADGYFDAGRPQMWTVVNGNTNDANYGMRRFFINELANESRE
                     ITVILQPNAGVGNTVSDVELFSNLNRRDFATLDEDPDSVTVTSGTNYYRAWPMTDIGG
                     GKYRVTVPVNKCGAYRINARYKINGGDYVYYTDNGLRRDCAVVVTPQKALELVMYELN
                     PLIAEATSDSFAGRSTFEDMYRVNTDRPDAVSTSRLAQLGINMVWLQPIHPIGSDNRQ
                     IDPATGLPYDPGSPYAVRNYWKVNSILGDPSSDAQALSEFQDFVGALDNIGVGVMLDG
                     TFNHSAWDCEIGQPAVDMFAWASNASDLIREVRPQWYSKYNSYGQPASYYESAINNDI
                     APAPDRMDFGKWTDAADFYFGTYDALVAGIPSDTNWAWSSGWYSRYLFEEDRFDGFAS
                     GYTRDLWEYFAHYPVYWLEKTGHPDGTPKSESYKGIDGLRCDFAQGLPSEFWEYTINK
                     TRSVKWDFLFMAESLDGYREVGGSKRHGVGYRSARHFDILNENMVFYWRDNFFDYFGS
                     GGQPQTYPTWQAFDNRRNAFDVVPILLNLTSHDEIYPTDDQWRLIYAHSIVSAWDGIP
                     MMVYGQEAAAQNDASTYTNRGINPANNFARYETNFGKSIPNFKRYNHMTNIWNGLDSS
                     WKTSMYETYQRINNARLGSPALRSQQNYMLEGTNGWNPDIFCTIKYQAPGISAASQDV
                     VIVAINNNYAGSTNRWDNFNLDVDYTPGVNWFGIQDSHYYNVVDLMGANPTNYLWAEP
                     GQLGSAIKADFWVLLNGNPFEGQQAQYLRLIDRTAGNTPETVVNYFAWDRDGDGLPDD
                     WESANSLDPNSSSDDDGAAGDPDDDDMTNAQELLAGTDPQTKDALELGIDVAGSAVDV
                     WWNSITDINYILQRSDSLTSGPGWETIYFGTAMDTNAIVTDTTSGSVTSRYYRAVVVP
                     "
     gene            complement(7992..9194)
                     /locus_tag="PKK36_06120"
     CDS             complement(7992..9194)
                     /locus_tag="PKK36_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR94171.1"
                     /translation="MLSDRPFRDLNSSQPSVHVAAGQGGYANIRAALNGFDLSAARGK
                     KVLLKPNAGRMAAAGSGIVTGPEAVAAAADAFLEAGAEVAVGESPILGVRTMDAFEQC
                     GIAAVARERKLTLIDLDARPFVPVPVPGGRAIGELRVCPEIMEYDFVISLPVMKMHMH
                     TGVTLSVKNMKGCLWRRSKVDLHMLPPVEGSADKSLDIAIADMSGVLRPHLAVIDGYI
                     GMEGLGPSAGTPRKLDAAVVSADPFAADAVACRLMGLSAREVPHLRIGAERGYGLIDL
                     DRMSISPDNWMDYASPFERPPRDLALEFPGITILDRNSCSSCQSTLLLFLRRYGEKIF
                     DYFPKELQPVIAIGKGHDEVPRGTLCLGNCTARHRSQGIFVPGCPPVASAIFKAIGGD
                     ADREDDEV"
     gene            9278..10756
                     /locus_tag="PKK36_06125"
     CDS             9278..10756
                     /locus_tag="PKK36_06125"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNR94172.1"
                     /translation="MSRLYPAHADLFEFDAAFPRAKAYRSDGRAAGLVREACTAARSN
                     ETFSERHLQCIWFDPSLRPQNLKTAEGESVRVVQPGSWNLEAGPDFLRAAVEVGGSRL
                     IEGDAEVHIRPADWNRHGHQNDPRYRHVRIHITFYAPGEAALPPGCIEISMQKPLLAN
                     PSFSFQQIDLTAYPYAARGKLTACAAVLSGLPPEAGAALLDAAGEERLRRKTAWFAHL
                     LSTCEPEDVLYRELMGALGYKHNSAAFRTLADHVPLPRLREAAGGRAHAAYAILLGCA
                     GLLPKELPARSPECRDFVRKIWDVWWKVNSQWADSIMPGAAWKLSGLRPANHPVRRLM
                     AAAHLFTREEPLSQRLRRAAGTIDDMRELLNFPSGNFWDYYLSFNGQRQAAPVALIGD
                     SRTAAIIINIFIPWLAARGELPVPAAGMLDSLPGGTADSTVKMTAHNLFGPAHSRKLY
                     SSGLRRQGLIQIFNDFCVQDRSSCANCKFPEIIKRNFGTAII"
     gene            10782..11192
                     /locus_tag="PKK36_06130"
     CDS             10782..11192
                     /locus_tag="PKK36_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR94173.1"
                     /translation="MENKIIYKEESYKIIGACFEVYREKGNGFLEAVYQECLAIEFSD
                     QGIPFVEKPKLALSYKAKALKQYYEPDFVCYDRIIVEIKAVKILADEHRAQTINYLKS
                     TGFELGLLLNFGHYPKLEQERFPNQNISRLSRIS"
     gene            complement(11193..12530)
                     /gene="rlmD"
                     /locus_tag="PKK36_06135"
     CDS             complement(11193..12530)
                     /gene="rlmD"
                     /locus_tag="PKK36_06135"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNR94174.1"
                     /translation="MSLSAGDQVELQIHDVAQDGRWVGRGASGQVVFVRGEAVPGERV
                     RARVAVVKKSFVEADLLAVLEKAPSRIKPPCPYFGKCGGCQLQHIRYSAQLDLKKKMV
                     CDALERLGRFDRPAVLNVMPSEAEYGFRNKLELVFTKNGRLAFHGYGDGRPLVAVERC
                     LLAPDILNRLMDVTARFFSGLPAAERQACGLRRLMLRRADMDGRVMVALELRRAPEQT
                     MQNCLKSLLRVSGSDRTTVVTEIGEERRPLKQAVLHGPGVLEEKLGRYKFIIGPDSFF
                     QTSTRQAEKLCETVAGMARGGKQNLALDLFCGTGPLAHCLSESHADVIGVDGQRSAVE
                     NAEANARLNGRANIRFVAHALDRIADLGTAARPDLVVMDPPRNGIHADALRGIMRLGA
                     EKIIYVSCNPATFARDARLLCEDSRYRLVRVQPIDMFPQTGHIETVSLFQNKN"
     gene            complement(12533..12979)
                     /locus_tag="PKK36_06140"
     CDS             complement(12533..12979)
                     /locus_tag="PKK36_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNR94175.1"
                     /translation="MILDCLDNIAECRELHPALAEALDFIRSADLAALSPGRHEVRDD
                     RIYVSVSRGPGRARDDGLLEVHRKYIDIQIVLDGREEMGWKPLSRCARPEAGYDAERD
                     IQFFQDEPDSWMTVRPGQFAVFFPWDAHLPLVSAGIVHKAVVKVGV"
     gene            complement(13053..>14203)
                     /locus_tag="PKK36_06145"
     CDS             complement(13053..>14203)
                     /locus_tag="PKK36_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNR94176.1"
                     /translation="LLWRPSPLCRARALEKALGTPARIYYKNEGVSPAGSHKPNTAVA
                     QAYYNKVFGINRLTTETGAGQWGCALSFACSLFGMHCRVYMVRISFDQKPYRKLMMQA
                     WGGECLPSPSENTEVGRRILDDDPDTPGALGIAISEAIEDCVKSKDTRYSLGSVLNHV
                     LLHQTIIGLEAQKQMEKISEYPDVVIGCCGGGSNFAGISFPYIRDKIHGKNLRVVGVE
                     PTACPTLTKAPYVYDHGDTAGMTPLLAMHSLGHTFVPKPIHAGGLRYHGMAPLVSHVL
                     REGLMEAAAIHQIECYEAAVLWARTEGHIPAPETSHAIAQAIREAKRAKEEGKEKVIL
                     FNWSGHGLMDLAGYDAYLQGHLHDYHLSDEDMKKSISSTESYPKPPSL"
BASE COUNT         3036 a         4366 c         4133 g         2668 t
ORIGIN      
        1 tcaaatcgag cggtatggtt tcggtggttt cctgggactg atagccgggc acgactttgt
       61 gaccgagcac gcgcttcccc tcgcccggca caagctgtcc ggcgagcatg cgcagcagcg
      121 tggttttacc catgccgttg agtcccacga gcgcgatttt ctgaccgcga tggatgctga
      181 gattgagatc gcgaagcacc caattggccc cgtcatagga atagccggcg tcttccagac
      241 ggaccacttc atgcccgctg tgggcgggct cgggcaggcg caaacggccg cgggtccgca
      301 gttcaacggg cacttcgatt acctccatgc gctcgatcat cttgattttg cttttgacac
      361 gcgtggccag cgtgttcttg gcacggaagc gctcgacaaa gcgttccatc tgctcacgct
      421 tgcgctgctg gttcttgagc gcggcggcgg cattttccac gcgctgcgcg cgctggctga
      481 cataccagtc gtagtttccc ttgtagcgcg taacgtctcc ccccgccatt tcaagcgtgg
      541 tgccggtcag ggagttgagc aggaagcggt cgtgcgaaat cagaatcagc gttcccttga
      601 actgcttcag gaagcgctga agccattcaa cggcgggcac gtcgaggaag ttggagggct
      661 catcgagaag caggatgtcc ggatcagcgg tgagggttcg cgccagttca gcgcgcatct
      721 gccagcctcc gctgaaatcg gccagcgggc ggtcgaactg gtcttcccga aagcccagtc
      781 cgccgagcgt gacggaggcg cgggtgcgga tggtgtatcc cccggccgcc tcgaactgcg
      841 actggagccg gcccaattgc cccagaaggc tttccttgtc cacctccgcg gcgtgcagcc
      901 gatgctccac ctcgatgatt tcctgctgca gttcggcgat ctcgggcagg gcggtctcag
      961 tgtaggcaat cagcgaaagc tcctgatgct cgggtttgag cacctggcgc agatgcgcca
     1021 tcctgcggcc gccggatatg ttgatggagc cgcggtcggc gggcaccgtg cccgcaatca
     1081 gttcgaacac ggtgcttttt ccgaccccgt tcattccgac gagaccgacg cgctcctgat
     1141 cgccgatcag gaaagaaact ccggagagga tcacctgggc gccataggcc ttgctgacat
     1201 tgctgaattc gatcattgga gcgggactat tgcaaatttg gcggcgggcg gcgaagcaaa
     1261 ataaaaaagt gaatcttttt tgactttggg cttaacagga gcgaaagatt atcatacaag
     1321 gtgaaggtaa agcgtttgac cgggttggtc ttttttacag cgatcaggag ctcgtcatga
     1381 acaaagttaa accgtctgtc ctgctgattc tcgtctgcac acttttctcg ctgaccatcc
     1441 cggccggcgc ggaggtcatg ctccagtggt ttgaatccga ctgggatgaa atgtaccaga
     1501 aaatgccgct ggtcgcccag accggctatg actggctctg gatccccccg cccaccaagg
     1561 ggcccgtggg ccgcagcgtc acctgggcca atgtcggcta caatctgtac gaccgctttg
     1621 acatcggcga catcccccag cgcggcacgc tcgaaacgcg ctatggaacc cgcggctcgc
     1681 tcaacgccat gattgatgcg gcgcacgggc tggatatcaa gatcattccc gacattgtga
     1741 tgaaccacaa cggcaacggg ccggacatcc gcgagtatcc gggcatgacg gccgaagact
     1801 tccatgtcaa atggtcggcc aattacgccg gcaacctcaa ctatgaacgg gccccgcgaa
     1861 tgaactggtg gtatcatcac ggaggatacg gcggcacaat gtggtcggac ctcgccaacc
     1921 tcgccgacat ccgcacggag gatcatccgc tgaacggcga tccgctgcgc ttcaccggtc
     1981 ccaagacgga ctccgacggc aactccttcg attttgtcgg cagccggccg gactacatcc
     2041 gccatgtcgg ccagtacgac aaatatccct atgcctacac gaatgagctg gtggcggaga
     2101 tgctctatcg ctggatcgcg tggctgggcg atgcgattga ttacgacggg ctgcgcatag
     2161 acgcggccaa gcacacgccc tatgaatttt tcggatggcg cggcgcaggc ttcctgcacg
     2221 aagcccagtg gaacttctcg cagcgccgcg gctacagctt cggcggggac cccaccgatc
     2281 ttttccagaa ttatgtcctg cgcaccaacg cgctgctgtt cgcggaaatc ctgagcccgt
     2341 ggtcggagat tgagtactgg cacggctacg ggagcaacga ccgcaatccg atgcgcttcc
     2401 tggactatgc cgtcaaaaag cacctcgaca gcaaattcaa cggcgacatg aacgggctgg
     2461 gctcgtttgg atcgaacttc gatgaaacca agggcatcta ttacgcatgg gggcatgatg
     2521 aaggcggagc gagcaaaatc gaccttgcct atgcctacat cctgactcac atcggcttcc
     2581 cgatggtata ttacaccggc aacaacatca cgtgggccga cagcggccgc gccccctatc
     2641 ccgacaagcg gacctggatg attccgggct atgacagcaa ggccctcgga gagcagtaca
     2701 acgacatagc caatctggtc tggattcacc agaacttcac acgcgggcgc gaatacaagc
     2761 ggtgggaaaa cgacggcgac ttcttcgcgc tggagcgcta tgacgacacg gtgatcgcca
     2821 acggagcgcc ggatgagggc gagggcctgg tgctggttgc gctgaacgac tcgggctggg
     2881 accagacccg aacgctgaac acctgcttcc ccaacggaac cgtccttaaa gactacaccg
     2941 gaaagaatcc aaacgaagtc accgtgagcg gcgggcaagt caccatcacc gtgccgggca
     3001 acagcggaca gggctgggtc tgttatgccc cgaaaatcgc cgatcccgtc aatgtccgcg
     3061 tgctgcaggg cggaagcgac gccccaacga tgacatggat cattcccggc ggcgtccatc
     3121 aggccgacac cacgcagcag atcaccagaa tcacctcgac caacttcacc gtagaaacca
     3181 cgttcacgcc gccggccgga ggatcggtgg acaacgtaat gctgaaatgg gggcgcgggg
     3241 tgcatctgcc ggtcaccaac tatttcagcg aaggccggga cgtcgtcagc ggccatttcc
     3301 acagcatgaa ccgcaacgac gccacaaact attcactggg catcacgatc accgaaacca
     3361 atctgccgga aggcctgaac gtcatcaagt gccgggcctt caatcagcgc acggcgggct
     3421 attcggccct gttcaacacc gcgaccaaag tggtctacgt ggaccgccgc gggccggatc
     3481 tttcgctgaa cgtttccgaa aaccagtcca tggtcagtga atttgtgctg atcatcacca
     3541 acagcgaccg gacggcttac gggatgaccg tatccgtgaa cggcggcgcg gccgaggcgg
     3601 ccgatgaggt gatgaagggc ctctggaagt acggcatcac cggcctgagc gccggaacgc
     3661 atacggccgt ggtcaccgcc accgaggccg attatgcagc gagccgacag gtcatcaaca
     3721 ccagcaccct gacccgcgtt ttccagatca cggacaacta cgagggcatc ggattcaacc
     3781 attcagcaaa cgcggtgatt gaacttccct tcttccagac cgtggtcacc ggcgggagcg
     3841 agtcctacag ttcgcttcgc atcttctggg acggctatga gctgcccttc aacggcggaa
     3901 accgcacgaa tgtgttcaac ggggaaattc ttttccgcga tcagacctct gttgtgacca
     3961 accgcttctg gggcaatttc atcaacggtc cgcactactt tgaggccgag cgggtggacg
     4021 ggatatacac cagccgcgtg gtgcgccgcg tggtgttcag cctgtacggg aacaaccaca
     4081 ttgactcgga cggcgacgga ctgcccgaca acgtcgaaat gccgttcttc gaccagggcg
     4141 cgcccggacc cgaccagcca tggcccggtg acagcaacaa caacttcgtt cccgagtcat
     4201 gggaaacatg gacccgcctg aatccgtaca atcatgcgac attctacggc gccacatggg
     4261 acgaccagct ggattccgac ggcgacggct acagtaatta cgccgaagtc gaggccggtt
     4321 atgctgaaga cggcaacgtc tacaagtaca gcatctatga ctccggcagc ttcccgagcg
     4381 gaaccccaat gaccgcatcc tccgcgggct ggacccccac tctggccgtg cggggcagtc
     4441 cgctcacggt cacctactat ccgaacgaag gcgcgctgga taatgcgtcg caggtctgga
     4501 tgcatatcgg ccattccaaa aagaccatgg gcgactggca ggaagtgtac gacaccaaca
     4561 tgaccgtcta tgccgggagc aactggcagg tcaccgtcac ggtgccgacc aacgcgacat
     4621 ccgtggattt cacgttctgg gacggcacca gcacatggga cgggaaggac tggcaggcca
     4681 atgtcgccgg cgtcaccaat atgtcgttca ccatggacgg cgcccttgac agctccgact
     4741 ttgcggtctg ggaagacggc atgtggatat gggcggccgc gcgaggaacc aagctgtatg
     4801 tggccacatg ggcttcggga agcggcggca acgatcactt cctgtacgtg acggacgaac
     4861 ccggcgatgc tcaggccaca ccctgggcca aggccggtta tgtcttcctg gatgcggccg
     4921 ccaaacccta cctcgccggc gaaggcgaca acagctggtc cggatggttc aacatctcgg
     4981 ggaacagcac gaatgtcaaa ggggccggag catacaacta ccttgagggt gagattgatc
     5041 tcatcgacgc gttcgggtat gtgcccgacg ccgtctatct ggcttcggcc gcttatgaaa
     5101 actgggacgc cggagcactg accagccagg gcccccacat gtgggacgga ggagacaaca
     5161 tcgacgccac ggagcttctg cgcgtcccca ttgacagcat tcgcgatgaa gacgccgacg
     5221 gttattttga cgccggacgg ccgcaaatgt ggaccgtggt caacgggaat accaacgacg
     5281 ccaattacgg aatgcggcgg ttcttcatca acgaactggc gaatgaaagc agggaaatca
     5341 ccgtcattct gcagccgaac gccggtgtcg gaaacacggt cagcgacgtg gagctttttt
     5401 ccaacctgaa ccggcgcgac ttcgcgacgc ttgacgaaga tccggacagc gtaaccgtca
     5461 caagcggcac caactattat cgcgcatggc caatgaccga catcggcggc gggaaatacc
     5521 gcgtgaccgt ccccgtgaac aaatgcggcg cctatcgcat caacgcccgc tacaaaatca
     5581 acggcggcga ctatgtctat tacacggaca acggattgcg gcgcgactgc gccgtggtag
     5641 tcacgccgca gaaggcgctg gaactcgtga tgtacgagct gaatccgctg atcgctgaag
     5701 ccaccagcga cagcttcgcc ggccgcagca cctttgaaga catgtaccgc gtgaacacgg
     5761 accggccgga tgccgtcagt accagccggc ttgcacagct gggcatcaac atggtctggc
     5821 tgcagcccat tcacccgatc ggctccgaca accgccagat tgatccggcc accggcctgc
     5881 cctatgatcc cggaagtccc tatgcggtcc gcaactactg gaaggtgaat tccatcctcg
     5941 gcgatccttc cagcgacgcg caggccttgt cggaattcca ggacttcgtg ggtgcgctcg
     6001 ataacatcgg cgtgggcgtg atgcttgacg gcaccttcaa ccacagcgca tgggactgcg
     6061 agatcggcca gcccgccgtg gacatgttcg cgtgggcctc taatgcatca gacctcatcc
     6121 gcgaggtccg gccgcagtgg tattccaaat acaacagcta cggccagccc gcatcctact
     6181 acgagagcgc catcaacaac gacatcgcgc ccgcgccgga ccgcatggac ttcgggaaat
     6241 ggaccgatgc ggccgatttc tattttggca cctacgacgc gctggtggcc ggcattccat
     6301 ccgacaccaa ctgggcatgg tccagcggat ggtacagccg ctatctcttt gaggaggacc
     6361 gtttcgacgg attcgcgtcg ggctatacac gggacctgtg ggaatacttc gcccactatc
     6421 cggtctactg gctcgagaaa acgggccatc cggacggcac gccgaagagc gaatcctaca
     6481 agggcattga tggccttcgc tgcgacttcg cgcaggggct tccgtccgaa ttctgggaat
     6541 acaccatcaa caagacccgc agcgtgaaat gggatttcct gttcatggcg gaatcgctgg
     6601 acggctaccg cgaagtgggt ggcagcaagc ggcacggagt gggttaccgc tcggcccggc
     6661 acttcgacat cctgaatgag aacatggtgt tctactggcg ggacaacttc tttgattatt
     6721 tcggctcggg cgggcagccg caaacctatc cgacctggca ggcgttcgac aaccggcgca
     6781 atgcgtttga cgtggtgccg attctgctga acctgaccag ccatgacgag atttatccca
     6841 cagacgacca gtggcgcctg atctacgccc attcgatcgt cagcgcatgg gacggcattc
     6901 cgatgatggt gtatgggcag gaggccgccg cgcagaacga tgcgtccacc tacaccaacc
     6961 gcggcatcaa ccccgccaac aactttgcgc gctatgagac caatttcggg aaatccattc
     7021 cgaacttcaa gcgctacaac cacatgacaa acatctggaa cggcctggac tcgtcgtgga
     7081 agacctccat gtacgaaacg taccagcgca tcaacaacgc gcggctcgga tcccccgccc
     7141 tgcgcagcca gcagaactat atgctggaag gcaccaacgg ctggaatccc gacatcttct
     7201 gcaccatcaa gtatcaggcg cccggaatta gcgcggcctc gcaggatgtg gtgattgttg
     7261 caatcaacaa caactatgcc ggaagcacca accgctggga caacttcaac ctcgatgtgg
     7321 actacactcc gggggtgaac tggttcggca tccaggactc gcactactac aacgtcgtgg
     7381 acctgatggg cgccaatccc acaaactatc tctgggcaga accgggacag ctcggttcag
     7441 cgatcaaggc ggatttctgg gtgctcctca acggcaatcc gttcgagggc cagcaggcgc
     7501 agtatctgcg gctgattgac cgcacggccg gcaacacgcc ggagacggtg gtgaactact
     7561 tcgcatggga ccgtgacggt gacggactgc ccgacgactg ggaatccgca aacagtctcg
     7621 acccgaacag ctcatccgac gatgacggcg ccgcgggcga tcccgacgat gacgacatga
     7681 ccaacgccca ggaactcctt gcgggaaccg atccccaaac gaaggatgcc ctcgaactgg
     7741 gcattgatgt ggccggcagc gcggtggatg tctggtggaa cagcatcacg gacatcaact
     7801 acatcctgca gcgcagcgac agcctgacca gcgggcccgg gtgggaaacg atctacttcg
     7861 gcacggcgat ggacacaaac gccatcgtca ccgacaccac ctccggcagt gtgacgagcc
     7921 gttattatcg cgccgtggtg gtgccgtaag gcattcaaat cccgtccgcc ggcagcgccg
     7981 gcggatggga ttcaaacttc atcatcctca cgatccgcat ccccgccgat cgccttgaat
     8041 atggcgctgg ccacaggcgg gcagccgggc acgaatatcc cctgcgacct gtgccgcgcc
     8101 gtgcagttgc caaggcaaag cgttccccga ggaacttcat cgtgcccctt cccgatggca
     8161 ataacgggct gcagctcctt cgggaaataa tcgaagattt tttctccgta ccggcgcaaa
     8221 aagagcagaa gtgttgactg gcaggaactg caggagtttc tgtcgagtat ggttattccg
     8281 ggaaattcca gcgcaaggtc ccttggcggc cgctcaaagg gagacgcata atccatccag
     8341 ttgtccggag atatgctcat gcggtcgaga tcaatcagcc cgtatccccg ctcggcgccg
     8401 atgcgcagat ggggcacttc ccgggcggag agacccatca ggcggcaggc caccgcatcc
     8461 gcggcaaacg gatcggcgct tacgacggcg gcatccagct tcctgggggt tcccgccgaa
     8521 gggccaagcc cctccatgcc gatgtatccg tcaatcaccg ccagatgcgg ccggagcacg
     8581 ccggacatat cggcaatcgc gatatccaga gacttgtctg cggacccctc gaccggcggg
     8641 agcatgtgca ggtccacctt gctcctccgc cagagacacc ccttcatgtt tttcacggac
     8701 agcgtgaccc ccgtgtgcat gtgcattttc atcacgggaa gcgaaatcac aaagtcgtac
     8761 tccattattt cgggacagac acgcagttct ccgatggccc ggccgccggg cacgggaacc
     8821 ggaacaaagg gcctcgcatc aagatcaatg agggtcagtt ttctttcacg cgccacggcc
     8881 gcaatgccgc actgctcaaa cgcatccatt gtcctgacgc ccagaatggg actttcgcca
     8941 acggccactt cggcgccggc ttccagaaat gcgtccgcag cggcggccac ggcctcggga
     9001 ccggtgacaa ttcccgaccc ggcggcggcc atgcggcccg cattgggttt cagcagcact
     9061 tttttgcctc gcgccgcgga aagatcaaaa ccattgaggg ccgcgcggat gttggcatag
     9121 ccgccctgcc cggccgccac atgaacagag ggctgcgatg aattcagatc ccgaaatgga
     9181 cggtcagaca acatgcccga atctaatccc cgggccgccc gcatgcaagg cgggattccc
     9241 ttgccgtcac ggcatgtttt cggctatgaa tctattcatg agccggctct atccagcgca
     9301 tgccgatctc tttgagtttg atgcggcatt cccccgcgca aaagcatacc ggtcggacgg
     9361 ccgcgccgcc ggcctggtgc gggaagcctg cacggcggca cggtcgaatg aaacattctc
     9421 cgaacgacac cttcagtgca tctggttcga tccctccctg aggccgcaga acctgaaaac
     9481 cgccgaaggc gaaagtgtgc gggtggtgca gcccggaagc tggaacctgg aggccgggcc
     9541 cgacttcctg cgcgcggccg tggaggtggg cggatcccgc ctgatcgaag gcgacgcgga
     9601 ggttcatatc cggcccgccg actggaaccg tcacggtcat caaaacgatc cgcgttatcg
     9661 tcatgtgcgg attcatatca ccttttacgc cccgggagaa gcggcgcttc cgccgggatg
     9721 cattgaaata tcaatgcaga agcccctgct ggcgaacccc tcattttcct tccagcagat
     9781 tgacctgacg gcctatccgt atgcggcgcg gggaaaactc accgcatgtg ccgccgtgct
     9841 gagcggcctt cccccggagg ccggggccgc gctgctggac gcggcggggg aagagcgtct
     9901 gcgccggaag acagcctggt ttgcgcacct gctgagcacc tgcgagccgg aagatgtcct
     9961 ttatcgcgaa ctgatgggcg cgctgggcta caagcataac agcgcggcct tccggacgct
    10021 ggcggatcac gtacctcttc cgcgactgcg cgaagccgcc gggggccgcg cacatgctgc
    10081 ttacgccatc cttctcggct gtgccggtct gctgccgaag gagctgcccg cgcgcagtcc
    10141 ggagtgccgg gattttgttc gaaagatctg ggatgtctgg tggaaggtca acagccagtg
    10201 ggccgacagc atcatgccgg gtgccgcatg gaagctttcc gggcttcgcc ccgccaacca
    10261 tccggtgcgg cggctgatgg ccgccgcgca tttgttcacg cgggaggagc cgctctcgca
    10321 gagactgcga cgcgcggcag gcacgattga cgacatgcgg gaacttctca actttccttc
    10381 cgggaacttc tgggattact atttgtcctt caacggacag aggcaggcag cgccggtggc
    10441 cctgattgga gattccagaa cagcagccat cataatcaac atcttcatcc cgtggctggc
    10501 cgcgcgcggc gagcttccgg ttccagcggc aggcatgctg gacagtcttc cgggcggtac
    10561 agcagacagc accgtgaaaa tgaccgcgca caacctgttc ggacccgccc acagccgcaa
    10621 actgtacagc agcggcctgc gccggcaggg tttgattcaa atattcaacg acttctgtgt
    10681 tcaggaccgt tccagctgcg caaactgcaa attccccgaa attatcaaac gcaatttcgg
    10741 aacagcaatt atttaactac gaaaaacgcg aaaggcgcga aatggaaaac aagattatat
    10801 acaaagagga gagttacaaa atcattgggg catgttttga agtgtatagg gaaaagggga
    10861 acggatttct tgaagcggtg tatcaggaat gcctcgcaat agaattttcg gatcagggaa
    10921 ttccgtttgt tgaaaaaccc aaactggctc taagttataa agctaaggcg ctgaagcagt
    10981 attatgagcc ggactttgtc tgttatgatc gcatcattgt tgagatcaaa gccgtgaaaa
    11041 tattagccga tgaacaccgg gctcaaacaa tcaattattt gaagtctaca ggtttcgaat
    11101 taggactctt actgaacttt ggacattacc cgaaactgga gcaagagcgt tttccgaatc
    11161 agaacatttc gcgcctttcg cgtatttcgt agttaattct tattctgaaa gagagagacg
    11221 gtttcgatgt gaccggtctg gggaaacatg tcaatcggct ggacgcgaac aagacgatag
    11281 cggctgtctt cacacagcag tcgcgcatcg cgcgcgaagg tcgccggatt gcagctcaca
    11341 tatatgattt tttccgcgcc cagacgcatg atgccgcgca gggcgtccgc atggattccg
    11401 ttgcgcggag gatccatgac gaccagatca gggcgcgccg cggtgcccag atcagctatc
    11461 cggtccagtg catgcgccac gaaacggatg ttcgcgcggc cgttcaggcg ggcgttggct
    11521 tccgcatttt caacggcgga cctctgaccg tccacgccaa tcacatcggc atgcgactcc
    11581 gacaggcaat gcgccagggg gccggtcccg cagaagagat ccagcgcgag gttctgtttt
    11641 ccgccccggg ccatgccggc gaccgtttcg cagagttttt ccgcctggcg cgtggaggtt
    11701 tggaagaagg aatccggtcc gataatgaac ttgtaccgtc ccagtttctc ttccagcact
    11761 ccggggccat gcaggaccgc ttgcttcaac ggccggcgct cctctccgat ttcggtcaca
    11821 accgtggtgc ggtcgctccc gctcacccga agaaggcttt tcagacagtt ctgcatggtc
    11881 tgttcgggcg cgcggcgcag ctcaagagcg accatgaccc ggccgtccat atccgcccgc
    11941 cgaagcatca ggcgccgcaa gccgcaggcc tgccgttccg cagccggaag accggaaaaa
    12001 aagcgggcgg ttacatccat aaggcggttg agaatatcgg gggccaggag acagcgctcc
    12061 acggcaacaa gcgggcggcc gtcgccatac ccgtgaaaag ccagccggcc gtttttagtg
    12121 aacaccagtt ccagcttgtt gcgaaacccg tactctgcct ctgagggcat gacattcagc
    12181 accgccggcc ggtcgaaacg gccgagacgc tccagcgcat cacacaccat ctttttcttt
    12241 aaatccagct gagcggaata acgaatatgc tgaagctggc atccgccgca tttcccgaaa
    12301 tacgggcagg gcggcttaat gcgcgacggc gccttttcga gcacggccag gaggtcggcc
    12361 tccacaaatg attttttgac aacagccacg cgggcgcgga cccgttcgcc gggaaccgcc
    12421 tccccgcgga caaaaacaac ctgtccggaa gcgccgcgac cgacccagcg gccgtcctgc
    12481 gcaacatcgt gaatctgaag ttcaacctga tcgcctgcgg aaaggctcat gttcataccc
    12541 ccaccttcac gaccgccttg tgaacaatgc ccgccgacac cagcggcaga tgcgcgtccc
    12601 acgggaaaaa aacagcaaac tggccgggcc ttacagtcat ccaggaatcg ggttcatcct
    12661 gaaaaaactg gatatcgcgt tccgcatcat acccggcttc cggccgcgca cagcgggaaa
    12721 gcggtttcca gcccatttct tcccgcccat ccagaacgat ttgtatgtct atgtattttc
    12781 tgtggacttc aagcagtccg tcatcgcgcg cgcggccggg cccccggctg accgagacat
    12841 agatgcggtc atcccggacc tcgtgccggc ccggagacag cgccgccaga tccgctgagc
    12901 ggatgaagtc cagcgcctcc gccagcgccg gatgaagttc ccggcattcc gcgatgttat
    12961 caagacagtc cagtatcatg gcattccgcg ctttctcaaa aagaacagcc gctgcggaca
    13021 gcggctgttc gtcaattatc tcccggcgtc cctcacagag acgggggttt cggatagctt
    13081 tcggttgagc tgatggattt cttcatgtcc tcatcactca gatgatagtc atgcaaatga
    13141 ccctgcagat aggcgtcgta gcccgcaaga tccatcagtc cgtgtcccga ccagttgaaa
    13201 aggatcacct tctcctttcc ttcttctttc gcgcgcttgg cctcgcgaat ggcctgtgcg
    13261 atggcatgag aggtttccgg tgccgggata tggccttccg tccgcgccca gagcaccgcg
    13321 gcctcatagc actcaatctg atgaatggcc gccgcctcca tcagcccctc gcgcagcaca
    13381 tgactgacca gcggcgccat gccgtgatag cgcagtccgc cggcgtgtat gggcttgggc
    13441 acaaaggtat gacccaggct gtgcatggcc agcagcggcg tcattccggc cgtgtcgccg
    13501 tggtcgtaca catagggcgc cttggtcagg gtcggacagg cggtgggctc cacaccgaca
    13561 acacgcaggt tctttccgtg aatcttgtca cggatatatg ggaagctgat gccggcgaaa
    13621 ttggatccgc cgccgcagca gccgatgacc acatccggat attcactgat cttctccatc
    13681 tgcttctgcg cttcgagccc gatgatggtc tggtgcagca gcacatgatt gagaacgctt
    13741 ccaagagaat agcgcgtgtc cttcgacttc acacagtcct caatcgcttc gctgatcgcg
    13801 atgccgaggg cgcccggcgt gtccggatca tcatccagaa tcctgcgtcc cacttcggtg
    13861 ttctcgctcg ggctgggaag gcattccccg ccccatgcct gcatcatcag tttgcgatac
    13921 ggtttctgat cgaagctgat ccgcaccatg tacacgcggc agtgcatgcc gaacagcgaa
    13981 caggcaaaag agagggcgca gccccactgc cccgcgccgg tctcggtggt cagccggttg
    14041 atgccgaaga ccttgttgta ataggcctgc gccacggcgg tgttcggctt gtggctgccg
    14101 gcgggcgaga cgccttcatt tttgtagtag attcgggctg gagtgcccag ggccttttcc
    14161 agcgcgcgcg cgcggcagag cggactcggc cgccacagca gca
//
LOCUS       DAOQWR010000058        14158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4771_length_14158_cov_5.732256, whole genome shotgun sequence.
ACCESSION   DAOQWR010000058 DAOQWR010000000
VERSION     DAOQWR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_14158_cov_5.732256"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKK36_06150"
     CDS             <1..464
                     /locus_tag="PKK36_06150"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HNR94177.1"
                     /translation="MNPATGSPNGFAVSIFSAPSDDSPRDYLGSLAGSAAPVAGGLYT
                     YTASGITIPGTGFYFVLVTSSTPTNQGAFNWSASDSFTHNGSWYINSAYYTSSDGLNW
                     QKHIRDEVFQMAIHATIVPEPATGWLLGVGLLSLSLCRRRLAPEFCIFHRA"
     gene            542..1285
                     /locus_tag="PKK36_06155"
     CDS             542..1285
                     /locus_tag="PKK36_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94178.1"
                     /translation="MQKNAAKKKSGKDQPADVKERLANFALLFELDNLAVNVRELSFE
                     ILKTLLAKNDCKLAEVHFCRYGLAAAPENYLPALLQALDVKKPSAEKLAKDLYKALGE
                     QLAGGKTVLNAALDAVLAETAGLGIRMAAISALPEDMAHALAEKLGLADRGVELFVFS
                     EPEDVFPRADTWLKVAKSMSVPPRHCKVLSTSMASCKAALSTGMNSVAVPDRFTSFHD
                     FGGATMVLDALGDVSATDLVAEIFPLEDL"
     gene            1290..2072
                     /gene="mazG"
                     /locus_tag="PKK36_06160"
     CDS             1290..2072
                     /gene="mazG"
                     /locus_tag="PKK36_06160"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNR94179.1"
                     /translation="MEERPIDRLLGIMRRLRADDGCPWDRKQTVDSLKTSVLEEAYEV
                     VDAIESGRREPLCEELGDLLMLIVFLSQICDEEKAFTFEDVATGICEKLVRRHPHIFG
                     DVKVANADEVIRNWNAIKQTEKAEKKSIIGNIPRHLPALQKAHHVQKRAAQAGFDWDE
                     IRDVVAKLDEELAEVKEAMASQNGKKIAEELGDLLFSVVNVSRFLGHDAEAALNATVD
                     KFIRRFHAVEKGVQEQGREMTDCTLAELDVFWDAAKAQEGTD"
     gene            complement(2054..2440)
                     /locus_tag="PKK36_06165"
     CDS             complement(2054..2440)
                     /locus_tag="PKK36_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNR94180.1"
                     /translation="MEKANQSESASNPLCGKCPALCCRYVAIEIQAPSAKEEFDHIRW
                     YLLHRDVQVFIDHDGAWFVEFVTPCEELTADNQCRIYDRRPEICRSHGNPPEVCEMQG
                     TPYVELFHAPEEFDEYLRKKNQSVPS"
     gene            2546..3760
                     /gene="tyrS"
                     /locus_tag="PKK36_06170"
     CDS             2546..3760
                     /gene="tyrS"
                     /locus_tag="PKK36_06170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR94181.1"
                     /translation="MKNKEVERQISILADRTVDLHSVHELRGKLERSLAENRPLRVKL
                     GADPSAPDIHLGHVVPLNKLREFQDLGHLVVFIIGDFTGMIGDPSGKSATRPQLTREQ
                     VMANAKSYQEQVFKVLDPEKTEIRFNSEWHAPMTFEDVIRLSAHVTVAQILARDDFAK
                     RYAASQPISLVEFLYPLVQAYDSVMIRADVELGGTDQLFNLLLGRELQKTYSQEPQIV
                     MTLPLIEGLDGVNKMSKSLGNYIGVNEPAREIYGKAMSVSDALMWRYFSLILCMPQDE
                     IAEIKKAVETGARHPRDVKDELARKLVAKFHDEAAAAQASDEFKRIFSQNQLPDDVPE
                     VAMAGEKSGIVDLLVNAKLAPSRGEARRLIEQGGVKIDDEKVSDIHAEIPLKTGMIIR
                     AGKRKFAKIVLS"
     gene            complement(3897..4298)
                     /gene="mscL"
                     /locus_tag="PKK36_06175"
     CDS             complement(3897..4298)
                     /gene="mscL"
                     /locus_tag="PKK36_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013981848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNR94182.1"
                     /translation="MSFAKEFKEFAMRGNVMDMAVGIVIGGAFGKIVSSFVADVIMPA
                     IGMLTGGVDFSKLKITLKAASEGVEAVTINYGMFINTIIDFIIIAFAIFLVVKAMNAA
                     KKKEAAAPAAPAAPPAQEVLLTEIRDLLKKQ"
     gene            complement(4331..5044)
                     /locus_tag="PKK36_06180"
     CDS             complement(4331..5044)
                     /locus_tag="PKK36_06180"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNR94183.1"
                     /translation="MRKLITGLLAIGLAAAAGAAEEKELGWKNSAGIGLSMTDGNSET
                     LVANASAKSEHTAEAGILTLGIEGNYGEADDEKNVENAKAMANYKRTYDAFYAYLDGN
                     VLYDDIAAIDYRATVGPGLGKFLVDTEKTKLGVEAGVACIFEDVADVTEDYAALRLAQ
                     NFSRQISETAKVFESVEYLPEIEDFDNYLLNGEVGIEAAINSSASIRVSVKDKYDSTP
                     GEGLEENDVSLIAALNFIL"
     gene            complement(5311..5979)
                     /locus_tag="PKK36_06185"
     CDS             complement(5311..5979)
                     /locus_tag="PKK36_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94184.1"
                     /translation="MSRDRQFDFWYAVNNTHVLILPKSRLETFGTSMVNYHLLTELMD
                     SPKVRVREGRIEAYRPEIITPDNFARTALEGFGGPEASLYLEWLREHEKELMILKYGF
                     AIRKQEINDHIVSETLPVVIDQVREALAEKDDPLSAMVVGVDEPWEVCLIKLMQEVVQ
                     QAAPQHALDLRRDPKGYRHEIEKDFSDASRDRSKISALGAKLQKLSLFEEYEDRFYAL
                     VRAR"
     gene            complement(5976..8678)
                     /gene="acnA"
                     /locus_tag="PKK36_06190"
     CDS             complement(5976..8678)
                     /gene="acnA"
                     /locus_tag="PKK36_06190"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNR94185.1"
                     /translation="MKQFGFCLKELDIGGGRRAAYYSLAALEAQGLGGISRMPFSVKI
                     LLESLVRMQGHPAYQPEQVLSLCRWNKHADPRTEYPFLPSRVLLQDFTGVPCVVDLAA
                     LRSALARTGQNPGKIEPLIPVDLVIDHSVQIDCAGSAASFECNLKKEFERNRERYVFL
                     RWGQDAFRKLLVLPPGLGICHQVNMEYLATCVNLLPDGEGRLTASPDTLVGTDSHTVM
                     INSMGVLGWGVGGIEAEAAMLGQPIPILSPSVLGFRLTGRPGENVASTDIALTITQML
                     RKKGVVGSFVEYFGEGLDHLSLPERAPIANMAPEYGATMGFFPIDGATLDYLRQTGRS
                     EDQVRLVEAYCREQGLFRTPGAEEPEFTDVIELDLSSVEPCMAGPKRPHDRIAIQNIG
                     REFSASLAKPAGKDGFGVPADETGRKVPVEGMNGELEHGSVVIAAITSCTNTAGPALM
                     LGAGLMAKKAVKFGLKVPPQVKASLAPGSQAVTGYLKAAGLLEPLEKLGFFVDAYGCG
                     PCIGNSGPLAPAIEKAVRENDLVAAAVLSGNRNFEGRVHPLTKANYLGSPLLVLAYAL
                     KGTVKGDIQNEPVGKSRDGRDIYLKDLWPSREELEAVLPHADRPDLYKEAYGDIAGCN
                     PMWNELPAPSGTCFEWDAGSTYIQEPPFFKGFAETAPGAQDIPAARVLGFFGDFITTD
                     HISPAGSIPAGSPAEKFLLSKNISRDMFNSFGSRRGNHEVMMRGTFGNIRIRNRLASR
                     EGGFTRHLPDGEEMSFYDAAMRYLAEDQSLVVLAGKMYGAGSSRDWAAKGTALLGVKA
                     VIAESYERIHRSNLVEMGVLPLQFIEGQNADSLGLTGRELFRIGGIAEGLVPGKLLTV
                     NAAADNGKTVMFKALCRIDAPIEVEYFRHGGILQYVLRNMMKNQ"
     gene            8890..9693
                     /locus_tag="PKK36_06195"
     CDS             8890..9693
                     /locus_tag="PKK36_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94186.1"
                     /translation="MKKLMVYMTVALLAGSVAAEPQRPLLTLENKFPEQGQVEVGTLL
                     SLVEIVDNNEFDRSGDRDERTYEAYLRYGLTENLALVAGIPYMEIEPDLGDTESGISD
                     ATLGVQLKVFEDIFSYPWIIPHAQIRFDTGDEDEGLGAGEMGYEVGVSMGTTVSPSWD
                     WIDGAGRWGFKNVYHFIADASYEINENADNIAMFSGTIIWEASDKLALLAEAAITDEE
                     LEPDDGHPSYFQAGFAYQATKNFALNFYGGGAKNTDEDVRITLKASYTF"
     gene            9759..10853
                     /locus_tag="PKK36_06200"
     CDS             9759..10853
                     /locus_tag="PKK36_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94187.1"
                     /translation="MVSCTAVWGQFTRPASPRAKPRIVQEVPHPEEQIRKYSALLTLE
                     SADAAARAQMFHDRGVLYAATGNDAAALADFGAALELNPQNVMARCNRAHLLARQGRT
                     EEALAEYARVLAADPLMAEALYSRGVLFQEQGLLEEALHEYLSAFAADRRHADAAWRA
                     AQLQKEAGRREDALKIFRELADTFPDRPEFHFECGLLLEELGRPDEALRFYNTAAIRN
                     PEAPAAFFNRAGLHYRAGRTDEAAADYSRILELDASLAMAWVGRGQALARMHDYAGAV
                     RDFSRALELDQELAPAWLQRGRANYARHDYRQCIVDLTEYMRRAGRDPDVLMLRADAL
                     DAFGKPVRAAEDRQRARALKAQSPPPAQSP"
     gene            complement(10816..11715)
                     /locus_tag="PKK36_06205"
     CDS             complement(10816..11715)
                     /locus_tag="PKK36_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNR94188.1"
                     /translation="MIYFLKISRAPFLSAVLVPVLVGVAAAARDAGGVSPAKTALVVL
                     TAALIHLGANLLNDWFDYKLGADKINPHRTPFSGGSADIAEEKTCPEILLPYAALCFG
                     LAGAAGATLMVLAGDQWPVLAAIGAAGALLGISYTCPPLKLSWRGLGETAVFIAFGVL
                     PVMASMQVLAGGMSRLAWHAAFPMGLLVAAIIWINEFPDHDSDKQAGKNTLVVKLGLS
                     RARYVYHLLMFSAFASLVFLRMGGMVSGWIWLALPALFTAAAASVILHRSYDRPARLV
                     PAQALTIGTHLLTGIALAAGIAL"
     gene            11787..12872
                     /locus_tag="PKK36_06210"
     CDS             11787..12872
                     /locus_tag="PKK36_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR94189.1"
                     /translation="MSDIRCPLKEAAHISMNQEAVIGKDGALLYSEYEQCVDAAAQLL
                     RRRGCRPGDGVALAVDPDWRLPILVLAVLRAGGRVCPLQAGIPSAHLQSLMDAADCSF
                     VVSEMTAPAGFSGTVLRPEDLTGYTAPFVKGAFPLMPLGRPASVYFDLDAEGRRRALL
                     FSYGNHYYSARSANDRMSLRSGDRILLSTPPWRVESLNTIFRAVLSGAALVVPGDMTD
                     VGSAVQKYGVTHLVLTPVQYGKLSAGFAEGMFPSLKSILICGEPEPEVKTPAVRGLRV
                     FSSFILPEMSSFVSAGETGRPALPLRFGEVKISADGKIMVRGETLFMGYLDGKHVVCP
                     VDADGWFETACRGTLDLNGGLAAGDMA"
     gene            12869..>14158
                     /locus_tag="PKK36_06215"
     CDS             12869..>14158
                     /locus_tag="PKK36_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR94190.1"
                     /translation="MKKLWLSFVVILWAASMIWLIRYEAFPHLFTHRIGGYRNIFASA
                     PVLMDSWMKILFDGRHIGYSHTLVDVDDQGEPQQYVVQNETRMNLNIMGRLQRVTIDV
                     TARLDMTYLLREFTFDMRAEQHAVQLAGRRREDGLFDIEIESPAGMQRTQMEIPDDVV
                     IHSALLEVGLSSMEPGDVMHVRTLDPATLSLMDMEVRALRREEVKGEPALVLASTVQG
                     IEFMSWVDDKGNLLRQETPFGWVLEAAEDTEATAFERDSSGQADMLRRLAIPSATMLR
                     DARSVRRLKVRLDGLKLSPDELVTSRQRVVSSGPEGAVLEISRVPLSDDAVPDPARYL
                     SATPFVQSDHRDILVRAGQITGGLESKQEMARAICDWVYGYLEKNPSASLPSAIEVLR
                     VGEGDCNEHTYLFVALARAAGIPARIHLGVVYLDGAFY"
BASE COUNT         2778 a         4174 c         4316 g         2890 t
ORIGIN      
        1 tgatgaatcc ggcaaccggc agcccaaatg gctttgctgt ttccatcttc agcgccccat
       61 ctgacgactc tccgcgggac tatctcggga gtcttgctgg atccgcagcc cccgtggccg
      121 gaggactcta tacgtacacg gcctcaggca ttaccattcc aggtacgggt ttctatttcg
      181 ttctcgttac cagctcaacg cccaccaatc agggcgcgtt caactggagc gccagcgaca
      241 gcttcactca taacggaagc tggtacatca acagtgcgta ctacacgtct tcagacggtt
      301 tgaattggca aaagcacatt cgcgacgaag tctttcagat ggcgatccat gcgaccattg
      361 tgcctgagcc ggcaacgggc tggctgctgg gtgtggggct gctttcgtta agcctctgcc
      421 gtcgtcgact ggcgcccgaa ttttgcattt tccatagagc ttgacctgcg ggcggatgcc
      481 ccgtacactg cccgcccaaa tgtttttcag tttctttgac tgagtgagga gtatctcgat
      541 tatgcagaaa aatgctgcga agaaaaaatc cggaaaagat cagccggcgg atgtgaagga
      601 gcggctggcg aactttgccc ttctttttga actcgataat ctggccgtta atgtgcggga
      661 gctcagtttt gaaattctca aaacgctgct cgcaaagaac gactgcaagc tcgcggaggt
      721 ccatttctgc cggtacggac tggccgccgc cccggaaaat tatcttcccg cgctgcttca
      781 ggctctggat gtcaaaaaac cctcggccga gaagctggcc aaggatctgt acaaggcgct
      841 gggcgagcag cttgcgggcg ggaagacggt tctgaatgcc gcgctggacg ccgtgctggc
      901 cgagaccgcc ggactgggaa ttcgcatggc cgcgatcagc gcgctgcctg aagatatggc
      961 ccatgcgctg gccgaaaagc tgggcctcgc cgaccgcggc gtggagctgt ttgtcttcag
     1021 cgagccggag gatgtcttcc cgcgcgccga cacctggctc aaggtggcca agtccatgtc
     1081 ggttccgccg cgccactgca aggtgctctc gacgagcatg gcctcctgca aggcggcgct
     1141 ctccaccgga atgaacagcg tggccgtgcc cgaccggttc acctccttcc atgacttcgg
     1201 cggcgccact atggttcttg atgctctggg ggatgtttcc gcgacggatc tcgtggccga
     1261 aatctttccg ctggaagatc tgtgaccgga tggaggagcg ccccatagac cggcttctgg
     1321 gcatcatgcg gcgcctgcgg gccgacgacg gctgcccgtg ggaccgcaag cagaccgtgg
     1381 attccctcaa gacctccgtg ctcgaagaag cctatgaggt tgtggatgcc attgagtccg
     1441 gccgccgcga gccgctgtgc gaagagctcg gcgacctgct gatgctgatt gttttcctct
     1501 cccagatctg cgacgaagaa aaggcgttca cctttgaaga cgtggccacc ggcatttgcg
     1561 agaagctggt tcgccgccat ccgcatatct tcggcgatgt gaaggtcgcg aatgccgacg
     1621 aagtcatccg gaactggaac gccatcaagc agaccgagaa ggcggagaaa aaatccatca
     1681 tcggaaacat cccgcgccat ctgccggcgc ttcaaaaggc gcatcacgtg cagaagcgcg
     1741 ccgcgcaggc cggcttcgac tgggacgaaa tccgcgacgt ggtcgccaag ctggatgagg
     1801 aactggccga ggtgaaggag gccatggcct cacagaacgg aaagaaaatc gccgaggagc
     1861 ttggggatct tctgttcagc gtggtgaatg tcagccggtt cctgggtcac gatgccgagg
     1921 cggccctgaa cgccacggtg gataaattca tccgccggtt ccatgccgtg gagaagggcg
     1981 tgcaggagca ggggcgggag atgaccgact gcacgctcgc cgaactggat gttttctggg
     2041 acgccgccaa ggctcaggaa ggcaccgact gattcttctt ccggagatat tcgtcaaact
     2101 cctccggcgc gtgaaacagc tccacataag gcgtgccctg catttcacag acctcgggcg
     2161 gattgccgtg gctccggcag atttccggcc gccggtcata gatgcggcac tggttgtccg
     2221 cggtcagctc ctcgcacggc gtaacaaatt ccacaaacca tgcgccgtcg tgatcaatga
     2281 aaacctgcac gtcccggtgc agcagatacc agcggatatg gtcaaactcc tccttcgcgg
     2341 agggcgcctg gatttcgatg gccacgtagc ggcagcacag ggcgggacac ttcccgcaga
     2401 gcggatttga cgcactctca gactgattcg ctttttccat gaggtgcgtt tgtacccgct
     2461 gtgccccggg ccgtccactt ttttgtgtgc gatgtggaat agttttattg atgattggag
     2521 gggggattca taggatatgc gcgtcatgaa gaacaaagaa gtcgaacggc aaatcagcat
     2581 tctggcggac cgcacggtgg atctgcattc cgtgcatgag ctgcgcggca aactcgagcg
     2641 ctcgctggcg gaaaaccgtc cgctccgggt caagcttggc gcggacccct ccgccccgga
     2701 tatccacctg ggtcatgtgg tgccgctgaa caagctgcgc gagttccagg acctgggaca
     2761 tcttgtcgtt ttcatcatcg gcgatttcac ggggatgatc ggcgacccct ccggcaagtc
     2821 ggccacgcgc ccgcagctca cgcgcgagca ggtgatggcc aacgcgaagt cataccagga
     2881 gcaggtcttc aaggtgctgg atccggaaaa aacggaaatc cgcttcaact ccgaatggca
     2941 tgctccgatg acgttcgagg atgtcatccg gctttccgca cacgtcaccg tggcgcagat
     3001 tctcgcgcgt gatgattttg cgaagcgcta tgccgccagc cagccgatct cgctggtgga
     3061 attcctctat ccgctggtgc aggcctatga ctctgtgatg atcagggccg acgtggaact
     3121 gggcggcacc gaccagcttt tcaacctgct cctgggccgt gaactgcaga agacctacag
     3181 tcaggagccg cagattgtga tgaccctgcc gctcatcgag gggctggacg gcgtcaacaa
     3241 gatgagcaag agccttggca actacatcgg cgtgaacgag cccgcgcggg aaatctacgg
     3301 caaggccatg tccgtgagcg atgcgctgat gtggcgctat ttttcgctga ttctctgcat
     3361 gccgcaggat gaaatcgccg aaatcaaaaa agcggttgag accggcgcgc ggcatccgcg
     3421 cgatgtgaaa gatgagctgg cccggaaact ggtggccaaa ttccacgacg aggctgcggc
     3481 tgcgcaggcc tcggacgagt tcaagcgcat cttctcgcag aatcaactgc cggatgacgt
     3541 tccggaggtc gcgatggcgg gtgaaaaaag cggaattgtg gatctgctcg tgaacgcgaa
     3601 gctggccccc agcaggggcg aggcccgccg cctgatcgag cagggcggcg tgaaaattga
     3661 cgacgagaag gtctccgaca tccacgctga aatcccgctc aaaaccggca tgatcatccg
     3721 cgccggcaag cgcaaattcg caaagattgt tttgtcttga acgggtaggg cgggcactcc
     3781 gtgcccgccg cggcggtcgg cggagcgacc gccctaccag ggactgcatt agagtcggac
     3841 tgtgaaaaaa agggacgcgg cgaaccgcgt ccctttctat gcagactgcc tgaaaattat
     3901 tgcttcttca gcagatcgcg gatctcggtc agaagcactt cctgcgcggg cggggcggcg
     3961 ggcgccgcgg gcgcggccgc ttcctttttc ttcgccgcgt tcatggcctt gacgacgagg
     4021 aagatcgcga aggcgatgat gatgaaatca ataatggtgt tgatgaacat gccgtagttg
     4081 atggttacgg cctcaacgcc ctcggaggcg gccttcagcg tgatcttgag tttcgaaaaa
     4141 tccacgccgc cggtcagcat tccgatcgcg ggcatgatga cgtcggcgac aaacgagctg
     4201 acgattttcc cgaatgcgcc gccaatgaca atgccgacgg ccatgtccat cacattgccg
     4261 cgcatcgcaa actctttgaa ttcctttgca aatgacattt cttttttctc ctgtttgtat
     4321 ttcgtgttgt ttacagaatg aagttcagcg cggcgatcag ggagacatcg ttctcctcga
     4381 ggccttcgcc cggggtgctg tcgtacttgt ccttcacgga cacgcggatg ctggcgctgg
     4441 agttgatggc ggcttcgatg ccgacttcgc cgttcagcag gtagttgtca aaatcctcga
     4501 tctcgggaag gtattccacc gattcaaaga ctttggcggt ctcgctgatc tggcgcgaga
     4561 agttctgggc aaggcggaga gcggcgtagt cttccgtgac atccgcaaca tcctcgaaga
     4621 tgcaggccac gccggcttca acgccgagct tggttttttc ggtgtccaca aggaactttc
     4681 cgaggccggg cccgacggtt gcgcggtagt caatggcggc gatgtcgtca tagaggacat
     4741 tgccgtccag ataggcgtag aacgcatcgt aggtgcgctt gtagttagcc atcgccttgg
     4801 cgttttcgac gttcttctcg tcgtcggctt cgccgtagtt gccctcgatc cccagcgtga
     4861 ggatgccggc ctcggcggtg tgttcggatt tcgccgatgc attggccacc agcgtttcgc
     4921 tgtttccatc ggtcatggaa aggccgattc cggcgctgtt cttccagccc agttctttct
     4981 cttcggccgc tcccgccgcg gcggccagtc caattgcaag cagtcctgtt atcagtttgc
     5041 gcatgatatg ctcccttttg tgcgattgtg tttaaaaaag accgatcgcc cccgggcgat
     5101 cagctcaggc agataacata gcagaaaatc agaatttgtt tagcaaaaat caagaaaccg
     5161 agtgaaaaac cggaaaaaac accaaaaaat gacgaaaaaa cgccaaaaaa tgctaaaaat
     5221 cgtcaaaaat cgccgtaaaa acgcaaaaaa cggtggaaaa tgaccgaatt tgcttgaaaa
     5281 ttcagaaaaa ttgtgctaaa aacaaaaaag tcagcgcgcg cggacgaggg catagaaccg
     5341 gtcctcgtat tcctcaaaaa ggctcagttt ctggagtttt gcgcctaaag cagaaatttt
     5401 tgagcggtcc cgtgaggcat ccgaaaaatc cttttcgatt tcgtggcgat atcccttcgg
     5461 atcgcggcga aggtccaatg catgctgcgg cgccgcctgc tgcaccactt cctgcatcag
     5521 cttgatcagg cagacttccc atggttcatc cacgccgacg accatggcgc tgaggggatc
     5581 gtccttttcg gcgagcgctt cgcggacctg atcaatgacc acgggcaggg tttccgagac
     5641 gatatggtcg ttgatttcct gtttccggat cgcaaaaccg tatttgagga tcatgagctc
     5701 cttttcatgt tcgcggagcc attccagata cagggaggcc tcgggtccgc cgaagccctc
     5761 aagcgcggtc ctggcaaaat tgtccggggt gatgatttcc ggccggtagg cttcgatgcg
     5821 gccctcgcgc acgcgcactt tcggcgagtc catgagttcg gtgaggaggt gatagttcac
     5881 catcgaggtc ccaaaagttt cgaggcggct tttgggcaga atgagcacat gcgtgttgtt
     5941 caccgcatac cagaaatcaa actgccggtc tctgctcatt gattcttcat catgttccgc
     6001 aatacatact gcaatatgcc gccatgccgg aaatattcaa cctcgatggg cgcgtcaatc
     6061 cggcagagcg ccttgaacat gactgttttc ccgttgtcag cagcagcatt gaccgtcagt
     6121 aattttccgg ggacgaggcc ctcggcgatt cccccgatgc ggaacagctc gcgaccggtg
     6181 agccccagcg agtcggcatt ctgtccttcg atgaactgca gcgggagcac gcccatttcg
     6241 accaggttgc tgcggtggat gcgttcatag ctctcggcga tgacggcttt gacgcccaga
     6301 agagccgtgc ccttggccgc ccagtcgcgc gaggagcccg cgccgtacat tttccccgcc
     6361 agcacaacga ggctttgatc ctcggcaaga tagcgcatcg ccgcatcata gaagctcatc
     6421 tcctcgccgt ccggcaggtg gcgcgtaaac ccgccttcgc gcgaggccag acggttgcga
     6481 atgcggatgt tcccgaaggt ccctcgcatc atgacttcat gattgccccg gcgcgacccg
     6541 aaggagttga acatatcccg cgaaatgttt ttcgagagga gaaatttctc cgccggactt
     6601 ccggccggaa tgctccctgc cggcgagatg tgatccgttg tgatgaaatc gccgaagaag
     6661 cccagcaccc gggcggccgg gatatcctgt gctccgggcg cggtctctgc gaagcccttg
     6721 aagaagggcg gttcctgaat gtaggtggaa ccggcatccc attcgaagca cgtgccggat
     6781 ggcgccggca gctcgttcca catgggattg cagccggcga tgtcgccata cgcctctttg
     6841 tagagatccg gccggtccgc atgcggaagc acggcttcga gttcctctct cgacggccaa
     6901 aggtctttca gatagatatc ccgcccgtct cggcttttcc cgaccggttc gttttgaatg
     6961 tcacccttca cagtgccttt gagcgcatag gccaggacca gaagcggcga gccgagatag
     7021 ttggctttgg tcagcggatg cacgcggcct tcaaaattcc ggttcccgct gaggaccgcg
     7081 gccgccacaa gatcattttc ccggacggcc ttttcgatgg cgggcgccag ggggccgctg
     7141 ttgccgatgc acggtccgca gccgtacgcg tccacgaaga acccgagctt ctccagcggt
     7201 tcaagcagtc cggcggcctt cagatagccg gtgacggcct gtgatcccgg cgccaggctg
     7261 gccttgacct gcggcggcac cttcagtccg aacttgaccg ctttttttgc catcagtccc
     7321 gcgccgagca tcagcgcggg cccggctgtg tttgtgcagc tggttatcgc ggcgatcacc
     7381 acgctgccgt gttcaagctc tccgttcatg ccttccaccg gaactttcct gccggtctca
     7441 tcggcgggca ctccaaatcc gtccttgccg gcgggtttgg cgaggctggc tgaaaattcg
     7501 cggccgatgt tctgtatggc aatgcggtca tgcggccgct tcggcccggc catgcagggc
     7561 tcgaccgacg acaggtcgag ctcgatcaca tcggtgaatt ccggctcttc cgcgccgggg
     7621 gttctgaaca ggccctgttc cctgcaatag gcttcgacaa gcctgacctg atcttcggac
     7681 cgcccggtct ggcgcagata gtccagcgtg gcgccgtcaa tcgggaagaa tcccatggtg
     7741 gcgccgtatt cgggcgccat gttggcgatg ggcgcgcgct ccggaagcga aagatgatca
     7801 agcccctcgc cgaaatattc cacgaagctt ccaaccacac cttttttgcg gagcatctgc
     7861 gtgatggtca gcgcgatgtc ggtggatgcg acattttctc ccggccggcc ggtcagcctg
     7921 aaccccagca cgctgggcga aaggatcggg atcggctggc ccagcatcgc ggcttccgcc
     7981 tcgatgccgc ccacgcccca gcccagcacg cccatgctgt tgatcatcac cgtgtgcgaa
     8041 tccgtgccca ccagggtgtc gggacttgcg gtcaggcggc cttcgccgtc cggcagcagg
     8101 ttcacgcagg tcgcaagata ttccatgttc acctgatggc agatgccgag tcccggcggc
     8161 agcacgagca gtttgcggaa cgcatcctgg ccccagcgaa gaaacacata gcgctcgcgg
     8221 tttcgctcga attccttctt gaggttgcat tcaaatgagg ccgcggaacc ggcgcagtca
     8281 atctgcacgg aatgatcaat cacaaggtcc accggaatca gcggttcgat ttttccaggg
     8341 ttctgtccgg tccgggccag cgcgctgcgc agcgcggcca gatccacgac acagggcacc
     8401 ccggtgaagt cctgcagaag cacgcgcgag gggaggaacg gatattccgt gcgcgggtcg
     8461 gcgtgtttgt tccagcggca gaggctcagc acctgctccg gctgatacgc cgggtggccc
     8521 tgcatgcgca caagactctc gagcagaatc ttgaccgaaa acggcatgcg cgagatgccg
     8581 cccagcccct gcgcctccag tgccgcaagg ctgtaatagg ccgcccgccg gcctccgccg
     8641 atatccagtt ccttcaggca aaatccaaac tgtttcatat tcttcatcct tgcagctgca
     8701 cggcccgccc gcgggcgtga aaggcggaac gttaggattt tatgtttcgg atgtcaatgg
     8761 atgccggccc cgcccgccga aagatggtcc gtcacccgaa tttttatgtt ggctctttcg
     8821 ccgcgggtcg gttaaagtgc cccccagcat tgggtcttgt ttgttttcag cgaaggagaa
     8881 tacgacagaa tgaaaaaatt aatggtgtat atgacggttg cgcttctggc gggatcggtt
     8941 gcggctgaac ctcagcgtcc tcttttaacg ctggaaaaca aattccccga acagggacag
     9001 gttgaggtcg gcaccctgct gtcgctggtt gaaattgtgg acaacaatga gttcgaccgt
     9061 tcgggtgacc gcgacgagcg cacctatgag gcctatctgc gctacggact gactgaaaac
     9121 ctggcgctgg tggccggcat tccctatatg gaaatcgagc ccgacctggg ggacaccgaa
     9181 agcggaatca gcgatgccac cctcggtgtc cagctgaaag tctttgaaga catcttctcc
     9241 tatccgtgga tcattcccca cgcgcagatc cgttttgaca ccggcgacga ggacgagggg
     9301 ctgggcgccg gcgaaatggg ttatgaggtt ggcgtttcca tgggaaccac cgtgagcccg
     9361 tcctgggact ggattgacgg cgccggccgc tggggcttca agaatgtcta tcacttcatc
     9421 gcggatgcca gctatgaaat caatgagaac gcggacaaca ttgccatgtt ctccggaaca
     9481 atcatctggg aggccagcga caaacttgcg ctgctggccg aagccgccat caccgatgag
     9541 gaacttgagc ccgatgacgg gcatccgtcc tatttccagg ccggcttcgc ctatcaggca
     9601 accaaaaact tcgccctgaa cttctatggc ggcggcgcca agaacacgga cgaggatgtt
     9661 cgcatcacgc tgaaggcctc ctataccttc tgaagatgac agggcacagc gtcctgtttg
     9721 acaaaacagc acccctgaaa gcggggtgct gttttgtttt ggtttcctgc acggccgtct
     9781 ggggccagtt cacccggccg gcgagtcccc gggcgaaacc ccgcattgtg caggaggtgc
     9841 cgcatcccga agagcaaatc agaaaatatt ccgcgctgct gacgctggaa tccgcggacg
     9901 cggcggcccg cgcgcagatg tttcatgacc ggggagtgct ctatgccgcg accggcaatg
     9961 acgccgccgc gctggcggat ttcggcgcgg ccctggagct gaacccgcag aacgtgatgg
    10021 cgcgctgcaa ccgggctcat ctgctcgccc ggcagggaag aacagaggag gcgctggcgg
    10081 aatatgcccg ggtgctggcg gccgatccgc taatggccga agccctgtat tcccggggtg
    10141 ttctttttca ggagcagggc ctgctggagg aggccctgca cgagtatctg tccgcctttg
    10201 ccgcggaccg ccggcatgcg gatgccgcct ggcgcgccgc gcagcttcag aaagaggcgg
    10261 gccgcaggga ggacgccctg aaaatcttcc gcgagcttgc ggacacattc ccggaccggc
    10321 ccgaattcca ttttgaatgc ggcctgcttc tggaggagct gggccgtccg gatgaggcgc
    10381 tccggttcta caacacggcg gccatcagaa atccggaagc cccggcggcc tttttcaacc
    10441 gcgcgggcct tcattaccgc gccggccgca cggacgaggc cgccgccgat tacagccgca
    10501 ttctggagct tgacgcgtcg cttgcgatgg cgtgggtcgg ccgcgggcag gcgctcgccc
    10561 ggatgcatga ttacgccggg gccgtgcgcg atttttcacg cgcccttgaa cttgatcagg
    10621 aactggcgcc ggcatggctt cagcgcggcc gcgcgaatta cgcacggcac gattacaggc
    10681 agtgcatcgt ggatctgaca gagtacatgc gccgggccgg gcgcgatccg gatgtcctga
    10741 tgctgcgggc ggatgcgctg gatgcctttg ggaaacccgt ccgcgcggcg gaagaccgcc
    10801 agcgggcgcg cgccctcaaa gcgcaatccc cgccgccagc gcaatccccg tgagcagatg
    10861 ggtcccgatt gtgagcgcct gcgccggaac cagccgcgcg ggccggtcat aactccggtg
    10921 cagaatcacg gaagccgccg cggccgtgaa cagggcggga agcgcaagcc atatccatcc
    10981 gctgaccatt ccgcccatcc gcagaaacac cagggatgca aaggcggaaa acatcagaag
    11041 atggtagaca tagcgcgcgc gggagagccc cagtttgaca accagcgtgt ttttccccgc
    11101 ctgtttgtct gaatcgtgat ccggaaattc gttgatccag ataatggccg cgaccagcaa
    11161 acccatgggg aaagcggcat gccacgcgag ccgcgacatg cctcccgcaa gcacctgcat
    11221 ggaagccatg acgggaagaa cgccgaaggc gatgaataca gcagtttccc ctaggccgcg
    11281 ccaggacagt ttgagcggcg gacaggtgta gctgatgccc agcagggcgc cggccgcccc
    11341 gatggctgcc agcacgggcc attgatctcc ggccagcacc atcagcgtgg ctccggcggc
    11401 tcccgcgagg ccgaagcaga gtgcggcgta gggcaggagg atttccggac aggtcttctc
    11461 ttccgcgatg tccgccgacc ccccgctgaa cggagtgcga tgcggattga ttttgtcagc
    11521 tcccagtttg tagtcgaacc agtcattcag gagattggct cccagatgga tcagggcggc
    11581 ggtcagcacc acaagggccg ttttcgcggg gctcacgccg cccgcgtcgc gcgcggccgc
    11641 ggcgaccccg accagaaccg gaaccaggac cgcgctcaga aaaggagccc ggctgatttt
    11701 caggaaatat atcatttcgt tctgtcccga tgtttgtttc gaagtatatt gatcacaact
    11761 tgtttggcaa cctgtgggat gcataaatgt cggatatccg ttgtccatta aaagaagctg
    11821 ctcacatctc gatgaaccag gaggcggtca tcgggaagga cggagccctg ctgtacagcg
    11881 aatacgagca gtgcgtggat gccgcagccc agctgctgcg gcgccgcggc tgccgcccgg
    11941 gtgacggcgt ggcgctggcg gtggatccgg actggcggct cccgattctt gtccttgccg
    12001 tgctgagggc gggcggccgc gtttgtcctc ttcaggccgg tattccgtcc gcccacctgc
    12061 agtcgctgat ggatgcggcg gactgctctt ttgtggtctc ggagatgacc gccccggctg
    12121 gtttcagcgg aaccgttctc cggccggaag acctcaccgg atatacggct ccgttcgtga
    12181 agggagcttt tcctctcatg ccgcttggcc ggcccgccag cgtgtatttt gatctggacg
    12241 cggagggccg ccgccgcgcg ctgcttttca gttacggcaa tcactattac agcgcgcggt
    12301 ccgccaacga ccggatgtcg ctccgaagcg gagaccgcat tctgctttcc acgccgccct
    12361 ggcgcgtgga atccctgaac accatatttc gcgccgtgct cagcggcgcc gcgctcgtgg
    12421 tgcccggaga tatgacggat gtcggctcgg ccgtgcagaa atacggagtg acgcatcttg
    12481 tgctgacccc ggttcaatac gggaagcttt cggccggttt tgcggaaggc atgtttcctt
    12541 cactcaaatc catcctgatc tgcggagagc cggaaccgga ggtgaagact ccggccgtgc
    12601 gcggcctgcg cgtgttcagc agttttatcc tcccggagat gagttcgttc gtcagcgccg
    12661 gagagacggg ccggcccgcc cttcctctgc gattcggcga ggtgaaaatt tccgctgacg
    12721 gtaaaatcat ggttcgcggg gagaccctct tcatggggta tctggacgga aagcacgttg
    12781 tctgtccggt ggatgccgat ggatggtttg aaaccgcctg ccgcggaact cttgatttga
    12841 acggcgggct tgcggctgga gacatggcgt gaagaaactc tggttatcct ttgtcgttat
    12901 tctgtgggcg gcgtccatga tctggctgat ccgctatgaa gctttccctc atctgttcac
    12961 gcaccgcatc ggcggatacc ggaacatctt cgcgtccgcg ccggttctga tggattcgtg
    13021 gatgaaaatc ctgttcgacg gtcggcatat cggctacagc cacacccttg tggacgtgga
    13081 tgaccagggc gagccgcagc agtatgttgt gcagaatgaa acgcgcatga atctcaacat
    13141 catgggccgg ctccagcgcg tgacgattga tgtgacggcc cggctggaca tgacttatct
    13201 tctgcgcgag ttcaccttcg acatgcgggc ggaacaacac gccgttcagc ttgccggccg
    13261 ccggcgggag gacggattgt tcgacataga aatcgagtcg ccggcaggaa tgcagcggac
    13321 gcaaatggaa attcccgatg atgtggtgat tcattcggcg ctcctcgaag tggggctgtc
    13381 ctccatggag cccggcgatg tgatgcatgt gcgcaccctt gatcccgcca ccttgtccct
    13441 gatggacatg gaggtgcggg ccctgcgccg cgaagaagtg aaaggagagc ccgcccttgt
    13501 gcttgccagc acggttcagg ggattgaatt catgtcgtgg gtggatgata aagggaatct
    13561 gctgcggcag gaaaccccct ttggatgggt actggaggcc gcagaagata cggaggcgac
    13621 agcctttgag cgtgacagct ccggccaggc ggatatgctt cgccgccttg cgattccctc
    13681 cgcaaccatg ctgcgtgatg cgcgcagcgt ccgccgcctg aaggtccggc tggacggctt
    13741 gaagctgtcc ccggacgaac tggtcacatc acgccagcgg gtggtctcca gtgggccgga
    13801 gggcgctgtg ctggaaataa gcagggtccc gttgtcggat gatgccgtcc ccgatccggc
    13861 gcgctatctg tcggccacgc cctttgtgca gtcggatcac agggatattc tggtccgcgc
    13921 agggcagatc accggaggcc tggagtcaaa acaggaaatg gcccgtgcga tttgcgactg
    13981 ggtttacggc tatcttgaaa agaacccgtc cgcttcgctc ccgtccgcca tcgaggtact
    14041 gcgtgttgga gagggcgact gcaatgagca cacctatttg tttgtcgcgc tggcccgcgc
    14101 cgccggcatt cccgcgcgca ttcatctggg tgtggtctat ctggacgggg ctttctac
//
LOCUS       DAOQWR010000059        13958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_4853_length_13958_cov_5.511257, whole genome shotgun sequence.
ACCESSION   DAOQWR010000059 DAOQWR010000000
VERSION     DAOQWR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13958
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_13958_cov_5.511257"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2757
                     /locus_tag="PKK36_06220"
     CDS             <1..2757
                     /locus_tag="PKK36_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNR94191.1"
                     /translation="ALDLAADFLLRDWKSGPIDLRDALVIVPTRNAGRRLRERLALLA
                     AERGTAVLTGIVETPSRLFAPDKTALPVVSDVMAEAFWRKTLDAFDPRKLERLSRSGL
                     AQNPAARAAAAEHLVRLRSELCEENYDLTSFAAQTAAEKERWACLAELEAACRALLQK
                     NGWSDDVTAKLEAAAKAEVPENIKRVIVLFVPDPPPLSLRVLQKISTSLSVDVCVHAA
                     PAEKNDFDSWGRPLPGKWLRRELLLDPSQIEVCDDAEAMAELVSRHIAGLPEQARAGV
                     TVGVGDARSAVRIADELGRRGIPVFDPSDRPAARLPMFLLISRLVQVQKDLRYLSFMT
                     LARHPDALRRLETAASAPDTLLAELDDFQNRHMPSGMPDALLIAQKTGAVHVVSALEE
                     AQRWLDLLGGGPLLSESLRSVLKHVYQDVPEADECLAEAVSALHQMLEKLACTESIGI
                     SREDAADLFLRMVASAGLSPDRPEKAIELLGWLELAWEDAPVLLLTDLNDGKIPETRT
                     ADPFLPDGARRAAGLRDNAHRLARDAHILESVLRFTGRRNLRGFMPRRSPDGDPLKPS
                     RLLLQCGPDELARRAGQFFSDAGSPFASLARSPGWRIRLPALRKEIPLRHVSPSALRL
                     YLDCPFRYYLKKILGLAEPYEEQAELDALGFGTLCHDVFQCFANAPLKNSGDENKIAA
                     FLKEKTDELFERKFGANLSLPLMIQRDVIRQRMTAAAGVQARLRREGWQIAAAEQEFR
                     CAIAGVPLVGRIDRIDQNEKDGRVRVIDYKTTASAKNPEQTHITRSAKCESYKLCADG
                     KSHWSDLQLPLYVHAWRTMNPGGAENVEAAYFALPNAAGSTSLLIWENMDAGRVGDAL
                     QCAERIIRRIRAGVFWPPQPGAGPREDPLDRLFFQDIETLLDPRSREELQRRAEAFNA
                     GGES"
     gene            2754..5945
                     /locus_tag="PKK36_06225"
     CDS             2754..5945
                     /locus_tag="PKK36_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR94192.1"
                     /translation="MMRQYEAISALAGSGKTYALTSRFIGLLAQGARPEEILAVTFTR
                     KAAGEIFDRIVTRLAAAALHERARDELASALKAYGCEMKTGAPGRWLQQVLEAMPHLH
                     IGTIDSLFVSIVQAFAMELGLPPRQEILDGLGRDLAREEALRDAMEAACRDRAARQSF
                     MEAFRLLTFEKQKNVRDTILETADSAHRLFLYYPDPESWGRFDAMWPDKPWWMCAAGM
                     TRAELLAEIDAFRNGRHIAGLGNQKAAGKWNEMLDLLGREDWLELSGHSLFKNLSVRL
                     AGLLAGEAEYTNFRKYAISGSDARQVVRFLAWFMQRYVFDNLRKTEGIHRLMTAYEAS
                     YRQSVRARGRLSFEDIQVVLFEAERSGVKMEIDYRLDGRFRHWMLDEFQDTSARQWAV
                     LGNLANEILQGGDDRSFFYVGDAKQAIYGWRGGDARLFDAVHGYYENLFGRKSILNRS
                     WRSSPVVLKAVNQVFGDALSAPLHEHPEVLDRWRASWEEHQPAEKNLKLPGRVEFFQV
                     DDENDALIGKTCAVVESLHRQKGMEIAVLVRKNSFGDRVASALRSRGVAVRREVNPKL
                     CDNGVVSGLLALLDYADHPGDTFALWHLEKCGLFDALQRQWGAEAGLPARLRDEAVRS
                     GVAGLLLSVLDVCRDEDLLEEDFIEMRLRQLLAAARDFDSSGKGGPSDFAAHARSLEV
                     KDPVVSGAVTVMTIHKAKGLEFDAVVLPDLHGELGKSEAGELEPFSTVTNEDHDPSGL
                     ASGRGWIFPLPRESIAQMDETFTALRQAVMNRTMYDELCLLYVAMTRAKQGLYMIAEK
                     PPANPTVLRTDDVLRRTLADEHENPGSGGMLFEAGDTDWFRAKPAGPSAGEQKPESFR
                     PDIKVALAAGRRLMVTAPSREEGRSGQKIKVLFERGGSEGALRGTALHELFQQVEWNE
                     AGLGERILESWEPGAEALTEALRAEIRQEFLRALQSPEIITALSKPEGCAELWREQGF
                     EMADEGRWITGRMDRVVILRDADGKAKGAIVTDFKSDRVSAGTELEERAEKYGPQIRW
                     YIKAVSRLLQLPSDKIRAQLIFTCPGKVHDVHG"
     gene            5929..6204
                     /locus_tag="PKK36_06230"
     CDS             5929..6204
                     /locus_tag="PKK36_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94193.1"
                     /translation="MMSMADRELDYLEGARFCIVLMKVLDQEAGKVQLTPIYGTARVS
                     AGGLSVEESSGSIHAVPDSALASIYPSDGNEMLGDAEYYAVVKISAL"
     gene            6218..6787
                     /locus_tag="PKK36_06235"
     CDS             6218..6787
                     /locus_tag="PKK36_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR94194.1"
                     /translation="MQRDRIIQQLEAYQAKWENESQTVRRLIDFIAAHEDFHSQALRQ
                     GHITGSAWLVNRAGTHVLLTHHRKLNKWIQLGGHADGDSDPLSAALREASEESGLEKI
                     EPVSEEIFDVDIHEIAARENEPAHFHYDIRYALRAACPENYIVSDESHDLEWIKIDRL
                     QDFTVEESVLRMAAKWKRRAGKDGGRCRV"
     gene            6883..7104
                     /locus_tag="PKK36_06240"
     CDS             6883..7104
                     /locus_tag="PKK36_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR94195.1"
                     /translation="MNALNPEQLGNAIRKRRRQLKVSQKDLAMTCGTGLRFIVDLEKG
                     KPTCQIGKVLQVLQALGMKIKIEAPGMDV"
     gene            7109..8362
                     /locus_tag="PKK36_06245"
     CDS             7109..8362
                     /locus_tag="PKK36_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNR94196.1"
                     /translation="MNNLIVYLNAERVGVLEQDDTGLLRFTYDAAWTGRPGAEPLSHS
                     LPLQGDAFTGKKARAFFAGILPEEGPRKQIAKILGISDTNDFAMLEKIGGECAGAVSL
                     LPEGMSPPSSQAEHRELTDRELQDIVADLPRHPLMAGAEGLRLSLAGVQDKLPVIAHG
                     TSISLPLGGTPSTHILKPEPDRFPGLAANELFCMTLARTAGLNVPDIEYRMIGEKPCI
                     LVQRSDRKADEAGNTVRIHQEDFCQALGFPPERKYQAEGGPMLRDCISLLREWSTAPV
                     LDIPAFVGGLIFNVLIGNADAHAKNFSLLYEGGDRRLCPFYDLVSTVVWPELTTNPAM
                     KIGNCKSINVFSIAEWKKMAQECGLGWPVLREKLAEMSEAVLGHLDAVHSQTASVSED
                     FAKKLSEVMQDRAAKMLDALSRQPC"
     gene            complement(8554..8628)
                     /locus_tag="PKK36_06250"
     tRNA            complement(8554..8628)
                     /locus_tag="PKK36_06250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8594..8596),aa:Val,seq:cac)
     gene            complement(8687..10636)
                     /locus_tag="PKK36_06255"
     CDS             complement(8687..10636)
                     /locus_tag="PKK36_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94197.1"
                     /translation="MSRGDKAHFALGLAGCALLMLLLACTPGISFTEDLGRHLLLGKI
                     ILAERAVPQTNLLTYTHPEFPFINHHWLSEIFLYLAHRAAGLNGLIAWKMIMMTLGLL
                     CAMLARKPRGEFNLYWLAGIFSAVILGFRAHIRPELFTYLNVALLMLLLNQVRKGKRW
                     PRFVIPPLMLLWANAHIYFIFGLGMLFLFLIERLIGSEPQKRVRETGWFALAVLFSAA
                     NPNFLKGLLYPFAIFRNYGVAITENASPLEYWHTVLNPMLIALPLLSLLFFIALGRLI
                     ITRFRTRDTSSEGLAEIFTALAAFAAACLMARSAPLFALTALPVIAAAPSRTRRWAAA
                     PLLILNILLCAAVVEGKYARIFPSPIGPTPFGFDDESRYQRLTALARDYGLKGPVFTD
                     YNIGSLVEYQLWPEPGYVDNRPEAFPAAFWRTEYEPALALGPAWEEISARRGFNAIIV
                     SLTGVKEAFCRELMRRPEWVLVHLDEFCGMWVRNDISNKQIIDASIFNEARLGEYVQD
                     LGRRIGQLPEISAWRRQIEADVIVYRLYSLICVDATPAVWPMLKKVHLMYPDWQIVHE
                     LMRVSVPPEDIQMAEQVLAEKARWPLAAKQVLDWGHVLLARKEIAEAQRVFRRGRFFF
                     PLSPALREALRICDDLEYVTAPKNN"
     gene            complement(10639..12579)
                     /locus_tag="PKK36_06260"
     CDS             complement(10639..12579)
                     /locus_tag="PKK36_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNR94198.1"
                     /translation="MDFFDLQENARKKTRILVFYFLLTIAALIGSLYYVFALLVRLNS
                     KPPQPLWIPELFLGVTLGVLLLVLLGSLYKIAALRGGGAAVAQSLGGSLVPPNTRDFK
                     ERRLLNVVEEMAIAAGMPPPPVYVQRNEKGINAFAAGYSQDSAIVCVTQGCLDALSRE
                     ELQGVIGHEFSHILSGDMRLNIRLIGLLHGILLISILGRIILRSMGRSSGNRKGGGGI
                     ALVALAMLVFGSIGVFFGRLIKSAVARQREYLADASAVQFTRDTTGIARALKKIGGFS
                     LGSRIGHAAAEEASHMFFSNGFRQSLFNMMATHPPLEERIRQLDPSWDGQFIAAEKWQ
                     AESGAGTAEPEKKSALGALPKIPGLPEVLNAPALGAMAISAEALGKTVGTPRPENVEA
                     VQAVLARVPDEVLNAARDPFGAQALIFALLLDEDKPEPLAKQLDWLKSTTDAAVREQT
                     DQFRPAVAALPPESEPALIDICRNSLLSLSEKQFRHFLNTVDQLIAADGELDLFEYMI
                     QHLLVRMLKDHYGPRPFRTAPVYAIRALAPGVSTILSSIAHVGSDDEAAARQAFQHAI
                     KSLSAPPDLFELLPAERCTFDSVDAAADQLTRAEPGIKKQVLAACMACLSHDRTVTRE
                     EGELFRAVAAGMGCPVPFIVPG"
     gene            complement(12616..13203)
                     /locus_tag="PKK36_06265"
     CDS             complement(12616..13203)
                     /locus_tag="PKK36_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNR94199.1"
                     /translation="MSTGLIILAVIVVLIIIVAGMYNKLVALKNRFKNAYAQIDVQLK
                     RRYDLIPNLVEVAKGYMKHERETLEAVISARNAAASANQSVAADPSNPEAMKKLMSAE
                     NTLGGTLGRLFALSESYPDLKANQNMMQLSEELTTTENKVSFARQAYNDAVMHYNTAR
                     EVFPQNIIANMFNFGPATLFEVTVEAEKQAPKVSF"
BASE COUNT         2815 a         4082 c         4301 g         2760 t
ORIGIN      
        1 gcgctcgatc tggcggcgga ttttctgctt cgcgactgga aatccggtcc gattgatctg
       61 cgtgatgcgc ttgtgattgt gcccacgcgc aatgcgggcc gccgcctgcg cgaacgcctc
      121 gctctgctcg ctgccgaacg cggcacggct gttctgacag gtattgtgga aaccccatcg
      181 cgactttttg cgcctgataa aaccgcgctt cccgtggttt cggatgtgat ggccgaggct
      241 ttctggagga aaacgctcga tgcttttgat ccccggaaac tggaacgcct cagccgctcc
      301 ggccttgctc agaatccggc ggcccgcgcc gccgcggctg agcatctggt ccgcctgcgc
      361 tcggaactgt gcgaggagaa ttatgacctg acatcctttg ccgcgcagac cgccgcggaa
      421 aaagaacgct gggcctgtct cgcggagctg gaagcggcct gtcgcgccct gctgcagaaa
      481 aacggctgga gcgacgatgt gaccgccaag ctcgaagcgg ccgcgaaggc cgaagtgccg
      541 gaaaacataa agcgcgtgat tgtgctcttt gtgcccgatc cgcccccgct gtcgctgcgt
      601 gttctccaga aaatttccac gtcgctttct gttgatgtct gtgttcacgc cgcgcccgcc
      661 gagaagaatg atttcgattc gtggggccgc ccgcttcccg ggaagtggct gaggcgcgaa
      721 cttttgctgg atccgtcgca gatcgaggtg tgtgatgacg ccgaggcgat ggcggagctg
      781 gtttcgcgcc atatcgccgg actgcccgag caggcgcgcg ccggcgtcac ggtgggagtc
      841 ggagacgccc gcagcgccgt ccggattgcc gatgaactcg gacgccgggg catacctgtt
      901 tttgacccgt ccgatcgccc ggccgcgcgc ctcccgatgt tcctgctgat ttcccgcctg
      961 gttcaggtgc agaaggatct ccgctatcta tccttcatga cccttgcgcg gcatcccgat
     1021 gcgctgcgcc ggctggaaac tgccgcatcg gcgccggaca cgcttctcgc cgagctggat
     1081 gactttcaga accggcacat gccgtccggg atgccggatg cgctgctcat tgcacagaag
     1141 accggcgctg ttcatgtcgt gtccgcgctg gaggaggcgc agagatggct tgatctgctc
     1201 ggtggcggtc cgctcctctc cgaaagcctg cggtcggttt taaagcatgt atatcaggac
     1261 gtgccggaag ccgatgagtg ccttgcggaa gcggtttcgg cgctgcatca gatgctcgag
     1321 aaactcgcct gcaccgagtc catcggaatc agccgcgagg atgccgccga tctgttcctg
     1381 cgcatggtgg cctccgccgg cctgtctcca gaccgcccgg agaaagccat cgaacttctg
     1441 ggctggctgg aactggcgtg ggaagacgcg ccggtgctgc ttctcaccga cctgaatgac
     1501 gggaagattc ccgaaacccg gacggccgat ccctttctgc ccgacggcgc gcgccgcgcc
     1561 gccggcctgc gggacaacgc gcaccgcctc gcgcgcgatg cgcacatcct ggagtctgtg
     1621 ctgcgcttca ccggccgcag gaatctgcgc ggattcatgc cgcggcgctc gccggacggc
     1681 gatccgctca agccctcccg gcttctgctc cagtgcggcc cggacgaact ggcccggcgc
     1741 gcggggcagt tcttcagcga tgccggctct ccgttcgcat cgctggcgcg ctcgcccgga
     1801 tggcgcatcc gcctcccggc cctgcggaag gaaatccccc tccggcatgt ctctcccagc
     1861 gcgctccggc tctacttgga ctgccctttc cggtattacc tgaaaaaaat actcggcctt
     1921 gccgaaccct atgaggagca ggcggaactg gacgcgctcg gcttcggcac gctgtgccat
     1981 gatgtgtttc agtgcttcgc gaatgcgccg ctgaaaaaca gcggcgatga aaataagatc
     2041 gccgcattcc tgaaggaaaa aacagatgaa ctgttcgagc ggaagttcgg tgcgaatctc
     2101 agcctgcctt tgatgattca gcgggatgtg atccgtcagc gcatgacggc cgccgccgga
     2161 gtgcaggccc gcctccgccg cgaaggctgg cagattgcgg ccgccgagca ggaattccgt
     2221 tgtgcaattg cgggcgtccc cctcgtgggg cgcatagacc ggatagatca aaacgaaaag
     2281 gacggccgcg tgcgggtgat tgactataaa accaccgcct ccgcgaagaa tcctgaacag
     2341 acgcacatca cccgaagcgc taaatgcgaa agctataaac tctgcgccga cggcaaaagc
     2401 cactggagtg atctccagct tcctctttat gtccatgcat ggcggaccat gaatcccggc
     2461 ggcgcggaaa acgtggaggc cgcctatttc gcgctgccca atgccgcggg ttccaccagc
     2521 cttctgatct gggaaaatat ggacgccggc agggtgggcg atgcgctgca gtgcgcggaa
     2581 cgaatcattc gccgcatccg ggcgggcgtg ttctggcccc cgcagccggg cgccggcccg
     2641 cgggaagatc cgctggaccg cctgtttttc caggacatcg aaaccctgct tgatccccgc
     2701 tcgcgggagg aactgcagcg ccgcgccgag gccttcaacg ccggaggcga atcatgatgc
     2761 gccagtatga ggccatctcc gcgctcgccg gttccggaaa aacctatgcg ctcaccagcc
     2821 gcttcatagg cctgctcgct cagggcgcgc gtccggagga aattctggcg gtcaccttca
     2881 cccgcaaggc ggccggcgag atcttcgacc ggattgtcac ccgtctggcg gcggccgccc
     2941 tgcatgaaag ggcccgcgat gaactggcct ccgcgctgaa ggcgtatgga tgcgaaatga
     3001 agaccggtgc tcccggccgc tggctgcagc aggtccttga ggccatgccc catctgcaca
     3061 tcggcaccat agacagtctc tttgtcagca ttgtgcaggc ctttgccatg gaactcgggc
     3121 ttccgccgcg ccaggaaatc ctcgacggcc tcgggcgcga cctcgcgcgg gaggaggccc
     3181 tgcgcgacgc catggaggcg gcctgccgcg atcgcgctgc ccgccagtcg ttcatggagg
     3241 ccttccgcct gctgacgttc gaaaaacaga aaaacgtccg cgacaccatt ctggaaacgg
     3301 ccgactccgc ccaccgtctc ttcctgtatt atcccgaccc cgaatcatgg ggccgctttg
     3361 atgcgatgtg gccggacaag ccgtggtgga tgtgcgccgc cggcatgacc cgcgcagaac
     3421 tccttgcgga gattgacgct ttcagaaacg gcaggcatat cgcgggtctt ggaaatcaga
     3481 aggcggccgg caaatggaat gaaatgctcg atctgctcgg ccgcgaagac tggcttgaac
     3541 tgtccggcca ttcgctgttc aaaaaccttt ccgtccggct ggccggtctg cttgcggggg
     3601 aggcggaata caccaacttc agaaaatatg cgatttccgg aagcgacgcc cgccaggtgg
     3661 tgcggtttct ggcctggttc atgcagaggt atgtcttcga taatctgcgc aagaccgaag
     3721 gcatccatcg cctgatgacg gcctatgaag cctcgtaccg gcagtccgtc cgcgcgcgcg
     3781 gccggctttc ttttgaggat attcaggtcg tccttttcga agcggagcga agcggagtca
     3841 aaatggagat agactaccgc ctcgacgggc gcttccggca ctggatgctc gacgaattcc
     3901 aggataccag cgcccgtcag tgggccgtgc tcggcaatct ggccaacgag attcttcagg
     3961 gcggtgatga ccgcagcttc ttttatgtcg gcgacgccaa acaggccatc tacggctggc
     4021 gcggcggcga cgcgcgcctg ttcgatgccg tccacggtta ttatgaaaac ctcttcggca
     4081 gaaaaagcat cttgaaccgg tcctggcggt ccagtcccgt cgtgctgaag gctgtgaatc
     4141 aggttttcgg ggatgctctt tcagctccgc ttcatgagca ccccgaagtg ctggaccgct
     4201 ggcgcgcctc gtgggaggaa caccagcccg ccgaaaagaa cctgaaactg cccggaaggg
     4261 ttgagttctt ccaggtggac gatgaaaacg atgccctcat cgggaagacc tgcgccgtcg
     4321 tggaatcact gcaccggcag aaaggcatgg agatcgctgt gctggtccgg aagaacagct
     4381 tcggtgaccg ggtggcctcc gcgctgcgca gccgcggcgt cgcggtccgc cgcgaggtga
     4441 atccaaagct gtgcgacaac ggcgttgtct ctggcctgct cgcgcttcta gactatgccg
     4501 accatcccgg cgatactttc gcgctctggc atttggagaa atgcggcctc tttgatgcgc
     4561 tgcaaaggca gtggggggcg gaggccggcc ttcccgcccg cctgcgggac gaggcggtcc
     4621 ggtcaggcgt cgcagggctg cttctctcgg tgctcgatgt ctgccgcgat gaggatttgc
     4681 ttgaggagga tttcatcgaa atgcgtctgc ggcagctgct ggcggcggcc cgcgattttg
     4741 attcgagcgg gaagggcggt ccctcggact tcgccgcgca tgcgcgaagc ctcgaggtga
     4801 aggatccggt tgtcagcggg gccgtcaccg tcatgaccat tcacaaggcc aaggggctgg
     4861 aattcgatgc cgtggtgctc cccgatcttc atggcgagct tggaaaaagc gaagccggcg
     4921 agctggagcc gttcagcaca gtgacgaatg aagatcatga tccatccggc ctggcgtccg
     4981 gccgcggctg gattttccct cttcccaggg agagcatcgc gcagatggac gaaaccttca
     5041 cggccctccg tcaggcggtg atgaaccgga ccatgtatga cgaactctgc ctgctgtatg
     5101 tcgcaatgac ccgtgcaaaa cagggcttgt acatgatagc ggaaaaaccg ccagccaatc
     5161 ccacggtgct ccgcaccgat gatgtcctgc gccggactct cgcggatgag catgaaaatc
     5221 ccggttccgg cggaatgctg tttgaagcgg gcgacacaga ctggttccgc gcgaaacccg
     5281 ccggcccctc ggccggagag cagaagccgg aatctttccg gccggacatc aaagtggccc
     5341 tggcggccgg gcggagactg atggtgaccg cgccgtcgcg ggaggaaggc cggtccggac
     5401 agaaaatcaa agttctgttt gaacgcggcg gcagcgaagg cgctttgcgg ggcacggccc
     5461 ttcatgaact gtttcagcag gtcgaatgga atgaagccgg gctcggcgag cgcattctgg
     5521 agtcatggga accgggcgcg gaggccttaa cagaggcgct gcgcgcggaa atccggcagg
     5581 agtttttgcg cgccctccag tctccggaaa tcataacggc tctgtcaaaa ccggaaggct
     5641 gtgcggagct gtggcgggag cagggctttg agatggcgga cgagggccgc tggatcaccg
     5701 ggcgcatgga tcgggtggtc attctgcggg acgcagacgg gaaagctaag ggggccattg
     5761 tgacggattt caagagcgac cgcgtttcag cgggcaccga actggaggag cgcgcggaaa
     5821 aatatggacc ccagatccgg tggtatatca aggcggtttc gcgcctgctt cagcttccat
     5881 ctgataaaat ccgcgcgcag ctgatcttca cctgccccgg aaaggtgcat gatgtccatg
     5941 gctgatcgcg aactggatta tctcgaaggt gcgcgtttct gcatcgtcct gatgaaggtg
     6001 ctggatcagg aggccgggaa ggtgcagctc acgccgatat acggaacagc ccgcgtttcg
     6061 gccggcgggc tgagcgtcga ggagtcttcc ggaagcatcc atgccgtgcc cgactcggcc
     6121 ctcgcttcga tctatcccag tgatgggaac gaaatgctcg gggacgccga gtactatgcc
     6181 gtcgtgaaaa tatcggcact ttagcctcgg acgaaaaatg cagagagaca ggatcattca
     6241 gcagctggaa gcctatcagg ccaaatggga aaacgagtcg cagaccgtgc ggcgcctgat
     6301 tgatttcatt gcagcgcatg aagactttca cagccaggca ctgcggcagg gccacatcac
     6361 cggttccgcg tggctcgtca acagggccgg cacacatgtc ctgctcacac atcaccggaa
     6421 gctgaataaa tggattcagc tgggcgggca tgcggatgga gattcagacc ccctgagcgc
     6481 cgcgcttcgc gaagcctccg aagaatccgg actggagaaa atcgagcccg tgagcgaaga
     6541 aatatttgac gtggatattc atgaaattgc cgcgcgggag aacgagccgg cgcatttcca
     6601 ttacgacatc agatatgccc tgcgagccgc atgtcctgaa aattatattg tcagtgacga
     6661 gtcccacgac ctggagtgga tcaaaataga caggctgcag gatttcacgg tcgaggaatc
     6721 cgtcctccgc atggccgcaa aatggaagcg ccgggccgga aaggatggtg ggcgatgcag
     6781 ggtttgatgg ggcacgaagt ggcattgccg cattattccc gatcgggaat aattttattt
     6841 gacaaggtgg caaccgaggt ttatgttccc gatcggtaaa ttatgaatgc tctcaatcca
     6901 gaacagcttg gaaatgccat tcggaaaagg cgcaggcagt tgaaggtctc tcaaaaggat
     6961 ttggccatga cgtgcggaac gggtctgcgc ttcattgttg acctggagaa aggaaaacca
     7021 acctgccaga ttgggaaggt gctgcaggtt cttcaggctc ttggaatgaa aatcaaaatc
     7081 gaagctccag gcatggatgt ttgatttcat gaataatctg atcgtatatc tgaatgcaga
     7141 gagagtcgga gttcttgaac aggacgacac cggactgttg cggttcactt atgatgccgc
     7201 atggacgggc aggccggggg cggaacctct ttcacactca cttcctcttc agggcgatgc
     7261 attcacagga aagaaggcgc gtgcgttttt tgccggcatt ctgcccgagg aaggaccacg
     7321 aaagcagatt gcaaaaatac ttgggatcag tgacacaaat gattttgcga tgctggagaa
     7381 gattggcgga gaatgcgccg gcgccgtcag tctgctccca gaggggatga gtcctccttc
     7441 ttcacaagcg gaacacaggg aactgacaga tagagaactg caggatattg tggcagacct
     7501 cccccgtcat ccattgatgg caggtgcgga aggcctgcgg ctttcacttg ccggggttca
     7561 ggacaaactg cccgtcatcg cccatggaac atcaatctcc cttcctctgg gcggcactcc
     7621 cagcacacat atcctgaagc cggaacccga ccgttttccg ggtttagccg caaatgagct
     7681 gttttgcatg actctggcaa ggactgcggg attgaatgtg cccgatatag aatatcggat
     7741 gatcggggaa aaaccatgta ttctggttca gagatccgat cgcaaggccg atgaagcggg
     7801 taatacggtc aggattcatc aggaagactt ctgccaggca ctcggatttc ccccggaacg
     7861 caaatatcag gcggagggcg gaccgatgct gcgtgattgc atatcgcttc ttcgggaatg
     7921 gtccaccgcg cccgttcttg atatcccggc atttgttggc ggattgatct tcaatgttct
     7981 gatcggaaat gcggacgcgc atgccaagaa cttctcgctg ctgtacgaag gcggcgatcg
     8041 gaggctgtgc cctttttacg atctggtttc cacagtcgtt tggccggaac ttacaaccaa
     8101 tcccgcgatg aagattggca actgcaagtc aatcaatgtg ttttcaatcg ccgaatggaa
     8161 gaagatggca caggaatgcg gtctgggctg gccggtgctg agagaaaagc ttgccgaaat
     8221 gagtgaggcc gttttgggcc atctggatgc ggtgcattct cagacagcat ccgtgagtga
     8281 ggatttcgcg aaaaaactca gtgaagtcat gcaggaccgt gctgcaaaaa tgctcgatgc
     8341 gctttctcgg cagccctgtt gaggaatttt gcggctcctg gtaatgcggc gctcaccgaa
     8401 tgacttgacg gtagcaggcg aagcctgctg acggcaagtc acttcggtga agcggccttg
     8461 ttccgcaagc gcgctgcgcg cgccgcggac aagaccgctg aacgaagtga gcgccgcatt
     8521 cctggcgcgc gcagcgcgcc aggaataaaa ggatggtggg cgatgcaggg tttgaacctg
     8581 cgacttctac cgtgtgaagg tagcactctc ccgctgagtt aatcgcccat tccgaaagaa
     8641 tacgtgaata tgtattgcaa acccgacgga tgtcaagcca tgccgttcag ttattcttcg
     8701 gagcagtcac atattcaaga tcatcgcaga tcctgagcgc ctcgcgcagc gccggagaaa
     8761 gagggaagaa gaaacgtccc cggcgaaaaa ccctttgggc ttctgcgatt tcctttcttg
     8821 ccaaaagcac atggccccag tccaggacct gcttggccgc cagcggccat cgggcttttt
     8881 cggccagcac ctgctcggcc atctggatgt cttccggcgg cacgctgacg cgcatgagct
     8941 cgtgcacaat ctgccagtcc ggatacatca gatgcacttt tttcagcatc ggccacaccg
     9001 ccggcgtcgc gtccacgcag atcaggctgt aaagccggta cacgatgaca tcggcctcaa
     9061 tctgcctccg ccaggcggat atctccggca gttgtccgat ccgtctgccg agatcttgaa
     9121 catattcacc aagccgcgcc tcattgaaga tggaggcgtc tatgatctgt ttgtttgaaa
     9181 tgtcgtttct gacccacatt ccgcagaact catccagatg caccagcacc cattcaggcc
     9241 gccgcatcag ttcgcggcag aaggcttcct tgacccccgt gagagaaacg atgatggcgt
     9301 tgaatccgcg ccgtgcggaa atttcttccc atgctggacc cagcgcgagg gcaggttcat
     9361 attccgtccg ccagaaggcc gcgggaaacg cctcgggccg gttgtccaca tagcccggtt
     9421 cgggccagag ctgatattcc acaaggctcc cgatgttgta gtccgtgaac acagggccct
     9481 tcagtccgta atcgcgggca agtgcagtca gccgctgata gcgggattca tcgtcaaagc
     9541 cgaatggcgt gggtccgatg ggcgagggga atatccgcgc gtattttcct tcaaccaccg
     9601 ccgcgcagag caggatgttg aggatcagca gaggtgcggc cgcccaccgc cgcgtacggc
     9661 tgggtgcggc cgcgatgacc gggagcgccg tcagggcaaa cagcggcgcg ctccgcgcca
     9721 tcaggcaggc cgccgcaaag gctgcaagcg ccgtgaagat ttctgcaagc ccttcggagg
     9781 aagtgtcacg ggttctgaag cgtgtgatga tcaaacgccc cagcgcaatg aagaacagca
     9841 ggctgagcag gggcagggcg atcagcatcg ggttaaggac tgtgtgccag tattccagcg
     9901 gcgaggcatt ttccgtgatc gccacgccgt agttcctgaa aatggcgaac ggatacagca
     9961 gccctttcag aaaattcgga ttcgccgcgc tgaacagaac ggccagcgca aaccatccgg
    10021 tttcgcgaac gcgtttttgg ggctcactgc caatcaggcg ctcgatcagg aacaggaaca
    10081 gcatgccgag gccgaaaatg aaatagatgt gcgcattggc ccagaggagc atcagcggag
    10141 gaatcacaaa acggggccag cgttttcctt ttcgcacctg attaagaaga agcatcagca
    10201 gcgcgacgtt caggtatgtg aacagttccg gccggatgtg cgcgcggaaa ccgagaatca
    10261 ccgcggagaa aattcctgcg agccagtaca gattgaactc tccgcgcggc ttccgcgcca
    10321 gcatcgcgca gagcaggccc agcgtcatca tgatcatctt ccacgcgatc aggccgttca
    10381 ggccggctgc ccggtgcgcc agataaagga agatttcgga gagccagtgg tgattgatga
    10441 acggaaattc cggatgcgtg taggtcagca gattggtctg cggcaccgcc ctttccgcga
    10501 ggatgatttt tcccagcagc aggtgccgtc cgaggtcctc tgtgaagctg atgcccggcg
    10561 tgcaggccag caggagcatc agcagggcgc atcccgcaag gcccagagcg aaatgcgctt
    10621 tgtccccccg gctcatgttc atccgggcac aatgaaggga acagggcagc ccatgcctgc
    10681 tgcgacggcg cggaagagct cgccttcttc gcgcgtgacg gtgcggtcat gggacaggca
    10741 cgccatgcag gcggcgagca cctgtttctt gatgcccggt tcggcccggg tgagctgatc
    10801 cgccgcggca tccacgctgt cgaaggtgca gcgctccgcc gggagaagtt cgaacaggtc
    10861 aggcggcgcg gacagactct tgatcgcgtg ctggaaggcc tgccgggccg ccgcctcatc
    10921 atcgcttccc acgtgggcga tgctggaaag gatggtggag acgccgggag ccagcgccct
    10981 gatggcgtaa accggcgcgg tgcggaacgg ccgcgggccg tagtgatcct tgagcatgcg
    11041 cacaagcagg tgctgaatca tgtattcaaa caggtccagc tctccgtcgg cggcgatcag
    11101 ctgatccact gtgttcagaa aatggcggaa ctgcttttcc gaaagcgaca gcagggaatt
    11161 gcggcagatg tctatcagcg cgggctcgga ctccggcggc agggcagcca cggccggccg
    11221 gaactgatca gtctgctcgc ggacggccgc gtccgttgtg gatttcagcc agtccagctg
    11281 cttcgcaagg ggttccggct tgtcctcatc gagcagcagc gcgaagatca gggcctgcgc
    11341 gccgaacgga tcgcgcgccg cgttcagcac ctcatccgga acccgggcga gcacagcctg
    11401 caccgcctcc acgttttccg gccgcggagt gccgacggtt ttcccgaggg cctcggctga
    11461 gatcgccatc gctccgagcg cgggcgcgtt cagcacttcc ggcagccccg ggattttggg
    11521 cagcgccccc aatgcgctct tcttctccgg ttcagcagtc cccgccccgc tttccgcctg
    11581 ccatttttcg gccgcgatga actggccgtc ccaggaggga tccagctggc ggatgcgctc
    11641 ctccagcggc gggtgcgtgg ccatcatgtt gaacaggctc tgcctgaaac cgttgctgaa
    11701 gaacatatgg ctggcttctt cggcggccgc gtgcccgatg cgtgaaccca gcgagaaacc
    11761 gccgatcttc ttgagtgcgc gggcaatgcc cgtcgtgtcg cgcgtgaact gcacggcgga
    11821 ggcgtccgcc agatattcgc gctggcgcgc caccgcgctt tttatcagac gcccgaaaaa
    11881 gacaccgatg gagccgaaga ccagcatcgc aagggcgacc agcgcgatcc cgccgccgcc
    11941 tttccggttc ccgctgctgc gtcccatcga gcgcaggatg atcctcccga ggatgctgat
    12001 cagcaggatg ccgtggagga ggccgatcag ccggatgttc aggcgcatgt cgccgctcag
    12061 gatatggctg aactcgtggc cgatcacccc ctgcagctcc tcgcgcgaaa gcgcgtcgag
    12121 gcatccctgc gtcacgcaga caatggcgct gtcctgcgag tatccggcgg caaaggcgtt
    12181 gatgcctttt tcgttgcgct gcacgtaaac cggcggtggg ggcatgcctg cggcgatggc
    12241 catttcctcc accacattca gcaggcggcg ctccttgaaa tcgcgcgtgt tgggcggcac
    12301 gagactcccg ccgaggctct gggcgacggc tgcgccgccg ccgcggagcg ccgcgatctt
    12361 gtagagcgaa ccgagcagga ccagcagcag aacgccgagg gtcaccccca gaaacagctc
    12421 cggaatccag agcggctgag ggggtttcga gttgagccgc accagcaggg cgaagacata
    12481 ataaagcgaa ccgatcaggg ccgcgatggt cagcagaaaa tagaaaacca gtatccgggt
    12541 tttcttccgc gcgttctcct gcagatcaaa aaaatccatg gcctccggct cccgccagcg
    12601 ggctggcccg cggttttaga acgacacctt gggggcctgc ttctcggcct ctaccgtgac
    12661 ctcgaacagc gtcgccgggc cgaagttgaa catattggcg atgatgttct gcgggaacac
    12721 ctcgcgggcg gtgttgtagt gcatcacggc gtcgttgtag gcctggcgcg cgaaggacac
    12781 cttgttttcg gtcgtggtga gctcctcgct gagctgcatc atgttctggt tggccttcag
    12841 gtcgggatag gactcggaga gggcgaacag gcggccgagc gttccgccca gcgtgttttc
    12901 cgcgctcatc agcttcttca tggcctcggg gttcgacggg tcagcggcga ccgactggtt
    12961 ggcggaagcg gcggcgttgc gggcgctgat cacggcttca agcgtttcgc gctcgtgttt
    13021 catgtatccc ttggccacct cgaccaggtt gggaatcagg tcatagcgcc gcttgagctg
    13081 cacatcaatc tgggcgtagg cgttcttgaa gcggttcttc agcgcgacca gcttgttgta
    13141 catgccggcg acgattatga tcaggacgac aatgacggcg agaataatca aacctgtgct
    13201 cataactttc tccttttcag tttgaactcc aaatcaggcc cgcagtatcg gacacccggc
    13261 cctcatagtc acgcggaaaa tttccctccc gccccccgcg cttcatccgg cttcatccgg
    13321 tttcatcccc cgcatcgaaa cacaatatat cggtatccga tatatcggat accgatatat
    13381 cagcagatgt gatccggaag cgcggttcgt ttttggatcc gggttcgcgc atcagtttgt
    13441 tcttgagttc ggtgtcggac tgtctatgtg aaacggcgct gcgcgatgtt tctgaatgct
    13501 tttttcccgc cttcctgagt tcctgattcg gaaaatcata tctggaactc atgaactcag
    13561 gaagctatgg aaaggatcag ccgcagggta acgcagtttg gacatcaacc atgaagatcg
    13621 tgaagcagga aagaagctct gacgggagac tgttcttcat gtccttcttt tcttcatggt
    13681 gaaatattct tcctgtccca atttctaaac ggcttcaaga agtattggac aggatcaacg
    13741 ggatgggaac atacaccaga aaataatcga acgcagttca acatttggta tcccgtaaat
    13801 cctgttgatc ccgtcaaata tcttcatctg gaactcatga actcaggaaa tgtctgaaag
    13861 gaaactgtgt gtttagcttt ttgccagcag ttctgccacc gcgccgcgat ggttctcccg
    13921 cagagtattg agaatgcttc tttgccaagg gcgaggaa
//
LOCUS       DAOQWR010000060        13646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5001_length_13646_cov_5.696417, whole genome shotgun sequence.
ACCESSION   DAOQWR010000060 DAOQWR010000000
VERSION     DAOQWR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5001_length_13646_cov_5.696417"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKK36_06270"
     CDS             complement(<1..539)
                     /locus_tag="PKK36_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94200.1"
                     /translation="MSRLFKYVVLIVLGVLALGDGRLYAEDFRTVAAGDLKSNPKQYW
                     ATGIIFKDVLTAYPAGKKIQIENEDFVAFQTRTLGVCYADAPLAAALMVLPLDREYLF
                     RGTVLTRSRFIESAGRRTLPAHEQDNLFMVGMLSMLDVVFGLPLAKALAKLPLPDHLT
                     RAILGYEGQIGMLLSLAEA"
     gene            complement(617..1264)
                     /gene="nth"
                     /locus_tag="PKK36_06275"
     CDS             complement(617..1264)
                     /gene="nth"
                     /locus_tag="PKK36_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNR94201.1"
                     /translation="MKPTPALRKKGAAILKALRRLYPQAHCELVFGSPLELAVAAILS
                     AQCTDRRVNMVTPALFKKYRTAEDWAHADLAVLEKDIHSTGFFRNKAKNIKALGRALC
                     DRYGGALPSELETLITLPGIGRKTANVLLISAFGKPGVTVDTHCTRVSRRLGLTRSLS
                     PEKIEMDLKNLFAEKDWADLSHCLVFHGRYCCKARKPLCAQCPLAKLCPSCELEN"
     gene            complement(1335..3437)
                     /locus_tag="PKK36_06280"
     CDS             complement(1335..3437)
                     /locus_tag="PKK36_06280"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR94202.1"
                     /translation="MNNTTFVEVDIGGKIVRFETGLLARQAAGAITLQQGENVLFSAV
                     TCTKTPREGIDFFPLQIEYRDKFYAAGRFPGGYFKREARPSEKEILIARVTDRPIRPL
                     FPEGYRNDVQIVNTLLATDGDTESDAMSVNAASAALVISEIPFMGPIAAVKVGRINGE
                     FVLNPTQKQLAESDLDLTYAGTRDHMLMVEGSAKEIPEADMLAAMKFGHPFVVKLIDA
                     QLELRKKLGLPDKVITEAAVETPELAKARELGGQELADVLTIKGKLDRQEKSGLFFEK
                     LKAQMLEAFPEMTAEQYMQTFDKLQIETVRKNAVEKGVRIDGRGFDELRPLAGQVGLL
                     PRVHGSALFNRGETQAMGVVTLGTSSDTQSMDAITGGDTEKKFMLHYNFPPYSTGEVG
                     RFGTTKRREIGHGALAERSIAPVIPEDYPYTVRVVSEIMGSNGSSSMASICVGTLSLM
                     DAGVPIKKPVAGISIGLITDEKSDKAILLTDILGAEDHCGDMDFKFAGTRQGITGFQV
                     DLKIPGLRWDIIEGALERARVARGKILDLIESVLPAPRAELSPYAPRIHTMKIDPEKI
                     GALIGPGGKNIRRITDETKAQIDIEDDGTVRIFSNNNESMQAAIREVSLITAEAEEGK
                     IYRGTVTGIKDFGAFVEILPGKDGLVHISELADHRVKTVEDICKVGDSMWVKCIGIDD
                     RGKIKLSRRAALKEQQEG"
     gene            complement(3594..3857)
                     /gene="rpsO"
                     /locus_tag="PKK36_06285"
     CDS             complement(3594..3857)
                     /gene="rpsO"
                     /locus_tag="PKK36_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017510024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNR94203.1"
                     /translation="MDVETKNVIKQDYKLHDRDTGSVEVQVALLTKRIHDLTEHLKDN
                     KKDHSSRRGMIILVSRRRRLLDYLKRKDSQRYAELIKRLSLRR"
     gene            complement(3964..4824)
                     /locus_tag="PKK36_06290"
     CDS             complement(3964..4824)
                     /locus_tag="PKK36_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNR94204.1"
                     /translation="MGTRIIAIANQKGGVGKTTTTVNLAACLTVLKKNVLLIDLDPQA
                     NATSGVGLEKTPEMSIYKALLEDGRARDVIQATEYKRLDMIASELDLAGAEVDIARTD
                     NYLHRLRNALADLKNAGLYDFILIDCPPSLGILTMNALTAAHSVLIPIQCEYYALEGL
                     TIMTEIIRKLHQSGANPELDIEGIVMTMYDMRTNLSQAVVQEVSQHFGDKIYETLIPR
                     TVRLSEAPSFGKPIIAYDSRSTGAVAYLQLAKEVIARCKAEDARAAAEESASTPPPAP
                     ALSPEVSSGV"
     gene            4961..6040
                     /locus_tag="PKK36_06295"
     CDS             4961..6040
                     /locus_tag="PKK36_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNR94205.1"
                     /translation="MNSSCFRRALTGAVFCFAFTAAAEVKLPGTKPDTEIRIWGQVNR
                     AVLYADNGTYSEWFHVDNDSMPSRIGLAGDYKPPEWAGWTFGGQIELGFKSDNSCDVE
                     FESDDPEFKVDGRKIEAWAKNPRFGALSIGQGDMASNGTAEEDLSGTYVIAYSQVRFA
                     AGSLIFGDQTNSPAIKDVFNNFDGLHRDDRVRYDTPAWKGFTISASAGGHDFYDAALR
                     HKMEIDGHKIASAVSVAQKDTAVDQYCGSVSVLLKCGLNFTAAAGGQNVEDDYSPLSF
                     YGKIGWKMDLFSCGVTCVAADYSHNEEISRADDKAESYAIVLNQRVEPILSDVYVSVR
                     NYSLDRPGEDYDDILTFMAGIKLAF"
     gene            complement(6696..7427)
                     /locus_tag="PKK36_06300"
     CDS             complement(6696..7427)
                     /locus_tag="PKK36_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94206.1"
                     /translation="MKTLTAFLLLLPAALFAAGQTSETFGVKADVLNTADPSLSAGSA
                     SLQGSVSVSQHSLVEVQTSASFTVHSGYQASTVGFDSDYDGIPDSLDADDDGDGVPDS
                     SDGFVYDTDNDGERNLADDDDDNDLLADTEEYSAGTSLILVDTDGDTHTDYEEVVVTG
                     TDAVNSGDYLQVGGIVRPGANGTVISWNAVTGKIYHISGRSSLAGEGIWNDLSGAVTA
                     SVESMSWTNTGPVATQLNYRISVPY"
     gene            complement(7430..9667)
                     /locus_tag="PKK36_06305"
     CDS             complement(7430..9667)
                     /locus_tag="PKK36_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94207.1"
                     /translation="MKAVFRFFVVLSVVPLCVTGASINYQGRIAAGGSDYTGPAWIKA
                     RAISMLDENLWSNDGSAGTGEPSGSVQIEVNNGLFNIELGNPFIPGMTAMDWKVFRDS
                     ECSLRIWFSTNSTAYEQLSPDVSIRPATLEKLSSGRTVFVDDSDSADFNNLQDALNAV
                     YNDEAESIIILPGWYPVTTPLTFSTQRFVSITGWGNRDEINIECTNGPAMYLQSARLE
                     NISVSGSPAISDNDPSVAASYWWQADHCVFRNNSAGAPEVPAMEFSGDGQAILFDCRI
                     ENQSANSGPALALGGNVDLAMSHCSLWSGSGGGSAALVKESPVMRAVNCDFFSDDNLS
                     LLVTAPSSARFDVQDCRMHAGIYFSVTGGVNSAGGDFIQCGIGNENGADAAIHINGGC
                     GWLNFDNCNIWTEDAHGIYQYSEWGYNTGVQMRNSSISVYDTGGDPGHGVFIGNSSLN
                     DMGSASINLMNCRISAFSGRGIDSDYASVQLSDTHVQSFENFAINCSSSDLSVEHSDV
                     RGNSGGILAAYSEIEISHSSIEGSEYGIYMGECMADVSSSMVVGGLNDGILAAGSGTN
                     ECELFITRSDITGSDSDEGALAGCAIRTDFTPETPMVIFHSTLDNFSEFPTLDLNGGM
                     NIIEESIILTEAGPAVSITRPDLFIFEHTKFLNVGDGNTNALLLLCENDGEPIPPVVN
                     GCVFRTLFGAAMAAVDMNPTCSATNSAIIMADCYLGNVTFNTNAIALVPVDDTTLPYG
                     NIIGF"
     gene            complement(9758..10219)
                     /locus_tag="PKK36_06310"
     CDS             complement(9758..10219)
                     /locus_tag="PKK36_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR94208.1"
                     /translation="MENLKVRQVIESKGSGKLFTIRENCTLREMVRIACEMKIGALLV
                     ADEEENLLGIVSERDIMRQCHARADFDKVTVGDIMTRNLLTVDPEENIQEAMDVMIKK
                     KIRHLPVLHKGKIEGLITVRDILHAMRDADAREAELLVNYLQSEMDQRSQP"
     gene            10332..11363
                     /gene="queA"
                     /locus_tag="PKK36_06315"
     CDS             10332..11363
                     /gene="queA"
                     /locus_tag="PKK36_06315"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNR94209.1"
                     /translation="MNTADFDYNLPAELIAQEPPPSREDARMMVVRRDTRTWEHRRVG
                     ELPEFLRAGDLLVLNDTRVIPARIFGRKESGGRVEFLLLEELESNVWDVLMRCSRRPK
                     PGARVTLESGRAEAEILEDGAMGRARIRIHCNGPFLNILEEDGHVPLPPYITRPDSPA
                     RDRDRYQTVYARAPGAVAAPTAGLHFTGELFQALEKRGVGSAHITLHVGIGTFRPVAS
                     DRVEDHVMDEERYEISPAAADKINAARKAGGRIVAVGSTSVRTLEHCANADGTVCAGS
                     GRSGLFIHPPYTFRIVDAMLTNFHLPRSTLLMMVCALAGRDFMMKVYEEAVRERYRFF
                     SYGDCMLIL"
     gene            11399..12178
                     /locus_tag="PKK36_06320"
     CDS             11399..12178
                     /locus_tag="PKK36_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94210.1"
                     /translation="MAAWFILGTFFLLLARLPFFAAPMIPHYLEFNPGVALVPLLGVF
                     WGPAAVWSALAASLLGDWITGIWSSLSIFKAVGMASMAFTAQYLWDTGAPNAYAGRKH
                     THSWVSTIHYLHAATAGCFVSAAWTGLGADMSRLYPFPYISALVLVHHLIFANLPGIA
                     FYRFTVREVLPDWGNWRSAMENEYPIPVPTHFTSVAVWVTGTGACVAGWVVSAVFYRI
                     TPLKAYVLGNCCGTGLAAILILFLAAHSFLLLRGPRHKKKR"
     gene            complement(12289..13635)
                     /locus_tag="PKK36_06325"
     CDS             complement(12289..13635)
                     /locus_tag="PKK36_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNR94211.1"
                     /translation="MWPKMAKSFLVFTHLGVIEGFLSFPMKRLPQSLKAAFLRVMRER
                     AVPVEQRQDYLKWLRFYLDFCLKYRHPPRDPDSLQLFLHKMAAKNQSAAQQTQAAACI
                     GLYYNLMKTWAAAPESSATVENKRKPWDECYTLLKQEIKLRQYSPKTLQTYATWIRQF
                     QKFIGNKPPVELVADDARRFLTHLAVEKRVTASTQNQAFNALLFLYREILKVDYDLKD
                     TVVRARRTRYIPVVLSREEVARILSEMSAPHLLVVQLLYGCGLRMSECLNLRVHCFNF
                     DDAVLTIHDGKGKKDRTVPLPKSLIPELKAHMEKVVNLHEKDLAAGYAGVFMPGALDR
                     KWKSAAREFIWQWFFPARTLTLIPEENERRRYHMHESELQKALRAAARKARIPKRVTC
                     HTFRHTFASHLLAANYDVRTIQQMLGHSDIRTTMIYTHTIPSRTLKEQVSPLDFPA"
BASE COUNT         2767 a         3959 c         3903 g         3017 t
ORIGIN      
        1 aaggcttcgg ccagcgaaag cagcatgccg atttgaccct catagccgag gatagcccgt
       61 gtcagatggt ccgggagcgg caacttggcc aatgccttgg ccagcggcag accgaagacg
      121 acatccagca tcgacagcat accgaccatg aagagattgt cctgctcgtg ggcgggcagg
      181 gtacggcgac cggccgattc gatgaaacgg ctgcgcgtca gcacggttcc ccggaaaagg
      241 tattcccggt ccagcgggag gaccatcagc gccgccgcaa gcggggcatc cgcatagcaa
      301 acccccaatg tgcgggtctg aaaggcaacg aagtcttcgt tttcgatctg gatctttttc
      361 ccggcgggat aagcggtcag cacatccttg aagatgatcc cggtcgccca gtactgcttg
      421 gggttgcttt tcaggtctcc ggccgcaacg gtcctgaaat cctcagcata aagacgtccg
      481 tccccgagtg ccagaacacc gagaacaatc agcaccacat atttgaacaa tctgctcatg
      541 gcgaaaataa acgataagcg cttaatcggg tcaagataaa ccattcgggg gcaaaacacc
      601 accccattgc acatctttaa ttttcaagct cacacgacgg gcacagtttc gccagcggac
      661 actgcgcaca caacggcttg cgggccttgc agcagtagcg gccgtggaag accaggcagt
      721 gggacagatc ggcccagtcc ttttccgcaa agaggttttt caggtccatt tctatcttct
      781 cgggactgag actgcgcgtg agtcccagcc ggcgggacac ccgtgtgcag tgggtatcca
      841 cggtgacacc gggcttcccg aaggccgaaa tcagcagcac gttggccgtc ttccggccaa
      901 tccccggcag agtgatcagt gtttccagtt cagaaggaag cgccccgccg taacggtcgc
      961 acagcgcccg gcccagcgcc ttgatgttct tcgccttgtt gcggaaaaaa ccggtggaat
     1021 ggatatcttt ctccagcacc gccaggtcgg catgggccca gtcctccgcg gtgcggtatt
     1081 tcttgaacag cgcgggcgtg accatgttca cgcgccggtc cgtgcactgc gccgacaaga
     1141 tggccgccac ggccagttca agcggagaac cgaacacaag ctcgcagtgc gcctgcggat
     1201 agagccggcg cagggctttc aggatcgcgg cacccttttt tctgagcgca ggagtcggtt
     1261 tcataacagg tcttttaaat cgcaggggct gcccgacggc agcccctgct ccggtttcaa
     1321 ctgttgcgcg gcaatcagcc ttcctgctgc tctttcagag cagcccggcg gctcagtttg
     1381 attttgccgc gatcatcaat tccgatgcat ttcacccaca tggagtcacc caccttgcag
     1441 atgtcttcaa cggttttgac gcggtgatca gccagttcgc tgatgtgcac gaggccatcc
     1501 ttgcccggaa gaatttcaac gaaagcgccg aaatccttga tgccggtgac ggtgccgcgg
     1561 tagatcttgc cttcctcggc ttcggccgtg atcagcgaga cctcgcggat ggcggcctgc
     1621 atggactcat tgttgttgct gaaaatccgc acggttccat catcctcgat gtcaatctgc
     1681 gccttggttt catcggtgat ccggcggatg ttctttccgc ccgggccgat cagcgcaccg
     1741 atcttctcgg ggtcgatttt catcgtgtgg atgcgcggag catacggaga cagttctgct
     1801 ctcggagccg gcagcacgga ctcgatcagg tccaggattt tgccgcgggc cacgcgggcg
     1861 cgttccaggg cgccttcgat gatgtcccag cgcaggccgg ggatcttcag gtccacctgg
     1921 aagcccgtaa tgccctgacg ggtaccggca aacttgaagt ccatgtcgcc gcaatggtct
     1981 tccgcgccga gaatatcggt cagcagaatg gccttgtcgc tcttttcatc tgtgatgagg
     2041 ccgatggaaa tgcccgctac aggctttttg atgggcacac cggcatccat gagggaaagc
     2101 gttcccacgc agatgctggc catggaggac gagccgttgg atcccatgat ttcggaaaca
     2161 acgcgcacgg tgtacggata atcttccgga atcaccggcg cgatggaacg ttccgcaagg
     2221 gcgccgtgac cgatttcgcg gcgcttggtt gtgccgaagc ggcccacttc ccccgtgctg
     2281 tagggcggga agttgtaatg aagcatgaat ttcttctcgg tgtcgccgcc cgtaatggca
     2341 tccatgctct gcgtgtcgga gctggtgccc agcgtcacga cacccatggc ctgggtctcg
     2401 ccgcgattga acagcgccga accgtggacg cgcggaagca ggcccacctg accggccagc
     2461 ggacgaagct cgtcgaagcc gcggccgtca atccgaacac ccttttcaac ggcgttcttc
     2521 cggaccgttt cgatctgcag cttgtcaaag gtctgcatat actgttcggc cgtcatttcc
     2581 gggaaggctt cgagcatctg ggccttcagc ttttcaaaga acaggccgga cttctcctgc
     2641 cggtccagct tgcccttgat ggtgagaaca tccgcgagtt cctgtccgcc cagttcacgg
     2701 gcctttgcca gttccggggt ttcgacggcg gcttctgtga tcaccttgtc cgggagcccg
     2761 agctttttgc gaagctccag ctgcgcatca atcagcttta caacaaacgg atgcccgaac
     2821 ttcatggcgg ccagcatgtc cgcttccgga atctccttcg cgctgccttc caccatcagc
     2881 atgtgatcgc gggtgcccgc gtaggtcagg tccagatcgc tctccgcgag ctgcttctgg
     2941 gtcgggttta gaacaaactc tccgttgatc cggccgactt tcaccgcggc gatcgggccc
     3001 atgaagggga tttccgaaat caccagcgcc gcactggcgg cattgacgct catcgcatcc
     3061 gattcagtgt cgccatcggt cgccagcagg gtgttcacaa tctgcacatc gttgcgatag
     3121 ccttccggga acagggggcg gatcggacgg tcggtgactc gcgcgatcag aatttccttc
     3181 tcgctcggcc gcgcttcgcg cttgaaatag ccgccgggga accggccggc cgcgtaaaac
     3241 ttgtcgcggt attcaatctg caaagggaaa aagtcaatcc cttcgcgcgg ggttttggtg
     3301 caggtcacgg ccgaaaacag cacgttttcg ccctgctgca gggtaatggc tccggcagcc
     3361 tgccgggcca gaagaccggt ttcaaaacga acgatcttcc cgccgatatc aacttcgacg
     3421 aatgttgtat tattcatatt ttttctcctg attttgaagt cttgccgagg cagagaacac
     3481 ccctggacat ccggtttgcg ctgaacatca ggcatgcggg aaacccgggg gcagtcagcc
     3541 cgtttttcgg ggacgaattc atgacttggc tataaggcct gcaagcaaca ggcctaacgg
     3601 cgcaggctca ggcgcttgat cagctcagcg tagcgctggc tgtccttgcg cttgagatag
     3661 tccaaaagcc gccggcggcg gcttaccaga ataatcatgc cccgacgcga gctgtgatcc
     3721 tttttgttgt ccttcaggtg ctcggtcagg tcgtgaattc gcttcgtaag cagggccacc
     3781 tgaacttcca cggatccggt gtcccggtcg tgcagtttgt aatcctgctt aataacattt
     3841 ttggtctcta catccataac tacagcagac ctctttcttt tccttttctt tcttttgctt
     3901 ttttatggtc ggcggactct atgaaacctc ttcggttttg taaagcctga tatgggggtt
     3961 ttttcagacc ccggaagaga cctccgggga cagcgcaggc gcaggcggcg gagtggaagc
     4021 gctttcttcc gcggcagccc gtgcatcctc ggccttgcag cgcgcgataa cctcttttgc
     4081 cagttgaaga tacgcaaccg cccccgtgga ccggctgtcg taggcgataa tgggtttgcc
     4141 gaagctgggg gcctcgctca gccgcacggt ccggggaatc agcgtttcgt agattttatc
     4201 tccaaagtgc tgggaaactt cctgcaccac cgcctgcgaa aggttcgtcc gcatgtcata
     4261 catggtcatg acaatgccct cgatatccag ttcgggattc gcgccgctct gatggagctt
     4321 gcgtatgatc tccgtcatga tggtcagccc ttccagcgca taatattcac actggatcgg
     4381 gatcagcacc gaatgggccg ccgtcagggc attcatggtg aggatgccga gcgagggcgg
     4441 gcagtcaatc agaatgaaat catacagccc ggcgtttttc aggtccgcaa gcgcgttgcg
     4501 aaggcggtga agatagttgt cggtccgcgc aatatcaacc tcggctccgg ccagatccag
     4561 ctcagaggcg atcatgtcca ggcgcttgta ttccgtggcc tgaattacat cccgtgcgcg
     4621 gccgtcctcg agcagggcct tgtagatgct catctccggc gtcttctcca gcccgactcc
     4681 gctggtcgcg ttggcctgcg gatccagatc aatcagaaga acattcttct tgagcacagt
     4741 caggcaggcg gccaggttaa cggtcgtggt ggtttttccc actccgccct tctgatttgc
     4801 gatggctatt atccgggttc ccataattcc cggcgagcat agacggacat ccgcccggga
     4861 tcaagaccgg atgcaggcgg tttgacatcc gacaggcatt tttcttgcca aaaccggcat
     4921 ccgttaacaa taatccgatc aactgaatgg aggtttccgg atgaacagca gctgtttccg
     4981 cagagctttg accggcgcgg ttttttgttt tgcgttcacc gccgccgcgg aagtgaaact
     5041 tccgggaacc aaacccgata cggaaatccg gatatggggg caggtcaacc gcgccgtgct
     5101 ctatgcagac aacgggacct attccgagtg gttccatgtg gacaacgaca gcatgccctc
     5161 gcgaatcggc ctcgccggcg attacaagcc cccggaatgg gccggctgga cctttggcgg
     5221 ccagatcgag ctgggcttca agtccgacaa ttcctgcgat gtggaattcg aaagcgacga
     5281 tccagagttc aaggtggacg gccgcaaaat cgaggcatgg gcaaaaaatc cccgcttcgg
     5341 cgcactttca atcggacagg gcgacatggc ctccaacgga acggccgaag aggatttgtc
     5401 cggcacctat gtcatcgcat acagccaggt gcgcttcgcg gccggcagcc tgattttcgg
     5461 agatcagacc aattctcccg ccatcaagga cgtgttcaac aatttcgacg gactgcatcg
     5521 cgacgaccgg gttcgttacg acacgccggc gtggaaagga ttcacgattt cggcctccgc
     5581 cggcgggcac gacttttatg atgccgccct tcggcacaag atggaaattg acgggcataa
     5641 aatcgcctct gctgtttcgg tggcgcagaa ggacacggcc gttgaccagt actgcggttc
     5701 tgtttcggtg ctgctgaagt gcggactgaa cttcaccgca gcggcaggag gccagaatgt
     5761 cgaagacgat tattcccctc tgtcctttta cggaaaaatc ggctggaaaa tggacctctt
     5821 ttcctgcggc gtcacctgcg tggcggctga ctattcacac aacgaggaaa tctcgcgcgc
     5881 ggatgacaag gcggaatcgt atgcgattgt tctgaaccag cgcgtggagc cgatcctctc
     5941 ggatgtctat gtgtcggtcc ggaactactc tctcgacagg cccggcgagg attacgatga
     6001 catcctgacc ttcatggccg gcattaagct tgcgttttga gggctgcggc caccgcccgc
     6061 ggctacagca gagagtgcgg gcggtgaccg cggtgattca ggataagaca atttgaaaac
     6121 tgtagccgcg ggcggtgacc gcggcattgt tattctctca atcccgacgg cgcatcaaaa
     6181 ttcacgaatc cctgatacgg ccactcttcc ccccgcttca caaggcccgc ccggagagga
     6241 ttgtcgcgaa catatatcca cttttctctg tacgattcgc ccgaccgcat gacatggtca
     6301 aaatattcct tctgccagac cggagatgaa actgatccgc tttggcaaat agctttcgaa
     6361 gtccattctt tccagccgcg cataaagaca gaaagcgagt gcccggttcc gggactgctc
     6421 tctgcgcaaa aaaaatgcac atgatccggc ataatcatat aacgcccgac aagccatcca
     6481 tgcaggtctt tcgcggacga cagttcgcgg cggattattt ccgctgtatt ttgagaagcc
     6541 agaatccttt gccgattata tgtgcaggcg gtgataaaat atactgcatt gggaatccac
     6601 acccggctga gacgctttaa atgtttatgc tctgtcatgt cctgaatttg ccgcggtcac
     6661 cgcccgcagc tgcagcactc aatccggggc tttgatcagt acggcacgct gatgcggtag
     6721 ttgagctgcg tggcgacggg accggtgttg gtccagctca tggattcgac cgaagccgtg
     6781 accgcgccgg acagatcgtt ccagattccc tcgccggcga gagaactgcg gccggagata
     6841 tgatagattt ttcccgtgac ggcattccat gagatcaccg tcccgttcgc gcccggcctg
     6901 acgattccac ccacctgcag ataatcgccg ctgttcaccg catctgttcc ggtgacgaca
     6961 acttcttcgt agtccgtgtg ggtgtcgccg tccgtgtcga caagaatgag ggaagtgccg
     7021 gcggaatatt cctctgtgtc cgccagcaga tcgttgtcgt catcgtcatc ggccaggttg
     7081 cgctcgccgt cgttatctgt gtcataaaca aacccgtcgg aggagtccgg aactccgtcg
     7141 ccgtcatcat ccgcatccag agaatcggga atgccgtcat aatcggaatc aaaaccgacg
     7201 gttgaggcct ggtagccgga atggactgta aaggacgcgc tggtctgaac ttcgaccaga
     7261 gaatgctgcg acacggagac ggaaccctgc agggatgcgc ttccggcgct cagcgagggg
     7321 tccgccgtat tgagcacatc cgctttgact ccgaatgttt cgctggtctg gcccgccgcg
     7381 aacaacgcgg ccggcagcag cagtaaaaat gcagtcagcg ttttcatggt cagaacccga
     7441 tgatgttccc gtaaggcagg gtggtgtcgt ccaccggcac cagcgcaatg gcgttggtgt
     7501 tgaatgtgac attgcctaga tagcagtccg ccatgatgat tgcgctgttc gtagcagaac
     7561 atgtcggatt catgtccacg gccgccatgg ccgcgccaaa aagggttcgg aaaacacacc
     7621 cgttgacgac aggcgggatg ggctcgccat cgttttcgca cagcaggagc agcgcattgg
     7681 tgttgccgtc gcccacattg aggaatttcg tgtgttcaaa tatgaacagg tcgggccggg
     7741 tgattgagac ggcgggaccg gcttctgtga gaatgatgct ttcttcaatg atgttcatgc
     7801 ctccgttcag atcaagtgtc gggaattcgc tgaaattatc cagcgtgctg tggaagatca
     7861 ccatgggtgt ttcgggcgtg aaatctgtcc gtatggcgca gccggcgagc gccccttcat
     7921 ctgaatcact tccggtgatg tcggagcggg tgatgaacag ctcacactca ttagtgccgg
     7981 acccggcggc aagtatgccg tcattcagtc cgccgacaac catggaactg ctcacatccg
     8041 ccatgcattc tcccatataa attccgtatt cactcccttc aatggaagaa tggctgattt
     8101 cgatttctga atatgcggcc agaattcctc cgctgtttcc tctcacgtct gaatgctcga
     8161 cgcttaagtc ggagctgctg cagtttatgg caaagttttc gaagctttgc acatgcgtgt
     8221 ccgagagctg aacggatgca taatcactgt cgatccccct tcccgaaaaa gcggaaattc
     8281 tgcagttcat gaggttgatg gaggcggatc ccatgtcatt caaagaggag ttgccgataa
     8341 ataccccatg tccaggatct ccccccgtat cgtaaacaga aatgctgctg ttccgcatct
     8401 ggacacctgt gttgtatccc cactccgagt actgataaat gccgtgcgca tcctcggtcc
     8461 agatattgca gttgtcgaaa ttaagccatc cgcagccgcc gttaatgtga atcgcggcat
     8521 ccgcaccgtt ttcgttacca atgccgcact ggataaaatc cccgccggcc gaatttactc
     8581 ctccggtaac cgaaaaatag attccagcgt gcatgcggca gtcctgaacg tcgaatctgg
     8641 cgctgctggg ggcggtaaca agcagcgaca ggttgtcatc actgaagaag tcgcagttga
     8701 cagccctcat gacggggctt tccttcacca aagccgcgct cccgccgccg cttcccgacc
     8761 agaggctgca gtgggacatg gccaagtcca catttcctcc aagcgccagc gcggggcccg
     8821 aattggcgga ctggttctca attcggcagt caaagagaat cgcctgcccg tcgcctgaaa
     8881 actccatggc cgggacttcg ggggcacccg ccgaattgtt ccggaaaacg cagtggtcgg
     8941 cctgccacca gtaggatgcc gccacactcg ggtcattgtc tgagatcgcg gggcttccgc
     9001 tgacagagat gttttccagc cgggcgctct ggagatacat ggccgggccg ttggtgcact
     9061 cgatgttgat ttcgtcccgg ttgccccagc cggtgatcga aacgaagcgc tgcgtgctga
     9121 aggtcagcgg cgtcgtgacc ggataccagc cgggcaggat gatgatgctt tcagcttcgt
     9181 cattgtacac cgcattcagc gcatcctgca gattgttgaa gtcggccgaa tcgctgtcat
     9241 ccacgaagac ggtccggccg gaactgagtt tttcaagagt ggcgggccgg atgctgacat
     9301 ccggcgaaag ctgctcataa gctgtgctgt ttgtggaaaa ccatattcta aggctgcatt
     9361 cagagtcgcg gaaaaccttc cagtccatcg cggtcatgcc ggggatgaac ggattgccca
     9421 gttcaatatt aaacagaccg ttgtttactt cgatctgcac ggaaccggag ggctctccgg
     9481 ttcccgcgga gccgtcattg gaccacaggt tttcatcgag catggaaatg gcgcgcgcct
     9541 tgatccatgc cgggccggtg taatcgctcc ccccggcggc gatgcgcccc tgatagttga
     9601 tggatgcccc cgtcacacac agggggacaa cggacagcac aacaaaaaaa cgaaataccg
     9661 ctttcatacc gcctccttgg ttcctgggga cagcccgatg catgtattta gcggaaacgg
     9721 gggcggattt gtcaacaaaa ttacgccaaa cgggagctca gggctggctg cgctggtcca
     9781 tttcgctttg cagataattc accagcagtt cggcctcgcg ggcatcggca tcgcgcatgg
     9841 catgaagaat gtcgcgaacg gtgatcaggc cctcgatttt tcccttgtgc agaacgggaa
     9901 gatggcggat tttcttcttg atcataacat ccatggcttc ctggatgttt tcctcgggat
     9961 ccacggtcag caggttgcgg gtcatgatgt cgcccacagt gactttgtcg aaatcggccc
    10021 gggcatggca ctgccgcatg atgtcgcgtt cgctgacaat gccgagcaga ttttcctcct
    10081 cgtccgcaac cagtagcgcg ccgattttca tctcgcatgc aatccgcacc atttcccgca
    10141 gagtgcagtt ttcgcggatg gtgaacagtt tgccgcttcc cttgctttcg ataacctgcc
    10201 gcaccttcag attttccatc gcgatagtct ccggatttca gaaatatact ggaaagagaa
    10261 catttttcac tccggcgcgt caaattcttt cgaccggaag cggtgcgcat ggtatggatg
    10321 gccggcagaa gatgaataca gccgatttcg attacaatct cccggcggaa ctgatcgcgc
    10381 aggagccgcc gccgtcccgc gaggacgcgc ggatgatggt ggtgcgccgc gacacccgga
    10441 catgggagca ccgccgcgtg ggtgagttgc cggaattcct gcgcgcgggc gacctgctgg
    10501 tgctgaacga cacacgggtc atccccgcaa ggattttcgg caggaaggaa tccggcggcc
    10561 gggtcgaatt tctcctcctg gaggagctgg agagcaatgt ctgggatgtc ctgatgcgct
    10621 gttcgcggcg gccgaagccg ggcgcgcggg tcacgctcga atcgggccgg gccgaggccg
    10681 aaattcttga agacggcgca atgggccgcg cccggatccg gattcactgc aacggcccct
    10741 tcctgaacat tcttgaggaa gacggccacg tgccgctgcc gccctacatc acgcgaccgg
    10801 actcccccgc ccgcgaccgg gaccgctatc aaacggttta cgcgagagcc cccggcgccg
    10861 tggccgcgcc gacagccgga ctgcacttca ccggcgaact cttccaggcg ctggaaaaac
    10921 gcggggtggg cagcgcccac atcaccctcc atgtaggcat cggaacattc cggcccgtcg
    10981 ccagcgaccg ggtggaggac catgtgatgg atgaagagcg ctacgaaatt tccccggcgg
    11041 cggcggataa aatcaatgcc gcgcgaaagg ccggaggacg gattgtcgcg gtcggaagca
    11101 cctcggtgcg gaccctggag cactgcgcaa acgcggatgg aaccgtttgc gcgggaagcg
    11161 gacgatcggg gctgttcatt catcccccct acacgttcag gatcgtggat gccatgctca
    11221 ccaacttcca cctgccgcgc tccaccctcc tgatgatggt ctgcgcgctg gcggggaggg
    11281 atttcatgat gaaagtgtat gaagaagcgg tcagggagcg ctatcgcttt ttcagctacg
    11341 gggactgcat gctgatttta taatgagaaa tctcttcata atgtggaaat atccccggat
    11401 ggccgcctgg ttcattctcg ggaccttttt ccttctgctg gcgcggctgc cgttttttgc
    11461 ggcccccatg atcccgcatt atcttgaatt caatccgggg gttgcactgg ttccgctgct
    11521 gggggttttc tggggaccgg cggccgtgtg gagcgcgctg gcggccagcc tcctcggcga
    11581 ctggatcacc ggcatctgga gcagcctgtc catcttcaag gccgtcggca tggcctccat
    11641 ggccttcacg gcacagtatc tttgggacac cggcgcgccc aacgcctacg cgggccgcaa
    11701 gcacacgcat tcgtgggtga gcaccattca ctatcttcac gctgccaccg cgggctgctt
    11761 tgtttccgcc gcgtggaccg ggcttggcgc cgacatgtcg cgcctgtatc ccttccccta
    11821 catttccgcg ctggtccttg tgcatcatct gatcttcgcc aacctgccgg gaatcgcatt
    11881 ctaccgcttc acggtgcggg aagtgctgcc cgactggggc aactggcgca gcgccatgga
    11941 aaatgaatat cccattccgg tcccgacgca tttcacctcg gtggcggtat gggtcacagg
    12001 caccggcgcg tgcgtcgcgg gctgggtggt cagcgccgtc ttctaccgca tcaccccgct
    12061 caaagcctat gtgctgggca actgctgcgg cacgggtctt gccgcaatcc tgatcctctt
    12121 cctcgccgcc cacagcttcc tgctccttcg cggaccgcgg cataaaaaga aacgataaag
    12181 cgtccgctgt atggcgagat gagatgcggc agacagaatg gtaatttcca ctgagcggaa
    12241 gtatttcgga ggaaatatcc aggaattgga aaatcttgaa tgccccgttt aggctggaaa
    12301 atccagcggg ctgacctgtt ccttgagtgt gcggctggga attgtgtgcg tatagatcat
    12361 cgtggtgcgg atgtcactgt gccccagcat ctgctggatc gtgcgcacat catagtttgc
    12421 cgccagcagg tgacttgcaa atgtgtgccg gaaggtgtgg caggtcaccc gtttcggaat
    12481 ccgcgctttc cgcgcggcag cccgcagcgc cttttgcagc tcggactcat gcatgtgata
    12541 gcgccttcgt tcattctctt cgggtatgag agtcaatgtt cgggccggga aaaaccactg
    12601 ccagataaac tctctggcgg ctgatttcca cttgcggtcc agtgcgcctg gcatgaaaac
    12661 cccggcatag cccgcggcaa ggtccttttc gtgaagattc acgaccttct ccatgtgcgc
    12721 tttcagctcg gggatcaggg attttggcaa aggcacggtg cgatctttct tccccttgcc
    12781 gtcatggatc gtcaggactg cgtcatcaaa attgaagcag tggacccgaa ggttgaggca
    12841 ctcggacatc cgcagaccgc agccgtagag gagctggacc accagcaggt gcggagcgct
    12901 catctcggaa agaatccggg ccacctcctc ccggcttaat acaaccggaa tatatctcgt
    12961 gcggcgggcg cgcaccacgg tgtccttcaa gtcgtaatcg accttgagaa tttcacggta
    13021 gagaaacaaa agcgcgttga atgcctgatt ctgtgtggat gccgtcacgc gtttttcgac
    13081 cgccagatgc gtcaggaaac gccgcgcatc gtctgcaacc agttcgacag gcggtttatt
    13141 cccgatgaat ttctggaact gcctgatcca tgtggcatag gtctgcagcg ttttcgggga
    13201 atactgccgc agtttgattt cctgtttcag cagggtatag cattcatccc atggcttgcg
    13261 cttgttttca actgttgcgg atgattcagg tgcggcggcc catgtcttca tcaggttgta
    13321 atacagtccg atgcatgcgg cggcctgcgt ctgctgcgca gcagactgat tctttgccgc
    13381 cattttatgc agaaagagct gcaggctgtc ggggtcgcgc ggggggtgcc gatacttcag
    13441 gcagaaatcg agatagaacc ggagccattt gagataatcc tgacgctgtt ctacgggaac
    13501 agcccgctcc cgcatcactc ttagaaaagc cgcttttaaa ctctgtggca acctcttcat
    13561 gggaaaagac agaaaacctt ctatcacgcc taaatgcgtg aacacaagaa aggatttcgc
    13621 cattttgggc cacatttggg cccttt
//
LOCUS       DAOQWR010000061        13329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5142_length_13329_cov_5.567952, whole genome shotgun sequence.
ACCESSION   DAOQWR010000061 DAOQWR010000000
VERSION     DAOQWR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13329
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5142_length_13329_cov_5.567952"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKK36_06330"
     CDS             complement(<1..592)
                     /locus_tag="PKK36_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR94212.1"
                     /translation="MQLTRQSPGFQPGINVLAEKHAGLLEGRRIGLVAHGASVDINGI
                     HTARLVQSRFGSGLKCIFGPEHGFLGTAAAGDAVDNARHPEWGIPIYSLYGETRKPTA
                     AMLADLDVLIVDLQDLAVRCYTYVSTLRLVMEAAAEHGIDVIVADRPVPFPNVCDGPM
                     LDPTFESFVGMISAPLIYGMTPAETSLFLKKQMMPDV"
     gene            complement(583..1353)
                     /locus_tag="PKK36_06335"
     CDS             complement(583..1353)
                     /locus_tag="PKK36_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNR94213.1"
                     /translation="MTKAAQSFSRVELLLGEEGFARLQASFVVVAGMGSVGSFAVESL
                     ARTGVGHLRLVDFDVVNRSNINRQLYALEATIGRKKVEIARERVMQINPACHVEALEL
                     FIDDTNVSRVLANKPAVVIDAIDSVNPKATLLAAAVEAGIPVVSAMGAATRTDPSCIH
                     VADIHKTHGCPLARMIRKRLGKRGIKHGIRCIYSTEKTSDDHRLDADDPETDSERTVD
                     RGRKRDVLGSICSIPGIFGLIAAREAVFQILKGTQPCN"
     gene            complement(1350..2126)
                     /locus_tag="PKK36_06340"
     CDS             complement(1350..2126)
                     /locus_tag="PKK36_06340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR94214.1"
                     /translation="MKLFDSHCHLQDERLAGRLDAVIERACRAGVRRIVCCGSSESDW
                     RRVAELADRYDFIHPAFGLHPWYTAGRSKDWLAELERFLTERPSMTGEIGLDHAVDER
                     NDEDQEAVFAVQMELAARLDRPVTIHCRRAWDRLPVLLKEMKTRPRFMIHSYSGGAEL
                     MGPLAELGAFFSFSGSITLNGNRKGIRAAAACPADRLLLETDAPDIPPLLPDGRRAEM
                     SEPASLTLVLKVAAAARGVSDVDLAAQVWENSCRFCDLET"
     gene            complement(2128..2832)
                     /locus_tag="PKK36_06345"
     CDS             complement(2128..2832)
                     /locus_tag="PKK36_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR94215.1"
                     /translation="MKPAGVVFFVLVLMGIPAPAQEESRIWTSVSGAKIEAALDSIRS
                     DMVVLNKSGGGQVRIRLNQLSSADQVYARSRTSKTPARINAKEPDAAVPSAIEELFGD
                     RLINAKKQSISPATLSGRKIGIYFSAHWCPPCRAFTPKLVEVYNQLKTDSKPFEIVFV
                     SSDREEQEMMNYMKEMDMPWLALRYGDKHIAKLKERFQVAGIPTLVIVDDKGNTISAN
                     ARGDVASKGAAAFDAW"
     gene            2978..3355
                     /locus_tag="PKK36_06350"
     CDS             2978..3355
                     /locus_tag="PKK36_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94216.1"
                     /translation="MAKIMIVDDEPDVRNMLESVLRSEGHQVLPVGDGLEALKILRGL
                     EEVDIMVTDLHMVPVDGLEIIETGHAERPDMDIIVLSAFINDDTFQRLVNLGVSGFVD
                     KPFTLEDLFVPIREILIKRSSKH"
     gene            3449..5167
                     /locus_tag="PKK36_06355"
     CDS             3449..5167
                     /locus_tag="PKK36_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR94217.1"
                     /translation="MTTAGTQKLAGLLEQTGLFTSAQVEDILQRQQQNGMSITDVVLD
                     MGYARDDEFLKALAKAMDIPFVRLAGTVIDASVLQKLPTKAVFQYNVVPVTMEKGALR
                     VATHNPFHPGLVDALRLASGFRIRLALSTADDLSKASKKFYGVGADTLDQMIQHDRIE
                     VASEEDLLPADLSDLDQEASVVKFVNQIIWEAHQDRATDIHMEPMETSLRIRYRVDGV
                     LHQTPVPAQLKRFQSAIISRIKVMSNMDIAEKRLPQDGRISLRIRGEEIDVRVSTMPT
                     VYGESVSLRLLMRSSGMLGMDKLGMDARDSKILTRMIHKPHGILLVTGPTGSGKSTSL
                     YAWLHTINSVDKRILTIEEPIEYEMEGVNQINVRPQIGLTFAMGLRHILRQDPDVIMV
                     GEIRDRETAEIAIRAALTGHLVFSTLHTNDAPGAVTRLLDMGIEPFLVASSVEGLVAQ
                     RLVRRLCPACRQPLPVDKASLANIGFPMERLSEGTLHTAAGCEECRMTGFRGRTGIYE
                     ILAVDDHIRPLIVARASSAAIKTEALRRGMHTLREDGWTKALQGVTTVEEVLRVSEED
                     ETLSEG"
     gene            5176..6423
                     /locus_tag="PKK36_06360"
     CDS             5176..6423
                     /locus_tag="PKK36_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR94218.1"
                     /translation="MPIYTYTARLASGDKKEGTLEANDRQSALIQIERMGAVPVSIRE
                     GTAAAAAPSGRGAKKRFALTLPQRAPRMNLRDVLLLTGEMSDLLASGMTLGHALNTLS
                     RRDTDPGQTAIVTALRDEIIQGSSLSDALRKWPKTFSPLYVSMVRAGEASGMLSEALS
                     RLRTHYERVLEAREKVSMALIYPAIILLLGGATLVFAVVFVVPRFTAIFAELGSTLPL
                     PTRILIRLSELMIDYGLVVLIAAVAAGILLRNALRTEKGRLVWHRMQLHLPVVRHIIT
                     ANAFGHFARTLGALLNNGVPVLQALGIVEETVGNVVIANEIHEARERVTDGSSISGPL
                     AAGKIFPPLLTDMLAVGEESGDMPGALAHIARRYDSDLDRSVKILTTLLEPVLMVGMA
                     VLVGFVAISMLLAVFDLTSGLNV"
     gene            6443..6841
                     /gene="gspG"
                     /locus_tag="PKK36_06365"
     CDS             6443..6841
                     /gene="gspG"
                     /locus_tag="PKK36_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNR94219.1"
                     /translation="MKKRKADYSSTAGFTLIEILLVVVIIGILAAVAVPRFAGRTGQA
                     KEGAAKASIKAIGVALDLYEMDHGSYPASLNALVENSGSSEWRGPYLKDGLPKDPWGN
                     DFIYSSQGASYDLKSGGEPGSGKEITGKND"
     gene            6847..7428
                     /locus_tag="PKK36_06370"
     CDS             6847..7428
                     /locus_tag="PKK36_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94220.1"
                     /translation="MTPGREHRECAENTAAFTLIEVLLVVVIVMITTGIAVPAFVNSY
                     RGVKMRTSVRGIVMASRYAHSVAVLNQMQTALFFDVEKGDYEIVALAGANALSQKERF
                     LEGRGAPAAAEGEEPQYSIEKRLSRELPDGIRIIAVESGQDAQQHEGIFWINFYPNGM
                     CDEFSVTLADDRDRTVTVGIDPLSSKPKVEYDR"
     gene            7418..7831
                     /locus_tag="PKK36_06375"
     CDS             7418..7831
                     /locus_tag="PKK36_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR94221.1"
                     /translation="MTADRRAGLSLVEVLMAILILGIGISSLVMAASRCLAVVRKAKN
                     FEIARRLLGEIELTEPLIPGEIEAGEESGNCESPYRDWRWTRTVEEDETDEESGLFKI
                     TMRVYWSEKGRETFEEVITYLYSPEDKGGGTVVSR"
     gene            7818..8537
                     /locus_tag="PKK36_06380"
     CDS             7818..8537
                     /locus_tag="PKK36_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR94222.1"
                     /translation="MSHADQRGFTLLEVLCAVAILATAMSVILGTFTVCVRAWTRGEE
                     ALDDLHHGDYVMEQLVSALRSAAWFGTSPGKYGFHLEDRGGDYPQDMISWVTSGTAFL
                     PQGSELAHGLHRLTVSIEDNEDGDDAVAIRSFPHLAELKDDESEPWFVSSVVKGLNCR
                     IYNVEDEDWEDDWEDTNAIPQIVEITLYMDPLERYGDPITIKRMVQIPVAGAVEKRVL
                     TTQPAGQPPDGAMPRTRKIVR"
     gene            8705..9649
                     /locus_tag="PKK36_06385"
     CDS             8705..9649
                     /locus_tag="PKK36_06385"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNR94223.1"
                     /translation="MIGTFAFNMHIEAGITSHYRKRVKAQYLARAGGEWAQYLLAQTG
                     SDLDEETEDSPLEEDVLTQAKRILRGNAVYSLKQELGAGTFSIDIIPEEGRGNINQMT
                     DEQWEEMLDQAGVPEEQWEELIDCFADWIDENDEHHLNGAESDDPFYKERGYECKNAP
                     LDTVDELLLIKGFTAAIVYGGPAEREDDPPYGGIASMLTVWGDGKININTATRETMLA
                     FLPGIDEWMVDDIIEGRKGLDGEENTDDDGFESVDEAIAKAGLPADLKGKITTVEKKY
                     VRVVSMGEVGAVKSGIWAVMRYDGGKVVPVFWREEMMQ"
     gene            9741..11141
                     /gene="pilM"
                     /locus_tag="PKK36_06390"
     CDS             9741..11141
                     /gene="pilM"
                     /locus_tag="PKK36_06390"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNR94224.1"
                     /translation="MASSLITAVRWKGTSLDVTSVRPRKTGPAELAGHRIIPLETEGA
                     LPETGTPELSALLKKACHGVQGRICLAIPTDQALLRVMDLPAAEESELQGMVELQVDR
                     VSPFPLETMAVNFEVLHRNTNSFRVLIAAVRRDIIEQNASTFDKAGLLPHWMDVELPG
                     WLYLLREAGQIAPEGRQVILLVTGKTCEMIVIHNGLPVMLRFLGLHGGLGSPEEIDEI
                     ADEMSYTLTALETEWGAVEASRLSIWSSEPPPPALMQALKTQTGLDAETHLLEELSPL
                     SEGLARRAAEDRESAHLNLVPPEWHQKEAARRVQKKLLLATGVFFLVWGLAMAAFHAG
                     MTMQQKKLSGLEARAGRIKKEAAEVREVQREVRGLELYGSRSHSALECLREVILLMPE
                     NMELTVFNYKKAGTVFLTGQIPGGADPIYAFKAALDKSELFEEIELGLTPTKTLRDGS
                     KFSEFKITVNLPGGDS"
     gene            11138..11692
                     /locus_tag="PKK36_06395"
     CDS             11138..11692
                     /locus_tag="PKK36_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94225.1"
                     /translation="MRISTRELMLAWLTLVVVVLALSWYTGRRKWDAFQQAREQSSGL
                     AARMEHDERLLDERAALMQRLQTLRDQLPSHPAKLDVTSELLRSLEQTANKNGLVLKT
                     RSAGNESVSSNQLYEISIKCDWEGTLETLVPFLFDIQEQGVRYDIRELRVVPSRGEKE
                     RLNGNFILDCAYSRQPAGNASEGL"
     gene            11704..>13329
                     /locus_tag="PKK36_06400"
     CDS             11704..>13329
                     /locus_tag="PKK36_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNR94226.1"
                     /translation="MHKRWARGGLSWMIFLLLAGSLSAPAQTLDVRPSAPARSSKKIT
                     LNYKDSPLDMVLDDYSEWTGRTLIKSPSVPKVTITLRSFTELTVDEALVALDSVLSMH
                     NITLVPMGDKFFRVVATANARTEGMPLGVAENDEEFADTDKLITRLIRLQHIPPAEAQ
                     PLLQSILHGYGKMQTMEQINSILITDTSANLLRIQEVLKLIDLPIELKIQHRIYTIQH
                     AKASDIASKLNEIIQTSQETQGGEAAAAKSVPTRAAPPGVIRARSPAEEAGATAADVT
                     RALAERGIAQGRVKIISDERTNIMIIISDPVNFKFFDEIVAILDVPTDPEITVKVVKL
                     EYAEAEEISGTLNEFIGVAKAEESATAGGTAAAGQPGAAQGQTLRDYAIQRAQARAAA
                     VSAEEKAKIGQLSPDTKILADKRTNSLLLMGRRADIAALEDIIAELDVMLAQVIIETV
                     ILEVSLSENLTYGVDWLQKSMTVVNENRQGPRGGIRVTEPVMSFGGGWATTDREVNSF
                     KDGSRIQRDSVALTGGSLSYYLTLFDLNVDAIIR"
BASE COUNT         2671 a         3927 c         4232 g         2499 t
ORIGIN      
        1 aaacatcggg catcatttgc tttttcagga acagcgaggt ctcggcgggt gtcatgccgt
       61 agatcagcgg agcggagatc atgccgacaa aactttcaaa agtgggatcc agcattggcc
      121 cgtcgcacac attggggaag ggaaccggcc ggtccgcgac gatgacatca atgccgtgtt
      181 cggcggcggc ttccatgacg aggcgaagcg tggagacata ggtgtagcag cgcacggcga
      241 gatcctgcag atccacgatg agcacatcca gatccgccag cattgccgct gtcggcttgc
      301 gggtttctcc gtaaagggaa taaattggaa ttccccattc gggatgacgg gcgttatcca
      361 cggcatctcc ggcggccgct gttcccagaa aaccgtgctc gggcccgaag atgcatttga
      421 gtccgctccc gaacctgctt tgcacaagtc gtgcggtatg aattccgttg atgtccacac
      481 tggccccatg ggccaccagc ccgatccgcc ggccttccag caggccggca tgcttttctg
      541 ccagcacatt gatgcccggc tggaaaccgg gagattgccg ggtcagttgc atggctgggt
      601 ccccttcaga atttgaaaga cggcttcgcg ggcggcgatg agcccgaaga ttccgggaat
      661 gctgcagatg ctcccgagga catcgcgctt ccggccgcgg tcaacggtcc gctcgctgtc
      721 ggtttcggga tcatcggcat ccagccggtg gtcgtccgag gttttttcgg tggaatagat
      781 gcagcggatt ccatgcttga ttccccgctt ccccagtcgt ttccggatca tgcgggcaag
      841 ggggcagcca tgggtcttgt gaatatcggc cacatgaatg caggagggat cggtgcgcgt
      901 ggccgcgccc atcgcggaaa cgacagggat tccggcctca accgcggcgg ccagcagcgt
      961 cgccttggga ttcacgctgt ctatcgcgtc aatcacgaca gccggtttgt tcgccagcac
     1021 ccggctgacg ttcgtatcgt caatgaacag ttccagcgct tcgacatggc aggcgggatt
     1081 gatctgcatc acccgctcgc gggcgatttc gactttcttg cggccgatgg tggcttccag
     1141 cgcatagagc tggcggttga tgttgctgcg gttgacgaca tcgaaatcca caagccggag
     1201 atgtccaacg cctgtgcggg caaggctttc gacggcgaac gaccccacgc tgcccattcc
     1261 ggccacaacg acgaaggatg cctgaagcct ggcgaatccc tcttcgccca gcaggagttc
     1321 aacgcgtgaa aatgattgtg ccgcctttgt catgtttcga gatcgcagaa gcggcaggag
     1381 ttctcccaca cctgggcggc caaatcaaca tcggaaacgc cgcgggcggc ggccgccact
     1441 ttcagcacaa gcgtcaggct ggcgggttcg ctcatttcgg cgcgcctgcc gtcgggcagg
     1501 agcgggggga tgtcgggggc gtcggtttca agcagaagcc ggtcggcggg gcaggcggcc
     1561 gccgcccgga tgcctttccg gttgccgttc agtgtgatcg aaccggaaaa agaaaaaaat
     1621 gcgccaagtt ccgccagcgg gcccatgagt tcggcgccgc cggaatagga atgaatcatg
     1681 aaccggggac gggtcttcat ttctttcagc agaaccggca gccggtccca tgcgcgccgg
     1741 cagtggatgg tgacgggccg gtccagccgt gcggccagtt ccatttgaac ggcaaagacc
     1801 gcttcctgat cctcgtcatt gcgctcatcc accgcgtgat ccagtccgat ctcgccggtc
     1861 atcgaaggcc tttccgtcag gaaccgttcg agttccgcca gccagtcctt ggatcttccc
     1921 gcagtatacc acggatgcag gccaaaggcg ggatggatga agtcgtatct gtctgccagt
     1981 tcagcaacgc ggcgccaatc gctctcggaa ctcccgcagc agacgatgcg ccggacaccg
     2041 gccctgcagg cgcgttcgat tacggcatcc agtctgccgg ccagccgttc atcctgcaaa
     2101 tgacaatggg aatcaaagag cttcatgtca ccatgcatcg aaggcggctg cccctttgct
     2161 cgcaacgtcc cccctcgcat tggcggaaat ggtgttgccc ttgtcgtcca caatcaccag
     2221 tgtgggaatg cccgcaacct ggaagcgctc cttcagcttg gcgatgtgct tgtcgccgta
     2281 ccggagagcc agccagggca tgtccatctc cttcatgtag ttcatcattt cctgctcttc
     2341 ccggtcgctg ctgacaaaaa caatttcgaa cggtttgctg tcggtcttga gctgattgta
     2401 gacctcgacc agtttggggg tgaaggcgcg gcagggcggg caccagtggg cggaaaaata
     2461 gatgccgatc tttctgcctg acagcgtggc cggggagata ctctgctttt ttgcgttgat
     2521 cagccggtcg ccaaacagtt cctcaatggc cgaagggacc gcagcgtccg gctcttttgc
     2581 gtttatccgc gccggggttt ttgaggtccg gcttcgggcg tacacctgat ccgcgctgct
     2641 gagctgattc aggcggattc tcacctgtcc tccgccgctt ttgttaagaa ccaccatgtc
     2701 gcttcgaatg gaatcgaggg cggcttcgat tttggcgccg ctgacggagg tccagatgcg
     2761 ggactcctcc tgggccgggg cgggaatccc catcagcaca agcacaaaga aaaccacacc
     2821 ggccggtttc atagttcagt ctccttataa atgtgcagat tttaggcgcg ggaaagcgaa
     2881 aaaacaaacc aaatccgccg ggcagggcct aaaacagagc ttgtaggata cctgctgaac
     2941 cgcttaaatc tgactgttca atcctgagtg gggctttatg gcaaaaatta tgatagtgga
     3001 cgatgagccg gacgtgcgca atatgctcga aagcgtgctg cgttcggagg gccatcaggt
     3061 tcttccggtc ggggacggcc tcgaggctct gaaaatcctc cgcggcctgg aggaagtgga
     3121 catcatggtg accgatctgc acatggtgcc ggtggacggg ctggaaatca tcgaaaccgg
     3181 ccatgccgag cgcccggaca tggatattat tgtgctttcc gcctttatta atgacgatac
     3241 ctttcagaga ctggtgaatc tgggcgtcag cggttttgtg gacaaaccct tcacgctgga
     3301 ggacctgttt gttccgatcc gcgagatcct gatcaagcgc agcagcaagc actgatggcg
     3361 ctgattctga acagcggccg ggattttcgg tcaaacaggt gtataaacaa tttgccgggc
     3421 tttgtcccgg ccgcagagga atgagcgcat gacaaccgca ggaacacaga aactggccgg
     3481 actgctggag cagaccgggc ttttcacgtc cgcgcaggtg gaggatatcc ttcagcgcca
     3541 gcagcagaac ggcatgtcca tcaccgatgt ggttctcgat atggggtatg cccgggacga
     3601 cgagttcctg aaggcgctgg cgaaggccat ggatattccc tttgtgcgcc tcgccggcac
     3661 cgtgattgac gcctccgtgc tccagaaact cccgaccaag gcggtttttc agtacaatgt
     3721 ggtgcccgtc accatggaaa agggggccct gcgcgtggcc acccacaatc cctttcatcc
     3781 ggggctcgtc gatgcgctgc gcctcgcatc cggtttccgg atccggctgg cgctgagcac
     3841 ggcggacgat ctcagcaagg catccaagaa gttctacggc gtcggggcgg acaccctcga
     3901 ccagatgatt cagcacgacc gcattgaggt ggcgtcagag gaggacctgc tgccggccga
     3961 tctcagcgat ctggaccagg aggcttcggt tgtcaagttc gtcaaccaga tcatctggga
     4021 agcgcatcag gaccgcgcaa cggacatcca tatggagccg atggaaacct ccctgcgcat
     4081 ccgctaccgc gtggacggag tgcttcatca aacgccggtc cccgcgcagc tcaagcgttt
     4141 ccagtcggcc attatctccc gcatcaaggt catgagcaac atggacattg ccgagaagcg
     4201 cctcccgcag gacggccgca tcagcctgcg cattcgaggc gaggagattg acgtgcgcgt
     4261 gtccaccatg ccgacggtgt acggcgaaag cgtgagtctt cgtcttctga tgcgttccag
     4321 cggcatgctg ggcatggaca agctgggcat ggatgcgcgc gactcgaaga tcctgaccag
     4381 aatgattcac aagccgcacg gcatcctgct ggtgaccggc ccgacaggct ccgggaaatc
     4441 cacctctctc tatgcgtggc ttcacaccat caattccgtg gacaagcgca tcctgaccat
     4501 tgaggaaccg atcgaatacg agatggaggg cgtgaaccag atcaatgtcc gcccgcagat
     4561 aggcctgacc tttgcgatgg ggcttcgtca cattctgcgc caggatcccg acgtgatcat
     4621 ggtgggggaa atccgcgacc gcgagactgc ggaaatcgcc atccgcgccg cgctcacggg
     4681 gcatcttgtt ttcagcacgc tgcacaccaa cgatgccccc ggtgccgtca cgcgcctgct
     4741 ggacatgggg atcgaaccct ttctggtggc ctcgtcggtc gaggggctgg tggcccagcg
     4801 cctggtgcgg cgcctgtgtc ccgcctgccg ccagccgctg ccggtggaca aggcctccct
     4861 ggcgaacatc gggtttccga tggagcggct cagcgagggc acgcttcata cagccgccgg
     4921 ctgcgaggaa tgccgcatga ccggcttccg cggacggacc ggcatctatg agattctcgc
     4981 ggtggacgac cacatccgcc cgctgattgt ggcgcgcgcc tcctcggcgg ccatcaagac
     5041 cgaggcgctc cgtcgcggaa tgcacacgct gcgcgaggac ggctggacca aagccctgca
     5101 gggcgtgacc acggttgagg aagtcctgcg cgtctcggaa gaagacgaaa cactctctga
     5161 aggataactg ccgtcatgcc gatctacacc tataccgccc ggctggccag cggtgacaaa
     5221 aaggaaggca cgctggaagc gaacgaccgc cagtccgcgc tgatccagat cgagcgcatg
     5281 ggggcggtcc cggtttccat tcgcgagggg actgcggccg ccgccgcgcc gtccggccgg
     5341 ggcgcgaaaa agcgctttgc gctgaccctg ccgcagcgcg ctccgcgcat gaacctgcgc
     5401 gatgtgcttc tgctgacggg ggagatgagc gacctgctgg cgtccggcat gacactgggc
     5461 catgcgctca acacgctctc ccgccgcgac acggatcccg gccagacggc cattgtcacc
     5521 gcgctccgcg acgaaatcat ccaggggtcc agcctctcgg acgcgcttcg caaatggcca
     5581 aaaacgtttt ctcccctcta tgtcagcatg gtccgcgcgg gcgaggccag cggcatgctt
     5641 tcggaggccc tttcgcggct gcgcacgcat tacgagcgtg tgctggaggc ccgcgaaaaa
     5701 gtatccatgg ccctgattta tccggccatt attctgctgc tgggcggagc cacgctggtg
     5761 ttcgccgttg tcttcgtggt gccgcgcttc acggccatct ttgccgagct gggaagcacg
     5821 ctcccgctgc cgacgcgcat cctgatccgg ctgagcgaac tcatgattga ctacgggctg
     5881 gtggtcctga tcgcggccgt ggcggcgggc atcctgctcc gcaatgcgct tcgcaccgag
     5941 aaggggagac tggtttggca ccgcatgcag ctgcatcttc ccgtggtccg gcacatcatc
     6001 acggccaacg ccttcggtca tttcgcccgc acgctgggcg cgctgctgaa caacggcgtg
     6061 ccggtgctgc aggcgctggg cattgtcgag gaaacggtcg ggaatgtcgt cattgccaac
     6121 gaaatccacg aggcccgcga gcgggtgacc gacggttcca gcatttctgg cccgctggcc
     6181 gcgggaaaaa tctttcctcc gcttttgacc gacatgctgg ccgtgggcga ggaatccggc
     6241 gacatgccgg gggcgctggc gcatatcgcc cgccgctacg attccgatct cgaccggagc
     6301 gtcaagatcc tgaccacgct gcttgagccg gtgctgatgg tgggcatggc cgtgctggtc
     6361 ggatttgtgg caatttcgat gctgctggcg gtttttgacc tgaccagcgg cctgaatgtc
     6421 tgattgcgga aggagaagca aaatgaaaaa gagaaaagca gattacagct ccacggcggg
     6481 tttcaccctg attgaaattc tgctggtggt ggtgattatc ggcatccttg cggcggtggc
     6541 ggtaccgcgc tttgccggcc gcacgggcca ggccaaagag ggcgccgcga aggcgagcat
     6601 caaggccatc ggcgttgcgc tcgacctgta tgaaatggat cacgggagct atccggccag
     6661 cctgaacgcg ctggtggaaa acagcggaag ctccgagtgg cgcggcccct acctgaagga
     6721 cggcctgccg aaggatccct ggggcaatga tttcatctac tcttcgcagg gggcctccta
     6781 tgacctgaag tccggcggcg agcccggcag cggcaaggaa atcaccggca agaatgacta
     6841 gcgcggatga ctcccggacg tgaacaccgc gaatgcgcag aaaacacggc agccttcacg
     6901 ctgattgaag tcctgctggt ggtggtgatt gtcatgatca ccaccggaat cgcggtgccg
     6961 gcgttcgtga actcctatcg cggcgtgaaa atgcgcacct cggtgcgggg cattgtgatg
     7021 gcctcccgct acgcgcactc cgtggcggtc ctcaaccaga tgcagaccgc gcttttcttc
     7081 gacgtggaaa aaggagacta cgaaatcgtc gcgctggccg gcgccaatgc cctcagccaa
     7141 aaggagcgtt tccttgaagg ccgcggcgcg cccgcggccg cggaaggcga agagccgcag
     7201 tattccatcg agaagcgcct ttcccgggaa ctgccggacg gcatccggat catcgcggtt
     7261 gagagcggtc aggatgcgca gcagcacgaa ggcattttct ggatcaattt ttatccgaac
     7321 ggcatgtgcg atgagttttc cgtgacgctg gcggacgacc gcgaccgtac cgtgacggtc
     7381 gggattgacc ccctttcctc gaaaccgaag gtggaatatg accgctgacc gcagagcggg
     7441 gctgagcctc gtcgaggtgc tgatggccat cctgatcctc gggatcggga tttcttcgct
     7501 ggtcatggcg gcctcgcgct gcctggcggt tgtgcgcaag gcaaagaact ttgaaattgc
     7561 gcggaggctg ctgggcgaaa tcgagctgac cgagccgctg atccccggcg aaatcgaggc
     7621 gggcgaggaa tccgggaact gcgaatcgcc ctaccgcgac tggcgctgga cgcgcacggt
     7681 cgaggaggac gagaccgacg aggagtccgg cctcttcaaa atcaccatgc gtgtttactg
     7741 gtcggaaaag ggccgcgaaa cctttgaaga ggtgatcacc tatctctatt cacccgagga
     7801 caagggagga ggcaccgttg tctcacgctg accagcgcgg cttcacgctg cttgaagtgc
     7861 tctgcgcggt ggcgatcctt gccaccgcga tgagcgtgat tctcggcacc ttcacggtct
     7921 gtgtgcgcgc atggacgcgg ggggaggagg cgctggatga cctgcatcac ggcgactacg
     7981 tcatggagca gctggtttct gcgctgcggt cggcggcgtg gttcggcacc tcgccgggca
     8041 agtacgggtt tcatctcgag gaccgcggcg gggattatcc gcaggacatg atcagctggg
     8101 taacctccgg cacggccttc ctgccgcagg gttccgaact cgcgcacggg ctgcaccgcc
     8161 tgacagtcag catcgaggac aacgaggacg gcgacgatgc cgtcgccatc cgctcgtttc
     8221 cgcatctggc ggagctcaag gacgacgaat ccgaaccgtg gttcgtttca tccgtggtca
     8281 agggcctcaa ctgccggatc tacaacgtgg aggacgagga ctgggaggac gactgggagg
     8341 acaccaacgc gataccgcag atcgttgaga tcaccctcta catggatccg ctcgagcgct
     8401 atggagaccc catcaccatc aaacgcatgg ttcagattcc ggtggcgggc gccgtggaaa
     8461 aacgggtgct gaccacacag ccggccgggc agccgccgga tggagccatg ccgcgaacga
     8521 ggaaaattgt ccggtgagcg gaaaacttcc aaccattgga acttttgcgg gaaaaacttc
     8581 caatcattgg aactttttgc ggaaaaactt ccaatcattg gaacttttac aaaattgtaa
     8641 aagttccggc cggtccggct cggccctgat tgtcgcgctt tggacgcttt tgattttgac
     8701 gctgatgatc gggacgttcg cgttcaacat gcacatcgag gcgggcatca cgtcgcatta
     8761 tcgcaagcgg gtcaaggcgc agtacctggc gcgggccggc ggcgagtggg cgcagtacct
     8821 gctggcgcag acgggatcgg acctggacga ggagacggag gattctccgc tcgaggagga
     8881 tgtgctcacg caggccaaac gcattctgcg gggcaatgcc gtctacagcc tgaagcagga
     8941 actgggtgcc ggcaccttca gcattgacat cattcccgaa gagggccgcg ggaacatcaa
     9001 ccagatgacg gacgagcagt gggaggagat gctggatcag gccggcgtgc ccgaggagca
     9061 gtgggaagaa ctcattgact gctttgccga ctggattgat gagaacgacg agcaccacct
     9121 caacggcgcc gagtcggacg acccgttcta caaggagcgc ggctacgagt gcaaaaacgc
     9181 gccgctggac acggtggatg aactgctgct gatcaagggg ttcacggccg ccattgttta
     9241 cggcggcccg gcggagcggg aggatgaccc gccctatggg gggattgcct ccatgctgac
     9301 ggtctggggc gacggcaaga tcaacatcaa cacagccacc cgtgagacga tgctggcctt
     9361 tctcccgggc attgatgagt ggatggtgga tgatatcatc gagggccgaa agggactgga
     9421 cggggaggag aacacggatg atgacggctt tgagagcgtg gatgaggcca ttgccaaggc
     9481 cgggctgccg gcggacctga agggcaaaat cacgacggtg gaaaagaagt atgtgcgcgt
     9541 ggtgtccatg ggcgaggtgg gcgcggtcaa aagcgggatt tgggcggtga tgcgttacga
     9601 cggggggaag gttgtgcccg tgttctggcg cgaggaaatg atgcagtgac gcgcaaaaaa
     9661 tgaacgccgg agcataaaaa aggttcgaaa gcaggcggga agtgtataaa ctgccccgca
     9721 aaaacagata ataaacggaa atggcatctt ctctgatcac agcagtcagg tggaagggaa
     9781 cgtcactgga tgtgacttcc gtccgcccgc gcaagaccgg acccgccgaa ctggccgggc
     9841 accggattat tccgcttgaa accgaaggcg ctcttcccga gacgggcaca ccggaactct
     9901 cggcgcttct gaagaaggcc tgtcatggag tgcagggcag gatatgcctc gcgattccca
     9961 ccgatcaggc gctgttaagg gtcatggacc tgcccgccgc cgaggaaagt gaactgcagg
    10021 gcatggttga actgcaggtg gatcgcgttt cgcctttccc gctcgagacg atggcggtga
    10081 attttgaggt cctgcaccgg aacacgaatt ctttccgcgt gctgatcgcc gccgtgcggc
    10141 gggatatcat cgagcagaac gcctcgactt ttgacaaggc cggcctgctt ccccactgga
    10201 tggatgtgga actgcccggc tggctttacc tgctcaggga ggccgggcag atcgcgccgg
    10261 aaggccggca ggtgatcctg ctggtgaccg gcaagacctg tgagatgatt gtcatccaca
    10321 acggccttcc ggtgatgctc cgcttcctcg gcctgcatgg cgggctggga agccccgagg
    10381 aaattgatga gatcgccgat gaaatgtcct acacgcttac cgcgctggaa accgaatggg
    10441 gcgccgtcga agcctcgcgg ctgagcatct ggtcgtccga gccgcctccg ccggccctga
    10501 tgcaggccct gaagacccag accggcctgg atgccgagac gcacctgctg gaggaactgt
    10561 cgcccctgag cgaagggctg gcgcgccgcg cggcggaaga ccgcgagagt gcccacctga
    10621 atctggttcc cccggaatgg catcagaagg aggcggcccg ccgcgtgcag aagaagctgc
    10681 tgctggccac cggtgtgttc ttcctggtct ggggtttggc gatggctgcc tttcatgcgg
    10741 gcatgacgat gcagcagaag aagctgtcgg gactggaggc ccgagcgggc cggatcaaga
    10801 aagaggccgc cgaagtgcgc gaggtgcagc gcgaggtgcg gggccttgaa ctgtatggaa
    10861 gccgaagcca ctccgccctc gaatgcctcc gcgaagtgat cctgctgatg ccggagaaca
    10921 tggagctcac ggttttcaac tacaagaagg cgggcacagt attcctgaca ggccagatcc
    10981 cgggcggcgc ggatccgatc tacgccttca aggccgcgct cgacaagtcg gagcttttcg
    11041 aggaaatcga actgggcctg actccgacca agacgctgcg tgacggaagc aagttttcgg
    11101 aattcaagat cacggtgaac cttccgggag gggactcatg aggatatcga cccgggagct
    11161 gatgctggcg tggctgaccc tcgtggtggt cgtgctggcc ctgagctggt acaccggccg
    11221 gcgcaaatgg gacgctttcc agcaggcgcg tgagcagtct tcagggcttg cggcccgcat
    11281 ggagcatgat gagcggctgc tggatgagcg cgccgcgctg atgcagcgcc tgcagaccct
    11341 gcgcgatcag cttccatctc atccggcgaa gctggatgtc acctcggaac tgctgcgcag
    11401 tctggagcag accgcgaaca agaacggcct tgtgctcaaa acccgctcgg ccggcaacga
    11461 atccgtcagc agcaatcagc tttacgagat ttccatcaaa tgcgactggg agggcacgct
    11521 ggaaaccctg gtgccgtttc tttttgatat acaggaacag ggtgtgcgtt atgatatccg
    11581 cgagctccgg gttgtcccct cccgggggga aaaggaaagg ctgaacggga atttcatact
    11641 ggattgcgcc tacagccgcc agccggcggg gaatgcgagt gaaggcttat gatgctctat
    11701 aaaatgcata aacggtgggc ccgcggcggg ctttcctgga tgatattcct tctgctggcc
    11761 ggaagtcttt ctgcccccgc ccagacgctg gatgtccgcc cctcggcgcc cgcccgttcg
    11821 tccaagaaaa tcacgctcaa ctacaaggat tcgccgctgg acatggtcct ggacgactac
    11881 agcgagtgga ccggccgcac cctgatcaag tcgccttccg ttcccaaggt gaccatcacg
    11941 cttcggagct tcaccgagct gaccgtggac gaggcccttg tggcgctcga cagcgtgctt
    12001 tccatgcaca acatcaccct cgtgcccatg ggggacaagt tcttccgcgt ggtggccacc
    12061 gccaacgccc ggaccgaggg catgccgctg ggcgtggcgg agaatgacga ggagttcgcg
    12121 gacacggaca aactgattac gcgcctgatc cgcctgcagc atattccccc cgcggaggcc
    12181 cagccgctgc tgcagagcat tctgcacggc tacgggaaga tgcagaccat ggagcagatc
    12241 aattccattc tgatcacgga cacttccgcg aacctgcttc gcatccagga agtgctgaag
    12301 cttattgatc tgcccatcga gctgaagatc cagcaccgca tttataccat tcagcacgca
    12361 aaggcctcgg atattgcatc gaagctgaat gaaatcattc agacgtcgca ggaaacccag
    12421 ggaggagagg ccgctgctgc aaaatccgtg cctacgcgcg ctgccccgcc cggagtgatc
    12481 cgtgcgcgct cgcctgcgga ggaagccgga gccaccgccg ccgatgtcac ccgcgcgctt
    12541 gccgagcgcg gcatcgcgca gggccgtgta aagatcattt ccgatgagcg gaccaacatc
    12601 atgatcatca tttccgaccc ggtgaacttc aagttcttcg atgagatcgt ggccattctg
    12661 gatgtgccga ccgatcccga gatcaccgtg aaggtggtca aactggaata tgccgaggcc
    12721 gaggagatat ccggaaccct gaatgagttt atcggcgtgg ccaaggccga ggagagcgcg
    12781 accgcgggcg gcacggccgc agccggccag ccgggcgcgg cccagggcca gacgctccgc
    12841 gactacgcga tccagcgcgc gcaggcccgc gcggccgcgg tgtccgccga agagaaggcc
    12901 aagatcggtc agctctcgcc ggacacaaag atccttgccg acaagcgaac caactcgctt
    12961 ctcctgatgg gccgccgcgc ggatatcgcc gcgctggagg acatcattgc cgagctggat
    13021 gtcatgctcg ctcaggtcat catcgaaact gtcattctgg aggtcagtct cagcgaaaac
    13081 ctcacctacg gcgtggactg gctccagaag tccatgacgg tggtgaacga gaacaggcag
    13141 gggcctcgcg gcggaatccg ggtgaccgag ccggtgatgt ccttcggcgg aggctgggcg
    13201 acaacggacc gcgaagtcaa ttcgttcaag gacggcagcc ggattcagcg cgacagcgtt
    13261 gcactgaccg ggggttcgct gagctattac ctgacgctct tcgatctgaa tgtggacgcg
    13321 atcatccgc
//
LOCUS       DAOQWR010000062        13019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5283_length_13019_cov_4.555076, whole genome shotgun sequence.
ACCESSION   DAOQWR010000062 DAOQWR010000000
VERSION     DAOQWR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_13019_cov_4.555076"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKK36_06405"
     CDS             <1..392
                     /locus_tag="PKK36_06405"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNR94227.1"
                     /translation="LNPAEALCVRRNLGVLRDLGFGISEFGGQTYMVDAMPACLGAVS
                     AAPILRDMLSHMAAGGTSGAAKWSREQLAQAACSAAVNARVKLTLAETEKLVVDLARA
                     RMPYTSPQGRPTLIFMSFSELRRKFGR"
     gene            complement(393..950)
                     /locus_tag="PKK36_06410"
     CDS             complement(393..950)
                     /locus_tag="PKK36_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94228.1"
                     /translation="MSKTHFQLNRPIHLAQLTVYFEKAHDLISLPLDRIGRDRLVQHI
                     ENITRLVTAASRNATLAARAPVAIDKEKDFNHLLMLIGHDLQALRALLTHEHQVEQEE
                     GFLFRFLQQEDRAARPTAVYNQRVEESIADIRQVICMACKPFEELKTALLAAMDETEK
                     ERYERACRCFREDLVPEIETNGLSS"
     gene            1165..3384
                     /gene="priA"
                     /locus_tag="PKK36_06415"
     CDS             1165..3384
                     /gene="priA"
                     /locus_tag="PKK36_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNR94229.1"
                     /translation="MSSYARVVVEIALNREFDYIIPEALRDAVAVGSQVVVPFGKSEV
                     RGFVVALPERSDRNDLREIIRLVGKKPLISGRVMELARWVAEYYAAPVETAVRTVLPS
                     AVRQKNARFKEQLFVIPMDAASDAEAVRRLRARSPRQAAVLDILLSGEAMFLSHLAKT
                     AGTTAGTVRGLEKKGFVRISREAMARDPLAGQTVLRTQHQKLMPQQEEAFALIRRAMD
                     TLAPPVVLLYGVTGSGKTEVYLQAIDHALKQGKGAIILVPEISLTPQTVERFQSRFGD
                     DIAVLHSHLSGGERHDEWHRLHSGKARIAIGARSALFAPIENLGLIVVDEEHEHSYKQ
                     GEAPRYHARDVAVMRGHMERCAVVLGTATPSLESWHNAQTGKYALARMPHRVDHRKMP
                     HVRVIDMRLEASKTGRVTMFSSELVEAVRARLARAEQVILFLNRRGHSTSLICPKCGY
                     VATCSACSVPLTYHKVTEELRCHICGAIEPVPDRCPSEECRDPRIRYAGIGTQKVELA
                     AAKLFPKANIQRMDADVTTQKDAYSRILGDFRVGKTDILIGTQMIAKGLHFPNVTLVG
                     VIAADVALHMPDFRAGERTFQLLTQVAGRAGRGDVSGEVIVQTFTPFHPSIQASSRID
                     FEGFADQEMEFRRELFYPPYSHLICITVRGRVEEQVKFSIDAFAKTLFPMLPEKVVRG
                     EPAPAPLARTCGQYRWQIMLRAASARAVTGPLRTALDQFRWPKEVHCTVDVDAISLM"
     gene            complement(3388..4884)
                     /locus_tag="PKK36_06420"
     CDS             complement(3388..4884)
                     /locus_tag="PKK36_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94230.1"
                     /translation="MKTLSVILAAAAMILTCRAQDARPEFRPPEVRRPETDGLPAGAN
                     RLEAEGEVESNSVTLVSGPDRLITASYKSLLEDGGYIITTVPYTGLRAAVAERTRLIV
                     LACAHMPPDSDAFIEFLLSVKNSDVPVIAVGDSARTILRQLELSMVQHATMVPESRVI
                     PITPLHSTLKKTRPIPISSNGFDLYSKMQTGTAVMLQSVPSYLRPIVQSASRPGAYPV
                     LLEDNRFLFWGYSGSPDIMSAQGRDLFLNLVDYLVQGKKEESGPHPHPRTEPGVNVVL
                     PARTAANQSSTEVITQQGGMTIHSTGSYPSKPVIYESKPPAADPSKDFKSCRLYFTSQ
                     ESRAGSAFYSFDAWLGIRSDQYRVVRDGSPCLRYQQEDGVLYHTPRIKISARNKAIPP
                     QSLLTVEYYSRSAVNDDLKLEAVQHIPISGIARGKSVMVDAGDIGTYSSSLKYDGYKR
                     KTGKEMYGIIVTLFDPGGTLLFQQTTQQQLARMASPKPKPPMIQESQR"
     gene            complement(4931..5788)
                     /locus_tag="PKK36_06425"
     CDS             complement(4931..5788)
                     /locus_tag="PKK36_06425"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNR94231.1"
                     /translation="MNALETSGIKKPRSGSDYALHHTALNGAERRGVIAAIDTIGQSA
                     TGPMKKFISAFDRELGEIISNGRAGTLDELDAAVTELLLRSNSALIPLNRDRRDIFGF
                     CITLCLMHGSECRVMQLGDCRAYLLEHRPDRAPAATMLTRDQNSLAMVMAVEKEYTFL
                     SNELLELSRTLHGYWGKPDTENVRAALENSKRTVELPEPCCVFAATDGIFLPLIRGLL
                     DLNNYRLTEEQFSLEAWLVNFLDTGGHLLHPGQWQHLVPDMIHLAEQYSAKKPRYRDD
                     IAAATAWIE"
     gene            complement(5792..6259)
                     /locus_tag="PKK36_06430"
     CDS             complement(5792..6259)
                     /locus_tag="PKK36_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNR94232.1"
                     /translation="MDIISLTVVSAAFALGDAMPAQYTCAGANVSPPLSWGAVPAGTK
                     SIAVLSDDPDCPGGWVHWVLINLPPEARELPEDVEPDPELPNGSIQGITDFRRPGYGG
                     PCPPPGRPHRYFFRVYALDKKLDLSPRAEMKDVQKAMAGHILAQGETMGRFSR"
     gene            6394..7296
                     /locus_tag="PKK36_06435"
     CDS             6394..7296
                     /locus_tag="PKK36_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR94233.1"
                     /translation="MKMICGILAAAMLLARPVPAAEAAERTVVLDGEFYLDEDIVFSK
                     SGRTMDILHPVETSGPLPAIVWIHGGGWGAGDKRRGARNILPFAERGYVCVSISYRLT
                     DEAVFPAQIHDCKAAIRYLRAHAGKYGIDPDRIGVWGASAGGHLAALLGTSGGITGLE
                     GDEGWPDFSSRVQAVCDWYGPVDFYSAAHQAPEDRRPEKLLGGEVLENRELAALASPV
                     SHATPDDPPFLIMHGSRDDVVPMKQSRLLKWALSRQGVPVYFSVVRGGEHGGERFEQE
                     KVRAKVMKFFDAHLKGPGPAQALP"
     gene            7350..8777
                     /locus_tag="PKK36_06440"
     CDS             7350..8777
                     /locus_tag="PKK36_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94234.1"
                     /translation="MKWYFQSGILLCALLLAGCATPGGRTFDFGFAAARLPDIRGETH
                     FTAAGPFYESAERKHDFRYLGVRPLFSRVSSPADARWSLDVLWPLAAARGWNDDFVWR
                     FLLAYGMRYGDEQSERYRTWLLPVWFQGRTAAGEDYFAVFPLGGKICEFLGRDRISFV
                     LFPLYARSVINEVRTTSILWPVGSRTKGDKISRFRVFPFYGYSRLLDEYEKRFILWPV
                     WTSARYYHEGGTGRGYVLFPLWGRLKLEDQESWMLLPPFFRWHRGEKRNVLHAPWPFF
                     QKTSGDVDKLYLWPLWGRREAAGIKTGFFLWPVFGRRVVDRQDTELKTLLAMPFLTWE
                     KETEKTGDQAVTARWFSLWPLCSYRREGDESRFRLLHLFPGRHIPPVERNWAPFWTLY
                     SRQRIGDERQSELLWGLCRSRKKNDAYAARSLFPLAEYEVSHRDGNRFEWSVLKGLIG
                     YKRVDETRSLRLFYLLKLGGKERQP"
     gene            8840..9586
                     /locus_tag="PKK36_06445"
     CDS             8840..9586
                     /locus_tag="PKK36_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR94235.1"
                     /translation="MLLTCRNTGRAVLMTLEAFLYLGDAFKKKNRHEVLYQLFITGIK
                     SLGVITVVALFTGMILALQTGLELRRFNQEVNIGTAVTVVMTREMGPFMAGLIIAASV
                     GSAIAAQMGTMVVSEEIAALELLSINPVRFLVVPRLWALMVMMPILTVYTNILGIVGG
                     GLVGATQLGVAMTAYFDNAVRYAANKDLYVGLFKSVLFGIIITTVACHQGFSTRGGAV
                     GVGIATRRTVVISFLTILVVGYMITRVFYS"
     gene            9583..10338
                     /locus_tag="PKK36_06450"
     CDS             9583..10338
                     /locus_tag="PKK36_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR94236.1"
                     /translation="MIRFKDVTRYLGGKPILNGMDLEVEQGETYVIVGASGTGKSVTL
                     KHMVRLLTPDTGEVWVGDDCVSSARGRDLAKIRMRFGYLFQSAALLQWCSVGSNVGLP
                     LMEHTGMSKDEIETVVREKLELVGLEEAYDKFPAELSGGMQKRVALARAIIMQPEIIL
                     YDEPTSGLDPVTSRTIDRLIDDMRRELGVTSVVVTHDLHSALVIGTRIAMIDQGRVIE
                     VAAPEEFVKSTRPEVQRFLEAQYITTRGEWERT"
     gene            10339..11331
                     /locus_tag="PKK36_06455"
     CDS             10339..11331
                     /locus_tag="PKK36_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNR94237.1"
                     /translation="MKSDRMKEMSMEVTVGAFMFMVLLTLGFFTIVLSQENIFKPGYE
                     ITAMFNNVLGLREGDNVFVHGVDVGKVKELKVTRNGVELTARLEVPLVFHEDYRIEIL
                     PSSVLGGRYLHIFAGTASSPLLPEGSTLHGVQPIDLLDEATEAVMLLRQAFDEGGMLD
                     DLQEAIANFRDLSKRLNEGEGTFGKLLTDAELYDNLRDITASFRDVADRLEKGEGSIG
                     KLLTDEAVYDDLKSFADDMKEIGDRVAGGRGTLGKLLSEDEQVYQDIADLVKSLKEVS
                     DSLNSTEGTIGKLVKDDEIYQEAKKLIREIRATIDDMRETSPITTFTSIFFGAF"
     gene            11333..11659
                     /locus_tag="PKK36_06460"
     CDS             11333..11659
                     /locus_tag="PKK36_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94238.1"
                     /translation="MKTPLFRIMAVVLSAVLALALPVPQCAAADDESDTDEIGKRVTA
                     GVLVLLLAVLVIIGFREDMDRRAEAALPVLPDTAAPKIFVDSDEVPADGGTMEIATRV
                     GLRMSF"
     gene            11808..12128
                     /locus_tag="PKK36_06465"
     CDS             11808..12128
                     /locus_tag="PKK36_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010874112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNR94239.1"
                     /translation="MSQTLEIIKLLLADGKVRISEHGYDELAADGLFVRDLVEGLVDA
                     ELLEDYPDFPKGPCILVLERDRNRQPVHVVWGIPKGRMDPAVLITAYRPEASRWSDDF
                     RRRK"
     gene            12128..12343
                     /locus_tag="PKK36_06470"
     CDS             12128..12343
                     /locus_tag="PKK36_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94240.1"
                     /translation="MSTRQHAKLVHEGDYVAEVDINLIEMDSDWSPCMSLDDAYRLDD
                     VRDALRRGDLKAASRLARVFTLTPVAV"
     gene            12504..12959
                     /locus_tag="PKK36_06475"
     CDS             12504..12959
                     /locus_tag="PKK36_06475"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR94241.1"
                     /translation="MRKTNRKSAFTLIELLVVIGIIGVLSAMIMGIAGLASRKSMRSK
                     CISTMGHIQNCLEEYRVKKNEYPTTLKSMYGVVPAEIYVPVGNFYGPLDGTTNEQTGA
                     IQEWKDPWGSDFVYQRTSGNRFSYKLWSKGPNSESKATSDDIVAQGSAQ"
BASE COUNT         2460 a         3773 c         4123 g         2663 t
ORIGIN      
        1 aactcaatcc cgccgaggcc ctgtgcgtcc gcaggaacct gggcgtgctg cgcgatcttg
       61 gattcggaat ctccgagttt ggcggccaga cgtacatggt ggatgccatg ccggcgtgcc
      121 tcggggcggt ttccgccgcg ccgattcttc gggacatgct cagccacatg gcggcaggcg
      181 gaacttcagg cgcggccaaa tggtcgcgcg agcagctggc gcaggccgcc tgctcggccg
      241 cggtgaacgc ccgcgtgaaa ctcacgctgg cggaaaccga aaagctggtg gtggatctgg
      301 cccgcgcccg gatgccctac acgtcgccac agggacggcc cacgctgatc ttcatgagtt
      361 tctcggagct gcgccggaaa ttcggccgct gatcacgacg acaggccgtt ggtttcgatt
      421 tccggaacca ggtcttcgcg gaagcaccgg caggcgcgtt catagcgctc cttctccgtt
      481 tcatccatgg cggccagaag cgccgtcttg agctcttcaa acggcttgca ggccatgcag
      541 atcacctgac ggatgtcggc gatgctttcc tcgacccgct ggttgtagac cgccgtgggc
      601 cgcgcggcgc ggtcctcctg ctgcagaaag cggaacagaa atccctcctc ctgctcgacc
      661 tggtgctcat gggtcagcag cgcccggagc gcctgcagat cgtgcccgat cagcatgagc
      721 aggtgattga aatccttctc cttgtctatg gccacagggg cccgtgcggc cagcgttgcg
      781 ttgcggctgg cggccgtgac caggcgcgtg atgttttcga tgtgctgcac cagccggtcc
      841 ctcccgatcc ggtcgagcgg gagcgatatc aggtcgtggg ccttttcgaa atacacggtg
      901 agctgcgcca gatggatcgg ccgattcagc tgaaaatgcg ttttgctcat aaccgttttc
      961 tgtcttcacc ctaccaaact tacgattcac ggggcaacag aatgttttcg gagggacgga
     1021 ctcttttgct gttgcactcg ccgccggatt cttcatgatg aagcacgtca tgaaaacttc
     1081 caatgattgg aaaatcgcgc gcaaaaagtt ccaatgattg gaaaaaatga gtccagaagt
     1141 tccaatgatt ggaaaaaacg gccgatgtcc tcgtatgcca gagtggtggt ggaaatagcc
     1201 ctgaacaggg aattcgacta catcattccc gaagcgctca gggatgccgt cgcggtgggt
     1261 tcgcaggtgg tggtgccgtt cggcaaatcg gaggtgcgag gcttcgtggt ggccctgccg
     1321 gagcgctcgg atcggaatga cctgcgcgag ataatccggc tggtggggaa gaagccgctg
     1381 atttccgggc gggtcatgga actggcccgc tgggtggcgg aatactacgc cgcgccggtc
     1441 gaaaccgccg tgcgcacggt gcttcccagc gccgtgcgcc agaaaaacgc ccgcttcaag
     1501 gagcagctct tcgtgatccc gatggatgcc gcctccgatg ccgaggccgt ccggcggctg
     1561 cgcgcgcgct ccccgcggca ggccgcggtt ttggacatcc tcctgtcagg cgaggccatg
     1621 ttcctcagcc accttgccaa gaccgcggga accacggccg gaacggttcg gggccttgaa
     1681 aaaaagggct tcgtgaggat cagccgcgag gcgatggctc gtgatccgct ggccggccag
     1741 acggttctcc gcacgcagca ccagaagctg atgccgcagc aggaggaggc ctttgcgctg
     1801 atccggcgcg ccatggacac gctggccccg ccggtcgtgc tgctctacgg cgtgaccgga
     1861 agcggcaaga cggaggttta cctgcaggcc attgatcatg ccctcaaaca ggggaagggc
     1921 gcgatcatcc tggtgccgga aatttccctg accccgcaga ccgtcgagcg cttccagagc
     1981 cgcttcgggg acgacattgc cgtgcttcac agccatctct ccggaggcga gcggcacgac
     2041 gagtggcacc gccttcactc cgggaaggcc cgcatcgcca tcggcgcgcg gtcagccctg
     2101 tttgccccga tcgaaaatct cggtctgatc gtggtggacg aggagcacga gcacagctac
     2161 aagcagggcg aggcgccgcg ctatcacgcc cgcgatgtcg cggtgatgcg cggtcacatg
     2221 gaaaggtgcg ccgtcgtgct gggcaccgcc accccgtcgc tggaatcctg gcacaacgcg
     2281 cagaccggaa agtacgcgct ggcgcggatg ccgcaccggg tggaccaccg caagatgccg
     2341 catgtgcgcg tgattgacat gcgcctcgag gcgtcaaaaa cgggacgggt gacgatgttc
     2401 tcatccgaac tggttgaggc cgttcgcgcg cgcctcgcgc gggccgagca ggtgatcctt
     2461 ttcctgaacc gccgcgggca ttccacctcg ctgatctgcc cgaaatgcgg ctatgtggcg
     2521 acctgcagcg catgcagcgt gcccctgacc taccacaagg tcaccgagga gctccgatgc
     2581 catatctgcg gcgccattga acccgttccg gaccgttgtc ccagcgaaga gtgccgtgat
     2641 ccccgcatcc gctatgccgg gatcggcacg cagaaagtgg agctggccgc ggccaagctg
     2701 tttccaaaag cgaacattca gcggatggat gccgatgtga ccacgcagaa ggatgcctac
     2761 agccgcattc tgggcgactt ccgggtgggg aagaccgaca tcctgatcgg tacgcagatg
     2821 atcgcgaagg gactgcattt cccgaatgtc acgctggtgg gtgtgatcgc ggcggatgtc
     2881 gcgctgcaca tgcccgactt ccgcgcgggc gagcgaacct tccagcttct cacgcaggtg
     2941 gcgggccgcg ccgggcgggg ggatgtttcc ggcgaagtca tcgtgcagac cttcaccccc
     3001 tttcatccct ccatccaggc gtcgagccgg attgattttg agggtttcgc cgaccaggaa
     3061 atggaattcc gccgggaact gttctatccg ccgtattctc atctcatctg catcaccgtg
     3121 cgcgggcgcg tcgaggagca ggtgaagttt tccattgatg ccttcgcgaa gacgctgttc
     3181 ccgatgcttc cggaaaaggt ggtcaggggc gaaccggcgc ccgcgccgct ggcgcgcacc
     3241 tgcgggcagt accgctggca gatcatgctg cgcgcggcgt ccgcgcgggc cgtcaccggc
     3301 ccgctgcgca cagcgctgga tcagttccgc tggccgaaag aggtccactg cactgtggac
     3361 gtggatgcca tttcgctgat gtgacggtca gcgctgcgac tcctgtatca tgggcggttt
     3421 gggtttgggc gaggccatgc gggccagttg ctgctgggtc gtttgctgaa agagaagcgt
     3481 tccgccggga tcaaagagcg tgacgattat tccatacatc tctttccctg tttttctctt
     3541 gtaaccgtca tatttcaggg agctgctgta ggtgccgatg tcaccggcat ccaccataac
     3601 ggattttccc cgggcaatgc cggaaatcgg gatgtgctgg actgcttcaa gcttcaagtc
     3661 gtcgttgacc gcactgcggc tgtaatactc gacggtcagc agagactgcg ggggaatggc
     3721 tttgtttctg gcgctgattt tgatgcgggg cgtgtggtag agaacgccgt cttcctgctg
     3781 gtatctgagg cagggcgatc catcccggac cactctgtac tggtctgaac ggattccgag
     3841 ccatgcgtcg aagctgtaga aagcggaacc cgcccggctt tcctgcgaag tgaaatacag
     3901 gcggcaggat ttaaagtctt tggaaggatc ggcggcgggc ggtttggatt catatatgac
     3961 gggtttggag ggataggaac cggtgctgtg aatcgtcatg ccgccctgct gggtgatcac
     4021 ctcggttgac gactggtttg ctgccgttct tgccggaagc acaacattca ctcccggctc
     4081 cgtccgggga tgcggatgag gccccgattc ctcctttttc ccctgcacca gataatccac
     4141 cagattcaga aacaaatcac gaccctgtgc tgacatgatg tcgggcgagc cggaatatcc
     4201 ccagaacaaa aagcgattgt cttccagaag caccggatag gcgccgggtc gggaggcgga
     4261 ctgcacaatg ggccgcagat aagatggcac agactgcagc ataacggccg ttcccgtttg
     4321 cattttgctg tacaaatcga acccgtttga tgaaattgga attggacgtg tcttcttcag
     4381 ggtgctgtgg agcggggtga tggggatgac gcggctttcc ggaaccatgg tcgcgtgctg
     4441 caccatggag agctccagct gacgaagaat cgttcttgcg gaatctccca cggctatgac
     4501 gggcacatcg ctgtttttca cgctcaaaag gaattcaatg aacgcatcac tgtccggcgg
     4561 catgtgtgcg cacgccagga caatcaggcg cgtacgttct gcgaccgctg cccgcagtcc
     4621 cgtgtaggga acggttgtga tgatgtagcc gccgtcttcc agcaggctct tataggaagc
     4681 cgtaatcagt ctgtcgggcc cgctcaccag cgttacagaa ttggattcca cttcgccttc
     4741 tgcctccagc cggttcgctc cggcaggcag accgtccgtt tcaggccgcc tgacttcagg
     4801 tgggcggaat tcgggcctcg cgtcctgtgc gcggcaggtc aggatcatgg ctgcggcagc
     4861 cagtattacg gaaagtgttt tcatctccag tgtatccctt tcttccctgt ttcactatac
     4921 ctgtattgca tcactcaatc catgcggttg ccgccgcgat gtcgtcgcga tagcgcggct
     4981 ttttcgccga atactgctcc gcaaggtgga tcatgtccgg caccagatgc tgccactggc
     5041 cgggatggag cagatgcccg cccgtgtcca ggaagttgac cagccatgcc tccagcgaaa
     5101 actgctcctc cgtgagccgg tagttgttca aatccagcag cccgcgtatc agaggcagaa
     5161 aaatcccgtc cgtggcggca aagacgcagc acggctccgg cagttcgacg gtgcgtttcg
     5221 aattttcaag cgcggcccgg acattctcgg tgtcgggctt gccccagtag ccgtgaaggg
     5281 tgcggctcag ttccagcagc tcgtttgaaa ggaaggtgta ctccttttcc actgccatga
     5341 ccatggccag gctgttctgg tcccgcgtca gcatcgttgc ggcgggggcc cggtccgggc
     5401 ggtgctcaag cagataggcg cggcagtcgc ccagctgcat cacgcggcac tcagagccgt
     5461 gcatcaggca gagggtgatg cagaacccga atatgtcgcg cctgtcgcgg ttgagcggga
     5521 tcagtgcgct gttgcttcgc agcagcagct ctgtgacggc cgcatccagt tcatccagag
     5581 ttcctgctct tccgttcgag atgatttccc cgagttccct gtcaaaagcg gagatgaatt
     5641 tcttcatcgg tccggtggcg gactgtccga tggtgtcaat ggcggcgatg accccgcggc
     5701 gctctgcgcc gttcagcgcc gtgtgatgca gggcgtagtc ggaaccgctg cgcggtttct
     5761 tgattccgct ggtctcaagc gcattcatgc ttcagcggct gaaacgaccc atggtctcgc
     5821 cctgcgccag aatatgaccc gccatggcct tctgcacgtc cttcatttct gcccgggggg
     5881 agaggtcgag cttcttgtcg agggcataaa cccggaaaaa atagcggtgc ggcctgcccg
     5941 gcggcgggca ggggccgcca tagccgggcc ggcggaagtc cgtgatgccc tgaatgctcc
     6001 cgttggggag ctcgggatcc ggttccacgt cctcgggaag ctcgcgcgct tcgggcggaa
     6061 gattgatcag cacccagtgc acccatcctc cggggcagtc ggggtcgtcg gagagaacgg
     6121 ctatgctctt cgtgcccgcg ggcaccgcgc cccacgacag cggcggcgag acattggctc
     6181 ccgcgcaggt gtattgggcc ggcatggcgt cgccgagtgc aaaggcggcg ctcacaacgg
     6241 tcagactgat gatgtccatg gctgttctcc tgtctgattc agcttccagc ctatcacagc
     6301 gggcgcagga ctcaacagcg gatttgcctg ccgtgccctt gcgggcctgc atattttgtt
     6361 gagtgcgcct cgtgttcgga gcatactccg gccatgaaaa tgatctgcgg aatcctggct
     6421 gcggccatgc tgctggcccg gcccgttccg gcggcggaag cggcggagcg caccgtggtg
     6481 ctggacgggg agttttatct ggacgaggac atcgtttttt ccaaaagcgg ccgcaccatg
     6541 gacattctgc atccggtgga aacctccggc ccgctgccgg ccattgtctg gattcacggc
     6601 ggcggctggg gggcggggga caagcggcgc ggcgcgcgca acatcctgcc ttttgccgag
     6661 cgcggatatg tctgtgtctc gatttcatac cggctgacgg atgaagccgt gttcccggcg
     6721 cagatccacg actgcaaggc ggcgatccgg tatcttcggg cgcatgccgg caaatacggc
     6781 attgacccgg accggatcgg cgtctggggc gcttccgcag gcgggcatct ggcggccctg
     6841 ctgggcacct ccggcggcat caccgggctg gagggcgatg aaggctggcc cgacttttcc
     6901 agccgtgtgc aggccgtctg cgactggtac ggtcccgtgg acttctacag cgcggcccat
     6961 caggcgccgg aagatcgtcg gccggaaaag ctgctgggcg gggaagtcct ggaaaaccgc
     7021 gaactggcgg cgctcgccag cccggtgtcg catgcaacgc ccgatgatcc tccctttctg
     7081 atcatgcacg gaagccgcga tgatgttgtg ccgatgaagc agagccgcct gctgaaatgg
     7141 gcgctgagcc ggcagggagt tccggtgtat ttctctgttg tgcggggcgg cgaacacggc
     7201 ggtgaacgat ttgagcagga gaaggttcgt gcgaaggtca tgaagttctt tgatgcccat
     7261 ctcaagggcc ccggaccggc acaggctctg ccttagcgga gtgtccgggt gttgcgctcg
     7321 gaagaggaat gcgctatcgt cgggcgcaaa tgaaatggta cttccagtcg ggcattctgc
     7381 tgtgcgccct gcttctcgcg ggatgcgcca cccccggagg gagaactttc gacttcggtt
     7441 ttgctgccgc ccgcctgccg gacattcgcg gcgaaacgca tttcaccgcg gcgggacctt
     7501 tctatgaatc cgcagaaagg aaacacgact tccgctatct gggagtccgc ccgctgtttt
     7561 cgcgcgtgag cagtccggcc gatgcccgct ggtcgctcga tgtgctctgg ccgctggccg
     7621 ccgcgcgcgg ctggaacgat gactttgtct ggcggtttct gctggcctat ggaatgcgct
     7681 acggggatga gcagtcggag cgctaccgca catggctcct gccggtctgg tttcagggac
     7741 gaaccgccgc cggggaggac tacttcgccg tgttcccgct gggcggaaaa atctgcgagt
     7801 ttctcggtcg ggaccgcatt tcctttgtgc ttttccctct gtatgcccgc agcgtgatca
     7861 atgaagtgcg gaccacttcg atcctgtggc cggtgggctc gcgcacgaag ggcgacaaga
     7921 tcagccgctt ccgcgttttt cccttttacg gatacagccg ccttctggat gaatatgaaa
     7981 agcggttcat cctgtggccg gtctggacct cggcgcggta ttaccatgaa ggcggcacgg
     8041 gcaggggcta tgtgctgttc cccctgtggg gccgcctgaa actggaggac caggagtcgt
     8101 ggatgctgct ccctccgttt ttccgctggc accgcgggga aaagcggaac gtgctgcacg
     8161 ccccgtggcc gtttttccag aagacctccg gtgatgtgga caagctttat ctctggccgc
     8221 tgtggggccg ccgcgaagcg gcggggatca aaaccggttt cttcctctgg cccgtattcg
     8281 gccgccgggt ggtggaccgg caggacaccg aactgaaaac cctgctggca atgcccttcc
     8341 tgacatggga aaaagagacg gagaaaaccg gcgatcaggc cgtgacggcg cgctggttca
     8401 gcctgtggcc cctctgttcg taccgccggg agggcgacga gagccgtttc cgcctgctgc
     8461 atctttttcc gggccggcat atcccgccgg tggagcgcaa ctgggcgccg ttctggaccc
     8521 tctattcgcg gcagcgcatc ggggatgagc ggcagtcgga gctgctctgg ggactgtgcc
     8581 gcagccggaa gaaaaacgat gcctatgccg cccgctcact tttccctctt gccgaatacg
     8641 aggtgtcgca cagggacggg aaccgttttg agtggtcggt gctgaagggc ctgatcggct
     8701 acaaacgtgt tgatgagaca aggtctttaa ggctattcta tcttctcaaa ttgggcggaa
     8761 aagagaggca gccataatca caagagagcc agaatatttc gaggcgccgc tgaactggtt
     8821 tggttcgttc ggccggcggg tgctgctcac ctgccgcaac accggccgcg ccgtgctgat
     8881 gacgctggag gcctttctct atctcggcga cgccttcaaa aagaagaacc ggcatgaagt
     8941 gctgtaccag ctgttcatca ccggcatcaa gagcctgggg gtgatcacgg tggtggcgct
     9001 cttcacgggc atgatcctcg ccctgcagac gggactggaa ctgcgccgct tcaatcagga
     9061 ggtcaacatt ggaacggccg tcaccgtggt catgacgcgc gagatggggc cgttcatggc
     9121 gggcctgatc atcgccgcga gcgtgggatc ggccatcgcg gcgcagatgg gcaccatggt
     9181 ggtctccgag gaaatcgccg cgctggagct gctgtccatc aatccggtcc gcttcctggt
     9241 ggttccgcgg ctctgggcgc tgatggtcat gatgccgatc ctgacggtct atacgaacat
     9301 cctcgggatc gtgggaggag gactcgtggg ggccacccag ctcggggtgg ccatgaccgc
     9361 ctatttcgac aatgcggtcc gctatgccgc gaacaaggat ctttacgtgg ggcttttcaa
     9421 gtccgtgctg ttcggaatca tcatcaccac ggtggcctgc catcaggggt tttccacccg
     9481 gggcggggcg gtcggggtcg gcattgccac ccggcgcacg gtggtgattt cgttcctgac
     9541 gatcctggtg gtcggctaca tgattacacg ggtgttctac tcatgatccg ctttaaagac
     9601 gttacgcgct atctcggggg aaagccgatt ctgaacggca tggacctcga ggtcgagcag
     9661 ggcgagacct atgtcatcgt tggggcttcc ggcaccggca agagcgtgac gctcaagcac
     9721 atggtgcggc tgctgacccc ggacaccggc gaggtgtggg tcggcgacga ctgtgtcagc
     9781 agcgcgcggg gccgcgatct ggcgaaaatc cgcatgcgct tcggctatct gttccagagc
     9841 gccgcgcttc tgcagtggtg cagcgtgggc agcaatgtcg gcctgccgct gatggagcat
     9901 accggaatga gcaaggacga gatcgaaacg gtcgtgcgcg agaagctgga gctggtgggg
     9961 ctggaggagg cctacgacaa gtttcccgcc gagctttccg gcggcatgca gaagcgcgtg
    10021 gcgctggccc gcgccatcat catgcagccg gaaatcatcc tctacgacga gcccacctcc
    10081 ggcctcgatc cggtcacatc acggaccatt gaccggctca ttgacgacat gcgcagggag
    10141 ctgggcgtca ccagcgtggt ggtcacgcac gacctgcaca gcgcgctggt catcgggacg
    10201 cgcatcgcga tgattgatca ggggcgggtc atcgaggtgg ccgcgccgga agagtttgtg
    10261 aaatcaaccc gcccggaggt gcagcgtttt ctggaggcgc agtacatcac cacccgcggc
    10321 gagtgggaaa ggacatagat gaaaagcgac cggatgaagg aaatgtcaat ggaggtcacc
    10381 gttggggcct tcatgttcat ggtgttgctg acgctggggt tcttcaccat cgtcctcagc
    10441 caggagaaca tcttcaagcc cggctatgaa atcacagcca tgttcaacaa cgtgctgggc
    10501 ctgcgcgaag gcgataacgt ctttgtgcac ggcgtggacg tgggcaaggt caaggaactg
    10561 aaggtcacgc gcaatggggt ggagcttacc gcgcgcctgg aagtcccgct ggtgttccat
    10621 gaggactacc gcattgaaat cctcccgtcc tccgtcctgg gcggccgcta cctgcatatc
    10681 tttgccggca cggcctcttc gccgcttctc ccggagggat caacactcca cggtgtgcag
    10741 cccattgacc tgctggatga agccaccgag gcggtcatgc ttctgcggca ggcgtttgac
    10801 gagggcggca tgctggatga tctgcaggag gccatcgcca atttccgcga tctgtcaaaa
    10861 cgactgaacg agggcgaggg caccttcggg aaactcctta ccgatgcaga actctacgac
    10921 aaccttcgcg atatcaccgc ctcgttccgg gatgtcgccg accggcttga aaagggcgaa
    10981 ggctccatcg gcaaactgct gaccgacgag gcggtctacg acgacctcaa atccttcgcc
    11041 gatgacatga aggagatcgg tgaccgcgtg gcggggggca gggggacgct cgggaagctt
    11101 ctttcggagg acgagcaggt ctatcaggac atcgcggacc ttgtgaaatc attgaaagag
    11161 gtctccgact cgctcaacag caccgagggc accattggca agctggtcaa ggatgatgaa
    11221 atctatcagg aggccaaaaa gctgatccgt gaaatccgcg ccaccataga cgacatgcgc
    11281 gaaacctcgc ccatcaccac ctttacgagc atcttctttg gagcattttg acatgaaaac
    11341 tccactgttt cggatcatgg ccgttgtcct gtccgccgtc ctggcgctgg cgcttcccgt
    11401 gccgcagtgc gcggccgccg atgatgagtc cgacaccgat gaaatcggca agcgggtcac
    11461 cgcgggcgtg ctggtgctgc tgctggcggt gctggtgatc atcggctttc gcgaggacat
    11521 ggaccgccgc gcggaggccg cgctgccggt tctgccggat accgccgctc cgaagatatt
    11581 cgtggactcg gatgaggttc cggccgacgg cgggaccatg gaaatcgcca cgcgggtcgg
    11641 cctccggatg agcttctgac cggacggcgg acattaaaaa acttccaatc attggaactt
    11701 ttcggtaaaa aacttccaat gattggaact tttccgggaa aaacttccaa tcattgggaa
    11761 aatcaggcgt gaccacggct gtgccgcgct gacaaagctt gcggattatg agtcaaactc
    11821 tggaaatcat aaaattgctt ttggcagatg ggaaagttcg aatatctgag catggatacg
    11881 atgaactggc agcggatggc ttatttgtca gagatctggt tgagggcctg gttgatgcag
    11941 aattattgga agattatcct gatttcccga aaggtccgtg tatattggtt ttagagcggg
    12001 atcgaaaccg gcagcccgtc catgtggtat ggggcatccc caaggggcgg atggaccccg
    12061 cggttttgat aactgcttat cgtcctgagg ccagccgctg gtcagacgat tttagaagga
    12121 gaaagtaatg agtacaaggc agcatgctaa actggtgcat gaaggcgact acgtggcgga
    12181 ggtggacatc aatctgattg agatggattc agattggtct ccctgcatgt cactggatga
    12241 tgcataccgg cttgatgatg tgcgggacgc gctgcgccgt ggcgacctca aggctgccag
    12301 ccgtttggcc cgtgttttca cgctcacccc cgttgctgtg tgaagatata ttggagcgca
    12361 tacaatgcag acaggaagga tgcatgccga atcttcccca tccctgcatc aatccaatat
    12421 tccaccattc cattcttcca taccatcatt cccatccgtg aaatcagtgt aatccgtggt
    12481 aaaatatcgt taggagacga cgtatgcgaa aaacaaacag aaaatccgct ttcaccctca
    12541 tcgaacttct tgtggtcatc ggcatcattg gcgtgctgtc cgccatgatc atgggcatcg
    12601 ccggtctggc ctcgcgcaaa tccatgcgct ccaaatgtat ctccaccatg gggcatattc
    12661 agaactgtct tgaggaatac cgtgtgaaaa aaaacgaata tccgacaacc ctgaaatcaa
    12721 tgtatggcgt ggttccggcg gaaatctacg ttccggtcgg caatttttac gggcctctgg
    12781 acggaaccac caatgagcaa accggcgcaa ttcaggagtg gaaggatccc tgggggtcgg
    12841 atttcgttta tcagcggacc agcggcaacc gcttcagcta caaactgtgg tcgaagggcc
    12901 cgaattccga atccaaggcc acttcggacg atatcgtggc gcagggcagc gcgcaataag
    12961 aggactccgt ctggagggcc gacctctgtg tcggccgtga acagggattg agaatgaat
//
LOCUS       DAOQWR010000063        12964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5319_length_12964_cov_4.883725, whole genome shotgun sequence.
ACCESSION   DAOQWR010000063 DAOQWR010000000
VERSION     DAOQWR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12964
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5319_length_12964_cov_4.883725"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..910
                     /locus_tag="PKK36_06480"
     CDS             143..910
                     /locus_tag="PKK36_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94242.1"
                     /translation="MKKILHILGLTMIFAPGALQAQSAREALKAGSREFADGAYDKAA
                     AHFEEAASLAPKQKLDPAIAHYNRGNALYKMQDFAGARACYENALRTLDTDLQSTIYF
                     NLGNLTFHSATNMTSFDQHVAVLDQSMDYYMRSVFLHPDSRNAKVNYEMLYRMKNEML
                     VARDTLYKVLQAAKKQVRDGRLEVAMEDCKQFMSDAEVQRALSLIPEAQKDYQELLQK
                     LQELIDVVEKAKGMTAELKAQQEALDSLQQEAEHEAD"
     gene            1089..1370
                     /locus_tag="PKK36_06485"
     CDS             1089..1370
                     /locus_tag="PKK36_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94243.1"
                     /translation="MLKELLEKSSQQQQEQEQPQNQPEQNQDQQNQQPQEEQSGQDEQ
                     QEQPQPKPSDEMSKEEAEMMLDAMKKNEKSQREQIQMLLGKPLPVDKDW"
     gene            1442..3190
                     /locus_tag="PKK36_06490"
     CDS             1442..3190
                     /locus_tag="PKK36_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNR94244.1"
                     /translation="MAFLCLAATGSAQPGVSFDIQPRVLRVGQPAQCTITVRGADNPS
                     APALPPMDGFQISGPGTSRQSSFNLVNGQMQSDSSVTFTYSLLPLKAGQFTIGPFEYQ
                     MGNQKAELPAIQIQVVQPEGGSDSEALYAEITSARENPFVQEVFDLYITIHVHESINL
                     HPEISLLGFDTAGLDMKGFQELGQTREVVDNTIFNVRRFRAKARALRSGDFSLNPVLR
                     AHVIVSRDQRRSDPFRNPFLSGFFNQQETRAVNLAVKPLTLHVRPLPEDGKPASFSGA
                     VGRFDFQVAAKPLELAVGDPITLTLTISGEGNLDGITAPAPSAGEYFRTYDIKQVARQ
                     TDAGGTAGQLVFEQVLLPQNINAKELPSIEFGFFNPQQEKYVTLSRGPFPLRLSPSAN
                     GGSRMVDGSSRAAAAGAKILGSDIVYLKPAPRSFVQDKPRSRGFWLLQTVPALCAAGV
                     FALSRRRRELNRDVARARRIRAPRSARRGLRMMNDAAASADAARFTEGLWETLTRYFG
                     NLLNLAPGEVTADVVAHRLGHCGLPQETVEDIAQLFRACDSMRYAPGQGVTSSDMAEM
                     KYKLERIIKSCERIKS"
     gene            3187..3957
                     /locus_tag="PKK36_06495"
     CDS             3187..3957
                     /locus_tag="PKK36_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94245.1"
                     /translation="MKTAKPILFLMLMLSLTAPGRLFAGFTELFQQAAGAYDEGRFAD
                     AARLYEDLLNAGAGSPEVFYNLGNARFKNGEDGLAVLNYRRAQYFSPRDPDIRANLDY
                     AMSRNSAVRRSQTTAAQLAENLSLREWENAMLTFYWLAAAAAILWMLDAARRPVYSKL
                     LMLLAVLLVIGTAGVRHWKDLQRMPEAVMVETGRSAFFAPIESSTAHFSLPVGSLVRI
                     HEFEGRWLKVEADGKEGWIPDQSCRAVYPWISPADQQE"
     gene            3965..4177
                     /locus_tag="PKK36_06500"
     CDS             3965..4177
                     /locus_tag="PKK36_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94246.1"
                     /translation="MRDNIHRHQTSWLWAAALVILLIGGTITWLLATQGYSLRAQKAM
                     ILFLASTIIGVGICVISATSHWWLHK"
     gene            complement(4196..6241)
                     /locus_tag="PKK36_06505"
     CDS             complement(4196..6241)
                     /locus_tag="PKK36_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNR94247.1"
                     /translation="MISPARGRSGEKACGEETFPSRADAFFRPGGLLEEACHAEEFPY
                     EERPQQRTMACAVAGVIHKSSHLAVEAGTGVGKSFAYLVPLIYASLERRERMVVATYT
                     ISLQEQLIYKDIPFLQKVLGDGFKAVLVKGRSNYLCLRRLGRTFRMGKDLFAGAGRDE
                     VAEIRKWAETTEEGSLQSMAVQPSSEIWSQVCAEHGNCLGRKCPHFKPCFFMRARAQM
                     QEANLLVLNHHLLFSDIQLRRHRAAFLPKYTVIVLDEAHQMEDVASEHFGIRLSHYAF
                     EHWMRRLYVSDTNKGLLAALRRGDAAHEVTELGRAVDDLYLKFKRWAAFRGDETQRVL
                     TAPPALETDVPARLDRLAKMLEEIIENTEDDDVLAELEAIRRHGMGMAEELRSFLSQS
                     LPDQVYWVEKEGRRRSLMVLHAAPIEVAPVLSEALFKSSPCVVMTSATLAVGGSLEYF
                     KTRVGAENCESLETGSPFNYQQQMRVLIPRKMPDPKDMNEYLPAVTRAVEHFVIESRG
                     RAFVLFTNAGLMRKVAAATEPVFTGAGLQLFVQGAGLARHVMLDRFRESPAGVLFGVS
                     SFWMGVDVRGGALSNVIITRLPFAVPDQPLIKARLDRIKEKGGDPFKEYSLPEAILRF
                     RQGVGRLIRTNTDEGRIIVLDRRIADKWYGRLFFSAIPECPVEEVDIPGDEDEAFPA"
     gene            6354..6428
                     /locus_tag="PKK36_06510"
     tRNA            6354..6428
                     /locus_tag="PKK36_06510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6386..6388,aa:Gly,seq:gcc)
     gene            complement(6556..6640)
                     /locus_tag="PKK36_06515"
     tRNA            complement(6556..6640)
                     /locus_tag="PKK36_06515"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6604..6606),aa:Leu,seq:tag)
     gene            complement(6655..6730)
                     /locus_tag="PKK36_06520"
     tRNA            complement(6655..6730)
                     /locus_tag="PKK36_06520"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6695..6697),aa:Ala,seq:ggc)
     gene            complement(6798..7655)
                     /locus_tag="PKK36_06525"
     CDS             complement(6798..7655)
                     /locus_tag="PKK36_06525"
                     /inference="COORDINATES: protein motif:HMM:NF039394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HNR94248.1"
                     /translation="MKTSVLLLLIPLATAWGMESIVPEDPFAWGLFTAADAASFDNED
                     GTEGEAAWSEASMAAWLPLAESPNLQWFSGAGLTVSRFEFDDSPLDDLDVYSAVLYLD
                     ALYLPNDRWEIIVELAPELASDMKDASLSDAELSASALATWKWREGWQLTLGAAYDRE
                     FGEDLLYPMGGFIWDASPMWRVAMVLPAPRISCALSRDIVLFTDLQPAGNVWKLEEET
                     DFEDHELKVEAWRWALGAEMRVWRRVWMHAAVGMETGREYEIRSHQRRLLDSEVDDVT
                     FARLGFVVR"
     gene            complement(7659..9374)
                     /gene="recJ"
                     /locus_tag="PKK36_06530"
     CDS             complement(7659..9374)
                     /gene="recJ"
                     /locus_tag="PKK36_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNR94249.1"
                     /translation="MKVWKTASFQESAAKAIRDAHGLPHPVAAVLAARPWASAEETAQ
                     FLSPSLSSLGDPFLLPAMEPAVDRIWKALREKQPVVVYGDYDVDGVTSTAMICRVLAR
                     LGAEVHPVLPHRVDDGYGMSMEPVTRCMDEYRPGLIVTVDCGTGSREAVEYAAAQGVD
                     VVVTDHHEASGEVAPALALVNPKLGDDPRSKLLAGVGVAYKLCHALVKKGREAGESSA
                     LDIDLREYLDLVALGTVCDIVPLLYDNRVLVRAGLERLARTGNAGLKALLEVAGTKPP
                     YTAYALGFQLGPRLNAAGRLGTAMHALNLLMTGDAAEAARLAGDLDRTNRERRDIETE
                     IFSLASAQLDESFDGKKDYALVAAGEGWNQGVVGIVASRLVRKYNRPAVVIAVGEDGV
                     GKGSCRSIPGFNMVENLQACAALLTRWGGHAMAAGLELPASRIGEFREQFNRIASVAL
                     RHADLRPSITIDAWLEPGEFGAALYEGQKKLEPFGMDNPAPLWGLRGLAPAGTPRTVG
                     SGHLKFSLDVNEAAVPAIFFGWGDRPLPDGPLDVAFQMKLNSFRGTDNLEMDVKAVRP
                     SKQGE"
     gene            complement(9445..11940)
                     /gene="secD"
                     /locus_tag="PKK36_06535"
     CDS             complement(9445..11940)
                     /gene="secD"
                     /locus_tag="PKK36_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNR94250.1"
                     /translation="MEKNAVWKWLLLVVLLAGSFAVVYPPSQKVKLGLDLQGGTSFTV
                     EIDKEEIRKRILDDEPDADPDTLPARVARAASEARENALEVIRNRVDGLGIAEPVIYS
                     SSAEGSERIVVQLPGIDKEKREAARESIESVAFLEFRLVHEKSSEWVEELFSDFKAPR
                     GFKISDRGDYYIRDTETVKDAEMDQAFLETLRRFEPKPGCAFMLEKDTDASGRTIYKP
                     YYISIRRQMTGDTIKNAGVDYHQVTGAPRVTLEFDRQGARRFAKVTRDYAPRGEKNRD
                     SDLGRQLAIILDGTLYSAPVIQTEIPNGRAEITGNFKVPEAMRLANVLRAGALPAPVK
                     IIQTSTVDPSLGRDSIRSGSRAAVVAVAAVLVFMLVYYCAAGLVANAGLLLDLLLLPL
                     GLIVASGFLGLFSGQRFTGAAVSLPTLTMPGIAALVLTLGMAVDANVLIFERIREELR
                     VAKRLPSAITAGFERAFTTIFDSNITTLLTAVILFAFGSGPVRGFAVVLSAGIVVSML
                     VALVYTRLMLSSMVEGFKMKSLRMFSFFRDTKLDFIGKKYATWGLSLAIILGTWFVFV
                     QNGQKNFAVDFAGGTSLLYGFTEKAPVEQIRGDLAALNIDAGLQYKADLIADDDAGIN
                     EMLDVRVGENDGDAVKNLFEEKYAGYTLLQEEQVGGQIGAEMRRKGIMALVWSLVGIV
                     VYISIRFEFAYAVGAIAALAHDVLITVGIYCLLGRQISLPIVAALLTIVGYSVNDTIV
                     VFDRIREDLKLVRGRSYKDIVNLSINQTLSRTVLTSLTTLLSVVILLLFGGGAIFDFA
                     LTLLIGIIVGTYSSVFLAAPIMLLWHRDKPKAA"
     gene            complement(12170..>12964)
                     /locus_tag="PKK36_06540"
     CDS             complement(12170..>12964)
                     /locus_tag="PKK36_06540"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR94251.1"
                     /translation="HEADILDMDKLSAAMAGHDLVWHLAANTDIIGGVEQPRRDLRDC
                     VMGTFNVLEAMRATGIQPVIFSSTGAVYGDLCFDVATSEASGPLLPVSTYAAGKIGSE
                     AFISSFCHLYGLRAWMFRFGNVIGARMTHGVIFDFIHKLRANPQSLLIRGAGNQEKNY
                     FLVEECIDGMAYAFRNIPMTEDKPCDLFNLGTDSITRVTDIAEIVKTEMGLEDAEIKI
                     EGTKQAWPGDQPRVHITVDKMRALGWTARRTSDQAVAAAVRRMLGK"
BASE COUNT         2775 a         4057 c         3686 g         2446 t
ORIGIN      
        1 agaaccacat cgcgccgctc cagcgcgagg accgggattc caggatgctg aaagcgcatg
       61 aagaccggtt tgcgtggttc ctggcggcgg cgttcctgtt gctgttcatt gaagcggcta
      121 ttcccgagcg gatgaaagag tcatgaagaa gatccttcac atcttggggc tgacaatgat
      181 ctttgcgccc ggcgcgctgc aggcccagtc cgcgcgcgag gcgctcaagg caggttcaag
      241 ggaatttgcg gacggtgcct atgacaaggc cgccgcgcat tttgaagaag ccgcgtctct
      301 ggcgccgaaa cagaaacttg atcccgccat cgcccactac aaccggggca acgcgcttta
      361 caagatgcag gacttcgcag gggcaagagc ttgttatgaa aatgccctgc gcacactcga
      421 cactgattta cagagcacaa tctatttcaa tctcggcaat ctgactttcc attctgcaac
      481 gaacatgacc tcgtttgatc agcatgtggc cgttttggat cagagcatgg attattatat
      541 gcgttcagtg tttcttcatc ctgattcccg caacgctaaa gttaactacg aaatgctgta
      601 ccgcatgaag aatgaaatgc tggttgcgag agacaccttg tataaagttc tccaggctgc
      661 gaaaaaacag gtcagagatg ggcggctcga agtggctatg gaggattgta aacaattcat
      721 gagcgatgcc gaagttcagc gtgcattgtc cttgataccg gaagctcaga aagactatca
      781 ggaactgctt caaaaactgc aggaactcat tgacgttgtc gagaaagcta agggcatgac
      841 tgcagaattg aaggctcagc aggaggcgct ggacagttta cagcaggagg ccgagcatga
      901 ggccgattag gttaatccat ctgttgtcag cgatactaat cccggtaatg acctgcccgg
      961 caatcgcaga gacaccttac ggctatttca acgaatcggc ttatctctat acagacaaga
     1021 agcaggaaaa agcccttgag aaggtcaata ctggcctgta gtcctattct gataatccaa
     1081 aacttaaaat gctgaaggaa ctcctggaaa agtcctcgca acagcagcag gagcaggaac
     1141 agccgcagaa tcagccggaa cagaaccagg atcagcagaa tcagcaaccc caggaagaac
     1201 agagcggaca ggacgagcaa caggagcagc cgcagccgaa gccctcagat gagatgtcta
     1261 aggaagaggc cgaaatgatg ctggatgcga tgaagaagaa tgaaaagagc cagcgcgagc
     1321 agattcagat gcttctgggc aaacctctgc ccgttgacaa ggactggtag aatatttgag
     1381 aatgaccggg atgacattaa gcagcagata cgcaagaagg atgctccggg ctttcagcct
     1441 catggccttt ctgtgtcttg ccgcaaccgg ctccgcgcag ccgggcgtca gctttgatat
     1501 ccagccccgc gtgctgcgcg tgggccagcc cgcccagtgc acgatcaccg tgcgcggagc
     1561 cgacaatccc tccgccccag ccctgcctcc gatggatggt ttccagattt caggtcccgg
     1621 cacctctcgc caaagctctt tcaacctggt caacgggcag atgcaatccg acagttcggt
     1681 cacgttcacc tattctcttc tgcccctcaa ggccggccag ttcaccatcg gcccgtttga
     1741 atatcaaatg ggaaatcaga aggccgaact ccccgccatt cagattcagg tggttcagcc
     1801 cgaaggcggc agcgacagcg aggcgctcta tgccgagatc acttctgcaa gggagaaccc
     1861 gtttgtgcag gaggtgttcg atctttacat cacgatacat gttcacgaaa gcatcaatct
     1921 gcatcccgaa atcagcctgc tgggattcga cacggccggg ctcgacatga agggttttca
     1981 ggagctcggc cagacgcgcg aggtggtgga caatacgatc ttcaatgtcc ggcgcttccg
     2041 cgcaaaggca agggctcttc gttccggaga tttttctctc aatcccgttt tgcgcgcaca
     2101 cgtgattgtc tcgcgcgacc agcggcgaag cgatcccttc agaaacccat ttttgagcgg
     2161 ttttttcaat caacaggaaa cgcgggcggt taatctggcg gtcaaacccc tcacgcttca
     2221 tgtccggcct cttcccgagg acggtaaacc ggcttctttt tccggggcgg tcggccgctt
     2281 tgattttcag gtcgccgcaa aacccctcga actggctgtc ggagacccca tcaccctgac
     2341 ccttacgatc agcggcgaag gcaatctgga cgggattacg gcgccggcgc cgtctgcggg
     2401 tgaatatttc cgcacttacg acatcaagca ggtcgcccgg cagacggatg caggcggaac
     2461 agccggacag ctggttttcg agcaggttct gctcccccag aacatcaatg cgaaggaact
     2521 gccctccatc gagttcggat tcttcaatcc gcagcaggaa aagtatgtga ccctgagccg
     2581 cgggccgttc cccctgcggc tctccccctc cgccaacggc ggcagccgca tggttgacgg
     2641 ctcgtcccgg gcggccgccg cgggcgcgaa gattctgggt tcggatatcg tttatctcaa
     2701 gcctgcgccc cggtcctttg ttcaggacaa accccggtcg cgcgggttct ggctgctgca
     2761 gacggttccc gcgctgtgcg ccgcaggcgt cttcgccctc tcgcggcggc ggagagaact
     2821 gaaccgcgat gtggccaggg cccggcgcat ccgcgcgccc agaagcgcac gcagggggct
     2881 gaggatgatg aacgatgccg cagcctccgc ggatgccgcc cgcttcacgg aaggtctctg
     2941 ggaaactctc acccgttatt tcgggaacct gctcaatctg gcccccggcg aggtgaccgc
     3001 ggacgtcgtg gcgcacaggc tcggccactg cggcctgccg caggaaacgg tcgaagacat
     3061 tgcacagctt ttcagagcct gcgacagcat gcggtatgcg ccgggccagg gagtcacctc
     3121 atcagacatg gcggaaatga aatacaaact ggaacgaatc atcaaatcct gcgagcgcat
     3181 caaatcatga aaacggcaaa acccatcctg tttctgatgc tgatgctttc gctgacggca
     3241 ccgggccggc tttttgccgg tttcacagaa ctgtttcagc aggcggcggg cgcctatgat
     3301 gaaggccggt ttgcagacgc cgcccgtctc tatgaagacc tgctgaacgc gggcgccggc
     3361 tccccggagg tgttctacaa tctcgggaat gcgcggttca aaaacggcga agacgggctg
     3421 gcggttttga actaccggcg ggcccagtat ttctcgccgc gagacccgga tatccgcgcg
     3481 aacctggact acgccatgag cagaaacagc gctgtccgca gaagccaaac cacggcggca
     3541 cagctggccg aaaacctgag tctccgcgag tgggaaaatg ccatgctcac cttctactgg
     3601 cttgcggcag ccgccgccat tctctggatg ctggacgccg cccggcggcc ggtctattcg
     3661 aaactgctga tgctgctagc cgtgcttctg gtcatcggca cggcaggggt ccgccactgg
     3721 aaagatctgc agcgcatgcc cgaagcggtc atggtcgaaa ccgggcggtc ggcgtttttc
     3781 gcccccattg aaagctcaac cgcacacttc agcctgccgg tcggatcgct tgtccgtatt
     3841 catgaattcg aaggccgctg gctgaaggtg gaagcagacg gcaaggaagg atggattccc
     3901 gatcaaagct gccgcgcagt ttatccttgg atttcaccgg cggatcagca agaatagcac
     3961 ggccatgagg gataatatac acagacacca gaccagctgg ctctgggctg cggcgctggt
     4021 gattcttctg atcggcggca ccatcacctg gctgctggcg acgcagggct acagccttcg
     4081 ggcccagaaa gccatgattc ttttcctggc ctcaaccatt attggtgttg gaatttgcgt
     4141 gatcagcgcg acctcccact ggtggcttca taaatagggc gttcctgaaa tttcgtcacg
     4201 cggggaacgc ctcatcctcg tcgccgggaa tatccacttc ctccaccgga cattccggta
     4261 tcgccgaaaa aaacaggcgg ccgtaccact tgtccgcaat acggcggtcc agcacaatga
     4321 tgcgcccctc gtcggtgttg gtccggatca gccgccccac cccctgacgg aatctcagaa
     4381 tggcctccgg gagcgaatat tctttgaagg ggtcgccgcc cttttccttg atccggtcaa
     4441 gcctcgcctt gatcagcggc tggtctggaa cagcaaaggg aagtcttgta atgatgacat
     4501 tgctcagcgc gccgccacgg acatccaccc ccatccagaa actgctgacg ccgaacagca
     4561 cccccgccgg cgattcgcgg aaacgatcca gcatgacatg acgcgcaagg ccggcgccct
     4621 gcacgaacag ctggagaccc gccccggtga acaccggctc ggttgcggcc gccactttgc
     4681 gcatcaggcc cgcgttcgtg aagaggacaa aagcgcggcc acgcgattcg atcacaaaat
     4741 gctcgacggc ccgcgtgacc gccggcaaat actcgttcat atccttcgga tcaggcattt
     4801 tcctcggaat cagcacacgc atctgctgct gatagttgaa gggcgagccg gtctccagcg
     4861 actcgcagtt ctccgcgccc acacgggttt tgaagtactc cagtgatccg cccaccgcga
     4921 gcgtggcgct ggtcatgacc acgcacggac tggacttgaa aagcgcttct gaaagcacgg
     4981 gcgcgacctc gatcggcgcg gcatgcagca ccatcagact gcgccgccgg ccctccttct
     5041 cgacccagta cacctggtcc gggagcgact gcgaaaggaa gctccgcagc tcttccgcca
     5101 tgcccatgcc gtgccgccga atggcctcca gttcggccag cacatcgtca tcctccgtat
     5161 tctcgatgat ctcctccagc atctttgcca gacggtccaa ccgtgcgggc acgtctgttt
     5221 ccagcgcggg cggggccgtc aggacgcgct gggtctcatc tcccctgaag gcggcccagc
     5281 gcttgaattt cagatagaga tcgtccacgg cgcgcccgag ttccgtgacc tcatgcgcgg
     5341 catcgcccct gcgcagcgcg gccagcagcc ccttgttcgt gtcggaaaca tagagcctgc
     5401 gcatccagtg ctcgaaggca taatgagaaa gccggatgcc gaaatgctcg ctggccacat
     5461 cctccatctg atgcgcctca tcaagaacaa tgactgtgta cttcggaaga aacgcggccc
     5521 ggtgcctgcg aagctggatg tcggaaaaca gcagatgatg attcagcacc agcaggttcg
     5581 cctcctgcat ctgcgcccgg gcgcgcatga aaaagcaggg cttgaagtgc gggcatttcc
     5641 ggccgagaca attgccgtgc tccgcgcaaa cctgcgacca gatttccgat gacggctgca
     5701 cggccatgct ctgcagcgag ccttcttcgg tcgtctcggc ccatttccgg atctcggcca
     5761 cctcgtcgcg cccggctccg gcaaaaaggt ccttgcccat ccggaaggtt cgcccgagcc
     5821 gccgcaggca gagataattc gagcgaccct tcaccagcac cgccttgaac ccgtcgccga
     5881 gcaccttctg caggaagggg atatccttgt aaatcagctg ctcctggagg ctgatcgtgt
     5941 aggtggccac caccatgcgc tccctgcgct ccagcgaggc gtaaatcagc gggacaagat
     6001 acgcaaaact cttgccgact ccggttccgg cttcaacggc gaggtgcgac gatttatgaa
     6061 tcacaccggc cacggcacag gccatcgtcc gctgctgcgg ccgctcctcg tacgggaatt
     6121 cctccgcatg gcaggcctct tccagcaggc cgccgggacg gaaaaaggca tccgcgcggg
     6181 aaggaaaggt ctcttccccg cacgcctttt cgccggaccg gcctctggct ggtgaaatca
     6241 tcgctcctgc atatcagaaa acccggccga tggcggacaa aaaaagaaaa tattcggttc
     6301 ggaaatactt gattttctcc gcggcccgtg attagttaca cggcacaaac gaagcgagcg
     6361 tagctcagtg gtagagctcc accttgccaa ggtggatgtc gagggttcga atcccttcgc
     6421 tcgctccatt tttttatccc ggcacggccg gcccttcccc aaaagttcca atgcctggaa
     6481 gtttctgcgg aaaaagttcc aatgattgga agtttttgaa cgaaaagttc caatgattgg
     6541 aagttttgat cgggctggtg ggcgaggcgg gactcgaacc cgcaagcctt tcggcacaag
     6601 atcctaagtc ttgcgtgtct gccaatttca ccactcgccc ggaaaaaaga aaactggtgg
     6661 agggtaggag gttcgaactc ccgacctcct gaatgccatt caggcgctct cccaactgag
     6721 ctaacccccc accaatgaga aggtcgcgct caatataggg aaatatttca ggaagtcaac
     6781 cccctgagaa ttacaaatta ccgcacaacg aagcccagcc gcgcgaaggt gacatcgtcc
     6841 acctcggaat cgagcaggcg gcgctgatgg cttcggattt cgtattcacg cccggtttcc
     6901 atgccgaccg cggcatgcat ccagacccgc cgccagacgc gcatttccgc gccgagcgcc
     6961 cagcgccatg cctccacctt cagctcgtgg tcttcaaagt ccgtttcctc ctccagcttc
     7021 cagacattgc ccgcgggctg cagatcggtg aacagcacaa tgtcccgcga cagagcgcag
     7081 gatatccgcg gcgccggcag aaccatcgcg acacgccaca tcgggctcgc gtcccagatg
     7141 aagcccccca tgggatagag caggtcttcc ccgaattcgc ggtcatacgc cgcgcccaaa
     7201 gtcagctgcc agccctcgcg ccacttccac gtcgccaggg cggaggccga cagttcagca
     7261 tcggaaagcg aggcgtcctt catgtcgctt gccagttccg gggccagttc aacaatgatt
     7321 tcccaccggt cattcggcaa atacagcgca tcgagataca agacagcgga atagacgtcc
     7381 agatcgtcca gaggagagtc gtcgaattca aagcgcgaga ccgtcaggcc cgccccggaa
     7441 aaccactgca ggttcgggga ttcagcaaga ggcagccagg cggccatcga agcctccgac
     7501 caggccgcct cgccctcagt cccatcctcg ttgtcgaacg aggccgcatc cgcggccgtg
     7561 aagaggcccc acgcgaaggg atcctccggc acaatgctct ccatgcccca tgcggtcgcc
     7621 aatggaatca gaagaagcag cacggaagtt ttcatgactc actctccctg tttggacggg
     7681 cgaacagcct tcacatccat ctccagatta tcggttcccc tgaaactgtt caacttcatt
     7741 tggaaagcca cgtccagcgg gccatcggga agcggccggt cgccccagcc gaagaagatg
     7801 gcggggactg cagcttcgtt cacatcaagg ctgaatttaa ggtgcccgga gccgactgtg
     7861 cgaggagtgc cggctggcgc gagcccccgc aggccccaga gcggggccgg attgtccatt
     7921 ccaaacggct ccagcttctt ctggccctca taaagcgcag cgccgaattc cccgggctcc
     7981 agccaggcat caatggtgat ggagggacgc agatccgcat ggcgcagcgc aaccgatgcg
     8041 attcggttga actgctcccg gaattctccg atgcgggagg caggcagttc cagaccggcg
     8101 gccatggcat gtccgcccca tcgcgtcagc agggcggcgc aggcctgcag gttttccacc
     8161 atgttgaagc ccgggatgct cctgcacgat cccttcccga ccccgtcttc acccacggcg
     8221 atcaccacgg ccggacggtt gtatttgcgg accagccgcg acgcgacaat gccgaccacg
     8281 ccctggttcc agccctcgcc ggccgccaca agcgcatagt ccttcttccc gtcgaagctt
     8341 tcatcaagct gggcggacgc aaggctgaag atttccgttt cgatatcccg ccgctcccgg
     8401 ttggtccggt ccagatcacc cgccagccgc gcggcctccg ccgcatcccc ggtcataaga
     8461 agattcagcg cgtgcatggc ggtgcccagc cggccggcgg cattcaggcg cggcccaagc
     8521 tggaaaccaa gggcataggc ggtatagggc ggttttgttc cggccacttc cagcagcgcc
     8581 ttcaggccgg cattgcccgt gcgcgccaga cgttccagtc ccgcccgcac caggacccgg
     8641 ttgtcataaa gaagcggcac aatatcgcag accgtgccaa gggccacgag atccagatat
     8701 tcacgcagat caatatcaag cgccgatgat tctccggcct cgcggccttt cttgacgagc
     8761 gcgtggcaga gcttgtaggc cacccccacc ccggccagca gtttgctgcg aggatcatcc
     8821 cccagcttgg gattcaccag cgccagggcc ggagccacct cgccggatgc ctcatgatgg
     8881 tcggtgacca ccacatccac gccctgtgcc gcggcatatt caacggcctc gcgcgatccg
     8941 gttccgcagt ccaccgttac aatcaggccc ggcctgtact cgtccatgca gcgcgtcacg
     9001 ggttccatgc tcatgccgta gccgtcgtcc acgcgatgcg gcaggacggg atgcacctcg
     9061 gcgccgagcc gtgcaaggac tctgcagatc atggccgtgc tggtgacccc gtccacgtca
     9121 taatcgccat acaccaccac gggctgtttt tcgcgcagcg ccttccagat gcggtcaacc
     9181 gccggctcca tggccggcag gaggaacggg tcgccaagag aactgagcga cggagacagg
     9241 aactgcgccg tttcttccgc ggacgcccac ggccgagcgg ccagaacggc cgccaccgga
     9301 tggggaagcc cgtgagcatc cctgatagcc tttgccgcgg attcctgaaa agacgctgtt
     9361 ttccagactt tcaaactgct ctcctcaaca gaacaccccg cgcggcatgc cgcgcggggt
     9421 gtctcaactc aagaaacctg catcctacgc ggccttcggc ttgtcgcgat gccagagaag
     9481 catgatgggg gccgcgagga agacggacga ataggtgccc acaataatgc cgatcagaag
     9541 cgtgagcgcg aaatcaaaga tggcgccgcc gccgaagagc agcagaataa ccacgctcag
     9601 cagcgtggtc aaggaggtca gcaccgtccg gctcagcgtc tggttgatgc tgagattgac
     9661 gatatccttg tagctgcgtc cgcgcaccag cttgaggtcc tcgcgaatgc ggtcaaacac
     9721 gacaatggtg tcattcacgg aatagccgac gatcgtcagc agcgcagcca cgatcgggag
     9781 gctgatctgg cggccgagca ggcagtaaat tccgaccgtg atgagcacat catgcgcaag
     9841 ggccgcgatg gcgccaacgg cataggcaaa ctcaaagcgg atcgagatat aaaccacaat
     9901 gcccaccagc gaccagacca gcgccatgat gcccttgcgg cgcatttccg cgccgatctg
     9961 accgcccacc tgttcctcct gcaggagcgt ataaccggcg tatttttcct cgaacagatt
    10021 cttcaccgca tcgccgtcgt tctcgccgac gcgcacgtca agcatctcgt tgattcccgc
    10081 atcatcgtcc gcaatcaggt cggccttgta ctggagtccc gcatcaatgt tcagcgccgc
    10141 aagatcgccg cggatctgct ccacgggggc tttttcagtg aacccgtaca ggagcgaagt
    10201 gccgccggcg aaatccacgg cgaaattctt ctgcccgttc tgcacaaaca caaaccaggt
    10261 gccgaggata atggccagag aaagccccca cgtcgcatat ttcttcccga tgaaatccag
    10321 tttcgtgtcg cggaagaagg agaacatccg gagggatttc atcttgaagc cctcgaccat
    10381 gctgctgagc atgaggcggg tatagaccag cgccacgagc atgctcacga cgatgcccgc
    10441 gctcagcacg acggcaaagc cgcggacagg gcccgaacca aaggcgaaca ggatcaccgc
    10501 cgtcagcagc gtggtgatgt tggagtcaaa gatggttgtg aatgcccgct caaaaccggc
    10561 cgtgatggcg ctgggaaggc gtttggcaac acgaagctcc tcgcgtatgc gctcaaagat
    10621 cagcacgttg gcatccaccg ccatgcccag ggtgaggacc agcgctgcga tacccggcat
    10681 ggtcagcgtc ggcaggctga cggccgcccc cgtaaaacgc tgcccggaaa acaggcccag
    10741 gaaacccgag gccacaatca ggcccagcgg gagaagcagc aggtccagca gaagccccgc
    10801 attggccacc aggcccgccg cgcaataata aaccagcatg aacaccagca cagcggcaac
    10861 cgccacaacg gcggcacggc ttccgctgcg gatagagtca cggccgagcg acggatcaac
    10921 cgtgctggtc tgaatgatct tcaccggagc gggcagcgcg ccggcgcgca gcacattggc
    10981 cagccgcatg gcttccggca ccttgaagtt ccccgtgatt tcggcccggc cgttcgggat
    11041 ttccgtctga atgaccggcg cggaatacag cgtgccgtcc agaataatcg ccagctgccg
    11101 gcccaggtcg ctgtcgcggt tcttttcgcc gcgcggggca tagtcgcggg tcaccttggc
    11161 aaaacggcgg gcgccctgcc ggtcgaattc aagcgtgact cgcggggcgc cggtgacctg
    11221 atgataatcc acaccggcat tcttgatggt gtcgcccgtc atctggcggc ggatggagat
    11281 gtaatagggc ttgtagatgg ttcggccgga agcatcggtg tccttttcca gcatgaaggc
    11341 gcagccgggt ttcggctcga aacggcgcag ggtttcaagg aaggcctgat ccatctccgc
    11401 gtccttgacg gtctccgtat cgcgtatgta atagtctccg cgatcgctga tcttgaaccc
    11461 gcggggagcc ttgaagtcgc tgaacagctc ttccacccat tcggagcttt tctcatgaac
    11521 aaggcggaac tcaaggaaag ccacgctctc gatggactcg cgggcggcct cgcgcttttc
    11581 cttgtctatg ccggggagct gcaccacgat gcgctctgat ccctcggccg aagaggaata
    11641 aataacgggc tcggcaatgc ccaggccgtc cacgcggttg cgaatcactt ccagcgcatt
    11701 ttcccgggct tcggaagcgg cgcgggccac gcgggcggga agcgtatcgg gatcggcatc
    11761 cggttcgtca tcgaggatgc gcttgcggat ttcctctttg tcaatttcca cggtaaaact
    11821 ggtgccaccc tgcaggtcca ggcccagctt gaccttttgc gaaggaggat aaaccaccgc
    11881 aaaggatccg gccagaagca cgaccagcaa caaccatttc cacactgcat tcttttccat
    11941 gtctcgctac ctctctgctg ctctgtttaa atccgcgtga gccccctccc gggcgccatg
    12001 caggcggtta accgcttttt cgcgcatgaa ctgcacagct tcctgcacag catcacgatg
    12061 cgggacaggg actggccggg gcgcacccaa aatatttgcg agaacctagc aggcgcaggg
    12121 ctccaacgcc agcgaaatat tgggttctct gcggacccgg ggcgggcggt tatttgccaa
    12181 gcattctgcg cacagccgca gccaccgcct gatcggatgt ccgccttgcc gtccagccga
    12241 gcgcgcgcat tttatccacc gtgatgtgca ccctcggctg atctcccggc caggcctgtt
    12301 ttgttccttc tatctttatt tcagcgtctt caaggcccat ttcggttttg acgatctccg
    12361 cgatatccgt cacgcgggtg atggaatccg tcccgagatt gaaaaggtcg cacggtttat
    12421 cttccgtcat gggaatgttc cggaaggcat aggccatgcc gtcaatgcac tcctccacca
    12481 gaaaataatt cttctcctga ttgcccgcgc cgcggatcag caggctttgg ggattggccc
    12541 tgagtttgtg tatgaaatcg aaaatcacgc cgtgcgtcat gcgtgcgccg atgacatttc
    12601 caaaacgaaa catccacgcg cgcaaaccgt acagatggca gaaggaggaa ataaaggctt
    12661 cgctgccgat cttcccggcg gcataggtgg aaaccggcag aagcggaccc gaagcctctg
    12721 atgtggccac gtcaaagcaa aggtccccgt aaacagcgcc cgttgatgag aaaataacgg
    12781 gctgaatccc ggtcgcgcgc atggcctcga gcacgttgaa tgtgcccatc acgcagtcgc
    12841 gcaggtccct gcggggctgc tccacgccgc cgataatgtc cgtgttggcc gccagatgcc
    12901 ataccagatc atgcccggcc atcgccgcgg acagcttgtc catgtccagg atgtcagcct
    12961 catg
//
LOCUS       DAOQWR010000064        12921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5340_length_12921_cov_4.532800, whole genome shotgun sequence.
ACCESSION   DAOQWR010000064 DAOQWR010000000
VERSION     DAOQWR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5340_length_12921_cov_4.532800"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1293
                     /gene="pilM"
                     /locus_tag="PKK36_06545"
     CDS             265..1293
                     /gene="pilM"
                     /locus_tag="PKK36_06545"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNR94252.1"
                     /translation="MPAAEKKEAKSKRKQLPEVVALDMAGSGLKMVHARYGKDGFSVM
                     KIGELSAPPPPAEGEEVARLILPKEWTTYYAALLTTGSDAVIRYVTLPGHHPETDNLA
                     AQVREHMGIEDGYRVGYVHIKHPNVKTETRLLAAALKEEEAQQMMDRFASGQPAPCSL
                     EISSLAALNAFSEFGPLKNHPDDAVGFIDAGAQTTLMAFFNRGALSIVRKFNMGGDYL
                     VEHIQKEMGVDSEVARNIVCDGSFDISRSVSAVLGPFLRQLSISKDFIERRENCQLAA
                     IYLSGGMSLCGDWSRDIAQAAGVTVQLWNPLEDVTLEEGAVPEELVGQEPRFAAALGA
                     AWGVFQQS"
     gene            1290..1970
                     /locus_tag="PKK36_06550"
     CDS             1290..1970
                     /locus_tag="PKK36_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94253.1"
                     /translation="MSLRINLIKPGELRHSGAVRRSFLVQIAVAVVVCILVLGALFVV
                     IEARSTDRELQKLKAGLREVEKTYNEIKEVQQNLAYNNQVLDELSTWTRSRILWHEGL
                     MDVQALVPQDVRFTAFEVSSTLSLLDQKAPSSPDAAAPKVPPPPVPIRTYRLYLAGKV
                     THELANRVVVGFVEHLRREDRLGPLFQSVKLQRMDKNTEQGALANEHLFAVEALSHPR
                     QMLKEKAP"
     gene            1967..2611
                     /locus_tag="PKK36_06555"
     CDS             1967..2611
                     /locus_tag="PKK36_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94254.1"
                     /translation="MKFEELNKEQKQKVVLVIMGAAALLYVLYQFVVMPGISRMGKAK
                     DELEILQAEMEKIGHALSSGTRLRTDLEKSVEDLKISSTRYIVPADNPLAWAIERVYE
                     CADPLQVEIESVSPLMSTVPWERSAAAARLFNPYTVKVVTRCGYFTLTELIRLLETSN
                     PYVCVSDLRIKGTTDLMKHDVEFMLSWPKWADEKSGEKIRGLDGKPVLGSSAGI"
     gene            2668..4602
                     /locus_tag="PKK36_06560"
     CDS             2668..4602
                     /locus_tag="PKK36_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNR94255.1"
                     /translation="MKIFSFAVAVLAVVIMAVSPCRAQTAADDAASLDEALLDEILQD
                     AEPLQEEQLMTEEDMDALLDEAGLVDEEVPSGEVPVGEDIPAAQPANELSTDAQLTEE
                     IPAEEEPVEEAPMTGLGSEEVNIETELIELGLDDALSGTVDMGEETTDLITISLDDVP
                     VQDVVRMFARISGANIVAGTNLVGNVTVNLQDVEWQPALRVILDSVGLSMVEKTDGIY
                     SILSRSDLAAEPVTTETIFLNYTTVTNIIPVIRSMMVSSNASVSGFPSANAVIIQETV
                     ERMGPIKETIRRIDRPRSQVFIEAKFVELNDEAIKDIGINWQVLEGYKMGVSGLRWDY
                     QEDRDKLDRRSDTMTQHDTRSQVDILARGYDMYGAQLQDEEITFEEIPGTDPPAYASF
                     TEITPTRTRTDTIDIGRDIRQEMEDTFEKAVSDVRTAVFTADEFALTLSALKQNAGVQ
                     VVSNPKILVASGETASIHVGVREPNVRAVPQGDSGDRYAYELDTTNPFIDIGVKVDVT
                     PVVNTESNIAVRITPELSQSLAPLQFAGGLTFPRIQSRRVVTDFSLESGRTVAIGGLI
                     QTGDQEQVKKVPLLGDIPLIGKYLFRHTHTEKMQDEVIIFVTVSMAKADEIREESGIP
                     VEGRLIHKHMIMKRNALREF"
     gene            4636..5292
                     /locus_tag="PKK36_06565"
     CDS             4636..5292
                     /locus_tag="PKK36_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94256.1"
                     /translation="MKTRISKYTILAALFLGGIAFFVTGCDSEDSPDNGGLDNYFKNN
                     PYVSDPRIERDSYLTVTPSLANVGEVGQQVLFRVSGGSGGYTWDVSYPGRGTIQGEPA
                     SSDAIYTVRSVADNEVIVYDSVGHAGIAFISAAAASSSALAMTASSTQLNVDGAKCVL
                     TAVGGVPPYAWAVDDVARGNVSANAGNSVIYTRFNAGDNAVSLTDSAGTVLYIVIQQP
                     "
     gene            5435..>12921
                     /locus_tag="PKK36_06570"
     CDS             5435..>12921
                     /locus_tag="PKK36_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94257.1"
                     /translation="MKLMLNHPFLRAFPILIAVWLAACPAGYATYISGNVLVRSTWAD
                     DDANFNPPGDGDSTGDLLIGLWNAKGGAEGPPFTVSNLLIGAAMPFASGVSYNFTLTD
                     PVTGTLISDTYIVIAWVDANQNGAYDFGEPRNIPVSISVFNPSIGNTITVVDDSDEDS
                     MPDWWEAHWFADAVKPMAQQAFEDPDGDGLSNLQEYNISTLLPVMKYLNPANWDSDGD
                     GMDDKWEYTYYFQADAVGLNPTISNRFEDVDGDGLSSWQEYCGIDGDPLMVPDGYVDG
                     VYVGKRGTGDPDDLNPLDIDTDYDMLIDSFESAWYDTLNNIDPRDGVLAAPPEAPGSV
                     DSSIARQDSDNDGLSNYREMCLLAEFREGAVNGDKWVWSGTTPFPQQIFYTDDGDAVR
                     VCLMSSSGVNLNLGLDMEDVIENNRAELRNEEWTDPTDNTSYISDNNLLPGYDTDGDW
                     LPDGWEVQFNLDPRDPGDNSGTGWTHGPFGDPDGDGRGNWDEYLGQDGLRNATLRYIN
                     GTGDETNPNEYNHRPDSTYSWRWSSTNVPHAFITDPRPGTGINRYETLGSALPTASLG
                     VDIGMDSDDDGIPDELEINPAAGDASSPVHSCDPYLPQSALITSAAGILIPDPEPAFA
                     IGLFPAGIREDLQRRDWTIECQVKLLSDSMSGNLFNFQTVRGPSPRTVYRLSLANNVP
                     SLTAQASDGTFYTVTANALPTNEWIHVAGVWDRANNSLALYIQGVLYQGRTAVDDMTS
                     SLMFPATNVLALAASADGSFVNNLLIDEVRIWGIARPPEKISEYASKLTPQARGDDVW
                     ISSTGNRYYDINDTLLVHGGSLYEGQPGVKLSNVVVHLQTYWLDDGDRQYEQDSDILL
                     THLQNQVSEGTIGIPVANVYYADKDGSGDYTRQSLLAYYRFDDGGTTAEDFSRKAKNG
                     LLGAVREDMLFGDMGYALPTAGFSWDNDAAPVVGSQPNAADDTDGDGLPDAWELLHHL
                     NPYDNGYGGQSAAGKLDGPFGAYGDPDDDGLYNIYEYWAGTNPRSDDSDGNGIPDTQE
                     DADGDNVSNLTEQKLGSRPDLVDTDDDGMTDSEEQQAGSNPADPGDPPVSRALTFGGT
                     MDDYIQVPGSYAQRLTDWTLEAFINPDSVAAGEGVVVRRAVQNIGAGVYALNYLLGTE
                     SDGVGGLRAYAGYVMANGTEYILRGGSIPAGDWTQVSATYDNLSATLVLYVDGVAVTN
                     TSLFDVVPPINGKGGEYFLRIGEDFGGMLDEIRIWNYARTEEQVVASYDETVLRTSDG
                     LVHYFRLDDGQAAVDPAGPQDWTWPNDWNEMWKHSAVVHGSVAFVEPGAIVPPATLRV
                     ILQPASAVAEGAAWSFDGGAWQDSGTTMYEVPAGIHDLLYKTIEGWTAPTTEQVNLLS
                     GTATTLTRTYIRNGAIMIRLDPEDAVAAGAAWRVDGGDWQLDEAIVENLSPGSHNIEY
                     LSIEGWVEPAGETVTVTSGATNTLTRSYVQSTGWLLVNLSPADAVTEGAQWRADTGTW
                     RDSGFLLNISVGSHYVDYRDVTGWIAPLGETVSILNNSTTTINRVYVMDASADLDLDG
                     MLDTWEVTWWGDLSAVPTEDDDNDGLSNLEEFTISQRFPALANISPVDFDTDADGMDD
                     HFEYRYFANGFGLNATVDDAAADADGDGLMNISEYLGMDGIAPMVQDPVEDPGVASDT
                     VMSTDELNPLDIDTDGDSLIDSFEAAWAGAPVYIPESVVNLDDGLTDYREQCLLTELR
                     EGGPNDIWTLGSNSMPVADANGIRAFVPPLMLGATLGTTITDNLVPLRGQEWTNPHDA
                     DTDGDSLPDGWEVEFGIDPRNASGANGFAGDPDGDGLINAEEYFGQDGRRSTTRMFVN
                     GTGDETNPNQYSHRPDSTYEWRWSVNWLLYGFSSSTPGGDGNSRIETLGGALPTASLG
                     SDYGADTDDDGIPDGLEIHPGTGELPSSPVHSCDPFIRRSALIADAAGIAIPDPEPSA
                     SGVREDLQRRDWTIECHVKLLSDSMSGHLINFETEIGGVSRIVYRLSLSNNVPTLVAA
                     NSAGSVYAISANRLPTNKWIHIAGVWDSENNNLALYIHGVLHMGQMVYGESLSALQYP
                     ATNELALAVSPDGSFVNNLLLDEVRIWGLARTEDQIAHYARRLPLPAVGDDAWIDEAD
                     EGTGRAYIYRSAHADQLLVNGGAMFEGQPGMLATNALVSFKYVPAQVKGIYSPGEDVW
                     LDDGDFIYESERDILLLDGGTLTEGEAGAVLTHYLGSDNPASVYWSDKDGDGKFTHNG
                     LLACYRFDDGGTTAEDFARRAKNSLYHPNTENYTFGDFGYALPTNNFLWVTNDYAPVQ
                     GVEEYGADDSDGDGMADGWEIANNLSPYDNGTGHETVPGKANGPEGPMGDQDGDGLRN
                     LYEFWAGTNPRASDSDQDGILDINEDLDGDGVANVVEQALSSRPDKADTDDDGLTDNL
                     EQARGSNPAKSTDPQVSRAISLGGLPQDYLDIPLGPYQGLTAWTLEAWVNPRTNISES
                     SGTVVRRVVETSDGGT"
BASE COUNT         2838 a         3429 c         3921 g         2733 t
ORIGIN      
        1 gaatgggtag agaaagctca ctcatttttt cctgaatctt gatgaataat tcaggttaga
       61 ggtatttttc tgactcaaga tacagaattg gactgggtta taaagtgggg tcatgcggtt
      121 ttcaaggcgt atgagaaaag ttattaacag gcgccgtttt ctgtggtaat ctgggttttg
      181 aatatttgag aagagaacca aagcaggccg cttccgtgca ggcggcatgt ttgggtattt
      241 tgtgctgtgg ctttgaggtg aagtgtgccc gctgctgaaa aaaaagaagc gaaaagcaag
      301 agaaaacagc ttcccgaagt ggtcgcgctc gatatggcag gcagcggcct gaagatggtt
      361 cacgcccgct atgggaaaga cggcttttcc gtgatgaaaa taggagaact gtcggccccg
      421 ccgccgccag ccgagggcga ggaagtggcc agactgattc ttcccaagga gtggaccacc
      481 tattatgcgg cgctgctgac cacaggatct gatgcggtca tccgttatgt gaccttgccg
      541 ggacatcatc ccgagactga caatttggcg gcgcaagtcc gagagcacat gggcatagaa
      601 gatggatacc gggtaggcta cgttcatatc aagcatccca atgtgaagac cgaaacccgc
      661 ctgctggccg cggcgctgaa ggaagaggag gcgcagcaga tgatggaccg atttgcttcc
      721 ggtcagcctg cgccctgttc tctggagatc tcaagtctcg cggcgctgaa tgcgttttca
      781 gagttcgggc cgctgaagaa ccatccggat gacgccgtcg gattcataga tgcgggtgcc
      841 cagaccacgt tgatggcatt tttcaaccgc ggggctctct ccattgttcg caagttcaac
      901 atggggggag attacctggt cgagcatatt cagaaggaga tgggcgttga tagtgaggtg
      961 gcgcgcaata ttgtgtgtga cggatccttt gacatttcca ggtccgtaag cgccgttctg
     1021 ggcccgttcc tgaggcagct ttctatctcc aaggatttca ttgagcgtcg cgagaattgc
     1081 cagcttgccg caatttatct ctcgggcggc atgagtctgt gcggcgactg gagcagagat
     1141 atcgcgcagg cggcgggggt gacggttcag ctttggaatc ccctggagga cgtgaccctg
     1201 gaggaaggcg ctgttcccga ggaattggtc gggcaggagc cgcggtttgc cgccgcgctg
     1261 ggcgccgcat ggggagtgtt tcagcaatca tgagtctccg cataaatttg atcaaacccg
     1321 gggagttgcg tcattccggc gccgttcgga gaagtttcct ggttcagatc gcggttgccg
     1381 tggtggtttg cattctggta ctgggcgcgc tgtttgtcgt cattgaggca cgcagcacgg
     1441 accgggaact ccagaagctc aaggccggcc ttcgggaagt tgagaaaacc tacaatgaaa
     1501 tcaaggaggt ccagcagaat ctggcatata acaatcaggt tctggatgag ctcagtacgt
     1561 ggacccgttc cagaattctc tggcatgagg gcctgatgga cgtgcaggcc ctagtgcccc
     1621 aggatgttcg atttacagca tttgaagtca gcagcaccct ttccctgctg gatcagaaag
     1681 ccccgtcttc tcccgatgcg gccgccccca aggtgccccc gccgcctgtt ccgatccgca
     1741 catatcggct ttatcttgcc ggcaaagtca cacatgagct ggccaacaga gtggtggtgg
     1801 ggtttgttga gcatcttcgc cgcgaggaca ggctgggtcc gctgtttcag tctgtcaagc
     1861 ttcagcgcat ggataaaaac acggagcagg gtgcgctggc caatgaacat ttgtttgcag
     1921 tggaagccct gagtcatccg cggcagatgt tgaaagaaaa agcgccatga aattcgagga
     1981 actgaacaag gaacagaagc agaaggttgt gctggtcatc atgggcgccg cagccctgct
     2041 gtatgtgctg tatcagtttg ttgtgatgcc gggaatctcc cgaatgggga aagccaagga
     2101 cgagctggaa atcctgcagg cggaaatgga aaagatcgga catgcgcttt ccagcggcac
     2161 gcggctgcgc acggacctcg aaaagtccgt ggaagacctg aagatcagca gcactagata
     2221 tattgttccg gccgacaatc ctctggcctg ggccatcgag cgtgtctatg agtgcgcgga
     2281 tccgctgcag gttgaaatag agtccgtgtc accactgatg agcactgtcc cgtgggagcg
     2341 ctcggcagcg gctgcacggc tgttcaatcc ctataccgtc aaggtggtca cccgctgcgg
     2401 atatttcacc ctgacggagc tgattcggct gctggaaact tccaatccgt atgtatgcgt
     2461 gtcagatctg cgtatcaagg gcacaacgga cctcatgaag catgatgtcg agtttatgct
     2521 gtcatggccc aagtgggccg atgaaaagag tggagagaag attcggggtc tggacgggaa
     2581 gcctgtactc gggtcgtcgg cgggtattta atgtttgaaa aaggatgtcc tgttatgtgt
     2641 gacaaaaaca agatcaagga gccgggaatg aaaatattct cctttgcagt agccgtgctg
     2701 gctgtggtga taatggcggt tagtccctgc cgggcgcaaa ccgcggctga cgatgccgcg
     2761 tcgctggatg aagccctgct ggatgagatt ctgcaggatg ccgaacccct gcaggaagag
     2821 cagctcatga ccgaagagga catggacgct cttcttgacg aggccggact tgttgatgag
     2881 gaagtcccgt ccggagaagt gcccgtcggg gaggatatcc ccgccgcaca gccggcgaat
     2941 gaactgtcca cggatgcaca gctcacagaa gaaattccgg cggaagagga accagttgaa
     3001 gaagctccca tgaccggttt ggggtcagaa gaggtgaaca ttgagaccga gttgatcgag
     3061 ctcggactgg atgatgccct ctccggcact gtcgacatgg gggaggagac aacagatctg
     3121 atcaccatat ccctggatga tgtccctgtt caggatgtgg tgcggatgtt tgcgcgaatt
     3181 tccggcgcca acattgtagc gggaacaaat ctcgtgggaa atgtcacggt taatctgcag
     3241 gatgttgaat ggcagccggc gcttcgcgtc atccttgact ccgttggtct ttccatggtg
     3301 gaaaaaacag acgggatcta cagcattcta tcacgcagcg accttgcggc tgaaccggtc
     3361 accacagaga ctattttcct caattatacc acggtcacca atatcatccc ggtcatccgg
     3421 agcatgatgg tgtcctccaa tgcgtcggtt tcgggtttcc cttcggcgaa tgcagtcatt
     3481 attcaggaga cggtggagcg catggggccc atcaaggaaa caattcggcg gatcgaccgg
     3541 ccccgctccc aggttttcat cgaggcaaaa ttcgttgagc tcaacgacga ggccatcaag
     3601 gatatcggca tcaactggca ggtgctggag ggctacaaga tgggcgtcag cggcctgcgt
     3661 tgggattatc aggaggatcg tgacaagctc gaccggcgca gcgacacaat gactcagcac
     3721 gacacccgtt cgcaggttga tatccttgcg cggggttacg acatgtacgg ggcgcagctg
     3781 caggacgagg aaattacatt tgaggaaatt cccggaactg atcctcccgc ctatgcttct
     3841 ttcacggaaa taacacccac ccggacgcgg acagatacca tcgatattgg tcgcgacatt
     3901 cgccaggaga tggaggacac ctttgaaaaa gcggtcagcg atgtgcgcac cgcggttttc
     3961 actgcggatg agtttgcgct gacactcagt gccctgaaac agaatgcggg ggtgcaggtg
     4021 gtatccaatc cgaaaatcct ggtggccagc ggcgagaccg catccattca tgtaggggtc
     4081 cgcgaaccga acgtgcgcgc tgttccgcag ggcgacagcg gggaccgcta tgcctacgag
     4141 cttgatacca ccaacccgtt catagatatc ggcgttaagg ttgatgtaac cccggtggta
     4201 aacaccgaga gcaacattgc tgtccggatc acgccggaat taagccagtc tctggctccg
     4261 ctccagtttg cgggtggact gacattcccc cgcatccaga gccgccgggt ggtcaccgac
     4321 ttcagccttg aaagcggtcg caccgtggcg atcggaggcc tgatccagac tggcgatcag
     4381 gagcaggtca aaaaggttcc gctgctgggc gacatccccc tgattggaaa gtacctcttc
     4441 cgtcacacac ataccgaaaa aatgcaggat gaggttatta tcttcgtgac ggtgagcatg
     4501 gccaaggccg atgagatacg cgaagagagc ggcattcccg ttgagggccg tctgattcac
     4561 aagcacatga tcatgaagcg caatgcgctt cgggagtttt aacgcgatcc ggggaatatg
     4621 aaggagttca tgacaatgaa aacacgtata tcgaaataca cgatcctggc agctctgttt
     4681 cttggcggca tcgcgttctt tgtaacagga tgcgacagcg aggacagtcc ggacaacggc
     4741 gggcttgaca actatttcaa gaacaatccg tatgtgtccg atccgcgaat tgagcgcgac
     4801 agctatctga cggtaactcc gtcgctggcc aatgtagggg aagtggggca gcaggtgctg
     4861 tttcgtgtgt cgggcgggtc cggaggctac acatgggatg tttcttatcc cggccgcggc
     4921 accattcagg gtgaacctgc ttccagcgac gcgatatata cggttcgcag tgttgcggac
     4981 aacgaggtta ttgtatatga ctccgttggg catgccggta ttgcctttat atccgccgcc
     5041 gccgccagca gttccgcttt ggccatgacg gccagcagta cgcaactcaa tgtggacggg
     5101 gctaaatgtg tgctgaccgc tgtgggcggc gttcccccat atgcctgggc cgttgacgat
     5161 gtggcacggg gcaatgtaag tgccaatgca ggaaacagtg tgatttatac gcgctttaat
     5221 gcgggagaca acgcggtgtc cctgacagac agcgctggca cggttttata tattgtaatt
     5281 cagcagccat aactggctga ttacgggtat attgatgctg aagggcatgg tgcacaaaac
     5341 gcacctgtcc tcggctgttt gaatgtgcag agatgatttt gtaatggata atgtttggaa
     5401 aaaactgcct gtgcggcgga ctgggggttc agcaatgaaa ctcatgttaa atcatccttt
     5461 tcttcgtgcc ttcccgattc tcattgctgt atggctggcc gcctgcccgg ccggctatgc
     5521 gacctatatt tcaggaaacg tccttgtccg gtccacctgg gccgatgatg atgccaactt
     5581 caatccgccg ggggacgggg acagcaccgg cgatctgctc attggtctct ggaatgccaa
     5641 gggcggtgcg gaaggcccgc cgttcacggt ttccaacctg ctcattggag cagccatgcc
     5701 gtttgcatcg ggtgtttcct ataacttcac cctgacggat ccggtgacgg gcacgctcat
     5761 cagcgacacc tacattgtga tcgcctgggt ggatgccaac cagaacggag catatgattt
     5821 tggcgaacct cgcaacatcc ccgtgtccat ttcggtcttc aatccgtcca ttggaaacac
     5881 cattacggtg gtcgatgact cggacgagga cagcatgcct gactggtggg aggctcactg
     5941 gttcgcggat gccgtcaagc ctatggcgca gcaggcgttt gaagatcccg acggcgacgg
     6001 attgagcaat ttgcaggaat acaatatcag cacactgctg cctgtgatga aatacctcaa
     6061 tccggcgaac tgggattccg acggcgacgg aatggacgac aagtgggaat acacctatta
     6121 cttccaggcg gacgcggtag gtctcaatcc cacgatttcc aatcgttttg aggatgtgga
     6181 cggcgacggg ctttcaagct ggcaggaata ctgcggcatt gacggcgatc cgctgatggt
     6241 cccggacggc tatgtggacg gggtgtacgt tggaaagcgg ggcacaggtg atccggatga
     6301 cctgaacccg ctggatattg acaccgatta cgatatgctc atagacagct ttgagtccgc
     6361 atggtatgac acgcttaata atattgatcc tcgcgacggg gtgctggccg cccccccgga
     6421 agcgcccggc agcgtggatt cctccatcgc gcggcaggat tctgacaacg acggcctgag
     6481 taattatcgc gagatgtgcc tgctggccga attccgcgag ggtgccgtca acggggacaa
     6541 gtgggtttgg tctggaacga cccccttccc gcagcagata ttctacacgg acgacggtga
     6601 tgccgtgcgc gtgtgtctga tgagctcgtc cggcgtgaat ctgaatctcg ggctcgacat
     6661 ggaggatgtc attgagaaca accgcgcgga actgcgcaac gaggaatgga cagatcccac
     6721 ggacaatacc agttatatca gtgacaacaa tctgcttccc ggttacgaca cggacggcga
     6781 ctggcttccg gacggctggg aagtgcagtt caatttggat ccgcgtgatc ccggagacaa
     6841 ttccggaacc ggctggaccc acggtccgtt tggcgatcct gacggcgacg ggcgaggcaa
     6901 ctgggatgaa tatcttggcc aggacggctt gcgcaatgcc accctccggt atatcaacgg
     6961 caccggggat gaaaccaatc cgaatgaata caatcaccgg cccgattcca cctattcctg
     7021 gcggtggtct tccaccaacg tgccgcatgc ctttattaca gatcctcgcc cgggaaccgg
     7081 catcaaccgc tatgaaacac tggggtcagc ccttccgacg gcatcacttg gagtggacat
     7141 cggtatggac tcggacgacg acgggattcc cgatgagctg gaaatcaacc cggccgctgg
     7201 ggatgcatcc agcccggtac attcctgtga cccttatttg ccgcagtcgg cccttataac
     7261 ctctgccgcg ggcatactga tcccggatcc cgaacccgcc ttcgccatag gccttttccc
     7321 cgccggtatt cgcgaggacc tccagcggcg tgactggacg atcgaatgcc aggtgaaact
     7381 gctgagtgac agcatgtccg gcaacctgtt caacttccaa accgtccggg gcccgtcacc
     7441 ccgcacggtg tacaggcttt cgctggccaa caatgtcccg tcactgaccg cgcaggccag
     7501 tgatggaacc ttttacacgg ttacggccaa tgccctgccc accaacgaat ggattcacgt
     7561 ggcgggggtc tgggaccgcg ccaacaacag ccttgctctg tacatccagg gtgttcttta
     7621 tcagggacgg accgcggttg acgatatgac atccagcctg atgttcccgg ccactaatgt
     7681 gctggcactg gcggcctcgg cggacggttc ctttgtcaac aatctgctca ttgatgaagt
     7741 gcggatctgg ggcattgccc gtccaccgga aaaaatttcg gaatatgcct caaagctcac
     7801 ccctcaggcg cgcggtgatg atgtctggat ttcaagtacc ggcaaccgct actatgacat
     7861 caacgacacg ttgctggtgc atggcggatc gctttacgag ggccagcccg gcgtgaaact
     7921 gtccaatgtt gttgtccatc tccagacgta ctggcttgac gatggcgacc gccagtatga
     7981 acaggacagc gacattcttc tgacgcatct ccagaatcag gtctcagagg gaaccatagg
     8041 cattccggtt gcaaacgttt actacgccga caaggacgga agcggagact atacccgcca
     8101 gtctcttctg gcttattacc gctttgatga cggcggcacc accgccgagg acttttcgcg
     8161 caaggccaag aacgggctgc tcggggcggt gcgcgaagac atgctgtttg gcgacatggg
     8221 gtatgccctc ccgaccgccg gtttttcgtg ggacaacgac gcagcccccg ttgtggggtc
     8281 gcagcccaat gccgcagatg acaccgacgg cgacggactt cccgatgcgt gggaactgct
     8341 tcatcatctc aacccctatg acaacggcta cggcggccag agcgccgcgg ggaaactgga
     8401 cggacccttt ggcgcatacg gcgatccgga tgacgacgga ctgtacaata tttacgaata
     8461 ttgggctggc accaatcccc ggtcggatga ctcggacggg aatggaattc ccgacacgca
     8521 ggaggatgcc gatggcgaca acgtttccaa tctgacagag cagaaactcg gctcgcggcc
     8581 cgacctggtg gacacggatg atgatggaat gaccgacagc gaagagcagc aggctggatc
     8641 caaccctgcg gatcccggag atcctccggt ttcccgtgca ttgacctttg gcgggacaat
     8701 ggatgattac attcaggtgc ctggaagtta tgctcaaagg ctcacggact ggacgctgga
     8761 ggcttttatc aatccggaca gcgtggctgc cggtgaaggc gttgtggtcc ggcgcgccgt
     8821 tcagaatatc ggggccggtg tgtacgcgct gaattacctt ctcggaacag aatcagatgg
     8881 agtcggcggc ctgcgggctt acgcaggtta tgtaatggct aacggcactg aatacatact
     8941 tcgcggcgga tcaattcctg ccggggactg gacacaggtg tcggcaacct atgacaatct
     9001 ttccgccaca ctggttttgt atgtggatgg cgttgccgtg acaaacacca gcctttttga
     9061 tgttgtcccg ccgatcaacg gcaagggcgg ggaatacttc ctgcgtattg gcgaggactt
     9121 cggcggaatg ctcgacgaaa tccgcatctg gaattatgcc cgcacagaag agcaggttgt
     9181 ggccagttac gatgagacag tgctcaggac ttctgacggt cttgtgcatt acttccgcct
     9241 tgacgacggt caggcggcag ttgatcccgc cggtccgcag gactggacgt ggccgaatga
     9301 ctggaatgag atgtggaaac attccgctgt agtgcacggc agcgtggcct ttgtagaacc
     9361 cggggccatt gttccgcccg ccacgctccg ggtgatcctg cagccggctt cggcggttgc
     9421 ggaaggcgcg gcatggtctt ttgacggcgg ggcctggcag gatagcggca cgaccatgta
     9481 cgaagttccc gcaggaatcc acgatcttct ttacaagacc attgagggct ggacagcgcc
     9541 gaccacagag caggttaatc ttttgagcgg aacagcaacc acgctgacgc gcacctacat
     9601 caggaacggg gccatcatga tcaggctcga tcccgaagat gcggttgctg ccggggcggc
     9661 gtggcgcgtg gatggaggag actggcagct ggatgaagcc atcgttgaga acctgtcgcc
     9721 cggttcacac aatattgaat atctgagcat agaaggatgg gtggagcctg ccggcgagac
     9781 cgtaaccgtg acttcgggcg cgaccaatac gctgaccaga agctatgtcc aaagcaccgg
     9841 ctggctgctt gtgaaccttt cccctgccga tgcggtgacg gagggggccc agtggcgcgc
     9901 cgataccgga acctggcggg acagcggatt tcttctgaat atttcggtcg gatcgcacta
     9961 cgtggattac agggatgtga ccgggtggat cgctccgctg ggtgagacgg tgagcattct
    10021 caacaacagc acgaccacga tcaaccgggt atatgtcatg gatgcaagtg cggatctgga
    10081 tctggacggc atgctggaca cctgggaggt gacatggtgg ggcgatctca gtgccgttcc
    10141 gacagaagac gatgataacg acgggctcag caatctggag gagtttacca tttcccagag
    10201 gttcccggca cttgcgaata tcagtccggt ggactttgac actgatgccg acggcatgga
    10261 tgaccacttc gaatatcgct atttcgccaa cgggtttggc ttgaatgcca ccgtggacga
    10321 cgccgccgct gacgccgatg gcgatggcct gatgaacata tccgaatatc tgggcatgga
    10381 tggcattgcc cccatggtgc aggatccggt cgaagatccg ggggttgcct cggataccgt
    10441 tatgagcaca gatgagctga atccgctgga tattgataca gacggtgact cgcttattga
    10501 cagctttgag gcggcctggg ccggcgcacc ggtttatatt cccgagtctg tcgttaattt
    10561 ggacgacggg ctgacggatt atcgtgaaca gtgcctgctg acggaactcc gtgaaggcgg
    10621 ccccaatgat atctggacgc tggggagcaa cagcatgccg gttgcggatg ccaatggcat
    10681 tcgcgctttc gtgcctcccc tgatgctggg agccacgctg ggcacgacca taactgacaa
    10741 tctggtgccg ctgcgcggcc aggaatggac taatccccat gacgcggaca cagacgggga
    10801 cagtctgccg gacggctggg aggttgaatt tgggattgac ccgaggaatg cttccggggc
    10861 gaatggcttc gccggcgatc cggacggaga cggtctgatc aacgccgagg aatattttgg
    10921 gcaggacggc cgtcgttcca ctacgcgaat gttcgtcaac ggaacggggg atgaaaccaa
    10981 tcccaaccag tacagccacc gccccgattc cacctacgag tggcgctgga gtgtcaactg
    11041 gcttctttac ggattctctt ccagcactcc gggaggcgat ggaaacagcc gcattgaaac
    11101 tctgggcggg gctctgccca cagcctcgct cggcagtgat tatggcgccg acaccgacga
    11161 cgacgggatc ccggacgggc ttgaaataca tccgggcaca ggagaactcc cgagcagtcc
    11221 ggtgcattcc tgcgatccgt ttattcgacg gtcagctctg attgccgatg cggcgggtat
    11281 tgccatcccg gatcctgaac cttctgcatc aggagtgcgc gaagaccttc agcgcagaga
    11341 ctggacgatc gaatgccatg tgaagcttct gtcggacagc atgagcgggc atttgatcaa
    11401 ctttgagacg gaaatcggcg gtgtttcgcg gattgtctac cggctctctc tttccaacaa
    11461 cgtgccgacg cttgttgccg ccaacagtgc gggttccgtt tacgccattt cggccaaccg
    11521 gctgccgacg aacaaatgga ttcacatcgc cggcgtatgg gacagtgaaa acaacaatct
    11581 cgcgctctat atccacggcg ttctgcatat gggacagatg gtttacggcg agtcgctgtc
    11641 cgcgctgcag tatccggcca ccaatgaact ggcgctggcc gtttcgccgg acggatcgtt
    11701 tgtaaacaat ctgctgctgg acgaagtccg catctgggga ctggcccgca cagaggatca
    11761 gattgcgcat tatgcccgca gactgccgct gcccgcggtg ggggacgacg cgtggattga
    11821 tgaggccgat gagggaacgg gccgtgctta tatctatcgt tctgcccatg ccgatcagct
    11881 gctcgtgaac ggcggggcca tgtttgaggg ccagccgggc atgctggcca cgaatgccct
    11941 ggtcagcttc aaatatgtgc ccgcccaggt gaaaggcatc tattccccgg gtgaggatgt
    12001 ctggcttgat gacggtgact tcatctatga gtcggagcgt gacattctcc tgctggacgg
    12061 aggcacgctg acagagggcg aggcgggggc cgtgctgacc cactatctgg gttccgacaa
    12121 tcccgcaagt gtgtactggt ccgacaaaga cggggacggg aaattcacgc acaacggtct
    12181 tctggcctgt taccgctttg atgacggcgg tacaacggcc gaggattttg cgcgcagggc
    12241 gaagaacagc ctttatcatc cgaacaccga gaactacacc tttggcgatt tcggatatgc
    12301 cctgcccaca aacaacttcc tctgggtaac caacgactat gctccggtgc agggcgttga
    12361 agaatatggc gctgacgatt ccgacggcga cggcatggcg gatggatggg aaatagccaa
    12421 taatctcagc ccgtatgata acgggaccgg ccatgaaaca gtgcccggaa aagccaacgg
    12481 acctgaagga cctatgggtg atcaggacgg cgacggcctg cgtaatctgt acgaattctg
    12541 ggccgggacc aatccccgtg cctcggattc tgatcaggac ggaatcctgg acatcaacga
    12601 ggatctggac ggcgacggcg tggccaatgt ggtggaacag gcattgagca gccgcccgga
    12661 caaagcggat accgatgatg acgggctgac agataatctg gaacaggcaa gggggagcaa
    12721 ccctgccaag tccaccgatc cgcaagtttc ccgggccatt tctctgggcg gactgccgca
    12781 ggactatctt gatattcctc ttggacccta tcagggcctg accgcatgga cgcttgaagc
    12841 atgggtgaat ccccgtacca atatttcgga atcaagcgga acggttgtgc gcagggtggt
    12901 ggaaacctcc gatggcggga c
//
LOCUS       DAOQWR010000065        12914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5349_length_12914_cov_5.469554, whole genome shotgun sequence.
ACCESSION   DAOQWR010000065 DAOQWR010000000
VERSION     DAOQWR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5349_length_12914_cov_5.469554"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PKK36_06575"
     CDS             <1..2055
                     /locus_tag="PKK36_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94258.1"
                     /translation="TPRAVKAFRSLQSAITNEIEAALNLLDWSGVLIDLGQRGDASVI
                     LSQLLTDYPASPQAVPGSLWLSRIHLEEGRLPEAEKLLLDLMARENLADDSRAEACRV
                     LAAVAEAQTNYTRAVEFLDRAVKLSADPVVLNQSRLRKARLLIRAGRRDEGEALLHEW
                     IGANTANPHAPESLLTWADELFASGEYEKAYREYQYYQEVFSDTPGRFRAQMGKGWCL
                     FGLKRHAEAVREFERALSLAEGDADRKQALFKIADALYADGEYTAARAKYLEVTKLFP
                     AAQEAPQALYQAAECYAEAGQYDAAEKEFHAIEDAYPESGFARQASLRIAAMKELIGE
                     WEQAVSQYNRILREFPGSLHAGRARHRRGMVRYRLGSFPGALADFEKVVQEFPGHDFS
                     EQAFYMRGWTHYLMGQSDKAQDICREFVQKFPDSAWAPDVLFWLAEYQFNNGFHQKAE
                     AGFTGVALRYPSSELADDAIFWAGQSAVLQREYLRAIEHFSKLVQNYPESPRLVQARF
                     AQGDALSELGQFAAAILAYEEVILKAQGSYTAALARGRIGDCQFTLGAGDPARYAEAI
                     KSYAALTGRADVPVEISLQAEYKLGRCLDKTGDAAGALERYLNVIYRYVAQKNRAVGS
                     EAWFTRAAFGAAAIREQEQQWSEALRIYERIIEEQVPAAEEARMRIRKLRTERWVTPG
                     TD"
     gene            2070..2690
                     /locus_tag="PKK36_06580"
     CDS             2070..2690
                     /locus_tag="PKK36_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR94259.1"
                     /translation="MQEMINAGGPMVWVILAAGVLAATVFVERLFHLHRAQIKSDDFL
                     KGIYNILNRGNIVEAVSICEDTPGPVADVVRAAILNHDAAVPEMEKAVQEAGLLEIPR
                     LERNISTLATVAQLAPLLGLLGTVLGLMQNLLVIQQKAPLVQPGDLAGGLWQALISTA
                     AGLTLAIPAHAAYNLLVSRVESILLDMERASVEIIAYLSKKGAGPR"
     gene            2690..3145
                     /locus_tag="PKK36_06585"
     CDS             2690..3145
                     /locus_tag="PKK36_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR94260.1"
                     /translation="MRSGVYLRLSKLHRLYAARNRIRRGPPGLIPLLDLVLILFVFYM
                     AHAPFVLQPGITMTLPSSPFRDGVQFGRLLVTVTRDGVIFFNDDRVSLQELPARFRNA
                     ADDESLIIQADGFVSHHMLVQIYNMASDAGIREVVLAAREPGVKGGVPR"
     gene            3142..3876
                     /locus_tag="PKK36_06590"
     CDS             3142..3876
                     /locus_tag="PKK36_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94261.1"
                     /translation="MKMSAERCAADRRIRAIAAAGSCLWVGLFAVLFRPAAPLAYAAV
                     SPSARLLYYPMLDEGGSGFVSPGLWSPRLFSFPSSLGFSAFLETRPNDLRPRLEPLET
                     ESEFLAYSAPETARIPEAGGLSAQVRAFFASQPSPYRSLPQAPSGSVFDVRFLEGLSE
                     EDFMDISLPAALSSGEAGWQAQLEIEVQPDGTVSHVFLMNESQLRGNRNLLVQAARKW
                     RTVPSDKVRRGCVTVRSVPPSPGVRP"
     gene            3873..4109
                     /locus_tag="PKK36_06595"
     CDS             3873..4109
                     /locus_tag="PKK36_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94262.1"
                     /translation="MIQVSWPVIFYVPLALNLLVVSLIGLFYFIRRYEPHVRYANERI
                     YRCAACGHVYVDSRKMPLLRCPRCDSLNEAVRTH"
     gene            4142..5458
                     /gene="hisD"
                     /locus_tag="PKK36_06600"
     CDS             4142..5458
                     /gene="hisD"
                     /locus_tag="PKK36_06600"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNR94263.1"
                     /translation="MVKNAKLDIKSVRWTVTSQPAEVEEFLQRPAFDPAAEKAAARML
                     AAIRSRGDEAVLEFIEKHDGVKLSAGALCVTPEEREGARAAVDDDFKRAAREARRRIS
                     RFAEAGIRKDWSITSPKGGMIGERFVPLDRVGVYIPGGAAPLASTAMMTIVLARAAGV
                     PEVVACTPAGRDGTINPALLYAIDFAGATEIYRIGGIQAIGAMAYGTSTVRKVQKIVG
                     PGGPYVTAAKRQVYGEVSLDMVAGPSEIAVLADDSISPSFVAADLLSQAEHGSGAEKV
                     LLVTPSERLADGVARELLVQSETLPRRDFVRKVLQHGMLIVVTDNLDAGMEVCNRFAP
                     EHFELLVQEPRRWIQKVRAAGAVFAGPWTPESAGDFAIGPSHVLPTGGTAAFFSGLTV
                     DDFQRRMSVMSLTRADLIELLPVIEAFGRVEGLEAHARSARIRFES"
     gene            5480..6529
                     /gene="hisC"
                     /locus_tag="PKK36_06605"
     CDS             5480..6529
                     /gene="hisC"
                     /locus_tag="PKK36_06605"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNR94264.1"
                     /translation="MNKYVRKNVNRMKGYTPGEQPSDPLIIKLNTNENPYAPSPAVGR
                     ALEDFDFTNLGLYPDPLATKLRGMIAAYHGCETCRVLAGNGSDELLALCVRAFVEGRG
                     KIGFFEPSYSLYPVLAEMHGLRAIAVPLGSDFEWAMPDEVDASLFFLANPNAPTGIMY
                     PMEQVRHFCERFPGVVVIDEAYADFAEANCMSLALELENVIVLRTFSKSFSLAGLRLG
                     YAVGAPELVGAMSKLKDSYNVDRIAQTLGCAAFADMDYMRSNCENIKNIRRHTAAELE
                     KRSFRVFPSQANFLWVEPSGIEAAVLFEKLRERNILVRYFPGPRTGRCLRISMGTRAQ
                     MDVLLGAVDRILQLK"
     gene            6544..7467
                     /locus_tag="PKK36_06610"
     CDS             6544..7467
                     /locus_tag="PKK36_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94265.1"
                     /translation="MLKIGRMILGLAVATPFVRAAEIGDQGRVCFQLNRGQIVQFADA
                     LSIEDIREDQWKPVGTTGCDEGAAAAMAGTDMLRFYRVRPGGESTYAFGVVNLHPGRN
                     WIGLWGDPEINSVRAVFGDRMPTGTLMRIMPPDSAAEIALLLAKDQAGAVRWMSGENP
                     ADETEIPAEVSAVEVELPPGCGPVAVPVILKVPAAPVEQILSYGKRTLGGFRCPGRPR
                     LDRTGLLAGGFTGGANPVESDQLWKYDRLNQRAPYAVWFCTRDNTWRLTVGGPDFPVV
                     PADAFRYDDAVMVMPRKALMGIRWRKGGSVK"
     gene            complement(7492..8475)
                     /locus_tag="PKK36_06615"
     CDS             complement(7492..8475)
                     /locus_tag="PKK36_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HNR94266.1"
                     /translation="MKAPKQKAAGWLTYIPELEVVDCTVRDGGLINAHHFGDDFVRAV
                     YHTCIDAGLDYMEFGYKASKKIFSKAEYGAWKFCDEEDIRRITDTRDRGTLKFCAMAD
                     AERTDYHTDILPKKDSVLDCIRVATYVHQLPTALDMVKDAHDKGYMTSLNLMAVSAAL
                     ELEVDNALEVMAASEVDVLYIVDSYGAFYTEQIEALMEKFKKVCKGTSKRIGIHTHNN
                     QQMAYSNTITAIINGATMLDATLCGLGRGAGNCPMELLLGFLKNPKFDLRPILQASQE
                     VLLPMRKTMDWGYSIPYAITGQLNEHPRDAIKMREGPEPDNYVRFFDEMNE"
     gene            8601..9665
                     /locus_tag="PKK36_06620"
     CDS             8601..9665
                     /locus_tag="PKK36_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR94267.1"
                     /translation="MSGTNRTKLMALLVNAAAFVVIVAGMRAASSIIVVLLLAVFISV
                     VITPLYFGMQRRRIPSWLALLLLITAMIVLTVVAVVVLGKSVQDFSSNLPRYQQSLQA
                     QLAGSVEWLRAKGYEIPDDAVARVMNTKTALTFVGNTLQAVSTLLSQAFLVLLIVIFM
                     MLEAAMLPAKIQSISAMTPEHRERLRMAVNTFRQYMSIKTFVSLITGGLVTLMLAVFK
                     IDYAVLLGVLAFILNYVPNVGSIIAAVPAIALALIQFGPGMSLLVAIGYLIINTLIGN
                     IWEPRLMGRRMGLSPMILVISLVFWGWVLGPVGMLLSVPLTMIFRLILESIEETRGLA
                     SLMAAAAPRQGPPAESASGD"
     gene            9658..10290
                     /locus_tag="PKK36_06625"
     CDS             9658..10290
                     /locus_tag="PKK36_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNR94268.1"
                     /translation="MIKLLAIFGMAFLVGFSGAVAPGPVFTMTISESLKRGFRAGPLI
                     VLGHAVLEMLLLAAIMLGLGVWLTRPEVTRFFALVGGIILLAMGGHMVATAGKATDEA
                     LHAEASAGDAVRGPVMAGVLLSLSNPYWIIWWATIGLGLITKSLEAGLLGLVFFYCGH
                     ITADLTWYSFVSYTVSAGRRLCPPRVYHVIFIVCGAGLVGLGLWFLKSLF"
     gene            complement(10302..10739)
                     /locus_tag="PKK36_06630"
     CDS             complement(10302..10739)
                     /locus_tag="PKK36_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNR94269.1"
                     /translation="MKRDIPVRFNDIIRTKEGYAVFVTDGIKNIAFFIDGFTGEAICM
                     AREGVRTMRPHTHMLIESIIAGLGAKVEKAVVHTLKEDIYFAHLYLSQPAGEGKHIVE
                     IDARPSDCIALALELDAPLFVKQDVWEASEDMTWVLEQHDKDE"
     gene            complement(10831..>12914)
                     /locus_tag="PKK36_06635"
     CDS             complement(10831..>12914)
                     /locus_tag="PKK36_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR94270.1"
                     /translation="LDPVIGRDSEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLA
                     QRIVAGDVPEGLKSKRVVSLDLGQMVAGAKYRGEFEDRFKAFLKEVTEASGQIILFID
                     ELHTLVGAGGAEGAMDASNMIKPALARGELRCVGATTLDEYRKHVEKDAALERRFQPV
                     MVEEPTVEATIAILRGLRERYEVHHGVRIQDAALVAAATLSDRYISDRFLPDKAIDLV
                     DEAASRLRMEIDSMPVEIDEIERRIMQLEIERSALRKEKDEGSKERLQKIEQELAELS
                     EKSSKLKLHWNSEKEMISEIRHLTQALDLLRNREEAAKRSNDLESAAEIMYDKIPKTE
                     KQIQSAQQKLAELQKKDRILKEEVDAEDIASVISSWTHIPVSRLLESEKHKLMEMEQR
                     LQQRVVGQQEAVEAVSNAVRRARSGLQDPNRPIGSFIFMGPTGVGKTELARALAEFLF
                     DDEQAILRVDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEHVRRKPWSVVLFDEIEK
                     AHPEVFNVLLQILDDGRLTDGQGRTVNFTNTIIIMTSNIGSAIIHEALVLEPDLKRGD
                     RAYQVMEQRVMEALRAQFRPEFLNRLDEIIIFHSLNRKHIREVVEIQLNRIRKHLAGR
                     SIELSLTDEAKNHLAETGYDSSFGARPLKRVLQREILDALASEIIEGRIPEGARVEAE
                     YKNGKIEFKIR"
BASE COUNT         2336 a         3656 c         3945 g         2977 t
ORIGIN      
        1 accccgcgcg cagtcaaggc cttcaggtcg ctgcaaagcg cgatcaccaa cgagatcgag
       61 gccgccctga atctgctgga ctggtccgga gtgctgattg atctggggca gcgcggcgat
      121 gcatcggtca tcctgtccca gcttttgacc gactatccgg cttcgcctca ggcggttccg
      181 ggcagcttgt ggttgagcag aattcacctg gaggaaggcc ggcttccaga ggcggagaag
      241 cttctgctgg atctgatggc gcgcgagaac ctggccgatg acagccgtgc agaggcctgt
      301 cgtgtactgg ctgcagttgc agaagcgcag acgaactata cccgggccgt ggagttcctt
      361 gaccgggctg tgaagctttc cgccgatccc gtggttctga accagagtcg tttgcgcaag
      421 gcgcgtctgc tgatccgcgc gggccggcgg gacgagggcg aagctctgct tcatgagtgg
      481 attggcgcca atacggctaa tccgcacgcg ccggaatccc tcttgacttg ggcggacgag
      541 ctttttgcct ccggagagta tgagaaagcg taccgtgaat atcagtatta tcaggaggtc
      601 ttttcggaca cgccgggccg tttccgggct cagatgggca agggctggtg ccttttcggt
      661 cttaagcggc atgccgaggc cgtcagggag tttgaacggg ccctctcgct cgccgaaggc
      721 gatgcggatc ggaaacaggc ccttttcaag attgcggatg ctctttatgc agacggcgaa
      781 tacacggcag cgcgggcgaa atatcttgaa gtaacgaagc tttttcctgc cgcgcaggag
      841 gctccgcagg ccctttatca ggcggcggag tgttatgccg aagcgggtca gtatgacgcg
      901 gccgaaaagg agtttcacgc cattgaggat gcttatccgg aaagcggctt tgcgcgtcag
      961 gcatcgctgc gaattgcggc gatgaaggaa ctgatcgggg aatgggagca ggcggtatcg
     1021 cagtacaacc ggattctccg cgagttcccg ggaagcctgc acgcgggacg ggccagacat
     1081 cgccggggca tggtgcgcta tcgccttggg agtttcccgg gcgcccttgc ggatttcgaa
     1141 aaagtggtgc aggagtttcc cggccacgat ttctccgagc aggcgtttta tatgcgcgga
     1201 tggacacatt atctgatggg ccagtccgac aaggcccagg atatctgtcg tgagtttgtg
     1261 cagaaattcc ccgattccgc atgggcgccg gacgtgcttt tctggctggc cgaatatcag
     1321 ttcaacaacg ggttccatca gaaagccgaa gccggattta ccggagtggc gcttcgctat
     1381 ccttcatccg aactggcaga cgacgcgatt ttctgggccg gtcagtctgc agtgctgcag
     1441 cgtgaatatc tgcgggccat cgagcatttc agcaaactgg ttcaaaatta tccggagagc
     1501 cctcgtctgg tgcaggcccg ttttgcgcag ggcgacgcgc tcagcgaact cgggcagttc
     1561 gcagccgcga tcctggctta tgaagaagtt attctcaagg cgcagggctc atacaccgcg
     1621 gcgctggccc ggggaaggat cggcgactgc cagttcactc tgggcgcagg cgatcccgcg
     1681 cgttatgcgg aagcgatcaa atcctatgcc gccctgacgg gccgagccga tgtcccggtt
     1741 gaaatcagcc ttcaggccga atataagctt ggccgttgtt tggacaagac cggggatgct
     1801 gcgggagcgc tggagcgata cctgaatgtg atctatcgct atgtggccca gaaaaaccgc
     1861 gccgtgggaa gcgaggcctg gtttacacgc gcagcgttcg gtgcggcggc cattcgggag
     1921 caggagcagc agtggtcgga agccctccgt atttatgaac gcataatcga ggaacaggtg
     1981 ccggcggcgg aggaggcgcg catgcgcatc cgcaagctgc gcacagaacg atgggtgacg
     2041 cccggaacgg attgaaggag aaacagcaca tgcaggaaat gatcaatgca ggcggaccga
     2101 tggtctgggt gattctcgcg gccggcgttc tcgccgccac ggtctttgtg gagcgcctct
     2161 ttcatttgca tcgcgcccag atcaagtcgg acgattttct gaaaggcatc tacaacatcc
     2221 tgaaccgcgg caacatcgtg gaggctgtca gcatctgcga ggatactccg ggcccggttg
     2281 cggatgtcgt gcgggccgcc atcctcaatc atgatgcagc cgtgcctgag atggaaaagg
     2341 cggtgcagga ggccggactg ctggagattc cccggctgga acggaatatc agcacgctgg
     2401 ccaccgtggc acagcttgcg ccgctgctgg gtttgctggg gacggtgctg ggcctgatgc
     2461 agaatctgct ggttatacag cagaaggctc cccttgtgca gcccggcgat cttgcgggcg
     2521 gactctggca ggccctcatt tccacggcag cgggattgac gctcgccatc ccggcccatg
     2581 ccgcctacaa cctgctggtc agccgggtgg aatccattct gctcgacatg gagagggctt
     2641 ctgtcgaaat catcgcctat ttgagcaaga aaggcgcagg gccgcggtga tgcggagcgg
     2701 tgtatatctc aggctttcca agcttcaccg cctgtatgcg gcaaggaacc ggatacggcg
     2761 cggtccgccg ggactcattc cgctgctgga cctggttttg atccttttcg ttttctacat
     2821 ggcacacgcc ccgtttgtgc ttcagcccgg cattacgatg acccttccgt cgtctccttt
     2881 ccgcgacggg gtgcagttcg gccgccttct ggtcaccgtg acccgggacg gggtcatatt
     2941 cttcaacgat gaccgcgttt cactgcagga actgcccgcc agattccgga atgcggccga
     3001 tgacgaaagt ctgattatcc aggcagacgg ttttgtgtcc catcacatgc tggttcaaat
     3061 ctataacatg gcgtctgacg caggtatccg cgaggtcgtt ttggccgctc gcgagccggg
     3121 cgtgaaggga ggtgtgccgc gatgaaaatg tccgcggaac gttgcgccgc agaccggcgc
     3181 attcgggcca ttgcggctgc aggctcatgc ctgtgggttg gattgtttgc ggtcctcttt
     3241 cgtccggcgg ctcccctggc gtacgccgcg gtttctcctt ccgcccggct tctctattat
     3301 ccgatgctgg atgaaggcgg gtccggcttt gtgtcaccgg gcctctggtc cccccggctg
     3361 ttttcgtttc cctcatctct gggtttcagc gcttttctgg agacgcgccc gaatgatctt
     3421 cggccgcgcc tggaaccgct tgaaacagaa agcgagtttc tggcctattc tgcgccggaa
     3481 acagcccgga tccccgaggc cggcggtttg tccgcgcagg tgcgcgcgtt ttttgcctca
     3541 cagccttctc cctaccggtc tttgccgcaa gcgccgtctg gatccgtgtt tgatgtccgc
     3601 ttccttgaag ggctgtcaga agaggatttc atggacatat cgctccccgc tgcgctgtcc
     3661 tcaggggaag cgggctggca ggcgcagctg gaaatcgaag tacaacccga cggtacagtc
     3721 tcgcacgtgt ttctgatgaa cgaatcgcag cttcgcggaa accgcaacct gctggtgcag
     3781 gcggcgcgga agtggagaac cgttccttcc gataaagtgc gccggggctg tgtgactgtg
     3841 cggagcgtgc cgccgtctcc gggggtgcgg ccatgattca ggtcagctgg ccggtaatct
     3901 tctatgtgcc cctggctttg aatttactgg ttgtttcgct gatcggcttg ttctacttta
     3961 ttcgccggta cgaaccgcat gttcgatatg ccaatgagcg catctaccgg tgcgctgcat
     4021 gtggacatgt ctatgtggat tcgaggaaaa tgccgctgct gcgctgtccg aggtgtgaca
     4081 gcctgaatga ggccgtgcgc acacactagg tggcgccagc ttccgggaga gagatttttc
     4141 gatggttaag aacgcaaaac tggatatcaa atcggtccgc tggaccgtga cttcgcagcc
     4201 ggcagaggtg gaggagttcc ttcagcgccc ggcctttgat ccggccgccg agaaagcggc
     4261 cgccaggatg ctggccgcca tccgcagccg cggcgacgag gccgtgctgg aatttattga
     4321 aaagcacgat ggggtaaagc tgtcggccgg agctctctgt gtcactccgg aagagcgaga
     4381 aggggcgcgc gcagccgtgg acgacgattt caagcgtgcg gcccgagaag cgcgcaggcg
     4441 catcagtcga ttcgcggagg ccggcattcg caaggactgg agcatcacca gccccaaggg
     4501 cggcatgatt ggcgagcgtt ttgtcccgct cgacagagtg ggcgtctata ttcccggagg
     4561 agctgctccg ctggcttcca cggccatgat gaccatcgtg ctggcccgcg cggcaggggt
     4621 cccggaggtt gtggcctgca cacctgcggg ccgggacggg accatcaatc cggcgctgct
     4681 ctatgccatt gattttgccg gggcaacaga aatttaccgc atcggaggca ttcaggccat
     4741 cggcgccatg gcctatggga cctccacagt ccgtaaggtg cagaagatcg tcggtccggg
     4801 agggccgtac gtaaccgccg ccaagcggca ggtttatggc gaggtttcac tggacatggt
     4861 cgccggcccc agtgaaatcg ccgtgctggc cgatgattcc atcagtccct cgtttgtggc
     4921 cgccgacctg ctttcgcagg ctgaacacgg gagcggcgct gaaaaggttc tgctggtaac
     4981 gccttcagag cgtttggctg acggtgtggc tcgcgaactt cttgtgcaga gtgaaaccct
     5041 gccgcgccgg gattttgtcc gcaaggtgct gcagcacggc atgctgattg ttgttacgga
     5101 caatctggat gcggggatgg aagtatgcaa ccggtttgcg ccggagcatt ttgaacttct
     5161 ggtgcaggag ccgcggcgct ggatacagaa ggtgcgcgcc gccggcgctg tttttgccgg
     5221 tccgtggact ccggaaagcg cgggcgactt tgcgatcgga ccaagccatg tgctgccaac
     5281 cggcggaacg gcagcctttt tttcgggtct gacggtggat gattttcagc gtcgcatgag
     5341 tgtgatgtct cttacccggg cggatttgat tgaactcctg cccgtgatcg aggctttcgg
     5401 gcgcgtagag gggcttgaag cgcatgcccg ttccgcccgc atccgttttg aatcgtgaac
     5461 caagtgcagg ggcagcatca tgaacaagta tgttcgcaag aatgttaatc gtatgaaggg
     5521 ttacacgccc ggcgagcagc cctcggatcc gctcatcatc aagctgaata ccaatgagaa
     5581 tccgtatgcc ccttcgccgg ccgtgggccg ggcgcttgag gattttgatt ttacaaatct
     5641 cggtctgtat ccggacccgc tggccacaaa gttgcgcgga atgattgcgg catatcacgg
     5701 atgcgaaacc tgccgggtgc tggcgggaaa cggatcggac gagctgcttg cgctgtgtgt
     5761 gcgggctttc gtcgagggaa ggggcaaaat cggatttttt gaaccctcct attcacttta
     5821 tcccgtcctg gcagaaatgc acggcctgcg cgccatcgcc gttccgctgg gttccgattt
     5881 tgaatgggcc atgccggatg aggtggatgc ctcgctgttc ttccttgcga atcccaatgc
     5941 gcccaccggc attatgtatc cgatggaaca ggtccgccat ttctgcgaac gtttcccggg
     6001 agtggtggtg atcgacgagg cttacgcgga ttttgccgaa gccaactgca tgagtcttgc
     6061 gctggaactg gagaatgtca tagtccttcg gactttttcc aagtcgtttt cccttgcggg
     6121 gctccgtctg ggctatgccg tcggggctcc ggagctcgtc ggggcgatgt ctaagctcaa
     6181 agactcctac aacgtggatc ggattgctca gaccctgggt tgcgccgctt ttgcagatat
     6241 ggactacatg cggtcaaatt gtgagaacat aaaaaacatc cgcaggcata cagctgctga
     6301 actggagaag cgctctttcc gggtcttccc gtcgcaagcc aatttcctgt gggtggaacc
     6361 ttcaggcatt gaagccgcgg ttttgtttga gaaactgcgc gagcggaaca ttctggtgcg
     6421 ctatttcccg ggtccgcgca ccgggcgatg cctgcgcatt tccatgggca ctcgcgcgca
     6481 gatggatgtg cttcttggtg ctgtggatcg cattctgcag ctgaaataac tggagatcac
     6541 gccatgttga aaatcggcag aatgatcctt ggtcttgcgg ttgcaacgcc gttcgtccgc
     6601 gccgcagaga tcggggatca ggggcgggtg tgcttccagt tgaaccgtgg acaaatcgtt
     6661 cagtttgccg atgccctttc gattgaagac atccgggaag atcagtggaa gcctgtcgga
     6721 acaaccggat gtgatgaagg cgcggcggca gccatggcgg gaaccgatat gctgcgcttc
     6781 taccgggtcc gtccgggcgg ggagagcaca tacgcctttg gggttgtgaa tctgcatccg
     6841 ggacgaaact ggattggcct gtggggtgat ccggaaatca attctgtgcg cgctgttttc
     6901 ggcgaccgca tgccgaccgg aacactcatg cggatcatgc cgccggattc ggccgctgaa
     6961 attgctcttc ttcttgcgaa agatcaggca ggagcggtgc gctggatgtc gggtgaaaac
     7021 cccgccgatg agactgaaat tcccgcggag gtctccgctg tcgaagtaga acttcctccg
     7081 ggatgcgggc ctgtcgctgt gccggtgatc ctgaaagtgc cggccgctcc tgtggagcag
     7141 attctgtcct acgggaaaag aacgctgggc ggattccgct gtccgggccg cccccgtctt
     7201 gacaggacgg gcctgctggc gggcggcttt acgggcggcg caaatccggt ggaatcagat
     7261 cagctctgga agtacgaccg tttgaatcag cgcgcgccct atgcagtctg gttttgcacc
     7321 cgggacaaca catggcggct gactgttggc ggtccggatt ttcctgtcgt gcccgccgat
     7381 gccttccgtt atgacgacgc ggtcatggtg atgccccgta aagcgctcat gggcattcgc
     7441 tggcgaaaag gcggatccgt aaaataacgg ccggttccgt gactgcggag cttattcatt
     7501 catttcgtcg aagaatctga catagttgtc cggttcaggt ccttcgcgca ttttaatggc
     7561 atcgcgcggg tgctcgttga gctgtccggt gattgcgtag gggatgctgt agccccagtc
     7621 catggtcttg cgcattggca gcagcacttc ctgcgatgcc tgcaggatgg ggcgcaggtc
     7681 aaatttcgga ttcttcagga aacccagcag cagttccatc ggacagttgc cggctccgcg
     7741 tcccagtccg cacagtgtgg cgtccagcat ggtcgctccg ttgatgatgg cggtgatcgt
     7801 gttggagtag gccatctgct ggttgttgtg ggtatggatt ccgatgcgct tggaggtgcc
     7861 tttgcagact ttcttaaact tctccatcag agcctcaatc tgctcggtat agaaggcccc
     7921 gtaactgtcc acgatgtaaa gaacgtccac ttcgctggcg gccatcactt cgagcgcgtt
     7981 gtccacctcg agctccaggg ctgccgagac cgccatcagg ttcaaagatg tcatatagcc
     8041 cttgtcatgg gcatccttga ccatgtccag cgcggtgggc agctggtgca cataggtggc
     8101 cacccggatg cagtcgagca cactgtcctt cttcgggagg atgtccgtat ggtagtccgt
     8161 gcgctccgca tctgccatgg cgcagaattt gagggtgccc cggtcacgtg tgtcggtgat
     8221 ccgacggatg tcttcttcat cgcagaactt ccatgcgccg tactccgcct tgctgaatat
     8281 cttcttggag gctttgtatc cgaattccat gtagtccagc ccggcatcaa tgcaggtgtg
     8341 gtagaccgcg cgcacgaaat catcaccaaa gtggtgtgcg ttgatcagac ccccgtcacg
     8401 cacggtgcag tccaccactt cgagttccgg tatatatgtc agccatccgg cggctttctg
     8461 ttttggagct ttcatagtgg cgctaatata tatggatcgg ccggattaac aagctcggcc
     8521 tgacagaata ttcttttcgg aattgggatt gacgccgcct tggcttgatt tccccgtctc
     8581 tctctgcgag gatggtccgc atgagtggaa caaaccggac caaactgatg gcgctgctgg
     8641 tcaatgcggc ggcgtttgtg gttattgtag cgggcatgcg cgcggcctcc tcgataatcg
     8701 tggtgctgct gctggcggtt ttcatcagcg tagtgatcac ccctctctat ttcggcatgc
     8761 agcgccgaag aattccctca tggctcgctc ttctcctgct cattacggcc atgattgtcc
     8821 tgaccgttgt cgcagttgtg gtgctgggca agtcggttca ggatttctca agcaatcttc
     8881 caagatatca gcagagcctg caggcccagc tggccggaag cgttgaatgg ctgcgggcaa
     8941 agggctatga aattcctgac gatgcggtcg cccgcgtaat gaatacgaaa acagccctga
     9001 cctttgtcgg gaacacgctt caggccgtca gtacgcttct cagccaggct tttctcgtgc
     9061 tgctgattgt cattttcatg atgctggaag cggccatgct gccggccaag attcagtcga
     9121 tttccgccat gaccccggaa catcgggagc gcctgcgcat ggcggtaaac accttccgcc
     9181 agtatatgag catcaagacc tttgtcagcc tgatcacggg cgggcttgtt acgctgatgc
     9241 tggcggtctt caagattgac tatgccgttt tgctgggtgt cctggccttt attctgaatt
     9301 atgtgccgaa cgtgggttcc attatagcgg ctgtgcccgc catcgcgctg gcgctgatcc
     9361 agttcggccc gggcatgagc ctgctcgtgg ccatcggcta tctgatcatc aacaccctga
     9421 tcggcaatat ctgggagccc cgcctgatgg gcagacgaat ggggctctcg ccgatgattc
     9481 tcgtcatctc gctggtgttt tggggctggg ttctggggcc ggtcggcatg ctgttgtctg
     9541 ttccgctgac aatgattttc cgtctgatcc tcgaaagcat agaggagacg cgtggtctgg
     9601 cctctctcat ggcggccgct gcacccaggc aggggccgcc ggcggagtct gcatccggtg
     9661 attaagcttc tggccatatt cggcatggcc tttctggtcg gattctcggg agcagtcgct
     9721 ccgggtccgg tgttcaccat gaccatcagt gaatctctca aacgcgggtt cagggcgggt
     9781 ccgctgattg tgctggggca tgcggttctg gaaatgctgc tgctggcggc cattatgctg
     9841 gggctcggcg tctggctgac caggcctgaa gtcacccgtt tctttgcgct ggttggcgga
     9901 atcatcctcc tggcgatggg ggggcacatg gtggccacgg cagggaaggc gaccgacgag
     9961 gctcttcacg cggaagcgag tgcgggagat gccgtccgcg gccctgtgat ggcgggtgtg
    10021 cttctcagcc tgtccaatcc gtactggatc atctggtggg cgaccatcgg gctgggtctg
    10081 atcaccaagt ctcttgaggc cggcctgctt ggactggtct tcttctattg cgggcacatc
    10141 acggccgatc tgacctggta cagctttgtt tcctatacgg tatctgccgg ccggcggctc
    10201 tgccctcccc gggtatacca tgtgatattc atcgtctgcg gagccggcct ggtcggcctc
    10261 ggcctctggt ttctgaagag cttgttctga gcagcggacg gctattcgtc cttgtcgtgc
    10321 tgctccagca cccacgtcat gtcttcggat gcctcccaaa catcctgttt gacaaaaagc
    10381 ggggcatcca gttccaaagc cagcgcgatg cagtcactgg gccgcgcatc aatttcgaca
    10441 atgtgctttc cctcgcccgc aggctgagaa agataaagat gcgcaaaata gatatcctct
    10501 ttcagcgtgt ggacaacggc cttttccact ttggctccga ggccggcaat gatgctctcg
    10561 ataagcatat gcgtgtgagg gcgcatggtc cggactcctt cgcgcgccat gcagatggcc
    10621 tcaccggtga aaccgtctat gaagaaagcg atgttcttga tcccgtccgt gacaaatacg
    10681 gcatatccct cctttgtgcg gatgatgtcg ttgaagcgga cagggatatc tcttttcatg
    10741 atggtccgtt cacatgtcgc gggagatgta ccacacggcc cagaatatga atatgacgag
    10801 tgcagcagac agaaaagtgt tcattggcgc ctatctgatt ttaaattcaa tcttcccgtt
    10861 tttatattct gcttcgacgc gggccccttc cggaatgcgt ccttcgatga tctcactggc
    10921 gagggcatcg agaatttccc tctgaaggac tctcttgagc ggccgcgcgc caaaagagga
    10981 gtcatagccg gtttcggcca gatggttctt tgcttcatcc gtcaggctca actcgatgct
    11041 gcgcccggcc agatgttttc tgatccggtt gagctgaatt tcaacgactt ccctgatgtg
    11101 cttccggttc aggctgtgga agatgatgat ttcatccaga cggttgagga actcgggacg
    11161 gaactgcgcc cgcagagctt ccatcacgcg ctgctccatg acctgataag ccctgtctcc
    11221 gcgcttcaga tccggttcca gaaccagagc ttcgtgaatg atggcactgc cgatgttgga
    11281 cgtcatgata atgatggtgt ttgtgaagtt tactgtgcgg ccctgtccgt cggtcagccg
    11341 gccgtcatcc aggatctgga gcaggacgtt gaacacttca ggatgggctt tttctatttc
    11401 atcaaacagc accaccgacc atggtttgcg gcggacatgc tcggtgaggt aaccgccctc
    11461 ctcatagccg acatagcccg gcggcgcgcc gatcaggcgc gccacactgt gtttctccat
    11521 gaattcagac atgtccaccc gcagaatggc ctgttcgtcg tcgaacagga attcggccag
    11581 cgcgcgcgcg agctcagttt ttccgacgcc ggtcggcccc atgaaaatga atgatccgat
    11641 gggccggttg ggatcctgca ggccggaacg agcccggcgc accgcatttg aaaccgcttc
    11701 cacggcctcc tgctggccga ccacccgctg ctgcagccgc tgctccattt ccatcagctt
    11761 gtgtttctcg ctctccagca gccgtgagac cggaatatga gtccagcttg agatcacgga
    11821 agcgatatct tccgcgtcca cttcttcctt caaaatccga tccttcttct gcagttcggc
    11881 cagtttctgc tgcgcggact ggatttgctt ttcggtcttc ggaatcttgt cgtacatgat
    11941 ttccgcggcg gactcgaggt cgttgctgcg cttggcggcc tcctccctgt tccgcaggag
    12001 atccagcgcc tgggtcaggt gccggatttc ggatatcatc tctttttcgc tgttccagtg
    12061 cagcttaagc tttgagctct tctctgaaag ctccgcaagc tcctgctcga tcttctgcag
    12121 acgctccttt gaaccctcat ccttctcttt gcggagggcg gagcgctcga tttccagctg
    12181 cattatccgg cgttcgattt cgtcaatttc aacaggcatg ctgtctattt ccatccgcag
    12241 ccggctggcg gcctcgtcca caaggtcaat ggccttgtcc ggcaggaacc ggtcgctgat
    12301 gtagcggtcg gaaagtgtcg cggcggccac cagtgcggcg tcctggatgc ggaccccgtg
    12361 atggacctcg tagcgttcac gcagtccgcg caggatcgcg atggtggcct cgaccgtcgg
    12421 ctcctccacc atcaccggct ggaaacgccg ttcaagcgcg gcatccttct caacatgctt
    12481 gcgatactcg tccagcgtgg tggcgcccac gcagcgcagt tctccgcgcg caagcgcagg
    12541 cttgatcata ttcgacgcat ccatcgcgcc ctcggcccct cccgcgccca ccagtgtgtg
    12601 aagctcgtca atgaagagaa tgatctggcc gctggcttcg gtcacctctt tcagaaaggc
    12661 cttgaagcgg tcttcgaatt ctccccggta tttggccccc gccaccatct gtccaagatc
    12721 gagcgagacc acgcgcttgc tcttgagccc ttccgggaca tccccggcca caatacgctg
    12781 tgcgagcccc tccgcaatcg cggtcttccc aaccccgggc tctccgatca gcacgggatt
    12841 gttcttggtc cggcgagaaa gcacctgaat gacgcgccgg atttcgctgt cccgcccgat
    12901 gacgggatcc agtt
//
LOCUS       DAOQWR010000066        12885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5361_length_12885_cov_5.760405, whole genome shotgun sequence.
ACCESSION   DAOQWR010000066 DAOQWR010000000
VERSION     DAOQWR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_12885_cov_5.760405"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKK36_06640"
     CDS             <1..246
                     /locus_tag="PKK36_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR94271.1"
                     /translation="DELSAGEARRTLDEMARLDRVLGFLTAAEDEIPAEVTALVEARK
                     QARAEKNWAASDRIRDELAALGWTVKDTPEGPKLRKL"
     gene            243..902
                     /gene="tmk"
                     /locus_tag="PKK36_06645"
     CDS             243..902
                     /gene="tmk"
                     /locus_tag="PKK36_06645"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNR94272.1"
                     /translation="MKKVMRGKFITFEGPEGGGKTTQARRLVETLEGLGREVIYTREP
                     GGTPTGEAIRGILQYNTAGEPVCPETEVLLFAASRAQLVRHVILPALERGCWVVSDRF
                     ADSTTAYQGYGRGFPVESMLTINAFAISEAVPDITFLLDIDIGLGFERLAERHRQTNT
                     AKDRIEQEERAFHERVRQGYLQLAERWPERFRRLDAARSPDEVAAEVWSILEGFTNIT
                     G"
     gene            874..1848
                     /locus_tag="PKK36_06650"
     CDS             874..1848
                     /locus_tag="PKK36_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94273.1"
                     /translation="MKDSQTSQADRALEYFRAAAESDRLSHAWLVACTDILDGSGFAE
                     KALAFLFCGGLIKPCGDCPACRQVAAHKHADIQWVEPVLKSRRISIEQMLEVNSRLAR
                     SSYSGGWKAAVLLHADRMTDEAANAFLKTLEEPSGRTILFLVTDSIEDCLPTILSRCH
                     RVALQRGERPGTAVWMDDLLAFMRTLPSGGCAEGAAHAAELAALIDRERKRIEQEMED
                     RPADDDSKKSKEIFEARVSAALRRERSEMLRQLALWQRDILFASQGLGDEAFHFRGDA
                     ESIRRQAQDITWTSARARVQAVEDMSRRLEQNLNPITVLELGMAALAG"
     gene            1915..2355
                     /gene="aroQ"
                     /locus_tag="PKK36_06655"
     CDS             1915..2355
                     /gene="aroQ"
                     /locus_tag="PKK36_06655"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNR94274.1"
                     /translation="MKILVLNGPNLNLLGRREPDIYGRLTLADIEESMRGHARKCGVE
                     VDFFQSNDEGALVSRIGEAAGLYDGLIFNPAAYTHTSVALRDALMAVKLPCVEVHLSN
                     PQNREDFRQANLTLGACIGQVSGFRDRSYILAMDGLIHYLKNKS"
     gene            2391..2843
                     /gene="accB"
                     /locus_tag="PKK36_06660"
     CDS             2391..2843
                     /gene="accB"
                     /locus_tag="PKK36_06660"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNR94275.1"
                     /translation="MQLNEIKKILSLMKENDLAEFEMQDGDSRIHIKRGAGGVPVMMQ
                     PPPVMMPAAAPAPAAAPAAAPAPAPAAGPVIKSPIVGTFYRSASPDADAFVSVGSEVN
                     PDTVVCIIEAMKVMNEIKADVKGVIRKVLVDNATPVQYGQALFEVEPR"
     gene            2849..4213
                     /gene="accC"
                     /locus_tag="PKK36_06665"
     CDS             2849..4213
                     /gene="accC"
                     /locus_tag="PKK36_06665"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNR94276.1"
                     /translation="MFEGVLVANRGEIAVRIIRACRELGIRSVAVYSEADEHSLHVQL
                     ADEAICIGPAAASESYLKISSIISAAAVANVDAIHPGYGFLAENAAFAEICQNCNITF
                     IGPDPEHIRKMGDKAVARETMMEAGVPVTPGSEGILKNKDEAIKLARSIGYPVLVKAV
                     AGGGGKGMRVAHNDVALAQAFSMASAEAERAFGNASLYLEKFIESARHVEVQIMGDKH
                     GQIVHLGERDCSIQRRHQKLIEETPCPVLTDAIRKKLHATAVKAAAAVGYHNAGTIEF
                     LYDELAKEFYFMEMNTRIQVEHSVTEEVTGLDLVKEQINVAAGQKLSFTQEEAEPQGH
                     AVEMRVNAEDPYNNFSPCPGLVEWVHLPAGRGIRVDSHVYSGYTISPFYDSMIAKVIA
                     HGKTRQQALARMQGALEEFMIKGVETTAPLGRALMTDEHFRKGRYNTHYLETFMNESF
                     LLKS"
     gene            4216..4791
                     /locus_tag="PKK36_06670"
     CDS             4216..4791
                     /locus_tag="PKK36_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94277.1"
                     /translation="MKTLKILLALVRGLLMALGIGCLVYAPFSGGCMGGGLTFFREQY
                     VALIFVLLLAGLLLLPGRIRLRGKKEMEFIPLNGPGGDIRVSVRVAKNTVQECAAKFD
                     AIRSLEPELSWSSEGLSVVMRLQMQRGRPLTTVCMDLQEAVREIFAGELGIEQLREVR
                     ADIIELSAAAKAAETLPVPPPEVESKPEQGA"
     gene            4793..5356
                     /locus_tag="PKK36_06675"
     CDS             4793..5356
                     /locus_tag="PKK36_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012980017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNR94278.1"
                     /translation="MDWNRMAEEYLGVADELFNRLKGEIADLAELIAARLKAGGKVMI
                     CGNGGSAADAQHFAAELVNRFLMERRPYAAIALSTDTSILTAVGNDYGYEFVFEKQVQ
                     ALGKKGDILIAISTSGNASNVCRAVDAAKALGVTTLALTGGRGGKLAGLADHVLSVAS
                     TSCVPRIQEGHQFIMHAVCERVEEILK"
     gene            5376..6296
                     /locus_tag="PKK36_06680"
     CDS             5376..6296
                     /locus_tag="PKK36_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR94279.1"
                     /translation="MPERTVDIVIVGSVGIDTVETPYARRESILGGSASYACMAASYF
                     APTGMVGVVGADFPEAYRKLYADAGINLDGLRTVPGDTFRWEGKYEANMDNRSTLAIA
                     LNVFEDFKPELPAGYVNAPYIFLGNISPQLQMHVLDQVKSPEFVLADTMDLWIQTAPD
                     DLKKLISRVDMLTLNESEARALTGGRYSLIRAGRDLLELGPKYVMIKKGEHGSLLITR
                     DNLFVLPAFPLERVVDPTGAGDTFAGAFLGALARSGGISEKNLRRAMRCGAVVASIGI
                     EAFSLDALRHLTLAEIEKRVSQFRAYTAID"
     gene            6653..8374
                     /gene="ilvB"
                     /locus_tag="PKK36_06685"
     CDS             6653..8374
                     /gene="ilvB"
                     /locus_tag="PKK36_06685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNR94280.1"
                     /translation="MTKTTMDGGQAVIKCLENEGVEFLFGISGGSAIPIFDAIVTTST
                     SIKMILTRHEQGAAHMADGYARATGKPGVVLVTSGPGATNTLTGIMTAHMDSVPMIVL
                     TGQSIRPMIGKDAFQEADIFGMCMPVVKHSYLIKDTNDIPRIMREAFHICNTGRPGPV
                     LIDLPKDVTAGPCTADLAAEMDLPGYKLPDPPDKETVLEIAEALSKSERPVMMLGHGA
                     LIAGAAESAKILAEKMRIPVMTTFLGKGAFPETHRLSLGWPGMHGTAYANKALCECDL
                     LINIGGRFDDRILGKPALFCKDAVKIHVDIDPAEIGKMIRPDIHCVSDAKDFIDELTK
                     HVGHLNTGEWLAKLDGYKKKYPLKYRKGGGLKMQHVIEELYKITGGKAVVATDVGQHQ
                     MWAGQFYKTDYPNNWLSSGGAGTMGFGVPAAVGAQFGRPNELVVCITGDGGFQMCNQE
                     MTTAVVHKLPVKFIVLNNHYLGMVRQWQEMFYDDRKSGVDLEGNPDFVKLAEAYGMKG
                     FNLKRAGDIRKILKKAIEWNDGPCLINAEVEKTDNVFPMVPAGAALEDMLIEPPRHKL
                     EKPTGST"
     gene            8395..8910
                     /gene="ilvN"
                     /locus_tag="PKK36_06690"
     CDS             8395..8910
                     /gene="ilvN"
                     /locus_tag="PKK36_06690"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNR94281.1"
                     /translation="MANTELNNSIGVHTLSAYVANKPGVLARVAQVFSRRGFNIDSLV
                     VSSSIDGDFSRMTITATGNPDMLDQVIRQVNKLVDVIHCFDHTGQDVVVRELALIKIG
                     VTAEERTEALQVAEHFAAKTLDLTENSMIIMCTGRSSKLDALTGLLKKYKIIELVRTG
                     KVVMARGDDPT"
     gene            9193..10404
                     /locus_tag="PKK36_06695"
     CDS             9193..10404
                     /locus_tag="PKK36_06695"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94282.1"
                     /translation="MKKYVHRSLETVLKNLLKQFPAVAVTGPRQSGKSTLLLKTLPEA
                     RYVTLDDPVALQQALNDPELVLGNGDKPVVLDEIQYAPSLLPYIKMRIDRNRGAKGRF
                     ILTGSQQFALIKNLGESLAGRIGLLELLPFSVEEASRAGASSGSLARFERACLRGMFP
                     EPTMNSGMDVPRWYASYVQTYLERDIRTLYDIGNLREFERFLQLLAARCSQELHLTVL
                     ATDTGVAVNTIKKWISILEACRIIYLLPPYYRNLGKRIVKAPKVYFLDIGMVCYLTGL
                     RDRHHVINGPLAGALFENFCVQEALKAMLSKGVPPRLYFLKTHNGLEVDLLVEGINGR
                     LCPFEFKFSSTPKPAMCRSLERFGELFKDLNPEPGKLVSLSEKTAPLTRSIMHASLDV
                     FLSEIRNLAQV"
     gene            complement(10430..10567)
                     /locus_tag="PKK36_06700"
     CDS             complement(10430..10567)
                     /locus_tag="PKK36_06700"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNR94283.1"
                     /translation="MLEAGNVSANGNYITYNISVRVDSREIMDKIDTEVRSIEGVKLV
                     L"
     gene            10626..12089
                     /locus_tag="PKK36_06705"
     CDS             10626..12089
                     /locus_tag="PKK36_06705"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNR94284.1"
                     /translation="MSLFWSIGCGGRIRQRRRHRFCIGRMCSPVTFLIAACVFSIMVG
                     LLDLRAEDLAAPATLQFTSPTPASQLSTIGTFYSAQHPDWPPFPANVHDLPAWALGNG
                     CFLLADADFDYEAFAESMEAVNYTSGKYPMISRDYSSDDLWITINAVVNGRTDFTADL
                     TIHTPETSTCASWDLYYARALDWQTDWKFLMRCPTNIIGQTNVYARYMPDEIAFFMAR
                     ETTTNCDLSVTTNVTPQQMAEMLVPPWVTVTNVNYTGAAVARGIFANGNCCGLPIAGG
                     VILSSGYITDAAGPNDVDNKGDTVGGPPDADLNLLVGGSDSKDAAILEFDVISTNACL
                     LKFRYVYASEEYPEYSGSMYCDPFAIFVTTNRVGANWINTISNNIALVPVDAHIPVTV
                     YTVNGGYEHAYEGVIPTNAPYYVDNHDPYNQAVSPYLIGEPVFNIQYDGMTVLLAAQT
                     FVDACTTSHIKIAISDYTDSYYDSAVFLDEWSHDNSQ"
     gene            12104..12760
                     /locus_tag="PKK36_06710"
     CDS             12104..12760
                     /locus_tag="PKK36_06710"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HNR94285.1"
                     /translation="MQKIIYTLIASVCISWALSKPACGDLWVSNLDQFPIGSAAVGSD
                     FWIAQSFYIDISDSNQYALDAVQLLMNPATGSPNGFAVSIFSAPSDGLPQDYLGSLAG
                     SATPVTGGLYTYTASGITLSAGIYSVLVTSSTSTNQGAFNWSASDRSTQNGTWNIASV
                     YYTSSDGLNWQENGRADVFQMAIHATIVPEPATVWLLGAGLFSLSLCCRRKPRFTAPS
                     "
BASE COUNT         2735 a         3630 c         3880 g         2640 t
ORIGIN      
        1 gatgaactgt ccgccgggga agcccgtcgg acactggacg aaatggcccg tctggaccgg
       61 gtgcttggct ttctcacggc tgccgaggat gaaattcccg cagaagtgac ggcgctggtt
      121 gaggcgcgga agcaggcccg cgcggaaaag aactgggcgg cctccgaccg catacgcgat
      181 gagctggcgg cgctgggctg gaccgtgaaa gacacgcccg aagggccgaa actgaggaaa
      241 ttatgaagaa agtcatgcgc ggaaaattca tcacatttga agggcccgag ggcggaggga
      301 aaacaacgca ggcccggcgg ctggtcgaga cgctcgaagg gcttgggcgc gaagtgatct
      361 acacccgcga gcccggcgga acgccgaccg gagaggcgat ccggggcatc cttcagtaca
      421 acaccgccgg tgagcccgtg tgtccggaga cggaggtcct gctgtttgcg gccagccgcg
      481 ctcagctggt gcggcacgtg atcctgcccg cgctggagcg cggatgctgg gtggtctcgg
      541 accggtttgc ggattcaacg acggcctatc agggctacgg ccgcggattt ccggtggaga
      601 gcatgctgac catcaacgct ttcgcgatca gcgaagccgt gccggatatc acgttcctgc
      661 tggacataga cattgggctc ggatttgaac gtctggcgga gcgtcaccgg cagacgaaca
      721 cggccaagga ccgcatcgag caggaggagc gggcctttca cgagcgggtg cggcagggat
      781 atctgcagct cgcggaacgc tggccggagc gcttccggcg gctggatgcc gcccgcagtc
      841 cggatgaagt ggcggcggaa gtgtggagca ttcttgaagg attcacaaac atcacaggct
      901 gaccgtgcgc tggaatattt ccgggcggcg gctgaaagcg atcgtctttc gcatgcgtgg
      961 ctggtggcct gcacggatat ccttgacggc agcgggtttg ctgagaaggc gctggccttt
     1021 cttttctgcg gggggctgat caagccctgc ggcgactgcc cggcctgccg gcaggtggcc
     1081 gcccataagc atgccgacat ccagtgggtc gaacccgtcc tgaagagccg tcggatcagc
     1141 attgagcaga tgctcgaggt caacagccgg ctcgcgagaa gttcctacag cggcggatgg
     1201 aaggcggccg tgctgctcca tgccgaccgc atgaccgatg aggccgccaa cgctttcctg
     1261 aaaacgctgg aggagccgtc cggccggacg atactttttc tggtcaccga ttccatcgag
     1321 gactgcctgc cgaccatcct gtcgcgctgt catcgggtcg cgcttcagcg gggagagcgc
     1381 ccggggacgg cggtctggat ggatgatctg ctggccttca tgcgcactct gccgtcgggc
     1441 ggctgtgcgg aaggcgccgc gcatgcggcg gagcttgcgg cgctgataga ccgcgaaagg
     1501 aagcggatcg agcaggaaat ggaggaccgt ccggctgacg atgacagcaa aaagtcgaaa
     1561 gagatattcg aggcgcgggt gagcgctgcg ctgcgccggg agcgttccga gatgctccgg
     1621 cagcttgcgc tctggcagcg ggacattctt tttgcatcac aggggctggg agacgaggcc
     1681 tttcatttcc gcggggatgc ggagagcatt cgccggcagg cgcaggatat cacctggacc
     1741 agcgcgcgcg cgcgggtgca ggctgtggag gacatgtccc gccgtctgga gcagaacctg
     1801 aacccgatca cggtgctgga acttgggatg gcggccctgg cggggtaaag cgctttacaa
     1861 aaacttgcgt tcccgctgga caggcccccg cgcccctgat agactcggtc cgctatgaaa
     1921 attctggttc tcaacggtcc taatctgaat ctgcttggca ggcgcgagcc cgatatctac
     1981 ggccgcctca cgcttgccga catcgaagag tccatgcgcg gccacgcccg gaagtgcgga
     2041 gtcgaggtgg atttcttcca gtccaacgat gaaggcgcgc tggtttcaag gatcggcgaa
     2101 gccgccggcc tctatgacgg cctcattttc aatccggccg cctacacgca caccagcgtg
     2161 gcgctgcgcg atgcgctgat ggccgtgaaa cttccctgcg tggaggttca tctttccaat
     2221 ccccagaacc gcgaagattt ccgccaagcc aacttgacgc tgggcgcctg catcggacag
     2281 gtctccggct tccgcgaccg cagctatatc ctggccatgg acggcctgat ccattatctg
     2341 aaaaacaaaa gctgaatttt gacatttaaa cgcaccccag ggagggacgc atgcagttaa
     2401 atgagataaa gaagatactc agtttgatga aggaaaacga tctcgccgag ttcgagatgc
     2461 aggacgggga ttcccgcatc catatcaagc gcggggcagg cggtgttccg gtgatgatgc
     2521 agccgccccc ggtaatgatg ccggcagccg ccccggctcc tgcggccgcg ccggccgccg
     2581 caccggcccc ggcccccgcc gccggcccgg tgatcaagtc cccgattgtc ggcacgtttt
     2641 accgctccgc ctcgcccgat gccgatgcgt ttgtttcggt cgggtccgag gtgaatcccg
     2701 acaccgtggt gtgcatcatc gaagccatga aggtcatgaa cgagatcaag gccgatgtca
     2761 aaggcgtgat ccgcaaggtg ctggtggaca atgccacccc tgttcagtac gggcaggccc
     2821 tttttgaagt cgaaccccgg taatctctat gtttgaaggt gttcttgttg ccaatcgagg
     2881 cgaaatcgcc gttcgcatca tccgggcctg ccgcgagctg ggcatccgtt cagtggccgt
     2941 gtattcggag gcggacgagc attcccttca cgtccagctg gccgatgagg ccatctgcat
     3001 cggtccggcg gccgcttcgg agagctatct gaaaatctcc agcatcatca gcgccgcggc
     3061 cgtggccaac gtggatgcga ttcatcccgg ctacggtttc cttgccgaaa atgccgcttt
     3121 tgccgaaatc tgccagaact gcaacatcac tttcattggt cccgatccgg agcacatccg
     3181 caagatgggc gacaaggccg tcgcgcgtga aaccatgatg gaggctggcg tgccggtcac
     3241 ccccggaagc gagggcatcc tgaagaacaa ggacgaggcc atcaagctgg ctcgttcaat
     3301 cggctatccg gtgctggtca aggccgtggc cggcggcggc gggaagggca tgcgcgtggc
     3361 gcacaatgac gtggcgcttg cgcaggcgtt ttcgatggcc agcgccgagg ccgagcgcgc
     3421 cttcggcaac gccagccttt atctggagaa attcatcgag agcgcgcggc atgtcgaagt
     3481 gcagatcatg ggcgacaagc acgggcagat cgtgcacctc ggagagcgcg actgcagcat
     3541 ccagcgccgg caccagaagc tgatcgagga aacgccctgt ccggtgctga ccgatgcaat
     3601 ccggaaaaag ctgcatgcca cggcggtgaa ggcggcggcc gcagtgggct atcacaatgc
     3661 cgggaccatc gagttcctct acgacgagct tgcgaaggaa ttctatttca tggaaatgaa
     3721 cacccgcatt caggtggagc attccgtgac cgaggaagtc accggtcttg atctggtcaa
     3781 ggagcagatc aacgtggcgg ccggacagaa gctttccttc acccaggagg aggccgagcc
     3841 gcaggggcac gccgtcgaga tgcgcgttaa cgccgaagac ccctacaaca atttctctcc
     3901 ctgtcccgga ctggtggaat gggtgcacct gccggcgggc cgcggcatcc gcgtggattc
     3961 gcacgtctat tccggttaca ccatctcccc gttctacgac agcatgatcg cgaaggtgat
     4021 cgctcacggg aaaacgcggc agcaggccct cgcgcgcatg cagggcgcgc tggaggagtt
     4081 catgatcaag ggcgtggaaa ccaccgcgcc gctcggccgc gcgctgatga ccgatgagca
     4141 tttccggaaa ggccgctaca acacgcatta cctggaaaca ttcatgaacg aaagcttcct
     4201 cctgaaatca tgaccatgaa aacgctgaaa atactgctgg cacttgtgcg ggggctcctg
     4261 atggcgctgg gcatcggatg ccttgtgtat gcgccctttt ccgggggatg catgggcggc
     4321 ggactgacgt ttttccgcga gcagtatgtg gcccttattt ttgtgctgct gctggcggga
     4381 ctgctgctcc tgccgggccg gatccggctc cgcgggaaaa aagagatgga gttcatcccg
     4441 ctgaacgggc ccggcggtga catccgcgtc agtgtgcggg tggcaaagaa cacggtccag
     4501 gaatgcgcgg cgaaatttga cgccatccgc agcctcgaac cggagctttc atggagttcg
     4561 gaagggctgt ccgtcgtgat gcgccttcag atgcagcgcg gccggccttt gaccacggtg
     4621 tgcatggacc tgcaggaggc cgtgcgggaa attttcgccg gggaactggg catcgagcag
     4681 ctgcgcgagg tgcgggccga tattatagaa ctttccgccg ccgcgaaggc cgccgaaacg
     4741 ctgcccgtgc cgccgcctga ggtggaatcc aaaccggagc agggggcctg acatggactg
     4801 gaaccgcatg gcggaggaat atcttggcgt ggccgacgag ctcttcaacc ggctgaaggg
     4861 cgaaatcgca gacctggccg agctgatcgc cgcgcggttg aaggcgggcg ggaaggttat
     4921 gatttgcggc aacggcggaa gcgcggcgga tgcccagcat ttcgcggcag agctggtcaa
     4981 ccgcttcctg atggagcgca gaccctatgc cgcgatcgcc ctgagcacgg acacctccat
     5041 cctgacggcc gtgggcaacg actacggcta tgaattcgta tttgagaaac aggtgcaggc
     5101 gctggggaaa aagggcgaca ttctgatcgc catttccacc agcggaaacg catccaatgt
     5161 ctgccgcgcg gttgacgcgg caaaagccct gggggtcacc accttggcgc tcaccggcgg
     5221 ccgcggcgga aaactggccg ggcttgcgga tcatgtgctg tctgttgcct ccacttcctg
     5281 cgtgccgcgc attcaggagg ggcatcagtt catcatgcac gcggtctgcg agcgcgtgga
     5341 agaaatcctc aaataaccgt tggcgggagc gcatcatgcc tgaaagaaca gtggatattg
     5401 tcattgtggg gtctgtgggc attgacacgg tcgaaacccc ctatgcccgc cgcgagtcca
     5461 ttctgggagg ttccgccagc tacgcctgca tggccgcttc ctatttcgcg ccgacgggca
     5521 tggtcggcgt ggtcggggcg gatttcccgg aggcgtaccg aaaactttat gcggatgccg
     5581 ggatcaatct ggacggcctg cggacggttc cgggagacac cttccgctgg gagggaaaat
     5641 acgaagccaa catggacaac cgcagcacgc tggcgatcgc gctgaatgtg ttcgaggact
     5701 tcaagcccga actgccggcc ggctatgtga acgcgccgta cattttcctc ggcaacatct
     5761 cccctcagct tcagatgcat gtgctggatc aggtgaagtc gccggagttt gtgctggccg
     5821 acacgatgga tctctggatt caaaccgcgc cggatgatct gaagaagctg atctcgcgcg
     5881 tggacatgct gaccctgaac gaatcggagg cgcgcgcgct gaccggcggg cgctattcgc
     5941 tgatccgcgc gggccgcgac ctgctggagc tgggcccgaa atatgtgatg atcaagaaag
     6001 gcgagcacgg aagcctgctc atcacgcgcg acaatctttt tgtgcttccc gcgtttccgc
     6061 tggagagggt ggtggacccg accggggcgg gggacacctt tgcgggcgcg tttctcggcg
     6121 cgctggctcg gtccgggggc atcagcgaaa aaaatcttcg cagggccatg cgctgcggcg
     6181 cggtcgtggc ttccatcggc atcgaggcgt tcagtctgga tgcgctcagg catctgacgc
     6241 tggctgaaat cgaaaagcgc gtcagccagt ttcgcgcata cacagcgatt gactgaacac
     6301 gctccgcgag ccatcttgcg gccttctcct tttcgcggct tcaccggtct tcgatggtcc
     6361 ccaaaaaccg ccgaaaaaag tcaaaaaaca ccaaaaaagc ggcaaaaatg gccaaaaatg
     6421 gcaaaaaata gccaaaaatg agcgaaaaaa cagaaaaaac gcaaaaaatg gcagaatatt
     6481 gtcaagaaaa atatgaaaat atcgaaaatc gccgatattt ttatgctgat tttgcggaat
     6541 tcataaaata atgaaaaaat agcaaaatat ttttaatatt attcttgcac actccggatt
     6601 ctgtggtaaa tatgcaagta cttttgagtg acagctcgaa gagaggtacg atatgaccaa
     6661 gacaaccatg gatggcggcc aggcagtgat taaatgcctt gaaaatgaag gcgtggaatt
     6721 tctgttcggc atttccggcg gttcggcaat cccgattttt gacgcgattg tcaccaccag
     6781 caccagcatc aaaatgatcc tcacccgcca cgagcagggt gcggcccaca tggccgatgg
     6841 ctatgcgcgc gccaccggga aacccggcgt ggtgctggtc accagcggcc cgggggcgac
     6901 caacacgctc accggcatca tgaccgcgca catggacagt gtccccatga tcgtcctgac
     6961 cggccagtcc atccgtccga tgattgggaa ggatgccttt caggaggcgg atatcttcgg
     7021 gatgtgcatg ccggtcgtga agcacagcta tctgatcaag gacacgaatg acatcccgcg
     7081 gatcatgcgc gaggcgtttc atatctgcaa caccggccgc ccgggcccgg tcctgattga
     7141 tctgcccaag gacgtgaccg ccgggccctg cacggcggac ctcgccgcgg aaatggatct
     7201 gcccggctac aagctgcccg atccgcccga caaggaaacc gtgctcgaaa tcgccgaggc
     7261 cctcagcaag tccgagcggc cggtgatgat gctcgggcac ggcgcgctga tcgccggcgc
     7321 cgcggaatcc gcgaaaatcc tggccgagaa aatgcgcata ccggtgatga ccaccttcct
     7381 cgggaagggg gcgttcccgg aaacgcacag gctctcgctg ggctggccgg gcatgcacgg
     7441 cacggcctat gccaacaagg cgctttgcga atgcgacctg ctgatcaaca tcggcgggcg
     7501 cttcgatgac cgcattctcg gcaagcccgc cctgttctgc aaggacgcag tcaaaatcca
     7561 tgtggacatt gatccggccg aaatcggcaa gatgatcagg ccggacatcc actgcgtctc
     7621 ggatgcgaag gatttcattg atgagctcac aaagcacgtg gggcacctga atacgggcga
     7681 gtggctggcg aaactggacg ggtataaaaa gaaatatccg ctcaagtacc gcaagggcgg
     7741 cggcctcaag atgcagcacg tgatcgagga gctgtataaa atcaccgggg gcaaggccgt
     7801 tgtcgccacc gacgtgggcc agcaccagat gtgggcgggg cagttctata aaacggacta
     7861 tcccaacaac tggctgtctt ccggcggggc cggcacgatg ggcttcggtg tgcccgcagc
     7921 cgtcggcgcc cagttcgggc ggccgaacga gctggtggtc tgcatcaccg gcgacggcgg
     7981 tttccagatg tgcaatcagg aaatgaccac ggcagtggtc cacaagctcc ccgtcaagtt
     8041 catcgtcctg aacaaccact atctcggcat ggtccgccag tggcaggaga tgttctatga
     8101 cgaccgcaag agcggcgtgg acctggaggg caaccccgac ttcgtgaaac tggccgaggc
     8161 ctacggcatg aagggcttca acctgaagcg ggcgggggac atccgcaaga tccttaaaaa
     8221 ggccatcgag tggaacgacg ggccctgcct catcaacgcc gaagtggaga agaccgacaa
     8281 cgtgttcccg atggtgcctg cgggcgccgc gctggaggac atgctgattg aacctccgcg
     8341 gcacaaactt gaaaagccga ccggttccac gtgatcgaaa aaggagtcac tgcaatggca
     8401 aacacagaat tgaacaattc gatcggggtg cacaccctca gcgcctatgt cgccaacaag
     8461 cccggcgtgc tggcccgcgt ggcgcaggtt ttctcgcgcc gggggttcaa cattgactcg
     8521 ctcgtggtgt cgtcctccat tgacggagat ttctcgcgca tgaccatcac cgcgaccgga
     8581 aatccggaca tgctggatca ggtcatccgc caggtgaaca agctggtgga cgtgatccac
     8641 tgcttcgacc acaccggcca ggatgtcgtc gtgcgggagc tggcgctcat caaaatcggc
     8701 gtcaccgccg aggagcgcac cgaggcgctg caggtcgccg agcactttgc ggccaagacg
     8761 ctggacctca cggaaaattc gatgatcatc atgtgcactg gccggtcgag caagctcgac
     8821 gcgctgaccg gcctgctcaa gaaatacaaa atcatcgagc tcgtgcggac cggcaaggtc
     8881 gtcatggccc gcggcgacga tccgacctga tgcagaactt ccaatgattg gaactttttg
     8941 cgcgaaaagt tccaatcatt ggaaaaatcc cctctaacag ttccaattgc tggaactatc
     9001 acgcgtttac agcaccattt tgacgccttc gatgctgcgg agttcggcgt caattttgtc
     9061 catgatggcc ttcgattcca cgcgcacgga gaggttgctt gtgatccagc cgctgacagc
     9121 ataatgtata tttgcattct aaatgcaaat atgtcttgac tgaaaacggg gatgcgtggc
     9181 ataggtggag tcatgaaaaa atatgttcat cgaagcctcg agaccgtcct gaagaacctg
     9241 ctgaagcagt tcccggcggt ggctgtcacc ggtccgcgtc agtccgggaa atccacactg
     9301 cttctcaaaa cgcttccgga ggcccgctac gtgacgctcg acgatcccgt tgcgcttcag
     9361 caggccctaa acgaccccga actggttctg ggaaacggcg acaaaccggt tgttctggat
     9421 gaaattcaat acgctccatc actgcttccg tatatcaaaa tgcggattga ccgcaaccgg
     9481 ggcgccaagg ggcggtttat tctgaccggg tcgcagcagt ttgccctgat caagaacctg
     9541 ggggagtctt tggccggacg cattggcttg cttgagctcc ttccattcag cgttgaggaa
     9601 gcttcgcggg ccggcgcaag ttccggttct ctggcccgtt ttgagcgggc ctgcctgcgg
     9661 ggcatgttcc ccgagccaac catgaattcc ggaatggatg tcccccggtg gtatgcctcc
     9721 tatgtccaga cctatctgga acgtgacatc cgcaccctgt atgacatcgg gaacttgcgt
     9781 gaatttgaac ggttcctgca gttgcttgcg gcgcgatgct cgcaggaact ccatcttacc
     9841 gttcttgcga cggataccgg ggtggcggtc aataccatca agaaatggat ttcaattctc
     9901 gaagcctgca gaattatata tctgcttcct ccctattaca ggaatctggg caagcgcatt
     9961 gtcaaagccc cgaaggtata ttttctggat atcggcatgg tgtgctatct gacgggcttg
    10021 cgggatcggc atcatgtcat taacgggccg ttggctggcg ctctgtttga aaatttctgc
    10081 gttcaagaag cgctgaaggc gatgctttca aaaggggttc cgccccgttt gtatttcctg
    10141 aaaactcata acggactgga agttgatctg ctggtcgagg gcattaacgg ccggctgtgt
    10201 ccgtttgaat tcaaattctc aagcacgcct aagcctgcca tgtgcagatc gctggagcgt
    10261 tttggcgaac tgttcaagga tttgaatccg gaacccggca aattggtttc cctgtcggaa
    10321 aagacggctc cgctgacgcg ttcaattatg catgcttcac tggacgtatt cctctcggag
    10381 atcaggaatc tggcgcaagt ctagagcatg ttgaaaatga atatgttctt cacagcacca
    10441 gcttgacgcc ttcgatgctg cggacttcgg tgtcaatttt gtccatgatt tcccgggaat
    10501 ccacgcgcac ggagatgttg taggtgatgt agttcccatt ggccgaaacg ttgccggcct
    10561 ccagcaaatt ctcgttgcct gtatttctgc ttgactgacc cgttttttgg tagcagggtc
    10621 agttcatgag cctgttctgg tccattggct gtggtggtcg catacgtcag cgcaggaggc
    10681 atcggttttg tatcgggcga atgtgttccc ctgtaacctt tctgattgcg gcttgtgttt
    10741 tctctataat ggtcggtcta ctggatttgc gtgctgagga tttggctgct cctgcgacgc
    10801 ttcagttcac ttcgccgact cccgcatctc agctctcgac gatcggaacc ttctattcgg
    10861 cacagcatcc tgactggccg cctttccctg caaatgtgca tgatctgcct gcatgggccc
    10921 tgggcaatgg ctgctttttg cttgccgatg cggactttga ctacgaggcc tttgcagaat
    10981 caatggaagc cgtgaattac acgtccggca aatatccaat gatctcccgg gattactcca
    11041 gcgacgacct ctggattaca atcaacgcgg ttgtcaatgg acgcaccgat ttcacggccg
    11101 atttgactat ccataccccc gagaccagta cctgcgccag ctgggatttg tattatgcgc
    11161 gcgctctcga ttggcagacg gattggaaat ttctgatgcg atgtcccaca aatatcattg
    11221 ggcagaccaa tgtgtatgcg cgatacatgc ccgatgaaat agcgttcttt atggcgagag
    11281 aaacaacgac caactgtgac ttgagcgtca cgaccaatgt cacgccgcag cagatggccg
    11341 aaatgctggt tccgccttgg gtgacggtaa caaacgttaa ttacaccggc gccgccgtcg
    11401 cgcgagggat cttcgcgaac ggcaattgtt gcggcctgcc gatcgcggga ggcgtgattc
    11461 tctcttcggg atatataacc gatgcggctg ggcccaatga tgttgacaat aaaggggata
    11521 ctgtgggcgg cccgcctgat gccgacctga atcttctcgt cggcggctcg gatagcaagg
    11581 acgctgcaat cctggaattc gatgttattt caacaaacgc atgtctgctg aaattcagat
    11641 atgtctatgc ttctgaagag tatccggagt attctgggtc aatgtactgc gacccctttg
    11701 ccatttttgt tacaacaaat cgtgtgggtg cgaactggat taatactata agcaacaata
    11761 ttgcccttgt acccgttgat gcacatattc cggtaaccgt ctacacggtt aacggtggtt
    11821 atgagcatgc atacgaagga gttatcccca ccaatgcgcc gtactatgtg gacaatcacg
    11881 acccctataa ccaagctgtg tcgccatatt taatcgggga gccggtgttt aacatccagt
    11941 atgatggtat gaccgtgctt ctcgcagccc aaacattcgt tgacgcctgc acaacgtctc
    12001 atatcaagat tgcgatttcg gattataccg actcatatta tgattcggcg gtcttccttg
    12061 atgagtggtc acatgataat tcgcagtaaa aggagggaaa cccatgcaga agatcattta
    12121 tactcttatt gcgagcgtgt gtatctcttg ggcgttgagc aaaccagcct gcggggacct
    12181 ctgggtttcc aacctggacc aatttccaat cggaagtgcg gcggttggca gcgacttctg
    12241 gatagctcag agcttctata ttgatataag cgactcaaac cagtacgcgc ttgacgctgt
    12301 tcagctcctg atgaatccgg caaccggcag cccaaatggc tttgctgttt ccatcttcag
    12361 cgctccatct gacggccttc cgcaggacta tctcggaagc cttgctggtt ccgcaacccc
    12421 cgtgaccgga ggcctctaca cgtacacggc ctcgggcatt acactttctg cgggcatcta
    12481 ttccgttctt gttaccagct caacgtccac aaatcagggc gcgttcaact ggagcgccag
    12541 cgacagatcc actcagaacg gaacatggaa catcgccagt gtgtactaca cgtcttcaga
    12601 cggtttgaat tggcaagaga atggtcgcgc agatgtcttt cagatggcga tccatgcgac
    12661 cattgtgccc gagccggcaa cggtatggct gctgggtgcg ggtttgtttt cgttaagcct
    12721 ctgctgccgg cggaagccgc gcttcacagc accatcttga cgctttcgat ggcgcgcagt
    12781 tcggcatcaa ttttatccat gatctcccgg gaatccacgc gcacggagag gttgtaggtg
    12841 atgtagttcc cattggccga aacgttgccg gcctccagca aattc
//
LOCUS       DAOQWR010000067        12874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5366_length_12874_cov_6.327639, whole genome shotgun sequence.
ACCESSION   DAOQWR010000067 DAOQWR010000000
VERSION     DAOQWR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5366_length_12874_cov_6.327639"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="pstC"
                     /locus_tag="PKK36_06715"
     CDS             <1..796
                     /gene="pstC"
                     /locus_tag="PKK36_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNR94286.1"
                     /translation="SLFLKSLPVLKMQSLGAILFSTEWHPMQGEFGMLAYIMGTATVT
                     GLAMLIAVPVSLLAAIYLAEYSPARFRQMVLPLIDLLSGIPSVIYGVWGIVVIVPAAR
                     WLSQRFGAWSSGYCLLSGALVLAVMVCPFIIHVAREVLSTVPFGIREASLSLGATKWQ
                     TVKLTVLRKALPGIVAAVILGLSRALGETIAVLMVIGNVPAIPKSLFDPAYPLPALLA
                     NNYGEMMSVPLYDSALLMSGFILLVTVLIANLAAKGILRAMQRGQI"
     gene            793..1659
                     /gene="pstA"
                     /locus_tag="PKK36_06720"
     CDS             793..1659
                     /gene="pstA"
                     /locus_tag="PKK36_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010421612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNR94287.1"
                     /translation="MKRSNLAARKIEEGIFKGLMYFSVALVLGTLLLITGIIIAKGLP
                     SLSIQMLTQTPKGGYYLGKEGGILNAIIGSIYLATGATLLALIISLPVSLYINVYTRS
                     NSKLAALVRFSMDILWGIPSIVYGAFGFIIMISLGLRASLGAAIIAVSLFELPIMVRA
                     MDEVLKMIPPELAEASYSLGATRTETAFRVIVRQAIPGLITGVLMAFGRGIGDTASVL
                     FTAGFTDRIPTSLSQPAATLPLAIFFQLGTPFPEVQGRAYAAATVLTIMVLIISLTAR
                     LIAHRYSKCITR"
     gene            1669..2427
                     /locus_tag="PKK36_06725"
     CDS             1669..2427
                     /locus_tag="PKK36_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNR94288.1"
                     /translation="MSTRNLSIENLNVWYGDRRILHDVTLDIPDRQITAIIGPSGCGK
                     TTLMRCMNRLLEVNTDSRIEGKVLVDGRNIYDPGTEVTSIRKKMGLLSQRPQVLPMSI
                     YDNVAYGLRIHGLRKKSELDRIVEKNLRACSLWDEVSDRLNSPATGLSIGQQQRLCLA
                     RGLAVEPEIILGDEPTSALDPQASQNIEQLLVQLKKDYTIVLVTHILRQAKRIADHVV
                     FLYMGKVIETGPTGDVLNNPREELTKRYIAGVIS"
     gene            complement(2885..3835)
                     /locus_tag="PKK36_06730"
     CDS             complement(2885..3835)
                     /locus_tag="PKK36_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR94289.1"
                     /translation="MKILVADKLSDTASKELTALGAEVISKPDLSADELPAAIGDAEI
                     VIVRSTKVTKATIDAATALKVIIRAGAGVNTIDCAAAKAKGVFVSNTPGKNTDAVAEL
                     AIGMMIAADRRIVNATADMRNGVWNKKEYGKARGLKGRTLGVMGMGAIGKAVASRAKG
                     MEMNIVAWDIDLTPEKAASLGVGHCATKLDLAAKSDVVTVHIAAVPATAHFFNKEFFA
                     AMKKGAIFINCARGEVVDTAALKAAIKEKGLRVGLDVYENEPAAQDKIFADKELASMV
                     MGATPHVGASTDQASEAVAEEVVNIVKAYKATGKPLHAVK"
     gene            complement(4003..4575)
                     /locus_tag="PKK36_06735"
     CDS             complement(4003..4575)
                     /locus_tag="PKK36_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94290.1"
                     /translation="MQKGALMAAETFTVRVNRLILLFLGLVGALFFRVGLDGAFFREW
                     LNWNFPPGISWQFIAFCFFFLGCGGLIALNCLWYFIRPPVMLRFDEKGVTFGTGFRYR
                     PHTIAWKHVEKIGYGAEACVTLKAQLFAGAAVVFRDDPGVPAFRAVSMGLGYSFRRLT
                     LHWLYADRLPWTIVREGKSIFKKYTSEPCA"
     gene            complement(4575..5618)
                     /locus_tag="PKK36_06740"
     CDS             complement(4575..5618)
                     /locus_tag="PKK36_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94291.1"
                     /translation="MKKMLTVFLLIFAAKGTLAGDVKILFDDIDDRFLLNAGSNTFMS
                     SGYSTGTVTLLDQQQTNKEWTGSAYLTAEHQTFTAGRAGRISHVSVCCPPPVHGTMRL
                     HRGSDRMVNREWEKNVVITQEWQVIPVDTVVMVSSGEVMSLVIDPGEPPNYFDFWWIG
                     SNSYLQGDFSYTWPADLCFKTWISDTSAGTEFDGTVTADAFVGDGSGLTGLGDADTLD
                     GYHASSFPRRRLSGFINAGTSTNILVPHFYSFTLLLACEHPHVNGIANLHGFENDGYM
                     VITYTKYDGTGAGIAGAGEGHVTSTTNLLTFGSGSVYYVRCPGMSTGSNQPQYLSLVA
                     TNASLGILYQLIY"
     gene            complement(5615..6430)
                     /locus_tag="PKK36_06745"
     CDS             complement(5615..6430)
                     /locus_tag="PKK36_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94292.1"
                     /translation="MSVSSGMIVNDASISASKVYIASNSTLAGSGAVQGSLAVAGTVA
                     PGGRAPGTQTVFGSIAFISGSRFDCDVKSQSALDCLSASGPVTGLCRVSLNPSPGAYP
                     IHQMIVLGNASSSYAGFTPDNTWLWRLESTGSLNLAVTHLRGDTDSDGLPDWWESDHF
                     GGRTNAQPSAHSDADGVDNRSEYIANTDPLDAGSALELTDIQRMPESDDVIEWSTAVG
                     RRYYLLVHTNLPRTSWSTAAVITVYGAPLQAWTNTAAGSASLFYEVAVEENAP"
     gene            complement(6584..7099)
                     /locus_tag="PKK36_06750"
     CDS             complement(6584..7099)
                     /locus_tag="PKK36_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94293.1"
                     /translation="MKLHGDITMNGKHFAKGSEIPWYSVYPFFLIHMLAFGASGFAMA
                     YAKSGPPVAFLYAHGGFAILVYTVFYLAIFGLDEVKWMFINAGLGLLGIYTQIGWILS
                     LFKKEISDYPLHVHVIPFLYFVLYTFLLRQAVLDFTHSREDAPRKHRVEFGYVAVSIA
                     VYLFSWYLESR"
     gene            complement(7133..7870)
                     /locus_tag="PKK36_06755"
     CDS             complement(7133..7870)
                     /locus_tag="PKK36_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94294.1"
                     /translation="MTMRIIAAFLCLIGLTRASSAQHTLDLEAGYFFPGYNDVAIPGD
                     SGTRLSLTDDLDADGDVVFRVRYGCTISDRHWVGFLAAPLTMESAGVLDRDTLFDDSL
                     FPAGSPVEATFRFDSYRMIYRYIFRETDRWKICFGGALKVRDASIQLEGGGMAAEKSN
                     TGVVPLLSFNVTWTPFEKLHLLMDGEALAAPQGRAEDVMFALQYDVSPRLALQAGYRI
                     LEGGADNDEVYTFSLFHYAVAGLVLSF"
     gene            complement(7867..9117)
                     /locus_tag="PKK36_06760"
     CDS             complement(7867..9117)
                     /locus_tag="PKK36_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR94295.1"
                     /translation="MNLNNSESGAQGVHKRSMLGLLGMVVLVGMGEHMAERFLPLYLQ
                     ELAQASTAILAIGILAGMDDLLSALYSFPGGYLSDRLGTKRALLVFNVLSMAGYLCVI
                     FIPAWWAVLLGAALFISWSAISLPATMDLLARVVPKHRRTLGVSALALIRRVPKMLGP
                     VAGGACIAIWGVERGVRAAFIAALILALIAAVMQQMLIVDDEKRARAAEPNPLRLWRE
                     MPRGLKNLLWSDILIRFCERIPDAFVVIWATRHIVRPVTELSFGWLSAIENMAAVLIY
                     LPVAYMADHFGKKPFVLITFVFFTLFPLALLHSQSLAPLIAAFVLRGLKEFGEPSRKA
                     LIMDLAPEGRKAAMFGLYYLFRDVLVAFAAFGGALLWRISPELNLKAAFAFGIAGTLL
                     FWWKGRDAEMSGLQTALEMNNEEQ"
     gene            complement(9186..9890)
                     /locus_tag="PKK36_06765"
     CDS             complement(9186..9890)
                     /locus_tag="PKK36_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR94296.1"
                     /translation="MKARYAWAFSLGLLLAAAGCQSAGLLPKSFPLNEENRKWLQEGE
                     QTRITPAVRAAAKPLLAGSSRGQKIRIVMDLLHSEFTYDQAESIHAAARTADELVRGG
                     VFGDCTDYALVAAALLRSAGVPARIVLAADAGWIGLLRRDPSAVPEGHAMLEVWGDRG
                     PQWINPSWGNLYPVRPGGGNVLPDGYLWARAGRDYWDMGIRGVEDLRNLLYETARGAD
                     REETEQASFGAPVPRN"
     gene            complement(9912..10649)
                     /locus_tag="PKK36_06770"
     CDS             complement(9912..10649)
                     /locus_tag="PKK36_06770"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNR94297.1"
                     /translation="MMTRYQISLAGLVVLFCFSLLSGQMVFGQQAIGSVVALEGGAVA
                     VRASGMEEELGSGADVFLNDRILTRRRSRITIRFLDESTLSQGESSEMVLNEFVFNPA
                     RKKDNAFQVRMIQGAFRCITGKIVEMNPARFGLRTGKMTIGIRGTDIGIEINPYTGEE
                     NLLVFDLHRRELVVAPGVAGLEPFVVTTPFVQFQVTEKGVEQRALTQAEAKRFIKKSA
                     PKGAPPPGGPTPASGVKMDMPPWLENR"
     gene            complement(10763..11938)
                     /locus_tag="PKK36_06775"
     CDS             complement(10763..11938)
                     /locus_tag="PKK36_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNR94298.1"
                     /translation="MKPRTISDRVQWVGAVDWNRRLFDSLIPLPDGTSYNAYLVRGSE
                     KTALIDAVDPALQHLLFAQLADVPQIDYVISQHAEQDHSGALPDVLKKYPGAKLLCSS
                     KARGLLLEHLALPEDRVQTVEDGETLSLGDRTLQFVYTPWVHWPETMSTYLPEEKILF
                     SCDFFGSHLATSDLYAGGSAYVIEAAKRYYAEIMMPFRTVIQKNLQKLEPLDFNMIAP
                     SHGPIYDQPQSILDSYRDWVSDRVSNMVVIPYISMHGSTEMMVNHLMQALIEREVGVQ
                     KFDLSVTDIGKLAMSLVDAATIVIGTPTVHVGPHPVVYSAAQLANAIRPKLKFAAVIG
                     SYGWGTKAVEQISGLIPNLKVEVLGAVMCKGLPRAESFAALNELAGKIRDSHQRVAG"
     gene            complement(11964..>12874)
                     /locus_tag="PKK36_06780"
     CDS             complement(11964..>12874)
                     /locus_tag="PKK36_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR94299.1"
                     /translation="RELLGVLPGHDAFEFRYQARLPEISGPARCWLPIAQSDDFQNVT
                     VKSVRAPGTRKMLTDQAHGNRVMFLELGPAASGKEIEIVYDVERLEKAAYAGDPAEAE
                     KHLRPERMVPADETIRAAAEKAVEGREGALMQARALYDHVIDQMRYMKFGEGWGQGSS
                     VYACDALSGNCTDYHSYFIGLARAVGIPARFAIGAGIPSERDEGGTDGYHGWAEFYAE
                     GKWWPVDVSEADKFSALSMYYFGHHPANRFEFSRGRDLELDPAPASGPVNFLAYPLLE
                     VGGVPRGVKTMFLFRRTSCRADGPKT"
BASE COUNT         2801 a         3954 c         3581 g         2538 t
ORIGIN      
        1 cagcctcttt cttaaatcgc tgcccgtgct gaaaatgcag tcgctcggcg ccattctgtt
       61 ctcaacagag tggcacccca tgcagggcga gttcggaatg ctggcctata tcatggggac
      121 ggcgaccgtc accggcctgg caatgctgat cgccgttccc gtttcgcttc tggccgcgat
      181 ttatctggcg gagtattcac ctgcgcgctt tcggcagatg gtccttccgc tgattgatct
      241 gctgagcgga attccctccg tgatctacgg ggtctgggga atcgtggtga tcgttccggc
      301 cgcgcgctgg ctttcgcagc gcttcggcgc gtggtccagc ggatactgcc tgctctccgg
      361 ggctctggtg ctggccgtga tggtctgtcc gttcatcatt catgtggcgc gcgaggtgct
      421 ctccacagtg ccttttggca ttcgcgaagc ctcgctctcg cttggcgcca ccaaatggca
      481 gaccgtcaaa ctgacggtgc ttcgcaaagc cctgccgggc attgtggcgg cggtgatcct
      541 cggcctttct cgcgcgctcg gtgaaacaat cgccgttctg atggtcatcg gcaacgtgcc
      601 ggccattccg aaatctctct ttgatccggc ctatcccctg cccgccctgc tcgccaacaa
      661 ctacggcgaa atgatgtccg tgcctcttta tgattccgcc ctgcttatgt cgggcttcat
      721 tctgctggtc accgtgctga tcgccaacct ggccgccaag ggcatcctgc gcgccatgca
      781 aaggggacag atatgaaacg ctccaatctc gccgcgcgga aaatcgaaga gggcattttc
      841 aagggcctga tgtatttctc cgtggctctc gtgctcggca ccctgcttct gatcaccgga
      901 attataatcg ccaaaggact cccgtcgctc agtatccaga tgctgaccca gacgcccaag
      961 ggcggatatt atctcggcaa agagggtgga atcctgaacg ccatcatcgg ttcaatctat
     1021 ctcgcgacgg gagccacact gctggcgctg atcatcagcc tgccggtgag cctctatatc
     1081 aatgtctata cgcgaagcaa ttcgaagctg gccgcgctgg tgcgcttcag catggacatt
     1141 ctctggggca tcccctctat tgtctacggc gcgttcggat tcatcatcat gatttccctc
     1201 ggcctgcgcg cttcgctggg cgcggccatc atcgcggtca gcctttttga actgccgatc
     1261 atggtgcgcg ccatggacga agtgctgaaa atgattccgc ccgagctggc ggaagcctcc
     1321 tacagcctcg gcgccacccg cacggagacc gccttccgcg tgatcgtccg gcaggccata
     1381 ccgggactca tcacgggcgt gctgatggct ttcgggcgcg gcatcggcga caccgcctcc
     1441 gtgcttttca ccgccggctt caccgaccgc attcccacat cgctttcaca gccggccgcc
     1501 accctgccgc tggctatttt ctttcagctc ggcacaccct tccccgaagt gcagggccgc
     1561 gcctatgccg ccgcaaccgt tctcaccatc atggtgctga tcatcagcct gacggcccgg
     1621 ctgatcgcgc accgttattc aaaatgcatc acccgctagg agcaagccat gagcacacga
     1681 aacctgagca ttgagaacct gaacgtgtgg tacggcgacc gccggatcct gcacgacgtc
     1741 acgctggaca ttcccgaccg gcagatcacc gccatcatcg ggccttccgg atgcgggaaa
     1801 accacgctga tgcgctgcat gaaccgcctg ctggaggtga acaccgactc ccgaatcgag
     1861 gggaaagttc tcgtggacgg aaggaacatc tacgatcccg gaaccgaggt aacctccatc
     1921 cgcaaaaaaa tgggccttct ctcgcagcgg ccgcaggtcc ttccgatgtc catctatgac
     1981 aacgtcgcct acggccttcg cattcacggg ctccgcaaaa aatccgaact cgaccggatc
     2041 gtcgaaaaaa acctccgggc ctgcagcctc tgggatgaag tgagcgaccg gctgaacagc
     2101 ccggccacgg gtctctcgat cggccagcag cagcggctgt gccttgcgcg cggcctcgcg
     2161 gtggagccgg aaatcattct gggcgacgaa ccgacctccg cgcttgatcc gcaggccagc
     2221 cagaacatcg agcagctgct ggtgcagctg aagaaggact acaccatcgt gctcgtcacc
     2281 cacatcctgc gccaggcaaa acgcatcgcg gatcatgtgg tgttcctcta catgggcaag
     2341 gtcatcgaaa ccggaccgac cggcgacgtg ctgaacaatc cgcgcgaaga actgaccaag
     2401 cgctacatcg ccggcgtcat cagctgaaga agaaagcgcc gtcgcggcgg gtgcgcactt
     2461 cccagtggcg ctccgaatca ctctctctgt cgctcgcgca cttcgtgttg ccactgcatt
     2521 caagtcgtca atatagtact atataatata tcggtatccg atatatcgga taccgatata
     2581 ttgttccgag agttgtcatc tgcaagcgca gcgccacgcc gctttatgga gggatgggct
     2641 ctgttcgccc gtcgaagcct ctgggcgaag tcgggtcccg tccgcttcca tccaattcgt
     2701 tctccggccg gcacggaggc cggccctcca gaagacgtgg agggatgggc tctgtccgct
     2761 cgacgaaacc tctgggcgaa gtcggggccc gtccgtttcc ggatgcgccc ccggcgggct
     2821 tgtccctgaa aaagaaaggg ccgcgcaaac gcgcggccct tcttttcacc atggtccgga
     2881 cggcttactt caccgcatgc agcggcttgc cggtcgcctt gtaggccttg acgatgttga
     2941 cgacttcttc cgcgaccgct tccgaagcct ggtccgtcga ggcgccgacg tgcggggtcg
     3001 cgcccatcac catcgaagcc agctccttgt ccgcaaagat cttgtcctgc gccgcaggtt
     3061 cgttttcata gacatccaga ccgacgcgca ggcccttctc tttgatcgcg gccttcagcg
     3121 cagccgtatc caccacttcg ccgcgggcgc agttgatgaa gatcgcgccc tttttcatgg
     3181 cggcaaagaa ttccttgttg aagaagtgcg ccgtggcggg caccgcggcg atatgcacgg
     3241 tgaccacatc ggatttcgcg gccagatcca gcttcgtggc gcagtggccc acgcccagcg
     3301 aggcggcctt ctccggcgtc aggtcaatat cccacgcaac gatgttcatc tccatgccct
     3361 tcgcgcggct ggcgacggcc ttgccgattg cgcccatgcc catcacgccc agcgtgcggc
     3421 ccttgagccc gcgggccttg ccgtattcct tcttgttcca cacgccgttg cgcatgtccg
     3481 ccgtcgcgtt cacaatgcgg cggtcggcgg cgatcatcat gccgatcgcc agttcggcca
     3541 cggcatcggt gttcttgccg ggggtgttgc tcacaaaaac gcccttcgcc ttcgcggcgg
     3601 cgcagtcaat cgtgttcacg ccggcgccgg cgcgaatgat gactttgagc gccgtggcgg
     3661 cgtcgattgt cgctttggtc accttggtcg agcgcacaat cacgatttcc gcatcgccga
     3721 tcgcggcggg cagttcgtct gcgcttaaat cgggtttcga aatcacctcc gcgccgagcg
     3781 cggtcagctc tttggaagct gtgtccgaca acttgtctgc aaccagaatc ttcattcttc
     3841 aatcctccat atttgcctga taaatccgca atgtgccgcc ctgtataacg ccaaccccgc
     3901 tctcctgcaa gcccgcgatt ttccaatcac tggaaatttt gtccgaaaaa gttccaacca
     3961 ttggaagttt tttcgcgaaa agttccaacc attggaagtt tttcaggcgc agggttcgga
     4021 tgtgtatttt ttgaaaatac tttttccttc ccgcacgatg gtccagggca agcggtcggc
     4081 ataaagccag tgaagagtga ggcggcggaa ggaataaccg aggcccatgc tcacggccct
     4141 gaaggccggc acaccgggat catcgcgaaa cacaactgcg gcgccggcaa acagctgggc
     4201 cttcaaggtg acacaggcct ccgcgccata gccgattttt tcaacgtgtt tccaggcaat
     4261 ggtgtgcggc cggtagcgga aacccgtgcc gaacgtgacg cctttctcgt cgaagcgaag
     4321 cattaccggc ggacggatga aataccacag gcagttgagc gcaatgagac cgccgcatcc
     4381 gaggaagaag aagcagaacg cgatgaactg ccacgagatg cccggcggga agttccagtt
     4441 taaccactcg cggaagaaag cgccgtcgag cccgacgcga aagaaaagcg cgccgaccag
     4501 cccaagaaaa agaagaatca gccggttgac gcgaaccgtg aatgtctctg cggccatcag
     4561 tgctcccttc tgcatcagta tatcaactga tacagtatac ccagcgaagc attggttgca
     4621 accagagaaa gatactgagg ctgattgctt cctgtgctca ttcccggaca gcggacataa
     4681 tagacggacc ccgagccgaa ggtcagcaga ttggtggtgg atgtgacatg gccctcaccc
     4741 gcccccgcga tgcccgcgcc ggttccgtca tattttgtat aggttatcac catgtacccg
     4801 tcgttctcaa aaccgtgcag gtttgcaatg ccgttgacgt gcggatgttc gcaggccagc
     4861 agaagagtga atgaatagaa gtgcggcacc agaatgttgg tagaagttcc cgcgtttata
     4921 aaacccgaaa ggcggcggcg cggaaatgat gaggcatgat aaccatccag cgtgtccgca
     4981 tccccaaggc cggtcagacc cgaaccgtcg ccgacaaacg catccgccgt cacggttccg
     5041 tcaaattctg taccggcaga agtgtcgctg atccacgtct tgaaacacaa atccgcgggc
     5101 catgtgtaac tgaagtcccc ctgcaggtag ctgttgcttc caatccacca gaaatcgaaa
     5161 tagtttggcg gctcacccgg atcaatgacc aggctcataa cctccccgct ggagaccatg
     5221 acgaccgtat caaccgggat cacctgccac tcctgcgtga tgaccacatt tttttcccat
     5281 tccctgttca ccatccggtc agagccgcga tgcaggcgca tggttccatg caccgggggc
     5341 ggacagcaca cactgacatg agaaatgcga ccggcgcgac cagcggtgaa agtctggtgc
     5401 tctgccgtga gataggccga accggtccat tctttgttgg tctgctgctg gtccagcagc
     5461 gtcacggttc ccgtgctgta accggaagac atgaaagtgt tggatccggc attgagcaga
     5521 aacctgtcat ctatatcatc aaacagtatt ttaacatcac ctgcgagagt tccctttgcg
     5581 gcaaatataa gcagaaatac ggtcagcatt tttttcatgg cgcattctcc tcaacagcaa
     5641 cctcgtagaa caaactggcg gaccccgctg cggtattggt ccaggcctgc aggggcgctc
     5701 cgtaaaccgt gatgaccgcg gccgtgctcc atgacgtgcg cggcagattc gtatgaacca
     5761 gcagataata ccggcggccg acagccgtgc tccactctat tacatcatcc gattccggca
     5821 ttctttgaat gtcggtgagt tccagtgcag aacccgcatc cagaggatcg gtgttggcaa
     5881 tatattcgct tcggttgtca actccatcgg catcggaatg tgccgacggc tgcgcattgg
     5941 tgcggccgcc gaagtggtcg ctctcccacc agtcgggcag gccgtcggag tctgtgtcac
     6001 cccgcaagtg cgtcaccgca agattcagcg aaccagtgga ttccagacgc caaagccatg
     6061 tgttgtccgg ggtgaacccc gcataggagc ttgatgcatt gccgagaaca atcatctgat
     6121 gaatcggata tgcacccggc gacggattca gcgaaacgcg acagaggccc gtgacggggc
     6181 ctgaagcact gagacaatcc agcgcagatt gtgatttgac atcacagtca aagcgcgatc
     6241 cactgataaa cgcgatactt ccgaaaaccg tctgagttcc aggagcacgg ccgcccggcg
     6301 caacggttcc agccacagca aggcttccct gcaccgctcc gctcccggca agcgtgctat
     6361 tggatgcgat ataaactttg gaggccgaaa tggaagcatc attgactatc atcccactgc
     6421 tcacggacat ggtttcgccg cgaacagaag aatcgatgac aacgcacaaa agaaaaacga
     6481 taccggcttt catggtaaag ccccacttcc ttgactcaag gatgccagac ccggggctct
     6541 tcgtcaaggc tgcagatctt tggggatatg cttatgaccc gaattatctc gattccagat
     6601 accatgaaaa cagatacacg gctatcgaca cggcgacata gccgaattcg acacggtgtt
     6661 ttcggggtgc gtcctcgcgt gagtgcgtga agtccagtac agcctggcgc agaagaaatg
     6721 tgtagagcac gaaatagaga aagggaatca catgcacatg gagcggatag tcggatattt
     6781 cctttttgaa cagcgaaagg atccatccaa tctgcgtgta gattccgagg aggcccagtc
     6841 cggcgttgat gaacatccac ttgacctcat caaggccgaa gatggcgaga tagaaaaccg
     6901 tatagaccag aatggcaaag ccgccatgcg catacaggaa cgccaccggc gggccgcttt
     6961 tggcataggc catggcaaaa cccgacgcgc cgaacgccag catgtgaatc agaaagaaag
     7021 gatataccga gtaccacggg atttcggaac ccttcgcaaa gtgcttgccg ttcatcgtta
     7081 tgtctccatg gagcttcatc acatcctccc ggggcagcag atattcatca gatcagaatg
     7141 acagcaccaa accggccaca gcatagtgga acagggaaaa agtgtacacc tcatcattgt
     7201 cggcgcctcc ttccagaatc cggtatccgg cctgcagcgc aagacggggg ctgacatcat
     7261 actgcagggc aaacatcaca tcctccgcac ggccctgcgg cgcggccagc gcttctccgt
     7321 ccatcagcag atgaagtttt tcgaaaggtg tccaggtgac attgaagctc agcagcggaa
     7381 ccactcccgt gttgcttttt tccgcggcca tgccgccgcc ttccagctga atggacgcat
     7441 cgcgcacttt gagcgcgccg ccgaaacaga ttttccaccg gtctgtttcg cggaagatgt
     7501 aacgataaat catacgatac gaatcaaagc ggaaggtggc ctccaccgga gatccggcgg
     7561 gaaacaggct gtcatcaaag agcgtgtccc gatccagaac ccccgccgac tccatggtca
     7621 gcggcgccgc caggaacccg acccagtgcc tgtctgaaat ggtgcagccg taacgaaccc
     7681 tgaagacaac atccccgtcc gcatcgagat cgtccgtcag cgaaaggcgg gtgccggaat
     7741 cgccggggat tgccacatcg ttgtatcccg ggaaaaaata tccggcctca aggtcgaggg
     7801 tgtgctgtgc ggatgaggcg cgggtcaacc cgatcaggca cagaaatgcg gcgataattc
     7861 tcatggtcat tgttcctcat tgttcatctc aagcgctgtt tgtaaaccgg acatttccgc
     7921 atcacggccc ttccaccaga aaagaagtgt tcccgcgatg ccaaacgcaa aggcggcttt
     7981 caggttcagt tccggcgaga tgcgccacag gagcgcgccg ccgaaggcgg cgaaggcgac
     8041 cagaacatcg cggaacaggt aataaagtcc aaacatcgcg gctttgcggc cttccggggc
     8101 aagatccata atcagcgcct tgcgcgaagg ttcgccgaac tccttgaggc cccgcaggac
     8161 aaatgccgca atcaggggcg caagcgactg tgaatgcaga agcgccagcg gaaacaacgt
     8221 gaagaacaca aaagtgatca ggacaaacgg cttctttccg aaatgatcgg ccatataggc
     8281 cacaggaaga tagatcagca cggcggccat gttttcgatg gcggaaagcc atccaaaaga
     8341 aagttccgtc accgggcgca caatgtgccg cgtggcccag atcacaacga aggcgtccgg
     8401 aatgcgctcg cagaaacgga tcaggatatc agaccacagc aggttcttca gtccgcgggg
     8461 catctcacgc cagagacgca gggggtttgg ctcggcggcc cgggcgcgct tttcgtcatc
     8521 cacaataagc atctgctgca taactgccgc aatgagcgcc aggatcagcg cggcgataaa
     8581 cgccgcgcgc accccccgct cgacccccca gatagcgata caggcgccgc ctgccacggg
     8641 gccaagcatt ttcggcacgc ggcggatcag ggccagagcg gacacaccca gcgtgcgcct
     8701 gtgcttcggc acaaccctcg ccagaaggtc catggtggcc ggaagtgaaa tggcggacca
     8761 cgagataaac agcgccgcgc ccagcaggac ggcccaccag gccgggatga agatgacgca
     8821 gagatagccg gccatggaca gcacgttaaa gaccagcagg gcgcgcttag tgcccagccg
     8881 gtcactgaga tagccgccgg ggaaggaata cagcgcggac agcaggtcgt ccatgcccgc
     8941 gagaatccct atggccaaaa tggcggttga ggcctgcgcc aattcctgca aatagagggg
     9001 cagaaagcgc tcggccatat gctcgcccat tcccaccaga acgaccatcc ccagcagtcc
     9061 cagcatgctc cgcttgtgga caccctgcgc tccagattca gaattgttta aattcataaa
     9121 cgtgcgggaa tatccatcat tcagaaagtg acgtcaacgc ggaagaccgg cacccaagat
     9181 cgggattaat ttctgggaac cggcgcgcca aatgatgcct gctcggtttc ctcgcggtcg
     9241 gcgccgcgag cggtttcata cagaaggttt cgcagatcct caaccccgcg gatgcccatg
     9301 tcccagtagt cgcggccggc gcgcgcccaa aggtagccat cgggcagcac gtttcccccg
     9361 ccggggcgga ccggatacag gttgccccag cttggattga tccattgcgg accgcgatca
     9421 ccccacacct cgagcatggc atgaccttcg ggaacggcgg agggatcgcg gcgaagcagg
     9481 ccgatccagc ctgcatcggc ggccagcaca atgcgtgcgg gcactcccgc actgcgcagg
     9541 agcgccgcgg ccaccaaggc ataatccgtg cagtccccaa acacaccgcc gcgcacaagt
     9601 tcgtcggcgg ttctcgccgc cgcatgaatg ctctcagcct gatcataggt gaactcgctg
     9661 tgaagcaggt ccatgacaat gcggattttc tgcccccggg aggaaccggc aagaagaggc
     9721 ttggccgccg cgcgcacggc cggcgtgatc cgcgtttgct ctccctcctg aagccacttc
     9781 cggttttctt cgttcagcgg aaagcttttc ggcagaaggc ccgcgctctg gcatccggca
     9841 gccgccagca gcagccccag gctgaaagcc catgcatacc gtgccttcat attgccaaga
     9901 ccgccagtgc ttcagcggtt ttcaagccag ggaggcatat ccatcttcac gcccgaggcg
     9961 ggtgtggggc cgcccggggg cggtgcgccc ttcggggcgc tctttttgat gaaacgtttc
    10021 gcttccgcct gggtcagcgc gcgctgttca acaccttttt cggtgacctg aaactggaca
    10081 aacggcgtcg tgacaacaaa cggctccaga cccgccacgc cgggtgcaac tacaagctcc
    10141 cggcgatgca gatcgaaaac cagcaggttc tcctcaccag tataggggtt gatttctatg
    10201 ccgatatcgg tgcccctgat tccaatggtc atcttcccgg tcctgagtcc gaaacgcgcg
    10261 ggattcattt caacgatttt ccccgtaatg cagcggaacg ccccctgaat catgcggacc
    10321 tgaaacgcgt tgtctttttt tcgagccggg ttgaagacaa attcattgag caccatttcg
    10381 ctgctttcgc cctgagagag ggtggattca tcaagaaacc ggatggttat ccgggaacgg
    10441 cggcgcgtca gaatacgatc gttcagaaaa acatccgcac cggaaccaag ttcttcctcc
    10501 atgccggagg ccctcacggc cacagcccca ccctccagcg ccaccacaga gccaatggcc
    10561 tgctgtccaa aaaccatctg cccggacagc agagaaaaac aaaacagcac gaccagtccc
    10621 gccagtgata tttgataacg agtcatcata agcctcccgc ctccatgtca aaaacattaa
    10681 cggtcacaac tggatcatgt caaaagccgg cgagagcatc aagcgcgatg tcgcacaaaa
    10741 gccccgccat tcagccaata tatcagcccg caacccgctg atggctgtcc cggattttgc
    10801 cggccagctc gttcagggct gcgaagctct ccgcacgggg cagtcccttg cacatcacag
    10861 cgccaagcac ttcaactttc aggttgggaa tcagaccgga aatctgttcc acggccttcg
    10921 ttccccagcc gtacgaaccg atgacggccg cgaatttcag tttcggccga atggcgttcg
    10981 caagctgcgc ggcggaataa acaaccggat gaggacccac atgaacggtt ggggtgccga
    11041 taacgatggt ggccgcatcc accagcgaca tggccagctt gccgatgtcg gtgacggaca
    11101 gatcgaactt ctgcacacca acttcccgtt caatcagcgc ctgcatcagg tggttcacca
    11161 tcatttccgt gcttccatgc attgagatgt acgggatcac aaccatgttt gagacccggt
    11221 cagaaaccca gtcccggtag gagtccagga tgctttgggg ctgatcatag atgggcccgt
    11281 gactgggggc gatcatattg aaatccagcg gctcaagttt ctggaggttc ttctggatca
    11341 cagtccggaa cggcatcatg atctctgcat aatagcgctt cgcggcttcg atgacatacg
    11401 cgctgccgcc ggcatacaga tccgacgtgg ccaggtgcga cccaaagaaa tcacaggaga
    11461 agaggatttt ttcttcgggc agataggtgg acatggtttc gggccagtgc acccacggtg
    11521 tgtagacaaa ctggagcgtc cgatccccca gtgaaagggt ttcaccatcc tccacggttt
    11581 ggacccggtc ctccggaaga gcaaggtgtt cgagcagcag cccccgggcc ttggacgaac
    11641 agagaagttt tgctccggga tattttttga gcacatccgg aagcgcaccg gagtgatcct
    11701 gctccgcgtg ctgggaaatc acatagtcga tctgcggcac atccgcaagc tgcgcaaaca
    11761 gcaggtgctg cagcgccgga tccacggcat caatcagggc ggtcttctct gaaccccgca
    11821 ccaaataggc gttatagctt gttccgtcgg gaagaggaat cagcgaatca aacaggcggc
    11881 ggttccagtc gactgcgccc acccattgaa ccctgtcaga tattgttctc ggtttcatgc
    11941 gtttgttctc cgtattcatg cgttcatgtt ttcggtccgt cggcgcgaca gcttgtgcgg
    12001 cggaagagaa acatcgtttt gaccccccgg ggcactccgc cgacttccag caaaggatag
    12061 gccaggaaat tgacgggacc ggacgcggga gccggatcga gttccaaatc ccggccgcgg
    12121 ctgaactcaa agcgattggc cgggtggtgc ccgaagtagt acatgctcag cgcgctgaat
    12181 ttgtcggctt cgctgacatc caccggccac cacttccctt ccgcatagaa ttccgcccag
    12241 ccgtgatagc cgtctgttcc gccttcatcc cgttcggacg ggatgccggc gccgattgca
    12301 aagcgggccg gaataccgac ggcgcgcgcc agaccgatga agtaggaatg gtaatccgtg
    12361 cagttgccgg acagggcatc acaggcatag acagagcttc cctgccccca gccctcgccg
    12421 aatttcatgt agcgcatttg atcaatcaca tgatcataca gagcccgggc ctgcatcagg
    12481 gcgccttcgc ggccttccac ggccttctcg gcggcggcac gaatggtttc atccgcagga
    12541 accatgcgct cgggccgcag gtgcttttcc gcttccgccg gatcgccggc ataggcggcc
    12601 ttttcaagcc gctcgacatc atatacaatt tcgatctcct tgccgctggc cgcggggccc
    12661 agttcaagaa acatcacccg gttgccatga gcttgatcgg tcagcatttt ccgggtgccg
    12721 ggcgcacgga cagatttcac ggttacattc tggaaatcgt cagactgcgc gatcggcagc
    12781 cagcagcgcg ccggaccgga aatttcaggg agccttgcct gatagcggaa ctcaaacgcg
    12841 tcgtggccgg gcagcacacc gagaagctcc cgtg
//
LOCUS       DAOQWR010000068        12341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5612_length_12341_cov_4.887270, whole genome shotgun sequence.
ACCESSION   DAOQWR010000068 DAOQWR010000000
VERSION     DAOQWR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_12341_cov_4.887270"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..264
                     /locus_tag="PKK36_06785"
     CDS             103..264
                     /locus_tag="PKK36_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNR94300.1"
                     /translation="MQKYVCTVCGYIYDPAAGDPDNGVAPGTAFEDIPDTWVCPECGV
                     PKSDFEPAD"
     gene            280..1878
                     /gene="ptsP"
                     /locus_tag="PKK36_06790"
     CDS             280..1878
                     /gene="ptsP"
                     /locus_tag="PKK36_06790"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNR94301.1"
                     /translation="MSLERRAQGLPLSGGMALGRVCLFDDHRHQEVPAYAIEPSRIDD
                     EILRLEEALQITTRQLEDIRTHVHERMGTAEAGIFSAQKMIVEDPQISREMREHIRAE
                     LSNAEHAIHRVLDAYESRLGALDDEYIRERASDVGEIRRRLLNNLANTVPGLSCAGRC
                     RRGRNRVVVAEELTPSLTISMNTDEVLAFVTERGGINSHAAILARALGIPAVSGIRNI
                     HSSVACGTDILVNGDTGEVIISPAPETLAACSCAIGRARRAEAMDPVPQLKVLANITL
                     CGEAGEACEMRAEGIGLYRTEFEFISSGEILREEEQCARYTSVVEAMNGRVTTFRMLD
                     AGGDKPLPELRIPQEENPALGLRGSRLLLRYPDLFRDQARAIARASQAGPVRVMFPMV
                     VDVQQFCRLRDAFKEYAADIRTGTVEFGVMFEVPSAAIQADEIFREAEFASVGTNDLT
                     QYLFAVDRDNALVADDYSSDHPVFWALMRTLASAARKKNRPLSVCGELAGMPDMVKKL
                     MACGIRSVSCSPRAIPLIRKAVTE"
     gene            1875..3020
                     /locus_tag="PKK36_06795"
     CDS             1875..3020
                     /locus_tag="PKK36_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR94302.1"
                     /translation="MRVGIIGAGHAGVKAAETASAQGAEVHLYSAEPVLPYYRPKLVG
                     YAFGQSSLSEITMHDAAWYESRGIALLLNTPVTSCDLSARSLTSKLGTEKYDALIFAT
                     GSLPAVPPFARHALEHIHPLWHLGHAEDIRRLLSPGKRLAVVGGGILGVEVAHRAVLA
                     GLQVVLVERLGHLMESNFGTEASVVVENSLRGMGVRLCLGTCVSSLKKAPAGDFHLQM
                     DSGDSDRADMVILAVGARRDTSLPRTAGLKCGAGILVTAGMQTSDPSVFAGGDIVEWP
                     PCPHFSVYEAVMQGKLAAANALAAAQGGAMAEHKSADVPVSYTYEGFSLNSIGPACEN
                     GADEVVLENSAEIHRALLVKAGILIGIQMAGSSVDFRKYEKMIGKKL"
     gene            3037..3609
                     /locus_tag="PKK36_06800"
     CDS             3037..3609
                     /locus_tag="PKK36_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNR94303.1"
                     /translation="MELKGSQTEKNLLTAFAGESQARNRYTYYAGAAKKEGFRQISEI
                     FEETANQEKEHAKRLYKFLKGGELEITASFPAGTIASTSVNLEHGAAGEHYEWTEMYP
                     SFAETARKEGFPEIAKVFEAIAVAEKQHEKRYLDLKANVDAQKVFKKDKEVVWRCMNC
                     GYIHTGKEAVTVCPACAHPQAHFELLAENW"
     gene            3633..4010
                     /locus_tag="PKK36_06805"
     CDS             3633..4010
                     /locus_tag="PKK36_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNR94304.1"
                     /translation="MTKRLEIYKCEMCGNIVSVLHAGDGELVCCGEPMKRLEEKTADK
                     TTEKHVPVIEKTDTGYKVTVGSTLHPMLPEHYIEWIEIQFDDKVGRKFLKPGDQPVAV
                     FDGISAKDVSAREYCNVHGLWKS"
     gene            4086..4604
                     /locus_tag="PKK36_06810"
     CDS             4086..4604
                     /locus_tag="PKK36_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNR94305.1"
                     /translation="MISEKLAKAINDQINKEIYSSYLYYAMAAQAEAQGFKGCANWFH
                     IQALEELTHADKFFKYLCSQGALPVMEAVDKPELKGTEIKALFEQTLEHERFVTSRIN
                     SLMKLTREEGDYASEIFLQWYVTEQIEEEENATGILDQIRLTGDSKQGMFMLDRELAA
                     RVFTPPPAPAAD"
     gene            4608..5087
                     /locus_tag="PKK36_06815"
     CDS             4608..5087
                     /locus_tag="PKK36_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNR94306.1"
                     /translation="MPVLIAASVLGALIGFIAGLKVRKLRLAPVNAAFIGAVIGGFIA
                     WHEVGQRTPENIYMLQRGEALSGIVQSNRAVVVDYYADWCPPCRSLKPVLDDLAGVWS
                     ERVLFVGVNVDHHPAEAQAAEVQSIPDVRLFLNGKLLRKISGFNTRSVYDTALKDLL"
     gene            5138..6151
                     /locus_tag="PKK36_06820"
     CDS             5138..6151
                     /locus_tag="PKK36_06820"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HNR94307.1"
                     /translation="MNAVLNILNSFWRVLVEMSPYLLFGFFVAGILSMFISASTVERH
                     LGGRGIWPIIKASAFGVPLPLCSCSVIPVSASLRKHGAGKGATTAFLISTPQTGVDSI
                     FVTYSLLGLVFAIVRPIAALLTGILGGWLVDVTAGAGPDQAQEPCHDACCVHEEKRHR
                     IRDGLRHGFITIPKHIARDMVIGLALAALITALIPDDFFAEYLGTGFLSMVVMMLMGI
                     PVYVCATASVPVAAAFMMKGITPGAAFVFLMTGPATNAATIAIIRNMMGNKSALIYLA
                     TVAVSALAFGLLLDHAIMSGHVHIHDMAHAMLPYWIKLIASAVLAVVLGYALWSNFHP
                     GRK"
     gene            complement(6225..6920)
                     /gene="queC"
                     /locus_tag="PKK36_06825"
     CDS             complement(6225..6920)
                     /gene="queC"
                     /locus_tag="PKK36_06825"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNR94308.1"
                     /translation="MKTDSAKPVAVVAMSGGLDSCVTAAIAAQDYEPALLHVNYGQRT
                     EARELKAFNDIADFYGVKRRLVISLDALKAIGHSSLTDFQMPVSEANLESTEVPASYV
                     PFRNAHMLATAASWGEVLGAKAIFIGAVEEDSSGYPDCRAAFYRAFEAAIEEGTKPET
                     HMRIITPVITLNKAAIVKEGLRLGAPLHLSWSCYQSEDKACGVCDSCALRLRGFRQAG
                     VEDPIPYAIRPNY"
     gene            complement(6917..7339)
                     /locus_tag="PKK36_06830"
     CDS             complement(6917..7339)
                     /locus_tag="PKK36_06830"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNR94309.1"
                     /translation="MKISREFNWEMAHRLREHSGGCQNVHGHSYRMTVDICGEPDPAT
                     GMVLDFADLGEMLKPLIEELDHAFLCEARDDSLIEFLKKEKMKHKIVPFRTTVENLCG
                     HVARALKPGLEACSRLSEFSVTIHETARNSGTVTVKLK"
     gene            complement(7345..7995)
                     /locus_tag="PKK36_06835"
     CDS             complement(7345..7995)
                     /locus_tag="PKK36_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR94310.1"
                     /translation="MSVEILKIAEIFYSLQGESTFAGLPCVFVRLSGCNLQCTWCDTA
                     YANDHPAEMSVDDIMEHVSRWPCRLVEVTGGEPLLQPAVHQLIAGLCSQGYEVLLETS
                     GAEDISGVDPRVHRIMDIKCPGSGMGAHNRGENLSHLTANDQIKMIIAGRTDYEWARD
                     LIREKQLDRICPVLMAPAFGRVSNTDLAQWILEDHLPVRYQLQLHKYIWPADWKGV"
     gene            8045..8593
                     /locus_tag="PKK36_06840"
     CDS             8045..8593
                     /locus_tag="PKK36_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNR94311.1"
                     /translation="MTLSRKKAWTVLYWNVVFAATVVCLGLTLSLRQMHAYDMLIVKI
                     GREHNVDPRLISAIIWKESRFDPVRTGQAGEIGLMQVREGAYREWEEAVGERHARHEL
                     FNPEINIRAGTWYFARAIRTWSIYSDPIPYALAHYNAGYSNAKRWAAVPHGTSREFWN
                     NITYPGTQRYVRDILARYRGGI"
     gene            complement(8598..10340)
                     /locus_tag="PKK36_06845"
     CDS             complement(8598..10340)
                     /locus_tag="PKK36_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94312.1"
                     /translation="MVSNTPPAEWQTYRRLLHYARPYSVRIALGLFFGMIFGGSYLGI
                     LLSLKKNLAAMFDTSTLPLGIILSIAALLPVCTVFMGIGNFFSKYFVEWVGNRVVMDL
                     RTQTFAHIHDLSLLYFTRSRTGELISRTANDSALVERAVSTVLGDLVREPFVLASAVG
                     YLLWLDWRLCLVSLVVFPLCIIPVAIFGRRVRKASREGQQKLADIVTILQETITGVRV
                     VKAFGMEDYEKARFNSRSRSVFSRLMRVTKAKAAVEPVIVVISSIGLSAVLVYARIVH
                     MPMEEFFTFAAALMMMYQPVKNLSKLHLNIQHSSAAADRIFEVLDTEISVVEAPDAVP
                     FQERIETVRFDHVSFSYEEASPVLSDISFSVKAGECVAFVGSSGSGKTTLVGLLPRFF
                     DPTGGQILFNSEPAQKFTLASLRAQMGIVTQETILFNDTVANNIAYGDRNASREAIEQ
                     AARKAHAHDFIMEMPEGYETMIGERGVRLSGGQCQRLAIARAMLRNPPVLILDEATSA
                     LDTESERQVQAALDELMTSRTVFAIAHRLSTIAHADRIIVLDKGRIAEQGTHEDLIAR
                     DGLYKYLYDLQFSR"
     gene            complement(10330..11256)
                     /gene="trxB"
                     /locus_tag="PKK36_06850"
     CDS             complement(10330..11256)
                     /gene="trxB"
                     /locus_tag="PKK36_06850"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNR94313.1"
                     /translation="MTRNMIILGTGPAGLTAAIYAARARLEPLVVEGMQPGGQLTSTT
                     DIENYPGFENGIGGFELMDIMKRQAVRFGTDFVSGTAVSVDFKNRPLRITLSDGSVHE
                     TETLIIATGASARYLGLESEQKLMGKGVSGCATCDGAFFRDVPVAVIGGGDTATEEAL
                     FLTRFASKVYIIHRRDQLRASRIMADRVLSHAKIEMVWNSVVEDVLDVAQDKVTALRL
                     KNVKTGASSELPVEGMFLGIGHQPNTEPFKGHLKLNEQGYIITQETSTDIEGVYAAGD
                     VQDPHYRQAVTAAGSGCMAALKAERYLEEHGF"
     gene            11385..12098
                     /locus_tag="PKK36_06855"
     CDS             11385..12098
                     /locus_tag="PKK36_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94314.1"
                     /translation="MGLFDLSKASLQEWRVSYRRTQAMGAVPLLFALFTIAFVPGRSG
                     PVIWGALGFAAGLHLILWGGYHLHLAHFPDSSRVIPQSRFTPFLLFTIFHLFGWTILV
                     LLWNSLIRLGYSFTPLMNLVFFLLLPVIPAKACWLHLAEQSHEKRHAIVNEVLRYALL
                     IGIVLLAALAGTHAVMAGDGALAEKIPAKIIWIWVAAALLILTCLILLAEHLHAFLMS
                     RTPVRENARPKTDEPKLPY"
BASE COUNT         2590 a         3367 c         3681 g         2703 t
ORIGIN      
        1 cgtcggcggc ttcagacgaa ggataagctt gcactctccg gccgcgcggc tactatggca
       61 gagacaggtc ttggatgaac aaaccaaaca ggagtatgtg aaatgcagaa gtatgtttgc
      121 accgtgtgcg gctacattta tgacccggcg gccggagatc ccgataacgg ggttgctccc
      181 ggcacagcgt ttgaagacat tcccgacacc tgggtctgtc ctgaatgcgg cgttcccaag
      241 agcgattttg agccggccga ctgaacggga tgtgaaataa tgagcctgga gagacgcgct
      301 caggggcttc cgctaagcgg aggaatggcg cttgggcgcg tttgtctatt tgatgatcat
      361 cgtcatcagg aagttccggc ctacgccatc gaaccctccc ggatcgacga tgaaattctc
      421 cgcctggagg aagcgctgca gatcacaacc cggcagctcg aggatatccg cacccatgtt
      481 catgagcgca tgggtacggc ggaagccggt atattcagcg ctcagaaaat gattgtcgag
      541 gatccgcaga tatcaaggga gatgcgggag catatccgtg cggaactgag caacgcggaa
      601 catgccatcc atcgtgtgct ggatgcatat gaatccaggc tgggcgcact ggatgacgag
      661 tacatccgcg agcgggcatc ggatgtcggt gaaatccgcc ggcggctgct caacaatctg
      721 gctaatacag ttcccggcct gagctgtgcc ggccgctgcc ggcggggcag aaaccgcgtt
      781 gtggtcgccg aggaactgac gcccagcctg accatcagca tgaacacgga tgaagtgctg
      841 gccttcgtga ccgagcgcgg cgggatcaat tcgcacgcgg ccattctggc gcgtgctctc
      901 ggcattcccg cggtcagcgg aatccggaac atacacagct cggtcgcctg cggaaccgac
      961 atactggtta acggagacac cggcgaggtg atcatatccc ccgcgccgga gactctggcg
     1021 gcctgttcct gcgccatcgg ccgtgcccgc cgggcggaag caatggatcc tgtcccgcag
     1081 ctgaaggttc tggcgaatat cacgctgtgc ggggaagcgg gcgaggcctg tgaaatgagg
     1141 gccgagggga tcgggctcta ccggacggaa ttcgagttta tttcttccgg agagatcctg
     1201 cgggaagagg agcagtgcgc ccggtacacg tccgtggtcg aagccatgaa cgggcgtgtc
     1261 accacgtttc ggatgcttga tgccggaggg gacaagcctc tgccggagct gagaatcccg
     1321 caggaggaaa atcctgcact ggggctgcgg ggaagccgcc tgctgctgcg ctatccggac
     1381 ctgtttcgcg atcaggcgcg cgccattgcg cgggcatcac aggccggccc ggtccgcgtg
     1441 atgtttccca tggtcgtgga tgtccagcag ttctgcaggc tgcgcgacgc ttttaaggag
     1501 tatgcggctg atatccgcac gggaactgtt gaattcggcg tgatgtttga agtcccttcc
     1561 gcagccattc aggccgatga aatattccgc gaagccgagt tcgcaagcgt ggggacgaat
     1621 gatctcaccc agtatctttt cgcagtggat cgtgacaacg cgctggttgc ggatgattac
     1681 agctcggatc atcccgtgtt ctgggcactg atgagaaccc tggcctccgc ggcgcgcaaa
     1741 aagaacagac ctctgtctgt ctgcggcgaa ctggccggca tgccggacat ggtcaaaaag
     1801 ctgatggcct gcggcatccg ctcggtgagc tgcagtcccc gcgccattcc cctgattcga
     1861 aaggcggtga ctgaatgaga gtgggcatta taggcgcggg ccatgccgga gtgaaagctg
     1921 cagaaacggc ttccgcgcag ggggcagaag ttcatctgta ttccgcggaa ccggttttgc
     1981 cctattaccg gcccaagctg gtgggctatg cctttggtca gtcgtctctt tcagagatca
     2041 ccatgcatga tgcggcgtgg tatgaatcgc gcgggatagc gcttctcctg aatacacccg
     2101 tcacaagctg tgatctctcc gcgcgcagcc tgacatccaa actcggaacg gaaaaatacg
     2161 atgcactgat attcgcaaca gggtctcttc ccgccgtgcc gccttttgcc cgtcatgcgc
     2221 tggagcacat ccatccgctc tggcatctgg gccatgccga agatatacgg cgcctgcttt
     2281 ctcccgggaa gagactggcg gtcgtaggcg ggggcattct cggggtggag gtggcccatc
     2341 gcgccgttct ggcgggactg caggttgttc tcgtggagcg gctgggacat ctgatggaat
     2401 ccaattttgg aacggaggct tcggtcgttg ttgaaaattc cctcaggggc atgggggtca
     2461 ggctttgtct gggcacatgc gtgagctcgc tgaagaaagc gcctgccggt gatttccact
     2521 tgcagatgga ctccggggac tcggatcggg ccgacatggt tatccttgcg gtgggcgcgc
     2581 ggcgggacac ttccctgccg cgcacggccg gtctcaaatg cggcgccggc attctcgtga
     2641 cggccggcat gcagacttcg gatccgtcgg tttttgccgg gggcgacatt gtggaatggc
     2701 cgccctgtcc gcatttttct gtttatgagg cggtcatgca gggcaaactg gcggcggcca
     2761 acgcgttggc tgccgcgcag ggcggagcga tggctgagca taaatcggca gatgttcccg
     2821 tatcctatac gtatgagggg ttctccttaa attccatcgg tccggcttgc gaaaacggcg
     2881 cagatgaggt agttttagag aatagtgctg aaattcacag agctcttctt gtgaaagcgg
     2941 ggatactgat tggaatccag atggcgggat cctccgtgga tttcaggaaa tatgaaaaaa
     3001 tgataggaaa aaaactgtaa tcaggagaca ggaaaaatgg aattgaaagg cagtcaaacc
     3061 gagaaaaacc ttttgaccgc gtttgcgggg gaatcccagg cccgcaaccg ctacacctac
     3121 tatgccgggg cggcgaagaa agaaggtttc cgtcagattt cggagatttt cgaggaaaca
     3181 gcaaatcagg aaaaagagca tgccaagcgc ctctacaagt ttctgaaggg cggcgaactg
     3241 gaaatcacgg cctctttccc ggccggcacc attgcatcca cttctgtaaa tcttgagcat
     3301 ggcgccgcgg gagaacacta cgagtggact gaaatgtatc cctcgtttgc ggaaaccgcg
     3361 cgcaaagaag gattccccga gatagcgaaa gtctttgaag ccatcgcggt tgcggaaaag
     3421 cagcacgaaa aacgttatct cgacctgaag gctaatgtgg atgcccagaa ggtgtttaag
     3481 aaggacaagg aagtggtctg gcgctgcatg aactgcggct atattcacac cggcaaagag
     3541 gcggtgaccg tgtgcccggc ctgcgctcat cctcaggctc actttgaact gctggcggaa
     3601 aactggtaat cgaacaacat aaggagaagc ccatgacaaa gcgtcttgaa atctacaaat
     3661 gtgaaatgtg cggaaacatc gtcagtgttc tgcatgccgg agacggcgaa ctcgtgtgct
     3721 gcggcgagcc gatgaagcgg ctcgaagaga agaccgccga caagaccacg gaaaaacatg
     3781 ttcccgtgat cgagaaaaca gataccggat acaaggtcac cgtgggaagc acactgcacc
     3841 cgatgctgcc ggagcattac attgagtgga ttgagattca gtttgacgac aaggtgggcc
     3901 gcaaattcct gaagcccggc gaccagcctg tggccgtgtt tgacggaatc agcgccaaag
     3961 acgtgtccgc gcgcgagtac tgcaacgtac acggtctctg gaagtcctga ggtccgtatg
     4021 atcacagaaa gccgggggcc gtcacaggcc cccggcttca tctctaatca gcagcaggag
     4081 aaaaaatgat atccgaaaaa ctggcaaaag cgattaacga tcagatcaac aaggaaatat
     4141 attccagcta tctgtattac gcgatggcgg ctcaggccga ggcgcagggt ttcaagggat
     4201 gtgcgaactg gttccacatc caggccctgg aggaactgac tcatgccgac aagttcttta
     4261 aatatctgtg cagtcagggt gctctgcctg tgatggaggc cgtggacaag cccgaattga
     4321 aggggacaga gatcaaggcc cttttcgagc agacgctgga gcacgagcgt tttgtgactt
     4381 cccgtattaa cagcctgatg aagcttactc gtgaagaggg cgactatgcc tcggagatat
     4441 tcctgcagtg gtatgtgacc gagcagatag aggaagagga aaacgccacg ggcatcctgg
     4501 atcaaatccg cctcacgggt gattccaaac agggcatgtt catgctggat cgagaactgg
     4561 ccgcgcgcgt gttcactccc ccgccggccc ccgccgcgga ctgattcatg ccggtattga
     4621 ttgcggcttc ggtgctgggt gctctgatcg gattcattgc gggcctgaag gtgcgaaaac
     4681 tccggctggc tcctgtgaac gcggccttta tcggtgccgt gatcggaggt ttcattgcat
     4741 ggcatgaagt cggacaaagg actccggaga acatctacat gctgcagcgc ggtgaggctc
     4801 tcagcggcat tgtgcagagc aaccgtgccg tggttgtgga ttattatgcc gactggtgtc
     4861 ctccctgccg aagcttaaag ccggttcttg atgatctcgc cggtgtgtgg tcggaacgcg
     4921 tgctgtttgt cggggtgaat gtggatcatc atccggctga agcacaggcg gctgaagtgc
     4981 agagcatccc ggatgtccgt ctttttctga acgggaaact gctccggaaa atcagcggtt
     5041 tcaatacgcg ttcggtttac gacacggcgc tcaaagatct gctctgattt gcgggatcgg
     5101 acgttctgct gtataatgta tggaatatca gggaataatg aatgctgtgc tgaacattct
     5161 gaattcgttc tggagggtgc ttgtggagat gtccccgtac cttctgttcg gattttttgt
     5221 cgcgggcatc ctgtcgatgt tcatctcggc ttcaacggtg gagcggcatc tgggcggccg
     5281 cggcatatgg ccgattatca aggcctctgc ttttggcgtt ccgctcccgc tttgcagctg
     5341 cagtgtgatt cccgtatccg cgtcgctccg aaaacacggc gccgggaagg gggccaccac
     5401 ggcttttctg atttccactc cccagaccgg cgtggacagc atctttgtga cttacagcct
     5461 gctgggtctt gtctttgcca ttgttcgccc cattgccgcg ctgctgacgg gaatcctggg
     5521 aggatggctt gttgatgtca cggcgggggc gggacctgat caggcgcagg aaccctgtca
     5581 tgatgcctgc tgtgttcatg aggaaaaacg ccatcgcatc cgggacggcc tccgtcatgg
     5641 ttttatcacc attccgaaac acattgcgcg cgacatggtg attggccttg cgctcgcggc
     5701 cctgattacg gcgcttatcc ccgatgattt cttcgcggaa tatctgggta ccggattctt
     5761 atccatggtt gtgatgatgc tgatgggcat tcccgtttat gtctgtgcga ccgcttccgt
     5821 tcccgtggcg gcggctttca tgatgaaggg cattacgccc ggcgcggcgt ttgtgtttct
     5881 gatgaccggt ccggccacca acgcggcgac cattgccatc atccgcaata tgatgggaaa
     5941 caaatccgca ctgatttatc tggcgaccgt ggcggtttcg gcgctcgcct tcggtctgct
     6001 gctggatcat gccatcatgt cgggtcatgt ccacattcat gacatggccc atgcaatgtt
     6061 gccgtactgg atcaagctga ttgcgtcagc tgtgcttgcg gttgtgctgg gatatgccct
     6121 gtggtccaat ttccatccgg gaaggaaata aacatgtgtc tgaacaagat aaagccgggc
     6181 cgccctctga cttgcgcccc ctcctgtatg ctttctggcg gatttcaata attcggccgg
     6241 atggcgtagg ggatggggtc ctcgacgccg gcctgccgaa agccgcgcag gcgcagcgcg
     6301 cagctgtcgc agacgccgca ggccttgtcc tcgctctggt agcatgacca cgacagatgc
     6361 agcggggccc ccagcctgag gccctctttc acgatagcgg ccttgttcag ggtgatgacc
     6421 ggagtaatga tgcgcatgtg cgtttcgggc tttgtgcctt cctcgatcgc ggcctcgaaa
     6481 gcgcggtaga atgctgcccg gcagtcggga tagccggagg aatcctcctc aaccgcaccg
     6541 ataaaaatgg cttttgcccc cagcacttcc ccccagcttg cggcagtggc cagcatgtgt
     6601 gcattgcgga aggggacata ggaggccgga acttcggtgc tttccagatt ggcttcggac
     6661 accggcatct ggaaatcggt caggctcgaa tgcccgatgg ctttcagagc atccagcgag
     6721 atgaccagcc gccgttttac gccgtagaaa tcggcgatgt cgttgaaggc cttgagttcg
     6781 cgtgcctcgg tgcgctggcc gtagttcaca tggagcaggg ccggctcgta atcctgggcc
     6841 gctatggcgg cggtcacgca gctgtcgagc cctccgctca tcgccacgac ggcaacgggt
     6901 tttgcggaat ctgttttcat ttgagtttca cggtgacggt ccccgagttt cgtgcggtct
     6961 cgtggatggt tacggaaaat tcagacagcc ggctgcaggc ttccagcccc ggcttcaggg
     7021 ccctggccac atgtccgcag agattttcga cggtggtgcg gaacggaacg attttgtgct
     7081 tcatcttctc ttttttcagg aattcaatca gggaatcgtc acgcgcctcg cacaggaatg
     7141 cgtggtccag ctcctcaatc agcggcttga gcatttcgcc cagatccgcg aaatcgagca
     7201 ccataccggt cgcaggatcg ggctcaccgc agatatccac ggtcatgcgg taggaatggc
     7261 catgcacgtt ctggcatccg ccggaatgct cccgcaggcg gtgcgccatt tcccagttga
     7321 attctctgct gatcttcatg cggttcaaac ccctttccag tcggccggcc agatgtattt
     7381 gtgaagctgc aattgatagc ggactggaag atgatcttca agaatccact gtgcaagatc
     7441 cgtattcgac acccgtccga acgccggggc catcagcacg ggacagatgc ggtcgagctg
     7501 cttttcccgg ataaggtcgc gggcccactc atagtcggtc cgaccggcaa tgatcatctt
     7561 gatctgatcg tttgcggtca gatggctcag gttctccccg cggttgtgcg cgcccattcc
     7621 ggaaccggga catttgatat ccatgatgcg gtgcacgcgc ggatccacgc cggatatgtc
     7681 ttcggctccg gatgtttcca gaaggacctc gtacccctga ctgcagagtc cggctatcag
     7741 ctggtggacg gccggctgca gcaggggttc tccgcccgta acctcaacca gccggcaggg
     7801 ccagcgggag acatgctcca taatgtcgtc cacggacatt tccgccggat gatcattggc
     7861 ataggcggtg tcgcaccatg tgcactgaag attgcagccg gaaagacgca caaagacaca
     7921 gggaagtccg gcaaaggtgg actcgccctg caggctgtag aaaatctccg ctattttcag
     7981 tatctctacc gacataatca ccatccggct ggacaacacc gccaaaagcc tttaggtttc
     8041 gggcatgacg ctgtcgcgca agaaagcatg gaccgtactc tattggaatg tggtgttcgc
     8101 cgcaacagta gtctgccttg gtttgacact ttccctgcgg cagatgcatg cctatgacat
     8161 gctcattgta aagatcggcc gcgagcacaa tgtggatcca cggctgattt cggccattat
     8221 ctggaaggaa agccgctttg atcctgtgcg aaccggccag gccggcgaga tcggtctgat
     8281 gcaggtgcgg gagggtgcat accgcgaatg ggaggaggcg gttggggaac ggcatgcgcg
     8341 gcacgagctc ttcaatcctg aaatcaacat tcgggcgggc acctggtatt ttgcccgcgc
     8401 gattagaacc tggtccatat acagcgatcc gattccctat gcgctggcgc actataatgc
     8461 gggatattcc aacgcgaagc ggtgggcggc tgtcccgcac gggacttccc gcgaattctg
     8521 gaacaacatc acctatccgg gcacacagcg ctatgtgcgg gatattctgg cccgctatcg
     8581 cgggggcatc tgacccgcta gcgggaaaac tgaaggtcat agaggtattt gtacagcccg
     8641 tcccgggcaa tgagatcctc gtgtgtgccc tgttccgcaa tgcgcccttt gtccagcacg
     8701 atgatccggt cggcatgcgc gatggtcgag aggcggtgtg caatggcaaa gaccgtgcgg
     8761 ctggtcataa gctcatcgag cgcagcctga acctgccgct cggactccgt gtccagtgcg
     8821 ctggtggctt cgtccaggat cagaaccggc ggattgcgca gcatggcgcg cgcaatggca
     8881 agccgctgac attggccccc cgacaggcga accccccgct cgccgatcat cgtttcatat
     8941 ccttccggca tttccatgat gaagtcatgc gcatgggctt ttcgggcggc ctgctcaatg
     9001 gcctcgcggg acgcgttccg gtccccataa gcaatgttgt tggccaccgt gtcgttgaaa
     9061 agaatcgtct cctgtgtcac aatacccatt tgagcgcgaa gcgaggccag cgtgaacttc
     9121 tgcgccggct cgctgttgaa aagaatctgt cctccggtgg gatcaaagaa acgggggagc
     9181 aggcccacca gagtggtttt acccgaaccg gaacttccga caaaagccac acattcgccg
     9241 gctttgacgg agaaactgat atccgagagg accggactgg cctcttcata gctgaaagac
     9301 acatgatcaa aacgcacggt ttcaatgcgc tcctgaaaag gaaccgcatc aggcgcctcg
     9361 accacggaga tttcagtgtc cagcacttcg aaaatgcggt cggcggcggc gctgctgtgc
     9421 tgaatattca ggtggagttt gctgagattt ttcaccggct ggtacatcat catcagcgct
     9481 gcggcaaatg tgaagaactc ctccatgggc atatgcacga tgcgggcata aacaagcacg
     9541 gcggaaagtc cgattgagga tatcaccaca atcacgggct ccacggcggc tttggccttg
     9601 gtgacgcgca tgaggcggga aaacacggaa cgactgcggc tgttgaagcg ggccttctcg
     9661 taatcctcca tgccgaaggc tttgaccacc cgaaccccgg tgatggtctc ctggagaatg
     9721 gtgacgatgt ccgccagctt ctgctgccct tcgcgcgagg ccttgcgcac ccgccggccg
     9781 aatatggcca cgggaatgat gcacagagga aagaccacaa ggctgacaag acaaagtctc
     9841 cagtccagcc agagcagata tcctacagca gatgccagga caaacggttc acgcaccaga
     9901 tcgccgagca ctgtggaaac ggcgcgttca acaagggcac tgtcattggc tgtgcgcgaa
     9961 atcagttcgc ctgtgcggct gcgcgtaaaa tagagcaggc tgaggtcatg aatgtgagcg
    10021 aaagtttgtg tgcgcaggtc catcaccacg cggttcccga cccattccac aaaatatttg
    10081 ctgaaaaaat tgccgattcc cataaagaca gtgcagaccg gaagcagggc ggcaatggag
    10141 agaatgatcc ccagcggcag cgtggacgta tcgaacatgg cggcgaggtt ttttttaagc
    10201 gaaaggagaa tgccgagata cgagccgccg aatatcatgc cgaaaaaaag acccagggca
    10261 atgcggacgg agtagggcct cgcataatgc agcagccggc gataggtttg ccactcggcg
    10321 ggtggagtgt tagaaaccat gttcctccag atagcgctca gccttcaggg ccgccatgca
    10381 gccggagccg gctgccgtga ccgcctgccg gtagtgcggg tcttgaacat cgcctgcggc
    10441 atagacccct tcgatgtccg tggaggtttc ctgcgtgata atgtaaccct gctcgttcag
    10501 tttgagatgg cctttgaacg gttctgtgtt gggctgatgg ccaattccaa gaaacatccc
    10561 ttcgaccggc agttcggacg aggcgcctgt tttgacattt ttcaggcgca gggccgtgac
    10621 cttgtcctgg gccacatcca ggacatcctc gaccacactg ttccaaacca tctcgatttt
    10681 cgcgtgcgaa agcacgcgat cggccatgat tctggatgcg cggagctggt cgcggcggtg
    10741 aataatatag accttcgagg cgaaacgagt caggaagagc gcttcttctg tcgcggtgtc
    10801 ccctccgccg atcaccgcca cgggaacatc gcgaaaaaac gccccgtcgc aggtggcgca
    10861 tccggacacc cctttgccca tgagtttctg ttctgattca aggcccagat agcgcgcact
    10921 ggcgcccgtg gcaatgataa gcgtctctgt ttcatgcaca gacccgtcag acaacgtgat
    10981 gcgcaggggt cggttcttga aatccacaga cacggcggtt cctgaaacga agtcagtgcc
    11041 gaaccgcaca gcctgccgtt tcatgatatc catcagctca aatccgccaa tgccgttttc
    11101 aaaacccgga taattctcaa tgtcggtggt gctggtgagc tggccgccgg gctgcatgcc
    11161 ttccacaacc agcggctcca gcctggcgcg cgccgcatag atggcggcgg tcagtcccgc
    11221 cggacctgtg cccagaatga tcatatttct tgtcatgcca gaaactccga aagatttgtt
    11281 ttcaggatta ttagcttatc gcatgaggca tgacaagcag aaggaagact ttccccttgg
    11341 ggggtgatgt gataggcttc cgctgactga atggagaaag tcgaatggga ctgtttgatt
    11401 tgtccaaagc gtctttgcag gagtggcggg tatcttaccg ccggacccag gccatggggg
    11461 cggtgccgct tctgtttgcc cttttcacca ttgctttcgt gcctggccga agcggcccgg
    11521 tgatctgggg ggcactgggg tttgctgcgg gtctgcattt gatattgtgg ggcggatatc
    11581 acctccatct ggcacatttc cccgacagtt cccgcgtcat accgcaatcc cgcttcaccc
    11641 cgtttctcct ttttaccatt tttcatctgt ttggatggac catcctcgtt ctgctctgga
    11701 acagcctgat ccggctcgga tattctttta cgccgcttat gaatctggtt ttcttccttc
    11761 tgctgcccgt cattcctgca aaggcctgtt ggctccatct ggccgaacaa tcgcatgaaa
    11821 aacgccacgc cattgtaaat gaggtgctgc gttatgccct gctgatcggg attgttctgc
    11881 tggcggctct tgcgggcact catgcggtca tggcgggcga tggggccctt gcggaaaaaa
    11941 ttccagccaa gattatttgg atttgggtgg cggcagcact gctgatcctc acctgtctaa
    12001 tcctgctggc ggagcatttg cacgcctttc tgatgtccag gacgcccgtg cgtgaaaatg
    12061 cgcgcccaaa gacggacgag ccaaagctcc catattagtc tgcaagtctg cgcttgattc
    12121 gatgtcctgt ttttttgtct aatcccgttc tatgaaagaa gaatattttc cgcagaatga
    12181 atatcacacg ccgccggata tgccgcgcaa actgggtgac cgcctgctga tgggttcgcg
    12241 agtgtatttt atgtcacgtt ttctcggata tcttttcaag tcacggcgga ttgccctgga
    12301 aggaaaatat gaccgggctg cgtgggttca tgcttcgtat g
//
LOCUS       DAOQWR010000069        12279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5643_length_12279_cov_5.194781, whole genome shotgun sequence.
ACCESSION   DAOQWR010000069 DAOQWR010000000
VERSION     DAOQWR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_12279_cov_5.194781"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PKK36_06860"
     CDS             <1..1030
                     /locus_tag="PKK36_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94315.1"
                     /translation="DWWHKYLEVLTNGTIVQFAIMMKDAYGTEFWDNNGGSDYVVTIG
                     SVEPPPQGGDKPYSTNPSLGQRGTMTIDGNPSGWTTNHLIALDMANDDPRSLSSNWTM
                     HEAPIDLTHMWAAWDDDNLYLAWQYVDVTDVIDPANAGGAASGKISNNDGILQWIVID
                     TKASEGATTDVWGKKNSWVGANKPDYQIYLAGSLWQGYISRAVDGVFALDDGGTNYNT
                     IAAAGITAAKGSTFGAASLWGVGDADNRFDGGAPTRNFLSEGHSTSRDSFYEMSIPLS
                     YLEITAAQLDSGAGIGIMIGGGSESSMDSIPHDETTLDLPGVESYNSSFEWGDSDNFT
                     SPFSRIGH"
     gene            1237..3045
                     /locus_tag="PKK36_06865"
     CDS             1237..3045
                     /locus_tag="PKK36_06865"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNR94316.1"
                     /translation="MTAPTKNKKLLAWVAEVEKMCTPDRVVWCEGTKSEYDRLMNQMV
                     ESGSAVRLNEKLRPNSFAFNSDPSDVARVEDRTYIASRTKEEAGPTNNWIDPVELKKT
                     MRALYTGCMKGRTMYVIPFSMGPIGSPIAKIGIEITDSPYVVCNMHIMTRVSKKVLQV
                     LGDDGEFIPCLHSVGAPLEPGQKDVPWPCAPIEKKYIAHFPEKNLIWSFGSGYGGNAL
                     LGKKCLALRIASNMARNEGWMAEHMLILRMINPEGRKYHIAAAFPSACGKTNLAMLQS
                     TIPGWKVETIGDDIAWMKIRPDGRLHAINPEYGFFGVAPGTSKASNPMALASCSKNSI
                     FTNCVVTSEGDIWWEDIGCECRSGTDWKGQPFHSGMKDKDGRAVKGAHPNARFTAPAS
                     QCPVICPDWEDPEGVPIDIFIFGGRRTTTVPLVHEALSWEHGVFMGATAASEPTAAAL
                     DVKQHLRFDPFAMKPFCGYNAADYFAHWFKMGARMDDKAPRIFYVNWFRKNKDGKWLW
                     PGFGENSRVLKWMCERVDGRVEARETPVGLMPKDGDLDLKGLNISPDDYAELMKVDAG
                     LYRNMLSDIADYLKEFGDRLPSQMTHQLEALRKRLD"
     gene            complement(3089..3556)
                     /locus_tag="PKK36_06870"
                     /pseudo
     CDS             complement(3089..3556)
                     /locus_tag="PKK36_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
     gene            3658..5067
                     /locus_tag="PKK36_06875"
     CDS             3658..5067
                     /locus_tag="PKK36_06875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR94317.1"
                     /translation="MKQFWTEPAPADVEAARRRTLKLSVWDGAFWSVMAGFGDMYLSP
                     FAVFLRAGSWAIGLLTTMPPLLGALAQMIGAHWADRLGRRRDLIVPLIMLHALVFLPI
                     FFVPLLFRSAAVPALILLAALGVMFNMAAAPIWTSMMGEVVPGATRGDYWGRRSRILT
                     LTVLLASLTAGGILSLFQRAGLTWAGFGILFSVAFLARFISALILRRHYDPPYAPGPE
                     AHFTFLDFIRRMPHSNFARYALYFSLMMGASNIAAPFFTVYMLRDLKWDYAHYTLSQA
                     VFLLTQVLLVGWWGRIGDRYGNRVVIAATSFILPLLPFLWMFSTNYSYLLVVQLVSGI
                     GWSGFTIAAQNFTFDAVSVQKRARVAACVSVLHGLCMFAGGTLIGAPLANHLPSVYTL
                     GPLRVGFISSLPGLFAVSGLARFAVAALLLRRFAEVRDGHEQIHPAQLILRLAGGGII
                     AGFLNGLLRPEGKEGPSAG"
     gene            complement(5030..5683)
                     /locus_tag="PKK36_06880"
     CDS             complement(5030..5683)
                     /locus_tag="PKK36_06880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR94318.1"
                     /translation="MKIRAVFFDLDGTLLDTLQDLADSANAALARNGLPVHPIKAYRH
                     FVGSGAITLITRAIPPERRTEDTVQACYKDFRGEYDARWDHKTRLYDGIPQLLDALHQ
                     NGVRMAVLSNKPQAAVELCVKRFLSRWTFDPVRGEQPGVPVKPDPAGALAMTRHLGLR
                     PEEVLYLGDSDVDMQTAVRAGFFPAGALWGFRTEDELRQAGARALLSRPTDLLSLLA"
     gene            complement(5680..6321)
                     /locus_tag="PKK36_06885"
     CDS             complement(5680..6321)
                     /locus_tag="PKK36_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HNR94319.1"
                     /translation="MRRNLLLSILLLAATGCQSVYFGAMEKMGVHKRDIMVSRVKAAR
                     DAQDETKEQFVSAMEAFKSVVNFKGGDLEKEYNRLNAVLQKSEAGAQEVRDRIRAVED
                     VSRALFREWRGEIKQYSNRELARSSQEKYDLTKGRYEDLIAAMKKAESKLEPALTPLR
                     DQVLYLKHNLNARAIAGLSSELISVQTNVDRLVRDMEASIAEADRFISTLQDQ"
     gene            6360..8465
                     /locus_tag="PKK36_06890"
     CDS             6360..8465
                     /locus_tag="PKK36_06890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR94320.1"
                     /translation="MAGVNIKFMASPALKVRIAIRCAPGYIEFREWIPVMGTDLTART
                     VRWLVLLCLTAAGGSLCAQSPAEPLKLAGYVEVTSPQIRLSVPDADVAFRAGDNIPLF
                     QENADYFVGIISGEAPFRRLMTVRRPIWADPPVWVTKEGRMVFGSAASSSRGHVEFEA
                     GKKLGVAAVEGKAYQVLLKSGEHLIRLDVPVNKRGLGELVAVRSEWRRKPAQRVIAMT
                     SKTNVPDVTARASASTGAPPESAAEPVPDDAPGEDVSKLSRMLQMAREHMDRPDSTNA
                     PSAPEADKEDTVEETGDAERTWALPQWWPYALSLFFAVALLPLYRRYRRQRAQAPPRP
                     PKPKKEKKPSRKKKISREKKKTVKVSPAPPEDVEPAPAPAQDVTETLPLAKRSAQEIP
                     VVEAKSAEPSAEGFMIGRYQAKSVQGRGTTGIVYKAWQEDLKRTVALKVLKEGRHASM
                     EAKLRFLREAQALARLRHPNIITVYEVGEIDGQPFFSMDFVDGTSLDKLIMTSRPGLR
                     DSVELCRRIAETVHYAHENGIIHRDLKPGNIIVDYRGEPVITDFGLARNLDSGVLSMS
                     GDIIGTPSYMSPEQAQGKTAEVDVRTDVYSIGAVLYSLLTGKSPFARASLLETLNAVI
                     NEAPESIRRLNAQVDRELAGICMKAMAKNKAQRYATAEKLADALARYLAVPPLQRRWR
                     QVHQSLRAVFRKKPVPENP"
     gene            8536..10269
                     /locus_tag="PKK36_06895"
     CDS             8536..10269
                     /locus_tag="PKK36_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94321.1"
                     /translation="MLTLTIVKGPGTGQIFPLDKPEVMIGREPGDGITLEDLSVSRRH
                     FRLILGTPTPEIEDMGSRNGTLLNGKPVTRHELHTGDIIEIGQTTLRFDLIKTREEDM
                     NFDANFNATATIPMHVLRTEVLGIRDAESRDVAALNKARNALAAANRAGQAISAILRT
                     EELYHKAVEIVLDEILHVDRCAVLLKSPNSPLFSPASWSVRGTPAGPEVFPYSRFLAK
                     YTCDEQMAVLIQDMLHDERFQAEDTVRSQCIRSAICVPLISKSNLLGVLYADTLGDAA
                     QFSQDDLKLLAMISLQLCTALETARLYENLLEEKAATETAHRELKETQEKMLQHEQMA
                     AIGEFSAGLVHDLKNPLTVILGHTQFCQLQFARQGDVPPDFRDRICRSLEEIGKGVSH
                     TNHIVEQLLEFARKSMPNKEEVHINGLVNEVLEFIAPEAERAAVTLERKLQADIPVIR
                     LDHAQIKRAFINIVMNAIQAMPNGGTLTVETSLQRRRTGVEVLVRFTDTGVGMAPEAA
                     RRVFQPFFTTKTKTGEGYTGTGLGMAVTYGIIESHEGSISLTSKKGEGTTFFVALPIE
                     LPPAVTQRIRL"
     gene            complement(10304..11599)
                     /gene="thiC"
                     /locus_tag="PKK36_06900"
                     /pseudo
     CDS             complement(10304..11599)
                     /gene="thiC"
                     /locus_tag="PKK36_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(11619..12074)
                     /locus_tag="PKK36_06905"
     CDS             complement(11619..12074)
                     /locus_tag="PKK36_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR94322.1"
                     /translation="MAAYRRLEDLDVYQKLCRLHIEVCALTQRWPTEERFELGSQVRR
                     SSNSTAAQIAEKYDDRHIRNKIEGVNRSRGEAAETIHHLYMALLKGYESRETYETYRS
                     KYQECIRMLNGLERTLEKQLPQSDRRWPDPAVKEPPEEYDAHPSGYPEP"
BASE COUNT         2448 a         3605 c         3726 g         2500 t
ORIGIN      
        1 cgactggtgg cacaagtatc ttgaggtgct gaccaacggc accattgtgc agtttgccat
       61 catgatgaag gacgcctacg ggacggagtt ctgggacaac aacggcggca gtgattacgt
      121 cgtcaccatc ggatcggtcg agccgccgcc gcagggcggc gacaagccgt attcaacgaa
      181 cccgtcactg gggcagcgcg gcaccatgac cattgacggg aatccgtccg gatggaccac
      241 aaatcacctg attgccctgg atatggccaa cgacgatccg cggtccctgt ccagcaactg
      301 gaccatgcat gaggcgccga tcgacctgac gcacatgtgg gcggcctggg atgacgataa
      361 tctgtatctc gcctggcagt atgtggacgt gactgacgtg attgatcctg ccaacgcggg
      421 cggcgcggcc agcgggaaaa tttccaacaa cgacggtatt ctgcaatgga ttgtcataga
      481 cacgaaggcc agcgaggggg ccaccacgga cgtgtggggc aagaagaaca gctgggtggg
      541 ggccaacaag cctgattacc agatatacct tgcgggcagt ctctggcagg gctacatctc
      601 gcgcgcggtg gatggcgtat tcgctttgga cgatggcgga acaaactaca acaccatcgc
      661 cgcggcggga ataacggctg ccaaggggag caccttcggc gccgccagtc tctggggcgt
      721 cggggatgca gacaaccgct ttgacggcgg cgcacccacg cgcaacttcc tgagcgaggg
      781 gcacagcacc agccgcgaca gcttctacga gatgagcatc ccgctcagtt atctcgaaat
      841 caccgctgct cagctcgatt ccggcgccgg catcggcatt atgatcggcg gcggttcgga
      901 atccagtatg gactccattc cgcacgatga aaccacgctg gatctgccgg gggttgaaag
      961 ctacaactcc tcctttgaat ggggagacag cgacaacttc acttcgccgt tctcgcgcat
     1021 cggccactga gcgggcctgt gtttttccaa tgactggaag tttcccggca aaaacttcca
     1081 gtcattggaa cttttctccg cagacttgca gtgattggaa ccttttcccg gccggaaaat
     1141 atttgcggga aatgcagttg acggagccgg cctgatcgga tattttcgcc ggacttttcc
     1201 gcgggaacgt ggacggacca aaaaataagg aaaaaaatga cggcaccgac aaagaacaaa
     1261 aagcttctcg catgggtggc ggaggtcgaa aagatgtgca ccccggaccg tgtggtctgg
     1321 tgcgaaggca cgaaatccga gtacgaccgg ctgatgaacc agatggttga atcgggttcg
     1381 gccgtccggc tcaatgaaaa actgcggccg aacagttttg ccttcaattc cgatccgtcg
     1441 gacgtggctc gtgttgagga ccgcacgtat atcgcctccc gcacaaagga ggaggccggc
     1501 ccgacgaaca actggattga tccggtggag ctcaaaaaaa cgatgcgcgc gctctacacg
     1561 ggatgcatga agggccgcac gatgtacgta atcccgtttt caatgggccc gatcggctcg
     1621 ccgatcgcga agatcggcat tgaaatcacc gacagcccct acgtggtctg caacatgcat
     1681 atcatgaccc gcgtgagcaa gaaggttctg caggtgctgg gcgacgacgg cgagttcatt
     1741 ccctgcctgc attccgtggg cgcgcccctg gagccgggcc agaaggatgt gccgtggccc
     1801 tgcgcgccga ttgagaagaa gtacatcgct catttcccgg agaagaacct gatctggtcg
     1861 ttcggctccg gctacggcgg caacgccctg ctggggaaga agtgcctcgc gctgcgcatt
     1921 gcgtcgaata tggcgcgcaa cgagggctgg atggccgagc atatgctcat cctccgcatg
     1981 atcaatccgg aaggccgcaa gtatcatatc gccgcggcgt tcccgagcgc ctgcggaaaa
     2041 accaacctgg ccatgctgca gtccacgatt cccggctgga aggtcgagac gatcggggat
     2101 gacatcgcct ggatgaagat tcgtccggac ggccgcctgc acgcgatcaa tccggaatac
     2161 gggtttttcg gcgtcgcgcc cggaacgtca aaggcctcca atccgatggc gctggcctcc
     2221 tgcagcaaga acagcatttt caccaactgc gtggtcacca gcgaaggcga catctggtgg
     2281 gaagacatcg gctgcgagtg caggagcgga acggactgga aagggcagcc cttccattcg
     2341 ggcatgaagg acaaggacgg cagggcggtc aagggcgcgc atccgaacgc gcgcttcacg
     2401 gccccggcat ctcagtgtcc cgtgatctgt ccggactggg aggatcccga gggggtgccg
     2461 attgacatct tcatcttcgg cggccgccgc accaccacgg tgccgctggt gcatgaagcg
     2521 ctgagctggg agcacggcgt gttcatgggt gccacagcgg cctccgaacc caccgccgcc
     2581 gcgctggatg tgaaacagca tctgcggttt gatcccttcg cgatgaaacc cttctgcgga
     2641 tacaatgcgg ccgattattt cgcccactgg ttcaagatgg gcgcgcgaat ggacgacaag
     2701 gccccgcgta ttttctatgt caactggttc cgcaagaaca aggacggaaa atggctgtgg
     2761 cccggtttcg gcgagaacag ccgcgtgctc aagtggatgt gcgagcgcgt ggacggccgg
     2821 gttgaggcgc gcgagacgcc tgttggcctg atgccgaagg acggcgatct cgacctgaag
     2881 ggcctgaaca tctccccgga tgattatgcg gaactgatga aggtggatgc gggcctgtac
     2941 cggaatatgc tgtcggatat tgccgattac ctgaaggaat tcggggaccg gcttccctcc
     3001 cagatgacgc atcagcttga agcgctgagg aaacggctcg attagcggcg cctgccgctt
     3061 tcagccgggc cacggcccag cgggcggcct tctgtttcgg agtcagcccg gccatggccg
     3121 ggctggtcga gggcaggcgg atatattcca gagtcttcag ttcggccggc aggtgcggtt
     3181 tcacgcgccg gacgtattcc ctctccgccc gcgccccgtt gaaaagaaca gtctggattc
     3241 ccggatgttc ccgaaagaag gatacgaaat cgttcacgat gattgagcgg tcatcaatgg
     3301 atgcatcaag actcccgtcc cgccggcatt ctttcaggac atcccacagt gcgatgccgc
     3361 agcgcgcaag acgccggcat cggtgcgcat aggttgcgtc gggggagaat ccgaacaagc
     3421 ggcccatgat gggccagaag gcgtttcgcg gatgggcata gtactgccgc tgccgcagcg
     3481 attcctcccc cggcatgctc cctagaatca gcacacacgg ccggccatcg gcaatgggcg
     3541 gaaatccctt tttcatgccc tcaatgatgg cgcggtttct gccggtgtcc agaaattccg
     3601 cacggcttga ttcaatttga tttgtgaatc cgcccgggag aagtatcttc tgcggcaatg
     3661 aagcagtttt ggacagaacc cgccccggcg gatgtggaag cggcccggcg tcggacgctg
     3721 aaactttccg tgtgggacgg cgccttctgg tccgtcatgg ccggattcgg agacatgtat
     3781 ctttctccgt ttgcggtttt cctcagggcg ggaagctggg ccatcgggct gctgacgacg
     3841 atgcccccgc tgctgggcgc gctcgcgcag atgatcggtg cgcactgggc cgaccggctg
     3901 ggccgccgcc gcgacctgat tgtcccgctg atcatgctgc atgcccttgt gtttctcccg
     3961 atattcttcg ttccgctgct gttccggtcc gcggccgtcc cggccctgat tctgcttgcg
     4021 gccctggggg tgatgttcaa catggcggcc gcgccgatct ggacgagcat gatgggggaa
     4081 gtggtgcccg gcgcgacacg cggcgactac tggggccgcc gcagccgcat actgacgctg
     4141 accgtgctgc tggcatctct gacggccggc ggcatcctgt cccttttcca gcgggcgggg
     4201 ctcacatggg cgggattcgg cattcttttc agtgtggcgt ttctggcgcg tttcatctcg
     4261 gcattgatcc tcaggcggca ttatgatccg ccttatgcgc cggggccgga ggcgcatttc
     4321 acattcctgg atttcatccg ccggatgccc cattccaact tcgcccgcta tgcgctgtat
     4381 ttttcgctga tgatgggcgc atcgaacatc gcggcgccct ttttcaccgt ctacatgctt
     4441 cgggatctga agtgggatta tgcccactac acactgtcgc aggccgtatt cctgctgaca
     4501 caggttctgc tggtggggtg gtggggcaga atcggcgacc ggtacggcaa ccgggtcgtg
     4561 attgcggcga ccagcttcat tctcccgctg ctcccgtttt tgtggatgtt cagcacgaat
     4621 tattcctatc tgcttgtcgt gcagcttgtg tcggggatcg gctggtcggg gttcaccatc
     4681 gcggcacaga atttcacgtt cgatgcagtg tcagtccaga agcgcgcccg tgtcgccgcc
     4741 tgtgtgagcg tgcttcacgg gctctgcatg tttgcaggag gcactctgat aggagcgcct
     4801 ctggccaatc acctgccgtc tgtctacacg ctggggccgc tacgggtcgg tttcatctct
     4861 tctcttcccg gcctgtttgc cgtttcgggc ctggcgcgtt tcgccgtggc ggcgctcctg
     4921 ctgcggaggt ttgcggaggt ccgggacgga cacgagcaga ttcatcccgc tcagctcatc
     4981 cttcggctgg ccggcggcgg tattattgcg ggattcttga acgggctgct caggccagaa
     5041 gggaaagaag gtccgtcggc cggctgagca gggcccgcgc tccggcctgc cgcagttcat
     5101 cctctgtgcg gaatccccag agcgcgcctg cggggaaaaa tccggcgcgc acggcggtct
     5161 gcatgtccac atcactgtcg cccagataga ggacttcttc cggccgaagg ccgagatgcc
     5221 gcgtcatggc cagtgctccg gcggggtcgg gttttacggg aacacccggc tgctcgccgc
     5281 gaacggggtc gaaggtccat cgggaaagaa atcgtttcac gcagagttcg accgcggcct
     5341 ggggcttgtt gctgagcacg gccatgcgga ctccgttctg atgcagtgca tcaagcagct
     5401 gcggaatgcc gtcgtagagc cgtgttttat ggtcccatcg cgcatcatat tccccgcgaa
     5461 agtccttgta gcaggcctgc acggtgtctt ccgtccttct ctccggagga atggcgcgtg
     5521 tgatcagtgt aatggcgccg ctgccgacaa aatgacgata ggccttgatc ggatggaccg
     5581 gcagcccatt gcgggccagg gccgcattgg cagaatccgc cagatcctga agggtgtcca
     5641 gcagggtccc gtcgagatcg aaaaagacag cgcgtatctt cactggtcct gcagggtgga
     5701 gatgaagcgg tcggcctccg caatggatgc ctccatgtcc cggaccagcc ggtccacatt
     5761 ggtttgcacg ctgatcagtt cgctgctgag tccggcaatg gcccgcgcat tgagattgtg
     5821 cttgagatag agcacttggt cgcgcagcgg ggtgagggcc ggctccagct tcgactcggc
     5881 ctttttcatc gccgcgatga gatcctcata ccttcccttc gtcaggtcgt atttctcctg
     5941 gctggaacgc gccagttccc ggttggagta ctgcttgatt tccccgcgcc attcgcggaa
     6001 cagggccctg gatacatctt caacggcgcg gatgcggtcg cgcacctcct gtgcgccggc
     6061 ttcgcttttc tgcagcacgg cattaagacg gttgtattcc ttttcaaggt cgccgccctt
     6121 gaagttcacc acgctcttga aggcctccat ggctgagacg aactgctctt tggtctcgtc
     6181 ctgcgcgtcg cgcgcggcct tgacgcggct gaccatgatg tcgcgcttgt gcacgcccat
     6241 tttttccatg gcgccgaaat ataccgactg gcagcccgtg gccgccagca gcagtatgct
     6301 caataaaaga ttgcgtcgca taacacagtc tcccttctta ttccccctgc atcctatccg
     6361 tggccggagt aaatatcaag ttcatggcga gtccggcgct gaaagtgcgc attgccattc
     6421 gttgcgcgcc cgggtatatt gaattccgcg agtggattcc ggttatggga acagacttga
     6481 cggcccggac agtgcgatgg cttgtcctgc tttgtctcac ggcggcagga ggctctctct
     6541 gtgcccagtc cccggcggag ccgctgaagc tggccggcta cgtggaggtg acatccccgc
     6601 agatcaggct ctccgttccc gatgcagacg tggcatttcg ggccggcgac aacatccctc
     6661 ttttccagga aaacgcggac tattttgtcg gcatcatcag cggggaggct cccttcaggc
     6721 gcctaatgac cgttcgccgg ccgatatggg ctgatccccc ggtctgggtg actaaagaag
     6781 gccgcatggt tttcggcagc gcagcatcct ccagcagggg ccatgttgaa ttcgaggctg
     6841 gaaagaaact gggtgtcgct gcagtcgaag ggaaagccta tcaggtgctg ctgaaaagcg
     6901 gagagcacct gattcggctt gatgtcccgg tgaacaagcg cggtctgggt gaactggtcg
     6961 ctgttcggag cgaatggcgc cgaaaacccg cacagcgtgt gattgccatg acctctaaaa
     7021 caaacgttcc ggacgtgacg gcgcgcgcat ccgccagtac cggcgccccg ccggagtccg
     7081 ccgctgagcc cgttccggat gacgctccgg gggaggatgt ctccaagctg tcaagaatgc
     7141 tgcagatggc gagggagcac atggaccggc cggacagcac caatgctccc tcggcgccgg
     7201 aggccgataa ggaggacacg gttgaagaaa ccggcgatgc agagcggact tgggcactcc
     7261 cgcagtggtg gccctatgcg ctttcactct tttttgcagt ggccctgctt ccgctctacc
     7321 gcaggtatcg caggcagcgg gcgcaggctc cgccgcggcc gccaaaaccc aaaaaggaaa
     7381 agaaaccctc cagaaagaaa aaaatatcac gtgagaaaaa gaaaacggtc aaggtgtctc
     7441 ccgccccgcc ggaggacgtg gagcctgcac ctgcgccggc tcaggatgtg acggagaccc
     7501 tgcctctcgc gaagcggagt gcgcaggaaa ttccggtggt tgaagcaaag agcgcagaac
     7561 cctctgccga gggattcatg atcgggcgtt atcaggcgaa gagcgtgcag gggcgcggaa
     7621 ccaccggtat tgtgtacaag gcatggcagg aggatctcaa gcggacggtt gcgctcaagg
     7681 tcctgaaaga aggccggcac gcctccatgg aggccaaact ccgctttctg agggaggcgc
     7741 aggcgctggc ccggctgcgc caccccaata tcattaccgt gtatgaagtg ggtgaaatcg
     7801 acggccagcc gttcttcagc atggattttg tggatggaac ttcgctggac aaactgatca
     7861 tgaccagtcg tcccggcctg cgcgattcag ttgaactctg ccgccggatc gccgaaaccg
     7921 ttcattacgc gcatgaaaac ggaatcattc accgggatct gaagcccgga aacatcatcg
     7981 tggactacag gggggagccg gtcatcaccg acttcggtct cgcccgcaat ctggacagcg
     8041 gcgtgctgtc aatgtcgggc gacatcatcg gcacgccctc ctacatgtct cccgagcagg
     8101 cgcaggggaa aaccgcggaa gtggatgttc gcacggatgt gtattccatt ggcgcggtgc
     8161 tttattccct gctgacaggg aaaagcccgt ttgcccgggc ctcgctcctg gagacgctca
     8221 atgcggtgat taatgaagcg cccgaatcca ttcgcaggct gaatgcccag gtggaccgcg
     8281 aactggcggg aatctgcatg aaggccatgg cgaaaaacaa ggcgcagcgc tatgcgacag
     8341 cggaaaagct ggcggatgcg ctggcccgtt atctggccgt gccgccgctg cagcgccgct
     8401 ggcggcaggt gcaccagtct ctccgcgcgg tgttcaggaa gaagcctgtt cctgaaaacc
     8461 cgtaaatact tctggaatcg cggctcatct ttggtacttt cttctccacg ggccggcggt
     8521 cggccggaaa acagcatgct gacgttgacc atagtgaaag ggcccgggac cgggcagata
     8581 ttcccgctgg acaagccgga agtgatgatc ggccgtgagc cgggcgacgg catcacgctc
     8641 gaggatcttt ccgtttcccg ccggcatttc cgcctgattc tcggcacacc cactccggaa
     8701 atcgaagata tggggagccg caatggaacc ctgctgaacg ggaaacccgt gacccgtcat
     8761 gagctgcaca ccggggatat cattgaaatc ggccagacca ccctgcgctt tgatctcatc
     8821 aagacccgcg aagaagacat gaacttcgac gcgaacttca acgcgaccgc cacgatcccc
     8881 atgcatgtgc tgcgcacaga ggtcttgggc atccgggatg ccgaaagccg cgacgtcgcc
     8941 gcgctcaaca aggcgcgcaa cgcgctggcg gcggccaacc gggccggtca ggcgatcagc
     9001 gccattctcc gcacagaaga actgtatcac aaggcagttg agattgttct ggatgaaatt
     9061 ctccatgtgg accggtgcgc tgtgcttctg aaaagcccga acagcccgct gttcagtccc
     9121 gcatcatggt ctgtgcgcgg caccccggcg ggtccggagg tatttccgta cagccggttt
     9181 ctggccaaat acacctgtga cgaacagatg gccgtgctga ttcaggacat gctgcacgat
     9241 gagcgttttc aggcggagga tacggttcga agccagtgca tccggtctgc catctgtgtg
     9301 cctctgataa gcaaaagcaa cctgctgggt gtgctgtacg ccgatacact cggagacgcg
     9361 gcgcagttca gccaggacga tctgaagctg ctggcgatga tcagcctgca gctctgcacc
     9421 gcgctggaaa cggcccgcct ctacgagaac ctgctggaag aaaaagcggc cacggaaacc
     9481 gcgcaccgtg aactgaagga aactcaggag aaaatgctgc agcatgagca gatggccgcc
     9541 atcggggaat tttccgccgg gctggtgcat gacttaaaga atcccctcac tgtcattctg
     9601 ggccatacgc agttctgcca gcttcagttt gcccgtcagg gggacgtgcc tccggatttc
     9661 cgcgaccgga tatgcagaag tctggaggaa attgggaaag gtgtttctca taccaaccat
     9721 atcgtggaac agcttctgga gttcgcccgg aaatccatgc cgaacaagga ggaagttcac
     9781 atcaacggtt tggtaaatga agtgctggag tttattgctc ccgaggctga gcgggcggcc
     9841 gtgacccttg agcggaaact tcaggcggat attccggtca tccggcttga ccatgcgcag
     9901 atcaagcgcg ccttcatcaa tatcgtgatg aatgccattc aggccatgcc gaatggagga
     9961 accctgacgg tggaaacctc gttgcagcgg cgcagaaccg gagtcgaggt tcttgtccgc
    10021 ttcaccgaca caggggtagg catggccccg gaagccgccc gacgggtctt tcagcccttt
    10081 ttcaccacca aaacaaaaac aggcgaagga tacacgggca ccggactggg catggctgtg
    10141 acctatggca tcatcgagag ccacgagggg agtatttccc tgaccagcaa aaagggcgag
    10201 ggcaccacat tcttcgttgc gcttccgatc gagctccctc cggccgtcac gcagcgcatc
    10261 cgattgtagc cggagcggcg tccgatcagt ccgtatcgcc aatttcctga ctgatgcgca
    10321 tcgaacagaa cttaggcccg cacatggtgc agtaatgcgt ggagccgtcc ttgacgtccg
    10381 gatgcgtttt cgtttcttcc agcgcttctg cccggagcgc gcgggccttt tcggggtcca
    10441 gcgccagttc aaactgcttt tcccagtcga acgtgaccct tgcgcgggag atggcatcgt
    10501 cgcgatcgcg agcatgcggc cgcctgagcg cgatgtcggc cgcgtgagcc gcgattttgt
    10561 atgcgatgac cccttcgcgc acatcccgga cattgggcag gcccagatgc tccttggggg
    10621 tgacatagca gagcatggat gcgccgtaat aggcgcccag ggtcgcgccg atggcggatg
    10681 tgatgtggtc gtacccgggc gcgcagtcgg tgaccaccgg cccgagaata taaaaaggcg
    10741 cgccgtcaca gatcgcatct tctttttcca tgttcatctg aatctggtcc agcgggacat
    10801 ggcccggtcc ttcaaccatg gcctgcacgc cgtccgcgcg cgagcgacgc accagttcac
    10861 ccagcgcctg cagctccgca aactgcgcct catccgaagc atccgccaga cagccgggcc
    10921 ggagcccgtc acccagcgaa agcgtgacat cgtgcgccct gcagatctca agcatccggt
    10981 cgaagtgctc gtagagcgga ttttcctttt tgtgccgggt catccacttt gcgaggatag
    11041 aaccgccgcg gctgacaatg ccgatcagcc ttttcttcgc cagcggcaca tgctcccgaa
    11101 gaaggcccgc atggaccgtg atgtagtcca caccctgctt cgcctgggct tcaataacct
    11161 ccagaagaag ctcggcggta atgtccatgg gatcatccac catggcaagc gcctgatata
    11221 tgggcaccgt gccgaccggg accgtgcttt ccgcgatgat gcgcgcgcga atctcgttga
    11281 tgttttctcc ggtgctgaga tccatcacgg tgtcggcccc gtattgaatg gcgatcttca
    11341 tcttctcgaa ctcttcgcac aggccgctgg tggtggcgga attcccgatg ttcgcgttga
    11401 tcttcgtgga aagcgctctg ccgatgccga tgggatccag ccggacatga tggcggttcg
    11461 agggaattac gattcggccg gccgcggttt catcgcggat gaactccggc ttcagatatt
    11521 ccttctgcgc caccgcgttc atcgcatcgg tgatgattcc gtcccgcgcc gcggcaatct
    11581 gggttttgaa tgttgccata gttgtcagtg ttcagtgttc agggttcggg atatccggag
    11641 ggatgggcgt catattcttc ggggggttct ttaacagcag ggtccggcca tcggcggtcg
    11701 gattgaggaa gctgcttctc cagagtgcgt tctaatccgt tcaacatgcg tatgcattcc
    11761 tgatacttac tgcgatatgt ttcataggtt tcccgggact cataaccttt caggagcgcc
    11821 atgtaaaggt gatgaattgt ttccgccgcc tctccccggc tccgattgac cccctctatc
    11881 ttgttgcgaa tatgccggtc atcatatttt tctgcgatct gggccgctgt gctgtttgaa
    11941 gaacgcctga cttgtgaccc cagctcgaac ctttcttcag tcggccagcg ctgtgtaagg
    12001 gcgcagacct cgatatgcag acggcatagt ttctgataaa cgtccaaatc ttctaaccgg
    12061 cgataagcag ccattcactc tcctgtgcaa ttccactcct gaaccctgaa ccctgaaacc
    12121 cgcaatccat tatctaaatc cgcgccggcc gtgtgtcaag gagggtgctt ccgaaaacct
    12181 taaaaacatg ctcaaaatgg cctaaaaatg ctcaaaaaca gcccaaaacg gccaaaaatc
    12241 gtaaaaaaac gtcaaaaatc accaaaaacg ggctgaaac
//
LOCUS       DAOQWR010000070        12272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5644_length_12272_cov_5.321683, whole genome shotgun sequence.
ACCESSION   DAOQWR010000070 DAOQWR010000000
VERSION     DAOQWR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5644_length_12272_cov_5.321683"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2723)
                     /locus_tag="PKK36_06910"
     CDS             complement(<1..2723)
                     /locus_tag="PKK36_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94323.1"
                     /translation="MEKENNRWSAAALWLLLLAGLALRVWGAWAFRHITDPDCGVVAL
                     MAKHMAEGRAWPVFFYGQSYMGSLEPALSALCCRLLGISGFAVGLGTALAAAGALVFT
                     YLWGREAAGRPGGLAALALCIIGPPGFFLFQFAPRGGYMVALMLAVYVMWHASRLSRR
                     LIRREKIASWHFLLLGFGAGLGWWSNQIITSALVAAAAVLAIGWRGRLFNRGVLLGLA
                     GFIAGSSPFWIWNAMHGWDSFNMLTNLGAIDPARGFRHLLVKSWRFIGAADANPVAAV
                     AFATLTGVTILAGAAVTGWRFRRSGEWRGASFCALVFILVSCGFFIRSAYAVMNTTRY
                     LVPLVPAAAVLAAGAISALPRRGGSALPITLILLLMLPQVQVLKQVALKAQESSRGPE
                     QISELSEFLNQNGVEAVYAHFRYYPFNFHLDERWPFTDMRGDRVPALARRAELAQNIA
                     VYDNHGQIDTFLKVCGGDADHTQVAGRRMTQNFRSPLQNLREIASTSISGIRDGRGND
                     LTEKVTDRNADTFWESALGDAREEDVEIAFSEPVKVARIRLLAPSERFYPRNLKIKAV
                     FEGDESWTPIYAVHAVPRFHWSGSRPYWAGRRFRLEYRLPAGRPMVRLRLSAPKEAVL
                     ERSLWAISEIQFFAPAPGEQSGAQPYAELAGLLERRGIRRLYSDRGPANVLNRIDGFQ
                     VELEMEPAFFPDQALCRIGEINWSPHTAFLVLRQDAPVAEETLAAFDIRMRQTEIGPW
                     VLFDFENAESVPRGHVPVHWTGFNLLKGQGREHARFELDRAFSIMNEQGDPAEAMTAL
                     SEAVEFYPNSVTVSRELIDWLAKTRTVEEMEAVYSEAARPRTPVRAEFENGVELAGVS
                     FPDDPVTPGDEVLVQWFWRCPPDVLPSDYAVFVHFRGGTQRFQDDHILLEH"
     gene            2847..3500
                     /locus_tag="PKK36_06915"
     CDS             2847..3500
                     /locus_tag="PKK36_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94324.1"
                     /translation="MNGTNSNEGQPEYKPLDFGDVLKRAFDLYKENLGLLIVVHLVGG
                     LLSLCTIGILAGPLYAGVSLVMLALYDRKDPKPVVGDLFKGFDFFLPAFLLFLVVCAV
                     MIPVSMITGPLSSIAGLVAGTVTMFSYFLIVERKMDFWPAIVESYNMVKENFWVYLGL
                     YVVGWIGSALGFIACCIGIVVTAPYLSCVTVIAYRETRPTVRDAVVSQDMAPNTSMA"
     gene            3505..3909
                     /locus_tag="PKK36_06920"
     CDS             3505..3909
                     /locus_tag="PKK36_06920"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNR94325.1"
                     /translation="MRLNIQKEPSRLRAARENAAWLVLTLPFLAAPYLRPSEMLPDLC
                     LFRKLSGVPCMFCGFTRAFENMACGRFREALTACPAAVPLYAGLWIAFAAALALTFQG
                     RSLKVILSGREQKILLAAAALLLLANWAWRLI"
     gene            complement(3916..4596)
                     /locus_tag="PKK36_06925"
     CDS             complement(3916..4596)
                     /locus_tag="PKK36_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNR94326.1"
                     /translation="MSRIPRKAFWWSFAAHVAVILSLIVVPAVMNCRMRMKPAEIITY
                     VDLQNLTVPVAAPEVPAVQPEPPPPEPRKDIPEPAPVKKKKIEVSKKKIKREETPAPP
                     KSELTPEEIRKLLAAGAKPSPRPGPELDLPAWYFALVRQMMYDQWNQPGELAGAAGLM
                     TRVQFRIHRDGSISHIKMIRASGSKVMDESVMTAVNAVKRLKELPASYREAHYDATVD
                     FELTSGGF"
     gene            complement(4593..5012)
                     /locus_tag="PKK36_06930"
     CDS             complement(4593..5012)
                     /locus_tag="PKK36_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR94327.1"
                     /translation="MARHTSLTSLKQISEINLTPLMDLTFILLITFIITFPLIEQGIP
                     VNLPRGKASPLDPDQSRSISLNLEGQIFLDDVMITKEALASEMTLMGQSDPETTVLVR
                     ADEGIRYGQLVEIMQILHDAKIARLALVTQQEEKPAK"
     gene            complement(5025..5759)
                     /locus_tag="PKK36_06935"
     CDS             complement(5025..5759)
                     /locus_tag="PKK36_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR94328.1"
                     /translation="MTVLPIHYFPLADVGFAFRESNLAGQLIVLILLVGSVFAWTIMV
                     SKVRELNRAGRLTDLFMHQYRKESHPLNLFLRQKRFAGSPVYEIYEKACLGVAAENDR
                     LSRAGSLFAAEEAASLRLTTTQIEGIRNLAERNVADQALLLENNMGYLATAVSTAPFL
                     GLLGTVWGVMDSFGGMARTGAPTLAAVAPGISGALLTTVVGLLVALPSAIGYNWITNR
                     IRTLCVQMDNFAQEFSADIQRNFGAE"
     gene            5897..6817
                     /locus_tag="PKK36_06940"
     CDS             5897..6817
                     /locus_tag="PKK36_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR94329.1"
                     /translation="MILGIDIGGTKTSVCAGDADGRILFSARMPSLPERGPDDWMKRL
                     FRLAQEVLAESGIDLTDVRSIGISAPGPLSVQRGALLSPPNMTGWETVPVIAPVQDHF
                     GRPVYMNNDANAGVLAEWLWGGCAGTKDMIFLTMSTGLGAGIIAGGTLVQGACDMGGE
                     AGYHVLDPMGPASPCGHRGSFEAFCGGKNAADRFSRRMKYPRPADFRHFMDEVRRKNP
                     IALEEWEIYIEHLAQGIGNLIMILNPSIILLGTIAMHTGSIIFDELSRRLPAYTVPEA
                     LKACRIEAATLGRYLGDHAALAVAAKNMAQ"
     gene            complement(6876..7496)
                     /locus_tag="PKK36_06945"
     CDS             complement(6876..7496)
                     /locus_tag="PKK36_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94330.1"
                     /translation="MKKDAQTIYRQRIAAALLCPLALLTALLNGGLLPLKRSYDDAVA
                     AIDQLARKIAPARETIDRSLVLAKEEELLDRKLVRVLKAGAYLAEDIFMHLNLEIYDD
                     ARQAKLDIAQLKTRTIAAENWMGGTRAGFHLRPFGIFVDARGSFNEIRQFLELLEKNN
                     PFAAVSSLTIRKMSPEDTEYRLEMTAEWPVLTEKFLTEVKPTISGL"
     gene            complement(7493..8143)
                     /locus_tag="PKK36_06950"
     CDS             complement(7493..8143)
                     /locus_tag="PKK36_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94331.1"
                     /translation="MRNGLKKEPDNQPLPRAVRYAAVFMYATCIPVLIGLIYYGILSS
                     WKRNLLEEQRSEWRNMAHTCASALDQQQRFDAKKQTLEELGGWHNARIVWNEVLTALE
                     TQMPTDISILTVRGQGELQSLSPPQGDQLHTRPRIGREHTLQASFIGGAGWDEARIPG
                     MLRSLRTAHGISRYFPYVNIGGVVKDNRDAEQENAGPAAGKIVDFYASGGIRMINP"
     gene            8280..8747
                     /locus_tag="PKK36_06955"
     CDS             8280..8747
                     /locus_tag="PKK36_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR94332.1"
                     /translation="MKTAAHRRKQGYSLMELMVVIGILGIIMSVAAMDYVKGMPKRRV
                     HGAAFDIVVRLRQARMMAASANAPAEVSISGRTITTWVDRDGDGVRDAGEESSMEIDP
                     LVNFFNYPNSGTFDARGMFNSPGSYLYIYINAPNVVGQTVYVFPSGHVDLYYY"
     gene            8772..9128
                     /locus_tag="PKK36_06960"
     CDS             8772..9128
                     /locus_tag="PKK36_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR94333.1"
                     /translation="MKKSSKKGFTIVEVVIALAIFSIGVMGVYGLAAWLIQANDFSNR
                     MTAATSIAQGRLEDIQNQSFPLMASGSETTADNYTVTWTVTSINATLKSILVVVTWTD
                     IKGKTHKVELRTMSDS"
     gene            9146..10000
                     /locus_tag="PKK36_06965"
     CDS             9146..10000
                     /locus_tag="PKK36_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR94334.1"
                     /translation="MNNLKPELSTKGKGFTLVEVIVACALSLILVTVMIAAAVNYQRI
                     FKTQNSINDMTQNVRAAMDLVARDMRTAGYGLDDSASANMVNWITWVSGFNANPLVVD
                     GGSGPDTVYIAGAFTEPIGYLTADVPIGGTVLVLDGGGSQVNMTDKKVIFVGRLETAR
                     VIGLAGNTLTISTHPSSAAGLSYSYSAGAPVEVVSLLTYQVVDNPSLYPYGPHLQRID
                     STTPYAYDWQRIAAGNIEDLQVTGNGGTMEIEIQGRTSKPDDGYTDPDYGDHYRRFRL
                     ETTIHRRN"
     gene            10051..11271
                     /locus_tag="PKK36_06970"
     CDS             10051..11271
                     /locus_tag="PKK36_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94335.1"
                     /translation="MKTRKTESAVLSKKAGVALFTVLTLLATLSLLASFSYMVMRTDM
                     SISNNYVKATKAFYQTEAGIHYVRNQIEQLLKSGAPLTVGENAVNIEPPEGFTFDPVH
                     SFTVLDSRMQSFLYSVTGHSGDSKTTIEVVVARRPLIMCGLLGENSLRVQPQFDILSF
                     DSRLLAGLPAFGDSSGEASVGSNYDVTLQSHVVLDGNVLLGAAEDGTWAVFTGHGVGD
                     WETVEFERFDPDPLGSIGGNLADQFTDVIANNDNASADPPIVGNTVNLGPGDTLTLTA
                     GDYYLTSMDMGPKGDLIIDASAGPVNIYLDGEWYMGPNGTINNGGLPTDLSIFSRSDE
                     RIWIQPNSGFSGFIYAPFSTDVWMKPHGDAYGAVWAKDLRLFPQGYWFIDVALLDKFM
                     SDELHFASWKEIRS"
     gene            complement(11504..>12272)
                     /locus_tag="PKK36_06975"
     CDS             complement(11504..>12272)
                     /locus_tag="PKK36_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94336.1"
                     /translation="NCTIIGNTTTNLGGGGYCFEGGLIAGSIVLSNNASFSVSNWHRN
                     DANAVFSYNCSMPLLPGIGNITNDPRFVDAVATNFQLLYGSPCIDSVFGIAAGPDLDG
                     VLCPQDGNYDGTNDYDMGCYEYDPATADSNGDLIPDWWYIQYSMNPLNITMALGNADG
                     DMLDNGDEYVANRDPTDDTSYFYISNVVYGSACNMSVECSASRLYDLEFKDNPYDITW
                     EPVPGQTDIPGDAGGSLTLIDTNNIQGRIYRIGVHLP"
BASE COUNT         2715 a         3517 c         3437 g         2603 t
ORIGIN      
        1 atatgttcca gcaggatatg atcgtcctgg aatcgctgtg tccccccccg gaaatgcaca
       61 aacaccgcat agtcggaggg gaggacatcc ggagggcacc gccagaacca ctgcaccagg
      121 acttcatccc cgggagtgac cggatcatcc gggaaactga cgccagccag ttcgacgccg
      181 ttctcaaatt ccgcgcgcac cggcgtgcgg gggcgcgcag cctcgctata gaccgcctcc
      241 atttcttcga cggtccgggt ttttgcaagc cagtcaatca gttcgcgcga aaccgtgaca
      301 gaattgggat aaaattcaac cgcttcagac agagccgtca tcgcctccgc cggatcgccc
      361 tgttcgttca tgatgctgaa ggcgcggtcg agttcgaatc gggcatgctc cctcccctgc
      421 cccttgagca gattaaatcc ggtccagtgc accggaacat gcccccgcgg aacagattcg
      481 gcattttcaa aatcaaacag cacccagggg ccgatctccg tctgccgcat gcggatgtca
      541 aacgccgcaa gcgtttcctc cgcaacagga gcatcctgcc gcagcaccag gaaggcggta
      601 tgaggcgacc agttgatttc accgatccgg cagagcgcct ggtccgggaa gaaggctggt
      661 tccatttcga gttccacctg aaatccgtct atccggttca gcacattggc cgggccgcgg
      721 tcgctgtaca aacgccggat tccccgccgc tccagcaggc cggccagttc ggcataaggc
      781 tgggcgcctg attgctctcc cggcgccgga gcaaaaaact ggatttcaga aatggcccac
      841 agagatcgct cgagcacagc ctccttcggc gccgacaggc gcaatcggac catcgggcgg
      901 ccggcgggca ggcggtattc cagccggaaa cgccggccgg cccagtaggg tcgggacccg
      961 gaccagtgaa aacgcggaac ggcatgcacc gcatagatgg gcgtccagct ttcatccccc
     1021 tcgaacaccg ccttgatttt caggttccgc gggtagaacc gttcagacgg cgcgagaaga
     1081 cgaatacgcg ccaccttcac aggctctgag aatgcaatct caacatcctc ttcacgggca
     1141 tcccccagcg cactttccca gaaggtgtcg gcattgcggt ccgtcacctt ctccgtcagg
     1201 tcattgccgc gaccatccct gatgccggaa atggaggttg aggcaatctc gcgcaggttc
     1261 tgcagcggtg acctgaaatt ctgcgtcatg cgacgaccgg ccacctgggt gtgatcggca
     1321 tcgccgccgc agaccttgag aaaagtatca atctgaccgt gattgtcata gaccgcgata
     1381 ttctgcgcga gttcagcccg gcgcgcgagg gccggcaccc ggtccccccg catgtcggtg
     1441 aatggccagc gttcatctaa atgaaagttg aaaggataat agcggaaatg cgcatagacc
     1501 gcctcgaccc cgttctggtt gaggaactcc gagagctcgc tgatctgttc aggccctctg
     1561 ctgctctcct gcgccttcag cgcgacctgt ttcagaacct gtacctgcgg gagcatcagc
     1621 agaaggatca gcgtaatcgg gagagcagag ccgccgcggc gcggcagtgc ggaaatcgca
     1681 cccgcagcca gaacggccgc ggccggcacc agcggcacca gatagcgggt ggtgttcatc
     1741 acggcatatg cggaacggat gaaaaagccg caggaaacca gaatgaaaac aagcgcgcag
     1801 aacgacgctc cgcgccattc tcctgaccgc cggaatcgcc atcccgtcac cgcggctcct
     1861 gccaggatgg tcaccccggt cagggtggcg aaagccacgg cagccacggg gttcgcgtcc
     1921 gccgcgccga tgaaccgcca tgatttcacc agcaggtgcc ggaaaccccg cgcggggtca
     1981 atggcgccaa gattcgtcag catgttgaag gagtcccagc catgcatggc gttccatatc
     2041 cagaaagggc tgctgccggc aataaatcca gccaggccca gaagaacgcc gcgattgaac
     2101 agccggccgc gccagccaat cgccagaaca gccgccgcag cgaccagcgc cgaagtgata
     2161 atctggtttg accaccagcc gagcccggcg ccgaacccga gcagcaggaa atgccaggaa
     2221 gcaatctttt cgcggcggat cagcctgcgc gaaaggcgcg aggcgtgcca catcacatag
     2281 acggccagca tgagagccac catatagccg ccgcgcggtg caaactgaaa aaggaagaag
     2341 ccgggcggac caataatgca aagcgcgagg gcggccagcc cgccgggccg gcctgccgct
     2401 tcccgtcccc agagatacgt gaaaacaagc gcgcccgcgg cagcgagggc tgtgcccagg
     2461 cccaccgcaa agccgctgat ccccagcagg cggcagcaaa gggcgctcag agccggttca
     2521 aggcttccca tgtagctctg gccatagaag aaaaccggcc atgcgcggcc ctcggccatg
     2581 tgctttgcca tcagggccac cacaccgcag tccggatccg tgatgtgccg gaaagcccat
     2641 gcgccccaaa cccgcagcgc caggcccgcc agaagcagca gccacaacgc ggcggcagac
     2701 cagcgattat tctctttttc cacttccctc attgatgcgg gagtgtagca caaacccgtg
     2761 attccactgc aagaaaaacg ggacagcttc gtgcttgaag atttcgcatt ttagttcaat
     2821 atgttccttc aacaaaggag tctgccatga acggaacgaa cagtaatgag ggtcagccgg
     2881 aatacaaacc gctggatttc ggcgatgtgc tgaaacgcgc tttcgacttg tacaaggaaa
     2941 acctgggact gctgatcgtg gtgcatctgg tgggcggcct gctgtccctc tgcaccattg
     3001 gcatcctggc cggaccgctg tatgccgggg tttcccttgt catgctggcc ctctatgaca
     3061 ggaaagaccc caaaccggtc gtcggtgact tgttcaaggg ttttgatttc ttccttccgg
     3121 cttttcttct tttcctcgtc gtgtgcgccg tgatgatccc ggtgtccatg ataaccgggc
     3181 ccctgtcctc gattgccgga ctggtggctg gaactgtcac aatgttttcg tactttctga
     3241 ttgtggagcg caaaatggat ttctggcccg cgatcgtgga aagctacaac atggtcaaag
     3301 agaatttctg ggtttatctg gggctgtatg tggtgggttg gatcggctca gcgctgggat
     3361 tcattgcctg ctgcatcggc attgttgtca ccgcccccta tctgtcctgt gtgaccgtga
     3421 ttgcctatcg cgaaacccgc ccgaccgtca gggatgcggt tgtttcgcag gacatggcgc
     3481 ccaacacaag catggcctga aggcgtgcgg ctgaacattc agaaggagcc gtcacgcctc
     3541 cgcgcagcgc gcgaaaacgc ggcatggctt gttctgactc tgccgtttct tgcggcccca
     3601 tatcttcgcc cctcggagat gctcccggat ctctgcctgt tccggaagct gtccggcgtt
     3661 ccctgcatgt tctgcggctt cacccgggcc tttgaaaaca tggcctgcgg ccgtttccgc
     3721 gaggccctga cagcgtgccc cgccgccgtc ccgctttatg cgggtctttg gattgcattc
     3781 gcggcggcgc tggcgctgac gtttcaggga cgaagcctca aggtcatcct ctcgggacgt
     3841 gagcagaaaa tcctgcttgc ggcggcggcg ctgcttctgc tggccaactg ggcctggcgc
     3901 ctgatttgaa agggatcaga atccgcccga ggtgagttca aagtccacgg tggcgtcata
     3961 gtgggcttcg cgatacgagg cgggaagctc cttgagccgt ttgacggcat tcacggcggt
     4021 catgacggac tcgtccatca ctttgcttcc cgaagcgcgg atcattttga tatgggaaat
     4081 gctcccgtcg cgatggattc gaaactgcac acgggtcatg agccccgccg cgccggccag
     4141 ctctccgggc tggttccact ggtcatacat catctgacgc accagcgcga agtaccatgc
     4201 gggaagatcc agctccggcc cgggccgggg tgaaggcttc gcgcccgccg ccaggagctt
     4261 cctgatctcc tccggcgtga gctcggactt tgggggcgca ggtgtttctt ccctcttgat
     4321 cttctttttg ctgacttcga ttttcttttt cttcaccggc gcgggttcag gaatatcctt
     4381 ccgcggctcc gggggcgggg gctcgggctg aaccgccggg acttcgggcg ccgcaacagg
     4441 aaccgtcagg ttctgcagat ccacataggt gatgatttcc gccggcttca tccgcatgcg
     4501 gcagttcatg acggccggaa caacaatgag gctcagaatc accgccacat gggcagcaaa
     4561 gctccaccag aaagccttcc gcggaatccg gctcatttcg caggtttctc ctcctgctgc
     4621 gtcaccagcg ccaggcgggc gattttcgcg tcgtgcagga tctgcatgat ctccaccagc
     4681 tgcccgtagc ggatgccctc gtcggcgcga accagcacgg ttgtctccgg atccgactgg
     4741 cccatcagcg tcatttccga tgccagcgcc tcctttgtga tcatcacatc atcaagaaaa
     4801 atctgacctt caaggttcag gctgatgctt cgggactgat ccggatccag cggactggcc
     4861 ttcccgcgcg gaagattgac ggggatgccc tgctcgatga ggggaaaagt gatgataaag
     4921 gtgatcagca gaatgaatgt aagatccatc agcggggtga gattaatctc actgatttgc
     4981 ttgagtgacg tgagtgaagt gtgccgggcc actgccgtct cctcctattc cgctccgaaa
     5041 ttgcgctgaa tgtcggccga gaattcctgc gcgaaattat ccatctgaac gcaaagggtt
     5101 cgaatgcggt tagtgatcca gttgtagccg atggccgagg gaagcgcgac aagaaggccc
     5161 accacggtgg tcagaagggc gccggaaatt ccgggggcca ccgcagccag cgtcggggct
     5221 ccggtgcgcg ccatgccgcc aaaggagtcc atgacccccc acaccgtgcc cagcaggccc
     5281 agaaaaggcg ctgtactgac cgccgtggcc agatatccca tgttgttttc gagcaggagc
     5341 gcctgatccg ccacattgcg ctcggccagg ttgcggatgc cctcgatttg cgtggtggtc
     5401 agccgcaggc ttgcggcctc ttccgcggca aagagactcc cggcgcgcga gagccggtca
     5461 ttttcagccg caacgcccaa acaggccttc tcgtaaattt catataccgg gctcccggca
     5521 aacctctttt gtcgcaggaa aagattcagc ggatggcttt ccttcctgta ctgatgcatg
     5581 aagaggtccg tgagtctgcc ggcgcggttg agttcccgca cctttgagac catgatggtc
     5641 catgcgaaaa cggaacccac cagaagaatc agcacgatca gctgtccggc cagattggat
     5701 tccctgaaag caaatccgac atccgcgagc ggaaaataat gaatgggcaa gaccgtcatc
     5761 acttcatgtc ctctgattga aatactctgt attctctgaa ctgaaaccgc actctattac
     5821 atggcgcgcc ggcggtcaat ccggagctgt ccggatttgg acttgagttt gcgccggcaa
     5881 aaggaaagat ggtttcatga ttcttggaat cgacatcggc gggaccaaga cgtccgtgtg
     5941 cgccggagac gcggacgggc ggattctatt ttcggcgcgc atgccgtcgc ttccggagcg
     6001 cgggccggac gactggatga aacggctttt caggctggcg caggaggtgc tggccgaatc
     6061 gggaattgat ctgaccgatg tccggtccat cgggatctcc gcgcccggac cgctgtccgt
     6121 ccagcgcggc gcgctgcttt caccgcccaa catgaccgga tgggaaacgg tgcccgttat
     6181 cgctcctgtt caggatcatt tcggaagacc tgtctacatg aacaacgatg ccaatgccgg
     6241 agtgctggcc gaatggctct ggggcggctg cgcgggaaca aaggacatga tcttcctgac
     6301 catgagcaca ggcctcggcg cggggatcat cgccggaggg acgcttgttc agggggcctg
     6361 cgacatgggg ggcgaagcgg gatatcacgt gctcgatccc atgggccctg catcaccctg
     6421 cggccaccgc ggcagttttg aggccttctg cggaggcaag aacgctgccg accggttcag
     6481 ccgcaggatg aaatatcccc ggccggccga tttccggcat ttcatggatg aggttcgcag
     6541 gaaaaacccg atcgcgcttg aggaatggga aatctacatc gagcatctcg cgcagggtat
     6601 tggcaacctg atcatgatcc tgaatccctc gatcatcctg cttggcacca ttgcgatgca
     6661 tacgggaagc atcatctttg atgaactgtc gcggaggctg ccagcctaca ccgttccgga
     6721 agcgctcaaa gcatgccgga ttgaggcggc aacgcttgga agatatcttg gagaccacgc
     6781 ggctctggct gttgcagcta agaatatggc ccaataattc tcataaatga gaatttttta
     6841 atccagccag cctgcggtcg aaatatttct atattttata aaccagaaat agtgggcttc
     6901 acttctgtga ggaatttttc ggtcaatacc ggccactcgg cggtcatttc cagccgatat
     6961 tccgtgtctt caggactcat tttgcggatc gtgagagatg aaacagctgc aaaaggatta
     7021 ttcttttcca aaagctccag aaactgcctg atctcattga aactcccacg cgcatccacg
     7081 aagattccaa acggtcttaa gtgaaaaccc gcgcgtgtcc cacccatcca gttttctgcc
     7141 gcaatggtcc gcgtcttcaa ctgggcgata tcaagtttag cctgccgggc gtcatcataa
     7201 atctcaagat tcagatgcat gaaaatatcc tcggccagat acgcgccggc ctttaacacc
     7261 cgcaccagct tgcggtccag cagctcttcc tcctttgcca gcaccagcga acggtcaatg
     7321 gtctcccggg caggtgcaat ttttcgagca agttgatcaa tagcggcgac ggcgtcatca
     7381 taactgcgct tcagcggcag cagtcctcca ttgagaaggg ccgtcagcag cgccagcgga
     7441 caaagaagcg cagccgcgat ccgctgcctg tatatcgttt gggcatcttt cttcatggat
     7501 tgatcattct tattccgcca gaggcataaa aatcgacaat tttccctgcg gccggaccgg
     7561 cattctcctg ttcagcatcg cggttgtcct taaccacgcc gccgatattc acatagggaa
     7621 aatatctgct gattccatgc gcagtgcgca gagacctcaa catgccggga atccgggctt
     7681 cgtcccatcc ggcgccgcca atgaaactgg cctggagcgt gtgctcgcgg ccgatacggg
     7741 gccgggtgtg aagctggtcc ccctgtggtg gagagaggct ctgcagctcc ccctgccccc
     7801 ggactgttaa aattgaaata tccgtcggca tttgcgtctc aagggccgtc agcacttcat
     7861 tccatacgat ccgagcattg tgccacccgc caagctcctc cagcgtttgt ttcttggcat
     7921 cgaaacgctg ctgctgatca agggcggaag cgcacgtatg cgccatattc cgccattcag
     7981 aacgctgctc ctccagcaga tttcttttcc atgaggaaag aatcccgtag tagatgagtc
     8041 ctatcagcac cggaatgcag gtcgcgtaca taaaaacagc cgcataacga acagcacgcg
     8101 gaagcggctg attgtccggc tcctttttca aaccatttct catgtggaca atatgcctga
     8161 gcgcgggtga aaacagaagc ttttttctcc ggttccctga atgcggctta tattatttct
     8221 ttttactatg cattggcatg aattttgctt atattaagta tgtcggtatg gggtacatta
     8281 tgaaaacagc ggcacacaga cgaaaacagg gttactcgct gatggaactc atggtggtga
     8341 tcggcattct ggggatcatc atgtcagttg ccgccatgga ttacgtcaaa ggcatgccca
     8401 aacggagagt gcacggcgcg gcctttgaca tcgtggtcag gcttcgacag gcccggatga
     8461 tggcggccag cgccaatgcc cccgccgagg tcagcatttc ggggcgcacc atcaccacgt
     8521 gggtcgaccg cgacggggac ggggttcggg atgcaggcga ggaatccagc atggaaattg
     8581 atcctctggt gaattttttc aactatccga attcgggcac cttcgacgcc cgcggaatgt
     8641 ttaattcccc gggttcgtat ttgtacattt acataaacgc tccaaatgtg gtggggcaga
     8701 cggtttatgt gtttcccagc gggcatgtgg atctctacta ttactgatat gacgaatgaa
     8761 ggcggtttga tgtgaaaaaa tcatcaaaaa aaggttttac gattgtggag gtggtgatcg
     8821 cccttgcaat tttctccatt ggggtgatgg gcgtttacgg gctggcggca tggctgattc
     8881 aggccaatga tttcagcaac agaatgaccg cggccacttc catcgcacag ggcagactcg
     8941 aagacatcca gaaccagtct ttcccgctca tggccagcgg ttcggaaacc accgccgaca
     9001 attacaccgt cacctggacc gtgacctcca tcaacgccac attaaaatcc attctcgttg
     9061 tcgtaacctg gacggacatc aagggcaaaa cccataaagt tgaattaaga accatgtcgg
     9121 acagttgaag agcagaggaa gagcaatgaa caacttgaaa ccagaactga gcacgaaggg
     9181 caaaggtttc actctcgtgg aagtgatcgt ggcctgcgca ctcagcctga ttctggtcac
     9241 cgtgatgatc gccgcggccg tcaactatca gcggattttc aaaacgcaga actccatcaa
     9301 cgacatgacg cagaacgtgc gggccgcgat ggatctggtt gcccgcgaca tgcgcaccgc
     9361 cggttacggg ctggacgatt ccgccagcgc caacatggtt aactggatca cctgggtctc
     9421 cgggttcaat gccaatccgc tggtcgtcga cggagggagc ggaccggata ccgtctacat
     9481 tgccggggcc ttcacagaac ccatcggtta tctgaccgca gacgtgccga ttggcggcac
     9541 cgtgctggtt ctggacggag gaggttcaca ggtaaatatg acggacaaaa aagtgatctt
     9601 tgtgggcagg ctggaaaccg cccgcgtaat cggactggcg ggcaacaccc tgaccatcag
     9661 cacccatcca tcaagcgcag ccggactcag ctacagttat tcagccggag cgcctgtgga
     9721 agtggtctct ctgctcacct atcaggtcgt tgataatccg tccctgtatc cctacgggcc
     9781 ccatttgcag cgcatagaca gcacaacgcc atacgcatac gactggcagc gaatagccgc
     9841 cggcaacatt gaggaccttc aggtaacggg aaacggaggc accatggaaa tcgagatcca
     9901 ggggcgcaca tcaaaaccgg atgacggata tacagatccg gattacggcg atcactacag
     9961 gcggttcagg ctcgagacaa ccattcacag aagaaactga ttggggtatg gtaatggcag
    10021 gggcagagag ccccggaagg aaggtgcgaa atgaagacgc ggaaaacaga aagtgctgtt
    10081 ttatcaaaga aggccggagt tgcactgttt acagtgctga ctttgctggc cacgctgtcg
    10141 ctgctggctt ctttctcata catggttatg aggacggaca tgtccatcag caacaattac
    10201 gtcaaggcga caaaggcgtt ctatcagacg gaggccggaa ttcattatgt gcgcaatcag
    10261 atcgagcagc ttctcaaatc cggcgccccg ctgactgtgg gcgaaaacgc tgttaacatc
    10321 gagcccccgg agggattcac ctttgatccg gtgcacagct tcaccgtgct ggattcgcgg
    10381 atgcagtcct ttctctattc cgtcaccgga cattccggcg actcaaaaac gaccattgag
    10441 gtcgttgtgg ccaggcgccc gctaatcatg tgcgggctgc tgggagagaa cagcctgcgg
    10501 gtgcaacccc agtttgacat cctgagcttt gattcacggt tattggcagg gctgcccgcc
    10561 tttggggaca gttccgggga ggcatctgtc ggctcgaact atgatgttac actgcaaagc
    10621 catgtggtac tggacgggaa cgttcttctt ggtgccgcag aggacggcac atgggctgtt
    10681 ttcacgggac atggggtcgg cgactgggaa accgttgaat ttgaacgatt tgatccagat
    10741 cccctgggct caataggcgg aaatctggcg gatcagttca ctgacgttat tgccaataat
    10801 gacaatgcgt ctgctgatcc ccccatcgtt gggaacacgg taaacttggg ccccggtgat
    10861 acccttacac tgaccgcagg agactactat ctgacatcta tggatatggg tcctaaaggc
    10921 gatttgatca tagacgcatc cgccgggccg gtgaatatct atcttgacgg cgagtggtac
    10981 atgggtccga atggaactat taataatgga ggactgccca cggatttgag cattttttca
    11041 cgctcggatg aaagaatctg gatccagcca aacagcgggt tttcagggtt catttacgcc
    11101 ccattttcca ctgacgtctg gatgaagcct cacggcgatg cctatggtgc tgtgtgggcc
    11161 aaggatcttc gcttattccc tcagggatac tggttcattg atgtggctct gctggataaa
    11221 tttatgtcag acgaactgca ttttgcatcc tggaaagaaa ttcgcagcta aaagactttc
    11281 cggattgctt caatggacga ggacttgcct ccgtgcgcat ggatattctt gcggacgacg
    11341 gatccctttg gatcttgctt cccgtcaatc cacaaacatc aggttgcgct tcaacttctc
    11401 tggcagacca gaaaaacatt gagccctccg caacatttcc agatcgccac agactgaaat
    11461 catagtgcct ggggcgggca tctgtgcttc ccatgttgca cacctatggc aaatgcacgc
    11521 ctatccggta aatgcgtcct tgaatattat tggtgtcgat cagcgtcaga gaaccgcctg
    11581 catcccccgg tatgtccgtt tgacccggca ccggctccca ggtgatgtca taaggattgt
    11641 ccttgaattc caggtcatag agccgcgacg ctgaacattc gacactcata ttgcatgcag
    11701 agccatagac cacattggaa atataaaaat atgatgtatc atcagttgga tccctgttcg
    11761 caacatattc atcaccgtta tccaacatgt caccgtctgc gtttcccaga gccatcgtta
    11821 tattcagagg attcatactg tattgaatat accaccagtc agggatcaaa tccccatttg
    11881 aatcggccgt ggcgggatca tactcataac agcccatgtc ataatcgttg gtcccgtcat
    11941 agttcccgtc ctgaggacaa agtacgccgt caaggtcagg gccggcggcg ataccaaaaa
    12001 cagaatcaat gcatggcgag ccgtaaagca actgaaagtt ggttgcaacg gcatccacaa
    12061 agcggggatc gtttgtgatg ttgccgattc caggcagcag tggcatagaa cagttgtagc
    12121 tgaaaacagc attggcgtca ttcctgtgcc agttggagac ggaaaaagat gcattgtttg
    12181 ataaaacaat gctgccggcg ataaggcccc cctcaaagca ataccccccc ccccctaggt
    12241 tggtggttgt attcccgatg atggtgcagt tg
//
LOCUS       DAOQWR010000071        12159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5709_length_12159_cov_5.594845, whole genome shotgun sequence.
ACCESSION   DAOQWR010000071 DAOQWR010000000
VERSION     DAOQWR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5709_length_12159_cov_5.594845"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..3271
                     /gene="secA"
                     /locus_tag="PKK36_06980"
     CDS             245..3271
                     /gene="secA"
                     /locus_tag="PKK36_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNR94337.1"
                     /translation="MKWLLKKIIGTKNERDLKKIRPLVERINQLEQEFQSLSDDALKA
                     KTAEFRERLAQGETVDDLMCEAYAVVKNACRRLCGTRTDVCGHELLWEMVPFDVQLIG
                     GIALHRGMIAEMATGEGKTLVATMPVYLNALPGKGVHVVTVNDYLARRDAEWMGTVYR
                     YLGLTVGCIQNQMHPAERRRQYACDITYGTNSEFGFDYLRDMGMATRPEDQVQRGYAY
                     AIIDEIDSILIDEARTPLIISGPAPYSTHRYAEMKPAVSRLVRKQQDLCSRLIREAKE
                     HLEKNEDEDAQLKLYQVRQGMPKNKQLLHMMEEPSIRKLIEKIENAMLTDMRKDQARA
                     LREELYFTIDERGHDANLTEKGCEALSPDDPEAYVLPDLVTFLAELDGDTTLSEADKI
                     KKRQEMQDAFAERSERIHNVDQLIRAYCIYEKDVEYVVQNNQVIIVDEYTGRLMPGRR
                     WSDGLHQAVEAKEGVTIERETQTLATITIQNYFRMYDKLGGMTGTAETEADEFHQIYK
                     LDVLVIPTNRPIRRVDANDHIYKTQREKFKAVIDEIRDCHARGQPVLVGTVSVDTSEL
                     LSRMLTRENIPHNVLNAKNHEREAEIVSRAGLPGQVTIATNMAGRGTDIKLGEGVVYL
                     GRDVITSSLTLDSRVDGKTLRETLIEKPCGLHVIGSERHESRRIDRQLRGRCARQGDP
                     GSSRFYVSLEDDLMRLFGSDRVANIMGRLGLEEGEVLEHPWLNRSIETAQRRVEQQNF
                     SIRKRTLEYDDVMNKQREVIYGLRRDVVSGSNVREQLLDIVDDVIDDRVEAALEADTE
                     EALADLVNWMNMTFPVGIRAEDLRDVSKDGAALADLLRDRVRKTYDLKVQQEDQKQIG
                     AMERWLILQPIDTHWQEYLRGMDALRQGVGLRAYGQKDPLVEYKHEAYEMFAELMDRI
                     KNDVVSKMFRIATSAQVFENLRAALPQRYVHQEVQTLGGRSQEAPPGAEAAMQAALRS
                     KGEPIRREEAKVGRNDPCPCGSGKKYKKCCGAKAGA"
     gene            3317..4921
                     /gene="lnt"
                     /locus_tag="PKK36_06985"
     CDS             3317..4921
                     /gene="lnt"
                     /locus_tag="PKK36_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNR94338.1"
                     /translation="MAASIPLLSAVASGLLLAAAYPPLEYSELAWFAFVPLMLSLRKA
                     GRGEAARLGFSSALAFWCCSLFWLRHVTWAGWFTLAAYCALYTVPFAVFMAWGLSARR
                     GRLWNVALMFMAAAVWTALEYLRSTLFTGFAWNALGVSQYERLTIIQISRFGGVYAVS
                     ALIMFLNTAIAATVMHYLHEVIPSRHAAARSAAVRWHPELVIAFLALALIHMDGARYI
                     GKYRPSSQTLRAALIQPNIPQIEKWSAEHISTIYDRLERWTGAAMKVENYDLTVWPET
                     ALPDEVRYSPASFALIESLVTNGIPLLAGSMDAVMPESGEVNYFNSSFLFSPEGRVVM
                     GYDKQHLVLFGEYVPFRTVLPFLSALTPISESFSAGTESSVFTLEGGQKFSVLICFED
                     TVASLARKALKAGGRLLINQTNDAWFDVSSGSRQHMAHCVFRCVENDVAAFRAANTGV
                     TCAIDARGRLLDVWGSLPLPRRTEGFRIYELPVRAESEPFTFYTLRGDLFAQACLVLA
                     GCVWLIAWTTKGRARPPVCSSPGCGS"
     gene            4943..6056
                     /gene="prfB"
                     /locus_tag="PKK36_06990"
     CDS             join(4943..5011,5013..6056)
                     /gene="prfB"
                     /locus_tag="PKK36_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121874281.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNR94339.1"
                     /translation="MLEELKERLQKYLNRVEEMRGYLDVAERQSQLEKLEQAASAPDF
                     WNDQNKARDIIRETNAVRAVLNPYQELLAGLHDAGEMLELLEMEEDGAQVQQALPGLN
                     DQLDGLEKQLDALELKSLLSGPLDASNAYLTIHAGAGGTESCDWADMLYRMYRRYLED
                     HGYEAEIIDYQPGDEAGIKSISLQVTGPYAYGYMRAERGVHRLVRISPFDSNKRRHTS
                     FASLDVVAQVAEDVEIEINEDDLRIDTYRAGGAGGQHVNKTDSAIRITHLPTGIVVQC
                     QAERSQHSNRAKAYDMLRARLYEYQMDQKRKEMERFYGTKGEIAWGHQIRSYVLQPYT
                     MVKDHRTDVEVGNVQAVLDGALDVFIEAYLKKTVGQ"
     gene            6053..6829
                     /gene="trpC"
                     /locus_tag="PKK36_06995"
     CDS             6053..6829
                     /gene="trpC"
                     /locus_tag="PKK36_06995"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNR94340.1"
                     /translation="MSILDEIADNKRAEIAKRRASCPIETLRGRGRGDFAAAMKAAGI
                     GLVAEVKRKSPSAGLIRRPLVPADVAAAYEKAGAQAVSVLLDEKYFGGGEGDFVEVRR
                     AVSLPMLYKEFVVDEWQIRHAASLGASAVLLIAGILNAVEIEGFMACARDCGLDVLAE
                     VHDAQEVDKIKPLQPAVVGINNRDLKTFKVDIETTFRLKDRLAGDALIISESGIRSAS
                     DVKRLHDNGIGGILVGEQLLRQPDIETAVKELMKEVRPCS"
     gene            6820..7419
                     /locus_tag="PKK36_07000"
     CDS             6820..7419
                     /locus_tag="PKK36_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNR94341.1"
                     /translation="MFVKICGLCSGEDAAAAADLGADALGFIFWKPSKRFVAAGDVAA
                     WTAGFPAEILKVGVFVDASPDEIARTVNTAKLDVVQLHGSLKRPSPGIRIWRVVYPGK
                     TPAFSPAEVDAVILDSYSESSPGGTGVCCDWDAAARIVESAEVPVILAGGLNPENVAV
                     AVARVRPWGVDVSSGVERAIRVKDLAKVKEFILNAKSAG"
     gene            7400..8611
                     /gene="trpB"
                     /locus_tag="PKK36_07005"
     CDS             7400..8611
                     /gene="trpB"
                     /locus_tag="PKK36_07005"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNR94342.1"
                     /translation="MQRVPDKRGHFGPYGGMYVPETLVSPLEELTRAYAAARRDPRFR
                     SEIQDLLKNYVGRPTPLYFARRMTRELGGPKIYLKREDLCHTGAHKINNCIGQAILAR
                     RMGKTRIIAETGAGQHGVATATVAALLGLECVVYMGKVDMERQALNVFRMERLGARVV
                     PVTAGQQTLKEAISEAMRDWVTNVHNTFYILGSVLGPHPYPMMVRDFQSVIGREAHRQ
                     CLAEEGNLPSALVACVGGGSNAMGLFYAFLKDKSVKMYGVEAGGLGLRTGRHAARFHD
                     GRVGVLQGTQTYVLQDARGQVRLTHSVSAGLDYAAVGPEHSLLRDMKRVEYSSVSDSE
                     AVKAVDALCIWEGILPALESAHAAAFVMKHADRWRKKDIVIINLSGRGDKDVAQLTEW
                     KMTSPRKRRTS"
     gene            8608..9492
                     /locus_tag="PKK36_07010"
     CDS             8608..9492
                     /locus_tag="PKK36_07010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNR94343.1"
                     /translation="MTVKSMTGFGSAKAVGRGFEILVEISSVNRKQADIQLDLPRQLV
                     CVESLFGEEIRKIVSSGRVNCKVQMNQSGGGSCRNMHIDEKLAQHYLSRIRSAARRLK
                     LAQEVSTEYLMGLPGVVVFYEPKADRDVLWPVCRRALSLALRQFDLMRRTEGAALAED
                     IASRLTLMEKLIRRIAVRAPQTVRQYRKTLMQRISSAGVDTADSDGRLLREIALFADR
                     CDISEELTRLDSHFKQARSLMAGKNPAGRSLDFLAQEMLREFNTIGSKAHDAVIRGTV
                     VTCKAELERLREQVQNIE"
     gene            9489..10118
                     /gene="gmk"
                     /locus_tag="PKK36_07015"
     CDS             9489..10118
                     /gene="gmk"
                     /locus_tag="PKK36_07015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNR94344.1"
                     /translation="MNTSNWKPLIFAVSAPSGAGKTTLCMRLLKEFPNLTYSVSCTTR
                     HPRPGEVDGKDYHFITQQDFDRRVEAGEFLEHASVHGSSYGTLRSEVEGAVARGRDVL
                     MDIDVQGATQIRSSMNAMDEDHHLRRSYFDIFIVPPSMEVLRMRLTGRGSDEDDVIRR
                     RLAKAEDEMKHWKEYQYLIVNDDLEKAYDALRAVFVAAHHRVDAMDQSL"
     gene            complement(10215..10685)
                     /locus_tag="PKK36_07020"
     CDS             complement(10215..10685)
                     /locus_tag="PKK36_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94345.1"
                     /translation="MLYMTALHQHIGAPEARRGFLQCYFFFFATFFFATFFFATFFTA
                     FLAGAFFFATFFFAAFFAGAFFATFFFAAFFFAAISASPFVSGCYTGMFLFSPGRTAA
                     LSRRSPPDVYPSLFRFISDDYLLVEIYNANRSTESNYLKYFSQFFHSEYFRFSA"
     gene            10792..11247
                     /gene="nrdR"
                     /locus_tag="PKK36_07025"
     CDS             10792..11247
                     /gene="nrdR"
                     /locus_tag="PKK36_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNR94346.1"
                     /translation="MRCPKCSHLDDKVIDSRTVRSGNAIRRRRVCLSCQHRFTTYEEV
                     VKSTLRVIKRDGRHEELDRKKLMAGVVRACEKRPISLQQIEMMLDSVMNELEAEYDRE
                     VPSTVIGKKIMTHLEKLDEVAYVRFASVYRRFRDVNQFLSEVEGLIGRE"
     gene            11244..11933
                     /locus_tag="PKK36_07030"
     CDS             11244..11933
                     /locus_tag="PKK36_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94347.1"
                     /translation="MIPHTENQALEALVAYIEETLRSTCSGAWTGDESVRQIAGGVAE
                     FCLEQFSSLRIPSAYLALLTARALWAVGEEEASRLFVMAHCPPDIRKICVPFSRRPDE
                     MNRCWKLCAAKILRSSRWVSRSDGLMWTLDMRRIEWSSGQGLELAMRQGLAELIQPIA
                     PVWDSFSGRGELGLLGLREQIGRLINRPPQSKTVQPVAAEMRGLCEDLLMRIGKSRGW
                     QGCPEIVELSL"
     gene            12005..>12159
                     /locus_tag="PKK36_07035"
     CDS             12005..>12159
                     /locus_tag="PKK36_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HNR94348.1"
                     /translation="MSRHPSLKASGKIAAKRNVLKRYERVTLLESRGKWKEGDRGLGL
                     PKTKPAE"
BASE COUNT         2617 a         3265 c         3885 g         2392 t
ORIGIN      
        1 aaatagtact aaaatagtac tatttaacgt gtgtgcggcc gggatatgaa atctttgtgt
       61 attatgcgtg ttttgcggcg attctgttga tccagtcaaa aattccgtat tctgaattct
      121 gtcttctgac tcctgaattc cgactcctga ctcctttctt ctccctcctg tctgcttgcc
      181 aaaaggtgcg ggggtgcgta gtcttaacgg gtttcatata tataaagaaa gcaccggttt
      241 atccatgaaa tggcttttga agaagattat cggcacgaag aacgagcgcg acctgaagaa
      301 aatccggccg ctggttgagc gcatcaatca gctggagcag gaatttcagt cgctttcgga
      361 tgacgccttg aaggcgaaga ccgcggagtt ccgcgagcgg ctggcgcagg gcgagacggt
      421 ggacgacctg atgtgcgagg cctatgccgt ggtcaagaac gcctgtcgcc ggctgtgcgg
      481 cacccgaacg gatgtctgcg ggcatgaact gctgtgggag atggtgccgt tcgatgtgca
      541 gctgatcggc ggcattgcgc tgcaccgcgg catgattgcg gaaatggcga cgggcgaggg
      601 caagacgctg gtggccacga tgcccgttta tttgaacgcg ctgcccggga agggcgtgca
      661 tgtggtgacg gtgaacgact acctggcccg ccgcgatgcc gaatggatgg gcacggtcta
      721 ccgctatctg ggcctgaccg tgggctgcat ccagaatcag atgcatcccg ccgagcgccg
      781 ccggcagtat gcctgcgaca tcacctacgg caccaacagc gaattcggat tcgactatct
      841 gcgcgacatg ggcatggcca cccgtcccga agaccaggtc cagcggggct atgcctatgc
      901 gatcattgac gaaattgaca gcatcctgat tgatgaggcc cggaccccgc tgatcatctc
      961 gggcccggcg ccgtattcca cccatcgcta tgccgagatg aagcccgcgg tgagccggct
     1021 ggtcaggaag cagcaggatc tgtgttcgcg cctgatccgc gaggcgaaag agcacctgga
     1081 gaaaaatgag gacgaggatg cccagctcaa gctctatcag gtgcggcagg gcatgccgaa
     1141 gaacaagcag ctgctgcaca tgatggagga gccttccatc cgcaagctga tcgaaaaaat
     1201 agaaaacgcc atgctgaccg acatgcgcaa ggatcaggcg cgcgcgctcc gcgaagagct
     1261 ctatttcacc attgatgaac ggggacatga tgccaatctg acggagaagg ggtgcgaagc
     1321 gctcagccct gatgatcccg aagcctatgt ccttcccgat ctggtcacgt tcctcgcgga
     1381 gctcgacggg gacaccactc tttccgaagc cgacaagatc aaaaaacggc aggaaatgca
     1441 ggatgcgttc gcggagcgga gcgagcggat tcacaacgtg gaccagctta ttcgcgccta
     1501 ctgcatttac gagaaggatg tcgagtatgt cgtccagaac aatcaggtga tcatcgtgga
     1561 cgagtacacc ggccgcctga tgccgggccg ccgctggagc gacggcctgc accaggccgt
     1621 tgaggccaag gagggcgtga caatcgagcg cgaaacccag acgctcgcga ccatcaccat
     1681 ccagaactac ttcaggatgt atgacaagct cggcggcatg acgggcaccg ccgagaccga
     1741 ggccgacgag ttccaccaga tctacaagct cgatgtgctg gtcattccca ccaaccgccc
     1801 gatccggcgc gtggacgcca acgaccatat ctacaaaacc cagcgcgaga agttcaaagc
     1861 cgtcattgat gaaatccgcg actgtcacgc tcgcggccag cccgtgctcg tgggcacggt
     1921 gtccgtggac acctccgagc tgctcagccg gatgctgacg cgggagaaca ttccccacaa
     1981 cgtgctgaac gccaaaaacc acgagcgcga ggccgagatt gtctcgcgcg ctggacttcc
     2041 gggccaggtg accattgcca ccaacatggc cggccgcgga accgacatca agctcgggga
     2101 gggcgtggtc tatctcggcc gtgatgtcat cacctccagt ctcacgctgg attcccgcgt
     2161 ggacgggaaa acgctgcgcg aaacactcat cgagaagccc tgcggccttc atgtgatcgg
     2221 cagcgagcgg catgaatccc ggcgcattga ccggcagctg cgcggccgct gcgcgcgcca
     2281 gggcgacccg ggatcctcgc gcttctatgt gtcgctggaa gacgacctga tgcgtttgtt
     2341 cggatcggat cgggtggcga acatcatggg gcgcctgggc ctggaggagg gcgaggtgct
     2401 ggagcatccg tggctcaacc gctccatcga aacagcccag cgccgcgttg aacagcagaa
     2461 cttctccatc cgcaaacgca cgctcgaata cgacgatgtg atgaacaagc agcgcgaagt
     2521 gatctacggc ctgcgcaggg acgtcgtcag cggcagcaat gtccgcgagc agctgctgga
     2581 cattgtggac gatgtgattg acgaccgcgt cgaggcggcg ctcgaggcgg acacagagga
     2641 ggcgctggcg gatctggtca actggatgaa catgaccttt cctgtcggca tccgggccga
     2701 agatctcagg gatgtctcca aagacggcgc ggcgcttgcg gatctgctgc gcgaccgggt
     2761 tcgcaaaact tatgacctga aggtgcagca ggaggatcag aagcagatcg gcgccatgga
     2821 acgctggctg atactgcagc ccattgacac ccactggcag gaatatctgc gcggcatgga
     2881 tgccctgcgt cagggcgtgg ggctgcgcgc gtacggccag aaagatccgc tggtggaata
     2941 caagcacgaa gcctatgaga tgtttgcgga actcatggac cgcatcaaaa acgacgtggt
     3001 ttcaaaaatg ttccgcattg ccacttccgc acaggtgttt gaaaacctgc gcgcggcgct
     3061 tccgcagcgg tatgttcatc aggaagtgca gacgctgggc ggccgttcgc aggaggcccc
     3121 tccgggcgca gaggccgcca tgcaggcggc cctgcggagc aagggcgagc ccatccgccg
     3181 tgaagaggcc aaggtgggcc gcaacgatcc ctgcccctgc ggaagcggga aaaaatataa
     3241 aaagtgctgc ggcgccaaag ccggagcctg agcggtcatg acttcagacg ggtgcgcagc
     3301 cagagtccga agccgcatgg cggcttccat tcccctgttg agcgctgttg cttccggact
     3361 gctgctggcg gcggcatatc ctccgcttga atattctgaa ctggcctggt ttgcctttgt
     3421 gccgctgatg ctgagtctgc gcaaagcggg caggggagaa gccgcgcggc tcgggttttc
     3481 aagcgcactg gcgttctggt gctgttcact gttctggctt cgccatgtga cctgggcggg
     3541 ctggttcacg ctggcggcct actgcgcgct ctacacagtg ccctttgccg tgttcatggc
     3601 ctgggggttg agcgcccgca gggggagact gtggaatgtc gcgctcatgt tcatggcggc
     3661 cgccgtctgg acggccctgg aatatcttcg ttcaactctg ttcaccggtt tcgcgtggaa
     3721 cgcgctgggt gtcagccagt atgagcgcct gacaatcatc cagatcagcc gtttcggagg
     3781 cgtatacgcc gtttccgcgc tgatcatgtt tctcaatacg gcgattgccg caacggtgat
     3841 gcattacttg catgaggtga ttccttcccg gcatgccgcc gcccgctccg cggccgtgcg
     3901 ctggcatccc gaactggtga ttgcgtttct ggcgctggcg ctcattcaca tggatggcgc
     3961 gcggtatatc gggaaatacc gcccgtcctc gcaaacgctg cgcgcggcgc tgatccagcc
     4021 gaatatcccg cagattgaga aatggtcggc ggaacatatc agcaccatct atgaccgcct
     4081 tgaacgctgg acgggcgccg ccatgaaggt ggaaaattat gatttgaccg tctggccgga
     4141 gacggcgctg cccgacgagg ttcgctacag cccggccagt tttgcgctca ttgagagtct
     4201 tgtgacaaac gggattcccc tgctggcggg atccatggat gcggtgatgc cggaaagcgg
     4261 cgaggtgaac tatttcaaca gctcgttcct gttcagcccc gaagggcgcg tggtcatggg
     4321 ctatgacaag cagcatctgg tgctgttcgg cgaatatgtc ccgttcagga cggtcctccc
     4381 gtttctttcg gccctgaccc cgatatcgga aagtttctct gcgggaacag aaagttctgt
     4441 gttcaccctg gagggcggac agaagttctc cgtgctgatt tgttttgaag acacggtggc
     4501 gtcgctggcc cggaaagccc tgaaggcggg cggccgcctg ctgatcaacc agaccaatga
     4561 cgcgtggttt gacgtgtcca gcggttcacg ccagcacatg gcccactgtg tgttccgctg
     4621 cgtggaaaat gacgtggcgg cgttccgcgc ggccaatacg ggcgtgacgt gcgcaataga
     4681 tgcgcgcggc cgcctgcttg atgtgtgggg aagcctcccg ctgccccgca gaacggaggg
     4741 cttccggatt tatgagctgc ccgtgcgggc ggagtctgaa ccgttcactt tctacacgct
     4801 ccggggcgat cttttcgcgc aggcttgcct tgttctggcc ggatgcgtat ggttgatcgc
     4861 ctggaccacg aagggtcgcg cccgtccgcc ggtttgttcc tcgcccggct gcggatcatg
     4921 atggggaagg aaagggatgc agatgctgga agaactgaag gaacggctgc agaaatattt
     4981 gaaccgcgtt gaagaaatgc gaggttatct ttgacgtcgc cgaacgtcaa agtcagcttg
     5041 aaaaacttga acaggcggct tccgcccccg atttctggaa cgaccagaac aaagcccgcg
     5101 atatcattcg tgaaacgaat gcggtccgcg cggttctgaa cccctatcag gaactgctcg
     5161 ccggactgca tgacgccggc gaaatgttgg aactcctcga gatggaagag gatggagcgc
     5221 aggtgcagca ggcgctgccc ggcctgaatg atcagctgga cggcctggaa aagcagctgg
     5281 atgcgcttga actcaagtcg ctgctcagcg gtccgctgga cgccagcaat gcctatctga
     5341 ctattcacgc cggcgcgggc ggaaccgagt cctgtgactg ggcggacatg ctgtaccgca
     5401 tgtatcgccg ttatctggaa gatcacgggt atgaggcgga gatcattgat tatcagcccg
     5461 gcgacgaagc gggcatcaag agcatctcgc tgcaggtcac gggcccctat gcctatggat
     5521 acatgagggc cgaaagagga gttcatcggc tggtgcggat cagtcccttt gattccaaca
     5581 agcggcggca cacctcgttt gcgtcgcttg acgtggtggc gcaggtggcg gaagatgttg
     5641 agatagagat caacgaggac gatcttcgca tagacaccta ccgggccgga ggcgccggag
     5701 gccagcatgt caacaagact gactcggcca ttcggattac gcatctcccg accggcattg
     5761 tggtgcagtg ccaggcggaa cgttcacagc acagcaatcg cgccaaggcc tacgacatgc
     5821 tgcgtgcccg gctttacgaa tatcagatgg accagaagcg caaggagatg gagcggttct
     5881 acggcaccaa gggggaaatc gcatggggcc accagatccg gtcctatgtc ctgcagccct
     5941 acaccatggt gaaggatcac cggacggatg ttgaagtcgg caatgtgcag gctgtgctgg
     6001 acggggcgct ggatgtgttt attgaagcct atctgaagaa aacagtcgga caatgagcat
     6061 ccttgacgaa atagcggaca acaagcgtgc ggagatcgcg aagcgaaggg cttcttgtcc
     6121 aatcgaaacc ctgcgcggac ggggccgcgg ggattttgcg gccgccatga aggcggcggg
     6181 catcggtctt gtcgccgagg tgaagcgcaa atctccgtcc gccggattga tccgccggcc
     6241 tctggtgccc gccgacgttg cggcggccta tgagaaggcc ggcgcgcagg cggtttcggt
     6301 cctgctggat gagaaatatt ttggcggcgg cgagggcgat tttgtcgaag tgcgccgggc
     6361 tgtcagcctc cccatgctgt acaaggagtt cgtggttgac gagtggcaga tccggcatgc
     6421 ggcatccctt ggcgcctcgg cggtgctcct gattgcgggg atcctgaacg ctgtggaaat
     6481 cgaaggattc atggcgtgcg cgcgggattg cgggctcgat gtgctcgcgg aagtgcacga
     6541 cgcgcaggag gtggacaaaa tcaaacccct gcagccggcg gtggtcggca tcaacaaccg
     6601 tgatttgaag accttcaagg tggatattga aaccacattc cggctgaagg accgtctggc
     6661 tggagatgcc ctgatcatca gtgaaagcgg tatccggtct gcttccgatg tgaaacgcct
     6721 ccatgacaac ggcattgggg gcattctggt gggcgagcag ctgctgcggc agcccgacat
     6781 cgaaaccgcg gttaaggagc ttatgaaaga ggtgcggccg tgttcgtgaa gatttgcggc
     6841 ctttgttcag gggaagacgc ggcggctgcc gctgatctgg gggccgatgc cctcggtttc
     6901 attttctgga agccttcaaa gcgctttgta gcggccgggg atgtcgcggc gtggacggcg
     6961 gggtttccgg ctgaaatcct gaaagtaggt gtttttgttg acgcatcgcc agatgaaatt
     7021 gcgcgcacgg tcaacacggc gaagctggat gtcgtgcagc tgcacggcag cctgaagcgg
     7081 ccatccccgg gaatcaggat atggcgggtg gtttatccgg ggaaaacgcc ggccttctcc
     7141 ccggcggagg ttgacgcagt tatcctcgac agctacagtg agagttctcc cggaggaact
     7201 ggagtctgct gtgactggga tgccgccgcg cggatagttg aaagcgcgga ggttccggtg
     7261 attctggccg gcggcctgaa tccggaaaat gtggctgtgg ccgtcgccag ggtgcgcccg
     7321 tggggagtgg atgtcagttc tggtgttgag cgggcgatcc gtgtaaagga tttggcaaaa
     7381 gtaaaggaat tcattcttaa tgcaaagagt gccggataag aggggtcatt tcggtcctta
     7441 tggcgggatg tatgtcccgg agacgctggt ttccccgctg gaggagctga ccagagccta
     7501 tgctgccgcc cgtcgggatc cccggttccg ttcggagatt caggaccttt tgaaaaacta
     7561 tgtgggccgg cccacaccgc tttatttcgc ccggcgcatg acgcgcgagc tgggcggacc
     7621 gaagatttat ctcaagcgcg aggacttgtg ccacacgggc gcgcacaaga tcaacaactg
     7681 catagggcag gcgatcctgg cccggcggat gggaaagacg cgcatcattg ccgaaaccgg
     7741 cgccggccag catggcgtgg cgacggcgac agtggctgcg ctgctgggcc ttgagtgtgt
     7801 ggtctacatg ggcaaggtgg atatggagcg ccaggcgctc aatgtgtttc gaatggagcg
     7861 tctgggcgcc cgggtggtgc ctgtaacggc cggccagcag accctcaagg aggccatcag
     7921 cgaggccatg cgtgactggg tcaccaatgt ccataacacg ttctatatcc tcggatcggt
     7981 gctggggcct catccctatc cgatgatggt gcgtgatttc cagagcgtca taggccggga
     8041 agcgcaccgg cagtgtctgg ccgaagaggg aaatctgccg tctgccctcg tggcctgtgt
     8101 tggcggcgga agcaatgcca tggggctgtt ctatgcattc ctgaaggata agtccgtaaa
     8161 gatgtacggg gtcgaggccg gcgggcttgg gctgcgcacc ggccggcatg cggccagatt
     8221 tcatgacggg cgggtggggg ttctgcaggg aacgcagacg tatgtgcttc aggatgcacg
     8281 tggacaggtg cgcctgacgc attcggtgag cgccggcctg gattatgcgg cggtcggacc
     8341 cgagcacagt ctgctgagag acatgaagcg ggtggaatac agcagcgtgt ccgattccga
     8401 agccgtgaag gcggttgacg ccctttgcat ctgggagggc atattgcccg cgctcgaatc
     8461 cgcccatgcg gcggcatttg tcatgaaaca tgctgatcgc tggagaaaaa aggacattgt
     8521 gatcatcaat ctttccggcc gcggggacaa ggatgttgcg cagctcaccg aatggaagat
     8581 gaccagcccc cgcaaaagga gaacatcatg acggtcaaaa gcatgaccgg cttcggatcg
     8641 gcgaaggccg tcggccgcgg atttgaaatt cttgttgaaa tcagttcggt gaaccggaag
     8701 caggcggata ttcagctgga cttgccgcgc cagctggttt gtgtggaaag cctgtttggc
     8761 gaggagatcc gtaagattgt ttcttccggg cgggtgaact gcaaggtcca gatgaaccag
     8821 agcggcggcg ggagctgccg caacatgcat attgacgaga agcttgcgca gcactacctg
     8881 tcgcgcatca ggtcagccgc ccggcggctt aagctcgcac aggaggtgag cacggaatat
     8941 ctgatggggc tccccggagt ggtggtgttc tatgagccaa aagcggacag ggatgtgttg
     9001 tggccggtat gccggcgcgc tctctcgctg gcgctgcggc agtttgatct catgcgcagg
     9061 accgaaggcg ccgccctcgc ggaagacatt gcttcgcggc tgacgctgat ggagaaactg
     9121 attcgccgga ttgccgttcg cgcgccgcag accgtcaggc agtatcgcaa aaccctgatg
     9181 cagcgcattt ccagtgcggg ggtggacacg gccgacagcg atggccggct tctgcgtgaa
     9241 atcgcgctgt ttgcggaccg gtgcgacatc agtgaagagc tgacgcgcct tgacagtcac
     9301 ttcaagcagg cgcgcagctt gatggccggc aagaatccgg cgggacgttc gcttgatttt
     9361 ctggcgcagg aaatgttaag agagttcaac acaatcggct caaaggcgca cgatgcggtg
     9421 atccgcggaa ccgtggtgac ctgcaaggct gaactggaac gactgagaga acaggtgcag
     9481 aacatagaat gaacacatca aattggaaac cgctgatctt cgccgtatcc gccccttccg
     9541 gtgcgggtaa aaccacgctt tgcatgcgtt tgcttaagga attcccgaat ttgacctatt
     9601 ccgtgtcctg caccacccgc catccgcggc cgggagaagt ggacggaaaa gactaccatt
     9661 tcatcaccca gcaggatttc gaccggcggg tcgaggccgg tgagttcctc gagcacgcaa
     9721 gtgttcacgg atcctcatat ggcacactgc gcagtgaagt ggagggggcc gtagccaggg
     9781 ggcgggatgt gctcatggac attgatgtgc agggcgcaac gcagatacgc agttcgatga
     9841 acgcgatgga tgaggatcat cacctgcgac gatcctactt tgacatcttc attgttccgc
     9901 cctccatgga ggtgcttcgc atgcgcctga cgggccgcgg ttcggatgaa gacgatgtga
     9961 tccgccgccg cctcgcaaag gccgaggatg agatgaagca ctggaaagaa tatcagtatc
    10021 tcatcgtcaa cgatgatctg gagaaggcat acgatgcgct ccgcgcagta tttgtcgccg
    10081 cgcatcatcg tgtcgacgcg atggatcagt cgctctaaaa atatttctgc agcttctcgc
    10141 agcagccgtt tcacccttta ttatcagtgg tgcgccgcat gtcgctccgc gcggatgaaa
    10201 tgccatcggc atcgttatgc gctgaagcgg aaatattctg aatggaaaaa ctgcgaaaaa
    10261 tatttcaaat agtttgactc agttgaacga ttcgcattat atatttcaac aagaagataa
    10321 tcatcgctga taaatctgaa tagtgaaggg taaacgtccg gaggggatcg ccggctgagt
    10381 gccgcggttc tcccgggaga gaagagaaac attccggtgt agcagccgga aacgaaaggg
    10441 gaagcagaaa tggcagcgaa gaagaaagca gcgaaaaaga aagtcgcgaa aaaagccccg
    10501 gcgaaaaaag ccgcgaagaa aaaagtcgcg aagaaaaaag ccccggccaa gaaggcagtg
    10561 aaaaaagtcg cgaagaaaaa agttgcgaag aaaaaagtcg cgaagaaaaa gaaataacac
    10621 tgcaaaaatc ctcggcgcgc ttcgggcgcg ccgatgtgtt ggtgtaaggc ggtcatgtaa
    10681 agcacatggc cgccttttct gtacagcctt tcctcttgac cccacaaaat atggtatgct
    10741 atttcttaac gcaccctttg aattgtggag ggctggtcag gagacaggtt tatgcgttgt
    10801 ccgaaatgca gccatctgga tgataaagtg atcgacagcc ggaccgtgcg cagcggaaat
    10861 gccatccgcc ggcggagggt gtgtctgagc tgccagcatc gcttcaccac ctatgaggag
    10921 gttgtcaagt ccacgctgcg ggtgatcaag cgggacggac gccacgaaga actggaccgg
    10981 aaaaaactca tggccggtgt cgtgcgcgca tgcgagaagc gaccgatcag tctccagcaa
    11041 atcgagatga tgctggacag tgtgatgaat gaactcgaag ccgagtatga ccgcgaagtc
    11101 cccagcaccg tgattgggaa gaagattatg acgcatctgg aaaagcttga tgaggtggcc
    11161 tatgtgcgct ttgcatcggt ctatcgccgg ttccgggatg tgaatcagtt tctgagtgaa
    11221 gtggaaggac tcataggtcg cgaatgatcc ctcacacgga aaatcaggcg ctggaggcgc
    11281 tggtcgcata cattgaagag acccttcgca gcacctgctc gggcgcatgg accggagacg
    11341 agtctgtccg ccagattgcc ggcggtgtgg ctgagttctg cctggagcag ttttcttcgc
    11401 tccgcattcc aagcgcctac cttgcgctgc tgacggcccg cgctctgtgg gctgtcggag
    11461 aagaggaagc cagccggctg ttcgtaatgg cgcactgccc gccggacatc cggaagatct
    11521 gcgttccgtt cagccgccgc ccggatgaaa tgaaccgctg ctggaaactc tgcgcagcga
    11581 agattcttcg ctcctcgaga tgggtcagcc gttcagatgg tctgatgtgg acgctcgaca
    11641 tgcgccgcat cgaatggagt tccggtcagg ggcttgaact ggccatgcgg cagggcctgg
    11701 ctgaacttat tcaaccgatt gcgccggtct gggattcctt ctcgggccgc ggcgaactgg
    11761 ggctgcttgg cctgcgggag cagataggtc gtttaatcaa ccgtcctccg cagtctaaaa
    11821 cggtccagcc cgttgcggcc gaaatgagag gcctatgcga agacctgctg atgcgcattg
    11881 gaaaaagccg agggtggcag ggctgcccgg aaattgttga actcagcctt tgacattcct
    11941 gtcaattctg gtaaaaacca gccctcaatt tgcatgtatt gaagaacgag tcaggagtaa
    12001 ggaaatgtcg agacatccaa gtttgaaagc ctccggaaag attgcggcca agcgcaatgt
    12061 gctgaagcgc tatgagcgcg tcactctgct ggaaagccgc gggaaatgga aagagggaga
    12121 ccgtggactt ggactcccca agacaaaacc cgccgaata
//
LOCUS       DAOQWR010000072        12114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5737_length_12114_cov_4.612157, whole genome shotgun sequence.
ACCESSION   DAOQWR010000072 DAOQWR010000000
VERSION     DAOQWR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_12114_cov_4.612157"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4334)
                     /locus_tag="PKK36_07040"
     CDS             complement(<1..4334)
                     /locus_tag="PKK36_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR94349.1"
                     /translation="MRNPLQFCVVLSILLAIGANPPAEAQNLVFSEIMFNPAPGTNIY
                     ASDDYEFIELHNAGAGPVDLTKAYFSKGITYTFSNVTMLAAGQYLVIAKNTNAFTERY
                     PSVTNRAPWFFDGQLANDGEEITLKNTNGITLFSATYSDRIDLADGNGSSLVFAHPAA
                     DPDEPTNWCASARLHGNPGGADTCAEQTLVINEILAHTDWPLQDSVEIFNPGTNAINP
                     SGWYLSDDNAIRNKYRISITNIPAGGYLVISNTQFAAADIPFALSELGESVYLTSADS
                     SSNLAGTVDFHEFEASENGWSFGRYPNGTGTMVTMAALSLGTSNGPPRVGPAVISEIM
                     YNPANDTAEDEYLELLNISGAPLELYDPANPSNTWKLTTAVEFTFPSGLVLAANERLL
                     IVGTTNIPLFVQTHALATNVQIIGAWTGRLNNAGEEVRLYKPGPPETNMVPYYLVENI
                     DYSDDDPWPTAPDGNGPSLERIHSTNYGNTAENWFAGAPGGSPGAAPVAGLINGTVSP
                     PAAGIAFTATVSVVAETMPTQVLFSAEIAGIASNWVMRDDGTAGDLVSGDQIYTAVIP
                     GQADDQWIYYQFHAFSTNDAVISAPASETEYVESPGLTLQIAWQGLRTVVEPGPAWTT
                     YEASGWTSHEVIFDIFLNGAGEVLVDDIVLLDAGLTNHVPNGEGDFEAPLAGWRFKGN
                     HSNSYREVLSAENSNGVLHVVSTGAGASYNDAVGFNMDPPMVSNINNLVHLTFRARQV
                     GREVPQWSWVAVGTPPLEPVINEIMYHSAATNEEDFEYIEIFNPGPDIDLSGARLSGA
                     GFTFENGTLLASNAFLAACKSPSLIAAEYGITNVTGPLGGTLQNSGEEIRLENSYGRV
                     MDGVEYGEKRPWPEAADGYGPSLERRSRLLPSSNAASWAASSVSTNWQTVTWTTEIAS
                     ANSGLKFFLDFDGKVWLDNVSVKPAAGGGEMLPNGSFESGMTGWSATGNHWRSRTEAS
                     AGITGSTALAVAGTFSRFVFPGMSPDLVVNHGDAATNCVSSGPLPATNGHSYVVSMQV
                     RRSGVGGTLHIAAGGAANSVSLGLRGTPGAANSAQQDLPAPLIEDVDKLHDIVDLSQT
                     NVITAEIPSGGIATVTLNYRIFTTNGYQFTDHLYSSLPMTNAGAGIYRVEFPPVTTRW
                     SVVRYHVVATGTNGMVSRIPREDDPQCDYAFWANGHSPQTTLPDWHLFIDGNPVIYPV
                     AARGCAVSPDGQVFLDTEIRHRGGPTTNYSQRGLAMRTHRARPLDTWFADNQGGINFR
                     HRQNNSAFYHRRIVNEPIAYDIQRAIGLPAPRLRHIVMWIDGAPTITTELEDPEESYA
                     EDNGIPLDDLLTRSGNAGRRIIVGDESLDNLWSVVNALDAAAPGDIPALVETHLDYES
                     IRYVLGFFGAIGNIDQNISWNMFQHRSASDGKWRQYPWDTDFGLALDTTFNPAPTNMH
                     PYYATPLHP"
     gene            complement(4404..6122)
                     /locus_tag="PKK36_07045"
     CDS             complement(4404..6122)
                     /locus_tag="PKK36_07045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR94350.1"
                     /translation="MKRTLIAAPAVLLLLLSCARIHGPLNTSVANHPWLTLLLLAGAA
                     SMVRSRRMGDATRRLGHLFFESRDAAFYSAVWVAAVVLYSLAGRIIFQSQPHLDDGVA
                     SLFQARLFARGEIVTGLAPARDFFWQIFVLDDSNGLPHRCGMYPPGWPALLVPGVWLG
                     MPWIINPILGGALAVAAAMLAAEMFNRTTGRIAALLTLMSPYAAVLSATFLSHIATAL
                     LCTLCAWSVFRLVRTGTWHYGAVAGLTWGWAFLCRPLTALVVGTVIALIPLFSFKQAL
                     KAWRAVALALLLAGAAGGIQAGFQKVTTGDASTPGHVLGMGRRGKFGFVKLDWVRTHT
                     PRLGMEHTLDRLRFLNDRLTGWPVGVFVLAAIPLFCRSARWRDAWLLLPVIALAFVYA
                     FYWYYEILFPARYLEAGTPLVLVLAGRGLQIIFEKSPSAKYPAAAVLACLLFALITVP
                     ADYLSAFEPDHGDVEPNLMRTLKKHGVENAVVFYDAVDRDPIYYHAFNDYYATAFLHN
                     DIDLKGDVVFARNLRERNVELFALYPGRRYYLYRYSREKQKPAFYEMIPEEELFEVIT
                     LDELKN"
     gene            6251..6820
                     /locus_tag="PKK36_07050"
     CDS             6251..6820
                     /locus_tag="PKK36_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNR94351.1"
                     /translation="MKTNRIIGTMGIAGAAMMVLWGAGCETIAQQRRRQEMYEQEDKR
                     LVQESMRRIEGRVETLEFELERIQQELGRTQDAAAASARAATESLEPRMGRVESRMAA
                     LEKQREQDRQELVDSLSKKMADIMKTSSSAGRSAKRSGYGYEHTVEAGQTLSEIAAAY
                     GVSVQRILEENSLKNANMLKVGQVLFIPE"
     gene            complement(6828..7856)
                     /gene="rfbB"
                     /locus_tag="PKK36_07055"
     CDS             complement(6828..7856)
                     /gene="rfbB"
                     /locus_tag="PKK36_07055"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNR94352.1"
                     /translation="MKILVTGGAGFIGGNFVRLMLSEYADIEIINLDALTYAGNLESL
                     SDLEGNPRHTFIRGDIASMEDVEKVFSGHAFDAVINFAAESHVDRSLHMGVSHFIETN
                     IKGTQLLLDAARQHGVPRFVQISTDEVYGSLGPQGRFSESSPVAPNNPYAATKAGADF
                     LVRAACRSHKLDAVITRCSNNYGPFQFPEKMIPLMISNILEDKPLPVYGDGMQVRDWI
                     YVEDHCRAVDAVLRRGKTGEVYNIGGLYDVPNIEVVKLLLKLLEKPESLIRYVEDRPG
                     HDRRYAMDAEKIQKELGWKPRVKFEEGIRKTIDWYLANREWLSHVRSGNYMAYYDRMY
                     GKRKAAAG"
     gene            complement(7853..8701)
                     /gene="rfbD"
                     /locus_tag="PKK36_07060"
     CDS             complement(7853..8701)
                     /gene="rfbD"
                     /locus_tag="PKK36_07060"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNR94353.1"
                     /translation="MKIVVLGSRGMLGTDLMTAAREKGHAVLGLDQPAIDIGDYNNIW
                     RHIPPCDWIINCAAYTNVDRAETHPAEAFSVNSEGALNVALVALRKKVRLLHISTDYV
                     FDGHRRRPYSEKIHPNPINVYGASKLAGEKNIRGSGCSYIIVRTQSLFGLHGHNFVKA
                     ILNQISKGERELKVVMDQISSPTYTAHLAQGLLKLLEAPDRQILHLSATGSCSWYDFA
                     RAIVEEVHANAQVIPIPSSDIRRHAKRPAFSVLDNRTCEALTGYTMPTWEQGLREYLK
                     GEKLTS"
     gene            complement(8780..>9627)
                     /locus_tag="PKK36_07065"
     CDS             complement(8780..>9627)
                     /locus_tag="PKK36_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR94354.1"
                     /translation="GLEVSCIAGTSAGALVGAVYASGRLREFEDLSEHMDWKKVLYYF
                     SDISFARAGLVDGEKVCKMLRKYTTARNIEDLPLPYGAVTADILTGEEVVLDRGEIID
                     AVRASIAFPGIFTPVKIGNRLLMDGGIVNPVPVNVVRDMGADFVIAVDLNHDRMSPEA
                     RSLRKEKHTAEKPGAGETELPEPLRKLSGKLKEMVADFKMQRSSAPHIFDILGNASRI
                     IEGRLSEAMLQADPPDVLIRPKVGHVSFMEFNRSVSTISAGYEAAREPLRKAARRIRG
                     LPPRH"
     assembly_gap    9628..9727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9728..9885)
                     /locus_tag="PKK36_07070"
     CDS             complement(<9728..9885)
                     /locus_tag="PKK36_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR94355.1"
                     /translation="MKEILKRKCRIGLALGGGAARGWAHVGVLQALEEAGLEVSCIAG
                     TSAGALVGA"
     gene            complement(9998..10558)
                     /locus_tag="PKK36_07075"
     CDS             complement(9998..10558)
                     /locus_tag="PKK36_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94356.1"
                     /translation="MHWRPYAVHYAKRLLICLLFIAVLFPGAYGYDWSTYNVEDFEEP
                     AGTIGCFEFNPADDIYGVSVGDGTWLTGTPVFGDFFLPVFYNGIEDAVYAGVGMTLRI
                     MPHWKFAPFAGGGASYNLSLSGAEDSEFSENPRGESYWGGHAEGGVRLQTSAGFCEIL
                     GRHTWSSSEIEDADYWTVRIGWGNNF"
     gene            complement(10617..10958)
                     /locus_tag="PKK36_07080"
     CDS             complement(10617..10958)
                     /locus_tag="PKK36_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HNR94357.1"
                     /translation="MPSIRVQISEAPFDAKKLLPELSRLTAESLGKPEQYVMAVAEPA
                     AILMSREPGPAAMAEVRSIGGLNASKNTRLTQALCALLKSELGIPPERVYVTFVDVKG
                     ENWGHDNSIFG"
     gene            complement(10974..>12114)
                     /locus_tag="PKK36_07085"
     CDS             complement(10974..>12114)
                     /locus_tag="PKK36_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR94358.1"
                     /translation="EAVDEVSAFLKFWILEVLESEAGGSGQRADIQSVIEGMDFGYIT
                     RLQTNIMHAYIQDPASQPAPDGVTEEGMVYIPGGYFLMGRADATYANDTFPLHIVFVS
                     PFLLDMYETDNAGYREFADHVKNTGDSSMAHPDAPPLKDHSAEGWKDRNLSGDRKPVI
                     GIDWFDAYAYAKYKDKRLPTEAEWEFAARGRDGRIFSWGDEPPKTRILSTPFGRDYVA
                     GEIDRLSRPAQRPGTERKGLMGRKPEEQAVQRTTLPAGTWDVDKGLPQEAIDSGYFDE
                     NKDILSPFGVFHMTGNAAEWTQDWYDPHYYRTSPTSNPPGPDKGSSRVFRGGSYLSAS
                     VNELETTWRGFYPPNMRGRQGMSSNGKPMIGVRCAKSIPGVDAGL"
BASE COUNT         2398 a         3458 c         3689 g         2469 t
ORIGIN      
        1 ggatgcagcg gcgtggcgta atacggatgc atgttggtcg gggccggatt gaaggtggtg
       61 tccagggcca gtccgaaatc cgtatcccag ggatactgcc gccattttcc gtcgctggcg
      121 gaccggtgct ggaacatatt ccacgagata ttctggtcaa tatttccgat tgccccgaag
      181 aagccaagta catagcggat ggattcataa tcgagatgag tttcgaccag tgcgggaatg
      241 tccccgggtg ccgccgcatc cagcgcgttg accacactcc acaaattgtc cagagattcg
      301 tcgcccacta tgatccttcg gcccgcattg ccggaccgtg tcagcagatc atccagcgga
      361 atgccgttgt cctccgcgta tgattcctcc ggatcctcca gctcggtggt gatcgtgggg
      421 gccccgtcaa tccacatcac gatgtgccgc agccgcgggg cgggcagtcc gatggcgcgc
      481 tgaatgtcgt aggcgatggg ttcattgaca atgcggcggt gatagaaggc gctgttgttc
      541 tgccggtgcc tgaaattgat tccgccctga ttatccgcaa accaggtgtc cagcgggcgc
      601 gcgcgatgtg ttcgcatggc aaggccccgc tgcgagtagt ttgtggtggg gccgccccgg
      661 tgccggattt ccgtgtcgag gaatacctgg ccgtcggggc ttaccgcgca gccccttgcc
      721 gcaacaggat agatgacggg attcccgtca atgaacagat gccagtccgg caaagtggtc
      781 tgcgggctgt ggccgttggc ccagaaggca tagtcgcact gcgggtcatc ttcgcgcggg
      841 atgcgcgaaa ccatgccgtt ggttcccgtt gcgacaacgt gataccggac caccgaccat
      901 ctggtggtca ccggcggaaa ctcgaccctg tagattcctg cacccgcatt ggtcatgggc
      961 agggaggaat aaaggtggtc ggtgaactgg tagccgttgg ttgtgaaaat gcggtagttc
     1021 agggtgacgg ttgcgattcc tccggatgga atctccgccg tgatgacatt ggtttgggag
     1081 agatcaacga tgtcatgcag tttgtccaca tcctcaatca gcggcgcggg cagatcctgc
     1141 tgcgcggaat tcgcggcgcc cggtgtgccg cgcaatccca gcgaaacact gttggcggcg
     1201 ccgccggcgg cgatatgcag cgtgcccccg actccgcttc ggcgcacctg catggaaacc
     1261 acatagctgt gcccgtttgt ggccggcagc ggccctgaac tgacgcagtt tgtggcggca
     1321 tccccgtggt tgacgacaag atccggagac atgcccggga acacaaaacg ggagaacgtc
     1381 ccggccacgg ccagcgctgt gcttccggtt atgccggcgg aggcttctgt ccgtgagcgc
     1441 cagtggttgc cggtcgcgct ccagccggtc atgccggatt caaaactccc gttcgggagc
     1501 atttcaccgc cgcccgcggc gggcttgacc gaaacattgt ccagccagac cttgccgtcg
     1561 aaatccagga agaatttcag cccggaattg gcggaggcaa tttcagtggt ccatgtcacg
     1621 gtctgccagt ttgtggaaac ggaggaggcc gcccagctgg ccgcattgga ggaggggagc
     1681 aggcggctgc gccgttccag cgacgggccg tagccgtcgg ccgcttcggg ccacgggcgc
     1741 ttttctccgt actcgacccc atccattacg cggccatagc tgttttcaag acggatttcc
     1801 tccccgctgt tctgaagcgt tccgccaaga ggtccggtta cgttggtgat gccgtattcc
     1861 gccgcgatca gagagggcga cttgcaggcc gccagaaacg cattggatgc aagaagggtt
     1921 ccgttttcga atgtgaatcc cgcgccgctc aggcgcgcgc ccgaaaggtc aatatccggg
     1981 ccagggttga agatttcaat gtactcgaaa tcttcttcat tggtggcggc agagtggtac
     2041 atgatttcat tgatcaccgg ttcaagcggc ggtgttccga cggccaccca gctccattgg
     2101 ggaacttccc ggccgacctg ccgcgcgcgg aaggtcaggt gcaccagatt gttgatattg
     2161 gaaaccatcg gcgggtccat gttgaatcca accgcgtcgt tgtagcttgc ccctgcgccg
     2221 gtggacacca catgaagaac cccgttgctg ttttctgcgc tgaggacttc gcggtatgaa
     2281 ttgctgtgat tccccttgaa tctccatccg gccagggggg cttcgaaatc gccctcgccg
     2341 ttcggcacat ggttcgtcag tcccgcatcg agcagcacaa tatcatccac aagcacctcg
     2401 ccggcaccgt tcaggaagat gtcaaagatc acttcatgcg aggtccagcc ggaggcttca
     2461 taagtggtcc aggcgggtcc gggttccaca accgttctca ggccctgcca tgcaatttga
     2521 agcgtcagcc cgggggattc cacatattcc gtttcagaag ccggtgcgct gatgacggca
     2581 tcattagttg agaaggcgtg aaactgatag tatatccact ggtcgtctgc ctgccccgga
     2641 atgacggcgg tatagatctg gtctccggaa acaagatctc cggccgttcc gtcatcccgc
     2701 atgacccagt tgctggcgat gcccgcgatc tcggcgctga acagaacctg tgtgggcatg
     2761 gtttccgcga caacggaaac ggtggcggtg aaggcgatgc cggcggcggg cggcgaaacg
     2821 gttccgttga tgagacccgc gaccggcgcc gcgcccggcg atccgccggg cgcgccggca
     2881 aaccagtttt ccgccgtgtt tccgtagttc gtgctgtgga ttctttccag cgaaggcccg
     2941 ttgccgtcgg gtgcggtcgg ccagggatcg tcatcactgt aatcaatgtt ttcgaccaga
     3001 taatagggca ccatgttggt ttcgggcggg ccgggcttgt agaggcgcac ctcctcgccc
     3061 gcgttattca atcgtcccgt ccaggccccg atgatctgga cattggtggc gagtgcgtgg
     3121 gtctggacga acagggggat attggttgtg ccgacgatca gcagccgttc gttggcggcg
     3181 agaacaagac cggacgggaa cgtgaattcg actgctgtgg tcagtttcca tgtattggat
     3241 ggattggcgg ggtcgtacag ctccagcggt gctccggaga tgttcagcag ctcaaggtat
     3301 tcatcctccg cggtgtcgtt ggccggatta tacatgattt cgctgatgac cgcgggcccc
     3361 acgcgcggcg gcccgttgga ggttccgagg ctgagcgccg ccatggtgac cattgtccct
     3421 gttccgttcg gatagcgacc gaacgaccag ccgttttccg aggcttcaaa ctcatggaaa
     3481 tctaccgttc cggcgagatt gcttgatgag tcggccgagg tcaaataaac gctctcgccc
     3541 agttcgctca gggcgaaggg aatgtccgcg gccgcaaact gggtgttgga aatcaccaga
     3601 tatccgccgg caggaatatt cgtgatgctg atcctgtatt tgttccggat ggcattgtcg
     3661 tcgctgagat accagccgga aggattgatg gcgttcgtgc ccggattgaa aatttcaacg
     3721 gaatcctgca gcggccagtc ggtatgcgca aggatctcat tgatgacaag ggtttgttcc
     3781 gcgcaggtgt ccgccccgcc cggattgccg tgcaaacgcg cgctcgcgca ccagttggtc
     3841 ggctcgtcgg ggtcggccgc gggatgggcg aagaccagcg aagagccgtt gccgtccgcc
     3901 aggtcaatgc ggtcgctgta agttgccgag aacaaagtga tgccattggt gttcttcagc
     3961 gtaatttcct cgccgtcgtt ggcgagctgg ccgtcgaaaa accacggggc ccggttggtc
     4021 acggagggat agcgctccgt gaaggcgttg gtgtttttgg caatcaccag atactggccc
     4081 gctgccagca tggtgacgtt ggaaaaagta taggtgatgc cttttgagaa ataggctttg
     4141 gtcagatcaa ccggcccggc gcccgcgttg tgcagttcga tgaactcgta atcatcggag
     4201 gcataaatat ttgtcccggg ggccgggttg aacatgattt cgctgaacac cagattctgg
     4261 gcttccgctg gcgggtttgc gccaatggcg aggagtatgc tcagaacgac gcaaaactgt
     4321 aacggattcc tcatatatat gtcccctgaa ttcagggtat attaaatata atccgtaaaa
     4381 cagaaaatta aagcggggaa gcgctaattt ttcagttcat ccagcgtaat gacttcaaaa
     4441 agctcttctt ccgggatcat ttcatagaaa gcaggctttt gtttttcccg gctgtagcga
     4501 tagagatagt agcggcggcc cggatacagc gcaaacagct cgacgttgcg ttcccggaga
     4561 ttccgggcaa agacgacatc ccccttgagg tcaatgtcgt tatgaaggaa cgccgtggcg
     4621 taatagtcat tgaaagcatg atagtaaatg gggtcgcggt ccaccgcgtc atagaacacc
     4681 accgcatttt caacgccgtg ctttttcagg gtgcgcatca gattgggctc cacatcgccg
     4741 tgatccggct caaaagcgct cagatagtct gccggaacgg tgatcagggc aaagaggagg
     4801 caggcgagca cggctgcggc gggatatttg gctgacggag acttctcgaa aatgatctgg
     4861 aggccccgtc ccgcaagaac aaggaccagg ggggtgccgg cctccagata acgggccggg
     4921 aacaggattt catagtacca gtaaaaggca tagacgaagg ccagggcgat gactggaaga
     4981 aggagccatg catcccgcca gcgggcgctg cggcagaaca ggggaatggc cgcaagaaca
     5041 aaaacgccga cgggccagcc cgtcaggcga tcattcagaa aacgcagccg gtcgagcgtg
     5101 tgctccatgc caagccgggg tgtatgtgtc cggacccagt ccagtttcac gaacccgaat
     5161 ttgcctcttc gacccatgcc cagcacatgg ccgggagtgg atgcatcacc ggttgtgact
     5221 ttctgaaatc ccgcctggat gcctcccgcc gcaccggcca gcagcagcgc cagcgcgacg
     5281 gctcgccatg ccttcagggc ctgtttgaag ctgaacaggg gaatgagcgc gatgacagtc
     5341 ccgaccacca gcgccgtgag cgggcggcac aggaacgccc agccccaggt caggccggcg
     5401 actgcgccgt aatgccaggt tccggtccgc accagccgga atacgctcca ggcgcacagt
     5461 gtgcagagaa gagcggtggc aatgtgcgag agaaacgtgg cggacagaac ggccgcatag
     5521 ggggacatca gggtcagcag agcggcgatg cggcccgtgg tgcggttgaa catctccgct
     5581 gccagcatgg cggcggccac cgccagcgcc ccgcccagaa tcggattgat gatccacggc
     5641 atgcccagcc agactccggg aaccagcagg gccggccagc ccggcggata catcccgcag
     5701 cggtgcggca ggccgttgga gtcgtccagc acgaatatct gccagaagaa atcccgtgcc
     5761 ggcgcaagac cggtgacaat ttcacctcgc gcgaaaagcc gggcctggaa aagcgacgcc
     5821 accccgtcat cgagatgcgg ctgggactga aagataatcc ggccggcaag gctgtagaga
     5881 acaacggccg cgacccagac ggcggaataa aacgccgcgt cacggctctc aaaaaacaga
     5941 tggccaagcc tgcgggtcgc atctcccatc cggcggctgc ggaccatgga ggccgcgccg
     6001 gcgagcaaga ggagtgtcag ccacggatgg tttgcgacag aggtgttcag cggcccgtga
     6061 attcgtgcgc acgaaagcag gagcagcaaa acggccggcg cggcgatcag tgttctcttc
     6121 aaggtcacct ggtccttttt ggttgaaaca aaaagcgctt tgtaggtgca gggggaggga
     6181 aagtcaacgc tgatatctgg cctgcatatt ttcttgagtt tgcgcaggcc ttggggtaag
     6241 cagaggcagt atgaaaacga atagaataat cgggacaatg ggaattgcgg gcgcggccat
     6301 gatggttctc tggggcgcag gctgcgagac gattgcgcag cagcgccgcc gccaggaaat
     6361 gtacgagcag gaggacaagc ggcttgtgca ggaatccatg cggcgcatcg agggtcgcgt
     6421 cgaaacgctg gaatttgaac tggaacgcat ccagcaggaa ttgggccgca cgcaggacgc
     6481 ggcggcggcc tccgcccgcg ccgccaccga gtccctcgag ccccgcatgg gcagggtgga
     6541 aagccggatg gcggctcttg agaagcagcg cgagcaggac cgccaggaac ttgtggactc
     6601 gctgagcaaa aagatggcgg acatcatgaa gacttcatcg tccgccggcc gcagcgcaaa
     6661 acgaagcgga tacggctacg agcacacggt ggaggccgga cagacgcttt ccgaaattgc
     6721 agcggcctat ggcgtaagcg tccagcgcat cctcgaggaa aacagcctga agaacgccaa
     6781 catgctgaaa gtcggccagg tgctctttat tccggaatga cggaacttca cccggcagcg
     6841 gcttttcttt tcccgtacat ccggtcgtag taggccatgt agtttccgct tcgcacgtgc
     6901 gagagccatt cgcggttggc gaggtaccag tcaatggttt tgcggatgcc ctcttcaaac
     6961 ttcacgcggg gcttccagcc cagttccttc tggattttct ccgcatccat cgcatagcgc
     7021 cgatcgtgtc cgggacggtc ttcaacgtac cggatcagcg attcgggctt ttccagaagt
     7081 ttgagcagca gtttgacgac ttcgatattg ggaacatcgt agaggccgcc gatgttgtag
     7141 acctctccgg ttttgccccg gcggagcacg gcatccacgg cccggcagtg atcttcgaca
     7201 taaatccagt cgcgcacctg cattccgtcg ccgtagacgg gcaggggttt gtcctcgagg
     7261 atgtttgaga tcatcagcgg gatcattttt tcaggaaact ggaagggtcc gtagttgttg
     7321 gagcagcggg tgatcacggc atccagcttg tgcgaccggc aggcggcccg cacgaggaaa
     7381 tccgcgcccg ccttggtggc ggcataggga ttgttcggcg cgacggggct ggattccgaa
     7441 aaccggccct gcggcccgag gctcccgtac acttcgtccg tcgagatctg cacaaaccgg
     7501 ggaacaccgt gctgccgcgc cgcatccagc agaagctggg tgcccttgat gttcgtctca
     7561 atgaaatgtg aaacgcccat gtgcagactc cggtccacat ggctttcggc cgcaaaattg
     7621 atgacggcgt caaacgcatg tccgctgaag accttctcga catcttccat ggatgcaata
     7681 tcgccgcgga tgaaggtgtg gcggggattg ccttctagat cggaaagact ttccagattg
     7741 cccgcatagg tcagcgcgtc cagattgatg atttcgatat ccgcatattc gctcagcatc
     7801 agacggacga aattgccgcc gatgaaaccg gcgcctccgg tcacaaggat cttcatgatg
     7861 tcagtttctc tcctttcagg tattcgcgca gtccctgttc ccaggtgggc atggtgtatc
     7921 cggtgagcgc ctcgcaggtc cggttgtcca gcacggaaaa ggccggtctt ttcgcgtggc
     7981 gccggatgtc cgaggaggga atcggaatca cctgtgcatt ggcgtgaacc tcctccacga
     8041 tggctcgcgc gaagtcatac cacgaacagg agccggttgc ggacaggtgc agtatctgcc
     8101 gatcgggcgc ctccagcagt ttcagcaggc cctgcgccag atgagccgta taggtggggg
     8161 aggaaatctg gtccatcacc actttgagct ctctttcccc ttttgagatc tgattgagga
     8221 tggccttcac gaaattgtgc ccgtgcagac cgaagagcga ctgggtgcga acgatgatgt
     8281 aggaacagcc ggacccgcgg atgttttttt cgccggcgag tttgctggcg ccgtacacgt
     8341 tgatggggtt gggatggatt ttttcggaat agggtctccg ccggtgcccg tcgaacacgt
     8401 agtccgtgct gatgtgcagc aggcggacct tcttgcggag cgccacgagg gcgacgttga
     8461 gcgctccttc ggaattgacg gaaaaggctt cggccggatg tgtttccgcc cggtccacat
     8521 tggtgtaggc cgcgcagttg atgatccagt cgcagggcgg gatgtgccgc cagatattgt
     8581 tgtagtcgcc gatatcaatg gccggctggt caaggcccag cacggcatgt cccttctcgc
     8641 gggcggcggt catcagatcc gtgcccagca ttcctcttga tccaagaaca acaattttca
     8701 tggcagcagt ttcatccaag aacaatcaac agaaaggcag tctatagccg gtcgggtgct
     8761 ttgcaaagcc gattttaact cagtgccgcg gcggaaggcc ccggatgcgc cgggcggcct
     8821 tccgcagggg ttcccgcgcc gcctcataac ccgcggaaat cgtgctgacc gaacggttga
     8881 actccatgaa gctgacgtga cccaccttcg gccggatcag gacatccgga ggatcggcct
     8941 gaagcatggc ttctgaaagg cgcccctcta tgatacgcga ggcattccca aggatatcaa
     9001 agatgtgcgg ggcggagctg cgctgcattt tgaagtccgc caccatttcc ttcagcttcc
     9061 ctgacaactt gcgcagcggt tcgggcagtt cggtttcgcc ggctcccggc ttctccgcag
     9121 tatgcttctc tttgcgcagc gaacgggctt caggactcat gcggtcatga ttgagatcca
     9181 ccgcgatcac gaaatcggcc cccatgtcgc gcacgacgtt cacgggcacg ggattcacaa
     9241 tgcccccgtc catcagaagc cggttcccga ttttgacggg agtgaatatc ccgggaaatg
     9301 caatgctggc gcggacggcg tcgatgattt caccgcggtc gagcacaact tcctccccgg
     9361 tcaggatgtc tgcggtcacg gccccgtagg gcaggggcag gtcttcgata ttccgggcgg
     9421 tggtgtattt ccggagcatt ttgcacacct tttcgccatc caccagtccg gcgcgcgcga
     9481 aagaaatgtc tgagaaataa tagagcactt tcttccagtc catatgttcg gacaaatcct
     9541 cgaattcacg cagccgcccg gaggcataaa cggcgccgac cagcgccccg gcgctggtgc
     9601 ccgcgatgca ggagacttcc agcccggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnngcg ccgaccagcg ccccggcgct ggtgcccgcg atgcaggaga cttccagccc
     9781 ggcttcttcc aaagcctgaa gcaccccgac atgggcccat ccgcgggccg ctccgccgcc
     9841 cagcgccagg ccgatccggc attttctttt cagtatttct ttcattgcgt ccccaaattc
     9901 aatttcgcgg ttgcgacgga gcgcgaccct ccagaaaacc gataatccct gaccttgata
     9961 tgggagggtt ttgctccgtc aaaaccgctc ctgatcatta aaaattattg ccccagccga
    10021 tgcggacagt ccagtagtcg gcgtcttcga tttccgatga agaccatgtg tgccgtccga
    10081 ggatttcgca gaatcccgcc gaagtctgca gtcgcacccc gccctcggca tgtccgcccc
    10141 aatatgactc gcccctgggg ttttcgctga actcactgtc ctccgcgccc gagagcgaga
    10201 gattgtagct ggcgcctccg ccggcaaacg gcgcgaactt ccagtggggc atgatgcgaa
    10261 gcgtcatacc aaccccagca tagacggcat cttcgatgcc gttgtaaaaa accggaagga
    10321 aaaagtcccc gaaaaccgga gtgccggtca gccaggtgcc gtcaccgaca ctcaccccgt
    10381 agatatcgtc cgccggattg aattcgaagc agccgatagt cccggccggc tcctcaaaat
    10441 cctccacgtt atacgtgctc cagtcgtatc cgtatgcccc ggggaataag actgcgatga
    10501 aaagcaggca aatcaataat cgtttggcat aatgaaccgc gtatggacgc caatgcatgc
    10561 gaatgagttt cccggtttca tttgcgttga ttcgcgtgca ttcgtggttc atcacatcat
    10621 ccgaagatgc tgttgtcgtg gccccagttt tcgcctttca cgtccacaaa agtgacatac
    10681 acgcgctccg gcgggatgcc gagttccgat ttcagcaggg cgcacagcgc ctgcgtcagc
    10741 cgggtgtttt tggaggcatt cagtccgccg atgctgcgga cctcggccat ggcggcgggt
    10801 cccggctcgc gcgacatcag gatggcggcg ggctcggcga cggccatgac atactgctcc
    10861 ggcttgccca gtgattctgc ggtcagccgc gacagttccg ggagcagttt tttcgcgtca
    10921 aacggagctt cggatatctg cactctgatg gatggcattt tgaacctccg gatttataaa
    10981 cccgcgtcca cgcccgggat ggattttgcg cagcgcacgc caatcatggg ttttccgttt
    11041 gaactcatgc cctgccgccc gcgcatgttc gggggataga atccccgcca ggtcgtttca
    11101 agctcgttga cgcttgcgct gagataggag ccgccgcgga acacgcggga cgagcctttg
    11161 tcgggtccgg gcggattgct ggtcgggctg gtgcggtagt aatgggggtc ataccagtcc
    11221 tgtgtccact cggccgcgtt gccggtcatg tggaacaccc cgaacgggct caagatgtct
    11281 ttgttctcat caaagtatcc ggaatcaatg gcttcctgcg ggagcccctt gtccacatcc
    11341 caagttccgg cgggaagtgt ggtgcgctga acggcctgct cttcgggctt gcgtcccatc
    11401 aggccctttc tttcggttcc cggccgctgg gcgggacgtg acaggcggtc tatttcgccg
    11461 gccacataat cacggccgaa cggcgtgctc aggatccggg ttttgggcgg ctcatcgccc
    11521 catgagaaaa tccggccgtc gcgtccgcgc gcggcaaatt cccattcggc ttcggtcgga
    11581 agccgtttgt ctttgtattt ggcataggcg taggcgtcga accagtcaat gccgatcacc
    11641 ggtttgcggt ctccgctgag attgcggtcc ttccagcctt cggccgagtg gtccttgagc
    11701 ggcggcgcgt cgggatgagc catggaggag tcgccggtgt ttttcacatg gtcggcaaac
    11761 tcgcggtagc cggcattgtc ggtttcatac atgtccagca gaaaggggct cacgaaaaca
    11821 atgtgcaggg ggaatgtgtc attggcatac gtggcatccg cccggcccat caggaaatag
    11881 ccgccgggga tatagaccat gccttcctcc gtcacgccgt ccggggccgg ctggctggcg
    11941 ggatcctgaa tataggcgtg catgatgttc gtctgcaggc gcgtgatata tccgaaatcc
    12001 atgccttcga tcacgctttg aatgtcggcc cgctgtccgg atccgcccgc ttcgctttcg
    12061 aggacctcca gaatccagaa cttcagaaac gccgacacct catccacggc ttcg
//
LOCUS       DAOQWR010000073        12064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5765_length_12064_cov_4.871763, whole genome shotgun sequence.
ACCESSION   DAOQWR010000073 DAOQWR010000000
VERSION     DAOQWR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_12064_cov_4.871763"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PKK36_07090"
     CDS             complement(<1..224)
                     /locus_tag="PKK36_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94359.1"
                     /translation="MIRFEVCIAHYGHIGFRACGTDLVSICIHSRKLLRPYSPDEIRL
                     LITEIDVGGPLVPGRIVARVAGAVAGAVKR"
     gene            477..6386
                     /locus_tag="PKK36_07095"
     CDS             477..6386
                     /locus_tag="PKK36_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94360.1"
                     /translation="MIECSWVADGADNTDWWEAVVPPMSDGTVLRYKVGSFKTQNDFN
                     GWDVPFPNSTGDIERKTKMMGEWEIAGFDPSTVVYRPHNDFGLTATGLVDGFHFLQAR
                     AFLQRDGKAAIYNTFPQTFYLDTETPRGEIKYPTAGETLWQNEYGAVVRTDPTVREVW
                     YHIEDGNAANDDGQTGNDYGNGTNALGEAAWMQAYSVTPSLSIDSEYPLEWRFSYHNI
                     PTNGSGVIYVRLLEISSSTNFSLNPVDGHCTELVRTNSTQAPAVTFRFNWPDADGFTL
                     QEGWTIKLDFSTSLGVPGDESRFLIRINDVAQSRDDYRIDWGTGRLEFDMPDLYNGDS
                     NFLHHIAITYEMYGGVVLQAHRYIKFQSTASGPLVQIVDPPEVDSDGQPFKIVLPDVA
                     SPSPTQRQYTIQVETDLAARDVWIAFTNSAGEAVKLASVTNDLTGTVSAVTGSTLLTG
                     TGTLFNSQLSVGSTLLLGTNFLNVTQIVSGTNLYLSSPWTEASASGMTAYRVDGNPAV
                     SGNKQIWRFLWTNMTAGYFSFRANVDTNGNNATVEAYASRNVTVLFREMAGSSTNDYD
                     DDDDGIYDSNEAAYTNLPSSNPETWLNGDVHIWMIYGRTDPLLPDTDGDGLPDGLESG
                     WRAPIDPGQTDTNADTNGDGYPNFLADLDPPFFNTVPDNWDVPNYNFNGLRTEKIAGS
                     MTDPANPDSDYDGIPDGIEDWNRNGWVDGDGDPLYPGQDKSTRGSWPDGQRDSFWAET
                     DPNNSDTDGDDLTDGYGEDKDSNGWIAGDINSNRIWETGELWLETDPLNPDTDGDGLP
                     DGWEVRYGLDPWDCGIVGRTNMQTGAIITNILNGAAGNPDGDTIIVGLSTNEYTNLLE
                     YQNGTNPRQPDTGEPPPEGAVVIGRGEEIGSLTGSGAHYQEFTDWTVEDCLVLDEYEG
                     GGNNNQGGDVFKGWDGFDESRDIIAFYARDGGPLASGGDDKFYFRVDFYDLKPLAEEG
                     NLDLYVVVDTGNPASGEMNLPDDVDTITSNRWEAIVAVYQSGQGRVYVDTAQGNNSTT
                     WGQDLSSFGVEARDQNSADGFIDAYYNSELDAVEFAISRQALLDAGWNGLNPDDLNYQ
                     VFTTKDGTCNGCGEGGNPGGGDIGGRSDVRDAIWNDFIAEDYWESQAGLDSILKYWIP
                     GSTRAGQTKVAVVVHGNQAISPGSEIMNLVNDNAGAGYNRPLAVHDLYKAPLNLHITP
                     TLASAIQWASVDTNAGKPWLDGPALNSYIHDLVATNVIYLLGSTFSDHILPYFTKEFN
                     ADNVALANDYLSDIYGFTPSSNTVFWTPERVLDADVFDKIRAMGYSYTILDQGTHLWH
                     WLGRTTALGEYGYRINKINDVNCFVINDLPTGYRFTNFDGGLPAALRSLFVRKGLSGT
                     QDQVVTILSNWEDFGDLDDANAYDINLRWMANHPWIRIVALEEIAAGQVDINNDGWGD
                     AWWAEDRGAAATSKVAHNWLHHATRSDYDNWYLGDGLAEGLQNKIFDIRPGAAMPAAY
                     GMMYTAGIVTDTWDQVTAIADPNLAHLARSVLHASVFQTAFHNSSEDDLSRWSTGEYI
                     YPATQSNSLAGFAKMAQAQTRTAAIFAYADDWAASAGSLTTTVVAQADVDLDGENEYV
                     LANKRLLGIFERIGGRMIAAWVRSSSGHVYQVAGNQAGYSASETEEEGISNVETNGTV
                     VAHRTSCFKDWWAGTSDYVNDLYTFTATNNGWVIRSADSNITKMITLAPGTNQFEAQY
                     TVDGSLNGGQLYVRHGLSPNLYNLLLRGQSTLGYEEHGGGIMTLANNGDGVTISARIG
                     YSDTGHTAAFNSSAVDDSPGGGVEFNTVNMRNQVQTHQVEIYGTGAFSFSLGFAAEAG
                     LSAWEQFCADYGYNPEDYTADADGDLIPNWGEFVSNTNPENSNDFFRVENAAPVPGLQ
                     LSFDTATGRVYYIWYSNSGLINPAWSNATPAGITGQGGSTNWTDDGSQTAPPPGEVPD
                     RFYKIEVEWPE"
     gene            6523..7353
                     /locus_tag="PKK36_07100"
     CDS             6523..7353
                     /locus_tag="PKK36_07100"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNR94361.1"
                     /translation="MIAWPVLGAPVRSGPVEAELVPEVENIAPGQPFTVALRLKMDPP
                     WHTYWINPGDSGLAPRLDWSLPEGFSADELLFPLPAAIPTPPFMTYGFGNEVWLLSTI
                     TPPAVLKSDRVVLKAVVSWLICNEVCVPGSASLVLRLPVQAGGAKPHPVHAAQISAAR
                     ARLPEPAAGWQFRVRHENGLYHLAVFPPPGYAGEVQSASFFPFQNDIIQHSAPQKWRR
                     SAEGYTLELVPAAPAASPESLSGVLVIDERDSRRALRAEAVFPRTETPRDLNKERNTP
                     "
     gene            7350..7958
                     /locus_tag="PKK36_07105"
     CDS             7350..7958
                     /locus_tag="PKK36_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNR94362.1"
                     /translation="MKKKIILTALAVLALFGTAFAPAQVSVGSPAPGFTLPDAQGVSR
                     SLSEFSGKVVILEWTNPDCPFVKKHYDSKNMQALQEKYIARGAVWLSICSSAPGKQGH
                     YSAAEWNDILGRQGSRATALLLDPEGRAGLAYAAKTTPHMFVIDAEGTLVYDGAIDDQ
                     PSTRTDDIPGAKALLADAADAALAGQPVPVPKTAPYGCSVKY"
     gene            complement(7974..9281)
                     /locus_tag="PKK36_07110"
     CDS             complement(7974..9281)
                     /locus_tag="PKK36_07110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNR94363.1"
                     /translation="MASENKMEIATLGGCRFRSPLSLSTVPGDGIGDFIPDTARVRYQ
                     VEFNTPYDNPDILFEKAGPREKIFFDAPKVRAAIVSCGGLCPGINDVIRSICFELKQN
                     YHAKEVLGIRYGYQGLNPAAGRPPLVLTEEAVDGIHLQGGTVLGSSRGAQPVEVMTDF
                     LESRDIDVLFCIGGDGTQRGAHAIAGEVTRRGLKKAIVGIPKTIDNDIPYVQQTFGYL
                     TSIERAHEVLNCAHIEAKAAINGIGLVKVMGRDSGFIAAGASLASQDVDFTLIPEVKF
                     ALGGENGLLSHLERRLARKPHALIVVAEGAGQELMPKDECRRDASGNILHNDIGPFLL
                     AEIKKYFREKNIEANPKYIDPSYIIRSVPANCADSIRCDQFARHAVHAAMAGKTDVLI
                     GLWNDRFIHVPLAEATCCRRYVQPESELWESVISVTGQPRIME"
     gene            9425..9853
                     /locus_tag="PKK36_07115"
     CDS             9425..9853
                     /locus_tag="PKK36_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR94364.1"
                     /translation="MTNKVFLQWCQIRRKVGGLTGHSLDSNVPDIWTPNADVFESADG
                     VIIRMELAGVPLDRLEITCEGRVLNISGVRCESFGAETLAGFRFRQLEIEYGPFHRSV
                     TLPYETDPAHTRATMKNGMLEIRVRAPRAAKKTISKTGKR"
     gene            9857..>12064
                     /gene="lon"
                     /locus_tag="PKK36_07120"
     CDS             9857..>12064
                     /gene="lon"
                     /locus_tag="PKK36_07120"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNR94365.1"
                     /translation="MADQKKNVRSTLPANSDMPGRDAPVVLPVLPLNDLVLFPTMIIP
                     LAVSTQRSIRLIDDVVAGSRHFLAVLQRDRKLDDDKVEPPDLHEFGCLARVLKMIKFP
                     DETVRVLVQGIVRCQIRSYLPENGYLKARYTLLQDEMEDSIELDALARNASQRFQDII
                     TMSPAMPEELKVAAVNMADAGKLADLIASNVNIPLEDRQRLLEEYRPRRRLEKLTTQL
                     NRELEILQLGTQIQSKVSETLSKSQRDYYLREQLKAIQSELGEDNPQQTEMKEISGKI
                     KAAKLPPDVAEIADKERKRLESIPSASPEYAVIRTYLDWLTELPWAVETVDRLDIAAA
                     KKVLDADHYDLARIKDRILEYLSVLKLKKDMKGPILCFAGPPGVGKTSLGKSIARALG
                     RRFIRMSLGGVHDEAEIRGHRRTYIGALPGRIIQGLKRAGTRNPVFMLDEIDKVGADF
                     RGDPSSALLEVLDPEQNYSFSDHYLNVPFDLSHVLFITTANTMDTIPPPLRDRMEVLE
                     LSGYTMNEKLHIAQKFLIRKEIAAHGLKSGQIAFEKAALEKIISDYTREAGVRNLERE
                     VANVCRKVARSVAEGKKDRTVITPQRISQMLGPEKYESEVAEARTEPGIVTGLAWTPV
                     GGRILFIEATQMSGKGQLILTGSLGNIMKESARAALSYVQANSKKLKIEVPEESGKDI
                     HIHVPAGAIPKDGPSAGVAMTMALITLMTGRPLPADIAMTGEITLRGKVMPVGG"
BASE COUNT         2619 a         3388 c         3629 g         2428 t
ORIGIN      
        1 tcccgcttaa cagcgccagc cacggctccg gcgacccgcg cgactatccg cccgggaacg
       61 aggggtccac cgacgtctat ctcggttatg agcaggcgga tttcatccgg cgagtatggt
      121 ctgagaagtt tgcggctgtg aatacaaatc gaaaccagat cggttccgca ggcgcggaaa
      181 cctatgtggc cgtaatgggc gatgcaaact tcgaaacgaa tcattcatcg gccgtcaacg
      241 cgtcatatgc taatactgtc gaatggatct ggcattcgcc gacggagatg caggacggac
      301 cccgcactgg taatgcccag ttttggccgc cgttgtcaaa tgccgctaat tcgacactct
      361 acatcggcat caaaacccca aaggtcgcca gtaatgcggt atatttttat tacacaacca
      421 atgggttgtc gtggcccgag gcagctggag gaactccgat aaatgcggat acccacgtga
      481 tagaatgttc atgggttgca gatggagcag acaataccga ctggtgggaa gccgtcgtcc
      541 cacccatgtc ggatggaact gttttgcgct acaaggtcgg ttcattcaag acgcaaaatg
      601 atttcaatgg ctgggatgtt cccttcccca acagcaccgg cgacattgag cgcaaaacaa
      661 aaatgatggg cgaatgggaa attgccggct ttgatccgtc cacggtcgtg tatcgcccgc
      721 acaacgactt tggcctgacg gccaccggcc ttgtggacgg tttccatttc ctgcaggcgc
      781 gggcgttcct gcagcgcgac ggcaaagcgg cgatttacaa caccttcccg caaacgttct
      841 atctggacac ggaaaccccg cggggcgaaa tcaaatatcc gacggccgga gaaacactct
      901 ggcagaacga atacggcgcg gtggtccgca cagacccgac cgtcagggag gtctggtacc
      961 atattgaaga cggcaatgcc gccaacgatg acggtcagac cggcaacgat tatgggaacg
     1021 gaaccaatgc gcttggggag gctgcgtgga tgcaggccta ttccgttaca ccgagcctga
     1081 gcattgacag cgaatatccg ctggagtggc gcttcagcta tcacaacatc cccaccaatg
     1141 gcagcggggt catttatgtc cgcctgctgg aaatttcctc ctccaccaat ttcagcctga
     1201 accccgtgga cggacactgc actgaactgg ttcgcaccaa cagcactcag gcgccggcgg
     1261 ttacgttccg tttcaactgg ccggatgccg acggattcac tctgcaggag ggctggacca
     1321 ttaagctgga tttcagcacc tcactcggtg tgccgggcga tgaaagccgc ttcctgatca
     1381 ggattaacga tgtggctcaa agtcgtgacg attacaggat cgattggggc accggccggc
     1441 tggaattcga catgcctgat ctttacaacg gcgattccaa tttcctgcat cacatcgcca
     1501 tcacctatga gatgtatggc ggggttgtgc tccaggccca tcgttacatc aaattccagt
     1561 ccaccgcatc cgggccgctg gtgcagatcg tggatccgcc cgaggtggat tctgatgggc
     1621 agccgttcaa aattgtgctg cctgatgtgg cctcgcccag tcccacacag aggcagtata
     1681 ccatccaggt cgagacggac ctggccgcgc gcgacgtctg gattgccttc acgaattcag
     1741 ccggagaagc cgtgaagctg gcctctgtca ccaatgattt gacgggtacc gtttccgcgg
     1801 tgaccggcag cacgcttctg acgggcaccg gaacgctgtt caactcccag ctttcggttg
     1861 gaagcacgct tctgctgggc accaatttcc tgaatgtgac gcagattgtt tcggggacca
     1921 acctgtatct gagcagcccg tggaccgaag cctcggcgtc cggcatgacc gcgtatcgtg
     1981 tagacggcaa tccggcggtg tccgggaaca agcagatttg gcgtttcctc tggaccaaca
     2041 tgacggccgg atatttctcg ttccgcgcga atgtggacac aaacgggaac aacgccacgg
     2101 tggaggccta tgcgtcgcgc aacgtgaccg tgctgttccg cgaaatggcc ggcagcagca
     2161 cgaacgacta tgatgacgat gacgatggaa tctatgattc caacgaggcc gcctacacca
     2221 atcttccgtc atcgaatccg gagacgtggc tcaacgggga tgtgcatatc tggatgattt
     2281 acggccgcac cgatccgctg ctgcccgaca ccgacgggga cggcctcccg gacggactgg
     2341 aatccggctg gcgcgccccg attgatcccg gccagaccga taccaatgcg gacacaaacg
     2401 gcgacggcta tcccaacttc cttgccgatc ttgatccgcc tttcttcaac actgtccctg
     2461 acaactggga tgtgccgaac tacaatttca acggattgcg cacggaaaag atcgccgggt
     2521 ccatgacgga tcccgcgaat ccagattcgg attatgacgg cattcccgac gggattgaag
     2581 actggaaccg gaacgggtgg gtggatggag acggagatcc actgtatccc gggcaggaca
     2641 aatccacgcg cgggagctgg cctgacggac agcgggattc gttctgggcg gagaccgacc
     2701 ccaataattc cgacacggat ggagacgatc tcacggacgg ctacggcgag gacaaggatt
     2761 ccaacggctg gatagccggg gacattaatt ccaaccgcat ctgggagacc ggggaactct
     2821 ggctggagac cgatccgctg aatcctgata ccgacgggga cgggctgcct gacggctggg
     2881 aagtccgcta cggccttgat ccgtgggact gcggcattgt cgggcgcaca aacatgcaga
     2941 ccggcgcaat catcacgaac attctgaatg gggccgccgg taatccggac ggcgatacta
     3001 ttattgtggg gctgtccacc aacgaatata ccaacctgct ggagtatcag aacgggacaa
     3061 atccgcggca gccggacacg ggcgagcccc cgccggaggg ggcggtcgtg atcggccgcg
     3121 gcgaggaaat cggaagtctc accggaagcg gcgcccatta ccaggaattc actgattgga
     3181 ccgttgagga ctgtctggtg ctggacgaat acgagggcgg tggaaacaac aaccagggcg
     3241 gcgacgtatt caagggctgg gacggtttcg acgagtcgcg cgacatcatc gcgttttacg
     3301 cgcgtgacgg cggtcctctg gcgagcggcg gagatgacaa gttctatttc cgcgtggatt
     3361 tttatgatct gaaacccctg gcggaagagg gcaacctgga tctttatgtt gtcgttgaca
     3421 ccggcaatcc ggcctccggg gagatgaatc ttccggacga cgtggatacc attacaagca
     3481 accgctggga ggcgatcgtg gcggtgtatc agtcggggca gggccgtgtg tatgttgata
     3541 ctgcacaggg gaacaacagc acgacatggg gtcaggatct ttcgagtttt ggcgtcgaag
     3601 cccgcgacca gaattccgcc gatggtttta tagatgccta ttacaattcg gaactggatg
     3661 ctgttgaatt tgcgatttca cgccaggccc tgctggatgc cggatggaac gggctcaacc
     3721 cggatgacct gaactatcag gtcttcacca caaaggacgg cacctgcaac ggctgcggcg
     3781 agggcggaaa tccgggcgga ggcgacatcg gcggccgcag tgatgtgcgt gatgcaatat
     3841 ggaacgactt tatcgctgaa gattactggg aatcccaggc cggcctggac agcatcctta
     3901 aatactggat tcccggctcc acgcgcgccg ggcagaccaa ggtggctgtg gtggtgcacg
     3961 gcaatcaggc gatcagtccc ggaagcgaga tcatgaatct ggtcaacgac aacgccggcg
     4021 ccggctacaa ccggccgctg gctgtgcacg atctctacaa ggctcccctg aatctgcaca
     4081 ttaccccgac tctggcatcg gccattcaat gggcctcggt cgatacgaat gcagggaagc
     4141 cctggctgga cggccctgcg ctcaacagtt atatccacga tctggtggca accaatgtga
     4201 tctatctgct gggaagcacg ttttcggacc acatccttcc gtatttcacg aaggaattca
     4261 atgcggacaa tgtggcgctg gcgaatgatt atctgtcgga tatttacggg ttcaccccct
     4321 cgtcaaacac cgtgttctgg acgcccgagc gcgtgctgga cgccgatgtg ttcgacaaga
     4381 tcagggcaat gggctactca tacacgattc tggaccaggg gacgcatctg tggcactggc
     4441 tgggccgcac aacggctctc ggcgagtacg gctatcgaat caacaaaatc aacgatgtga
     4501 actgcttcgt gatcaatgat ctgcccaccg gctaccggtt cactaatttc gatggcgggc
     4561 tgccggcggc cctgcggagt ctttttgtcc gcaaggggct cagcggcact caggatcagg
     4621 tggtgaccat tctcagcaac tgggaggatt tcggcgatct ggatgatgcc aatgcgtatg
     4681 acatcaacct gcgctggatg gcgaatcacc cgtggatccg catcgtcgcg ctggaggaga
     4741 tcgctgccgg acaggtggac atcaacaacg acggctgggg cgatgcgtgg tgggcggagg
     4801 accggggcgc cgcggccacc agtaaagttg cacacaactg gctgcatcat gccacccgca
     4861 gcgattacga caactggtat ctgggcgatg ggctggcgga agggctgcag aacaagatat
     4921 ttgacatccg ccccggagcg gccatgcctg cggcctacgg aatgatgtat acggcgggaa
     4981 ttgtcaccga cacgtgggat caggtaacag cgatcgccga ccccaatctg gctcatctgg
     5041 cgcggagcgt tctgcacgcg tcggtgtttc agaccgcgtt tcacaacagc agcgaagacg
     5101 acctcagccg ctggagcacc ggggaatata tctatcccgc cacgcagtcc aattccctgg
     5161 caggctttgc aaaaatggcc caggcacaga cgcgcaccgc ggcgatcttt gcctatgccg
     5221 atgactgggc cgcctcggcc ggaagtctga ccaccacggt tgtcgcgcag gcggatgttg
     5281 atctcgacgg ggaaaatgaa tatgtgctgg cgaacaaacg actgctcgga atattcgaac
     5341 ggattggcgg acggatgatt gcggcgtggg tgcgcagttc ttcgggacat gtgtatcagg
     5401 tcgccggaaa ccaggccggg tattccgcct ccgaaacgga agaggagggc atctcgaatg
     5461 tggaaaccaa cggaacggtt gtggcgcacc gcacctcgtg tttcaaggac tggtgggcgg
     5521 ggacatcgga ctatgtcaat gatctctaca cgtttacggc cacgaacaac gggtgggtga
     5581 ttcgttcagc agacagcaat ataacaaaga tgatcactct ggcccccggc accaaccagt
     5641 ttgaggctca gtatacggtg gacggatcgc tcaacggcgg ccagctgtat gtgcgtcatg
     5701 gcctttcgcc caacctgtat aatctgctgc tgcgcggtca aagcaccctt ggatatgagg
     5761 agcacggcgg cggaataatg accctcgcga acaacggcga tggagtgaca atcagcgcgc
     5821 ggatcggtta cagcgataca ggacatactg cggcattcaa cagcagcgcc gtggatgaca
     5881 gtccgggcgg cggggttgaa ttcaacacgg ttaacatgcg caatcaggtg cagacgcatc
     5941 aggttgagat ttacggaacc ggcgcattca gtttttcact cggctttgcg gctgaggcgg
     6001 gactttcggc atgggagcag ttctgcgctg attacggata taatcccgag gattatacgg
     6061 cggatgccga cggtgacctt attcccaact ggggcgagtt tgtttccaat acaaaccccg
     6121 aaaactcgaa tgatttcttc cgtgtggaaa acgccgcgcc agttcccggc ctgcagctca
     6181 gttttgatac cgcaaccggc cgtgtctact acatctggta cagcaacagc ggcctgatta
     6241 atccggcctg gagcaacgcg actccggccg ggataacggg ccagggcggg agtacgaact
     6301 ggacagatga cggatcgcag acggccccgc ccccggggga agttcccgac cgcttttaca
     6361 aaatcgaagt ggaatggccg gaataatatt tcttcgtcaa ttggcgtttc cgggcggcat
     6421 attcatcata tgaaatctgt atcccggcca ctctatttgc acgaagatgt tttctgagta
     6481 tattatccaa tgagcaagtc atggtcagtg ttagctgcgg ggatgatcgc gtggcctgtg
     6541 ctgggcgcgc ccgtccgcag cggaccggtg gaagcggaac tggttcccga agtggaaaac
     6601 atcgcccccg gtcagccgtt cacggtcgct ctccgcctga agatggatcc gccctggcac
     6661 acctattgga tcaatccggg cgattcgggg cttgctcccc gcctggactg gtcactcccg
     6721 gaaggatttt ctgcggatga acttcttttc ccgctccctg cagccatccc gacaccgccg
     6781 tttatgacct acggatttgg gaacgaggtg tggctgctgt ccacgataac ccctccggcc
     6841 gtcctgaaat cggatcgcgt tgtcctgaag gccgtggtgt cctggctgat ctgcaatgaa
     6901 gtctgcgtgc cgggttcggc cagccttgtg ctgcgcctgc cggttcaggc cggcggcgca
     6961 aaacctcatc ccgttcatgc ggctcaaatt tccgccgcgc gtgcgcgcct ccccgaaccg
     7021 gcggccggat ggcaattccg tgtgcgtcat gaaaacgggt tgtatcatct ggctgtgttt
     7081 ccgcctcccg gatacgcggg agaggttcag tcagcatcct ttttcccgtt tcagaacgat
     7141 ataattcaac actcagcccc ccagaaatgg cggcgcagcg cagaggggta caccctggaa
     7201 cttgtgcccg ccgctccggc tgcttccccg gaatctttgt cgggcgttct tgtaatcgat
     7261 gagcgggatt cgcgccgcgc gcttcgcgct gaggccgttt ttccccggac agagacgccc
     7321 cgcgatttaa acaaagaaag gaacacacca tgaagaaaaa gataatattg acggctctgg
     7381 ctgtcctcgc actgtttgga acggcctttg caccggctca ggtctccgtg ggatcgccgg
     7441 ctcccggatt caccctgccg gatgcgcagg gtgtcagccg cagcctttcc gaattcagcg
     7501 ggaaggttgt gattctggag tggaccaatc ctgactgtcc atttgttaaa aagcattacg
     7561 actcgaagaa catgcaggcg ctgcaggaga aatacatagc ccggggcgca gtctggctgt
     7621 ccatctgctc ctctgctccc gggaaacagg ggcattacag tgccgctgaa tggaatgata
     7681 ttcttggccg gcagggcagc cgcgcgacgg ctcttctgct tgatcctgag ggcagggccg
     7741 ggctggccta tgccgcaaag accacgccgc acatgttcgt cattgatgcg gaaggaaccc
     7801 ttgtgtatga cggggccata gatgaccagc cttcgacacg gacggatgat atccccggcg
     7861 cgaaggcgct gcttgccgac gccgcagatg ccgcgctggc cggacagccg gttcctgttc
     7921 cgaaaacggc accctacggc tgttccgtca aatactgatg ccggcaggcg ggcctattcc
     7981 attatccggg gctgcccggt gacggaaatg acgctttccc acagttcgct ttcgggctga
     8041 acatatctcc ggcagcaggt ggcttccgcg agcggcacgt ggatgaacct gtcgttccag
     8101 aggccgatca gcacatcggt ctttccggcc atggcggcat gcaccgcatg gcgcgcgaac
     8161 tgatcgcagc ggatgctgtc agcacagttg gcgggcacac tccggatgat gtagctcgga
     8221 tcaatatact ttggatttgc ctcaatattc ttctccctga agtatttttt gatctccgcc
     8281 agcaggaacg ggccgatgtc gttgtgcagg atgttcccgg aggcatcccg gcggcattca
     8341 tccttcggca tgagttcctg tccggccccc tcagccacaa cgatcagggc atgcggtttt
     8401 cgggccagcc gcctttccag atgactgagc agcccatttt ctccgcccag tgcgaatttc
     8461 acttcgggaa tcagtgtgaa atccacatcc tggctcgcca gcgatgcacc ggctgcaatg
     8521 aatccggagt cgcgccccat gactttaaca agtccaatgc cgttgattgc ggcttttgcc
     8581 tcaatatgcg cgcagttgag aacctcatgc gcgcgttcaa tggatgtgag atagccaaag
     8641 gtctgctgga cataggggat gtcgttgtcg atggttttgg gaattccgac aatcgccttt
     8701 ttcaggccgc gccgggtcac ttcccccgca atggcatggg ctcccctctg cgtgccgtca
     8761 cctccgatgc agaaaagaac gtcaatgtcg cgggattcga ggaaatccgt catcacctca
     8821 acgggttgtg cgccgcggga agagcccagc actgtgccgc cctgaagatg aataccgtcc
     8881 acggcctcct cggtcaagac aagcggcggc cggccggccg cgggattcaa cccctgatag
     8941 ccgtagcgga tgccgagcac ttccttcgcg tgatagttct gcttcagctc aaagcaaatt
     9001 gaacggatca catcgttgat gccgggacag agtcctccgc aggatacgat ggctgcgcgg
     9061 accttcgggg catcaaagaa gattttttcc cttggcccgg ctttttcaaa gagaatatcg
     9121 ggattgtcgt aaggtgtgtt gaactccacc tgatagcgga cacgggcagt gtcgggaatg
     9181 aagtctccga tcccgtcgcc gggaacagtg gacaggctca gaggggagcg gaagcggcag
     9241 cccccgagcg tggcgatttc cattttgttt tctgatgcca tgaatgttct ccgcagttac
     9301 attaggccgc agtctagcgg accggggcct gatgttcgac aactaaatcc gtcaaatcgg
     9361 aacttggatt ccggcactgc ggctctatac tgaagcagac ggtgaatcac aaagaggcgg
     9421 aaccatgacg aataaagtat ttctgcagtg gtgccagata cggagaaaag tcgggggtct
     9481 gacgggtcat tcgctggaca gcaatgtccc ggacatctgg actcccaacg cggatgtttt
     9541 tgaaagcgcg gatggcgtga tcatccgcat ggaactggcg ggggtgccgc tggacaggct
     9601 ggagatcacc tgcgaaggcc gtgtgctgaa tatcagcggg gtgcgctgtg aatcctttgg
     9661 cgcagaaacc cttgccggat ttcgcttccg ccagctggaa atcgagtacg gaccgtttca
     9721 ccggtcggtg acactgccct atgaaaccga tccggcgcac acccgggcaa ccatgaagaa
     9781 cggcatgctg gaaatccggg tgcgcgcacc gcgtgcggca aagaaaacaa tttcgaaaac
     9841 ggggaaaaga taggttatgg ctgatcagaa aaagaatgtc agatccacgc ttccggcaaa
     9901 ctccgatatg cccggtcgtg acgcgccggt ggtgctgccc gtgctgcccc tgaatgatct
     9961 ggtgctgttt cccacgatga ttattccgct ggcggtgtcc acgcagcgct ccatccggct
    10021 gattgatgac gtggtggccg gaagccgcca tttcctggcc gtcctgcagc gtgaccgcaa
    10081 gctggacgat gacaaggtgg agccgcctga tctgcacgag tttggctgcc ttgcccgcgt
    10141 gctgaaaatg atcaaattcc cggacgagac ggtgcgtgtt ctggtgcagg gcattgtgcg
    10201 gtgccagatc cgcagctatc tgcccgagaa cggctatctg aaggcccgct acaccctgct
    10261 tcaggacgaa atggaggaca gcattgagct tgatgcgctg gcgcgcaacg cttcgcagcg
    10321 cttccaggac atcattacga tgtcgcccgc catgccggaa gaactgaagg tggccgcggt
    10381 gaacatggcg gatgccggga aactggcgga tctgattgcc tccaacgtga acatccccct
    10441 tgaagaccgc cagcggcttc tggaggaata tcgtccccga aggcgtcttg agaaactgac
    10501 cacgcagttg aatcgcgagc tggaaattct tcagcttgga acacagatac agagtaaagt
    10561 cagcgaaacc ctctccaaat cgcagcgcga ctattatctc cgggaacagc tcaaggccat
    10621 tcagagcgaa ctgggcgagg ataatcccca gcagaccgag atgaaggaga tcagcgggaa
    10681 aatcaaggcc gcgaagctcc cgcccgatgt ggcggaaatt gcggacaagg aaagaaagag
    10741 gctggagtcc attccgtccg cctccccgga atatgcggtg atccgaacct atctggactg
    10801 gctcacggag ctgccctggg cggtggagac ggtggaccgg ctggatattg ccgcggcgaa
    10861 gaaggtgctg gatgcggatc actatgatct ggcgcgcatc aaggaccgga tcctcgaata
    10921 tctttcggtg ctgaagctca agaaagacat gaaaggcccg attctatgct ttgcgggccc
    10981 gccgggcgtg ggaaaaacct cgctgggcaa gtctattgcc cgcgcgctgg gacgccggtt
    11041 tatccggatg tctctcggcg gcgtacatga cgaggcggaa atccgggggc accgccggac
    11101 ctatattgga gccctgccgg gcaggatcat ccaggggctg aagcgcgccg gcacgcgcaa
    11161 tccggtgttc atgcttgatg agatcgacaa ggtgggcgcc gatttccggg gagatccttc
    11221 gtccgccctg ttggaagtgc tggatcccga gcagaattat tcgttctccg atcattatct
    11281 caacgtgccc tttgatttgt cgcacgtgct tttcatcacc acggccaaca ccatggatac
    11341 catccctccg cccctgcggg accgaatgga ggtgctggag ctgagcggtt atacgatgaa
    11401 cgagaaactg catattgctc agaaattcct gatcagaaag gagattgccg cgcatggcct
    11461 gaagtccgga cagattgcct ttgaaaaggc ggccctggaa aagatcatca gcgattatac
    11521 ccgggaggct ggtgttcgca atctggaacg cgaggtggcc aatgtgtgcc gcaaggtggc
    11581 gcgcagcgtg gccgagggga agaaggaccg gacagtgatc accccccaga gaatttccca
    11641 gatgctggga ccggagaagt atgagagcga agtggcggag gcgcgaaccg aaccgggcat
    11701 tgttaccggc ctggcatgga ctccggttgg agggcgcatt cttttcatcg aggccacgca
    11761 gatgtccggg aagggacagc tgatcctgac gggatcactg ggcaacatta tgaaggagtc
    11821 cgcgcgcgcc gccctgagtt atgtccaggc gaattcaaag aagctgaaaa tcgaagtgcc
    11881 cgaagagtcg ggaaaagaca ttcatatcca tgtgccggct ggtgccatcc caaaagacgg
    11941 gccgtcggcc ggagtggcca tgaccatggc gctgatcacc ctgatgactg gccgccccct
    12001 tccggccgac atcgccatga ccggcgaaat cacgctgcgc gggaaggtga tgcccgtcgg
    12061 cggg
//
LOCUS       DAOQWR010000074        12024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5788_length_12024_cov_5.471719, whole genome shotgun sequence.
ACCESSION   DAOQWR010000074 DAOQWR010000000
VERSION     DAOQWR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_12024_cov_5.471719"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..137)
                     /locus_tag="PKK36_07125"
     tRNA            complement(50..137)
                     /locus_tag="PKK36_07125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Ser,seq:tga)
     gene            281..1237
                     /locus_tag="PKK36_07130"
     CDS             281..1237
                     /locus_tag="PKK36_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNR94366.1"
                     /translation="MKLLKRQPMMRRVILATLPCVAGSVYYFGWRSLAVIAVSCLAVF
                     LTEYAFCRRRKEPVTEAVFVTGILYALVMPPTVPWHVLIIGVVFAIAFVKEAFGGFGR
                     NVFNPAVAGRCFVYVCFPVAMTSTWAPVSQGPLGALNQWTTAGVDAITSATPMALMKA
                     GEISLSSSDIFHGLFLGRIAGTMGVTSALLILVGGGYLYFTKTANRTLIITVIATYAV
                     LNQVLHWLGVEPVPGALPAVLGGGFLFGAFFMVTDPISAPSTQGGRIAYAMLIAVCTL
                     IIRNFSIFNGGLMFSILIGNMFGPILDHAAKALKNTGRGAAE"
     gene            1234..1914
                     /locus_tag="PKK36_07135"
     CDS             1234..1914
                     /locus_tag="PKK36_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNR94367.1"
                     /translation="MTEKPAIKFRTRRSFPVLYMFAVTFVATGILIGLSRSTAGRVEA
                     NKQILFERAVLSALGVDAGQRPSPANIHQTFLAQVKSPTESTAGAYRLVRDGATAAYA
                     LPFAGRGFWNEIRGVMGISPDGSVLTGVAFYEQSETPGLGAEIVKPAFTEQFRNLKLA
                     GGSSALGLKPAGTKTSASEVDAITGATQTCTRLEDILNDALGAWRREMGLHESGGAAA
                     PDGSGEDG"
     gene            1914..2555
                     /locus_tag="PKK36_07140"
     CDS             1914..2555
                     /locus_tag="PKK36_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNR94368.1"
                     /translation="MNGAGGGQKSVKALAMEGLWTNNPIFGQVLGICSTLAVTNMVMN
                     TLVMCAALVVTLSMSSLSISLLRSWTPRNIRMMIETLIIAFYVIMVDIALKAYWPEMS
                     QNLGPYVGLIITNCIVMGRAEACANTQPPLTALVDGFFNGLGYSFMLLLVAIPRELLG
                     MGTIMGFAAPYFSGPHWDKWIIMVMPPGAFFMLALAVWLFRSIQGPQQKEGAK"
     gene            2552..>2837
                     /locus_tag="PKK36_07145"
     CDS             2552..>2837
                     /locus_tag="PKK36_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNR94369.1"
                     /translation="MNTDGVLLQALVVAFSAAFTGNILLTNFLGMCPFLAVSRQVKTS
                     IGLGWAVLFVMTGTCMLNWVVYWHVLVPLHLEFLRFIMFIIMIAAFVQFVE"
     assembly_gap    2838..2937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2938..3309
                     /locus_tag="PKK36_07150"
     CDS             <2938..3309
                     /locus_tag="PKK36_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNR94370.1"
                     /translation="LRFIMFIIMIAAFVQFVEMLLERYSPVLYQNLGIFLPLITVNCA
                     ILGAALFMVIREYTFVNTVAFGLGSGAGWMLAILAMAGIREKMKPDRIPQGLQGPGIT
                     LIIVGLMALAFMGFTGVLSGG"
     gene            3323..4417
                     /locus_tag="PKK36_07155"
     CDS             3323..4417
                     /locus_tag="PKK36_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNR94371.1"
                     /translation="MADMFLSILIVSAIAAALAALLVVAQRYLANYGKCRIKINDSKT
                     IEVEGGSPLLESLAQNKIFIPSACGGRGTCAYCKVKVMEGGGPVVPTEEPYLDEGERK
                     DGVRLSCQVKVRNDLRIEIPEELFSVREYLCTCTRIRELTHDIREFRFELKEPAEITF
                     VPGQYVQLFSPAYDGNEEVYRAYSIASDPAEKNIVDLVIRLVPGGICTTWCFEHLKEG
                     AEARFNGPYGQFRLSDSDAPMIFVAGGSGMAPMKCMLHHMKNTGNKRKTTYFFGANLV
                     NELFYRELMQEFEQSLGSFRFVPVVSRPGEGDPWDGETGLVTEALRRNIKDAAGCEGY
                     LCGSPGMIDAAVKVLVELGVPENKIFYDKF"
     gene            4427..4879
                     /locus_tag="PKK36_07160"
     CDS             4427..4879
                     /locus_tag="PKK36_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94372.1"
                     /translation="MKRSPDMQRLEETLRSSKIAGGGFLGSDPRPLEEILDADAAELA
                     RLGHTQYELADRMRHISSRAEQGLGSWVAVDEIIEAAVTDTRGLMPCPWPHPGTFSKT
                     VVTARNLRTGKTIQWSELNIHLIEMHGFFEGRGSAFRLEPAELADILF"
     gene            4999..5454
                     /locus_tag="PKK36_07165"
     CDS             4999..5454
                     /locus_tag="PKK36_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNR94373.1"
                     /translation="MNKEQRNCISQCAGMALLRWALGLLFLAGGVAKILNLEGFVSGY
                     LVPAFEKTFLPGWMIAAYGYALPFVEALLGISLILGVCRTPALLLTGLTLISLAFGQM
                     LLQNHATVANIMLYILMTAVALLFPQYDGWVVPCCGLKCGLSPSEQKPD"
     gene            complement(5451..6560)
                     /locus_tag="PKK36_07170"
     CDS             complement(5451..6560)
                     /locus_tag="PKK36_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR94374.1"
                     /translation="MKRVVIIGGGFGGLACARALSGCRTAEVTLLDKKETSDFLPVLP
                     DILGGRVSPDTARAPLAARAKREGFRFVQDEVARVDISGRLVHGRDNLYEYDRLIIAA
                     GTQTNFYGDETTARLAFKLDSVRDAEQIGAAIRETSFDTVVVAGGGYTGIEVATSLRR
                     LAQTHQLNFRIIIAEASRRILNALPEWMAHYAENNLAHMNVEIALNCRASPVADNQVE
                     LSNGQIFQSAMLIWAAGVRTPSFVREIGPTAEKQERLAVDGCLQVADGIYAVGDSAAV
                     VAGGQSLRMAVQFAVAEGHLTGENIARSLNGKPMRAYRPRDMGYLVPMANHRAYGLVL
                     GQKLRGLPASLLHHAMCAAKSVGLRRKFRMLKEMF"
     gene            6742..7038
                     /locus_tag="PKK36_07175"
     CDS             6742..7038
                     /locus_tag="PKK36_07175"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNR94375.1"
                     /translation="MMKDGLAKSGNVIAREVAGENLLIPVCGKLADLQRIFALNPVAK
                     FVWDHLDGRRGPEALAAAVAEEFDVAPDAALTDVRELLGAMLKEGLISKAAGDD"
     gene            7031..8140
                     /locus_tag="PKK36_07180"
     CDS             7031..8140
                     /locus_tag="PKK36_07180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR94376.1"
                     /translation="MTEFCPESSVSEDFLRRLNRQAALARVPVAGSLELTSRCNFQCV
                     HCYLQHDEKDMPLSRIRSLLDQIAGAGCLFLLLTGGEPLLRADFPEIYEYAARKGLLL
                     TVFTNGSLIEDATVELFRRIPPQAVDITLYGASAETYRVVTGRGEGFEEVRSGIEKLR
                     AAGVRVKLKAVMLRQNEQDLLSIEKLARDFGASFRMDPVIFSRLNGDRDTLTFRVDPE
                     KAVQLEFSSPRRAADWKKHFERASGMTPSGRLFSCGAGVTSFHIDSTGKLQPCIMTPY
                     IQYDLNSGSFAAGWRRISEAVTARDIPENWVCGSCRFRPLCGICPGYAWFETGAEDKP
                     ADYLCKLGQTRYMYLLEGAEDANRKKESEKTVSEA"
     gene            complement(8129..8308)
                     /locus_tag="PKK36_07185"
     CDS             complement(8129..8308)
                     /locus_tag="PKK36_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94377.1"
                     /translation="MNREVEAADMQIRHAVQDPVIWQLLDVQVNPVGAEELSFLHPMP
                     RTSSATSSMVFSSGF"
     gene            8396..9118
                     /locus_tag="PKK36_07190"
     CDS             8396..9118
                     /locus_tag="PKK36_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94378.1"
                     /translation="MPINVRLLPLRDATPPASRTLFESPVWSMTADGDMRRIEMRGRG
                     CAEPYWVAQFQQPIRRVDVYFGPGQLTTDGELMSPLSYPLDQLLLMYLLAEAGGMLMH
                     AAGGVKTGGAVYAGPSGAGKTTLTRRLLAEGCEMFSDDRIIVRPWAGGLWLFGTPWPG
                     DANVAANRCAPLAHIYILRKSNADREEPLAPAEALAKIIPCCSLPWFDPPVLEQALQG
                     LNRILQDVPHSILHFTKEAAQN"
     gene            complement(9080..9502)
                     /locus_tag="PKK36_07195"
     CDS             complement(9080..9502)
                     /locus_tag="PKK36_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNR94379.1"
                     /translation="MKRILADSGTVFVPAAELVEEFLRSGTAVRFRVTGTSMHPSILA
                     GDVVTAAPCPADRIRRGQVICFRHEEEMRVHRVISAAPNALRAKGDAVNEFAEDIRPQ
                     NVLGVITSVEREGRRVSFSPPFRRFTSVLRGFFRKMKN"
     gene            9595..10035
                     /locus_tag="PKK36_07200"
     CDS             9595..10035
                     /locus_tag="PKK36_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNR94380.1"
                     /translation="MRRLIPVVLLLAGAVILCGEEAESAVAESSAISIRSAPIEGKIF
                     LLTQERGEEEVAGANVYVRIRRPRERGFLYETKTRKDGSYEIPGLEPQNYHMLVGDLR
                     LDLRVFPETEERTDFSKKLIIMIPLEMAGPGAGKPAEATAQKRP"
     gene            complement(10020..10637)
                     /locus_tag="PKK36_07205"
     CDS             complement(10020..10637)
                     /locus_tag="PKK36_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94381.1"
                     /translation="MSCRNLWGALAVLLAANTGAPAFEKMELIADIRPGSMILHTADG
                     DFEVKGPGPERLGTTEIEEAGTISTLPNVNIGVGFANPNLYLDLTATAGYLINSRFNS
                     LMLGANAALTFRPEKNFAVGPHLGLMQFTNPEWRGDAEMDISDSTALLLGVQASLGYD
                     VLFVLSVDYLYADPMDVKTKDAWIASDDELDISGMAIQFGIQGRF"
     gene            10979..>12024
                     /locus_tag="PKK36_07210"
     CDS             10979..>12024
                     /locus_tag="PKK36_07210"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNR94382.1"
                     /translation="MTLLITASVFPFLTSASSSLAEASAESAALQFTSPTPASQLSTL
                     GTFYSAQHPDWPPFPANVHDLPAWDLGNGCFLLADADFDYEAFAKAVEAVSYTSGEYP
                     MISRDYARDDLWISLNAVVNGRTDFTADLTIHTPETSTSASWDLYYARDLDWQTDWNF
                     LMRCPTNIIGQTNVYARYLPDEIAFFMARETTTNCDLIVTTNVTPQQMAELLVPPWVT
                     VTNVNYTGAAVARGIFANGNCCGLPIAGGVILSSGDITDATGPNDVDNKGEAVGGLPD
                     ADLNLLVGGSGSKDAAILEFDIISTNACLLQFRYVYTSEEYPEFCGSGFCDPFAVFVT
                     TNRVGANWINTISN"
BASE COUNT         2328 a         3332 c         3598 g         2666 t
ORIGIN      
        1 cttcccgcgc ccgtggtcgg tcgtagctca aaatccgaaa atcaggaaat ggcggagggg
       61 gagggattcg aacccccgga gccttgcggc tcgccggttt tcaagaccgg tgctttaaac
      121 cactcagcca cccctccatt gtcgaggctc tggcaatata ggagaccgga ttctgattgt
      181 aaagcgcgca ggggggaacc ggtccggtcc gggtgttgcc attggcacgg gaatgggtta
      241 ttctgcctcc gatcaagctc atagcgtgag aatttttttt atgaagcttc tgaaaagaca
      301 gcccatgatg cgccgggtga ttctcgccac gcttccctgt gtggccgggt ccgtctatta
      361 tttcggctgg cgctccctcg ccgtgattgc cgtttcctgc ctggcggttt ttcttactga
      421 atacgcgttc tgccgccgcc gcaaggagcc cgtgaccgag gcggtcttcg tcacgggaat
      481 tctctatgcg ctggtcatgc cgcccacggt tccgtggcat gtcctgatca tcggcgttgt
      541 gtttgcgatc gctttcgtca aggaggcctt cggcggcttc ggccgcaatg tgttcaatcc
      601 ggccgtcgcc ggccgctgct ttgtttatgt ctgttttccc gtcgccatga ccagcacatg
      661 ggctccggtc tcgcaggggc cgctcggggc gctgaaccag tggaccacag ccggggttga
      721 tgcgattacc tccgccacgc ccatggcgct gatgaaggcc ggcgagatca gcctctcctc
      781 atcggatatt tttcacggac tgtttctggg cagaattgcc ggcacgatgg gggtgaccag
      841 cgcgctgctt atcctggttg gcgggggcta tctgtatttc accaaaaccg cgaacaggac
      901 gctgatcatt acggtcattg cgacttatgc cgttctcaat caggtcctgc actggctggg
      961 cgttgagccg gtgccggggg ctctgccggc cgtgctcggc ggcggcttcc tgtttggagc
     1021 tttctttatg gtgaccgatc cgatttcggc tccgtccacg cagggcggtc ggattgcata
     1081 tgccatgctg atcgcggtgt gcacgctgat tatccggaat ttctcaatct tcaacggcgg
     1141 gctgatgttt tccatcctga tcgggaacat gttcggaccg attcttgacc atgcggcaaa
     1201 agcgttgaaa aataccggca ggggtgcggc ggaatgacgg aaaagcctgc cattaaattc
     1261 agaacccgcc gctctttccc ggtgctgtat atgtttgcgg tgacatttgt cgcgaccgga
     1321 attctgattg gtctcagccg ttcgacggcc ggccgggtcg aggccaacaa gcagattctg
     1381 ttcgagcggg ccgtcttgag cgcgctgggc gtggatgcgg ggcagcggcc ttcgcccgcg
     1441 aatatccatc agacttttct tgcgcaggtg aagtccccga cggaatcaac cgccggggcg
     1501 taccggctgg tgcgcgacgg cgccacggcg gcctatgcgc ttccgtttgc aggccgcggc
     1561 ttctggaatg aaatcagggg cgtgatgggg atcagtcccg acggctcggt gctcacggga
     1621 gtggccttct acgagcagag cgagacgccg gggctgggcg cggaaatcgt caaaccggct
     1681 ttcacggaac agttccggaa tctgaagctg gcaggcggga gttcggctct cggcctgaag
     1741 cctgccggaa caaaaacctc agccagcgag gtggatgcga tcacgggcgc cacgcaaacc
     1801 tgcacaaggc tggaggatat tcttaatgac gcgctggggg cgtggcgcag agaaatggga
     1861 ctgcatgaat ccggcggcgc cgcggcaccg gacggaagcg gggaggacgg ctgatgaacg
     1921 gagcgggcgg cggacagaaa tccgttaagg cgctggcgat ggaggggctc tggaccaaca
     1981 acccgatctt cggccaggtg ctgggaatct gcagcacgct ggcggtcacg aacatggtga
     2041 tgaacactct cgtgatgtgt gcggcgctgg tggtgacgct ttcaatgagc agcctgagca
     2101 tttcgcttct gcgcagctgg acaccgcgaa acatccgcat gatgatcgag accctcatca
     2161 tcgcgtttta cgtcatcatg gtggacatcg cgctcaaggc ctactggccg gaaatgagcc
     2221 agaacctcgg cccgtatgtc ggcctgatca tcacgaactg cattgtgatg gggcgcgcgg
     2281 aggcctgcgc aaacactcag ccgccgctca cggcgctggt ggacggattt ttcaacggcc
     2341 tcggctacag cttcatgctt ctgctggtcg ccatcccgcg tgaactgctc gggatgggaa
     2401 ccatcatggg ttttgcagcg ccgtattttt ccgggcccca ctgggataaa tggatcatca
     2461 tggtgatgcc tccgggggcg tttttcatgc tggcgctggc cgtctggctt ttcaggtcta
     2521 ttcaggggcc ccagcagaaa gagggtgcga aatgaacacg gatggcgtgc ttttgcaggc
     2581 tcttgtcgtg gcgttcagcg ccgcgttcac ggggaatatt ctgctgacca atttcctcgg
     2641 gatgtgtccg ttcctggcgg tgtcccggca ggtcaagact tcaataggcc tgggctgggc
     2701 cgttcttttt gtcatgaccg gcacctgcat gctcaactgg gtggtttact ggcatgtgct
     2761 cgtgccgctg catcttgaat ttcttcgctt catcatgttt atcatcatga ttgccgcgtt
     2821 tgtgcagttt gtcgaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt
     2941 cgcttcatca tgtttatcat catgattgcc gcgtttgtgc agtttgtcga aatgctcctc
     3001 gaacgctact cgccggtttt gtatcagaat ctcggaattt ttctcccgct gatcaccgtg
     3061 aactgcgcca ttctgggcgc ggcgctcttc atggtcatcc gcgaatacac ctttgtcaac
     3121 acggtcgcat tcgggctggg aagcggcgcc gggtggatgc tggccattct ggccatggcg
     3181 ggcatcaggg agaaaatgaa gccggacagg attccgcagg ggctgcaggg cccgggaatc
     3241 acgctgatca tcgtgggact gatggcgctg gcgttcatgg ggttcacggg ggttctgagc
     3301 ggaggttgac gggtatttgc ctatggcgga catgttttta tccattctca tagtaagcgc
     3361 catcgcggca gcgctggcgg ccctgctggt tgttgcccag cgatatctgg ccaattacgg
     3421 caaatgccgg atcaagatca atgattccaa aaccatagag gttgagggcg gctcgccgct
     3481 gcttgaaagc ctcgcccaaa acaagatatt cattcccagc gcgtgcgggg gtcggggcac
     3541 ctgcgcctac tgcaaggtga aggtgatgga gggcggcggt ccggtggttc caaccgaaga
     3601 accgtatctt gacgaagggg aaagaaaaga cggggtccgg ctgtcctgtc aggtcaaggt
     3661 caggaatgat ctgcggattg aaataccgga ggaattgttt tcggtcaggg agtacctctg
     3721 cacctgcaca agaattcgcg agctgactca tgacatccgc gagttccggt ttgaactgaa
     3781 ggaaccggcg gagatcacct ttgttccggg ccagtacgtc cagttgttca gtcccgccta
     3841 tgacggaaac gaagaggtgt accgcgccta ttccattgcc tccgatccgg cggagaaaaa
     3901 cattgtggat cttgtgatcc ggctggtgcc gggcggcatc tgcaccacat ggtgtttcga
     3961 acatctgaag gagggcgcag aggcgcggtt caacggcccc tatggacaat tccgtctcag
     4021 cgacagtgac gccccgatga tctttgtggc cggcggttcg ggaatggcgc cgatgaaatg
     4081 catgcttcat cacatgaaga acaccggaaa caaaagaaag acgacctact tcttcggcgc
     4141 gaatctcgtg aatgagctct tctaccgcga gttgatgcag gaattcgagc agtcgcttgg
     4201 aagttttcgc tttgttccgg tcgtctccag acccggggaa ggcgacccgt gggacggcga
     4261 aaccgggctg gtgacggaag ctcttcggcg caacatcaag gatgccgccg gctgcgaagg
     4321 ctatctgtgc gggagtccgg gcatgattga tgccgccgtc aaggtgctgg tggaacttgg
     4381 cgtgcccgag aacaaaatat tttatgacaa attctgagga gattgaatga agcggtcacc
     4441 tgacatgcag aggctcgaag aaacgctcag atcctcgaag atcgccggag gcggatttct
     4501 gggaagcgat ccccggccgc tggaagaaat tcttgatgcc gatgccgcgg agctggcgcg
     4561 gctgggacat acacagtatg aactggctga ccgcatgcgc cacatctctt ctcgcgcgga
     4621 gcaggggctg gggtcatggg tggcggtgga cgagatcatc gaggccgcgg tcacggacac
     4681 ccggggcctg atgccctgtc catggcctca tccgggaacg ttctcgaaaa ccgttgtgac
     4741 cgcgcgaaat ctccgcaccg ggaaaaccat tcagtggtcc gagctcaaca tccatttaat
     4801 cgaaatgcac ggattctttg agggccgcgg ctcggcgttc cgtctggagc cggcggaact
     4861 tgcggatata cttttctgat acggaactcc gttgttcgcc ttgcggcgga tttgcagtca
     4921 accacttcgt gcggtatact gttgcgctat acagataagg caatgccgcc ggatggaccg
     4981 gcataaggga gaacgtcaat gaataaagag caaaggaatt gcatatcgca gtgtgcggga
     5041 atggccctgc tgagatgggc gctggggctc ttgttccttg cgggaggcgt ggcgaagatt
     5101 cttaatctgg agggttttgt ttccggatat ctggtccccg cgtttgagaa aaccttcctc
     5161 cccggatgga tgattgccgc atacggctat gcgctgccgt ttgtggaggc gctgctgggg
     5221 atctcgctga ttctgggcgt gtgccggacg ccggcgttgc ttctgaccgg gctcaccctg
     5281 atctcgctgg cctttggcca gatgctgctt caaaatcacg cgacggtcgc caatatcatg
     5341 ctgtacatcc tgatgaccgc cgtcgccctt ttgttcccgc agtatgacgg ctgggtggtt
     5401 ccctgctgcg ggctgaaatg cggcctgagc ccgtccgaac agaagccgga ttaaaacatt
     5461 tccttgagca tgcggaactt ccggcgtaat ccgacggact tcgcggcgca catggcgtgg
     5521 tgcagaagac tggcgggcag tccccgcagt ttttgcccca gcacaagtcc gtatgctctg
     5581 tgattggcca tgggaacaag atatcccatg tcccttggcc gataggcccg catgggtttc
     5641 ccgttcaggc tgcgtgctat gttctctcct gtcaaatgac cctcggcgac tgcgaactga
     5701 acagccatgc gcagcgattg tccgccggcg actacggccg cgctgtcccc gacggcatat
     5761 atcccatccg caacctgcag gcagccgtcg accgcaagcc gttcctgttt ttctgctgtc
     5821 ggaccgatct cccggacaaa tgaaggcgtt cggactcctg ccgcccaaat cagcatggcc
     5881 gactggaaga tttgtccgtt tgaaagctca acctgattgt ccgcgacagg actcgcgcgg
     5941 cagttgagcg caatctccac attcatgtga gccaaattgt tttcggcata atgggccatc
     6001 cactccggaa gcgcgttcag tattcggcgg gaggcttcgg ctatgatgat cctgaaattc
     6061 agctgatgtg tttgcgccag gcgccggaga ctggtggcca cttcaattcc ggtgtagccc
     6121 ccgccggcca caacaacggt gtcgaatgat gtttcccgga tggcggcgcc tatctgttcg
     6181 gcatcgcgga cgctgtccag tttaaatgcc aaccgggccg tcgtttcgtc gccgtaaaaa
     6241 tttgtttgtg tgcctgcggc aatgatgagg cggtcgtatt catacaaatt gtcacgcccg
     6301 tgaacaagcc ggcccgatat atccacgcgc gccacttcgt cttgaacaaa gcgaaaaccc
     6361 tcgcgctttg cgcgcgccgc aagaggagcc cgggcggtgt ccggagaaac ccggccgccg
     6421 aggatatccg gcagcacagg gaggaaatca gaagtttcct ttttatcaag aagcgtgact
     6481 tctgcggttc ggcagccgct caatgcgcgg gcgcacgcga gaccgccaaa ccctccgcct
     6541 atgatcacca cccgtttcat gaagtcgcgc ctgctcccgt ccatccccgc ctttgtgtcc
     6601 gtcggtgagc atctgatatg atcgggccgc ggtcaacata ttcctgttca tggcaccgga
     6661 tcggcatcga ggaaaacaag caacagtctc gctttttgcg ccggttgcgg tatatttgcc
     6721 attattcagt gccgggaagg gatgatgaag gacggacttg ccaaatcagg taatgtgatc
     6781 gccagggaag tggccggcga gaatctgctg attcccgttt gcgggaaact cgccgatctg
     6841 cagcggatct ttgccctgaa cccggtcgcg aagttcgtct gggatcatct ggatggccgc
     6901 cggggacccg aagcgcttgc ggcggcggtt gcagaagaat ttgatgtggc tcccgatgcg
     6961 gcgctgacgg atgtgcgtga actcctgggc gcgatgctga aagaggggct gatctccaaa
     7021 gcggccggcg atgactgagt tctgtccaga atcatccgtg tcggaggatt ttctccgcag
     7081 gctgaaccgg caggcggctc ttgcccgggt gccggtggcc ggcagcctcg agctgacttc
     7141 cagatgcaac ttccagtgtg ttcactgcta cctccagcac gatgaaaaag atatgccgct
     7201 gtcccggatc cggtccttgc tggatcagat tgccggcgcg ggctgtctct ttctgctgct
     7261 gacgggcggc gagccgctgc tccgcgcgga ttttcccgaa atttatgaat atgcggcgcg
     7321 caagggcctc ctgctgaccg ttttcacaaa cggcagcctg attgaggatg cgacggttga
     7381 acttttcaga agaattccgc cccaggccgt ggacatcact ctttacgggg cctcggccga
     7441 aacgtatcgg gtggtgactg ggcggggcga aggctttgaa gaggtgcggt caggcatcga
     7501 aaagcttcgc gccgccggcg tccgtgtcaa gctgaaggcc gtgatgctcc ggcagaacga
     7561 gcaggacctc ctctccattg aaaaactggc gcgggatttt ggagcgtctt ttcggatgga
     7621 tcccgtgatc ttttcgcgcc tgaacggcga tcgggacacc ctgacgtttc gcgtggatcc
     7681 cgagaaggcc gtgcagctgg aattttccag tccgcgccgc gcggccgact ggaagaaaca
     7741 ctttgagcgg gcctccggaa tgaccccctc cggccggctg ttctcctgcg gggcgggtgt
     7801 cacgtcgttt catattgact caaccggaaa gcttcagccc tgcatcatga cgccgtatat
     7861 tcagtacgac cttaactcgg gctctttcgc tgccgggtgg cgccggattt cggaggccgt
     7921 cacggcgaga gatatccccg agaactgggt ctgcggcagc tgccgcttcc ggcccctgtg
     7981 cgggatctgt cccggctatg cctggttcga gacgggcgcg gaagacaaac cagcagatta
     8041 tctttgcaaa ctgggccaaa cgcggtatat gtatctttta gagggagctg aggatgcaaa
     8101 ccggaaaaaa gagtccgaaa aaaccgtatc agaagcctga gctgaagacc attgagctgg
     8161 tggccgagga ggttctgggc atcgggtgca agaacgacag ttcttcggcg cccacgggat
     8221 tcacctgcac atccaacaac tgccagatca ccggatcctg aacagcatgc ctgatctgca
     8281 tatcggcggc ttccacttcg cgattcattc cgtcgttccc ttgttttcct ctccgccgga
     8341 ccggccttcg gcagcttttt cctcacctcc cgccggtgcc gccgctgatg atgagatgcc
     8401 gattaatgtg cgcctgctcc cgctgcggga cgcgaccccg cccgcttcac gaactctgtt
     8461 tgagagcccc gtgtggtcca tgacggccga tggagacatg cggcgcattg aaatgcgcgg
     8521 gcgcggatgt gcagagccct actgggtggc ccagttccag cagccgatcc gcagggtgga
     8581 tgtgtatttc ggcccgggtc agctgacgac ggacggggaa ctgatgagtc cgctgtccta
     8641 tccgctggac cagctgctgc tcatgtatct cctcgcggag gccggcggga tgctgatgca
     8701 cgcggccggc ggcgtgaaaa ccggcggcgc ggtttatgcg ggaccatccg gcgcggggaa
     8761 aaccaccctc acgcgcaggc tcctcgcaga aggctgcgag atgttcagtg atgaccgcat
     8821 catcgtgcgg ccctgggccg gcggcctgtg gctgttcggc acaccctggc cgggagacgc
     8881 caatgtggcg gccaaccgct gcgctccgct cgcgcacatt tatattctgc ggaagtcaaa
     8941 tgcggatagg gaagaaccgc ttgcgcctgc ggaggcgctg gcaaaaataa ttccgtgctg
     9001 ttcgctgccg tggtttgatc cgcccgtgct ggaacaggcc cttcaggggc tgaaccgcat
     9061 tctgcaggat gttccgcact caattcttca ttttacgaaa gaagccgcgc agaactgaag
     9121 tgaatcggcg gaaaggcgga ctgaaactca ctcgccgccc ctcacgctcc acagaggtga
     9181 tgactcccag cacgttctgc ggccggatgt cctcggcgaa ttcatttacg gcatcgccct
     9241 ttgcccggag cgcgttcggt gcagcggaaa tgacccggtg cacgcgcatt tcttcttcat
     9301 gccggaagca aatcacctgc cctcggcgaa tgcggtccgc cggacaggga gcggctgtga
     9361 ccacatcgcc ggccaatatg gaggggtgca tgctggttcc ggttaccctg aagcgcacag
     9421 cggttccgga gcgaagaaac tcctccacaa gttccgccgc agggacaaaa accgtcccgc
     9481 tgtctgccag aattctcttc atagtgctga aaagctgttt gacaaatcct cgctttgaaa
     9541 cgaaattggc gcaagttaaa tcggttgaac atgaggatac aagcggggaa tttattgaga
     9601 aggctcattc ctgttgtcct gctgctggcg ggcgcagtca ttctctgcgg tgaagaagcg
     9661 gaatctgccg tcgcggaatc ctccgccatc agcattcgca gcgccccgat cgaggggaaa
     9721 atatttcttc tgacacagga gcgcggcgag gaggaggtgg ccggcgcgaa tgtctatgtc
     9781 cggatccgcc gaccgcgcga acgcggattt ctgtatgaaa ccaaaacgcg caaggacggt
     9841 tcctacgaga ttcccggtct tgaaccgcag aattaccaca tgctcgtggg ggaccttcgg
     9901 ctggacctga gggtcttccc ggaaacagag gagcgaacgg acttctcaaa gaagctgatt
     9961 atcatgattc ctctggaaat ggcgggcccg ggcgccggaa aaccggcgga agcgacggct
    10021 cagaagcgtc cctgaatccc gaactggatc gccatgccgg aaatatccag ctcgtcatcg
    10081 ctggcaatcc aggcgtcctt ggtcttcaca tccatcggat ccgcatacag gtaatccacc
    10141 gaaagtacaa acagcacgtc atagccgagg gaggcctgca cgcccagcag cagggccgtc
    10201 gaatcggata tgtccatctc tgcatcgccg cgccattcgg ggttcgtaaa ctgcatcagc
    10261 cccaggtgcg ggcccaccgc gaaatttttc tccgggcgga atgtgagcgc cgcgttggcg
    10321 ccgagcatca gcgaattgaa ccgcgagttg atcagatatc ccgcggttgc ggtcaggtcg
    10381 agataaaggt tgggattggc gaaacccacg ccgatattga cgttgggcag ggtggaaatg
    10441 gttccggcct cctcgatctc ggtggtgccc agccgttccg gcccgggccc cttgacttca
    10501 aaatcgccgt cggccgtgtg cagaatcatg ctccccggcc ggatgtcggc aatcagttcc
    10561 attttctcga atgccggcgc gccggtgttc gccgccagca gtacagccag cgcaccccat
    10621 aaatttctgc agctcatttt ttttctcctt gattcgggcc tgatctcgaa cctgcgcgaa
    10681 agtctacagc cggtttgccg gaaaaacaaa tcagaaaatt cgtttgtcgc cggcggaggg
    10741 gctcaaaaag ttccaatgcc tggaactttt tccggaaaaa cttccaatgg ttggaaattt
    10801 tcgcttttgc cgcgacgcat tcgctgccac gcccgattcc ggttgcccat ttctgcttga
    10861 ctgatctgtt tttttgtaac agggtcagcg catggacatg ttcttatcag acggttatga
    10921 tgagtgcatg cttccgcacc ggaggcgtcc gtttagtatc tggcgaatgt gtacccatgt
    10981 gactcttctg attacggcaa gcgtttttcc ctttctgact agtgcgtcgt cttcactggc
    11041 tgaggcttcg gctgaatctg cggctcttca gtttacttct cccacacccg catctcagct
    11101 ctcgacgctc ggaacattct attcagcaca gcatccagac tggccgccct tcccggcaaa
    11161 tgtgcatgat ctgcctgcgt gggacctggg taatggctgc tttttgcttg ccgatgcgga
    11221 ctttgattac gaggcctttg caaaagcggt ggaagcagtg agctacacgt ccggcgagta
    11281 tcctatgatc tcccgggatt acgcccgcga cgatctctgg atttcactca acgcggttgt
    11341 caatggccgc accgatttca ctgcggattt gacgatccat acccccgaga ctagcactag
    11401 cgcaagctgg gatttgtatt atgcgcgcga tctcgattgg cagacggatt ggaattttct
    11461 aatgcgatgt cccacgaata ttattgggca gaccaatgta tatgcgcgat accttcccga
    11521 tgaaattgcg ttcttcatgg cgagagaaac aacgaccaac tgcgatttaa tcgtcacgac
    11581 caatgtcacg ccgcagcaga tggctgaact gctggttccg ccctgggtga cggtaacaaa
    11641 cgttaattac accggtgccg ccgtcgcgcg cgggatcttc gcgaacggca attgttgcgg
    11701 cctgccgatc gcgggaggag tgattctctc ttcaggggat ataacagatg cgacagggcc
    11761 caatgatgtt gacaataaag gggaagctgt gggcggtctg cctgatgccg acctgaatct
    11821 tctcgtcggt ggctcgggca gcaaggacgc ggcaatcctg gagttcgata tcatttcaac
    11881 aaacgcttgt ttgctgcaat tcagatatgt ttacacttct gaggagtatc cggagttttg
    11941 tgggtcaggg ttttgcgatc cctttgccgt ttttgttaca acaaatcgtg tgggtgcgaa
    12001 ctggattaat actataagca acaa
//
LOCUS       DAOQWR010000075        12020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5793_length_12020_cov_5.837108, whole genome shotgun sequence.
ACCESSION   DAOQWR010000075 DAOQWR010000000
VERSION     DAOQWR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_12020_cov_5.837108"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKK36_07215"
     CDS             complement(<1..613)
                     /locus_tag="PKK36_07215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002957136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNR94383.1"
                     /translation="MRSDKVKTGFERAPHRSLMRATGLTAEDMKKPFIAVCNSYTDVV
                     PGHVHMAKVGKIVKDAIRKAGGTPMEFNTIAICDGIAMGHTGMKYSLPSRELIADCVE
                     SMIEAHQFDAMVCLPNCDKIVPGMLIGAVRCNIPTIFASGGPMRAGKMPDGRTVDLIS
                     VFEAVGQLKTGKISEDQLEEMSCYACPGAGSCSGSSAAISVCGS"
     gene            complement(760..1392)
                     /locus_tag="PKK36_07220"
     CDS             complement(760..1392)
                     /locus_tag="PKK36_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNR94384.1"
                     /translation="MDPIIHALDVIGTAVFAVTGALAAGRKRMDVFGVVVLGCVTALG
                     GGTMRDLILGIRPVFWITETPYLAVAVLASAGTYLLGRRFRFPDGLLVHLDAVGLAVF
                     TVVGFQRGLHTTHNPFIAIVMGMTTGVAGGMIRDILSAEMPLILQREIYASASLCGAA
                     LLAALSQLKVPPLAAVPASAAVTLAIRLVAVRWKLSLPVFSLREYPGDGK"
     gene            1521..1597
                     /locus_tag="PKK36_07225"
     tRNA            1521..1597
                     /locus_tag="PKK36_07225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1555..1557,aa:Val,seq:gac)
     gene            1627..3381
                     /gene="thrS"
                     /locus_tag="PKK36_07230"
     CDS             1627..3381
                     /gene="thrS"
                     /locus_tag="PKK36_07230"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNR94385.1"
                     /translation="MKPEKDLELHRMRHSAAHVMAAAVCRLYEDVQLDIGPDTDEGFY
                     YDFDLEHRLTPEDFPQIEAEMQKIVKEDIPFERIEVPREEALKILQDRKQRYKVERLA
                     DIPEGDTISFYKCGDFMDLCRGPHVESTGKIKAFKLLSVAGSYYRGRETNPMLQRLYA
                     TAFLSEKEVRNYLRQLEEAAKRDHRKLGRELDLFSFQEKVGPGLVHWHPKGARIRVAI
                     EDYWRKKHLSSGYELIFTPHIGQAQLWETSGHLGFYRESMYAPMDVDGKEYFVKPMNC
                     PFHIQIFNSRKRSYRELPLRWAELGTVYRYEKAGVLHGLFRVRGFTQDDAHIYCTPDQ
                     MESEIRNAVRFAGQMWRDFGFTEISAFVSTRPDKAVGDPARWDDATRALEAALKEEKI
                     AYEVDQGGGAFYGPKIDMKVRDAIGREWQVSTIQFDFNEPERFDMSFIGEDGREHRPY
                     MIHRALLGSIERFFGILVEHYAGAFPLWLAPEQVRVLPLTDKQMPYAEKVAEQLAAAD
                     FRATVDHRQEKIGAKIRAAQMEKIPYMLVVGPQEEEKGVVALRSRLGGDQGAVRMEDL
                     MARLDAEIASKGLNSTEE"
     gene            3423..3995
                     /gene="infC"
                     /locus_tag="PKK36_07235"
     CDS             3423..3995
                     /gene="infC"
                     /locus_tag="PKK36_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNR94386.1"
                     /translation="MKDRTRRNHAIRVPEVRCIDQDGNQIGVIATREALKLSQEAGMD
                     LVEISPMAKPPVCRIMDYGKFKYDKEKRQRESRKNQVSGKLKEIKFHANVGEHDYDFK
                     LRHARDFLEEGHRVKFSLFFRGRENAHREIGFEVMNRLAGDLNDIGIVEQAPRMMGRT
                     LSMQINPNPKARSATRKRQEHEGPAAGAAE"
     gene            4064..4138
                     /locus_tag="PKK36_07240"
     tRNA            4064..4138
                     /locus_tag="PKK36_07240"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4097..4099,aa:Glu,seq:ttc)
     gene            4220..5287
                     /locus_tag="PKK36_07245"
     CDS             4220..5287
                     /locus_tag="PKK36_07245"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNR94387.1"
                     /translation="MSKTYNIAVIGGDGTGPEVVREAVKVLQASAAKHQFKLNLTDYD
                     FGGARYLQTGEVLPASAVSDMKKHNAILLGAIGHPDVKPGILEKGILLKLRFELDQYI
                     NLRPVKLFPGVEGPLKDKGPEHIDYVVVRENSGDAYTGTGGVTMKDTPHEVAVQTAVY
                     SRFQVDRCLRYAFEYARQRNKKKTLALCGKTNVLTFLYDLWERAFHEMGDREFKDIKR
                     EYYHVDATCMWMVKNPEWFDVIVTGNMFGDIITDLGAITQGGMGIAAGGNINPEGVSM
                     FEPIGGSAPKYTGKNVINPLAAICAGGMMLDTLGEKKAAAEIEEAVMFVTGKKLKSLG
                     AGKMGYSTSEVGDLVAKQIAG"
     gene            5331..6344
                     /locus_tag="PKK36_07250"
     CDS             5331..6344
                     /locus_tag="PKK36_07250"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNR94388.1"
                     /translation="MKKYNVCVVGAGAVGEEMVKMIQRHKFPVKKLTVLARSARRQKI
                     GGRVYDVKETTMDAFNGMDIAFFAGTEGEKGASQVYGWEAVKRGCVVIDNGDDFRMDK
                     RVPLVIPEVNPQALKKHKGLIANPNCSTIIALMALAPIQKAAGIKRMVVCTYQAVSGS
                     GAAAVRELEDQVRAWAAGKKMKASVYPHPIAFNVLAQIGSVKDIPGVTTEEAKLRRET
                     HKILGDPSIRVSTTCVRVPVFNGHSEAIHLELKKPLSVAKARQVLAKGEGLKVVDDME
                     KSVYPMPTVASGSEITYVGRIRKDDSVKNGLALFVSGDNLWKGAAQNAIQIAEKMIEM
                     KLI"
     gene            complement(6749..7306)
                     /locus_tag="PKK36_07255"
     CDS             complement(6749..7306)
                     /locus_tag="PKK36_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNR94389.1"
                     /translation="MTQDVVPAERIQKAIYLLRGQKVMLDRDLASLYGVETRALNQAV
                     KRQRKRFPDDFMFELTRDEIRNISQTVTCLSSLKHARSAYVFTEQGVAMLSSVLNSDR
                     AVQVNIAIMRAFVQLRQLLATHADLARKLEELEKKYDAQFRGVFDAIRRLMAPPAAPR
                     KAIGFHVREKQGVYRVRKNKGAVVL"
     gene            7527..8489
                     /locus_tag="PKK36_07260"
     CDS             7527..8489
                     /locus_tag="PKK36_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR94390.1"
                     /translation="MAKDRKLVSVVTGGSGFLGSHLCDYLLGKGHQVIAIDNLITGNV
                     TNIEHLAGNENFQFIKHDVTNYITVPGDVDYIFHFASPASPIDYLELPIQTLKVGSLG
                     THKALGLARAKNATFFLASTSETYGDPLVHPQPETYWGNVNPVGPRGVYDEAKRFAEA
                     MTMAYHRYHGIDTRIVRIFNTYGPRMRPNDGRVVPTFIMQALKGEPLTVFGEGDQTRS
                     FCYVSDLIEGIYRLAMSRECDPTNIGNPNEMTVLQFAEVIIKLCNSSSKIVNKPLPTD
                     DPKVRRPNIDKARKILGWEPVMNLEDGLQSTIAYFKNLLEKGLI"
     gene            8529..9017
                     /locus_tag="PKK36_07265"
     CDS             8529..9017
                     /locus_tag="PKK36_07265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNR94391.1"
                     /translation="MQLRYTAKDGIEQVFILPDRPVTIGRGLNADLVLKDEKASRLHC
                     AVESFEGECLVRDLKSKNGLLVNGERVETAYVTPGDTFQIGGTVFLLEELFTPGSETA
                     LQEVQDEMEHGKGYGTIMRELVKEVEAAPEKMSDTERITLRPPPRKPKKLADTERIPL
                     DE"
     gene            complement(9018..9497)
                     /locus_tag="PKK36_07270"
     CDS             complement(9018..9497)
                     /locus_tag="PKK36_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNR94392.1"
                     /translation="MKKRALKIGVLGPHRCTPEELEQGRQAGRKIAEAGAILVCGGLD
                     GMMAAAAQGAKEAGGQTLGILPGDSADDANEFIDIALPTGLGLYRNALIAKVCDSVIA
                     IGGYYGTLSEIAFALRAGKRVVTLGSWKIGRHGDSDAGIVEAATPEEAVALALSPGE"
     gene            complement(9494..10993)
                     /locus_tag="PKK36_07275"
     CDS             complement(9494..10993)
                     /locus_tag="PKK36_07275"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNR94393.1"
                     /translation="MTAYTSIGRLAERAGETVCLRGWLFGRRSGGRVIFLLVRDGTGV
                     CQCVVEAASASAFAAAVKLPHECSLRVRGEVRLDDRAAGGVEVAAETVELIQGQEEYP
                     ISRKAHGIDFLLNHRHLWLRSPRQCAILKIRHTLIREIRRFFDDRGFTLIDTPILTPG
                     AAEGAGSLFPVEYFGEEVYLAQTGQLYLESAAMALGKVYCFGPTFRAEKSKTRRHLTE
                     FWMVEPEIAFAELPDLMALAEEMICLLVSAVLEGHAQDLVLLERDTAPLRKIVPPFPR
                     LTYTQAVDLLHSREMKDKVEGDIGRACASLQENMLKLSALEAELPQAGKKWKQDKLTG
                     EIQYVRETVREQEAFIEQSRERLKGAADFRWGSDLGGDEETLLCRYFDKPVIVTEYPR
                     EAKAFYMKRSPTDPRTVLNLDVLAPEGYGEIIGGSQREDDLDELMRRMKDEKMDPAPY
                     EWYLDLRRYGSVPHGGYGLGIERTLAWICGLKHIREAIPFPRLMGRIYP"
     gene            11136..>12020
                     /locus_tag="PKK36_07280"
     CDS             11136..>12020
                     /locus_tag="PKK36_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94394.1"
                     /translation="MTTDTLPGHEQQPEGITCSSCGRFVGPLNKCPHCGARVNKRLSV
                     HVTRYAAVLLATAGLGLLYLYSVNREIPTIRIGDIKETMNFAYVTIEGDVTSEPRVFK
                     QAGRVRSIRFDIDDGTGEIPVTAYSSQAQALVDAGKVPRLGDRAKISGSLSVSADNNV
                     LMRLQSPAQLTLTEPELQVTAIADISQDITDPSVLIEGVITAVREPPANSKAPWTIVV
                     KDDTGEIPVAFWQDIHAEIEEPHLLEAGAKVSVRAGIGSYRGNLQLRMAGGKDIEFTG
                     RADIRSMVAGPGDHAADVR"
BASE COUNT         2640 a         3613 c         3449 g         2318 t
ORIGIN      
        1 ccgaaccgca taccgaaatt gccgcgctcg atcccgagca ggagcccgcg ccgggacagg
       61 catagcagga catttcttcg agctggtcct cgctgatttt cccggtcttg agctgaccaa
      121 ccgcttcaaa cacgctgatc aggtccaccg tgcgcccgtc gggcattttg ccggcgcgca
      181 tcgggccgcc gctcgcaaag atcgtaggaa tgttgcagcg caccgcgccg atcagcatgc
      241 cgggcacaat cttgtcgcag ttcggcaggc agaccattgc gtcaaactgg tgggcctcga
      301 tcatggattc aacgcagtcg gcgatcagct cgcggctggg cagtgaatac ttcatgcccg
      361 tgtgccccat cgcaatgccg tcgcagatgg cgatggtgtt gaactccatc ggcgttccgc
      421 cggccttgcg gatcgcgtcc ttcacgatct ttccgacctt cgccatgtga acatggcccg
      481 gaacaacatc cgtgtaggag ttgcagacgg cgatgaaggg cttcttcatg tcctcggcgg
      541 tgaggccggt ggcccgcatc aggctgcggt ggggggcgcg ttcaaatccg gttttaacct
      601 tgtcgcttct catgatgctg tcctttcaaa aaattctgcc gtattgtacg ccttatttcc
      661 gccgaaccca aacgaaaatt tccgcggaca ggcggctgcc atgaaacagt actaaatcag
      721 tactatataa aaaccggggt tcgggggatc tcccggtcat catttcccgt ctccgggata
      781 ttccctgaga ctgaaaaccg ggagcgaaag tttccagcgc accgcaacca gccggatggc
      841 cagcgtcaca gcggccgaag caggcacagc ggccaggggc ggaaccttga gctgcgacag
      901 cgccgcaagc agggccgcgc cgcacaggct cgcggaggca tagatttccc gctgcaggat
      961 cagcggcatt tccgccgaca gaatgtcccg gatcataccc cccgccacgc ccgtggtcat
     1021 gcccatgaca atggcaatga agggattatg ggtggtgtga aggcccctct ggaacccgac
     1081 aacagtgaag acagccagtc caaccgcatc cagatgcaca agcaggccgt ccggaaaccg
     1141 gaatcggcgg cccagcagat aggtccccgc cgacgccagg accgcgacag caagataagg
     1201 tgtttccgtg atccagaaaa cgggccggat tccgagaatc aggtcccgca ttgttccgcc
     1261 ccccagcgcc gtgacacagc ccagcaccac cacgccaaac acgtccattc gttttcttcc
     1321 ggccgccagc gcgccggtga ccgcaaacac cgccgttccg atcacatcca gcgcatgtat
     1381 gattggatcc atatgcccgt tccgaatgcg gccgtcctca accgcaaacg gagcgtagca
     1441 ggcgggcagt tttttacaag gcttcattga catcgcggaa ccggaaacct atattcacac
     1501 cgattttgat gaaaaaacgc gggcgattag ctcagttggt tagagcgctt gcttgacatg
     1561 caagaggtca caagttcgag tcttgtatcg cccaccatta cagaaggaag cagacgcagt
     1621 acaggcatga agcccgaaaa ggatttagag ttgcacagga tgcgccacag cgcggcgcat
     1681 gtcatggcgg cggcggtctg cagactttat gaagatgtcc agctggacat cggtcccgac
     1741 accgacgaag gcttctacta cgatttcgat ctggagcacc gcctgacccc cgaggatttc
     1801 ccgcagatcg aagctgaaat gcagaagatc gtgaaggagg acatcccctt tgagcggatt
     1861 gaagtgccgc gcgaagaggc tctgaaaatc ctgcaggacc gcaagcagcg ctacaaggtt
     1921 gagcgtctgg ccgacatccc cgagggcgac acgatcagct tctacaaatg cggtgatttc
     1981 atggacctct gccgcgggcc ccatgtggag agcaccggga aaatcaaggc gttcaagctg
     2041 ctttccgtgg ccggctcgta ttaccgcggg cgggaaacca atccgatgct gcagcggctc
     2101 tacgccacgg cgtttctgtc tgaaaaggaa gtccgcaatt atctgcgcca gctggaggaa
     2161 gccgccaagc gggatcatcg caagctcggc cgcgaactcg acctgttctc tttccaggag
     2221 aaggtcgggc cgggactggt gcactggcac cccaaaggcg cgcgcatccg cgtggccatt
     2281 gaggactact ggcgcaaaaa gcacctgtct tcgggttatg aactgatctt caccccgcac
     2341 atcggccagg cacagctgtg ggaaacgtcc gggcacctcg gcttctaccg cgaaagcatg
     2401 tacgcgccga tggatgtgga cggaaaagag tatttcgtca agccgatgaa ctgccctttc
     2461 catattcaga tattcaattc gcgcaagcgc tcctaccgcg agctgcccct gcgctgggcg
     2521 gaactcggca cggtgtaccg ctatgagaag gccggcgtgc tccacggcct cttccgggtg
     2581 cgcgggttca cgcaggacga cgcgcacatc tactgcaccc cggaccagat ggagtccgaa
     2641 atccgcaacg ccgtccggtt cgccgggcag atgtggcggg acttcggatt caccgaaatt
     2701 tcggcttttg tttccacccg ccccgacaag gctgtcggcg atcccgcgcg gtgggatgac
     2761 gccacccgcg ctctcgaagc cgcattgaag gaggaaaaaa tcgcctacga agtggatcag
     2821 ggcggcggcg cgttctacgg gcccaagatc gacatgaagg tccgcgacgc catcggccgt
     2881 gaatggcagg tcagcaccat ccagtttgac ttcaacgaac ctgagcgctt cgacatgtcc
     2941 ttcatcggag aagacggacg ggagcaccgg ccctacatga tccaccgcgc gctgctggga
     3001 agcatcgagc gctttttcgg aatcctggtg gagcattacg ccggggcctt cccgctctgg
     3061 ctcgcgcccg agcaggtgcg cgtgctgccg ctgacggaca agcagatgcc ctatgccgaa
     3121 aaggtggccg aacaactcgc cgccgcggat ttccgggcga ccgtggatca ccgccaggag
     3181 aaaatcggcg ccaaaatccg cgccgcgcaa atggagaaga tcccctatat gctcgtggtc
     3241 ggcccgcagg aggaggaaaa gggcgttgtg gcccttcgtt cccgtctggg cggcgatcag
     3301 ggcgcagtcc gcatggaaga tttgatggcc cggctggatg ccgaaatcgc gtccaaaggc
     3361 ctgaacagca cggaagaata aaccccaacc cggaggctcc tatcagcaaa tacgacagcg
     3421 caatgaaaga tcgtacacgg cggaaccacg ccattcgcgt gccggaagtc cgttgtattg
     3481 atcaggacgg aaaccagatc ggcgtcatcg ccacccgcga agcgctcaag ctctcccagg
     3541 aggcgggaat ggatctggtg gaaatttccc cgatggccaa gccccctgtc tgccgcatca
     3601 tggactacgg caaattcaaa tacgacaagg aaaagcgcca gcgggagtcc aggaaaaacc
     3661 aggtttcagg caaactcaag gaaatcaaat tccatgccaa cgtgggggag catgactacg
     3721 acttcaaact gcgccatgcg cgcgacttcc ttgaagaggg acaccgggtc aagttctccc
     3781 tgttcttccg cggccgcgaa aacgcgcacc gggaaatcgg ctttgaggtg atgaaccggc
     3841 tggccggtga cttgaacgat atcggcattg tcgagcaggc cccccggatg atgggccgca
     3901 cactctccat gcagatcaat ccgaatccga aggcccgctc ggccacgcga aaacgccagg
     3961 agcatgaagg cccggcggcc ggcgcggcgg agtgagaaaa attccttttt tgggttgcgt
     4021 gacgggtgtt ctctgttaaa ttgtcccgca cgttggttgg agcgacccca tcgtctagtg
     4081 gttaggacat cgggttttca tcccggcaac acgggttcga gtcccgttgg ggtcgccatt
     4141 ttccagacgt aatgattatt tgcggagcgt ccgtcggcac gggcgtattt ttttaccggc
     4201 aaatgaaaag gaaaaggaaa tgagcaaaac gtacaacatc gccgtgatcg gcggcgacgg
     4261 caccgggccg gaagtggtcc gcgaggccgt caaggtgctg caggcctccg cagccaaaca
     4321 ccagttcaaa ctgaacctga ccgactatga tttcggcggc gcacgctatc tccagaccgg
     4381 cgaggtactc cccgcttcgg ccgtgtccga catgaaaaag cataacgcca ttctgctcgg
     4441 cgccatcggc catcccgatg tgaagcccgg catccttgag aagggcatcc tgcttaaact
     4501 gcgttttgaa ctggaccagt acatcaacct gcggcccgtc aagctgttcc ccggcgtgga
     4561 gggcccgctg aaggacaagg gccccgagca tattgactat gtggtggtgc gcgaaaacag
     4621 cggcgacgcc tacaccggca ccggcggcgt caccatgaag gacacccccc acgaagtggc
     4681 cgtccagacc gccgtctatt cccgcttcca ggtggaccgc tgcctgcgct acgcgtttga
     4741 atatgcccgg cagcgcaata aaaagaaaac cctcgcgctg tgcgggaaga ccaacgtgct
     4801 cacgttcctc tatgacctgt gggagcgggc gtttcacgag atgggcgaca gggagttcaa
     4861 ggacatcaag cgcgagtact accatgtgga tgcgacctgc atgtggatgg tcaagaatcc
     4921 cgagtggttc gatgtgatcg tgaccggcaa catgttcggc gacatcatca ccgacctggg
     4981 cgccatcacg cagggcggca tgggcatcgc ggccggcggg aacatcaacc ccgaaggcgt
     5041 cagcatgttc gagccgatcg gaggctccgc gcccaaatac accggcaaga acgtgatcaa
     5101 tccgctggcg gccatctgtg cgggcggcat gatgctcgac acgctgggcg aaaagaaggc
     5161 ggccgcggaa atcgaggaag ccgtcatgtt tgtgacaggc aagaagctga agagccttgg
     5221 cgcgggcaag atgggatatt ccacatctga agtcggcgac ctggtggcga agcagattgc
     5281 aggttgaatt ttgcgcggat gcgcatgata ttcagttcgg agtaaataca atgaagaaat
     5341 acaacgtgtg tgtggtcggc gcaggggccg tcggcgaaga aatggtgaag atgattcagc
     5401 ggcacaagtt cccggtcaag aaactgaccg tgcttgcccg cagcgcccgc aggcagaaaa
     5461 tcggcggcag agtctatgac gtgaaggaaa ccacgatgga cgccttcaac ggcatggata
     5521 tcgctttctt cgcgggcacc gagggcgaaa aaggggcatc gcaggtctac ggctgggaag
     5581 ccgtgaagcg aggatgcgtc gtgattgaca acggcgacga tttccgcatg gacaagcgcg
     5641 tcccgctggt cattcccgaa gtgaatcccc aggcgctgaa aaaacacaaa ggcctcatcg
     5701 ccaacccgaa ctgctctacc atcatcgcgc tgatggcgct ggccccgatt cagaaggctg
     5761 cgggcatcaa gcgcatggtc gtctgcacct atcaggccgt gtccggcagc ggcgccgccg
     5821 ccgtgcgcga actcgaagac caggtgcgcg catgggccgc gggcaaaaaa atgaaggcct
     5881 cggtctatcc ccaccccatc gcgttcaacg tgctggcgca aatcggtagc gtgaaggata
     5941 ttcccggtgt caccaccgag gaagccaagc tccgccgcga aacccacaaa atcctcggcg
     6001 acccgtccat ccgtgtctcc accacctgcg tgcgcgtacc ggtgttcaac ggacattcgg
     6061 aagccattca tctggagctg aaaaagcccc tcagcgtggc gaaggcccgc caggtgctgg
     6121 ccaaaggcga aggcctcaag gtcgtggacg acatggagaa atccgtttat cctatgccca
     6181 ccgtggcctc aggctcggaa atcacctatg tcgggcgcat ccgcaaggac gacagcgtga
     6241 aaaacggcct ggcgctcttc gtttccggcg acaacctgtg gaagggcgcc gcgcagaacg
     6301 cgatccagat cgccgaaaaa atgatcgaga tgaagctcat ctgatcgttc cttcagaata
     6361 aaaccaacag ccccgggcaa aacccggggc tgtttttttt cggccccaat ttggggatgt
     6421 tcaaagtttt gcgagttctg cgcggaaccg catgcccaga ccgactgatt cttcagcgcg
     6481 caaatagtct ttcagttcac cggcaatgag ttcgtcaaac agcgtgaata cccccgagct
     6541 cccccgccca ggtatccaga cattctttgc cctctttaat catttgctat aactatattt
     6601 tatttatagt ttctattaat gttaatttct cgcgaattga tgactgaagg tttttgcata
     6661 tagagcaggc gaccagtata ctgacctgcc gcggcagatg aaaaacggga aatcggaaca
     6721 tcattttcga tcggctgaaa acatcatttc ataggaccac tgcaccctta ttctttctga
     6781 ctcggtaaac gccctgtttt tcgcgcacat gaaagcctat ggccttgcgt ggtgccgccg
     6841 gcggagccat aagccggcga atggcatcga acactccccg gaactgtgca tcgtattttt
     6901 tctcaagttc ctccagtttt cgggccagat ccgcatgtgt ggccagaagc tgcctcaact
     6961 ggacaaacgc ccgcatgatg gcaatgttta cctgaactgc gcggtcactg tttaacacgc
     7021 tggaaagcat agcaacgccc tgttctgtga aaacataagc gcttctcgca tgtttcaagg
     7081 aagacagaca tgtcacagtt tgtgatatgt tccgaatttc atccctcgtc agttcaaaca
     7141 tgaagtcatc cgggaaccgc ttgcgctggc gtttaacggc ctgattgagt gcacgcgtct
     7201 caactccata gagcgacgcc aaatcccggt caagcatgac cttttggccg cgaagcaaat
     7261 agatcgcttt ctggattctc tctgcgggca caacatcctg tgtcatattg cctccggtgc
     7321 tgactgaaat cacatgctat aaccgcgtag ataatttttc aatccaactc tgcaattttg
     7381 agggtttttg attttttggg gccatttttg ttcatttttc gagtattttt ggccgttttt
     7441 tgggcattcc ggtttttgtt cacgggcgct gtacttttcc gggttatctg ctagtctgaa
     7501 gactcccatt tggaaggacg ggcattatgg caaaggatcg caaactggtt tcggtggtga
     7561 cgggcggatc gggtttcctc gggtcgcacc tgtgcgatta tcttctgggc aagggccacc
     7621 aggtgatcgc cattgacaat ctcatcaccg gcaatgtaac caacatcgag catctcgcgg
     7681 gcaatgagaa tttccagttc atcaagcacg atgtgacgaa ttatatcacc gtcccgggcg
     7741 atgttgacta catattccat ttcgcctcgc cggcgagtcc gattgactat ctggaacttc
     7801 cgatccagac gctgaaggtg ggatcgctgg gcacgcacaa agccctcgga ctggcgcgcg
     7861 cgaagaatgc cactttcttt ctggcgtcca cgtcagaaac ctacggtgac ccgctggtcc
     7921 atccgcagcc ggagacctac tggggcaatg tgaacccggt cggcccgcgc ggcgtgtacg
     7981 acgaggccaa gcgctttgcc gaggccatga cgatggccta tcaccgctat cacggaatag
     8041 acacgcgcat cgtccgcatc ttcaacacct acggcccgcg catgcggccg aacgacggac
     8101 gcgtggtgcc cacgtttatc atgcaggcgc tcaaggggga accgctcacc gttttcggcg
     8161 agggcgacca gacccgaagc ttctgctatg tgtccgacct gattgagggc atctaccggc
     8221 tggccatgag cagggagtgc gacccgacca atatcggcaa cccgaacgag atgacggtcc
     8281 tgcagtttgc ggaagtaatc atcaagcttt gcaacagctc atcgaagatc gtcaacaagc
     8341 cgctccccac ggatgacccg aaggtgcgcc gccccaatat tgacaaggcc cggaaaattc
     8401 tgggatggga accggtgatg aatctggaag acggcctgca gtccaccatc gcctatttta
     8461 agaatcttct ggagaagggg ctgatataaa cgcttgatct ccgaaatcct ttgaaccagt
     8521 atggccccat gcaattgaga tatacagcta aagacggcat cgagcaggtt tttattctgc
     8581 cggacagacc tgtcacgatc ggccgcgggc tgaacgccga cctggtgctc aaggatgaaa
     8641 aggcgtcgcg gctgcattgc gccgtggagt ctttcgaggg cgagtgcctc gtgcgcgacc
     8701 tcaagtccaa aaacgggctc ctcgtcaacg gagagcgcgt ggaaaccgcc tacgtaaccc
     8761 ccggcgacac ctttcagatc ggcgggaccg tgtttctgct tgaagaactg ttcacccccg
     8821 gatcggagac cgccctgcag gaagtgcagg acgaaatgga gcacggcaag ggctacggca
     8881 ccatcatgcg ggaactcgtg aaggaagttg aagcggcgcc ggagaaaatg tcggacacgg
     8941 agcgaatcac actgcggccg ccccccagga agccgaagaa gctggccgac accgagcgca
     9001 tcccgctgga cgaatagtta ttcccccggg cttaaggcca gagcaaccgc ctcttccggt
     9061 gtcgcagcct ccacaatacc ggcatcagaa tccccatggc gtccgatttt ccatgatccc
     9121 agagtcacaa ctctttttcc ggcgcgcagg gcaaacgcga tttctgacag ggtgccgtag
     9181 tatccgccga tggcgatcac tgagtcacag accttcgcaa tcagcgcgtt gcggtagagt
     9241 cccaggcccg tgggcagagc aatgtcaatg aactcgttgg catcatccgc cgaatcgccc
     9301 ggaaggattc cgagcgtctg gccgcccgcc tctttcgcgc cctgcgcggc ggcggccatc
     9361 attccgtcca atccgccgca gacgagaatg gcgccggctt cggcgatttt gcggcccgcc
     9421 tgccgcccct gctccagttc ttccggtgtg cacctgtgcg ggcccagcac gccaatcttc
     9481 aaggcgcgtt tcttcacgga tatatcctcc ccatcagcct cgggaacggg atggcttcgc
     9541 gaatgtgctt cagtccgcag atccacgcca gtgtgcgctc gatgcccagg ccgtagccgc
     9601 cgtgcggcac ggagccgtag cggcgcaggt ccagatacca ctcatagggc gcgggatcca
     9661 ttttctcgtc cttcatgcgc cgcatcagtt catccaggtc atcttcgcgc tggctgccgc
     9721 cgatgatttc gccatagcct tcaggcgcga ggacatcgag attcagaacc gtgcgcgggt
     9781 ccgtcggcga gcgcttcata tagaatgcct tggcctcgcg cggatattcc gtgacaatca
     9841 cgggtttgtc aaaatagcgg cagagcagcg tttcctcgtc gccgcccaga tcggaacccc
     9901 accggaaatc ggccgcgcct ttcaggcgtt cccgggactg ttcgataaag gcttcctgtt
     9961 cgcgtacggt ttcacgcacg tactgaatct caccggtcag cttgtcctgc ttccatttct
    10021 tcccggcctg cgggagttcc gcctccagcg ccgacagttt cagcatgttt tcctgaaggc
    10081 tagcacacgc ccgcccgatg tcgccttcca ccttatcctt catctcacgg ctgtgaagga
    10141 ggtccaccgc ctgcgtgtag gtgagacgcg gaaacggcgg cacaattttt ctcagcggcg
    10201 ccgtatcgcg ctccagcagg accagatcct gtgcatgccc ctccagcacg gccgacacca
    10261 gaaggcagat catctcttcg gccagcgcca tcagatccgg aagctccgca aacgcgattt
    10321 cgggctccac catccagaat tcggtcagat gcctgcgggt tttggatttt tccgcccgga
    10381 aggtggggcc gaagcagtac actttgccca gcgccattgc cgcgctttcc agataaagct
    10441 gtccggtctg cgccagatat acctcttcgc cgaaatactc caccgggaag aggctccccg
    10501 cgccctcggc cgcgccgggc gtcagtatgg gcgtgtcaat cagggtgaag ccgcggtcat
    10561 caaaaaagcg ccggatctca cgaatcagcg tgtgccggat tttcagaatg gcgcactggc
    10621 gcggcgagcg gagccacaag tgccggtgat taagcagaaa atctatgcca tgggccttgc
    10681 gggaaatcgg atactcctcc tgtccctgaa tcagctccac ggtttcggcg gcaacttcca
    10741 ccccgccggc cgcgcggtcg tccaggcgca cttcgccgcg gacccgcagc gagcactcat
    10801 gcggaagctt taccgcggcg gcaaaggcgg aggcggaggc ggcctcgacc acgcactggc
    10861 agaccccggt gccgtcacgc accagcagga aaatcacccg gccgccggat cgccggccaa
    10921 agagccagcc ccgcagacaa accgtttccc ctgctctttc cgccagccgg ccaatagatg
    10981 tgtatgcggt cataaggtct ttccagtcat tcacaggccg gaaacctaca gaaattctgt
    11041 atttaagact atccaaaaaa ggcccgcttc ggtagtcttg tcaggctgta tcggattttt
    11101 aggtattggc gaaacaaaca gaaaggaaca gtcccatgac tacagacaca ctccccggtc
    11161 atgagcagca gcccgaagga atcacctgct ccagctgcgg ccgctttgtc ggcccgctga
    11221 acaaatgccc ccactgcggc gcacgcgtca acaagcgttt gagcgtgcat gtcacgcgtt
    11281 atgccgccgt tctgctggcc accgccggcc tgggcctgct ctatctgtat tccgtgaaca
    11341 gggaaatccc gaccatccgc atcggcgaca tcaaggaaac gatgaatttc gcctatgtga
    11401 ccatcgaggg agatgtgacc agtgagccgc gcgtgttcaa gcaggccgga cgcgtccgca
    11461 gcatccgctt tgatattgat gacggaaccg gggaaatccc cgtgaccgcc tattccagcc
    11521 aggcgcaggc gctggtggat gccggcaagg tcccccggct cggagaccgg gccaaaatca
    11581 gcggcagcct gagtgtttcg gctgacaaca acgtgctcat gcggcttcag tcgccggcgc
    11641 agctgacact cacggaaccg gaactgcagg tcaccgcgat agcggatatc agccaggaca
    11701 tcacggatcc ctccgttctc atcgagggcg tgatcacagc cgtccgggag ccgccggcca
    11761 attcaaaggc tccgtggacc attgtggtaa aggacgacac cggcgaaatc ccggttgcgt
    11821 tctggcagga catccacgcc gaaattgaag agcctcatct tctggaagcc ggcgcgaagg
    11881 tcagcgtccg cgcggggatt gggagctacc gaggcaacct ccagctgcgc atggcaggcg
    11941 gcaaggacat agaattcaca ggcagggcgg acatccgcag catggtcgcc gggcccggcg
    12001 accatgctgc ggatgtccgc
//
LOCUS       DAOQWR010000076        11986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5818_length_11986_cov_5.116503, whole genome shotgun sequence.
ACCESSION   DAOQWR010000076 DAOQWR010000000
VERSION     DAOQWR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_11986_cov_5.116503"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1302
                     /locus_tag="PKK36_07285"
     CDS             328..1302
                     /locus_tag="PKK36_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNR94395.1"
                     /translation="MKRKLLVVSFLSLSVALTAVPSFALWGKAETKTSDSDAQMNLGE
                     YKGLKHAIGCKDFDNEAGWHGEWQLGENLSIMLESALYDTGRFVLVEREKLKDVIAEQ
                     DLVSSGRAAQTKKIAQTGKIRPAKYLASGAVTTVEDNQSGGHGGISIKGISVGMKKNE
                     AQITIIAKLIDTTTSEIVAKKSITGKAGGTGLDLGVYKGGVGVDLGGFKKTPIGQAAQ
                     DCINQAAKFFATAMEEFPFEGSVIKVSGSQVIINRGSEFGVETGQELVMSEEGETLID
                     PDTGAILGQEDGKVIGKIRVAKVNEKISYCDIVSGEKNPATGTLVRAE"
     gene            complement(1375..2565)
                     /locus_tag="PKK36_07290"
     CDS             complement(1375..2565)
                     /locus_tag="PKK36_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94396.1"
                     /translation="MRKLLLTLWMCSAAVCFGRWYFEAGPWMRSDMEMKVNDGSRAAE
                     QGVRAAVPGTRGGRASIPAPAAEDDGTAQVLRQFDNGYVGPSAWAWAQTLGISQYFGY
                     DSASQYDAAASTLAFTRSASASDTQRRTVTQVSSGSAGWTGSRDLDASGFLITLGRML
                     MSEARFGAGLQVQLGWLDGIEASFHNRTAWEQQVKWTTYESQVSRAQSYNYVYDTLGA
                     PVPAAPYSMTDPGGVGPMIADRPSAIVPGAGSEESFSERAVRRQTAMARSRVDMDAEI
                     DALVLVFGPRMIFRPADRLLFLVQCGATLNLLDASFSRTETFAWEDGRVIQSWSDRRD
                     RQQWLWGAGVSAGIQYDVNESLYLFVSAGYDWVESHEFKFGSSRIEVDLSGVQASAGV
                     GIRF"
     gene            complement(2578..11754)
                     /locus_tag="PKK36_07295"
     CDS             complement(2578..11754)
                     /locus_tag="PKK36_07295"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HNR94397.1"
                     /translation="MLSRRFPFTRLRFSFVAALVAIAMWLSAPGGVAEAAGSWTNVIG
                     LPMQTVNHCAAVLNDKLYVLGGGTIPNSYSSTNAFRLDGTNWTSIPWAPVGGFGGLIS
                     AGAATLNGTLYLIGGHNGSSERTNVYSFNGTNWTVAPSLPAARQYLAACTFQSKIYAI
                     GGRFGVSAQTNVYCFDGTNWSETVGLPVVNMALSASVHNGRLYAVGGYDGVTPSYTNV
                     YRFDGTNWTAVAGLPVPRSHHSTAEMGGRLYAMGGVYGTVHSNVYCYDGTNWTESPGL
                     PLAKGYLAAAATLGNKIYHTGGSTDMANGVTNVFAWVDPSVKLDQLITFVPVPPQKVS
                     GSVGLAATASSGLPVGFAVGSGPASITGGTNLSFTGVGTVSIVVSQSGDATYNPAPNV
                     TNTFHVYALSAYSGPFAGGNTITITNGNFGTITNVLVGGVASTIEASGANWVRITVPA
                     AGSAGAKDLVIQTSDNGDKTLAAAYTVNPAGDISGGTGSRMWQSVGQALVPGQVSAVG
                     ANNTIYGMDYYGNNLYIGGAFTNAGGSNCYRVARHDGTNWHSMQAGVLRVANVNCIKG
                     SPHGIYSGGYFTNIGGSYNPDGTMNNDGGVNAYAAARFDGTSWNAMGHAPLLGNKTGL
                     YFTVSINGYVNWMEPYKDDTVIAVGYFTNSDYRAAGLNYIAKYDGAGWTNMQGGFRNV
                     PLAAAYDRDRDHLYVGGSFTNHYPTNTSRHIRYIARWDGVNWTNMAQGFGNRVTCMAV
                     NPANGELYIGGWFTNYFDSAGDPHPANYVAKWDPVSQSLTNVGSGFNNWVYALKVAPD
                     GTLYAGGSFTNTYISTEPDKSAPQLAVRRIAMWNGTHWTNMGRGLSDTVLSLTVNTNN
                     NDVYAGGYFRIATQDDGTDTNTWHIASYGAGGVTSTGVDPDTGPITGGFEVVISGSNL
                     GSGDITNVTLCGVSVASIVSQSATQVVVVAAAGDPGMGDVRVYSASYGETVKSDAFTY
                     TGPLFALLGANGAAVASGEAVSEAKGTDFGTINLGDAATNRFAITNAGDAVLTISSWP
                     TNGTGAGAFAIAGMPVTVAAGTISNFSVIFTPVSAGTSTCTVSIVNSSTNTPYLLNLS
                     GHGVKQNQTITFPAIPDQLTTNLVGLAATASSGLAVQFSVGGGSALITGDTNLSFTGT
                     GSVLIVASQIGDANWNPAPNVTNTFNVTKAAAGVYLQDLAQTYDGTARSVTATSDPSG
                     LTIEFTYNGNAWAPTNAGSYAITGVVNDVMYQGSQTGTLVISKGAASVYLLDLAQTYD
                     GTAKSVTATSDPAGLTIEFTYNGNAWAPTNAGSYAVTGTVNDVNWVGSADGTLVVSKG
                     TAGVYLLNLAQTYDGLAKSVTATSDPSGLTIEFSYNGNAWAPTNAGSYAVTGTVNDAN
                     WQGSADGTLVVSKGAAEVYLLDLAQTYDGTARSVAATSDPSGLTIEFTYNGNAWAPTN
                     TGSYAVTGTVNDANWQGTADGTLVVSKGTAGLYLLDLTQMYDGTARSVTATSDPSGLT
                     IEFTYNGNAWAPTNAGSYAITGVVNDVMYQGSQTGTLVISKGAASVYLLDLAQTYDGT
                     AKSVTATSDPAGLTIEFTYNGNAWAPTNAGSYAVTGTVNDVNWVGSADGTLVVSKGTA
                     GVYLLNLAQTYDGLAKSVTATSDPSGLTIEFSYNGNAWAPTNAGSYAVTGTVNDANWQ
                     GSADGTLVVSKGAAEVYLLDLAQTYDGTARSVAATSDPSGLTIEFTYNGNAWAPTNTG
                     SYAVTGTVNDANWQGTADGTLVVSKGAANVYLLDLTQTYDGTAKSASATTMPAGLTVE
                     FTYNGSSTSPSEAGSYAVTGTVNDANWQGEETGTLTIGKAGQTITFSPVAPQKASAIV
                     GLAATGGGSGNPVTFAAGAPGVITDGTNLSYTGVGDVIVVASQAGNADYEAAPDVTNV
                     VKVFSVTPDVGPFAGGHNATISNGYFGTITNVLVGGVAAAIQSSGANWVTLTVPAVGS
                     HGIKDIVIQTSDNGETTLPDAYTVAVRAEDLPLGSLVYDDDWTWSGLPLKWRVVDTNY
                     GGTAGLVTLQATSSLGGRAYHEGAWANQWGSCTLRTWLNSTNFHGGFSPAFSGAVVCT
                     RVPWTTYNSAGAFVSGITTDQVFIASRTELGGDVWASEGEVLDWFSDPGTAAARRSDL
                     SPSPMNYWTRSGMRAMWGVTYYDLAAYYAAVPGGAIAYGPWTGDALGVVPMVNIGGPV
                     EFAVQPDGTYRLYEGPLPQSITFPNPGPQFTTNVVALSATASSGQDVIYSVLSGPASV
                     AGGILTFTGTGDVTVIASHPGNAYWNPAPNLTNAFTVTKATASLALTNLNQIYSGSAR
                     EAGYETVPAGLTVLLTYDGNLWAPTNAGSYAVAGVVDDVMWQGSQTGTLTVAKADQTI
                     AFPPIADQLTTNEVGLAATAASGFDVTFFAGAPPAVIMGGTNLSFTGTGMVSVVASQA
                     GDANWNPAPDVTNTFTVTKAAASLTLTNLNQACDGTARTAGYAVVPSGLTVLLTYNGN
                     VWAPTNVGSYAVTGVVDDVMWQGLATGTLVVVKGDQIITNFLPADGAVFVLGTATSVS
                     AQASSGLAVGFNNLTPEIASLTGTAIAFTNPGAARVQAFQPGDANWNAAVPVVHEWRV
                     GGLITNIMPGAANVGGGIEVLIQGLWLGDGSNITTVELAGVAADIVTQGVHEVTVTAG
                     AAPAAVTGDVFVVSGTGGEMWMSNAFEYLWFDAPVQLDPVDITASNLVARWLLAAGAD
                     KHILEVGLDTNFTAHLPGYEAVDVALAQQYLVAGLTEGTWYAMRLFAWNTNGYSPPSR
                     TVRVPAGTNLPYVTHPPMGGPVTEDAVMQFTLACMFHGSGLIYTAESSDTNIVGVSIA
                     GGVLEIDPRNPGTATITVTATDPATGYSASYSFVIEVVGTVPFSSAFQDREIWNPRFT
                     QRLWVTNDLAIDAIGIRVLFAGLLPGIIVENQTGISVDGRPMIEMEAPFPPDGTMELD
                     IIYICTGAETVSDYPPVVEVQYILPGWRPPLPGEGVPVGVKLVFDAERVLLEFDTTVG
                     MLYAIEYMNNFPAGTWREVSLRLRATANRTQWIDAGPPATLPFEGLRVYRIKAVAE"
BASE COUNT         2363 a         3865 c         3420 g         2338 t
ORIGIN      
        1 caggacaaac agtcgcttcg gaacgcccac cagctctctc cactctgagg ggatcgtaag
       61 gcgtttttta gaatcaaggg agtgagtgta tgtgtttaca aaaacgccca tttgagcgcc
      121 ttcttcaacc acatgactgt ctcttgcttc caccttactg ctctcctgtg acactttatc
      181 ccacaatgcc ccacccagag tcaatttttt atttgaattt ttttcgaata ttttcagtcc
      241 ctaaaaaaag aggccgcacc gagcttgtca atcccctcgc ccccgtgcta gtttcacccc
      301 gatttcggat taaacaaagg agaatccatg aagagaaaat tgctggttgt ttcgttcctg
      361 tccctgtccg tcgccctgac tgccgttccc tctttcgcac tgtggggaaa agccgaaaca
      421 aaaacctcgg acagtgatgc ccagatgaac cttggcgaat acaagggcct caagcatgcc
      481 atcggctgca aagacttcga caacgaggcc ggatggcatg gcgaatggca gctcggggaa
      541 aatctttcca tcatgctcga atccgctctt tatgacaccg gacgctttgt tctggttgag
      601 cgcgaaaagc tgaaggatgt gatcgccgaa caggacctcg tgtcaagcgg ccgcgcggct
      661 cagaccaaga agatcgctca gaccggaaaa atccggccgg caaaatatct ggccagcggc
      721 gcagtgacca ctgttgaaga caaccagtcc ggcggacacg gcggtatcag tatcaagggc
      781 atcagcgtgg gcatgaagaa gaacgaagcc cagatcacca tcatcgccaa gctgatagac
      841 accacgacca gcgaaatcgt cgcgaagaaa agcatcacgg gcaaagccgg cggcacgggc
      901 cttgatcttg gcgtctacaa aggcggcgtg ggcgttgacc tcggcggatt caaaaaaacc
      961 ccgatcgggc aggccgcgca ggactgcatc aaccaggccg caaaattctt tgcgacggcc
     1021 atggaagaat tcccgttcga gggctccgtg attaaagtga gcggcagcca ggtcatcatc
     1081 aatcgcggca gtgaattcgg ggttgaaacc ggacaggaac tggtcatgag cgaagaagga
     1141 gaaaccctga ttgatcccga caccggcgcc atcctcggac aggaagacgg aaaggtcata
     1201 gggaaaatcc gcgtggccaa ggtgaacgaa aagatttcct actgcgatat cgtgagcgga
     1261 gaaaagaatc ccgccactgg aacactggtg cgcgcggaat aaatcagctt ctctgcttcc
     1321 ccaaaaaaag aacatcccgc ccgaaacggc gggatgtttt ttgtccgtct gcccttagaa
     1381 tcttattccc actccggcag aggcctggac tccgctgaga tccacctcta tgcgcgaaga
     1441 gccgaatttg aattcgtgac tctcaaccca gtcgtatccc gcggacacaa aaaggtagag
     1501 gctttcgttc acatcatact ggattccggc ggacacgcct gcgccccaaa gccattgctg
     1561 acggtcacga cgatccgacc agctctggat gacccggccg tcctcccatg caaaggtttc
     1621 ggtgcgcgaa aatgacgcat ccagcagatt cagcgttgcg ccgcactgca ccagaaaaag
     1681 caggcggtct gccggccgaa agatcatgcg cggcccgaaa acaagcacca gggcatcgat
     1741 ctccgcatcc atgtccaccc ggctgcgggc catggccgtc tgccgccgga ctgcacgctc
     1801 actgaacgac tcctctgagc cggcccccgg cacaatggcg ctgggccggt ccgcaatcat
     1861 cgggccgact ccaccaggat ccgtcatgct gtagggcgca gccggaaccg gggcgcccag
     1921 cgtgtcataa acataattat aggactgcgc ccgcgacacc tgcgactcat aggtcgtcca
     1981 tttcacctgc tgctcccagg ccgtccggtt gtgaaaagac gcctcgattc cgtccagcca
     2041 gccgagttga acctgaaggc ccgcgccaaa ccgggcttcc gacatgagca tcctgcccag
     2101 cgtgatcaga aatccggacg catccagatc ccggcttcca gtccagccgg cgcttccgga
     2161 agaaacctgt gtcaccgtgc gacgctgcgt gtctgaagcg gatgcgctgc gcgtgaacgc
     2221 aagcgtgctg gctgccgcat cgtactggct tgcgctgtcg tagccgaaat actggctgat
     2281 gcccagcgtt tgagcccagg cccatgcgga cggaccaaca taaccgttgt caaactgcct
     2341 caacacctgc gccgtgccgt catcctccgc agcgggggcc gggatggagg cccttccgcc
     2401 gcgcgtgccg ggaacagcgg cgcgcacccc ctgttcagcc gcgcgcgagc cgtcattcac
     2461 tttcatttcc atgtcacttc gcatccacgg gcccgcctcg aaataccacc ggccaaaaca
     2521 gacggccgca ctgcacatcc aaagggtcaa taacagtttt ctcacgggac ttctccttta
     2581 ttccgccaca gccttgatcc ggtaaacacg cagcccctca aacggcaggg tggccggcgg
     2641 gcccgcatcg atccattgcg tccggttcgc cgtcgccctc agccgcagcg aaacctctct
     2701 ccaggttccg gccgggaagt tgttcatgta ttcaatcgca tacagcatcc ccaccgttgt
     2761 atcgaactcc agcagcacac gctccgcatc gaagaccagc ttcacgccga cgggaacgcc
     2821 ctcccccggc agcggcggac gccaccccgg aagaatgtac tgcacttcaa ccacaggagg
     2881 ataatcgctg actgtttccg cccccgtgca gatatagatg atgtccagtt ccattgtccc
     2941 gtccggcggg aacggagctt ccatctcgat catcggccga ccgtccaccg atatccccgt
     3001 ctggttctcg actataatac cgggcagcag gccggcaaac agcacacgga tgcctatcgc
     3061 atcaattgcc aggtcattgg tcacccagag ccgctgcgtg aagcggggat tccaaatctc
     3121 acggtcctgg aaggctgaac tgaacggcac tgtccccacc acctcaatga caaaggagta
     3181 gcttgcgctg tatccggtgg ccgggtctgt ggccgttacg gtgatggtcg cggttcccgg
     3241 attgcgagga tcgatttcaa gcacgccgcc ggcgatggat acaccgacaa tgttggtgtc
     3301 gctcgattct gcagtataga tcagccccga tccatggaac atgcaggcca gggtgaactg
     3361 catgacggcg tcctccgtca cggggccgcc catcggagga tgcgtcacat acggcaggtt
     3421 ggttcccgcc ggcacccgga ccgtgcgcga aggcgggctg tatccgtttg tattccacgc
     3481 aaaaagacgc atggcatacc atgtgccctc cgtcaggccg gccacgagat actgctgggc
     3541 cagcgcaaca tccaccgctt catagcccgg cagatgggcg gtgaagttgg tgtcgagccc
     3601 cacttccaga atgtgcttgt ccgcgcccgc ggccagcagc cagcgcgcca caagatttga
     3661 agctgtaata tccacggggt caagctgcac cggcgcatcg aaccagagat actcgaatgc
     3721 gttgctcatc cacatctccc cgcccgtgcc agaaaccaca aatacatcac ccgtcaccgc
     3781 cgcgggagca gcgcccgccg tcacggtcac ttcgtgaacg ccctgcgtca cgatgtcagc
     3841 cgccacaccg gccagctcca ccgtcgtgat attgctcccg tctccaagcc aaagcccctg
     3901 aatcagaact tcgatcccgc cgccgacatt ggccgccccc ggcatgatgt tggtaatgag
     3961 cccgcccacg cgccactcat gcacgaccgg gaccgccgca ttccagttcg catctcccgg
     4021 ctgaaacgcc tgcaccctcg ccgcaccggg attcgtgaat gcgatggccg ttcccgtcag
     4081 actggcaatc tcgggggtca gattgttgaa gccgaccgca agtccgctcg atgcctgggc
     4141 cgacacgctt gtcgcggttc ccaggacaaa cacggcgccg tctgccggaa ggaagtttgt
     4201 aatgatctga tctcccttca ccacaaccag cgtgccggtt gccagaccct gccacatgac
     4261 atcgtccacc acacccgtca ccgcatagct gcccacattg gtgggcgccc acacgtttcc
     4321 attataggtc agcaggaccg tcagaccaga gggaacgacg gcataaccag ccgttcgcgc
     4381 cgtcccgtcg caggcctgat tcaggttcgt cagggtcagc gatgctgccg ccttcgtcac
     4441 cgtgaaggta ttggtcacat ccggcgccgg attccagttt gcatcgcctg cctgcgacgc
     4501 cacaacgctg accatcccgg tgcccgtaaa actcaggttg gttccgccca ttatgaccgc
     4561 tggaggtgcg ccggcgaaga acgtcacgtc aaatccgctc gccgcggttg cagccagtcc
     4621 cacttcattc gtggtcagct gatcggcaat cggcgggaac gctatcgtct gatccgcctt
     4681 cgccaccgtc agtgttcctg tctgggatcc ctgccacatg acatcgtcca ccacgcccgc
     4741 caccgcgtaa cttcccgcgt tggtgggcgc ccacaggttc ccatcataag tcagcagcac
     4801 ggtcagtccg gcgggaactg tttcataccc ggcctcgcgg gcggaaccgc tgtagatctg
     4861 gttcaaattg gtcagcgcca gagaggccgt cgccttggtg accgtgaatg cgttggtcag
     4921 gttcggggcc gggttccagt aggcattgcc cggatgtgat gcaatcaccg tcacatcacc
     4981 tgtgccggta aacgtcagga tgccaccggc cactgaggcc ggcccgctga gcacagaata
     5041 gatcacgtcc tgtccactgg aggccgtggc cgacaaggca acaacattcg tagtgaactg
     5101 cgggcccgga ttcgggaacg tgatgctctg gggcagcggc ccttcataca gccggtaggt
     5161 cccgtccggc tgaactgcga actcgacggg gcctccgatg ttgaccatcg ggaccacccc
     5221 cagcgcatca ccggtccaag gtccgtaagc gattgcccct ccaggcacag cagcgtaata
     5281 ggctgccaaa tcgtaatacg ttacgcccca catggcgcgc atgcccgacc gggtccagta
     5341 gttcatgggc gaaggcgaca ggtcggaacg gcgcgcggcg gccgtgcccg gatcggagaa
     5401 ccagtccagc acctcgcctt cgctggccca cacatctccg cccagttcgg tacgggaggc
     5461 aatgaaaacc tggtccgtgg ttatgccgga aacaaatgcg cctgcagaat tatacgtggt
     5521 ccagggcact cgtgtgcaca ctactgcgcc ggagaacgcg ggggagaacc cgccgtggaa
     5581 attcgtgctg ttcagccagg tccgcaacgt gcacgacccc cactgattgg cccaggctcc
     5641 ttcatgataa gcgcgaccgc ctaggctgct ggtcgcctgc agcgtcacca ggcccgctgt
     5701 gccgccgtag ttggtgtcca ccactctcca tttcagcggc aaaccgctcc aggtccagtc
     5761 atcgtcatat accagggaac ccaggggcag gtcctccgcc cgcacagcca cggtataggc
     5821 atccggcagc gttgtctcgc cgttgtccga ggtctggatg acgatatcct tgatgccatg
     5881 cgaaccgacc gccggcaccg tcagcgtcac ccagttcgcg ccggaggatt ggatcgccgc
     5941 cgcaacgccg ccgacgagca cgttggtgat tgtcccgaag tagccattgg aaatcgttgc
     6001 gttatgcccg cccgcgaacg gaccgacatc cggcgtgacg ctgaagacct tcaccacatt
     6061 cgtcacatcc ggcgcggctt cgtaatcggc attacccgcc tgcgaggcga ctacgatcac
     6121 atcgccgacg cctgtgtagg acaggttcgt accgtccgtt atgacgccgg gcgctccagc
     6181 ggcaaacgtc accggattgc ccgatccgcc gcccgtggcg gcaaggccga cgatggcaga
     6241 cgctttctgc ggcgcgacag gagagaaggt aattgtctgg ccggccttgc cgatcgtgag
     6301 cgtaccggtt tcctctcctt gccaattcgc gtcgttgacc gttccggtca cggcataact
     6361 cccggcctcg ctcggcgaag tggatgagcc gttgtaggta aactcgacgg tgagtccggc
     6421 gggcatcgtc gtggccgatg cgctcttggc cgtgccgtcg taggtctggg tcagatccag
     6481 caggtacacg ttcgcagcgc ccttcgacac gaccagcgtt ccatccgcgg tgccttgcca
     6541 gttggcgtca tttactgtac cggtcaccgc ataactgcct gtgttggtcg gtgcccatgc
     6601 attgccgttg taggtgaact cgatggtcag gccggaagga tcactcgtcg ccgccacgga
     6661 ccgtgccgtg ccgtcgtacg tctgcgcgag gtccagcaga tacacttccg ccgcgccctt
     6721 cgagaccacc agcgtgccat ccgcggaacc ttgccagttg gcgtcattta ctgtaccggt
     6781 caccgcataa ctgcctgcgt tggtcggtgc ccaggcgttg ccgttgtagc tgaactcgat
     6841 ggtcaggccg gacggatcgc ttgtcgcggt cacactcttc gccaagccgt cgtaggtctg
     6901 cgcgaggttc agcaggtaca cgcctgcggt gcccttcgag accaccagtg tgccatcggc
     6961 ggaaccgacc caattaacat cattgaccgt gccggttacc gcatagctgc ccgcgttcgt
     7021 cggtgcccag gcattgccgt tgtaagtgaa ctcgatggtc aggcccgccg gatcactcgt
     7081 cgcggtcacg ctcttcgccg tcccgtcgta tgtctgcgcg aggtccagca gatacacgct
     7141 tgccgcgcct ttcgatatca ccagcgttcc cgtctgtgag ccctgataca tcacatcgtt
     7201 caccacgccc gtgatcgcgt agctgcccgc gttggttggc gcccaggcgt tgccgttgta
     7261 ggtgaactcg atggtcaggc cggaaggatc gcttgttgcc gtcacggacc gtgccgtgcc
     7321 gtcgtacatc tgcgtgaggt ccagcaggta caggcctgct gtgcccttcg agaccaccag
     7381 cgttccatcc gcggtgcctt gccagttggc gtcatttact gtaccggtca ccgcataact
     7441 gcctgtgttg gtcggtgccc atgcattgcc gttgtaggtg aactcgatgg tcaggccgga
     7501 aggatcactc gtcgccgcca cggaccgtgc cgtgccgtcg tacgtctgcg cgaggtccag
     7561 cagatacact tccgccgcgc ccttcgagac caccagcgtg ccatccgcgg aaccttgcca
     7621 gttggcgtca tttactgtac cggtcaccgc ataactgcct gcgttggtcg gtgcccaggc
     7681 gttgccgttg tagctgaact cgatggtcag gccggacgga tcgcttgtcg cggtcacact
     7741 cttcgccaag ccgtcgtagg tctgcgcgag gttcagcagg tacacgcctg cggtgccctt
     7801 cgagaccacc agtgtgccat cggcggaacc gacccaatta acatcattga ccgtgccggt
     7861 taccgcatag ctgcccgcgt tcgtcggtgc ccaggcattg ccgttgtaag tgaactcgat
     7921 ggtcaggccc gccggatcac tcgtcgcggt cacgctcttc gccgtcccgt cgtatgtctg
     7981 cgcgaggtcc agcagataca cgcttgccgc gcctttcgat atcaccagcg ttcccgtctg
     8041 tgagccctga tacatcacat cgttcaccac gcccgtgatc gcgtagctgc ccgcgttggt
     8101 tggcgcccag gcgttgccgt tgtaggtgaa ctcgatggtc aggccggaag gatcgcttgt
     8161 tgccgtcacg gaccgtgccg tgccgtcata cgtctgcgcc aggtcttgca gataaacgcc
     8221 tgccgctgcc ttcgtcacgt tgaaggtgtt tgtgacgttc ggggccggat tccagttcgc
     8281 gtcaccgatc tgcgaggcga cgatcaaaac gctgcccgtg ccggtgaaag agagattggt
     8341 gtcgcccgta atgagggccg aaccaccacc cacggagaat tgcacagcca aaccactcga
     8401 tgccgttgcc gccaagccca caagatttgt ggtcaactga tcaggaatcg ccgggaacgt
     8461 gatcgtctga ttctgcttaa cgccgtgccc cgaaaggttg agcaggtacg gcgtattcgt
     8521 gctgctgttc acgatcgaga ccgtgcaggt ggaggtccct gcggacacag gcgtgaagat
     8581 gacggagaag ttggagatcg tcccggccgc caccgtgacc ggcatgccgg cgatggcgaa
     8641 ggcgcccgcg ccggtcccgt tggtgggcca gctgctgatc gtcaggacgg cgtcgcctgc
     8701 attcgtgatc gcgaagcggt tggtcgccgc gtcccccaaa ttgatcgtcc caaagtccgt
     8761 tcctttagcc tcgctcaccg cctcgccgct ggccacggcc gcgccgttgg cgcccagcaa
     8821 cgcaaacagg ggcccggtgt aggtgaaggc gtccgacttg accgtctcgc catagctcgc
     8881 tgaatacacg cgcacatcac ccatgccggg atcgccggcg gcggcgacca cgaccacctg
     8941 tgtggcagac tggctgacga ttgacgccac gcttacgccg cacagggtca cgttggtgat
     9001 gtcgccgctg ccaagattac ttcccgaaat gaccacttca aaaccgcccg tgatcggccc
     9061 ggtgtccggg tccacacccg ttgaagtcac acctcccgct ccatagctgg cgatgtgcca
     9121 ggtgttcgtg tccgtcccat cgtcctgcgt ggcgatcctg aaatatccgc cagcatacac
     9181 atcgttgttg ttcgtattga cggttaatga aagcaccgtg tcgctgaggc cacgacccat
     9241 gtttgtccag tgcgtcccgt tccacatggc aatgcggcgg acggcgagtt gcggggcgga
     9301 cttgtccggt tcggtcgaga tgtaggtgtt cgtaaatgac ccgcccgcgt agagcgtacc
     9361 gtccggggcc accttgagcg catagaccca gttgttgaac cccgatccga cgttggttaa
     9421 ggattgcgaa accgggtccc atttcgcgac atagtttgcg ggatgcgggt ctcccgcgct
     9481 gtcgaaatag ttcgtgaacc agccgccgat gtacagctct ccgtttgcgg gattcacggc
     9541 catgcaggtc acccggttgc cgaaaccctg cgccatgttc gtccagttca caccatccca
     9601 ccgggcgatg tatctgatgt ggcgcgaggt gttcgtcgga taatgattag tgaacgagcc
     9661 tccgacgtac agatggtccc ggtcgcggtc atacgccgcc gccagtggca cgttgcgaaa
     9721 gcctccctgc atgttcgtcc agccggcgcc gtcgtatttg gcgatgtaat tcaggcccgc
     9781 cgcccgataa tcgctattgg tgaagtagcc gacggcgatc acggtgtcat ccttgtaggg
     9841 ttccatccag ttcacatagc cattgatgct gacggtaaaa tacaatcccg tcttgtttcc
     9901 gagcagcggc gcgtgtccca ttgcattcca actcgtgccg tcaaaacggg cggcggcgta
     9961 tgcgttgacg ccgccatcgt tgttcatcgt cccgtccggg ttatacgatc cgccgatatt
    10021 cgtaaaatag ccgccggaat aaatgccatg gggagatccc ttgatgcagt tcacgttggc
    10081 gaccctgagc acaccggcct gcatcgaatg ccagttcgtc ccgtcgtggc gcgcgacgcg
    10141 gtagcagttc gatccgccgg cattggtgaa cgcgccgccg atatagaggt tgttgccgta
    10201 gtaatccatg ccgtagatcg tgttgttcgc cccgacggcg gacacctgcc ccggcacaag
    10261 cgcctggccg accgattgcc acatccggga accggttccg ccggagatat ctcccgccgg
    10321 attgacggtg taagccgccg ccagcgtctt gtctccattg tccgaggtct ggatgacaag
    10381 gtccttcgcg cccgccgagc cggccgccgg cacggtgatc cgcacccagt tcgcgccgga
    10441 ggcttcgatc gtcgaggcca cgcccccgac cagcacgttg gtgatggttc caaagttccc
    10501 gttggtaatc gtgattgtgt tgccgccggc gaaaggacca ctgtacgcag aaagcgcgta
    10561 cacatggaag gtgttcgtca cattcggcgc cgggttgtag gtcgcgtcac ccgactgcga
    10621 gaccacaatg gacaccgtcc ccacgcccgt gaagctcagg ttggtccctc ccgtgattga
    10681 ggccgggccg cttcccaccg cgaagcccac cggcagcccg ctcgacgcag ttgccgccag
    10741 ccccacgctt cccgaaactt tttgcggcgg gacgggcaca aaggtgatga gttggtccag
    10801 ctttacgctc gggtccaccc aggcaaagac gtttgtgacc ccgttcgcca tgtccgtcga
    10861 tcccccggtg tggtagatct tgtttcccaa ggtcgcggcc gcagccagat accctttcgc
    10921 aagcggcaac cccgggctct ccgtccagtt ggttccatcg tagcagtaga cgttgctgtg
    10981 aacggtgccg tagacccccc ccatggcgta gagccggccc cccatctcgg ccgtcgaatg
    11041 atggctcctt ggcacgggca gcccggcgac agccgtccag ttcgttccat cgaatcggta
    11101 gacgttcgtg taggacgggg tcacgccgtc gtaacccccg acggcataga gccggccgtt
    11161 gtgaaccgag gccgacaggg ccatgttgac caccggcaag ccgaccgtct ccgaccaatt
    11221 ggtcccgtca aaacaataga cgttggtctg ggcgctgaca ccaaacctcc cgccgattgc
    11281 gtaaatcttc gactggaaag tgcatgcagc cagatactga cgggccgcgg gcagcgacgg
    11341 cgccaccgtc cagttggtgc cgttgaagct gtagacattc gttcgctcgc tgctgccatt
    11401 gtgtcctccg atcaggtaaa gcgtgccgtt cagcgtggcc gcgcccgccg aaatcaagcc
    11461 tccaaagcct cccaccggcg cccacggaat ggatgtccaa ttggttccgt cgagacggaa
    11521 ggcattagtc gaactgtagc tgtttggaat cgtacctccg cccagtacat agagtttatc
    11581 gttcaggaca gccgcgcagt ggttcaccgt ttgcatcgga agcccgatga cgttggtcca
    11641 cgaacccgca gcctcagcaa ccccgcctgg cgcactcagc cacatggcta tggccacaag
    11701 tgcggccacg aaagagaaac gcagacgggt gaatgggaaa cggcgactga gcatggcaac
    11761 atctctgaca tgacgcaccc tgcggcgaaa ccagggcata acaagacacg aaccgcacga
    11821 cgtacggcgg gcagttgaaa aagagaacgg agccctgcgc tcagcataaa acccgctttg
    11881 aattccagtt ttcatggtaa cctcctaagt attgacaggc aaacggcatg gaaaaagttc
    11941 caatcagtga aactccccgc ggcaagccgc ggggcatctt tcagcg
//
LOCUS       DAOQWR010000077        11941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5843_length_11941_cov_4.560407, whole genome shotgun sequence.
ACCESSION   DAOQWR010000077 DAOQWR010000000
VERSION     DAOQWR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5843_length_11941_cov_4.560407"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1630)
                     /locus_tag="PKK36_07300"
     CDS             complement(221..1630)
                     /locus_tag="PKK36_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94398.1"
                     /translation="MATTPAQIDELLQARSEHEHLEFKAAENSYEFEELVDYCAALAN
                     ERGGRMILGVTDKIPRRVVGTKAFAVPARTVAGIHDRLHIKVTCDEVIHPGGRVLVFN
                     VPSRPLGQPIQYRGRYLMRAGEQLVPMTPDQLKAIMLEGSPDWSLMPALTGCDGETIV
                     QLLDTQSYFDLRELPYPGTREAVLERFAGERLIEKSGSGWTVTNLGAILFAKKLDQFD
                     LLARKAPRVIVYEGSNKLKTKLDRQGLKGYAVGFQGLVEFINSLVPSNEVIEKALRRE
                     VKMFPEIAVRELVANALIHQDFNESGTSVMIEIYSDRMEISNPGKPFIPPDRFIDEYQ
                     SRNERLADIMRRLGICEEKGSGIDKVIEAAEVYQLPAPDFRVGEKHTSVVLFAHQDIE
                     EMDRNDRIRACYQHCCLRYVMNQSMTNQSLRDRFKLAETKTATVSQIISATVDAGKIK
                     AADPTQTSTRYRSYVPFWA"
     gene            complement(1931..3085)
                     /locus_tag="PKK36_07305"
     CDS             complement(1931..3085)
                     /locus_tag="PKK36_07305"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR94399.1"
                     /translation="MKKGWETKNLGDLCDILDHKRKPITKRDRIAGDYPYYGATGILD
                     HVAGYLFDEPLVLLGEDGAKWESGENTAFPVSGKIWVNNHAHVLRPHRMELLDNWLIH
                     YLNHSDLNEFVSGLTVPKLNQGNMREIPIPLPPLPEQKRIVGILDEAFAGIATAKANA
                     EKNLQNARALFESHLNDVFTKRGKGWVEKRLDDVCVIGDGNHSSNYPKKEELVAAGVP
                     FIRATNLVGGQISDDDMRYLSPEKHAQLKKGHLKTGDVLFTNRGEIGKTAIVDRAHDG
                     SNLNSQIAWFRCRNELCNRFLFYVLNSGKMKMHINLSQNGAALQQFTIRQIKELQIPV
                     PPQTEQLTVVKKIDQLSKEVDHLEFIYQHKLDELDALKKSLLHQAFTGAL"
     gene            complement(3082..4533)
                     /locus_tag="PKK36_07310"
     CDS             complement(3082..4533)
                     /locus_tag="PKK36_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR94400.1"
                     /translation="MFEQAFKNIDDVLWKEAGCTTELDYTEQTSWLLFLKYLDGLEQD
                     KATEAALEGKKYSFILEKPYRWETWAAPKGKDGKLDHNSARTGDDLRDFVNQKLFPYL
                     HGFKQRASGPNTIEYKIGEIFGEIKNKIQSGYNLREIIDHIDELRFRSQAEKHELSAL
                     YEEKIKRMGNAGRNGGEYYTPRPLIRAMVKIVKPKIGETIYDGACGSAGFLCEAFDYL
                     KTGNLTTKELKKLQEKTFYGREKKSLAYVIAIMNLILHGIEAPNIIHTNTLSENLADI
                     QEKDRYDIILANPPFGGKERKEVQQNFPIRTGETAFLFLQHFIKILKAGGRAGVVIKN
                     TFLSNTDNASVSLRKLLLESCNLHTVLDCPGGTFLGAGVKTVVLFFEKGTKTRKTWYY
                     QLDPGRNMGKTNPLNDDDLAEFVKLQKNSADSEKSWTVDAKTIDQTNFDLSVKNPNGG
                     EEITHRSPKEILKEIAALDAESAKVMEKIKALI"
     gene            complement(5021..5197)
                     /locus_tag="PKK36_07315"
     CDS             complement(5021..5197)
                     /locus_tag="PKK36_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94401.1"
                     /translation="MKNLVRPLTLGLVLLLICSPAVRARLKDSETEARERYGQIAARD
                     AESAKVIEKIKALV"
     gene            complement(5407..5790)
                     /locus_tag="PKK36_07320"
     CDS             complement(5407..5790)
                     /locus_tag="PKK36_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HNR94402.1"
                     /translation="MSHKKPDPGKLARLHADLARERRERETSYRGRALAMFPHICASC
                     GREFEGARLRELTVHHKDHDHTHNPPDGSNWELLCLYCHDHEHEKYLMKGHGGGSRPA
                     ETSAPIANPFENLDAKLKLDGTEKQ"
     gene            6666..7304
                     /locus_tag="PKK36_07325"
     CDS             6666..7304
                     /locus_tag="PKK36_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94403.1"
                     /translation="MRLWRAILILGLACAAPASEPGRGPPLNITTGTIHRVGTVAALQ
                     NAVAAVNAASAPATILISNGVYVLTDWALPITNNNVIIRSLSGDRDAVVIRGPDEGES
                     AALQHVFWVTASNFTVADITFGWCRYHGIQAHGQEPHNVSGLWVHNCRIVNCNEQFIK
                     GTSADGDPEGVTDSLVEYCLFEFTNQWAYQYYTGGIDAHKAVNWTVRDNRFE"
     gene            7521..8291
                     /locus_tag="PKK36_07330"
     CDS             7521..8291
                     /locus_tag="PKK36_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94404.1"
                     /translation="MGIGLEYANNVSVDNNTVYVPYWAPIEYRFAGSSNLVFRNNLVD
                     GPITARDGAPAAAQTGNIETVQAQWFRSPTSGDLRLLPGATSVIDQGATLVDFSDDIY
                     GESRPVGSAWDVGADEYDPHRSDSDGDTIPDYWEIAYSLNPTSAADAAENADGDFFSN
                     LQEWTADTDPGNSNDYFQITGVSNAYPIVLTCHGASNRLYTLLYKTNLAEDTWQEHPG
                     QTTVASDGSLTVLTDTNMPAERMFYRIMVTIDDAAFSF"
     gene            complement(8294..9265)
                     /locus_tag="PKK36_07335"
     CDS             complement(8294..9265)
                     /locus_tag="PKK36_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNR94405.1"
                     /translation="MENSCRKAVGVDFGGTGVKMAVVDDQGTILSRGGFATADASSQK
                     DWLHAVEQELHKITAAAGLTLNDLDGVGVGVPGFVDFARGYVHNLANVPGWTGVYLKE
                     MLRQQLRLPAVIDNDVNAMALGESKFGAGRNYDHVVFVTLGTGVGGGILIHGKIYRGA
                     YSTAGEIGHMSIDMNGIKSPEGSGGLEQYVGNRRIVERAVRLIGEGRPTGILELAGGR
                     TDNITPKIIAEAAHAGDPLALEIFDFVADCLAAAFASITYLIQPEVFILGGGVAQSGR
                     VLFDPLMRHLTGRLSPFIAEHVSVIPAELGSDAGVIGAAGLLWTPEA"
     gene            complement(9268..10044)
                     /locus_tag="PKK36_07340"
     CDS             complement(9268..10044)
                     /locus_tag="PKK36_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR94406.1"
                     /translation="MFTDTHVHFDVFNENGGLDPVIARAREAGVRRMLAIGGSAAANL
                     LALRIAREHPDELRAAVGYDREVCLSGPDISALKALVDDPFAVAVGETGLDYHYHPET
                     GNAQRDLFARMLEMAGEHGLPAVIHSREAEEDTLDLLDRNPGLKGVLHCFTGTVEFAR
                     ELLKRGICISFSGIVTFGNAAGPAAAAALVPEDMILAETDAPYLAPVPHRGKRNEPAF
                     VVETVRKLAQIRNCSVEQMAAITSRNAARLFGWRVSESGE"
     gene            complement(10059..11615)
                     /locus_tag="PKK36_07345"
     CDS             complement(10059..11615)
                     /locus_tag="PKK36_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNR94407.1"
                     /translation="MALALSCALPASRAAASGPEQTNVIVIANQNVPASLEAARYYLA
                     ARGLPEASLCVLDLPSGELISRSIYDLKLREPLLEFLRKNNFIEQKQRNPNEVQPHAS
                     KWLTLRSSVQCLVPVYGVPVKIADTKFPLAVKISSALGKGLYRDSAAVDSELALLLAA
                     PYVIEGPHNNPLYDQYALIPSPSGEGAPLLVSRLDGPDIESVKNMIDGALFAERYGLC
                     GRMYFDLQETSDPLYYAGDHWIREASERFAREGYECHLDRTANVFPPEYPMTDATLYF
                     GWYTEHVTEPFTRKDFRFLPGAVAYHIHSASAKMLRGRTRYWTGPLLAAGAAASMGAV
                     GEPYLGMSPNLDIFSDRLCSGSSMGAAALFSLRALSWQTTVTGDPLYAPFRYPLDVQI
                     RHLQEDGRPEIEWAWLRKVNLMVRAGALNPALEFCREKIAETGSLVLREKLADLYVKN
                     ALYNDAVDQYRAVLAAAADAGTAMRVGARLLLLLRLQNRADAADELENELKQKWKDSP
                     FLPWLDTARP"
BASE COUNT         2527 a         3351 c         3258 g         2805 t
ORIGIN      
        1 cccagcccct cgatccgtcc gggcgcaatg tgatattccc gcaggatccg gctgccctca
       61 accacacccc atcccgccgc cttcagcgcg ggatcaatat gctcggctct ggtctcggct
      121 tcgttcattt tagggttagc gtttatacaa gataaattct aaatatcaac gacttatgca
      181 catctgctcc aaaatatcgc ttttgctttt aagcagaaat ctaagcccaa aacggcacat
      241 aactgcgata ccgtgtagac gtttgcgtag gatccgccgc cttgatcttc ccggcatcca
      301 ctgttgcaga aataatctgt gaaaccgtag ccgttttagt ctcagccaac ttgaagcgat
      361 cccgcaaact ctgatttgtc atgctctggt tcatcacata tcgcaaacag cagtgctgat
      421 aacatgcacg aatacggtcg tttcggtcca tttcctcaat atcctgatgt gcaaataaga
      481 caaccgatgt atgcttttct ccaactcgaa aatcaggggc aggaagttga taaacttctg
      541 ctgcctcaat gaccttatca atcccactgc ccttttcttc gcagatcccc aaacggcgca
      601 tgatatcggc caagcgctca ttgcgtgact gatattcatc aataaaacgg tcggggggga
      661 taaatggttt cccgggattc gatatttcca ttcgatccga gtaaatttct atcatcacgg
      721 atgtcccaga ctcattgaaa tcctgatgaa tcagtgcatt tgcaaccaac tctcgaacag
      781 caatctctgg aaacatcttc acttcccgac gcagggcctt ctcaataacc tcgttggatg
      841 gaaccaggct gttaatgaac tcaaccaggc cctggaaccc gactgcgtat ccctttaagc
      901 cctgcctgtc caacttcgtc tttaacttat tggagccttc ataaacaatc acccgagggg
      961 ctttcctggc cagaagatca aactgatcga gtttcttcgc aaacaaaatc gcgccaagat
     1021 ttgtaaccgt ccatccggaa cccgacttct caataaggcg ttcacccgca aacctttcca
     1081 gcacggcctc acgcgtcccg ggatatggca gctcacgcaa atcaaaataa ctttgagtat
     1141 ccagcaactg cacgatggtc tcgccatcgc aacctgtcag cgccggcata agtgaccagt
     1201 ccggagaacc ttcaagcatg atggccttca attgatcagg cgtcatcggc accaactgct
     1261 cacctgcacg catcaaatat cggcccctgt attgaatcgg ttggccaaga ggacgggagg
     1321 gaacattaaa caccaatact cgcccaccag gatggatcac ctcatcgcag gtcactttaa
     1381 tatgcaaacg gtcatggata ccagccactg tacgggccgg aacggcaaac gcctttgttc
     1441 caaccacccg acgcggaatt ttatctgtga ctcccagaat cattcgcccg cctcgctcgt
     1501 tcgcaagagc tgcgcaataa tctaccagct cctcaaactc atagctattt tcagccgcct
     1561 tgaattcaag gtgctcgtgt tcggaacgag cctgaagcag ttcatctatt tgtgccggtg
     1621 ttgtcgccat agtcaactgt catcctgatc tcggcgttgt cttgcgtcaa tcctgttcat
     1681 gttcttccaa tccctggacc cgagccttgg gagagcggcc ctccatgtgg agggcaatgc
     1741 ccgcagggca tggcaacttt ataccagaaa agttccaacc attggaagtt ttggacggaa
     1801 aagttccagt cattggaact ttttggcgcc ggacttccag acattggatg ttttctgcac
     1861 gcattttcca gcgattggaa gtttttgggt gcgtttttcc aatcattgga ggtttgtgca
     1921 aggctggcca tcacagcgct cccgtgaagg cctgatgcag cagcgatttt ttcagcgcat
     1981 ccaattcatc cagcttgtgc tggtagatga attcaaggtg atctacttct ttagagagtt
     2041 gatcgatctt cttaacgaca gtcagctgct ctgtttgggg aggaactggt atttgaagtt
     2101 cctttatttg tcttatcgtg aactgctgca atgctgcccc attttgcgac aaattaatat
     2161 gcatcttcat tttgccagag tttagaacat aaaacaaaaa gcggttacat aattcattgc
     2221 gacatcgaaa ccatgcaatt tgcgagttga ggttcgagcc gtcatgggct ctatcaacga
     2281 ttgcggtctt gccaatctca cctcgatttg taaacagaac gtcacctgtt tttagatgcc
     2341 ccttcttaag ttgtgcatgt ttttccggcg atagatatct catatcgtcg tcagagattt
     2401 gcccccccac aaggtttgtt gcacggataa acgggactcc agctgcgact aattcttcct
     2461 tcttcggata attagaagaa tggtttccat caccaatgac acaaacatcg tctagccgct
     2521 tctccaccca ccccttgccc cgcttcgtga acacgtcatt caggtggctt tcgaagaggg
     2581 cgcgggcgtt ctggaggttt ttttcggcgt tggctttggc ggtggcgatc cccgcaaacg
     2641 cttcgtccag tatccccacg atccgctttt gttcggggag aggaggaagt gggattggaa
     2701 tctctcgcat atttccttgg ttcagcttcg gaaccgttag cccagagaca aactcattca
     2761 gatcagaatg gttaagataa tgaatgagcc agttgtccag aagttccatc ctatgtgggc
     2821 gcaaaacatg tgcatgattg ttgacccaaa ttttaccgga aaccggaaat gccgtattct
     2881 cgcccgattc ccacttcgcc ccatcttctc caagaagaac aagtggttcg tcgaaaagat
     2941 atccggcaac atggtcgaga atgcccgttg ctccgtagta gggataatcg cccgctatac
     3001 ggtctctttt ggtgatcggc tttcgtttat gatcaaggat atcgcaaagg tcaccgaggt
     3061 ttttggtctc ccaccccttt ttcatatcag cgccttgatt ttctccatga cttttgcgct
     3121 ttcggcatcg agggcggcga tttccttgag gatttctttc gggctgcggt gcgtgatttc
     3181 ctcgccgccg ttggggtttt tgacggagag gtcgaagttc gtctggtcta tggttttggc
     3241 atccacggtc cagctttttt ccgagtcggc ggagtttttc tggagtttga cgaattcggc
     3301 aaggtcgtcg tcgttgagcg ggttggtctt gcccatgttg cggccgggat caagctggta
     3361 gtaccacgtt ttccgggtct tggttccctt ttcgaagaac agcacgacgg ttttgacgcc
     3421 ggcccccagg aaggttccgc cggggcagtc cagcacggtg tgcaggttgc agctttccag
     3481 caacagtttc cgcagactca cggaggcgtt gtcggtgttg gacaggaagg tgtttttgat
     3541 gacgacaccg gcgcgtcccc cggctttcag gattttgatg aagtgctgga gaaacaggaa
     3601 cgccgtctca cccgtgcgga tcgggaagtt ctgctgcact tccttgcgct ctttgccgcc
     3661 aaacggagga ttggccagga tgatgtcata gcggtccttt tcctggatgt cggcgaggtt
     3721 ttcggagagc gtgttggtat ggatgatgtt tggggcctca atgccgtgca ggatcaggtt
     3781 catgatcgcg atgacgtagg ccagcgattt cttttccctg ccgtagaagg tcttttcctg
     3841 aagctttttg agctctttgg tggtgaggtt gccggtcttc aggtagtcga aggcttcgca
     3901 caggaatccg gccgatccgc aggcgccgtc atagatggtt tcgccgattt tgggtttgac
     3961 gattttgacc atggcgcgga tgagggggcg cggggtgtag tattccccgc cgttgcgccc
     4021 ggcattgccc atgcgcttga ttttttcctc gtagagggcg gagagttcgt gcttttccgc
     4081 ctgcgaccgg aagcgcagtt cgtctatatg gtctatgatt tcccgcaggt tgtagccgct
     4141 ctggatcttg tttttgattt caccgaagat ttcaccgatt ttgtattcga tggtgttcgg
     4201 cccgctggcc cgctgcttga agccgtggag atacgggaaa agcttctggt tcacgaagtc
     4261 ccgcaggtcg tccccggttc tggcgctgtt gtggtcgagt ttgccgtctt tgcccttggg
     4321 ggcggcccag gtttcccagc gatagggctt ttcgaggatg aaggagtatt tcttcccctc
     4381 cagcgcggct tcggtggcct tgtcctgctc caaaccgtcc agatatttca ggaaaagcag
     4441 ccaggaggtc tgttcggtgt agtcgagttc ggtggtgcat ccggcctctt tccagagcac
     4501 atcgtcaata ttcttgaagg cctgttcaaa catgggaccc gcttcctttc aaacaaccgc
     4561 tggcccgtat tgttggactt tggggaaagt cccgtcaaga gcgtagttat gcgccagata
     4621 cacctgagca aattaggatt ttggggttag gtcttaacat ttaacattcg tttcgttact
     4681 gtctgcgttt ctgagcggca atcttatgct tttcgcatca ttcgttttcg ctgctcacat
     4741 cctcatcttc ggcggccaag ggactggccg ccctaccatt caaaatctcc gtgcctctgt
     4801 gtctccgtgc gagaatcatt tcttttgcgc gcttttgtgt tccttgcggt gaaatatcag
     4861 ttcatcccct ccgcgtccct gcgcctctgc gcgcggcttt tcttttcaca tcattcgttc
     4921 tcccgctgtt tccccctagt cttccggtct cccgaggaat gggaatcagg tgatggagca
     4981 tccgccctgc ccgcatttct cacattattc ccattgttat tcataccagg gccttgattt
     5041 tctcgatgac tttggcgctt tcggcatcgc gggcggcgat ttgcccgtaa cgctcacgcg
     5101 cttcggtttc cgagtccttg aggcgggccc ggacggccgg gctgcagatg agaaggagaa
     5161 cgagaccgag ggtcaagggc cggaccagat ttttcatatt ttcgctttct cctgcacccc
     5221 ctcgccgttc cgaagaataa cgccggagcg gattggaaag ccagaagaaa gtgggggaaa
     5281 aaaagaaggg gtcaggggtc agggttcagg gttcgggagg cgggaagaag ggttcagggt
     5341 tcaggagaca ggagacagaa ttcaggagtc agaataatat tctgaatact gtcttctgac
     5401 tcctgattac tgtttttccg tgccatccag cttcagcttt gcgtcgaggt tttcgaacgg
     5461 gttggcaatc ggtgcggatg tttcggccgg gcggcttccc ccgccgtgac ctttcattag
     5521 atatttctca tgctcgtggt catggcagta caggcagagc agttcccagt tgcttccgtc
     5581 cgggggattg tgggtgtgat cgtgatcctt gtgatgcacg gtcagttcgc gaagccgggc
     5641 cccttcaaat tcccgtccgc agctcgcgca gatgtgcggg aacatggcca gcgcgcggcc
     5701 gcggtaactc gtttccctct cccgccgttc gcgggcgaga tcggcgtgaa gccgcgccag
     5761 tttcccggga tcaggttttt tatgactcag agacaaaatc tctccttatc tgaactcaaa
     5821 cgccggaagt catagtaaca cggtttacgg aaaatttgtc cacaaaaggc acagaaggga
     5881 aagaagaaga attcagaagt cagaagtcag gaggcaggag gcgggaagaa ggggtcaggg
     5941 ttcagggttc agggttcagg gttcaggagg cgggagacag gagacagaat tcagaagtca
     6001 ggattcagaa gtcagaatgc ggctttttag actggaagcg gttatatagt actaaattag
     6061 tactatttcg atattttggg tgttttttgc gaattatggg ccatttttag cgttttttgg
     6121 ccattttggg gcatttttga tcatttttgg gttctactac gattttcgtg gaaacattgg
     6181 gcgaaatgga tgcggcagca gacaggaaac gggaagcagg actcagtcag atctgggaac
     6241 atgaaactga acttcggggg aatcggcgtg atcacagcgg cggtgtagta gcgtctctat
     6301 gagacgcatt ctgaaaacat gtgccgctca cagagcagct actacatcag aaaccctgca
     6361 aataaaggca tttgcgcatg tatcgatcat ggaccgatgc ttaccagcca ttagaagccg
     6421 tatgtgaatt cgtctgaaag cggcatcaag ctgccgcact ccagagcgcc gcgaaggcgg
     6481 cgcagcattt ttcatgaaac gatcagaagc tggttttgag cctgaaatcc tccacgataa
     6541 ttgacgaaga acccattttt ggccgttttt tacgtttttt gagcattttg gggcattttc
     6601 acgctggtcg cagtttctgc gcagtatcat tcccaaatgg catgttttat gctcatactg
     6661 catgcatgag gttgtggaga gccattttaa tactcggact tgcctgcgcc gccccggcat
     6721 ccgagccggg ccgcgggcct ccgctcaaca tcaccaccgg gaccatccac cgcgtcggga
     6781 ccgtggccgc gcttcagaat gccgtggcgg ccgtgaatgc cgcaagcgca cccgccacca
     6841 tcctgatttc caacggggtc tatgtcctga ccgactgggc cctgcccatc acgaacaaca
     6901 acgtcatcat ccgcagcctg agcggcgacc gcgatgcggt ggtcattcgc ggcccggatg
     6961 agggggagtc ggccgcactc cagcatgttt tctgggtgac cgccagcaat ttcacggtgg
     7021 cggatattac attcggctgg tgccgctatc acggcattca ggcgcatggg caggaacccc
     7081 acaatgtttc cggcctttgg gttcacaact gccgcattgt caactgcaac gagcagttca
     7141 tcaagggaac ttcagcagac ggcgatcccg aaggcgtgac ggacagcctt gtggaatact
     7201 gcctttttga attcaccaac cagtgggcct atcagtatta cacgggcgga atagacgctc
     7261 acaaggcggt caactggacc gtgcgcgaca atcggtttga gtaaatccgc gcccccgaca
     7321 gctatgccga gcacgccatc cacttctgga accgcaacac cagccgcgcg cagaacatca
     7381 ttgtcgagcg caacatcatc atcaactgtg accgcgggat cggttttgga ctttcaagct
     7441 acggcggggg ctttgaaggc ggcgccagcg ccatccgcaa caattttatc tataacaacg
     7501 gggacgggcc ttataccgat gtgggaatcg ggctggaata cgcaaacaac gtcagcgtgg
     7561 acaacaatac agtctatgtc ccctactggg ccccgatcga ataccgcttt gccggcagtt
     7621 ccaatctggt gttccgcaat aatctggtgg acggccccat cacagcgcgc gacggagccc
     7681 cggccgccgc gcaaaccgga aacattgaaa cagtgcaggc acagtggttc cgcagcccga
     7741 ccagcggcga tctccgcctg ctgcccggcg cgacctctgt tattgatcag ggcgcaaccc
     7801 tcgttgattt ctccgacgac atctacggcg agagccgtcc cgtcggctcc gcatgggatg
     7861 tcggcgccga tgaatatgat ccccaccgtt ccgattccga cggggacacc atcccggact
     7921 actgggaaat cgcctatagt ctgaatccca ccagcgcggc agatgccgca gagaatgcag
     7981 atggagattt tttcagcaat ctgcaggaat ggacggccga caccgacccg ggaaacagca
     8041 acgactattt tcagattaca ggcgtatcaa acgcataccc catcgttctc acctgtcacg
     8101 gcgcatccaa ccggctctat acccttctgt acaaaaccaa tctcgctgaa gacacatggc
     8161 aggaacatcc cggacagacc accgtcgctt cggacggcag tctgaccgtg ctgaccgata
     8221 ccaacatgcc ggccgaaagg atgttttacc ggattatggt aacaattgat gacgccgcgt
     8281 tttcgttctg acattacgct tccggggtcc agagaaggcc cgccgcgcca atgacgcctg
     8341 catcactgcc cagttccgcc ggaatcacgc tgacatgttc ggcgatgaac gggctgaggc
     8401 ggccggtcag atgccgcatc agcggatcga agagaacccg gccgctctgc gcgacgccgc
     8461 cgccgaggat gaaaacttcc ggctggatca gataggtgat ggatgcaaag gccgccgcaa
     8521 gacagtcggc cacgaaatca aagatctcca gtgcgagcgg atcgccggca tgggcggctt
     8581 ccgcgatgat cttcggcgtg atgttatccg tgcggccgcc ggccagttcc aggatgccgg
     8641 tcgggcggcc ctcgccgatc aggcgcacgg cgcgctcgac gatccggcgg ttccctacgt
     8701 actgctccag gccgccgctg ccctcgggac ttttgatgcc gttcatgtct atggacatgt
     8761 gcccgatttc gcccgcggtg gaataggccc cgcgatagat ttttccgtgg ataagaatgc
     8821 cgccgcccac cccggtgcca agggtgacga acaccacatg atcatagttt cttccggctc
     8881 cgaattttga ttctcccagg gccatggcat tcacatcgtt gtcaatcacg gccgggagcc
     8941 tgagctgctg ccggagcatt tccttgagat agacgcctgt ccatccgggc acgttggcca
     9001 ggttatgaac gtagccccgc gcgaaatcca cgaagccggg gaccccgacc cccaccccgt
     9061 ccagatcgtt gagcgtcagg ccggcggcgg cggtgatttt gtggagttcc tgctcgacgg
     9121 catgcagcca gtccttctgc gacgaggcat cggccgtggc aaacccgccg cgcgaaagga
     9181 tggttccctg atcatccacg acggccatct tgacccctgt tccgccgaaa tccacgccca
     9241 ccgccttgcg acacgagttt tccatgttca ttctccgctt tctgaaaccc gccagccaaa
     9301 caggcgggcg gcattacggc ttgttatagc ggccatctgt tcaacactgc aattccgtat
     9361 ttgggcaagt ttacgcacgg tttccacgac gaaggccggt tcattccgct ttccccggtg
     9421 cgggaccggc gccagatacg gggcatccgt ttccgcgagg atcatatcct ccggcacgag
     9481 cgccgcggcc gccgccggcc cggcggcatt cccgaaggtg acaatgccgc tgaagctgat
     9541 gcagatgccg cgcttcagaa gttcgcgggc aaactcgacg gtgcccgtga agcagtgcag
     9601 cacccccttc aatcccgggt tccggtccag caggtccagt gtgtcctcct ccgcctcccg
     9661 gctgtggatc accgccggga gcccgtgctc gcccgccatt tcaagcatgc gcgcaaataa
     9721 atcccgctgc gcatttccgg tttccggatg atagtgataa tcaaggcccg tttctccgac
     9781 ggcgacggca aacgggtcgt ccaccagtgc cttcagcgca gagatatccg gcccggacag
     9841 gcacacttcg cggtcgtatc caacggccgc gcgcagttca tccggatgct cccgggcgat
     9901 gcgcagggcg agcagattgg cggcggccga accgccaatg gccagcattc tccgcacgcc
     9961 ggcctcacgg gcccgggcga tcacgggatc gagcccgccg ttttcattga acacatcgaa
    10021 atgaacatga gtgtcggtga acatgacggc atttgtcctc aaggccgggc cgtatcaagc
    10081 cacggcagaa acgggctgtc cttccatttc tgtttcagtt cattctccag ctcatcggcc
    10141 gcgtccgccc ggttctggag gcgcaggagg agcagcagcc gggcgcccac gcgcatggcg
    10201 gtgccggcgt cagccgcggc cgccagcacg gcgcgatact gatcaacggc gtcattatac
    10261 agcgcattct tgacatacag atccgccagc ttctcccgga gcacgaggct gccggtttcg
    10321 gcgatctttt cccggcagaa ttcgagcgcg gggttgagcg caccggcgcg caccatcaga
    10381 ttgacctttc gcagccaggc ccattcgatc tccgggcggc cgtcttcctg aagatgcctg
    10441 atctgcacgt cgagcggata gcggaaaggc gcatacagcg gatcaccggt cacggtggtc
    10501 tgccatgaaa gcgcgcggag cgaaaagagc gcggcggccc ccatgctgct gccggagcac
    10561 aaccggtcgc tgaagatgtc caggttcggg ctcatgccca gatagggttc gcccaccgcc
    10621 cccatggatg cagccgctcc cgctgcaagc aggggtcccg tccagtaccg cgtgcggccg
    10681 cgaagcattt tcgcgctggc gctgtggata tgataggcca ccgcaccggg gagaaatctg
    10741 aaatctttgc gggtgaaggg ttcggtcaca tgttcggtgt accagccgaa atagagcgtg
    10801 gcatccgtca tgggatattc gggagggaac acattggccg tacggtcgag atggcattcg
    10861 tacccctcgc gggcgaatct ttcgctggcc tcgcgaatcc agtggtcgcc cgcataatac
    10921 agcggatcgc tggtctcctg cagatcaaaa tacatcctgc cgcacaggcc gtaccgctcg
    10981 gcaaaaagcg cgccgtctat catgttcttc actgattcaa tatccggtcc gtccaggcgc
    11041 gacaccagca ggggggcccc ctcgccggaa ggagaaggga tcagggcata ctggtcataa
    11101 agagggttgt tgtgcgggcc ttcaatgaca tacggagccg ccagcagcag ggccagctca
    11161 ctgtccacgg ccgcagagtc ccggtaaagg cctttcccca gcgccgaaga aattttcacc
    11221 gcaaggggaa acttcgtgtc cgcgattttc accgggaccc cgtacacggg gaccaggcac
    11281 tgaacagaag acctgagcgt gagccacttt gaagcatgcg gctgaacctc gttcgggttg
    11341 cgctgcttct gctcgatgaa attgttcttc cggaggaact ccagcagcgg ctcgcgcagc
    11401 ttcaggtcat agatgctgcg ggaaatcagc tcgccggacg gcaggtcaag cacgcagagg
    11461 ctggcctcgg ggaggccgcg ggccgccaga taatagcgcg ccgcctcaag tgacgccggc
    11521 acgttctgat tggcaatcac aatgacattt gtctgctccg ggccggatgc cgccgcacgg
    11581 gaggccggca gggcacagct caatgccagc gccaaaatgc acagcccttg cctgaaacaa
    11641 ctcatggagg cgattttgaa caattgcctg aaataatcca taaaaatcgg ttcaagcctc
    11701 attgcagaca taccggatcc atattagtct gttttatatt tgagttaatt taaccggata
    11761 atgtataaag ggtatggtta gggaatagcc gatcgaggag agacagcaaa tgaagcaaac
    11821 aattgttctg ctgatggccg gcatgctctt aagcgccagt gcgtcagacc tccgtattga
    11881 tggcggcaaa cttaacctga tcgggaaaac cgaggggggg gatgtgacgc ttggagcggg
    11941 g
//
LOCUS       DAOQWR010000078        11752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5934_length_11752_cov_5.041891, whole genome shotgun sequence.
ACCESSION   DAOQWR010000078 DAOQWR010000000
VERSION     DAOQWR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_11752_cov_5.041891"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKK36_07350"
     CDS             complement(<1..189)
                     /locus_tag="PKK36_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94408.1"
                     /translation="MAGGRRCSSDVFFPADFFLMRRGTGPALEDGMNEADMIFVEDRS
                     ILIYSSTLLLGRDLGSGLW"
     gene            447..1970
                     /gene="malQ"
                     /locus_tag="PKK36_07355"
     CDS             447..1970
                     /gene="malQ"
                     /locus_tag="PKK36_07355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNR94409.1"
                     /translation="MLSERKSGILLHPTSLPGNWGIGEIGPEACRFADTLHEMGQHLW
                     QILPMGPTSYGDSPYQALSTFAGNHLLISFDLLVQDGLLSESRLARFPKFDPSKIDYG
                     PVIIERMKVLQTVCRSFERCATDRMKREFQQFCRRQARWLDDYALYMAIKDSQNGKPW
                     IKWPRALAGRKPEAMNEAVKKFKTSIRNVKILQFIFHDQWRRLKTYCHRKNIKIIGDI
                     PIFVAHDSADVWAHPDLYYLDGRGNLTVQAGVPPDYFSATGQLWGNPLYRWDVHAQSN
                     YAWWAARLQHIFQMVDVVRIDHFRGFEAYWEVAGDAETAISGQWVKGPNHGLFDSLLQ
                     QLGPLPIIAEDLGVITPEVEKLRDDFGFPGMRILQFAFGNDPQAENFRPHNFPASCVV
                     YTGTHDNDTTVGWFHSKPDESSTRTQQDIDEERRRILDYVHTDGSQIHWDMISLAAHS
                     RANTAVVPLQDVLGLDSSARMNRPGIAGGNWGWRFHWEQLTPAIIRRMRDITASGKR"
     gene            2317..3618
                     /locus_tag="PKK36_07360"
     CDS             2317..3618
                     /locus_tag="PKK36_07360"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNR94410.1"
                     /translation="MKTYGVGILGFGTVGAGVIETLQLNGDLIAARTGLRIEVRRVAD
                     LDLDRDRGVKVDPSIFTRDAMAVIDDPAVDIVVELIGGVKTAREVILKALRAGKPVVT
                     ANKALLAEHGDELYAVADESSADLYYEASVCGGIPVIRSLREGLIANHIESMYGIMNG
                     TCNYILTRMEQENLPFEKVLADAQTRGYAEAEPSLDIDGIDTAHKAAILASLAYGFPV
                     PMEAVHVEGIRGLDPLDIRYAKELGYRIKLLAQIRHHEDGIEVRVCPTLVPLGHMLAS
                     VSGTFNALMVRGDVVGDTLYYGRGAGRLPTASAVVSDLADVARNLASSSNLRVPALVR
                     HGEYGKMKKLEDLESCCYLRMSLKDHPCVLAQVAQVLGENGISIASVIQNKPGEGCDY
                     APVIVVTHRAPEKKFRQALRIIDALDVVGARTIRMAIEDFE"
     gene            3624..4808
                     /locus_tag="PKK36_07365"
     CDS             3624..4808
                     /locus_tag="PKK36_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94411.1"
                     /translation="MKKIIIVCDSVADAPLEELDGRTPLQTARCPFASRLAQTGQTGL
                     LTLPRAGTEDHGEQLLGLFAGMTREQASALRQGPLEAMASGIDLSDYDFAWCGHFVTM
                     DGDVLRDSLVRSLSLEETRWLAEQLQREWDAQRVRFAATEPSRLLVLYSTAGGARPQP
                     GHPPHLSEGARADEFIRRSRADNIVRDIFERSRRILDSQPLNEIRVDLGENPANALWL
                     WGGGPMSGMHELFTGFRISGAMLTQCPMAAGLARLAGMETIEMRDVWTAGGGMAFNLP
                     LLVQELRQKDLLLVYAGAPRPGGRYGSAVEKVRAIEAMDANLLGPLSDVLAAHRPCRI
                     ILAANRGVSTPTGLPEAGRIPFVLAGDGIGADETQNWNEEEAARGALGSVKLSRLADL
                     LE"
     gene            4824..6404
                     /gene="cimA"
                     /locus_tag="PKK36_07370"
     CDS             4824..6404
                     /gene="cimA"
                     /locus_tag="PKK36_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNR94412.1"
                     /translation="MSKVAIYDTTLRDGAQAEGVSFSGIGKIRIAKCLDAFGIDYIEG
                     GFAASNPKDMAFFKEIKKENLQHSKIAAFGSTRRAGIAAGADAGLLALLEADTSVVTF
                     FGKSWKLHVRDVLHTTEEENCAMIADTVRFMKEHGKEVIYDAEHFFDGYRDSADYALR
                     TLQAAAQAGADMIVLCDTNGGMLPGEIARITAEVVKAVPAAVGIHAHNDSETGVANSL
                     AAIGAGAVHVQGTINGIGERCGNANLISIIPNLILKMGRECLPEGRLVKLREVAQFVS
                     EIANQRLNAKSPFVGDSAFAHKAGMHVDGVRKNPLSFEHISPEQVGNKRRVLISELSG
                     ASNVFLKAVEMGLNVDKKSPEVKNILKQLEHMEKNGYEFEAAEASFELMIQKLLKSHK
                     PFFNLEGFRVIVEKRHKDEPCISEASIKLTVKDQEAHTVGEGDGPVDALNQAMRKALT
                     AFYPSISEVTLRDYQVRILDPETATAAKTRVLIESSDGKQTWGTVGVSENLIEASWEA
                     LVDSVEYKLLLEEKNNKK"
     gene            6401..9085
                     /locus_tag="PKK36_07375"
     CDS             6401..9085
                     /locus_tag="PKK36_07375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNR94413.1"
                     /translation="MNQLEKNYDPKHIEEKWYARWLEKKYFHASSDEGGEPYCIVIPP
                     PNVTGILHMGHALNNTIQDVLVRWRRMQGRNALWVPGTDHAGIATQNVVERMLHKEKL
                     TRDHLGREAFIQRVWQWREQYGETIIGQLKKLGASCDWDRERFTMDEGLSSAVAEVFI
                     RLYEKKLIYRGNYIINWCPRCQTALSDEESEHRDQAGKLYHIRYPIKGGKRKKDYVVV
                     ATTRPETLLGDVAVAVNPRDERYGSLLEKTLELPILKRELKVIQDDFVDPAFGTGLVK
                     VTPAHDPNDFEMGRRHGLPEINVMNDDGTMNEAAGPYAGMDRFECRKKILADLEEAGL
                     IDKIEDHANAVGHCYRCHTMVEPRLSPQWFVKMKPLAGPALEAVQAGRIRFVPDRWTK
                     VYTEWMENIRDWCISRQIWWGHRIPVFYCDACGHEWAAKGQPNQCPECGDAGIRQDED
                     VLDTWFSSWLWPFSVFGWPEENEDLKFYYPTASLVTASEIIFFWVARMIMAGMEFMDD
                     IPFTDVYIHGTVRDDTGRKMSKSLGNSIDPLTIIEKYSADALRFSLIMLTATGQDVFL
                     SDDKFEIGRNFGTKIWNAARYMQMQSPELPEGFDNPEFTAGSLSPDDAHILAKLNETI
                     SACTESLERFRFNDTAKALYEFFWHQFCDWYVESSKQVLYGEDAGRKQQVLTVMHYAF
                     SSALRLLHPIMPFLTEELWTGMGYNRKHETIMRAPWPQARDDAGLEDWGIDQDTVAYV
                     DAKHDLIRVARVLMADYNLLPKHNVEFIVRPADAKLAERLKADLDSVRSLLRAGKVTV
                     DESSEPPPAMPSGLTRMGMVCMPIRGLIDTAAERARLEDQLAKVSESLQRVTRKLGNM
                     DFISRAPKDVVAKQQDMKKDLIEKSEKLGKLIATLSGE"
     gene            9092..9538
                     /locus_tag="PKK36_07380"
     CDS             9092..9538
                     /locus_tag="PKK36_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNR94414.1"
                     /translation="MKYSEAKHGRVFILRLEDGDVVHETIERFAHEHGIHAAALIAVG
                     GADEGSRFVVGPETGRGKDVNPLEHVLTGVHEVAGTGTLFPNEKGEMIIHMHMAAGRR
                     DQTVTGCIRKGVKVWHVLEVILFELLDSTAVRRFEPATGFELMQPR"
     gene            9541..10629
                     /locus_tag="PKK36_07385"
     CDS             9541..10629
                     /locus_tag="PKK36_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94415.1"
                     /translation="MSRLAYYVTAHGFGHGVRTCDILNALLEQNPGLEVELVTALPAS
                     LFSVRIRRMNRVRIRNRAFDTGMIQLDSVQVDVEATLREMQQIVSRENDLVQEEAAWL
                     RLADVRAVAADIPGIPLRAAKKAGRPAVAVGNFGWDWIYSAFAENDRRWQPVVDSFAG
                     AYAQADMLLRLPFAEEMKNFRVIEDVPLLARAGIPRRAEIAEHFGCDPSKKMVLISFT
                     SLDLEPDALARIEQIPDCEFLVVEPMNWKMKNIRPVPRDSFPFSTILASSDAVITKPG
                     FGIMSECVANGKPMIYTDRRDFIEYPVMVENINKYMRAVHIPQVELYAGNLAPFLNAL
                     DDCPPPKETLPSGGAEIAARRIMGFIKA"
     gene            complement(10801..11568)
                     /locus_tag="PKK36_07390"
     CDS             complement(10801..11568)
                     /locus_tag="PKK36_07390"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNR94416.1"
                     /translation="MTAIGRCALAAAVGLCMITGTLADDNTVTVTGKAEIKKKPDVAY
                     VTLYVKGEGILMVDAAKKADQKVEEIKMALQEQIKGILAVEVSDVAVGEAQREYWGPD
                     RKEESPRPEITRRLRITISPDPVHVYELVDVAIRAGALMQIPSTVHYSGDVRSVVVYG
                     LLDSSGVEAEARDAAMADARKEAQSLAALAGKSLGDVVKVGCGGTFPSGFPVHVMGRQ
                     ADYPTAYVGQNPSEIAVNYSLSVSFDMRKGKGDTVRE"
BASE COUNT         2461 a         3645 c         3507 g         2139 t
ORIGIN      
        1 ccataatcca gaccccagat cgcgccccaa aagcaaagtt gatgaataga tcaggataga
       61 cctgtcctcc acgaaaatca tgtccgcctc attcatgcca tcttcaagag caggtcctgt
      121 gccacgccgc atcagaaaaa agtccgccgg gaaaaaaacg tcagaggaac accggcggcc
      181 gcccgccatg ccggcctgtt ctgcggcctc cggccgcgcc cctcgaaaag tcttcggaaa
      241 atattttagt aaaacgattg acaaccggcg aagaaattca tagttaatgc aaaattcgcg
      301 ggttggattt tttgagcgtt ttttaggatt tgcgacgctt ttttgataaa aagtcccgtt
      361 ttttgggctt tttggggcaa attttggctt ttttggtcat tttttggcgt tttttggtcg
      421 ttttttgttg attttggaga ctttttatgc tttctgaacg gaaaagcggc attcttctgc
      481 atcccacgtc tcttcccggc aactggggca tcggcgagat cggacccgaa gcctgccgct
      541 tcgcggacac gctccatgaa atggggcagc acttgtggca gattctcccg atgggcccca
      601 cgagctatgg cgactccccc tatcaggctc tttccacctt cgccggaaac catctgctca
      661 tcagctttga cctgctggtg caggacggcc ttctgagcga atcgcgtctc gcccgcttcc
      721 cgaagtttga tccctcaaaa attgattacg gccccgtcat cattgagcgg atgaaggttc
      781 tgcaaacagt ctgccgctcg tttgagcgct gcgcgacgga caggatgaaa cgcgagtttc
      841 agcagttctg ccgcaggcag gcgcgctggc tggatgatta tgcactttac atggccatca
      901 aggattcgca gaacggaaag ccgtggatca agtggccgcg ggcgctggcg ggccggaagc
      961 cggaggccat gaacgaggcg gtcaagaaat tcaagacctc catccgcaac gtgaaaatcc
     1021 tgcagttcat ttttcatgac cagtggcggc ggctgaagac gtactgccac aggaagaaca
     1081 tcaagatcat cggcgacatt cccatctttg tcgcgcacga cagcgccgac gtgtgggcgc
     1141 atccggacct gtactatctg gatggccggg ggaacctgac cgtgcaggcg ggtgtgccgc
     1201 ccgactattt cagcgcgacc ggccagctct ggggcaaccc gctgtaccgc tgggacgtgc
     1261 acgcgcagtc caactacgcc tggtgggcgg cgcggctgca gcacattttc cagatggtgg
     1321 acgtggtgcg cattgaccac ttccgcggat tcgaggccta ctgggaggtg gccggcgacg
     1381 ccgagaccgc catcagcggc caatgggtca agggcccgaa ccacggcctc ttcgactcgc
     1441 tcctgcagca gctgggcccc ctgcccatca tcgccgaaga ccttggcgtg atcactccgg
     1501 aggtggagaa gctgcgcgac gacttcggct tccccggcat gcgcatcctg cagttcgcct
     1561 tcggcaacga tccccaggcg gagaacttcc gcccgcacaa cttccccgcc tcgtgcgtgg
     1621 tctataccgg cacgcacgac aacgacacca ccgtgggctg gttccacagc aagcccgacg
     1681 aaagcagcac ccgcacccag caggacatag acgaggagcg caggcgcatc ctggactatg
     1741 tgcacacgga cggaagccag attcactggg acatgatctc gctggcggcc cattcccgcg
     1801 ccaacacggc ggtggtgccg ctgcaggacg tgctgggact ggactcctcc gcgcgaatga
     1861 accgccccgg catcgccggc ggcaactggg gctggcgctt ccactgggag cagctcaccc
     1921 cggccatcat ccgccgcatg cgcgacatca cggccagcgg gaagaggtaa gagcgaaacg
     1981 gaaagcgaag tctgcgcccc cggcgggctt gccccgccgg agcgagaggt caggcagcaa
     2041 aaaaggcgat cccagccttc ttcacccgcg acacaaggtc gcgggggtcg gccaaggctt
     2101 gaagcggctt tactggtgca cgattcctga ctcctgtctc ctgtcctctc attttcctcc
     2161 gtttttactt gaaacccggg gcggttgggg taatcttttt tccattattt atgaaccgga
     2221 ccattatcaa tgtcattgta gtcaaaggcc gatgaggggc tggttgtgat tctgcgcccc
     2281 tcggaagagg ggtttttttt taactggaat gaacacatga agacttatgg agtaggcatt
     2341 ctcggattcg gcaccgtcgg agccggagtc atcgaaactc ttcagctcaa cggcgacctg
     2401 atcgccgcgc gcacggggct ccgcatcgaa gtccgcaggg tggccgacct cgacctcgac
     2461 cgcgaccgcg gcgtgaaggt ggacccctcc atcttcaccc gcgatgcgat ggccgtgatt
     2521 gacgatccgg ccgtggacat tgtggtggag ctcatcggcg gcgtgaagac cgcccgcgag
     2581 gtgatcctga aagccctgcg ggccgggaaa ccggtggtga ccgccaacaa ggccctgctg
     2641 gcggagcacg gcgacgaact ctatgccgtc gcggacgaaa gcagcgccga cctttattac
     2701 gaggccagcg tatgcggcgg gatccccgtc atccgcagtc tgcgcgaagg cctgattgcc
     2761 aaccacatag aaagcatgta cggcatcatg aacggcacct gcaactatat cctcacccgg
     2821 atggagcagg agaatcttcc gtttgagaag gtgctggcgg acgcccagac ccggggctat
     2881 gccgaagccg aaccgagcct tgacattgac ggcatcgaca ccgcccacaa ggcggcgatc
     2941 ctcgcctcgc tggcgtacgg gtttcccgtc ccgatggagg cggtccacgt ggaaggcatc
     3001 cgcggacttg atccgctgga catccgctat gcgaaggaac tcggctaccg catcaagctc
     3061 ctggcgcaga tccgccacca tgaggacggc atcgaagtgc gcgtctgccc cacccttgtg
     3121 ccgctgggac acatgctggc ctcggtcagc ggaaccttca acgccctgat ggtgcggggg
     3181 gacgtggtcg gagacaccct ctattacggg cgcggcgcgg ggcgtctgcc cacggccagc
     3241 gccgtggtca gcgatctggc cgatgtcgcc cgcaacctgg ccagcagctc caacctgcgc
     3301 gtgcccgccc tcgtgcgcca cggcgaatac ggaaagatga agaagctcga ggatctggag
     3361 tcatgctgct atctgcgcat gtccctcaag gatcacccct gtgtgctggc ccaggtggcc
     3421 caggtgctgg gcgagaacgg aatcagcatt gcctcggtga tccagaacaa gcccggcgag
     3481 ggctgcgatt acgctcccgt gattgtggtg acccaccgcg cgccggaaaa gaaattccgg
     3541 caggcgctgc ggatcataga cgcgctcgac gtggtcggcg cccgcactat ccgcatggcc
     3601 attgaggatt ttgaatagac cggatgaaga agatcatcat agtctgcgac agcgtggcgg
     3661 atgccccgct ggaggaactg gacggccgga ccccgcttca gaccgcgcgc tgcccgtttg
     3721 cctcgcggct tgcgcaaacc ggacagaccg ggctgctgac cctgccccgg gccggaactg
     3781 aagaccacgg cgaacagctt ctgggactct ttgccggcat gacccgcgaa caggcctcgg
     3841 cgctccggca gggaccgctc gaagccatgg cctccggcat tgacctctcc gactacgatt
     3901 tcgcctggtg cggacatttc gtgaccatgg acggcgacgt gctccgcgac agcctggtcc
     3961 ggtcgctttc acttgaagaa acccgatggc tcgctgaaca gcttcagcgg gaatgggatg
     4021 cgcagcgggt ccggtttgcc gccacggagc cgtcccggct gctggtgctc tattccaccg
     4081 ccggcggggc ccgtccgcag ccggggcacc ctccccatct ttccgaaggc gcccgcgcgg
     4141 acgagttcat ccggcgctca agggcggaca acatcgtgcg cgatattttc gaaagatccc
     4201 gccggatact ggatagccag ccgctgaatg aaatccgggt ggacctgggc gagaatcccg
     4261 ccaacgcgct ttggctgtgg ggcggcggcc ccatgagcgg catgcatgaa ctcttcaccg
     4321 ggtttcgcat cagcggcgcc atgctgaccc agtgtccgat ggctgccggc cttgcccgcc
     4381 tggccgggat ggaaaccatc gaaatgaggg atgtgtggac ggccggcggc ggcatggcct
     4441 tcaatcttcc gcttctggtg caggaactcc ggcagaagga tctgctgctg gtctatgcgg
     4501 gcgcgccgag acccggcgga cgctacggca gcgcggtgga aaaagtccgg gccatcgagg
     4561 ccatggatgc caatctgctg gggccgctgt cagacgtgct ggcggcgcac cgcccctgcc
     4621 ggatcatcct cgccgccaac cggggggtct ccaccccaac gggcctgccg gaagccggac
     4681 gaatcccgtt cgtgctggcc ggcgacggaa tcggagcgga cgaaacacaa aactggaatg
     4741 aagaagaagc cgcccgcggg gcgctgggca gcgtgaagct cagccggctt gcggatcttc
     4801 tggaatagcg gcaggaggaa gttatgagca aagtagccat ctatgacacc acgctgcgcg
     4861 acggagcgca ggcggaaggc gtttcctttt ccggcatcgg gaaaatccgc atcgccaaat
     4921 gcctggacgc gttcggcatt gactatatcg agggaggctt cgcggcctcg aatccaaaag
     4981 acatggcttt tttcaaggag atcaaaaagg aaaatctcca gcactccaaa atcgccgcgt
     5041 tcggaagcac gcgccgcgcc gggatcgcgg cgggcgcgga cgccggcctg ctggcgctgc
     5101 tggaggcgga tacgtccgtt gtgacctttt tcgggaagag ctggaagctg cacgtccgcg
     5161 atgtgctgca caccaccgag gaggaaaact gcgccatgat cgccgacacc gtgcgcttta
     5221 tgaaagaaca cggcaaggaa gtgatctatg acgccgagca cttcttcgac ggctaccggg
     5281 attccgccga ctatgcgctg cgcaccctgc aggcggccgc gcaggccggc gcggacatga
     5341 ttgtgctgtg cgacaccaac ggcggcatgc tccccggcga aatcgcgcgg atcaccgccg
     5401 aggtcgtcaa ggccgtcccg gcggccgtcg gcatccatgc ccacaacgat tcagaaaccg
     5461 gcgtggccaa ttcactggcc gcgatcggcg cgggcgccgt gcatgtgcag ggcaccatca
     5521 acggcatcgg cgagcgctgc ggaaacgcca acctgatttc gatcatcccg aacctcatcc
     5581 tcaaaatggg ccgggaatgc ctgccggaag ggcggctggt caagctgcgc gaagtggccc
     5641 agtttgtctc cgaaatcgcc aaccagcggc tcaatgccaa gtccccgttc gtcggcgaca
     5701 gcgcctttgc gcacaaggcc ggcatgcatg tggacggcgt gcgcaaaaat ccgctcagct
     5761 tcgagcacat cagcccggag caggtcggca acaagcggcg tgtgctgatt tccgaactct
     5821 ccggcgcaag caacgtcttc ctgaaggccg ttgagatggg cctcaatgtg gacaagaaat
     5881 cacccgaggt gaagaacatc ctgaagcagc tcgagcacat ggagaaaaac ggctatgagt
     5941 tcgaggccgc tgaagcctcg tttgaactga tgatccagaa gctcctcaag tcgcacaagc
     6001 cgttcttcaa cctcgaaggg ttccgcgtga ttgtcgaaaa acggcacaag gacgagccct
     6061 gcatctccga ggcctccatc aagctgacgg tgaaggacca ggaagcccac accgtgggcg
     6121 aaggcgacgg cccggtggat gcgctgaacc aggccatgcg caaggcgctg acggcctttt
     6181 atccgtccat cagcgaagtg acgctgcgcg actatcaggt gcgcatcctc gatcctgaaa
     6241 cggccaccgc ggccaagaca cgcgtgctga tcgagtcctc cgacgggaaa cagacctggg
     6301 gcacggtcgg cgtgtccgaa aacctgattg aagcatcatg ggaggcgctg gtggacagcg
     6361 tggaatacaa gctcctgctg gaagagaaga acaacaagaa atgaatcagc tagaaaaaaa
     6421 ctacgatccg aaacatatcg aggaaaagtg gtacgcccgc tggctggaaa agaaatattt
     6481 ccatgcctcg tctgacgagg gcggcgaacc ctactgcatt gtgattcccc cgccgaacgt
     6541 gacgggcatt ctgcacatgg gccacgcgct gaacaacacc attcaggacg tgctggtccg
     6601 ctggcggcgc atgcagggcc ggaatgccct gtgggtgccc ggcaccgacc acgcgggcat
     6661 cgcaacccag aacgtcgtgg agcgcatgct ccacaaggag aaactcaccc gcgaccatct
     6721 cggccgcgaa gcgttcatcc agcgcgtgtg gcagtggcgc gagcagtacg gcgaaaccat
     6781 catcggtcag ctcaagaagc ttggggcctc ctgcgactgg gaccgcgagc gcttcaccat
     6841 ggacgaaggc ctgagcagcg ctgtcgcgga agtgttcatc cggctctatg aaaagaagct
     6901 gatctaccgc gggaactaca tcatcaactg gtgtccgcgc tgccagaccg ctctttccga
     6961 cgaggagagc gagcatcgcg accaggccgg gaaactgtac catatccgct atcccatcaa
     7021 gggcggaaag cgcaagaagg actacgtggt cgtggccacc acacggcccg aaacactgct
     7081 cggcgatgtg gccgtggccg tcaatccgcg tgacgagcgc tacggcagcc tgcttgagaa
     7141 aacccttgag ctgcccatcc tgaagcgcga actgaaggtg atccaggacg attttgtgga
     7201 ccccgcgttc ggcacgggcc tggtcaaggt gacgcccgcg cacgacccca acgactttga
     7261 aatgggacgc cgccacggcc tccccgaaat caacgtcatg aacgatgacg gcaccatgaa
     7321 cgaggcggcg ggcccctatg ccggcatgga ccgctttgag tgccgcaaga aaatcctggc
     7381 cgacctcgag gaggccggac tgattgacaa gatcgaggat cacgccaacg ccgtcggcca
     7441 ctgctaccgg tgccacacga tggtcgagcc gcgcctgtct ccgcagtggt ttgtgaaaat
     7501 gaaaccgctg gccgggcccg ccctcgaagc cgtccaggcc ggccggatcc gctttgtgcc
     7561 cgaccgctgg accaaggtct ataccgaatg gatggaaaac atccgcgact ggtgcatttc
     7621 acgccagatc tggtggggcc accgcatccc cgtgttctac tgcgacgcct gcgggcacga
     7681 gtgggcggcc aaaggccagc ccaatcagtg cccggaatgc ggcgacgccg gcatccgcca
     7741 ggacgaggac gtgctggaca cgtggttctc ctcgtggctg tggccgttca gcgttttcgg
     7801 ctggccggag gaaaacgaag atctgaagtt ctattatccg accgcctcgc tggtcacggc
     7861 ctcggaaatc attttcttct gggtggcccg catgatcatg gccggcatgg aattcatgga
     7921 cgacatcccc ttcaccgatg tctacatcca cggcacggtc cgcgacgaca ccggccgcaa
     7981 gatgagcaag agcctcggca actccattga tccgctgacg atcatcgaga aatacagcgc
     8041 cgacgcgctc cgcttcagcc tgattatgct gaccgcaacc ggccaggatg tctttctttc
     8101 ggatgacaag tttgaaatcg gccggaactt cggcaccaag atatggaacg cggcgcgcta
     8161 tatgcagatg cagtctccag agcttccgga aggcttcgac aatccggagt tcacggcggg
     8221 cagcctgagc ccggacgacg cgcacatcct cgccaagctc aacgagacca tctctgcctg
     8281 caccgaaagc ctcgagcgct tccggttcaa cgacaccgcc aaggcgctct acgagttctt
     8341 ctggcaccag ttctgcgact ggtacgtcga gtcctccaag caggtgctct acggcgagga
     8401 tgccggccgc aagcagcagg tgctcacggt gatgcactat gctttctcca gcgcgctgcg
     8461 cctgctccac cccatcatgc cgttcctcac cgaggaactc tggaccggca tgggatacaa
     8521 ccggaaacac gaaaccatca tgcgcgcgcc ctggccgcag gcgcgcgacg acgccggact
     8581 cgaggactgg ggcattgacc aggacaccgt ggcctacgtg gacgcaaagc acgacctgat
     8641 tcgcgtggcg cgggtcctga tggccgacta caacctgctc ccgaagcaca acgtggaatt
     8701 cattgtccgg cccgccgatg caaagctggc cgaacggctc aaggccgacc tggattccgt
     8761 ccggtccctg ctgcgggccg ggaaagtcac cgtggacgag agcagcgagc cccctcccgc
     8821 catgcccagc ggactgacgc gcatgggcat ggtctgcatg cccatcaggg gcctgattga
     8881 cacggccgcc gaacgcgcgc ggctggagga tcagctcgcc aaggtcagcg aaagcctgca
     8941 gcgcgtgacg cgcaagctcg ggaacatgga cttcatcagc cgggctccga aggacgtcgt
     9001 cgccaagcag caggacatga agaaggatct catcgaaaaa agcgagaagc tcggcaagct
     9061 gatcgccacg ctgtcgggag agtgacgcgc cgtgaaatat tccgaggcaa agcatggacg
     9121 cgtttttatc ctccggctgg aggacggcga tgtggtccat gagaccattg agagattcgc
     9181 gcatgagcac ggaatccatg cggccgcgct gatcgctgtg ggcggcgccg atgagggaag
     9241 ccgcttcgtc gtcggaccgg aaaccggccg gggaaaggat gtgaaccctc tcgaacacgt
     9301 gctgacgggc gtgcatgagg tcgcgggaac cggcacactc tttccgaatg aaaagggcga
     9361 aatgatcatc cacatgcaca tggccgcggg ccgcagggac caaacggtca cgggatgcat
     9421 ccgcaagggc gtgaaggtct ggcacgtgct ggaagttatc cttttcgaac tgctcgattc
     9481 gactgccgtg cgccgatttg aaccagccac aggcttcgaa ctgatgcagc cccgctgacc
     9541 atgagcaggc tggcctatta cgtcacggcg cacggtttcg gccacggcgt gcggacctgc
     9601 gacatcctca acgcgcttct ggaacagaat cccggcctcg aagtcgaact ggtgaccgcc
     9661 ctgcccgcct cgcttttttc cgtccgaatc cgccgcatga accgtgtgcg catccgcaac
     9721 agggcatttg acacgggaat gatccagctt gattccgttc aggtggacgt ggaagcaacg
     9781 ctccgtgaaa tgcagcagat tgtttcacgc gaaaatgacc tggtgcagga ggaagccgcg
     9841 tggctgcgtc tggcggatgt ccgggccgtc gccgcagaca tacccggcat tccgctccgg
     9901 gccgcaaaaa aggccggccg gccggcggtg gccgtcggca atttcggctg ggactggatc
     9961 tattccgcat tcgcggaaaa cgaccggcgc tggcagcccg ttgtggactc ctttgccgga
    10021 gcctacgcgc aggcggatat gctgctgcgc ctcccgttcg cggaggagat gaagaatttc
    10081 cgcgtgatcg aggatgttcc cctgctggcc cgcgcgggaa ttccacgcag ggcggaaatc
    10141 gccgaacact tcggatgcga tccctcaaaa aaaatggtgc tgatatcttt cacatcgctc
    10201 gatctggaac ccgatgcgct ggcgcggatc gaacagattc cggactgcga attcctcgtc
    10261 gtcgagccga tgaactggaa aatgaaaaac atccggccgg tcccgcgtga cagttttccg
    10321 ttttcaacaa tacttgcctc atcggacgcc gtcatcacca agccggggtt cggcatcatg
    10381 tccgaatgcg tggcaaacgg caaaccgatg atctacaccg accgcaggga tttcattgaa
    10441 tatccggtca tggtggagaa cataaataaa tacatgcgcg ccgttcacat tccgcaggtt
    10501 gagctctatg caggaaatct tgcaccattt ctcaacgccc ttgacgactg cccgcctcca
    10561 aaagagacac tcccctctgg cggggcagaa atcgccgcgc ggcggatcat gggctttatt
    10621 aaagcctgat ccctccgcga agtaaaactg caagcccgca aaggcctcca tcccgcgccg
    10681 ctttctgccc ccggcaggct tgtcccgccg gagcggaagg tcaggtcaac ccaatataga
    10741 accgcaaatc agcgcgaata aacacacatg tgaacgggaa aagcattagc ccgtctttcg
    10801 ctattcacgg actgtatcgc cttttccctt tctcatatca aacgaaacgg aaagcgaata
    10861 attcaccgcg atttcggagg gattctggcc cacataggct gtcggataat cggcttgacg
    10921 ccccatgaca tggacaggaa acccggatgg aaacgttccg ccgcagccga ccttgaccac
    10981 atcccccagc gacttcccgg ccagagccgc cagactctgc gcctcttttc tggcgtctgc
    11041 catggccgcg tcgcgggcct cggcttcgac accggaagaa tcaagcaggc cataaacgac
    11101 cacactgcgc acgtccccgg aatagtggac ggtcgaagga atctgcatca gggcgccggc
    11161 ccggatggcg acatccacca gctcatacac atgcaccgga tcaggcgaaa tggtaatacg
    11221 aagacggcgg gtaatttcag ggcggggaga ttcctctttc cgatcgggtc cccagtattc
    11281 gcgctgggcc tcgcccactg caacatcgga aacttcaacg gccaaaattc ccttgatctg
    11341 ctcctgcagg gccatcttga tttcttccac tttctggtcg gccttcttcg ccgcatccac
    11401 catcaggatc ccctcgcctt tcacatacag ggtgacgtaa gcgacgtccg gcttcttctt
    11461 gatttccgcc ttgcccgtga ccgtgaccgt gttgtcgtcc gcaagcgtgc cggtgatcat
    11521 gcaaagacca acggccgccg ccagtgcgca tctgccgatt gctgtcatat ctgtctcctt
    11581 ttccgctgtc ctttaagtcg ccatactatg tttgttgaaa ccggaatgca catcaaaatc
    11641 acaagtttag aatcaaacgc cggtttttct gttacggcat ctgacagcct gtcattgcca
    11701 acctgcaggc gcttagcttt ccatgtgcgt tatttcgggc aacagatgcc cc
//
LOCUS       DAOQWR010000079        11729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_5940_length_11729_cov_5.490920, whole genome shotgun sequence.
ACCESSION   DAOQWR010000079 DAOQWR010000000
VERSION     DAOQWR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_11729_cov_5.490920"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKK36_07395"
     CDS             <1..418
                     /locus_tag="PKK36_07395"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNR94417.1"
                     /translation="PLVDLLTPNIPEAQMLVGHPVDDLPSAAAEILALGAKSVLIKGG
                     HASGEESRDYWTDGSDSAWFSAPRITGREFRGTGCALSSAIAAGIAQGLPLNTAVAEA
                     KNYVTEAMKRSATCVQGKWILAAGSARPLLRQKPHA"
     gene            complement(392..1543)
                     /locus_tag="PKK36_07400"
     CDS             complement(392..1543)
                     /locus_tag="PKK36_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94418.1"
                     /translation="MRIRSGIIMLFLCVPAAARADDAQGHAYDGTIRMHASPFAPDTH
                     APTEEKKPIAPSIGRRGFSPAPTTAPLATEIRPSMTLPPPPDRTMDTETRRKENWITP
                     SSPAAEQTEAPTESGWGWLADQTARKLAEQQRIREEPADQKDDSGRAAPVQKEDRQTV
                     RDPTFFMSSDYFNNAGMFDTAAQFNTPTEEGEPDTAQPGGSDMIKAAVDHLDVATGST
                     ARQDETEELQDEQRGTDRKLTSRPDSTGIQAGPDKRPVAGYNLRPEITTPRQSLSPVA
                     FSPSSFEAPVTPSAVRIPAPAAGGAAGFSRIAAPSAPTAPAGITRPTGFSGSDSPLTR
                     PSLMPAHGAAPVSSAGMPPAGNIQTPVARPAAKSLYDAREESGMRLLPQ"
     gene            1651..3123
                     /locus_tag="PKK36_07405"
     CDS             1651..3123
                     /locus_tag="PKK36_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR94419.1"
                     /translation="MKKWMLILALLAPRPAGAYDLRLDNQRISLKAEREDIRSILGGF
                     SAAGVAVKLDPQIKAVVSVSIEDEEIPEAFEELLSPWSYVLVWNVVQTPMGELPLLSE
                     VRVHKPGRKDQVKDLKTIRSLQISTGPNGEKFVDGELLLSVRSGMTLEAFRHLLHQIG
                     GTVVESIPELGVYRVRFVFGTNIPALVEQLRLNAGVHTAEPNYITEVPSPVFRELLSA
                     AAPDPAAAPPPAGAPALAILDSGLMDISGLNAAVRGTYDAVNPSSRIGDSQGHGTQMA
                     LIASGAVLPGGVNAEADAAGVPLVAIRAFNDDGVATEFSLLRSIAYAAEQGARVINMS
                     WGSETSSSFMANAMAYAQSKGIILVAAAGNTPTGRPVYPAAYEGVIAVGAVTGSGERW
                     PQSNHGDFVSLAAPGTAEMPVGHEGPAGSYAGTSISSAFVSRALSLYLGKNPECSNAA
                     AVADLMASLRDAGEEGRDRFYGNGVLDSAALAAFLGQPCR"
     gene            complement(3108..3464)
                     /locus_tag="PKK36_07410"
     CDS             complement(3108..3464)
                     /locus_tag="PKK36_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94420.1"
                     /translation="MQTMEVSQLNIPALAAWALLGLVVLISLAATVMNILRAKSKRER
                     AFTLQSCLVVWLLLISLVLTALYVEAPYKYVALFALIVLFPLVVYHAATRRQLLQILE
                     EREKHLGKNLDSQRQG"
     gene            3604..4269
                     /locus_tag="PKK36_07415"
     CDS             3604..4269
                     /locus_tag="PKK36_07415"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94421.1"
                     /translation="MKNAQWLIVFTVLSLVALMCGCDIESAESSQNEVGINVEGFYAN
                     SGGRLVQQNTGNPITSMNLRQNGDGLEGVDNNGNLFKGTIGQVIEGGTASFNLTGLTT
                     AGNEGTISGNFTVSGTSSTMSGTWIEDAFYSTVYGTATVPNNTNDTGGVTYRLTVNVS
                     PAGTGTVSPGTGNYAANSTVTLTATAATGHTFSSWSGVSSSSGNSATVTMNGDRTVTA
                     NFN"
     gene            complement(4271..4930)
                     /locus_tag="PKK36_07420"
     CDS             complement(4271..4930)
                     /locus_tag="PKK36_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94422.1"
                     /translation="MMKHVLTVLIAAVALETGATAGDFERYRVIMDRKPFGEAPPEAE
                     VVREIPLSESFAKSLRLCAITTEEDGRIRAGIIDDATQNYYRLFEGESEDGMELVSVD
                     PESQQAVMQKGNEMAVVSMKSSDNKPLNQAQQQEQMAAREQRRLSYAERRAARRAEAE
                     ARKQQPPPESQFKTQEALQQHLQQYQMEVIRQGLPALPIPLTQEMDDQLVAEGVLPPI
                     E"
     gene            complement(5010..6647)
                     /locus_tag="PKK36_07425"
     CDS             complement(5010..6647)
                     /locus_tag="PKK36_07425"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNR94423.1"
                     /translation="MLKKEVIHADVVSVGAGVASLSTAIRLLKRSREAGSSRGKPSVL
                     IVEKAKQAGSHVLSGAIINPEPFRRLLGPDEMASLPLDAVVRGESMYFLAGRGQIRLP
                     FVPPPMSSRGMPTASLARLTARLAEICESLGGEIYTDVPAVDLLRNGSGRVTGIRTGD
                     KGSDRNGLPKTNFTPGEEIHAHMTVLGEGACGTLTDKLIQDCRIKTPSDGQMYALGIK
                     ELIEIPSRPDLCGRTIHTFGWPLGFRTFGGGFLYGLRYNLLAVGLVIGLDYRPAEADL
                     HHLFRRFKQHPLVSRWLEGGKIVEYGAKILPEAGWFSVPQLTADGALIVGDAGGLLDA
                     VRLKGVHLAVESGLAAGDALYDCWRDGDWAAAKTAAYPAALEATQAWKDLKQFRNARP
                     AFQRGLLAGMAAIGAGWLTRGLLPPGRIGHRADHEGMKRSAGRVPPPLRVRGNTPDAD
                     ILTDLFYSGTGHEEDQPCHLIIRDPEICRTQCRPKFNAPCTRFCPAQVYEWDEAAAAI
                     RISPSNCVHCKTCQIKDPLQNIEWRLPESGGPNYRNM"
     gene            complement(6641..7432)
                     /locus_tag="PKK36_07430"
     CDS             complement(6641..7432)
                     /locus_tag="PKK36_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNR94424.1"
                     /translation="MMKGKQHAGIEGEEAFINGVRARINELLAAELPDFSRIPEGGKM
                     LRARLVYAVGGRNPDAAAAVELMHGGSLLHDDIIDNGILRRGRPAFWKTHGAKGAILL
                     GDMHFFLALKLLNGRPQMQQDLIEMAGMVCKAAAEEELCGASARYLELARQKTGPLFA
                     FAASAGAPDTQSRAALREAGFLLGTAYQLADDIYDATGEVQRADKSLRRDRMKSPASV
                     QTIAALCKESAMRLQDWPDLQRAWEEYLERQFEPAIKVFTSGASC"
     gene            7583..7870
                     /locus_tag="PKK36_07435"
     CDS             7583..7870
                     /locus_tag="PKK36_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94425.1"
                     /translation="MPSASEQRSGVDCHGQCEHCHLMPSDVKGGLSGGRLVLASAGVF
                     IFPLVLAIAGGILCRANPDHQALGVVAGLAAGGLIASLAVKRLYWRFGETK"
     gene            7867..9219
                     /gene="rsxC"
                     /locus_tag="PKK36_07440"
     CDS             7867..9219
                     /gene="rsxC"
                     /locus_tag="PKK36_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNR94426.1"
                     /translation="MSTALQERPGTKTFARGIHPAENKELAEHQTIEVVPSPAEVLIP
                     LLQHVGPPCTLAVKTRDEVSVGTKIAASEAFISAPIHATIDGIVGRPTVATLPNGRHV
                     EAVPIKAGTQKNTPEEIKRDFLGGEWSTANLERFTSEQIVAAVREAGIVGQGGAGFPT
                     SVKIALNPKKPVDTVLVNGCECEPYLTADHRLMLESPSSIITGALLTARASGADRVLI
                     AIEDNKPDAVAAMEKAATGTPVKIVVLKTKYPMGGEKQTILSSTGRVVPTGGLPLDVG
                     VVVVNVASASAIARAVLRNKPLTHRIVTVTGAGIRSPKNLLAPIGISYGELIEFCGGL
                     TPDAKRIVAGGPMMGFTLGSLATPVTKGTSGITVMTSADLAKSEETACVRCGRCVDVC
                     PLNLVPTKMALAARHHDWDLAKRYHILACMECGCCGYACPANIPLVQLIRMGKAKARE
                     "
     gene            9237..10241
                     /locus_tag="PKK36_07445"
     CDS             9237..10241
                     /locus_tag="PKK36_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNR94427.1"
                     /translation="MSEEQQNQTEVAPAPSLEVAPGPHLHTTKLSTRRMMADVLIALV
                     PLVVAALAVFRMGAVMQIVVCTAAALVAEYLFTAMRGKPFSLGDGSAAVTGLILALSL
                     PGTAPWYVGAIGSFSAIGLGKVVFGGLGQNVFNPAMVGRAFVMIAFPAAVGASAYVDT
                     SSVVEAVTRATPLTALKQEGLPTSLADLFIGSTNGSLGETSALACLIGGLYLCIRRTA
                     SWEIPAGVIAAVAVIAGLINLTDMQAHWTVAHHILGGALLFGAFFIATDPVSSPLTPK
                     GKWIFGAGVGALVMLLRKLSGYPEGVMFAVLIMNALVPLINRWTIPVPVGGPVPEPKS
                     "
     gene            10251..10802
                     /locus_tag="PKK36_07450"
     CDS             10251..10802
                     /locus_tag="PKK36_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNR94428.1"
                     /translation="MKDNYLKQAWLVLLLALCFGGVLAGVEHSLKGRIDENKKADTLG
                     QIPNLVPGAVSASFSNGVYRAVTQNGDHAGWVLLVTGQGFADKVELLLGLNADASQIT
                     GVYILDQKETPGLGNKIVEDEWRAQFAGKSTQQPLEIVKRKAEKDNEIEAVTGATISS
                     ETVKDMVNRAAADFRRQLASGGF"
     gene            10805..11440
                     /gene="rsxE"
                     /locus_tag="PKK36_07455"
     CDS             10805..11440
                     /gene="rsxE"
                     /locus_tag="PKK36_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HNR94429.1"
                     /translation="MTEKGPTPLERFINGILPENPVYRQVLGMCPTLAVTNGMKAAMT
                     MAGAVAFVLVCANILTSLIRNLLKPHLRILVFTLTIATFVTVADRIIAAYMYDMSKQL
                     GPYIPLIIVNCIIICRCEVCASKQNVLTAATDALGQSLGFALALASIASVREILGSGS
                     WFGIEVMPAGWPDWVVMVLPPGAFLTLGLLLGAANWIGELAARRRTTQGVA"
     gene            11440..11658
                     /locus_tag="PKK36_07460"
     CDS             11440..11658
                     /locus_tag="PKK36_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNR94430.1"
                     /translation="MEYLSTLILIALSAALINNFVLHYFVGICPFIGVSRRVDMAFGM
                     GCAVTFVISIAALLSWLLTYYVLREGDR"
BASE COUNT         2234 a         3466 c         3569 g         2460 t
ORIGIN      
        1 cccgcttgtt gatctgctga ccccgaacat tccggaagcg caaatgctgg tggggcatcc
       61 ggtggatgat ctgccttccg ccgcagcgga gattctggct ctgggagcga agtctgtgct
      121 gatcaagggg gggcatgcct ccggagaaga aagccgtgac tactggacgg acggatcgga
      181 ttcggcctgg ttctccgcgc cccgcatcac cggccgtgaa tttcgaggaa caggatgcgc
      241 gctgtcatca gccatcgccg ccggcatcgc gcaggggctg ccgctcaata ccgctgtcgc
      301 cgaggccaaa aactatgtga cggaggccat gaagcggtcc gcaacgtgcg tgcaggggaa
      361 atggattctt gcggcaggtt cagcccggcc cctattgcgg cagaagccgc atgcctgact
      421 cttcccgcgc gtcataaagc gatttcgccg cgggtcgtgc gacaggagtc tggatatttc
      481 cggccggcgg cattcccgca gaggatacgg gcgcggcgcc atgcgccggc atcagcgacg
      541 gccttgtcag cggactgtcc gatcccgaaa aacctgttgg ccgggttatt ccggccggcg
      601 ccgtcggcgc ggacggcgcc gcgatccggc tgaatcccgc cgctccgcca gccgcggggg
      661 cggggatgcg gaccgccgaa ggcgtcacag gagcctcgaa tgaagacgga gagaacgcga
      721 cgggggacag ggactgccgc ggagtagtga tctcaggcct caggttgtat cctgcaaccg
      781 gtcttttatc cggccctgcc tgtatgcctg tggaatccgg cctggatgtc aattttcgat
      841 ccgttccccg ctgttcatcc tgcagttcct ctgtttcatc ctgacgtgcc gttgaaccgg
      901 tggccacatc cagatggtca acagccgcct tgatcatgtc gctcccgccg ggctgcgcag
      961 tgtctggttc tccttcttcg gtcggagtgt tgaactgggc cgccgtgtcg aacatgcctg
     1021 cgttgttgaa atagtcgctg ctcatgaaga aggtgggatc gcggactgtc tgacggtcct
     1081 ctttctgcac gggggctgcg cgtccggagt catctttctg atctgcgggc tcctccctga
     1141 tgcgctgctg ttccgcaagt ttccttgctg tttgatccgc cagccagccc cagccggact
     1201 ccgttggcgc ctctgtttgc tctgcggccg gtgacgaagg ggtgatccag ttttccttcc
     1261 tgcgcgtttc tgtgtccatc gtgcggtcag gcgggggagg aagagtcatg gacggacgga
     1321 tttccgtcgc cagcggcgcc gtggtcggcg caggactaaa ccctctgcgc ccgatggatg
     1381 gcgcgatggg tttcttttcc tccgtcggcg cgtgagtgtc cggagcaaaa ggcgaggcat
     1441 gcatccgaat ggtgccgtca tacgcatgac cctgtgcgtc gtccgcccgt gccgcggccg
     1501 ggacgcacag gaacagcatg ataatgccgg atctgattct catactatga ggataccatg
     1561 tgtttccggt ctcgtcaaaa atcaaaaggc ttccgggtaa aacagcgccg tgctttgatt
     1621 tgaccggcgg gatgtgatag acttgtcgca atgaaaaagt ggatgcttat tctggctttg
     1681 ctggcaccgc gcccggcagg ggcttatgat ctgcggctgg acaaccagcg gatcagcctg
     1741 aaagcggaac gcgaggacat ccgctccatc ctcggcggat tttccgccgc gggcgttgcg
     1801 gtcaagctgg atccgcaaat caaagcggtc gtgagtgtat ccatcgaaga tgaggaaatt
     1861 ccggaagcgt ttgaggaact tctgagcccg tggagctatg tgctggtgtg gaatgtggtg
     1921 cagacgccaa tgggcgaatt gccgctcctg tccgaggtcc gcgtgcacaa gcccggccgg
     1981 aaggaccagg tgaaggacct caaaacaatt cgcagcctgc aaatcagcac cggcccgaac
     2041 ggcgaaaaat tcgtggacgg cgagcttcta ctgtcggtcc ggtccggcat gacgctggag
     2101 gccttccgtc acctgcttca tcagattggc ggaacggtgg tggaatccat ccccgaactg
     2161 ggagtctatc gagtgcggtt tgtgttcggc acgaatatcc ccgcactcgt tgaacagctt
     2221 cgcttgaatg ccggtgtgca cacggccgaa ccgaattaca ttaccgaagt tccttctccg
     2281 gttttccggg aactgctttc agccgctgcc cccgatccgg ccgccgcgcc gccgccggcg
     2341 ggcgcgcccg cgctggccat tctcgacagc ggcctgatgg atatcagcgg gctgaacgcc
     2401 gccgtgcgcg ggacgtatga tgcggtgaat ccatcatcgc gaatcggcga cagccagggg
     2461 cacggcaccc agatggcgct gattgccagc ggagcagtcc tgccgggggg cgtgaacgcg
     2521 gaagcggatg ccgcgggcgt tccgctcgtc gccatccggg ccttcaatga tgacggcgtg
     2581 gccacagaat tttcgctcct gcgaagcatt gcctatgcgg cggagcaggg cgcgcgcgtg
     2641 atcaacatga gctggggctc ggaaaccagc agcagtttca tggcgaatgc catggcctat
     2701 gcgcagtcga agggcatcat tcttgtggcg gccgcgggca acacgccaac aggccgcccg
     2761 gtctatcccg ccgcttatga gggcgtgatt gccgtcgggg ctgtcacggg gagcggcgag
     2821 cgctggcctc agtcaaatca tggtgatttt gtgtctctgg cggccccggg cacggcggaa
     2881 atgccggtgg gtcatgaggg cccggccgga tcgtatgcgg gcacatccat atcaagcgcg
     2941 tttgtctcgc gcgccctgtc cctgtatctg ggtaaaaatc cggaatgctc caatgcggct
     3001 gcagtggccg atctgatggc gtcgctccgg gatgcggggg aggaaggccg tgaccgtttt
     3061 tacgggaatg gcgtgctgga ttccgctgcg ctggcggctt ttcttggtca gccctgccgc
     3121 tgactgtcca gatttttccc cagatgcttc tcgcgctcct ccagaatctg cagcagctgc
     3181 cgccgcgtgg cggcatgata gaccaccagc ggaaacagca caatcagggc aaaaagcgcc
     3241 acatatttgt acggagcctc gacatacagc gcggtcagaa ccagtgaaat cagcagaagc
     3301 cagacgacga gacaggactg aagcgtgaac gcccgctctc gtttgctctt cgcgcgcagg
     3361 atattcataa ccgtcgccgc caggctgatg agcacaacga gcccaagaag agcccaggcc
     3421 gccagagcgg gaatattcaa ctgtgatact tccattgtct gcatagacaa agccctcaaa
     3481 atgcgtcatc aatgaacaaa atcccgggca ttgtgtcaat ccaaacaaaa ccggtgcttg
     3541 catccggctg ggcgaaaatc taatctggcg gccggaaatg gaaccgaaaa tgaggatgtg
     3601 actatgaaaa acgcgcaatg gctcatcgtt tttaccgttc tttcgctggt cgctttgatg
     3661 tgcggatgcg atatcgaatc cgccgagagc agccagaacg aggtaggaat caatgttgag
     3721 ggcttctatg ccaactccgg aggccgcctg gttcagcaaa acacgggcaa tcccatcacc
     3781 agcatgaatc tgcgccagaa cggcgatggc ctcgagggcg tggacaataa cggcaacctt
     3841 ttcaagggga ccatcgggca ggtgatagaa ggcggaacgg cttcattcaa cctgacgggc
     3901 ctgacgacgg cgggcaatga gggaaccatt tccgggaatt tcactgtcag cggcacttcc
     3961 tcgaccatga gcggaacctg gatcgaagat gccttctatt ccactgtcta tggaactgcg
     4021 acggttccga acaacaccaa cgacactggc ggcgttacct acaggctgac tgtcaatgtt
     4081 agtccggctg ggacgggaac tgtaagtccg ggtaccggca actatgccgc aaactcaacc
     4141 gtcacactga cggcaactgc agcaacgggc catacatttt cgagctggtc gggagtgagt
     4201 tcttcttcgg gaaattcggc cacagtgacg atgaatggcg accgcaccgt gacggccaat
     4261 ttcaactgat ctattcaatc ggcggcagaa cgccttcggc gacgagctgg tcgtccattt
     4321 cctgcgtcag cgggatgggc agggcgggga ggccctgacg gatcacttcc atctgatact
     4381 gctgcaaatg ctgctgaagg gcttcctgcg tcttgaactg ggattccggc gggggctgct
     4441 gtttgcgcgc ttcagcctcc gcccggcggg ccgcccgccg ctcggcatag gacagccgcc
     4501 gctgctcgcg ggccgccatc tgctcctgct gctgcgcctg gttcagcggc ttgttgtcgg
     4561 atgacttcat gctcaccacg gccatctcgt ttcccttctg cataaccgcc tgctgggatt
     4621 ccggatcaac cgagaccagt tccatcccgt cctcggattc cccctcgaaa agccggtagt
     4681 aattctgggt ggcgtcatca atgattcccg cgcggatgcg cccgtcctcc tcggttgtga
     4741 tggcgcacaa ccgcaggctc ttggcaaacg attcgctcag gggaatttcc cggaccactt
     4801 cagcctcggg aggcgcctcg ccgaacggct tgcggtccat gatgacgcga taacgctcaa
     4861 aatcacccgc agtcgcaccg gtttccagcg cgacggccgc aatcaggact gtcaggacat
     4921 gtttcatcat tctcaattca agcatactcg gcttcatggc tgcgtcaagt ggtcagactg
     4981 cgcgagccct gcgtttgttc attgcatttt cacatattgc ggtaattcgg cccgccgctt
     5041 tccggaagcc gccattcgat gttctgcagc ggatccttga tctggcaggt cttgcagtgc
     5101 acacagttgg aaggagaaat ccggatcgca gcagcggcct catcccattc atagacctgc
     5161 gccggacaga accgggtgca gggggcattg aactttggcc ggcactgtgt gcggcagatt
     5221 tccgggtccc tgatgattaa atggcacggc tggtcttctt catgcccggt gccggaataa
     5281 aacagatccg tgagaatgtc ggcatccggc gtgttgcctc gaacccgcag cggcggcggg
     5341 acgcggccgg ctgaacgctt catgccctca tggtccgccc ggtgtccgat gcggcccggc
     5401 gggagcagtc cgcgggtgag ccagcctgct cctatggcgg ccatccccgc aagcagtccc
     5461 cgctgaaatg ccggccgggc attgcggaac tgcttcaggt ctttccatgc ctgtgttgct
     5521 tccagcgcgg ctggataggc cgcggttttg gccgccgccc agtcgccatc cctccagcag
     5581 tcgtaaagcg catcgcccgc cgccaagccg gattccacag ccagatgaac gccttttaaa
     5641 cggacagcgt ccagcagtcc gcccgcatca cccacaatca gcgccccatc ggcggtgagc
     5701 tgcgggacag agaaccatcc ggcttcgggc aggatttttg cgccgtattc aacgattttc
     5761 ccgccctcca gccaccggct gacgagcgga tgctgcttga accggcggaa gagatggtgg
     5821 aggtctgctt ccgccggacg atagtcaagg ccgatgacaa ggccgacagc caacaggttg
     5881 taacgcagcc cgtaaagaaa gccgccgcca aaagtccgga accccagcgg ccagccaaag
     5941 gtatggatgg ttctcccgca aagatccggc cgcgagggga tttcaatcaa ttccttgatg
     6001 cccagcgcat acatttgacc gtctgaaggt gtttttatcc tgcagtcttg gatcagtttg
     6061 tctgtcagcg ttccgcaggc cccttcaccc agcacggtca tatgcgcatg gatttcctcg
     6121 cccggggtga aattggtttt tggcaggccg tttctatcgc tgcccttgtc cccggtccgg
     6181 attcccgtga cacgtcccga tccgttccga agaaggtcca cggcgggcac gtctgtatag
     6241 atttctcctc ccagtgattc acaaatttca gccaagcggg cggtcagccg ggcaagggat
     6301 gctgtgggca tcccccgcga actcatgggg ggcgggacaa agggaagtct gatctgtccc
     6361 ctgccggcaa gaaaatacat gctttcgccg cgtacaaccg catccagcgg aagtgacgcc
     6421 atctcgtccg gaccgagcag ccggcgaaac ggttcaggat tgataatggc tccggaaaga
     6481 acatgagatc ctgcctgttt tgccttctca acaatcagaa cggacggctt gccgcggctg
     6541 ctgccggcct cccggctccg tttcagaagg cggattgcgg tcgacagtga tgcgactccg
     6601 gcgcccacgg agaccacatc ggcatgaatg acctcttttt tcagcatgag gcccctgagg
     6661 tgaaaacttt aattgcgggt tcaaactgcc gttccagata ttcctcccat gctctctgca
     6721 ggtccggcca gtcctggagg cgcatcgcgg actccttgca gagcgcggca attgtttgaa
     6781 cggacgcagg ggatttcatc ctgtcgcgcc ggagactctt gtcggcccgc tgcacttcgc
     6841 cggttgcatc atagatatca tccgcgagct ggtaggccgt gcccagcagg aatcccgctt
     6901 cgcgcagggc cgcccggctt tgtgtatccg gcgcgcccgc ggatgccgca aatgcaaaca
     6961 gcggcccggt cttctgcctg gcaagttcca gatatcttgc ggatgcccca caaagttctt
     7021 cttcggcggc ggccttgcag accatcccgg ccatctcaat gagatcctgc tgcatctgcg
     7081 gccttccgtt cagcagtttc aatgccagaa agaaatgcat atcgcccaga agaatggccc
     7141 ccttggcgcc gtgtgttttc cagaatgcgg gccggccgcg ccgcagaatg ccgttgtcaa
     7201 tgatatcatc gtgcagcagg cttcccccgt gcatcagctc cacggcggcc gcggcatcgg
     7261 gattgcggcc tcccaccgca taaaccagcc gggcacgaag cattttcccg ccctccggga
     7321 ttctgctgaa atcaggcagt tcggcggcca gcagttcatt gatgcgggct ctgactccgt
     7381 taatgaaagc ctcttcgcct tctatccctg catgttgttt tcctttcatc attcaggaca
     7441 atatgcactt gtgtgtgatc agcgtcaacc gcattgcccg gcgcgcccgt atctccgcca
     7501 acttccggcc ttatccttct ttacagcgtc cggcgcgact ggtaatgttt gcggccgttt
     7561 gatgagatat ggaacagatg atatgccttc agcatccgaa cagagatcag gcgttgactg
     7621 ccatggacag tgtgaacact gtcatctgat gccttcagat gtgaaaggcg gcctttccgg
     7681 ggggcgactg gttctggctt ctgcgggcgt gttcattttc ccgcttgttc tggccattgc
     7741 gggagggatt ctgtgccgcg ccaatcctga tcatcaggca ctcggggttg tggcgggact
     7801 ggcggcgggc ggcctgatcg ccagccttgc cgtcaaacgg ctttattgga gatttggaga
     7861 aactaaatga gtacagcatt gcaagagcgg cccgggacca aaacgtttgc ccgtggcatc
     7921 catccggccg agaacaagga gcttgcggag catcagacca tcgaggtcgt tccatctccc
     7981 gcggaggtgc tgatccccct gctgcagcat gtgggtcctc cctgcacact tgcggtgaag
     8041 accagggatg aagtctctgt cggcacgaaa atagccgcgt ccgaagcctt catctccgct
     8101 ccgattcatg caaccattga cggaattgtg ggccggccga cggtcgcgac acttcccaat
     8161 ggccgccatg ttgaagccgt tccgatcaag gcgggcacgc agaagaatac accggaagaa
     8221 atcaagcgtg attttctggg cggagaatgg tccacggcca atcttgagcg cttcacctca
     8281 gagcagattg tggccgcagt ccgcgaagcg ggcattgtgg ggcagggcgg cgcgggtttc
     8341 ccgacctctg ttaaaattgc ccttaatccc aaaaagcccg tggatactgt gctggtcaat
     8401 ggatgtgaat gcgaacccta tttgacggcc gaccaccgtc ttatgctgga atctccatcg
     8461 tcaatcatca ccggcgctct gctcacggcc cgggccagcg gcgcggatcg cgtgctgatt
     8521 gccatagagg acaacaagcc cgatgccgtg gccgccatgg agaaagcggc tacgggaaca
     8581 cccgtgaaga ttgttgtgct caaaacgaaa tatcccatgg gcggcgaaaa acagacgatt
     8641 ctgtcctcga ctggccgcgt ggtgccgacg ggcggtcttc ctctcgatgt gggcgtcgtg
     8701 gttgtgaatg tggcctcggc ctcggcaatt gcccgtgctg tgcttcgcaa caagcctctg
     8761 acgcaccgca tcgtgacagt aacgggtgcg ggcatccgca gtccgaaaaa ccttctggcg
     8821 cccatcggca tatcctatgg cgaactgatt gaattctgcg gcggcctgac tccggatgcg
     8881 aagcggattg tggccggcgg gcccatgatg ggtttcactc ttggcagtct ggccacgccc
     8941 gttaccaagg ggaccagcgg catcacggtg atgacctccg ccgacctcgc aaagagcgag
     9001 gaaaccgcct gcgtccggtg cggccgctgt gtggacgtgt gcccgctgaa cctggtgcca
     9061 accaaaatgg cgctggcggc gcggcatcat gactgggacc tggcaaagcg ctaccacatt
     9121 ctggcctgca tggaatgcgg ctgctgcggg tatgcctgcc ccgcgaacat tccgctggtg
     9181 cagctgatcc gcatgggcaa ggccaaggcc cgcgaatagt tcaacaggag attaccatgt
     9241 ccgaagaaca gcagaaccaa acggaagtgg ctccggcccc gtcactcgaa gtggcgcccg
     9301 gcccgcatct gcataccaca aaactgtcca cccgccggat gatggcggat gtgctgattg
     9361 ctctggtgcc gcttgtggtc gccgcgctgg cggttttccg catgggcgct gtgatgcaga
     9421 ttgtggtttg tacggcggcc gccctcgtcg cggaatacct tttcaccgcc atgcgcggaa
     9481 aacccttttc actgggcgac ggctccgcgg ctgtgaccgg tctgattctt gcactttctc
     9541 ttccgggaac ggcgccctgg tatgtcgggg ccatcggatc cttctcggcg atcggcctgg
     9601 gcaaggtggt ttttggcggt ttggggcaga atgttttcaa tccggcgatg gtcgggcgcg
     9661 cgtttgtcat gatcgctttc ccagccgccg tgggggcctc ggcctatgtt gacacgtcat
     9721 cggtggttga agcggttacc cgtgccaccc cgctgaccgc gctcaagcag gagggccttc
     9781 ccacctcgct tgcggatctt ttcatcggca gcaccaacgg atcgctgggg gagaccagcg
     9841 ctctggcctg tcttatcggg ggactatatc tctgtattcg ccgcacagcc tcgtgggaaa
     9901 tcccggccgg cgtgattgcg gccgtggcgg tgatcgccgg gctgattaat ctgaccgaca
     9961 tgcaggccca ctggacggtc gcgcatcata ttctgggcgg cgcgcttctc tttggcgcgt
    10021 ttttcatcgc gacggacccg gtgagcagtc ccctgacccc caaaggcaag tggatattcg
    10081 gtgccggggt gggggctctc gtgatgctcc tgcgcaagct gagcggatat ccggaaggcg
    10141 tcatgttcgc tgtgctgatc atgaatgccc tggtgccgct gatcaaccgg tggacaattc
    10201 cggtgcccgt gggcggtccg gtgcccgagc cgaaaagctg aggaaaaaac atgaaagaca
    10261 actatctcaa acaggcctgg ctggtgctgc tcctggcatt gtgcttcggg ggggtgctgg
    10321 caggggtgga gcattccctc aagggccgca ttgatgaaaa caagaaggcg gatactctgg
    10381 ggcagatccc gaatctggtc cccggtgcgg tctcagcgag tttttcaaac ggagtctacc
    10441 gcgcagtgac gcaaaacgga gaccatgccg gctgggttct gctcgtgacc ggccagggct
    10501 tcgccgacaa agtggaactg ctgctggggc tcaatgcgga cgcttcgcag attaccggag
    10561 tctatattct tgatcagaag gaaactcccg gactcggcaa caagattgta gaagacgaat
    10621 ggcgcgcgca gtttgccgga aagagcacgc agcagccgct ggagattgtc aagcggaagg
    10681 cggaaaagga caatgaaatc gaagctgtta ccggcgcaac aatatcctcg gaaaccgtca
    10741 aggacatggt gaaccgcgcc gcggcggatt tccgccgaca gcttgcttca ggagggttct
    10801 gattatgacc gaaaaaggac cgacacctct cgaaagattc atcaacggaa ttctcccgga
    10861 gaacccggtg taccgccagg ttctcggaat gtgtccgaca ctggccgtga ccaacggcat
    10921 gaaggccgcc atgaccatgg ccggcgcggt cgcctttgtg ctggtttgcg ccaacatcct
    10981 gaccagcctg atccgcaacc tgctcaagcc tcatctgcga attctggtgt tcacgctgac
    11041 cattgcgact ttcgtgacgg ttgccgaccg gatcatcgcg gcctatatgt acgacatgag
    11101 caagcagctg gggccctaca ttccgctgat cattgtgaac tgcatcatta tctgccgctg
    11161 tgaggtctgt gcttccaaac agaacgtgct gacagcggcc accgacgccc tggggcaatc
    11221 tctcggcttt gcgctggcac tggccagcat cgcttcggtg cgcgaaattc tcggctccgg
    11281 aagctggttc ggcatcgagg tgatgcctgc cggatggcct gactgggtgg tgatggttct
    11341 cccgccgggt gcgtttctga cactgggcct gctgctcggc gccgcaaact ggattgggga
    11401 actggctgca cgccgcagga cgacgcaggg ggtggcataa tggaatacct ttctacacta
    11461 atactgattg ccctgagcgc ggcgctgatc aacaattttg tgctgcacta tttcgttgga
    11521 atctgtccgt tcatcggcgt ctcgcgccgt gtggacatgg ccttcggcat ggggtgcgcg
    11581 gtcacgtttg tgatctccat tgcggcgctg ctgagctggc tgttgacgta ctatgtgctt
    11641 agagaggggg accgctgacg gttgtactga ccagggaccc aacggttgat ttgggcatct
    11701 tgaattacat tgtttacatc ttcgtgatc
//
LOCUS       DAOQWR010000080        11604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6012_length_11604_cov_5.301325, whole genome shotgun sequence.
ACCESSION   DAOQWR010000080 DAOQWR010000000
VERSION     DAOQWR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_11604_cov_5.301325"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKK36_07465"
     CDS             <1..844
                     /locus_tag="PKK36_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94431.1"
                     /translation="ISLGASCYLEKPFEPEDVYRVVREGVDRTRERRRRQALEEEMKG
                     IIWDLSSQSQLLIKKVEASSLAIQLMHDLQHPLGKVQRAVQAALKQCEDSAAPESVTS
                     QLTLILQEVRHCTGMVSMCGELSRGGHEPLRQISAPLMLHQIIEEVSQWASRAGIRLD
                     ARMTVNCGVLMCHPARLPAALRAVLCSSVMSVAAEKGSVRLACGDNGDALSIRIEYFD
                     GGSDQAKLGKELGDPFSPGDVEHGLGFGLAVPSRVIRDHGGDMQVRSAPGKATVIFIT
                     LPMH"
     gene            909..1802
                     /locus_tag="PKK36_07470"
     CDS             909..1802
                     /locus_tag="PKK36_07470"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNR94432.1"
                     /translation="MTLSAKQVLVLINPRSGISNPAAALLPAIEQIWHPHCPNIIHQF
                     SHSADDGRSKARNAVAAGADVVLVIGGDGMVNSIGCELIGQQAALGVIPTGSGNGFAR
                     HFNIPLDPVRAAHALAGGRTRAIDVGVANGRPFFVTCGMAWDAALVRTFEKMPVRGIL
                     PYVFSAAYEYLTYEPQPFKYSLDGGRTQRCNDPIVFTAANLTQYGGGAQIAPKACADD
                     GWLELTLMHRKDMPALLINLPRLFDGSIDRLPEVVTRRFKSLRVHRAKAAPIQLDGEL
                     VESGPDVEVDVKPCALNILIP"
     gene            1799..3202
                     /locus_tag="PKK36_07475"
     CDS             1799..3202
                     /locus_tag="PKK36_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNR94433.1"
                     /translation="MKYFLIIPMLLSFSATAAADSISGGAWTVIFNLPDQTYSTTSDP
                     EEWAIRDALLDRINALQNGHQAALATYTFSAGTTAGLILNAISAALARGASVSFVTDP
                     YVDTAALFDGTNSLAILAARPVNPLVLVQDDSSDYPVMHHKLGLFDYGGTNRQVFAAS
                     WNFTTAAGSAQWNIALLAQNPLLYSIYTNEMRELLAGRFHDHSTKSHAHDGMTFRLDP
                     GDEESWVRFAPYPESYGGNNAARDITNAIAQAQEQIVFTLNKLTLSSIAQQLIAAADR
                     GVRIHGVMPRSDTDPGEDSRSIYTNLLYAPNYATTNRVHMLTAYSKEDYSALDSGESD
                     LIHSKYMVIDPFGENPMLIHGSANWTATALVYTNGNDENVMFIRNAKAARAFYAHFKR
                     VTGAFMNEGDFWCEISSPQSVEFWTTDTNRFVLETAASPSLPWASWKTNDGGRIGMQL
                     FTNSPPDGVKIFRARRL"
     gene            complement(3199..4206)
                     /locus_tag="PKK36_07480"
     CDS             complement(3199..4206)
                     /locus_tag="PKK36_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HNR94434.1"
                     /translation="MNTHGAEARTAILKVLSEIREPAGATRIGVELGRAGINLSPRTV
                     RFHLRQLDEQGLTELISPRAGRVISEAGREELAHANIVERVGFIDARVDELAYQMDFS
                     FEKSAGTIIMNTAVILKRELSRSLAQMEPVFEKDLGMGGLLALAYPGETLGRLTIPPD
                     CIGIATVCSVTINGILVRHGIPVVSRYGGLLEIRNFKPVRFVELIEYKGTTLDPLEAF
                     IAARMTKVREAARTGEGIIGASFREVPANARAAVKKLHTRMQAASLGGIFAIGEPHRP
                     LCGIPVPDGRTGVITTAGLNPVAALQESGARIPLRSLASLEDYARFKPWRDVCRQYRR
                     G"
     gene            complement(4369..5319)
                     /locus_tag="PKK36_07485"
     CDS             complement(4369..5319)
                     /locus_tag="PKK36_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNR94435.1"
                     /translation="MIVTTKELFKHAYGKYAIGAYNINNLEQTMGLFRGNLDSKAPFI
                     IQISKGARSYTDKIMLEAIIRGADQLFPDAVFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASAEPFAKNIEITKRVVDAAHAKGIVVEAELGKLGGVEEHVSVSEADAKLTNPAEAE
                     EFVKKTGCDSLACAIGTSHGAFKFSGSQGLHFDVIAEIQKRLPGFPLVMHGSSSVPQD
                     EVARINAAGGDLKGAKGVDAGQFRKAAELGVTKINIDTDGRLVWCRVHREAFRDDPKN
                     FDLRPPGKIFMKEYAAFIAQKNEYLGSAGQLDAVRRMLKA"
     gene            complement(5388..6176)
                     /locus_tag="PKK36_07490"
     CDS             complement(5388..6176)
                     /locus_tag="PKK36_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR94436.1"
                     /translation="MSCALDNIRVVLVSPIYGGNIGAVCRCMMNCGISDLAVAAPRDD
                     VDENETVQRAYHAVEVYRKRRVFSTLEEAVADCGAIAGTSARRGLYRSHAKSPREWAP
                     GLLKSAQGGRVALVFGSEDKGLSNDHLALCTHIIQIPSHSSYVSLNLSHAVMICCYEL
                     FMESGVFDDPAEEWSPDAPSELRERMLGMWEKTLYDIGFMNDEKGDHMMMGLRRIFSR
                     GQLTVNDVKILMGIARQTQWFVNRPRALKGDGSAPPAEEVQDED"
     gene            complement(6193..6858)
                     /locus_tag="PKK36_07495"
     CDS             complement(6193..6858)
                     /locus_tag="PKK36_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR94437.1"
                     /translation="MRRLFAVFLAIASPAFAESLDVASMSFDGLMLHASRYGSTELKR
                     ERKAAARSELFARGPSALEYLIGHADSDNIWFGIYAQQMVETLPPEESAPVLLGALDS
                     KNKDIRKMAIYFLGFNRTPQYADRVLPFLECEHEAGAAMRTLGKWGVHEAVPGIIPYL
                     RDSDERRRIAAANALHDIGDPLALPALRSVRRDRYFTVRRAVARALKRLFARREVLTD
                     ESK"
     gene            complement(6855..8693)
                     /locus_tag="PKK36_07500"
     CDS             complement(6855..8693)
                     /locus_tag="PKK36_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94438.1"
                     /translation="MILPCLVSAGPEPGTNEITHLRHALSAIGMTPVDMGFMKDLGKP
                     RIALPCTRTVLSSPLKLPALADDLYRAMLEEPDNPWPLFHSWMKTKSAETTLTLDVVP
                     KFPGLNDPLQLFYAKAQESSFLLESAFGKLSSSEKEYLACTVLAGTFNAEDYESTREA
                     LTAAGFSSNLVAEVIEEGMHLDPKPNADRFLDAVEKIDFGALIEAGRIFHEAAVQLRE
                     EASAETNWPESAVVYETPLGRIAVGTESDDHYDSPYLLIVDPAGNDKYTEASAQGLKG
                     HPLSAVLDLRGDDTYAGRGMIGPGAALWGICVMVDAGGNDFYSVEYTGLGAGLFGAAW
                     LEDGSGSDIYRAHALAEGAAFAGIGVLHDLAGDDLYQAGFSSQASAGVLAIGLLMDRE
                     GNDHYLAGGREHDWERNDERFISLSQGCSIGMRPYAGGGVAALVDLAGNDVYEADVYG
                     QGVGYWYSVGMLLDAAGHDRYSLHQYGQGAGIHLSAGLLWDGEGNDVYSGYILAQGAA
                     HDYAVGMLFDESGDDTYTADHHSQGRALNTAMALLIDSAGNDAYFARQPDQCQGIGNP
                     GDKREYGSLALLLDLQGADRYSCGAEAGARLERPDFGIVYDLKEEP"
     gene            complement(8773..9660)
                     /locus_tag="PKK36_07505"
     CDS             complement(8773..9660)
                     /locus_tag="PKK36_07505"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR94439.1"
                     /translation="MRDIAHLISEKSAGLFCDYAVTQGMVCHPEKGDDGAWHIWVEDE
                     DLLEPAAQLLRDFQSHPEDPAYLAAEGAAEEFMTEAERAEKEAAKRTFDRDRLWAGRT
                     GRLTAVLMGVSILVSIVSMLGARTDLVRFLFITGVYEQGNMIQWMPGLPEIRHGQIWR
                     LITPIFLHFGIWHILFNMMWLRDLGGMIERIKGPWFLGIFIAVVASLSNIGQFYVSGP
                     LFGGMSGVVYGMLGYVWMKGRYDPGSGLFLHPTTVTMMIVWFFLCLTGLMGPVANTVH
                     GIGLGLGVAWGYISARLRL"
     gene            complement(9663..11321)
                     /gene="gpmI"
                     /locus_tag="PKK36_07510"
     CDS             complement(9663..11321)
                     /gene="gpmI"
                     /locus_tag="PKK36_07510"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR94440.1"
                     /translation="MEALKKSKKFAGPAGPVVLVIMDGVGIGKHEEGDFVRAASTPTM
                     DWLKANTVNSQLKAHGRAVGLPSDEDMGNSEVGHNAIGCGRVFDQGAQLVNNAIASRS
                     IFEGAVWKELIANCRANQSTLHFIGLFSDGNVHSHIDQLKGMLNEAAAEGIARVCLHP
                     LLDGRDVPPTSALTYIDDIEAFFGEKNAAGKTQFLIASGGGRMVITMDRYNANWSMVE
                     LGWKTHVKGEGRFFATAREAIETFRNEKPGIIDQDLPPFVIAKNGKAVGPIVDGDSVI
                     YFNFRGDRAIEMSRAFEEDNLEYFDRGSRPKVKYAGMMEYDGDLHIPKKFLVEPPAID
                     RTLDEYLIKSSVTQLAISETQKYGHVTYFFNGNRSGKFSDTLEDYIEIPSDIVPFEQR
                     PWMKGAEITDRVIAAIESGKHKFIRLNYPNGDMVGHTGIFEAVRIAVETVDLCLARLI
                     EAVKKAGGIMVISADHGNADDMYERSKKTGQPAIDPHTGGPQAKTSHSLNPVPVYIYA
                     PANKDQIKLSDAKDLGIASLAATCIKLLGFEPPADYAPSIVDAG"
BASE COUNT         2280 a         3717 c         3321 g         2286 t
ORIGIN      
        1 catttctctc ggggccagct gctatcttga aaaaccgttc gaaccggaag atgtatatcg
       61 cgtggtgcgc gagggtgttg accgcacgcg cgagcggcga agacgacagg cgctggagga
      121 ggaaatgaag gggatcattt gggatctctc cagccagtcc cagctgctga tcaagaaagt
      181 ggaggcttcc agcctcgcca tccagctcat gcatgatctc cagcatccgc tggggaaagt
      241 ccagcgggcg gtccaggccg cgctgaagca gtgcgaggac agcgcagcgc cggaatctgt
      301 gacttcccag ctcacgctga ttctgcagga agtgcggcac tgtaccggca tggtctccat
      361 gtgcggcgag ctgtccaggg gcggtcatga acccctgcgg cagatcagcg cgccgctcat
      421 gctgcatcag atcatcgaag aggtttccca gtgggcctct cgcgcaggga tccggctgga
      481 tgcccggatg acggtcaatt gcggcgtgct gatgtgccat cccgcgcggc ttcccgccgc
      541 gctgcgggcc gtgctttgca gcagcgtgat gtccgtggcc gcagaaaagg gatccgttcg
      601 cctcgcatgc ggcgacaacg gcgatgcgct cagcatccgc atcgaatact ttgatggagg
      661 ctcggatcag gcaaaactgg ggaaagagct gggagaccct ttcagcccgg gcgacgtgga
      721 gcatggactg ggttttggcc tggcggtgcc ctcccgcgtg atccgggatc acggaggcga
      781 tatgcaggtg cgaagcgcac ccggcaaggc cacagtgatc ttcatcacgc tcccgatgca
      841 ctgatttttg tctgttccgc tccggggctt cactgctatc atcactctgt aagactggag
      901 tcccgttcat gacactgtcc gctaaacagg tgctggtgct gatcaacccc aggtcgggga
      961 tcagcaaccc cgctgcagcg ctcctgccgg ccatcgagca gatatggcat ccgcactgcc
     1021 cgaacatcat ccatcagttc agccacagcg cagacgacgg acgcagcaag gcccgaaacg
     1081 ccgtggccgc cggcgccgac gtggtgctgg tgatcggtgg cgacggcatg gtcaattcca
     1141 tcggctgcga actcatcggt cagcaggcag ccctgggcgt gatccccacc ggaagcggca
     1201 acggcttcgc gaggcatttc aacattccgc tcgacccggt gcgtgcggca cacgcgcttg
     1261 caggcgggag gacccgcgcc attgatgtcg gagtcgccaa cggccggccg ttcttcgtca
     1321 cctgcggcat ggcctgggat gcggccctcg ttcgcacgtt cgaaaaaatg ccggtgcggg
     1381 ggattctccc gtatgtcttt tccgccgcct atgaatacct gacgtacgag cctcagccct
     1441 ttaagtactc gctggacgga gggagaacac agcggtgcaa cgacccgatt gttttcacgg
     1501 ccgccaacct gacccagtac ggcgggggcg cgcagattgc gccaaaggcc tgcgccgacg
     1561 acggctggct ggaactgacg ctcatgcacc gcaaggacat gcccgcgctg ctcatcaatc
     1621 tcccccgcct ctttgatggg agcatagacc gactgccgga ggtggttacc cgccgcttca
     1681 aatcactgcg ggtgcaccgc gcaaaggctg cgccaatcca gctggacggg gaacttgtcg
     1741 aatccggccc cgatgtcgaa gtggatgtga aaccctgcgc gctcaacatc ctgatcccat
     1801 gaagtacttc ctgatcatcc cgatgctgct cagcttttcc gcgacggcgg cggccgacag
     1861 catcagcggc ggcgcgtgga ccgtgatctt caacctgccg gaccagacct attcgacaac
     1921 cagcgatcct gaggaatggg ccattcgcga tgcgctgctg gaccgcatca acgccctgca
     1981 gaacggccat caggccgctc tggccaccta caccttctcg gccggcacga cggccggcct
     2041 gattctcaac gccatcagcg cggcccttgc gcgcggagct tcggtgagct tcgtcaccga
     2101 cccctatgtg gacacagccg ctctctttga cggcaccaac tcgctggcca ttctggcggc
     2161 gcggccggtc aatccgctgg tgcttgtgca ggatgattcc tcggactatc ccgtcatgca
     2221 tcacaagctc gggcttttcg actatggcgg aaccaaccgg caggtgttcg cggcctcatg
     2281 gaatttcacg acggcagccg gttcggccca gtggaacatc gcgctgctgg cgcagaatcc
     2341 cctgctctat tcgatttaca caaatgaaat gcgcgaactt cttgcgggcc ggtttcatga
     2401 tcattccacc aagtctcatg cgcatgacgg catgaccttc cggctggatc ccggcgacga
     2461 ggaaagctgg gtgcgcttcg ccccctatcc tgaatcctac gggggaaaca atgccgcccg
     2521 cgacatcacc aatgccattg cccaggctca ggagcagatc gtctttacgc tcaacaagct
     2581 cacgctgagc agcattgccc agcagctgat cgccgccgcg gaccgcggtg tccgcattca
     2641 cggcgtcatg ccgcgcagcg acaccgaccc cggcgaagac tcgcgcagca tctacaccaa
     2701 cctgctctac gcgccgaact atgccaccac caaccgggtg cacatgctca cggcctattc
     2761 aaaagaggat tactccgccc ttgactccgg agagagcgac ttgatccatt cgaagtacat
     2821 ggtcattgat ccgttcgggg aaaatccaat gctgattcac ggctcggcca actggaccgc
     2881 caccgcgctg gtctacacaa acggaaacga tgaaaacgtg atgttcatcc ggaatgcgaa
     2941 agccgcgcgg gccttttatg cccatttcaa gcgcgttacc ggcgcattca tgaacgaagg
     3001 cgatttctgg tgcgagatat cctccccgca gtccgtggaa ttctggacca ccgacaccaa
     3061 ccgctttgtg ctggaaaccg ccgcctctcc ctcgctccca tgggcctcct ggaaaacaaa
     3121 cgacggcgga cgtatcggca tgcagctctt caccaacagc ccgccggacg gtgtgaagat
     3181 tttccgcgcc aggcggctct agcccctgcg atactgacgg cagacatcgc gccacggctt
     3241 gaagcgcgcg taatcttcca gcgacgccag cgagcgcagc ggaatcctcg ccccagattc
     3301 ctgcagcgcg gccactggat tcaaccccgc cgtggtgatc actccggtcc gcccgtccgg
     3361 gaccggaatt ccgcagagcg gccggtgcgg ttcgccaatg gcaaatatgc cgcccagcga
     3421 agcggcctgc atgcgggtat gcagcttctt gaccgccgcg cgcgcgttgg cgggaacttc
     3481 ccggaaactg gcgccaatga tcccctcccc cgtccgggcc gcctcgcgca ccttggtcat
     3541 gcgcgccgcg ataaaggcct ccagcggatc cagcgtggtt cccttgtact cgatcagctc
     3601 aacaaaacgc accggcttga aattccggat ctccagcagg cctccgtacc gtgagaccac
     3661 ggggatgccg tgccgcacca gaatgccgtt gatggtcacg ctgcataccg tggcgatgcc
     3721 tatgcaatcg ggcgggatcg tcaggcggcc gagcgtctcg cccggatacg ccagggccag
     3781 caggccgccc atccccagat ccttttcaaa aaccggctcc atctgggcca gcgaccgcga
     3841 caactcccgc ttcaggatca cagccgtatt catgataatc gtgccggcgc ttttttcgaa
     3901 gctgaaatcc atctggtagg ccagttcatc cacacgtgcg tcaatgaaac ccacgcgttc
     3961 cacaatattc gcatgggcca gttcctcgcg gccggcctcg ctgatcacgc gtcccgcgcg
     4021 cgggctgatg agctccgtca gcccctgctc atcgagctgc cgcagatgaa accgcaccgt
     4081 gcgcggactc aggttgatgc cggcgcggcc gagttccacg ccgatgcggg ttgcgccggc
     4141 gggctcgcgg atttcactga gcaccttcag aatggcggtg cgggcctctg ctccatgcgt
     4201 attcatggca tgattaggca accttgccgt aattttttca agtccaaacc gacgcaggag
     4261 aaaagttcca gtccctggaa gtttttcccg ggaaagttcc agccattgga actttcaaaa
     4321 aaagagagga gcccgcaggc tcctctccgt tgaacgatct ggatgcgctt acgccttcag
     4381 catcctgcgc acggcgtcca gctgcccggc gcttcccaga tattcgtttt tctgggcgat
     4441 gaaggcggcg tattccttca tgaagatttt tcccggcgga cgaagatcga agttcttcgg
     4501 atcgtcgcgg aacgcctcgc ggtgcacgcg gcaccacacc aggcggccgt ccgtatcaat
     4561 attgatcttg gtgacgccca gttcggcggc tttcctgaac tggccggcat ccacgccctt
     4621 cgcccctttc agatcgccgc cggcggcgtt gatccgcgcg acttcatcct gcggcaccga
     4681 gctggagccg tgcatgacca gcggaaagcc cggaagacgt ttctggattt ccgcaatgac
     4741 gtcaaagtgc agcccctgcg agccgctgaa cttgaacgcc ccatggctgg tgccgatcgc
     4801 gcaggcgagg ctgtcgcatc cggttttctt cacaaactcc tccgcctcgg ccggattggt
     4861 cagcttggca tcggcttcgc tgacggatac atgctcttcc accccgccca gcttcccgag
     4921 ttcggcttcc accacaatgc ccttcgcgtg cgcggcatcc acgacgcgtt tggtgatctc
     4981 gatgtttttc gcaaacggct cggcgctggc gtcaatcatc acggagctgt aaaagccgct
     5041 gttgatgcag tcatagcagg tttcttcatc gccgtgatcc aggtgaacgg caaacaccgc
     5101 atccgggaag agctgatccg cgccgcgaat gatggcctcc agcatgattt tgtcggtgta
     5161 ggagcgggcg cccttggaaa tctggatgat gaaaggcgcc ttgctgtcga gattgccccg
     5221 gaacaagccc atggtctgct cgaggttgtt gatgttgtac gcgccgatgg catatttgcc
     5281 gtaggcatgc ttgaacagtt ctttcgttgt tacaatcaca gctctctcct ttgcgttgag
     5341 cgttttactt caatatccgc cttttttaga tggccggccg cagttagtca atcttcatcc
     5401 tgaacttctt ccgcgggcgg cgcggacccg tcgcccttca gggcccgcgg ccggttcaca
     5461 aaccactgcg tctggcgggc gatgcccatc aggattttca cgtcattgac cgtcagctgc
     5521 ccgcgagaaa aaatccggcg cagccccatc atcatgtgat ctcctttttc atcattcatg
     5581 aaaccgatgt catagagcgt cttctcccac atgcccagca tgcgctcgcg gagctctgac
     5641 ggcgcgtcgg gcgaccattc ttccgccgga tcgtcgaaca cgcccgactc catgaacagc
     5701 tcatagcagc agatcatcac cgcatgcgaa agattgagcg acacatagct gctgtgcgac
     5761 gggatctgaa tgatatgcgt gcaaagcgca aggtgatcat tggacaggcc cttgtcctcc
     5821 gacccgaaca ccagggccac ccttccgccc tgcgcgcttt tcagcaggcc cggcgcccac
     5881 tcgcgcgggc ttttggcatg gcttcgatag agtccccgtc tcgcgctcgt tccggcgatg
     5941 gctccgcagt ccgccacggc ctcctccagg gtggagaaca cccggcgctt cctgtaaacc
     6001 tccaccgcat gataggcgcg ctggacggtt tcgttttcat ccacgtcatc gcgcggggca
     6061 gccacggcca gatcgctgat gccgcaattc atcatgcagc ggcaaaccgc gccgatgttt
     6121 cccccgtaga tcggggaaac cagcaccacc cgtatattgt ccagcgcgca gctcatggga
     6181 ttgaaatgat gatcatttgg actcgtccgt caagacttca cggcgggcga acagtctttt
     6241 cagcgcgcgc gcgacagccc tgcggacagt gaaataccgg tcacgccgca ctgaacgcag
     6301 tgcggggagc gccagcgggt cgccgatgtc atgcagcgca ttggccgcgg caatgcggcg
     6361 gcgctcgtcg gaatcgcgca gatacggaat aatgcccggc accgcctcgt ggacgcccca
     6421 cttccccaat gtgcgcatgg cggcgcctgc ctcgtgctcg cactccagaa acggaagcac
     6481 acggtccgcg tactgcggcg tgcggttgaa tcccagaaag taaatcgcca ttttgcggat
     6541 atctttgttt tttgaatcca gtgcacccag cagcaccggg gcggactctt ccggcggcag
     6601 ggtttccacc atctgctggg catagatgcc gaaccagatg ttgtcgctgt ccgcatggcc
     6661 gatcagatat tccagcgccg agggcccgcg cgcaaaaagc tccgagcgcg cggccgcctt
     6721 gcgctcgcgc ttgagttccg tggacccgta tcgcgaagca tgaagcatga gtccgtcaaa
     6781 actcattgag gcaacatcga gcgattccgc aaacgccggc gaagcgatcg cgagaaaaac
     6841 ggcaaagagc cttctcatgg ctcctccttc agatcataga cgattccgaa atccggccgt
     6901 tcaaggcgcg cgccggcttc cgcaccgcag gaatagcggt ccgccccctg cagatcgagc
     6961 agcagggcaa gacttccata ctcgcgcttg tcgcccggat ttccgatgcc ctggcactgg
     7021 tccggctggc gcgcaaaata ggcgtcgttc cccgcggaat caatcagcag ggccatggcc
     7081 gtgttcagtg cccggccctg gctgtgatga tctgcggtat aggtatcatc tccggactca
     7141 tcgaaaagca tccccacggc gtaatcatgg gcggcaccct gcgcaagaat atatccgctg
     7201 tagacatcgt ttccctcccc gtcccacagc agacccgcgc tcagatgaat gcccgcgccc
     7261 tgcccgtact ggtgcaggct gtagcggtca tgaccggccg catccagaag catgccgacg
     7321 gaataccagt agccgacgcc ctgcccatag acatcggctt cgtaaacatc attccccgcc
     7381 agatccacca gcgcggcgac tccgccgccc gcataaggcc gcatgccgat ggagcacccc
     7441 tgcgaaagcg agatgaaacg ctcgtcgttc cgctcccagt catgctcgcg gccgccggcg
     7501 agatagtgat cgtttccttc acggtccatc agcagaccga tggccagcac accggccgag
     7561 gcctgcgagc tgaagcctgc ctggtacaaa tcgtcgcccg cgagatcgtg aagcactcca
     7621 attcccgcaa aggccgcgcc ctcggccagc gcgtgggcac ggtaaatatc gctgcccgag
     7681 ccgtcttcaa gccacgccgc tccgaacagt ccggcaccga gcccggtgta ttccaccgaa
     7741 tagaagtcat tgcccccggc gtctaccatc acgcaaattc cccacagcgc cgctccgggg
     7801 ccaatcattc cccggcccgc atacgtgtca tcgccccgca aatccaacac cgcgctgagc
     7861 ggatggccct tcagaccctg cgccgatgct tccgtgtatt tgtcattgcc ggccggatcc
     7921 acaatcagga gatacgggga atcataatgg tcgtcggatt cagttcccac ggcaattctg
     7981 cccaggggcg tttcataaac gacagccgat tccggccagt ttgtctcggc cgaagcctct
     8041 tcgcgcaact ggaccgccgc ttcgtgaaaa atacgcccgg cctcaatcag cgccccgaaa
     8101 tcaatttttt caaccgcatc cagaaaacgg tcggcattcg gtttcggatc aagatgcatg
     8161 ccctcttcga tcacctcggc aacaagattc gatgaaaagc ccgcggccgt gagcgcttcg
     8221 cgggtggatt cgtaatcttc ggcattgaat gttcctgcaa gaaccgtgca ggccagatat
     8281 tccttttcgg aagagctcag cttcccgaag gccgactcca gcaggaacga agattcctgt
     8341 gcttttgcat agaacagctg aagcggatca ttcaacccgg ggaatttcgg aacgacatcc
     8401 aatgtcagcg ttgtttcggc ggattttgtt ttcatccacg aatgaaacag cggccacggg
     8461 ttgtccggtt cttccagcat ggcccgataa agatcatcgg ccagggccgg cagtttcagc
     8521 ggcgacgaaa gcaccgtccg tgtgcaggga agcgcgatgc ggggcttgcc gaggtctttc
     8581 atgaacccca tgtccaccgg cgtcatgcca atggcagaca gggcgtgacg caaatgcgtg
     8641 atttcattcg ttcccggttc cggacccgca gagaccagac acgggagaat catcagcgcc
     8701 agtatgagtc ctgatcccct cttctgcata tgagaagtat gaaacatctg cggaggcaat
     8761 tcaatccaat cattacaggc gcaggcgggc ggagatatag ccccaggcca ctcccagccc
     8821 gaggcctatt ccatgaacgg tgttggcgac cggacccatc aggccggtga gacagagaaa
     8881 gaaccagacg atcatcatgg ttaccgttgt cggatgcaga aacagtccgg agccgggatc
     8941 atatcggccc ttcatccaga catatcccag cattccatac accacgccgg acatcccgcc
     9001 gaacagcggg ccggacacgt agaactggcc gatattggag agcgatgcaa caacggcgat
     9061 gaaaatccca aggaaccacg ggcccttgat gcgctcgatc attcctccga gatcgcggag
     9121 ccacatcatg ttgaagagga tatgccagat gccgaagtgg aggaagatcg gcgtaatgag
     9181 ccgccagatc tggccgtggc ggatttcagg caggcccggc atccactgga tcatattgcc
     9241 ctgctcataa accccggtga tgaacagaaa ccggaccaga tcagtgcgcg cgccaagcat
     9301 ggaaactatg ctgaccagaa tggaaacgcc catcagtaca gccgtcagac ggcccgtgcg
     9361 tccggcccac aaccggtcac gatcaaaggt gcgctttgcg gcctccttct cggctctctc
     9421 cgcttcggtc atgaattctt cggcggcgcc ttccgcagcc agatacgcgg ggtcttccgg
     9481 atggctctga aaatcacgga gcagctgcgc ggcgggttcc aggaggtcct cgtcctcgac
     9541 ccagatgtgc catgccccgt catcgccctt ctcggggtga cagaccatgc cctgcgtcac
     9601 cgcatagtcg cagaaaaggc ccgcgctttt ttcagagatc agatgtgcga tatcgcgcat
     9661 tttcagccgg catccacaat gctgggcgcg taatccgccg gcggctcgaa gcccagcagc
     9721 tttatgcagg tcgcggcaag gctggcgatt cccaggtctt tggcgtcaga cagcttgatc
     9781 tgatccttgt ttgcaggagc atagatataa accgggacgg gattgagcga atgactggtc
     9841 ttggcctgcg ggccgccggt gtgcggatca atcgccggct ggccggtttt cttgctgcgc
     9901 tcgtacatgt cgtccgcgtt gccgtgatca gccgaaatca ccatgatgcc gccggccttc
     9961 ttcaccgcct caatcagccg cgcaaggcag aggtccacgg tttccaccgc tatgcggaca
    10021 gcctcgaata ttcccgtgtg gccgaccatg tcgccgttgg gatagttcag gcggatgaac
    10081 ttgtgtttcc cgctttcgat ggccgcgatc acgcggtcgg tgatttccgc gcccttcatc
    10141 cacggccgct gctcaaaggg cacaatgtca gacggaatct caatataatc ctccagcgta
    10201 tcgctgaatt tcccggagcg gttgccgttg aagaaatagg tcacgtgccc gtatttctgc
    10261 gtctcgctga tggcgagctg cgtgacgctg gacttgatca ggtattcatc cagtgtgcgg
    10321 tcaatggccg gcggctccac caggaatttc ttcgggatgt gcaggtcgcc gtcatattcc
    10381 atcatgcccg catacttcac tttcggccgc gagccgcggt cgaagtattc cagattgtcc
    10441 tcctcaaacg cgcgcgacat ttcaatggcg cggtcgccgc ggaagttgaa atagatcacg
    10501 ctgtcgccgt caacgatcgg tccgacggcc tttccgttct tcgcgatgac gaacggaggc
    10561 agatcctggt caatgatgcc gggcttttca ttccggaatg tttcgatcgc ctcgcgcgcg
    10621 gttgcgaaaa agcgcccctc gcccttcacg tgggtcttcc agcccagctc caccatgctc
    10681 cagttggcgt tgtaacggtc catcgtgatg accatgcggc cgccgccgga cgcgatcaga
    10741 aactgcgtct tgccggctgc gttcttttcc ccgaagaagg cttcaatgtc gtcaatgtaa
    10801 gtcagggcgg aagtcggcgg aacatcgcgg ccgtcgagaa gcgggtgcag gcatacgcgc
    10861 gcgatgccct cggcggcggc ctcgttcagc atgcccttga gctggtcaat atggctgtgg
    10921 acgttgccgt cggaaaacag gccgatgaaa tgaagggtgg actggttcgc cctgcagttg
    10981 gcgatcaatt ctttccagac cgcgccctcg aaaatgcttc gcgaggcgat ggcattgttc
    11041 acaagctgcg cgccctgatc aaaaacgcgg ccgcagccga tggcgttgtg gccaacttcg
    11101 ctgttgccca tgtcctcgtc cgacggcagg ccgacggcgc ggccgtgggc cttgagttga
    11161 gaattgaccg tattggcctt cagccagtcc atggtcggcg tggaggcagc gcggacgaaa
    11221 tcgccctctt cgtgttttcc gatgccgacc ccgtccatga tcaccagcac taccgggccg
    11281 gccgggccgg cgaacttttt cgatttcttc aacgcttcca ttttccgcct cgtcgttctt
    11341 ttgttggatt gaatgaacaa tatgcaccgt gatgcgccaa agcgcaagtt tgatgaggaa
    11401 acgggcccgg tcaaggagta acctctctgc cggctggtgt gacctttcgc tccggcgggg
    11461 caagcccacc gggggcggga agcatccgga cccccgcgac cctgcgctcc ggcgaaacaa
    11521 gcccaccggg gggcgggaag catccggacc ccccgcgacc ctgcgctccg gcggggcaag
    11581 cccaccgggg gcgggaagca tccg
//
LOCUS       DAOQWR010000081        11320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6187_length_11320_cov_5.117177, whole genome shotgun sequence.
ACCESSION   DAOQWR010000081 DAOQWR010000000
VERSION     DAOQWR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6187_length_11320_cov_5.117177"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..231
                     /locus_tag="PKK36_07515"
     tRNA            155..231
                     /locus_tag="PKK36_07515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:189..191,aa:Ile,seq:cat)
     gene            complement(247..2289)
                     /locus_tag="PKK36_07520"
     CDS             complement(247..2289)
                     /locus_tag="PKK36_07520"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNR94441.1"
                     /translation="MRTELLIAAFGAGLAALVFALWKTAWINRQSPGTERMQEIGSAV
                     REGAMAFLNREYKVLAVFVLAVALLMLFAYEGAQKLVALSFVVGAVCSSLAGWFGMRV
                     ATAANMRTTEGARHGLQPALRVAFSGGTVMGMSVVGLAMLGLSGLFIVYTRKFGTELS
                     ALKGTILPVITGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPA
                     TIADNVGDNVGDVAGMGADLFESYVGAIVGAMVLGAAAGQVKLVTLPLVVAGIGIIVS
                     IAGTFFVRTKEGGNPQTALNMGTFGAAVIMALLTWPIVMKFAPEQFVLGETTYSAAGI
                     FWATIAGLVAGVAIGMLTEYYTAENKRPAQKIAEASETGSATNIIAGLGTGMLSTALP
                     ILCIGVAIVLSFRFAGLYGIAIAALGMLATTGIQLAVDAYGPIADNAGGLAEMAKLEP
                     EVRKRTDKLDAVGNTTAAIGKGFAIGSAALTALALFAAFMETAGLKVIDVSKPMVMAG
                     LFLGGMLPFLFSSLAMGAVGRAAYAMIEEVRRQFREIPGLLDGNAKAEYAKCVDISTK
                     AAIREMVLPGLLAVLTPVLVGFVFGPEMLGGVLAGVTVSGVMLALFMSNAGGAWDNAK
                     KHIEAGNFGGKGSEAHKAAVTGDTVGDPFKDTAGPALNILIKLMSVVSLVIAALLV"
     gene            complement(2379..2993)
                     /locus_tag="PKK36_07525"
     CDS             complement(2379..2993)
                     /locus_tag="PKK36_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94442.1"
                     /translation="MKRGRGRNGRRTFLVLAVVALPVLRAYSQPANAVPADSWAETAY
                     IAMNKGNTAEAISLFKDALKADPKSRNAWFGLATVHIQIRDYATAEKILLKLAQAYPE
                     DYAIRNNIAWMYATAENPKFRNAEKAVKWARDAVFLSPLSPELWSTLSEAHYLAGDYE
                     KAFRAVEHVMDIAPREKLSREQARIYAEQYSKCKKAKEAMSLIP"
     gene            complement(2990..3931)
                     /locus_tag="PKK36_07530"
     CDS             complement(2990..3931)
                     /locus_tag="PKK36_07530"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNR94443.1"
                     /translation="MPFESRIPEWLRSNFAAKLGAVLLAVGTWFIIDSIISNKELLSD
                     VPLVIRTGEGLAVLKRSVDSVDVVFRGSREDIQRLKRGEVSVEISTGEASGRTVIRLK
                     PEHVKAPRGCRVVSMDPETVDVELDREDEKKVPVRPVTEDALPDGYVLDKLIPTPTNV
                     TIRGPVGQLKDVKEIRTRPISLKGRNGTFRERIALAAPSDIWDARMDPERVMVEAAIV
                     EHTSEKLVEAVPVSAMVGTGSRLLIRVEPSHVNITLKGGEDILKEADVTRISAYVDCS
                     GMDAGASSVLPVKVVPLPRIAVDRVEPDVVNVEVGVR"
     gene            complement(3921..4781)
                     /gene="cdaA"
                     /locus_tag="PKK36_07535"
     CDS             complement(3921..4781)
                     /gene="cdaA"
                     /locus_tag="PKK36_07535"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNR94444.1"
                     /translation="MPEIIAQIITPMLEMLVLAVMYYYVFLFLRGTRGWQMLVAFVVA
                     LLVLQFVTEFFHLTTLNWVLAKFTAYIAIAFIVIFQPEIRRALAELGKQHVFAGTQRE
                     RGLCDHLVQATLMLAERKIGALIAVEQEIGMRTIQETGTPLDARVTPELLSTIFYPGT
                     PLHDGGVLVRENRVVAAGCLFPLSQRVELGRSLGTRHRAAIGLTEETDAVVLVVSEET
                     GTISIAYRGRLLRPLDEDKLRRILTRIMLRSRKGKSRINRVQELLDLSPDGVAKTEVI
                     SSEQGGADAV"
     gene            complement(4766..5641)
                     /gene="folP"
                     /locus_tag="PKK36_07540"
     CDS             complement(4766..5641)
                     /gene="folP"
                     /locus_tag="PKK36_07540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNR94445.1"
                     /translation="MRRTCQWKCRDRTLACGSRTLIMGILNVTPDSFSDGGLYRFAPD
                     AAAHALDMEADGAAIIDIGGESTRPGAAEVPADEELGRVIPVIRMLAGKTRCALSIDT
                     TKARVARAALEAGCHIVNDISAGAADPEMLSVVRESGAGYILMHMQGTPRTMQQNPVY
                     ENVLEEVCRLLSDRSEACLAAGVAHEALALDPGIGFGKTVDHNLDLLAGLDRLAALGF
                     PVVAGLSRKSFLGVLTGAGPSGRLAGGLAAMAFAAAHGAAVLRVHDVKESCDAARVVD
                     ILLAHEQDMKECRKS"
     gene            complement(5655..7601)
                     /gene="ftsH"
                     /locus_tag="PKK36_07545"
     CDS             complement(5655..7601)
                     /gene="ftsH"
                     /locus_tag="PKK36_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNR94446.1"
                     /translation="MEEHKDAPQKEAGPPQQKMPFRGMAVWFLLIALFLTLFQVFSQR
                     QVKYEEIAWSPAFVDLVKNNRIRDCEIVRDLAGNNEYIRGKKLDGSKVTHFRVNTIVT
                     EYTQKWLDENGVSYQYKSQNPYLWQIISSAIPLLLFIGLLYFFFIRQMKMAGRGAMSF
                     GKSRARMLTRDRNLITFKDVAGIDEAEEEVQEIIEFLKDPKRFTKLGGRIPKGVMLVG
                     APGTGKTLLAKAIAGEAKVPFFSISGSDFVEMFVGVGASRVRDMFQQGKKHAPCIIFI
                     DEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFETQEGVIIVAATNRPDVLDNALL
                     RPGRFDRQIVVDLPDQTGRENILKIHSKKVKLGATADLRRVARGTPGFSGADLANLIN
                     ESALLAARRGASAIEMKDMEEARDKVRWGRERRSRRLDEKERRLTAYHEAGHAIVLAM
                     MKETEPLHKVTIIPRGMALGATMQLPEKDRYTQTRQSLLDMIAGMMGGRSAEEIVFND
                     ITTGAQSDIKQSTHIARMMVCNWGMSDVLGPQSFGKGEELLFLGREVARSQDHSEETS
                     RKIDAEVSRIINEGHERALTVLRDHREHLDKLAGILLERETVEGFEVEEILKHGRILD
                     EAERAEIRNRENPAVPADEPPAAG"
     gene            complement(7699..9084)
                     /gene="tilS"
                     /locus_tag="PKK36_07550"
     CDS             complement(7699..9084)
                     /gene="tilS"
                     /locus_tag="PKK36_07550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNR94447.1"
                     /translation="MNLPGRVLDFILRGGLLRGGEHVLAAVSGGADSVAMLHLLSDLA
                     PQLGIRLSVAHLNHRLRGREAARDARFVEQLAKSLGMPFMGSSCEVRKLARERNVSVE
                     MAARMARYKFLAEAREKLGADCVAVAHNSDDQVETVLLKLARGSVAGLGGMKAVTAWG
                     GLRVIRPLLGTPRPEIMRYLKGRRLEWVEDSSNAGRKHLRNRVRHEVIPLIEKELNPQ
                     FRKAVLQTAGIVAEDQEWLDELSVALLKKCSVGEGLSMQKLNRLHPAARRRVLMLWLQ
                     PCGDVTFELLKRIEVVLAGEKGSQWVPGPGGLRVGRRYDLLVLETAREGKGFNRPVKI
                     PGATALKKQYLVVTAERCRTLSRQAGGKIGEYPAWACVNASVLKGRGLRVRSWRAGDR
                     FAPLGIAGTKKIQDIFVDAKIPQALRGDIPLFAAGDEIIWIPGYRVARSAAVTDPSKG
                     VILLRVERGNS"
     gene            complement(9081..10355)
                     /gene="serS"
                     /locus_tag="PKK36_07555"
     CDS             complement(9081..10355)
                     /gene="serS"
                     /locus_tag="PKK36_07555"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR94448.1"
                     /translation="MLDIKKLRENPDEISRRLALRGKTYDLQAVLALDQDRRRLQTET
                     EKLKSERNSASKEIGRMKKAGESTDAMQESVRKTGERIAEIDAELKGLEQRQHEAMLM
                     IPNLPHASVPAGASEADNQLVRTWGSPREFSFTPAPHWDIGARLRILDFERAARMSGA
                     GFPLFRGAGARLERALIQFMLDLHTTQHGYTEIAPPFLCGTDAMTGTGQLPKMAEDMY
                     QLAGEQLYLIPTAEVPVTNIYREEIISEPLPLSFVAYTPCFRREAGAAGKLTRGLNRL
                     HQFDKVELVKIVEPAKSYEAHEQIVSHAEAVLQKLGLHYRVLELCTADISFAAAKCYD
                     LELWAPGQNAWLEVSSCSNFEDFQARRAGIRYRNAEGRVEYVHTLNGSGVALARLYVA
                     ILENYQEADGSVVIPEVLRPYMGGLERIQPAS"
     gene            complement(10377..11114)
                     /locus_tag="PKK36_07560"
     CDS             complement(10377..11114)
                     /locus_tag="PKK36_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94449.1"
                     /translation="MRLFLSFFVALCMSGLVQAQEPVEAVSGESAPAVVEAAAAAVEA
                     AVEESSPEAAGTIEIPAQEAAPEPEPCAAPVNGVALVTAGEVEADLAARVQAYVQANL
                     FTTVMLLEPMELAEGALDGQAQAAVARKKENHLAVLVLASLEGNPHHGASYPEQSVAV
                     INVAALRPDPMDAELYGRRVEKQLMSAGGILLGMEICPNPLCALSAYMDMERLDAKGR
                     NYCPPCQDRVRKILEAKNALLEPGAAQ"
BASE COUNT         2198 a         3739 c         3227 g         2156 t
ORIGIN      
        1 gtgcccccgg ccacgccgaa caccacaggc tgaatccgaa ggctgttcat ttgaagccat
       61 tctgcactgt caaaggtcca gcttcaagga aatcccgaat caatccgggg gttgcaattc
      121 gcccggttat tgggtaggtt gcttcatctc aaacgggccc ttagctcagt tggtcagagc
      181 ggaagactca taatcttttg gtcgcaggtt cgagtcctgc agggcccacc agttcccctt
      241 cggcggctag accagcagcg cggcaatcac cagagacacc acgctcatga gcttgatgag
      301 gatattcagc gcgggcccgg cggtgtcctt gaacgggtcg cccaccgtgt cgcccgtaac
      361 cgccgccttg tgtgcttctg agcccttgcc gccaaagttt ccagcctcaa tatgtttttt
      421 ggcgttgtcc caggcgccgc cggcgtttga cataaacagc gccagcatca cccccgacac
      481 ggttacgccg gccagtacgc cgcccagcat ctcggggccg aatacaaacc cgaccagcac
      541 cggggtcagc acggccagca ggccgggcag caccatttcc ctgattgcgg ccttggtgga
      601 tatgtccacg cacttggcat attccgcttt tgcattgccg tcaagaaggc ccgggatctc
      661 acgaaactgg cgccgaacct cttcaatcat ggcataggct gcgcggccca cagcgcccat
      721 cgcgagggaa gaaaacagga acgggagcat gccgcccaga aacaggccgg ccatgaccat
      781 cggcttgctg acatcaatga ctttcagccc ggccgtttcc atgaaagcgg caaaaagcgc
      841 gagggccgtc agcgcggcgg aaccaatggc aaaacccttg ccaatggccg cggtcgtgtt
      901 cccgacggca tcaagcttgt ccgttctctt ccggacttcc ggctccagtt tggccatttc
      961 cgccagaccg cccgcattgt cggcaatcgg gccataagca tccaccgcga gctgaatgcc
     1021 ggtggtcgcc agcatgccga gagcggcaat agcgattcca tagagccccg caaaccggaa
     1081 agaaagcaca atggccacgc caatgcacag aatgggcagg gccgtggaaa gcattcctgt
     1141 gcccagtccc gcaatgatat tggtggcgga tcccgtttcg gaggcttcgg cgattttctg
     1201 cgccgggcgc ttgttctccg ccgtgtagta ctccgtgagc atgccgatag ccacacccgc
     1261 cacaagaccc gcgatggtgg cccagaaaat cccggccgcg ctgtatgtcg tttcgcccag
     1321 tacaaactgt tccggcgcga atttcatgac gatgggccag gtaagcagcg ccatgatgac
     1381 ggcggcgccg aaggtcccca tgttcagggc cgtctgcgga ttgccgccct cctttgtgcg
     1441 gacgaagaaa gtgcctgcga tggatacaat gatcccgatc cccgcgacaa ccagcgggag
     1501 cgtcacaagt ttgacctgtc cggcggccgc gcccagaacc atggccccca caatcgcgcc
     1561 cacataggat tcaaaaagat cggcgcccat gccggccacg tcgcccacgt tatctccgac
     1621 attgtcggcg atggtggccg ggttgcgggg atcgtcttcc ggaattcccg cctccacctt
     1681 gcccacaagg tccgccccca catccgccgc cttggtatag atgcctccgc ccacgcgggc
     1741 gaacaaggca atggagctgg cgccgaagga gaaacccgtg atcacaggaa gaatggttcc
     1801 cttgagagca gaaagctccg tcccgaattt tctggtatag acaatgaaaa ggcccgaaag
     1861 gcccagcatg gccagtccca ccacgctcat gcccatgact gttccgccgg agaaggccac
     1921 ccgcagggcg ggctgcaggc cgtgccgcgc gccttccgtg gtgcgcatgt tggccgcagt
     1981 ggcaacccgc atgccgaacc agccggccag cgaagagcat accgcgccca cgacaaacga
     2041 cagagccaca agtttctggg cgccttcata ggcaaaaagc atcagaagcg ccacggccag
     2101 gacaaagaca gccaggactt tgtattcacg gttaagaaag gccatggcac cctcgcgtac
     2161 ggcagatccg atttcctgca tgcgttctgt cccgggggac tgcctgttga tccaagcggt
     2221 cttccacagc gcgaaaacaa gcgctgcaag ccccgcaccg aatgccgcaa tcaatagttc
     2281 tgttctcatg cctgtctttt cctccgtatt aaatccgtac tctagatgca cggcgcaaaa
     2341 cagtcaatct ttctgaaatg aatccgtgct cttcccgtct agggtatgag agacatagcc
     2401 tccttcgcct tcttgcattt cgagtattgt tccgcataaa tacgcgcctg ttcacgggaa
     2461 agcttctcgc gcggcgcgat atccataaca tgttcaaccg ccctgaaggc cttctcgtaa
     2521 tcacccgcca gataatgcgc ctcggaaagc gtgctccaca gctccggact cagaggggaa
     2581 aggaaaacag catcacgcgc ccatttcacg gccttctccg cattccggaa cttcggattc
     2641 tcggcggtcg catacatcca tgcgatattg ttcctgatgg cataatcctc tggataagcc
     2701 tgcgccagct tcagcagaat tttctccgcc gtggcgtaat ccctgatctg gatatgcacc
     2761 gtggccaggc caaaccatgc gttgcgggat tttggatcgg ctttcagcgc atccttgaac
     2821 agggatatgg cttcggccgt gttgcccttg ttcatcgcga tataggcggt ttccgcccag
     2881 ctgtccgcgg gcacggcatt ggcgggctga gaatatgccc tcaatacggg cagcgccacg
     2941 acagccagaa ccagaaaggt gcgccggccg ttcctgcccc gccccctctt caccggacac
     3001 caacctccac attgacgaca tccggctcga cgcggtccac ggcgatgcgc ggcagcggaa
     3061 caaccttgac cgggagcacg gaagaggctc cggcatccat gccggaacag tccacatagg
     3121 cgctgatgcg ggtgacatca gcctccttca gaatatcttc accgcctttc agcgtgatat
     3181 tcacatgcga tggctcaacg cggattaaaa gccgggaacc tgttcccacc atggccgaaa
     3241 cagggacagc ctccacgagc ttttccgagg tgtgctcgac aatagccgcc tccaccatca
     3301 cccgctcggg atccatgcgc gcatcccaga tgtccgatgg agcagcaagt gcaatacgct
     3361 cgcgaaaagt gccgttgcgc cccttgagcg atatgggacg cgtcctgatt tccttcacat
     3421 ccttaagctg tccaacaggc ccgcggattg tgacgttggt gggtgtcggg atgagtttat
     3481 caagcacata gccgtccggc agcgcatctt ccgtcaccgg ccgcacaggc accttcttct
     3541 catcttcgcg atcgagttcc acatccaccg tctcggggtc catcgagacc acacggcagc
     3601 cccggggcgc cttcacatgc tccggcttca ggcgaatgac ggtcctgccg gaggcctcgc
     3661 cagttgaaat ttccacagaa acctctcccc gcttcaggcg ctgtatatct tcgcgcgatc
     3721 cgcggaacac gacgtccacc gagtccacgg aacgcttcag cacggcgagc ccctccccgg
     3781 tgcgaatcac cagcggaaca tcggaaagca gttccttgtt gctgatgatg ctgtcaatga
     3841 tgaaccatgt gccaaccgcc agcagcaccg cgcccagctt ggctgcgaaa ttgctgcgca
     3901 gccattcggg aattctcgat tcaaacggca tccgcccctc cctgctccga tgagatcacc
     3961 tcggttttgg ccacgccgtc cgggctgaga tccagcagtt cctgcacacg gttgatccgg
     4021 ctcttcccct tgcggctgcg cagcatgatg cgcgtcagga tccggcgcag cttgtcttcg
     4081 tcaagcgggc gcaggaggcg cccgcgatag gcgatggaga tggtgccggt ttcttctgaa
     4141 accacgagca caacggcatc agtctcctcc gtcagtccga tggcggcacg gtgccgcgtg
     4201 cccagagaac gtcccagctc gacacgctgc gaaaggggga agaggcagcc ggccgccaca
     4261 acgcggtttt cgcggaccag caccccgccg tcatgcagcg gcgtgcccgg atagaaaatg
     4321 gtggagagca gttcgggcgt gacgcgggcg tcaagggggg tgcccgtttc ctgaatggtc
     4381 cgcatgccga tttcctgctc cacggctatc agcgcgccga ttttccgttc ggccagcatc
     4441 agggtggcct gcaccagatg atcgcacagg cctctctcgc gctgtgtgcc cgcgaacaca
     4501 tgctgtttgc ccagttcggc aagcgcacgc cggatttcag gctggaagat cacaatgaac
     4561 gcgatggcta tataggccgt gaacttcgct agcacccagt tcagggtggt caaatggaag
     4621 aactccgtca caaactgcag gaccagcaga gcgaccacaa aagcgaccag catctgccag
     4681 ccgcgtgtgc cccgcagaaa gaggaacaca tagtaataca tcacagcgag cacgagcatt
     4741 tccagcatcg gcgtgataat ctgggctatg atttccggca ttctttcata tcctgttcat
     4801 gcgcgagcag tatatccaca acgcgggcgg catcgcaaga ctcctttaca tcatgcacgc
     4861 gtaaaaccgc ggcgccatga gccgccgcaa aggccatcgc cgcaagccct cccgccagcc
     4921 ggcccgacgg accggcgccg gtcagcaccc cgagaaaact cttccgcgac aggcccgcca
     4981 cgacagggaa tccgagcgcg gccaggcgat ccagcccggc cagaaggtcg agattgtggt
     5041 ccacggtttt cccgaatccg atgcccggat ccagcgccag cgcctcatgc gccacgccgg
     5101 ccgccaggca ggcctccgac cggtcggaaa gcaggcggca gacctcttcc agcacattct
     5161 cataaaccgg attctgctgc atggtgcgcg gagtgccctg catgtgcatc agaatatagc
     5221 cggcgccgga ctcccgaacc acggagagca tctccggatc agcggcgccc gcggaaatgt
     5281 cgttcacgat gtggcatccg gcttccagcg ccgcccgggc cacccgggct ttggtggtgt
     5341 caatcgaaag cgcgcagcgg gttttccccg ccagcatgcg gatgaccgga atgacccgcc
     5401 ccagctcttc atccgcaggc acttctgccg cccccggacg cgtggactct ccgccaatat
     5461 cgattatggc ggcgccatcc gcctccatgt ccagcgcatg cgccgcggca tcgggcgcga
     5521 aacgatacag gcccccgtcg gaaaacgagt cgggcgtcac gttaaggatg cccatgatca
     5581 gtgtgcgcga gccgcaggcg agggttcgat cacggcattt ccactggcag gttctgcgca
     5641 tgcgcggcgg actcctatcc cgcggcgggc ggctcatccg caggcacggc cggattctcg
     5701 cgattccgga tttccgcccg ctccgcttcg tccaggatgc gcccgtgctt aaggatttcc
     5761 tccacttcaa atccttcgac ggtttcgcgc tccagcagaa tgccggccag tttgtccaga
     5821 tgctcgcggt gatcgcgcag caccgtcagc gcacgctcat gtccctcatt gatgatgcgg
     5881 ctgacttcgg cgtcaatttt ccgggaggtt tcctcgctgt gatcctgcga acgggccact
     5941 tcgcggccga gaaaaagaag ttcctctccc ttgccgaaac tctgcgggcc cagcacgtcg
     6001 ctcatgcccc agttgcacac catcatgcgg gcgatgtggg tggactgctt gatgtcgctc
     6061 tgcgcgcccg tggtgatgtc gttgaagacg atttcttcgg ccgagcgccc gcccatcatg
     6121 ccggcaatca tgtccagcag gctctgacgg gtctgggtgt agcggtcttt ctcgggcagc
     6181 tgcatcgtgg cgcccagggc catgccgcgc gggataatgg tcaccttgtg cagcggctct
     6241 gtctccttca tcatcgccag cacaatggcg tgaccggcct cgtgataggc cgtcaggcgg
     6301 cgctctttct cgtcgagccg gcgggaccgc ctttcgcgtc cccagcggac cttgtcgcgc
     6361 gcctcttcca tatccttcat ttcaatggcc gatgcgccgc gccgcgccgc cagcagagcg
     6421 ctctcgttga tcaggttggc cagatccgcg cccgaaaaac ccggagtgcc ccgcgccacc
     6481 cggcgcaggt cggcggtggc gcccagcttc acttttttgg aatgaatctt taaaatgttc
     6541 tcgcggccgg tctggtccgg gaggtccacc acgatctgcc ggtcgaaacg gccgggccgc
     6601 agcagggcgt tgtccagcac atccgggcgg ttcgtcgcgg ccacgatgat cacgccctcc
     6661 tgggtctcaa aaccgtccat ctccaccagc agcgcgttca gcgtctgctc gcgttcatca
     6721 tgcccgcccc cgataccgct gaagcggctg cggccgaccg cgtcaatttc atcaatgaag
     6781 atgatgcacg gcgcgtgctt cttcccctgc tggaacatgt cgcgaacgcg cgatgcgccc
     6841 acgcccacga acatttccac aaagtccgag ccgctgatgc tgaagaacgg caccttggcc
     6901 tcgccggcga tggccttggc gaggagcgtc ttgccggtgc cgggggcccc gaccagcatc
     6961 acgcctttcg ggatgcggcc gcccagcttg gtgaagcgct tgggatcctt caggaactcg
     7021 atgatctcct gcacctcctc ctcggcctcg tcaatgcccg cgacatcctt gaaggtgatc
     7081 aggttgcggt cgcgggtcag catgcgcgcg cggctcttgc cgaaactcat ggcgccgcgg
     7141 ccggccattt tcatctgccg gatgaagaag aagtacagca ggccgatgaa aagcagcagg
     7201 ggaatcgcac tggagatgat ctgccacaga taggggttct gcgatttgta ctgatagctg
     7261 accccgttct catccagcca tttctgcgtg tactccgtga caatggtgtt cacgcgaaaa
     7321 tgcgtgacct tgcttccatc cagcttcttc ccgcggatat attcattgtt tcccgccaga
     7381 tcgcgcacaa tttcgcagtc gcgaatccgg ttgttcttga ccaggtccac aaacgcggga
     7441 ctccatgcga tttcctcgta tttgacctga cgctgcgaga acacctggaa aagcgtcagg
     7501 aaaagggcaa tcagcaggaa ccagaccgcc atcccgcgga acggcatctt ctgctgcgga
     7561 ggaccggctt ctttctgcgg agcatcttta tgttcttcca tctttctcca tcccaagttg
     7621 caatgaatgc cgaatctaac accgcccatc caactttgca acggatatga ggcggccgcg
     7681 gcgattaaga agagcgcgtc aggagtttcc gcgttcgacg cgcagaagaa tgaccccttt
     7741 cgacgggtcg gtcacggccg cgctgcgcgc aacgcgatag cccggaatcc agatgatttc
     7801 gtcgcccgcc gcgaacagcg ggatatcccc gcgcagggcc tgcggaatct tggcgtccac
     7861 aaagatgtcc tgaatctttt tcgtgcccgc aatgcccagc ggcgcgaacc ggtcccctgc
     7921 ccgccacgac cgcacacgca gtccgcgccc cttcagaaca gaggcattga cgcacgccca
     7981 tgccggatat tccccgatct tcccgccggc ctgccggctg agcgtccggc acctttcggc
     8041 cgtcacgacc agatactgct tcttcagcgc cgtcgcaccc gggatcttca ccgggcggtt
     8101 gaatcctttt ccctcccggg cggtttcaag gacaagaaga tcgtagcgcc ggccgacgcg
     8161 caaaccgccc ggccccggga cccactggct ccccttttcg ccggccagca cgacttcgat
     8221 tcgcttcagc agttcaaacg taacatctcc gcagggctga agccacagca tcagcacgcg
     8281 gcggcgggcg gcgggatgca gccggttgag cttctgcatg gaaaggccct cgccgaccga
     8341 acattttttc agcagggcca ccgagagttc atccagccac tcctgatcct cagcgacgat
     8401 cccggccgtc tggagcacgg ctttgcggaa ctgcggatta agctcctttt cgatcagcgg
     8461 gatgacctcg tgccggacgc ggttgcgcaa atgcttccgg ccggcgttgc tggagtcctc
     8521 aacccattcc agccggcggc ccttcaggta gcgcatgatt tccggacgcg gagtgcccag
     8581 cagcggacgg atcacccgca ggccgcccca ggcagtgacg gccttcatgc cgcccagccc
     8641 ggccacgctt ccgcgggcga gcttgagcag gaccgtctca acctgatcat cggagttatg
     8701 ggccaccgcc acgcaatccg cgccaagctt ttcccgggct tcggccagga atttgtaacg
     8761 ggccatgcgg gcggccattt ccacggaaac gttccgctcg cgcgcgagtt tcctgacctc
     8821 gcaggatgaa cccatgaaag gcatgcccaa acttttcgcg agctgttcaa caaaccgcgc
     8881 gtcgcgcgcg gcctcacgcc cccgcagccg gtgattcaaa tgcgcgacgg aaagccggat
     8941 gccgagctgc ggggcgagat cgctgagcaa atggagcatg gccactgagt ccgcgccgcc
     9001 ggagaccgcg gccagcacat gctcgccgcc cctgagcaga ccgcccctca gaatgaagtc
     9061 cagaaccctg ccgggcaggt tcatgacgcg ggctgaatcc gctccaggcc gcccatgtac
     9121 ggccggagca cttccgggat caccaccgag ccgtcggcct cctggtagtt ttccaggatg
     9181 gccacataga gccgcgccag cgcgacgccc gagccgttca gggtgtgcac atattccacc
     9241 cggccctcgg cattgcgata gcggatgccg gcgcggcgcg cctggaagtc ctcgaagttg
     9301 ctgcaggagg aaacttccag ccatgcgttc tgcccgggcg cccagagctc caggtcatag
     9361 catttggccg cggcgaagct gatgtccgcg gtgcagagtt ccagcacgcg gtaatgcagt
     9421 cccagtttct gcaggacggc ctcggcatga gacacgatct gctcgtgggc ctcgtacgat
     9481 ttcgcgggct caacgatctt gaccagctca accttgtcga actgatgcag gcggttcagg
     9541 ccgcgcgtca gcttgcccgc cgcgccggcc tcgcgccgga aacagggcgt ataggccacg
     9601 aatgacaggg gcagcggctc gctgatgatt tcctcgcgat agatgttcgt gaccggcact
     9661 tcggccgttg gaatcagata gagctgttcg cccgcaagct gatacatgtc ctccgccatt
     9721 ttcgggagct gtccggtgcc ggtcatggcg tcggtgccgc acaggaaggg cggcgcaatt
     9781 tcggtgtagc cgtgctgtgt cgtgtgcaga tccagcatga actgaatcag cgcccgctcg
     9841 agccgcgcgc ccgcgccccg gaacagcggg aagcccgcgc ccgacatccg cgcggcgcgc
     9901 tcaaaatcca gaatcctgag ccgtgcgccg atatcccagt gcggcgcggg cgtgaagctg
     9961 aactcgcgcg gggagcccca tgtgcgcacg agctggttgt ccgcctcgct ggcgccggcg
    10021 ggaacgcttg cgtgcggcag attcgggatc atgagcatcg cctcatgctg acgctgctcc
    10081 agccctttaa gctccgcatc aatttccgca atgcgctcgc cggttttgcg aacggactcc
    10141 tgcatggcat ccgtgctctc gcccgccttt ttcatgcggc cgatttcctt cgaggcggaa
    10201 ttgcgctcgc tcttcagctt ttcggtttca gtctgcaggc ggcggcggtc ctgatccagg
    10261 gccagcacgg cctgcagatc gtacgtcttg ccgcgcaaag cgaggcggcg cgaaatctca
    10321 tcggggtttt cccgcagttt tttgatatcc agcatatgca gctctttgcg aaggacctac
    10381 tgtgccgcgc ccggctccag cagggcgttt ttcgcctcca ggatttttct cacgcggtcc
    10441 tggcacggcg ggcagtaatt ccgccccttt gcgtccagcc gctccatgtc catataggca
    10501 gacagggcgc acagcggatt cgggcagatt tccatgccca gcaggatgcc gcccgcgctc
    10561 atcagctgtt tctcgacccg gcggccgtaa agctccgcat ccatgggatc cgggcgcagc
    10621 gcggccacat tgatcaccgc aacagactgc tcaggatacg aggcgccgtg atgcggattc
    10681 ccttccagtg acgccagcac gagcaccgca agatggttct ccttcttccg ggcaacggcg
    10741 gcctgcgcct gcccgtcaag agccccctcg gccagctcca tgggctcaag aagcataacc
    10801 gtcgtgaaaa ggttggcctg cacatacgcc tgcacccggg cggcgagatc ggcctccacc
    10861 tcaccggccg tcaccagcgc gacgccgtta accggcgcgg cacacggctc cggttccggg
    10921 gcggcttcct gcgcggggat ttcgattgtc ccggccgctt cgggggaaga ttcctccacg
    10981 gcggcttcaa ccgctgcggc cgccgcttcg acaaccgcag gcgccgattc tccgctcaca
    11041 gcctccacgg gttcctgagc ctggacaagg ccggacatgc aaagcgcgac aaagaaactc
    11101 aaaaacagtc tcataagaaa aactcccatt gatatccgct gaaaaactcc gccattcata
    11161 tatgaatccc atccgcaatg caacaagcga aaaacgcaaa aaatgaccaa aaaacgtaaa
    11221 aaataacgaa aaattggcct tttttggccg tttttagccg tttttgggca ttttttgcgc
    11281 tttttgagcc tttttgcggg agaacaagaa gatattcgcg
//
LOCUS       DAOQWR010000082        11283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6209_length_11283_cov_5.321518, whole genome shotgun sequence.
ACCESSION   DAOQWR010000082 DAOQWR010000000
VERSION     DAOQWR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6209_length_11283_cov_5.321518"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKK36_07565"
     CDS             <1..349
                     /locus_tag="PKK36_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94450.1"
                     /translation="DLLKDGFDVDVSHAFSRFFSEATDVLVRYAAGYVLTGDPDSEEF
                     RQKACRYFGCTMPLYWLAAALDCLLFMTRGYTLVTRGRRRIWRPRRSVVLLDGRTLAE
                     AALTAKIGTAAEK"
     gene            440..580
                     /gene="rpmH"
                     /locus_tag="PKK36_07570"
     CDS             440..580
                     /gene="rpmH"
                     /locus_tag="PKK36_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015363895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNR94451.1"
                     /translation="MTTKRTYQPSKKKRQRKHGFRSRMATRGGRDVLSRRRGKGRKSL
                     SV"
     gene            610..1008
                     /gene="rnpA"
                     /locus_tag="PKK36_07575"
     CDS             610..1008
                     /gene="rnpA"
                     /locus_tag="PKK36_07575"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNR94452.1"
                     /translation="MPIEGQSGSSCVPVRETLPRRCRLSSSALFREAYDGGRHWVGDF
                     MVMWLRTGPGASLRLGVVASRKVGNAVHRARAKRRLREAWRRSRSRFRGDYDVVLVAR
                     RSIISASWESVVTDLLRLAGRAGLTKGTKG"
     gene            1010..1252
                     /gene="yidD"
                     /locus_tag="PKK36_07580"
     CDS             1010..1252
                     /gene="yidD"
                     /locus_tag="PKK36_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNR94453.1"
                     /translation="MRRLAIALIRGYQRFISPWLGEHCRFYPTCSSYAVEALEQHGFF
                     SGLRLALLRLLKCHPFHPGGPDPVPPINGPTRRIAR"
     gene            1249..2994
                     /gene="yidC"
                     /locus_tag="PKK36_07585"
     CDS             1249..2994
                     /gene="yidC"
                     /locus_tag="PKK36_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNR94454.1"
                     /translation="MKKNDIILIAVLVGLLMLWPKVDQYIIKPLVFKRPAVEVFPEPE
                     PAPETESAALPVPAAVVEAAPSAPAVLAAPEPESEEAVPEEMLVLENEYIRLELSSHG
                     GGIASVTIKTYRQTLDTGSDPVHMEFSSMRPFEYSASRAAFRVENANDREAVLTLPGP
                     GGLVLTRTYELGERYDLSCTEVWRNPGGEAAVLPPQQMELGAMQDFETGPVMKGLYVL
                     GVDTLSPGGEKVRYWGKKIGSLFKDEVKEKRLEKLPEEILLEPRRSPVDWVAVKNKYF
                     VQILAPEGGAEGFTLYARRELDPREIENPSFAPRMQPLKEAGALIHLPEVVVAAGGEF
                     RRAISGYIGPKKYDELAGRGMDQVEVMEFGMWAPVGKLLLKIMNSIHRRLWPHNYGLA
                     IILLTIIIRVVFWPITHKSTESMKKMSEIQPQVALIREKYKDNPQKMQQATMALYKEN
                     KVNPVGGCLPMLIQIPVFIALFVVLRSAIELRFASFLWIRDLSQAENLLAGVLPIPLN
                     ILPLVMAATMWLQQKMTPQAGDPQQQKVMAFMPVMMLFLFYKMASGLVLYWTTNQCLM
                     ILQQFMMKRKQAKKA"
     gene            3009..3503
                     /locus_tag="PKK36_07590"
     CDS             3009..3503
                     /locus_tag="PKK36_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNR94455.1"
                     /translation="MSGTSQFTGAALKELETILRLMGVEAKVDAYDEAEEIYLHIESP
                     DAGRLIGKGACGLESLQYILNRVLLRKYPEVPHSRVDIERYRERRGDRLRHEALDAAE
                     RVLETGRPYRFNPLHSFERKLVHQLIKRRDGLATYSEESSGDGRKVLVICRAGDEAGA
                     SAAG"
     gene            3847..5301
                     /locus_tag="PKK36_07595"
     CDS             3847..5301
                     /locus_tag="PKK36_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94456.1"
                     /translation="MGWSVFYYNSEHPVAAWVLIVAEVGCLWLFVLAGTTLWRLRFKP
                     PEGVEVKETPSVDILIPTCNEPENVIRKTLTAVSKIRWDGPLKVYILDDGGTDVVENM
                     AKEFGYTYLSRRRSGEDLSDAKSGNLNFGYKNSRSDMIMTLDADQVPDPEILNKLAGY
                     AFFKNVAFVQSKQQYITQIGDPFYNSSKVFYDTVELGMDNMDTAISAGTGVLYWRKAL
                     DEIGGFVTWNLVEDLTTSMELHSHGWRSFYFPHALSRGLAPDSIWKVYQQRGQWALDT
                     MRLFWWQNPMFKKGLPLRSRLHYSTVASSYIFSALALPFFFLMPIWTYLTGDAVFEDD
                     FQTYLWIRGIYFFFMMAAIHFMCYKEAPAKQFRHLAGLFPLYFKNIFKALLYPKGRKP
                     KYRPNNSRTDLPKLEPRPHILAVLPQLLVIGLNAVVPFYATYYQTCPPRLIAGNAFLS
                     AFAIWALSDIVAAALRPKRWPEGGSPDDIYGKIQ"
     gene            5276..7975
                     /locus_tag="PKK36_07600"
     CDS             5276..7975
                     /locus_tag="PKK36_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94457.1"
                     /translation="MTSTEKSSKTGWPTKLFIAFWGVALLAVLCWYAPEFWRDAVDYY
                     NKAVSLQEKGRYEEALKPLDRAIRMDPENAGYLTTRGNIQLAMGDYAGAEDTFREVSV
                     LDPDEAEALFGMVEAARRQERRDEALKGLDQMAGMTLTREQALRRAQIYADLQAQAPA
                     LAAVTDLLKETPDDPALLKESAALKSALNDFSGAAGDYTRLAAVLKEGEEQRAALTGA
                     AVSHRAAKNPQEAWKIFQQIAGKDNLQARAELASELGQNSEAAALYEELLADDPGNAV
                     NGRALAVLYESMKEHIKAANRLEKVQAIEPNPEQVLKIAHLYRWGDKPEKAVPFYKLY
                     IKECAADRLKAVPAHEGAARALIDLKRGEQAMEHLNLLLDLAPKNPEVLVLAARAADL
                     TGNPALTVEYLEKLEAVRALSRDEAVWLAGQLRLADRKKESLARYEAMLASPDFAGNV
                     LIIEAVADLRADFNRYAEALKAYAMIPAGKIEPIHHLKIGRAAEKAGEVDLAIAEYGK
                     YLEVKKGDLEARLAMASLLDLQGKTEEAGKLMAEVYPQIKAELEKKPRDEMLLLLGAR
                     AASMSQDPAGTIGYLERLSAVRALTFAERIWQAGQTRAAGEAEKALNLYEALYAESGD
                     MFDGVALEALGDLRQDFDNFPGALAAYEQIREDQRPKTLSLKLARVSARQADQAEGAV
                     KYYTEYLKAFPGDTDAQLEAARIFVNLGHQQESLALYEKYTEAKGKTGLGLEVARAYL
                     SAQQFARAEEWSRFAVEEEGATNWVPHLCLAQSLHLQGKVKDADDIIEDYREEMEKTR
                     EGLEWLGLVAIARDRHLEALRTFDKLVENEDWVRLKYWIWRARAATRLGDLSRAKESI
                     EKAREFVAKGGGQTMESAEREADAALEQLKGAEKKGRQKDVKE"
     gene            7962..9011
                     /locus_tag="PKK36_07605"
     CDS             7962..9011
                     /locus_tag="PKK36_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94458.1"
                     /translation="MSRNSIWLLVALLAGMPAARAFEYDEDPKVIEVANAELKAAMSP
                     EVSALGNAWWDDNDISFLQWGVEARVWPSAGLPLRLQFLDGEIEQDSSAVDTNFNRTA
                     FALVLDGYRPVPSVGLKGRIMYEDFDDDDLLGGQAEIRVYRESGSEYALYGASESLWA
                     QNNVRNPRQYPRVSDLSQLDQNLRVNTAGGTLDFAPSLEQQVRLNVSWSEYEEDINGD
                     NNERLSAYVHWQVPILNSTSEKWLTIRPNIYYEHFRDELPGYYSPDYHASAGIGLHTV
                     RKYAHKQKLEIEVKPEWIFRNPDGEDEDTTAIHVVLDYSVEIKKLLLGAGGFIYAEED
                     GYWLGVVSARAGFRF"
     gene            9028..10146
                     /locus_tag="PKK36_07610"
     CDS             9028..10146
                     /locus_tag="PKK36_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNR94459.1"
                     /translation="MHAVLVLLCAAGGAAESVLTDDEFLREEWKTYRSSYIRRDGYVF
                     DPHNNGGEVTSEGQSYALLRAVWMDDEQTFARVLRWTEKNLKRSDGMYAWQWHPEDGT
                     KDSNTATDADQDIALALILAAHHFDRPEYLALARDIIVAVREHAGIKIGNGWFPSAGN
                     WAVEDRVINLSYFMPYSYPFFDRLDPEGNWMQVLETGYDLIEQARRLPGVMLIPDFME
                     LDENGVLKPVAEYRGLSGNYSFDSMRIHWRVALDCRLNKRPRACADPAGTRELARLLR
                     ENHKIVLRYTPDAVPMSHTESLSFYGSLLPAFEQIAPESAQRIRQKELSFRVLKRLMK
                     KKDRYYDRNWVWFGFAASSGLIERKMPPASSQKQSCGL"
     gene            complement(10605..>11283)
                     /locus_tag="PKK36_07615"
     CDS             complement(10605..>11283)
                     /locus_tag="PKK36_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNR94460.1"
                     /translation="TFESFVGMISAPLIYGMTPAETALFLKKQMMPDVSLYAVPMSGW
                     HRSAGWPGLAPWNPPSPGILTWETAMIYPSLVCCEALPMLDCGRGTQNIFRIVAAPWI
                     RAEELREKLGQLPGLDTSIEKYRSGRGRSAGAEMEGLRFAVISPENLRPVETAVRLLA
                     AIQDLYGTGRLWSDPETRPEFFDKLMGTASVRESLQNSIPVPQIAAAWKSAANRFKAV
                     RESVLLY"
BASE COUNT         2370 a         3160 c         3544 g         2209 t
ORIGIN      
        1 tgacctgctg aaggacgggt tcgatgtgga tgtatcgcac gccttttccc gattcttctc
       61 cgaggccacg gatgtgctgg tgcgctacgc ggccggatac gtgctgacag gcgacccgga
      121 cagcgaggaa ttccgtcaga aggcctgccg ctacttcggc tgcaccatgc cgttgtactg
      181 gctggccgcg gcgctggact gcctgctttt catgacccgc ggctacacgc ttgtcacgcg
      241 cggccgccgc cggatctggc ggccccgccg ttcggtcgtg ctgctggacg gccggacgct
      301 ggcggaagcc gcgctcaccg ccaagatcgg cacagcggct gaaaaatagg ttctgccggg
      361 cggaattcag ccgtaaaggg ttgacagtcc gttcgaaact gttactttcc atcggttttt
      421 aaataaaaag ggatcagcaa tgacgacgaa acggacatat cagccttcga agaagaaaag
      481 acagcgcaag catggcttcc gcagccgcat ggccacgcgc ggcggccgtg acgtgctgag
      541 ccgccgcaga ggcaagggac gcaagtctct ttccgtctga tcatggattc caaaacgggg
      601 agttttcctg tgcccattga gggccagtcc ggctcttcgt gcgtccctgt gcgggaaact
      661 cttccgcgcc gctgccgcct ttcctcgtcc gctcttttcc gcgaagcata tgacggcggc
      721 cgccactggg tgggcgactt catggtcatg tggctccgga caggacccgg cgcttcgctt
      781 cgtctggggg ttgtggccag ccgcaaggtg ggcaatgccg tgcatcgcgc gcgcgccaag
      841 cgcaggcttc gtgaggcctg gcggcgcagc cgcagtcgtt tccgcggcga ttatgatgtt
      901 gtgctggtcg cccggcggtc tattatttca gcttcatggg aaagcgtggt gaccgacctg
      961 ctccggctgg cgggccgcgc cggactgaca aaaggaacaa agggataaaa tgcgcagact
     1021 ggccatagcg ctgatcagag gctatcagcg gtttatttcg ccgtggctgg gtgaacactg
     1081 ccggttttat ccgacctgct ccagttatgc cgttgaggcg ctggaacagc atggattttt
     1141 ttcaggactg cggctggcgc tgctccggct gctgaaatgc catccgtttc atcccggggg
     1201 gcctgatccg gttcctccga taaacggccc gacaagaagg attgccagat gaagaaaaat
     1261 gacatcattc tgattgcggt gctggtgggc ctgctgatgc tttggccgaa ggtggaccag
     1321 tacatcatta agccgctggt tttcaagcgg ccggcggtgg aggtattccc cgaaccagaa
     1381 cctgcgcccg agaccgaatc cgccgccctg cctgtgccgg cggcggtcgt tgaggcggcg
     1441 ccctccgcgc cggcggtcct ggccgcgccc gagccggaat cggaggaagc agttccggaa
     1501 gagatgctcg tgctggaaaa tgaatacatt cgtcttgagc tttccagcca tggcggcggc
     1561 atcgcctcgg ttacgatcaa aacctaccgc cagacactgg acacagggag tgatccggtg
     1621 cacatggaat tttcctcgat gcggcctttt gaatattcgg cgtcgcgggc ggctttccgc
     1681 gtcgagaatg cgaatgatcg cgaagcggtc ctgacgctcc ccggcccggg cggactggtt
     1741 ctgacccgga cctatgaact gggcgagcgc tatgacctgt cctgcaccga agtctggcgc
     1801 aatccgggcg gcgaagcggc cgtgctgcct ccgcagcaga tggaactcgg cgccatgcag
     1861 gatttcgaaa cagggccagt gatgaagggt ctctatgtgc tgggagtgga cacgctttcg
     1921 ccgggcggag agaaggtccg ctactggggc aagaaaatcg gttcgctctt caaggatgag
     1981 gtgaaggaaa aacgcctcga aaaacttccg gaggaaattc tgctggagcc ccgccggtcg
     2041 ccggtggact gggtggcggt caaaaacaag tattttgtgc agattcttgc gcccgagggc
     2101 ggcgcggagg gcttcacttt gtatgcgcgc cgcgagctgg atccgcggga aatcgaaaat
     2161 ccgtcctttg cccccaggat gcagcccctg aaggaggcgg gcgccctgat ccaccttccg
     2221 gaagtggtgg tggccgccgg cggcgaattc aggcgggcaa tcagcggtta catcgggcct
     2281 aagaaatacg acgaactggc cggccgcggc atggaccagg tagaggtcat ggagtttggc
     2341 atgtgggcgc cggtgggcaa gcttctcctg aaaatcatga actccattca ccgccggctg
     2401 tggccgcaca actatggcct ggccattatc ctgctgacca ttatcatccg ggttgtgttc
     2461 tggccgatca cgcacaagag caccgagagc atgaaaaaga tgtctgaaat tcagccgcag
     2521 gtcgcgctga tccgggaaaa atacaaggac aacccccaga agatgcagca ggccaccatg
     2581 gcgctctata aggagaacaa ggtgaatccg gtcggcggct gcctgccgat gctgatccag
     2641 atcccggtat tcattgcgct gtttgtggtt ctgcgcagcg ccattgaact gcggttcgcc
     2701 tccttcctgt ggattcgcga cctgagtcag gcggagaatc ttctggcggg tgttcttccg
     2761 atcccgctga acatccttcc gctggtgatg gccgccacca tgtggctgca gcagaaaatg
     2821 acgccgcagg cgggcgatcc gcagcagcag aaggtgatgg ccttcatgcc ggtcatgatg
     2881 ctgtttctgt tctataaaat ggcgtcgggg ctggttttgt attggacaac aaaccagtgt
     2941 ctgatgatac ttcagcagtt catgatgaag cgcaaacagg cgaaaaaagc ctgatgggag
     3001 acagagacat gagcggaaca tcacagttca ccggggccgc cctcaaggaa ctggagacga
     3061 ttctccggct gatgggcgtg gaagcaaaag tggacgccta cgacgaggcc gaggaaatct
     3121 atctgcacat cgagtcgccc gatgcgggcc gcctgatcgg gaagggcgcc tgcggtctgg
     3181 aatcccttca gtatatcctg aaccgggtgc tgctgcggaa atatcccgag gttccgcaca
     3241 gccgcgtgga tattgagcgc taccgcgagc gccgcggtga ccggcttcgc cacgaggcgc
     3301 tggatgctgc tgaacgcgtg ctggaaaccg gccggccgta ccgcttcaat cctctgcatt
     3361 ccttcgagcg gaagctggtg catcagctga tcaaacgccg ggacggtctg gcgacctatt
     3421 ccgaggaatc ctccggcgac ggccgcaagg tgctggtgat ctgtcgcgcc ggcgatgagg
     3481 ccggcgcatc cgcagccggc tgattttttc ccgtgtcatc cgggtggtgt ctcaaggcgc
     3541 ggattagctg cgcaacctgc ttgatatgag caggttccgt ccggcttctg agagcagggg
     3601 tttccggcgc atgcgcaaac aagccgcgaa atatagactc aacgctttga cttttccttg
     3661 atttgctata gactgaaagg cagaggagat catgagttgc tcttctgcga ggagcagttg
     3721 aaggaattga ggtgaaaaat gaagtctcag ccgtctttta gaagatacca tgaagctgaa
     3781 gaccgtcccc ggagaatagc cgcccggctt ttgtcgcttt tgacatattt caccgggctt
     3841 ttttatttgg gatggtccgt attctattac aactctgaac atccggtcgc ggcatgggtc
     3901 ctgattgtgg ccgaggtcgg ctgcctctgg ctgttcgtcc tggcgggcac caccctgtgg
     3961 cgcctgcggt tcaagccgcc cgagggcgtt gaagtcaagg aaaccccgtc ggtggacatc
     4021 ctgattccga cctgcaacga gccggaaaat gtgattcgca agacgttgac cgctgtgtcg
     4081 aagatcaggt gggacggtcc gctcaaggta tatatactgg acgacggcgg aacggatgtg
     4141 gttgaaaaca tggcgaagga gtttggctac acctatcttt cgcgcaggag atcgggcgag
     4201 gatctgagcg atgccaagag tggaaacctg aatttcggct acaaaaacag cagaagcgac
     4261 atgatcatga cgctcgatgc cgaccaggtg cccgaccccg aaatcctgaa caaactggcg
     4321 ggctacgcgt tcttcaaaaa tgtggccttt gtccagtcca agcagcagta catcacccag
     4381 atcggcgatc cgttctacaa cagctccaag gtgttttatg acacggttga gctgggcatg
     4441 gacaacatgg atacggcgat ttcggccggc accggcgttc tgtactggcg caaggcgctg
     4501 gatgaaatag gcggatttgt gacgtggaac cttgtggagg acctgaccac ctcgatggaa
     4561 ctgcacagtc acggatggcg ttcgttttat ttcccgcacg cgctgagcag ggggctcgcg
     4621 cccgactcca tctggaaggt gtatcagcag cgcggccagt gggcgctgga cacgatgcgc
     4681 cttttctggt ggcagaaccc gatgttcaag aagggccttc cgctgcggag ccggctgcat
     4741 tacagcacgg tggcttcgtc ctacatcttc tccgcgctgg cgctgccctt cttcttcctg
     4801 atgccgatct ggacttatct gaccggcgac gcggtgttcg aggacgactt ccagacctat
     4861 ttgtggatcc gcggcatcta tttcttcttc atgatggccg cgattcactt catgtgctac
     4921 aaggaggcgc ccgcgaagca gttccggcac ctggccgggc tgttcccgct gtatttcaag
     4981 aacatcttca aggcgctgct gtatccgaag ggccgcaagc cgaaataccg gcccaacaac
     5041 tcgcgcaccg acctgccgaa gcttgagccc aggcctcata tcctggcggt gctgccgcag
     5101 ctgctggtga tcggtctgaa tgcggtggtg ccgttctatg ccacctatta ccagacctgc
     5161 ccgccgcgac tgatcgcggg caatgcattc ctgtccgctt ttgcaatatg ggcgctgtcc
     5221 gacattgtgg ccgccgcgct caggccgaaa cgttggccgg aaggagggag tcccgatgac
     5281 atctacggaa aaatccagta aaacaggttg gcccacgaag cttttcatag cgttctgggg
     5341 cgtggcgctg ctggcagtgc tatgctggta cgcgcccgag ttctggcgcg atgcagtgga
     5401 ctactacaac aaggctgttt cgctgcagga aaagggccgc tatgaagaag cgctcaaacc
     5461 gctggaccgg gccatccgga tggatccgga gaacgccgga tacctgacca cccgcggaaa
     5521 catccagctt gccatgggag attatgccgg agcggaggac accttccggg aggtgtccgt
     5581 gctggatccg gacgaggcgg aagccctgtt cggcatggtg gaggccgccc gccgccagga
     5641 acggcgggac gaggcgctga agggactgga ccagatggcc ggcatgaccc tcacgcgcga
     5701 acaggcgctg cgccgcgcgc agatctatgc cgacctgcag gcgcaggcgc ccgcgctggc
     5761 cgctgtgaca gacctgctga aagagacccc ggatgatccg gcgctcctga aagagtccgc
     5821 cgcgctgaag agcgcgctga acgatttttc cggcgcggcc ggcgattaca cccggcttgc
     5881 agcggtgctg aaagaaggag aggaacagcg cgccgcgctg accggcgcgg ccgtgtcgca
     5941 ccgcgcggcg aagaatccgc aggaggcatg gaaaatattt cagcagattg ccggaaagga
     6001 caacctgcag gcgcgcgccg agctggcatc ggagctggga cagaacagcg aagcggcggc
     6061 tctttatgaa gagctgctgg ccgatgatcc tggaaacgcg gtcaacggcc gggcgctggc
     6121 ggtcctgtat gaatccatga aggagcatat caaggcggcc aaccggctgg aaaaggttca
     6181 ggcgatcgag ccgaaccccg agcaggtgct gaagattgcc catttgtacc gctgggggga
     6241 caagcccgaa aaggctgtgc cgttctacaa gctttatatc aaggagtgcg cagcggaccg
     6301 cctcaaggcg gttccggccc acgagggtgc cgcccgcgcg ctgattgatc tgaagcgagg
     6361 cgagcaggcg atggagcacc tgaacctcct gctggatctg gcgccgaaaa atcccgaggt
     6421 attggttctg gcggcccgtg cggccgacct gacgggcaat ccggcgctga ctgtcgaata
     6481 tcttgaaaaa ctggaggcgg tccgcgccct gtcgcgcgat gaagccgtct ggctggccgg
     6541 gcagctccgg ctggcggacc ggaaaaagga gtcgctcgcg cgctatgaag cgatgctggc
     6601 ctcgcctgat tttgcgggga acgtcctgat catcgaggcg gtggccgacc tgcgcgcgga
     6661 cttcaaccgc tatgccgagg cgctcaaggc ctacgcgatg attccggccg gaaaaattga
     6721 gccgatccat catctgaaaa tcggccgtgc cgccgagaag gccggcgaag tggatctggc
     6781 catcgcggaa tacgggaaat atctggaggt caagaagggt gatcttgagg cgcgcctcgc
     6841 catggcttca ctgctggacc tgcagggcaa gaccgaagag gccggaaagc tgatggcgga
     6901 ggtttatccg cagatcaagg cggaactgga gaagaagccg cgggatgaaa tgctcctgct
     6961 gctgggggcg cgcgcggcca gtatgagtca ggatcccgct ggaacgattg gctatctgga
     7021 gcggctgtcg gccgtccgcg cgctgacctt cgccgagcgc atctggcagg ccggccagac
     7081 ccgcgccgcc ggcgaggccg agaaggcgct gaatctgtac gaggccctct atgcggagtc
     7141 aggcgacatg ttcgacggcg tcgcgctgga agccctgggc gacctgcggc aggacttcga
     7201 caatttcccc ggcgcgctgg ccgcgtatga gcagatccgc gaagaccagc gtcccaaaac
     7261 cctgtcgctg aagctggcgc gggtttccgc gcggcaggcc gatcaggctg agggcgcggt
     7321 gaaatactat accgaatatc tgaaggcgtt tcccggcgac acggatgccc agcttgaggc
     7381 cgcgcgcatt ttcgtgaacc ttggacacca gcaggagagc ctggcgctgt atgagaagta
     7441 taccgaggcg aagggaaaga ccggcctcgg ccttgaggtg gcgcgcgcct atctgtctgc
     7501 ccagcagttt gcgcgggccg aggaatggtc gcgtttcgcg gttgaagagg agggcgccac
     7561 caactgggtg ccgcatttgt gccttgctca gtcgcttcac ctgcagggca aagtgaagga
     7621 tgccgatgat attatcgaag actaccgcga ggagatggaa aagacccgcg aaggtttgga
     7681 gtggctgggc ctggtggcca tcgcccgcga ccggcatctg gaggccctga ggacattcga
     7741 caagctggtg gaaaatgagg attgggtccg cctgaaatac tggatctggc gcgcgcgcgc
     7801 cgcgacccgg ctgggggacc tgtcccgcgc gaaggaaagc atcgaaaaag caagagagtt
     7861 tgtcgcgaaa ggcggcggcc agaccatgga aagcgcggag cgcgaggcgg acgcagccct
     7921 ggagcagctt aaaggagccg aaaagaaagg aaggcagaaa gatgtcaagg aatagcatat
     7981 ggttgctggt ggcgctgctc gccggaatgc cggcggcgcg cgcctttgaa tatgacgagg
     8041 atccgaaggt gattgaagtc gccaatgcgg agcttaaggc ggccatgagc cccgaggtga
     8101 gcgccctggg caacgcctgg tgggatgaca atgacatctc cttcctgcag tggggcgtcg
     8161 aagctcgcgt ctggccttcc gcaggcctcc cgctgaggct gcagtttctg gatggcgaga
     8221 tcgagcagga ctcgagtgcg gtggacacca acttcaaccg cacggctttc gcccttgtgc
     8281 ttgacggcta tcgtccggtg ccttccgtcg gattgaaggg ccgcatcatg tatgaggatt
     8341 ttgacgacga cgacctgctg ggcggacagg ctgagattcg cgtttaccgc gagagcggct
     8401 cggaatatgc cctgtacggc gcatcggaat cgctctgggc ccagaacaat gtccgcaacc
     8461 cgcgccagta cccgcgcgtg agcgatcttt cgcagctcga ccagaatctg cgcgtgaaca
     8521 ccgcgggcgg cacactggac ttcgctccgt cccttgagca gcaggtccgc ctgaatgtga
     8581 gctggtcgga atatgaagag gacataaacg gcgacaacaa cgaaagactg tccgcctatg
     8641 ttcactggca ggtcccgatc ctgaacagca catctgaaaa atggctgacg atcaggccca
     8701 acatctatta cgagcatttc cgcgacgagc tgcccggcta ctacagcccg gattatcatg
     8761 cctcggccgg gatcggactg cacacggtcc gcaaatacgc acacaagcag aagctggaga
     8821 ttgaggtcaa gccggagtgg atattcagga accccgacgg cgaggatgaa gacaccacgg
     8881 cgattcatgt cgttctcgac tattccgtgg agatcaaaaa gctgctgctg ggcgccggcg
     8941 gtttcattta tgccgaggag gacggctact ggctgggtgt ggtgagcgcc agggccggct
     9001 tccgcttcta acgccatgca gcgcctgatg catgctgttc tggtcctgct ctgtgcggcg
     9061 ggcggtgcgg cagaatccgt tttgaccgat gacgaattcc tgcgcgaaga gtggaaaacc
     9121 taccggtcgt cctatatccg ccgcgacggc tatgtcttcg atccccacaa caacggaggg
     9181 gaggtaacct ccgaggggca gtcctatgcg ctgctccgcg cggtctggat ggacgatgaa
     9241 caaacctttg cccgcgtgtt gcgctggacg gaaaagaacc tgaaacggtc cgacggaatg
     9301 tatgcgtggc agtggcatcc cgaagacgga acaaaggatt ccaatacagc gaccgacgcc
     9361 gaccaggaca tcgcgctggc gctgattctc gccgcgcatc attttgacag gcccgagtat
     9421 cttgcccttg cccgtgatat cattgtggcc gtgcgagaac atgccggcat caaaatcggc
     9481 aacggctggt tcccgtcggc cggcaactgg gcggtcgagg accgcgtgat caatctttcc
     9541 tattttatgc cgtactccta tccctttttc gacaggcttg atcctgaggg taactggatg
     9601 caggtgctgg aaacggggta tgatctcatc gagcaggcgc gccggcttcc gggagtcatg
     9661 ctgattcccg atttcatgga gctggatgaa aacggcgttc tgaaacccgt ggccgaatac
     9721 cgcggactga gcgggaacta ttcctttgat tccatgcgca ttcactggcg cgtggcgctg
     9781 gactgccgcc tgaacaaacg gccgcgagcc tgcgctgatc cggcgggcac acgcgagctc
     9841 gcgagactgc tgcgggagaa ccacaaaatt gtcctgcgct acacgcccga tgccgtcccg
     9901 atgagccaca ccgaatcgct gagcttttac ggaagtcttc tgcccgcctt tgagcagatc
     9961 gcaccggaat ccgctcagag aatcaggcaa aaggaactgt ccttcagagt gctgaaacgc
    10021 ctgatgaaaa agaaagaccg ctactatgat cgcaactggg tctggtttgg ttttgcggcc
    10081 tcgtccggcc tgatcgaaag aaaaatgccg cccgcatcca gtcaaaagca gtcctgcggg
    10141 ctgtagtctt tcttccttca tgcaatccct tgaaatcgca aaaaacgcga aaaagcgttc
    10201 caaaatgatc aaatttgctt gattttcgtg aaaatcgccg cgttttttga ttttgggcgc
    10261 gaaaatcgtt catgcgcaat ttttgtcatt ttgcggcatg gatcccccaa aatcagcgca
    10321 tggctgtgaa aacgcgaaaa aatgcccaaa aacgctcgga aatggctaaa aatggccaaa
    10381 attcgtcaaa aataggcaaa aaatgacgaa aattgaccaa atttcgaaaa atttgaatat
    10441 taaggacaaa acgcggccgg ttcactgcga cgaacaatga ggagccatta tttattcttt
    10501 gtaaacacaa accgcagaag taacatgttc cataaatacc attcgcttaa cagatcgaga
    10561 gatgtagaac atggccatat atataagtga acagcgcaca ttattcagta gagcaagaca
    10621 ctttcccgga cagctttgaa gcggttggcg gcagacttcc acgcggcggc gatttggggt
    10681 acgggaatgc tgttctgaag agattcgcgg accgaggcgg ttcccatgag cttgtcgaaa
    10741 aattccgggc gcgtttctgg atcggaccag agcctgccgg tgccgtagag atcctgaatg
    10801 gccgcgagca gacggacagc cgtttcaacc gggcgcaggt tttcggggct gatcaccgcg
    10861 aagcgcaaac cctccatttc cgctcccgcg ctgcgccccc ggcccgacct gtatttttcg
    10921 attgatgtat caagtcctgg aagctgcccc agcttttccc tgagttcttc cgcgcggatc
    10981 cacggggccg ccacgatccg gaatatgttc tgtgttccgc ggccgcagtc gagcatcggg
    11041 agcgcctcgc agcagacgag cgagggatag atcatggccg tttcccatgt gaggattccc
    11101 ggcgacggcg gattccatgg tgcgaggccc ggccatccgg cgctccgatg ccatccggac
    11161 atgggaactg catacagcga aacatcgggc atcatctgct ttttcaggaa cagcgcggtc
    11221 tcggcggggg tcatgccgta gatcagcgga gcggagatca tgccgacaaa actttcaaaa
    11281 gtg
//
LOCUS       DAOQWR010000083        11247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6227_length_11247_cov_5.574696, whole genome shotgun sequence.
ACCESSION   DAOQWR010000083 DAOQWR010000000
VERSION     DAOQWR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6227_length_11247_cov_5.574696"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..834
                     /locus_tag="PKK36_07620"
     CDS             55..834
                     /locus_tag="PKK36_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNR94461.1"
                     /translation="MIAKDGTISLKWVGNLQIAGLNMREIEDAIRQAYEGSYLKDPQI
                     GVSMLGYGSVPVVVTGAVNKPGIYYLKSNTSTLLEILSLAGGLKADAGDELMLISEQM
                     QDPDEGMTNGIPVKKIVPVDIARLIDEGDFRVNMEVGANDIVTVRPNVQQVVYVLGYV
                     QRPGAFELKAGQRVDLVKGVALAGGLSPSARAENSYLVRETEQGQKVVPVNLARMAGG
                     DENMEYLQAGDTLVVGSGFMARLAEFVRPSVGAGFNYAPAP"
     gene            941..3205
                     /locus_tag="PKK36_07625"
     CDS             941..3205
                     /locus_tag="PKK36_07625"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNR94462.1"
                     /translation="MRNRVPEHGENYLRGEILEQKPYLRWLGVMRRHLWIILAAFLII
                     STITVLRAYRAVPVFQAVAKIMIERQAPTVTHFEGVVQPNVTWGAREYYRTQEQLARS
                     RAVLEIALERPEISAMFGEEAGDVPLRRTFRQTLSALLQMPAPEPPENWERLRGFISS
                     EQVRDTYFLYIRAEASNRQKAALLATAVAEAFVEFHRRNRRTASNDAFVYLEEQKEKE
                     ETALQLAQQKLQEFRQGTQSHSLDLSEVGHPVMKRLTLLNEQLTQAQMERINLEAELR
                     VVRRALASGTDAFQDVGEQVFSLSSIRKDPRMAEIRSALIAAEEEAATLADIYGPQHP
                     NYVAVSTRMNLLKGKLSEALLEIVESLTSRLEVVQGQEKELQAEYDQQNAAAMTLANQ
                     SLEFRNLQNEVDRHQKLYSVLLERMSEVELTSDYAKTNVELIEAAALPKAPVRPNKIS
                     MVVKGLLLGLVLGIALAFFVEYMDETVRSPEDIELRVGIPVLGFIPRIRLDREASDKE
                     ASRLLVYARGEDNIIMEAYRNLRTLVFFARPVEESKVLLMSSAGPGDGKTTTAANLAM
                     TIAKANRKVLLVDADFRRPMITKYFGLSAETGLSSVLAGESPLEDALQKTVVDLEVLN
                     NLDILSAGPMPPDSGELLDSSRMDRLLMELRDKYDRIIIDAPPVLYVTDASILAAKSD
                     GVILVIKAGRRTAAFVRRAFKHIENVQGKVIGGVLNMVNVPRFGHYYSDYYYYGYARK
                     DSAYYGSEKSRSGR"
     gene            3174..5219
                     /locus_tag="PKK36_07630"
     CDS             3174..5219
                     /locus_tag="PKK36_07630"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR94463.1"
                     /translation="MAPKKAGPAAETGNIGPAFNTARAHVGTLAGRISGWMTLAVVMA
                     APWLFSAGEAWVWLSLCIIVNLALILSLTNFYCRPESLRYAPVWTAAYPAILLFMFLQ
                     AVRFPEPLIARLSPLAADAVQVCRQIFADTELSGFLRQKGFPGLTGASISLAPLATRR
                     AFYLATAYFGVLVTIASHYSGWRRAQQAATLLAGTFFLLALFSITQHYSGTSRLYGYY
                     DPGYTGRIFGTFTNRNHHAAFMNMAFGLGLALVFSGTTWGDFRKLDSGRQRVSWLLKN
                     EGGLLIIKTFMTVVIGASACMALSRGAMVSLGASVVIIYLFSRLHLRGNHVSGHFVAM
                     FVIIFVSVLMWLGWEGVAQRMGSLLHVISDPMGNARVMALRDTLSLFAAMPVFGCGAG
                     AFRHVFPLFTNPDIGFGRWMHAHNDIAELLAEGGVVFMLLIGWAFFALFVRIWRGMED
                     SDDNGRLFVTGLLVGILAMLLHSLLDFSLHRPANPLLFAALVGFAIGGSQPDHDTPKI
                     RIARISSLRRRIHRGMAVLGILLIALISLTQVRELKGELAAARHHQWGRCALKSKGVK
                     QRQLCIREAVYEGQLIAAHGAGSPDQALKIAIRNIKWLRDTRLDPEVRIELADQALLL
                     TALAVQAAPTDFEYWLWLARACFIGNRPQAASVCLKRARQLAPVQQPVYLYLPPSGP"
     gene            5393..6559
                     /locus_tag="PKK36_07635"
     CDS             5393..6559
                     /locus_tag="PKK36_07635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNR94464.1"
                     /translation="MTTISLMALMALFTAVFATRLAIAAAPRLGMVDHPDGIRKTHSI
                     STPMLGGPALYVGIVVPMLLLWHLSGAVTTVANLFRHFTGQLLAILAGGTLALVLGLL
                     DDRFDLRPRWKLLGQCLIAVIIYASGMGIHAISNPFGGSLELGLLGFPVTVLWFMGCM
                     NAVNLMDGLDGLAAGICLFVGITLFLVSLHFHNVMGMVLLAVFNGAILGFLLFNFPPA
                     RIFLGDSGSMLLGYLVAAFSLLGASRKAEAAVALFIPIVALGLPIMDTAVAIVRRWYK
                     HLPIATPDRKHIHHRLVAMGYSPQRAVLVLYGASVVLAGFAVLITFGRSEVVVLVVAA
                     LVVITFVTVRIFSGVRLLDVQQKLKTDNEIRRRMAGINNIVDAVLSEIERAATL"
     gene            6596..6892
                     /locus_tag="PKK36_07640"
     CDS             6596..6892
                     /locus_tag="PKK36_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94465.1"
                     /translation="MELDRARISFRFPDQQRDETRQWMRSDVAAADRTGAEQDGWAFH
                     LNLRTETGRLLGRLEVEKYDLIIPEVTRFIERLRNAVEMRIPHCLEYHAAEDAS"
     gene            6889..7728
                     /locus_tag="PKK36_07645"
     CDS             6889..7728
                     /locus_tag="PKK36_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR94466.1"
                     /translation="MNGPATAEERIIRPRHGMVAINFRELWQFRELFAFLTWRDVLIR
                     YKQTAIGMMWAVLQPVLTMGILVFVFSRLAKMPDGGVPYPVLTFAAVLPWQLFSTAVA
                     SSSASVVGSAGMISKVYFPRLIIPGSASLSGAMDFAVSLAVLFLLMLIFQVPFRLHLL
                     LIPVFVLSAVLTAFAFGLWLSALNVKYRDVKYMVPFLLRMGMYISPVGFMSSLVPAKW
                     RFLYSLNPMVGVIDGFRWAILGPEFEPYWPGFGVSMAAVCLVLVSGLYFFRSTEKTFA
                     DII"
     gene            7730..8959
                     /locus_tag="PKK36_07650"
     CDS             7730..8959
                     /locus_tag="PKK36_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HNR94467.1"
                     /translation="MNTVITARGIGKRYQLGEHLRHNTLRDALRAAIHRPPPAGSGDF
                     WALKEVSFEIQRGEVVGVIGRNGAGKSTLLKILSQITDPTEGEIRIRGRVASLLEVGT
                     GFHPELSGRENVYLNGAILGMTRREISASFSDIAAFAGVEKFLDTPVKRYSSGMYVRL
                     AFAVAAHLNPEILLVDEVLAVGDASFQKKCIGKMDDVARSGRTILFVSHNMAAVESLC
                     AKTLLLDRGRVVAFGDTREVIDQYLSGASAAVSETDLRNHPGRPAGKMPLMSAFRLLK
                     GGGGPAPQTGLGEDIVFEVSGMTQETVLDAALNIQIMKPGGQRVATCYSRYQYPARID
                     LNGKFVFSCRMGNCRLMPGMYMLALILNGPGGPIDMIEGIPWEVVSRDIYGTGRVLPS
                     GTGFYLPEVEWSAKSNA"
     gene            8952..9998
                     /locus_tag="PKK36_07655"
     CDS             8952..9998
                     /locus_tag="PKK36_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR94468.1"
                     /translation="MPRKTILERIRSRLSWAFANHCRPRLLRAASRSAGLSRLYYLLF
                     SSAFRQEQHAVVSGMAEYFRNISSSASSNYLLRRNIHRLEKGMIMRPRRDIYALSYIR
                     ETVDAFCRACSGGETEELLWAASVLGAYFDCAKPHPLLDDLKTRFHSVQPKMPDACTP
                     SKMPSASAQVSYADLLSLMRSRKSVRWFKQLPVPRDILDQAVAAAALAPSSCNRQPFE
                     FRLIDDPVLVRKVAASPMGTEGFSENIPVIAVVIGHLQAFFSERDRHLIYIDSSLAAM
                     SFILALEALGVSTCCINWPDMAERKENLGAILRLKPCERPVMLIAAGYRDESGLVAWS
                     QRKPVEELRAYNLP"
     gene            9995..11095
                     /locus_tag="PKK36_07660"
     CDS             9995..11095
                     /locus_tag="PKK36_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNR94469.1"
                     /translation="MNIEIKGIGFGNRGAELMLIAILNRLRGSFPQARFGAATYAGTF
                     ARRTQYGLDHVVPDRWSIATLRHRMRRKEGRLICESDMDVLLDSSGYAYGDAWKTEWI
                     QNSAAHISRYRRRNRKIILLPQAFGPFANPSVRTAAETIIRSASLVYARDPESLEHVL
                     ALAPGASHVRLAPDFTGPLPGRLPEDAAADERRVCIVPNAEMLRICPDAYVPFMTRCI
                     RSVADRGYAPCILLHEGERDRELAARLCETSELPILDDADPQRLKGIIGSCAFIIGSR
                     YHGLVSAFSQGVPAMGTSWSHKYRHLFDDYGCPEMLIDLQSSDCFPDQLLNSLCDLES
                     RMALRGRLLEKSRAVNTKTEAMWREIETVLHS"
     gene            11096..>11247
                     /locus_tag="PKK36_07665"
     CDS             11096..>11247
                     /locus_tag="PKK36_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94470.1"
                     /translation="MNPLVSIIIPCFNAEKYLREAVESALAQTYRPIEVIVVDDGSTD
                     AGPEIAR"
BASE COUNT         2186 a         3048 c         3354 g         2659 t
ORIGIN      
        1 aaacgccctc tctcttgttg taggaattct gtctcacttt cattggcaca gagggtgatc
       61 gccaaagacg gaacaatctc cctgaaatgg gttggcaatc tgcagattgc cggtttgaac
      121 atgcgggaaa ttgaagatgc cattcgccag gcctacgaag ggtcatatct caaagatccc
      181 cagattggag tcagcatgct gggttacggg agtgtacccg ttgtggttac cggggccgtg
      241 aacaaaccgg gcatctatta tctgaaatcc aacaccagca cgcttcttga aattctttct
      301 ctggcgggcg gcctcaaagc cgatgcgggc gacgaactca tgctcatcag cgagcagatg
      361 caggatccgg acgaggggat gaccaacggc atcccggtca aaaaaatcgt tccggttgac
      421 atagcgcgcc tgattgatga gggcgacttc cgggtcaaca tggaagttgg cgccaacgac
      481 attgttactg ttcgtccaaa tgtccagcag gtggtgtatg tgctggggta cgtgcagcgt
      541 ccgggggcgt ttgaactcaa ggccgggcag cgggtggatc tggtgaaggg tgttgctctg
      601 gcgggcggtt taagcccgtc agcccgtgcg gaaaattcat atttggtgcg cgagacggag
      661 cagggtcaga aggttgtgcc ggttaatctg gcccggatgg ccggaggtga cgagaacatg
      721 gaatatctgc aggcgggaga tactctggtg gtgggctcgg gtttcatggc ccggctggcg
      781 gaatttgttc gacccagtgt gggggccggc ttcaattatg ctcctgcccc ctgatgagaa
      841 agcctgttaa taactttgac agagagacat ccagagtata tgttcaggac catacagggg
      901 agtgcggtgg tcttcaagta attgaggata aattggttat atgaggaata gagttccaga
      961 acatggagag aattatctca ggggggagat tctcgaacag aagccgtatt tgcgctggct
     1021 tggggtgatg agaaggcacc tgtggattat tctggctgcg tttctgatca tttcaaccat
     1081 cacagtcttg cgggcataca gggccgtccc ggtgtttcag gctgtggcca agatcatgat
     1141 tgagcggcag gctcccacgg tgactcattt tgaaggggtt gttcagccga atgtaacatg
     1201 gggtgcgcgg gagtactaca gaacgcagga gcagctggcc cgcagccggg cggttctgga
     1261 aatcgccctg gagcgccccg aaatcagcgc gatgttcggg gaagaagcag gcgatgtgcc
     1321 cctgcggcgg actttccggc agaccctttc agccctgctg cagatgcccg cgccggaacc
     1381 gccggaaaac tgggagcggc tgcgaggctt catttcttct gaacaggtgc gggacaccta
     1441 tttcctgtat attcgcgccg aggcatcgaa ccggcagaaa gcggctcttc tcgcaactgc
     1501 ggttgcagaa gcttttgtgg aatttcatcg ccggaaccgg cgcaccgcca gcaatgatgc
     1561 ctttgtatat ctggaagagc agaaagagaa agaggagacc gccctccagc ttgctcagca
     1621 gaagcttcag gaattcaggc agggcacaca gtctcactcg cttgatctgt cagaggtcgg
     1681 acatccggtt atgaagcgtc tcaccctgct taatgagcag ctgacccagg cgcagatgga
     1741 gcggatcaac cttgaggctg aactgcgggt ggtccggcgc gcgctggctt ccggtaccga
     1801 tgcctttcag gacgtgggcg agcaggtgtt ctctttgtcc tccatccgga aagacccccg
     1861 aatggcagag attcgcagtg cgcttatagc cgctgaagag gaagccgcca cccttgccga
     1921 catttatggc ccccagcatc cgaattatgt ggccgtcagc acccgcatga acctgctgaa
     1981 ggggaaactg tccgaggccc tgctggagat cgtcgaatcg ctgacatcgc gcctggaagt
     2041 ggtgcagggt caggaaaagg agctgcaggc tgagtatgat cagcagaatg cggcggcaat
     2101 gactctcgca aaccagtccc tggaattccg aaatctgcag aacgaagtgg atcgccacca
     2161 gaaactgtat tctgttcttc tggagcgcat gtcggaagtg gaactgactt ccgattacgc
     2221 caaaacaaat gtcgaactga ttgaagcggc cgctctgccg aaggccccgg ttcggcccaa
     2281 taagatttcc atggtggtga aggggctgct tctgggcctt gtgctcggca tagcattggc
     2341 gttctttgtc gaatatatgg atgagacggt ccggtctccg gaggacatag agctgcgcgt
     2401 gggcattccc gtgctgggtt ttattccccg tatccgcctg gaccgtgaag cctctgacaa
     2461 ggaagcctcc cgtttgctgg tgtatgcccg cggagaggac aatatcatca tggaggctta
     2521 tcgcaatttg aggacgctgg ttttcttcgc gcgtccggtt gaggagtcaa aagtgctgct
     2581 gatgagttct gccggaccgg gtgacggcaa aacaacaacg gccgcaaatc tggccatgac
     2641 cattgccaag gccaaccgga aagtcctgct ggtggatgcg gacttccgcc gcccgatgat
     2701 caccaaatac tttggattga gcgcggaaac gggcctcagc agtgtcctgg ccggagagtc
     2761 cccgttggag gatgcactgc agaagaccgt tgtggatctg gaagtgctga acaaccttga
     2821 tattctttcc gccgggccaa tgcctcccga ttccggagaa ctcctcgatt catcccgtat
     2881 ggaccggctc ctgatggagt tgagggataa atatgaccgg atcatcattg atgcgccgcc
     2941 ggtgctgtat gtcaccgacg ccagcattct ggcggcgaaa agcgacggcg tcattcttgt
     3001 tatcaaggcc ggccggcgta cggcggcgtt tgtccgccgg gccttcaagc acattgaaaa
     3061 cgtccaggga aaagttatcg gcggagtgct gaatatggtg aacgtgccgc gcttcggcca
     3121 ttattattcc gactattatt actacggcta cgcgcggaaa gattctgcat attatggctc
     3181 cgaaaaaagc aggtccggcc gctgagaccg gaaatatcgg gccggcattc aacacggctc
     3241 gggcgcatgt aggaaccctg gccggtcgaa tctccgggtg gatgacgctt gccgtggtga
     3301 tggcggctcc ctggctgttt tcggcggggg aggcctgggt ctggctctct ctgtgcatca
     3361 ttgtcaattt ggccctcatt ctttccctga ccaatttcta ctgtcgcccg gagagtctcc
     3421 gttatgctcc ggtgtggacg gccgcatatc ctgccatcct gctgtttatg tttctgcagg
     3481 ccgtccggtt tccggaaccc ctgattgcac ggctcagtcc gctggcggca gatgcggtgc
     3541 aggtttgccg gcagattttt gcggatacgg agttgagcgg tttcttaagg caaaagggat
     3601 ttcccggcct gacaggagca agcatatccc ttgcccccct tgccacgcgc agggcgttct
     3661 atctggccac ggcctatttc ggtgtcctgg taacaattgc atcgcattat tcgggctggc
     3721 ggcgcgcaca gcaggctgcg acgctgctcg ctggaacttt cttcctgctc gctcttttct
     3781 ccatcaccca gcattattcc gggaccagca ggctgtacgg gtactatgac cccgggtata
     3841 ccggccgcat cttcggaact tttaccaacc ggaatcatca cgcggcattc atgaacatgg
     3901 cgttcggcct tggtctggcg ctggtttttt cgggcacgac ctggggtgat ttccgcaagc
     3961 tggattccgg ccgccagcgg gtcagctggc ttttgaaaaa tgagggcgga ctgctgatca
     4021 ttaagacgtt tatgaccgtt gtgattggcg cttccgcatg catggcgctg tcgcgtggag
     4081 ccatggtcag tctgggagcc tccgtagtga tcatctatct gttttcccgg ctgcatcttc
     4141 ggggaaatca tgtctccggt cattttgtcg ccatgtttgt catcatcttc gtgtctgtcc
     4201 tcatgtggct gggatgggag ggcgtggcgc agcggatggg ctcccttctg catgtcatca
     4261 gtgatcccat gggcaatgcc cgggtcatgg cgctgagaga cactctcagc ctgtttgccg
     4321 cgatgccggt tttcggatgt ggagcgggcg ccttcaggca tgtatttccg ctttttacaa
     4381 atcccgatat cggtttcgga agatggatgc atgcgcataa tgacattgcg gaactgctgg
     4441 ccgagggagg tgtcgttttc atgctgctga tcggctgggc cttttttgcc ctttttgtca
     4501 gaatatggag gggcatggaa gacagcgatg acaacggccg tctttttgta acaggtttgc
     4561 tggtgggcat actggccatg ctgctgcaca gcctgctgga tttcagcctt catcggccgg
     4621 ccaacccgct gctgttcgcg gcgctggtag gatttgccat tggaggatcg cagccggatc
     4681 atgacacacc aaaaatccgg atagcgcgca tttcatcact gcggcgcagg attcaccggg
     4741 gcatggcagt ccttggcatt ctgctgattg cgctaatcag cctgacacag gtcagagagc
     4801 ttaaaggaga gctggcggcg gctcgccacc accagtgggg gagatgtgcg ctcaaatcca
     4861 agggggtaaa gcagcgccag ctgtgcatac gcgaagcggt ttatgaggga cagttgattg
     4921 ccgcgcatgg cgcgggcagt cccgaccagg cgctgaagat cgccattcgg aatataaaat
     4981 ggctgcgcga cacccgtttg gacccggaag tccgcataga actggctgat caggcgctgc
     5041 ttctgacggc gctggcggtt caagcggctc ccacggattt tgaatactgg ctgtggctgg
     5101 cccgtgcctg ttttatcggc aaccggccgc aggcggcatc ggtgtgcctg aaacgggccc
     5161 gccagctcgc gccggttcag cagccggtgt acctgtatct gcccccttca ggaccatgaa
     5221 gcgtcagcat cggcataatg atgacatata ttcaatattc tgctaaaatt gccagccaac
     5281 catgcaggat tttgtctgca tggaaatctg aggacatgtt gagccgttca cttgatgtca
     5341 atgcgaggga attgaccggc acgaagtgtg ccttcatatc cgggagcctg tcgtgaccac
     5401 gatcagcctg atggccttga tggccctgtt taccgcagtg tttgcgaccc gtctggccat
     5461 tgcggctgct ccccgactgg gtatggtgga tcatcccgac ggcattcgga aaacccattc
     5521 tatcagcacg cccatgctgg gcggacccgc actttacgtc ggcattgtcg tacccatgtt
     5581 gctgctctgg catttgtcgg gcgcggtgac caccgttgct aacctgttcc ggcatttcac
     5641 cggtcagctt ttggccattc tcgccggagg aacccttgca cttgttttgg gactgctgga
     5701 tgaccggttt gatctccgtc cccgctggaa acttctggga cagtgcctta ttgcagtgat
     5761 tatttatgcc agcgggatgg gcattcacgc catcagcaat cccttcggag gcagtctgga
     5821 gcttggtctg ctggggtttc ccgtgaccgt gctctggttc atgggctgca tgaacgccgt
     5881 gaatctcatg gacggactgg acgggctcgc ggcgggcatc tgtctttttg tgggcatcac
     5941 gctgtttctg gtcagcctcc attttcacaa cgtcatgggg atggtgctgc tggccgtgtt
     6001 caacggagcc attctgggtt tcctgctgtt caatttcccc cccgcgcgaa ttttccttgg
     6061 ggattcaggc agcatgctcc tcggctatct ggtggcggcg ttttctctgc tgggcgcttc
     6121 gcgaaaagcc gaggccgccg tcgcgctttt cattccgatt gtcgcgctgg gacttcccat
     6181 catggataca gcggtggcca ttgtccgccg ctggtacaag catcttccca ttgccactcc
     6241 ggaccggaaa catattcatc accgtctggt ggccatgggc tacagcccgc agagggctgt
     6301 gctcgtgctg tatggcgcct ccgtggtgct ggccggtttc gccgtgctga tcacttttgg
     6361 acgaagtgag gtggtcgtgc tcgttgtggc ggcgctggtg gtcataacgt tcgtcaccgt
     6421 ccgcattttc agcggggtcc gccttctgga tgttcagcag aaactgaaaa ccgacaacga
     6481 aattcgccgc cgcatggctg gcataaacaa tattgtggat gctgtgcttt cggagataga
     6541 gagggccgcc acactgtagc agctctgggc cgcctgcaca aaagcttttg agcaaatgga
     6601 actggatcgc gcccggatat cgtttcgttt ccccgatcag cagcgcgatg aaacccgtca
     6661 gtggatgcgc agcgacgtcg ctgcagcaga tcgtacaggc gctgaacagg acggctgggc
     6721 gttccatctc aacctgagaa cggagacagg ccgtcttctc ggccgcctcg aagtggaaaa
     6781 atatgatttg atcatacccg aagtaacacg atttatcgag cggctgcgca acgctgtcga
     6841 gatgcggatc cctcactgtc tcgaatatca tgccgcggaa gatgcatcat gaacgggccg
     6901 gcgacagcag aagagcgaat cattcgtccg cgtcacggaa tggtggccat caatttcagg
     6961 gaactctggc agttccggga gcttttcgca ttcctgacct ggcgtgatgt actgatccgc
     7021 tacaagcaaa ctgcgattgg catgatgtgg gccgttctcc agccggtatt aaccatgggc
     7081 attctggtat tcgtgttcag ccgtcttgcg aaaatgccgg acggaggggt gccgtatccg
     7141 gtgctgacgt ttgcggccgt tctcccctgg cagttgtttt caactgctgt ggcttcatcc
     7201 agcgcatccg ttgtgggatc ggctggaatg atcagcaagg tttacttccc gaggctgatc
     7261 atcccgggaa gcgcgtccct cagcggagcc atggatttcg cggtatccct ggctgtgctt
     7321 tttctgctga tgctgatctt tcaggtgccg ttccggctgc accttcttct gattcctgtt
     7381 tttgtgcttt cggccgtcct gacagccttt gcattcggac tttggctgag tgccctgaac
     7441 gtgaagtatc gcgacgtgaa atatatggtt ccctttctgc ttcgcatggg catgtatatc
     7501 agtccggtgg gttttatgag cagcctggtg cccgccaaat ggcggttcct ttacagtttg
     7561 aatccaatgg tcggtgtgat tgacggcttc cgatgggcca ttctcggccc cgagttcgaa
     7621 ccctattggc cgggtttcgg tgtgagtatg gccgccgtct gcctggtgct ggtttccgga
     7681 ctctacttct tcaggagcac ggaaaaaacc tttgcggata ttatctgaca tgaacaccgt
     7741 catcacagcc cgcggaatcg gcaagcgcta tcagcttggt gagcacttgc gtcacaacac
     7801 gcttcgtgat gccttgcggg cggctatcca ccggccgccc ccggccggat cgggagattt
     7861 ctgggccttg aaggaggtct cttttgaaat acagcgaggc gaggtggtcg gcgtgatcgg
     7921 gcgcaacggc gccggcaaat ccaccctcct caagatcctc agtcagatca ccgatcccac
     7981 ggaaggggaa atccgcatcc gcggacgtgt tgccagcctt ctggaagtgg gcacggggtt
     8041 tcacccggaa ctcagcgggc gcgagaatgt ctacctgaat ggcgccatcc tgggaatgac
     8101 ccgccgggaa atcagcgcca gtttctctga cattgccgca tttgccggag tggagaaatt
     8161 tctggataca ccggtcaaga gatattccag cggcatgtat gtgcgcctgg cgtttgccgt
     8221 cgccgcgcac ctgaatccgg aaatacttct tgtggatgag gtcctcgccg taggtgatgc
     8281 ctccttccag aaaaaatgca ttgggaaaat ggacgatgtt gcgcgcagcg gccgcacaat
     8341 cctctttgtg agccataaca tggcggccgt ggaatcgctt tgcgcgaaaa cccttctgtt
     8401 ggaccggggc cgcgttgtgg cgtttggcga cacccgcgag gtgatcgatc agtatctttc
     8461 cggggcctcc gcggcggtgt ccgagacaga ccttcgaaac catccgggcc ggccagccgg
     8521 gaaaatgccc ctcatgtcgg ccttccgtct tctgaaggga gggggcggtc cggcgcctca
     8581 aacgggcctg ggcgaagaca ttgtctttga agtgagcggc atgacacagg agactgttct
     8641 ggacgcggcg ctgaacattc agattatgaa acccggggga cagcgggtgg ccacctgcta
     8701 cagccgctat cagtatcccg cccgcatcga cctgaatggg aagtttgtat tttcctgccg
     8761 catgggcaac tgccggctga tgcccggaat gtatatgctg gcgctgatac tgaacggccc
     8821 tggcgggcca atagatatga ttgaagggat tccatgggag gtggtttcac gcgacatcta
     8881 tggtacgggc cgcgttctcc cttcgggcac cggcttctat cttcctgaag tcgaatggag
     8941 cgcgaaatcc aatgcctagg aaaaccatac tcgaacggat ccgcagtcgc ctgagctggg
     9001 cctttgcaaa tcactgcaga ccccgtctgc tgcgggcggc ctcgcggagc gcgggcttga
     9061 gccggcttta ctatcttttg ttttcatcgg ccttccggca ggagcagcat gccgtggttt
     9121 ccggaatggc ggagtatttc cgaaatatat cctcatctgc ttcctcaaac tatcttcttc
     9181 gaagaaacat tcatcgtctt gaaaagggca tgatcatgcg gccccgccgg gacatttatg
     9241 ctctttccta cattcgcgaa acagtggatg ccttctgccg tgcctgcagc ggaggcgaaa
     9301 ctgaggaact gttgtgggct gcgtccgtgc tgggcgcgta ttttgactgc gccaagcctc
     9361 atcctcttct cgatgatctc aagacacgct tccattctgt tcaaccgaaa atgccggacg
     9421 catgcactcc gtcgaaaatg ccgtctgcat ccgcgcaagt gtcttatgcg gatcttcttt
     9481 ccctgatgcg ctccagaaag tccgtgcgct ggttcaaaca gctccccgtg cctcgcgata
     9541 ttcttgatca ggctgtggcg gcggcggccc tggccccgag ttcctgtaat cgccagccgt
     9601 ttgaattccg cctgatcgac gaccctgtcc tggtgaggaa ggtggcggcc tctcccatgg
     9661 gcacagaggg gttttcggag aacataccgg tgatcgccgt tgtcataggt catctgcagg
     9721 cgtttttcag cgagcgggac aggcatttga tctacataga ttcctcgctg gccgccatgt
     9781 cgtttattct cgccctggag gcgctgggcg tctccacatg ctgcatcaac tggcccgata
     9841 tggcggagcg caaggagaat cttggcgcga ttctccggct gaaaccctgc gagcggcccg
     9901 tcatgctgat agcggcggga tatcgcgacg aaagcggcct ggtggcctgg tcgcagcgca
     9961 agccggttga agagctgcgc gcctacaacc tcccatgaac attgaaatca aaggcatcgg
    10021 tttcggaaac cgcggcgcgg aactcatgct gattgctatt ctgaaccggt tgaggggatc
    10081 tttcccgcag gcccgctttg gcgctgcaac ctatgccggg accttcgcgc ggcgtacaca
    10141 gtatggcctg gatcatgttg tgccggatcg atggagcatc gcgacacttc gccatcgcat
    10201 gcgcagaaaa gaaggccggc ttatttgtga atcagatatg gatgttctgc tggattcgtc
    10261 gggctatgcc tacggggatg catggaagac tgaatggatt cagaacagcg cagcccatat
    10321 ttcgcgatat cgccggcgga acaggaagat catacttctg ccgcaggcct ttggtccctt
    10381 tgcgaatcct tctgtgcgca cggctgctga aacaatcatt cgttcggcca gtctggttta
    10441 tgcgcgcgac ccggaatcac ttgagcatgt cctcgcactg gctcccggcg ccagccatgt
    10501 ccgtcttgct ccggatttta caggcccgct gcccggccgc ctgccggagg atgcggcagc
    10561 agatgagcgc cgtgtctgta ttgtgcctaa tgcggaaatg ctgcgcatct gtcccgatgc
    10621 gtatgtcccc ttcatgacga gatgcattcg gtcggtggca gacagggggt atgccccctg
    10681 tattctcctt catgagggcg agcgtgaccg ggaactcgcg gccagacttt gtgagacgtc
    10741 tgaacttcca attctggatg atgctgatcc gcagcgcctc aaggggatta tcgggagctg
    10801 cgcctttatc atcggctcgc gctatcacgg cctggtcagc gctttttcac agggagtccc
    10861 ggccatggga acgtcctgga gtcataaata ccggcatctc tttgatgact acggatgtcc
    10921 tgaaatgctg atcgatttgc agtcttctga ctgtttcccg gatcagctgc tgaacagtct
    10981 ctgcgatctt gaaagccgaa tggcccttcg gggccggctg ctggagaaaa gccgcgccgt
    11041 caataccaaa actgaggcaa tgtggcggga aatcgaaacc gtgctccatt cctgaatgaa
    11101 tcctctggtt tccattatta ttccctgctt caatgcggag aagtatctcc gagaagcggt
    11161 ggaaagcgct ttggcccaga cctaccgccc gattgaagtc attgtggtgg atgacggttc
    11221 aaccgatgcc ggaccggaga ttgcccg
//
LOCUS       DAOQWR010000084        10931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6436_length_10931_cov_6.020504, whole genome shotgun sequence.
ACCESSION   DAOQWR010000084 DAOQWR010000000
VERSION     DAOQWR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6436_length_10931_cov_6.020504"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKK36_07670"
     CDS             <1..240
                     /locus_tag="PKK36_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94471.1"
                     /translation="NSRFWAIFYCFWPFLSVFGHFLTFFGHFLAVFWFFYDFRGNIGR
                     NGGGYKEQDSARYRGWEHNFPENRRDHGGGVVAPL"
     gene            complement(527..4075)
                     /gene="dnaE"
                     /locus_tag="PKK36_07675"
     CDS             complement(527..4075)
                     /gene="dnaE"
                     /locus_tag="PKK36_07675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR94472.1"
                     /translation="MNKKPFIHLHFHTAYSLLDGACDVAKTMKRAAELNMDAVAMTDH
                     GVLYGTVEFYKAAKAAGIKPIIGCETYIAFGSRHERKHEAGKGYANHFVLLAETDEGY
                     DNLIRLISLSHLEGFYYKPRIDKELLSQYHKGLIGMSACLKGEVAECLSRGDMDEAVR
                     VAGEFSDIMGKDNFFLEMEDHGMPEQRLVNQGLVELAKRTGLPLVATNDVHYLEKEHS
                     EAHDIMICLQTQAVFSDPKRMRYSSRDLYMKSADEMWELFAEVPEALHNTRKIADRCN
                     VQLRLDTGELHFPQYKVPEGHSAEGWLIHLAKEGLKERYGIEDIKHPKDDEEKKVCAR
                     FQFEMGVIRQTGFINYFLVVSDFIRFARDQGIPVGPGRGSGAGSLVAYVLGITGLDPL
                     RYHLIFERFLNPERVSPPDFDIDFCQTRRGEVIEYVKEKYGRENVAQIITFGTLGAKT
                     VIRDIGRVLELPFADCDRLAKMVPEDVGTTLESAVANNPDFKRAIETEEFAKRIMKFA
                     RVLEGLPRNAGIHAAGVVIGDKPLIEILPLSLDKNKEPVTQFEMKPLEATGLLKMDFL
                     GLKTLTMIREAVDHIRVNHGVEVDVQNLPIDDQETFDLLNRGDTLGVFQVESRGMRDL
                     LRRIHLTQFEDLIAMIALYRPGPMNMLDPYVERKHGKVKITYDHPLLEEVLKETYGVM
                     IYQEQVQRAANVLAGFSLAQGDLLRRAMGKKQPEEMAKQRQKFIDGCMKANKIPAEKA
                     GKIFDNIERFAGYGFNKSHSAAYAIVSWQTAWLKAHYPAEFMAALLSIEIGNTDKLPI
                     YIAECREMDIEVRPPSVNESDVRFRPMKDGSIRFGMAGIKNAGTGAVEAIVRERQANG
                     PYKDLIDFCRRLDSRVVNRKVIESLVKCGAFDFSGMKRGRVFAGIDFAMSRAVAVQDD
                     LQRGQASLFDMLDEGGRGAVTDELPPADPWPQSTMLSAEKELLGFYISGHPLKEYEWV
                     LQKFSQNRVADLPSLETESGVHTRCGGLVCQFTRRFTKKKNEQQESRPMGTFHMEGLE
                     GAVDVVVFPRPFEQYGCLLADEAAVMVAGELRKKGEELVLQAKEIHALKDIPGLFSKC
                     MSLHIPSVNFDEKKAEQLKQVLRRHPGNTPVNICLQYPDGEKVFMDTTRSFRVTPCEK
                     LVNAVEHIIGEDAVYLDINPSTYLKEQKGRNGWNGF"
     gene            4256..6337
                     /gene="fusA"
                     /locus_tag="PKK36_07680"
     CDS             4256..6337
                     /gene="fusA"
                     /locus_tag="PKK36_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR94473.1"
                     /translation="MSRTVPISKVRNIGIMAHIDAGKTTVSERILFYTGKTHKLGEVH
                     DGAATMDFMVQERERGITITSAATSADWKGHQISLIDTPGHVDFTVEVERSLRVLDGA
                     IALFCAVGGVQPQSEKVWHQSEKYNVPKIAFINKMDRIGADFFGVVEQIQDELGANAV
                     PIMIPMGKEADFRGIIDLVTMKAIYYDEESQGRHFHEEDIPADKMEKAKKWRANLIEK
                     CAEQDDGLLEKFLEGQDFTEKDIWAILRKATIARRVVPVYCGSAFKNKGIQRLLDGVI
                     NCLPAPNEIPPIICEKGGTGREPNEEEPFSALAFKIVADKHMGKLIYIRIYSGTLHAG
                     TTVLNSTQEREQRVGRILRMHANRQENMEKAFAGDIVAIVGCAQTRTGDTLCDSQHPI
                     HLEAIEFPAPVISISIAPDSREDNEKLGTALHRLAEEDPTFTVGFDQETSETIISGMG
                     ELHLEIIVDRLKREFGVSATSGRPEVAYRETATDDAEGSYKYAKQSGGRGQFGHVVMK
                     IEPTPAGTGFEFVNAIKGGNIPAEYIPAVEKGVIQAMLAGSYAGYPVVDMCVTLLDGS
                     YHEVDSSDLAFQIAGRTCFKELFRKAGPQLLEPVMSIEVTTPEEFMGPVTSSVCQRRG
                     RIESMNDVRGVKIVSGYVPLSEMFGYSNALRTLTQGRANFSMHFERYEAVPFAITEQI
                     IAKRREANKVQ"
     gene            6498..8102
                     /locus_tag="PKK36_07685"
     CDS             6498..8102
                     /locus_tag="PKK36_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94474.1"
                     /translation="MTGDANIPAAGGGDRKRATDVQELLRLMGMFINNANLYGPQHAL
                     TAKAGDQCYSYLVPLQQKYPRINFSFSEGKVLIDGAAVDLSNPFVRNVVNRLVSLKIS
                     GFSLIQGMPKMEFYKLLGLIISGAETLGDDLEHVKSEKVTYQRVTEQEVVVDKDALDG
                     AGELDEKINRVQQIMAILKGEVSPDSPEALEALRETASDADQLANLIMEAAAIRQATA
                     SVATGESLGDIVVGCLRRSFDGLMADPASATQKGKKSVKKTMLLLEKGILDRLHALSD
                     GSHGEFDDQVQSEVEEMVDEVEIDALTDEYMKKRLAIEKSEQRLMRYMKKKGPEELEA
                     AGLREKLADAGLNPEGWKELVVKSGTGGGGGGEGGGSGDIGMLSILLTQLTDLMESAE
                     ATPDKLDKKVEGIDKEVENIAAGTGSKITELAQKIENERENLQHPEKHTAEELKQNHA
                     DMMELLAEIVQELCQPVAATNCAVGMLLSGHIGEISDVQRHMLDVAHRCGERLDTLLS
                     RLVEIVGLPEGLTPDKESVYGQKAGA"
     gene            8120..9010
                     /locus_tag="PKK36_07690"
     CDS             8120..9010
                     /locus_tag="PKK36_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNR94475.1"
                     /translation="MPAWFIPVIAATFIYGAINFLYKVAAERQLHSDRIVNMVGAAVA
                     LLSLLTLTVAQRGNPFPVFTRGVFLYAFFNGLFFGLGALTKFAALRHAPAGTVFPLNR
                     LNNMMVMLAGFLFFGDRPSVVQAAGLALTILVIALVSLDRPDSSGGHNFPHRKRGVVC
                     ALLSATCTAASMTVGKLLADTQENRLAYICCSYFLVFLITSVMTTVRKGSLPRGGRTG
                     WMPVLVFGLMIGGLNYGGYFLVLKGFGMGPISLVQAILSMSMVIPIALSCVVYRERLS
                     PANKLAIVLAVISAALISSP"
     gene            complement(9012..9749)
                     /gene="rsmG"
                     /locus_tag="PKK36_07695"
     CDS             complement(9012..9749)
                     /gene="rsmG"
                     /locus_tag="PKK36_07695"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNR94476.1"
                     /translation="MTDIAKKSQRAFPSPDRFRALCRSAGFSLSGPQEEKLGRYLDLV
                     LEANRKFNLTAVRDREEAWVRHVYDSLLLLPHLAGAKSVVDIGSGAGLPGIPLAVMRG
                     DLRVTLLEATRKKADFLAVTASELGLSGIKVLCARAENAGHDPAHRGRYDAAVVRAVG
                     SVAELAELAMPFLKMNGILAALKGAAVEEDVRGAARALRILKARTEGIQMAGEGDAVS
                     WILTLRKTGPTPAAYPRLPGTPKKTPL"
     gene            9791..10456
                     /locus_tag="PKK36_07700"
     CDS             9791..10456
                     /locus_tag="PKK36_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94477.1"
                     /translation="MKEKNLAGIIAHQLRHHAPFTAAGSLAGILLMYLCFQIGLTRPA
                     VTRAFWLLHPAHVLLSAFVTTAMYRHVARSSFWKALVIGYLGSVGIGTLSDSLIPYAG
                     EWILSMEHHDAHIGFIEKWWLINPLALLGIAGGYLLPKTKLPHFGHVLLSTWASLFHM
                     MMAHGDQAPMTAGLAAGIAVFLFLAVWLPCCTSDIVFPLLFTGYIPPCSCPGQAHSHE
                     EKV"
     gene            10440..>10931
                     /locus_tag="PKK36_07705"
     CDS             10440..>10931
                     /locus_tag="PKK36_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR94478.1"
                     /translation="MKKKYKYLFGPVPSRRLGLSLGVDLVPHKTCSMNCIFCQLGNTA
                     RTTIERREYVPTEDVLAEISDWQAQGGQTDVITLSGGGEPTLHSRFGEVIDFVKAKTR
                     HRTVILSNGSLLHLPEVRREAARADIVKITLASWDEDSFRKIHRPPAEVTFARIIEGA
                     CTLR"
BASE COUNT         2193 a         3369 c         3158 g         2211 t
ORIGIN      
        1 aattctcgat tttgggccat tttttactgt ttttggccgt ttttaagcgt ttttggccat
       61 tttttgacgt tttttgggca ctttttggcc gttttttggt tcttctacga ttttcgtggg
      121 aacattgggc gaaatggagg tggctataaa gaacaggact cagccaggta caggggctgg
      181 gaacataatt tcccggagaa tcggcgtgat cacggcggcg gtgtagtagc gcctctgtga
      241 ggcgcattct gaaacatatg ccgctcgcag agcggctact acaccagacc ccctgcaaat
      301 aaaggcgttc ccggctgttg tgatcatgga acgacgaaca tgaaaagccg tctgtgaatt
      361 tgtgtgaaag cggcgtccag ccgccgcact ccaaagcgcc gcgaaggtgg cgcaacgttt
      421 atcatttaac gccagaagtt gatttggata tcaaagcctt ccacgataat tgacgaagaa
      481 cccgtttttg ggtcattttt tcctgccggg cccgcaggcg atttccctag aatccgttcc
      541 agccgttgcg gcctttttgt tccttgagat aggttgacgg gttgatgtcg agatagaccg
      601 cgtcctcgcc aatgatgtgc tcgaccgcgt tcaccagttt ttcgcaggga gtaacgcgga
      661 agctgcgggt tgtatccatg aacacttttt cgccgtcggg atactgcagg cagatgttta
      721 ccggcgtgtt tccggggtgc ctccgcagca cctgcttgag ctgctcggct tttttctcgt
      781 cgaaattgac agacggaata tgcagggaca tgcattttga gaaaaggccg gggatatcct
      841 tgagcgcatg gatctccttt gcctgaagaa caagctcctc ccccttcttg cgcagctcgc
      901 cggccaccat caccgcggct tcatcggcca gcaggcagcc atactgctca aacgggcggg
      961 ggaagaccac cacatccacg gcgccttcaa ggccctccat gtggaaggtt cccatgggcc
     1021 gggattcctg ctgttcgttt ttctttttcg tgaaacgccg ggtgaactgg cataccagcc
     1081 cgccgcagcg ggtgtgcacc ccgctctccg tttccagcga aggcaggtcg gccacgcggt
     1141 tctggctgaa cttctgaagc acccattcgt attccttgag cggatgaccg gagatgtaga
     1201 atcccagcag ctctttttcg gcggaaagca tcgtgctctg gggccaggga tcagccgggg
     1261 gaagttcgtc ggtcacggcg ccgcgccccc cctcgtccag catgtcaaac aggctggcct
     1321 gcccgcgctg cagatcatcc tgcacggcaa cggcgcgact catggcaaaa tcaatccccg
     1381 cgaagacgcg gccgcgcttc atgccggaga agtcaaacgc gccgcacttc accaggcttt
     1441 cgatcacctt ccggttcacc acgcgcgaat cgagacggcg gcagaaatca atcagatcct
     1501 tgtaagggcc gtttgcctgc cgctcgcgca cgatcgcctc caccgcgccg gtgcccgcat
     1561 tcttgatgcc cgccatgccg aagcggatgc tgccgtcctt catcgggcga aagcgcacgt
     1621 cgctctcgtt cacgctgggc ggccgcactt cgatatccat ctcgcggcac tcggcaatat
     1681 aaatgggaag cttgtcggtg ttgccgattt cgatggacag cagggccgcc atgaattcag
     1741 ccggataatg cgccttgagc caggcggtct gccaggacac gatggcatag gcggcgctgt
     1801 gggatttatt gaagccgtag cccgcgaacc gctcgatgtt gtcgaatatt tttccggcct
     1861 tctcggcggg aattttattg gccttcatgc agccgtctat gaatttctgg cgctgcttgg
     1921 ccatttcctc gggctgcttc ttgcccatgg cgcggcgcag caggtcgccc tgcgccagcg
     1981 aaaagccggc caggacattg gcggcgcgct ggacctgctc ctggtagatc atgacgccat
     2041 aggtttcctt caggacttct tccagcaggg gatgatcata ggtgatcttc accttcccgt
     2101 gcttgcgctc aacgtaggga tccagcatgt tcattggccc cggccggtac agcgcgatca
     2161 tggcaatgag atcttcaaac tgcgtcaggt gaatcctccg gaggaggtcg cgcatgccgc
     2221 ggctttccac ctgaaacacg ccgagggtgt cgccgcggtt gagcaggtcg aaggtctcct
     2281 gatcgtctat cggcaggttc tggacatcca cttctacgcc gtgattgacg cggatatggt
     2341 ccacggcctc gcggatcatc gtcagggtct tgagtccgag gaagtccatc ttcagcaggc
     2401 ccgtcgcctc aagcggcttc atttcgaact gggtaaccgg ctccttgttc ttgtccaggc
     2461 tcagcgggag aatttcaatg agcggcttgt cgccaatcac cacgccggcg gcatggatgc
     2521 cggcattgcg cggcaggccc tcgagcacac gcgcgaactt catgatcctc ttcgcaaact
     2581 cctccgtctc gatggcccgc ttgaaatccg ggttgttcgc gacggccgat tccagcgttg
     2641 tgccgacatc ctccggcacc atcttggcca gccggtcgca gtcggcaaaa ggcagctcca
     2701 gcacacggcc gatatcgcgg atgacggtct tcgcgccgag ggtgccgaac gtgatgatct
     2761 gggccacgtt ctcgcggccg tatttttcct tcacatattc gatcacctcg ccgcggcggg
     2821 tctggcagaa gtctatgtcg aagtccggcg gcgacacgcg ctcgggattg aggaagcgct
     2881 cgaagatcag gtgatagcgc agcggatcaa ggccggtgat gcccagcacg taggccacga
     2941 gactgcccgc gcccgacccg cgcccggggc cgaccggaat gccctgatcc cgggcaaagc
     3001 ggatgaaatc gctcacgacc agaaagtaat tgatgaaacc ggtctgccgg atcacgccca
     3061 tctcgaactg gaagcgggcg cagaccttct tttcctcgtc atccttcggg tgcttgatgt
     3121 cctcgattcc gtagcgctcc ttgaggccct ctttcgcaag atgaatgagc cagccctcgg
     3181 cggagtggcc ttcgggaacc ttgtactggg gaaaatgaag ttcgcccgtg tccagtcgca
     3241 gctgaacatt gcagcggtcg gcgattttcc ttgtgttgtg cagggcctcc ggcacctcgg
     3301 cgaaaagctc ccacatctcg tccgcgcttt tcatgtagag gtcccggctg gaatagcgca
     3361 tgcgcttcgg atcgctgaaa acggcctgcg tctgcaggca gatcatgatg tcgtgcgcct
     3421 cgctgtgctc cttctcgaga tagtggacat cattggtggc caccagcggc aggccggtgc
     3481 gcttcgccag ctccacgagc ccctggttca ccagccgctg ctccggcatc ccgtgatcct
     3541 ccatttcaag gaagaaatta tccttgccca tgatgtcgct gaactcgccg gccacccgga
     3601 cggcctcgtc catgtcgccg cgggaaaggc actcggccac ttcgcccttg agacaggcgg
     3661 acatgccgat cagccccttg tgatactggc tcaggagctc cttgtctatc cggggcttgt
     3721 agtaaaaccc ctccagatgg gagagggaaa tcaggcggat caggttgtca tagccctcgt
     3781 cggtttcggc cagcagcaca aagtggttgg cgtagccctt tccggcctca tgcttgcgtt
     3841 catgccggct gccgaaggcg atataggtct cgcacccgat gatcggcttg atgccggcgg
     3901 cctttgccgc cttgtagaat tccaccgttc cgtagagcac cccgtgatcg gtcatggcca
     3961 cggcgtccat gttcagctcc gcggcgcgct tcatggtctt cgcgacatcg cacgcgccgt
     4021 ccagcaggct gtaggccgtg tgaaagtgca aatgaataaa cggtttcttg ttcacagtta
     4081 aaatccaaag acggcgggca gccccgtcag aaagttttat atcccaaccc gggcgccgaa
     4141 gcaaacggaa gcggcaaata atttcccgcg gccgaagatt tttttcattt cccccttgcg
     4201 aacggcggcc ggggagtgca tagtccggcg tttttctaat ggaaattgag gcaaaatgag
     4261 cagaacagta cccatttcaa aggtcagaaa catcgggatc atggcgcata tcgacgcggg
     4321 caaaaccacc gtgagcgagc gcatcctgtt ttacacggga aaaacccaca agctgggcga
     4381 agtgcacgat ggcgcggcca ccatggactt catggtgcag gagcgcgaac gcggcatcac
     4441 catcacctcc gccgccactt ccgcggactg gaaagggcac cagatcagcc tgatcgacac
     4501 tccgggccat gtggacttca ccgtcgaagt ggagcgcagc ctgcgggtgc tggacggcgc
     4561 catcgcgctg ttctgcgcgg tcggcggggt gcagccccag tccgaaaagg tctggcatca
     4621 gagcgagaaa tacaacgtgc ccaagatcgc cttcatcaac aagatggacc gcatcggcgc
     4681 ggactttttc ggggtggtcg aacagatcca ggacgagctc ggcgccaatg ccgtgcccat
     4741 catgattccc atgggcaaag aagcggattt ccggggcatc attgacctcg tgaccatgaa
     4801 ggccatctac tatgacgagg aaagccaggg ccgccacttc cacgaggagg acattccggc
     4861 cgacaagatg gaaaaagcca aaaaatggcg ggccaacctc attgagaagt gcgccgaaca
     4921 ggatgacggc ctgctggaga aatttctaga ggggcaggat ttcaccgaaa aggacatctg
     4981 ggccatcctg cgcaaggcga ccatcgcccg acgcgtggtg cccgtctatt gcggatccgc
     5041 cttcaaaaac aagggcattc agcggctgct ggacggcgtc atcaactgcc ttccggcgcc
     5101 gaatgaaatc ccgcccatca tctgcgaaaa gggcggcacg ggccgcgagc cgaacgagga
     5161 ggagcctttt tcggcgctgg cgttcaaaat cgtcgccgac aagcacatgg gcaagctgat
     5221 ctatatccgc atctattccg gaacccttca cgccggcacc acggtgctta attccacaca
     5281 ggagcgcgaa cagcgggtcg gccgcattct gcgcatgcac gccaaccgcc aggaaaacat
     5341 ggaaaaagcc tttgcgggcg acattgtcgc catcgtcggc tgcgcccaga cccggaccgg
     5401 cgacacgctc tgcgattcgc agcatccgat ccatctggag gccatcgagt ttccggcccc
     5461 cgtcatctcc atcagcatcg cgcccgactc gcgcgaggac aacgagaagc tcggcaccgc
     5521 cctgcaccgc cttgcggagg aggatcccac cttcaccgtg ggctttgacc aggagaccag
     5581 cgaaaccatc atctccggaa tgggcgagct gcacctggaa atcattgtgg accgcctgaa
     5641 acgggaattc ggggtttccg cgacctccgg ccggccggaa gtggcctatc gcgaaaccgc
     5701 aacggacgat gccgaagggt cttacaagta cgccaagcag tccggcggac gcggccagtt
     5761 tggccatgtg gtcatgaaaa tcgagccgac gccggccggt accggcttcg aattcgtcaa
     5821 cgccatcaag ggcggcaata ttccggccga atacattccg gcggtggaaa agggcgtcat
     5881 ccaggccatg ctggccggtt cctacgcggg ctatcctgtc gtggacatgt gcgtcaccct
     5941 gctcgacgga tcctatcatg aagtggactc cagcgacctg gccttccaga tcgccggccg
     6001 cacctgcttc aaggaactgt tccgcaaggc gggcccccag ctgcttgagc cggtcatgtc
     6061 catcgaagtg accacgcccg aggaattcat gggcccggtc accagctccg tctgccagcg
     6121 gcgcgggcgc atcgaatcca tgaacgatgt gcgcggcgtc aagatcgtca gcggatatgt
     6181 tccgctcagc gaaatgttcg gctattccaa cgcgctgcgc accctcacgc agggccgcgc
     6241 gaatttctcg atgcactttg agcgctacga ggccgtgccc ttcgccatca ccgagcagat
     6301 catcgccaag cgccgcgagg ccaacaaggt tcagtaaaag ctccaatccc tggaagtttc
     6361 tgcggaaaaa gttccaatca ttggaagttt tcggatgaaa agttccagtc attggaactt
     6421 ttcacttttt tgcatttcgc gcttaacttg gctccctttg tgctttagaa tgtcctgcaa
     6481 aaggatgtat cggagacatg actggagacg caaacattcc cgcagccgga ggcggtgacc
     6541 gcaaacgggc gaccgatgtg caggagctgc tgcgcctgat gggcatgttc atcaacaacg
     6601 ccaacctgta cggcccgcag cacgccctga ccgccaaggc cggcgaccag tgctacagtt
     6661 accttgtccc cctgcagcag aagtatcccc ggatcaactt cagcttctcc gaaggaaagg
     6721 tgctgattga cggcgcggcc gtggatctct cgaacccctt cgttcggaat gtggtcaacc
     6781 ggctggtttc gctcaagatt tccggttttt ccctgattca gggcatgccc aagatggagt
     6841 tctacaagct gctcggactg attatctccg gcgcggagac cctcggcgac gaccttgaac
     6901 acgtcaaaag cgaaaaagtc acatatcagc gcgtgaccga acaggaagtg gtggtggaca
     6961 aggatgccct ggacggcgcc ggggaactgg atgagaaaat caaccgcgtc cagcagatca
     7021 tggccattct gaaaggggaa gtttcgccgg attcaccgga ggcgctcgaa gcgcttcgcg
     7081 agaccgccag cgatgccgac cagctggcca acctgatcat ggaggccgcc gccatccggc
     7141 aggccaccgc ttcggtggcc acgggggaat ccctcggaga catcgtggtc ggatgcctcc
     7201 ggcgcagttt tgacgggctg atggccgatc cggcttccgc cacccagaag ggcaaaaaaa
     7261 gcgtcaaaaa aaccatgctg ctgctggaaa aaggcatcct cgaccgactg catgcgctgt
     7321 ccgatggaag tcatggggaa ttcgacgacc aggtgcagtc cgaagtcgag gagatggtgg
     7381 acgaggtcga aatagacgcc ctgaccgacg agtacatgaa gaagcgcctc gccatagaaa
     7441 aaagcgagca gcggctcatg cggtacatga agaaaaaagg tcctgaagaa cttgaagcgg
     7501 cgggactgcg cgaaaagctt gccgacgccg gcctcaatcc ggaaggctgg aaagagcttg
     7561 tggtcaagag cggaaccggt ggaggaggcg ggggcgaagg gggcggaagc ggagatatcg
     7621 gcatgctgag catcctgctg acccagctga ccgatctgat ggagtccgcc gaagccacgc
     7681 ccgacaagct ggacaaaaag gttgagggca tcgacaagga agtcgaaaac atcgccgccg
     7741 gcaccggaag caaaatcacg gaactcgcgc agaagattga gaacgagcgc gagaacctcc
     7801 agcatccgga aaaacatacc gctgaagaac tgaaacagaa ccatgccgac atgatggaac
     7861 tccttgctga aattgttcag gagctctgtc agcccgtggc ggccaccaac tgcgccgtgg
     7921 gcatgctcct ctccggccac attggagaaa tatctgatgt gcagaggcac atgcttgacg
     7981 tcgcccaccg ctgcggcgag cggctcgaca ccctgctttc gcgcctggtc gagatcgtcg
     8041 ggctgcctga aggcctgact ccggacaagg aatccgtcta cggccaaaaa gccggcgcct
     8101 gaccgccggt caacccatca tgcccgcatg gttcataccc gtcatcgccg cgacctttat
     8161 ttacggcgcc atcaattttc tctacaaggt tgcggctgaa cgccagcttc attccgaccg
     8221 catcgtcaac atggtgggcg ccgccgtcgc cctgctgtcg ctgctgacac tgaccgtcgc
     8281 gcagcgggga aatccctttc ccgtgttcac gcgcggcgtt ttcctgtatg ccttttttaa
     8341 cggccttttc ttcggtctcg gcgcgctcac caagtttgcg gcgctccggc acgcgccggc
     8401 cggcacggtc ttcccactga accgcctcaa caacatgatg gtcatgctcg cgggcttcct
     8461 gtttttcggc gaccggccgt ccgtggtgca ggcggctgga ctggccctga ccatcttggt
     8521 catagcactg gtctcgctcg accgcccgga ctcatcgggc ggacacaatt tcccgcacag
     8581 aaaaagaggc gtcgtttgtg cactcctcag cgcgacctgc accgctgcat ccatgaccgt
     8641 cggaaaactt ctggctgata cacaggaaaa ccgtctggcc tatatctgct gctcctattt
     8701 tcttgtattc ctgatcacct cggtcatgac cacggtccga aaaggctcgc ttccgcgggg
     8761 cggacgaacc ggatggatgc cggttctcgt cttcggactg atgatcggcg ggctgaatta
     8821 cggaggatat tttctcgtgc tgaagggatt cggcatgggc cccatctctc tggtgcaggc
     8881 cattctcagc atgtccatgg tgatcccgat cgcgctttcg tgtgttgtct atcgggagcg
     8941 gctcagtccg gccaataaac tcgcgatcgt gctggccgtg atttccgccg cgctcatcag
     9001 cagcccatga ttcacagcgg ggttttcttt ggcgtgcccg gaagacgcgg ataggcggcc
     9061 ggcgtcgggc cggtctttct gagcgtcaga atccacgaca ctgcatcgcc ctcgcccgcc
     9121 atctgaatgc cctctgtccg ggccttcaga atacgcagtg cgcgcgccgc gccgcggaca
     9181 tcctcctcca cggccgcccc cttcagggcc gccagaatgc cgttcatctt caggaagggc
     9241 atggccagtt ccgccagttc cgccacggaa ccgacggcgc gcaccacggc ggcgtcatac
     9301 cggccccggt gcgcggggtc atgccccgca ttctcagccc gggcgcacag caccttgatt
     9361 ccggacagcc ccagttcgct cgcggtcacg gccaggaaat ccgccttctt gcgcgtggct
     9421 tcaagcagcg tgacgcgcag gtccccccgc atcacggcca gcggaatccc cggcaggccc
     9481 gcgccgctcc cgatgtcaac gacgcttttt gcccccgcca gatgcggcag gagcaaaagg
     9541 ctgtcataca catgcctcac ccaggcctct tccctatcac ggactgcggt caggttgaac
     9601 ttcctgtttg cttcaagcac caggtccaga tagcggccca gtttctcttc ctgcgggccg
     9661 ctgagactga atccggcgga ccggcacagc gcccgaaagc ggtccggcga cggaaaagcg
     9721 cgttgtgatt tttttgcgat atctgtcacg cattcttgat attctttggc ggatgaatag
     9781 tctacacaac atgaaagaaa aaaatctggc cgggattatt gcacatcaac tccgtcacca
     9841 tgcgccgttc acagctgcgg gctcgctggc gggcattctg ctgatgtatc tgtgctttca
     9901 gatcgggctc acgaggcccg ctgtcacgcg cgccttctgg ctccttcatc cggcgcacgt
     9961 gctgctcagc gcattcgtca ccaccgccat gtacaggcat gtggcgcgat ccagcttctg
    10021 gaaagcgctg gtgatcggct atctcgggtc cgtgggcatc ggcaccctca gcgattccct
    10081 gatcccgtat gccggcgaat ggattctgag catggagcat cacgacgcgc acatcgggtt
    10141 catcgagaag tggtggctga tcaaccctct cgccctgctc ggaatcgccg gcggttatct
    10201 gctccccaaa accaaacttc cgcatttcgg gcacgtcctg ctcagcacgt gggcctcgct
    10261 tttccatatg atgatggctc acggcgacca ggcccccatg acggcgggcc ttgccgccgg
    10321 cattgccgtc tttctgtttc tcgcggtctg gcttccctgc tgcaccagcg acatcgtgtt
    10381 ccccctgctg ttcaccggat atatcccgcc ctgcagctgc ccgggacagg cgcattcaca
    10441 tgaagaaaaa gtataaatat ctctttggcc ccgttccctc gcgccggctc ggcctttcgc
    10501 tcggcgtgga cctcgtgccg cacaagacct gctcgatgaa ctgcatcttc tgccagctcg
    10561 gaaacaccgc gcgcacaacc attgagcggc gcgagtatgt cccgactgaa gacgtgctgg
    10621 ccgagatcag cgactggcag gcgcagggcg gacaaaccga cgtcatcacg ctttccggcg
    10681 gaggcgagcc gacactgcac agccggttcg gcgaggtgat agatttcgtg aaggcgaaaa
    10741 cccggcaccg gaccgtcatc ctgtccaacg gaagcctgct ccacctgccc gaagtccgcc
    10801 gcgaagccgc gcgcgcggac atcgtcaaaa tcacgcttgc ttcgtgggac gaggattcct
    10861 tccgcaagat tcaccggccc ccggcggaag tcacattcgc gcgcatcatc gagggcgcct
    10921 gcacgctccg g
//
LOCUS       DAOQWR010000085        10923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6444_length_10923_cov_4.780180, whole genome shotgun sequence.
ACCESSION   DAOQWR010000085 DAOQWR010000000
VERSION     DAOQWR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6444_length_10923_cov_4.780180"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKK36_07710"
     CDS             <1..192
                     /locus_tag="PKK36_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNR94479.1"
                     /translation="IQDLYGLEASPEIAGGRQKVSIEVLAPNRRPQQVTDDMRSFWTE
                     TYPILKKELRGRYPRHEWR"
     gene            complement(196..1524)
                     /locus_tag="PKK36_07715"
     CDS             complement(196..1524)
                     /locus_tag="PKK36_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNR94480.1"
                     /translation="MHSAFFAFYFTAMQMPSILKILAVFTAMVALTRTRIHLGLLLIL
                     GGIAMSAAAGLPFSETAGHLAEAAARSDLWLLIFITALIVIFGHLISEKETSAELMAA
                     VRGWGGRHGRAVSLMVVPAVIGLIPMPGGALFSAPLVAQTVGQESRRTGWKSAVNYWF
                     RHVWEHWWPLYPVTMITLSIFPIEIWQYTAAQFPMTLACLLFGWLLLVRPHLDSLADA
                     QAAPSTGNRTAGRVALALLATVLLAVFLPSVLHHLPVSTKPTTRKMASVAIGLAAGLL
                     VLTRGSFRALRARFRLALEIKALGIYLIIAGVVVFHHMLETSGLLPGAAAELVESSIP
                     LTLITILLPFLAGMVTGIAMGFAGTSFPLVAGLLAAPGSGMHPLSTAVLAFSSGYAGM
                     MLSPLHLCLILTRKYFSEGSAGVYRHIIPCATAVWLAAFAMFLVLRSLGL"
     gene            1585..1842
                     /locus_tag="PKK36_07720"
     CDS             1585..1842
                     /locus_tag="PKK36_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94481.1"
                     /translation="MNESSGSNIYNYGSRRDEEKMPEYRTSRKSRRRRQKKSRVDAAA
                     RFILYLLSAAVVIGLAVFVAVRLTKAGNARPEASAPSEPAP"
     gene            2060..2133
                     /locus_tag="PKK36_07725"
     tRNA            2060..2133
                     /locus_tag="PKK36_07725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2092..2094,aa:Gln,seq:ttg)
     gene            2179..3108
                     /locus_tag="PKK36_07730"
     CDS             2179..3108
                     /locus_tag="PKK36_07730"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNR94482.1"
                     /translation="MKIFSGNAHPGLAEKIAAYCGIPLGGAVVGRFPDGEIFVKYTES
                     LRGQDVFLIQPTALDPNEYLMELLIMTDAARRASAARITAVLPFYAYARQDRKDQPRV
                     PVTAKLVANLLVSAGVDRVLALDLHSHQIQGFFDIPLDHLRAAPAILKYINSLPLRNL
                     VVVSPDPGGLKMAINYADALGAGVAIGAKQRKSATEVEMTSLVGEVMDCDCLLVDDMT
                     TTAGTLCEAALLLKKKGANNIYAATSHALLTEQGVQRLKVSKIKELITTDSVPQIHTD
                     ADRVRVVSVAELIGEGIRRIHDNKSVSTLFELK"
     gene            3123..3824
                     /locus_tag="PKK36_07735"
     CDS             3123..3824
                     /locus_tag="PKK36_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNR94483.1"
                     /translation="MATAIKMTASKRAAQGSGPSRRLRKTGVVPGIVYGGGKDPVMVE
                     INEHDFKQMLRKHTSESLLMDLDIDGSENKVILKEIQHHPVTGRIQHADFRVVSMTEK
                     LEVSVPVVLVGEPVGVSLQGGVLESLMRSLTIRCLPADIPEKVEIDVSGLELGHHVAV
                     KDIKLDPAKFTMVTPADYAVAAVSAPRVQVEETPAEEAAAEGEAAGAEGAAAAPGAEG
                     AAPEKKEEKKEKKKE"
     gene            3856..4419
                     /gene="pth"
                     /locus_tag="PKK36_07740"
     CDS             3856..4419
                     /gene="pth"
                     /locus_tag="PKK36_07740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNR94484.1"
                     /translation="MKMVVGLGNPGPRYAGTRHNIGFRVLDRLAEQLGFDFRKSWFRN
                     AHYGKVRIGDEEVIMLKPQTFMNASGRAVRGEMRRRKSAPGELLVVLDDIEIPFGSLR
                     LRLKGGPGTHNGLKSVIEHAGSEDIPRVRVGVGRDPQAEDLAAYVLSDFPPETADDVE
                     RLVNRAADAVKCFLLEGPERAMNIFNS"
     gene            4446..4745
                     /gene="rpsF"
                     /locus_tag="PKK36_07745"
     CDS             4446..4745
                     /gene="rpsF"
                     /locus_tag="PKK36_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNR94485.1"
                     /translation="MSKYEGMFIFPKALSDEALEAVISSVGTEIEKLGGAVKSSTRLG
                     RRAFARPLKKQDSGHYAVIVFELAGRSLPALKARFKLMDGIMRVQIVSAPAVKEA"
     gene            4762..5187
                     /locus_tag="PKK36_07750"
     CDS             4762..5187
                     /locus_tag="PKK36_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR94486.1"
                     /translation="MASFNKVILVGNLTKDPEVRYTSSGTTVGNMRLAVTEHYKKQTG
                     EMQETTCFVDVEVWGRQAETCSEYLKKGSSLLLEGKLKLDEWETSEGEKRSKLRVRAD
                     RVQFLGSPRGDRSSGGSKPASSDESAMPADGGDDDNLPF"
     gene            5251..5463
                     /gene="rpsR"
                     /locus_tag="PKK36_07755"
     CDS             5251..5463
                     /gene="rpsR"
                     /locus_tag="PKK36_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003219224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNR94487.1"
                     /translation="MAGGEAGSRRRQGKLLQGMATIDYKDSELLKKFMTEHGKILPGR
                     TTGVSAKQQRQLKRAIRRARVMGLLA"
     gene            5507..5935
                     /gene="rplI"
                     /locus_tag="PKK36_07760"
     CDS             5507..5935
                     /gene="rplI"
                     /locus_tag="PKK36_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNR94488.1"
                     /translation="MTDIEGLGSEGSIVKVADGYARNYLLPLQKAAPVTDATRRRLAK
                     KRAEREEQMKQAAEAATKLANRIRNCSCTIAVKTGPEGKLYGSVGAADVLASLKEQGV
                     ELEKGQLEMPDSIRELGAYTIGVKLDAGVTSEFKVWIVEE"
     gene            5941..7368
                     /gene="dnaB"
                     /locus_tag="PKK36_07765"
     CDS             5941..7368
                     /gene="dnaB"
                     /locus_tag="PKK36_07765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNR94489.1"
                     /translation="MAGKTAKSAAPKSDRVPPHSEEAEKSILGSIFLDSDRVIDLCMT
                     RGLVPESFYEPRHRSLFEVFVQMNRENRSIDMVTVRDRLETLGRLEDVGGYDYIERLV
                     DSTPTAIHAEHYADIVFQKYLLRRIIDRSREAIDRCYESDEDAPIVLNKTEQAILEIS
                     QTQHQPLEQWDKLVEGVMKEIMRSYETKTGLTGIPSGYKDIDDKLQGFKPGELVILAA
                     RPSQGKTSLALNIAENLAMPTHTDPDPRPVAVFSLEMSSESLVRRMICSRARVSASKI
                     TGGYISRAHNDALTNAATALLKAPIYLDDTAALEALEVRSRARRLKRNYDVQMIIVDY
                     LQIMNFAQYSKEGRQRETSAISATMKAMAKELKVPVLVLSQLSRNPENRTGPSAAPKL
                     SDLRDSGSIEQDADVVLMLRRPSSYPEDKDHADEKLAIVNVAKNRNGPTGDIKLNFFA
                     DYTRFETRYEGVDEPGAAVTYEDEE"
     gene            7382..9649
                     /gene="bamA"
                     /locus_tag="PKK36_07770"
     CDS             7382..9649
                     /gene="bamA"
                     /locus_tag="PKK36_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNR94490.1"
                     /translation="MQLISAGVAVFWGLMSLAFAEDVSEIRVRNTGIGPLEEQSVMAF
                     TSLKAGEPFAQARVSRDVRMLQNSGRFSDVQVQVERAAGGLIVTYAVNPRLRINRIAV
                     EGADYIGNKKALELLDLGPGDLVDDVLLGTRMQPVRDHYAKRYFPDVNLSWDIVPRAE
                     TGLCDVTVRVHEGQRAKVKRIRFRGNRAVKARDLRGVMQQRRVRLWSWFTNSGRYNPD
                     ELAMDVGSLRSVYLDRGYLDAVVGEPVVHRARRKVAIEIPVQEGRKYTVGEIAIDGME
                     LFPEAAVRPFLPLKRGADASMRAIRQTADVIRDFYGRKGYIETRVTYDIRTAPDGTAD
                     LAFSVREGTLARARDVLIRGNTRTKDKVIRRELTIMPGQILNEVAVRVSKSRLQNLGW
                     FSFAESALVPTMDPEYYDVVFEVEEMRTGQFLVGAGFSSIDKVSGFVEISQGNFDLFH
                     WPFTGGGQKAKFRAQLGTERTDMELSFVEPWFLDRKLALGIDLYQHERRFLSDDYDQE
                     NMGAAVSLSVAAGRFSRLKLRYAIEDISVENVSDDASELIRAEEGSRLKSSMTLSLSR
                     STLNSSFVPTRGNLSSLSGTLAGGPLGADTDLYELEARTSQYVPMLFGHYLNLRGTAT
                     VVEEYGDAESVPLFDRLFLGGAHTIRGFQYRDVGPKDETGEPIGGRTAAYVSAEYNVP
                     VVKNLRLVTFYDMGMVWEEAYDFSGDFNSAWGIGVRVDIPGFPLQFDYAWPIEADEWN
                     DQPSGRFSFLIGYKL"
     gene            9667..10302
                     /locus_tag="PKK36_07775"
     CDS             9667..10302
                     /locus_tag="PKK36_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNR94491.1"
                     /translation="MKKHLIPALAAAVMMAAPALRAADQMVFVNLNRVFEEYHKTEMA
                     NKQLKAQADEFSAERSGKIKEFEELQAAFDAARDDSQNPALSAEARDAKRDEAEEKLI
                     EIRRKEKEIRKYEETRKKQLMDTERRVRNRIVGEIRDAIKAYAIEQAYSAVIDSSGQS
                     LNAVESIVYHDPRLEITDQILEILNKGAEQAAPIELDAPDAEEEEEPEAVE"
     gene            10299..>10923
                     /locus_tag="PKK36_07780"
     CDS             10299..>10923
                     /locus_tag="PKK36_07780"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNR94492.1"
                     /translation="MKRTVREIAAAVGGELCGDPDVEICAVASLREAGPGDITFIANP
                     RYAGLVAETRASAVLVGRSWTDSSAAVLIRVDDPDRAFTQTAEFFAPPPITPPPGIHP
                     TAVIASDARIGEGASIGPCCIIEPGVQIGARAVIFGGCCIGHEVTIGDDLRMYPHVSV
                     RERCRIGHRVILHNGAVIGSDGFGYNVDKNGVRTKIPQTGIVTIGDDV"
BASE COUNT         2434 a         2916 c         3487 g         2086 t
ORIGIN      
        1 attcaggacc tgtacggcct ggaggccagt cccgagatcg cgggcggccg gcagaaggtg
       61 agcattgagg tgctggcgcc gaaccggagg ccgcagcagg tcaccgacga catgcggtca
      121 ttctggacag agacatatcc gatcctcaaa aaagaactgc gcggccgtta tccccggcac
      181 gaatggcgct gatttctaca ggcccagact gcgcagaaca agaaacatcg cgaacgccgc
      241 gagccatacc gcggtcgcgc agggaatgat gtggcggtat acccccgcag agccttcaga
      301 gaaatatttc cgcgtgagaa tcaggcagag gtgcaggggc gagagcatca tgcccgcgta
      361 acctgacgag aaggccagca cggcggtgga aagaggatgc atcccgctgc ccggggccgc
      421 cagcagtccc gccacgaggg gaaatgaagt gcccgcgaag cccatggcaa ttcctgtaac
      481 cataccggcc agaaaaggaa gcaggatggt tatcagggtg aggggaatgc tgctttcaac
      541 cagttcggcc gccgcccctg ggagaaggcc gctggtttcc agcatgtgat gaaacaccac
      601 aactccggca atgatcaggt agatgcccag cgccttgatt tcaagcgcca gccggaagcg
      661 cgcgcgcagc gcgcgaaagg acccccgtgt caggaccagc agtcccgcag cgagtccgat
      721 ggccacggaa gccatcttgc gcgtggtggg tttcgtcgaa accggaagat gatgcaggac
      781 ggaaggaaga aacacggcca gcaggacggt ggccagcagg gcaagggcaa cccgtccggc
      841 tgtccggttt cccgttgaag gcgcggcctg cgcatcggcc aggctgtcca gatgcggccg
      901 caccagcagc agccagccaa acagcaggca ggccagcgtc atggggaact gggctgcggt
      961 atactgccaa atctcaatgg ggaagatcga gagggtgatc atggtgacgg gatacagggg
     1021 ccaccagtgt tcccacacat gccggaacca gtaattcaca gcggacttcc agccggttcg
     1081 ccggctttcc tgcccgaccg tctgtgcaac gagcggcgct gaaaaaagag cgcctccggg
     1141 catggggatc agtccaatca cggcggggac caccatcagg ctgacagcgc ggccatgccg
     1201 tccgccccag ccccggacgg cggccatcag ttcagccgat gtctcctttt cggaaatcag
     1261 gtgcccgaag atgacaatga gcgcggtgat gaaaattagc agccagaggt cagaacgcgc
     1321 cgcggcctcc gccagatgtc cggccgtttc gctgaacggg aggccggccg ccgcgctcat
     1381 ggcgattcct ccaaggatca gcagcagtcc aagatggatg cgggttcgtg tcagggccac
     1441 catggccgtg aacacggcca ggattttcag gatggaaggc atttgcatgg cggtgaagta
     1501 aaacgcaaaa aatgcggaat gcaaatgtta aatcttggca aaagcctgtt tcgtagtatt
     1561 ctccgcagct tgaatgagtg ttttatgaac gagtcatcgg ggagcaatat ctacaattac
     1621 ggctcgcgac gcgatgagga gaagatgccg gaataccgga cttcccggaa atcccgccga
     1681 cgccgtcaga aaaagagccg ggtggatgcg gcggcacggt ttattctcta tctgctttcc
     1741 gccgcggtcg tgatcggact tgcggttttt gttgcggtgc gcctgacaaa ggccgggaac
     1801 gcccggccgg aagcctccgc cccgtccgaa ccggccccat gatccctgcc tgaaggcatg
     1861 aaaatcctgc gtgccggccc gcccgtgaaa aactgcggat tgcttcaacc gggtcttgac
     1921 ttcccgggga caaggctcta gtgtcaggcg ctttgccaaa ggtaatgtga agaaagagtg
     1981 gctgagcggt ctgtgttccc ccggccggga agttgaggta tgtcctgttc cggcgccgcg
     2041 gcaacagatc agaaggatct gcccgatggt gtaatggcag cacacggccc tttggaggcc
     2101 agagtccagg ttcgagtcct ggtcgggcaa ccacaggatg cgaagaacag cactggatac
     2161 agagagcagg agtttgtttt gaagatattt tcaggaaatg cccatcccgg gctggcggag
     2221 aaaatcgccg catactgcgg catcccgctg ggcggagccg tcgttggacg gttcccggac
     2281 ggggagattt tcgtgaaata cacggaaagt ctccggggtc aggatgtgtt cctgattcag
     2341 ccgacggcgc tggatcccaa cgaatacctg atggaacttc tgattatgac ggatgcggcc
     2401 agaagggcct ccgccgcgcg catcacggcg gtgctcccgt tttatgccta tgcccgtcag
     2461 gaccgcaaag accagccccg ggttccggtg accgcgaaac tcgtggcgaa cctgctggta
     2521 tcagccggag tggaccgtgt tctggcgctg gatctgcatt cgcaccagat tcagggtttt
     2581 ttcgacattc cgctggatca cctgcgcgcg gcaccggcca tactgaaata catcaactcg
     2641 cttcctctcc gcaatctggt cgtggtttcg cccgatcccg gggggctgaa gatggctatt
     2701 aattatgcgg atgcccttgg agccggcgtg gcgatcgggg ccaagcagcg gaagtcggcc
     2761 acggaagtgg aaatgaccag ccttgttgga gaggtgatgg actgcgactg cctgctggtg
     2821 gatgacatga ccaccacggc cggaacactc tgcgaggccg ccctcctgct gaagaagaaa
     2881 ggcgccaaca atatttatgc cgccaccagt cacgccctgc tgaccgaaca gggggtgcag
     2941 cggctgaagg tttccaaaat caaggaactg atcacaacgg attctgttcc gcaaatccac
     3001 acggatgcgg acagggtgcg ggtggtttca gtggctgaac tgatcgggga aggcatccgg
     3061 agaattcacg acaacaaatc ggtgagcaca ctttttgaac tcaaataagg gagtaaacga
     3121 aaatggcgac agcaattaaa atgacagcat ccaaacgagc ggcacagggt tctggccctt
     3181 cccgccgcct gcgtaaaacg ggtgttgttc ccgggattgt atatggcgga ggcaaggacc
     3241 ccgtaatggt ggaaatcaat gagcatgatt tcaagcagat gcttcgcaag cacaccagcg
     3301 agtccctgct gatggatctg gacattgacg gcagtgaaaa caaggtgatt ctgaaagaga
     3361 ttcagcatca tccggtcact ggccgcattc agcatgcgga tttccgcgtg gtttcaatga
     3421 cagagaaact ggaggtctcc gtgccggtgg tgctcgtggg cgagccggtc ggcgtttccc
     3481 tgcagggcgg ggtgctggag agcctcatgc ggtcactgac cattcggtgt ctcccggccg
     3541 atattcctga aaaggtggaa attgatgtca gcgggctgga actcggtcat catgtggcgg
     3601 tgaaagacat caaactggat ccggcgaaat tcacaatggt gactccggcg gattacgccg
     3661 tggcggcggt gtccgctccc cgtgtccagg ttgaggaaac accggccgaa gaagccgccg
     3721 cggaaggtga agcggccggc gctgaaggcg ccgcggctgc tccgggcgcg gagggcgctg
     3781 ctcccgagaa gaaagaggag aagaaggaga agaagaaaga ataggtccgc cgggcggccg
     3841 gccggggaat acgacatgaa aatggtagtg ggacttggca atcccgggcc ccgctatgcc
     3901 ggaacccggc ataatatcgg tttccgggtg ctggaccggc tggccgaaca actgggcttc
     3961 gatttcagaa aaagctggtt tcggaatgcg cattacggca aggtgcgcat cggtgatgaa
     4021 gaagtgatca tgctcaagcc gcagacgttc atgaatgcca gcggaagggc cgtgcggggc
     4081 gaaatgcgcc ggaggaagtc ggcgcccggt gagctgctgg tggtgctcga tgatatcgaa
     4141 atccccttcg gttcgctccg tttgaggctg aagggcgggc cgggaacgca caacggactg
     4201 aagtcggtga ttgagcatgc cgggagcgag gatatccccc gggttcgtgt gggggttggc
     4261 cgggacccgc aggcggagga tctggcggcg tatgtgctgt cggacttccc gccggaaacg
     4321 gcagacgatg tggagcggct cgtgaaccgg gccgccgatg cggtgaagtg ctttttgctg
     4381 gaggggccgg agagggccat gaacattttt aacagttgac ggatcaaagt caggagaagt
     4441 cgcagatgag taagtacgaa ggcatgttta tttttccgaa ggccctgagc gatgaagcgc
     4501 ttgaggcggt tatcagttcc gtgggcacgg aaattgaaaa actgggcggc gccgtcaaga
     4561 gttccacccg gctgggccgc cgcgcattcg cgcgtccgct gaagaagcag gattcaggcc
     4621 attatgcggt gattgtcttt gagctggcgg gccgcagcct tcctgccctc aaagcgcgtt
     4681 ttaaactgat ggacggcatc atgcgggtgc agattgtttc cgcccccgct gtaaaagaag
     4741 cctgaaccgg gagcagcagc catggcatca ttcaataaag tcattctggt ggggaatctc
     4801 acgaaggatc cggaggtgcg ttatacatcc tcggggacca cggtgggcaa catgcgcctg
     4861 gccgtgaccg aacattataa aaagcagacc ggggaaatgc aggagacgac ctgcttcgtg
     4921 gatgtcgagg tttggggccg ccaggccgag acctgcagcg aatatctgaa gaagggatca
     4981 tcgcttctgc tggaaggcaa gctcaagctg gatgagtggg aaacgtctga aggtgaaaag
     5041 cggagcaaac tgcgggtgcg cgccgaccgc gttcagtttc tcgggtctcc gcgcggcgat
     5101 cgttcctccg gcgggtcgaa gccggcttcc tccgacgagt ccgccatgcc cgccgacggc
     5161 ggcgatgacg acaacctgcc attttaactg taaaaacaaa aggggtttga aataaagaat
     5221 atgaaaaaac agtatcagag caactgggac atggcgggcg gagaagcggg cagccgcagg
     5281 cggcagggca agctcctgca gggcatggcc accattgatt acaaggacag cgaactgctg
     5341 aagaagttca tgaccgagca tgggaaaatc ctgcccggcc gcacaacggg cgtttccgcc
     5401 aagcagcagc gccagcttaa acgcgccatc cgcagggcgc gagtgatggg cctcctggcc
     5461 tgatttttat tgaaaggttt cagtcatggt taaagaagtt attttaatga cggatataga
     5521 aggactcggc tcagaggggt ccattgtcaa agtggccgac ggctatgccc gcaattacct
     5581 cctgcctctt cagaaggctg cgccggtaac ggatgcgacc cgccgccgcc ttgccaagaa
     5641 acgcgcggag cgcgaagaac agatgaaaca ggccgcggag gccgccacca agctggccaa
     5701 tcgcatccgc aactgctcct gcacgattgc cgttaaaacc gggcctgaag gcaagctcta
     5761 cgggtcggtt ggcgcagccg atgttctggc ttccctgaag gagcagggtg ttgaactgga
     5821 aaaggggcag ctggaaatgc ccgactccat tcgcgagctc ggggcatata ccatcggcgt
     5881 taaactggat gccggtgtca cttcggagtt caaagtctgg atcgttgagg aataggattc
     5941 atggccggaa agaccgccaa gtctgccgcg cccaagtcag atcgtgtgcc tccgcacagc
     6001 gaggaggcgg agaagagcat cctcggctcc attttccttg attcagaccg ggtgattgac
     6061 ctctgcatga cccgggggct tgttccggaa agtttttacg agccgcgtca ccgaagcctt
     6121 ttcgaagtgt ttgtgcagat gaaccgggaa aaccggagca tcgacatggt cacggtcagg
     6181 gaccgccttg agacgctggg ccggctggag gatgtgggcg gctacgacta tatcgagcgg
     6241 ctggtggaca gcactccaac cgccatccac gcggagcact acgcggatat tgtgttccag
     6301 aagtacctgc tgcgccgcat catagaccgt tcccgcgagg ccatagaccg ctgctatgaa
     6361 tcggatgagg acgctccgat tgtcctcaat aaaaccgagc aggccattct cgaaatctcc
     6421 cagacccagc accagccgct ggaacagtgg gacaaactcg ttgaaggcgt catgaaagag
     6481 atcatgcgca gctacgagac caagaccggt ttgacgggca tcccctccgg atacaaggat
     6541 attgatgaca aactgcaggg gttcaagcca ggcgagctgg ttattctggc tgcccgcccg
     6601 tcgcagggaa agacgtcact ggccctgaac atcgcggaaa atctggcgat gccgacacat
     6661 accgatcccg acccgcggcc ggtcgcggtg ttcagcctgg aaatgtccag cgaatccctg
     6721 gttcggcgca tgatctgttc gcgcgcccgg gtatcggcga gcaagatcac gggcggttat
     6781 atcagccgcg cgcataatga tgcgctcacg aatgccgcca ctgcgctgct caaggcgcct
     6841 atatatctgg atgatacggc cgcgctggag gcgctggaag tgcgctcgcg tgcccgccgg
     6901 ctcaagcgca actatgacgt gcagatgatt attgtggatt atctgcagat catgaatttt
     6961 gcacagtatt cgaaagaggg gcgtcagcgt gagacttccg ccatttccgc cacaatgaag
     7021 gccatggcca aggagctgaa ggtcccggtg ctggtgctca gccagctgag ccgaaatcct
     7081 gaaaaccgca cgggcccgtc cgccgcgccc aagctttcgg acctgcgcga ttccgggtcc
     7141 attgagcagg atgcggatgt ggtgctcatg cttcggcgcc ccagcagcta tcccgaggac
     7201 aaagaccacg ccgatgaaaa actggctatt gtgaatgtcg ccaaaaacag gaatggtccg
     7261 accggtgata taaaactcaa tttctttgca gactacacac gctttgaaac cagatatgaa
     7321 ggggttgatg aacccggggc ggcagtcacc tatgaggatg aggagtaaac gcttgaaacg
     7381 catgcagctc atttcagccg gggtggcggt tttctgggga ctgatgtcac tggcgttcgc
     7441 ggaggatgtc agcgaaatcc gggttcggaa cacggggatt ggcccgcttg aagagcagtc
     7501 cgttatggcg ttcacctccc tgaaggccgg cgaaccgttt gcccaggcgc gggtttcccg
     7561 tgatgtgaga atgctgcaga acagcggccg cttttccgat gtgcaggtgc aggtggaacg
     7621 ggccgccggc ggccttattg tcacgtatgc ggtgaatccg cgtctgcgca ttaaccgcat
     7681 tgccgttgaa ggggcggact atatcggaaa caagaaggcc ctggaactgc tggatctcgg
     7741 cccgggtgat ctggtggacg atgtgctgct gggcacgcga atgcagcccg tgcgtgatca
     7801 ttacgccaaa agatatttcc ccgacgtcaa tttgagctgg gatatagttc cgcgtgcaga
     7861 gaccggcctt tgcgatgtga ccgtgcgggt gcatgagggc cagcgggcca aggtgaagcg
     7921 gattcgcttc cggggtaatc gcgccgtgaa ggcccgggac ctgcgagggg tgatgcagca
     7981 gcgccgtgtg cggctctggt catggttcac taattcgggc cgctataatc cggacgagct
     8041 ggcgatggat gtcggatccc tgcgcagcgt ttatctggat cggggttatc ttgatgcggt
     8101 ggtgggagag cccgtggtgc accgcgcgcg gcgcaaagtt gcaatcgaaa ttccggtcca
     8161 ggagggacgg aaatacactg tgggggaaat cgccattgac gggatggaac tctttccgga
     8221 agccgccgtg cggcccttcc tgccgctgaa gcggggcgcc gatgcctcca tgcgggccat
     8281 tcgtcagacc gccgatgtca tccgtgattt ctacgggcgc aaaggatata ttgaaacccg
     8341 cgtcacatac gacatccgga ctgctcctga cggcacggcc gatctggcat tcagtgtccg
     8401 ggagggcaca ctggcccggg cccgggatgt gctgatccgg gggaacaccc gcacgaagga
     8461 caaggtcatc cgccgggaac tgaccatcat gcccgggcag attctcaacg aagtcgccgt
     8521 tcgcgtcagc aagagccgcc tgcagaatct gggctggttt tctttcgcgg agagcgccct
     8581 ggtgcccacc atggatccgg aatactacga tgttgttttt gaggtcgaag aaatgagaac
     8641 cggacagttt ctggtcggcg cagggttctc gagcatcgac aaggtcagcg gtttcgtcga
     8701 aatatcccag ggaaattttg atttgttcca ctggcctttc acgggcggcg ggcagaaggc
     8761 caaattccgc gcccagctcg gcaccgaacg cacagacatg gagctttcgt ttgttgaacc
     8821 ctggtttctg gacaggaaac tcgccctcgg cattgatctc tatcagcacg agcgccgctt
     8881 cctcagcgat gattacgatc aggaaaacat gggggcggcc gtcagtctca gtgtggccgc
     8941 cggacgtttt tcgcggctga aactccgcta cgccattgag gatatttcgg tggaaaatgt
     9001 cagcgatgat gcatcagaac tgatacgggc ggaggagggc tcgcggctga aaagctccat
     9061 gacgctttcg ctctcgcgca gcacgctgaa cagcagtttt gtgccgaccc gggggaatct
     9121 gtcgagcctg tcaggaacgc tggcgggcgg accgctgggg gcggatacgg acctgtatga
     9181 actggaagcc aggacgtcgc agtatgttcc catgctgttc ggacactacc tgaatctgcg
     9241 aggaacggcc accgtggttg aggagtatgg agacgcggaa agtgttccgc tgttcgaccg
     9301 gcttttcctg gggggcgctc acaccatccg cggtttccag taccgagatg ttggaccaaa
     9361 agatgaaacc ggggagccaa tcggcgggcg gacggcggct tatgtgtcgg ccgagtacaa
     9421 tgtgcccgtg gtgaagaatc tgagactggt gaccttctat gacatgggca tggtctggga
     9481 ggaagcctat gatttcagcg gggatttcaa ttccgcctgg ggaattggcg tgcgcgtgga
     9541 tatccccgga ttccctctgc agtttgacta tgcctggccc attgaagcgg acgagtggaa
     9601 tgatcagccg tccggccgct tcagttttct tattggatat aaactctaac agaaaaggag
     9661 tcgctcatga aaaaacatct cattcctgca ctggccgccg cggtcatgat ggctgcgccc
     9721 gccctgcgtg ccgcagatca aatggtgttc gtgaatctga atcgggtgtt cgaagagtac
     9781 cataaaacag aaatggccaa taagcagctc aaggcccagg ccgacgaatt cagcgccgag
     9841 cggagcggga aaatcaagga gtttgaggaa ctgcaggcgg catttgacgc cgcccgtgat
     9901 gattcccaga atcccgccct ctctgcggaa gcccgtgacg ccaaacgcga tgaagctgaa
     9961 gagaaactca ttgagattcg ccgcaaggag aaggaaatcc ggaagtatga ggaaacacgg
    10021 aaaaagcagc ttatggacac ggaacgccgt gtgcgcaacc ggattgtggg ggagattcgc
    10081 gatgcgatca aggcttatgc gattgaacag gcttattccg cggtgattga ttcctcggga
    10141 cagtcgctga atgcggtcga aagtatcgta tatcatgatc cccgtctgga aatcaccgat
    10201 cagatactgg aaattctcaa caagggcgcg gaacaggctg ctccgattga gttggatgcg
    10261 cctgatgccg aggaagaaga ggaacctgag gcagtggaat gaaacggacc gtgcgcgaga
    10321 tagccgccgc agtcggcgga gagctctgcg gcgacccgga tgttgagatt tgcgcggtgg
    10381 ccagtctaag ggaagcaggg ccgggcgaca tcactttcat cgccaatccg cgatatgcgg
    10441 ggctggtggc ggaaacccgg gcatcggcag tgcttgtggg ccgttcatgg actgattctt
    10501 ccgccgccgt cctgatccgg gtggatgatc ccgacagggc ttttacgcag acggctgaat
    10561 tcttcgctcc tccacccatt acgccgcccc cgggcattca tccaacggca gtcattgctt
    10621 ctgatgcgag gatcggcgag ggggcaagca tcggaccctg ctgcatcatt gaaccgggtg
    10681 ttcagatcgg cgcgcgcgca gtgattttcg gcggctgctg cattggacat gaggtgacaa
    10741 ttggcgacga tcttcggatg tatccgcatg tttcggtccg tgagcgctgt cgcattgggc
    10801 atcgtgtgat tctgcataac ggggcggtga tcggcagtga tggctttgga tataacgtgg
    10861 acaaaaacgg agtccgcaca aaaatccctc agaccggaat tgtaacgatc ggcgatgatg
    10921 ttg
//
LOCUS       DAOQWR010000086        10870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6492_length_10870_cov_5.830883, whole genome shotgun sequence.
ACCESSION   DAOQWR010000086 DAOQWR010000000
VERSION     DAOQWR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6492_length_10870_cov_5.830883"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="PKK36_07785"
     CDS             complement(<1..1622)
                     /locus_tag="PKK36_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNR94493.1"
                     /translation="MAHRRDKELEEFRSVMEVPSSFEEGFNWTALIGAIFIALLMVPG
                     AIYMGLLAGVGNIGAASQWVTVILFIEVAKRAHKTMKKAEIFVLFFMAGAAMGMPFSG
                     LIWNQFFARSDAAIAAGIADQLPIWYAPPADSESYVARTFIHADWLPVIGLIIFGTFF
                     GQLANMVLGYGLFRLASDVERLPFPMAPIGAQGIIALAEDADEKNLESRASQWRWRVF
                     SIGGALGLLFGTVYLLLPTLTGALTGEPIQIFQIPFSDFTAKTGRYLPAVATGMSWDF
                     GNIIFGMVLPFFAMVGSFIGLIVTFILNPMLYHFGIIHNWTPGDSTINTLYKNNIDFH
                     FSFQIGIAMAIAIAGFWQVFRSFRNRKVQAETQEGPRSSVPEGRGDIRVWLVILVYFF
                     ITVTYILVSGWLIDWHRGIMIVLLLLGFVYTPIISYVTARLEGMVGQVVEVPYIREAA
                     LILSGYHGAACWFLPIPMANYGQMTVFYKQCELTGTRFPSIWKTQIVLFPIILVSSIF
                     FMNFIWGLNEVPSAVYPFAEMMWELEAENQCI"
     gene            complement(1612..6381)
                     /locus_tag="PKK36_07790"
     CDS             complement(1612..6381)
                     /locus_tag="PKK36_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNR94494.1"
                     /translation="MLKKLKMMAGLMPALALAAVADSGSFPADLEALTRGPHRLTGTM
                     EYRAAADHVKARLEQIGVDEVIVQEFPAAQTVTKRCELVFDGGQKIPLVPARPDGVIP
                     PVSSPEGITGELVWLGAGHAGDFERADPQGKIAVMDYNTDDQWLKAFRLGAKAVIFTR
                     QEPCESRHHHHVRANANLPRFYYDGPASNLTEGARVSLHSEIVWEKTTGRNVIGYLKG
                     TDPVFDLGKEEVIIISANLDSFGEIPERTPGARPAANCAALLQLAEMLRDQRPRRGAV
                     FLFADGQARSHAGARAFYEALEDKVKQADLDARMKAWKTESSFIRQVREQMKKDDPLA
                     DRISPSGKELLKRLRSAATDYAQEIRGQLMELRAKEKEDESADFSARIDELASAQNRW
                     NDLRRELGRGSVSENSQPLFREVLKTVHASVEARASELRAAKAMLDSSLKVRDLLADR
                     WIALHISLMLGDTTERWGVVIGGDADIHSAQDDPGLYGRIQGSFLRAWESLEKGKTRA
                     AHFEPASVDGRLTQTRLLWNAPMLIHGGEIAGRHGIYNIVLGTMHETLPREGTPDDTA
                     EFLNTDIIRQTTREIALLLAAVASLETQLGAVEAVADQEGLSLRRAIAADKQYITAGF
                     ENGRATGALVMGRQRGSSIPNRPVPGAVVQISLVDPWNNCFWPHNKPYAFDPFIVTMT
                     DENGAYSAGSLPEDEYSIFGFSASFDGSGRVVMTSENKSYRSVLTRLNMLNVNSGLLM
                     LPPSFWPQNASVMNARANAAISGDDSDKAYAETADGVVYWFAEEKLKGIKAFGLNSIV
                     ALINDRPVEGGAEMYGAGYPAGRTDWLSLKNSEQSAWDLWRLNEGRLAILRSRDILNS
                     SLEELHGRIRDSLDEAARSDSPVLKEALAASAFMSATPVYEMIRTSMDDLVKAVLVLL
                     ALCVPFAFALERLLIGSNMIYKQVGWFTGFFTLTFIILYLSHPAFAIAKTPVIIFLGF
                     AVVLLSVLVIFIIMRKFEAELKALQGMTASIHAADISRFNTVMAAMAMGISTMRRRPL
                     RTALTAITIILLTFTILCFASFDTRKGIMKLFSAPSPAYTGAVLRNPTWGSYNLEFVD
                     VIRSRWEKDAEVCTRFWICPEFARDPDFVVALADGSNPVTLKGMLGLSPQEIRRRDDL
                     KALLQITDGDDLDSHAFMTEAVAELLNVKPGDEVLLKGLRLKVGPLISASALSTARDM
                     DGSSILPVDFAQMKAAQKDQTVQSDDMTQEQDTWTSLPVDSIIITSAENTRRAGGKPH
                     LIAIYTEDAGQASDIAEEAARMQDKTPVLATRTDGVYRHILGTVMAASGVGDLFFPVL
                     LGGLVIFGTMLGSVADREKEIFTFSALGLAPPHVASLFFAEAMVYSVIGGLGGYLIAQ
                     GSMKLLGLAATYGLVRVPEMNYSSTNAIVTILIVMATVLVSAIYPAIKASRSANPGLL
                     RSWKLPPPKGDTFDLVFPFTVSGYDLTGVVSFLREHFESFSDTGLGSFMARDAHMTVA
                     EDGGLGIFARLALAPFDLGVTETLELRSKPSEIEGIDEINIRLTRLSGQPKDWERLNK
                     VLLDDLRTQFLLWRSLPQETMEIYRHRTLSEMKKHGA"
     gene            complement(6401..7159)
                     /locus_tag="PKK36_07795"
     CDS             complement(6401..7159)
                     /locus_tag="PKK36_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94495.1"
                     /translation="MKEEQTVQTSAGERRIIIRTIGMKKFYNRKGQITQALRGVDIAI
                     FDAEFITVMGPSGSGKSTFFNLIGALDSPTHGRVFIDEVDVAQLTAEELAFLRCRKIG
                     YIFQSYNLIPYMTALENVTTPMAFGGVQTDEARKRGMDLLELVGLKERWFHRPIEMSG
                     GQQQRVAIARALANNPTVLLCDEPTANLDHHTGDEILAILQRLNKERGVTIICATHDH
                     RMMNVCDRLLWIRDGAIERIARRDEVKVELGGID"
     gene            complement(7156..8550)
                     /locus_tag="PKK36_07800"
     CDS             complement(7156..8550)
                     /locus_tag="PKK36_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94496.1"
                     /translation="MMNRIDVQEQPVLSFRRIVRIAFSGVRYRLFRATITVAVIAIAM
                     AFLMNILGESLIKKSVAEAARSRIEELHRTDTWIARLSVPRTPEEIIFDQAGRAPVAK
                     TCLDFFSRLDYGRRRILSGSAESVQIFDLLQDAEKQESFFAALANMKAIRFPVGREEF
                     VSFLASWPELKQQIRGVREKEQAAINRIEQARGERSILEALTRADGEFGQAIRDAGFE
                     LDDREAETLAAQARDIMDSQLIEDTINHTELRQAVAARKDLLPGDITVNIIWKMLRDA
                     DSSEWYLEQMAAAGLEVSGLDAGRVRHLAERRARARLLVKAELRTADTGGGLMGIGQR
                     MTWLALVSMLVCVVGIANAMLMSVTERFREIATLKCLGALNTFIMTVFLIESSILGLA
                     GGIAGTLIGLVICVVRMFFVFQSLLAHAFPWGSLGLGALISITAGILLAAVAAVYPSL
                     RAARLAPMEAMRIE"
     gene            complement(8558..>8864)
                     /locus_tag="PKK36_07805"
     CDS             complement(8558..>8864)
                     /locus_tag="PKK36_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94497.1"
                     /translation="LVVLFTAGRHRFAWWPVHPLLFLVLGTWQSCLLAFSFLLGWGVK
                     KAVTKYGGANIYQQLKPLMFGLIAGEMLGGVLPMIVSVIYYIATDGQIPKAYQIMPG"
     assembly_gap    8865..8874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8875..10821)
                     /locus_tag="PKK36_07810"
     CDS             complement(<8875..10821)
                     /locus_tag="PKK36_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94498.1"
                     /translation="MNYRQRIWKSIFIEVQPIVSLRAVILGLLSAAFVCSYTYFNDHI
                     MRQTMFVGNSMPISVYGLLVIFLLFVFPLLRRISRRLALRRAELAVILAMTLAVCAIP
                     GSNLLRIFSPTLVLPHRFENTEPGWQKQEVMKLVPDGMLVDVKGREELVLNGFARGLR
                     EGNQAIAVREVPWRAWAKPVFFWVPVILSLWIAMAGLSLVVHRQWVDYEHLPYPIVTF
                     TKSLLPNDDGSPNPIFTNRLFWIGLLTAFLIHSYNYLVVWFPDYLFGKIPLGVDFSSL
                     TELFPTFEKGGGENFLSGYSTLFFTVIAVAYFLPKDVSLSMGIGPFLWIIIAGTLATY
                     GVGTSGWQGAWPFGIQRDAMFIMGAYLAMLLVLIYTGRHYFSNVFKRAAGFKTADHIE
                     PSAVWGARVFIVAMFFFVVYSAITAKLDWPLALLLGLGLAAVYLVMGRMIAETGLFFI
                     VVTGTPATIIWAVFGAKALGPQMMLIMFMFCTMLFYDTREALMPYIVNAIKLTDDCRV
                     NMGRTALVIVAALMLGLAVGIPVTLYFQYDLGIDISAWQNNQPKVPFNEVIGAMQRLD
                     AQGQLETAGLRTGLSRIADITPHWQGLFTMGMAIVLVLVFSFLRLRFARWPLHPVMFL
                     IWSTYAGNCFAQSFLIGWLIKSAVT"
BASE COUNT         2200 a         3332 c         3312 g         2016 t
ORIGIN      
        1 atgatgcact ggttttccgc ctcgagctcc cacatcattt cggcaaacgg atagacggcc
       61 gaaggaactt cattcagccc ccagatgaaa ttcatgaaga agatggagct gacgagaata
      121 atcggaaaca gcacaatctg tgtcttccag atgctgggga agcgggtgcc ggtcagttcg
      181 cactgcttgt agaaaacggt catctggccg tagttggcca tgggaatggg gaggaaccag
      241 caggctgccc cgtgatagcc gctcagaatc agtgccgcct cccggatata tggaacctcc
      301 accacctgtc caaccatgcc ctcaagtcgc gcggtcacat agcttatgat cggcgtgtac
      361 acaaatccca gcagcagcag tacgatcatg atgcccctgt gccagtcaat cagccagccg
      421 gacaccagga tataggtgac ggtgatgaag aaatacacaa ggatcacaag ccagacccgg
      481 atgtcgcccc ggccttcggg gacggaagac cgcgggccct cctgcgtctc tgcctgcacc
      541 ttccggttcc ggaagctgcg gaaaacctgc cagaagcccg cgatcgcgat cgccatcgca
      601 atgccgatct ggaagctgaa atgaaaatca atattgttct tgtacagcgt gttgattgtg
      661 ctgtctcccg gcgtccagtt gtgaatgatg ccgaaatgat agagcatcgg attgagaata
      721 aaggtgacaa tcagcccgat gaatgaaccc accatggcaa aaaacggcag caccatgccg
      781 aagatgatgt tgccgaaatc ccacgacatg cccgtggcca cggccggaag atatcgtccg
      841 gtcttggccg tgaagtcgga gaacggaatc tgaaatatct ggatcggctc tccggtcagc
      901 gcgcccgtca gcgtgggcag cagcaggtaa accgtcccga aaagaagtcc cagcgcgccg
      961 ccgatggaaa acacgcgcca gcgccattga gacgcgcggg attcaaggtt cttctcgtct
     1021 gcatcctcgg ccagcgcgat gatgccctgc gcgccaatgg gcgccatggg aaagggcagg
     1081 cgctccacgt cgctggcgag ccggaaaagc ccgtacccga gcaccatgtt tgccagctgg
     1141 ccgaaaaaag tgccgaatat gatcaggccg atcaccggga gccagtcggc gtgaatgaat
     1201 gttctcgcca cataggactc cgagtccgcg ggcggggcat accagatggg aagctggtct
     1261 gcgatgcccg ccgcgatggc cgcatcgctc cgggcgaaga actggttcca gatgaggccg
     1321 gagaagggca tccccatggc cgcgcccgcc atgaagaaga gcacgaatat ctccgccttc
     1381 ttcatggtct tgtgcgcgcg tttggccacc tcgatgaaaa gaatcacagt cacccactgc
     1441 gaggccgcgc caatgttgcc cacgccggcc agcaggccca tatagatggc ccccggaacc
     1501 atcagcagcg cgataaagat cgcgccgatc agggcggtcc agttgaagcc ctcctcgaac
     1561 gatgagggga cttccatcac cgaccggaat tcctccagtt ctttgtctct tctatgcgcc
     1621 atgctttttc atctctgaaa gggttcggtg tctgtaaatt tccatggtct cctgcggaag
     1681 cgagcgccac agcaggaact gcgtccgcag atcatccagc agcaccttgt tcaggcgctc
     1741 ccagtccttc ggctggccgg acagccgcgt caggcggatg ttgatttcgt caatgccctc
     1801 aatctcgctg ggtttgctgc gcagttcgag ggtttccgtc acgccgagat caaagggggc
     1861 cagggccagc cgggcgaata ttccgagccc tccgtcttcc gcaaccgtca tgtgcgcatc
     1921 cctcgccatg aaggagccga ggccggtgtc gctgaaggat tcaaaatgct cgcgcagaaa
     1981 actgacgacg ccggtcaggt catagcctga caccgtgaag gggaaaacga gatcaaaggt
     2041 gtcgcccttc gggggcggaa gcttccacga ccgcagcagg cccggattgg cgcttcgcga
     2101 ggccttgatg gccggataga tggcggagac cagcaccgtc gccatgacga tcaggatggt
     2161 gacaatggca ttggtggagg aatagttcat ctccggcacg cgcaccagcc cgtacgtggc
     2221 ggcaaggccc agcagcttca tggatccctg cgcgatcaga taacctccca gtcccccgat
     2281 cacagagtag accatcgctt cggcaaagaa caggctggcc acatgcggcg gggcaagacc
     2341 cagcgcgctg aaggtgaaaa tttccttctc ccggtcggcc acggatccga gcattgtccc
     2401 gaagatcacc aggccgccca gcagcaccgg gaagaacagg tcgccgactc cgctggcggc
     2461 catcacggta cccagaatat gccggtacac gccgtcggtt cgtgtggcca gaacaggggt
     2521 cttgtcctgc atgcgggcgg cctcctcggc gatgtccgag gcctgtccgg catcctccgt
     2581 gtaaatcgcg atcaggtgcg gcttgccgcc cgcgcgccgg gtgttttcgg ccgaggtgat
     2641 gataatggag tccaccggga gactggtcca ggtatcctgc tcctgggtca tgtcgtccga
     2701 ctgaacggtc tggtccttct gtgccgcctt catctgcgcg aaatccacgg gaaggatact
     2761 gctgccgtcc atgtcgcgcg ccgttgagag agcggatgcg ctgatcagcg gtccgacctt
     2821 caggcgcagg cccttcagga gaacctcgtc gcccggtttc acattcagca gttcggccac
     2881 ggcttcggtc atgaaggcat ggctgtccag atcatccccg tctgtgatct gcagcagcgc
     2941 cttcaggtcg tcgcggcggc ggatctcctg cggcgacagg ccgagcatgc ccttgagggt
     3001 gaccggattt gatccgtcgg cgagggccac cacgaaatcc ggatcgcgtg caaattccgg
     3061 gcatatccag aaccgcgtgc agacctccgc gtctttctcc caccggctcc ggatgacatc
     3121 cacaaactcg agattgtagg acccccaggt cggattgcgc agaaccgctc cggtataggc
     3181 cggggatggc gcggaaaaga gcttcatgat gcccttgcgc gtgtcgaacg atgcgaaaca
     3241 gaggatggtg aaggtcagaa gaataatggt gatggccgtg agcgcggtgc ggagcggccg
     3301 ccggcgcatg gtggaaatgc ccatggccat ggcggccatg accgtgttga atcgggatat
     3361 gtccgcggcg tgaatgctgg cggtcattcc ctgcagggcc ttcagctccg cctcgaattt
     3421 gcgcatgatg atgaaaatca ccagaacgga gagaagcacg acggcgaatc cgaggaaaat
     3481 gataaccggc gttttggcga tggcaaaggc ggggtgcgag agatagagaa tgatgaaggt
     3541 cagcgtgaag aatcccgtga accagccaac ctgcttgtag atcatgtttg aaccgatcag
     3601 aagccgctcc agtgcgaagg caaacggcac gcacagggcc agaagcacca gcaccgcctt
     3661 gaccagatcg tccattgacg tgcggatcat ttcataaacc ggggttgcgc tcatgaaggc
     3721 ggatgccgcc agcgcctcct tcagcaccgg agagtccgag cgtgccgctt cgtccaggct
     3781 gtcgcggatg cggccgtgca gttcttccag cgaactgttc aggatgtccc ggcttcgcag
     3841 aatggccagg cgcccctcgt tcaggcgcca gagatcccat gccgactgtt cactgttttt
     3901 cagcgacagc cagtcggtgc ggccggccgg ataacccgcg ccgtacattt ccgcgccgcc
     3961 ttcaaccggg cgatcgttga tcagcgccac aatggaattg aggccgaagg cctttatgcc
     4021 tttcagcttt tcctccgcaa accagtaaac gactccgtcc gcggtctcgg catatgcttt
     4081 gtctgaatcg tccccggaga tcgcggcatt cgcccgcgca ttcatgacag acgcgttctg
     4141 cggccagaat gacgggggga gcatcagcag cccgctgttc acgttcagca tgttcaggcg
     4201 tgtcagcacg gaccggtagg atttgttttc ggacgtcatg accacccgcc cgctcccgtc
     4261 aaagctcgcg gaaaaaccga agatgctgta ttcgtcctcc ggcagcgaac ccgcgctgta
     4321 ggccccgttt tcgtcggtca tggtcacgat gaacgggtca aaggcatagg gcttgttgtg
     4381 cggccagaag cagttgttcc acggatcaac cagcgatatc tgcaccaccg cgccgggcac
     4441 cggccggttg ggaatggagc ttccgcgctg gcgccccatg accagcgcgc cggtcgcgcg
     4501 cccgttttca aagcccgctg tgatgtactg cttgtccgcc gcaatggcgc gccgcagcga
     4561 aaggccctcc tggtctgcga cggcttcaac ggctccgagc tgtgtttcaa gactggccac
     4621 agccgccagc agcagcgcga tctctcgtgt ggtctggcgg atgatgtccg tgttcaggaa
     4681 ttccgctgta tcgtccggcg ttccctcgcg cggaagcgtt tcgtgcatcg tgcccaggac
     4741 gatgttgtag atgccgtgcc gtccggcgat ttccccgccg tggatcagca tcggcgcgtt
     4801 ccagagaagc cgggtctggg tcaggcgtcc gtccaccgag gccggctcaa aatgcgcggc
     4861 ccgggtcttg cccttttcca gcgactccca cgcccgcagg aagctgccct gtatccggcc
     4921 atacagtccc ggatcatcct gcgcggaatg aatgtccgca tcgccgccga tcaccacacc
     4981 ccagcgctcg gtggtgtcgc ccagcatcag ggaaatgtga agcgcgatcc atcggtcggc
     5041 cagcaggtcg cgcactttca gggaggaatc cagcatggcc ttcgcggccc gcagttcact
     5101 ggcgcgggcc tccacgctgg cgtgaaccgt tttcaaaacc tccctgaaca ggggctggga
     5161 attttctgac acggagccgc gtccgagttc gcggcgcaga tcgttccacc ggttctgggc
     5221 ggaggccagt tcgtcaatcc tggcggagaa gtcggcggat tcatcctcct tctccttcgc
     5281 gcggagttcc atcagctgtc cccggatctc ctgcgcgtaa tcggttgccg cgctccgcag
     5341 gcgcttcagc agttccttgc cgctggggga tatcctgtcg gccagcgggt catccttttt
     5401 catctgttcg cgcacctgcc ggatgaagga agattcggtt ttccacgcct tcatgcgcgc
     5461 gtcgagatcc gcctgcttga ccttgtcctc aagcgcctcg taaaaagccc ttgctccggc
     5521 gtgggaacgc gcctggccgt ccgcaaacaa gaacaccgcg ccgcgccgcg gcctctgatc
     5581 ccggagcatt tccgcaagct gcagaagcgc cgcgcagttg gcggccggcc gcgcgcccgg
     5641 ggtgcgttcg ggaatctccc cgaagctgtc cagattcgcg ctgatgatga tgacctcttc
     5701 cttgccgagg tcaaaaacag gatccgtgcc ctttaaatat ccaatgacgt ttcgccccgt
     5761 ggtcttttcc cagacaatct cgctgtgaag agaaacccgg gcgccttctg tcagattgga
     5821 cgcgggcccg tcataataga atctgggcag gttggcgttg gcgcgcacgt gatgatggtg
     5881 tcgtgattcg cagggctcct gacgggtgaa gataaccgct tttgcgccca gccggaacgc
     5941 cttgagccac tgatcgtcgg tgttgtagtc catcaccgcg atcttgccct gcgggtcggc
     6001 ccgctcgaaa tctccggcgt gtcccgcgcc cagccagacc agttcgccgg tgatgccctc
     6061 cgggctgctg accggcggga taaccccatc cgggcgcgcc ggaaccagtg gaattttctg
     6121 tccgccgtca aataccagtt cgcaccgttt ggtgacggtc tgggcggcgg gaaattcctg
     6181 aacgatgacc tcgtccacgc cgatctgctc caggcgggct ttgacgtgat ccgcggcggc
     6241 ccggtattcc atcgtgcccg taagccggtg cggtccgcgg gtcagcgcct cgaggtcggc
     6301 ggggaacgag ccgctgtcgg cgacggccgc aagagccagc gccggcatca gtcccgccat
     6361 cattttcagt ttcttcagca cgttttccat tctccagaaa tcagtcaatg ccgcccagtt
     6421 ccaccttcac ctcgtcgcgc cgcgcgatgc gctcaatggc gccgtcgcgg atccacagca
     6481 ggcggtcgca gacattcatc atgcggtgat cgtgcgtcgc gcagatgatg gtgacgccgc
     6541 gctccttgtt cagccgctgc aggatcgcca ggatttcgtc gccggtgtga tggtcaagat
     6601 tggcggtcgg ttcatcgcaa agcagcacgg tcggattgtt cgccagcgcg cgcgcgatgg
     6661 ccacccgctg ctgctggccg ccggacattt caatgggccg gtgaaaccat cgctccttga
     6721 ggccgaccag ttccagcagg tccattccgc gcttgcgggc ctcgtcggtc tgcaccccgc
     6781 cgaaggccat cggggtggtg acattctcca gcgcggtcat atagggaatc aggttgtagc
     6841 tctggaagat gtagccgatc ttccggcagc gcagaaacgc cagctcctcc gcggtgagct
     6901 gcgccacatc cacctcgtca atgaacaccc gcccgtgcgt cgggctgtcg agcgccccga
     6961 tcaggttgaa gaaggtggat ttcccggagc ctgacgggcc catcaccgtg atgaactccg
     7021 catcaaagat ggcgatgtcc acgccgcgca gcgcctgggt gatctgcccc ttgcggttgt
     7081 agaacttctt catgccgatg gtccggatga tgattcggcg ctcgccggcg gaagtctgga
     7141 ctgtctgttc ctctttcatt cgatcctcat ggcctccatg ggcgcgagcc gcgcggcgcg
     7201 gagcgacgga tacaccgccg cgaccgccgc cagcagaatg cccgccgtga tggagatcag
     7261 cgcgcccagc cccagcgatc cccacgggaa ggcatgggcc aggagcgact ggaaaacaaa
     7321 aaacattctg accacgcaaa tgaccaggcc gatcagcgtg ccggcaatgc ccccggcgag
     7381 cccgaggatg gacgattcga tcaggaacac ggtcatgatg aaggtgttga gggcccccag
     7441 gcatttgagc gtggcgattt cgcggaaccg ctccgtcacg gacatcagca tggcgttggc
     7501 tatgcccacc acgcagacca gcatggaaac cagcgccagc cacgtcatgc gctggccgat
     7561 gcccatcagc ccgccgcctg tgtcggcggt ccgcagttcg gctttgacca gcagccgggc
     7621 ccgggcgcgc cgctcggcga gatggcgcac ccggccggca tcgagtcccg aaacctccag
     7681 gccggcggcg gccatttgtt ccagatacca ctcactgctg tccgcgtcgc gcagcatttt
     7741 ccagatgatg ttgaccgtga tgtcgcccgg gagcaggtcc ttgcgcgcgg ccacggcctg
     7801 gcggagctcc gtatggttga tcgtgtcctc gatgagctgg ctgtccatga tgtcgcgcgc
     7861 ctgagcggcc agcgtttcgg cttcccggtc gtcgagttcg aagccggcgt ctctgatggc
     7921 ctgcccgaac tcgccgtcgg cccgcgtcag cgcctcaaga atggagcgct cgcctcgggc
     7981 ctgttcgatg cggttaatgg cggcctgttc tttttcccgg accccgcgga tctgctgttt
     8041 cagttccggc catgatgcga ggaacgaaac aaactcctcc cggccgacgg gaaagcggat
     8101 ggccttcatg ttggccagcg ccgcgaaaaa ggattcctgc ttttcagcat cctgaagcag
     8161 atcgaatatc tgcacggatt cagcgctgcc ggacagtatt ctgcggcggc cgtaatcgag
     8221 gcggctgaag aaatccagac aggttttcgc gaccggcgcg cggcccgcct gatcaaatat
     8281 gatttcctcg ggtgtgcgcg gaaccgaaag ccgcgcgatc caggtgtcgg tgcggtgcag
     8341 ttcctcgatc cgggagcggg ccgcttcggc cacggatttc ttgataaggc tctcgccgag
     8401 gatgttcatc aggaaggcca tggcgatggc aatcaccgcc acggtgatcg tggcgcggaa
     8461 caggcggtag cggacccccg agaaagcgat ccgcacaatc cgccggaagc tcagcaccgg
     8521 ctgctcctgt acatcaattc tgttcatcat caaaacatca tcccggcatg atctggtatg
     8581 ccttggggat ctgtccgtcc gttgcaatat agtaaatcac gctgacgatc atcggaagca
     8641 ccccgcccag catttcgcca gcgatcagtc cgaacatcag cggcttgagc tgctggtaga
     8701 tgttcgcccc gccgtatttg gtcacggcct tcttgacgcc ccacccgagg aggaaggaga
     8761 aggcgagcag acaactctgc caagtcccca gcacgaggaa caggagcggg tggaccggcc
     8821 accacgcgaa ccggtgccgg ccggcggtga acaacaccac gaggnnnnnn nnnnggtcac
     8881 ggccgacttg atcagccatc cgatcaggaa cgactgggcg aagcagttgc ccgcataggt
     8941 ggaccagatc aggaacatca ccggatgcag gggccagcgc gcgaagcgca ggcggaggaa
     9001 tgaaaataca agaaccagga cgatggccat gcccatcgtg aacaggccct gccagtgcgg
     9061 agtgatgtcg gcaatgcggc ttaagcccgt gcgcaggccg gcggtttcaa gctgcccctg
     9121 agcgtcgagc cgctgcatcg cgccgatgac ctcgttgaac ggaaccttgg gctggttgtt
     9181 ctgccacgcg gaaatgtcta taccgaggtc gtactggaaa tagagcgtca ccggaatgcc
     9241 cacggccagc ccgagcatca gggcggcgac aatgaccagg gcggtcctgc ccatattcac
     9301 ccggcagtcg tccgtgagct tgatggcgtt gacgatgtag ggcatcagcg cctcgcgtgt
     9361 gtcgtagaat agcatcgtgc agaacatgaa catgatcagc atcatctgcg ggccgagcgc
     9421 ctttgcgccg aacaccgccc agatgatcgt ggccggggtg ccggtgacca cgatgaagaa
     9481 aagcccggtt tcggcgatca tgcgtcccat caccagatag acggcagcca gcccgagccc
     9541 cagcagaagc gcgagcggcc agtcgagctt tgcggtgatc gcggaataga ccacaaagaa
     9601 aaacatcgcg acaatgaaaa cccgcgcgcc ccagacggcc gaaggttcaa tgtggtcggc
     9661 ggtcttgaaa cccgcggcgc gcttgaacac gttggagaaa tagtgccgcc cggtgtagat
     9721 cagcaccagg agcatggcga gatacgcgcc catgatgaac atggcgtcgc gctggatgcc
     9781 gaagggccac gcgccctgcc agccggaagt gccgaccccg taggtggcca gcgtgccggc
     9841 aatgatgatc cacagaaacg ggccgatgcc catcgagagc gacacgtcct tggggagaaa
     9901 ataggcgacc gcgatgactg tgaaaaacag tgtgctgtag ccggaaagaa agttttcgcc
     9961 gccgcctttc tcgaatgtgg ggaagagctc ggtcagcgag ctgaaatcca cgcccagcgg
    10021 aattttgccg aagaggtagt ccggaaacca gaccacgaga tagttatagg aatggatcag
    10081 gaacgcggtc agcaggccga tccagaaaag gcggttggtg aagatcgggt tgggagagcc
    10141 gtcgtcgttg ggaagcagcg atttggtgaa cgtgacgatg ggatagggca ggtgctcata
    10201 atccacccac tggcggtgca ccaccaggga aaggcccgcc atggcgatcc acagggaaag
    10261 aatgaccggc acccagaaga aaacgggttt ggcccacgcc ctccacggaa cctcgcggac
    10321 agcaatcgcc tgattgcctt cgcgcagtcc gcgcgcgaag ccgttcagca caagctcctc
    10381 gcggcccttc acatccacca gcatgccgtc gggaaccagt ttcatgactt cctgcttctg
    10441 ccagccgggt tcggtgttct cgaagcggtg cggaagcacg agggtcgggc tgaaaatgcg
    10501 cagcaggttc gatccgggaa tggcgcagac cgccagggtc atcgcgagga tcaccgccag
    10561 ctcggcgcgg cgcagcgcca gtcttctgct gatcctgcgg agcaggggga aaacaaacag
    10621 caggaaaatc accagcaggc cgtatacgga aatgggcatg ctgttgccga caaacatcgt
    10681 ctggcgcatg atgtggtcat tgaaatacgt gtagctgcag acaaaagccg cactcagcaa
    10741 acccagaatg accgcgcgca atgacacaat cggctgaacc tcaataaaga tgcttttcca
    10801 aatccgttgc cggtaattca tgaatttgta ccagactttt tcgggaagtg aaacgctaaa
    10861 cctgattttt
//
LOCUS       DAOQWR010000087        10843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6517_length_10843_cov_4.885521, whole genome shotgun sequence.
ACCESSION   DAOQWR010000087 DAOQWR010000000
VERSION     DAOQWR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6517_length_10843_cov_4.885521"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3393
                     /gene="rpoC"
                     /locus_tag="PKK36_07815"
     CDS             <1..3393
                     /gene="rpoC"
                     /locus_tag="PKK36_07815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR94499.1"
                     /translation="LEGGRFATSDLNDLYRRVINRNNRLRNLLQLKTPDVIIRNEKRM
                     LQEAVDALFDNGRHGRAVTGAGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIV
                     VGPDLDMRQCGLPKKMALVLFEPFIIRRLKEKGIVHTVRSAKKMIERADDVVWDILEE
                     VTRGHTVMLNRAPTLHRLSIQAFEPVLIEGEAIRIHPLVCTAYNADFDGDQMAVHVPL
                     SVEAQLESRLLMLAPNNIFSPSSGKPITTPSQDIALGCYYMTMESQQDKIRQLKKKAG
                     GDEVEHLPILASIDEVHTAFDEGALKLHSRIRFRNPDYQRETLYGSAGKSVITTTVGR
                     VFFNEIWPECMGFINQKVTKKNLENLIRQCYLRAGHDETVQVLDRLKNAGFEFATLGG
                     ISIGLTDMIIPPEKQKIIADSREQIAAVDAQYRKGVITDGERYNKIIDIWTHSTEEIA
                     HVMFSVMEYNENKETPNPIYMMVDSGARGSRQQIRQLAGMRGLMAKPSGEIIERPILS
                     NFREGLSVLEYFISTHGARKGLADTALKTAESGYLTRKMVDVAQDIIVVEQDCNTLNG
                     IQMKAIYEGDEELVPLANRITGRTSVDDVYDPRTREIIVAAGQEIDARAAEAIMTADI
                     RMVKIRSVLTCESSRGCCAKCYGRNMATGEPISLGSAVGIIAAQSIGEPGTQLTMRTF
                     HIGGTASSVFKQPQIIAKHDGYVRYQDMRTVQVADGSFVVLNKNSTLGVYDDEGRELD
                     HYTIVLGAVISVPDGGKVKKGTSFVRWDPYNVPILSEQDGQIKFRDFIEDVTFRRETD
                     EATGLEGTVVMEHKEDLHPQILLVRPGTKEILNYYSIPSGAVVVVKEGEKVVAGAMLA
                     KTPRQVSRTKDITGGLPRVAELFEARRPKDAAEISKIEGVVEFAGSVRGKRRVLVRDP
                     ITQLEEEHLIPMGKHVVVFQGDRVQKGQQLTEGPVVPQEVLEICGPQELQEYLVNEVQ
                     EVYRLQGVEISDKHVEVIVRQMLRKVRITDPGDTDFLWGEQVERYYFHEKNQQAMAEG
                     KRPAEASPVLLGITKASLETESFISAASFQDTTRVLTDAATLGRVDQLMGFKENVIMG
                     HLIPAGTGFHLYRNIRLVPLAPPISAEELLGDTLPDYSAANE"
     gene            3475..3849
                     /gene="rpsL"
                     /locus_tag="PKK36_07820"
     CDS             3475..3849
                     /gene="rpsL"
                     /locus_tag="PKK36_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006795815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR94500.1"
                     /translation="MPTINQLVRKGRSRVTQKSKSRALAQCPQRRGVCLLVKTQTPKK
                     PNSALRKIARVRLTNGEEVTAYIPGEGHNLQEHSMVLVRGGRVKDLPGVRYHIVRGTL
                     DCLGVDGRNRGRSKYGCKKVKK"
     gene            3862..4332
                     /gene="rpsG"
                     /locus_tag="PKK36_07825"
     CDS             3862..4332
                     /gene="rpsG"
                     /locus_tag="PKK36_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR94501.1"
                     /translation="MARRRRPSRRETAPDPRYNSKLLGRFITNIMKWGKKSKAEALVY
                     GALDLIAEQTQEEPLKVFEQAVENIKPRLEVKSRRVGGATYQVPVEVSWDRQVTLAFR
                     WLIMYARARKGMGMRKALAIELVDAYKNQGSCIKKRDDTHKMAQANKAFAHYRW"
     gene            4360..6498
                     /gene="fusA"
                     /locus_tag="PKK36_07830"
     CDS             4360..6498
                     /gene="fusA"
                     /locus_tag="PKK36_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR94502.1"
                     /translation="MVTVIEPDTKQTGKDAASRTEADGRRCALDRIRNIGIMAHIDAG
                     KTTVTERILYYSGRLYKMGEVHEGTATMDFMPQEQERGITISSAATSCRWLDHQINII
                     DTPGHVDFTVEVERSLRVLDGAVAVFCGVAGVQPQSETVWRQAQKYKVPRLAFVNKMD
                     RKGAVLDSVVTQMKERLAAPAEALQMPIGSEENFEGVVDLVHMKALRFATEDLGARAV
                     ESEIPDGLYAEALARRARLIEAVADRDEELLHLYLEQPEVPEEPLMAAIRRQTVFGRF
                     VPVLCGSALRNKAVQPLLDAVVHYLPSPLDVPAVTGHDLKDEAKTVVRKSSDFEPLSA
                     LAFKISADAYVGKLTFVRVYSGQLKKGQNVFNPRTRKRERLGRILQLHANHREEVDVL
                     YAGEIGGIAGLKNITTGDTLCAENQPVILERIEFPEPVMSMAIEPKTSADREGLQQAL
                     AVMAAEDPTFRTVIDPETGQTIIHGMGELHLEIIKDRMFREHRVQARAGRPMVAYHET
                     VRATARAEHVFQREIAGRGQFAQITLEVAPRSRGTGNKIEFQVSADRIPTEFREAVEE
                     GVKDGLVTGVLGNYPLTDLLVKVVDGKFHPVDSTELAFRMAAVMALRDASSSALPAIL
                     EPIMAVEIITPEEYMGDVLGDVNSRRGRIKEMALKEAVQIIRAEIPLAELFGYSTALR
                     SLTKGRASYSMEPEMFEVVPETIQESILNR"
     gene            6506..6814
                     /gene="rpsJ"
                     /locus_tag="PKK36_07835"
     CDS             6506..6814
                     /gene="rpsJ"
                     /locus_tag="PKK36_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNR94503.1"
                     /translation="MNGQRIRIRLKAYDHRVLDQSAADIVETAKRTGARVAGPIPLQT
                     KIERYTVNRSPHGDKKSMDQFEIRTHKRLLDIIGPTAKTVDELKKLNLPAGVDITIKI
                     "
     gene            6823..7467
                     /gene="rplC"
                     /locus_tag="PKK36_07840"
     CDS             6823..7467
                     /gene="rplC"
                     /locus_tag="PKK36_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNR94504.1"
                     /translation="MNALIGRKLGMTQVFDQTGAQVPVTVIQAGPCVVVQRKTLEKDG
                     YEAVQIGYEEQKESRLTRPVAGHFRKGGITPRRVLREVRLEDGGSDTKVGDVFSAEIF
                     EGAGFVDVIATTKGRGFQGVVRRHGMIGGRASHGSGNHRKPGSIGMREHPGRIFKNKR
                     MPGHMGSTQVTTQNLELVQVRKDDNLLLVRGSVPGPKGGIILVRKSIKKASRAS"
     gene            7464..8108
                     /gene="rplD"
                     /locus_tag="PKK36_07845"
     CDS             7464..8108
                     /gene="rplD"
                     /locus_tag="PKK36_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNR94505.1"
                     /translation="MSKIPLRNIKGEELGAYTLADSLMVLDKGLQAMHDSVVAHMAAR
                     RAGTASTLTKAEVSGSNKKPWRQKGLGRARSGYRQSPVWRGGGVAFGPKPRSYRKKMT
                     KKAARLGFCRAFSEKVAANEVIVLDELNLAAPKTREFAAILKKLGADRGALVLVDRIE
                     ENIRRASGNLPRVEVCEARNVNTYQILRYPVVVVTKSGMADMEKRLTSDAGSDA"
     gene            8105..8389
                     /gene="rplW"
                     /locus_tag="PKK36_07850"
     CDS             8105..8389
                     /gene="rplW"
                     /locus_tag="PKK36_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNR94506.1"
                     /translation="MKDSYSIVKKLMLTEKGTRLSESENKYLFRVDRSANKVDIKRAV
                     EELFKVGVVAVNTMNRQGKKKRERTPNFGRTANWKCAVVTLKPGDKIDLT"
     gene            8406..9233
                     /gene="rplB"
                     /locus_tag="PKK36_07855"
     CDS             8406..9233
                     /gene="rplB"
                     /locus_tag="PKK36_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNR94507.1"
                     /translation="MALKKYRPTTPSRRFTVLSDFAEITKSKPERSLIEPQKKQAGRN
                     RAGRITVRHRGGGHKRHYRIIDFKREKHGIEAKVSAIEYDPNRSANIALLTYRDGEKR
                     YILAPLGLKVGDKLMSGPDAEPSVGNSLPLARIPLGMAVHNVEMQAGRGGKMVRSAGG
                     SATLMAREGGMATLKLPSGEIRLVKDDCSATIGQMGNVEHENISMGKAGRKRHMGIRP
                     TVRGVAMNPVDHPMGGGEGRTSGGGHPRSPWGQLAKGKRTRVRSKASSKMIVQRRKK"
     gene            9237..9521
                     /gene="rpsS"
                     /locus_tag="PKK36_07860"
     CDS             9237..9521
                     /gene="rpsS"
                     /locus_tag="PKK36_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNR94508.1"
                     /translation="MARSIKKGPFVDAKLIKKVEAMNQGGIKRPIKTWSRRSMIVPEF
                     IGHTFAVHAGKAFVSVFVTENMIGHRLGEFAPTRTFRRHGLATDKTVSLK"
     gene            9635..9982
                     /gene="rplV"
                     /locus_tag="PKK36_07865"
     CDS             9635..9982
                     /gene="rplV"
                     /locus_tag="PKK36_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNR94509.1"
                     /translation="MEVIAVTKFVRLSPSKARDLTRAIQGRPVAEALSVTEVSRRKAA
                     REIQKTLKSAIANAENNAKLSVDKLYIKEAVIEQGPSMKRFWPRARGMVSPIRKKLSH
                     VRIVLSDEKKARA"
     gene            10010..10672
                     /gene="rpsC"
                     /locus_tag="PKK36_07870"
     CDS             10010..10672
                     /gene="rpsC"
                     /locus_tag="PKK36_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNR94510.1"
                     /translation="MGQKVNPIGLRVSVNKDWRSRWFADNKQYGDLLHEDIKIRETVA
                     ERLKDAAVSEVRIERYANRARITVYTARPGIVAGRRGEDIERLRADLAKLTGKEIYIE
                     IQEVKDPDKNAQLVAQNIATQMERRVSFRRAMKRAIQMAMETGALGIKVECGGRLGGA
                     ELARREKYKEGKIPLHTLRANVQYGFAEAHTLAGKIGVKVWICNEPEAEVKEKKSYAP
                     YA"
     gene            10656..>10843
                     /locus_tag="PKK36_07875"
     CDS             10656..>10843
                     /locus_tag="PKK36_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L16"
                     /protein_id="HNR94511.1"
                     /translation="MPLMPNRTKYRKAQRGSRRGNAQTGNSIAFGEYGLQALGRGWIT
                     NVQIEACRVAINRSMKRKG"
BASE COUNT         2486 a         2880 c         3437 g         2040 t
ORIGIN      
        1 ctggagggcg gccggtttgc gacatcggat ttgaacgatc tctaccgccg tgtgatcaac
       61 cgcaacaacc gtttgcggaa cctgctgcag ctgaaaacac cggatgtgat catccgcaac
      121 gagaaacgca tgcttcagga ggcggtggat gcgctgtttg acaacgggcg ccacggccgg
      181 gccgtgaccg gcgccggcaa ccgtccgctg aaatccctca gcgacatgct caagggcaag
      241 caggggcgtt tccgccagaa cctgctgggc aagcgtgtgg attactccgg ccgttccgtg
      301 attgtggtcg gaccggatct ggacatgcgc cagtgcggcc tgcccaagaa gatggccctg
      361 gtgctttttg agcccttcat catccgccgt ctcaaggaaa aggggattgt gcacaccgtt
      421 cgaagcgcga agaaaatgat cgaacgcgct gacgatgtgg tgtgggatat tctggaggaa
      481 gtcacccgcg ggcatactgt tatgctgaac cgcgctccga ccctgcatcg tctgagcatt
      541 caggcttttg agccggtgct gatcgagggc gaggccatcc ggattcatcc gcttgtctgc
      601 accgcgtaca atgcggactt cgacggcgac cagatggccg tgcatgtgcc gctgtctgtg
      661 gaggcgcagc tcgaatcgcg cctgctgatg cttgcgccga acaacatctt ttcgccttcg
      721 agcgggaaac cgatcaccac accctctcag gatattgcgc tgggctgcta ttacatgacc
      781 atggagtcgc agcaggataa aatccggcag ctcaagaaaa aggcgggagg cgatgaagtc
      841 gagcatctgc cgattctcgc cagcattgac gaagtgcata ccgcctttga cgagggcgcg
      901 ttgaaactgc acagccgcat ccgcttccgc aatcccgatt atcagaggga gacgctttac
      961 ggctcggcag gaaaatcggt catcaccacg actgttggcc gggtgttctt caacgaaatc
     1021 tggcccgagt gcatggggtt catcaatcag aaggtcacca agaagaatct ggaaaacctc
     1081 atccgccagt gctatttgcg ggccggccac gatgaaaccg tgcaggttct cgaccgcctg
     1141 aaaaacgccg gttttgaatt cgccacgctg ggaggcatct ccatcgggct gacggacatg
     1201 atcattcccc ccgaaaagca gaaaataatt gccgactccc gcgagcagat tgccgctgtg
     1261 gatgcgcagt accgcaaggg tgtaatcacg gacggcgagc gctataacaa gatcatcgac
     1321 atttggacgc acagcacgga agaaatcgcg catgtgatgt tcagcgttat ggaatacaac
     1381 gagaacaagg aaacccccaa ccccatctac atgatggtgg actccggcgc ccgtgggagc
     1441 cgtcagcaga tccgtcagct ggccggcatg cgcggtctga tggccaagcc ttcgggcgaa
     1501 atcattgagc gtcctattct gtcgaacttc cgcgagggcc tcagcgtgct ggagtatttc
     1561 atcagcacgc acggcgcgcg caagggtctg gccgacaccg cactgaagac agccgaatcc
     1621 ggttacctga cgcgcaagat ggtggatgtg gctcaggaca ttattgtggt tgagcaggac
     1681 tgcaacacct tgaacggcat tcagatgaag gccatttatg agggggacga ggaactggtc
     1741 ccgctggcga accggattac gggccggact tcggtggacg atgtctatga cccgcgcacc
     1801 cgcgagatca ttgtggccgc aggccaggaa atcgatgccc gcgcggcaga ggcgattatg
     1861 accgccgaca tccgcatggt gaaaatccgc agtgtgctga cttgtgaatc ctcgcgcgga
     1921 tgctgcgcga aatgctatgg ccggaacatg gccaccggcg agccgatttc tctcggttct
     1981 gcggtgggca tcattgccgc gcaatccatt ggagagcccg gcacccagct gacgatgcgg
     2041 acgttccata tcggcggaac ggccagttcc gtgttcaagc agccgcagat tatcgcaaag
     2101 cacgacggat atgtccgcta tcaggacatg cgcacggtgc aggtggcgga tggaagtttt
     2161 gtggtgctca acaagaacag cacgcttggc gtttacgacg acgagggccg cgagctggat
     2221 cactatacaa tagtgcttgg cgcggtgatc tccgtgcccg atggcggcaa ggtgaaaaag
     2281 ggcaccagtt ttgtccgctg ggatccctac aacgtgccca ttctttccga acaggatgga
     2341 cagatcaagt tccgtgattt catcgaagac gttacgttcc gtcgtgaaac cgatgaggcc
     2401 acgggtcttg agggaaccgt ggtcatggag cacaaggagg atttgcatcc gcagatcctg
     2461 cttgtcaggc ccggcacaaa ggaaatcctg aattactaca gcattccatc tggagccgtg
     2521 gttgtggtga aggaaggcga gaaggtggtg gccggcgcca tgcttgcaaa gacgccgcgc
     2581 caggtttcac gcacgaagga catcacgggc ggtctgccgc gcgtggccga actgtttgaa
     2641 gcccgccgcc cgaaagatgc cgctgaaatt tcaaagattg aaggggtggt cgaatttgcc
     2701 gggagtgtgc gagggaagcg gcgcgttctg gttcgtgatc cgatcacgca gcttgaggaa
     2761 gagcatctga ttcccatggg caagcatgtg gtggtctttc agggcgaccg tgtgcagaag
     2821 gggcagcagc tcaccgaggg cccggtggtc ccgcaggaag tgctcgaaat ctgcggtccg
     2881 caggaactgc aggagtatct ggtgaacgag gtgcaggagg tatatcgcct gcagggagtg
     2941 gaaatcagcg acaagcacgt cgaggtgatc gtgcgccaga tgctgcgcaa ggtgcgcatc
     3001 acggatcccg gcgatacgga cttcctctgg ggtgaacagg tggagcgcta ttatttccac
     3061 gagaagaatc agcaggccat ggcggaaggg aagcgcccgg cggaggcatc tccggtgctg
     3121 cttggaatca ccaaggcttc gctcgaaacc gagagcttca tttcggcggc ctcgttccag
     3181 gacaccacgc gcgtgctgac ggatgcggct acgctgggtc gcgtggatca gctgatggga
     3241 ttcaaggaaa atgtaatcat gggacatctg attcccgcgg gcacgggttt ccatctgtac
     3301 cggaacatcc ggctggtgcc tctggctccg ccgatttcgg cggaggaact gctcggggat
     3361 acgcttccgg attacagcgc agcgaacgaa taatttcaag ttgctgttgc aaaaggacgg
     3421 aatagtttga tactgttccg ccctttgctt gtcagaagaa gggaaaaata cagaatgcca
     3481 acgataaatc aattagtgag aaaagggcgc tcacgggtga cgcagaagag caagtcccgc
     3541 gctctggcgc agtgtcccca gcgccgcggg gtttgtctgc tggtcaagac acagaccccc
     3601 aagaagccga actccgcgct gcgcaaaatt gcccgtgtcc ggctcaccaa cggtgaagaa
     3661 gtcacggcct atattcccgg cgagggacat aatcttcagg agcacagcat ggttctggtc
     3721 cgcggcggcc gcgtgaagga tctgcccggc gttcgttatc acatcgtgcg cggcacactg
     3781 gactgcctgg gagtggacgg gcgcaaccgg ggccgttcca agtacggatg caagaaggtt
     3841 aaaaaataag gaaaatttcc gatggccaga agacgcagac cctcaagaag agaaaccgct
     3901 ccggatcctc gctacaacag caagctgctg gggcgcttca tcaccaacat tatgaagtgg
     3961 ggcaaaaagt ccaaggcgga ggcgctggtc tatggcgcgc tggatttgat tgcggagcag
     4021 acgcaggaag aacccctcaa ggttttcgaa caggccgtgg agaatatcaa gccgcgcctg
     4081 gaagtgaaat cccgccgcgt cggcggtgcc acatatcagg tgccggttga agtcagctgg
     4141 gaccggcagg tgacgctggc tttccgctgg ctcatcatgt atgcgcgcgc gcgcaagggg
     4201 atgggcatgc gcaaggccct cgccatcgaa ctggtggacg cctacaagaa tcaggggtcc
     4261 tgcatcaaga agcgcgacga cacgcacaag atggcgcagg caaacaaggc ttttgcccac
     4321 taccggtggt aggttttttg aacgcgaatg gtgaactgaa tggtgaccgt gatagagccc
     4381 gatacaaagc agacaggaaa ggacgccgcc tcccggacgg aagcggacgg tcgtcgctgc
     4441 gctttggacc ggattcgcaa cattggcatc atggcgcaca ttgacgcggg gaaaaccacc
     4501 gttaccgaac gcatccttta ttattccggc cgcctctaca aaatgggtga ggttcacgag
     4561 ggcaccgcca cgatggactt catgccgcag gagcaggagc gcggcatcac catcagcagc
     4621 gcggccacaa gctgccgatg gctggatcac cagatcaata tcattgatac gccggggcat
     4681 gtggacttta ccgtggaggt ggagcgttca ctgcgcgtgc tcgacggcgc ggtggcggtt
     4741 ttctgcggtg tggccggtgt acagccccag tcggagacgg tctggcggca agcccagaag
     4801 tacaaggtgc cccgcctggc ctttgtcaac aagatggatc gcaagggtgc cgtgctggac
     4861 agcgtggtga ctcagatgaa ggagcgcctg gctgctcccg cggaagcttt gcagatgccc
     4921 atcggatcgg aagagaattt tgaaggggtc gttgatctgg ttcacatgaa ggctcttcgt
     4981 tttgcaactg aagacctcgg agccagagcc gtggagtcgg aaattccgga tggcctttat
     5041 gccgaggcgc tggcccgccg cgcgcggctc atcgaagccg tggccgatcg ggatgaagag
     5101 ctgctgcatc tttatcttga acagccggaa gtgccggaag agcctttgat ggccgccatt
     5161 cggcgccaga cagttttcgg acgctttgtc ccggtgctgt gcggttccgc gctgcggaac
     5221 aaagccgttc aaccgctgct ggatgccgtt gtgcattatc tgccgtctcc tctggatgtc
     5281 ccggctgtaa ccggtcatga tctcaaggat gaagcaaaaa cagtggtgcg aaaatcgagt
     5341 gatttcgagc cgctgagcgc gctggcattc aaaatctccg cggacgccta tgtgggcaaa
     5401 ctgacttttg tgcgcgtgta ctcgggccag ctgaaaaaag gtcagaatgt cttcaacccc
     5461 cgcacgcgca agcgcgaaag actcggccgc attcttcagc ttcatgccaa ccatcgcgaa
     5521 gaagtggatg tgctgtatgc gggagaaatc gggggcatcg ccggactcaa gaacatcacc
     5581 accggcgaca cgctttgtgc cgagaaccag cccgtgattc tggagcggat tgaatttccg
     5641 gagcccgtga tgtccatggc cattgagcct aagacgtctg ccgaccgtga gggcctgcag
     5701 caggcgctgg cggtcatggc cgctgaagac ccgaccttcc gcaccgtcat tgatccggaa
     5761 accggacaga ccattattca tggcatgggc gaacttcatc ttgaaatcat caaggatcgc
     5821 atgttccgcg agcacagggt gcaggcccgc gcgggccgtc cgatggtggc ctatcacgaa
     5881 acggtgcggg caacagcgcg cgctgaacat gtgttccagc gtgaaatcgc aggccgcggg
     5941 cagtttgccc agatcacgct ggaagttgca ccgcgttcgc gcggaacggg aaacaaaatc
     6001 gagttccagg tctccgctga ccgcatcccg acagagttcc gggaagcggt ggaagagggg
     6061 gtcaaagacg gcctggtgac gggagttttg ggcaattatc cgctgacgga tctgctggtg
     6121 aaggtcgtgg atggaaaatt tcatccggtg gattcgacag aattagcttt tcgaatggcg
     6181 gcagttatgg cattacgtga cgcttccagt tccgccctgc cggcgattct ggaaccgatc
     6241 atggcggtgg aaatcatcac gccggaagaa tacatgggtg atgtgctcgg tgatgtgaac
     6301 agccgtcgcg gacggataaa agaaatggcg cttaaggaag cggtgcagat tattcgcgca
     6361 gaaattcccc tggcggaatt gttcggatat tcgaccgcgc tgcgttccct gactaagggg
     6421 cgcgccagtt attccatgga gcctgagatg tttgaagtgg tcccggaaac aatccaggaa
     6481 agcattttga atcggtgagg ataatatgaa tgggcaacgg ataagaatac gtttgaaggc
     6541 atatgaccac agggtcttgg atcagtctgc ggcggacata gttgaaaccg ccaagcggac
     6601 cggcgcgcgc gtggcgggcc cgattcccct gcagaccaag attgagcgct atacggtcaa
     6661 tcgcagtccg cacggcgaca agaagtccat ggatcagttt gaaatccgga cccacaagcg
     6721 tctgctggac atcattggcc cgaccgccaa aacggtggac gaactcaaga agctgaacct
     6781 gccggcaggt gtggacatta ccattaaaat ctgagtgcta atatgaatgc attaattgga
     6841 agaaaactgg gaatgaccca ggtgtttgat cagacgggtg cgcaggtgcc cgtgaccgta
     6901 atacaggctg gcccgtgcgt ggtggtgcag cgcaagaccc tcgagaagga cggctacgag
     6961 gcggtgcaga tcggctacga ggaacagaag gagtcccgtc tgacccggcc ggtggccggt
     7021 catttccgga agggcggcat taccccgagg cgcgtgctgc gcgaagtccg tctggaagac
     7081 ggcgggagcg acaccaaggt gggcgatgtg ttctcggcgg agatttttga aggggccgga
     7141 tttgtggatg tgattgcaac caccaagggc cgcggatttc agggcgtagt ccgccgtcac
     7201 ggcatgatcg gcggtcgggc atcgcatggt tcgggcaatc accgcaagcc gggctccatc
     7261 ggcatgcgcg agcatcccgg ccgcattttc aagaacaagc gcatgcccgg gcacatgggc
     7321 agtacgcagg ttaccacgca gaatctcgag ctggtgcagg ttcgcaagga cgacaatctc
     7381 cttctggtgc gcggatcagt tcccggcccg aagggcggca tcattctagt gagaaaatcc
     7441 attaagaagg caagcagggc atcatgagta agatcccatt acgcaatata aaaggtgaag
     7501 agctgggtgc ctatacgctg gcggacagcc tgatggttct cgacaaaggg cttcaggcca
     7561 tgcacgactc cgtggtggct cacatggctg ctcgtcgcgc cggaacagcg tcaacgctga
     7621 ccaaagcgga ggtgtccgga agcaacaaga agccatggcg tcagaaaggc cttggccgtg
     7681 cgcgctccgg ttatcgtcag agtccggtgt ggcggggcgg aggtgtggcc ttcggtccca
     7741 agccccgatc ctaccgcaag aagatgacca agaaggctgc tcgtctcggc ttctgccggg
     7801 cgttcagcga gaaggtcgct gccaatgagg tgattgttct ggatgaactg aatctcgcgg
     7861 ctccgaagac cagggaattc gccgccattc tcaagaagct tggcgccgac aggggtgcgc
     7921 tggtgctggt tgaccgcatc gaggaaaaca tccgccgcgc atccgggaat ctcccgcgcg
     7981 ttgaggtgtg cgaggcgcgc aatgtgaaca cctaccagat cctccgttat ccggttgtgg
     8041 tggtcacgaa aagcggtatg gcggacatgg agaaaaggct gacatcagat gccgggagtg
     8101 acgcatgaag gattcctatt cgatagtcaa gaagctgatg ctcaccgaaa agggtacacg
     8161 cttgtctgaa agcgaaaaca agtacctgtt ccgggtggac cgcagcgcaa acaaagtgga
     8221 catcaagcgc gccgtggagg aactcttcaa ggttggcgtg gtggccgtca acacaatgaa
     8281 ccgccaggga aagaagaaac gtgagcggac ccccaacttc ggacgcactg cgaactggaa
     8341 gtgtgcggtc gtaacgctga aacccggcga taaaatcgat ttgacgtaag gagagcggac
     8401 aagccatggc tttgaaaaaa tacagaccca caactcccag ccggcgcttc acggtgctgt
     8461 cggactttgc ggagatcacc aagagcaagc ccgagcgcag cctgattgag ccgcagaaga
     8521 aacaggccgg tcgcaaccgt gccggccgga ttacggtgcg ccatcgcggc ggcgggcaca
     8581 agcgtcacta ccggattatt gatttcaagc gtgagaagca cgggattgag gcgaaggtgt
     8641 cggccataga gtatgatccg aaccgttcgg cgaacattgc cctgctgacc tatcgtgacg
     8701 gtgaaaagcg ctatattctt gcgccgcttg gtctgaaggt cggcgacaag ctgatgtccg
     8761 gtccggatgc ggaaccgtct gtggggaatt cgctgccgct ggccaggatc ccgctgggca
     8821 tggctgtcca caacgtcgag atgcaggcgg gccgcggcgg gaagatggtc cgaagcgcgg
     8881 gcggatcggc cacgctgatg gcgcgtgaag gcggcatggc cacactcaag ctcccgtccg
     8941 gcgagatccg gctggtgaag gatgactgca gcgccacgat cggccagatg ggcaatgtgg
     9001 agcatgagaa catctccatg ggcaaggcgg gccgcaagcg ccacatgggc attcgcccga
     9061 cggttcgcgg agtggcaatg aacccggtgg atcatccgat gggcggcggc gaaggccgca
     9121 cttccggcgg aggtcatcct cgctcgccat gggggcagct ggccaagggc aagcgcaccc
     9181 gcgtccgttc caaggcttca agtaaaatga ttgtgcagcg gaggaagaag taagctatgg
     9241 ctcgttcaat taaaaaaggt ccgttcgttg acgccaaact gatcaaaaag gtggaagcca
     9301 tgaatcaggg cggaatcaag cggccgatca aaacgtggtc tcgccgttcc atgattgtcc
     9361 ccgagtttat cgggcacacc tttgcggttc acgccggcaa ggcatttgtt tctgtttttg
     9421 tgacggaaaa catgatcggg cacaggctgg gggaattcgc cccgacgcgg acattccgcc
     9481 gccacggttt ggcgacggat aagaccgtct ccctgaagta aaggattccg gattcagaga
     9541 aatgcagatt tgtgcaagaa aggctggcaa ttcgtttttg gatgcgtaaa ttacggcttt
     9601 gcccaaggcc attcggcagt tagcgagagt acatatggaa gtgatagcag taactaaatt
     9661 tgtgcggttg tcgcccagca aggcgaggga tctcacgcgc gccattcagg gccgcccggt
     9721 ggcggaagcg ctcagcgtga ccgaggtcag caggcgcaag gcggcgcggg agattcagaa
     9781 aacactgaag tcggcgattg ccaacgcgga gaacaacgcc aagctgtcag tggacaaact
     9841 ttatattaag gaagccgtga ttgagcaggg tccttcgatg aaacgcttct ggccgcgcgc
     9901 gcgcggcatg gtgagcccca tccggaagaa gctcagtcat gtgcgtattg ttttaagtga
     9961 tgaaaaaaag gcgcgggctt agagcctgcg gcgaaaggag ggccgctttt tgggccagaa
    10021 agtaaatcca attggattaa gagtgtctgt caacaaagac tggcggtccc gctggtttgc
    10081 cgacaacaag cagtatggtg atcttcttca tgaggacatc aaaatccgcg aaaccgtcgc
    10141 agagcgcctg aaggatgccg cggtttccga agtgcgcatt gagcggtatg ccaatcgcgc
    10201 gcgcatcacg gtgtataccg cccgtcccgg cattgtggcc ggccgccgcg gcgaggatat
    10261 cgagcgtctg cgcgccgatc tcgcaaagct gacgggcaag gaaatctata tagaaatcca
    10321 ggaagtcaag gatccggaca agaacgccca gctggtggcg cagaacattg ccacgcagat
    10381 ggagcgccgc gtgtcgttcc gccgcgccat gaaaagggcc atccagatgg cgatggaaac
    10441 cggtgcgctt ggcatcaagg tggagtgcgg cggccgtctc ggcggagctg aactcgcccg
    10501 ccgggagaaa tacaaggagg ggaagattcc cctgcacacg ctccgggcca atgtccagta
    10561 cggctttgcc gaagcgcaca ctcttgccgg gaaaatcggc gtgaaggtgt ggatctgcaa
    10621 tgagccggaa gcagaagtaa aggagaagaa gtcttatgcc ccttatgcct aacagaacga
    10681 agtaccgcaa ggcacagcgc ggcagccgca ggggaaatgc ccagaccgga aacagcattg
    10741 cttttggcga atacggcctg caggcgctgg gccggggctg gatcaccaat gtccagattg
    10801 aggcctgccg tgtggccatc aaccgttcca tgaagcgcaa ggg
//
LOCUS       DAOQWR010000088        10729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6616_length_10729_cov_5.251546, whole genome shotgun sequence.
ACCESSION   DAOQWR010000088 DAOQWR010000000
VERSION     DAOQWR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6616_length_10729_cov_5.251546"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKK36_07880"
     CDS             complement(<1..191)
                     /locus_tag="PKK36_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94512.1"
                     /translation="MSGNKNIFRRMIFCALLAAALLPLAGCEDDDDYDHKPPAGMGTL
                     VLENDTVDDISVAIDGVIQP"
     gene            complement(178..609)
                     /locus_tag="PKK36_07885"
     CDS             complement(178..609)
                     /locus_tag="PKK36_07885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94513.1"
                     /translation="MISLGPCEVEVPGEAHSLALIRMVVTSLAEMAGMDENRIGEIEL
                     AVDEACSNVVDHAYSGMNPHPSFNLAIYVDDNDFIIEIIDRGKHFNFNAHVLPKFPDH
                     WDEGNVRGVGLFLIRKTVDQVTYEKLPDNRNCLKLVKHVRK"
     gene            complement(606..983)
                     /locus_tag="PKK36_07890"
     CDS             complement(606..983)
                     /locus_tag="PKK36_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR94514.1"
                     /translation="MTTCPLTVRAEDQDGFTLISVEGAVDTSNVEEFRSLIMPRCETP
                     NSRIILDCGGISYMNSRSFALLYRFHHTCESNGGIFLVCCVPLKIEHIIKLLGLNAML
                     KVLPTLEDAIDTLGTTDKGGSDS"
     gene            1117..2415
                     /locus_tag="PKK36_07895"
     CDS             1117..2415
                     /locus_tag="PKK36_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR94515.1"
                     /translation="MAKAKVSVLRTSPATVLADYHRLMNLAGYQDFLPKDKETALKIN
                     ISWHFFYPGSSTTPWQLDGVIRALKKDGYDPARIHGCHNRTVVIDAHLGERENKQVNV
                     TDHHGLKNVHLYEGEDWINVREAVGDLADKFLCLNQVYPDGFMIPRRFIGENIIHLPT
                     VKTHVFTTTTGAMKNAFGGLLNEKRHWTHPVIHETLVDLLMIQKKIHSGVFAVMDGTF
                     AGDGPGPRCMIPHIKNVLLASADQVAIDAVAAKLMGFDPLSIKFIRLAHDKGLGCGDP
                     REIEITGDEEAARENWHFTGPFKKMTFASRMQHAIYWGKLKKPIEWSLKTILAPWAYI
                     ASVLYHDMFWYPMHQRRVHKILGSEWGRLFQNWDEKAMPPEDLAAPGWTDVGTAPAEM
                     KTESMKLFGRSLKILGTCIKEAPEFAAKKRHKDEKRKKEH"
     gene            2390..2761
                     /locus_tag="PKK36_07900"
     CDS             2390..2761
                     /locus_tag="PKK36_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94516.1"
                     /translation="MKSEKKNIEVRCPACGRETLLLKKPLYEGFTRVGEELSCTLCGH
                     VFSGEESVESVERSGPEIFSDDDRPAEVEIFGEEDAPAFCRQCEHYVVNPFMQWCAFH
                     KQKVEATDTCRDFRRAEKNEP"
     gene            2758..4182
                     /locus_tag="PKK36_07905"
     CDS             2758..4182
                     /locus_tag="PKK36_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNR94517.1"
                     /translation="MRKVFCLIFLAALAAGAAAADISASAPQGITLTVYDTGLALARD
                     LRRVDLPRGEVSVRFAQLPRTIQPETLSFSPPPNVSGIDVREQQVVYDLDSLQSMMRR
                     YESRLLTVTTPAGTESGVLQPMQDTSGAFALRKNDGSVSVIPADKVMAVTFPDAGTQA
                     FLEPALLWRAVVSTEGPQGIRLSYGLSDLSWRAAYELIMEPGGTAGSLSGRAGILNRS
                     GADFSDARVRLVATAGRGGADSAAALRYAYGWRHPSAGAAATFPAPTGSFDLPQPVTL
                     KNNEEKFVTFCSAPSIPVSRFYVYDGVQFDRFQRNRRNDWNYGTEYQTVVDQRLEFEN
                     KEQYGLGFGLLPGVFRLYERRGDGSVDLAGENRMDEVPAEGKGSVLLGPARGLRGERE
                     RTGYSEVVPSRAYEETFEIRLENDTASEVEIRVVEHLYRWNNYEIVRADAEYTETAPQ
                     TIEFRPILKPGGRRAIHYTVRYSW"
     gene            4182..5441
                     /locus_tag="PKK36_07910"
     CDS             4182..5441
                     /locus_tag="PKK36_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94518.1"
                     /translation="MHFRSSIRLAAMCSLVAASAIAEEPVTLAVGPASTLVQEWRTLV
                     LQRGEQEIEVLNLPAETDMSSLQLRLKRRALPVLSWSAGEAPAPDGFSRRGRDARWSR
                     SGSGDSAAGDLQTVRLRVNSPSSGRQDVQLIYLVSNITWSASYQVAVRGEVADDRPVS
                     VDLWSRITLVNSTALAFDRARLYLTGLPPAPPAKKPPGFLMLNRESPLSDLWRPAVPR
                     PEPQYTYEIPEPVRIMPGRSDIKFTVAERLPALPLYRLTSEAVPVSTRRKGWPLELLL
                     VLRNRRASGLGVALPPGPVDIHRGSLWGPLTDRGWLPHTPVDGEMRISLGASEDVQGW
                     RRDLGRQRADVDAWHVDYEIEIQNLRKSPVMIEVEEAPPVVLAWELFSSTESCVRRAG
                     RLIFRQEVPEESRHRIEYRLHVREAAL"
     gene            complement(5484..6077)
                     /locus_tag="PKK36_07915"
     CDS             complement(5484..6077)
                     /locus_tag="PKK36_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94519.1"
                     /translation="MKNWFFVLAFVCFSAAAASAQINMDWTYHDDADDVDDRATEQVP
                     GEDFTFLSPSGIVIPNDAPVVIYVLTANNFAGDLDEQVFVRWWNGETEHWVMAQWIKN
                     IPLSPMGLNAQTFHGLPLAGEAMLDLWRVEIGADITRPGENYYVIQAKGWKDGDTKEA
                     YLLRDAVPEKSQENALGQQWIGTPDYAGHDWSVRIGN"
     gene            6231..7256
                     /locus_tag="PKK36_07920"
     CDS             6231..7256
                     /locus_tag="PKK36_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR94520.1"
                     /translation="MKRPTISDIAAECGVSLSTVSLVLNNNPRISEATREKVWKAVEK
                     FSYYPNVQARGLAMCASQVLSVVVPNLRHVFSDIYFGEIISGIYDAATQANCKVILDI
                     ANEKFVERHEHMSLLKSRRVDGMLFIASSVEHEYLFDFEKTSYPFMLVNHYFPGHSLN
                     YIVFDYNQSGRISAEHLLGLGHRSIGIITGLNTYTGRSLSESFRTTCIGAGLSEKDLP
                     LTDCGPEWSEGAGEEAARRLVEANPSITALMCGNDRMAIGALRYLHTRGIRVPEQMSV
                     IGMDDIPRATYTTPGLTTIHHSLYEIGRLACERLLSLFRGEIKTCSEVLPVNLVVRES
                     TATPASR"
     gene            7259..7717
                     /locus_tag="PKK36_07925"
     CDS             7259..7717
                     /locus_tag="PKK36_07925"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR94521.1"
                     /translation="MNVTRLLVDGYSLLHSAPEFSKYIRSGMEIGRERVLQRVRLALP
                     DLAPEAVVVFDGREGSLEDMEHIQVVYAPAHKTADAVIERMVAESPHPEELLVITADR
                     AERDTAAGFGAQVMSCAQFLELTGRVRTENMPRPRKKKLGTLGDAFPKPR"
     gene            complement(7810..8805)
                     /locus_tag="PKK36_07930"
     CDS             complement(7810..8805)
                     /locus_tag="PKK36_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR94522.1"
                     /translation="MKGISHFASGVAVTSFFPWAIQAAQESNPMYFILGGAFGLLPDT
                     LDFKIYRFFYKHDLYIEPDPLKPDPQSIADQLAASVKAASESKKLVRVKCSTIRMGAD
                     AWQQYVIKFDTETDEVLVKFGPVVNTGQVPIPGASKMEGEIGRAKIPCKLFQSYDATT
                     RVDIFDGPTFGFASDGKGGVIMHFLPWHREWTHALTVGLFFGIIGTLIWDWRAGAVIT
                     AGYSVHVLEDQLGFMGSNLFWPFTRIRFPGLHWMRSGDAMPNFGTVWLSCLIIFYNLY
                     RAMPSSIYHFNLLHVLFYGAVVPIGLFKLALWFVNRGKVAETGPIELEDEWGDKA"
     gene            complement(8824..10158)
                     /locus_tag="PKK36_07935"
     CDS             complement(8824..10158)
                     /locus_tag="PKK36_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNR94523.1"
                     /translation="MTSALLALFATVVGTVLSCVLSCLPGLHVYNVMGLLVLLIYWLA
                     DLGITMPAEVYLPFMSSMIVGWSILNTIPSMLLGAPDESAIFTVLPGQKYLMMGRGYE
                     GTMMTGVGSLAGIFILVFIVGPLAPKLLPVVQAVLRGHMHWVLWVIITYILLSEWPKG
                     GNSGPAGWRKFGDAWRSLAAGLFTFFISGIMGFILLNRSPISTEMAFQNIMPAFVGLF
                     AIPWCLLNIISGAEVPPQHIADSMDVDGSVILRSSAAGGLGGGFAAFFPVITGGIGGL
                     LAGHATAQRDERVFIMSQGVSKVLYYTGAFMLMFVPGLGVTRGGGAWMIKSLHTPVGY
                     GDYYLIMATIAIAGAVSYLIMSPLTRGVIWLIGRYNYRYVSAVALAIIVLLVYLVTSW
                     AGLFIMAVGTGIGLIPVLFASRRLNCLGILLLPIACNMSGFGHKIADLLGLI"
     gene            complement(10166..>10729)
                     /locus_tag="PKK36_07940"
     CDS             complement(10166..>10729)
                     /locus_tag="PKK36_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94524.1"
                     /translation="PAAGSKAPTRIIIEDGGTYRMVVYWEKVAQQIGNNKPQVGQRIQ
                     VRGDVDVYRDEVQLKVTFPDQITILSAAAPVAAAQMDVDYAGGPADLGEAMPAGQVTT
                     AMEGQVVTVQGVLGEPESLRSGVVYPLTDASGTVKIVLWDRLVAGSARDALDTGITVK
                     VRGPVKPYQGVAQVVPENEQGIAVVQE"
BASE COUNT         2212 a         3169 c         3226 g         2122 t
ORIGIN      
        1 ggctgaatga ccccgtctat ggccaccgag atgtcgtcaa cggtgtcgtt ttccagaaca
       61 agtgttccca tgcctgccgg cggcttgtga tcataatcgt cgtcatcctc acatccggca
      121 aggggaagca gcgctgcggc cagcagcgcg caaaaaatca tgcggcgaaa gatgttttta
      181 tttccggaca tgcttcacca gtttcaggca gttgcggttg tccgggagtt tctcgtaggt
      241 cacctgatcc accgtttttc taatcaggaa cagtccgacg ccccggacat tgccctcgtc
      301 ccagtgatcc gggaacttgg gcagcacatg ggcattgaag ttgaagtgtt ttccgcggtc
      361 gatgatctca atgatgaagt cattgtcatc cacataaatg gcaagattga agctgggatg
      421 cggattcatg ccgctgtagg cgtgatccac cacgttgctg cacgcttcat ccacggccag
      481 ttcgatctcg ccaatccggt tttcgtccat ccccgccatt tcggccagag aggtgaccac
      541 catgcggatg agtgcaagcg aatgggcttc accgggaact tccacttcgc agggtccaag
      601 ggatatcatg agtccgaacc tcctttgtcc gtcgtgccca gggtgtcgat cgcatcctca
      661 agggtgggaa gcaccttgag catggcattc aaccccagca gtttgatgat atgttcgatt
      721 ttcaggggca cgcagcacac caggaatatg ccgccgttgg attcgcaggt gtgatgaaaa
      781 cgataaagca gggcaaaact gcggctgttc atgtaggata tgccgccgca gtccagtatg
      841 atccggctgt ttggggtctc gcaccggggc atgatcaggg agcgaaattc ctccacattg
      901 gaagtatcca cagctccttc aacggaaatc agcgtgaatc cgtcctgatc ttcggcgcga
      961 actgttaggg ggcaggttgt catgaattcc ttttccggcc cgtcgtcggc actaaacctc
     1021 tttcctcata aaggatttca ctcttcacag tttttgtaat atattgatct gaacagtttt
     1081 tgtccagtgc ggctcaaaac aaaaaaggct tggattatgg ctaaagcaaa agtatcagtc
     1141 ctgcggactt caccggccac ggtattggcc gattatcacc ggttgatgaa tctggcggga
     1201 tatcaggatt tcctgccaaa agacaaagaa accgccctga aaatcaacat cagctggcat
     1261 ttcttctatc ccggaagttc cacgactccc tggcagctcg acggggtgat ccgtgcgctg
     1321 aagaaagacg gatatgaccc cgcgcgcatt cacggctgcc acaaccggac cgtggtgata
     1381 gatgcgcacc tgggcgagcg cgaaaacaag caggtgaatg tcactgatca tcatggcctg
     1441 aaaaatgttc atctttatga gggcgaggac tggatcaacg tgcgcgaggc cgtgggagat
     1501 ctggccgaca aattcctgtg cctcaaccag gtctatccag acggattcat gatccccaga
     1561 cgcttcatcg gcgagaacat catccatctg cccaccgtca agacccatgt cttcacaacg
     1621 accaccggcg ccatgaagaa cgccttcggc ggtctgctga acgagaagcg gcactggacc
     1681 catccggtta tccatgaaac actcgtggac ctgctgatga tccagaagaa gattcattcg
     1741 ggcgtgttcg ctgtcatgga cgggactttt gccggtgacg gccccggccc gcgatgcatg
     1801 atcccgcaca tcaaaaacgt cctcctggcc tcggccgatc aggtggccat tgatgccgtg
     1861 gcggcaaagc tgatgggctt tgaccccttg agcatcaagt tcatccgtct cgcgcatgac
     1921 aaggggcttg gctgcggcga tccgcgcgag attgaaatca ccggggatga ggaggccgcg
     1981 cgcgagaact ggcatttcac ggggcccttc aagaaaatga cctttgccag ccgcatgcag
     2041 cacgccatct actggggaaa actgaaaaag ccgattgagt ggtcactgaa aactattctt
     2101 gcgccctggg cctatattgc cagtgtgctg tatcatgaca tgttctggta cccgatgcac
     2161 cagcgccgcg tgcataagat actgggcagc gaatggggcc gcctgtttca gaactgggac
     2221 gaaaaggcga tgcctcctga agacctggcc gctcccgggt ggacggatgt cggcacggcg
     2281 cctgcggaaa tgaagacgga aagcatgaag ctgttcgggc ggtcgctcaa aattctcggc
     2341 acgtgcatca aagaggctcc cgaatttgcc gcgaaaaagc gccacaagga tgaaaagcga
     2401 aaaaaagaac attgaggtcc gctgtccggc ctgcggccgc gaaaccctgc tgctgaaaaa
     2461 gccgctctat gaagggttca ctcgcgtggg cgaggagctg agctgcacgc tgtgcggcca
     2521 cgtgttctcc ggggaggaat cggtcgaatc tgttgagcgc tccggtccgg agatattttc
     2581 cgatgacgac cgtcccgcgg aggtggagat tttcggcgag gaggacgcac ccgcattttg
     2641 caggcagtgt gagcattatg tggtgaatcc cttcatgcag tggtgcgcgt ttcacaagca
     2701 gaaggtggag gcaacagaca cctgccgcga tttccggcgg gcggagaaaa atgaaccatg
     2761 agaaaagtat tttgtttgat ctttcttgcg gccctcgcgg ccggcgcggc agccgcggac
     2821 atcagcgctt ctgcgcccca ggggataacc ctcactgttt atgacacggg acttgcgctt
     2881 gcgcgtgacc tgcgcagggt ggatctgccc cggggcgagg tctctgtccg tttcgctcag
     2941 ctgccgcgaa ccattcagcc ggaaacgctt tccttctcgc ctccgccgaa tgtctccggc
     3001 attgatgtga gagaacagca ggtggtttat gatctggatt ctctgcagtc aatgatgcgc
     3061 cgctacgaga gccgtctgct gaccgtgacg acccctgcgg gcacggaaag cggggtgctg
     3121 cagcccatgc aggacacgtc cggagccttc gccctgcgca agaacgacgg atccgtttcc
     3181 gtcattccgg cagacaaggt catggctgtc acgttccccg atgcgggaac ccaggccttt
     3241 ctggaaccgg cgctgctgtg gcgcgcggtg gtcagcacgg aagggcccca gggaatccgt
     3301 ttgagctacg ggctgtccga cctgtcgtgg cgggccgcct acgagctgat aatggagccc
     3361 ggcggaaccg ccggcagtct cagcggccgc gccggcattt tgaaccgttc cggcgccgat
     3421 ttttcagatg cgcgggtccg gctggtggcg accgccggcc ggggtggagc ggattctgcc
     3481 gctgcgctgc gttatgcgta cggatggcgc catccctcgg ccggcgccgc ggcgacattc
     3541 ccggctccaa ccggatcttt tgatctgccc cagcctgtaa ccctgaaaaa caatgaggaa
     3601 aaatttgtaa ccttctgctc tgcgccgtcc atcccggtca gccgttttta tgtctacgac
     3661 ggtgtgcagt ttgaccgctt tcagcgcaac cggcgcaacg actggaacta cggcacggaa
     3721 tatcaaaccg tggtggatca gcgccttgag tttgaaaaca aggaacagta cggcctgggc
     3781 tttggcctgc ttcccggcgt cttccgcctg tatgagcgtc gcggtgacgg ttctgtggac
     3841 ctggcggggg aaaaccggat ggacgaagtg cccgcagaag ggaagggaag cgtcctgctg
     3901 ggtcccgctc gcggcctgcg cggcgagcgt gagaggacgg gctacagcga agtggttcca
     3961 tcgcgcgcgt atgaggaaac ctttgaaatc cggctggaaa acgacaccgc ttccgaggtt
     4021 gaaatccgcg tggtcgagca tctgtaccgt tggaacaact atgaaatcgt gcgggctgat
     4081 gcggagtaca cggagaccgc cccgcagacc atcgagttcc gcccgatcct gaagccgggc
     4141 ggccgccggg ccattcatta cacagttcgc tacagctggt gatgcatttc cgctcatcca
     4201 tccgccttgc cgcgatgtgc tccctggtgg ccgcatcggc catcgcggaa gagccggtca
     4261 cgctggccgt cgggcccgcc tccacgctgg tgcaggaatg gcgcactttg gtcctgcagc
     4321 gcggtgaaca ggaaattgaa gtcctgaatc ttccggcgga gaccgatatg tcctcgctgc
     4381 agctgcgcct caagcgccgc gcccttccgg tgctttcgtg gagcgccggg gaagcccctg
     4441 cgccggacgg tttctcgcgc cgtggccgcg atgcccgttg gagtcgttcc ggatccgggg
     4501 attctgccgc aggcgatttg caaaccgtcc ggcttcgggt gaattccccg tcatccggga
     4561 ggcaggatgt acagttgatt tatctcgtca gcaacatcac atggtccgcg agctatcagg
     4621 ttgcggtacg gggagaagtc gccgacgacc ggcccgtcag cgtggatctc tggagccgga
     4681 tcacgctggt gaattccacg gcgctggcat ttgaccgggc ccgcctgtat ttgaccggcc
     4741 tgccgcccgc tcctcccgcg aaaaagccgc ccgggtttct gatgctgaac cgtgaaagcc
     4801 cgctgtccga cctttggcgg ccggctgtgc cgcgtcctga gccgcagtat acctatgaaa
     4861 ttccggaacc ggtgcggatc atgccgggcc ggtccgacat caaattcacc gtggcagaac
     4921 gtctgccggc tcttcctctt taccggctga cttcagaggc ggtccccgtc agcacgcgcc
     4981 gcaaaggctg gccgctcgaa ctgctgctgg tgctgcgcaa ccggcgtgcg tcgggactgg
     5041 gggtggcgct tccgccggga ccggtggata ttcatcgagg gagtctctgg ggccccctga
     5101 ctgaccgtgg atggcttccg cacacgccgg ttgacggcga aatgcggatc agcctcgggg
     5161 ccagcgagga tgtgcagggc tggcggcgcg atttggggcg gcagcgtgcc gatgtggatg
     5221 cctggcacgt ggactacgaa attgaaatac agaatctgcg gaagtcgccg gtcatgattg
     5281 aagtggagga ggcgcctccc gtggtgctgg cttgggaact cttcagttca accgaaagct
     5341 gcgtccgccg cgccggccgg ctgattttcc gtcaggaggt tcccgaagaa agccgccatc
     5401 ggatagagta tcgactgcat gttcgcgagg ccgcgctgta gcgtcttgta attccggccc
     5461 gcgcggtttc ccggggaagg ccgtcagttg ccgatgcgga cggaccagtc gtgcccggca
     5521 taatccggtg ttccgatcca ctgctggcca agggcatttt cctggctctt ttcgggcact
     5581 gcgtcacgga gaagataagc ctctttggta tcgccgtctt tccatccctt ggcctggatc
     5641 acatagtaat tctcaccggg ccgcgtgatg tccgcgccga tttcaacgcg ccacaggtcc
     5701 agcatggcct caccggccag aggcaggccg tgaaaggtct gcgcgttgag acccatcggc
     5761 gagaggggaa tattcttgat ccactgtgcc atgacccagt gttcggtctc gccgttccac
     5821 cagcggacaa acacctgttc atcgaggtcc cccgcaaaat tgttggcggt gaggacatag
     5881 atgacgacgg gcgcatcgtt ggggatgaca atgccggagg gggacaggaa agtgaaatct
     5941 tcgccgggca cctgctctgt ggcgcgatca tccacatcgt cggcgtcatc atgataggtc
     6001 cagtccatgt tgatctgcgc ggaggcggcc gcggcggaaa agcagacaaa ggccaaaacg
     6061 aaaaaccagt tcttcatgac acgcatcctt tcatgcaaaa agcgttcaag gcctttatta
     6121 aaacgttaaa gccttccgga aacaagaaaa tagcgggcgg ctaaaccgct tgacttcgcc
     6181 ggctgtttgt tggaaccttc cccggtaaat ttttttgaac aggattgatc atgaaacgcc
     6241 caacaatcag tgatatcgcc gcggagtgcg gagtttccct cagtacggtt tctctggtgc
     6301 tcaacaacaa tccccgcatc agcgaggcca cccgcgagaa agtctggaaa gcggttgaaa
     6361 agttcagcta ctatcccaac gtccaggcgc gcggtctggc catgtgcgcc agccaggtcc
     6421 tcagcgtggt ggttcccaac ctgcgccacg ttttctctga catctacttc ggagaaatca
     6481 tcagcggcat ctatgatgcc gccacacagg ccaactgcaa agtgattctg gacattgcca
     6541 acgaaaaatt cgttgagcgc catgagcaca tgagcctgct gaaatcgcgc cgcgtggacg
     6601 gcatgctttt cattgcatcg tccgtggagc atgagtatct ctttgatttc gagaaaacct
     6661 cctatccctt catgctggtc aaccattatt tcccgggaca cagtctcaac tatatcgttt
     6721 ttgactacaa ccagtcgggc cgcatctccg cggagcatct gctgggtctc ggtcaccggt
     6781 ccataggaat catcacgggc ctgaacacgt acaccggccg cagcctgagc gaaagcttca
     6841 gaaccacctg tattggggcc ggcctgtcgg aaaaggatct tccgctgacc gactgcggtc
     6901 cggaatggag cgaaggcgca ggcgaggagg ccgcccgccg gctggttgag gccaacccct
     6961 ccatcactgc gctcatgtgc ggaaacgacc gcatggccat cggcgcgctg cgctatctgc
     7021 acacccgcgg catccgggtg ccggagcaga tgtcggtgat cggcatggat gatattcccc
     7081 gcgcgaccta cacgacgccg ggactgacca ccatccacca ttcgctttat gaaatcggcc
     7141 ggctggcctg cgagcggctt ctgtcgctgt tccgcggcga gatcaaaacc tgcagcgaag
     7201 tgcttccggt gaacctggtc gtgcgcgaat ccaccgccac gcccgccagc cgctgagcat
     7261 gaacgtcacg cgcctgctgg tggacggcta cagcctgctt cacagcgctc ccgaattctc
     7321 caaatacatc cgcagcggca tggagatcgg ccgcgaacgg gtgctccagc gggtgcgtct
     7381 ggcgctcccg gacctggcgc cggaggcggt tgtggtcttt gacgggcgcg aaggttcgct
     7441 ggaggatatg gagcacattc aggttgtgta tgctccagcg cacaaaaccg cggatgccgt
     7501 gattgagcgc atggtggcgg agtccccgca ccctgaagag ctgctggtga tcacggctga
     7561 ccgcgccgag cgcgacaccg cggccggttt cggggcgcag gtcatgtcct gcgcgcagtt
     7621 tctggaactc accggacgcg tgcgaacgga aaacatgccg cgcccgcgga agaaaaaact
     7681 gggaacgctg ggcgacgcgt ttccgaaacc ccgctgatca aaaacttcca atccctggaa
     7741 cttttctcaa aaaaacttcc aatcattgga actttttgcg gaaaaacttc caatgactgg
     7801 aacttttgct caggccttgt cgccccattc gtcttcaagc tcaatcggtc cggtttccgc
     7861 gactttgccg cggttgacga accaaagcgc cagcttgaac aggccgatgg ggaccaccgc
     7921 gccgtagaaa agcacgtgca gcaggttgaa gtgatagatc gaggacggca tggcccggta
     7981 caggttgtaa aagatgatca ggcagctcag ccatacggtg ccgaagttcg gcatggcgtc
     8041 gccggatcgc atccagtgga gaccggggaa cctgattctt gtgaagggcc agaacaggtt
     8101 cgatcccatg aatccaagct ggtcctcgag cacatggacg gaatagccgg cggtgatcac
     8161 ggcgcccgcc cgccagtccc agatcagcgt gccgatgatg ccgaaaaaca gccccacggt
     8221 cagagcatgc gtccattcac ggtgccacgg gaggaagtgc atgatcacac cgcccttgcc
     8281 gtcggaggca aacccgaacg tcgggccgtc gaagatatcc acgcgggtag tggcgtcata
     8341 actctggaac agcttgcagg ggatcttcgc gcgtccgatt tctccctcca ttttgctggc
     8401 gcccgggatg ggcacctggc cggtattgac cacgggcccg aacttgacga gcacctcgtc
     8461 tgtttccgtg tcgaacttga tcacatactg ctgccacgcg tcggccccca tgcggattgt
     8521 gctgcacttc acccgcacca gctttttact ttcagaggcg gccttgacgg aggccgcgag
     8581 ctggtcggcg atgctctgcg gatcgggctt cagcgggtcc ggctctatat acagatcgtg
     8641 cttgtaaaag aagcggtaga tcttgaaatc gagcgtgtcg ggcagcaggc cgaaggcgcc
     8701 gcccaggatg aaatacatcg gattgctttc ctgtgcggcc tggatcgccc aggggaaaaa
     8761 agaagtgacg gccactccgg atgcgaaatg tgaaatacct ttcatggctt cagttccttc
     8821 cgtttatatc agtcccagca ggtcggcaat tttgtgcccg aagcccgaca tgttgcaggc
     8881 gatcgggagc agcaaaatcc ccaggcagtt caagcggcgc gacgcgaaga gcaccggaat
     8941 cagtccgatg cccgttccca ccgccatgat gaacaggccc gcccagctcg tgacaagata
     9001 caccagcagc acgataattg ccagtgcgac ggccgagaca tagcggtagt tgtagcggcc
     9061 gatcagccag atcacgccgc gggtgagggg gctcatgatg aggtaggaga ccgcgcccgc
     9121 gatggcgatg gtggccatga tcaggtaata gtcgccatag ccgacggggg tgtgcaggct
     9181 cttgatcatc caggcgccgc cgccccgggt gactccgaga cccggtacaa acatcagcat
     9241 aaacgctccg gtgtagtaaa gcaccttgga gacgccctgc gacatgatga acacccgctc
     9301 gtcgcgctgg gcggtggcgt ggcccgccag caggccgccg atgccgccgg tgatcaccgg
     9361 aaagaacgcg gcgaagccgc cgcccagtcc gcccgcggcg gaactgcgca gaatgacgct
     9421 cccgtccaca tccatggaat cagcgatgtg ctgcggggga acctccgcgc cgctgatgat
     9481 gttcagcagg caccatggaa tggcaaacag gccgacaaag gccggcatga tgttctggaa
     9541 ggccatttcc gtggagatcg gcgaacggtt cagcaggatg aatcccatga tgccggaaat
     9601 gaagaaggtg aacaggcccg ccgccagcga gcgccaggcg tcgccaaact tccgccagcc
     9661 cgcaggacct gaattgccgc ccttcggcca ttccgaaagc agaatatacg tgatgatgac
     9721 ccacagcacc cagtgcatgt ggccgcgcag gacggcctgc acaaccggca ggagtttcgg
     9781 ggccaggggg ccgacaatga agaccagaat gaaaatgccg gccaggctgc cgacgccggt
     9841 catcatggtg ccttcataac cgcgtcccat catcaggtat ttctgtcccg ggagcacggt
     9901 gaaaatggcg ctctcgtccg gcgcgcccag cagcatcgaa gggatggtgt tcagaatgga
     9961 ccatcccacg atcatgctgg acatgaaggg gagatagact tccgcgggca tggtgatgcc
    10021 caggtccgca agccagtaga tcagaagcac cagcaggccc atcacgttgt agacgtgcag
    10081 gcccggcagg caggacagca cgcatgagag cacggtgccg acgactgttg cgaataaagc
    10141 gagcagggcg gatgtcatgt ttgacctatt cctgcaccac ggcgatgccc tgttcgttct
    10201 cgggaacgac ctgtgcaacg ccctgatagg gcttcacggg tccgcgcacc ttcacggtga
    10261 tccccgtgtc cagcgcgtcg cgggctgatc ccgccaccag ccggtcccac agcacgatct
    10321 ttacagttcc gctggcgtcg gtcagcggat aaaccacgcc gcttcgcagc gattcgggct
    10381 ctccaagcac accctgcacg gtgaccacct gaccctccat cgcggtggtg acctgcccgg
    10441 cgggcatggc ctcgccgaga tccgccggcc cgccggcgta gtcaacatcc atttgcgcag
    10501 ccgccacggg cgccgccgcg gagagaatgg tgatctggtc cgggaaggtg accttgagct
    10561 gaacttcatc gcgataaaca tccacatcgc cgcgcacctg aatgcgctgc ccaacctgcg
    10621 gtttgttatt cccgatctgc tgcgcgacct tttcccagta gaccaccatg cggtaagtgc
    10681 cgccgtcctc gataatgatt cgggtggggg ccttggagcc cgcggcggg
//
LOCUS       DAOQWR010000089        10461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6822_length_10461_cov_4.902556, whole genome shotgun sequence.
ACCESSION   DAOQWR010000089 DAOQWR010000000
VERSION     DAOQWR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6822_length_10461_cov_4.902556"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(644..1330)
                     /locus_tag="PKK36_07945"
     CDS             complement(644..1330)
                     /locus_tag="PKK36_07945"
                     /inference="COORDINATES: protein motif:HMM:NF022958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCL family protein"
                     /protein_id="HNR94525.1"
                     /translation="MKKTRRVSYCFGAYSFTSQAAVKKHAAAIRSRYPVGVTISDAAD
                     VAFLMDLINCHAECKTKIGCGISRFYVDNAPDHPGQCFWIERTDHTSTDWGVPSCLIG
                     IGRLNRLSLRMAIKPQMDEFKTKALAQCTETFVSEYSGKKFPVDEAVADHVTEFEDIV
                     QQFFHTQGIDIERELLTMSIDQKSEPVWRDPALLGEFLDYHKKFPLRLVQWRENLSDI
                     KIEEADKSLG"
     gene            complement(1429..4248)
                     /locus_tag="PKK36_07950"
     CDS             complement(1429..4248)
                     /locus_tag="PKK36_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94526.1"
                     /translation="MTELSVKELDLLQRVGEKPELRSIFFRKVKGLKWFNALEGKGYF
                     NPENNPKPQPAEEMGYYTVPHWHAINYLVNTAAELDNDASHAYAVKFLNVLLEATAHA
                     KTNAFSNYHTWWQFAKIISYIPNQVISTENMAVIDYWLDDKFNQSLVAQEVGCGWLPK
                     LLNINDEHAVALSLELLRILYKPVCDLRRSENQGRKKYILRYDSYHSDNITNRTAKIA
                     GARGGKAVISIFESYLTGILRESGSDTWSSIWHPSIEDHEQNRYRTDAENVLIKTYRE
                     SLSGCIEKIPQEACELLTTMLDSEYQTIHRIAIHAISEHQYLQPRFIDRILNAQYLKS
                     NYRHEMWQYLKRNYSQFSDTQKRVVIEMISALSSIEKDGKLNKGATAYTQAVWFAAIK
                     DYGPVESGLYQKNVEMAGVEPDHPDFSSYMSSGTFKDTSSVSLDDLRSLPIDELVAAL
                     QIKEKDGENSARRMGDLSGVLKQLIKANANIYFNKLDNFISLDHAFIYEILNAYREMW
                     VERYGLPWIIIWPCLLKFCSDVISRESLWTSSSSQNIGPFRVGCSQIIGSIAQLLESG
                     TKEDDHVIPDNCLNDVEGIIVTLLKRIKGDHFEEGCDAVSVSINSPRGQCLEALINLT
                     LRQCRISDGVNNGKHIETWTHFCPYYDEELLRAEAPEPEYEFATLVTLYLANFLYMSK
                     EWTVQNLNRIFDQKNYSKWLCAMRGYSYIRGIYPEIYDHLKANGDYIKALDDKNISDD
                     VCEAIIQNICVAYIANYETYESDNSLIKTLIERSVSEEIHHLIWFFWAKRDRGKGDLE
                     NKVFELWPHLIRNVDMTSKEGKKRASELCHWVTFVEEINDDNLPLIQAIAPYADVSHN
                     GYELLEYLAKLSAKQPYEASDIWLKMLDQSGPDYPEDAIKTILHNLIITGAEGLRRAN
                     DVVSKYLKYGAERPAVLLKAMINHE"
     gene            complement(4241..5170)
                     /locus_tag="PKK36_07955"
     CDS             complement(4241..5170)
                     /locus_tag="PKK36_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNR94527.1"
                     /translation="MKHIPDLRESFDPPPEIIEAGLNGELILFVGAGVSRLLALPSWK
                     ELAAQVLEELRQARFINYSELEQLRTLTPKIQMSIADLLAHDNQYALDITKYLKDRNI
                     SGTIYESINNIGCSCVTTNYDELIAPRSIEPVDDSTTSVDIRRISDRNDIYANLLNEP
                     GTVIHLHGSISKPETMVVTTKEYLELYDHKNIQEFLGEMFEKMTILFLGYGLEETEIL
                     EHILRRGSAKANCERKRFVLQGYYLSQVPLYYQLYRYYEHSFGVHLLGYVKDYENHKC
                     LEKIIKSWVEKIQIRRQPLVKDIDFMIEVLGDD"
     gene            5707..6081
                     /locus_tag="PKK36_07960"
     CDS             5707..6081
                     /locus_tag="PKK36_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94528.1"
                     /translation="MKKLTLVMLAGCIAGAIFCMPAQAGEMEKKIKARQAIEQTYLDV
                     LGRKATADDINHYMKLLQEGKDAGKIRKGIILSEECKNAINKIFKEVKGHEADEKNLN
                     KAREALAEGKTLQHIKAKLQKN"
     gene            6081..7286
                     /locus_tag="PKK36_07965"
     CDS             6081..7286
                     /locus_tag="PKK36_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cycloartenol synthase"
                     /protein_id="HNR94529.1"
                     /translation="MRSPIRRIFTLAVLLPGALLAADGKPAVLQSRTSASREISLSLA
                     NEARAAIDRGTQWLLSKQQEGGHWSNPGFPALTALPLWALAGAQCSDTRAIDRAVAYI
                     LACVHDNGAIYREPDQPLKGGGLSNYNTAICMVALHLTGRPEVNPVVLKARQFVARSQ
                     HLGGDIYRGGMGYDEATGRPYADLSNSYIAYEAMRLTEGAEDFRKKGEKKSDLDWDAA
                     RAFVQRVHNDADFNTQSWAAADADNKGGFAYHPEQTRAGTTTNAEGVVTFRSFGSMTY
                     AGLLSYIYAEVDRDDPRVKSTVDWAVRHWTLEENPGTGAEGLFYFYNVLAKGLAAYGR
                     DEFTGPDGNSFNWRAEFIRKLLSLQKTDEAGRGYWVNDVGRYWESDPVLVTSYSLIAL
                     EIALGEAGH"
     gene            7349..7702
                     /gene="ssrA"
                     /locus_tag="PKK36_07970"
     tmRNA           7349..7702
                     /gene="ssrA"
                     /locus_tag="PKK36_07970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(7750..9576)
                     /gene="pepF"
                     /locus_tag="PKK36_07975"
     CDS             complement(7750..9576)
                     /gene="pepF"
                     /locus_tag="PKK36_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNR94530.1"
                     /translation="MVTDKKKEKTDSGVPERSDARREDTWDLERIYAEESAWQRDFDS
                     LASLAEPLMAMRGKLGSAAALAEMFHASETLDRLSDKLYVYSHLKADEDTANTSNQAR
                     QSRIMQRLTQISGDLAWITPEILSHTEDELRAWMEDTVLQPWRYVMEKLVRAKPHVLS
                     PPEETLLSRAGEIFSAPRQTYKMLTDADMRFPEVENSRGEKRELTDGRYSTFLISRDR
                     SERQNAFRAMYNTYSSCKNTLASTLAANVKLHNYMAQIRHHESALHAALHPDQIPVVL
                     YESLLKGVHDALPRFYDYVELRREALNLPDLNMYDFYVPLVPEYDIKVPFDQACEWVL
                     ESCRPLGAEYCGILKTAFADRWIDVYENRGKRSGAYSSGCYDTLPYVLMNYQGTLNHV
                     FTLAHELGHSMHSWLANHAQPHQYASYPIFIAEIASTLNEELLLKRLLETTSDPAFRA
                     YLLNHLCDAFKGTVYRQTMFAEFEKMIHELDAAGEPLTFDVLNSRYFELNAKYYGPGI
                     KADPLIAHEWSRIPHFYYNFYVYKYATSFCASQIFISRIYESADGVEKYLGLLKAGGS
                     RPPLELIRKAGVDLENPQTLTGAFDNFGRTVQELRAELKKFQ"
     gene            complement(9570..>10461)
                     /locus_tag="PKK36_07980"
     CDS             complement(9570..>10461)
                     /locus_tag="PKK36_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNR94531.1"
                     /translation="QGSLALNWRASAENEELTSESLGDHSRSRGALQLIPEWEISALR
                     IHAGVRAEVFSGEGPAWLPQAGISIEPVENMTAYLAYTETVRQPSFTELNYESPGSLG
                     NEGLDRQQAGSWEGGVVRQWPEGGYVRAVLFERSSSHTVDWVKPDSPTNRWTATDLGR
                     VKTRGLETEMSCPLTGSLTLHGFYSLLDTESDFEAYSGRYVLDYPEHRLRGGLAWDVL
                     KTVQMNFVQEYRRQTENPERTTGRDGYASEINVTWSPGRLEGLSLSLAADNVWDDDFQ
                     TLAGQKAAGRQVYFAMEMIW"
BASE COUNT         2532 a         2744 c         2540 g         2645 t
ORIGIN      
        1 ttgtcttcaa gttgcgctga aagatgcccc gcggcttgcc gcggggagtt tcactttgca
       61 tcagcgtttc tcacagattc accagcgccc cctttgtcta aaattttctg agcgagaatg
      121 gataacgctg cgaggccccc tgcccacttt cacacccccg cccccctgtc tgttcagact
      181 ggatggattt atgtttcgag atcctgcatc ggcctcattt ggcttcaccg atggtatcaa
      241 tgaacgcgga ttgacgtttt ttggaaggat caaggcaaag aagttcctga tacactatca
      301 gagaatgcgt ttataagccc tctgaggccc aaattgaggc ccaggacgtg tgttgggcat
      361 ttccagccct tttcaggccc tacgcggcca tttcagagca tttggaggca ttgggaacag
      421 catgacgccg tcccttttgt cccggcgtcg ccggctatga ttccattcgc cacgatccca
      481 agctgtcttc tacttgtttt agctatatct atactttgcc gggacggggc caagcccaag
      541 ccgcagccat tctgatctca gacccagtca tcaactggga aaaataatgg atgccgtttc
      601 cccgcaaatt gcaaaaatcc gcccgccaat ggatcgccac ccatcacccg agacttttgt
      661 cagcctcttc tattttgata tcgctcaaat tctctcgcca ctgaacaagg cgcaacggga
      721 acttcttgtg atagtcaaga aactccccta aaagcgctgg atcacgccac acaggttcac
      781 tcttctggtc tatgctcata gtcagcagtt ctcgctcaat atcgattcct tgggtatgga
      841 agaattgctg gacaatatcc tcaaattctg tgacgtgatc ggcaacggct tcatctactg
      901 ggaacttctt cccggaatac tccgacacaa aagtctcagt acattgcgca agcgcctttg
      961 tcttgaattc atccatctga ggtttaattg ccatgcgcaa tgacaaccgg ttcagtcgac
     1021 caatcccaat aagacatgaa ggaacgcccc aatccgtcga agtgtgatca gtgcgctcta
     1081 tccagaaaca ttggccagga tgatccgggg cattgtcaac gtagaaacga gaaataccgc
     1141 atccgatttt agtcttacat tcggcatgac agttaatcaa atccattaag aatgccacat
     1201 ctgcggcatc cgatatagtg acaccaacag gatatcgaga tctaatggct gctgcgtgtt
     1261 tcttgaccgc agcttgtgaa gtgaatgagt atgcgccgaa acagtagctt acacgtcttg
     1321 tctttttcat ccgcagttga aggttgtaat caacgattcg tgtgactgta gtcgctggct
     1381 ttgtctcgct gaaatatatt ggccattccc ccccacacat ctttatattt attcatgatt
     1441 aatcatcgcc ttcaacaata cggctggacg ctcagcaccg tatttgaggt acttactcac
     1501 tacatcattt gcccggcgca atccctcagc acccgtaata atcaaattgt gtaatattgt
     1561 ttttatcgca tcttctggat aatccggacc cgattgatca agcatcttca gccagatatc
     1621 cgaagcctca taaggctgtt tcgcgcttag tttggcaaga tattccaaca attcatagcc
     1681 attatgagat acatccgcat aaggcgcaat cgcctgaatc aacggcaggt tatcatcatt
     1741 gatttcctcg acaaaagtca cccagtgaca cagttcagaa gccctctttt tcccctcttt
     1801 tgaggtcata tcaacgttcc taatgagatg gggccacaat tcaaacactt tattttctaa
     1861 atcccccttc ccccgatccc gttttgccca gaaaaaccag atcagatggt gaatctcttc
     1921 agacacagat ctttcaatca aggtctttat tagactgtta tcactttcat aggtttcgta
     1981 atttgcaatg taggcaacac agatgttctg aattatcgct tcgcatacgt catctgagat
     2041 atttttatca tctaaagctt ttatgtaatc accattggcc ttaagatggt cgtagatctc
     2101 cggataaata cctcgaatat aggagtatcc ccgcatcgca cataaccatt tcgaataatt
     2161 cttctgatca aatattcgat tcaaattctg gaccgtccac tctttggaca tatataggaa
     2221 gttcgccagg taaagagtta caagcgtagc aaattcatat tcaggctcag gcgcctcggc
     2281 tcgcagaagt tcttcgtcat aatatgggca gaagtgcgtc caagtctcga tgtgcttccc
     2341 gttgtttacg ccatctgata ttcggcactg cctcagcgta agattaatca aagcttctaa
     2401 gcattgtcca cgcggactat tgatggaaac agataccgca tcgcagcctt cttcgaaatg
     2461 atcgcccttt atccgcttaa ggagggtgac aataatgccc tcgacatcgt tgaggcaatt
     2521 atccgggata acatgatcat cctccttggt tcccgattcc aacaattgcg caatacttcc
     2581 aataatctga gaacatccaa cacggaaggg gcctatgttt tgggacgagg aagaagtcca
     2641 taaactctcc cgacttatga cgtcagaaca gaacttaagt aaacacggcc aaatgataat
     2701 ccacggtaat ccataacgct ccacccacat ctctcgatag gcattaagta tttcatatat
     2761 aaatgcatga tctaagctaa tgaagttgtc tagcttgttg aaataaatgt tagcatttgc
     2821 tttgatcaat tgtttcaata cgccagacaa atcccccatc cgtcgcgcac tgttttctcc
     2881 atccttctct tttatctgta aggcagcaac aagctcatct atcgggagcg accgcaaatc
     2941 atccagtgac acggaagatg tgtctttaaa tgtccccgat gacatatagc tagagaagtc
     3001 cggatgatcc ggctcaacgc cagccatttc aacgttcttt tgatacaagc cactttctac
     3061 cggtccatag tctttaattg cagcaaacca tacggcttgg gtataggctg tcgctccctt
     3121 attcagttta ccatcctttt ctatgcttga aagtgctgat atcatctcaa taacgacccg
     3181 tttctgggtg tcactgaact gagagtaatt cctcttaaga tattgccaca tttcatggcg
     3241 ataattgctt ttcagatatt gagcattcag gatgcgatca ataaatctcg gctgtagata
     3301 ttgatgctcg cttatcgcgt gaattgcaat ccgatgaata gtttggtatt cgctatcaag
     3361 catcgttgtt agaagttcac atgcctcctg cggaatcttt tcaatgcagc cactcaacga
     3421 ctcgcgataa gtcttaatca acacattctc tgcatctgtt cgatatctgt tctgttcgtg
     3481 atcttcaatt gatggatgcc atattgagct ccatgtgtcg cttccggact cccgcaagat
     3541 tcccgttaag taactttcga aaatcgatat aaccgctttc ccgcctcttg cccctgcgat
     3601 ctttgctgtt ctatttgtta tgttatcaga atgatatgaa tcatagcgta atatgtattt
     3661 tttccgcccc tgattctcgc ttcgccgaag atcacaaacg ggtttgtata atattcgtaa
     3721 aagctctaaa gacagggcta ccgcgtgctc gtcatttatg ttcaacaatt tgggcagcca
     3781 tccacaaccg acctcctgag caacaagact ttggttgaat ttatcatcca gccaataatc
     3841 gattactgcc atattctcag tagagattac ctgattaggt atataggaaa tgattttcgc
     3901 gaactgccac cacgtgtgat aattactgaa agcattggtc ttcgcatgcg ccgtggcttc
     3961 aagcaataca ttgaggaact ttacggcata ggcatgagat gcgtcattat ctagttcagc
     4021 ggctgtatta accaaatagt tgattgcatg ccaatgagga acagtgtagt aacccatctc
     4081 ttcagcaggt tgtggtttag gattattctc tggattgaaa tatccttttc cctctaaagc
     4141 attgaaccat ttcagtccct tgactttacg gaagaatata gatcggagtt caggtttctc
     4201 accaacccgt tgcagcaaat ccagctcttt gaccgacaat tcagtcatca cccaacacct
     4261 caatcataaa atcgatgtct tttaccaacg gctgtcttct aatctgaatc ttctcgaccc
     4321 atgatttaat aattttctcc aagcatttgt ggttctcata atctttgaca tagcccaaca
     4381 aatggactcc aaaagaatgt tcataatacc tataaagctg ataatagagc ggtacttggc
     4441 ttaggtaata tccctgcaag acaaaacgtt tcctctcgca atttgcctta gcggatcccc
     4501 ggcgtaagat gtgttccaat atctctgttt cttccaagcc ataaccaagg aacagaatcg
     4561 tcattttctc aaacatttcg cccagaaatt cttgaatatt cttgtgatca taaagttcca
     4621 agtattcttt tgtcgtcact accatggttt cgggtttgct tatacttcca tgcagatgaa
     4681 taactgtgcc tggttcattt aatagattag catatatatc atttctgtcg gagattctcc
     4741 tgatatccac agaagttgtt gaatcgtcaa caggctcaat ggatcgcggt gcaattaact
     4801 catcatagtt tgttgtgaca catgaacaac caatgttgtt aattgactca tagatagttc
     4861 cagatatgtt tctgtctttg agatattttg taatatctag cgcatattga ttgtcatgcg
     4921 ccaataaatc agcaatcgac atttgaattt ttggcgtcaa tgtcctcaat tgctctaatt
     4981 ctgaataatt tatgaaccga gcttgacgca attcctccaa gacttgagct gcgagttctt
     5041 tccatgaagg caacgctagt agccgcgaaa cacccgcacc aacaaaaagg atcagttctc
     5101 cattcaaacc agcctcgatg atttctggtg gtgggtcaaa actttctctt aaatctggaa
     5161 tatgtttcat tataaacttc ttgcgtcagt caattcggtt cactccggca tcatatttaa
     5221 atggttctcc ctataaacag aaagcctcgt tcaattgata aaggtcggga gataaaccca
     5281 tctcaaatcc caaacgtatt ttttcataaa gtgctcattc caagccgaac aatacacaat
     5341 gcatgaacag acggcgcatc atatcacata atcgttttga gcacccagta agatgcgatc
     5401 ataatcaaat aaattatcca aggagtatgg gcagccgttg tggtatcaaa tgtcgcatca
     5461 atccaaaagg gtgaagcaca tctgtttcgt aatcagaatg ataggaataa gcgtgattaa
     5521 tccgtagtca ggtgctctat ccaattgagc taagggtacg ccgatggttt ggcacaatat
     5581 gacagaacgg cccggaatgc aacccccgga ttccattttt ttgaacggtt ttccggctgg
     5641 tgtttcaaac ccttttgagt atgatgacgc gacgttgtga gaggattttg gataaaggag
     5701 atgaacatga agaaactgac acttgtgatg ctggccggat gtattgccgg cgcgatattc
     5761 tgcatgcctg cgcaggccgg cgagatggag aaaaagatca aggcccgcca ggccattgaa
     5821 cagacctatc ttgatgtgct gggccgcaag gcaaccgctg atgacattaa tcactacatg
     5881 aagctcctgc aggaaggcaa agacgccggg aaaatccgca aaggcatcat tctcagcgag
     5941 gagtgcaaaa acgccatcaa caagatcttc aaggaagtca aggggcacga agccgacgag
     6001 aagaacctga acaaagcccg cgaggcgctg gcggaaggca aaacgctgca gcacatcaag
     6061 gctaaactgc agaagaactg atgcgttccc ccatcaggcg tatttttact ctggccgttc
     6121 ttctgccggg cgcattgctt gccgcagacg ggaaaccggc cgtcctgcag agccgcacat
     6181 ccgccagccg ggagatcagc ctgtcgcttg caaacgaggc gcgcgcggcc atcgaccgcg
     6241 gaacacagtg gctgctctcc aaacagcagg agggcgggca ctggtccaat cccggcttcc
     6301 ccgccctgac ggcactgccc ctgtgggcgc tggcgggcgc gcagtgttca gacacgcggg
     6361 ccatagaccg cgcggtggcc tatattcttg cctgcgtgca cgacaacgga gccatctacc
     6421 gtgagccgga tcagccgctg aaaggcggcg gactgagcaa ctacaacacg gccatctgca
     6481 tggtggccct gcatctcaca ggcagaccgg aggtgaatcc ggtggtgctc aaggcaaggc
     6541 agtttgtggc ccggagccag catctgggcg gcgacatcta ccggggcggc atgggttatg
     6601 atgaggcgac cgggcggccg tatgccgatc tctccaattc ctatatcgcc tatgaggcca
     6661 tgcgcctgac ggaaggcgca gaggatttcc ggaaaaaagg tgaaaagaaa tccgatctgg
     6721 actgggatgc cgcccgcgcc tttgtgcagc gcgtgcacaa cgacgcggat ttcaacacgc
     6781 agtcatgggc ggcggcggat gcagacaaca agggcggctt tgcctatcat cccgagcaga
     6841 cccgggccgg aacaacgacc aatgccgaag gggtggtcac cttccgctcg ttcgggagca
     6901 tgacctatgc aggcctgctg agctatatct acgccgaagt ggatcgggat gatccccgcg
     6961 tcaaatccac tgtggactgg gcggttcggc actggacgct ggaagaaaat ccaggcacgg
     7021 gcgcggaggg gcttttctat ttctacaacg tgctggccaa gggcctggcg gcctacggac
     7081 gggatgagtt caccggaccg gacggaaact cctttaactg gcgggccgaa ttcatcagga
     7141 aactgctgag cctgcagaag acggatgaag ccgggcgcgg ctactgggtg aacgatgtgg
     7201 gccgctactg ggaatcagat cccgtgctgg tgacctccta ctcgctcatc gccctcgaaa
     7261 tcgcgctggg cgaagccggc cactgaattc cgcgaaagaa cggttgatat ttcccgccgt
     7321 gtgcgtagat tattagtacc tcattcatgg gggcgactgg gttcgacgtg taggttgaag
     7381 cttcagttgc gtgtcgaggt tggtctccgg gcctcgtaaa aaggagtcca aaaattaaat
     7441 gctgacgcaa gttacgcatt agcggcataa ttagtccgct acgtctctgc cctgacgcct
     7501 gttaggggac agaggcgacg acaacaggct ggcgccaaag ccggggaacc gggtggaggc
     7561 gcgagatcca acaggtttct agcggctgag gtggccctga cagacggcag ccaaggcggc
     7621 gaatctttaa agtctgaata cacacgtaga tactgctgtg actcttatgc ggacgggggt
     7681 tcaaatcccc ccgcctccac cattttgaaa agttccaacc attggaagtt ttcagaccaa
     7741 aagttccaat cattggaact ttttgagttc ggcgcggagt tcctgcacgg tacggccgaa
     7801 gttgtcgaag gctccggtca gggtttgcgg attttccaaa tccacgccgg ctttacggat
     7861 cagttccagg ggcggccttg atcccccggc cttcagcagg cccagatatt tttccacgcc
     7921 gtccgcgctt tcatagatgc gactgatgaa aatctgtgag gcacagaaac tggtggcata
     7981 tttgtagaca taaaagttgt agtagaagtg cggaatgcgc gaccactcgt gcgcgatcag
     8041 aggatcggcc ttgatgccgg ggccgtaata tttcgcgttc agttcaaaat agcggctgtt
     8101 cagcacatca aaagtcagcg gttcgcccgc cgcatcgagt tcgtgaatca ttttttcaaa
     8161 ttccgcaaac attgtctggc gatagacagt gcccttgaac gcgtcgcaca ggtgattcag
     8221 cagataggcc cggaaggccg gatcgctggt ggtctccagc aggcgcttga gcaggagttc
     8281 ctcgttcagc gtggacgcga tttccgcgat gaagatcgga taggacgcgt actggtgcgg
     8341 ctgagcgtga ttggccagcc agctgtgcat cgagtgcccc agttcgtgcg ccagcgtgaa
     8401 gacgtgattg agcgtgccct gatagttcat cagcacgtac ggaagcgtgt cgtagcatcc
     8461 gctggaatag gcacccgatc gcttgccgcg gttttcatag acgtcaatcc agcggtcggc
     8521 gaaggccgtt ttcaggatgc cgcagtattc tgcgcccagc gggcggcagg attccaaaac
     8581 ccattcgcag gcctgatcga acggaacctt gatgtcgtat tccggcacca gcggcacata
     8641 gaagtcgtac atgttgaggt ccggcagatt cagcgcctcg cgccggagtt caacatagtc
     8701 ataaaaccgc ggcagcgcgt catgcacacc cttcagaaga gattcataaa gcacaacggg
     8761 aatctgatcc ggatgcagag ccgcgtgcag cgccgattca tgatgacgaa tctgcgccat
     8821 gtagttgtga agcttcacgt tcgccgccag tgtcgaggcc agcgtgtttt tgcaggaact
     8881 gtaggtattg tacatcgccc ggaaggcatt ctgccgctcg ctgcggtcgc ggctgatcag
     8941 gaaggtggaa tagcggccgt ccgtgagttc gcgcttctcg cccctgctgt tctccacttc
     9001 ggggaagcgc atgtcggcat cggtcagcat tttgtaggtc tggcgcggag cggagaatat
     9061 ttctccggcg cgggaaagaa gcgtctcttc cggcggcgag agcacgtgcg gcttcgcgcg
     9121 gaccagcttt tccatgacat agcgccatgg ctgcagaact gtgtcctcca tccacgcgcg
     9181 cagttcatct tccgtgtgcg acaggatttc cggtgtgatc catgcgagat caccggagat
     9241 ttgcgtgagc ctctgcatga tgcgcgactg gcgcgcctga ttcgatgtgt tggccgtgtc
     9301 ttcatcggcc ttcaggtgcg aatacacgta gagcttgtcg ctcaggcggt caagcgtttc
     9361 ggaagcatgg aacatctctg ccagcgcagc ggccgaaccg agttttcccc tcatggccat
     9421 cagcggttca gccagcgatg ccagcgaatc gaaatcgcgc tgccaggcgg attcctccgc
     9481 ataaatccgc tccagatccc aggtgtcttc ccgccttgcg tctgagcgtt ccggaacgcc
     9541 cgaatcagtt ttttcttttt ttttgtcagt taccatatca tttccattgc gaaatatacc
     9601 tgccgtccgg cggctttctg tccggccagt gtctgaaaat catcgtccca gacattgtcc
     9661 gctgcgagag acaaactcaa tccctccagc cggccggggc tccaggttac attgatttcg
     9721 gaggcgtatc catcccggcc ggtcgtccgc tccgggttct ccgtttgcct gcggtattcc
     9781 tggacgaaat tcatttgcac ggttttcaga acatcccacg caagcccgcc gcgcaggcgg
     9841 tgctcgggat agtccagcac atagcggccg gagtatgcct cgaagtccga ttccgtgtcg
     9901 agcaggctgt agaatccatg cagcgtcagg cttccggtga gcggacagct catttccgtc
     9961 tccagtccgc gggttttgac tcgaccgagg tccgttgcgg tccagcgatt ggtgggcgaa
    10021 tcgggcttta cccaatccac cgtatggctg gaggaacgct caaacagcac ggcgcggaca
    10081 tagccgcctt caggccactg gcgcaccacc ccgccctccc acgagccggc ctgctggcgg
    10141 tcaaggccct cattgccgag acttccggga gattcgtaat tcaattccgt gaaggagggc
    10201 tgacggaccg tttcggtgta cgcgagatag gccgtcatgt tttcgacggg ctcgatcgaa
    10261 atgccggcct ggggcagcca tgccgggcct tcgccggaga agacctccgc ccggacaccc
    10321 gcatgaatcc tgagcgctga gatttcccat tcggggatca attgcagcgc tccgcgcgac
    10381 cgactgtgat caccgagact ttcactcgtg agctcctcgt tctcggcgga agcacgccag
    10441 ttgagggcca gactcccctg c
//
LOCUS       DAOQWR010000090        10411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_6863_length_10411_cov_5.070780, whole genome shotgun sequence.
ACCESSION   DAOQWR010000090 DAOQWR010000000
VERSION     DAOQWR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6863_length_10411_cov_5.070780"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..576)
                     /locus_tag="PKK36_07985"
     CDS             complement(97..576)
                     /locus_tag="PKK36_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94532.1"
                     /translation="MHRSVAEMVDAGGRTAQSFGVSRLFGQIYILLYLSPDPRSLDEM
                     AASLGVSKASVSITCRQLESWGAVKRVWVKGDRKDYYQAQSDLSTIINNGLLSAVNKK
                     LDTAEIQIQRSIELLNDDKAGGENKEFLRRKLVEAEKYRSKAAKLLNNPILKHIFAP"
     gene            773..1387
                     /locus_tag="PKK36_07990"
     CDS             773..1387
                     /locus_tag="PKK36_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94533.1"
                     /translation="MKWFVSLFAAVLTVSLMSGCAMFRASTSDVDLSDTSKHFDADFD
                     ATDMRGITESVAAELLAGPFLQGQAAPPITMIAGIQNRTSNYVDTKNLTDRMRTILLK
                     SGKMQFVNEARREELLAEQGYQAAHATADTQVGVGKQLGAKFMITGSLTEMRKESPKQ
                     VRISKQEWNYYKLTMEVTDLETGLIAWTTEKEFARQASKPLIGW"
     gene            1416..2639
                     /locus_tag="PKK36_07995"
     CDS             1416..2639
                     /locus_tag="PKK36_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94534.1"
                     /translation="MRLLRKAGVLVLLCAGLAGCATRSLDAARYHFYRGMLDSASERM
                     VEAKPSEKDLALYLMERGTVEQARGNYETSSSDFIRAADELERLETYSLSKGTASMVI
                     NDQVQSFIGTPYERSLLHTFTALNHLSRGDWDNAAVEARRIIQRLDPGKTEGYPLDAY
                     SRYMAGFCLEMIDDPSNAALQYRLANNALPRIEINENTGRIRPLDDDEKKTRVEGAPE
                     PNPWPEEKWENELICFILLDRGPGAQVGWGPAWTSGSAPYAEIHADGKLLGRSYNFAD
                     THDLAFKTAEKQALGEALKTGTRVVLKEAAAQAVASATDNEAAGDLVRLILIGLLERP
                     DVRRWETLPRWLQVARVPCPPDLKEYDVIVRTPGGLQQCYHITQPIARRRGKYFSFFR
                     DLPLPSTLPQASGLR"
     gene            2707..3066
                     /locus_tag="PKK36_08000"
     CDS             2707..3066
                     /locus_tag="PKK36_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94535.1"
                     /translation="MARVLIVDDETDVLETLSVLLRSEGHEVVAVSEGMEAMKKVRSM
                     EHFDLLVSDIRMAPIDGLELLEYARKDRPALAIVVISAYLDDEVIRRAEELGCSQFIR
                     KPFKLEDVIFAIHKALR"
     gene            3108..3467
                     /locus_tag="PKK36_08005"
     CDS             3108..3467
                     /locus_tag="PKK36_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94536.1"
                     /translation="MARVLIVDDEETVLETLRILVECDGHEVIGVTDGSKAIDIIRDD
                     PMLDLVLTDLRMSPVSGMDVMREAKKIKPDLRIIVISAFCDTQTIGEAQNLGCTTYLK
                     KPFNISQVQEAIKSALS"
     gene            3563..4576
                     /gene="pheS"
                     /locus_tag="PKK36_08010"
     CDS             3563..4576
                     /gene="pheS"
                     /locus_tag="PKK36_08010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNR94537.1"
                     /translation="MNAEDIAGIREEALREAAEITDIAALEAFRIKYAGRKGVLQDLM
                     QNLKTADPADRPVLGKVINEVKRDLLALLDERKKAFESAAQAQVAAFDITLPGQWPRP
                     GSRHPISQIIERVVDIFHSLGFTVAEGPDIETVYHNFDALNTPPHHPSRDPQDTFYLD
                     NGDLLRCHTSPVQVRYMEAHEPPVRIVVPGRCYRRDTPDATHSANFHQIEGLYVDRKV
                     SLADLKGDLSHFARELMGPDVKVRFRPHFFPFTEPSVEVDFTCHVCGGKGCRVCKQSG
                     WIEIAGAGMVDPNVFKAVGYDRHQVVGYAFGMGIERIAMILYGIHDIRHLYENDVRFL
                     QQF"
     gene            4582..5607
                     /locus_tag="PKK36_08015"
     CDS             4582..5607
                     /locus_tag="PKK36_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR94538.1"
                     /translation="MSIDRFKTAVSVLFRHPPAWKYDKNRDHAFAEIDRFLEEYVRPG
                     RPCDLKKRPNEVWECVESHLVRAVGEIEKTITLRAAPQGVTVWQMYNSGVIVQLAGRT
                     VALDLLPIIRDFDWVEPPGFTRRIAGMVDMLLVTHRHKDHCDAALVRSCLRSGKPVIM
                     PRPLAETFGSDPLLIPVDGTLETELQNLKITARTGCHVWKETVAELPVAYYEITAPDG
                     FSFIFAGDTDYTKEFEKTPGRDIHLLFIPWRNPNAQYEQGHPLCRGTTLDAVNIALRR
                     IRPRALIYEHYGELKHVYDGFAPSYGMAAWLKKSLKVPSERMFWGEHITMRGLQRISS
                     RSARPRS"
     gene            complement(5576..5971)
                     /locus_tag="PKK36_08020"
     CDS             complement(5576..5971)
                     /locus_tag="PKK36_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94539.1"
                     /translation="MSRVQIILLLAAGIMALESLWGLINPREIKETIGRLLPGMPPRQ
                     PGMGVFFLSLALLLWLAVPRTADLSSWLLLVVVLFTATMGIACLKKNAFRVLAELLIV
                     NRSPSAIRLIYAAEMALAAFMIWAALNGC"
     gene            complement(5968..6543)
                     /locus_tag="PKK36_08025"
     CDS             complement(5968..6543)
                     /locus_tag="PKK36_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94540.1"
                     /translation="MKTAIQMLGGVLLLLSSAVSARESGALNDQPFVPLVRIRLLNSE
                     MIGDEKTEPPGIARQAFKYMGQQAAWHVTPAPGRIGWAAFSFTRPRDMSGLKSDSVLT
                     FALAPADMDDRIGIVIEDRRGNASDVKALSEYRHSELNNRGVYYVPVAGAMLDWTGIS
                     GVRFVVLKPFDERITVAIQNLSIEPRDWGRP"
     gene            complement(6638..7834)
                     /gene="ygeW"
                     /locus_tag="PKK36_08030"
     CDS             complement(6638..7834)
                     /gene="ygeW"
                     /locus_tag="PKK36_08030"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012275764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNR94541.1"
                     /translation="MSKKIKSLIDQLSSLKTGLHEDDFLLTWEQSDADLRAVLLAAEI
                     VEELYREGIQTPAFRNGLAVSIFRDQSTRTRFSFASAASLMGLTLMDMDEKKSQVAHG
                     ETIRETANMISFLTEVIGIRDDMFIGEGHEYMKEVSESVKEGLREGVLPQKPAILNLQ
                     CDRDHPTQSMADLMHLQKTFGSLENLRGKKIAMTWAYSPSYGKPLSVPQGIIGLMSRF
                     GMNVSLAYPKGYDLMPDIVKKAGEFAKASGGKFEVVNSMEDAFADADIVYPKSWAPFS
                     IMEERTKLLRSGESAKLGDLEKQCLANNLQHKDWECTEELMKKTKDGKALYMHCLPAD
                     ITGVSCKQGEVAASVFERYRLRTYKEAEHKPYVMAGMILNNRFKDPAGLLKKFLDDAI
                     PRRGGK"
     gene            complement(7869..9353)
                     /locus_tag="PKK36_08035"
     CDS             complement(7869..9353)
                     /locus_tag="PKK36_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR94542.1"
                     /translation="MKIIESINKEVLARAIQRAKERNIILPTFAQQKDPSKIPAKIVK
                     KLKDVGLWDINPINLFRITWKNEARPSGGGFGEGNWIEFPSELTGVDAKIVGLVGKWF
                     PTGAHKVGAAYGCLVPRLVSGNFDPTTQKAVWPSTGNYCRGGAYDCALLACPAVAILP
                     EGMSRERFDWLHEIGTDEVIPTPGTESNVKEIYDKCWELRRERGDKIVIFNQFDEFGN
                     AIWHYNMTGSVIEEIYNNHYKTDRSRLAAYVSATGSAGTIAAGDYLREYHPGIRVVAT
                     EALQCPTLYQFGFGAHRIEGIGDKHVPWIHNVRNTNMIAAVDDEKTMQLLRLFNEPAG
                     KEFLKKQGLKEDFINQLSLLGISSICNFTAAIKAAKYYEMDSRDVIFFPATDSMELYG
                     SRQTEMTEQHGKYDAVTASNHFGRYLEGIRTDNLRELTYFDQKALHNFKYFTWVEQQG
                     KDSEELRALWDEDFWKELFAPEQVEEWDRLINEFNEGTGLLKAL"
     gene            complement(9449..10216)
                     /locus_tag="PKK36_08040"
     CDS             complement(9449..10216)
                     /locus_tag="PKK36_08040"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNR94543.1"
                     /translation="MRKTAWFLIALCLTSARGEDLSAREILARVREALPSAPMDVQAR
                     YQSKSRRGVIESEGRVDIHTEWGGSPPSARYVLRDLFGEEQQRFEIAWDMNGTASFAL
                     STGSPPAAVGQPDPTAPVPGMDFSWADLSFSFLWWKDARLAGMEKKKGRLCRVVDVMA
                     PDPENGEYAGMRLWIDPEIGLLLQAEGYNLANERIRRMEIKSFKKIEGLWTIQDIDVE
                     SFPSRHKTLLRVRNVEEAAPVLESEPYSDHIGVPAEP"
     gene            complement(10213..>10411)
                     /locus_tag="PKK36_08045"
     CDS             complement(10213..>10411)
                     /locus_tag="PKK36_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94544.1"
                     /translation="VGGIKEKVLAAAAAGIRTVILPKRNLKDLHEVPAEIRRKLKFRG
                     VENMDQVVRAALNVSSRRKKS"
BASE COUNT         2188 a         3121 c         2954 g         2148 t
ORIGIN      
        1 caggcttcgc cccggtcaat tcccgggaga gaatctacgt cgctgaatat gtatttcact
       61 cgccaaggtt ttcttccccg gcagccggct gccggtctac ggcgcaaata tatgcttcag
      121 tatcggatta ttaagcagtt ttgcggcttt tgaacggtat ttttccgctt cgaccagttt
      181 ccttctcaaa aattccttgt tttcgccgcc agccttgtcg tcattaagca gttcgatgct
      241 gcgctggatc tgaatttcag cagtatccag cttcttgttg acggcggaaa gcaggccgtt
      301 gttgatgatg gtactgagat cagactgggc ctgatagtag tctttccggt cacccttgac
      361 ccacacccgc ttaaccgcgc cccagctctc gagctggcgg caggttatac tgacactggc
      421 cttgctgacg cccagcgacg cggccatttc atccagactg cgggggtcgg ggcttaaata
      481 caggaggatg taaatctgtc cgaacagacg actcacgcca aacgactgcg ccgtccttcc
      541 tcctgcatcc accatttctg caacagaccg atgcatacct gctccacctt gtttgcgttt
      601 agaatattct aaacgatttc aatagtcaat tcgccgccaa taactttttt ggcaataatc
      661 cgaccgccat cacgttatgc ggactgatga tcgggcttgc gaacccggga aagttactgt
      721 agttatgtgc cttgggatga cggatgatga ctaaaacctt gaggagggtg ctatgaagtg
      781 gtttgtttct ttgtttgctg cggttttgac ggtttcgctg atgtccgggt gcgcgatgtt
      841 ccgcgcgtcc acatcggatg tggatttgag cgacacgtcc aagcatttcg acgcggattt
      901 tgacgcgacc gacatgcggg gcatcaccga aagcgtggcc gcggaactgc tggccggccc
      961 cttcctgcag ggtcaggcag cgcctccgat cactatgatc gcaggcatcc aaaaccgcac
     1021 cagcaactat gtggacacca agaacctgac cgaccgcatg cgcacgatcc ttctgaagag
     1081 cggcaaaatg cagtttgtca acgaggcccg ccgcgaagaa ctgctcgccg agcagggcta
     1141 tcaggccgcg cacgcgaccg cagacaccca ggtgggcgtc gggaaacagc ttggagccaa
     1201 attcatgatc accggatccc tgaccgagat gaggaaggaa tccccgaagc aggtccgcat
     1261 ttccaagcag gaatggaatt actacaagct gacgatggaa gtcaccgatc tggaaaccgg
     1321 cctgattgcg tggacgaccg agaaggagtt cgcgcgccag gccagcaagc cgctcatcgg
     1381 ctggtaaatt catggccccc gggcggcaat tcactttgcg gcttctgcgc aaagcaggcg
     1441 ttctggtcct gctttgcgcg gggctcgcgg ggtgcgccac acgctcgctt gatgccgccc
     1501 gctatcattt ctaccgcggc atgctggatt ccgccagcga gcgcatggtc gaggccaagc
     1561 ccagcgagaa ggatctggcc ctctacttga tggaacgcgg cacggtggag caggcccgcg
     1621 gaaactatga aaccagttcc tctgatttca tccgggccgc cgacgagctg gagcgcctcg
     1681 aaacctacag cctgtccaag ggcaccgcca gcatggtgat caatgatcag gtgcagagct
     1741 tcatcggaac cccgtacgaa cgaagcctgc ttcacacgtt cacggccctg aaccacctct
     1801 cgcggggtga ctgggacaat gccgccgtcg aggcgcggcg gatcattcag cgtctggatc
     1861 cgggtaaaac cgagggctat ccgcttgatg cgtattcgcg ctatatggcg ggcttctgtc
     1921 ttgaaatgat agacgatcct tccaacgccg cgctgcagta ccgcctggcc aacaacgccc
     1981 ttccccgcat cgagatcaat gagaacacgg gccgcatccg gccgctggat gatgacgaga
     2041 agaagaccag ggtggaaggc gcgccagagc ccaatccctg gccggaggaa aaatgggaaa
     2101 atgaactgat ctgtttcatc cttcttgatc gggggccggg agcccaggtg ggatggggcc
     2161 ccgcatggac ctccggcagc gcgccttatg cggagattca tgccgacggg aaactgctgg
     2221 gccggagcta caactttgcc gacacccatg atctggcctt caagacggcc gaaaagcagg
     2281 ctctgggcga agccctgaaa accggcaccc gcgtggtgct gaaggaggcc gcggctcagg
     2341 cggtggcgtc ggcaacagat aatgaagcgg ctggagacct ggtgcggctg attctgatcg
     2401 ggctgctgga acggccggat gtacggcggt gggaaacgct cccgcgctgg ctgcaggtcg
     2461 cgcgggttcc ctgccctccc gatctgaagg aatatgacgt catcgtccga actccgggcg
     2521 gactgcagca gtgctatcac atcacgcagc ccatagcgcg acggcgcgga aagtattttt
     2581 cttttttccg cgacctgccg ctgccctcca cgctcccgca ggcctccggc ctccgctgat
     2641 ccaccccgcg cacacggctt attttccctt tgcaaaacgg gcggaaccgg gtagtctatc
     2701 catagtatgg caagagtact catagtggac gacgaaacag atgttcttga gacactgagc
     2761 gtgcttctgc gcagcgaagg ccacgaggtg gtggccgttt ccgaaggcat ggaagccatg
     2821 aagaaggtcc gctccatgga gcacttcgat cttctggttt cagacatccg gatggcgccc
     2881 atagacgggc tggagctgct ggaatatgcg aggaaagacc ggccggcgct ggccatcgtc
     2941 gtcatctccg catacctgga cgacgaggtg atccgccggg ctgaggaact cggctgctca
     3001 cagttcatcc gaaagccgtt caagctggag gatgtgatct ttgccataca caaggcgctg
     3061 cgctgaaccg cgccgcggaa tgaggctgat ctgcagggat tgaggatatg gcacgcgtac
     3121 tgatagttga cgatgaagaa acggtcctgg aaacccttcg cattcttgtc gaatgcgacg
     3181 ggcatgaggt cattggcgtg accgacggat ccaaagccat tgacatcatt cgcgacgatc
     3241 cgatgctgga cctggtgctg accgacctgc gcatgagccc cgtcagcggg atggatgtca
     3301 tgcgggaggc aaaaaaaatc aagccggatc tccgcatcat cgtcatctcc gcattctgcg
     3361 acacccagac catcggcgaa gcccagaacc tgggctgcac aacctatctg aagaagccct
     3421 tcaacatctc ccaggtccag gaagccatca aatccgccct gagctgaccg gcagaaaagt
     3481 tccagccatt ggaacttttg cgggaaaaac ttccaatcat tggaactttt ttataggctc
     3541 tttccaatga ttggaacttt ttatgaacgc tgaagacatt gccgggataa gggaagaagc
     3601 actgcgggag gccgcggaga tcacggacat cgccgcgctg gaggctttcc gcatcaaata
     3661 cgccgggcgc aagggggttt tgcaggacct gatgcagaac ctcaaaacag cggatcctgc
     3721 ggaccggcct gtgctcggca aggtcatcaa tgaggtgaaa cgcgatctcc tcgcgcttct
     3781 ggatgagcgc aaaaaagcat tcgagagcgc cgcgcaggcg caggtggcgg cctttgacat
     3841 cacactgccc ggccagtggc cgcggcccgg ctcgcggcat cccatcagcc agatcattga
     3901 gcgcgtggtg gatattttcc attcactcgg cttcacggtg gccgaagggc cggacatcga
     3961 aacggtctat cacaacttcg acgcgctcaa caccccgccg caccatccgt cgcgcgatcc
     4021 gcaggataca ttctatctcg ataacggaga tctcctccgc tgtcacacat cgccggtgca
     4081 ggtgcgctac atggaagccc acgagccgcc cgtccgcatc gtggtgcccg gccgctgcta
     4141 ccggcgcgac acgccggacg cgactcactc cgcgaacttt caccagatcg aaggcctgta
     4201 tgtggaccgg aaggtttcac tggccgacct gaagggcgac ctttcccact tcgcgcgcga
     4261 actgatgggc ccggatgtga aggtccgttt ccgcccgcat ttcttcccgt tcaccgaacc
     4321 cagtgtggag gtggacttca cctgtcatgt ctgcggcgga aaaggatgcc gcgtctgcaa
     4381 gcagagcggc tggatcgaaa tcgccggcgc gggcatggtg gatccgaatg tgttcaaggc
     4441 ggtcggctat gaccggcacc aggttgtcgg ctatgccttt ggcatgggca tcgagcgcat
     4501 cgcgatgata ctttacggca tccatgacat ccgccacctg tacgaaaatg acgttcgctt
     4561 cctgcagcag ttctgaaatc catgagcatt gaccgattca aaacagccgt cagtgtgctg
     4621 tttcgtcatc cgccggcatg gaaatacgac aaaaaccgcg atcatgcctt tgcggagatt
     4681 gaccggtttc tcgaggagta cgtccggccc gggcggccct gcgacctgaa aaaacgaccc
     4741 aacgaggtat gggagtgcgt ggagtcccat ctggtgcggg cggttggcga gatcgagaaa
     4801 accatcacgc tgcgcgcggc gccgcagggg gtgacggtct ggcagatgta caactcgggc
     4861 gtgattgttc aactcgccgg aagaaccgtc gcgctggatc ttctgccgat tatacgcgac
     4921 ttcgactggg tcgaaccgcc cggattcacc cggcgcattg ccggcatggt ggacatgctg
     4981 ctcgttacgc accggcataa agaccactgc gacgccgcgc tggtgcgcag ctgcctgcgc
     5041 agcgggaagc ccgtcataat gcccagaccg ctggccgaaa cattcgggag cgacccgctc
     5101 ctcatcccgg tggacggcac gctcgaaacc gaattgcaga acctgaaaat cacggcccga
     5161 acgggctgcc atgtctggaa ggaaaccgtg gcggagctgc cggtggccta ttacgaaatc
     5221 acggcccccg acggattcag tttcattttt gcaggtgaca ccgattacac aaaagagttt
     5281 gaaaagaccc cgggcaggga catccacctg ctcttcattc catggcggaa tcccaacgcg
     5341 cagtatgaac aggggcatcc gctttgcagg ggcaccacgt tggacgcagt aaacatcgcg
     5401 ctcaggcgga tccggccgcg cgcactgatc tacgagcact acggagagct gaagcacgtc
     5461 tacgacggct ttgccccctc ctacggcatg gccgcatggc tgaagaagtc gctcaaagtc
     5521 ccatcggagc ggatgttctg gggtgagcac atcactatgc gcggcctgca gagaatcagc
     5581 agccgttcag cgcggcccag atcatgaacg cggccagcgc catttctgcc gcatagatca
     5641 ggcgaatggc ggagggcgac cggttcacaa tcagcagttc cgccaggacc ctgaatgcat
     5701 ttttcttcag gcaggcgata cccatggttg ccgtaaacag caccaccact agcagaagcc
     5761 atgaagacag gtccgcggtg cggggaaccg ccagccagag cagcagcgcc agcgaaagga
     5821 agaatactcc catgcccggc tgccgcgggg gcatgccggg aagcagccgg ccgatcgtct
     5881 ctttgatttc gcgcggattg atcagccccc acagcgattc aagcgccatg attcccgccg
     5941 ccagcagcag aatgatctgc acccggctca cggacgcccc cagtcgcggg gctcaatcga
     6001 aagattctga atcgctacgg tgatgcgctc atcaaacggc ttgaggacaa caaaccgcac
     6061 cccgctgatt ccggtccagt ccagcatcgc gccggcgacg ggaacataat aaacgccccg
     6121 gttgttcagc tcggaatgac gatattctga caaggccttc acatcggagg cattcccccg
     6181 ccggtcttca atcacgatgc cgatccggtc atccatgtct gcgggggcca gcgcaaaggt
     6241 cagcaccgaa tcagatttaa ggccgctcat gtcccgggga cgggtgaaac tgaaagccgc
     6301 ccatccgatt ctgcccggag ccggcgtcac atgccacgcg gcctgctgcc ccatgtattt
     6361 gaaggcctga cgggcaatac ccggcggctc cgttttttca tctccgatca tttcggaatt
     6421 cagcaggcgg attctcacca gcgggacaaa gggctgatca ttcaatgccc cggattcgcg
     6481 cgcggaaacg gcggacgaca acagcaacag cacccccccg agcatctgta tagcggtttt
     6541 cactaaagcc atgctagcac aaagattgga aaacgcaaaa ccgcaaaaaa ggggcgggcg
     6601 tccgaggacg cccgcccgtt gccgcacacg ccggggatta ttttccgccg cggcgcggaa
     6661 ttgcgtcgtc gaggaatttc ttcaaaagtc ccgccggatc tttaaagcgg ttgttcagaa
     6721 tcattcccgc catgacatag ggcttgtgtt ctgcttcttt gtaggtgcgg agccggtagc
     6781 gctcaaatac ggaggccgcc acttcaccct gcttgcagct caccccggtg atgtccgccg
     6841 ggaggcagtg catataaagc gctttgccgt ctttcgtctt cttcatcagc tcttccgtgc
     6901 actcccagtc cttatgctgg aggttgttgg caaggcactg cttctcgaga tcgccaagtt
     6961 tggcagattc gcccgagcgc agcagcttcg tgcgttcctc catgatgctg aagggcgccc
     7021 agctcttcgg atagacgatg tctgcatccg cgaacgcgtc ttccatgctg ttgaccacct
     7081 cgaacttccc tccggaagcc ttggcaaact cgccggcctt tttcacgatg tccggcatca
     7141 ggtcatagcc cttcggatac gccagcgaaa cattcatgcc gaagcggctc atcagtccga
     7201 taatgccctg cggcaccgac agcggtttcc cgtaggaggg gctgtaggcc caggtcatgg
     7261 cgattttctt ccctcgcaga ttctccagcg acccgaaggt cttctgcagg tgcatcaggt
     7321 cggccatgga ctgcgtcgga tggtcgcggt cgcactgcag attcaggatg gccggcttct
     7381 ggggaagcac gccctcgcgc aggccttcct tcacggattc ggaaacttcc ttcatgtact
     7441 cgtgaccttc accgatgaac atgtcgtcgc gaatgccgat cacttccgtc aggaacgaaa
     7501 tcatgttggc cgtttcacgg atggtttctc cgtgggccac ctgtgacttc ttctcgtcca
     7561 tatccatcag cgtcaggccc atcaggctcg cggcgctcgc gaacgagaag cgcgtgcggg
     7621 tggactggtc acggaagatg gacacggcga ggccgtttct gaacgccggg gtctgaatgc
     7681 cctcgcggta cagctcttcc acaatttcgg cggccagcag tacggcccgg aggtccgcat
     7741 ccgactgttc ccacgtcagc aggaaatcgt cctcatgcaa acccgtcttg agactgctca
     7801 gctggtcaat cagcgatttg atcttcttgc tcattcaata tctccttaca attgcataat
     7861 tccagggatt agagtgcctt gagcagaccg gttccctcgt taaattcgtt aatcagcctg
     7921 tcccactcct ccacctgctc gggggcaaac agctctttcc aaaaatcctc gtcccagagg
     7981 gcgcgaagct cttccgagtc cttgccctgc tgctcaaccc atgtgaaata cttgaagttg
     8041 tgcaaagctt tctggtcaaa gtaggtcagc tcgcgcaggt tgtcagtgcg gatgccttcg
     8101 agatagcgcc cgaaatgatt gctggccgtc acggcgtcat atttcccgtg ctgctcggtc
     8161 atttcggttt ggcgggaacc gtacagctcc atggaatccg tcgccgggaa gaagatgaca
     8221 tcgcgagaat ccatctcata atatttcgcg gccttgatgg cggccgtgaa attgcagatg
     8281 gaggatatgc ccagcagact gagctgattg atgaaatcct ctttcagccc ctgcttcttc
     8341 aagaattcct tgcccgccgg ctcattgaag agacgcagca gctgcatggt tttctcatcg
     8401 tccacagcgg caatcatgtt tgtgttgcga acgttgtgga tccacggcac atgcttgtcg
     8461 ccaatcccct cgatccggtg cgcgccgaaa ccgaactggt agagcgtcgg gcactggagc
     8521 gcctcggtcg ccaccacgcg gatgcccggg tggtattcgc ggagataatc gcctgcggcg
     8581 atcgtcccgg ctgaacctgt cgcgctgaca taagcggcca ggcggcttct gtcggtcttg
     8641 tagtgattgt tgtagatttc ctcaatcacg ctgccggtca tgttgtagtg ccagatcgca
     8701 ttgccgaact catcgaactg gttgaatatc acgattttgt cgccgcgttc gcgccggagc
     8761 tcccagcact tgtcgtaaat ctctttcaca ttggactcgg ttcccggggt tgggatgact
     8821 tcgtcggttc cgatctcatg cagccagtca aagcgctcgc ggctcatgcc ctcgggcaga
     8881 atggcgacag ccgggcaggc cagcagcgcg cagtcatagg cgccgccgcg gcagtagttc
     8941 ccggtggacg gccagacagc cttctgcgtg gtcgggtcga aatttccgga tacaagacgg
     9001 ggcaccagac acccgtaggc cgcaccgacc ttgtgcgcac cggtcgggaa ccatttgcct
     9061 acaaggccga caatctttgc gtccaccccg gtcagttcag agggaaactc gatccagttg
     9121 ccttcgccaa agccgccgcc actgggcctg gcttcatttt tccaggtaat gcgaaagagg
     9181 ttaatgggat tgatgtccca cagaccgaca tccttcagct tcttaacgat cttggcggga
     9241 atcttggagg ggtctttctg ctgggcaaat gtgggcagaa tgatgttgcg ctctttcgcc
     9301 cgctggatgg cgcgcgcgag aacttcctta ttgatggact ctattatctt catgacagtt
     9361 tttctccttc ggtcattaca aacaagcgga tagaccatat acaaaaaagc cctgatgaca
     9421 accaaatctt ttcaactcaa cctgattttc agggttcagc cggcacgcca atgtgatcgg
     9481 aatagggctc gctctcaagc acaggggcgg cctcctccac attccggacg cgcaaaaggg
     9541 tcttgtgacg cgacggaaag ctttccacat caatatcctg tatggtccac aggccttcga
     9601 tcttcttgaa acttttgatt tccatgcggc ggatccgctc attggcgagg ttatagccct
     9661 cggcctgcag cagcagtcca atttcggggt caatccacag gcgcattccg gcatattccc
     9721 cgttttcagg atcgggggcc atgacatcca caacgcggca gagacggccc ttcttcttct
     9781 ccattcccgc gagacgagca tccttccacc agaggaatga gaagctgagg tcggcccagc
     9841 tgaagtccat tccgggcacc ggcgcggtcg gatccggctg cccgactgct gccggaggac
     9901 ttccggtcga aagagcaaag gaagccgtgc cgttcatatc ccacgcgatt tcaaaccgct
     9961 gctgctcctc cccaaaaaga tcccgaagca cataccgggc ggagggcgga gacccgcccc
    10021 actccgtatg gatgtccaca cggccctcgg attcaatgac cccccggcgc gatttcgact
    10081 gataacgagc ctgcacatcc atcggagcgg agggcagcgc ctcgcgaacg cgagcgagaa
    10141 tctcccgagc cgatagatct tctccccggg cggaagtcag acacagggca atcagaaacc
    10201 aggcggtttt tctcatgact tcttcctgcg ggacgatacg ttcagggcgg cgcgaacaac
    10261 ctggtccatg ttctcgactc ccctgaactt cagcttccgg cggatttccg cgggaacctc
    10321 gtgaagatcc ttcaggttgc gcttcggcag gataacggtt cggatgcccg ccgcggccgc
    10381 ggcaagaacc ttctccttga tcccgccgac g
//
LOCUS       DAOQWR010000091        10250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7001_length_10250_cov_4.862580, whole genome shotgun sequence.
ACCESSION   DAOQWR010000091 DAOQWR010000000
VERSION     DAOQWR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_10250_cov_4.862580"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="PKK36_08050"
     CDS             complement(<1..1612)
                     /locus_tag="PKK36_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR94545.1"
                     /translation="MKIKHSSFYMAVTAVLLLAGTVSLARIVETMTVTAQKDPAFTLS
                     FHDNLFDLSARDAEIQDVLRAIGEAGGIQLKIDQALQGKVTVDLKGASLKNLLNALVR
                     SGSMVFEEKNGAYEMVSATVTSQQQGEEPAPAVPAVPMSDEERENLKARGILSNSGQS
                     IKSLMRKGQSAIILQNAVIDVAEIGRTGKNVEIPEELQAPEDTEYHIVQFDHPATDAD
                     KAALEAAGAALSHYVPYHAYAVHVPAGSMNAVRRIPGVQHVEPYHPYYKMSKDILAYA
                     TGLEAAGDVERLSGGQFNVMLFRGAELPAELKSADVEIVSTSETAGRRLVTVKCPTDK
                     LMELVKADALQWIEPDMPRMPMNDLAARRIRANSLKTLHPTLTGEGVIVNVTDTGIDF
                     LNPGFAMDALLPTTTGLNSRIHYYGYKTGGLTSDGMPGDNNGHGTHVSGSILGNGALS
                     ATANKVPGSGSAPFGPYQFAGMAPGAKVVMLEDFNSFTFAEQTKTSYNKGARISNNSW
                     GNTLFEYGTSSAAWDELVRDADPDAMGNQ"
     gene            complement(1789..2286)
                     /locus_tag="PKK36_08055"
     CDS             complement(1789..2286)
                     /locus_tag="PKK36_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94546.1"
                     /translation="MKSVVIILTAAVLSLPVFAQPPATVSEAIIEAARPAAEDDPAAT
                     TVEAPSDPLAAAEKALAEVQAEREALQAEISPKMTELAKKKHEAEYADPACAKIREEI
                     VELEGRLAELRQQLAAQQLLVPGIAEVNEQRRELIEKIQALSERERVLQNEVRSLRIR
                     APEAE"
     gene            complement(2458..2655)
                     /locus_tag="PKK36_08060"
     CDS             complement(2458..2655)
                     /locus_tag="PKK36_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94547.1"
                     /translation="MLVNGLQVSTADETAKSAKNGSKMPKNAKKSSKMPKNRQKMPIS
                     VKILKKTAEFPEISKIFHLQG"
     gene            2770..6210
                     /locus_tag="PKK36_08065"
     CDS             2770..6210
                     /locus_tag="PKK36_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94548.1"
                     /translation="MLKKLSIFAAIILPLSSSFGAVNLIRNPGFETDVPPGTNDSQAL
                     YWQYGQPDTHGDRFGTAARRTWVPRSGSYHGAIEGSWSKLPGTSGGYWQETNAYQGMT
                     YQASAWFAANTDWSPGYQTLKIEFYNSTTSLLKTVAQTLPSLSTNYAQVTVTGLAPRY
                     TAWVRLVIYVENVSSSGSLWIDDVEMYEIPSRTENFNTWPSLGDGCHEFGGWQVCTGR
                     TTLFGSRTGYAASLPSSNNYVRSPNFENGVGNISFWYRHGSTDTNEESIAAISFEVQK
                     SADGVIWQTIASVSNVNWVSYQPFTKALNESDAHYMRIIHTGGSTNRLLIDDISVANP
                     VPLTRYQDFNAWTNAQANGCHSSNDWIVCTGRITSVMADEGFSATLAPSPTTGFTNYL
                     QTPLMESGYGTISFRYARGTNNTGPAHLNLQSSPDGAAWTTIASVSNIISQSYSDFIQ
                     YFYSTNDAYLRILNIFSTNAGDSTILIDEGFAGANTAPPGWTFSGLGSPPYNSAANSG
                     EEIPALKFDTTGDRVTTPPLAGPTNLSFWLKGISTDVASSFKIEGASGGWTTITNIVD
                     LPTTIAEGFDFSVPVSSVYTQLQFTYTKSSGNAAFDDVLITGLPPGPKPAQQLLLDRI
                     SIGEAEMVRLQDFNSWPTMSGFGDSTHQGWSVQAARVYDPTNAYDGQSARLDKTVDAR
                     AYIQSPYLAGGIGTISFQYARQTAAGSPDPVFVVQISATGASNDWTTLDTLTLNSTAY
                     TEYSRYMYETNKFYLRIYHNSGSGQDAIFDEVQCAEPSPPANMTISGWHDPVQPYTND
                     AVSLWAYVASLNGAKNWSVTSYYRVGTSGVFTALLMDRLSITDYIATNAIAAKTNGTV
                     VQYYMACFFAGPGSDQTSPAYYPAGGPTNPASYGIPRARPGQVWINELSYDTLFPAGN
                     EFIELCGPAGFNISNWKIQLLDGGSNSFSFAQFGYYPVSSPTILSNDAQDHGFWVLGA
                     YNVAGRDMLLTNDLSYFSLPRPTGIRLLNEFGGIEYALSIQGAMPYCEAVPVYEDWFD
                     TEGLSLSGTGTNYDGFSWVNASVTPGTLNTGQSMNTNIPPFSVMDILDWQLGATITIV
                     TTGTNNWSFAPYYSTNLTAGNAWAPVSVFNSDYANGTNTITFPMPAHTSIYYRVRATP
                     "
     gene            complement(6458..6634)
                     /locus_tag="PKK36_08070"
     CDS             complement(6458..6634)
                     /locus_tag="PKK36_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR94549.1"
                     /translation="MKRNDLIRGLEKGGCVFIRHGGKHDWFQNPKTGHCQPVPRHREI
                     NDNLARHIMKQLKD"
     gene            complement(6631..6822)
                     /locus_tag="PKK36_08075"
     CDS             complement(6631..6822)
                     /locus_tag="PKK36_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006529143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR94550.1"
                     /translation="MKSIRYVYWQEGGWWLGYLEDYPDYQTQGQTLEELQQNLKDIFD
                     ELSGGNIPNVRHVGDLQIA"
     gene            complement(7206..9386)
                     /locus_tag="PKK36_08080"
     CDS             complement(7206..9386)
                     /locus_tag="PKK36_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94551.1"
                     /translation="MHRINNKQVVADRGRQGIALIMVLGVLTLITLLAVSFAITMRTE
                     RLAAENYVYAVQARQLLSHALARAIFEADDYLGGSDPMMYPDFSLSGPPLTNAFVSFG
                     TAEAPKDFLYGEVTNFIPRSVWDEAQAAMDHVEWARIQDPGSTNYIGRFAYLLLDCSG
                     LLDANLVGGLDRGPGTNSQEITITSLDDVSPLGTFVTARDTSWIRFETLPELLYVDGL
                     SPPSSNFFVYSRFIDDHLDPKGNPKIRVDQSAAALVDAESEILTALQDCNVNNHNDVY
                     LNLIDYVDTDFIPGNKNGTAAADPLTFCTEPIPMINEISLSNSVRRVSSNAYEHTVYV
                     TVETWYPFPEPPAGIYPTVTLVPPNLSVVSALPDGAMNPDPSAGNTVTVSVDPPSITP
                     AAPNSFQMSTIKYTVLADPYIAGPVYGLQNMTFGNPLMLKFGSGGGGQDVDSAILPTP
                     VLMAVNLTPALGVSETRFGVCNLPHFSCVDPRINWLPSHWTPIAGTPMAINANATGGE
                     GPNIMYVRNEPMQSAAELGFLSVGDVWKTIALCRTNDTPTTDFHPVLDYFTTGPTAIS
                     TNKGLVNVNTRNADVLAAAFASLPVEEYPGDPSAPPLPWADAKVAAEKLIEYLWDNPS
                     SHQISNDLSRIGAPWEIYNKEIDEGPAALLTDARRESLIRNTHRLLGVRQNIFTIILA
                     AQATDNSDTVFGEQRAVAVVWRDPVPDADGRHPCFVRFFKLLAE"
     gene            complement(9370..10179)
                     /locus_tag="PKK36_08085"
     CDS             complement(9370..10179)
                     /locus_tag="PKK36_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR94552.1"
                     /translation="MRRRPHNAAFTLVELLVAMAVLAVIIMMMARIFSESTRAFDLGS
                     KIADQNLKGRMVLDFMAREISQAVADEVMGFRLDSKDIELYADSARPEDGDALYFVTL
                     SENALIDEDSDVHRNAREVVYYVTNMVDSSSGISKPLEGRYRLMRAMRGKQDSIACYQ
                     TNAWWIANGKSGASVLAENVAGFQVWCYTEPGAPAISDYDSTGSKPEQINQLPMWIEI
                     SLMIFDEKDAEHMANLDAFNAPDIPEEAIKRARRYVTRINFVNRNGYASDK"
BASE COUNT         2252 a         3059 c         2707 g         2232 t
ORIGIN      
        1 cctgattgcc catggcgtcg gggtcggcat cgcgcaccag ttcatcccat gccgcgctgc
       61 tggttccata ttcaaacagc gtgttgcccc agctgttgtt ggaaatccgg gcgcccttgt
      121 tgtatgaggt cttggtctgc tcggcgaagg tgaagctgtt gaagtcctcc agcatgacca
      181 cttttgcccc cggagccatg cccgcgaact gatagggtcc gaagggcgcg ctgcccgaac
      241 cggggacctt gtttgccgtg gccgaaaggg ccccgttgcc caggatggaa ccggacacat
      301 gcgtgccgtg gccgttgttg tctccgggca tgccgtctga agtcaatccg cctgtcttat
      361 agccgtagta gtgaatgcgg ctgttcagcc ccgtggtcgt cggaagcagg gcatccatcg
      421 caaaacccgg attcagaaaa tctatgccgg tgtccgtaac gttgacgatc acgccctcgc
      481 ccgtcagggt cggatggagc gttttcaggg agttggcgcg gatgcggcgc gcggccagat
      541 cattcatggg catgcggggc atgtcgggtt caatccactg cagcgcgtct gccttcacca
      601 gctccatcag cttgtcggtc gggcatttga cggtcacaag ccggcggccc gccgtctcgc
      661 tcgtgctcac aatctccacg tccgcgctct tgagttccgc aggaagttcc gcgccgcgga
      721 agagcatcac gttgaactgt ccgccggaga ggcgctcgac atccccggcc gcttcgagtc
      781 ccgtggcata agcgaggatg tctttgctca tcttgtagta cggatgatat ggctcgacat
      841 gctgcactcc gggaatccgg cggacggcat tcatggagcc ggccgggaca tgcaccgcat
      901 aggcatgata aggcacatag tggctcaacg ccgcgccggc ggcctccagc gcggccttgt
      961 ccgcatccgt ggcgggatga tcaaactgca caatatgata ctcggtatct tcaggagcct
     1021 gcagctcttc gggaatttcc acattctttc cggtccggcc gatttcggcc acatcgatca
     1081 cggcattctg aagaatgatg gccgactgcc ccttgcgcat cagggacttg atggactggc
     1141 cggagttgct gagaatgccg cgggccttca ggttctcccg ctcctcatcg ctcatgggaa
     1201 cggccggaac ggccggcgca ggctcttcgc cctgctgctg cgaggtgacc gtggccgaga
     1261 ccatttcgta agctccgttt ttctcttcga aaaccatgga accgctgcgc accagcgcat
     1321 tcagaagatt tttcagactg gcccccttga gatcaaccgt cacctttccc tgaagagcct
     1381 ggtcaatttt cagctggatc ccgcccgcct cgccaatggc gcgcaggaca tcctgaatct
     1441 ccgcgtcgcg ggcggaaagg tcaaagaggt tgtcatggaa cgacagcgtg aatgcgggat
     1501 ccttctgcgc cgtgaccgtc atggtttcca ctatccttgc cagtgaaact gtgccggcca
     1561 gaagaagaac ggccgttacg gccatgtaaa aggaactgtg cttaattttc atcacgactc
     1621 acctcttgac tttcaggatg tcaccgcaaa aacagcgcgc gccgcaggag gaaccttttc
     1681 ggattttcct tcggaccgcg atctcatatg tctaaaaaaa tacaacaaag gacgttacaa
     1741 ccccggcctt tcttcgtcaa tgatttaacc ccttcggaga gagttatttc attcggcttc
     1801 cggagcgcgg attctcagcg agcgcacctc attctgcaaa acgcgctccc gctcgctcag
     1861 ggcctggatt ttctcgatca gttcccggcg ctgttcattc acctcggcga tgccgggcac
     1921 cagcagctgc tgggcggcca gctgctgacg cagctcggcc agacgtccct ccagctcaac
     1981 gatttcctcg cggatttttg cacaggccgg atcggcatac tccgcctcgt gcttcttctt
     2041 ggccagctcg gtcattttgg ggctgatttc agcctgcagc gcctcacgtt cggcctgaac
     2101 ctccgcaagg gctttctcgg ccgcggccag aggatccgaa ggcgcttcca cggtcgtcgc
     2161 cgcgggatca tcttctgcgg cgggccgcgc ggcctcaata atcgcttcag aaactgtggc
     2221 gggcggctgg gcaaagacgg gaagtgacaa aacagcggca gtcagaataa tgacaacgga
     2281 cttcatctgc agttcctttc tgaacgtgaa tccaaattta cacgccaatc tatcccctgt
     2341 tcagaaaaaa gccaagaaga agttgaacat tgcggattgt gaacgaatgg ggggattgga
     2401 atgatggaaa agtggcatga tgggatgatc gaatttgtcc ggaccataaa gaaggtttca
     2461 gccctgcaaa tggaatattt tcgaaatttc cggaaattct gcggtttttt taagaatttt
     2521 aacagaaatt ggcatttttt ggcgattttt gggcattttt gatgattttt tggcattttt
     2581 tggcattttt gacccatttt tagcggattt tgcagtttca tctgcagttg aaacctgaag
     2641 accattcaca agcatcaggg gtcgagtatc aagtttccag tatcaggcat cagcctcttc
     2701 tcccttgcca ccccccaact gtacgggtat attctttaag aaaaccgaat aatcggaggc
     2761 gctcttccca tgctgaaaaa actgagcatt tttgccgcaa tcatactgcc gctgtcttcc
     2821 tctttcggtg ccgtgaacct tattcgcaat cccggattcg agacagatgt ccctccagga
     2881 accaacgaca gccaggccct ttactggcag tatggccagc ccgacactca cggcgaccgg
     2941 ttcggcaccg ccgcgcggcg gacttgggtt ccgcgcagcg gatcctatca cggcgccatc
     3001 gaaggatcct ggagcaagct ccccggaaca tccggaggat actggcagga aaccaacgcc
     3061 tatcagggca tgacctatca ggcttccgcc tggtttgccg caaacactga ctggtcgccg
     3121 ggataccaaa ctttgaaaat cgaattttac aacagtacaa ccagcctcct gaaaaccgtc
     3181 gcccaaaccc tcccctctct cagcaccaac tatgcccagg ttacggtgac cgggctcgct
     3241 ccgcgctaca ccgcatgggt ccggcttgtc atttatgtcg agaacgtcag ctcgtcaggt
     3301 tccctgtgga ttgacgacgt ggagatgtac gaaatcccct cacgcacgga gaatttcaac
     3361 acatggccgt ccctcggcga tgggtgtcat gaattcggcg gctggcaggt ctgcacgggc
     3421 cgcaccacgc tgtttggttc gcgcaccggc tatgccgcca gcctgccctc cagcaacaat
     3481 tatgtccgaa gcccgaattt tgaaaacggc gtgggcaata tctccttctg gtaccgccac
     3541 gggagcacgg acaccaacga ggaaagcatc gccgccatca gcttcgaagt ccagaaatcg
     3601 gccgacggcg tcatctggca aaccattgct tctgtttcca atgtgaactg ggtttcctat
     3661 cagccgttca ccaaggcgct caacgagtct gatgcccact acatgcggat cattcacacc
     3721 ggcggcagca ccaaccgcct gctgatcgat gacatctctg tggccaaccc ggtgcccctc
     3781 acccgctatc aggatttcaa cgcgtggaca aacgcccagg cgaacggatg ccattcgagc
     3841 aacgactgga tcgtgtgcac cggccgcatc acgagcgtca tggcggacga aggcttctcc
     3901 gccaccctcg cgcccagtcc gaccaccggt ttcacgaatt atctgcagac cccgctgatg
     3961 gaaagcggct acgggaccat ttccttccgg tacgcccggg gcacaaacaa caccggcccg
     4021 gctcacctca atctgcagtc ttctccggac ggggccgcat ggaccaccat cgcctcggtc
     4081 tccaacatca tcagccagtc ctattccgac ttcatacagt acttctacag caccaacgat
     4141 gcctatttgc gcattctgaa catcttcagc accaatgcgg gcgattccac catcctgatt
     4201 gacgaaggct tcgccggagc aaacaccgcg ccgcccggat ggactttttc cggactgggg
     4261 agtcctccct ataactccgc cgccaactcc ggcgaagaaa tcccggccct gaaattcgac
     4321 accaccggcg acagagtgac gactcctccc ctggccgggc cgaccaatct gagcttctgg
     4381 ctgaagggga tcagcacgga tgtcgcttcg tccttcaaga tcgagggcgc cagcggcgga
     4441 tggacaacca tcacaaacat cgttgatctt ccgacgacaa tagccgaggg ttttgatttt
     4501 tccgttccgg tatcctcggt ctacacgcag ctgcagttca cctacaccaa aagctccggc
     4561 aatgcggctt ttgacgatgt gctgatcacc gggctcccgc ccggacccaa gcctgcccag
     4621 cagctcctgc tggaccgcat cagcatcggc gaggcagaga tggtccgcct gcaggacttc
     4681 aacagctggc ccaccatgag cggattcgga gacagcactc accagggctg gtctgttcag
     4741 gctgcgcggg tctatgaccc gaccaatgcc tacgacggcc agtcggcacg gctcgataaa
     4801 accgttgatg cccgcgcgta catccagagc ccctatctgg cgggaggcat cgggaccatt
     4861 tccttccagt atgcgcggca gaccgccgcc ggatcgccgg atccggtttt tgtcgtccag
     4921 atttccgcaa cgggggcctc caacgactgg accacgctgg acacgctgac attgaattcc
     4981 acggcatata ccgaatacag ccgctacatg tatgagacca acaagttcta tctccggata
     5041 taccacaatt ccggctccgg acaggatgct atcttcgatg aagtgcagtg cgcggaaccg
     5101 tctccgcccg cgaacatgac catcagcggc tggcatgatc cggtccagcc gtacaccaac
     5161 gacgcggttt cgctgtgggc atacgtcgct tccctgaacg gggcaaagaa ctggtcggtg
     5221 acgtcctact atcgcgtggg aacaagcggc gtgttcaccg ccctgctgat ggaccggctg
     5281 agcatcacgg actatattgc caccaatgcc atcgcggcca agaccaatgg gacggtggtg
     5341 cagtactata tggcctgctt ctttgcgggg cccgggagcg accagaccag tcccgcctat
     5401 tacccggccg gcgggccaac caatccggca tcctacggga tcccccgggc cagacccggg
     5461 caggtgtgga tcaatgaact cagttacgac acgctcttcc cggccggcaa tgagtttatt
     5521 gagctctgcg ggcccgccgg attcaacatc agcaactgga agattcagct tctcgacggc
     5581 ggatcgaaca gcttctcatt cgcgcagttc ggatactatc ccgtcagcag cccgaccatc
     5641 ctctccaacg acgcccagga tcatggattc tgggtgctgg gggcctacaa cgtggccgga
     5701 cgcgacatgc tgctgaccaa tgacctcagc tacttctctc ttccccgccc gacggggatc
     5761 cggctcctta acgaattcgg cggcatcgaa tacgcgctga gcattcaggg ggcgatgccg
     5821 tactgtgagg ctgttccggt ttatgaggac tggttcgaca cagagggatt gagcctctcg
     5881 ggcaccggca caaattatga cggattttca tgggtcaatg catctgtgac tccgggaact
     5941 ctgaataccg gccagagcat gaacaccaac atcccgccgt tctcggtaat ggacatcctt
     6001 gactggcagc ttggcgccac catcaccatc gtgaccaccg gcacaaacaa ctggagcttc
     6061 gcgccgtact attcgaccaa cctcaccgcg ggaaatgcgt gggcgccggt gtcggtgttc
     6121 aacagcgatt acgccaacgg aaccaacacc atcaccttcc cgatgccggc ccatacgtcc
     6181 atttactacc gcgtccgggc gacgccgtaa aagagattga tgctggatac ttgatgcttg
     6241 aaaccggagt tgcaggttta aagttgaagg ttgcaggcag gaatcaggcc ttcatatgag
     6301 aagagcattt tagtctgtag tcatttaggc tgaaggaagc attcagaatc tctgcgtctc
     6361 agcgcccctg cgggaaatac ttcgcatggg aacatgttga agagaaggaa aagagacagg
     6421 gtttacagga tcaacaagat aaaagtctct ggtctttcta atccttcaac tgcttcatga
     6481 tgtgccgcgc aaggttatca ttgatctcgc gatggcgcgg aacgggctgg caatgtccag
     6541 tttttggatt ctgaaaccaa tcgtgcttgc cgccatggcg aataaagacg cacccaccct
     6601 tttccaggcc gcggatgaga tcatttcgct tcatgcaatc tgcaaatctc ccacgtgccg
     6661 gacattgggg atgttcccgc cagagagttc atcaaagata tctttcagat tctgctgaag
     6721 ttcctcaagc gtttggccct gggtctgata atcggggtaa tcttccagat aacccagcca
     6781 ccaaccgccc tcctgccaat agacataacg aatggatttc atgcccgtac cttagcccga
     6841 catgtggata gattcaacca aatattccgg gcaacatccg cctccatctc tgcgccccag
     6901 cgtccctgcg ggaaaatctt cgcctggaaa tatggtgtag tagtcgttct gcgagcgaca
     6961 tcttttcggg aaatgcgcct cacagaggcg ctactacact cataaccccg cacgaagacg
     7021 cagagacaca aagagttaat gtcacgtcct ttgcgacttg gtgtctttgt gcgaaccctt
     7081 ttccggaaaa tgtttcacca tgaagttcat gacgaagaac gaatctgaaa acctgcaact
     7141 tgcaacctga aacctttaac cgttcacgat ctgaagtctc tagtctgtag tctctggtct
     7201 ctagtctatt ctgccagcag cttgaagaac cggacaaagc agggatgcct gccgtctgca
     7261 tccggaaccg gatcgcgcca gaccaccgcc acggcgcgct gttcgccaaa aaccgtgtcg
     7321 ctgttgtccg tggcctgcgc cgcaaggatg atggtgaata tgttctggcg aacacccagc
     7381 agcctgtgcg tgttccggat cagggactca cggcgggcat ccgtaagaag cgccgccgga
     7441 ccctcatcaa tttccttatt gtaaatttcc cacggcgcgc cgatccgcga caggtcattg
     7501 ctgatctgat gcgaagacgg attgtcccaa aggtattcta taagtttttc tgccgcgacc
     7561 tttgcatccg cccagggcag aggcggcgcg gagggatctc ccggatattc ttcaaccggg
     7621 agcgatgcaa acgccgcggc aagcacatcc gcgttgcggg tgttcacatt gaccagcccc
     7681 ttgttggtgg agatggcggt cggccccgtc gtgaaatagt ccagaacagg gtgaaagtcc
     7741 gtggtcggag tgtcgttcgt cctgcacagt gctattgttt tccagacatc gcccacactc
     7801 aaaaacccca gttcagccgc tgattgcatg ggttcgttgc gcacatacat gatgttgggc
     7861 ccttcgcctc cggtcgcatt cgcattgatt gccatgggcg ttccggcaat tggagtccag
     7921 tgggagggca gccagttgat gcgcgggtca acacaggaaa aatgtggcaa attgcatacc
     7981 ccaaacctgg tttcgctcac accaagagcc ggtgtcaaat taacagccat aagcaccggt
     8041 gtcggaagta tcgcggaatc cacgtcctgc ccgccgccgc ccgaaccgaa tttcagcatc
     8101 agagggttgc caaaagtcat gttctgaaga ccgtaaactg gtcctgcaat gtacgggtcc
     8161 gcaagcacgg tgtatttgat cgtggacatc tggaatgaat tcggcgcggc cggtgttatg
     8221 cttggaggat ccacgctgac cgtgaccgta ttcccggccg agggatccgg attcatggcg
     8281 ccatccggaa gcgcactgac cacactcagg ttcggaggaa ccagtgtcac tgtgggataa
     8341 atgccggccg gcggttcagg aaacgggtac cacgtctcaa cagttacata cacggtgtgt
     8401 tcataggcat ttgacgaaac gcggcgcacg gagtttgata aactgatctc attgatcatc
     8461 gggatgggtt cggtgcagaa cgtcaaagga tccgcggccg cagtcccgtt cttgtttccc
     8521 ggaatgaaat ccgtgtccac atagtcaatc aggttcaagt acacatcatt gtggttgttg
     8581 acattgcagt cctggagcgc cgtcaggatt tcgctctccg cgtccaccag cgcggccgcg
     8641 ctctgatcca cgcggatctt cggattcccc ttcggatcca gatggtcatc tatgaaccgg
     8701 gaatacacga agaaattgct cgagggcggc gacaatccat ccacatacag cagttccggc
     8761 aacgtctcga aacgaatcca cgaagtgtct ctcgccgtta caaacgtacc caagggggaa
     8821 acatcatcaa ggcttgttat ggtaatttcc tgagagtttg tgccaggccc ccgatccagg
     8881 cctccgacca gattcgcatc gagcaggccg gagcagtcga gcagcagata ggcaaaacgc
     8941 ccaatgtagt tcgtcgaacc cgggtcctgg atcctggccc actccacatg atccatggcg
     9001 gcctgcgctt catcccacac cgagcgggga ataaaattgg taacttcacc gtacaggaaa
     9061 tccttgggcg cctcggcggt tccaaacgac acaaaggcgt tggtcagggg cgggccggag
     9121 agggagaaat cgggatacat catggggtca gaaccgccca ggtaatcgtc cgcctcaaaa
     9181 atcgcccggg ccagcgcgtg actgagaagc tgtctcgcct gaaccgcata gacataattc
     9241 tccgcggcaa ggcgctcggt gcgcatggtg atcgcaaaag acacggccag cagcgtgatc
     9301 agcgtcagca cgcccagcac catgatcagg gcgatgccct gccggcccct gtccgccacg
     9361 acctgtttgt tatttatccg atgcatatcc gttccggttc acaaaattaa ttcgcgtcac
     9421 ataacggcgg gcccgcttga tcgcttcctc tggaatatcc ggagcattga atgcatccag
     9481 attcgccata tgctcggcat ccttctcatc aaatatcatc agcgatatct ctatccacat
     9541 tgggagttgg ttgatctgtt ccggcttgct cccggtcgaa tcataatcag aaatggcagg
     9601 cgcccccggc tccgtgtagc accagacctg aaatcccgcc acgttttccg ccaaaacact
     9661 tgccccagac ttcccattag ctatccacca agcatttgtc tgataacaag cgatagaatc
     9721 ctgtttacct cgcatcgctc gcatcagccg ataacgtccc tcaagcggct tggatatccc
     9781 gcttgatgaa tcaaccatat tcgtgacgta atagacaact tcgcgcgcat ttcgatggac
     9841 gtccgaatcc tcgtcgatga gtgcattctc tgaaagagtc acgaaatata gagcatctcc
     9901 atcttcaggg cgcgctgaat cagcatacaa ctcgatgtct ttcgaatcca atctaaatcc
     9961 catgacttca tccgcgacag cctgcgaaat ttcgcgggcc atgaaatcca gcaccatccg
    10021 ccccttcagg ttctggtcgg caatcttgga gcccagatca aaagcgcggg tgctctcgga
    10081 gaatatccgg gccatcatca tgatgataac ggccagcacc gccatggcca ccagcagttc
    10141 gaccagcgtg aaagccgcgt tgtgcgggcg ccgcctcatg atttataagc cgtagttgta
    10201 aaaggttgtg acatatatat aggcatcctt ctcgtcggaa ctgccgaact
//
LOCUS       DAOQWR010000092        10229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7013_length_10229_cov_5.219186, whole genome shotgun sequence.
ACCESSION   DAOQWR010000092 DAOQWR010000000
VERSION     DAOQWR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7013_length_10229_cov_5.219186"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKK36_08090"
     CDS             <1..356
                     /locus_tag="PKK36_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNR94553.1"
                     /translation="PLDVELAFEDRPKPSGRDLSARRLGLVFGDPGAQQSPYPMGLPV
                     ERVEEGGPAAQAGLRPGSWISRINRTEIRTLDDAGAALQNVKTGDPVGLVAVKLEEQG
                     AFMVSQRSTLSVQAR"
     gene            440..1213
                     /locus_tag="PKK36_08095"
     CDS             440..1213
                     /locus_tag="PKK36_08095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94554.1"
                     /translation="MSETDKTIHDFDFKLICEYYAGMERQGPGSPDVTLRALSFVDNL
                     TGESRIADLGCGTGGQTMVLAGHAPGRITGIDLFADFIDIFNAASRKLNLQDRVSGMV
                     GSMDKLPFRDEELDLIWSEGAIYNIGFERGLNEWRRFLKKGGFIAVSEASWFTEERPA
                     EIDEFWKDAYPEIDTISNKVGQMQKAGYIPVAAFILPESCWTENFYVPQVPLQQEFLR
                     KHAGNKMAGDLVENQRHEAELYYKYKDFYGYAFYIGKKI"
     gene            1355..2302
                     /locus_tag="PKK36_08100"
     CDS             1355..2302
                     /locus_tag="PKK36_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR94555.1"
                     /translation="MIKVKHLTRRFGRKLAVDDLSFDVARGEVLGFLGPNGAGKTTTM
                     RIITGFLAPTGGEVEVDGLNVMTHSREIRSRIGYLPENVPLYPEMRVDEYLGFRARLK
                     GLSGRRCRARLNQVKAQCGLENEGRRVIGYLSKGYRQRVGLADALIAEPELLILDEPT
                     IGLDPNQIRQVRTLIRDLAEKHTIVLSTHILHEVEMTCRRVLIISGGRLVASDSPSSL
                     RKMMLAGAPLIADIRGPQDTVAAKLSALPAVKQVQVMRAGEWTKYQLAVEPGADLRAD
                     LSELVVRSGWGLRELTTGRPTLEDVFVRLTCDAEGGLPP"
     gene            2299..3042
                     /locus_tag="PKK36_08105"
     CDS             2299..3042
                     /locus_tag="PKK36_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR94556.1"
                     /translation="MRTFLILWKRELAAYFLSPIAYVMMMFFLAVMGYTFWMLLNILA
                     HGVVGATVMNELFGSIFFWITLLILIPLITMRLLAEENRSGTIELLMTAPVTEAQVVL
                     AKYAGALSFYFIMWMPTLAYVYVLRRYDPLMSVDLGPLAAGYLGALLLGAFYLSAGLL
                     MSALTRNQIVAAMSAFAMVTLAFFAGFMPYISRSETVRDAGRNFSAVMHMMDFSRGMV
                     DTRPAVLYLTGAAFLLFTTVKILEMKKWK"
     gene            3033..4544
                     /locus_tag="PKK36_08110"
     CDS             3033..4544
                     /locus_tag="PKK36_08110"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNR94557.1"
                     /translation="MEVNDSPRPGGPRDARGGLRRQRLLRTAARLNAVAAILLALVLA
                     VMANYLSIRHYRRMDWTRTRYYSLSQRTLDLLGSLTNDIRVIVFFQPEHQVYTDVENL
                     LREYEYASSRIRAEYVDPARDLARTEELAQKYQVGEANVVVFDSGARSKYVGLDDMVE
                     FSYPGMEEGRIPEAVAFKGEQAFSSAIQDISQSHSPVVYFLEGHGERSVEDFDQRRGY
                     SSLARMIRRDNITVKTVRFGEMTALPEDTDALVIAGPARRFAQPEIDLIGTHLAHNGR
                     LILMLDKDVRTGLAPLLEEWGIRVGNDVVTDPARTLTGDDLFITEYGEHPITAQMKGL
                     TTIFYQPRSVSPAAGTGGAGDPSDKPRVVSLASCSADGWAETRPGDGLFQFDPLEDRR
                     GPVPVAVAMERGAAPALDVRIRPTRAVVFGDSDFVCNGAMAGGGLDFFMSALNWVLER
                     EELLAIAPKTIVESRLILNRHDLRELFWILVPGLPLAVALAGLLVWLRRRRGA"
     gene            4541..6346
                     /locus_tag="PKK36_08115"
     CDS             4541..6346
                     /locus_tag="PKK36_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNR94558.1"
                     /translation="MNGKSTLVLLMTVVLVGLFIVLFERNTDSTRTRMDHAGRAFYFN
                     PDSVTAIEVSSDGVSFECRREKSGWTMKKPAEEPADGGAVDELLYRLSTVSRGETITP
                     AQLAARNLHPEDFGLDRPRVRIAVTDAGRTSVYLLGASTPLGNALYAAVEGHAEVFTA
                     DGGVLDVLPADADLLRSRRLFHEERSKVRQIAVHREEGFLQVERDKEGRWLLRQPVTG
                     RADRNRIYEWLDALFKYEISDFTASETGDASAYGLTDSQLQITLTFNGDDSGSTLILG
                     RATAENPRLIYARRAAGRAVYAVPAALADLLRVKEADWRERRLLPLAPHEINGIRMGS
                     GSDSIVLSRSAAGRWMVGGLQPVAADPERVGALLNAWASAEIVEFNDAAGTNLAVFGL
                     APEKMRVSFLKNPDRPESAFELLVGSLPPEDGRVWVRAVTEKPLYRIPSEVIERISLD
                     RLFYRNREVLTTDPARIRSITLKGAGREETLQMDSVTGKFAPVRPRQDKPDELAVQKV
                     LVSVSRLVAGRFVAEDPADLNRYGLAPPADELTIGLAGQDGISKTLMLGNESEDGGFY
                     AMIKGQDIIFTLDREIRDAAFPATLFSVPPAGETP"
     gene            6343..8811
                     /locus_tag="PKK36_08120"
     CDS             6343..8811
                     /locus_tag="PKK36_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94559.1"
                     /translation="MTTGRQAGRTLSRPVHALKRCFRCFLLIVLLLLAAGLYMNRVGL
                     PDAVKNRVVRELAARGVRVEVARVRWELLEGIVMDDLLYFAGALDPEPVVRAEEATVN
                     LRPAAWFRGRHGVRGFAIRNARLGTGEGLSGIHASVRLEDEGLQIRDLFADYGGLKIT
                     GEGFIARPQANMNGALMQAWRQIFPPSDKTEKTPALRVLLEDLKAVQFAQGSSAQIML
                     VLHPDDLNANELKIEARGGGLTLRGVNLEGWRMQAALKNGRFTLARLSVASGQQAVRA
                     AGFLDLTDGTAACKLFSDLPPYVLLALAPVSLRQTLDSLEILPGEALEFTADAGPAPV
                     GELLDVVHGSLALKDSRLLGLPVASTSLEFSREHQVLAVTNLQASVGLESRSGPLTGY
                     ARYDLESQTYSGRMNTGFDPGWMCPLVSTGKARVISGLFFRENLPQTSFTFSGQRGDD
                     GAPLQVEGSVRGRNFSFRGTYFTSVESKLVMTNGVLILDPVTARRGSRACRGRIAFDF
                     RNDIVDVDVESTFDPAATLRIMGPTLERLSRPFRFDGYTRISAKGSIDYETLTNMNLE
                     AHVVASNMTYQHVAVESCAFDLLAKGRRFEVRNLQGVLYGGRLEMSRAVMESLADSPL
                     WRYELALSLAGADFGRLMQAYRRVGADHPYEGRLDFMLAVAGETGEQMLLRMRGRGDI
                     HISESRLLRIPLFGGLAKILSALYPNFGIVSKNDLTADFKIGGNSIRTQNARLLGHII
                     SVQGSGRYHFTNNLDFKVRVRMLREGRLAALLDWLTFPVTKLLEFHLSGPLEQPVWRP
                     DNLPKELFNMLKSVIPGIGDEAKR"
     gene            8795..9658
                     /locus_tag="PKK36_08125"
     CDS             8795..9658
                     /locus_tag="PKK36_08125"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNR94560.1"
                     /translation="MKQNADHAWHVLTVELPEEHAEALQEWIRDRYGLEPVLLERAGA
                     RGQWIETYFPDASAAREAAVETAEAFPVRATAVRLLEARDWQNFWRHHFIRREIGRSL
                     LIVPDWEQNGDTGGRAVIRLEPGLSFGTGDNFTTRFCLEMIDELFAKRVPESFLDVGA
                     GSGILSIAAAKLGCPRIMALENDALSVASARENLERNGVTSLVTIEAHDILSGWDGGR
                     YEFICANIISGVLTRAAPVLAQTASRLLVLSGILEWECDGVAEIYQRLGGREVIRDGD
                     GQWCGLVFEFN"
     gene            9735..>10229
                     /locus_tag="PKK36_08130"
     CDS             9735..>10229
                     /locus_tag="PKK36_08130"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HNR94561.1"
                     /translation="MSFDWNILMLGTVIFLARIMDVSLGTLRIISVVQGRTWTAFFLG
                     FFEVGIWITIISAVMHYINEQPILGFFYALGFAAGSVVGIKIERRIASGHIALRLISY
                     EHGEAMVEAIRRAGYGMTVYEGMGKHGPVRELFLVCLRKDRSAILKIAKSIDPDVFST
                     TSPAD"
BASE COUNT         1849 a         3060 c         3248 g         2072 t
ORIGIN      
        1 aaccgctgga tgtggagctt gccttcgagg accggcccaa gccgtcggga cgcgacctgt
       61 ccgcgcgccg gctgggactt gttttcggcg atcccggagc gcagcaaagc ccctatccga
      121 tgggcctccc cgtcgagcgc gttgaagagg gcgggccggc cgcgcaggcg gggctcaggc
      181 cgggcagctg gatctcgcgc atcaaccgca cggaaatccg gacccttgat gatgccggcg
      241 ccgcgcttca gaacgtgaag accggcgacc cggtcggcct ggttgcggtc aagctcgaag
      301 agcagggcgc cttcatggtt tcccagcgtt ccacgctcag cgttcaggcc agatgaacaa
      361 tctcagtgtg ggccgcagcg gaggatgccg tctgtgatca acaagaagaa accttttacg
      421 ggacaagcag ggagatgcca tgagcgagac ggataaaacg attcatgatt tcgatttcaa
      481 gctgatctgc gaatactatg cgggcatgga gcggcagggg cccggcagtc ctgatgtgac
      541 tctcagggct ttgagcttcg tggacaacct caccggcgag tcaaggattg ccgatctggg
      601 ctgcgggacc ggcgggcaga cgatggtgct ggccggtcat gctccgggac ggatcacggg
      661 gattgatctg tttgccgact ttatagacat cttcaatgcc gccagccgta aactgaatct
      721 tcaggacagg gtcagcggta tggttggatc aatggacaaa ctccctttcc gggacgaaga
      781 gctggacctg atctggtctg aaggggccat ttacaatatc ggattcgaac ggggcctgaa
      841 cgaatggcgc aggtttctga aaaaaggcgg tttcattgcc gtttcagagg cctcctggtt
      901 caccgaagag aggcctgccg agatagatga attctggaag gatgcctatc cggaaataga
      961 caccatctca aacaaagtcg ggcagatgca gaaggccggc tacattccgg tcgccgcgtt
     1021 tattctgcct gaaagctgct ggaccgaaaa tttttatgtt ccgcaggttc cgcttcagca
     1081 ggaattcctg aggaaacatg ctggaaataa aatggccggg gatctggtgg aaaatcagcg
     1141 tcatgaagcg gaactctatt acaaatacaa agatttttac ggatatgcct tttatatcgg
     1201 gaagaagatt tagactgcgc gtcgggccgg acagccttcc gggcgccccg gcgctccctg
     1261 gtttggtgct ggccgtttca taaagaagac ttgccggagc aaataagcct gataagcttt
     1321 ccttttcctt aacgagagca gttttgaact gataatgatc aaagtgaaac atttaacccg
     1381 gcggttcggc cgcaagctgg cggtggatga cctttccttt gatgtggccc gcggcgaggt
     1441 gctcggattc ctcggcccga acggcgccgg caaaaccacc accatgcgga tcatcaccgg
     1501 attcctcgcc ccgaccggag gcgaagtcga ggtggacgga ctgaatgtca tgacccattc
     1561 gcgcgaaatc cgcagccgca tcggttacct gccggaaaat gtcccgctct atccagaaat
     1621 gcgcgtggac gaatatctgg gtttccgcgc gcggctcaag gggctttccg gccgccgctg
     1681 ccgggcccgg ctgaaccagg tcaaggcgca gtgcgggctg gaaaacgaag gccgccgcgt
     1741 gatcggatat ctttccaagg gctaccgcca gcgcgtgggc ctggccgatg cgctgatcgc
     1801 ggagccggaa cttctgattc tggacgagcc cacgatcggc cttgatccca accagatccg
     1861 gcaggtgcgc accctgatcc gggaccttgc ggagaagcac accattgtgc tttccacgca
     1921 cattctccac gaagtcgaaa tgacctgccg ccgcgtgctc atcatcagcg gcggcagact
     1981 ggtggcgtcc gattcgcctt ccagcctgcg caaaatgatg ctggccggcg cgccgctgat
     2041 cgccgacatc cgcggcccgc aggacaccgt cgccgcaaag ctcagcgccc tgccggccgt
     2101 gaagcaggtg caggtgatgc gcgcggggga atggacaaaa taccagctcg cggtggagcc
     2161 gggcgcggac ctccgcgccg acctgagcga actggtcgtc cgcagcggct ggggcctgcg
     2221 cgaactgacc accggccggc cgacgcttga ggatgtgttt gtgcgcctga cctgcgacgc
     2281 cgagggaggt cttccgccat gagaaccttc ctgatcctgt ggaaacgaga actggccgcg
     2341 tattttcttt cgcccattgc ctatgtgatg atgatgttct ttctcgcggt gatgggttac
     2401 accttctgga tgctgctgaa tatccttgcg cacggcgtcg tcggcgcgac cgtgatgaac
     2461 gagctcttcg gatccatctt cttctggatc accctgctga tcctgatccc gctgatcacc
     2521 atgcggcttc tggcggagga aaaccgttcc ggcaccatag agctcctcat gaccgccccg
     2581 gtcacggagg cgcaggtggt gctggccaaa tacgccgggg ccctgtcttt ctatttcatc
     2641 atgtggatgc cgacactggc ctatgtctat gtgctgcggc gctacgatcc gctgatgagc
     2701 gtggacctcg gcccgctggc cgccggctat ctcggcgcgc tgctgctggg ggctttctat
     2761 ctcagcgccg gcctgctgat gtccgcgctg acgcgcaacc agatcgtggc ggccatgtcc
     2821 gcgtttgcga tggtgacgct ggcctttttt gcggggttca tgccgtacat ctcgcgctcg
     2881 gaaacggtgc gcgatgccgg acggaacttt tcggccgtga tgcacatgat ggatttctcg
     2941 cgcggcatgg tggacacgcg cccggccgtg ctgtatctga ccggcgccgc atttcttctc
     3001 ttcacaacgg ttaagatcct ggaaatgaaa aaatggaagt gaacgacagt cccagaccgg
     3061 gcggtccgcg ggatgcccgc ggcggcctgc gccggcagcg cctgctccgg acggcggcgc
     3121 ggctcaacgc cgtggccgcc atcctgctgg cgctggttct cgcggtgatg gcgaactatc
     3181 tttcgattcg tcactaccgc cgcatggact ggacgcgcac ccgctattac tccctttccc
     3241 agcgcacgct ggacctgctc ggcagcctca ccaacgacat ccgcgtgatc gtgttcttcc
     3301 agccggagca tcaggtctat accgatgtcg aaaacctgct gcgcgagtat gagtatgcct
     3361 cttcgcgcat ccgcgctgaa tatgtggatc ccgcccgcga tctcgcgcgg accgaggagc
     3421 tcgcgcagaa ataccaggtc ggcgaagcca acgtggtggt gttcgacagc ggcgcgcgca
     3481 gcaagtatgt cgggctggat gacatggtgg aattctccta tccgggcatg gaggagggcc
     3541 gcattcccga agccgtcgcc ttcaagggcg agcaggcctt ttcgtccgct atccaggata
     3601 tctcccagtc ccacagtccg gtcgtgtatt ttctggaagg gcacggcgaa cgttcggtgg
     3661 aggattttga ccagcgccgg ggctattcct cgctggcgcg catgatccgc cgcgacaaca
     3721 tcaccgtgaa aacggtccgg ttcggcgaga tgaccgctct gcccgaggac accgatgcgc
     3781 tggtcattgc ggggcccgca agacgcttcg cccagccgga gattgatctg atcggaaccc
     3841 atctggctca taacggacgg ctgattctga tgctcgacaa ggatgtgcgc accggcctgg
     3901 ccccgcttct tgaggagtgg ggcatccgtg ttggaaatga cgtggtgacc gatcctgcgc
     3961 gcacactgac cggtgatgat ctcttcatta ccgagtacgg cgaacatccc atcaccgcac
     4021 agatgaaagg gcttacgacc attttttatc agccgcgctc cgtgtcgcca gccgccggta
     4081 ccggcggcgc gggcgatccg tccgacaagc cccgcgtggt gagtctggcc tcctgctcgg
     4141 ccgacggctg ggccgaaacc cgccccgggg acggcctgtt tcagtttgat ccgctggagg
     4201 accgccgcgg ccccgtcccc gtcgccgtgg ccatggagcg gggcgccgcg cccgcgctgg
     4261 atgtccgcat ccggcccacc cgtgccgtgg ttttcggcga ttccgatttt gtctgcaacg
     4321 gcgcgatggc gggcggcggc ctggattttt tcatgagcgc gcttaactgg gtgctggagc
     4381 gcgaggagct gctcgcgatt gccccgaaga ccatcgtgga aagccgtctg atccttaatc
     4441 ggcatgattt gcgggagctg ttctggattc ttgtgcccgg cctgcccctg gcggtggcgc
     4501 tggccggcct gctggtctgg cttcggaggc gtcgcggcgc atgaacggca aatccaccct
     4561 tgttctgctg atgacggttg ttctggtcgg actctttatt gttttgtttg aacggaatac
     4621 ggacagcacc cggacccgca tggatcatgc cggccgtgcc ttttatttca atccggattc
     4681 ggtcacggcg atcgaagtga gcagcgatgg cgtcagtttc gaatgccgcc gcgaaaaatc
     4741 gggctggacc atgaagaagc cggccgagga gcccgcagac ggcggcgcgg tggatgaact
     4801 gctctaccgg ctttcgaccg tgagccgcgg agaaaccatc acgcccgcac agcttgccgc
     4861 ccgcaatctg catccggaag atttcggtct ggaccggccg cgcgttcgca tcgctgtgac
     4921 ggatgccgga cgaacgtcag tctatctgct gggcgcatcc acgccgctgg ggaatgcgct
     4981 gtacgcggct gtggaggggc acgcggaggt tttcacggcc gacggcggcg tgctggatgt
     5041 gctgcccgct gatgccgacc tgctgcgttc gcgccgcctg ttccatgaag aacgctccaa
     5101 agtccggcag atcgcggttc atcgcgaaga gggttttctg caggtggagc gtgacaagga
     5161 gggccgctgg cttctccgcc agccggtcac gggccgggcc gaccgcaatc gaatctacga
     5221 gtggcttgat gcgctgttca aatatgagat ctcggatttc acggccagcg agaccggcga
     5281 tgccagcgca tacgggctga ccgactccca gctgcagatt acactcacct tcaacgggga
     5341 cgacagcgga agcaccctga tcctcgggcg cgccacggcc gagaatccga gactgatcta
     5401 tgcccgccgc gccgccggcc gggcggtgta tgctgtgccc gccgcgctgg ccgacctgct
     5461 ccgcgtaaaa gaggcggact ggcgcgaacg gcgacttctg ccccttgcgc ctcatgaaat
     5521 caacggcatt cgcatgggct cgggttccga ttccattgtt ttaagccgca gcgccgccgg
     5581 gcgctggatg gtcggcggac ttcagccggt tgccgccgac cccgaacgcg tcggagccct
     5641 gctgaatgca tgggcttccg ccgagatcgt ggagttcaat gatgcggcgg ggaccaacct
     5701 ggccgtcttc ggcctggctc cggaaaaaat gcgtgtcagt ttcctgaaga atcccgaccg
     5761 gcccgaaagc gccttcgagc ttctggtcgg aagtctgccg ccggaggacg gccgcgtctg
     5821 ggtcagggct gtgacagaga aaccgcttta ccggatcccg tcggaggtca ttgaacgcat
     5881 cagtctggac cgcctttttt accgcaaccg ggaagtgctg acgaccgacc ccgcgcggat
     5941 ccgcagcatc accctcaaag gggcggggcg ggaagaaacg cttcagatgg attctgtcac
     6001 cgggaagttc gctcccgtcc gtccacgcca ggacaagccg gatgaactgg ccgtgcagaa
     6061 agtgcttgtc agcgtcagcc gtcttgtggc cggccggttt gtggcggagg acccggccga
     6121 tttgaaccgt tacggactgg cgccgcccgc cgatgaactc acaattggcc ttgcggggca
     6181 ggacggaatc agcaaaacac tgatgcttgg aaacgagtcg gaggacgggg gcttctatgc
     6241 catgatcaag ggacaggaca ttattttcac actggaccgc gagatcaggg acgcggcatt
     6301 tcccgccacg ctcttcagcg tgcctcctgc gggagaaacc ccgtgaccac cggccgtcag
     6361 gcgggccgca ctttatcgcg gccggtgcac gcgcttaagc gctgcttccg ctgcttcctt
     6421 ctgatcgtgc ttctgctgct ggcggcgggc ctctatatga accgggtcgg actcccggac
     6481 gcggtgaaga accgcgtggt ccgcgaactg gccgcccgcg gcgtgcgcgt ggaggtcgcg
     6541 cgcgttcgct gggaattgct ggaaggcatc gtgatggacg accttctcta ttttgccggc
     6601 gcgctcgacc ccgagccggt ggtgcgggcg gaagaggcca ccgtcaatct gcgtcccgcc
     6661 gcctggtttc ggggacgtca tggcgtgcgc ggtttcgcca tccgcaatgc gcgcctggga
     6721 acgggcgagg gcctgtccgg catccacgca tccgtgcggc tggaggacga gggcctgcag
     6781 atccgggatc ttttcgccga ttacggcggg ctgaaaataa caggagaagg tttcattgcg
     6841 cggcctcagg ccaatatgaa cggtgcgctg atgcaggcat ggcgtcagat tttcccgccg
     6901 tcggacaaaa cggagaaaac cccggcgctt cgcgtcctgc ttgaggacct gaaggccgtc
     6961 cagttcgcgc agggatcttc cgcgcagatc atgctggtgc ttcatccgga tgatctgaat
     7021 gccaacgagt tgaaaatcga ggcacgcggc ggcggactca ccctccgggg cgtcaatctg
     7081 gagggctggc ggatgcaggc ggctctcaag aatggccgct tcacgcttgc acgcctgtcg
     7141 gtcgcctccg gacagcaggc tgtgcgggca gccggattcc tcgatctaac cgacggcacc
     7201 gccgcctgca aactgttcag cgacctgcct ccgtatgtgc ttctggcgct ggccccggtc
     7261 tcgctgcggc agactctgga ctcactcgaa atccttcccg gcgaagccct cgaattcacc
     7321 gccgatgccg ggcctgcacc ggtcggcgag ctgcttgatg ttgttcacgg ctcgctggca
     7381 ctgaaggatt cccgcctgct cggcctgccg gtggcctcaa cgtcgctgga gttcagccgc
     7441 gaacatcagg tgcttgcggt aaccaatctg caggcttccg tagggctgga gagccggagc
     7501 ggccccctga cgggatatgc ccgctatgac ctggaatcgc aaacctacag cggccggatg
     7561 aacaccggct tcgatcccgg ctggatgtgc cccctcgtaa gcaccggcaa ggcgcgtgtt
     7621 atcagcggtc tgtttttccg cgagaatctt ccgcagactt ctttcacgtt ctccggccag
     7681 cgaggcgacg acggagcgcc cctgcaggtt gagggctctg ttcggggcag gaatttcagt
     7741 tttcgcggga cctatttcac ctccgtggaa tccaaactgg tcatgaccaa cggggtgctc
     7801 attctggatc cggtcaccgc gcgtcgcggc agccgcgcct gccgcggccg gattgctttt
     7861 gatttccgga acgatatcgt ggatgtggac gtggagagca cttttgatcc cgccgccacc
     7921 cttcgcatta tgggcccaac ccttgaacgg ctgtcccgcc cgttccgctt tgacggatat
     7981 acccgcatct cggcgaaggg aagcattgac tatgaaacgc tgaccaacat gaaccttgag
     8041 gcgcatgtgg tggcttccaa catgacctat cagcacgtgg ccgtggaaag ctgcgccttt
     8101 gatctgcttg cgaaaggccg ccgttttgaa gtcagaaatc tgcagggggt tctgtacggc
     8161 ggccggctgg aaatgtcgcg cgcggttatg gagtcgctcg cggattctcc cctctggcgc
     8221 tatgaactgg cgctttctct ggccggcgcg gattttggaa gactgatgca ggcgtatcgc
     8281 cgggtgggag cggatcaccc ctatgagggc aggctcgatt tcatgctggc agtcgccgga
     8341 gagaccggcg aacagatgct cctgcggatg cgcggacggg gagacattca tatctccgag
     8401 agccgtctgc tccgcattcc gctgttcgga ggactggcga agattctgtc cgcgctgtat
     8461 ccgaatttcg gaattgtatc aaagaacgat cttaccgcgg acttcaagat tggcggcaat
     8521 tccattcgca cgcagaacgc gcggcttctg ggacatatca tcagcgtgca gggctccggc
     8581 cgctatcatt ttacaaacaa tctggatttc aaagtgcgtg tgcgaatgct ccgcgagggc
     8641 cggctggccg ccctgctgga ctggctgacc ttcccggtga ccaaactgct ggagtttcac
     8701 ctgagcggtc cgctggagca gccggtctgg cggcccgaca atcttccgaa ggagctcttc
     8761 aacatgctga aatctgtcat tccgggcatt ggcgatgaag caaaacgctg accatgcctg
     8821 gcatgtcctg acggttgaac tccccgagga acacgcagaa gccctgcagg agtggatccg
     8881 cgaccggtat gggctggaac cggtcctgct ggagcgcgcc ggcgcccgcg gtcagtggat
     8941 cgaaacatac ttccctgacg cctctgccgc gcgggaagcc gccgtggaaa ccgccgaagc
     9001 cttcccggtg cgcgcgacgg ccgtccggct gctcgaagcc agggactggc agaatttctg
     9061 gcgtcatcat ttcatccgcc gggaaatcgg ccggtcgctc ctgattgttc ccgactggga
     9121 gcagaacggc gatacgggcg gccgagccgt catccggctg gaaccgggcc tcagcttcgg
     9181 aacgggagat aatttcacaa cgcgcttctg cctggaaatg attgatgaac tcttcgcgaa
     9241 acgcgttccg gaaagcttcc tggatgtcgg cgccggaagc ggcattcttt ccattgccgc
     9301 ggcgaagctc ggctgtccgc gcattatggc gctggagaat gacgccctga gcgtggccag
     9361 cgcccgcgag aatctcgagc gcaacggagt cacaagcctc gttactattg aggcgcatga
     9421 cattctttcg ggatgggacg ggggccgata cgagtttata tgcgccaaca tcatctccgg
     9481 cgtgctgacc cgggcggccc cggtgctggc gcagaccgcc tcccgcctgc tggttctatc
     9541 gggaattctc gaatgggaat gcgacggggt cgcggagata tatcagcggc tcggcggccg
     9601 cgaggtcatt cgcgacggtg atgggcagtg gtgcggtctt gtgtttgaat tcaactgatc
     9661 tgcgggcctc gaaccggcgg ccgcgcgctg tcggttgttt acttcctgcg ggattgaggt
     9721 actatggccg gactatgagt tttgactgga acattctgat gctcggcacg gtgatatttc
     9781 tcgcccgtat tatggatgtt tcgctcggca cactccgtat tatcagtgtg gtgcaggggc
     9841 ggacatggac ggcgtttttt ctgggatttt tcgaggtcgg catttggatc accatcatca
     9901 gcgcggtgat gcattacatt aatgagcagc cgattctggg ctttttctat gcgctgggct
     9961 ttgccgcggg cagcgtggtg ggcatcaaga ttgagcggcg gattgcttcg gggcacatcg
    10021 ctctgcgact catcagctat gagcatggcg aagccatggt ggaggccatc cgcagggccg
    10081 gctacggcat gaccgtttat gagggcatgg ggaagcacgg cccggtccgg gaacttttcc
    10141 tggtgtgcct gcgcaaggac agatccgcca ttctgaagat cgcgaaaagc attgatccgg
    10201 acgtgttcag caccacctcc ccggccgac
//
LOCUS       DAOQWR010000093        10198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7036_length_10198_cov_4.999507, whole genome shotgun sequence.
ACCESSION   DAOQWR010000093 DAOQWR010000000
VERSION     DAOQWR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_10198_cov_4.999507"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKK36_08135"
     CDS             complement(<1..892)
                     /locus_tag="PKK36_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94562.1"
                     /translation="MILKRHLILLLIPVTCFAADEPVISLTPLTVYPEGGAAMSGNAL
                     DGFVPVPPVVCLNSQGGFGRQTDISIQGSSFSGAGLTVAGIALRNPQTEHFHAELPFP
                     DFIFLEPAAATGLEQALNGQGFLAGSVALSLREIIPAARLHLGAGDLNINRQAIGIQD
                     ALSAGVGWGLFAERMEAGGRDYDDNDLSSEGGGGQIQLRSEMWQVDLLAAHRRKEFGA
                     RGFYGVSPALFAEEKTKDTLVLATARRAEDESWIRASASWQSFEDDYRLFWTLPGIYE
                     NRHRSDLAAALLDGRADGQGS"
     gene            complement(889..2235)
                     /locus_tag="PKK36_08140"
     CDS             complement(889..2235)
                     /locus_tag="PKK36_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNR94563.1"
                     /translation="MKKNETAMTVLISGGSVLQHLPGGKVNVLENHDVLVQGDSISAV
                     EPAGTMDRNIAKKIIDARGRLVMPGLINCHSHVPMVIFRGLAEDVSLDKWFNEYMWPL
                     ESNLQEEDVYWGMLLGLAEMIEAGVTTVADHYFFMDQAAEAVKSAGTRAALGWAMFGS
                     QGPAIIDRTSEFITKWNGAAEGRITAWMAPHAPYTCDDVFLRAAAARAKELKVGIHIH
                     AAETKGQTEASVKARGITPIRVLEETGILDSRALIAHAVGATEQDIEILRMHSSAVAH
                     CPKTYLKLAMGTAPVVDFLKADVTVGLGTDGAVSNNTLDVWESMRLMAMTQKQAHEPE
                     AMPMDQVLSVAGPGSAEAVGLKNGIGRIEPGMLADLIVVDLDGTHLQPMHSVKASLIY
                     SVRASDVRTVVVNGNLLMEDRRLLTIDKQEVVKQVRQSMKRLSQRVPASRIQVYKP"
     gene            complement(2232..4142)
                     /locus_tag="PKK36_08145"
     CDS             complement(2232..4142)
                     /locus_tag="PKK36_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR94564.1"
                     /translation="MHQSHHLSAENGAAHLPARRLWSYALLVFLAALAYRTVFLFEAA
                     SKPDFELVYMDMEYNLEWARCMSTGSWPEPYARLQNEPYFRAPLYSMFLAALLKISSG
                     NLWFIRVVQILLGSFSCMLAYLLACRLYGLRTGLITGFLCAGYWVMAYFDTEFLLPVL
                     LVFLALAAFCLLSLAMDRRSLFLACLSGLMFGLFSVTRPNMLAFFPVPVLWIFLAARS
                     WPRRRQACFALILAAGFALPPLAATVRNRVTGGDWVVVASQGGVNFYIGNNPRSNGMQ
                     AVVPGTRATWWGGYEDTVATAEAAMGRPLRPSEVSDYWYRRALEYIRSSPGHWLKLTA
                     RKCAALIGSVEIPNNEPYEARRGEFISQSAVPLNFGVLLALFVVSFPRLLKGEGGSSE
                     SRRSMIALILMFMAVYSATIVAFFVTGRYRVPLVPFVAMGAAAALVQWIDFLRARRWG
                     KALCMAAVAILLFPMLQLDLLHVRRGTMGFVRLTAAQDSLEAGRTDEAIAALEKLREE
                     NILAVPEVDVSLARAYLQRGRPEDRAAVLALSQNSLERHPDNAELLWYAAVGHVEGRN
                     WAGAEEMIRRYLAQEPNDVRALHLACLSALQQDRRADAEALYRRAAGLESNHPAVRDM
                     KKMLERAREPSP"
     gene            4409..5560
                     /locus_tag="PKK36_08150"
     CDS             4409..5560
                     /locus_tag="PKK36_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94565.1"
                     /translation="MNDVPLYRLSVISKGARAQFAIGVSLISILPLLMVGYLYTFFRN
                     EATLYSVQTLAVLTTATLVGLTGFIILRKYPINIIRLREYLEQMIRGELPDQISLVKE
                     EDDIQMVEKCLNMILLQLHERLEILKTEKQKLQNQIYQLQKMESLGVMAAGVAHDFNN
                     LLTGISGLTKLMNGHIRDAEAQSRLKEIESLVSRASELTRQMLVYAGRGRFALEKVDA
                     GGLVKDMAHLLNSTVARGVTTQYEAAETPTVVRADPTQLRQVIMNLVINASDAMEGRG
                     GTIQVRTSCVRLRREDLLNCIVHGTAPAGNAACIEVVDQGPGIPADQMPRIFDPFFSS
                     KPKGRGLGLAVVLGIVHSHKGAVVVDSEAGRGARFRVLLPLAEEAGIGS"
     gene            5613..6413
                     /locus_tag="PKK36_08155"
     CDS             5613..6413
                     /locus_tag="PKK36_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNR94566.1"
                     /translation="MGKIRISVIQTSSGLNQSRNLQAVGEMIAGMASSDLIALPEVFS
                     LRGNDGHCRKAATTLNSHPAVAHARNWCSEHRCHVLLGSVIERQADKVFNTSVLLDRK
                     GEIAAVYRKIHLFSADLEDGTRIDESELYTPGDEPATCELDDWICGFSICYDLRFPEL
                     FRAYADLKADIVFVPSNFTAATGKDHWEILLRARAIENQCWIIAPNQCGINPDTGVES
                     YGHSMIINPWGRIAAEAGTEPCVITFELDPQAAKDARKRLPVLTHRRM"
     gene            complement(6564..6941)
                     /locus_tag="PKK36_08160"
     CDS             complement(6564..6941)
                     /locus_tag="PKK36_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNR94567.1"
                     /translation="MSMDGQMVKGYTDLIVLALLRRLGPVHGYALRAEMRDLSHQIFH
                     MSFGSLYPLLHDLEKRRLLTSRIHPAGEVRTQRRFSITKAGEDYLQHQIQKWEMFSQA
                     LESVIRKRGNRKQLAQAQIRKLL"
     gene            complement(7799..8437)
                     /gene="leuD"
                     /locus_tag="PKK36_08165"
     CDS             complement(7799..8437)
                     /gene="leuD"
                     /locus_tag="PKK36_08165"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014371533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNR94568.1"
                     /translation="MEAFSKHKGLVAVLDRANVDTDQIIPKQFLKSIRRTGFGEALFY
                     DWRYLDDGSDNPDFELNHPRYKGASVLVVRNNFGCGSSREHAVWAVAQYGLKVLIAPW
                     REASGQRISGFADIFRNNCTKNGVLTVELSEDEVSKIMDLVRASEGLQMTADLPAQKL
                     IFHLPKELVFSFDIDAGLKEYLLKGLDDIGSTLGYEADISAFEKKHDPQMAK"
     gene            complement(8440..9846)
                     /gene="leuC"
                     /locus_tag="PKK36_08170"
     CDS             complement(8440..9846)
                     /gene="leuC"
                     /locus_tag="PKK36_08170"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNR94569.1"
                     /translation="MGKTLYEKIWDRHVVKDLPGGFSLLYIDRQLVHEVTSPQAFEGL
                     RMTQRKVRHPEMTFATMDHNVPTTPGREITDEVSRAQIEALSKNARDFGIRLFDLDDP
                     NIGVVHIIGPELGITLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLRQK
                     PSKTFKVEFSGKLQPGVTSKDMILKLIGIIGTAGGTGYVIEYMGEAVRRLSMEARMTI
                     CNMSIEAGARAGMIAPDETTFEYIRSGDRPYAPRGEQLEKAIGYWQTLPSDPDAKFDR
                     ALGIDVNKLVPQVTWGTSPGMVTDVTAKVPGLNDVSGYSATDVEKALEYMGLKPGTPM
                     TDIKVDTVFIGSCTNGRIEDLRQAASIMKGRKVASGLRVLVVPGSGAVYRQAEAEGLG
                     QIFRDAGADWRLSGCSMCLAMNPDKLAPGERCAATSNRNFEGRQGKGGRTHLVSPLMA
                     AAAAVEGHFVDVREWVKG"
BASE COUNT         2208 a         3053 c         2907 g         2030 t
ORIGIN      
        1 gactcccctg cccgtcggcg cggccgtcaa gcagtgcggc ggccagatca gaacgatggc
       61 ggttttcata aatgccggga agcgtccaga acagccggta atcgtcctca aaggactgcc
      121 atgaagccga tgcgcgaatc cagctttcat cctcggcgcg acgcgcggtg gccaggacca
      181 gtgtgtcctt cgttttctcc tcggcgaaca gcgccgggct gacaccataa aaaccgcgcg
      241 cgccaaactc cttccggcgg tgggccgcca gcagatcaac ctgccacatt tctgaacgga
      301 gctgaatctg cccgccgccg ccctcggatg acaggtcatt gtcatcataa tcccggccgc
      361 cggcctccat gcgttcggca aacaaccccc agccgacgcc ggccgaaagc gcatcctgaa
      421 tgccaatggc ctggcggttg atattcaggt cgccggcgcc gagatgcagt cgggctgcgg
      481 gaataatctc ccgcagggac agagccaccg atccggcgag gaagccctgc ccgttcaacg
      541 cctgttcaag accggttgct gcagcaggct caagaaaaat aaaatcgggg aacggaagct
      601 ccgcatggaa atgttcggtc tggggattgc ggagcgcaat gcccgcgacg gtcagcccgg
      661 cgccgctgaa ggaactgccc tgaatcgaga tatccgtctg ccggccgaag ccgccctggg
      721 aattcaggca aacgacgggc ggcaccggaa caaagccatc cagcgcattg ccggacatgg
      781 ccgcgccgcc ttcgggatac accgtcaatg gagtcagact gatcacaggc tcatccgccg
      841 caaagcaggt gacgggaatc agcaggagaa tcagatgtct cttcaaaatc atggtttata
      901 gacctgaata cggctggcgg gcacacgctg gctgaggcgc ttcatggact gccgaacctg
      961 tttcacaacc tcctgtttgt caatcgtcag aagcctgcgg tcctccatca gcaggttgcc
     1021 gttcacaaca acggtgcgca catcggacgc gcggacagaa taaatgagcg aagccttgac
     1081 gctgtgcatc ggctgcagat gcgtgccgtc cagatcaaca acgatcaggt cggccagcat
     1141 tccgggttcg attcggccga ttccattttt caggccgacg gcttcagcgc tgccggggcc
     1201 ggcgaccgac aggacctgat ccatgggcat cgcttccggc tcatgcgcct gcttctgcgt
     1261 catggccatc aggcgcatgc tttcccagac atccagcgtg ttgttgctga ccgcgccgtc
     1321 ggtgcccagc ccgaccgtta cgtccgcctt cagaaaatca acaacggggg ccgtgcccat
     1381 cgcgagcttg agataggttt tgggacagtg cgccacagcc gagctgtgca ttcgcagtat
     1441 ttcaatatcc tgctcagtgg ctccgaccgc atgagcaatg agcgcccgcg aatccaatat
     1501 gccggtttcc tccaaaacgc gtattggagt gatcccccgc gctttcacag aggcttcggt
     1561 ctggcctttc gtttccgccg cgtgaatatg aatgccgacc ttcagctctt ttgcgcgggc
     1621 ggcggccgcc cggagaaaaa catcgtcgca ggtgtacggc gcatgcgggg ccatccatgc
     1681 tgtgattctt ccttcagccg ctccgttcca ttttgtgatg aactccgagg tgcggtcaat
     1741 gatggccgga ccctggctgc caaacatggc ccagcccagc gcagcgcggg tgccggcgga
     1801 tttcacggcc tcggcggcct gatccatgaa gaaatagtgg tcggccacgg tggtcacgcc
     1861 ggcctcgatc atctcggcga ggccgagaag catgccccag tagacatcct cctcctgcag
     1921 gttgctctcc agcggccaca tgtattcgtt gaaccacttg tcgagcgaca catcctcggc
     1981 gagcccccgg aaaatcacca tcggcacatg cgaatgacag ttgatcagcc ccggcatcac
     2041 cagcctgccg cgggcatcaa tgatcttctt tgcaatattc cggtccattg tcccggccgg
     2101 ttccacggcg gaaatgctgt cgccttgaac cagcacatcg tgattctcca gcacattcac
     2161 ctttccgccc ggcagatgct gcagaacgga tcctccgctg atcagaacgg tcattgcggt
     2221 ttcgtttttc ttcatggcga cggctcccga gccctttcca gcattttttt catgtcgcgc
     2281 acagcgggat gatttgattc cagccccgcg gcgcggcggt acagggcttc ggcatccgca
     2341 cggcggtcct gctgaagggc ggacagacag gccagatgaa gggcgcgaac atcattcggt
     2401 tcctgcgcca gatagcgccg gatcatttct tccgccccgg cccagtttcg cccttcgaca
     2461 tggccgactg cggcatacca caacagttca gcattgtctg gatggcgctc aaggctgttc
     2521 tgcgaaagag ccaggacggc cgcgcggtcc tcaggccggc cccgctgaag ataggcacgg
     2581 gcaagactga catccacctc cggcacagcc agaatattct cctccctgag tttctcaagc
     2641 gcagcaatgg cctcatcggt ccggcctgct tcaaggctgt cctgagccgc agtcagccgg
     2701 acaaacccca tggttccgcg gcgcacatgg agcagatcaa gctgaagcat gggaaacagg
     2761 agtattgcga ccgcggccat gcacagcgct ttcccccagc gccgggcgcg cagaaaatca
     2821 atccattgca ccagcgcggc ggccgcgccc atggcgacga agggcacaag cgggacacga
     2881 tatcttccgg ttacgaaaaa ggccacaatg gttgcggaat agacggccat aaacatcagg
     2941 atcagcgcaa tcattgagcg gcggctttcc gatgagcccc cctctccctt cagaagccgc
     3001 gggaaagaca caacgaagag tgccagcaga accccgaaat tcaacggcac ggccgactgc
     3061 gagataaatt ccccccggcg cgcctcatag ggctcattgt ttggaatttc cacggatcct
     3121 atcagggccg cgcatttgcg ggcggtcagt ttcagccagt gaccgggact gctgcggatg
     3181 tattcaagag cgcggcgata ccagtagtcg gagacttcag acgggcgcag cggtcgcccc
     3241 atggcggctt cggctgttgc gacggtgtct tcatagcctc cccaccatgt ggcgcgggtg
     3301 ccgggaacca cagcctgcat gccgttagaa cgagggttgt tcccgatgta gaaattgacc
     3361 ccgccttgag aggccacaac cacccagtcg cctcccgtga cgcggtttcg cacggtggcc
     3421 gccagcgggg gcagcgcaaa gccggccgca agaatcagcg caaagcatgc ctgccggcgg
     3481 cggggccagg atcgcgcagc gaggaatatc cacaaaaccg gcacggggaa aaaagccagc
     3541 atattgggcc gggtgactga aaagagcccg aacatcagcc cggacaggca ggccagaaaa
     3601 aggctccgcc ggtccatcgc cagggaaagg aggcagaatg cggccagagc gaggaacacc
     3661 agcagaaccg gaagcaggaa ttccgtgtcg aaataggcca tgacccagta tcctgcgcac
     3721 aggaaaccgg tgatcagccc ggtccgcagc ccgtataagc ggcaggccag cagatacgcc
     3781 agcatgcagg aaaacgatcc aaggagaatc tgcacgactc gaataaacca gaggtttccg
     3841 gaagatattt tcagcagcgc cgcgaggaac atggagtaga gcggagcccg gaagtacggt
     3901 tcattctgca ggcgggcata gggttcgggc cagctgccgg tgctcatgca gcgggcccat
     3961 tccagattgt attccatgtc catatagacc agttcaaaat ccggtttcga agccgcttca
     4021 aacagaaaaa cggttcgata tgccagcgcc gcaagaaaca caaggagcgc ataagaccac
     4081 agccgccgcg ccgggagatg cgccgcgccg ttctctgcag acagatgatg actctgatgc
     4141 ataacaatcc atagttccag atttccggcc cgccagcatt gagggattcg ccttcttccc
     4201 gtcttcttct tcgcaggcgg tatctgcaac tgtctcgata tcaatgaaga gcgtagcggg
     4261 tcactcatgc cgtgtaaaga cgcaagactg accgaaggga gtctcctgat gggccgcaaa
     4321 gcacgaagga agcgatctaa atgtcttgat ccccgggagt taaactgata tacaaaaaat
     4381 catgagattg tcttcgaagg ggggctttat gaatgacgtg ccgctttatc gtctttccgt
     4441 gatctcaaag ggagctcggg cgcagtttgc cattggcgtt tcccttattt ccatcctgcc
     4501 gctcctgatg gtgggctatc tgtacacctt cttccgcaac gaggccacac tctacagcgt
     4561 gcagacgctg gctgttctga ccaccgcaac actggtggga ctgacgggat ttataatcct
     4621 tcggaaatat ccgatcaaca tcatacggct gcgcgaatat ctcgagcaga tgattcgagg
     4681 cgaattgcct gatcaaatca gccttgtgaa agaagaagac gatattcaaa tggtggagaa
     4741 atgcctgaac atgattcttc ttcagctgca cgaacgcctt gaaatactca aaacggaaaa
     4801 gcagaagctc cagaatcaga tctatcagct tcagaaaatg gaaagtctcg gggtcatggc
     4861 cgccggagtc gcccacgact tcaacaatct gctcaccgga atcagcgggc tcacgaaact
     4921 aatgaatggt cacattcggg acgcggaggc ccagtcccgc ctgaaggaaa tcgaatcact
     4981 ggtcagccgg gcctctgaac tcacccgtca gatgctggtg tatgccggac gcgggaggtt
     5041 cgcactggaa aaagtggatg ccggaggact ggtgaaggac atggctcatc tgctgaattc
     5101 cactgtggcc cggggagtga ccacacaata cgaggccgct gaaacgccaa ctgtagtccg
     5161 tgcggatcca acccagctca ggcaggtgat catgaatctc gtaatcaacg cttcggacgc
     5221 catggaggga cgcggcggaa ccattcaggt gcgcacgtcc tgcgtgcggc tccgccggga
     5281 ggatctcctg aactgcatcg tgcacgggac cgcgccggcg ggcaacgccg cctgcatcga
     5341 ggtcgtggat caggggccgg gcattccagc ggatcaaatg ccgcgaatct tcgatccttt
     5401 cttcagcagc aagcccaagg gacgcgggct gggactggcc gtggtgctgg ggatcgtgca
     5461 cagccacaag ggcgccgtgg tggtggacag cgaggcggga cgaggagcgc gtttccgggt
     5521 gctgctcccg ctggccgaag aagcgggaat cgggtcctga accgcggttt gagctttcgc
     5581 ggccggatca attcccctat catttctccc tgatgggaaa aatcaggata tcggtaattc
     5641 agaccagttc aggactcaat cagtcccgga atctgcaggc cgtcggggaa atgattgcgg
     5701 gcatggcgtc atccgacctt atcgctctcc cggaagtttt ctcactgcgg ggtaatgacg
     5761 gtcactgccg caaggcggcc acaacactca acagtcatcc ggccgtcgcg cacgcccgga
     5821 actggtgcag cgaacacaga tgccatgtcc tgctgggaag cgtgatcgag cggcaggctg
     5881 ataaggtgtt caacacttcc gtgctgctgg accgaaaagg ggaaatcgcg gcggtttaca
     5941 gaaaaattca tcttttctcc gctgatttgg aagatggcac acgcattgat gaatctgaac
     6001 tgtacacgcc cggggacgag ccggcgacgt gtgaactgga tgattggatc tgcggcttct
     6061 ccatctgcta tgacctgcgc ttcccggagc ttttccgggc ctatgcggac ttgaaagcgg
     6121 atattgtgtt tgtcccctca aacttcacag cggccaccgg aaaggaccac tgggaaatcc
     6181 tgctgcgcgc gcgcgccatc gaaaaccagt gctggatcat tgccccgaat cagtgcggga
     6241 taaatcccga cacgggggtc gaaagttatg gccacagcat gattattaat ccatggggca
     6301 gaatcgcggc cgaggcagga acggaaccct gcgtcataac gttcgaactt gacccgcagg
     6361 ccgcaaagga cgcccgaaag cgccttcccg tgctgacgca ccggcggatg taaacgcatt
     6421 gatcatttcg ccatcacagc acttccgccc ctgccgcctc caccagtctt caatatatcg
     6481 gtatccgtta tatcggtttc cgatatattt acggagaatg atattgcgaa tgcggcgggt
     6541 gcaacttgcg gattatgacc ggatcacaac agcttgcgaa tctgtgcctg cgccagctgc
     6601 ttcctgttcc cccgcttgcg gatcacggat tccagcgcct gcgagaacat ctcccatttc
     6661 tgtatctggt gctggagata atcctcgccc gccttcgtga tgctgaaccg gcgctgcgtc
     6721 ctcacctctc ctgccggatg aatgcggctg gtcagcaaac gccttttttc aagatcgtgc
     6781 agcaggggat aaaggcttcc gaaagacatg tggaatatct gatgggacaa atcccgcatc
     6841 tcggcccgga gcgcatatcc atgcacaggg cccagccgcc ggagcagtgc cagcacaatc
     6901 aggtccgtgt accccttcac catctgccca tccatgctca ttttcacgcg cccaatatat
     6961 cggataccga tatattggca agaccaatcc gaccgcgggc gaaatcactg gatgcccggc
     7021 cggtaatgcc aactcgcatt gcgcgcctcc attcgcgatt gctccaggca tatctcaata
     7081 atagtctccg acgtatatta tatcggtatc cgatatatcg gttactgata tattgcgata
     7141 tcgcggcaag tgatttcagt gtatgggccc cggacagcaa ctgttaaaca cagccacccc
     7201 acaagccaaa tgcgagctgt ttcaggggca cgggcattct gcccgccttc ccatgtggcc
     7261 gagctggagg ccgacgttcc cggagccgca tagatcaggt cacgttcagt tcgctgcaga
     7321 agacctcaaa gggcagcacc cggacgcccg tcgcagcgcc acccgacagt tatatttcgg
     7381 tttccgatat atcggtaaac gatatattgc ggtgattatg gcgagcacat tgagcagaag
     7441 attccaaagt cgagcgcggt ttaactttgc tgaagcggga tgacttaagg agcgcgggca
     7501 tcctgcccgc tttcccatgt gaccgagctg gaggccgacg ttcccggagc cgcatagatc
     7561 aggtcacgtt cagttcgctg cagaagacct caaagggcag cgcccggcgc ccgttcaagc
     7621 gacgtccact ggtcatattg cggtatccga tatatcggtt aatgatatat tactgcaacc
     7681 atcacaaagt cgttgctgaa gcaaaaagga cttcagggga agctggcagg atgcacgcgc
     7741 ccctgccccg cgtcggatcg tcgaggcgtc ccaccaggcc gattactgat tcaacgaatc
     7801 atttcgccat ctgcggatcg tgctttttct cgaaggcgga gatatccgcc tcatagccga
     7861 gggtgcttcc gatatcgtcc agacccttca ggaggtactc cttcaggccg gcatctatat
     7921 caaagctgaa aaccagctcc ttcggcaggt gaaagatcag tttctgcgcg ggcaggtcgg
     7981 cggtcatctg cagcccttcg ctggccctga ccagatccat gatcttcgag acttcatcct
     8041 ccgaaagctc caccgtcagc acgccgtttt tggtgcagtt gttgcgaaag atgtcggcaa
     8101 agccggaaat gcgctggccg gaagcctcgc gccagggcgc gatgagcact ttcagcccgt
     8161 actgggccac ggcccagacc gcatgctcgc ggcttgaacc gcacccgaaa ttgttgcgca
     8221 ccaccagcac cgacgcgccc ttgtagcgcg ggtggttcag ctcgaaatcg ggattgtcgc
     8281 tgccgtcatc cagatagcgc cagtcgtaaa acaaagcctc gccgaagccc gtcctgcgga
     8341 tggatttcag gaactgcttc gggatgatct gatccgtgtc cacgttggcg cggtccagca
     8401 ccgccaccag ccctttatgc tttgagaacg cttccatgat taacctttca cccattcccg
     8461 gacatccaca aaatggcctt ccaccgccgc ggcggcggcc atcagcgggc tgaccaggtg
     8521 tgtgcggccg cccttgccct gccggccctc gaagttgcgg ttggaggtcg cggcacagcg
     8581 ctcgcccgga gccagcttgt ccggattcat tgcaaggcac atgctgcagc cggagaggcg
     8641 ccagtctgcg cccgcatcgc ggaatatctg ccccagccct tcggcctccg cctgacggta
     8701 gaccgcgccg gaacccggca cgaccagaac ccgcaggccc gaagccactt tgcggccctt
     8761 catgatggaa gccgcctggc gcagatcttc aatgcggccg ttggtgcagc tgccgatgaa
     8821 cacggtgtcc actttgatat cggtcatggg cgtgccgggt ttcaggccca tgtattcaag
     8881 ggccttttca acatcggtcg cggaataacc ggacacatcg ttcaagccgg ggactttcgc
     8941 cgtaacatcc gtgaccattc cggggctggt gccccaggtg acctgcggca ccagcttgtt
     9001 cacatcaatg cccagcgcgc ggtcgaactt ggcatccgga tccgaaggaa gcgtttgcca
     9061 gtacccaatg gctttctcaa gctgctcgcc gcgcggagca tagggccggt cgccgctgcg
     9121 gatatactca aaggtcgttt cgtcgggcgc gatcatgccc gcgcgcgcgc ccgcctcgat
     9181 gctcatattg cagatggtca tccgcgcctc catcgaaagc ctgcgcacgg cctcgcccat
     9241 gtattcgatc acatagccgg tgccgccggc ggttccgatg atgccgatca gcttcaaaat
     9301 catgtccttg gaggtgacgc ccggctggag cttcccgctg aactccacct tgaaggtctt
     9361 ggagggcttc tggcgaaggg tctgagtggc cagcacatgc tcgacctcag acgtgccgat
     9421 gccgaacgcc agcgccccga acgcaccgtg cgtcgcggtg tgcgaatcgc cgcagacaat
     9481 agtcatgccc ggcagggtaa ttcccagctc gggaccgatg atatgcacaa ccccgatatt
     9541 cgggtcatcc aggtcaaaca ggcggatgcc aaaatcgcgg gcatttttgg agagcgcctc
     9601 gatctgcgcg cgggacacct cgtcggtgat ctcgcggccg ggcgtcgtcg gcacgttgtg
     9661 atccatcgtc gcaaaggtca tttccggatg gcgaaccttg cgctgagtca tgcgcaggcc
     9721 ttcaaaggcc tgcggactgg tcacctcgtg caccagctgc cggtcaatat agagcagaga
     9781 aaaaccgccg ggcaggtctt tgacgacatg ccggtcccat attttttcgt ataatgtttt
     9841 acccatagcg gagtaaatgt accagatgac tttgaaacta caagtccgct gttcatttcg
     9901 cgatgcgata aaacagagaa cattcagtcg ggaaaaaaga aatccagcgc cggacattaa
     9961 aatagtacta ttttagtact atttcatttt ttgggcgttt ttgaggcatt tttaagcgtt
    10021 tttggcctat ttttggccaa tttgggccgt tttttggcat tttttcaatg ttttgggccg
    10081 gtttcagggc ccccggggga tccctcgaaa aatcacattt tgagcctgcg gccaagggcg
    10141 tacatggcga tgctgccggc gacgggccac atgttggtgg tgtgggagga cgacgcgg
//
LOCUS       DAOQWR010000094        10170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7058_length_10170_cov_4.882551, whole genome shotgun sequence.
ACCESSION   DAOQWR010000094 DAOQWR010000000
VERSION     DAOQWR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_10170_cov_4.882551"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..10158)
                     /locus_tag="PKK36_08175"
     CDS             complement(<1..10158)
                     /locus_tag="PKK36_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR94570.1"
                     /translation="MTAQGSAAVTNRGVCYKTSAGVAITDNKTQSGSGTGAFSVNLSS
                     LSVNTKYYFKAYAINTAGTTLGSELSFTTLANTPSAPTVNNPTASSLDITINENSNPA
                     ATEFAIQRTSDSNYLQANGSWGASAVWQNKATWGSPKTATGLSAATTYSIRVKARNGE
                     NVETAFSSTADGTTCFDSVTGVYVNPTNETNFTINWSAVSGADGYVVDVSTEEDFGGG
                     GGGSTNNIFISEMCDPQNNYTSDRYIEIFNAGSASVDLTGWSVVAVGNSSDIHTWTLS
                     GTIGSSNALTCGDSSNTQFTPDFSDSSWSSDNTSWNGKVGDGARLVRSGTTIDNTKTT
                     ADAFENKTYVRNAGIGTGVTTYASGEWSATAVSDAGSGASTPGSHDCTSVPSGGSGGG
                     GLNCIPGYTNREVASTSLSVTGLTGGVTYYFRVTATNANCSATSSTGSVTTTSAATSP
                     EIAVLGTNLAEIVSGDNSPAVSDGTDFQLWEASTTHDHIFTITNSGNDTLTLSGQPTL
                     SGDAAFSRQANVGDLTLAPGQAETFTIRFTAPSNPGGVYTSLVTVASDDTDEGTYTFA
                     IRAEVHCFTEVTGVYASQTNAQDFTAAWSAVTGADGYILDVATNADFSSAGGSASDLF
                     ISEYIEYGNEKYIEIYNGTGGSVDLSNYALVLYANGATSHTASNTLSGTLADGDVVVY
                     KNSSSTNTVGTDSSSVNYNGDDAVALWKSTSSSWVDIFGRIGEDPGAQWTDGSFSTAD
                     ATLVRKSTVTGGVTSNPGSGFPTLGTEWDLYDATDESHLDDHTFSGGSGGSMVPGFDD
                     LPVSGTSQIVTGLTQGITYYFRVMATNENCESSWSSTQQVTTVVSADTAGPTFSNFAV
                     TNSTGTVVLDRHFSGFPIFVDIADSTTGVRWTTATQPFFTITNPSNSEVVGPTTFTTT
                     GHADRETACTATGTVPDIASSFVAGTWKVGVWAEDMNDYAGSDTFTFTAADDDDAYPN
                     IYSVHLSSTDEGADAVGSGALLAGTGWWLTGMVNDAGSGVDTNSFPGYFLLYDFNGDL
                     QYSNQMIMKFDDGAATVQGQIQHSALSGLSSAPAGVWTARVYVTDNDADGWTGDSLTS
                     VSNIPFTVVYSCPAGPGTLWADPTNVFDYTANWSAVGGVSDYIINVYSNYAAADELFF
                     SEYIEGSGYNKYLEIYNGTGSDVDLSDYVIHQFNGGSPTPSFTMTLTGTLADGDVYVI
                     ANDSASAWGGSPDLVTNSSVMAFNGDDALALSNTALNTYCDIIGQIGTDPGTEWGSGD
                     TSTSDNTIRRKSSVTEGDTDGTDAFDPATEWDGYAQDTVSDLGSHTVSGGSGTSEWVP
                     GFSNLLVSSSTSQSITGLVPNTPYYFEVASIGADCTGSFSAVETVYTRPVMDPTAASA
                     TADGNTLIDLAWTKHASYNVMIVHRIGSASTVPTQGAAYSVNDACGGGTVIYKGSGAA
                     LEHVVPSAATHHYAFYSYSGNYYSTGVTASASTGAFLPGEIVETFSYTNSTALTGLNG
                     SNLWGGAWYGDTGLFTNSSGSFAEQAVYPAPTGNKLLVTPANDANVAVFRPLGRDYNE
                     GRIYFSYIMNYQCNGPDKYAGLSFFYTNSSEKLFIGEIGSADTTLGIDSTSSSYTLTQ
                     GSGNDYIIVGYYDWGDAEAKVKAYKIGTQSVPSEEPLVWDATVSKASNTVGVINNIRL
                     AAGEFGGGSGTPGNCYFDEVRIATNWFELVQTTPSKIIYDGFGGTDPNSLSGWAGGTG
                     WSDTWALGGDPYVNFSSGSIDDGYSSYYIPTGEKIVMYGNADGRYVTATRTFNQTFTG
                     GQVYFSWMQNYEFNGSGKWAGMKILDSGTEKAFIGKISGADKALGIDDSTHNITSAVV
                     NLENGAGNDYIIVAKYDFDTRELAATSYKVTSDGNCEMVAEEPNGYWQLTTTQSVGHI
                     SSLTGVRLHIGGGMGVQIGDVYMDEVRVGTNWYEVTRQDGETNAAVMAAGPVPTLVYI
                     GTDTVVNGLANLNHDDSDITVSDAELIDETAPLSIAVSWYDPNGVFLTNSTASITNIG
                     SRAGRVNPNWDPALLEGSDLQAVGYDGLFDIFYGDNGDLSVTTGIDNAFSITNDASTS
                     STYYMTVSAEDDNQTGGTLTAPNGGDDVPIWRAITVNSNLTFYVGDDDTTGPTISDFI
                     VGGSGSGGCSGGGLFISEVADPSDTANARFVELFNATGADIDFGSATWYLSRQANGSS
                     WVDHQLTGIITNGDTFVIAYNATFDTKYGFAANNYDGLLITGNGNDGYFLYSGGNHSA
                     GTLVDAYGVVDVDGTSEPWEYEDSHAVRNPDILKGTNVWTASQWTISAAAVANMHPNN
                     HTNNCSGGGASEVTDGQILNGGYGVTVTVADAKSGVLVSNLVANYYLLWNEDSVLACS
                     NFFLTPFTNASLGPVTMVTTGGPAFSSAVVTLGEYTGVVVAADADDDRDGDYTLNTSA
                     AITFTVIDDDTNAPAASDFRIEDSGTTWDSRGDLILTGIVTDVSGVDTGSMYFLMYDE
                     DEVLIWSNSFVISGNADDEYWVSSTSSGPNVDDMCGGIFTINVVAVDGDLDRGVEDTL
                     GTTNFNALTVSVIGEGGAPPAVTNLKVADEEIVDESSLELTDEDINTGGYNLSMILTH
                     EDGLYIGGGTPAFIMYNDFGTEIMNEPFTNIVDVVEGAYTAYYATNLALPGVAAANVD
                     LGQYSVTWSASNQGACIAVEIDSGLITGGTNVFTVVDDDADEPEVDAFAAAGQPAGVS
                     TVSEEDAAAGFSITGLVRDVLSGIDITTAPPTYTIYNPATGTPMGGYENVEFDDLGFG
                     QGEATDYEAIGATSITLPGSCGVIYTVVVSVADADADRATGDQSSLTDQEVLVIQIEG
                     ETGGSLDADGLFVNKDPVEDSNSATVYDDEFVAGSWTMSFAVASTDIEIEGPNKPTFT
                     FYDESEVYVYGGEWETITEAGGKIYATNAPMDPSPNTNFSLGAYRVYWSGRETGLCYA
                     TATESGNFGAAYTNIINVADDDPTYPVIIDNFSVAGGAVTGGVGFIGCDDSRTNLVAG
                     DIAIIGMNTYTKNSPAFVNQDSFAFVATVGIPAGTEIKFTDNGWHCGALYGNEGTITW
                     ISTNCVEPGTVIIYKKYSDPYDSGSSATVNIGQLIDDPMFSVNIRNEEILAYQGDASD
                     PTFLYALDTSITVPGVWEDCPLPTSDKAIRTSLPPGLQNGLTAVCVSNYDDVVLNTNT
                     TAILGDRQTVLEYIGDYHNWIGHDTIVFPLDTWDFTFPELGSVGGTVTDEDILNGTWD
                     ITGQVYDVDSGLSQDSVWYMAFNTSGVAFISAGYFTNAWENPLTATQNLNISTVEEAN
                     SYENVTIGTNNYVTIAAMDVDADRGSDSLTEYLDIPFTVVDDDINPPFFDYLTWNGGT
                     SLDTNQALAGDISVTARVNDIQSGIAFETAPPYILV"
BASE COUNT         2218 a         2989 c         2656 g         2307 t
ORIGIN      
        1 gaccaggata tagggcggcg ccgtttcaaa cgcaataccg ctctggatgt cgttcacacg
       61 cgcggtcacg gaaatatctc cggcgagcgc ctggttggta tccagagatg tcccgccgtt
      121 ccatgtgaga tagtcgaaga acggcggatt gatatcgtca tccacaaccg tgaagggaat
      181 gtccaaatat tccgtgagcg agtcagaacc ccggtccgcg tccacatcca tggcagctat
      241 ggttacatag ttgttcgttc cgatggtgac attttcatag ctgttcgcct cttccaccgt
      301 gctgatgttc agattctgcg tggccgtcag cggattctcc catgcgttgg tgaaatagcc
      361 cgccgagatg aaggccaccc cgctggtgtt gaacgccatg taccagacgg aatcctggct
      421 caaaccgctg tccacatcat acacctgacc agttatgtcc caggtcccgt taagaatgtc
      481 ctcgtccgtc accgtgccgc ccacgctgcc cagttcaggg aacgtgaagt cccatgtatc
      541 cagcgggaac acaatggtgt catggccgat ccagttgtga taatcgccaa tatattcaag
      601 caccgtctgg cgatcgccca gaatggccgt ggtgtttgta ttaagaacca catcgtcata
      661 attgctcaca cataccgccg taagaccgtt ctgcagtccg ggcggcagcg atgttctgat
      721 cgccttatcc gatgtcggga gcggacagtc ttcccacacc ccgggcaccg taatagatgt
      781 gtccagcgca tacagaaagg tcggatcaga agcatccccc tgataggcca gaatttcttc
      841 attcctgata ttgacagaaa acatcgggtc gtcaatcagc tgaccgatgt taaccgttgc
      901 ggaacttcct gaatcatatg gatcactgta cttcttgtat atgattaccg ttccgggctc
      961 cacgcagttc gtggatatcc atgtgatagt accttcgttt ccgtagagtg cgccgcaatg
     1021 ccagccgtta tcagtaaact tgatctcagt gcccgcggga atacccactg tcgccacaaa
     1081 cgcgaaactg tcctgattaa caaacgccgg cgaattcttc gtatacgtat tcataccgat
     1141 gatggcgata tcacccgcca cgaggttcgt gcgcgaatca tcgcagccga tgaagccaac
     1201 cccgccggtc acggcgccgc cggccacgga gaagttgtca atgatcaccg gataagtcgg
     1261 gtcatcatcc gccacattga tgatgttggt gtacgccgcg ccgaagttgc cggactccgt
     1321 ggcggttgca tagcacaatc ccgtctcgcg gccggaccag tagacccggt aggcccccag
     1381 cgagaaattg gtgttgggcg atggatccat cggcgcattc gtggcataga tcttgccgcc
     1441 ggcctctgta atggtctccc actccccgcc atagacatag acttcggact catcgtagaa
     1501 cgtgaaggtg ggcttgttgg gcccttcaat ctcaatgtcg gttgacgcca cggcaaagct
     1561 catggtccag gacccggcca caaactcgtc gtcataaacc gtcgccgaat ttgaatcctc
     1621 caccggatcc ttgttgacga acagtccgtc cgcatccagg ctgcccccgg tttcgccctc
     1681 gatctggata acaagcacct cttgatccgt cagcgacgac tgatcaccgg tagcccggtc
     1741 ggcatccgca tcggcgacgc ttaccaccac cgtgtagatc actccgcaac tgcccggcag
     1801 agtgatgctc gtggcaccaa tggcctcata atcggtagct tcgccctgtc cgaagcccag
     1861 gtcatcaaac tccacattct catagccgcc catcggcgtg cccgtggccg ggttgtagat
     1921 ggtgtaggtc ggcggggctg tggtgatgtc tattccgctc agtacgtcgc gcacaaggcc
     1981 cgtgatggag aatccggcag cggcatcctc ctccgacacg gtcgaaaccc ctgccggctg
     2041 gccggcggcg gcaaacgcat ccacttccgg ctcatcggca tcatcatcca caaccgtgaa
     2101 cacattggtg ccgccggtaa tgagaccact gtctatttcc acagcgatac acgcgccctg
     2161 gttgctggca gaccacgtca cagaatactg tcccagatcc acgttggcgg ctgcaacccc
     2221 cggcagagca agattcgtcg cataatatgc cgtgtaggcc ccctccacca catccacaat
     2281 attggtgaac ggctcgttca tgatctccgt cccaaagtca ttgtacatga taaaggccgg
     2341 tgtgccgccg ccgatataga gtccatcctc atgcgtcaga atcatggaca gattgtatcc
     2401 gcccgtgttg atgtcctcat cggtaagctc cagggaggac tcatcgacaa tttcttcatc
     2461 ggcgactttc aggttggtca cagccggcgg cgcaccgcct tcgccgatga cagaaactgt
     2521 cagcgcgttg aaattggtcg tcccgagggt gtcctcaacg ccgcggtcaa ggtcgccgtc
     2581 caccgccacc acgttgatgg tgaagatgcc gccgcacata tcgtcaacat tcggaccgga
     2641 agatgtgctg gaaacccagt actcgtcgtc cgcatttcca gagataacaa aggagttgct
     2701 ccagatcaga acttcatctt catcatacat caggaagtac atgctcccgg tgtccactcc
     2761 gctcacatcg gtcacaatac cggtcaggat caaatcaccg cgcgaatccc acgttgttcc
     2821 cgaatcctcg atccggaaat cgctggcagc cggcgcgttt gtgtcgtcgt cgatcacggt
     2881 gaaggtgatc gcagcactgg tgttcagtgt atagtcaccg tcacggtcat catcggcatc
     2941 tgccgccacg accacacccg tatattcgcc cagcgtgacc acggcagaac tgaaggcggg
     3001 gccgcccgtg gtaaccatgg tgaccggtcc aagcgacgca ttggtgaacg gggtcaggaa
     3061 gaagttcgaa caagcgagga cgctgtcttc attccacagc aggtagtagt tcgccaccag
     3121 attgctgacc agcaccccgc tcttggcatc ggccacagtc accgtcacac cgtacccgcc
     3181 gttgaggatc tggccgtcag tcacctcaga agcccctccc ccactgcaat tgttggtatg
     3241 attattcgga tgcatgttag ctacagccgc cgccgaaatg gtccattgag aagctgtcca
     3301 gacattggtt cctttgagga tatccggatt tctcacagca tgactgtcct catactccca
     3361 tggctcgctg gtgccatcaa catctacaac tccatatgca tccaccaggg tcccggcaga
     3421 gtgatttcct cccgaataga ggaaatagcc gtcattaccg ttacctgtga tcagcaagcc
     3481 gtcatagttg ttggccgcaa acccgtattt cgtatcaaaa gtcgcattgt aagcgatcac
     3541 aaatgtatct ccatttgtga taataccggt tagttgatgg tcaacccatg agctgccatt
     3601 ggcctgacgg ctcaaatacc aggtcgcgct gccaaaatct atatcggcac ctgttgcatt
     3661 gaacagttcc acgaaccggg cgttcgccgt atcgcttgga tcggccactt ccgaaataaa
     3721 taatccaccg ccagaacaac ctccgctgcc gctgcctcca acaataaaat cgctgatagt
     3781 cgggccggtg gtgtcatcat ccccaacata gaaagtcagg ttggagttaa ccgttatagc
     3841 acgccagatc ggcacatcgt cgccgccgtt tggagcagtc agtgttccgc ctgtctgatt
     3901 gtcatcctca gcactgacgg tcatgtaata ggtcgaactt gtgctggcat cgttcgttat
     3961 actaaaagca ttgtcgatac ccgtcgttac agacaaatct ccgttgtcgc cgtagaagat
     4021 atcaaataaa ccatcgtaac caacggcctg caagtcggag ccctccaaca aagccgggtc
     4081 ccagttggga ttcacgcggc cggcacgcga accaatattg gttatcgacg cggtgctgtt
     4141 cgtcaggaat accccgttgg gatcatacca ggataccgca atggagagcg gagccgtctc
     4201 atcaatcagt tccgcatcac tcactgtaat atcggaatcg tcgtggttca gattggccag
     4261 cccattcaca accgtatccg ttccgatata taccagggtc ggaaccggac cggcggccat
     4321 gaccgccgcg ttggtttcgc cgtcctggcg ggtgacctca taccagttgg tgccgacacg
     4381 aacctcatcc atatacacat caccaatctg cacacccatg ccgccgccaa tgtgcagccg
     4441 cactccggtc agactgctga tatgacccac gctctgagtg gttgtcagct gccagtagcc
     4501 gttcggttct tctgccacca tttcacagtt cccgtcactg gtcaccttgt aggaagtggc
     4561 ggccagttca cgggtgtcaa agtcgtattt tgccactatg atgtagtcat tgccggcgcc
     4621 gttctcgaga ttaacaacag cggaagtgat gttgtgtgtg ctgtcatcaa ttcccagcgc
     4681 cttgtctgcg ccggagatct tcccgataaa ggccttctct gtcccgctgt caagaatctt
     4741 cattcccgcc catttcccgg aaccgttgaa ctcatagttc tgcatccagg agaaatagac
     4801 ctgtccgccc gtgaaagtct gattgaatgt gcgtgtggca gtcacgtacc ggccatccgc
     4861 atttccgtac atcacaatct tttcgccggt cggaatatag taactggagt aaccgtcatc
     4921 aattgaaccg gacgagaaat tcacatacgg atcacctccc agtgcccagg tgtcactcca
     4981 tccggtcccg ccggcccagc cggaaagaga attcggatcc gttccgccga agccgtcgta
     5041 aataatctta gacggggttg tctgcaccag ttcaaaccag ttggtcgcaa tacgcacttc
     5101 gtcgaaatag cagttacccg gcgtgccgga accgcccccg aattcaccgg ccgccagccg
     5161 gatattgttg ataacaccaa ccgtattgct ggccttggag accgtggcat cccagaccag
     5221 cggctcttca gagggaactg actgcgttcc gatcttatag gccttgacct tggcctccgc
     5281 atcaccccag tcatagtagc caacgatgat atagtcatta ccgcttccct gagtcagggt
     5341 ataggaggaa gacgtgctgt caatgcccag cgtcgtgtcg gcgctgccta tttccccgat
     5401 aaagagcttc tctgaactgt tggtatagaa gaacgacagt ccggcatatt tatccgggcc
     5461 gttgcactga tagttcatga tatagctgaa gtaaattctg ccctcgttgt aatcccggcc
     5521 cagaggacgg aacacggcca cgtttgcatc attggccgga gtcaccagca gcttgttccc
     5581 tgtcggagcg ggataaaccg cctgttccgc gaagctgccc gaactgtttg tgaacagtcc
     5641 ggtgtcacca taccatgcgc cgccccacag attgctcccg ttaaggccgg tgagtgcggt
     5701 gctgttcgta taggagaagg tttccacaat ttcaccgggc aggaacgcgc cggtactggc
     5761 gctggcggtc actccggttg aatagtaatt cccgctgtac gagtagaacg catagtgatg
     5821 cgtggccgcc gagggaacca catgctcaag cgccgcgccg gatcccttgt agatcaccgt
     5881 gccgccgccg caggcatcat tcacgctgta ggcagcaccc tgggtcggaa ccgttgaagc
     5941 gctgccgatc cgatgaacga tcatcacatt gtaggaggca tgcttcgtcc aggccagatc
     6001 gatcagcgtg ttgccgtccg ccgtcgctga ggcggcagtc gggtccataa ccggccgtgt
     6061 gtacacagtt tcgacagcac tgaatgatcc tgtgcagtct gccccaatcg aagccacttc
     6121 aaagtagtac ggcgtgttcg gaaccagacc ggtaatggac tggctcgtgc tgctgctgac
     6181 cagcaggttg gagaaacccg gcacccattc tgaggtgcca ctgccgccgc tcacggtatg
     6241 agatccgaga tcggagacgg tatcctgcgc atacccgtcc cactcggtgg ccggatcaaa
     6301 tgcgtcggtg ccgtccgtat cgccttccgt gacagaactc tttctgcgga tggtgttgtc
     6361 cgacgtggat gtatctccag acccccattc agttcccgga tctgttccaa tctgcccgat
     6421 aatatcgcaa taggtattaa gagcagtatt gctgagagcc agcgcatcat ctccattgaa
     6481 agccataaca gaactgttcg tcacgagatc cggcgagccg ccccaggcgg acgcactgtc
     6541 attggctata acatagacat cgccgtctgc cagggtgcct gtcagagtca tggtaaagct
     6601 cggagttggg ctccccccat tgaactgatg aatcacataa tccgacagat ccacatcact
     6661 gcccgtacca ttatatattt cgagatattt gttatacccg cttccctcta tatattctga
     6721 gaagaacagt tcatctgcgg cagcatagtt gctgtataca ttgataatat agtcgctgac
     6781 accgcctacc gccgaccagt ttgccgtata gtcaaacacg tttgtgggat cagcccacag
     6841 ggtgcctggg ccggccggac aactatagac cactgtaaag ggaatattac tgacagaggt
     6901 caaactgtca cctgtccacc cgtcagcatc gttgtcggtc acatatactc tggccgtcca
     6961 cacgccggca ggtgccgaac tgagccctga caatgcgctg tgctgtatct ggccctgaac
     7021 ggttgcagca ccgtcatcaa atttcatgat catctgattg gagtactgca gatctccgtt
     7081 aaaatcatac agcaggaaat aaccagggaa gctgttcgta tccaccccgc tgcccgcatc
     7141 attcaccata ccggtcagcc accatccggt gcccgccagc agcgcgccgc tgccgaccgc
     7201 atccgcgcct tcatcggtac tggacagatg cacgctgtag atattcggat aggcgtcatc
     7261 atcatccgcc gccgtgaagg tgaaggtatc agatccggcg taatcgttca tgtcttccgc
     7321 ccagacaccc actttccagg ttccggccac aaaggatgag gcaatatccg gcaccgtgcc
     7381 cgtggccgtg caggccgttt cacgatcggc atgaccggtg gtcgtgaacg tggtgggtcc
     7441 aacaacctct gaattggacg gattcgttat cgtgaagaaa ggctgcgtgg ccgtggtcca
     7501 gcgcacgccg gtggtggaat cggcgatatc cacgaagatc gggaacccgc tgaaatgccg
     7561 atccagaaca accgtaccgg tcgaatttgt aaccgcaaag ttcgagaacg tgggaccggc
     7621 ggtgtccgcg ctgacaaccg tagtcacctg ttgcgtggac gaccagctgc tttcgcagtt
     7681 ttcattcgtc gccatgacgc gaaaatagta ggttatgccc tgagttagac cggtcacgat
     7741 ctggcttgtg ccggataccg gaaggtcatc aaagccgggg accatgctgc cgccactgcc
     7801 gccagagaaa gtgtgatcat caagatgact ctcatcagtg gcatcgtaca ggtcccattc
     7861 tgtgccaagt gttggaaatc cactgcccgg gtttgatgtc actcctccgg tcactgttga
     7921 ttttctaacc aacgtggcat cagctgtgct gaatgagcca tcggtccact gggctcccgg
     7981 atcctcgcca atgcgcccaa aaatatcaac ccaggaagat gaggtgctct tccaaagagc
     8041 aactgcatca tcgccattat agtttactga agaactgtct gttccaacag tgtttgtaga
     8101 actgctattt ttatagacca ccacatctcc gtccgccagc gttccagaca aagtattgct
     8161 tgctgtatga gatgttgctc cgtttgcata aagaaccagc gcgtaattcg acagatcaac
     8221 actgccccca gtgccattat aaatctcgat gtacttttca ttcccgtact ctatatactc
     8281 agaaataaac agatcagagg cactgccgcc cgcactggaa aaatctgcat tcgtggccac
     8341 atccagaata taaccatccg ccccggtcac agcggaccag gcagcagtaa agtcctgagc
     8401 gttggtctgg ctggcataca ctccggtgac ctcggtaaaa cagtgcactt ccgccctgat
     8461 ggcaaaggtg tatgtgccct catcagtgtc atcgctggcc accgtgacca ggctggtata
     8521 gacaccgccc gggttcgaag gcgccgtgaa gcggatggtg aaggtttcgg cctggccggg
     8581 ggccagtgtc aaatcgccca cattggcctg ccgcgaaaac gccgcatcgc cgctgagcgt
     8641 cggctggccg ctgagcgtca gtgtgtcgtt gccggagttg gtgatggtga agatatggtc
     8701 gtgcgtggtc gaagcttccc ataactggaa gtctgtgccg tccgatacag cggggctgtt
     8761 gtcgccgctg acaatctccg ccagattcgt tcccagcact gcgatttcgg gcgatgtggc
     8821 ggccgatgtc gtggtgacgg aaccggtcga ggacgtggcc gagcagttcg cgttcgtggc
     8881 cgtcacgcgg aagtagtacg tcacgcctcc ggtcagcccc gtcacgctca ggctggtcga
     8941 ggcaacttcc cggttcgtgt agccgggaat gcagttcagt ccgccgccac cgcttccgcc
     9001 actggggaca gatgtacaat catgagaccc cggcgtggat gccccactgc ccgcatcgga
     9061 gactgccgta gccgaccact ctccgcttgc atatgtcgtg acaccagttc caataccggc
     9121 attacgtaca taggttttat tttcgaatgc atctgcagtt gttttcgtat tgtcaattgt
     9181 agttccgctt cttactaacc gtgcgccgtc gccaactttt ccattccatg aggtattgtc
     9241 agaagaccat gaactgtcgg agaaatcagg ggtaaactgc gtattgcttg aatctccgca
     9301 ggtcagcgca ttgctggaac caatggttcc cgaaagtgtc catgtgtgta tgtctgaact
     9361 gttacccacc gccaccacac tccagccggt gagatcaacc gaagcgcttc cagcgttgaa
     9421 aatttcaata taacggtctg atgtgtaatt attctgggga tcgcacatct cagaaataaa
     9481 tatgttgttc gtactcccac cgcccccgcc gccaaagtct tcttcggtgc tgacgtccac
     9541 gacatatccg tcggccccgg agacggcgga ccagttgatg gtgaagttgg tttcgttggt
     9601 gggattgaca taaaccccgg ttaccgagtc gaaacaggtg gtgccgtcgg cggttgaact
     9661 aaaggccgtt tcgacgtttt ctccgttgcg ggccttcact cgaatggagt atgtggtcgc
     9721 ggcggagagc ccggtggccg ttttgggaga tccccatgtg gccttgttct gccagacggc
     9781 cgaggccccc cacgagccgt tcgcctggag atagttgctg tcgctcgtcc gctggatggc
     9841 aaactccgtt gcggcgggat tcgaattctc attgatcgtt atgtccagcg acgaggccgt
     9901 cggattgttc accgtcggcg cgctgggggt gtttgcaagc gtggtgaagc tgagttcaga
     9961 acccagtgtt gtgccggccg tgttgatcgc atacgccttg aaatagtatt tggtgttcac
    10021 ggacagcgaa gaaagattga ccgaaaaagc gcctgttccg ctgccggact gggtcttgtt
    10081 gtccgtgatc gcgaccccgg ccgatgtctt gtagcacaca ccccgattgg tcaccgcggc
    10141 gctgccctga gccgtcacat ttccgcgcgg
//
LOCUS       DAOQWR010000095        10117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7114_length_10117_cov_4.932518, whole genome shotgun sequence.
ACCESSION   DAOQWR010000095 DAOQWR010000000
VERSION     DAOQWR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_10117_cov_4.932518"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1441)
                     /locus_tag="PKK36_08180"
     CDS             complement(104..1441)
                     /locus_tag="PKK36_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNR94571.1"
                     /translation="MASENGRLAVLGCWHQGTVGAACLADFGFDVTGVAASPKDTEQL
                     NKGKAPLYEPGLDDLLLKGVSSGRLRFIDSVAEAVANRPFVLVMHDTPVDENDQSDLN
                     PIFSAVREAAPAMQDGVIVYVTAQAPVGTCDKIAAMIREARPSLKFGIACSPENLRLG
                     QAVQRFIHPALPVLGSDDPAVLDRLEKLFSVFNVKWMRVSLRAAEMTKHALNAFLATC
                     VTFGNELGNICDEVGVDGQAVAKALRLEERVGPKAMLFPGMGFSGGTLARDMQTLRGL
                     GDRYGLETTMLDGVWEANQRQNSFVLRKLKKSLGDLKGRRIAVFGLTYKPQTSTLRRS
                     AALEIIADLKKAGAIITAHDPMADRAEVAQHTEFQFMDDPCAAADGSDALVIITAWED
                     YLKLDYGRILKAMKGRVLIDCSSMLKAEEMDSAGFEYMNVGRGRGGAAQGEKK"
     gene            complement(1456..1797)
                     /gene="yajC"
                     /locus_tag="PKK36_08185"
     CDS             complement(1456..1797)
                     /gene="yajC"
                     /locus_tag="PKK36_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNR94572.1"
                     /translation="MNLTGMFLAMAAPAGAQSQQSPAFNFGWIAIMLAIFYFILIRPQ
                     QKREKERRALLDTVKSGDRIVFGGGILGVVTNVKDKILTVKIADNVKIEVSRAAVSQI
                     LSKGDQPEAAE"
     gene            complement(1811..2950)
                     /gene="tgt"
                     /locus_tag="PKK36_08190"
     CDS             complement(1811..2950)
                     /gene="tgt"
                     /locus_tag="PKK36_08190"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNR94573.1"
                     /translation="MNIGDFKILKKDSRTKARRGRLMTAHGPVETPVFMPVGTQATVK
                     AMSPHEMESLGCRILLGNTYHLNLRPGMDIMEKCGGLHKFMGWNGAILTDSGGYQVFS
                     LTDLRKITKEGVEFQSHVDGARHFLGPREAMRIERILGSDIAMVLDECIPYPCEKNYA
                     AKSVARTIEWAKICRDEPHAEGQLVFGIVQGGIYPDLRERCGQELVKIGFNGYAIGGV
                     SVGEPEDVMIQGVEQGIAALPEDKPRYLMGVGVWTQLVESVARGVDMFDCVMPTRLAR
                     NGTAFTRAGRYPVKAAVYKDDLRPIEEGCTCYACTHFTRAYIRHLLNVNEILGARLLT
                     THNLHCFLRFMEEMREAIEQDRFPEFRERISSEYKTIDKAHAQKK"
     gene            complement(2988..3347)
                     /gene="rsfS"
                     /locus_tag="PKK36_08195"
     CDS             complement(2988..3347)
                     /gene="rsfS"
                     /locus_tag="PKK36_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNR94574.1"
                     /translation="MQIIKALEFMETACEALLSKKGEDLKVLDVRGLSSITDYCVIVT
                     GGSAPQLKALCNELEKAVKTAHGVTVSFRAGSPESGWMLVDYFDAVFHVFSREKREYY
                     DLERLWSDAPRLKKFEG"
     gene            complement(3350..3973)
                     /gene="nadD"
                     /locus_tag="PKK36_08200"
     CDS             complement(3350..3973)
                     /gene="nadD"
                     /locus_tag="PKK36_08200"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNR94575.1"
                     /translation="MNGEKHKIGLLGGSFNPVHMGHLILAQDAMERAELDEMIFIPCA
                     TPAHKDPGSLISFEHRFRMLELAVEDNPDFSVSNVEKRRDGISYTIDTIRLLKQSHPD
                     ADFSFIIGSDSLPELQTWMKIYELLEECRFVIMARPGFRAAEMTRERINLRDPWPERL
                     QKQVITGHEIEISSTDVRMRVAEGMSIRYLVPPPVEMYICEHNLFQT"
     gene            complement(4117..4434)
                     /locus_tag="PKK36_08205"
     CDS             complement(4117..4434)
                     /locus_tag="PKK36_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNR94576.1"
                     /translation="MGNPDKKSAFKERVGYWADKLDVKVVWLGVRPMRSKWASCSTSG
                     HLNFNSELIGLKSDLWDYVIVHELLHFFVPNHGKLWKSLMRVHLGDYERLDAELRRGI
                     ARA"
     gene            complement(4438..7356)
                     /locus_tag="PKK36_08210"
     CDS             complement(4438..7356)
                     /locus_tag="PKK36_08210"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNR94577.1"
                     /translation="MKPGEHKTVQAPILKYAQEIGWTFVPREEAEARRGFDPEADSGE
                     RAKNGSMFFDDLLDARVREFNPRYAEAEGALPGQFRHLHADIYGNRDFVEYLRNRGKF
                     FDHEEKRERDLVLINYDDPAKNIYEVTEEWAFHNGHCGTREDVVFLINGIPVLVIECK
                     NAGRDEGIALGIDQIRRYHRETPELFVPEQIFTATDVIGFSYGATWNTVRRNIFNWKS
                     EEVGKLESKIKSFCAIPQVLAFLKDYIVFAEKDEELNKYILRQHQTGAVENVIQRSLD
                     SKRRRGLVWHTQGSGKTFTMIKAAEKLFRAPEADKPTVLLMIDRNELEDQMLKNLAAL
                     GLGNLEHAGSIARLNRLLKDDYRGIIVTMIHKFRDMPADINTRENIYVLIDEAHRTTG
                     GDLGNFLMAGLPNATFIGFTGTPVDKTVYGRGTFKTFGCEDDKGYLHKYSIADSIEDG
                     TTLPLYYQLAPSEMLVPHETLDKEFLSLAEAEGVADIEELNKILERAVNLKNFLKGRD
                     RIHKVAQFVAKHYRENVEPLGYKAFLVGVDREACARYKHALDQFLPPEYSEVVYTGSN
                     NDSALLKEFHLDPKKERQIRKSFGKIDQQPKILIVTEKLLTGFDAPVLYAMYLDKPMR
                     DHTLLQAIARVNRPYENETQEMVKPHGFVLDFVGIFDKLEKALAFDSDEINAIVKDLK
                     LLKVLFKNKMESKAPEYLDLIKRNFNDKDVDILIEHFRDPERRKEFFKEYKEIEMLYE
                     IISPDAFLRPFIDDYATLSAIFNVVRKSYARTVPVDRDFQRKTNELVQKHIGTTYAQP
                     VTEALKINEDTVRYIAANKGGDNSKVINLIKSIEKAAEEQKNDPYLIAMAERARIVQE
                     AFEGRQATTAETLARLLREVEANETRKTEQAAKGFDGLTFFIYRMLEDAQVSAAETVS
                     RKIRDAFIEMPNWKRSENELRELRKKVTFAIFAQCDDLDKVTALVNSMFRVLEKADRI
                     "
     gene            complement(7353..8660)
                     /locus_tag="PKK36_08215"
     CDS             complement(7353..8660)
                     /locus_tag="PKK36_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNR94578.1"
                     /translation="MSTVAEVRLWGRTISAVALEDGADCASFEYDGAFAQSGIELSPL
                     CMPLSRRIYRFPELSRQTFHGLPGLLADSVPDRFGNALIDAWLATQGRTPESFNAVER
                     LCYTGSRGTGALEYLPAIGPAARKSSRIRVAKLVELASEILTHRSSLQVSFHDKSRET
                     ALKDILRVGTSAGGARAKAVIAWNPQTNAVRSGQVAAGSGFEYWLMKFDGVSGNQDKE
                     LEDPKGYGAIEFAYSKMAKEADIVMSPCRLFEENGRRHFMTKRFDRLDGGDKLHMLSL
                     GALAHFDMNLAGAYGYEQALQAVKKLDLGMEAVEQLYRRMVFNVLARNQDDHVKNIAF
                     LMDREGRWSLAPAFDMTYSYNPSGRWTSSHQMTINGKRDGFTLDDFKACAEAASMKRG
                     REKMIISEVHKAVSRWRDLADEAGVDSEQRDKIQKTLRLESFK"
     gene            complement(8657..9004)
                     /locus_tag="PKK36_08220"
     CDS             complement(8657..9004)
                     /locus_tag="PKK36_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR94579.1"
                     /translation="MKIDALQTDDAVLAELGQRLARRRIDMGLTQEELAAEAGVAKRT
                     LERMEAGSTAQMKTVLRVFRVLDLMPRLESLLPERGPSPMELLKQKGKARQRASSGMV
                     RESAEPWHWGDKK"
     gene            complement(9524..>10117)
                     /locus_tag="PKK36_08225"
     CDS             complement(9524..>10117)
                     /locus_tag="PKK36_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94580.1"
                     /translation="DDNFGPNFCIPRLYVRPEQVQYVVELKRGNAQYGGIVAEAQALQ
                     FLYSLNPQLNLQNVWLKRLQYCSLPHVQQVVLRAICISREEYISHLESLTDWDNQTES
                     ANIIDVLIKYLPELLWVVEVSIPHLFPANEHKLGEILLNALQARDSKKSIDYKLFMLA
                     RFPAKYFLLDSVNQHGPSFLMIPSQLISHTPVFRTTQ"
BASE COUNT         2122 a         3011 c         2626 g         2358 t
ORIGIN      
        1 ccggcatgat cgctggcgat caccgccagt tctgtttccg gaaaatcatt gatgacgggc
       61 gcgcgcctgc ggcactgcag tcctgcgccg ataatcgcaa ctttcatttc ttttctccct
      121 gcgccgcgcc tccgcggccg cggccgacat tcatatactc gaaacccgcc gaatccattt
      181 cttccgcttt gagcatgctg ctgcagtcta tcagaacgcg gcccttcatc gctttcagga
      241 tgcgcccgta gtccagcttg agataatcct cccatgccgt gatgatcacc agcgcatcgg
      301 agccgtcggc cgcggcgcag ggatcatcca tgaactggaa ctcggtgtgt tgcgccacct
      361 ccgcgcggtc cgccatggga tcgtgcgccg tgataatcgc gcccgccttt ttcagatcgg
      421 ctatgatttc cagtgcggcc gaccgtcgca gcgtgctggt ctggggtttg taggtcaggc
      481 cgaacacggc gatgcggcgg cctttcagat cgccaaggct tttcttcagc ttgcgaagca
      541 caaaactgtt ctgccgctgg ttggcttccc agacgccgtc cagcatcgtg gtctccagcc
      601 cgtaccggtc gcccaatccc cgcagtgtct gcatatcgcg ggccagtgtg cccccggaga
      661 agcccatgcc cggaaagagc atcgctttcg ggcccacgcg ctcctcaaga cgcagggcct
      721 tcgccacggc ctggccgtcc acacccactt catcacagat gttgccgagt tcgttgccaa
      781 acgtgacaca ggtggccagg aaggcgttca gcgcatgctt ggtcatctcc gccgccctga
      841 gtgaaacgcg catccatttg acgttgaaaa cagaaaacag tttttcgagc cggtccagca
      901 cggccggatc gtccgaaccg agcaccggca gggccggatg gataaagcgc tgcacggcct
      961 gacccagccg cagattttcc gggctgcagg cgattccgaa tttcagcgac ggcctggcct
     1021 cgcgaatcat ggcagcgatt ttgtcgcagg ttccaaccgg cgcctgagcc gtaacataca
     1081 caatcacgcc gtcctgcatg gccggcgccg cttcgcggac tgcgctgaaa atggggttca
     1141 ggtcgctctg gtcgttttca tccaccggag tgtcgtgcat gacaaggaca aagggcctgt
     1201 ttgcaacggc ctcggcgacg gagtcaatga agcggaggcg gcccgaggaa acgcctttca
     1261 ggagaagatc gtccagcccc ggctcgtaga gcggggcttt gcctttgttc agctgctccg
     1321 tgtccttcgg actggcggca acgccggtca cgtcgaaccc gaaatcggcc aggcaggccg
     1381 cgccgaccgt gccctggtgc cagcatccga gaactgccag tctgccattt tcagaggcca
     1441 tacccgcgca aacccttatt ccgcggcttc gggctgatcc cccttgctga ggatctgcga
     1501 caccgcggcc cggctgactt cgatcttcac gttgtctgcg attttcaccg tcagaatctt
     1561 gtccttcaca ttcgtgacca cgccgagaat gcctccgccg aacacaatgc ggtcgccgct
     1621 cttcacggtg tccagcaggg cccggcgctc tttttcgcgc ttctgctgcg ggcggatgag
     1681 gatgaaatag aaaatcgcca gcatgatggc gatccagccg aaattgaagg ccggggactg
     1741 ctggctctgc gcgcccgcgg gggcggccat ggctaaaaac atgcctgtga gattcatgaa
     1801 accattctcc ttattttttc tgcgcgtgcg ctttgtctat cgttttgtat tcgctgctga
     1861 tcctctccct gaactcgggg aaccggtcct gctcaatcgc ctcccgcatt tcctccatga
     1921 accggaggaa gcagtgcagg ttatgggtgg tcagcagccg ggcacccagg atctcgttca
     1981 cattcagcag atggcgaata taagccctgg tgaaatgagt gcaagcataa catgtgcacc
     2041 cctcctcgat cgggcgaagg tcatccttgt aaacggcggc cttgacggga tagcggcccg
     2101 cgcgcgtgaa ggccgttccg ttgcgcgcga ggcgggtggg catgacgcag tcgaacatgt
     2161 ccacgccgcg ggcgacggac tccacgagct gggtccagac gcccacgccc atcagatagc
     2221 ggggcttgtc ctcgggcagg gccgcaatcc cctgctccac gccctggatc atcacatctt
     2281 ccggctcgcc gacgctgacg ccgccgatgg catagccgtt gaagccgatc ttcaccagct
     2341 cctgtccgca gcgctcgcgc aggtcgggat aaattccgcc ctgcacgatg ccgaacacga
     2401 gctggccttc cgcatgcggc tcatcgcggc agattttcgc ccattcaatc gtgcgggcaa
     2461 ctgattttgc ggcgtagttc ttttcgcagg gatacgggat gcactcatcc agcaccattg
     2521 cgatgtcgga acccagaatg cgctcaatgc gcatggcctc gcgcggaccc aggaagtgac
     2581 gggcgccgtc cacatgcgac tggaattcca cgccttcttt cgtgattttg cggagatcgg
     2641 tgaggctgaa cacctgatag ccgccgctgt ccgtcaggat ggccccgttc cagcccatga
     2701 acttgtggag gccgccgcat ttttccatga tgtccatgcc gggccggagg ttcaggtgat
     2761 aggtgttgcc cagaaggatt ctgcacccca gggattccat ttcgtggggc gacatggcct
     2821 tgacggtggc ctgtgtgccc accggcatga aaacgggggt ttccaccggg ccgtgcgccg
     2881 tcatcaggcg tccgcggcgg gcctttgtcc ggctgtcttt tttaagaatt ttgaaatcgc
     2941 cgatgttcat gtcatgcgcg catttgcgga atgcctttgg ggaggagtca gccttcaaac
     3001 ttcttcaggc gcggcgcatc gctccagagc cgctccagat cgtagtattc gcgcttctcc
     3061 cggctgaaca catggaacac tgcgtcgaaa tagtccacca gcatccagcc gctttcggga
     3121 cttcccgcgc ggaaactgac ggtgacgccg tgagcggttt tcaccgcctt ttccagttcg
     3181 ttgcagagcg ccttgagctg cggggcgctg ccgccggtca caatcacgca gtaatctgta
     3241 atgctggaaa gcccccggac atccagcacc ttcagatctt cacctttttt ggagagcagc
     3301 gcctcgcagg ccgtctccat gaattccaat gccttaataa tctgcactcc tatgtttgaa
     3361 acaaattgtg ctcgcaaata tacatttcca ccggcggcgg cacaagataa cgaatgctca
     3421 tgccctccgc cacccgcatc cgcacatcgg tcgaggatat ctcgatctca tggccggtga
     3481 tcacctgctt ctgcagccgc tccggccagg gatcgcgcag attgatccgt tccctggtca
     3541 tctcggccgc ccggaatccg ggccgggcca tgatcacaaa ccggcattcc tccagcagct
     3601 cgtagatctt catccacgtc tgcagttccg gaagcgaatc ggacccgatg atgaaactga
     3661 aatcggcatc cggatgactc tgtttcagca gccggatggt gtcgattgtg taggagatcc
     3721 cgtcgcgccg tttctcgaca ttcgacacgc tgaaatcggg attatcctcc accgccagtt
     3781 ccagcatgcg gaagcggtgc tcaaaactga tcagcgaccc gggatccttg tgggcgggcg
     3841 tggcgcacgg gatgaaaatc atctcgtcca gttccgcccg ctccatcgca tcctgcgcaa
     3901 ggatcagatg ccccatatgg acgggattaa atgatccccc cagcagacct attttgtgtt
     3961 tttctccatt catggttccc tatgtattta aacatcagaa acggccataa cacaaagcta
     4021 atatatttcg ctgcttgtgc tgcagttaac gcaaacgccg ggaaactgca caaacagggg
     4081 gccaaacagt gtcttgtatg cttcagcacg gcggcattaa gcccgagcaa tccctcggcg
     4141 caattccgca tcgagccgct catagtcgcc cagatggaca cgcatgaggc ttttccagag
     4201 tttgccgtga ttaggaacga aaaaatgaag cagttcatga acgatgacat aatcccagag
     4261 gtccgatttc agcccgatca gttcactgtt gaaattcaaa tgtccgctgg tggagcatga
     4321 ggcccatttg ctgcgcatcg gccgcacccc cagccagaca accttcacgt cgagtttatc
     4381 agcccaatag ccgacccgct ccttgaacgc tgatttttta tctggatttc ccatacatca
     4441 aatccggtca gccttttcca ggacacgaaa catgctgttc acgagcgcgg tgactttatc
     4501 cagatcatcg cactgcgcaa agatggcaaa agtgactttt ttgcgcagtt cccgaagctc
     4561 gttttcgctt cgtttccaat tcggcatttc gataaaggcg tcccgaatct tccggctgac
     4621 agtctcggcc gcgctgacct gtgcgtcctc cagcatgcgg tagatgaaga aggtcagccc
     4681 gtcaaaaccc tttgcggcct gctccgtttt gcgcgtttca ttcgcctcca cctctcggag
     4741 cagtctggcc agcgtctccg ccgtcgtggc ctgccggccc tcaaaagcct cctgaacaat
     4801 acgggcgcgc tcagccatgg caatcagata gggatcgttt ttttgctctt ccgcggcttt
     4861 ctcgatgctt tttatcaggt tgataacctt gctgttgtcg cctcctttat tggcggcgat
     4921 atacctgacg gtatcttcat tgatcttgag agcttctgtg acgggctggg cataagtggt
     4981 gccgatatgc ttctgaacca gttcatttgt cttgcgctgg aaatcgcggt caactggaac
     5041 tgtccgggca taggacttgc gaacaacgtt gaagatggcc gacaacgttg cgtagtcgtc
     5101 aatgaatggc ctcaagaagg catcgggcga gatgatctca tagagcatct ctatctcctt
     5161 gtattccttg aagaactcct tgcggcgctc cggatcgcgg aaatgctcaa tgagaatatc
     5221 cacgtccttg tcgttgaaat tgcgtttgat caggtcgaga tattcgggag ctttggactc
     5281 catcttgttt ttgaaaagca ccttcaggag cttcagatcc ttgacaatgg cattaatttc
     5341 atcgctgtcg aaggcaaggg ccttttcgag cttgtcaaag attccgacaa aatcgaggac
     5401 aaagccgtgg ggcttcacca tctcctgcgt ttcgttctca tagggccggt tcactcgcgc
     5461 gatcgcctga agcagggtgt ggtcgcgcat cggcttgtcg agatacatgg cataaagcac
     5521 gggggcatcg aagccggtga gaagtttttc tgtaacgatc agaatcttcg gctgctggtc
     5581 aatctttccg aagcttttgc ggatctgccg ctccttcttg gggtcgagat ggaattcctt
     5641 cagcagggcg gaatcgttat tgctgccggt gtagacgacc tcggaatact cgggcggcag
     5701 gaactgatca agggcgtgtt tgtagcgggc gcaggcttcg cggtccacgc cgacgagaaa
     5761 ggccttgtaa cccagcggtt cgacattctc ccgatagtgc ttggcgacga actgtgccac
     5821 cttgtgaatc cggtcccggc ccttgaggaa attcttcagg ttcaccgcac gctctagaat
     5881 cttgttcagc tcttcaatat cggccacccc ctcggcttcg gcgagggaga gaaactcctt
     5941 gtccagcgtc tcatgcggaa cgagcatttc gctgggcgca agctggtaat acagcggaag
     6001 cgtcgtgccg tcctcgatgc tgtcggcgat ggaatacttg tgcaggtagc ccttgtcgtc
     6061 ctcgcatcca aaggttttga aggtgcccct gccgtagacg gtcttatcca ccggcgtgcc
     6121 ggtgaatccg atgaatgtgg cattcggcag gccggccatc aggaaattgc caagatcgcc
     6181 gccggttgtg cggtgggctt cgtcaatgag cacgtaaata ttctcgcggg tattaatatc
     6241 cgccggcata tcgcggaatt tgtggatcat cgtcacaatg atgccccgat agtcgtcctt
     6301 cagaagccgg ttgagccggg cgatgctgcc ggcgtgctcc agattgccca gaccgagcgc
     6361 agcgagattc ttgagcatct gatcttcgag ttcattgcgg tcaatcatca gaaggacggt
     6421 gggcttgtcg gcctcgggag cgcgaaaaag cttttctgcc gccttgatca tcgtgaaagt
     6481 cttgccgctg ccctgcgtat gccacaccag tccgcgcctg cgttttgaat ccagcgagcg
     6541 ctgaatcaca ttctccacag cccccgtctg atgctgacgc agaatgtact tgttcagctc
     6601 ctcatccttt tcggcgaaga caatgtaatc cttcagaaaa gccagcacct gcggaatggc
     6661 gcagaaactt ttgattttcg actccagctt cccaacctct tcggacttcc agttgaagat
     6721 gttccggcgc accgtgttcc aggttgcccc gtaagaaaat ccgatcacat ccgttgccgt
     6781 gaaaatctgt tccgggacaa acagctcggg cgtctcgcgg tgataccgcc ggatctggtc
     6841 aatacccagc gcaatgccct catccctgcc ggcgttcttg cactcgatca ccagcaccgg
     6901 aatgccgttg atcaggaaga cgacatcctc ccgcgtgccg cagtgaccgt tgtgaaaggc
     6961 ccactcctca gtgacttcgt aaatattttt cgcggggtcg tcgtaattga tcagaaccag
     7021 gtcgcgctcg cgcttttcct cgtgatcgaa gaacttgccc cggttgcgca ggtattccac
     7081 aaagtcgcgg ttgccgtaaa tgtccgcatg caggtgccgg aactgacccg gcagcgcccc
     7141 ttcggcctcc gcatatcgcg gattgaattc ccgcaccctg gcatccagca ggtcatcgaa
     7201 gaacattgat ccattctttg cccgctcgcc cgagtctgcc tcgggatcaa accctctccg
     7261 agcctccgcc tcctcgcgcg gaacaaacgt ccagccgatc tcctgagcat acttcagaat
     7321 cggcgcctga acggtcttat gttcgccggg tttcatttga aagattccaa acggagggtt
     7381 ttctggattt tgtcgcgctg ttcagaatcc accccggcct cgtccgcaag atctctccag
     7441 cgagacaccg ccttatgcac ctctgagata atcatcttct cccgtccgcg cttcatggat
     7501 gccgcctccg cgcatgcctt gaaatcatcc agcgtaaacc cgtcacgctt accgtttata
     7561 gtcatctgat gactggatgt ccatcttcct gacggattat agctgtaggt catgtcaaag
     7621 gcgggtgcca gggaccagcg cccctcacga tccatgagaa aagcaatatt cttcacatga
     7681 tcatcctgat tgcgcgcaag cacattgaaa accatgcgcc ggtagagctg ctccacggcc
     7741 tccattccca gatccagttt tttgaccgcc tgcagagcct gctcatagcc gtaagccccc
     7801 gccagattca tgtcaaaatg agccagcgct cccagtgaaa gcatgtgcag cttgtccccg
     7861 ccatccagac ggtcaaaacg cttcgtcata aaatggcggc ggccgttttc ctcaaagagt
     7921 ctgcagggac tcatgacaat atccgcttcc ttggccattt ttgaataggc gaattcaatt
     7981 gcgccgtagc ctttcggatc ctccagctcc ttgtcctgat tcccgctcac accgtcaaac
     8041 ttcatcagcc agtattcaaa acccgatccg gcggcgacct gccccgagcg caccgcattg
     8101 gtttgcggat tccaggcaat caccgccttc gcgcgtgccc cgcccgccga tgtgccgacc
     8161 cgcagaatat ccttcaatgc cgtttctctg ctcttatcgt gaaacgaaac ctgcaggctg
     8221 ctgcgatggg ttagaatttc tgatgccagc tctactagct ttgcaacccg aatgcgggag
     8281 gacttccggg ctgcaggccc tatggctggt aaatattcaa gcgcacccgt gccgcgcgat
     8341 cccgtgtagc acaaacgctc aaccgcattg aacgactcgg gcgtgcggcc ctgcgtggcc
     8401 agccaggcat caatcagcgc attgccgaac cggtccggga cagaatccgc cagcagtccg
     8461 ggcaggccgt gaaaagtttg tcgcgaaagt tcgggaaacc ggtatattct ccgggacagc
     8521 ggcatgcaaa gcggagagag ctcaataccg ctctgtgcaa acgcgccatc gtactcaaac
     8581 gatgcacaat cagccccatc ctcgagcgcg accgcgctga tcgttcttcc ccacaaacgg
     8641 acctctgcga ccgtgctcat ttcttatcgc cccagtgcca gggttctgcg gactccctga
     8701 ccatgccgga agatgcgcgc tgacgcgctt tccccttctg cttgagaagc tccatcggac
     8761 tgggtccccg ctcgggaagc aggctttcca gacgcggcat cagatccagc acgcggaaaa
     8821 cgcgcagcac cgttttcatc tgggcggtgg aaccggcctc catgcgttca agcgtgcgtt
     8881 tggccacacc ggcctccgcg gcaagctcct cctgagtcag acccatatca atacgccgcc
     8941 gggcaaggcg ctgtcccagt tctgccagaa ccgcatcatc cgtctgcaac gcatcaatct
     9001 tcatagcatc cacctttcta aaagcaccta tatcgcaata gatggcgaat agcaagcata
     9061 tttggattta tttcgcaata tttgacgact tgccaaacaa aataagcatc cccttgcaag
     9121 aaacccgctt cagactaaac agcttcagac tggtcggccc tgtgggacga caatgtttat
     9181 ttgacgcaaa agaagaattt ccaccatgaa ggacttgaag atcatgaaga tgaatagttt
     9241 tcctgaatta tttgcccctt taatccgtgg tttcttcttc atgctcttcg tatcttcatg
     9301 gtgatctctg ttttggctgc ggccctgccg catgggatat ccgtggcaaa gaatcttcaa
     9361 aaatcaccaa aaacagccaa aaaacgttaa aaaacgctca aaaatgccat tttttgaaca
     9421 tttttcgcca tttttgacga tttaaggcca tttttcagca tatttcgcca tttctgacga
     9481 cttcttttaa ttggcaagaa cacgttgaga ctcaaccaat accctattga gttgttctaa
     9541 acacaggtgt atgggatatc agttggctgg ggatcatcag gaaactgggg ccatgctgat
     9601 taacggaatc caaaaggaag tacttggccg gaaatctcgc caacatgaat agcttataat
     9661 ctatactctt tttgctgtct ctcgcctgta atgcattcag caggatttca ccgagcttat
     9721 gttcatttgc ggggaacaaa tgtggaatag atacctcaac cacccacaaa agctcgggca
     9781 agtacttgat aagaacatca atgatatttg cgctttctgt ctgattgtcc cagtcagtca
     9841 aactttccag atgactaata tattcttctc tgctgatgca aatcgcgcgc aagacaactt
     9901 gttggacatg aggaagagaa cagtactgca accttttcaa ccaaacgttt tgcaggttta
     9961 gctggggatt cagagaatac aagaattgaa gagcctgcgc ttccgcaaca atgcccccat
    10021 attgagcatt tccccttttt aactcaacga catattgtac ttgctcgggt ctgacataca
    10081 gccggggaat gcagaaattt gggccgaaat tatcatc
//
LOCUS       DAOQWR010000096        10051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7176_length_10051_cov_4.878551, whole genome shotgun sequence.
ACCESSION   DAOQWR010000096 DAOQWR010000000
VERSION     DAOQWR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_10051_cov_4.878551"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3223)
                     /gene="rpoB"
                     /locus_tag="PKK36_08230"
     CDS             complement(<1..3223)
                     /gene="rpoB"
                     /locus_tag="PKK36_08230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR94581.1"
                     /translation="MVDRIKFGKLADVISLPDLIEIQTSSYKDFLQMDVAPTRRRRVG
                     LQAVFKEVFPIESYDGQCVLDFVKYEIGEPKMSEVECLREAETFAAPMHVTFRLKEGK
                     SVQEEVVYMGEVPLISSSGSFIVNGAERVIVSQLHRSPGICFEQSVHANGSLLYSFRI
                     IPDRGSWVEVQFDTSDLLYIYLERKRRRRKFLATTFLRALGFGSDEELIRLFYTEETL
                     KLSRDFTEEELANKVLKDDIVDADSQSILARRYDALTPETVKAMKAAGYKTADIVNVS
                     WDEGIFMKSIQKDPAHSMDEALKDIYHKLRPGDPPTVSNARQLIRRIFFDPRRYDLGR
                     VGRYKIQQKLGQEPNDSLVLRTEDLVDAVRYLINLRRGEGTIDDIDHLGSRRVRTIGE
                     LLEGQCRVGLARTERLVKERMTLYDAATDKLTPQRLINPKALSAVIKDFFGRSQLSQF
                     MDQTNPLAELTHKRRLSALGPGGLSRERAGFEVRDVHSSHYGRICPIETPEGPNIGLI
                     SSLSCYARVNEFGFIESPYRKVINGRVSDEVYYLTADQEEKYVIAQVNAPLTAKGTFE
                     RELVSVRYRGDFLEVPRERVDYMDVSPKQLVSVAASLIPFLEHDDANRALMGSNMQRQ
                     AVPLLVPERPYVATGMEGPVARDSHVMVLAERPGKVAYVSADRIIVSKNGEMPTKKTP
                     AAEAQVYELRKFMRSNAATCINQKPCVAQGDTVKAGDVLANGPGTSHGELALGRNVLV
                     AFMPWCGYNFEDAILISEKLVREDVYTSVHIEDFECGARDTKLGPEEITRDIPNVGED
                     ALANLEHDGVIRIGAEVKPGDILVGKITPKSETELAPEERLLRAIFGEKAADVRDTSL
                     RVPSGTYGIVMDVKVSARNPVQQTKMTPTAIRRQKKAIEEEFADRSKQLTEDLTEALS
                     NILLGEKIPLDVVNIETGEIIIPANRKITKTLLRKLAAAHNNIEIDPSPIQIKIQGII
                     GEYSGKFADLQEEQERAMDRLDSGDEIDQGIIKQVRVYIASKKKLSVGDKMAGRHGNK
                     GVISRIVPEADMPFLPDGTPVEIVLNPLGVPSRMNVGQVLETHL"
     gene            complement(3313..3729)
                     /gene="rplL"
                     /locus_tag="PKK36_08235"
     CDS             complement(3313..3729)
                     /gene="rplL"
                     /locus_tag="PKK36_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNR94582.1"
                     /translation="MTDKVEEKTAPVLEGKAAEFVDWIEGISVLELSKLVKALEVRLG
                     VSAAAPVAMAAAPAAGGGAAAAEEKTEFAVVLASAGAQKIQVIKEVRAITGLGLKEAK
                     DLVDGAPKTVKEGVDKAEADAMKKQLEAAGATVEIK"
     gene            complement(3757..4278)
                     /gene="rplJ"
                     /locus_tag="PKK36_08240"
     CDS             complement(3757..4278)
                     /gene="rplJ"
                     /locus_tag="PKK36_08240"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR94583.1"
                     /translation="MRPEKKSIVDEMRAEAKDAGFVILTDYRGLSVAQTENLRTQLAR
                     VDARFRVIHNRMFKKFTGELSLDGFDSALKGPSAVVTGRGDVAETARVLKDFMRANKM
                     PSVKMAAVEGQLLKADEVDELAELPAKPVMQAILLGTLAAPMTRLAGVLSQKVASVLY
                     VLKAVEEKKQTSL"
     gene            complement(4291..4992)
                     /gene="rplA"
                     /locus_tag="PKK36_08245"
     CDS             complement(4291..4992)
                     /gene="rplA"
                     /locus_tag="PKK36_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNR94584.1"
                     /translation="MATHGKKYTDVRTALNRSEVYSIDAAIEVLKKSSTAKFDETAEV
                     AFRLGVDPKKSDQMVRGTVSLPHGSGKTVRVVVFATGPAADAAREAGAAEVGMDDLIA
                     KVTGGWTDFDIAVATPEAMKEVRKLGKVLGPRGLMPNPKTGTVSDDTATAVKQFQAGR
                     VEFKMDRHGNVMVPFGKLSFTAEQLAENCRAVIEAVVAARPAVAKGIFLKKCVVTSTM
                     GPGLQINVKDYAGAA"
     gene            complement(4995..5417)
                     /gene="rplK"
                     /locus_tag="PKK36_08250"
     CDS             complement(4995..5417)
                     /gene="rplK"
                     /locus_tag="PKK36_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR94585.1"
                     /translation="MAKKVTGIIKLQIPAGQANPAPPVGPALGQQGVNIMEFCKAFNA
                     ATQKQAGMVIPVVITVYQDKSFTFITKSPPAATLLKKAAGIAKGSAMPNRDKVGKVTK
                     AMVREIAEKKKDDLNARDIEQAMRMVEGTARSMGIEVE"
     gene            complement(5435..5983)
                     /gene="nusG"
                     /locus_tag="PKK36_08255"
     CDS             complement(5435..5983)
                     /gene="nusG"
                     /locus_tag="PKK36_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR94586.1"
                     /translation="MDKQWFVLHTLSGQENKVKENIERRTVLEEMGEYIGEVLIPTEK
                     VSEVKQGKKMTTTRKFYPGYVLINLALYDADHKINEKTWYFIQETQGIIGFIGGDNPV
                     PLRPAEVDVILNQIEEKKEKVKPKVNFEPGEPVKVTDGPFLNFSGVVEEVDPDHGKLK
                     ISVSIFGRTAPVELEYWQVERE"
     gene            complement(6011..6214)
                     /gene="secE"
                     /locus_tag="PKK36_08260"
     CDS             complement(6011..6214)
                     /gene="secE"
                     /locus_tag="PKK36_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR94587.1"
                     /translation="MNRLSAGVSGFRNFMGEVWQELRKCNWPTRPELMESTVIVIVSV
                     LLVGIYVSICDVVAQGFLNLVIR"
     gene            complement(6236..6311)
                     /locus_tag="PKK36_08265"
     tRNA            complement(6236..6311)
                     /locus_tag="PKK36_08265"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6276..6278),aa:Trp,seq:cca)
     gene            complement(6328..6480)
                     /gene="rpmG"
                     /locus_tag="PKK36_08270"
     CDS             complement(6328..6480)
                     /gene="rpmG"
                     /locus_tag="PKK36_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNR94588.1"
                     /translation="MAREIITLACTECKRRNYTTTKDKRLTPGRMEKKKYCPFERKHI
                     LHRETK"
     gene            complement(6567..7769)
                     /gene="tuf"
                     /locus_tag="PKK36_08275"
     CDS             complement(6567..7769)
                     /gene="tuf"
                     /locus_tag="PKK36_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNR94589.1"
                     /translation="MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITKVQEAKGLATF
                     ISYDQVAKASESQGRRDSTKILTIATSHVEYQSANRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSASDGPMPQTREHILLARQVGVPSIVVFLNKVDTVDDPELLDLVELEIR
                     ELLNKYEFPGDDTPIIRGSALKATENPSGPDAECIQKLMDALDAWVKEPPRPLDQPFL
                     MPIEDVFSIEGRGTVVTGRIERGIIKVGEEVAIVGIRDTTKTTVTGVEMFRKLLDQGQ
                     AGDNVGCLLRGVKKDDVERGQVLAKPGSITPHTKFTGEVYVLSKDEGGRHTPFFKGYR
                     PQFYFRTTDVTGDIILPEGVEMVMPGDNVSMEVTLLTPIAMEEHMRFAIREGGRTVGA
                     GRVSKILE"
     gene            complement(7845..7920)
                     /locus_tag="PKK36_08280"
     tRNA            complement(7845..7920)
                     /locus_tag="PKK36_08280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7885..7887),aa:Thr,seq:ggt)
     gene            complement(8189..8557)
                     /locus_tag="PKK36_08285"
     CDS             complement(8189..8557)
                     /locus_tag="PKK36_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94590.1"
                     /translation="MPKVLLVDDDAAIIETLQCLVSVRGGEVVALTDSQEAADRIQAG
                     EQFDLMLTDLRMKPVDGIQLITLAREMLPDMPVIVVSAYLDDKTIEHIQELGCRAYLD
                     KPFSVDDVNSLLQMYLGPSA"
     gene            complement(8625..>10051)
                     /locus_tag="PKK36_08290"
     CDS             complement(8625..>10051)
                     /locus_tag="PKK36_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNR94591.1"
                     /translation="LLAAGALSLIPAYKAFSVYSYNSTAKQLELQVSTLLNASPLTVD
                     WSPAGDFLAVGTELNSTGPELYLYEYNRLSGALRLLNSNEHISAVNSVRWSSDGKYLA
                     VGDSQNKLTVYRMLFADLAVKKTASTNVVMDGQQFEYHLYVTNRGPDTASSVILADIL
                     SDEVGYVSSTVTVGTASYTNGTVTFDLGNLTNLAVVTASISVTVTPAAAFSIINTCEV
                     SSSTADLVLSNNGAMATVTVVVDADGDGVPDGSDNCPDDYNPPTDWADLSNDWHVAEQ
                     EDYDLDGVGDVCDGCVWSNNPRAAVWYDMLGGTNYNSQVDFDLDGTQDACDNCPDDHN
                     PGQDDYDIDLMGDVCDPDDDNDGLPDDWEFLYFGDNLSAVTNVDSDTDGFTNWEEYLA
                     GTHPQDSNLYFRVESMSNLQSHAVYVESETGRIYRLLYADELANPVIWATGQVRAVTG
                     SLLSLTNSTTRTSRFYRVSVSFSP"
BASE COUNT         1941 a         3174 c         2676 g         2260 t
ORIGIN      
        1 ccagatgggt ttcgagcacc tgccccacgt tcatgcgcga gggcacaccc agcggattca
       61 gcacaatctc gaccggcgtt ccatcgggca ggaacggcat gtccgcctcc ggcacaatgc
      121 gggaaatcac acccttgttg ccgtgacgac cggccatctt gtcccccacc gacagctttt
      181 tcttgctcgc gatatacaca cgcacctgct tgatgatgcc ctggtctatc tcgtccccgc
      241 tgtccaggcg gtccatggcc cgttcctgct cttcctgcag gtccgcaaac ttgccggagt
      301 actcgccgat gatgccctga atcttgattt ggatggggct cgggtcaatc tcgatgttgt
      361 tgtgcgcggc cgccagcttg cgcagcagcg ttttcgtgat cttccggttc gccgggatga
      421 tgatctcgcc ggtctcgatg ttcacgacat caagcgggat cttctcgccc agcagaatgt
      481 tgctcagcgc ctcggtcaaa tcttccgtga gctgcttcga acgatcggca aattcctctt
      541 caatcgcctt cttctgcctg cgaatggccg tcggggtcat tttggtctgc tggacgggat
      601 tccgggccga aaccttgaca tccatcacga tcccgtaggt tccgctggga acccggagcg
      661 aggtgtcgcg cacatctgcg gccttttcac cgaaaatcgc ccgcagcagc cgctcttcgg
      721 gggccagctc ggtttcgctc ttcggcgtga ttttgccgac cagaatatca cccggcttca
      781 cttccgcgcc aatgcggatg acgccgtcat gctcgagatt ggccagggcg tcttctccca
      841 cattgggaat atcgcgcgtg atctcttcag gccccagctt ggtgtcgcgg gcgccgcact
      901 cgaaatcctc gatatgcaca gatgtgtaaa cgtcctcgcg aaccagcttc tcgctgatca
      961 gaatggcatc ctcgaaatta tatccgcacc agggcatgaa tgccacgaga acattgcggc
     1021 ccagagcaag ctcaccatgg ctggtgcccg ggccgttggc cagcacgtcg cccgccttca
     1081 cggtgtcgcc ctgcgccaca cagggtttct ggttgatgca ggttgcggca ttcgagcgca
     1141 tgaacttgcg cagttcatac acctgggctt cggctgccgg ggttttcttt gtcggcatct
     1201 cgccgttttt cgagacgatg atccggtcgg cggaaacgta agccaccttc ccggggcgtt
     1261 ccgccagcac catgacatga gaatcccggg ccacggggcc ctccatgccg gtcgccacat
     1321 aggggcgctc gggaaccagc agaggaaccg cctgacgctg catgttcgat cccatcagcg
     1381 cgcgattggc atcgtcgtgc tccaggaacg gaatcaggct tgcggccaca ctgaccagct
     1441 gcttgggcga aacatccatg tagtccacac gctcgcgcgg cacttcaagg aaatcgccgc
     1501 ggtaccgtac agaaacaagc tcgcgctcaa atgtgccttt ggccgtcagc ggcgcattca
     1561 cctgcgcgat gacatatttc tcctcctgat cggccgtcag ataataaact tcatcggaca
     1621 cccggccgtt gatgaccttc cggtatggac tctcgatgaa accgaattcg ttcacccgcg
     1681 catagcagct cagggaggaa atcagtccga tgttcggacc ttccggcgtc tcaatggggc
     1741 aaatgcggcc gtagtgactg gagtgcacgt cgcggacctc aaatcctgcg cgttcgcggc
     1801 tgagccctcc ggggcccagt gcgctcagac ggcgtttgtg cgtcagttcc gccagcggat
     1861 tggtctggtc catgaactgg ctcagctggc tgcggccgaa gaagtccttg atcacagccg
     1921 agagcgcttt cggattgatc aggcgctgcg gcgtcagttt gtccgtggcc gcgtcataca
     1981 gcgtcatgcg ctccttgacc agccgttccg tgcgggccag ccccacgcgg cactggcctt
     2041 ccagcagttc cccgatcgtg cgcacacggc ggctgcccag atgatcaata tcatcaatgg
     2101 tgccttcccc gcggcgcaga ttgatcagat agcgaacggc atccaccagg tcttccgtgc
     2161 ggagcaccag tgaatcattc ggctcctgtc ccagcttctg ctgaatcttg tagcggccta
     2221 cgcggcccag atcatagcgg cggggatcga agaaaatgcg ccggatcagc tgacgggcat
     2281 tggacaccgt aggaggatct cccggccgca gcttgtggta gatatccttc agcgcctcat
     2341 ccattgaatg cgcgggatcc ttctggatgc tcttcatgaa gattccttcg tcccacgaaa
     2401 cattcacgat atcagccgtt ttataaccgg ccgccttcat cgctttcacg gtttccggcg
     2461 tgagcgcgtc ataccggcgc gccagaatgg actgcgaatc ggcgtccaca atgtcgtctt
     2521 tcagcacttt gttcgccagc tcttcctcgg tgaagtcccg ggacagcttg agggtttcct
     2581 ccgtgtagaa aaggcggatc agctcttcat cgctgccgaa tcccagcgcc cgcaggaaag
     2641 tggtcgcgag gaacttccgg cgccgccgct tgcgctcaag atagatatac agcaggtcag
     2701 aggtgtcgaa ctgcacttca acccaggaac cgcggtcggg aataatccgg aaagagtaca
     2761 gaagcgagcc gttcgcatgg acactctgct caaagcagat gccgggtgaa cggtgcagct
     2821 ggctgacgat cacccgctcg gctccgttaa cgataaacga gccggacgag gagatcagcg
     2881 ggacctctcc catgtagacc acttcctcct gaacagactt cccctccttg aggcggaacg
     2941 tcacatgcat gggcgcggca aaggtttccg cctcgcgcag gcattccact tcggacattt
     3001 tcggctcgcc gatttcatat ttcacgaaat cgagcacgca ctgcccgtcg tagctctcga
     3061 tggggaacac ttccttgaag acggcctgaa gcccgacgcg ccggcggcgc gtgggagcca
     3121 catccatctg aaggaagtcc ttgtacgaag aagtctgaat ctcgatcaga tccggcagac
     3181 tgatcacatc tgccagcttc ccgaatttaa ttctgtcaac cattgaagtc acctctcggc
     3241 taatcgcaaa ggcgcccgaa ggcgccacac attctgtgtc cgctgaaaat cttgggcctg
     3301 aagaggccgt atttacttga tttcgacggt cgcgccagca gcctcgagct gtttcttcat
     3361 ggcatcggct tcagccttgt caacgccttc cttgacggtc ttcggggcgc catccaccag
     3421 atccttggcc tctttcaggc ccagaccggt gatcgcgcgg acttccttga tgacctgaat
     3481 cttctgggcg ccggcgctgg ccagcaccac cgcgaattcg gtcttctctt cagcggccgc
     3541 agcgccgccg cctgccgcag gagcagccgc catggccacg ggggccgcag cactcacacc
     3601 caggcggact tcaagggctt tgaccagctt ggaaagctcc agcacggaaa tcccttcaat
     3661 ccagtccaca aactcggccg ctttgccttc aaggacgggg gccgttttct cttcaacttt
     3721 atcggtcatg tttttactcc tcctacagca gctcttttac aagctcgttt gtttcttttc
     3781 ttcaacagct ttcaacacat acagcacact ggccaccttc tggctcaaca cacccgccag
     3841 gcgggtcatc ggcgcggcca gagtgcccag caggattgcc tgcataaccg gcttcgcggg
     3901 cagctccgcc agttcatcca cttcgtccgc cttcagcagc tggccttcca ccgcagccat
     3961 tttcaccgac ggcattttgt tcgcccgcat gaaatccttc agcacccggg cggtctccgc
     4021 cacatcaccg cggccggtga ccacggcgct ggggcccttg agggcggaat cgaagccgtc
     4081 cagggaaagc tccccggtga acttcttgaa catgcggttg tgaatcacgc gaaaacgcgc
     4141 gtccacgcga gccagctgcg tgcggagatt ttcggtctgc gccacgctga gcccgcgata
     4201 gtcggtcagg atcacaaaac ccgcgtcctt cgcttcggcg cgcatttcgt ctacaatgga
     4261 ttttttttct ggtctcatgg tactgattcc ttatgccgct ccggcgtaat ccttgacgtt
     4321 gatctgaagc ccgggtccca tcgtggaggt gaccacgcac ttcttcagga aaatgccctt
     4381 ggccacggcc gggcgggccg ccaccaccgc ttcaatcacc gcgcggcagt tttccgccag
     4441 ctgttcggct gtaaaggaca gtttcccgaa cgggaccatg acattgccat ggcggtccat
     4501 tttaaattcc acgcggccgg cctgaaactg cttcacggcc gtggccgtgt cgtctgacac
     4561 ggttccggtc ttcgggttgg gcatgagtcc gcggggcccc agcacctttc cgagcttgcg
     4621 aacctctttc atcgcttcag gcgtggccac ggcgatgtca aaatcagtcc agcctccggt
     4681 caccttggca atcagatcat ccatgcccac ttccgcggca cccgcctcgc gggccgcatc
     4741 cgcagccggt ccggttgcga aaaccaccac gcgaaccgtt ttgccgcttc catgaggaag
     4801 ggacacggtt ccgcgcacca tctggtccga cttcttcgga tccacaccca gccggaaagc
     4861 cacttccgcg gtctcgtcga acttggccgt ggaggacttt ttcagaacct caattgccgc
     4921 gtcgatcgaa taaacttcac tgcggttgag agccgtccgg acatccgtat atttttttcc
     4981 atgcgtagcc attgctattc cacctcaatc cccatgctgc gggccgtgcc ctccaccatc
     5041 cgcatggcct gttcaatatc ccttgcgttg agatcgtctt tcttcttctc cgcgatttcg
     5101 cggaccatcg ccttcgtgac cttgcccacc ttgtcccggt tgggcatggc cgaacccttg
     5161 gcaattccgg ccgccttttt cagcagcgtg gccgcgggcg ggctcttggt gataaacgtg
     5221 aaggacttgt cctgataaac cgtgatcacc accgggatga ccattcccgc ctgtttttgc
     5281 gtggccgcat tgaaggcctt gcagaactcc atgatgttca cgccctgctg gcccagggcc
     5341 gggcccacgg ggggcgcggg attggcctgg ccggccggga tctgcagttt aattattcct
     5401 gtgactttct ttgccatatc atttcacctg agctttattc gcgttcaacc tgccagtatt
     5461 ccagttccac aggagccgtc cggccgaaaa tggagactga tatcttcagc ttgccgtgat
     5521 ccggatccac ctcttccacc actccgctga agttgagaaa cgggccatcg gtcaccttca
     5581 ccggttctcc gggctcaaaa ttcaccttcg gcttgacctt ttccttcttc tcctcaatct
     5641 gattgagaat cacatccact tccgccggcc gcagcggcac cggattgtcc cccccgatga
     5701 acccgatgat tccctgggtt tcctgaatga aataccaggt tttttcattt attttgtggt
     5761 ccgcatcgta cagggccaga ttgatgagca catatcccgg atagaacttg cgggtcgtgg
     5821 tcatcttctt gccctgcttc acttccgaca ccttctcggt cgggatcagc acttcgccga
     5881 tatattcacc catctcttcg agaacggtgc ggcgttcgat gttttccttg accttgtttt
     5941 cctggccgga aagcgtatgt aaaacgaacc actgtttatc cattgtattc acccgggcgc
     6001 aatgccggtc ctatctgatc accagattca gaaatccctg agcgaccacg tcgcaaatgc
     6061 tcacataaat gcccaccagc agaaccgaca caatcacgat caccgtggac tccatgagtt
     6121 ccggacgggt cggccagttg cacttgcgga gttcctgcca tacttctccc ataaaattgc
     6181 ggaatccgct gacacctgct gacaatctgt tcatgctgac ttcctaactc tcaagtggca
     6241 ggccaggagg gaatcgaacc cccaaccccc ggttttggag accggtgctc tgccaattga
     6301 gctactggcc taccggaaaa catggcgtta tttggtctcc cggtgcagga tgtgcttcct
     6361 ctcgaaggga cagtatttct tcttttccat gcggcccgga gtcaggcgct tgtccttcgt
     6421 ggttgtataa ttgcgccgtt tgcactcggt gcaagcaagg gttatgattt ctctcgccat
     6481 gactgttcac attcctgcgg cctttgcttc cggccgcttt gcggcaagcc ccccctcgca
     6541 ggggggctcc cggtttttag ttctgattac tcaagaatct tcgacacacg gccggccccg
     6601 accgtccgtc cgccttcgcg gatggcgaac cgcatgtgct cttccatagc gatcggcgtc
     6661 agcagggtca cttccatgct cacgttgtcg cccggcatca ccatctccac gccctcgggc
     6721 aggatgatat ccccggtcac gtccgtcgtg cggaagtaga actgcgggcg gtatcccttg
     6781 aagaacgggg tatgacggcc gccctcatcc ttgctcagga catacacctc gccggtgaac
     6841 ttcgtgtggg gcgtgatgct tccgggcttc gccagcacct gaccgcgctc cacatcgtcc
     6901 ttcttcacgc cgcgaagcag gcagcccacg ttgtcgccgg cctggccctg gtccagcagc
     6961 ttgcggaaca tttccacgcc cgtgacggtc gtcttggtgg tgtcgcgaat gcccacgatg
     7021 gcgacttcct caccgacctt gatgatcccg cgctcaatac ggccggtcac cacggttccg
     7081 cggccttcga tcgagaacac gtcttcgatc ggcatcagga agggctggtc aagaggacgg
     7141 ggaggctcct tcacccaggc gtccagagca tccatcagct tctggatgca ctccgcatcc
     7201 gggccgctcg gattctcggt cgccttcagc gccgagccgc gaatgatcgg ggtgtcgtcg
     7261 ccggggaatt cgtacttgtt cagcagttcg cggatttcga gctccacgag atccagaagt
     7321 tccggatcat ccaccgtgtc caccttgttc aggaaaacga cgatggaagg cacgcccacc
     7381 tggcgggcga gaagaatgtg ctcacgcgtc tggggcatcg ggccgtcgga agcgctcacc
     7441 accagaatgg cgccgtccat ctgcgccgcg ccggtgatca tgttcttcac atagtccgca
     7501 tggccggggc agtccacatg ggcgtaatgc cggttggccg actgatattc cacgtgcgag
     7561 gtcgcaatcg tcagaatctt cgtcgagtcg cggcggccct gtgactcgga ggctttcgcc
     7621 acctggtcat aagaaatgaa cgtcgcgagg cccttggctt cctgcacctt ggtgatggcg
     7681 gccgtcagcg tggttttgcc gtggtcaaca tgaccaatcg tgcccacgtt gacgtggggt
     7741 tttgttcttt caaatttttc ctttgccatg gaactctcct cctagcgtct ttctctgttg
     7801 ttaatatgtt cagtttctct tctgctaaaa tccatcggcc ctgctggagc ccacgagcgg
     7861 gattgaaccg ctgacctcat ccttaccaag gatgtgctct accaactgag ctacgtgggc
     7921 cgcgctttcg ccgtctgccg gcaggacacg catccgccgc cgctacaccc tgcccatctc
     7981 actcagcaga cataatcaca caggacatcc gttcccctca tttcttattc ccagcagaaa
     8041 aatgagagac cgtgccctgc tcaacctaat ctagtctgtc ccgccgctgc ggtttcccgc
     8101 atcaacagtg cggcgtactt tacaggaaac agtacaggag tcaaatggtt tctgaaaaat
     8161 attttgcaaa ataattccgg cgggaaaatc aggcggaagg ccccagatac atctgcagga
     8221 ggctgttcac atcatccaca ctgaacggtt tgtccagata ggcgcggcat cccagctcct
     8281 ggatatgctc aatcgtttta tcgtccagat aggcggaaac cacgatgacc ggcatatccg
     8341 ggagcatctc ccgggccagc gtaatcagct gaatcccgtc caccggtttc atccggagat
     8401 cggtcagcat caggtcgaac tgttcgccgg cctgaatgcg gtccgccgcc tcctggctgt
     8461 ccgtgagcgc cacgacctcg ccgccgcgca cggagaccag acactgcagc gtttcaataa
     8521 tcgccgcatc atcatccact aaaagtactt taggcataac cataggaata tactattccg
     8581 ctccgctgtt tgcatccttt ttgacgcgca tttcagaaat ttttctacgg ggaaaacgac
     8641 accgatacgc ggtaaaaccg gctcgttcgg gtcgtgctgt tggtcaggct cagcaaagag
     8701 ccggtcacgg cccgcacctg cccggtggcc cagatcacgg gattcgccag ttcatccgca
     8761 tacaaaagcc ggtaaatccg gccggtctcg gattccacat agacggcatg gctctgcaga
     8821 ttcgacatgg attccacgcg gaaataaaga ttggagtcct gcggatgggt gccggcaaga
     8881 tactcctccc agttggtgaa accgtccgta tccgaatcca cattcgtcac ggcgctcaag
     8941 ttgtcgccga aataaaggaa ctcccagtcg tccggcaggc cgtcattgtc gtcgtccgga
     9001 tcgcagacat cgcccatcaa atcaatgtca taatcatcct gtcccggatt gtgatcatcc
     9061 ggacagttgt cacaggcgtc ctgagtgccg tccaggtcga aatccacctg tgaattgtaa
     9121 ttggtgccgc ccagcatatc gtaccacacc gccgcacgcg gattgttgct ccacacacat
     9181 ccgtcgcaca cgtctcccac gccatccaga tcataatcct cctgctcggc gacatgccag
     9241 tcgttagaca aatcggccca gtctgtcgga ggattgtaat catccgggca gttatccgac
     9301 ccgtccggca ccccgtcacc gtcggcgtcc accaccacag tcacggtcgc catggcgccg
     9361 ttgttgctca gtacgagatc ggccgtggag ctgctgacct cgcaggtgtt gatgatgctg
     9421 aaggccgccg cgggcgtgac cgtgaccgaa atgctggccg ttaccaccgc aagattggtg
     9481 agatttccga gatcaaatgt aaccgtcccg tttgtgtagc ttgcggtgcc caccgtgacg
     9541 gtggaggaaa catagccgac ctcgtccgaa aggatatccg ccaggatcac cgacgaggcc
     9601 gtgtccgggc ccctgttcgt gacatagaga tggtattcaa actgctggcc gtccatcacc
     9661 acattggtcg aggccgtctt cttcacagcc agatcggcaa acagcatccg gtagactgtc
     9721 agcttgttct gcgagtcgcc cacggcaaga tatttcccgt ccgatgacca gcgaaccgaa
     9781 ttcacggcgg aaatatgctc attggaattc agcagccgga gcgcgccgga caaccggttg
     9841 tattcataca agtacagttc cggaccggtg gaattcagct ccgttcccac tgccagaaaa
     9901 tcaccggccg gagaccagtc caccgtcagc ggactggcat ttagaagagt gctgacctgc
     9961 aattcaagct gtttggctgt cgagttgtag gaatatacag agaaggcctt gtaggcggga
    10021 atcagggata aggccccggc agccagaagg g
//
LOCUS       DAOQWR010000097         9952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7276_length_9952_cov_5.079317, whole genome shotgun sequence.
ACCESSION   DAOQWR010000097 DAOQWR010000000
VERSION     DAOQWR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7276_length_9952_cov_5.079317"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..592
                     /locus_tag="PKK36_08295"
     CDS             398..592
                     /locus_tag="PKK36_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94592.1"
                     /translation="MKWFFMAVMIAALAGCSREARKAADEAVDDVILQKTTIEAGQAA
                     KQKVQEINAIRRESFKEMEE"
     gene            complement(573..1919)
                     /gene="trmFO"
                     /locus_tag="PKK36_08300"
     CDS             complement(573..1919)
                     /gene="trmFO"
                     /locus_tag="PKK36_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNR94593.1"
                     /translation="MSRLLTVAGGGLAGSEAAWQAARQGIQVRLYEMRPDQQTPAHVS
                     GSLAELVCSNSLGSALPDRATGLLQNELRAMGSLLMRCADACAVPAGGARAVDRAAFA
                     GMVTGEISGHPNIRIIREEVTAIPEGPAVIATGPLTSSRFSDALAALTGREHFSFFDA
                     LAPLITADSINMDIAFRASRYGRGEQGEGDYINCPLNREEYDRFVDALIAAERIPLKT
                     FEEDVEKGVTAGMNKFFEGCLPVEILARRGRDALAFGPMRPIGLDDPRTGRRPRAILQ
                     LRQDNVAGSLYNMVGFQTNLRRSEQERVFRMIPGLEGAEFARHGQMHRNTFLAAPALL
                     NSTLQMKEHGNLFIAGQLTGVEGYAGSIGTGLLAGLNAARLIKGGKLLELPQVTMLGA
                     LVHYITHADLKTFQPMKANFGIMPALETEKRLGKQQRGAAYAERALAALNITLPSP"
     gene            complement(1916..2992)
                     /locus_tag="PKK36_08305"
     CDS             complement(1916..2992)
                     /locus_tag="PKK36_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94594.1"
                     /translation="MNKPFDLQKLLKDVFRPGMDERVVVMTDVPAETDNPEWQERRLM
                     ADEWRTAFIQMGLETLPLLTYPSTGAANADLPEQGWLDDAPVEIADVLKSCTIVVAMT
                     EYSATAPLSAFVRESGGKLRAASMPGVLRRMEETALAADYQQIAIRVHQLTERLTRAE
                     GAQVVFSTGHEMFFDLRWRSGHGDDGMCGPGKAFPLINLPSGEAYIVPYEGERPGEPS
                     RTRGQIPVALNGETVLLEIRANRIEKISGGEEARKLNEFFTRDPARRNVAELGLGCND
                     RAVVSGSVLEDEKAGFHWAYGRSEHLGGIISPKDFSAPHHVVHHDVVYAVGSPITIAR
                     LTLQYHAGAEEVIMQDGRYTIFAE"
     gene            3106..4260
                     /locus_tag="PKK36_08310"
     CDS             3106..4260
                     /locus_tag="PKK36_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94595.1"
                     /translation="MKVLFLVPEPFFVIRGSSVTVRAWIRTLTEAGHKVDMICHPEGR
                     DEDLGAAAVRRTWSCAGSLLLRDLCMFFVAAAMCIRARYDVIHALNQSAVSAVLLKRL
                     FKLRVVYDLDLDPAEVFGDGARLTRGLRSAAAGRLERSVLKRADAVVVSSATRAERAR
                     AAGAVAKVEEIDDPPYDCPMGENRDGGVQLRQELGIGTAPVVLYTGSFADSQQMDLLL
                     RAARLVHDQQPEVRFLMAGEKTAEIPRLARLAATLDIADRCILTGLKSMGQISALLAA
                     ASVLVSPRLRPSRTFSKLFTYMLSNRVIVATRMADQARVLDSGCAILTLPQPDELAGG
                     ILRALDEPLLSRGLAREAWSRAAAEHTMASFKHRVRGFYQQLAAGEKTSA"
     gene            complement(4271..4849)
                     /gene="yfbR"
                     /locus_tag="PKK36_08315"
     CDS             complement(4271..4849)
                     /gene="yfbR"
                     /locus_tag="PKK36_08315"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HNR94596.1"
                     /translation="MSHFFAYVSRMKLIQRWGLMRNTQTENIQEHSLQVAMVAHTLAV
                     IRNRLYGGNVNPERVATLAMYHDCSEVITGDLPTPIKNFNREIVTAYKKIEHLACERI
                     YKMLPEELREDYRDIFFPCEQDDEHWQLVKAADKLCAYLKCLEELQAGNNGFLKAERA
                     VMAGLMKMNMPEVTYFVETFSPSFSLTLDELN"
     gene            complement(4867..6783)
                     /locus_tag="PKK36_08320"
     CDS             complement(4867..6783)
                     /locus_tag="PKK36_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94597.1"
                     /translation="MRRFVFIVLIASAMLTAALLSCGRHPGEREYNQGLRELQAGRWV
                     RAKNLFEKAISRRPGSRESAAACNGLGIANWHLKQIEAAAQAFEESLRLDPEFMEPSF
                     NLGVLLYRSGDAGQALQRLEEAALNDKDGTRALEYAAYICMQMEDWSEARRMLQRAGS
                     RSASSPRIATMLSLADMKLAGTEMAGFHLMQALDKNAEYGPALYNLGLLHKDYLKNPA
                     QAFSFFSQYVDLNPEGPRAEAAKAHLEQCRSAAGSENDTVVAEKPDSPAAPPMRPATL
                     VVETIESSLQAARKAAADDKPRDALNHFLKAAAEAGRSGATARQRLILEEAADKCPNL
                     EAAHASLGTYWLTRGKSSEAMSAFLKAVEIDARQAPSQMGLANAALAEGEYDIAASAL
                     KTAVALAPDNPDALWSLAELYESDELGWADRAMECYRQFEQLFPGDPRVLKARAKLFS
                     PAAAPEGQPPAPQAGLSPASDAMQRLQLKRPMQRLRSEAMQAFNRGYSFQLQEDWDRA
                     IYYYRHAIENDDTFLNAYFSLGVVYQLNGEPALAKDAYLAGLDQKPDWTDARYNLALL
                     CMELRQYAEAEQQARKTLELDPGHAQSHYILGMIYERNPDRKALACQHWQRFIELAPN
                     DPLVADARKRLKDR"
     gene            complement(6785..7180)
                     /locus_tag="PKK36_08325"
     CDS             complement(6785..7180)
                     /locus_tag="PKK36_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNR94598.1"
                     /translation="MKEVEIQTQERSQFLDITAAVREAVQESGIRDGRATVFVPHTTA
                     GITINENADPDVVRDMLSVLDELVPWRGNYRHAEGNTAAHVKSSLMGSSAAVLVSAGR
                     LQLGVWQSIWFCEFDGPRTRKVLIEIHKG"
     gene            7292..8521
                     /locus_tag="PKK36_08330"
     CDS             7292..8521
                     /locus_tag="PKK36_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNR94599.1"
                     /translation="MLLLVLQLLLLIALLICSAFFSSSEVAFFSLNPLQISRLEARDP
                     SAAARIREILVRPTKLLSTILTGNTLVNIVASAVGYSIVGRFGGAWAAVITIPLMTVL
                     LLIFGEIGPKRLAIQRPVRLAVLYTPILNVVIRISRPLRHMLEAITRSLAWLFHPRGH
                     LLSGEEVASVMEVSHQKGLLDADERAMLSGIMRLEKLRASDVMTPRMDIVGIDLDDLP
                     DDPVAVARSAKVPYLPVYREIPDHIEGILDVGAFLMDPDHQIERACVAPFYVPEAAPL
                     NKLLGQFRREKRRLAVVVDEYGGTAGLISRGDIIEEITGHIDGAEPYVIETLAPGKWL
                     VDGQISLAELNQKLGLRLSEEGVDRFSGWIISKAGRLPRFGEAVLADGLRATVRKMRR
                     HRIELVMLEKLAGEERA"
     gene            8518..9486
                     /locus_tag="PKK36_08335"
     CDS             8518..9486
                     /locus_tag="PKK36_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNR94600.1"
                     /translation="MIALEIILILAFLAAGGFFAGMETGVVATNRLRLQHLLHHKAPG
                     APVLKYFLDHPDHLLGTTLVGTNLSHVSISVLSTHVAARLFGQTGTVLAPVVMTLVIL
                     VFAEYLPKAFFQSNPSARTLPVISLLDVAGRIFHPVNRAIMSVTSLLFPSLPAGEGES
                     AAFITREELKRLTAEAASDGKLGRARRMMIERVFDLPLKTCRQIMIPTEHMVAVELSA
                     PREEIIARAHDSGYSRMPVFEGSRAGIRGIVHILDVLGDSSGSPRTARDYMRPPSYVA
                     ADTPADDLLARMRVTRQPMLIVRDEATQVLGLVTTEDVLEEIFGEL"
     gene            complement(9497..>9952)
                     /locus_tag="PKK36_08340"
     CDS             complement(9497..>9952)
                     /locus_tag="PKK36_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HNR94601.1"
                     /translation="LPESDWMRSQAPALAALYDRFAVIGSAPPPDVSPHAPGTAGWPL
                     TVIHLLGTALVIAVAEEFFWRGFIYRWLLDRDFIGVPAGRLDWQMLLLSSLVFGFEHD
                     RWLAGFAAGIVYALVFVRTRDIWSACAAHITTNLLLGLYVLATGAYAFW"
BASE COUNT         1837 a         2997 c         3074 g         2044 t
ORIGIN      
        1 cctcaaactt ttcaatctgc acctctccag acaggaactt gtcggatgaa ccccaaaaac
       61 gcaaaaatcg ggcaaaatta tgaaaaatac ggccgcataa tgtttttcgc atcaaaatag
      121 tactaattta gtactatata aaaccgggcc ggaaaaaatt tccaaatact gcccaaaagc
      181 gtgaaaaacg ggttcttcgt cacttgtcgt ggaggtttct gcgctcaaat cagcatcctg
      241 gcgtgaaatg atgaacattg cgccgccttc gcggcacttc ggagtgcgcc agtctcgcct
      301 gcgactcgga cacgccgctt tcacacgaat tcacatgcgg atgaggtttc acaagcgggg
      361 ggattccggt acaatgcgga ttgaaaggat gtggattatg aaatggtttt tcatggccgt
      421 gatgattgcg gcgctggccg gatgcagccg ggaggcacgg aaagcggcgg atgaggcggt
      481 ggatgatgtg attctccaga agaccaccat cgaggccggg caggccgcaa agcagaaggt
      541 tcaggagatc aacgccatcc ggcgcgagag tttcaaggag atggaagagt gatgttcagc
      601 gcggcgagcg cccgttcggc ataggccgcg ccgcgctgct gcttgccgag ccgcttttcc
      661 gtttccagcg cgggcatgat cccgaagttg gccttcatcg gctgaaaggt tttcaagtcc
      721 gcgtgcgtga tgtagtgcac cagcgccccc agcatggtga cctgcggaag ttccagaagt
      781 ttcccgcctt ttatgagccg cgccgcattc agtccggcca gaaggcccgt gccgatgctg
      841 cccgcatagc cctcgacgcc ggtgagctgg ccggcgatga agagattgcc gtgctctttc
      901 atctgcagcg ttgaattcag cagggccggc gctgccagaa aggtattccg gtgcatttgc
      961 ccgtggcgcg cgaattcggc gccctccagt cccgggatca tgcggaagac gcgttcctgc
     1021 tcgctccgcc gcagattggt ctggaatccc accatgttgt agagcgagcc ggccacattg
     1081 tcctgccgca gctgcagaat tgcgcgcggc cggcggccgg tgcgcggatc atcaagcccg
     1141 atcggccgca tcggcccgaa ggccagcgca tcccgcccgc gccgggccag gatttcgaca
     1201 ggcaggcatc cctcgaaaaa tttgttcatg cccgcggtga cgcccttctc gacatcctcc
     1261 tcgaaggttt tcagcggaat gcgctccgcg gcgatgagcg catccacaaa ccggtcgtat
     1321 tcctcccggt tcagcggaca gttgatgtaa tcgccctcgc cctgttcgcc gcgtccgtag
     1381 cgcgaggcgc gaaaggcgat gtccatgttg atggagtctg ccgtgatcag cggggccagc
     1441 gcatcaaaaa aagaaaaatg ctcgcgtccc gtcagcgcgg ccagcgcatc cgaaaaccgg
     1501 ctggatgtca gcgggccggt ggcgatgacg gcgggccctt cggggatggc cgtgacttct
     1561 tcgcggatga tgcggatgtt cggatgtccg gatatttccc ctgtcaccat cccggcgaat
     1621 gccgcgcggt ccacggcgcg cgcgccgccg gccggcaccg cgcaggcatc ggcgcagcgc
     1681 atcagcagcg accccatggc tctgagttcg ttctgcagca gtccggtggc gcggtcgggc
     1741 agggcggatc cgagactgtt ggagcagacc agttccgcga ggctgccgct gacatgcgcc
     1801 ggggtctgct ggtccggccg catttcataa agccggacct ggattccctg ccgggcggcc
     1861 tgccatgcgg cctcgcttcc ggccagcccg ccgcccgcga ctgtgagaag gcggctcatt
     1921 cagcgaagat ggtgtaacgg ccatcctgca tgatgacttc ttctgcgccg gcgtgatact
     1981 gcagcgtcag ccgggcgatc gtaatggggc ttccgaccgc gtagaccaca tcatgatgaa
     2041 caacgtgatg aggcgcggaa aaatctttcg ggctgattat tccacccagg tgttcgctgc
     2101 ggccataggc ccagtggaat ccggcctttt cgtcctcaag cacactcccg gaaacaacgg
     2161 cgcggtcgtt gcagccgagt cccagttcgg ccacattgcg ccgggccggg tcgcgggtga
     2221 aaaactcatt cagcttccgg gcctcttcgc cgccggagat tttttcaatc cgattggccc
     2281 ggatttccag aaggacggtt tccccgttca gtgccacggg aatctgcccg cgggtccggc
     2341 tgggttctcc ggggcgttcg ccttcgtagg gcacgatata ggcttctccg ctcggcagat
     2401 tgatcagcgg gaaggccttg ccgggcccgc acatgccgtc atccccatga cccgagcgcc
     2461 agcgcagatc gaaaaacatt tcatgcccgg ttgaaaagac aacctgagcg ccttcggcgc
     2521 gggtcaagcg ttcggtgagc tgatgaaccc tgatggcaat ctgctggtaa tccgccgcaa
     2581 gggcggtttc ttccatccgc cgcagaacgc ccggcatgct ggccgcgcga agtttccccc
     2641 cgctttctct gacaaaggcc gaaagcggcg ccgtggcgga atattccgtc atggccacga
     2701 cgatggtgca cgacttcagc acatcggcaa tctcgaccgg ggcatcatcc agccagccct
     2761 gctccggaag atcggcattg gccgcgcccg tggacggata agtcagcagg ggaagcgttt
     2821 ccagccccat ttggatgaat gcggtgcgcc actcatcggc catcaggcgg cgctcctgcc
     2881 actccggatt gtcggtttcg gccggaacat cggtcatgac gacgacccgc tcgtccattc
     2941 ccggccggaa gacgtctttc agaagcttct gcagatcaaa tggtttattc atcacatatc
     3001 ctcttcataa agactaacat ctggcgccgg gcgcgtcaaa ccggccgggg ccggtcgaaa
     3061 tttagcttga ggcggctcgc tcaaagaaac tatacgtgaa aacagatgaa agtgctgttt
     3121 ctggttcccg aacctttttt tgtgatccgc ggttcgtctg tgacggtccg ggcatggatc
     3181 aggacgctga ctgaagcggg acacaaggtg gatatgatct gccatcccga agggcgcgac
     3241 gaagacctcg gtgccgccgc ggtccggcgc acctggtcct gtgcgggcag tcttctgctg
     3301 cgcgacctgt gcatgttctt cgtggccgcg gccatgtgca tccgcgcccg ctacgatgtg
     3361 attcatgccc tcaaccagag cgccgtttcg gccgtcctgc tgaagagact gttcaagctc
     3421 agggtggttt atgatctgga ccttgatccg gcggaggtgt ttggagacgg ggcgcggctg
     3481 acccgcggcc tcagatccgc cgcggccggc cgcctggagc ggtccgtgct gaaacgtgcc
     3541 gatgccgtgg tggtttcctc cgcgacccgc gcggagcgcg cccgggcggc gggagctgtc
     3601 gcgaaagttg aggagattga tgacccgccc tatgactgcc cgatgggcga aaaccgggac
     3661 ggaggcgttc aactgcgcca ggagctgggc attggcacgg cgccggtggt gctttatacc
     3721 ggctcttttg ccgactccca gcagatggat cttcttctgc gcgcggcgcg actggtgcac
     3781 gaccagcagc cggaagtccg tttcctgatg gcgggcgaga aaacggcgga gattccccgg
     3841 ctggccagac tggcggccac actggatatc gccgaccgct gcatcttgac cgggctgaag
     3901 tcaatgggtc agatctccgc gctgctggcc gcggccagcg tgctggtttc accccgtctg
     3961 cgcccgtcgc gaaccttctc caagctcttc acgtatatgc tcagcaaccg ggtcattgtg
     4021 gccacgcgca tggcggatca ggcccgtgtg ctggattcag gctgcgcgat cctgacgctg
     4081 ccgcagccgg atgaactggc cggcgggatt ctccgcgccc tggatgaacc gctcttatcc
     4141 cgcggccttg cgcgcgaggc ctggtctcgc gcggctgcgg aacacaccat ggccagcttc
     4201 aagcacaggg ttcgcggttt ctatcagcag ctggctgcgg gagaaaaaac ctccgcctga
     4261 tgcgggaaag tcagttcagc tcgtcgaggg tcagggagaa gctgggggag aacgtttcca
     4321 caaaataggt cacttcgggc atgttcatct tcatgagacc ggccatgacg gcccgctcgg
     4381 ccttcaggaa gccgttgttg cccgcctgca gttcctcaag gcacttcaga taggcgcaga
     4441 gcttgtcggc ggccttcacc agctgccagt gctcatcgtc ctgctcgcag gggaagaaga
     4501 tgtcccggta gtcctcccgc agctcctccg ggagcatctt gtagatgcgt tcacaggcca
     4561 ggtgttcaat cttcttgtag gcggtgacga tttcgcggtt gaagttcttg atgggggtcg
     4621 gcagatcccc ggtgattacc tcgctgcagt catgatacat cgcgagggtg gccacccgct
     4681 cgggattcac attcccgccg tacaacctgt ttcggatgac cgcgagggta tgggccacca
     4741 tggccacctg caggctgtgc tcctgaatgt tttcagtctg ggtgttgcgc atcagccccc
     4801 agcgctggat cagtttcatg cgggaaacat atgcaaaaaa atggctcatg gacggtctct
     4861 cctctgtcac cggtctttca ggcgcttgcg cgcgtccgcc accagcggat cgttcggggc
     4921 cagttcgatg aaccgctgcc agtgctgaca ggcaagggct ttgcggtccg gattgcgttc
     4981 ataaatcatg ccgaggatgt agtgcgactg tgcatgtccg ggatccagtt caagagtctt
     5041 gcgggcctgc tgttcggctt ccgcgtactg ccggagctcc atgcacagca gggccaggtt
     5101 gtaacgggca tccgtccagt ccggcttctg gtccagcccg gccagatagg catccttcgc
     5161 gagcgccggc tctccgttga gctgataaac cacgccgaga ctgaaatagg cgtttaaaaa
     5221 agtgtcatcg ttttcaatgg catgacggta atagtagatc gcccggtccc agtcctcctg
     5281 aagctggaaa ctgtagcccc ggttgaaggc ctgcatcgcc tcgctgcgga gccgctgcat
     5341 gggccgcttc agctgaagcc tctgcatggc atcactggcc ggtgacaagc cggcctgcgg
     5401 cgcaggcggc tggccttccg gcgcggcggc cgggctgaac agctttgccc gcgccttgag
     5461 cacgcggggg tccccgggga agagctgttc aaactgacgg tagcattcca tggcgcggtc
     5521 agcccagccc agttcatcgc tctcgtaaag ttcggccagc gaccagagcg cgtccggatt
     5581 gtccggcgca agcgccactg cggttttcag cgcgctggcc gcgatgtcat attcgccctc
     5641 ggccagggcc gcgttggcga ggcccatctg tgaaggggcc tgccgtgcat caatttcgac
     5701 cgccttcaaa aatgcgctca tggcttcgct gcttttcccc cgggtgagcc agtaagtccc
     5761 gagcgaggca tgggccgcct ccaggttcgg gcacttgtcc gccgcttcct cgagaatgag
     5821 tctctggcgc gcggtggcgc cgctgcggcc ggcttcggcc gccgccttga gaaaatggtt
     5881 cagcgcgtca cgcggcttgt cgtcggcagc ggccttccgg gccgcctgca gactggactc
     5941 gatggtttcc accacaagag ttgccggccg catgggcggc gcagccgggc tgtccggctt
     6001 ttcagcaacc acagtatcgt tttcgcttcc ggctgcgctg cggcactgct caagatgagc
     6061 ttttgcggct tctgcccggg gtccttccgg attcaggtcc acatattgag aaaaaaacga
     6121 aaaggcctgt gccggatttt tcaggtaatc cttgtgaagg aggcccaggt tgtacagtgc
     6181 cgggccatat tccgcattct tgtccagcgc ctgcatcagg tggaatccgg ccatttccgt
     6241 ccccgccagc ttcatatccg cgagggaaag catggtggcg atccgggggg aactggccga
     6301 gcgcgaaccg gcgcgctgaa gcatgcggcg ggcctcggac cagtcttcca tctgcatgca
     6361 gatgtatgcc gcatattcaa gcgcgcgggt gccgtctttg tcattcagtg cggcctcttc
     6421 gagccgctgc agggcctggc ccgcatctcc gctccggtac agcagcaccc ccagattgaa
     6481 ggatggctcc atgaactccg ggtccaggcg caggctttct tcaaaggcct gcgcggcggc
     6541 ttcgatctgc ttcaggtgcc agttggcgat gccaagcccg ttgcaggccg ctgcgctttc
     6601 ccggctcccc ggccgccggg agatggcctt ttcaaaaaga ttttttgcgc gcacccaccg
     6661 cccggcctgc agttcgcgca ggccctggtt gtattcacgc tcgcccggat ggcgtccgca
     6721 gctcaaaagc gccgcggtca gcatcgctga ggcaatcagt acaataaaga caaatctccg
     6781 catgttatcc cttatgaatt tctatcagca ccttgcgcgt gcgcgggccg tcgaactcgc
     6841 agaaccagat actctgccac acgcccagct gcagccggcc tgcggaaacc agaaccgccg
     6901 ccgacgatcc catcaggctg ctcttcacat gcgccgcggt atttccttcc gcgtgccgat
     6961 agttcccgcg ccaagggaca agttcgtcca gcacggacag catgtcgcgc acgacatccg
     7021 gatcggcatt ttcgttgatg gttattccag ccgtggtgtg cgggacaaaa acagttgccc
     7081 ggccgtcccg gatgcccgac tcctgcacgg cctcgcgcac cgccgcggtg atatcgagaa
     7141 actgggacct ctcctgcgtc tgtatttcca cttctttcat gagatgccca ttctatttca
     7201 taaaataatt aatgcaagac ggatgaggat gcgttattaa aatggatttg cctgcagagt
     7261 cactgcaagg cttcgggccg gaatttatga tatgctgctg ctggttctac aactgctgct
     7321 gctgattgca ctgctgatct gctccgcgtt cttttcgagc tcggaagtgg cctttttttc
     7381 tctgaacccc ctgcagatca gccggcttga ggcgagagat ccgtccgcgg ccgcccggat
     7441 tcgtgaaata cttgtccgcc cgaccaagct tctttcaacc atcctgacgg gcaacacgct
     7501 ggtgaatatt gtggcttcgg cggttggcta ttcgattgtg ggccggtttg gcggagcctg
     7561 ggccgcagtc atcacgattc ccctcatgac ggtattgctg ctgattttcg gagaaatcgg
     7621 gcccaagcgg ctggcgatcc agcgtccggt gcgcctggct gtgctctata ccccgattct
     7681 caacgtggtg atccgcatca gccggccgct gcggcacatg ctcgaagcca tcacgcggag
     7741 cctggcatgg ctctttcacc cgcggggtca tctgctgagc ggcgaagagg tggccagcgt
     7801 gatggaggta agccatcaga agggcctgct ggacgccgat gagcgggcca tgctcagcgg
     7861 catcatgcgc ctggaaaagc tgcgcgccag tgatgtgatg acccctcgca tggatattgt
     7921 cggcattgac ctggatgatc ttcccgatga ccctgttgct gttgcccggt ctgcgaaagt
     7981 tccatatctg ccggtgtatc gggagattcc ggatcatatc gaagggattc tggatgtggg
     8041 cgccttcctg atggatcccg atcatcagat cgagcgcgca tgcgtcgccc ccttttatgt
     8101 gccggaagcc gctccactga acaagcttct ggggcagttc cgccgggaga agagacggct
     8161 ggcggtcgtg gtggatgaat acggcgggac ggctggtctc atcagtcgcg gtgacatcat
     8221 tgaggagatt accggtcata ttgacggggc ggagccgtat gtcattgaaa cactggctcc
     8281 aggcaaatgg ctggtcgatg gacagatcag tctggcggaa ctcaatcaga aactgggcct
     8341 tcggctgagc gaagagggag ttgaccggtt ctcgggctgg attatcagca aggccggccg
     8401 tctgccccgt tttggtgaag cggttttggc ggatgggctg cgggccacag tccgcaaaat
     8461 gcgccgccac cgcattgagc tggtgatgct ggagaaactg gcgggagagg agagggcatg
     8521 attgctctgg agataatcct gattctcgct tttctggccg cgggcggatt ttttgccgga
     8581 atggaaaccg gggtggtggc cacgaatcgt ctgcgtctgc agcatctgct gcatcacaag
     8641 gcgcccggcg ctccggtcct caagtatttc ctggaccacc cggatcatct tctgggcacg
     8701 acgctggtgg ggaccaattt gagccacgtg tccatttcgg tgctgtctac gcatgtggcc
     8761 gcccggctct tcgggcaaac cggcacagtg cttgcgcctg tggtcatgac gctcgtgatt
     8821 ctcgtgtttg ctgaatatct cccgaaagct tttttccaga gcaatccgtc ggcgcgcacg
     8881 cttccggtga tcagcctcct ggatgtggcc ggccgaatat ttcatcccgt gaaccgcgcg
     8941 atcatgtctg tcacaagtct gcttttcccg tcgctgccgg cgggtgaggg ggagtctgcg
     9001 gccttcatca cgcgcgagga actgaagcga ctcacggccg aggccgccag cgacgggaaa
     9061 ctgggccgcg cccgccgtat gatgattgag cgcgtgtttg atctgccttt aaagacctgt
     9121 cgccagatca tgattccgac agagcacatg gtggctgttg aactttccgc cccgcgcgag
     9181 gaaatcatcg cgcgtgcaca cgacagcggt tattcacgca tgcccgtgtt tgagggaagc
     9241 cgggccggaa tcaggggcat tgtgcacatc ctggacgtgc tgggtgacag cagcggaagt
     9301 cctcggaccg cccgcgatta tatgcgcccc cccagctatg tcgccgcgga taccccggcc
     9361 gatgacctgc tggcccgcat gcgcgtgacc cggcagccca tgctgattgt gagagatgag
     9421 gcgactcaag ttctcgggct ggtgaccacc gaggatgtgc tggaggagat attcggcgag
     9481 ctctaagagg atatcatcac cagaaagcgt atgcgccggt ggcgagcaca tagaggccga
     9541 ggagcagatt ggtggtgatg tgggctgcgc aagcgctcca gatgtcgcgg gttcggacga
     9601 aaaccagtgc ataaacaatt cccgcggcaa acccggccag ccagcggtcg tgctcgaaac
     9661 caaacacaag cgaagaaagc agaagcatct gccagtccag ccgtcccgcg ggcaccccga
     9721 taaagtcgcg atccagaagc cagcgataga tgaatccccg ccagaaaaat tcttcggcaa
     9781 cggcaattac gagagcggtt ccaaggaggt gaatcacggt cagcggccag cccgcggttc
     9841 ccggcgcatg cggcgacacg tccggcgggg gcgcgctgcc gatgactgca aagcggtcat
     9901 acagcgcggc cagcgccgga gcctgcgaac gcatccagtc tgattccgga ag
//
LOCUS       DAOQWR010000098         9747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7462_length_9747_cov_5.144552, whole genome shotgun sequence.
ACCESSION   DAOQWR010000098 DAOQWR010000000
VERSION     DAOQWR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7462_length_9747_cov_5.144552"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="PKK36_08345"
     CDS             <1..2184
                     /locus_tag="PKK36_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012823505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR94602.1"
                     /translation="ALKADYVLVYRNRKLAVVEAKAWDEELTEGVGQAKDYADKLKIR
                     FAYSTNGQGIYGIDMKPGKEGAVSAYPSPEELWQMTFAEQNAWRDRFSTVPFEDKGGS
                     HPSRYYQDTAIENVMEAIAAGKNRILLTLATGTGKTFIAFQIAWKLFHSRWNLSGEPT
                     RRPRILFLADRNILANQAYNAFSAFPEDALVRIAPDSIRKKGRVPTNGSLFFTIFQTF
                     MSGQSEDGEPSPYFGEYPKDFFDFIVIDECHRGGANDESNWRDIMEYFSPAVQLGLTA
                     TPKRTENVDTYRYFGEPVYIYSLKDGINDGFLTPFKVRQIQTTLDEYVYTPDDSVVEG
                     EIESRKTYTESDFNKIIEIKEREKKRVQIFMEQINQNEKTIVFCATQDHALAVRDLIN
                     QMKTSKDPNYCQRVTANDGELGEQHLRAFQDNEKTIPTILTTSQKLSTGVDARNIRNI
                     VLMRPINQIIEFKQIIGRGTRLYDGKDYFTIYDFVKAHHHFADPEWDGEPLEPEPPKQ
                     KTAPQPEQPPTIREPREKYPKKDKIKVKLADGKERAIQHMMTTSFWHPDGTPMSAQQF
                     MELLFGKLPEFFKNEDELRRIWSAPETRKKLLQGLSEKGFGAEQLAEMQKIIDAEKSD
                     IFDVLAYVAYAMPTVRRDERAANARVKIHQTFSAKQQAFLEFVLQHYVSVGVEELDLE
                     KLTPLLQLKYHNSLADAVADLGRPEQINAIFSGFQKYLYIEAPVA"
     gene            complement(2751..2842)
                     /locus_tag="PKK36_08350"
     tRNA            complement(2751..2842)
                     /locus_tag="PKK36_08350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2806..2808),aa:Ser,seq:gct)
     gene            3057..4220
                     /locus_tag="PKK36_08355"
     CDS             3057..4220
                     /locus_tag="PKK36_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNR94603.1"
                     /translation="MTYGLSETQLEIRNMVREFALERVKPVRAELDEKEEFPTEILKE
                     LGRMDLMGLYIPEEYGGFGSDSMLDFIVAIEELSRICIGVSVSFAANALGADPILIGA
                     SDAQKKKYLPPLASGEKLAAFALTEPNAGSDAGGIQTTAELKGDKYILNGTKQWITNG
                     GEADIYTVLAVTDKKRGARGVSCFIVEKGMKGFSFGKKEKKLGIRASATRELIFEDCE
                     VPKENLIAREGMGFLLAMKTFDVSRPGIAAQGVGLAQGALDEAVNYARERVQFGRPII
                     ANQGMQWMLADMATKVEASRALLYSVCRTIDSGVKDVSKLSAMCKLFCTDTAMEVTVD
                     ALQVFGGYGYMREYPMEKMMRDAKILQIYEGTNQIQREVIGAALIKEYASKKI"
     gene            4266..5051
                     /locus_tag="PKK36_08360"
     CDS             4266..5051
                     /locus_tag="PKK36_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNR94604.1"
                     /translation="MHVVVCIKQVPDTSDVKINPETNTLIREGVQSIINPFDENALEI
                     ALVLKDTQGAKVTVITMGPPQAESALRECLARGADEVVLVSDRAFAGSDTLATSYTLA
                     RAIETLDKPDLILFGKQAIDGDTAQVGPGVAELLDMPVITYIRRVEVAEGKLKVERVF
                     EDGYEKMEVPLPAVLTILKEANVPRMASLKGRMKAKKAEIAMLSAAAIQADPERVGLK
                     GSPTKVVRIFTPPKKTGGIRIDGTHPLEAARTLVEKLREQKVI"
     gene            5062..6279
                     /locus_tag="PKK36_08365"
     CDS             5062..6279
                     /locus_tag="PKK36_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HNR94605.1"
                     /translation="MPLIIFEDKCTACTKCIKVCPFGALTMEPREKYDLPASRFKKIA
                     VVNEKCTACGACVQECPFEAMELREIKRTFKHNISDYKGVWIFAEQRGGHMEDVTYEL
                     LSKGRELADVRKVELSAVLLGSGVKEKASSLFAWGADNVYVVDDPMLEEYNTDLYTDV
                     VSTLISKYKPEMMLAGATSIGRAFIPRVAVRVQTGLTADCTALEIDSVRQILLQTRPA
                     FGGNIMATIECPDHRPQMATVRHKVLKKITPDESRTGKIIEEKMALKEPLSKIIEVLH
                     EMGNQVNLAEADIIVSGGRGLGGPENFKMIEELAKAIGGAVGASRAAVDADWIPAYHQ
                     VGQTGKTVCPKLYIACGISGAIQHLAGMSSSDFIVAINKDPDAPIFNVADIGIVGDLT
                     EIVPLITKALNDR"
     gene            complement(6307..7248)
                     /locus_tag="PKK36_08370"
     CDS             complement(6307..7248)
                     /locus_tag="PKK36_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR94606.1"
                     /translation="MPSKTLSVVIPLFNEENSIRELWQRLSAVMDALGIESEIIFVDD
                     GSGDQTGAELQKICQADPRVKLITLSRNFGHASALAAGLDHAQNQLVVLMDGDLQDKP
                     ESIPDFIAEWEKGFEVVYAVRTTRKENLLLRGAFKAFYRLLNRMSGIKQPLDAGIFCL
                     IDRKVVDVIRSMSERNRYFPGLRAYAGFRQTGIPVDRDARYAGKSRVRPIGLVKLALD
                     GLIAFSYIPLRMVTAAGVIVSFSAFAYMIRVLYKKLVSHEAILGWASTLTAILFLGGM
                     QLLTLGIVGEYIGRIYEEVKKRPYYIIKSIAAGKTGE"
     gene            7382..9445
                     /locus_tag="PKK36_08375"
     CDS             7382..9445
                     /locus_tag="PKK36_08375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR94607.1"
                     /translation="MKVDNQCKTGTGRFAEFLAGKRGMALLFLAAFVFRIAALGRASL
                     WQDEILFVQTSSPALHPFDVIKSYWDIVVSMAWMPLPAVLQNIYFHVAGSVIPDIAQK
                     PFWIRLPGTLWGSFAVLGVFALARRLLDRNAAPAAGIMTAVFFFPVFYAREAHAYPML
                     WFFAAFSLNQYFKLLGAQRSSLKDLIVFGVCLAGLGLTHLTGAMLLAALGASALLRWI
                     RLRFLVRNREGALHMLKTGFVCGLGMLAVAPYYLRVLTGSNPHLAGRQEMSPLFIAND
                     VVGKLFLGDRVWLVVLAWLVLLAGTVSLWIRGRQRAEARTALFVVGVTSVLLAAASWR
                     TQYLSARYFTAAAPLIYVLFAQGLHAAAQAGLRPFGREHYARGLLWIFLLILAGVHAC
                     VFLPRAYRLEEKHFDFAGIARWLNENARQGVPYVMESAYELRFVPGYYATPGLAAAAP
                     YVHGAGPDEIRRLRERQRAFLEQFPEAIFVETGHVGLHDMPELGPWTWLYGAYRQNVQ
                     LANSDLRALINMGIFPGYFRKDLLPVSFITDIRYNTREDILQIRRAAGLPAVFFFPDW
                     ACVPFERHPQGMWANYARYRSDLSGEIEAVSLAPAPVQGRILVHMAIDGDPSERFRVQ
                     MAVGNDILAESEAYAGRFFNLETGTLSLPPDGMKLVVRVEQKALTRFRGMLLRDAVFQ
                     PWSEK"
     gene            9442..>9747
                     /locus_tag="PKK36_08380"
     CDS             9442..>9747
                     /locus_tag="PKK36_08380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR94608.1"
                     /translation="MKAVKKYFITGGAGFIGAHRVNTILDSTDAQVTVYDNFSTGRRW
                     AYGDRISDSRLSIIEADVRDLPRLTEAMKGHDTVYHFAANSDIASAAKQPDVDFWNGT
                     "
BASE COUNT         2086 a         2723 c         2944 g         1994 t
ORIGIN      
        1 gctctcaagg ccgattacgt tctggtgtac cgcaacagga agctggcggt ggttgaggcg
       61 aaggcgtggg acgaggagtt gaccgagggc gttggtcagg ccaaggatta cgcggataaa
      121 ctaaaaatcc gcttcgccta ttccacaaac gggcagggca tctacggcat agacatgaag
      181 cccggcaagg aaggcgcggt ttccgcctat ccgtcaccgg aagagctctg gcagatgact
      241 tttgccgagc agaatgcttg gcgcgaccgg ttttccacgg ttccatttga agacaagggc
      301 ggaagccatc cgtcccgcta ttatcaggat accgccattg agaatgtgat ggaggccatt
      361 gctgcgggga agaatcgcat tcttctgacg ctcgccacgg gaacgggcaa gacctttatc
      421 gctttccaga ttgcgtggaa gctgttccac agccgctgga atctttcggg tgagccgaca
      481 cgccggcccc gcatcctgtt ccttgcggac cggaatattc ttgcgaatca ggcgtacaat
      541 gcgttttccg cgtttccgga ggatgctctg gtgcggattg ccccggacag catacgcaag
      601 aagggcaggg tcccgacgaa tggcagcctg ttctttacga ttttccagac gtttatgtcc
      661 gggcagtctg aagatggaga accaagccca tatttcgggg aatacccgaa ggactttttc
      721 gatttcattg tgattgatga gtgccatcgc ggcggggcca acgacgagag caactggcgg
      781 gatatcatgg agtatttctc gccagcggtt cagcttgggc tcacggccac cccgaaacga
      841 acagagaatg tggacaccta cagatatttc ggggagccgg tctacattta ctccctcaag
      901 gacggcatca atgacggctt tctgaccccc ttcaaggtta ggcagatcca gaccacactg
      961 gatgaatacg tatacacgcc tgatgacagt gtagtggagg gggagattga gagccggaag
     1021 acctatacgg agtccgattt caacaagatc attgagatca aggagcggga gaagaagcgc
     1081 gtccagattt tcatggagca gatcaaccag aatgagaaga cgatcgtgtt ctgcgccaca
     1141 caggaccatg cccttgcagt ccgggatctc atcaaccaga tgaagaccag caaggatccc
     1201 aactactgcc agagggtgac ggcgaatgac ggagagctgg gggagcagca tctccgtgcc
     1261 ttccaggaca acgaaaagac catcccgact atcctgacca cctcccagaa gctctcaacc
     1321 ggtgtggacg cccgcaatat ccgcaacatt gtcctgatgc ggcctattaa tcagatcatt
     1381 gagttcaagc agatcattgg ccgcggaaca cgactgtatg acggtaagga ttacttcacg
     1441 atctatgact ttgtgaaggc gcatcatcac tttgccgatc cggagtggga tggggagcct
     1501 ttggaaccag agccgccaaa gcagaaaact gcacctcagc ccgaacagcc accgacaatc
     1561 cgcgagcctc gggagaaata tcccaagaag gataagatca aggtgaagct ggctgatgga
     1621 aaagaacggg ccattcagca catgatgacc accagtttct ggcatccgga cggcaccccg
     1681 atgtcggccc agcagttcat ggagctgctc tttggaaaac tccctgagtt cttcaaaaac
     1741 gaagacgaat tgcgcagaat ctggagcgca ccagagaccc ggaagaaact gctgcagggg
     1801 ttgtccgaaa agggattcgg ggctgagcag ttggcggaga tgcagaagat catagatgcc
     1861 gaaaagagcg atatcttcga cgtgctggcc tatgtggcct atgcgatgcc aaccgttcgt
     1921 cgggatgagc gcgcagccaa tgcccgggtg aaaatccacc agaccttcag cgccaagcag
     1981 caggcgttcc ttgaattcgt cctgcagcac tacgtcagcg tgggggtgga ggaacttgat
     2041 ctggagaaac tgacgcccct ccttcagctc aaatatcaca actcattggc cgatgctgta
     2101 gccgacctcg gccgtcccga acaaatcaac gccatcttct ccggtttcca gaaatatctg
     2161 tacatcgaag ctcctgttgc atgacatcgg cccggcgggg aaggatctgg ggaaattaca
     2221 agaagacagt agtcagtagt cagaattcat tctttgcgtt ctctgtgctc tttcgcggta
     2281 aatattcttt tctgaaatct tgtgccttct gtgccttttc gtggctgaaa atctggaact
     2341 catgaactca ggaaattagc cgcaaaaatg cccaaaaatc gcaaaaaaac gcgaaaaatg
     2401 gccccaaaac ggcccaaaat gcccaaaaaa cgcgaaaaac agcccaaaat cgccccaaaa
     2461 cacgaaatag gaccaattta gtcctaattg catgatcttc ttttcttcat ggtgaaaaat
     2521 gttctccgaa cctccgcgcc tcagtgtctc cgcctgcggc ggacaagcct gcgcgaaaac
     2581 tcttcatttc tgcacttcag agccgtgcga aagcgcggtc aagcccgcgc actccaaagc
     2641 cgctttgctc cctgatgcgc ttcgcgatca gggcagtttc gttctcctga cggagaactc
     2701 agcgcgaacc ctccgggttc tcatcccggg agaacggcgg cgaatcagca tggcggagag
     2761 gccgggattc gaacccgggg taccagttgc ccggtacaac agattagcaa tctgccgctt
     2821 tcagccactc agccacctct ccgctgaagc agaatgtccg ggcaagatat acgcagaggc
     2881 gcatgcctgc aagttccttt tcacgcccgc ggccgtcccg gcccaaactt atcgggacgg
     2941 gccgcggatc aaaccttgac gaacaggggc cgtcttacta acttctgtct ctgtttcaat
     3001 gaggattgca catgggaatt ttagtaaccc tgtaacggga gattgttatg tctgatatta
     3061 cgtatggttt gagcgagacg cagcttgaga ttcgcaatat ggttcgtgaa ttcgccctcg
     3121 agcgggtgaa acccgtccgc gccgagcttg acgaaaaaga ggagttcccg accgaaattc
     3181 tcaaggaact gggccgcatg gatctgatgg gcctctatat ccccgaggaa tacggcggct
     3241 tcggatccga cagcatgctg gattttattg tggccattga agagctcagc cggatatgca
     3301 tcggtgtttc agtttccttt gccgccaacg cgctgggcgc ggacccgatc ctgatcggcg
     3361 cctcggacgc ccagaagaag aaatatctgc ccccgctggc ctccggcgag aaactggccg
     3421 ctttcgccct gacggagccc aatgccggca gcgacgcggg cggaatccag accaccgcgg
     3481 agctgaaggg cgacaagtac atcctgaacg ggaccaagca gtggatcacc aacggcggcg
     3541 aggccgacat ctacacggtg ctggctgtga ccgacaagaa gcgcggcgcg cgcggcgtgt
     3601 cctgctttat cgtggagaag gggatgaagg gcttcagctt cggcaagaag gagaagaagc
     3661 tcggcattcg cgcgtcggcg acccgcgaac tgattttcga ggactgcgag gttccgaagg
     3721 agaacctgat cgcccgcgaa ggcatgggct tcctgctggc gatgaagacg tttgatgttt
     3781 cccgccccgg cattgcggcg cagggcgtgg gccttgcgca gggcgcgctg gatgaggccg
     3841 tgaactatgc ccgcgagcgc gtgcagttcg gccggccgat catcgcgaac cagggcatgc
     3901 agtggatgct ggccgacatg gccacgaagg tcgaggcctc ccgcgcgctg ctctattccg
     3961 tctgccgcac cattgacagc ggcgtgaagg atgtctccaa gctctcggcc atgtgcaagc
     4021 tgttctgcac ggacacggcc atggaggtca cggtggacgc gctgcaggtg ttcggcggat
     4081 acggctacat gcgcgaatat ccgatggaga agatgatgcg cgacgccaag attctccaga
     4141 tttacgaggg caccaaccag atccagcgcg aggtgattgg cgccgcgctg atcaaggaat
     4201 acgcgtcaaa gaaaatctaa ccgctctttc ggcggcgctc aatctacaga gaaaggggca
     4261 cagggatgca tgttgttgtc tgcataaagc aggttcctga caccagcgat gtcaagatca
     4321 acccggaaac caatacgctg atccgcgagg gcgttcagag cattatcaac ccgttcgacg
     4381 agaacgcgct tgaaattgcc ctggtgctca aggatacgca gggcgccaag gtgacggtga
     4441 tcaccatggg cccgccccag gcggagagcg cgctgcgcga atgcctcgcg cgcggcgcgg
     4501 atgaggtggt gctggtgagc gaccgggcct ttgccgggtc ggacacgctg gccacttcct
     4561 acaccctggc ccgggccatc gaaacgctgg acaagccgga cctgattctc ttcggcaagc
     4621 aggccattga cggcgacacc gcacaggtgg gtccgggcgt ggctgaactg ctggatatgc
     4681 ccgtcatcac ctacatccgc cgcgtggaag tggcggaggg caaactgaag gttgagcggg
     4741 tgtttgagga cggatacgag aaaatggagg ttcccctgcc ggccgtgctg acgatcctga
     4801 aggaggccaa tgttccccgg atggcctcgc tgaagggccg catgaaggcc aagaaggcgg
     4861 agattgcgat gctgtccgcg gcggcgatcc aggcggaccc cgagcgggtc gggctgaagg
     4921 gttcgcccac caaggtggtg cgtattttca cgccgccgaa gaaaaccgga ggcatccgga
     4981 ttgacggcac gcatccgctg gaggcggcgc gaacactggt tgaaaaactc agagaacaga
     5041 aggtgatttg acgtgagcga catacccctg attatattcg aggacaagtg cacggcctgc
     5101 accaagtgca tcaaggtttg ccccttcggc gcgctgacca tggagccgcg cgagaaatac
     5161 gacctgcccg ccagccgctt caagaagatt gcggtggtca acgagaagtg caccgcgtgc
     5221 ggcgcctgcg tgcaggaatg cccgttcgag gcgatggagc tgcgcgagat caagcggacg
     5281 ttcaagcaca acatctccga ttacaagggc gtctggatct tcgccgagca gcgcggcggg
     5341 cacatggagg atgtcaccta cgagctgctc tccaagggcc gtgagctggc cgatgtccgc
     5401 aaggtggaac tctccgccgt cctgctggga agcggagtca aagagaaggc ctccagtctt
     5461 tttgcctggg gcgctgacaa tgtgtatgtc gtggatgatc ccatgctgga ggaatacaac
     5521 accgatcttt atacggatgt ggtttccacg ctgatcagca aatacaagcc ggagatgatg
     5581 ctggccggcg caaccagcat cggccgtgct tttatcccgc gcgtggcggt gcgtgtgcag
     5641 accggcctca cggccgactg cacggcgctg gagattgatt ccgtgcgcca gatactgctg
     5701 cagacgcgcc ccgcattcgg cggcaacatc atggccacca tcgagtgccc ggaccaccgc
     5761 ccgcagatgg cgacagtgcg gcacaaggtg ctgaaaaaaa tcacgcccga cgaatcccgc
     5821 acgggaaaaa tcatcgaaga aaaaatggcg ctcaaggagc cgctttcaaa gatcatcgaa
     5881 gtgctgcatg aaatgggcaa tcaggtgaat ctggcggagg cggatatcat cgtgtccggc
     5941 ggccgcggac tgggcgggcc cgagaacttc aagatgattg aagaactggc caaggccatc
     6001 ggcggggcgg tgggcgcttc gcgcgcggcg gtggatgccg actggattcc ggcctatcat
     6061 caggtcggac agaccgggaa aaccgtgtgc ccgaagctct atatcgcctg cggaatatcc
     6121 ggggccatcc agcatctggc gggcatgagc tcatccgatt tcatcgtggc gatcaacaag
     6181 gatcccgacg cgccgatctt caatgtggcg gacatcggaa tcgtgggcga cctcacggaa
     6241 atcgtcccgc tcatcaccaa ggccctgaac gaccggtgac gcggccggcc tccgggcgga
     6301 gatgccttac tcgccggttt tgccggcggc aatgctcttg atgatgtaat agggccgttt
     6361 cttcacttct tcgtagatgc ggccgatata ctcgccgacg atgcccagcg tgagcagctg
     6421 catgcccccc agaaagagaa tggcggtaag cgtggaggcc cagccgagga ttgcttcatg
     6481 tgaaacgagt ttcttgtaga gcacgcggat catgtaggcg aaggccgaga aagacacgat
     6541 gacccccgcg gcggtgacca tgcgcagggg gatgtacgaa aaggcgatga gaccgtccag
     6601 cgcgagcttg accagtccaa tcgggcggac gcggctcttg cccgcatacc gggcatcgcg
     6661 gtccacggga attcccgttt gccggaagcc ggcatatgcc cgcagcccgg gaaaatagcg
     6721 gtttctttcc gacatggacc tgatcacatc aacgaccttc cggtcaatca ggcagaaaat
     6781 gccggcatcc agaggctgct tgattcccga catgcggttg agaagccggt agaaggcctt
     6841 gaaggcgccg cgcagcagca gattttcctt ccgggtggtg cgcacggcat aaaccacctc
     6901 aaagcccttt tcccattccg cgatgaaatc cgggatggat tccggcttgt cctgcaggtc
     6961 gccgtccatc agaaccacca gctggttctg cgcatgatca agtccggcgg ccagcgccga
     7021 ggcatggccg aaattgcggg acagggtgat cagtttgacc cgcggatcgg cctggcaaat
     7081 tttctgaagt tccgctccgg tctggtctcc ggatccgtca tccacaaaaa tgatctcgga
     7141 ttcgatgccc agggcatcca tcacggcgga cagcctttgc cacagctcgc ggatgctgtt
     7201 ttcctcgttg aacaggggaa tgactaccga aagtgttttg gacggcatgc tgatcacctt
     7261 ctatatggag ctgctggttt taacatcaca catggctttt acttgccttg cgccggtgtg
     7321 tcaaatgtgt acaatgccga ttttcgattt gtcggacggc ttattggcgc ggctgaggcg
     7381 gatgaaagtg gacaatcagt gtaaaacagg aaccggccgg ttcgcggaat ttctggcggg
     7441 gaagcgggga atggcgctgc tgtttcttgc cgctttcgtg ttcagaattg ccgccctggg
     7501 ccgggccagt ctctggcagg atgagatcct gtttgtccag acttcaagtc ccgcccttca
     7561 tcccttcgat gtgatcaaat cctactggga cattgtcgta tccatggcgt ggatgccttt
     7621 gccggccgtc ctccagaaca tctattttca tgtcgcggga agcgtgattc cggacatcgc
     7681 gcagaaaccg ttctggatcc gcctgcccgg aacgctgtgg gggagttttg ccgttctggg
     7741 cgtttttgct ctcgcccggc ggctgcttga caggaacgcg gcgcctgcgg cgggaatcat
     7801 gacggcggtt tttttcttcc ccgtttttta tgcgcgcgag gcgcatgcct atcccatgct
     7861 ctggttcttt gccgcgttca gcctgaacca gtatttcaag ctgctggggg cacagagatc
     7921 gtcactgaaa gacctgattg tttttggcgt ctgtctggcc gggctcgggc tcacacattt
     7981 aaccggggcc atgcttctgg ccgcgctggg ggcctctgca ctgcttcgct ggattcgtct
     8041 tcgcttcctg gtccggaacc gggagggggc tcttcacatg ctgaaaaccg gttttgtttg
     8101 cggcctgggc atgctggcgg ttgccccgta ctatctccgt gttctcaccg gaagcaatcc
     8161 gcatctggcc ggccggcagg aaatgtcgcc cctgttcatt gctaatgatg tggtcgggaa
     8221 gctgttcctg ggcgacaggg tgtggcttgt ggtcctggcc tggctggtgc tgctggccgg
     8281 gacggtctcg ctctggatcc ggggccgcca gagggcggaa gcgcgcacgg ctctgtttgt
     8341 cgtgggcgtg acatccgttc ttctcgccgc ggcatcctgg cgcacgcagt atctgagcgc
     8401 ccggtatttt acggctgccg ccccgctgat ctatgtgctg tttgcgcagg ggctgcatgc
     8461 cgcggcgcaa gcgggactgc gtcccttcgg ccgggagcat tatgcccgcg gtcttctctg
     8521 gattttcctg ctgatccttg cgggcgtgca tgcgtgtgtc tttctcccgc gggcctatcg
     8581 gctggaggag aagcattttg attttgcggg catagcccga tggctgaatg aaaatgcccg
     8641 gcagggtgtt ccgtatgtca tggaaagcgc ctatgagctg cggttcgtcc cgggttatta
     8701 cgcaacgccc ggtctggcgg cggcggctcc gtacgtgcat ggagcgggtc ccgatgaaat
     8761 ccgcagactg cgcgagcgcc agcgggcatt tctggaacag ttccctgagg cgattttcgt
     8821 cgaaaccgga cacgtgggcc tgcatgacat gccggaactc ggtccgtgga catggctcta
     8881 tggcgcttac cggcagaatg tccagctggc gaacagcgac ctgcgcgctc tcatcaacat
     8941 gggcattttc cccggttatt tccggaagga tctgcttccc gtcagtttca taacggacat
     9001 ccgctacaat acccgcgaag atattctgca gatccgccgc gctgcgggtc tcccggcggt
     9061 gtttttcttc ccggactggg cctgcgtgcc ctttgagcgg catccgcagg gcatgtgggc
     9121 caactatgcc cgttatcgga gcgatctgtc aggcgagatc gaagcggtca gtcttgcgcc
     9181 ggcccccgtt caggggcgta ttcttgtcca tatggccatt gacggtgatc cgtcggaaag
     9241 attccgggtg cagatggccg tcggcaatga tattctcgcg gaaagtgagg cttatgccgg
     9301 ccggttcttc aacctggaaa ccgggaccct gtcactgcct cccgacggga tgaaactggt
     9361 tgtgcgtgtg gaacagaaag ccctgacccg gtttcgcggc atgcttctgc gggatgcggt
     9421 ttttcagcca tggagcgaaa aatgaaagcg gtaaaaaaat atttcatcac gggcggggcg
     9481 ggatttatcg gcgcgcacag ggtcaatacg attctggaca gcacggatgc acaggtcact
     9541 gtttacgaca atttcagcac gggccgccgc tgggcctacg gcgacaggat cagcgactcg
     9601 cgcctcagca tcatcgaggc cgatgtgcgt gatcttccgc gtctgaccga agccatgaaa
     9661 ggccatgaca cggtttatca cttcgccgca aattcggaca ttgcctccgc cgcaaaacag
     9721 ccggacgtgg atttctggaa cggaacc
//
LOCUS       DAOQWR010000099         9600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7598_length_9600_cov_5.121320, whole genome shotgun sequence.
ACCESSION   DAOQWR010000099 DAOQWR010000000
VERSION     DAOQWR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7598_length_9600_cov_5.121320"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PKK36_08385"
     CDS             complement(<1..796)
                     /locus_tag="PKK36_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNR94609.1"
                     /translation="MKKKVRFLEKTNGIIAMIHVRALPGTPRCEEDIPRIIDTAVEEA
                     AIYAREGIDMMMLENMHDVPYLNRSVGPEITASLTAVAQAVKREFPKKILGLQILAGA
                     NCEALAVAQAAQLDFIRAEGFVFAHTADEGIMNADAGPLLRYRRQIGAGNIKVFTDIK
                     KKHSSHTLTADVSIAETASAAEYFMSDGVIVTGKSTGCPADIGELRAVRDAVNIPVLI
                     GSGVTTDNVAGYFPLADALIVGSFFKKDGQWANAIDPARVARFMEKV"
     gene            922..2733
                     /gene="typA"
                     /locus_tag="PKK36_08390"
     CDS             922..2733
                     /gene="typA"
                     /locus_tag="PKK36_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNR94610.1"
                     /translation="MKRNIRNIGIVAHVDHGKTTLVDCLLRQSSTFRANEVIAERAMD
                     SMDLEREKGITIKSKNASITWHHTTINIVDTPGHADFGGEVERILKMVDGVLVLVDAV
                     EGPQAQTRFVLRKAIEQNLPLIFVVNKIDREHADPHGAHDKALELLLELNASEDQFHA
                     PFLYVSGRDGIAVRAIGDERVDMTPLFETVISHFPPPDASSRKPFEMLVSNLDWSDYV
                     GRIAVGKITGGHVNLGDSIVCIHKNGQRERQKITKIFGFVGMKSVELDSARAGDIVGI
                     AGFEDVFIGETLCDTEERDPLPFVEIDPPTIQMQLCANDGPLAGRDGRFVNARQIRER
                     LIRESRTNISISVEFSSTSSSFRVSARGELQIAILVETMRREGFELLVSRPEVIYHTE
                     SGRKLEPYENLWLEIPQDCLGDVLQSLAARRAETTSMQHHGNFVHIEAIIPTRGLIGL
                     ENYLVNRSSGQAVISHMFKEYAPVTGDIPLRSNGTLVSMAAGTSTAYALDTIQERGKL
                     FIGPGTEVYEGMIVGENARGEDLPVNPTRTKKLTNVRASGSDEAAMLAPPVIMTLEKA
                     IEFISNDEYVEATPNYLRLRKKILNSTERKRSGGRAS"
     gene            complement(2847..3254)
                     /gene="queF"
                     /locus_tag="PKK36_08395"
     CDS             complement(2847..3254)
                     /gene="queF"
                     /locus_tag="PKK36_08395"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNR94611.1"
                     /translation="MAKNEKIKGLTQLGTKSRQPQSPDEAKLETFDNRYPKSNYWIRF
                     QTSEFTSLCPITGQPDFATVTIEYIPGRKCIESKSLKLYLASFRNHGSFAESITNRIR
                     DDIAKACRPKKLKVTTDFTARGGITMRVTASKD"
     gene            3364..3780
                     /locus_tag="PKK36_08400"
     CDS             3364..3780
                     /locus_tag="PKK36_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNR94612.1"
                     /translation="MKSYRRELWFNTTKRRQLINITPEVETCLAESGIKEGLALVNAM
                     HITASVFINDDEGGLHQDYETWLEKLAPEKPYSQYKHNGYEDNADAHLKRTIIGREVV
                     VAVTNGSLDFGPWERIFYGEFDGKRRKRVLVKIIGE"
     gene            3836..4405
                     /locus_tag="PKK36_08405"
     CDS             3836..4405
                     /locus_tag="PKK36_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR94613.1"
                     /translation="MKEQPELEWLEQYRQGDVQALGLLVEHFRRPLFGFILRMTEGRG
                     DPEEVFQEVWFRAIRNLGKYKPKSFLSWLFRIAHNLIIDRARSARPMRSLNEAGEDET
                     PLEERLPGSTHDPAELTVAADLGQRIARAVGGLPVEQREVFLMRTEAGLPFKEIAKIQ
                     NISINTALARMQYALAKLREELKDDYHGS"
     gene            4409..4909
                     /locus_tag="PKK36_08410"
     CDS             4409..4909
                     /locus_tag="PKK36_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNR94614.1"
                     /translation="MKCDKAQNWILLDQTGELNGRQVKQLRDHLSGCAACRQFQGEAA
                     ELLGIKARVNPTPDHILHAIRLAGAREHVREKDSDGMLVFWRPLAAAAAALLLVVLSW
                     SMITGRDAPPVQLAKEWDNGIDEKLESLETMISSASYEIEYEYEWYEEDADELAALLL
                     YMEEST"
     gene            4915..5592
                     /locus_tag="PKK36_08415"
     CDS             4915..5592
                     /locus_tag="PKK36_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94615.1"
                     /translation="MTTAFLALITLSGTLGAQPYEPGPGMDSNDAGGLPPAERWMERM
                     RRHHPEEYQRMEHIRRENPAAFRGMLREKVDTKRVDAVMREYPPLNNAYQSLPPDKRA
                     GFIKKLYDRPRGPRGDDDPGRTRGRRPPADDEKPSSEIQQLDAEIMELGELYRDETNP
                     AVKKEIKERLEAKLHAMADTLDRHRTEKIAQMEKTLEELKQSLTERQKNREEIIARHL
                     VNLTGED"
     gene            5755..7680
                     /locus_tag="PKK36_08420"
     CDS             5755..7680
                     /locus_tag="PKK36_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNR94616.1"
                     /translation="MTALLLLLVSAAFLGGAGFYYSRFLARSWGEDAARKAPALELND
                     GRDYVPTPTPVVFAHHFASIAGAGPIVGPVLALCFGWGPALLWVVVGGLLIGGVHDYL
                     AAFMSIRHRGQSVAMIARRLLGRGPFLAFILFLVIMLGLVTAVFLNTSATALVSMLDL
                     GRMQLPANQTLFRTVEVGGQQQVIIGGVASMSVIVITCMAPLLGWLYIKKNVAVWKCS
                     LLAIGICAVSIFVGVHYPVTLSGAAWKITLAVYTLVAAGVPVWLFLQSRDFINVHILY
                     AGMIALFAALIVASIRGGSVFGPEAIPVADTVTGTAKLGGLWPALFITIACGAVSGFH
                     SLCAGGTTCKQLKSEGAARQIGYWGMILESLLAVIVIITLLVGTTKGFYLDDVFPAGK
                     DNNPVLGFAFAVGRVGHAAFGLPIAAGALAGMLLLEGFVITTLDTAIRLNRYLIEELW
                     LEFFGRFDVFAGRDSCIYAGTSAEITGGDGIPASMELPQENRLESVPTRGLLRLLLLT
                     LRQYWVNSGLAVALMLWLAFSGGILQLWKIFATSNQLLATFVLAIGSVWLIRNGKKVW
                     YVLLPALFMLATTGASLIILLRKFLPGRAADGTAFGNPTLFGADMVLLALTAYLLVAG
                     VMEIIRALRSRNPAPAA"
     gene            7918..9186
                     /locus_tag="PKK36_08425"
     CDS             7918..9186
                     /locus_tag="PKK36_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR94617.1"
                     /translation="MVEGKTVYESLHCTSREDAEKERKRIMRPLMAADKADVLANISK
                     RLTDAKDEQAAADEEANPPLAISAAWDAYYDSKSRPDSSERTLAGYESAWKRFKRWIE
                     KEHPDIKQMRDVSKNLAAAYATILEADGVTASTFNQHIGLMRLVWRTLAEEIRAGSNP
                     WMDMRRKKLGAQKVAHRRRNLTPEQFDSLLKCAGSADMHDLLFTLGWTGQRLADIVLL
                     KWNSIDFKRKVVTIHPLKLRRTGKEVHIPLLPQLADILKMRKRNAAGPHVFPELVEDY
                     QRDPSALSKRIAAAFDKAELPPREKLPGVSRAVVVYGAHSLRHYFATQALAAGIPAEI
                     VKRITGHTSDTMLANYEHLDARFIGELANKLQAQPLNPQPQIAALPAHEDNPLPPWAA
                     KMIEGMTAKNWKKVKTELLKTHAEPQEAGQ"
     gene            9183..>9600
                     /locus_tag="PKK36_08430"
     CDS             9183..>9600
                     /locus_tag="PKK36_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94618.1"
                     /translation="MKKKSSQNKNHKPKNISLSLDCFGLSNAVYPYRGQVAEGEKESK
                     PLPKSQAKAARDAISKAKNTIRAAHSVLLKYAGSTDYDIAYTAMLGLLNETLAGAEYA
                     SGLRGVNAPLLKIIAGHSYHWPVMVGIDPESWKAARE"
BASE COUNT         2172 a         2721 c         2771 g         1936 t
ORIGIN      
        1 tgactttctc catgaaccgg gcaacacgcg ccggatcaat ggcattggcc cattgcccgt
       61 ccttcttgaa aaatgatccc acgatcagcg catccgccag cgggaaataa ccggcgacat
      121 tgtcggtggt caccccggac ccgatcagca cgggaatatt caccgcgtcc ctaaccgcac
      181 ggagttctcc aatatccgcc ggacagccag ttgattttcc cgtcacaatg acgccgtcac
      241 tcataaaata ttccgcggcg gaggccgtct cggcgatgga tacatctgcc gtcagcgtat
      301 gagagctgtg ctttttcttg atgtcggtga acaccttgat gtttcccgcc ccaatctgcc
      361 ggcgatagcg gagaagcggc ccggcatcgg cgttcatgat gccctcatcg gccgtatgag
      421 caaaaacgaa gccctcggcg cggataaaat caagctgtgc ggcctgggcc acggccagcg
      481 cctcgcagtt ggccccggcc agaatctgca gtcccagtat ctttttgggg aactcccgct
      541 tcacggcctg cgccacggcc gtcagggagg ccgtgatttc gggcccgaca gagcggttga
      601 gatacggaac atcgtgcatg ttctccagca tcatcatgtc tatgccttcc cgggcataaa
      661 tcgcggcttc ctccacggcc gtgtcaatga tgcgtggaat gtcctcctcg caacggggcg
      721 tgccgggcag cgcccgcaca tgaatcatcg cgatgatgcc gttcgtcttt tccagaaacc
      781 gcactttctt tttcatgccc gcattgtatg ccgtattttt tcaaatacag caacaaatcg
      841 aaagattgaa ttacttcagc aattgcattg ccaacggccc ggaatccgct agagtctgcc
      901 cgctttactg agtcttcacc catgaaaaga aatataagaa atatcgggat tgtggcccat
      961 gtggaccacg ggaaaacaac gctggtggac tgcctgctgc ggcagtccag cacgttcaga
     1021 gcaaacgaag tcattgcgga acgggcgatg gattcgatgg atctcgagcg cgaaaagggc
     1081 atcaccatca agtccaagaa tgcgtccatc acctggcacc ataccaccat caacatcgtg
     1141 gacactcccg ggcatgccga tttcggaggc gaagtggagc gaatcctgaa gatggtggac
     1201 ggggtgctgg tgcttgtgga cgcagtcgaa ggcccccagg cccagacccg ttttgtcctg
     1261 cgcaaggcca tcgaacagaa tcttcctctc atctttgtgg tgaacaaaat cgaccgcgag
     1321 catgctgatc cgcacggcgc gcacgacaag gccctggagc tgctgctgga gctgaatgcc
     1381 tctgaggatc agtttcatgc cccgtttctt tatgtgagcg gccgcgatgg aatcgcggtt
     1441 cgcgcgatcg gcgatgagcg ggtggacatg acaccgctct tcgagacggt catcagccat
     1501 ttccccccgc ccgatgccag cagtcgaaag cccttcgaaa tgctcgtcag caaccttgac
     1561 tggagcgact atgtcggccg catcgctgtc ggaaaaatca ccggcgggca tgtgaatctg
     1621 ggtgattcca tcgtctgcat tcacaagaac gggcagcgcg agcgccagaa aatcacaaag
     1681 atattcggct tcgtcggcat gaaatccgtg gaacttgatt ccgccagggc cggtgacatc
     1741 gtgggcattg caggcttcga ggatgtattc atcggagaga ccctctgtga caccgaagaa
     1801 cgggatccgc ttccctttgt ggaaattgac ccccccacca ttcaaatgca gctctgtgcc
     1861 aacgatggtc cgctggcagg ccgcgacggc aggtttgtga acgcccgcca gattcgggag
     1921 aggctgattc gcgaatcgcg tacgaatatt tccatctccg ttgagttttc gtcaacctcc
     1981 agcagtttcc gcgtgagcgc ccgcggcgag cttcagatcg ccattctggt ggaaaccatg
     2041 cgtcgggaag gctttgaact gctggtgtcg cgcccggagg tgatctacca cacggagagc
     2101 ggccggaaac ttgaacccta tgagaatctc tggctggaaa tcccgcagga ctgccttggg
     2161 gatgtgctcc agtccctcgc cgcgcggcgg gcggaaacaa ccagcatgca gcaccacggg
     2221 aactttgttc acattgaggc catcattccc acccgcggcc tgatcggact tgagaactat
     2281 ctggtcaacc gcagcagcgg gcaggccgtg atcagccaca tgttcaagga atatgcgccg
     2341 gtcaccggcg atatccctct gcgcagcaac ggcaccctgg tctccatggc ggccggcaca
     2401 tcaaccgcct acgcgctgga cacaatccag gagcgcggaa agctattcat cggcccgggg
     2461 acagaagtgt atgaaggcat gatcgtcggc gagaacgcgc gaggggaaga ccttccggtc
     2521 aatcccacgc gcacgaagaa acttaccaac gttcgcgcat cgggctcgga tgaagcggcc
     2581 atgctggccc cgcccgtaat catgacgctg gaaaaagcca ttgagttcat ctccaatgat
     2641 gaatatgtgg aggccacgcc gaactatctt cgattgagga agaaaatact caactccacg
     2701 gagcgcaagc gctccggagg acgggccagc tagcatgtgc ccgccggccg cgccgaaacg
     2761 gggcggcccc cgcggccgaa tatttgatat gcctgttctt cccgtccagc agccgaaagc
     2821 ccgaaatcag cacattgccc gattcatcag tcttttgacg ccgtcacgcg catggtgatg
     2881 ccgccgcggg cggtgaaatc agtggtgact ttcagctttt tcggacggca ggcctttgcg
     2941 atgtcgtcgc gaatccggtt ggtgatggat tcggcaaaag acccgtggtt gcggaaggat
     3001 gcgagataca atttcagact cttgctttcg atgcatttgc ggccgggaat gtattcaatg
     3061 gtgaccgtcg cgaaatccgg ctgtccggta atcgggcaga gcgatgtgaa ttccgaggtc
     3121 tggaagcgga tccagtaatt gctcttcgga taacggttgt caaaagtctc cagcttcgcc
     3181 tcgtccggcg actgaggctg cctcgacttg gttcccagct gcgtcagacc cttgatcttt
     3241 tcattctttg ccatttgcct gtccttattg ttgcgacaga aacattgaac actcgcagca
     3301 aaaagtcaac gttgcgctgg atacgaaatc aaattagaat gccaaaaata accggagaac
     3361 attatgaagt cataccgcag agaactatgg ttcaacacga caaagcgcag gcagctcatc
     3421 aacatcacgc cggaggttga aacctgcctc gctgaaagcg gcattaagga aggtcttgca
     3481 ctcgtgaatg cgatgcacat caccgccagc gttttcatta acgatgacga aggcggtctg
     3541 catcaggatt atgagacatg gctcgagaag ctggcccccg aaaagcctta ttcccagtac
     3601 aagcacaacg gatacgagga caatgccgat gcacacctga agcgcacgat cattgggcgc
     3661 gaggtagtcg tggcggtgac gaacgggagc ctcgatttcg ggccgtggga gcggattttc
     3721 tacggcgagt tcgacggcaa gcgccgcaaa cgagtgctgg tcaagatcat cggcgaataa
     3781 ttcctgcaat atttccaatt cccgcccgtt atacgtgcaa aggaaaggcg aatacgtgaa
     3841 ggaacagccg gaactggagt ggctggagca gtatcggcag ggtgatgttc aggctctcgg
     3901 tctgcttgtg gaacacttcc gccggccgct tttcgggttc attctcagga tgaccgaggg
     3961 acggggcgat cccgaggagg tctttcagga agtctggttc cgggcgattc ggaatctggg
     4021 aaaatacaaa ccaaagagct tcctgagctg gctcttcagg atagcccaca atctgatcat
     4081 tgacagagca agaagtgcgc ggccgatgcg gagtctaaac gaggccggcg aggacgaaac
     4141 ccctctggag gaaagactgc ccgggagcac acacgatccc gcggaactga ccgtcgcggc
     4201 ggacctggga cagcgaatcg cccgcgcggt gggcggcctg ccggtagaac agcgcgaggt
     4261 gttcctgatg cggacagagg ccggcctgcc gttcaaggag atcgcgaaga tacagaatat
     4321 ctcgatcaac accgccctcg cgcgaatgca gtatgcgctg gccaaactga gagaagagct
     4381 gaaggacgat tatcacggga gctgagtcat gaaatgcgat aaggcacaga actggattct
     4441 gctggatcaa accggcgagc tgaacggacg gcaggtcaaa cagctgcggg atcatctctc
     4501 cggctgcgca gcctgtcgtc agtttcaagg cgaagctgct gaactgctcg gcatcaaagc
     4561 ccgtgtaaac cccaccccgg accatatcct gcacgccatc cggcttgccg gggcacgtga
     4621 acatgttcgg gagaaggatt cggacgggat gctggttttc tggcggccgc tggcggccgc
     4681 ggccgcggcg ctgctccttg tggtcctgtc ctggtccatg attacgggtc gcgatgcccc
     4741 ccccgttcaa ctggcaaagg aatgggacaa cggcattgac gaaaagcttg agagcctgga
     4801 aacgatgatc agcagcgcat catatgaaat cgaatacgag tacgaatggt atgaagagga
     4861 tgccgacgaa ctcgcggccc tgctcttgta tatggaggaa tcaacatgag aatcatgaca
     4921 actgccttcc tggcactgat cactctttcc ggaacgctgg gagcccagcc atatgaaccg
     4981 ggtcccggca tggattcaaa tgacgccggc ggcctgccgc cggcagagcg ctggatggag
     5041 cgcatgcgaa ggcatcatcc ggaagaatat cagcgcatgg agcatatccg ccgcgaaaac
     5101 ccggcggctt tccgcggcat gctgcgcgaa aaagtggata cgaagagagt ggatgccgtt
     5161 atgagagagt atcccccgct gaacaatgcc tatcagtctc tgccgcccga caagcgagcc
     5221 ggcttcataa agaaactcta tgaccggccc cggggacccc gcggcgatga tgaccctggc
     5281 cgcacaagag gccgccggcc gccggccgac gacgaaaagc cctcaagtga aatacagcag
     5341 cttgatgcgg aaatcatgga gcttggcgaa ctctaccggg acgagacgaa tccggcagtg
     5401 aaaaaagaga tcaaagaaag gctggaagca aagcttcatg cgatggcaga taccctggac
     5461 cggcatcgca cggaaaaaat cgctcagatg gaaaagaccc tggaggaact taagcagagc
     5521 ctcactgaac gccagaagaa ccgcgaggaa ataatagccc ggcacctcgt aaatctgacc
     5581 ggggaagact gattcagcgg gagcctgccg ggccctttcc gcagaatcag gccggtttcc
     5641 agataattgc ccgcatgagg gatttccatg aaatttcctg ttgccctccg atcctcaacg
     5701 ggtctatatc ttgaccattt ggatgacctt tgaacaggtc gaaagagggt ggatatgaca
     5761 gcgctgcttt tgcttcttgt ttcggccgct ttccttggcg gagccggttt ctattattca
     5821 cgtttccttg cccgaagctg gggcgaagat gcggcccgca aggcgccggc attggaactc
     5881 aatgatggcc gagactatgt gcctaccccc accccggtgg tgttcgccca tcacttcgcg
     5941 tcgatcgccg gggcaggccc cattgtcggt ccggtgctgg ccctgtgttt cggctggggg
     6001 ccggctctcc tgtgggtggt tgtcggcggg cttctcattg gcggagtgca cgactatctc
     6061 gcggcgttca tgtccatccg ccatcgcgga cagtctgtgg ccatgattgc gcgccgcctg
     6121 ctgggccggg gccctttcct tgcctttatc ctgttcctcg tcatcatgct cggactggtc
     6181 acggccgttt ttctcaacac gtccgccacc gcgctcgtca gcatgctcga tctgggccga
     6241 atgcagcttc cggccaacca gacgctgttt cgaaccgtgg aagtcggcgg acagcagcag
     6301 gtcataatcg gcggcgtggc ctccatgagc gtgattgtca tcacctgcat ggccccgctc
     6361 ctggggtggc tgtatatcaa aaaaaatgtg gccgtatgga aatgttctct gctggccata
     6421 ggcatttgcg cggtcagcat atttgtgggc gtgcactatc cagtgaccct ctcaggtgca
     6481 gcctggaaga ttacactggc ggtttacact ctggtcgccg caggtgttcc tgtctggctt
     6541 ttcctacaga gccgcgattt catcaatgtc cacatcctgt atgccgggat gatcgcactt
     6601 tttgccgccc tgattgtggc ctccatccgc ggcggctcag ttttcggccc ggaagctatc
     6661 cccgttgcgg acaccgtgac cggcaccgcc aagctgggcg ggctctggcc ggccctgttc
     6721 attaccattg cctgcggcgc cgtgagcgga tttcacagcc tctgcgccgg aggaaccacc
     6781 tgcaagcagc ttaagagcga aggtgccgcg cggcagatcg gctactgggg catgattctt
     6841 gaaagcctcc tggccgtcat cgtgattatc accctgctgg tgggaaccac caagggtttc
     6901 tatctcgatg atgtatttcc ggccgggaaa gacaacaacc cggtactggg tttcgctttt
     6961 gctgtcggcc gggtcgggca cgccgcattc ggccttccca tcgcagcggg cgcgctggcg
     7021 ggcatgctgc ttctggaagg atttgtcatc accacgctgg atacagccat ccgcctgaac
     7081 cgctatctga tcgaagagct ctggctggag ttctttggcc gctttgatgt tttcgccggg
     7141 cgggattcct gcatctatgc cggaacgtct gctgaaatca caggaggcga cggaatccct
     7201 gccagcatgg aactgcccca ggaaaaccgt ttggaatccg ttccaacccg cgggctgctg
     7261 cgcctgttac tgctgacact ccggcaatac tgggttaatt ccgggctcgc ggtggccctg
     7321 atgctctggc tggccttcag cggcggcatt ctacagctct ggaagatatt tgccaccagc
     7381 aatcagcttc tggccacgtt cgtgctggcc atcggttccg tgtggctcat ccgcaacggg
     7441 aaaaaggtct ggtatgtgct gctgcccgcg ctcttcatgc tggccaccac cggagcaagc
     7501 ctgatcattc ttctgcggaa attccttccc ggccgggcag cggacggcac cgctttcgga
     7561 aatccgactc tgttcggtgc cgacatggta ctgctggcac tgacggccta tctgctggtg
     7621 gccggagtca tggaaatcat ccgcgctctg cggagccgaa atccggcgcc cgccgcataa
     7681 aggaaaaact ggtagaggcg cagggactcg aaccctggac ccgctgatta agagtcaata
     7741 gcatgtgtgc attttatagg ctatttccga ttgactgtct caaaacttgg tgtaatgtct
     7801 ggggatatgt agtcatataa ggcaatggaa cgcaatagag gaaaggcggc taaaatgaca
     7861 cggaaacacc ggacgggaaa cttatacagg cggggaaatg tttggtggct caggtacatg
     7921 gtggagggca aaaccgttta tgaatccctg cactgcacca gccgggagga tgccgagaag
     7981 gagcgcaagc ggatcatgcg ccccctgatg gccgccgaca aagccgacgt gctggccaat
     8041 atctcaaagc gactgaccga cgcgaaggac gaacaggccg ccgccgacga ggaagccaat
     8101 cccccgctgg caatctccgc cgcttgggat gcgtactatg acagcaagag ccgtcccgat
     8161 tcatcggaga gaacgctggc cggatatgag agcgcatgga aaagattcaa gcggtggatc
     8221 gagaaggaac acccggacat caaacagatg cgcgatgttt caaagaacct tgccgccgct
     8281 tacgcaacaa tacttgaagc ggatggagtg accgccagca cgttcaacca gcatatcggg
     8341 ctgatgcgtc tcgtatggcg tacactggca gaggagattc gcgcaggttc caacccgtgg
     8401 atggacatgc gccggaagaa actcggagcg cagaaggtgg cgcaccgccg ccgcaatctg
     8461 acacctgagc aatttgattc ccttctgaaa tgcgcgggca gtgcagacat gcacgacctt
     8521 ctttttactc ttggatggac tggccagcgc ctcgcggaca ttgttctatt gaagtggaac
     8581 tccatagatt tcaaacggaa ggttgtcacg attcacccgc tgaaattgcg gcgcacaggc
     8641 aaggaggttc atattccgtt gctgccccag cttgcggata ttctcaaaat gagaaagcgg
     8701 aacgccgccg ggcctcatgt gttccccgaa cttgtggaag attatcagcg tgatccgtca
     8761 gcactgagca agcggattgc cgccgccttt gacaaggccg aactgccccc ccgcgaaaag
     8821 ctgcccggag tatctagggc cgttgtcgta tatggagcgc atagtctgag gcactacttc
     8881 gcaacgcaag cccttgccgc cgggataccc gccgagatcg tgaagcggat caccggacac
     8941 accagcgaca ccatgcttgc caactacgag catttagatg cgcgatttat cggagaactg
     9001 gcgaacaagc tacaggctca accactgaac ccgcagccgc aaatcgccgc actgccagcg
     9061 catgaggata atcccctgcc cccgtgggcc gcgaaaatga tcgagggaat gacagcaaag
     9121 aactggaaga aagtgaaaac cgaactgctc aagactcatg cggaaccaca ggaggccgga
     9181 caatgaaaaa gaaatcctca cagaacaaga accacaagcc caaaaatatc agcctgtctc
     9241 ttgactgctt cgggctatca aacgcggtgt atccataccg tggacaagtt gcggagggcg
     9301 aaaaggaatc aaagccattg cccaagagtc aggcgaaggc ggcgcgtgat gccatctcga
     9361 aggcaaagaa caccatcagg gcggcgcaca gcgtcttgct gaaatatgcc gggagcacag
     9421 attacgacat tgcctatact gcaatgcttg gtctgctgaa tgaaactctt gctggcgctg
     9481 agtacgcttc tgggcttcgc ggagtgaatg cgccactgct caagatcatt gccgggcatt
     9541 cgtatcactg gccagttatg gttggcattg atcccgaaag ctggaaggcc gcgagggaac
//
LOCUS       DAOQWR010000100         9529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7677_length_9529_cov_5.536838, whole genome shotgun sequence.
ACCESSION   DAOQWR010000100 DAOQWR010000000
VERSION     DAOQWR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_9529_cov_5.536838"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PKK36_08435"
     CDS             complement(<1..1519)
                     /locus_tag="PKK36_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNR94619.1"
                     /translation="MRECPAKAIRILEGQAQIISERCIGCGNCVRVCSQGAKQIVNTI
                     YQAQALLDSDHPVSALVAPSFAAEFGEDLARHVVAAIRMLGFNHVFEVGFGADLVAHR
                     YQQLLLQDTGRRCIATSCPAIVLYVEKHHPSLVSSLAPVVSPMIAMARVVRKIHGGAM
                     RTVFIGPCTSKKFEGVDEDVCGEINAVLTFRELRQMFDGAGIVLEKTEPCDFEPPHAG
                     LGALFALCSGLLQAAGINEDLVTGDVVTAAGRRGFLEAMKEFESGDLDARLLESLACD
                     GCIMGAGMSVDLPAFRRRSRVSRYVQRRRRELNLQQWQASMEWFGTLDLTRNYRSRRV
                     PVTPPDPERVREVLRRMGKEGPEDLLDCGACGYETCHAHAVAICEGLAESEMCLPYTI
                     DRLEQAVNDLAQSNEQLLTTRDALMQSEKLASMGQLAAGIAHEVNNPLGVVLMYAHLL
                     KDSFEKDSPAWRDSVMIAEQADRCKRIVAGLLNFARQNKVLRQPVDVRHLIGQSIK"
     gene            complement(1585..3306)
                     /locus_tag="PKK36_08440"
     CDS             complement(1585..3306)
                     /locus_tag="PKK36_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNR94620.1"
                     /translation="MITIEVNGRPIAAKKGEMLIAALDRAGIKVPTLCYMKDRFPTGA
                     CRMCVVEIEGQKGLIPSCAYPVAEGMKVQTHSPRAVNARKTIVELLLADHPDDCLYCV
                     RNGNCDLQKMAEDLGLRRRRYAGEKSVWKMDLSSPAIERDPDKCILCGKCVRTCEEVQ
                     GVSAISFAGRGCRTFIGTAFNEGMNISSCVYCGQCIKVCPTGALRERSSIKEVWGALN
                     DPEMTVVVQHAPSVSVTVGDAFGVKPGVDMAGAMTTALRRLGFDYVFDTSFSADLTIM
                     EEASELVQRIKTGGKLPMMTSCSPGWIKYVEQNYPEFMGNLSTCKSPQQMMGAVIKSY
                     FAEKMGLSPKKIFSVSIMPCTAKKFEATRSEMGTRSIPDIDAVLTTREFIRMIRMSGL
                     DLNDLEPDTADTPFGERSTAGKLFGASGGVMEAAVRTAHYLLTGRELKVMALEAVRGM
                     KDRKEAQLEINGMKIGIAVVNGLGGAKPLLDEIRAGRDDLHFIEVMTCPGGCINGGGQ
                     PIDMNMDAVKARMAALYQLDAKARLRTSHSNPSIMRIYKEFLGDPLGPVSHRLLHTKY
                     MERKVLN"
     gene            complement(3312..5297)
                     /locus_tag="PKK36_08445"
     CDS             complement(3312..5297)
                     /locus_tag="PKK36_08445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNR94621.1"
                     /translation="MSIATTPCCGKCWHTPDTPCPDIAVCLQEGPQCHDDAVCRTAVK
                     ERLAAARRDHVERPVIFLGMGTCGLGAGAARTREAVMSFLESKKIAADIVETGCVGMC
                     SAEPLMDVQIPGRSRICFKRVTEKNAAAILESVLKGAVPEGSALGQYRGGQAWADVPV
                     LWESSFFRNQPRRVLKNCGIIDPGSMDEYIAHGGYRAMGMMLKNMTPAEVCDVVEQSG
                     LRGRGGGGFLTGRKWKFALNTVSDQKYLICNADEGDPGAFMDRAVIESDPHRLIEGMV
                     AAAYGIGATKAYVYIRAEYPLAIERLIRAIAQAKEAGFLGYNIFDSGFNLDITIKKGA
                     GAFVCGEETALIHSIEGKRGMPRVRPPYPAVRGLFDRPTIINNVETLANLPELMEKGA
                     AWFSSIGTKTSAGTKVFALSGNVRNTGLVEVAMGTKLRDIVFEIGGGIEGDRRFKAVQ
                     IGGPSGGCIPEHLLDIDIDYESLKTVGAMMGSGGLVVMDERTCMVDLAKFFMDFIQRE
                     SCGKCIPCREGTRRMLETLRRITRGRKSEKDIDALDRFQSVVYMERLAKVIGDTSLCG
                     LGQTASNPVLSTLRYFRDEYEAHVYDRRCPAGVCSELVTYRIDAAACKGCTQCAKKCP
                     SGAIMGARKAPHYIIPDKCIACGACFDVCPFDAVRKE"
     gene            complement(5294..5773)
                     /gene="nuoE"
                     /locus_tag="PKK36_08450"
     CDS             complement(5294..5773)
                     /gene="nuoE"
                     /locus_tag="PKK36_08450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNR94622.1"
                     /translation="MSRAMDSILKKYRRAGIDSLIPMLQDVQAEQGYLSKDAVAEIGR
                     HLKVPASKIYGVATFYNQFRFEKLGRYHIQICRGTACHVKGSATLQKVLEQQLGVKAG
                     QTTRDGLFSLEVVACIGACGLAPVISVNGEFHASVKQEEVGKILDVYRRRAESEGGV"
     gene            complement(5770..6408)
                     /locus_tag="PKK36_08455"
     CDS             complement(5770..6408)
                     /locus_tag="PKK36_08455"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNR94623.1"
                     /translation="MISEKTIERLSQYRHFLREFLKENVDHVSSSTLAERCHGQSAQV
                     RRDLMEMGYRGKSTRGYDVEDLLAAIGSYLDRPGGEKVALCGIGNLGRALLTYFQGSL
                     SNLQVVAAFDRDPHKTGRVIHGCRCFDIEMVEKVIAEQGIQVAVIAVPGPDAQRVADR
                     LFAAGIKGLLNFAPTPVRVPPKTYVEHMDMTMALEKVSFFTRQLAPARGAPK"
     gene            6726..7109
                     /locus_tag="PKK36_08460"
     CDS             6726..7109
                     /locus_tag="PKK36_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94624.1"
                     /translation="MAKILIVDDDPDVVEASSLVLAGAGYEVGTASSRAAGMEQIQSF
                     KPDALILDVMMDEPDDGIAMAQELRRTGFTRPILMLTSLGKVLGMDFGKDESVVPVDD
                     FVEKPINPGDLVARVKKLLAAKKGK"
     gene            7115..7636
                     /locus_tag="PKK36_08465"
     CDS             7115..7636
                     /locus_tag="PKK36_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNR94625.1"
                     /translation="MLTPERDQLAENITKWIDQYGGNRSALLPTLQEIQKRHGRVTEY
                     AMQVVADKLSIHPVEVYGVITFYAFLDTKYKGRFIIRLCRTISCDMAGKSRVARQLEN
                     DLGIKFGESTADGRFTLEWANCLGLCDHGPALLVNSQVFTHVTPEKVHDIIESCRKAF
                     GAHTLQKEEVHIA"
     gene            7633..8826
                     /locus_tag="PKK36_08470"
     CDS             7633..8826
                     /locus_tag="PKK36_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNR94626.1"
                     /translation="MSETYTAPLTFSKVKAGKGLEAALELDRAGVINEITASGLKGRG
                     GAGFPTGVKWNLAAAAKDPVKYVVCNADEGEPGTFKDRVILTDHADLVFEGMTICAYA
                     IGAKKGILYLRGEYTYLQDHIEGILRKRREAKLLGKNAGGKKGFEFNIEVRMGSGAYI
                     CGEETALIESLEGQRGEPRNRPPFPVNTGFLGHPTVPNNVETFAWAATIMSKGSEWFK
                     KIGTDKSAGLKIFSVSGDCKQPGVYEFPLGITIKELLKAVGGLDAKAVQVGGASGSCV
                     PAREFNRKIAFEDVATGGSIIVFGPQRDMLKVAENFMEFFHDESCGQCTPCREGNGKI
                     LEGIHMLQQGKCSMHYLRELCALGETMQLASKCGLGQTSPNAFLSIVKNFPDEIMGRA
                     GAAQE"
     gene            8846..>9529
                     /locus_tag="PKK36_08475"
     CDS             8846..>9529
                     /locus_tag="PKK36_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNR94627.1"
                     /translation="MTDKNIKLKEESRAPVSSSVQKFAAPQNEKAIGATITVTIDDKQ
                     IKVPMGTTILEAAKKLNIHIPTLCYHEDLCVAGVCRICAVEVEGQRTLQASCAYPITA
                     PITVRTHTRKVRQARRHILDLLLSKHYGECYACARNNNCELQALAKEYGVDFFRFGHP
                     DKPRFEVDRSSYSVVRDMDKCILCRRCVRTCIDLQEVGTIEVIERGAKSHIGTFLEKP
                     LGSVVCINCG"
BASE COUNT         1894 a         3042 c         2758 g         1835 t
ORIGIN      
        1 gcttgatgct ctgcccgatc aggtgccgca catccaccgg ctggcgcagg accttgttct
       61 ggcgggcgaa gttgagcaaa ccggccacga ttctcttgca gcggtccgcc tgctcggcga
      121 tcatgacgga gtcgcgccac gcggggctgt ctttctcgaa gctgtccttc agcagatgcg
      181 cgtacatcag caccacgccc agcggattgt tcacctcgtg ggcaatgccg gcggccagtt
      241 gccccatgct ggcgagtttt tccgactgca tcagggcgtc gcgggtggtc agcagctgct
      301 cattggattg cgccaggtca ttaacagcct gctccaggcg atcaatggtg tacggaagac
      361 acatctcgct ttcagccaga ccttcgcaga tcgccactgc gtgagcatgg caggtctcgt
      421 aaccgcaggc gccgcaatcg agcaggtcct caggaccctc cttgcccatc cggcgcagca
      481 cttcacggac gcgctcggga tcaggcggcg tcacgggaac ccgccgggaa cgataattcc
      541 gggtgagatc caatgtcccg aaccactcca tgctggcctg ccactgctgc agattcagct
      601 cgcggcggcg gcgctgcaca tagcggctta ccctcgatcg gcggcggaac gccggcagat
      661 ccacgctcat gccggccccc atgatgcagc cgtcgcaggc gaggctttcc agcagacgcg
      721 cgtcaaggtc gccgctctca aactccttca tggcctccaa aaaaccgcgc cggccggcgg
      781 ccgtcaccac atccccggta acaagatctt cgttgatgcc ggcggcctgc agcaaaccgc
      841 tgcaaagcgc gaagagggcg cccagcccgg cgtggggcgg ttcaaaatcg catggctccg
      901 tcttttccag aacaatgccc gcgccgtcga acatctgacg cagttcgcga aacgtcagca
      961 cggcgttgat ctctccgcag acatcttcgt ccacgccttc aaactttttt gatgtgcagg
     1021 ggccgatgaa cacggtgcgc atggccccgc cgtgaatctt gcgcacgacg cgggccatcg
     1081 caatcatcgg ggaaacaacc ggcgccagcg aggacacaag gcttggatgg tgcttttcga
     1141 catacagcac gatcgccggg cagcttgtcg cgatgcagcg ccggccggta tcctgcagga
     1201 ggagctgctg atagcggtga gccaccagat cggcgccaaa accgacttca aacacatgat
     1261 tgaagcccag catgcggatg gcggcaacaa cgtggcgggc aagatcctcg ccgaactcgg
     1321 ccgcaaaact cggcgccacg agcgccgaca ccggatgatc actgtccagc agcgcctggg
     1381 cctgatagat cgtgttgacg atctgctttg cgccctgcga gcagacccgc acacagttgc
     1441 cgcatccaat gcagcgctcg gagatgatct gcgcctgtcc ctccagaatg cgaatggctt
     1501 ttgccggaca ttcgcgcaca caggtgtagc agacccggca gcgctccggg atggttacaa
     1561 tcgccgcatt gttctccccg attttcagtt cagcaccttt ctttccatgt atttcgtgtg
     1621 cagcagcctg tggctgacag gccccagcgg gtccccaagg aattccttgt agatacgcat
     1681 aatggacggg tttgaatgcg aagtccgcag ccgggctttc gcatccagct ggtacagcgc
     1741 cgccatgcgg gccttcacgg catccatgtt catgtcaatc ggctggccgc cgccgttgat
     1801 gcatccgccg gggcaggtca tgacctcaat gaaatgcagg tcatcccggc cggcgcggat
     1861 ttcgtcgagc agaggcttcg ccccgccgag cccgttgacc acggcgattc cgatcttcat
     1921 gccgttgatt tccagctggg cctctttgcg gtctttcatc ccgcggacgg cttccagcgc
     1981 cataaccttc agctcgcggc cggtcagcag atagtgagcc gtgcgcacgg ccgcctccat
     2041 cacgccgccc gatgctccaa agagcttccc ggccgtgctg cgttcgccaa acggcgtatc
     2101 ggccgtatcg ggttcaagat cattcaagtc gagcccgctc atgcggatca tgcgaataaa
     2161 ctcgcgtgtg gtcagcacgg catcgatgtc cggaatgctg cgtgttccca tctcggagcg
     2221 cgtggcctcg aatttctttg ctgtgcaggg catgatcgag acgctgaaga ttttcttcgg
     2281 ggaaaggccc attttctcgg cgaagtagct cttgatcacc gcgcccatca tctgctgcgg
     2341 gctcttgcag gtggaaagat tgcccatgaa ttcgggataa ttctgctcca cgtatttgat
     2401 ccatcccggc gagcagctgg tcatcatcgg cagtttcccg ccggttttga tgcgctgcac
     2461 cagctccgag gcctcctcca tgatcgtaag gtcggcgctg aaggatgtgt cgaaaacata
     2521 gtcaaacccc agccgcctca gggcggtggt catggcgccc gccatgtcca ccccgggctt
     2581 gacgccaaag gcatcgccca cggtgaccga aacggaaggc gcgtgctgga ccaccacggt
     2641 catctccgga tcgttcagcg cgccccagac ttccttgatg gagctgcgct cgcgcagcgc
     2701 gccggtgggg cagaccttga tgcactgacc gcagtagacg cagctgctga tgttcatgcc
     2761 ctcgttgaag gccgtgccga tgaaggtcct gcagccgcgg cccgcgaagg atatggcgga
     2821 tacgccctgc acctcttcgc acgtgcgcac gcatttgccg cagagtatgc acttgtccgg
     2881 atcgcgctca atggcgggac tggaaagatc catcttccag acgcttttct ctcccgcgta
     2941 gcggcggcgg cgcaggccca ggtcctccgc catcttctgc aggtcgcagt tgccgttgcg
     3001 cacgcagtag aggcagtcgt ccggatggtc cgccagcagg agttcgacaa tggtcttgcg
     3061 ggcgttcacc gcgcgcggcg aatgcgtctg caccttcatg ccctcggcca cgggataggc
     3121 gcagctcggg atcaggccct tctggccttc tatttcgacc acgcacatgc ggcacgcgcc
     3181 ggtcgggaac cggtccttca tgtaacaaag ggtcgggacc ttgatgcccg cgcgatccag
     3241 cgcggcgatg agcatttcgc ccttcttcgc cgcgatcggc cttccgttga cttcaatggt
     3301 aatcatagcc gttactcctt gcgcaccgca tcgaacgggc acacatcaaa gcaggcgccg
     3361 caggcgatgc acttgtcggg gatgatgtaa tgcggggcct tccgcgcgcc catgatggcg
     3421 ccggaagggc acttcttggc gcactgcgtg cagcccttgc aggccgccgc atcaatgcgg
     3481 taggtcacga gctcgctgca gactcccgcg gggcagcgcc ggtcatagac gtgcgcctca
     3541 tactcgtcac gaaaatagcg cagcgtgctc agcaccggat tggaggccgt ctggcccagc
     3601 ccgcagaggc tggtgtcgcc gatgaccttg gccaggcgct ccatatagac cacgctctgg
     3661 aaacggtcca gcgcatcaat gtccttttcg ctctttcgac cgcgcgtgat gcgcctgagg
     3721 gtctccagca tgcggcgggt gccctcgcgg cagggaatgc acttgccgca gctctcgcgc
     3781 tggataaagt ccatgaagaa tttcgcaagg tccaccatgc aggtgcgttc gtccattacc
     3841 accaggccgc cggaacccat catggcgccc acggttttga gagattcata gtcaatgtca
     3901 atatccagca gatgctccgg gatgcagccg cccgaaggcc cgccaatctg cacggccttg
     3961 aacctgcggt cgccctcgat cccgcccccg atctcgaaaa caatgtcgcg cagtttcgtg
     4021 cccatcgcca cttcgaccag acccgtgttg cgcacattgc ccgagagcgc aaacaccttg
     4081 gtgccggcgc tggtttttgt cccgatggat gaaaaccatg cggcgccctt ctccatcagt
     4141 tccggcaggt tggccagggt ttccacattg ttgatgatcg tgggccggtc aaaaagtcct
     4201 ctgaccgccg gatacggcgg acgcacgcgc ggcatcccgc gtttgccctc gatgctgtga
     4261 atcagggccg tttcttcgcc gcagacaaag gcgcccgccc ccttcttgat tgtgatgtcc
     4321 aggttgaatc cgctgtcgaa tatgttgtag ccgagaaagc cggcctcctt cgcctgcgca
     4381 atcgcccgga tcagccgttc aatggccagc gggtattccg cgcgaatata gacataggcc
     4441 ttggtcgcgc cgatgccgta tgcggcagcc accatgccct cgatcaggcg gtggggatcg
     4501 ctttctatca cggcgcggtc catgaacgcg ccgggatcac cctcgtccgc gttgcagatc
     4561 aggtatttct ggtcgctcac ggtgttcagc gcgaacttcc acttccggcc cgtcagaaat
     4621 ccgccgccgc cccgtccgcg caggccgctt tgttcaacaa catcgcagac ttcagcgggc
     4681 gtcatgttct tgagcatcat gcccatggcc cggtatccgc cgtgcgcgat atattcgtcc
     4741 atgcttcccg gatctatgat cccgcagttc ttcagcaccc gccggggctg gttcctgaaa
     4801 aagctgctct cccaaagcac cggcacatca gcccatgcct gtccgccgcg atactgaccg
     4861 agcgccgagc cctcggggac ggcgcccttc agaacggatt caagaatggc cgcggcgttc
     4921 ttctccgtga cccgtttgaa acagatgcgg gagcgcccgg gaatctgcac atccatcagc
     4981 ggctcggccg agcacatgcc cacgcagccc gtctcaacaa tgtccgccgc gatctttttg
     5041 gactccagga atgacatcac ggcctcgcgg gtccgtgccg cgcctgcccc caggccgcag
     5101 gttcccatcc cgaggaaaat gaccgggcgc tccacatgat ccctgcgggc ggccgcaagg
     5161 cgctctttca cggcggttct gcaaaccgca tcgtcatgac actgcggccc ctcctggagg
     5221 cagactgcga tgtcgggaca gggcgtgtcc ggggtgtgcc agcacttgcc gcagcagggt
     5281 gtggttgcga tgctcatact ccgccctcgc tttccgcccg ccggcggtag acatccagga
     5341 ttttgccgac ttcctcctgc ttcacgctgg catgaaattc gccgttcacg ctgatcacgg
     5401 gggcaagacc gcaggcgccg atgcacgcca ccacttcaag gctgaacaga ccgtcacgcg
     5461 tggtctgccc cgccttgacg ccaagctgct gctccaggac cttctggagt gtggcggatc
     5521 ctttcacgtg gcatgccgtt ccgcggcaga tctggatgtg atagcggccc agtttctcga
     5581 agcggaactg gttgtaaaat gtggccacgc cgtagatttt gctggccgga accttcaaat
     5641 gccggccgat ctccgccacg gcatccttgg acagatagcc ctgctccgcc tgcacgtcct
     5701 gaagcatggg aatcagcgaa tcgatgcccg cccggcggta tttcttcaga atgctgtcca
     5761 tggccctgct cattttggag ctcccctggc cggcgccagc tggcgagtga agaacgaaac
     5821 cttctccagc gccatggtca tatccatatg ctccacatac gttttgggcg gcacgcgcac
     5881 cggggtcggc gcaaaattca gaaggccctt gatgccggcg gcaaaaaggc ggtcggcgac
     5941 gcgctgagca tcgggccccg gcacggcaat gaccgccacc tgtatgccct gctccgcgat
     6001 caccttttca accatttcaa tgtcgaagca gcggcagccg tggatcacgc gtccggtctt
     6061 gtgcgggtcg cggtcgaaag cggccacgac ctgcagattc gagagcgaac cctggaagta
     6121 ggtcagaagg gcgcggccga gattgccgat gccgcaaagg gccaccttct cgccgccggg
     6181 acgatcaaga taagagccga tggccgccag cagatcttcg acatcatatc cgcgcgtgga
     6241 ttttccgcgg tagcccatct ccatgagatc gcgccgcacc tgagccgact gcccatggca
     6301 gcgttccgcc agcgtgctgg atgaaacgtg gtccacattc tccttgagga attccctgag
     6361 gaagtgccgg tactgactca agcgttcaat tgttttctcg gaaatcataa ctccaccgtt
     6421 tgatcataaa cataataaaa catatccaac aaaggatact gtgaaaacat tcacaatcat
     6481 catacgcgct ttcgatactg ggtcaaccgc ttttttgtga tttttttcac aggaatggcc
     6541 gccatgcagc ctgtttaaaa aacagacacc ttcccctgtt ctccggaagg cgggtcaggc
     6601 gaaatccggc cgattttcta atatattccc gctttttatc ttatattagt ttgcaaaatc
     6661 gtttgacgcg gcagagggtt aaaaggtatc aacaacgcgg tagtttttaa ttttcggagg
     6721 atatcatggc caagatactg attgttgatg acgatcccga tgtggtggaa gccagttccc
     6781 tggttctcgc cggcgcaggc tacgaagtag gcacagccag cagccgcgcg gccggcatgg
     6841 aacaaattca gagtttcaag cccgatgcgc tgattctcga tgtcatgatg gacgaacccg
     6901 atgacggcat cgccatggcc caggaactgc gccgcaccgg attcacccgg cccatcctga
     6961 tgctcaccag cctcggcaag gtgcttggca tggatttcgg caaggacgaa tccgtcgtcc
     7021 ccgtggacga ttttgtcgag aagcccatca atcccggcga cctcgtcgcc cgggtgaaaa
     7081 aactgctggc cgcaaagaaa ggaaagtaat ccccatgctg acaccagaaa gagaccagct
     7141 tgctgagaat attacgaagt ggattgatca gtacggcggc aaccgcagcg cgctgctgcc
     7201 gaccctgcag gaaatccaga agcgtcacgg gcgcgtcacc gagtacgcca tgcaggtggt
     7261 ggctgacaaa ttgagcattc atccggttga agtctacggc gtgatcacct tctacgcgtt
     7321 tctcgatacc aagtacaagg gccgctttat catccggctc tgccgcacaa tctcgtgtga
     7381 catggccggc aagtcccgcg tggcccgcca gctcgaaaac gatctgggca taaaattcgg
     7441 cgagtccacg gccgacgggc gcttcacgct cgaatgggcg aactgcctcg ggctctgcga
     7501 ccacgggccg gccctactgg tcaactcgca ggtgttcacg catgtcacgc ccgagaaggt
     7561 ccacgacatc attgaaagct gccgcaaagc cttcggggcc cacacgctcc agaaggagga
     7621 ggtacacata gcatgagcga aacctacaca gcccctttga cattcagcaa ggtgaaggcc
     7681 ggtaaaggcc tcgaagccgc cctggaactc gatcgcgcag gcgtcatcaa tgaaatcacc
     7741 gcttccggcc tcaagggccg cggcggagcc ggatttccga ccggcgtgaa gtggaacctc
     7801 gcggccgccg cgaaggaccc ggtgaaatat gtggtctgca acgccgacga aggcgaaccc
     7861 ggcaccttca aggaccgcgt gatcctgacc gatcacgccg acctcgtgtt cgagggcatg
     7921 accatctgcg cctatgcgat cggcgcaaag aaaggcattc tctatctgcg aggggaatac
     7981 acctatctgc aggaccacat cgaaggcatc ctccgcaagc ggcgcgaagc caaactgctc
     8041 ggcaagaacg ccggcgggaa aaaggggttc gagttcaaca tcgaagtccg catgggttcc
     8101 ggcgcctata tctgcggcga ggaaaccgcg ctgatcgagt cgctggaagg ccagcgtggc
     8161 gaaccgcgca accgcccgcc cttccccgtc aacaccgggt tcctgggcca tccaacggtc
     8221 cccaacaatg tcgagacctt cgcatgggcc gcaacgatca tgagcaaggg ctccgagtgg
     8281 ttcaagaaga tcggcaccga caagtcggcc gggctgaaga tcttcagcgt atcgggtgac
     8341 tgcaagcagc cgggtgtcta tgaattcccg ctgggcatca cgatcaagga actgctgaag
     8401 gccgtcggcg gactggacgc caaggccgtg caggtcggcg gcgcttccgg cagctgcgtg
     8461 cccgcacgcg agttcaaccg caagatcgcg ttcgaggatg tcgccaccgg cggatccatt
     8521 atcgtcttcg gcccgcagcg cgacatgctg aaggtggctg aaaacttcat ggagttcttc
     8581 catgacgagt catgcggaca gtgcaccccc tgtcgcgagg gcaacggaaa gatacttgag
     8641 ggcatccaca tgctccagca gggcaagtgc tccatgcact acctgcgtga actgtgcgcc
     8701 ttgggcgaaa ccatgcagct cgcctccaag tgcgggctgg gccagaccag cccgaacgca
     8761 tttctttcca tcgtgaagaa tttcccggat gaaatcatgg gccgtgccgg cgcagctcag
     8821 gaatgaccca agcgaggaac cgatcatgac agacaaaaac ataaagctca aagaagaatc
     8881 acgcgcaccc gtcagcagtt ccgtgcagaa gttcgccgcg ccccaaaacg agaaggccat
     8941 cggcgcgacc atcacggtca caattgacga caagcagatc aaggtgccga tgggcaccac
     9001 gattctggag gccgcgaaga agctgaacat tcacattccg acgctgtgct atcacgagga
     9061 tctctgtgtg gccggcgtct gccgcatctg tgccgtcgag gtggaggggc agcggacact
     9121 tcaggcatcc tgcgcttatc ccatcaccgc gcccatcacc gtccgcacgc acacccgcaa
     9181 ggtgcgccag gcccggcggc acatcctcga cctgctgctc tcgaagcact atggggaatg
     9241 ctacgcctgc gcccgcaaca acaactgcga gctgcaggcg ctggccaagg agtacggcgt
     9301 agacttcttc cgcttcgggc atcccgataa accccgcttt gaggttgacc gctccagcta
     9361 ctccgtcgtg cgcgacatgg acaagtgcat cctctgccgc cgctgcgtgc gcacctgcat
     9421 tgatctgcag gaggtcggca ccatcgaggt gatcgagcgg ggcgccaaga gccacatcgg
     9481 caccttcctg gagaagcccc tgggcagcgt ggtgtgcatc aactgcggg
//
LOCUS       DAOQWR010000101         9413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7787_length_9413_cov_5.561979, whole genome shotgun sequence.
ACCESSION   DAOQWR010000101 DAOQWR010000000
VERSION     DAOQWR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7787_length_9413_cov_5.561979"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PKK36_08480"
     CDS             complement(<1..978)
                     /locus_tag="PKK36_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNR94628.1"
                     /translation="MNAKTPAPFVPGGYREVFRVAWPLIISMGSFTLMTFADRIFLAR
                     FSTTAIQAAVPSGIMVFCFCCGFMALASYSSVFVSQYFGAKDYPLCSVATAQGVLLAL
                     ATWPILILMIPLGRWLLAVSGHAPEVLELELVYFTILMLGSGTTALGAAIAGFFTGRG
                     DTRTTMITNVIGNGLNIVLDYAMIFGKWGFPQMGIAGAALATVIAGFVSPLILLALYF
                     NKRFVAVFQTRSTFRYNHRMFWRIVRFGFPSGVHLALDIASFTLFVVLTGRLDPAGLA
                     ASNIALSINTLAFMPLIGLGIAASILVGQYIGRREPEHAERAAWNAWQIG"
     gene            complement(968..2308)
                     /locus_tag="PKK36_08485"
     CDS             complement(968..2308)
                     /locus_tag="PKK36_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNR94629.1"
                     /translation="MSFHAAQKHFHLAGAAGVGMSAMAELLLSLGAEISGSDRFHDQR
                     QDLPVLRTLAARGMHLVPQDGSGIRCGTAALICSTAIEGDNPEIARARSLNIPIITRG
                     DMLGRLSAGHRCIAVAGTSGKTTVTGMTGWLLAELGADPTVVNGGAVIGWDEGEHVGS
                     IRMGKSGLWVAEADESDRSCLFLHPEWNIITNISRDHFPLPETIELFRQFASQTSERV
                     VCFPETAKLLSNSLAVENTVFRNGKSWSFILNKLEITSPLPGRHNAENALLACALCIA
                     TGYDAEAVRKALASFKGIRRRLELVGEARGIRVFDDYAHNPAKIAAAWSTIAEDSSRV
                     LGVWRPHGFGPLRQMKEALAEMFRAELRGNDQLFLLPVYYAGGSAEKDVSSDELAAAL
                     KAQGVNASAVPDYQELTRLILQEADAGGSVLVMGARDPMLPVFARDVLKRLNER"
     gene            complement(2438..3337)
                     /locus_tag="PKK36_08490"
     CDS             complement(2438..3337)
                     /locus_tag="PKK36_08490"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNR94630.1"
                     /translation="MIAACLGLLGPLMAGAQEVPAATNELLVEEEPLVIEEVITRPPD
                     PLPTHRGSDMPDAFKGADPSRFGVSGRVHKMPAKKSKKREKGCWRGKLDLGLNTASGN
                     SDILRYNGSLTGSKETEENYYFLEAAGRYGESDNEKDTEHATGEVKYHHRLSERMYSA
                     VDGNVMHDGIADVSYRARGSLSLGRKLIWTDRTVLTLEAGPGYVEERKGGEETGFVAG
                     RIAQYMEILLTDSLQVWQSVEYFADITDSAVYFINAEAGLETVLIANLSLRFTVEDRY
                     DSNPAEDKKSNDLVTTTAFVWNF"
     gene            complement(3402..4340)
                     /locus_tag="PKK36_08495"
     CDS             complement(3402..4340)
                     /locus_tag="PKK36_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNR94631.1"
                     /translation="MPELKNTFAWSFSAAEAFDACPRKRYWGVYARWGGWAPGASEIQ
                     RKAWQLSRMDTLPALRGKAVEKAIMWALREMQAGRTVTPDQAYEQIAKPWLNTCWTQS
                     KQGLWRTDCKANCCLREHYCGQWNADQEKEAVTTEVANIRRCIGNFTTRVWPRLKDIS
                     PADEIAIGGPGEEFFMLHDVKINAIPDYAVRTADAVRIHDWKAGKPSEKHRTQLNIYG
                     LWAKMKHGAVPENTLLFVEYLSDGKLAAWAFSEEELRETMEWIETSVAAMTGYLENED
                     RAGNIPLPREQWDLATEPELCRNCNFYELCKPELGF"
     gene            complement(4401..4706)
                     /locus_tag="PKK36_08500"
     CDS             complement(4401..4706)
                     /locus_tag="PKK36_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HNR94632.1"
                     /translation="MKIILTVIASVMISLSALAQEEAAAPAETASTEAVAVESSLIKS
                     VAYNAETRVLTVVMVKNDETYEYSDVPELIYKELMEAESKGSYFIKNIKSTYKTVKK"
     gene            4918..5232
                     /gene="rplU"
                     /locus_tag="PKK36_08505"
     CDS             4918..5232
                     /gene="rplU"
                     /locus_tag="PKK36_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR94633.1"
                     /translation="MEAYAVIETGGKQHRVQKGDVIKIERIEGEAGSKVTLSPVLAIS
                     DGASLKVGTPVIEKAKVTAQIFEQMRDKKVVAFKKKRRKGYKRKVGHRQEITQVKIVS
                     LS"
     gene            5251..5508
                     /gene="rpmA"
                     /locus_tag="PKK36_08510"
     CDS             5251..5508
                     /gene="rpmA"
                     /locus_tag="PKK36_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR94634.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGVKKFGGQPVRAGNILVRQVGTR
                     ILPGVNVGMGRDYTLFALKDGVVRFDRSGKRVNIEKGEPVS"
     gene            5600..6637
                     /gene="obgE"
                     /locus_tag="PKK36_08515"
     CDS             5600..6637
                     /gene="obgE"
                     /locus_tag="PKK36_08515"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNR94635.1"
                     /translation="MKAVTFKDRVRLYVTAGNGGDGSASFRREKFIPKGGPDGGDGGR
                     GGDITLCASKDVDSLVALYFTPHQRAGHGGSGRGGQCSGKNGKSLDIPVPCGTVVFNA
                     DSGEKVGEVISDGETLRVARGGRGGLGNVHFKSSTHQAPREFTKGEKGEEFTLNLELK
                     MVADVGLIGYPNAGKSTLLSKISRARPKIAAYPFTTLNPVIGTVELDDTRRIRVADIP
                     GLIEGAHKGVGLGHDFLRHIERTRYLLFVIDMAGVDGRKPVEDYFSLRSEMRLYRPEL
                     DERPYFIVANKMDMPEAAENLAEFRLTAGENPIEVSAELGEGIDKVVEILRGCAVSEP
                     PTSAGEPDAEA"
     gene            6624..7538
                     /locus_tag="PKK36_08520"
     CDS             6624..7538
                     /locus_tag="PKK36_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR94636.1"
                     /translation="MPKHKPSHYAEYAVLRLLGWILCILPYYAALALGCLAAKISFLL
                     MGRKMARAKARVKQVLGPETGEDEASRIVWVAWRNLVFNGIEMLRAPRVNLRWVNKYT
                     DYSQFERVLAKLKSGQNVILALPHMGSWETAGLSASLLGVPLLFITRQQKNVLIGNFI
                     NRIRSRTGQECVDRDLPLLRIILKKIKEGKVLAILPDIRARTDAMQVDFLGGRPMIAA
                     GMALFARQANVPIYSGCVVRTGWGHHKWIVYDEIAPDPSLEKTADWHRITQAVMSNFD
                     HLIREHPGQYFWFNKKWILEPPAENETD"
     gene            7576..7800
                     /locus_tag="PKK36_08525"
     CDS             7576..7800
                     /locus_tag="PKK36_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR94637.1"
                     /translation="MKTKVRKIGNSYGIVLPKEALHSMKVKEGDTLYLTEAPECSLRV
                     TPGDRQFKDMMAIAERGMTRYRNALRELAK"
     gene            7797..8186
                     /locus_tag="PKK36_08530"
     CDS             7797..8186
                     /locus_tag="PKK36_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNR94638.1"
                     /translation="MKSPLWVPKAVVIAAHEELLDRFGGLSGIRDEGLLDSALARPEH
                     LWAYGQPDLFDLAAAYAAGIVKNHPFIDGNKRSGFIAAYTFLESNGETFTAPEEEVVL
                     RTLALAAGRIRERQYVAWLRKSCARKS"
     gene            complement(8192..8899)
                     /locus_tag="PKK36_08535"
     CDS             complement(8192..8899)
                     /locus_tag="PKK36_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94639.1"
                     /translation="MCDLCNQLKERETSYSKYGSPDDDRPLPEAAAKLTVLKTLNTKD
                     LDKTHVRRCPRCGTLYRYLASHEYFINGSEDEEMLTRMTTEEAAAWLREEALTLESLR
                     TDIELYRDRGGSLGDYIDHGQPAPAEIAEAMDEMRMCSKKAGELTALLDEQVTALRRD
                     CPEILSLWSQAHDRVCLHYMKTLPKTGDDAKTARHIAGEARTSWRRLPADGDSFISVA
                     AVWLPGYEERIRTEIQK"
BASE COUNT         1929 a         2701 c         2819 g         1964 t
ORIGIN      
        1 gccgatctgc catgcgttcc aagcggccct ttccgcgtgc tccggctcgc gccggccgat
       61 gtactgaccg accagaatgg aggcggctat gcccaggccg atcagcggca tgaaggccag
      121 cgtgttgatg ctgagcgcta tgttgctggc tgccagcccg gcgggatcga gacggccggt
      181 cagcaccaca aacagggtga acgacgcgat atccagcgcg agatgcacgc ccgacggaaa
      241 cccgaaccgg acaatgcgcc agaacatccg gtgattgtag cggaacgtgc tgcgcgtctg
      301 gaacacggcg acaaagcgct tgttgaaata cagggccagc aggatcagcg ggctcacaaa
      361 gcccgcgatg acggttgcca gggcagcgcc cgcgatgccc atttggggaa atccccattt
      421 cccgaaaatc atcgcatagt cgagcacaat gttcagcccg ttgccgatca cattcgtgat
      481 cattgtggtg cgggtgtctc cgcggccggt gaaaaagccg gcgatggccg ctcccagcgc
      541 ggtggtgcct gagcccagca tcagaatggt gaaatacacc agttccagtt caagcacttc
      601 gggcgcatgg ccgctgacgg ccagcagcca ccgccccagc ggaatcatga ggatcagaat
      661 cggccatgtg gcgagcgcca gcagcacgcc ctgcgcggtg gccacgctgc agagcggata
      721 atccttcgcc ccgaagtatt gcgagacgaa gacggaggaa taagaggcca gcgccatgaa
      781 tccgcagcag aagcagaaga ccatgatgcc ggaaggcacg gccgcctgaa tggcggtggt
      841 gctgaagcgc gcaaggaaaa tgcggtccgc gaaggtcatc agggtgaaag agcccatgct
      901 gatgatcagg ggccaggcca cgcggaaaac ctcgcgatag ccgccgggca cgaagggggc
      961 tggagtttta gcgttcattc agcctcttta gaacatcgcg cgcgaaaacc ggaagcatcg
     1021 ggtcgcgcgc ccccataacc agaacggaac cgccggcgtc tgcttcttga agaatcagtc
     1081 tggtcaattc ctgatagtcc ggaaccgccg aagcgttcac cccctgcgcc ttcagcgcgg
     1141 cggcgagttc atccgaggaa acatcctttt ccgcgctgcc gcccgcatag tagaccggca
     1201 gaagaaaaag ctgatcattc ccgcgaagct cggcgcggaa catttccgcg agcgcctcct
     1261 tcatctgccg cagcgggccg aatccgtgcg gccgccacac gcccagcacc cgcgaagaat
     1321 cttccgcaat ggttgaccag gccgcggcga tcttcgccgg attgtgggcg tagtcgtcaa
     1381 aaactctgat gccgcgggct tcgccgacaa gctccagccg gcggcgtatg cccttgaagc
     1441 tcgccagcgc cttccgaacg gcttccgcat cgtaacccgt cgcgatgcag agcgcgcagg
     1501 ccagcagggc gttttcggca ttgtgtcgtc cgggcagggg ggatgtgatt tccaatttgt
     1561 tcaaaatgaa agaccagctt ttcccgtttc tgaaaaccgt gttctcaacg gcgagactgt
     1621 tggaaagaag tttcgcggtt tccggaaagc acaccacccg ttctgatgtc tgcgatgcga
     1681 actgccggaa gagttctatg gtttcgggga gggggaaatg gtcgcgggag atattggtga
     1741 tgatgttcca ttccggatgc aggaaaaggc aggagcggtc gctctcgtcg gcctcggcca
     1801 cccacaatcc ggatttgccc atgcgtatgc ttccgacgtg ttcgccttcg tcccagccga
     1861 tcacagcgcc gccgttgacg accgttggat cggccccgag ttccgcgagg agccagccgg
     1921 tcatgccggt gacggtggtt ttgccggaag tcccggcgac cgcgatgcag cgatgccccg
     1981 cggaaagccg gcccagcatg tccccgcgcg taatgatcgg aatgttcagg ctcctggccc
     2041 tggcgatttc gggattgtcg ccttcgatcg cggtgctgca gatcagcgcg gcggtgccgc
     2101 agcggatccc ggacccgtcc tgtggaacaa gatgcatgcc ccgcgcggcc agtgtgcgga
     2161 gcacgggcag atcctgccgc tgatcatgaa agcggtcgga ccccgaaatc tccgccccaa
     2221 gagacagaag cagctcggcc atggcgctca tgccgacgcc ggccgcgccc gccagatgga
     2281 agtgcttctg tgctgcatga aaagacatgc ggcctataat agaggggaaa gtccgcttga
     2341 acagaaaatt tccaatgatt ggaacttttg tttgaaaaac ttccaatgat tggaactttt
     2401 tgcgcggaaa cttccaatga ttggaacttt tgagcggcta gaaattccag acaaaggccg
     2461 tggtggtcac cagatcattg cttttcttgt cttcggccgg gttgctgtcg tagcggtctt
     2521 cgacggtgaa gcgcaggctc agattggcaa tcagcactgt ttcaagcccg gcctctgcat
     2581 tgatgaaata aacggccgaa tccgtgatgt ccgcgaaata ttcgaccgac tgccagacct
     2641 gcaggctgtc ggtgagcagg atttccatat actgcgcgat gcgcccggcg acaaagccgg
     2701 tttcctcgcc gcccttgcgc tcctccacat atccgggacc cgcttccagc gtcagcacgg
     2761 tccggtccgt ccagatgagt ttccggccca gagacaggct gccgcgcgcg cgataggaaa
     2821 catctgcgat tccgtcgtgc ataacgttgc catccacggc ggaatacatc cgctccgaca
     2881 gccggtggtg atacttgact tcgccggtcg cgtgttcagt gtctttttcg ttgtcgcttt
     2941 ccccgtagcg gcccgcggct tccagaaaat agtagttttc ttccgtctcc tttgaaccgg
     3001 tgagggatcc gttatagcga aggatgtcgc tgttgcccga tgcggtattg agtcccagat
     3061 ccagcttccc gcgccagcag cccttctcgc gctttttgga tttcttcgcg ggcattttat
     3121 gcacacgccc gcttacgccg aatcgtgaag gatccgcgcc cttgaaggcg tcgggcatgt
     3181 cggagcccct gtgagtgggc agggggtccg gcggccgtgt gatgacttcc tcaatgacga
     3241 ggggctcttc ctccaccaga agttcattgg ttgcggcggg aacttcctgc gccccggcca
     3301 tcagcgggcc cagcagtcca agacatgcag cgatcatgat cagatttttc atgggcacaa
     3361 aggctaaacc ggaattctgt ccgccgtcaa tccgcgaacg ttcagaaccc cagctcgggt
     3421 ttgcagagct cgtagaagtt gcagttgcgg cagagttccg gttcggtggc gagatcccat
     3481 tgttcgcgcg gcagggggat gttgcccgcc cgatcctcat tctcgagata tccggtcatg
     3541 gcggccacgg aagtttcgat ccattccatc gtttcccgca gttcctcctc gctgaaggcc
     3601 caggcggcca gtttgccgtc cgagagatat tccacaaaca gcagggtgtt ttccgggacg
     3661 gccccatgct tcatctttgc ccacagcccg tatatattga gctgtgtgcg gtgtttttcg
     3721 gacggcttgc ccgctttcca gtcgtggatt ctcaccgcgt ccgccgtgcg aacggcgtag
     3781 tcggggatgg cgttgatctt cacgtcgtga agcatgaaaa actcctcgcc gggaccgccg
     3841 attgcgattt catctgcggg agaaatatct ttcaggcgcg gccagacgcg ggtcgtgaaa
     3901 ttcccgatgc agcggcggat gtttgccacc tcggtcgtga ccgcctcctt ttcctgatcg
     3961 gcgttccatt gcccgcagta atgctctcgc aggcagcagt tggccttgca gtcggtacgc
     4021 cagagaccct gtttggactg ggtccagcag gtgttgagcc agggctttgc aatctgctcg
     4081 taggcctggt cgggtgtgac cgtccggccc gcctgcattt cgcgcagggc ccacatgatc
     4141 gctttttcaa cggcttttcc ccgcagggcg ggaagcgtgt ccatgcggct gagctgccag
     4201 gccttgcgct gaatttccga tgcgccgggc gcccagccgc cccagcgggc atatacgccc
     4261 cagtagcgct tgcgggggca ggcgtcaaaa gcttccgcgg ccgagaacga ccacgcaaag
     4321 gtgttcttca gttccggcat ctcaacttcg tgtcacttcg tccggcttcc gcaggcgggc
     4381 tgggaagagc ccgcgcggaa ctacttcttg acggttttat aggtgctctt gatgttcttg
     4441 atgaagtagg aacccttgga ttcggcttcc atcagctctt tgtagatcag ttcgggaaca
     4501 tcggaatatt cataggtttc atcgttcttc accatgacca cggtcagcac gcgggtctcg
     4561 gcattgtagg ccacggactt gatcaggctg gactccacgg ccacggcctc ggtcgaggct
     4621 gtttcggcgg gcgcggcggc ttcttcctgc gccagggcgg aaaggcttat cattacggac
     4681 gcaattacgg tcagaattat cttcatgctt ggttctccta atggttgaaa aacacaggct
     4741 gactatcgca aaccggccgg aaaatagcaa aacctatcgg aaaacggcgg gggcagaaaa
     4801 agtgcctcga aagcgggtgg ggtattgaca atccccggca tctcccgtac attttgcggc
     4861 cttgtttgag aataccagga tttcccggaa aatcagcggg aagaaatgag gcttattatg
     4921 gaagcgtatg ccgtaattga gaccggtgga aagcagcacc gtgtccagaa aggcgatgta
     4981 atcaagattg agcggatcga aggcgaagcc ggatccaagg tgaccctgtc gccggtgctg
     5041 gcgatttccg acggcgccag cctgaaggtg ggcaccccgg tgatcgaaaa agccaaggtg
     5101 actgcgcaga ttttcgagca gatgcgcgac aagaaggtcg tggctttcaa gaaaaagcgg
     5161 cgcaagggct acaagcgcaa agtcggccac cgccaggaga tcacgcaggt caaaattgtg
     5221 agtttgagtt aagcgggaag aggtacagcc atggcacata aaaaaggtca gggatcaagt
     5281 cgtaacggcc gtgacagcaa cagtcagcgg cgcggtgtaa agaagttcgg cggccagccg
     5341 gtgcgcgcgg gcaacattct tgtccgtcag gtcggcaccc gcattctgcc gggcgtcaat
     5401 gtggggatgg gccgcgacta cacgctgttc gcgctcaagg acggtgtggt ccggttcgac
     5461 cgcagcggca agcgcgtgaa catcgagaag ggcgaacccg tttcctgaat cagaacggat
     5521 atttgcgcgg aggcggaccg ttgacggtcc gcctctttta ttgccttgtt ccggcggata
     5581 gactacaaga cgatagttta tgaaagcagt caccttcaag gatcgcgtgc gcctctatgt
     5641 caccgccggc aacggcggcg acggttcggc ctcgttccgc cgcgagaagt tcattcccaa
     5701 gggcgggccc gacggcggcg acggcggccg gggaggcgat atcacgcttt gcgcaagcaa
     5761 ggacgtggat tcgctggttg ccctctattt caccccgcac cagcgcgccg gtcacggagg
     5821 cagcggccgc ggcggtcagt gctcgggaaa gaacggcaaa tcgctggata tcccggttcc
     5881 ctgcggcacc gtggttttca acgccgacag tggggagaag gtcggcgaag tcatctccga
     5941 cggcgagacg ctgcgcgtgg cgcgcggcgg ccgcggcggg ctgggcaacg tgcattttaa
     6001 atcctccacg catcaggcgc cgcgcgagtt tacaaaaggt gaaaagggcg aggagttcac
     6061 gcttaacctc gagcttaaga tggtcgccga cgtcgggctg atcggctatc ccaatgcggg
     6121 aaagtccacg ctgctgagca agatcagccg tgcccgcccg aagatcgcgg cctatccctt
     6181 caccacgctg aatcccgtga ttggaaccgt tgaactggac gacacccgcc gtatccgcgt
     6241 ggccgacatt cccgggctga tcgagggcgc gcacaagggc gttggcctcg gccacgattt
     6301 cctccggcat atcgagcgga cgcgttatct cctcttcgtc attgacatgg ccggggtgga
     6361 cggacgcaag cccgtcgagg actatttcag cctgcgaagc gaaatgcggc tttacaggcc
     6421 cgagctggat gagcggccct attttattgt ggccaacaag atggacatgc cggaagccgc
     6481 tgaaaacctt gctgaattca ggctcaccgc cggcgaaaat ccgattgaag tctcggctga
     6541 gctgggcgaa ggcatagaca aggttgtcga gatactcagg ggctgcgccg tttccgaacc
     6601 tccgacatcc gcgggagagc cggatgccga agcataagcc atcccattat gcggaatacg
     6661 cggttcttcg actgctcgga tggattctct gcattctccc ttattatgcc gctcttgcgc
     6721 tcggctgcct ggccgcaaaa atatcctttc ttttgatggg ccgtaaaatg gcccgcgcga
     6781 aggcgcgtgt gaaacaggtt ctgggccctg aaaccggtga agacgaggcc tcgcgtattg
     6841 tctgggtcgc atggcgcaac ctggttttca acgggatcga aatgcttcgc gcgccgcgtg
     6901 tgaatttgcg ctgggtcaac aaatacacgg actactccca gttcgagcgg gtcctggcaa
     6961 agctgaaatc cggacagaat gttattctgg ccctgccaca catgggaagc tgggaaaccg
     7021 ccggactttc cgccagcctg ctgggggttc cgcttctgtt cattacccgc cagcagaaga
     7081 atgtgctgat cggcaatttc atcaaccgca tccgcagccg caccggtcag gaatgcgtgg
     7141 accgcgatct gccgctgctg cgcatcatcc tcaagaaaat caaggagggg aaagttcttg
     7201 cgattctgcc cgacattcgc gcgcgcacgg acgcgatgca ggtggatttc ctcggcggcc
     7261 gcccgatgat tgccgccggc atggctcttt ttgcgcggca ggccaatgtg ccgatctact
     7321 ccggctgcgt cgtgcgcacg gggtgggggc atcacaagtg gatcgtttat gatgaaatcg
     7381 ctcccgatcc ttcgcttgag aaaacggccg actggcaccg catcacgcag gccgtcatga
     7441 gcaatttcga tcacctgatc cgagagcatc ccggccagta cttctggttc aacaagaaat
     7501 ggatccttga accgcccgcg gagaacgaga cggattgaca aaacggcagg ttataactaa
     7561 agttataaca tgacgatgaa aaccaaagtt cgcaagatcg gcaattcgta cgggattgtg
     7621 ctcccgaagg aagccctgca ctccatgaag gtcaaggaag gcgacaccct ctatctgacc
     7681 gaggcccccg aatgttccct gcgtgtgacg ccgggggacc ggcagttcaa agatatgatg
     7741 gcgatcgcgg agcgaggcat gacacgttac cggaatgctc tgcgtgaact ggccaaatga
     7801 aatcgccgct gtgggttccc aaagcagttg tcattgctgc gcatgaagag cttctcgatc
     7861 gtttcggagg gctttcgggt atacgcgacg agggtttgct ggattctgct cttgcccggc
     7921 ccgaacatct ttgggcgtat ggtcaacctg acctgtttga tcttgccgcc gcctatgccg
     7981 ctggaatcgt caaaaatcat ccatttatag atggtaacaa gcggtctggt tttatcgcgg
     8041 cctacacgtt tcttgagtcg aatggtgaaa ctttcaccgc ccccgaagag gaggtggtcc
     8101 tgcggactct tgcccttgca gcaggaagga ttcgtgaacg ccagtacgtc gcatggctcc
     8161 ggaaatcctg cgcccggaaa tcgtgatagg cttatttctg tatttcggtc ctgatgcgtt
     8221 cttcatatcc cggaagccag acggccgcta cggatataaa tgaatcgccg tcggcgggaa
     8281 ggcggcgcca gctggtcctc gcttcgcctg cgatgtggcg ggcggttttg gcgtcgtcgc
     8341 ctgttttcgg aagcgttttc atgtagtgca ggcagacgcg atcatgcgcc tgtgaccaaa
     8401 gcgaaagaat ctccgggcag tcgcgccgca aggcggtgac ctgttcatcc agaagcgccg
     8461 tcagttcgcc ggccttcttg ctgcacatcc gcatttcatc catcgcttcg gcgatttcag
     8521 ccggagccgg ctggccgtgg tcaatataat ctccaaggct tccgccgcgg tcccggtaca
     8581 gttcaatgtc tgttctaagg gattcaagtg tgagcgcttc ctcccggagc cacgcggccg
     8641 cctcctctgt ggtcattcgg gtcagcattt cctcgtcttc acttccgttg atgaagtatt
     8701 cgtgggacgc gagataacgg taaagcgttc cgcaccgggg gcagcgccgg acgtgtgttt
     8761 tgtccagatc ttttgtgttc agcgtcttca ggacggtcag cttcgccgca gcctccggca
     8821 gcggccggtc atcatccggc gaaccgtact ttgaatatga agtttcgcgt tccttcagct
     8881 gattgcacaa atcgcacata aagccgcccc gtcagaaagc actccccgca tgaaaatcat
     8941 aatccatgga tggctcagcc tattctcctg cacccggtaa ccgcctgttc ccataaacgc
     9001 ggactttacc ccgacgggaa atccaaacca agcgccgagt ggcaaaaacg ccattgataa
     9061 cccggggggg cgatcccttg ccagcgcata ttttgtgcat tgtgcgcctt tttgcggcca
     9121 tcttctcatg agcttcatcg tgaaatcttt tctgaatctc tgcggcttcg tggctttgtg
     9181 cgatatatct aatctggaac tcaggaagac aggaacggat ttatcgcgca gtgacgcaga
     9241 gaataatttg acaggatcaa caggattccg gaaaccacgg attgcacgga ttgcacggat
     9301 gaagagggtt tcaagttgca ggtaccaggt tgaaagtgga ggatgtccgg gaaaatgaat
     9361 cttgattcag gacgcgcgcg gctggggcat gattcctgca acctgtaact tgc
//
LOCUS       DAOQWR010000102         9366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7839_length_9366_cov_5.449683, whole genome shotgun sequence.
ACCESSION   DAOQWR010000102 DAOQWR010000000
VERSION     DAOQWR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7839_length_9366_cov_5.449683"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKK36_08540"
     CDS             complement(<1..338)
                     /locus_tag="PKK36_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94640.1"
                     /translation="MPSNDMPRGLQNFFLICASTAFLLPSGCATSRARWPVKLDSRPA
                     VSIETFEDHSGFRGKWKPGEDMARLAADALRKTDRVEVHVPEVRAGVEKILDQGKNLL
                     LNDGASDSCA"
     gene            complement(325..678)
                     /locus_tag="PKK36_08545"
     CDS             complement(325..678)
                     /locus_tag="PKK36_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94641.1"
                     /translation="MIVVCDDDKVLTHLLTRLLKDAGYEVCVAHDGQSAYHLAREPKC
                     RGMLLDMRMPNINGAELLMLLAAEHIAVPVIVMAGFPDYNEEELKQFPNVKKFLQKPL
                     NPDDVLEAVEQYALK"
     gene            complement(940..2091)
                     /locus_tag="PKK36_08550"
     CDS             complement(940..2091)
                     /locus_tag="PKK36_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNR94642.1"
                     /translation="MIKTVCASSVAQGAAAFAQFGPVEIIPDSEITPQAVSSATALVV
                     RSKTKVGPDLLKGSRVRFVGTATAGFDHFDRAWLDHAGIVSASAPGCNANSVAEYVMA
                     ALLCIAAEKRLDLDEMTLGVVGCGNIGSRVARRAEAIGIRVLKNDPPLAAETGNPDFI
                     PLEHLLKESDIVTLHVPLTTGGPWPTAGLAACPFFSQIKPGAVFINTSRGEVVDESAL
                     TLSLQNDAVSAAVLDVWRNEPVFSRTLLDLADIGSPHIAGYSAQGLLNGTMAVYQELC
                     HFLEEPPAWKPEPAAAPLPPVEMDTRGLSHEEVLHQMSRSVYDIRADDTLLRRAAAAD
                     DETRGRNFTLLRSGYPVRHEFADARIRLLHADRILTEKAKGLGFCNNPC"
     gene            complement(2170..3234)
                     /gene="corA"
                     /locus_tag="PKK36_08555"
     CDS             complement(2170..3234)
                     /gene="corA"
                     /locus_tag="PKK36_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNR94643.1"
                     /translation="MSRHMKSRRRALQSPPEALVYIGSNPNANEMIDVFSYNKGHFEE
                     TQVHADQFPSGPLPRGHVTWVNVDGVSDVNLIRRIGAQFGFHELVLEDIVHTGQRPKM
                     EDHGSYIYCVLRMLYNKDHDDHIESEQVSLLLTQDAVFTFQEAQGDVFDPVRKRLREK
                     NSHLAEHGADYLAYSLIDMIIDNYYLILEDIGDRIERLEEEVSGRPDPETLKNIHGVR
                     SDLIHIRKAIWPLRELTSDLQRCDSPLIRRETSRFIGNLRDHMLQIMDAVETFRDTTG
                     GLLDIYISQISFHMNEIMKTLTITATIFIPLTFVAGVYGMNFRHMPELDWRWGYAMSW
                     AIMSGIALFMLLLFRRKKWI"
     gene            3453..4133
                     /locus_tag="PKK36_08560"
     CDS             3453..4133
                     /locus_tag="PKK36_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR94644.1"
                     /translation="MTYIRKLMKRKGFTLIELLVVIAIIAVLAGLLLPAISNARERAN
                     RISCVNNLKQFGTGLYLYEDSYGENNWPAGPGPTNDIRALGKYANAPKMYICKSDKYR
                     SAAARIEDITGANCSYIYLAGYQASDNGNFVVMFDKNGREEPSGILAEDAKLLGATTP
                     PSQANSWGGNHGTEGGNALHVDGHAEWIPVVSDDFNISDLLNEMRFPSNAVYELTVTT
                     CAEVIPAK"
     gene            complement(4310..4783)
                     /locus_tag="PKK36_08565"
     CDS             complement(4310..4783)
                     /locus_tag="PKK36_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94645.1"
                     /translation="MNESQLPQRCHPAHHPPHIRVNHPTILFLTLCVKDKRPLLANTE
                     AQHLLLDGWTKADRWLVGRYVIMPDHIHLFCAPRDLNCRLKAWITYWKNEVTRQWPRK
                     NEKPIWQREFWDTQLRREESYGNKWEYVRQNPVRAGLVESPDDWPFCGEINVLRW"
     gene            5008..6849
                     /gene="glmS"
                     /locus_tag="PKK36_08570"
     CDS             5008..6849
                     /gene="glmS"
                     /locus_tag="PKK36_08570"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNR94646.1"
                     /translation="MCGIIGYVGNEDAQPVILDGLKRLEYRGYDSAGLAVLSGGTLTI
                     RKRVGRLSSLEQTCAENPVQGSPGIGHTRWATHGEPSEVNSHPHTDTAGEIAVVHNGI
                     IENYLELREMLEKEGFVFRSQTDTEVIPNLIALELRRDQKDFMGALSRALQRARGAYA
                     LGILWTREPGRIYAARQGSPLIIGVGEGENFIASDVPAIINRVTRVIYLNDGEMACLE
                     ADGIRLFNLKGEELKPDIQKVTIHAEAASKAGFETFMLKEIHEQPRVLHGLLSKHTSD
                     RQDIAVDLSGLGRDYCRAINRVMIVSCGTAYYAGCYGRLLLELLLDINVEVELGSELR
                     YRRAKLDPGTLIIAVSQSGETADTIAAVKKAKASGCRVLSICNVPGSSLVRESHAVLF
                     TDAGPEIGVASTKAFTAQLMAFALFSLGLARLRGELSDEKLSRCLAELQKVPTHIHYV
                     LSRKDAIRQIADKHHDGPSALYLGRRFNYPIALEGALKNKEISYQHAEGYAAGEMKHG
                     PIALISEYLPVVCICTKTDDELYEKMMSNVREVDARHGRIIAVATDGDEAVRGLAGDI
                     IYVPEIAEEFSPMVNVVALQLLAYYSAQARGTDIDKPRNLAKSVTVE"
     gene            6855..8135
                     /gene="murA"
                     /locus_tag="PKK36_08575"
     CDS             6855..8135
                     /gene="murA"
                     /locus_tag="PKK36_08575"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNR94647.1"
                     /translation="MARFIIRGGNAVGGTMRPVGNKNAALPMIAASLLTDEPVRLSNV
                     PRIEDVRVMLDLLRGTGAEVSQRGGTVTIVSGGGSSGPLNRVLCTKVRSSILLAGPLA
                     ARRGRVVLAPPGGDVIGRRRLDTHFYALRALGIDVRIGSSVTFQRKKLAGAEILLDEA
                     SVTATENAMMAAVLAPGRTVIYNAACEPHVQDLGVMLVKMGARIEGLGTNRIEIRGVR
                     RLHGAAHRVGADYIEIGSFIAAAAATGGALDIPLDGDSRVWLPVVEKMFQTMGISWSV
                     RNGKLHMPARQEKAVQSAAASDIPTISDGIWPAFPSDLMSVAIVLATQSSGTALFFEK
                     LFESRMYFVDHLISMGARIVQCDPHRVVVVGPSRLRPAHMSSPDIRAGMALLIAALCA
                     RGESVIENAQIIDRGYERIEEKLRALGADIRRVE"
     gene            8202..>9366
                     /locus_tag="PKK36_08580"
     CDS             8202..>9366
                     /locus_tag="PKK36_08580"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94648.1"
                     /translation="MKAFHSSDRNSLAEIFSRAGGLFVPGLDGAAAAYFAGALFHDFP
                     CSWLCITDGPRTQETCHRDLQSLFPGHAKRILNYPAWEAYPNLAPEITGERLRAQQAL
                     VENSDSCLVVSCIQALMQPTLSPQELERTVIPLRKGDQADPADLASKLLAAGYRFSHE
                     VFERGQASLRGGILDVWPPDSEYPVRLDFFGDTLDSIRHFNAEEQTSIAKIESVRLSP
                     AMENMAVQFPDPTASLLSYFPEKYGVVFFSQNRIREHGRLLEDSMKAEIGGPGLDAVL
                     QRVEKKAAHAVYLGQGRPPRTPQWESPFHPVESLPRPAGTDYLPDIMEKARREYVEGL
                     LQKTEQGSEVRVFFDTAGTRERFLTMDPRHARFKSCVAPLSAGFSYPAQSLMAI"
BASE COUNT         1867 a         2692 c         2833 g         1974 t
ORIGIN      
        1 tgtgcgcagc tgtccgaggc tccgtcgttc agcagcagat ttttcccctg atcgaggatt
       61 ttttcaacgc cggcccggac ctccggaaca tgcacttcca cgcggtctgt tttgcgcagc
      121 gcatccgccg cgagtcgcgc catatcctcg cccggtttcc atttcccgcg gaatcccgaa
      181 tgatcttcga aggtctctat ggaaacggcc ggacggctgt ccagtttgac cggccatcgc
      241 gcgcgcgaag tggcgcagcc ggacgggaga agaaatgccg ttgaagcgca aatcaggaaa
      301 aagttctgga ggccgcgcgg catgtcattt gagggcatac tgctcaaccg cctcaaggac
      361 atcatcaggg ttcaggggtt tttgcaggaa cttcttcacg ttcggaaact gtttgagctc
      421 ctcttcgtta tagtccggga atcccgccat cacgatcacc gggacggcga tatgttcggc
      481 ggccagcagc atgagaagct cggcgccgtt gatgttgggc attcgcatgt ccagcagcat
      541 gcccctgcac ttcggctccc tggcgaggtg ataggcggac tgtccgtcgt gtgcgacaca
      601 gacctcatat ccggcatcct tgagaaggcg ggtcagcaga tgcgtcagca ccttgtcatc
      661 atcgcatacc acgatcatat tgaatctcca gctttcattc ggattcaggg taaatcaccg
      721 gccggcgttt gtcacgctca gaatggactg gaaacacttc aaggcagcgt ttgcgcgctg
      781 ccttttctga caagaaagga aagtgaggcg cctatttttt cgcggtgttc gcccgctgtt
      841 tcaggcgttt cacgcgacgg tcacgcgctt ttcgttttac tctgactctg agttgctgtc
      901 ccataatgcg cgcgggtgta tcagaaacgg agggcttcgt caacagggat tgttgcagaa
      961 tccgagaccc tttgcctttt cggtcagtat ccggtcggcg tgaaggaggc ggatgcgggc
     1021 gtcggcgaat tcgtgccgaa caggatagcc gctgcggaga agggtgaaat tgcgtccccg
     1081 ggtttcatca tcggccgcgg cggccctgcg caggagggtg tcgtccgccc gaatgtcata
     1141 gacgcttctc gacatctggt gcagcacctc ttcatggctg agtccgcgcg tatccatttc
     1201 aaccggcggc agcggtgcgg cggccggctc cggcttccac gccggcggtt cttccagaaa
     1261 atgacacagc tcctgataca cggccatggt gccgttgaga aggccctgtg ccgagtagcc
     1321 ggcgatatgg gggctgccga tgtccgccag atcaagcagt gtgcggctga agacgggttc
     1381 gttccgccac acatccagca cggcggcgga aaccgcatca ttctgcaggc tgagcgtcag
     1441 cgccgattca tccacgacct ctccgcgcga ggtgttgatg aaaacggcgc ccggtttgat
     1501 ctgggagaaa aaggggcagg cggcaagccc cgcggttggc caggggcctc ctgttgtcag
     1561 cggaacgtga agggtgacaa tgtcggattc tttcagcaga tgctccagag gaatgaaatc
     1621 agggttcccg gtctccgcgg ccaggggcgg gtcgtttttc agcacccgta ttccgatggc
     1681 ttcagccctg cgcgcgaccc ggctgccaat gtttccgcaa ccgacaaccc cgagcgtcat
     1741 ttcatccaga tccagccgct tttccgcggc aatgcagagc agggcggcca tgacatattc
     1801 ggcaacgctg ttcgcgttgc atccgggggc ggatgcgctg acaatgcccg cgtgatccag
     1861 ccacgccctg tcgaagtgat caaaccccgc cgtggcggtg ccgacaaaac ggacccggct
     1921 gcctttcagc aggtccggac cgacctttgt tttggatctc accacaagag cggtggcgct
     1981 ggaaacggcc tggggcgtta tttcgctgtc cgggatgatc tccaccggcc cgaactgggc
     2041 aaatgccgcg gcgccctgcg ccacgctgga tgcacaaaca gttttaatca ttacagtctc
     2101 tcttctgcgc tttcccggta aaagacagcc gtatcacggc atgggtttca agtatccgcc
     2161 tgctcgcagt cagatccact ttttccgccg gaacagcagc agcataaaca gcgcaatgcc
     2221 gctcataatg gcccagctca tggcataacc ccagcgccag tccagctcgg gcatatgccg
     2281 gaaattcatg ccgtagaccc cggccacgaa agtcagggga atgaaaatcg tggccgtgat
     2341 ggtcagggtc ttcatgattt cattcatgtg aaaactgatc tgcgagatgt agatgtccag
     2401 cagtccgccg gtggtgtccc ggaaggtttc caccgcgtcc atgatctgca gcatgtggtc
     2461 ccgaaggttt ccaatgaagc gggatgtttc ccggcggatg agcggcgaat cgcaccgctg
     2521 cagatcggag gtcagttccc gcagcggcca gatggccttg cgaatgtgaa tcaggtcgga
     2581 acggacgccg tgaatattct tgagcgtttc ggggtcgggc cggccggaaa cttcttcctc
     2641 cagccgctct atccggtcgc caatgtcctc cagaatgaga taataattgt caatgatcat
     2701 gtcgatcagc gaataggcca gatagtccgc gccgtgctcg gccaggtgcg agttcttctc
     2761 gcgcaggcgc ttgcggacag gatcaaaaac gtcgccctgc gcctcctgaa aggtgaaaac
     2821 cgcgtcctgg gtcaggagca ggctgacctg ttccgattcg atgtgatcgt cgtggtcctt
     2881 gttgtagagc attcgaagca cacaatagat atagctgccg tgatcctcca ttttgggccg
     2941 ctggccggtg tgcacgatgt cttcgagaac cagttcgtgg aagccgaact gcgccccgat
     3001 acggcggatg agattcacgt cgctgacccc gtccacattc acccaggtga catgcccccg
     3061 gggaagcggc ccggaaggaa actgatcggc gtgcacttgc gtctcctcaa aatggccttt
     3121 gttgtacgag aagacatcga tcatctcgtt ggcattcggg ttgctcccga tgtacaccag
     3181 cgcttccggg gggctttgca gcgcccgccg ccggcttttc atgtgtcgcg acatggacat
     3241 ctccttaata tcgtccgctc attatattct tcagacggca ggcatgtcga acagaatccg
     3301 cgcggtcgat ttttgtgcgc ggttttcaat gatgcctatt gacgagcggc atccgtttcg
     3361 ggtagtttat ccgcagagtt gcagtgaatt ggacgggcat tgtgtgcgct tggacggacg
     3421 gaatgccgcg ggtgattaat caggagacag aaatgacata catcagaaaa ttgatgaaac
     3481 gaaaagggtt cacgctgatc gaactgctcg tggtgattgc gattatcgcc gtgctggccg
     3541 gtctgctgct gcccgcgatt tccaacgccc gcgagcgcgc caaccgcatt tcgtgcgtga
     3601 acaacctcaa gcagttcggc accggtctgt acctgtatga ggacagctac ggtgaaaaca
     3661 actggccggc gggccccggg cccacaaacg acattcgcgc gctgggcaag tacgccaatg
     3721 ctcccaagat gtacatctgc aagagcgaca agtatcgttc tgccgcggcg cgaattgagg
     3781 acataacggg ggccaactgc agctatatct acctcgccgg ttatcaggct tccgacaatg
     3841 gcaattttgt tgtcatgttt gacaagaacg gccgtgagga gccctccggc atccttgctg
     3901 aagacgccaa acttctcgga gctacaactc cgcccagcca ggccaacagc tggggtggaa
     3961 atcatggcac cgagggcggc aatgcgctgc atgtggatgg acatgccgag tggattcccg
     4021 tggtcagcga tgatttcaac atctcggacc ttttgaatga aatgcgcttc ccgtcgaacg
     4081 ccgtctatga actgacagtg acgacctgcg cggaggtcat tccggccaag taaccggaat
     4141 tcaggaatat agtgaagaaa gcgggaacat gtgttcccgc ttttttcttt gttttggagg
     4201 gtcgagctct gcgagatcga atttccgcgg cgtcgcagag ctccgccctc caatcctgaa
     4261 atagagcgtc tatatgaaca tctggagggt cgagctccgc gagaccgaat caccaccgca
     4321 acacattgat ttcgccgcaa aagggccagt catcgggtga ttcaaccaaa cctgcccgga
     4381 cgggattttg gcgcacatat tcccatttat tgccgtaact ctcttcgcgc cgtaattgag
     4441 tatcccaaaa ctcccgctgc catattggtt tttcattttt gcgcggccat tggcgggtca
     4501 cctcattctt ccaataagtg atccatgcct tcagccggca attcaaatct ctcggcgcgc
     4561 agaaaaggtg aatatggtcg ggcataatca catagcgacc gaccagccag cgatcggctt
     4621 ttgtccagcc atccaatagc aggtgttgtg cttctgtatt ggcaagcaaa ggtcgtttgt
     4681 ccttcacgca gagtgtcaga aacaaaatgg tcggatgatt gaccctgatg tgaggcggat
     4741 gatgcgcggg gtgacatctt tgcggcaatt ggctttcgtt catatccgtt tatgtacctc
     4801 tgcacatgaa aaagtgcgag ttcaatttcc gcggcgtcgc ggagctccgc cctccaaaaa
     4861 taccttccag cctccggtat gaacacatag ggggtcgatc tcccaccttc gccagggggc
     4921 ttcggaggac aagccgcgag accgtacttg ttttccatac tcctctgatt tagagtcatt
     4981 gccggaaatt cacagggata ctgatttatg tgcgggatca tcggatatgt tggcaatgag
     5041 gatgcgcagc ccgttattct ggacggcctg aagcggctgg aataccgggg ttatgactcc
     5101 gccggcctgg ccgtgctttc cggaggaacg ctcaccatcc gcaaacgcgt cggccggttg
     5161 tccagcctgg aacagacctg cgcggaaaac ccggtgcagg ggagtcccgg cattggccac
     5221 acgcgatggg ccacccatgg agaaccgtcc gaagtcaatt cccatcccca caccgacacg
     5281 gccggggaga tcgccgtggt ccacaacggc atcattgaga actatcttga gctgcgggag
     5341 atgcttgaga aagaaggctt tgttttcagg tcgcagacag atacggaagt gattccaaac
     5401 ctgattgcgc ttgaactgcg ccgggaccaa aaggatttca tgggcgctct atcgcgtgcg
     5461 ctgcagcgtg cgcgcggggc ctatgcgctg ggcatcctct ggacccgtga acccggccgc
     5521 atttatgcgg cgcggcaggg aagcccgctg atcattggtg tgggagaggg cgaaaatttc
     5581 attgcgagcg acgtgcccgc catcatcaac cgcgtgacac gcgtgattta tctcaacgac
     5641 ggcgagatgg cctgccttga ggccgatggg atcaggctgt tcaatctcaa gggcgaagaa
     5701 ctcaaaccgg acatccagaa ggttacgatt catgccgaag cggccagcaa ggcgggcttc
     5761 gagaccttca tgctcaagga aatccacgag cagccccggg tacttcacgg cctgctgtcg
     5821 aagcacacat ccgaccgtca ggacatcgcc gtggacctgt ccggactggg ccgggactac
     5881 tgccgcgcca tcaaccgggt gatgattgtg agctgcggca cggcctatta tgccggatgc
     5941 tacggccgtc ttctgctgga gcttctgctt gatatcaatg tggaagttga actgggaagt
     6001 gaattgcgct accgcagggc aaaactggat cccggcacgc tgatcatagc cgtttcgcag
     6061 tctggcgaaa ccgccgacac cattgccgcc gtgaaaaagg ccaaggcgtc cggctgcagg
     6121 gttctgtcca tctgcaatgt gcccggcagc tcgcttgtgc gcgaaagtca tgcggtgctg
     6181 ttcaccgatg cgggccccga aatcggcgtg gcttcgacga aggccttcac ggcgcagctg
     6241 atggccttcg ccctgttttc gctgggtctg gcccggcttc ggggagagct ttcggacgag
     6301 aaactgtcgc gctgcctggc cgagctgcag aaggtgccga cacacattca ttatgtgctc
     6361 agccgcaaag atgcgatccg ccagatagcc gacaagcatc atgacgggcc cagcgcgctg
     6421 tatctcggcc gccgcttcaa ctatcctatc gcgctggagg gggcgctgaa aaacaaggaa
     6481 atatcctatc agcatgcgga gggatatgcc gccggggaaa tgaagcacgg gccgattgcg
     6541 ctgatcagtg agtatctgcc ggtggtgtgc atctgcacga aaaccgatga cgagctctac
     6601 gagaagatga tgtccaatgt ccgcgaggtg gacgcgcgtc acgggcgcat tatcgccgtg
     6661 gccacggatg gcgatgaagc tgtccgcggc ctcgccgggg atatcatcta tgttccggaa
     6721 atcgccgaag agttttctcc catggtcaat gtcgtggcgc ttcagcttct ggcgtattat
     6781 tccgctcagg cccgcgggac ggacatagac aagccccgca acctggccaa aagtgtaacc
     6841 gtggagtgac cgggatggcg cgtttcataa tcaggggcgg caatgcggtc ggagggacca
     6901 tgcggcccgt cggcaacaag aatgccgcgc ttccgatgat cgcggcgtcg ctgctgacgg
     6961 atgaacccgt caggctgtcc aatgtcccgc gaattgagga cgtgcgcgtg atgctcgatc
     7021 tgctgcgggg cactggcgcg gaagtttcgc agcgcggcgg caccgtgacc atcgtttcgg
     7081 gaggcgggag ttccggtccg ctgaatcgcg tcctttgcac aaaagtgcgc agttcaatac
     7141 tgctggcggg gccgctggcg gcccgccggg gccgggtggt gctggcgccg cccggcggcg
     7201 acgtgatcgg ccgccgccga ctggacactc atttttatgc cttgcgcgcc cttggaattg
     7261 atgtccggat cggatcctcc gtgacattcc agaggaaaaa actggccggc gcggaaatcc
     7321 tgctggacga agccagcgtt acggccacgg agaacgcgat gatggccgcc gtgctggcgc
     7381 cgggcagaac ggtgatttac aatgccgcct gcgaaccgca cgttcaggac ctgggcgtga
     7441 tgctggtcaa aatgggggcc cgcatcgagg gactcggcac caaccggatt gaaatccggg
     7501 gcgtcaggcg cctgcatggg gccgcgcatc gcgtgggtgc ggactatatc gaaatcggca
     7561 gcttcatcgc cgcggccgcc gcaacgggcg gcgcgctgga catcccgctt gatggagaca
     7621 gccgggtgtg gcttcctgtc gtcgagaaga tgtttcagac catgggcatt tcgtggtcgg
     7681 tgcggaatgg aaagctgcat atgcccgcgc ggcaggagaa ggccgttcag tcggcggcgg
     7741 cgtctgatat tccgacgatc agcgacggca tctggcccgc cttcccgtcc gacctgatga
     7801 gcgtggcgat cgtgctggcc acgcagtctt ccggcacggc gctctttttt gaaaagcttt
     7861 ttgagagccg gatgtatttt gtggatcatc tcatcagcat gggcgcgcgc attgtccagt
     7921 gcgatccgca ccgcgtggtg gtggtcggtc cttcccggct gcgtccggcc cacatgagtt
     7981 ctccggacat ccgcgccggc atggctctgc tgatcgcggc gctgtgcgcc cgcggcgaga
     8041 gtgtcattga aaatgcgcag atcatagatc gcggctatga gcgcattgag gaaaagcttc
     8101 gcgcccttgg cgcggacatt cggcgcgtgg aatagcggcg cgccgccgga ttgacgccgc
     8161 agaatctgaa cggtagattt ccctccgaag catttcattt gatgaaggct ttccacagtt
     8221 cggatcgcaa cagcctggcc gaaattttca gccgggcagg gggactgttt gtgcccgggc
     8281 tggacggcgc ggcggccgca tatttcgccg gcgcgctttt tcatgacttc ccgtgctcgt
     8341 ggctgtgcat cacggatggt ccccgcacgc aggagacttg tcaccgcgac ctgcagtcgc
     8401 tttttcccgg gcatgcgaag cgcatcctga actatcccgc gtgggaggcg tatccgaacc
     8461 tcgcccccga aatcaccggc gaacgcctcc gcgcccagca ggcccttgtt gaaaattccg
     8521 attcatgcct tgtcgtgagc tgcatccagg cgctgatgca gccgactctt tccccgcagg
     8581 aacttgaaag gacggtgatc cccttgagaa agggcgatca ggcagaccct gcggatttgg
     8641 cgtcgaagct gctggcggcc ggctatcgct tctcgcacga ggtttttgag cgcgggcagg
     8701 catcgcttcg cggcggcatt ctcgatgtct ggccgcccga cagcgaatat cccgtgaggc
     8761 tggatttctt tggcgacacg ctggattcca ttcgccattt caacgccgag gaacagacct
     8821 ccatcgcaaa aatcgaatcc gtgcggctgt cgccggccat ggaaaatatg gcggttcagt
     8881 ttccggaccc gaccgcgagc ctgctttcgt attttcctga aaaatacggt gtggtatttt
     8941 tcagccagaa ccggattcgc gagcacgggc ggctgcttga agattccatg aaggcggaga
     9001 ttgggggccc cggtctggat gccgttctgc agcgggttga aaaaaaggcg gcccatgccg
     9061 tttatctggg acagggccgc ccgccgcgga cgccgcaatg ggagtcgccg ttccatcccg
     9121 tggagagcct gccgcgaccg gccgggaccg attacctgcc ggacatcatg gaaaaggccc
     9181 ggcgcgaata tgtcgaaggc ctccttcaga aaacggaaca gggctcggaa gtccgtgttt
     9241 tcttcgatac cgcgggaacg cgcgaacgct ttctcacgat ggatccccgg cacgcgcgct
     9301 tcaagagctg cgtcgcgccg ctctcggcgg ggttcagtta tccggcgcag agtctgatgg
     9361 ccatca
//
LOCUS       DAOQWR010000103         9363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7846_length_9363_cov_4.720241, whole genome shotgun sequence.
ACCESSION   DAOQWR010000103 DAOQWR010000000
VERSION     DAOQWR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7846_length_9363_cov_4.720241"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKK36_08585"
     CDS             <1..533
                     /locus_tag="PKK36_08585"
                     /inference="COORDINATES: protein motif:HMM:NF020129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNR94649.1"
                     /translation="YHLPFTRMAEKAHTSLVRQASPDFPEGRAAAQIEAALCYNRIIG
                     NTYTGSLYIGLTSLLEHEADLTRQRLGLFSYGSGCLAVFFGGVLQEGYLAHLDRERHR
                     AMLDGRRELSMQEYEKLYSQRLPKDGSDLQFSPHTRGLFRLQGISAHKRLYSRNAPLA
                     EESAERRSSVRTHAVC"
     gene            complement(611..4753)
                     /locus_tag="PKK36_08590"
     CDS             complement(611..4753)
                     /locus_tag="PKK36_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009751372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNR94650.1"
                     /translation="MIKKVMVFGLLMLIAGTSSAVQIRVATYNVEYGFDGPGSEEWVA
                     ITSMLTRVGADIVGMNELTTSAYDNFIMMAAYLGYPYTAFSTNYNAMTGGSRLGYMSR
                     FPIISSAAIPQYPGALEVARPPLRCVFDVPGALNNFVAYEVHYKASQTDPDEFQRAIE
                     VRRTRNHIIEYMTNELTKLDTEFVVMGDMNDDIDEYPGQTTSFSSMPSGMNDSFVLGS
                     DVDFPVAYRVFPNDRFSEDIDFLTLHPYREDSTEDGTSWQSDIRWDYMIFSRDIMESP
                     YGAPMGEVYHSKWDDGAGTGGLPKYGYPLNNWIISNGPNHCIVFADFHLMDLLPCLNP
                     VLLISEIVDSTVSTNANYVELYNSGTTALSLTNYQLVIYIDGSSPATVSLTGNLAPGG
                     KYVIASHSNEFVSTYPGKFPTQISTWVNRLNGNDVVAIKNPNNMLTDLFGGIGEPTSG
                     SDYSMAWAYNNKRAERIFGISDPSADFITNEWTIGTSSPTPGVHSACDEASIYYANIE
                     MDPFAPATGVSFVVSADIQPNLPASNLAPTVYYRLNSDSWSSIGMTNVSGTVWAAPAI
                     NPGAEGDDNLYYYILTSFDGPSAVPSPSATNTYKYPPKPGSAGDTPRFNEIEFNSAGT
                     DGTNPVEFIEIIAPAGMNMSGYYMVHYNGGTSDGGEWTWVFPSFVVPDDSVTDVYNTA
                     LGFVVVGPTGCTIPTVDFASLTNISSVTGILQGDGDGLVLYDSAGNVMDAIGWGAGFG
                     DMMTDDPGPSVLSTNPPTSADNYLHDLNADTYNDDRSVQAPNDVLSDDGTSWARATAT
                     PGAINSGQTSGSITIGVPAGTDEDGDGIGSYTDNCPDDYNPTQSDIDGDGIGNACDDD
                     RDGDGVANASDNCPDTPNADQSDVDSDGTGDVCDYDNDNDGVDDEIDNCIFTWNPGQE
                     DTDDDGIGDACDEDWDNDGTTNAVDNCPTTSNADQSDLDSDGIGDVCDPDIDGDGVNN
                     DVDNCPLTYNPTQTDSNSDGIGDVCSNDSDLDGVDDSLDNCPGTYNPDQADDDNDGLG
                     NACDDCTGVYVTNLLASAEFETGQPAGWTVRTNAGANAAWRFDNPKFKENYTGGTGLF
                     AVCQSDMFRKTDMDTELLTPSMDMGLALIADLQFKTDFDWDRFGDNEKCDVDVSLNGS
                     AGPWSNVWRKQGADYRGPATEKVDISFAAGSTNVVIRFRYYDAYQEYWWQVDDVKVIC
                     ELCDADHDSDGDGIPDISDNCPDVWNPTQADIDGDDAGDDCDDDIDGDGIVNDWESLY
                     EMDPYDDSDALLDDDSDGMINLYEYQADTSPTNFNSVLRISNPRYTKSTDRFGISFPS
                     STSRIYRVEFNDGNVGNSNDWYVGIAPFTGKVGSSTVTDSTVNAVSPGAIRYYRVRAE
                     VP"
     gene            complement(4937..6010)
                     /locus_tag="PKK36_08595"
     CDS             complement(4937..6010)
                     /locus_tag="PKK36_08595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94651.1"
                     /translation="MKSIHQFVAGFSHGDAISNETLMLRDIFRSWGYQSEIFSEAKRI
                     LPELRREAHDAAAFAANCHSDDVVLLHLSIGSPVNRIFAELPCKKAILYHNITPPEFL
                     QGIQEQIASHLARGREEMKMLAGTAQVVMADSRFNAEELESMGYGKAEVLPLVLDLDK
                     LKARPHRPTVRKYADGLVNVIFVGRCVPNKRIEDVLRAFYYFQKYVEPHSRLIHAGSF
                     AGTEQYHALLLTQARDLGLRNMDLVGSIPQDELNAVYSVASMFLCMSEHEGFCIPLIE
                     SMVHDVPVIAYAAAAVPETMDGAGILIKEKRFDLIAEMMGRVLHDEALRRAVIEGQRQ
                     RLQRYRSRDLAVELKSHLGPLLA"
     gene            complement(6007..7203)
                     /locus_tag="PKK36_08600"
     CDS             complement(6007..7203)
                     /locus_tag="PKK36_08600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94652.1"
                     /translation="MKPNAHIAFVSPRFAAGATLGGAETLLRKLAEHAAERGCRVTFL
                     ATCAENHFTWENSIPAGSKMIGDLEVVYFPVDSNRNTGDFLRAQQAISAGKPVSIEEE
                     KLWMKNSVHSRAMYDHLKKEGEKYDAVITGPYLFGITYAVSRIWPRKTLLVPCLHDEA
                     FAYLQIMRTMFEETAGCLFNSEPEKFLAQKIFGIPEARCRVVGMGMDDFECSPTEFAR
                     RHKIDVPYLLYSGRREPLKGTPLLLDYVNAFRLRTGRNIMLILTGSGDFQRPPELEGH
                     IFDAGFVSEREKHDAMAGALAFCHPSVNESFGIVLLESWLAGTPGLVHARSEVLKYQC
                     QKSGGGLWFKSYPEFEEELLLLLDNPALREKMGAAGRKYVQETYSWEKVGQRFFEGLD
                     SLVRNI"
     gene            complement(7218..7658)
                     /locus_tag="PKK36_08605"
     CDS             complement(7218..7658)
                     /locus_tag="PKK36_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94653.1"
                     /translation="MSSAAFEIHAPGVDTEKIVREIEAAVAKKQEEGFYSDARVARAE
                     RSNLAHLRNEEQFLGFYLKCLRDAVFVDISDFEIRERRRGLAAPLVILKKTIWKLLKF
                     YTYRLWSQQNEVNGLLVTAVQGLEEQYSSRIKALEKRIEELEKK"
     gene            7797..8072
                     /locus_tag="PKK36_08610"
     CDS             7797..8072
                     /locus_tag="PKK36_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94654.1"
                     /translation="MHSHQDPKPIGKCGGCPLNLKKSCGMFEHPAEQWAHGRCKGFMN
                     AKMFEAFVAAQAREHEKTHKEIRREKQEERKSISHVDGIPNPGGSRW"
     gene            complement(8267..9100)
                     /locus_tag="PKK36_08615"
     CDS             complement(8267..9100)
                     /locus_tag="PKK36_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94655.1"
                     /translation="MEQLLSIDGKYVIPRIKKGDWTMKMVKFGLTMFLAAAVCGLCAC
                     QAKTETAQVPAPEVLETAQAAAVEAEAVVEETLDTALEAAAGAVESTVQAVEEVAEDI
                     PSMAMDELLPAEEDVEEAAMDSDDIELVPAGEDFPAVEEIIEEIPVAVEEVIEEPLPE
                     VEESVMDDISSMDETIPAFEEAVEEMVADEAAFMAEEVIEEIPSIEEAPAIAEEMSSE
                     AAVPAAETVEIAPAMAEEAFVEEITPAVEMIEEVPAMIDEPVLEEVAPVVEEEVPALA
                     E"
     gene            complement(9169..>9363)
                     /locus_tag="PKK36_08620"
     CDS             complement(9169..>9363)
                     /locus_tag="PKK36_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94656.1"
                     /translation="QPKVRDDSNRKYDLLPGVRRLVLDQQGGDRAWRGDADIIEGHLT
                     IINIRPHADPDRFDISVRFD"
BASE COUNT         1939 a         2619 c         2681 g         2124 t
ORIGIN      
        1 gctatcatct gccgttcacg cggatggccg agaaggcgca taccagtctg gtccggcagg
       61 cctctcctga ttttcccgaa ggccgggcgg ccgcgcagat cgaagcggca ctgtgctaca
      121 accgtattat cggcaacacc tacaccggct cgctgtatat cggtctcacc tcgcttcttg
      181 aacatgaggc ggacttgacc aggcagcggc tgggcctgtt cagctacggg tcgggatgcc
      241 tcgcggtatt cttcggcggg gtgctgcagg agggatatct ggctcatctg gaccgggagc
      301 gtcaccgggc gatgctggac ggccgccgtg aattgagcat gcaggaatac gaaaaactat
      361 attctcagcg cctgccgaag gacggttcag atctgcagtt ttcgccccat acgcggggac
      421 tgttccgcct gcagggcatc agcgcgcaca agcggctgta cagccgaaac gcaccgttgg
      481 ccgaggagtc tgcggagcgt cgatcctctg ttcgcaccca tgcggtgtgc tgaggggcga
      541 acagttcagc gcaatgaaaa aaaaccgccg cggtttcccg cggcggtttt ctttcgatct
      601 gagcctgtac tcagggaact tccgcgcgga cgcgatagta ccggatggcc ccgggagaca
      661 cggcattgac ggttgagtcg gtcaccgttg aagatccaac cttgcccgtg aacggggcaa
      721 tgcccacata ccagtcgttg gaattcccca catttccgtc gttgaattct acgcggtaaa
      781 tccgcgatgt gctggacggg aatgaaatgc cgaaccggtc tgttgatttt gtgtagcgcg
      841 gattcgatat ccgcagcacg ctgttgaagt tcgtgggact ggtgtccgcc tgatattcgt
      901 aaagattgat cattccgtcg ctgtcgtcgt caagcagggc atcggaatca tcatagggat
      961 ccatttcgta cagggattcc cagtcgttca caataccgtc gccgtcaata tcgtcatcgc
     1021 agtcatcgcc cgcatcatcg ccgtcgatat ccgcctgtgt gggattccag acatcggggc
     1081 agttgtcgga tatgtcggga atgccgtcac cgtcgctgtc atggtcggca tcgcaaagct
     1141 cgcatatcac cttcacatca tccacctgcc accagtattc ctgataggca tcatagtagc
     1201 ggaaacggat aacgacattt gtagagccgg ctgcaaaaga gatatcaact ttctcggtgg
     1261 ccggtccgcg gtagtccgcg ccctgcttgc gccagacgtt ggaccatggc ccggccgacc
     1321 cgttcagact gacatccaca tcgcattttt cgttatcacc gaacctgtcc caatcaaaat
     1381 cggtcttgaa ctgcaggtcg gcgatcaagg caaggcccat gtccatagag ggcgtcagca
     1441 gttcggtgtc catatccgtt ttcctgaaca tatcgctttg gcagaccgcg aacaggcccg
     1501 ttccgcccgt ataattctcc ttgaatttag gattgtcgaa acgccatgcg gcattggcgc
     1561 cggcatttgt gcgcacggtc cagccggccg gctggcccgt ctcgaattcg gcactggcca
     1621 gaagattggt gacatagacc ccggtgcagt cgtcacacgc atttccaaga ccgtcgttgt
     1681 catcatccgc ctgatcgggg ttgtaggtgc cggggcagtt gtccagcgag tcgtccacgc
     1741 catccaggtc gctgtcattg gagcagacgt cgccgatccc atcactgttg gaatcggttt
     1801 gagtcgggtt gtaggtcagc gggcagttgt ccacgtcgtt gttgacgccg tcgccgtcta
     1861 tatcgggatc gcatacatcg ccgatgccgt cggaatcaag gtcagactga tccgcattgg
     1921 aggtggttgg gcagttgtca acggcgttgg ttgtgccgtc attgtcccag tcctcgtcgc
     1981 aggcatcgcc gattccgtcg tcgtctgtat cttcctgccc tggattccac gtgaagatgc
     2041 agttgtcaat ttcatcatcc acgccgtcgt tgtcgttgtc atagtcgcac acatcgccgg
     2101 tgccgtcact gtccacatcc gactgatcgg cattgggcgt gtccgggcag ttgtcggaag
     2161 cattggccac accgtcaccg tcccggtcat cgtcgcaggc atttccgatg ccgtcgccgt
     2221 caatatcaga ctgtgtcgga ttgtagtcgt ccgggcagtt gtccgtgtag gatccaatcc
     2281 cgtctccgtc ctcatccgtg cctgccggca cgccgatggt gatgcttcct gaggtctgac
     2341 cgctgttgat cgcgcccggg gtggccgtgg ccctggccca gctggtcccg tcatcagaga
     2401 gaacatcatt gggcgcttgc acggatctgt cgtcgttgta cgtgtctgca ttgaggtcgt
     2461 gcaggtaatt atccgccgat gtcggtgggt tggtgctcag caccgagggg cccggatcgt
     2521 cggtcatcat gtcgccgaag cccgctcccc atccgatggc atccatcaca ttgccggccg
     2581 aatcgtagag gaccagcccg tcaccatcgc cctgaagaat gcccgtcacg gatgagatgt
     2641 ttgtcaggct ggcgaaatcc acggtgggaa tggtgcagcc ggtcgggccg accacaacaa
     2701 atccgagcgc cgtgttgtag acatcggtaa cactgtcatc tggaacaacg aatgacggga
     2761 acacccatgt ccattcaccg ccgtcagaag ttcctccgtt gtaatgcacc atgtaatatc
     2821 cgctcatgtt catgccggcc ggagcgatga tttcaatgaa ttcaaccgga ttggtgccgt
     2881 ctgtgcccgc cgaattgaac tcgatttcat tgaagcgggg cgtgtctcct gcgcttcctg
     2941 gtttgggcgg atacttgtag gtgttggtgg ccgaagggga ggggactgcc gaaggaccgt
     3001 caaaggaggt caggatgtag taatacaaat tgtcatcgcc ttcagctccg ggattgatgg
     3061 ccggggccgc ccagactgtg ccggacacat tggtcatgcc gatcgatgac caggaatcgc
     3121 tgttcaggcg gtaatagacg gtgggtgcaa gattcgatgc cggcaggttg ggctggatgt
     3181 ccgcggacac cacgaaggag accccggtcg cgggagcgaa cggatccatc tctatgttgg
     3241 catagtaaat actggcctcg tcgcaggcgc tgtgaacccc gggcgttggg ctgctggtgc
     3301 cgatggtcca ctcgtttgtt atgaaatcgg ccgacgggtc actgatcccg aaaatgcgct
     3361 cggcccgctt gttgttatag gcccaggcca tgctgtagtc ggacccgctg gtgggttcgc
     3421 cgatgccccc gaagaggtcg gtcagcatgt tgttgggatt cttgatggcc accacatcgt
     3481 tgccgttcag gcggttcacc caggtgctga tctgagtggg aaatttcccg gggtaggtgc
     3541 tgacaaattc gttggaatga gacgcaatga cgtatttccc tccgggggcg aggttccccg
     3601 tcagggaaac ggtggccgga gaggagccgt caatgtagat gaccagctga taattggtca
     3661 ggctgagggc tgtggtgccg gagttgtaga gttcgacgta attggcgttg gtgctgaccg
     3721 tgctgtccac tatttcgctg atgaggagca cgggattgag gcaggggagc aggtccatca
     3781 ggtggaagtc ggcgaagacg atgcagtggt tggggccgtt gctgatgatc cagttgttca
     3841 gtggatagcc gtacttcgga agcccgccgg tgcctgcgcc atcatcccat ttcgagtgat
     3901 acacttctcc catgggcgcg ccgtaggggc tttccatgat gtcgcggctg aagatcatgt
     3961 aatcccatcg aatatccgac tgccagcttg ttccgtcctc cgttgaatcc tcgcggtacg
     4021 ggtggagtgt caggaagtca atgtcttctg aaaaccggtc gtttgggaaa accctgtagg
     4081 caaccgggaa gtctacgtcg ctgcccagca caaaggaatc gttcatgccg ctgggcatgg
     4141 agctgaagga tgtggtttgc cccggatatt cgtcaatatc atcgttcatg tcgcccatca
     4201 caacgaattc cgtgtccagt ttagtgagtt cattggtcat gtattcgata atgtggttac
     4261 gggttcggcg gacttcaatg gcgcgctgga attcgtcggg atcggtctgg ctggccttgt
     4321 aatgcacctc gtacgcgacg aaattgttca atgcgccggg cacatcaaac acgcatcgga
     4381 gcggggggcg cgcgacttcc agtgcgccgg gatactgggg gattgccgcg gaagagatga
     4441 tggggaagcg gctcatgtaa cccaggcggc ttccgcccgt catggcgttg tagtttgtcg
     4501 aaaatgcggt gtagggatag cccagatagg cggccatcat gatgaaatta tcatacgcgc
     4561 tggttgtcag ttcattcatg ccgacgatgt cggctccaac gcgagtcagc atgctggtga
     4621 tggcgaccca ttcttcagat ccgggcccgt caaaaccgta ctcgacgtta tatgttgcca
     4681 cccgaatctg cacggcactg ctggtgccgg cgatcagcat gagcaaacca aataccatga
     4741 cttttttaat catcggggat tctcccttaa gggctgtcat tctctgataa cagcgaacga
     4801 tgaaaacata ccccgcgtga gagaaaagtt tagtcggaaa cagcagaaaa tgggcgaaaa
     4861 gttgagattc cgggggcaca acacaatggg ggtacacgga cttatatccc cccagatatt
     4921 gtgcgttcga ggacctctat gccagaagcg ggccaagatg gcttttcagt tccactgcga
     4981 gatcgcggct gcggtagcgc tggagccgct gccgctggcc ctcgatgacg gcccggcgaa
     5041 gggcttcgtc atgcaggaca cggcccatca tttccgcgat aagatcaaag cgtttttctt
     5101 taatcagaat gccggcccca tccatggttt ccgggacagc ggccgccgca taggcgatga
     5161 cgggaacgtc gtgaaccatg ctttcgatca gggggatgca gaaaccctca tgctcgctca
     5221 tgcagagaaa catgcttgcg acggaataaa cggcattcag ttcgtcctgc gggatggacc
     5281 ccacaaggtc catgtttctc aggcccagat cgcgcgcctg tgtcagcagc agggcgtgat
     5341 actgctcagt gccggcgaaa gagcccgcat gaatcaggcg cgaatgcggt tccacgtatt
     5401 tctggaaata ataaaaggcg cgcaggacgt cttcaattcg cttgttgggc acacagcgcc
     5461 ccacgaagat gacattcacc agcccgtctg catacttcct gacggtgggc cggtgaggcc
     5521 tggccttcag tttgtccaga tccagcacaa gcggcaggac ttctgctttt ccgtagccca
     5581 ttgattccag ttcttcggcg ttgaagcggc tgtcggccat gaccacctgc gctgtgcccg
     5641 ccagcatttt catttcctcc ctgccgcgcg ccagatgtga tgcgatctgc tcctgaatgc
     5701 cctgcagaaa ttccggaggg gtgatgttgt gatagagaat tgcttttttg cagggaagtt
     5761 ccgcgaatat ccggtttaca ggcgatccga tggaaagatg cagaagcacc acgtcatccg
     5821 aatggcagtt ggcggcaaag gcggccgcgt cgtgcgcttc cctccgaagc tccggcagga
     5881 tgcgcttggc ctccgagaag atttccgact gatatcccca cgaccggaag atgtccctca
     5941 gcatcagcgt ttcattgctg atggcatccc cgtgactgaa ccccgcgaca aattgatgta
     6001 tggatttcat atatttctga ccagcgaatc aaggccttca aagaatctct ggccgacttt
     6061 ttcccatgag taggtttcct gaacatattt ccgtcccgcc gcgcccattt tttcgcgcag
     6121 ggccgggttg tcgagcagga gcagcagttc ctcctcgaat tcgggatagg acttgaacca
     6181 caaaccgccg ccgcttttct ggcactgata tttcagcacc tcgcttcgcg catgcaccag
     6241 ccccggtgtt cccgccagcc acgactccag cagcacaatt ccgaaactct cattcaccga
     6301 aggatggcag aaggccagcg ccccggccat ggcatcgtgt ttttcccgtt cgcttacaaa
     6361 gccggcatca aaaatgtgcc cctccagctc cggcggtctc tggaaatcgc cgcttccggt
     6421 caggatcagc atgatattgc ggcctgtgcg cagacggaag gcgttgacat aatcgagcag
     6481 aagcggcgtg cccttcagcg gttccctccg ccccgaatag agcaggtagg ggacatcaat
     6541 cttgtgccgc cgggcgaatt cggtcgggct gcattcgaaa tcgtccatgc ccatgccgac
     6601 cacgcggcac ctggcttccg gaattccgaa gatcttctgc gcgaggaatt tttccggttc
     6661 cgagttgaac aggcatccgg ccgtctcttc aaacatggtc cgcatgatct ggagataggc
     6721 gaaggcctcg tcgtgcaggc acggcaccag aagcgtcttg cgcggccaga ttcggctgac
     6781 ggcataggtg atcccaaaaa gatacgggcc tgtgatgacg gcgtcgtatt tctcgccctc
     6841 ctttttcagg tgatcgtaca tcgcccggct atggacgctg tttttcatcc acagcttctc
     6901 ttcctcgatg gacaccggct tgccggcgct gatggcctgc tgggcccgca ggaaatcccc
     6961 cgtattgcgg tttgagtcca cgggaaaata gacgacctca aggtccccaa tcatttttga
     7021 cccggccggg atcgagttct cccatgtaaa atggttctcc gcgcaggtcg ccaggaaggt
     7081 cacccggcag ccgcgttcgg cggcgtgctc ggccagtttg cgcagcagcg tctccgcgcc
     7141 gccgagcgtg gcgccggccg caaagcgggg cgaaacaaag gcgatatgcg cgtttggctt
     7201 catggatgcg gaacagccta ttttttctcc agctcttcga tccgtttttc aagggccttg
     7261 attctggagg agtactgctc ctccaggccc tgcacggcgg tcaccagtag tccgttgacc
     7321 tcgttctgct gcgaccagag acggtaagta tagaatttca gcagcttcca gatggtcttt
     7381 ttcaggatga ccagcggtgc cgcaagcccc cgcctgcgtt cgcgtatctc aaagtcggag
     7441 atgtccacaa agacggcatc ccggagacat ttgagataga atccaagaaa ctgctcctcg
     7501 ttgcgcagat gggcaaggtt cgaccgctcg gcgcgggcta cccgggcgtc ggaatagaat
     7561 ccctcctcct gcttcttcgc caccgccgct tcgatctcgc gcacgatctt ttccgtgtcc
     7621 actccgggtg catggatttc aaaggccgca ctgctcataa aacattcctt tcaaagtgtc
     7681 cgcattattt gaccattgac cggcagtgtc aatgccggat gaaaggtttg acccgggcag
     7741 gcggggatgg taccctgtaa tcagagaggc ggtacggata caggaaggag gctggtatgc
     7801 acagtcatca ggatcccaag ccgattggga aatgcggcgg atgtccgctg aatttgaaaa
     7861 aaagctgcgg catgttcgag catccggctg aacagtgggc gcacggccgg tgcaagggct
     7921 tcatgaacgc caaaatgttc gaagcctttg ttgcggcgca ggcccgggag catgagaaga
     7981 cgcacaagga gattcggcgc gaaaaacagg aggagaggaa gtccatttct catgtggacg
     8041 gcattccgaa tcccggggga agccgctggt aaaaaatgcc ggaaattggc ccaaaatggc
     8101 caaaaagggc ccaaaaacgt caaaatagtg aaaaaaatac cgaaaacgtg attttttgga
     8161 aatagtacta aaatagtact atttattgcc ggggaactgt tcatgacctg caaaaaaaac
     8221 aggaaggacc tgcgtccttc ctgttttctg taaaaagaca aactgattat tcggcgaggg
     8281 ccgggacttc ttcttcgaca accggagcga cctcttcgag gacgggctca tcaatcatgg
     8341 ccgggacttc ttcgatcatc tcgactgccg gggtaatttc ttcaacaaag gcttcttcag
     8401 ccatagccgg agcaatttcg accgtctcgg cagccgggac ggcggcttca ctgctcattt
     8461 cttctgcgat ggccggagct tcctcgattg acgggatttc ctcgatcact tcctcagcca
     8521 tgaacgcggc ttcgtcagcg accatctcct caacggcctc ttcgaaagcc gggatggttt
     8581 catccatgga tgagatgtcg tccatgacag attcttcaac ttcaggaagg ggctcttcaa
     8641 tgacttcttc gaccgctaca gggatttcct cgattatttc ttcaaccgcc gggaaatctt
     8701 caccagccgg aactaactcg atgtcatctg aatccatggc ggcttcttcg acatcttctt
     8761 ctgcgggaag cagttcatcc atcgccattg aagggatatc ttcagcgact tcctccactg
     8821 cctgaaccgt gctctccacc gctccggccg ctgcctcaag agcggtatcc aaggtctctt
     8881 caacaacggc ttccgcctca acagccgcgg cttgcgcggt ttccagcact tccggagcgg
     8941 gaacctgagc agtttccgtc tttgcctgac aggcgcacaa tccgcagacc gccgccgcta
     9001 aaaacatcgt caaaccgaat ttcaccattt tcatagtcca atcacctttc tttattctgg
     9061 gaatcacgta tttgccgtca atcgacagca actgttccat tgggggaggg attcttgtga
     9121 aatatctgcg aaaagcaagc agaaaacgac aagtgtgcgc gcgagcaatc agtcgaaacg
     9181 cacagaaata tcgaagcggt cgggatcggc gtgaggccgg atgttaatga tggtcagatg
     9241 tccctcaatg atgtccgcat cgccgcgcca tgcgcggtct ccgccctgct gatcaagaac
     9301 gaggcgccgg acacccggca ggaggtcgta cttgcgattt gaatcatcgc ggactttggg
     9361 ctg
//
LOCUS       DAOQWR010000104         9293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_7917_length_9293_cov_4.426391, whole genome shotgun sequence.
ACCESSION   DAOQWR010000104 DAOQWR010000000
VERSION     DAOQWR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7917_length_9293_cov_4.426391"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1047
                     /locus_tag="PKK36_08625"
     CDS             181..1047
                     /locus_tag="PKK36_08625"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012083286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNR94657.1"
                     /translation="MLSKPVIIFGTGRSGTTVLHRVLSSHPEFAWMSGLCDRHPGAPQ
                     YNRSLMHALDFPVLGDFLKRKFPADEGYRFWDRCYRGFSTPCRDLLASDVTVRAKEDI
                     LKTMSKLITNRRARLLLKVTGWPRTGFLSEVFEGAKFIHVVRDGRAVVNSLLNVDFWR
                     GWGGPEKWRCGPLPPAYAEEWRRHSQSFVVLAAIQWKILMDAAEKAKQLLDSTRIMEI
                     KYEDICREPENSLKAIAGFCEVQWSDEYAARVRRFRFTSANDKYRNELTLQQQAELED
                     VLRPCLARCGYS"
     gene            complement(1038..1439)
                     /locus_tag="PKK36_08630"
     CDS             complement(1038..1439)
                     /locus_tag="PKK36_08630"
                     /inference="COORDINATES: protein motif:HMM:NF017482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNR94658.1"
                     /translation="MKTPESNPLRVNRYALPDGWEILAGKSDRDNDALSLIGAHPADW
                     WFHVKGMPGSHVILRHPEHLEPSRELLECAAATAAWHSKARNGGITAVSCTRAGRVSK
                     PRGAKAGTVHITKARVLKVRPACPDSREHHE"
     gene            complement(1420..1722)
                     /locus_tag="PKK36_08635"
     CDS             complement(1420..1722)
                     /locus_tag="PKK36_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNR94659.1"
                     /translation="MTAYQRFTSENLTLRDELAIDRTLLANERTLLAYLRSGVALILA
                     GVTLFHFSTARWFQIVGLTCVPAGVIVMLIGTLRFCRMNERIMTARKQRANEDAGK"
     gene            complement(1763..3334)
                     /locus_tag="PKK36_08640"
     CDS             complement(1763..3334)
                     /locus_tag="PKK36_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94660.1"
                     /translation="MAAETQYKILILEDRPEDCELVQRVLSREGIAFVMHRADTEKDM
                     LKELRQFKPDIIISDYVMPGFNALKALDIVLKAAPETPFIVVSGTVGEETAADAIRSG
                     ASDYVMKDNLKRLGPAIRRELDKIERRRRTQQVESDLRKRLQNAEQMETLGKMAGKVA
                     HDLNNIIGPLVAYPDLILDQLPPESPVRDDILEIRKAAEHAAEMIKDLLTLARRGNFH
                     AVSVDLNVVVKEYTKSLGLNMLLARHPEQKLECQLAENLPHIMGSAPHLQAVLMNLVI
                     NAIDAGKPKPVLISTKAVNLEHPLMGYEKIPAGHYVVLSVADKGHGIEPADMKRLFEP
                     FFSRKQMGSSGTGLGLAIIYGILKDHQGYIDIRSEVGIGTEFLLYFAAATETAQAAPP
                     AELPSYEGEETVLIVDDLQPQLDLARALLSSLGYHVLTAANEYQAMNILDENTVQLAL
                     LDMNMGGGGNGLHLFERILEKVPDLPCIFVSGSANQPAIEEARKMGCSAFVQKPFSRP
                     VLGRAVREELDRHMA"
     gene            complement(3340..3807)
                     /locus_tag="PKK36_08645"
     CDS             complement(3340..3807)
                     /locus_tag="PKK36_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94661.1"
                     /translation="MSDSACPVEILLVEDNPDDIELTLRALQTKNITNRIHVVRDGEE
                     ALEYVFCEKRYAGRDIHHPPKVILLDLKLPKVDGLTVLRHIKGDARTKAIPVVILTSS
                     EEERDVINTYNCGANSYLVKPIEFEQFINAISSLELYWLILNTPPPEGAFNGQ"
     gene            complement(3873..5150)
                     /locus_tag="PKK36_08650"
     CDS             complement(3873..5150)
                     /locus_tag="PKK36_08650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNR94662.1"
                     /translation="MKNRTHRNSDSGALRISRRVNLAMDVVEQVREQVADGRPADAAL
                     QAIFRKHREYGSRDRKLYANMVFSCFRWLGWLKGLAPVQVCAMAQMLEAGETSELLTA
                     LAEAAGFQAVPMAPLSLEQKARETGQMLGRELSVEALLPAGVLEHIPVPEGRRISFIG
                     SMQTRPPVWLRFADADTADRFSTGGAVIHPRLSEAAALPENFNRELLNTPEGRKAEVQ
                     DLSSQCVGLIAGPKRGERWWDVCAGAGGKALHLAALTEGLADILASDLRETSLNRLAG
                     RAERTGMRGLRTAVLDGRRDSPGLGGFDGVIVDAPCSGIGTWARNPDARWRFAPDMIS
                     RSAEVQKQILRNAARHLRPGGVLVYAVCTCTREETETVTSGFLSEHSGFEPADFTHPL
                     TGAPCPGACMIWPEAGPCNGMFIAKFQKLGDQG"
     gene            5210..6367
                     /locus_tag="PKK36_08655"
     CDS             5210..6367
                     /locus_tag="PKK36_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNR94663.1"
                     /translation="MARELAFVLINPYTIGKSRTGGVIGRYIRTGLDLVGARIFGPSK
                     ALTEKYAALIREDPEVDEDQRDILADYVLRTYMPDPQTGRRHRVFMLLFEGENAIERI
                     REATGKVRYNMASGRSVRDIYGDYVTSPAGEVLYVEPAVLIGPTLKSCAAALKLWAEY
                     SAKDGGIIADAFDLADDNIEKTLVLIKPDNFRFPSSRAGNIIDLFSGSGLRIVGAKLH
                     QMSVVEAEEFYGPVREFLRKKFKGSAGDQAYEAVAKKFGIEIPETMKEALGEMFGPLV
                     ADHQFYKIIEFMTGWWAPSCGIDGKDCPGKSKCLALVYAGENAIGKIRDILGPTDPSK
                     AQPGSVRKEFGSDVMVSAAHASDSPENAAREMKIIKVEKDTIKKLVQQYYP"
     gene            6392..7594
                     /locus_tag="PKK36_08660"
     CDS             6392..7594
                     /locus_tag="PKK36_08660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNR94664.1"
                     /translation="MFEEIQPAPADAILGLTEAYKKDPRPVKVNLGVGVYQDEQGHTP
                     ILGTVKEAEKRILAAAKTKTYHSISGTPEYGKCVRELMAGEGHELVSGGRACTAHTPG
                     GTAALRIGADFLKLFAQDATVWVSSPTWANHKGIFTAAGFRIKEYPYYDAVSKGLDFE
                     AMKKALRKVPAEDIVLLHACCHNPTGVDLTPEQWKEVSVLARDAGWLPFVDFAYQGFG
                     GGLEGDRAAILALAGEIPEMLVASSFSKNFGLYRDRAGALTVMASTPERAAAAFSHVQ
                     TRIRVSYSNPPAHGGLIVQTILSDASLRKEWLNELSAMRNRIASLRAHLVEGLRAMKV
                     PGDYSFIQKQHGMFSFSGLNDQQVAYLRDQKAIYMVGGGRINVAGLNDSNMKYVCESI
                     AEAVAQKK"
     gene            7658..7733
                     /locus_tag="PKK36_08665"
     tRNA            7658..7733
                     /locus_tag="PKK36_08665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7691..7693,aa:Lys,seq:ctt)
     gene            8155..8736
                     /locus_tag="PKK36_08670"
     CDS             8155..8736
                     /locus_tag="PKK36_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94665.1"
                     /translation="MAEVNFNCPRCQQSIEAPEDMVGESIKCPACGQLMTIPKPPTET
                     GSPQENTEAPKSRIERAKIKIKSLFKIRPLLWIIPALLLGVIVATVAVILLMNPLYRM
                     PEYWELRKQVGLEVSIADIRYNDYLTECKISYRDRSIHQSNIETTIRGIDDFGILEGN
                     ICGYSFLIHTRGSRPKTVKSKKAEPRPKGGSIF"
     gene            9093..>9293
                     /locus_tag="PKK36_08675"
     CDS             9093..>9293
                     /locus_tag="PKK36_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94666.1"
                     /translation="MRTVFSGHNYANGKKHGGSKILLEKICGFIGVFANVVNNDAYVY
                     YFPAASVFAGVSAVQSFSHLFRG"
BASE COUNT         2051 a         2586 c         2630 g         2026 t
ORIGIN      
        1 atcctttttc actgcggaaa gctcgatctc tttccacgat aattgacgaa gaaccaaaat
       61 aacgttaaaa atgccaaaaa cggcaaaaaa tcacgaaaaa acgccaaaaa tatgcatttt
      121 attacgaaaa atggccgaaa aatgcgcgat tttatgaaat ttgagtgaag gagggcggag
      181 atgctttcaa aacccgtaat tattttcgga accggcagaa gcgggaccac ggtgctgcac
      241 agagttctgt caagtcatcc cgagttcgcc tggatgtccg gcctgtgcga caggcatccc
      301 ggcgcgccgc agtacaaccg gtcgctgatg catgcgctgg attttccagt gcttggagat
      361 tttctgaaga ggaagtttcc cgcggatgag gggtatcggt tctgggaccg ctgttatcgc
      421 ggcttcagca ccccctgtcg ggacctgctg gcatcggatg tgaccgtccg ggcgaaagaa
      481 gacattctga agacgatgtc aaaactgatc acaaaccgcc gcgcgcgcct tctgctgaag
      541 gtcacgggct ggcctcgcac cggttttttg tccgaggtgt ttgaaggggc gaagttcatt
      601 catgtggtgc gcgacgggcg ggccgtggtg aattcgctgc tcaatgtgga cttctggcga
      661 gggtggggcg gtcccgagaa atggagatgt ggccctcttc cgccggccta tgccgaagaa
      721 tggcgccggc acagccagtc gttcgttgtc ctggccgcca tacagtggaa aatcctgatg
      781 gatgcggccg agaaggctaa acagctcctc gattccaccc gaatcatgga aatcaaatac
      841 gaagatatct gtcgcgagcc cgagaactcg ctgaaggcga ttgcgggttt ttgcgaagtg
      901 cagtggtcgg atgaatatgc ggcccgggtg cgcagattca gattcaccag cgcaaacgac
      961 aaatacagga acgagcttac gctccagcag caggcggaat tggaggatgt tttgagaccc
     1021 tgtctggccc gctgcggcta ctcatgatgt tcccgtgaat ccgggcaggc gggacggact
     1081 ttcagcacgc gcgctttggt gatgtgaaca gtgccggcct ttgcgccgcg cggcttggaa
     1141 acgcgcccgg cccgggtgca ggacactgcg gtgatcccgc cgttccgcgc cttgctgtgc
     1201 catgccgctg tcgcggccgc gcattcaaga agctcccggg aaggttcaag atgctccgga
     1261 tgccggagga tcacatggct gcccggcatg cccttcacat gaaaccacca gtccgcggga
     1321 tgggccccga tgagactcag cgcatcgttg tcgcggtcgg acttcccggc cagaatctcc
     1381 catccgtccg gaagcgcgta acggttcacc cgcaacggat tactttccgg cgtcttcatt
     1441 cgcgcgctgt tttcgggcgg tcatgatgcg ctcgttcatc cggcagaagc gcagtgtgcc
     1501 gatgagcatg acaatcacac cggccggcac gcaggtcagg ccgacaatct gaaaccacct
     1561 ggcggttgag aagtgaaaaa gggtgactcc cgcgaggatg agcgccaccc cggagcgcag
     1621 ataggccagc agcgtgcgct cattggccag cagcgtccgg tcaatggcca gctcatctct
     1681 gagagtcagg ttttcagatg tgaatcgctg atatgcagtc atatcatttt tctttgttca
     1741 tgacgcgggc tttcagggcg actcatgcca tatgccggtc aagctcttca cgcacggcgc
     1801 gccccagcac cggacggctg aacggtttct ggacgaatgc cgagcagccc attttgcgcg
     1861 cctcttcgat ggcgggctga ttggctgacc cgctgacaaa tatgcagggg agatcgggaa
     1921 ctttttcgag gatgcgctca aacaaatgca gtccgtttcc gccgccgccc atgttcatgt
     1981 ccaggagcgc cagttgaacc gtgttctcat ccagaatgtt catcgcctga tattcattgg
     2041 cggcggtcag cacgtgatag ccgagggagc tgagcagcgc ccgtgccaga tccagctgag
     2101 gctgaagatc atccacaatc agaacggttt cctcaccctc ataagaagga agctccgcgg
     2161 gcggggcggc ctgtgctgtt tccgtcgcgg ccgcaaaata gagaaggaat tccgtgccga
     2221 tgccgacttc cgaacgtatg tcgatatatc cctgatgatc cttgagaatg ccgtagataa
     2281 tggccaggcc cagccccgtt ccgctgctgc ccatctgctt gcgcgagaaa aacggctcaa
     2341 acagcctctt catgtctgcc ggttcaatcc cgtgcccctt gtccgccaca ctcagcacga
     2401 cgtaatgtcc ggcgggaatc ttctcatatc ccatcagagg atgttcaagg ttcacggctt
     2461 tcgtgctgat cagcacaggc ttcggcttgc cggcatcaat ggcgttgatg accaggttca
     2521 tgagcacggc ctgcagatgc ggcgccgagc ccatgatgtg cggcagattc tctgccagct
     2581 ggcattccag cttctgttcc ggatgccggg caaggagcat gttcaaccca agcgactttg
     2641 tgtattcctt tacgaccacg ttgaggtcca ccgaaacagc gtggaaattc ccgcggcgcg
     2701 ccagcgtcag gaggtccttg atcatttccg cggcgtgctc cgcggccttg cggatttcca
     2761 gaatgtcgtc gcgcaccgga ctctccgggg gaagctggtc aagaatcaaa tccggatagg
     2821 cgaccagtgg gccgataatg ttgttcaagt catgcgccac tttgcccgcc attttaccga
     2881 gcgtctccat ttgctcggcg ttctgcagtc gcttgcgcaa gtcgctttcc acctgttggg
     2941 ttcgccgccg ccgctcgatc ttgtccagtt cgcgccggat ggccggaccc agccgcttca
     3001 ggttgtcctt catgacatag tcactggcgc cgctgcgaat ggcatcagcc gcggtttctt
     3061 cgcccacggt gccggataca acaataaagg gagtctccgg agcggccttc aggacaatgt
     3121 cgagggcttt gagggcattg aaccccggca tgacgtagtc ggaaataata atgtccggct
     3181 tgaactggcg gagttccttg agcatgtctt tctcggtgtc cgcgcgatgc atgacgaacg
     3241 cgatgccttc ccgggaaagc acccgctgca cgagttcgca gtcctccggc cggtcctcca
     3301 gtatgagtat cttgtattgc gtttcagccg ccatgtgcgt cactgcccgt taaaagcccc
     3361 ttccgggggc ggagtgttca gaatcagcca gtacagttca aggctggaaa tcgcatttat
     3421 gaactgttca aactcaatgg gcttgaccag atagctgttg gcgccgcagt tgtacgtgtt
     3481 gatgacgtca cgttcctcct cggaggaggt caaaatgacc accggaatgg cttttgtgcg
     3541 tgcatcgccc ttgatatggc ggaggaccgt cagtccgtcc accttgggca gcttcagatc
     3601 aagcaggatg actttggggg gatggtggat gtcgcgtccg gcgtagcgct tctcgcagaa
     3661 cacatattca agggcttctt cgccgtcccg caccacatgg attctgttgg tgatgttttt
     3721 ggtttgaaga gcccggagcg tcagctcaat gtcgtctgga ttgtcctcca ccagaagaat
     3781 ttcgacagga catgcactat cactcatgaa tcagtctcca tcgggccaat ataccgtttg
     3841 tataattcac ctgtcaagat gcggctgaat ttttacccct gatcgccaag tttctgaaat
     3901 ttcgcaatga acattccgtt gcacggacca gcctccggcc agatcatgca ggcgcccgga
     3961 cagggcgccc ctgtcagcgg atgggtgaag tcggccggtt cgaaaccgga atgttcactc
     4021 aaaaaaccgg atgtcacggt ttcggtttct tcgcgggtgc aggtgcagac cgcatagacc
     4081 aggacccccc ccggccgcag atgccgggcc gcattgcgga gaatctgctt ctgaacttca
     4141 gcgctgcggc tgatcatgtc cggagcaaac ctccagcgcg catccggatt ccgcgcccag
     4201 gtgccgattc ccgaacaggg agcatccaca ataaccccgt cgaatccgcc gagaccgggc
     4261 gaatccctcc tcccgtccag cacggcagtc cgcaggcccc gcattcctgt ccgctccgcc
     4321 cgcccggcca gccggttcag gctggtttcg cgcaggtcgg atgcgagaat gtccgccagt
     4381 ccttccgtca gggccgcgag atgcagcgcc tttcctccag cgcccgcgca aacatcccac
     4441 cagcgttcac cgcgtttcgg cccggcgatc aatccgacgc actgcgaaga caaatcctgc
     4501 acctcggctt ttctgccttc cggcgtgttc aggagttcac gattgaaatt ctcgggcagc
     4561 gccgcggctt ccgacaagcg cgggtgaatc accgctccgc ctgtggagaa acggtccgcc
     4621 gtatcggcat cagcaaaccg aagccacacc gggggacggg tctgcattga accgatgaac
     4681 gatatccgcc ggccctcagg aacgggaatg tgttccagaa cacccgctgg caacagggcc
     4741 tccacggaca gttcgcggcc cagcatttgt ccggtttcac gcgccttctg ctccaaagaa
     4801 agcggagcca tcggaacagc ctgaaaaccc gccgcttcgg ccagcgctgt cagaagctcg
     4861 ctggtttccc ccgcctcaag catttgggcc attgcgcaaa cttgaaccgg agcaagaccc
     4921 ttgagccagc ccagccagcg gaaacaggag aataccatgt tcgcatataa tttgcggtcg
     4981 cgggagccgt attctctgtg tttccggaat atggcctgca gcgcggcatc cgccggcctc
     5041 ccgtccgcca cctgctcgcg cacctgctcc acaacatcca tggcgagatt cacccggcgg
     5101 ctgatgcgca gcgcgccgct gtccgaattc cggtgagtgc gattcttcat ttacggtctt
     5161 gattgcacgg catcatttaa atagcgtatt caaaaatttg gaggccggta tggcaagaga
     5221 attagcattt gttttgataa atccttatac gatcggaaaa tcaaggactg gcggagtgat
     5281 tggccgctac atccgcaccg gtctggatct ggtcggcgcg cgcatcttcg gacccagcaa
     5341 agcgcttaca gaaaaatatg cggctctgat cagggaagat ccggaagtgg atgaggatca
     5401 gcgggatatt cttgctgatt acgttctgcg gacatacatg cctgatccgc agacaggccg
     5461 ccgccatcgc gtcttcatgc tccttttcga gggcgagaac gccatcgaac gcattcgtga
     5521 agccacaggt aaggtccgct acaacatggc ttccggccgc tccgtgcgcg acatttacgg
     5581 cgactatgtc accagcccgg cgggagaagt gctctacgta gaaccggccg tgctgatcgg
     5641 tccgactctg aagtcatgcg ccgccgcgct gaaactctgg gccgaataca gcgccaagga
     5701 cgggggaatc attgctgacg cgtttgatct ggccgatgac aacatcgaaa aaacgctggt
     5761 cctgatcaaa cccgacaatt tccgttttcc gagttcgcgc gcgggcaaca ttattgacct
     5821 cttttccggg tccggtttgc gcatcgtcgg cgccaagctt catcagatga gcgttgtgga
     5881 ggccgaagaa ttttacggtc cggtgcggga attcctcagg aagaagttca agggctccgc
     5941 cggcgaccag gcctatgaag ctgtggccaa aaaattcggc attgagatcc cggaaacgat
     6001 gaaggaggcg ctgggcgaga tgttcggccc cctggtggcg gatcatcagt tctacaagat
     6061 cattgaattc atgaccggct ggtgggcgcc gtcctgcgga atcgacggca aggactgtcc
     6121 gggaaaaagc aaatgcctgg ccctcgttta tgcgggagaa aatgccatag ggaaaatccg
     6181 cgatattctc gggccgactg accccagcaa ggctcagccc ggctcggtgc gcaaggagtt
     6241 cggaagcgat gtgatggtca gcgccgccca cgcctccgat tcgcccgaaa acgccgcccg
     6301 cgaaatgaag atcatcaagg tcgagaagga caccatcaag aaactggtgc agcagtacta
     6361 tccatagaga aaaacattac aaggagtgaa tatgttcgag gaaatccagc ccgccccggc
     6421 cgatgcgata ctgggactga ctgaagccta taaaaaggat ccccgccccg tgaaagtgaa
     6481 cctgggcgtc ggggtctatc aggacgaaca ggggcacacc ccgattcttg gcacagtgaa
     6541 ggaggccgag aagcgaatcc tggcagcggc gaagaccaag acataccact cgatttccgg
     6601 cacgccggaa tatggaaaat gtgtccgcga actgatggcg ggcgagggcc acgaactggt
     6661 ttccggcggc cgcgcctgca cggcgcatac tccgggagga accgctgcac tccgcattgg
     6721 cgccgatttc ctgaaactgt ttgcgcagga tgcgacggtg tgggtaagtt ccccaacgtg
     6781 ggccaaccac aaagggattt tcacggcagc cgggttccgg atcaaagaat atccctatta
     6841 cgatgcggtg tcgaaaggtc tcgattttga ggccatgaaa aaggccctgc gcaaggtgcc
     6901 tgcggaggac atcgttctgc ttcacgcctg ctgtcacaat ccaacgggag tggatctaac
     6961 cccggaacag tggaaagaag tctccgtcct ggcaagggat gccggctggc ttccatttgt
     7021 tgactttgcc tatcagggtt tcgggggcgg acttgaaggg gaccgcgccg ccattcttgc
     7081 actggccggg gaaatcccgg aaatgctggt ggccagttcg ttctcgaaaa acttcgggct
     7141 gtaccgcgac cgtgccggcg cgctgacggt catggccagc actcctgagc gcgcggctgc
     7201 agccttcagc catgtgcaga cgcgtatccg cgtcagttat tccaacccgc cagcccatgg
     7261 cgggctcatc gtgcagacaa ttctcagcga tgcgtcgctg cgcaaagagt ggctgaacga
     7321 actgtctgca atgcgcaacc gcattgcatc gcttcgggcg cacctggtgg aggggctcag
     7381 ggccatgaag gtgcccggcg actattcctt catccagaaa cagcacggca tgttttcttt
     7441 cagcggcttg aatgatcagc aggtcgcata tctgcgcgac cagaaggcca tttacatggt
     7501 cggcggcgga cgcatcaatg tcgcgggcct taacgactcc aacatgaaat acgtgtgcga
     7561 gagcatcgcc gaagccgttg cgcaaaaaaa gtgaaaaaaa tcatttgaaa gattgccact
     7621 tgcgctatct ttctgtatca attcagttcc ctttggtgga ggcgtagctc agttggtaga
     7681 gcagctgact cttaatcagc gggtccaggg ttcgagtccc tgcgcctcta ccagtttttt
     7741 ccttaataat cacaagggga aaactgcatt tcgatcaatg tttgcaatgg tgctgtgaat
     7801 atcgctcctt gcgcttgttg cctcttatgg cgttcaatgg ctttccgtgg atatgtctca
     7861 aaatcctttt acatttgaga cagtttagga cagtccaaaa gattcagcga gaagtcagct
     7921 gctccgcatt gtctcaaatg ttgtcccaaa tgaggccggg ggcgggtggg tttgttgata
     7981 catctccggg gatgatcgtc ggttctggcc tccgtagggg cctccctcgg cgtgccgtgg
     8041 ggttaggggg tccggcctgt gggtgtgtcg cattacgaat taaatcagtt gccgtactca
     8101 agcacgggat atagcatatt gacgaatgca aaccatgcaa cgggaggaat ggacatggct
     8161 gaagtaaatt tcaattgccc gcgttgccaa cagagtattg aagcccctga agatatggtc
     8221 ggtgaaagca taaaatgtcc cgcttgcggc cagcttatga cgattccgaa acctcctacg
     8281 gaaaccggtt ctccacaaga aaacacagaa gcccccaagt cgcggataga acgagcaaaa
     8341 atcaaaatta aaagcctttt caaaataaga ccgttgcttt ggatcatccc agccttatta
     8401 ttgggggtca ttgtcgcaac cgtggcggtg attctgctga tgaatccatt gtatcgaatg
     8461 ccggaatatt gggaacttcg gaaacaagtg ggccttgaag tttctatcgc ggatatacga
     8521 tataacgatt atctaacgga atgtaaaatc tcatatagag acagatcaat acatcaatcc
     8581 aacatcgaga ccaccatccg gggcattgat gatttcggaa tactggaagg taatatttgt
     8641 ggatattctt tcctcattca tacaagggga agcagaccaa agactgttaa atccaagaaa
     8701 gctgaaccaa ggcccaaggg cggatcgatt ttctaacgtg ccacttttcg tcaagttttt
     8761 aaaaagtcgg gaacagggga tatatacata tcaagagtgc tggcagcccc ctcggggggg
     8821 agtctattga atgacaatga ttgtcctgtg tccacgatga tgaaccccgc cgcccccgac
     8881 acgctgacgc tgtgctgagg tcttgcctgt atcggttgaa ggtatgcggc ccgcctctat
     8941 ccgtttgcaa atcctcacac gttgcgcctc gatctctccg ctggtgtgat cctgttgcgg
     9001 gtacagaagc ggcaattatg gacgtgatgc actgtcaaac cgcgtagggc aggggctggt
     9061 cacaggatgg atatacgccc caccgcctca aaatgcggac ggttttcagc gggcataatt
     9121 atgcaaacgg caaaaaacac ggtggttcaa aaatactact cgaaaaaata tgcggtttca
     9181 ttggtgtttt tgccaacgtc gtgaataatg atgcttatgt ctactatttt cccgccgcgt
     9241 cagttttcgc gggggtatct gctgttcaaa gtttctctca cctgttccgg ggt
//
LOCUS       DAOQWR010000105         9055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8184_length_9055_cov_6.008667, whole genome shotgun sequence.
ACCESSION   DAOQWR010000105 DAOQWR010000000
VERSION     DAOQWR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8184_length_9055_cov_6.008667"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKK36_08680"
     CDS             complement(<1..689)
                     /locus_tag="PKK36_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR94667.1"
                     /translation="MIQHTIHFDNAREAAQLGALPAALMAQIEAVLRVRLTARDTWLT
                     IEGARTDTERAVRFYEALRKARNRDIWLGEPGIAYALRAFAEGCESDLEKLYDCRIAV
                     GRGKPPVSPRTFGQMAYLRSITERDVAFGIGPAGTGKTYLAMAMAVSALLKEEVSRIV
                     LTRPAIEAGEALGFLPGDYQQKVFPYLRPLYDALYDMVAPEDMERYVERGIIEVAPLA
                     YMRGRTLNHAFV"
     gene            complement(870..3707)
                     /locus_tag="PKK36_08685"
     CDS             complement(870..3707)
                     /locus_tag="PKK36_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR94668.1"
                     /translation="MRVKRLIWAVLLVAGLSRADVILTPSFRALPGETEPTPWLLYRN
                     VMATGVVVCGSWSDWSTNHAMILTNGIWQLDVRPLELPFGQHRYKFVVNGQWEPGADR
                     FFYASGDNLLEKPSDWIVRAAIQDRRRIEVFLKNPLASGAGLEVRLEPGPGLEEWHFA
                     SGAEGGRRQGYAVVQQLVSFYFDEKLYGLDISSTTLVTVAGNFNGWNPQGGDRWRLTD
                     PDNDGIWEGTAHIQGLRPPAGEREMAFKFVLNGKEWLPVLETAPNVLDDGRGNANLHL
                     DLGARGSSSLIITTAEDIDLSFPPVVAIDGLQPRTVRQAATPGELLDKMFSDKPLGVT
                     LDKEQGATTYRIFAPRAREVHLCLFDLPQYEIHKPAYKRLKPSERYPLWKDPADGVWE
                     ITLMGLDEGRWYSFNVDGPRGEGEGFYPEGQIGDPYARAAAHAENNTIVIDPDAPNKW
                     FKGWTDQDFVTPPLQDIVIYEAHVRDLTAHPSSGVPAPLRGLYEGIAATSGTGTGLDH
                     IRDMGFNAIELLPVCEFNNGERDYNWGYSTVFYFAPEASYAQEPVRGSQYYEFKALVN
                     ELHRQGFAVILDVVYNHVGSPNIFHMLDKKYYFRLNADYSYINFSGCGNDVRTEAPMM
                     RRLITDNIRYWMEEFHVDGFRLDLAELIDMDTMLAIRDTALSINTNAILISEPWSFRG
                     ENKQELKGTGWSAWNNDFRYAAKDFVSGRHNRDWLQKQIFGSVETWAADPLQPVNYLE
                     SHDDMALADELCTRPDRDGRNLQQADVDVNRLAATILFTSLGVPMVAEGQEFLRSKHG
                     HHNTYDKGDGVNALNWKDRDRPMAAEAMAYYRGLSRLRGSPQGAAFRVRSRPPADYFQ
                     WILPPHPQLLGYIVNAGRVHDGASFIVLLNASSSHGTFPITFPEGQWKQISDGRRLDM
                     AGLPGAPVIKAPVSTPIPVPGLHAAIFMDGF"
     gene            complement(3740..4447)
                     /locus_tag="PKK36_08690"
     CDS             complement(3740..4447)
                     /locus_tag="PKK36_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNR94669.1"
                     /translation="MTEKTAVFFDIDGTLLDTHGAGRDAFSNTLKAVFDHDDDLSYIH
                     FAGATDLDLFNEIRIRSGLKVSRMQEKEFFERLPVELRKSIREHLATLCPGVCELLKE
                     LSADERVLVGLVTGNTEECAHIKLSHFNLHGHFMLGAFGREHARRPELARRARERAKK
                     LLKRGEQLGRCFLIGDTPADIAAARAIGAVSIAVATGSYDQQSLMHAGADHVLPDLSS
                     TDRVIDILGLNRESSQT"
     gene            complement(4483..6117)
                     /gene="purH"
                     /locus_tag="PKK36_08695"
     CDS             complement(4483..6117)
                     /gene="purH"
                     /locus_tag="PKK36_08695"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNR94670.1"
                     /translation="MSATARALLSVSDKSGLVNFARDLASRGVELISTGGTAAALREA
                     GLKVTEVSDFTGFPEMLDGRVKTLHPKIHAGLLARRDKPDHMEQMTRQGFVPIDLVCV
                     NLYPFEATVAAGDVSLEAAVEQIDIGGPALLRAAAKNFGAVAAVCDPADYGRVVAEMD
                     ANGGAVPPELRLELAQKVFAHTAQYDAVVAWHLADEISSGPETRPFVMACSKGEALRY
                     GENPHQSAVLYREPVVTEACIANAELLHGKEMSFNNYVDGHAALEAVRELCGRPGAAI
                     VKHTNPCGFATGLTVASALEAAWAGDPVSAFGSVIAVTEPVTLEAAQVLKGRFVEALI
                     APAFDAEALEYLMQKSRDIRLLRLREPLHQEGPGYEVKQIGGGWLVQERDTQLAAAWN
                     VPTEIVFPESRRELALFGMMACKHVKSNAIVIVREYEKGQYALLGMGAGQPNRVDAVR
                     KLAIAKAMENIRLLYEAAGQYGLTPSQFSAGVMAESVLVSDAFFPFADNVDHAAEAGI
                     RFIVQPGGSRRDEEVIAACDRYGIAMAFTGTRHFKH"
     gene            complement(6237..6313)
                     /locus_tag="PKK36_08700"
     tRNA            complement(6237..6313)
                     /locus_tag="PKK36_08700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6277..6279),aa:Pro,seq:cgg)
     gene            complement(6477..6821)
                     /locus_tag="PKK36_08705"
     CDS             complement(6477..6821)
                     /locus_tag="PKK36_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNR94671.1"
                     /translation="MVDAAFHLHKALGPGLLESVYEVCLAHDLIKRGLKLERQVSLPV
                     FYDGLKMDAGLRLDMLVENDVIVELKAVEKLLPVHKAQVLTYLKLTGHRLALLINFNV
                     PLIRDGIQRIVL"
     gene            complement(6886..8871)
                     /gene="metG"
                     /locus_tag="PKK36_08710"
     CDS             complement(6886..8871)
                     /gene="metG"
                     /locus_tag="PKK36_08710"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNR94672.1"
                     /translation="MQTGPNLYNDASEKEDCMKRDHFYVTTPIYYVNDKPHIGHAYTT
                     ILADVLARYYRMLDVPSHFLTGTDEHGQKVWQAAQAAGITAQEQVDRTVIRFQDLWKK
                     LEITHDDFIRTTEPRHKKVVQGILQDLYDRGEIYRDEYDGWYCTPCERFFMEKDLVEG
                     CCPECKRPVEKIREANYFFRMSKYQEWLIEYINEHPQFIQPDYRRNETLGFLRKELND
                     LCISRPKSRMAWGIDLPFDDQYVTYVWFDALVNYISAVGYKSDDAQFAKWWPASYHLI
                     GKDILTTHTVYWPTMLKAMGLPMPQTIFAHGWWLSGESKMSKSLGNVVNPMDMADRCG
                     VDAFRYFLMAEMSLGQDASFTEEAFIRRYNSDLANDLGNLLSRVMKMVGSYCEGKLPP
                     PAPDALNGKDEADLWAEVQKAVAEMEKTLQGMRLDLGLAAVLAAVRATNRYLEIKQPW
                     TLAGQDDKGPLHTTLYCAAEALRVASALLSPVMPGKMQALRSTLGMDETKTDFSRLQH
                     FGLLKPGSEVKTGDPLFPRIRTEDAPAAPGKKEPEKKTMPSAPEGVALISYDDFMKVQ
                     LRTAKILNAEPVKDATKLLRLDILVGKERRQIVAGIALHYKPEELIGKTIIVVANLQP
                     AKIRGIESNGMLLAATSGAVMRLITTDGEISSGIPVK"
BASE COUNT         1855 a         2812 c         2556 g         1832 t
ORIGIN      
        1 acaaaggcat ggttcaatgt gcggccgcgc atgtaggcca gcggggccac ttcgatgatg
       61 ccgcgctcca cataccgttc catgtcctcc ggcgccacca tgtcatagag ggcatcataa
      121 agcggccgca aataaggaaa gaccttctgc tgatagtctc cgggcaagaa accgagcgcc
      181 tcgcccgcct caatggccgg gcgggtgagc acaatccggc tgacctcctc tttgagcagc
      241 gcggaaaccg ccatcgccat ggcgagatag gtctttccgg tgccggccgg accgatgccg
      301 aaggccacgt ccctctcggt gatggagcga agataggcca tctggccaaa cgtccgggga
      361 gaaaccggag gcttgccccg gcccaccgcg atgcggcagt cataaagctt ctccaggtcg
      421 ctttcgcagc cctcggcaaa ggcgcgcagg gcataggcaa ttcccggctc accgagccag
      481 atatcacggt tccgggcctt gcgcagcgcc tcgtaaaaac ggaccgcgcg ttcagtgtcg
      541 gttcttgccc cttcaatggt cagccaggtg tcccgggcgg tcagccggac gcgcaggacc
      601 gcctctatct gggccatgag ggcggccggg agcgctccga gctgggcggc ttcccgggca
      661 ttatcaaagt gaatggtgtg ctgtatcata taaatggggg ccgacgggga cgaaacccgg
      721 gcccccgaaa cgaatcaggg gcgaaccgaa gtcccggcgc atccgcaaat cgcctgatat
      781 gatctccctg catcagtcat catgtgtaaa ataaacccca taggccgctg atcgtcaatg
      841 gcaaaccgcc tgcggcgggc ctcttcggat caaaaaccgt ccataaaaat ggccgcatgc
      901 aggcccggaa cggggatcgg ggtggaaacc ggcgccttga tgaccggcgc gccgggaagc
      961 ccggccatat ccagcctgcg cccgtcggaa atctgcttcc actgcccctc ggggaaggtg
     1021 atcgggaacg tgccgtgact ggatgaggca ttcaggagaa caatgaagga ggccccgtcg
     1081 tgaacgcggc cggcattcac gatgtatccg agcagctggg gatgaggggg aagaatccat
     1141 tgaaaataat cggccggcgg acggctgcgc accctgaagg ccgcgccctg cggggacccg
     1201 cgcaggcggc tcaggccgcg ataataagcc attgcctcgg cggccatcgg ccggtcgcgg
     1261 tccttccagt tcagggcgtt gacgccgtcc cccttgtcat aggtgttgtg gtgtccgtgc
     1321 ttgctccgca gaaactcctg tccttcggcg accatcggca ctccgagcga ggtgaaaaga
     1381 atcgtggccg ccagacggtt cacatccaca tccgcctgct ggagattgcg gccgtcgcga
     1441 tcggggcggg tgcagagctc atccgccagc gccatgtcgt cgtggctttc cagatagttc
     1501 accggctgca gcgggtcagc cgcccaggtt tccaccgagc cgaaaatctg cttctgaagc
     1561 cagtcccggt tgtgccggcc cgacacgaag tccttggccg cataacggaa atcattgttc
     1621 catgccgacc agcccgtgcc cttcagctcc tgcttgttct cgccgcggaa actccacggc
     1681 tccgaaatca gaatggcatt ggtgttgatg gaaagcgccg tgtcgcggat ggccagcatg
     1741 gtgtccatgt caatgagctc cgcgagatcc aggcggaatc cgtccacatg aaactcctcc
     1801 atccagtagc ggatgttgtc cgtgatgagg cggcgcatca tcggcgcctc ggtgcggacg
     1861 tcgttcccgc atccgctgaa gttgatgtaa ctgtagtccg cgttcaggcg gaagtagtat
     1921 ttcttgtcga gcatgtggaa gatgttcggc gaaccgacgt ggttgtacac cacatccaga
     1981 atcaccgcga agccctgccg gtgcagttca ttcacgaggg ccttgaactc gtaatactgc
     2041 gagcccctca cgggctcctg cgcataggaa gcctcgggcg caaagtagaa caccgtggaa
     2101 tagccccagt tgtagtcgcg ctcgccgttg ttgaactcgc agaccggaag gagttcaatg
     2161 gcgttgaatc ccatgtcgcg gatgtgatcc agtcccgttc cggtgccgga ggtcgccgcg
     2221 atgccttcgt acaggcctcg cagcggggcc ggcacccctg aagacgggtg cgccgtcagg
     2281 tcgcgaacat gcgcttcata tatcacgatg tcctgaagag ggggcgtcac aaaatcctga
     2341 tcggtccagc ctttgaacca tttgttcggg gcgtcgggat cgatgacgat cgtgttgttt
     2401 tcggcatgcg cagccgcccg cgcatacgga tcgccaatct gcccctccgg atagaacccc
     2461 tccccttcac cgcgcggtcc gtccacattg aaggagtacc aacggccctc gtcgagtccc
     2521 atcagcgtga tctcccaaac cccgtcggcg ggatccttcc agagaggata gcgctcggag
     2581 ggcttcagcc gcttgtaggc cggtttgtgg atttcatact ggggcaggtc gaaaaggcac
     2641 aaatgaactt cacgggcgcg cggcgcgaag atccggtagg tggtggcccc ctgctccttg
     2701 tcgagcgtga ccccgagcgg tttgtcggag aacattttgt ccagcagctc tccgggcgtt
     2761 gcagcctgac gcaccgtccg gggctgaagg ccgtcaatgg ccaccacggg cggaaaggaa
     2821 agatcaatat cttccgccgt ggtaataatc agggatgagc tgccgcgggc acccagatca
     2881 aggtgcagat tcgcgtttcc gcgaccgtcg tccagcacat tcggggcggt ctccagcacg
     2941 gggagccact ctttcccgtt caggacaaat ttgaaggcca tctcccgctc ccccgcaggc
     3001 ggccgcagcc cctgaatatg tgcggtgccc tcccagattc cgtcgttgtc cggatcggtc
     3061 agccgccagc gatcccctcc ctgcggattc cagccgttga agtttcccgc gaccgtaacc
     3121 agtgtggtgc tggaaatgtc caggccgtac agcttctcgt cgaagtagaa ggacaccagc
     3181 tgctgcacca cggcgtagcc ctgccggcgg cccccttctg cccctgacgc aaagtgccat
     3241 tcctccagcc ccggaccggg ctccagacgg acctccagcc ccgcgcccga ggccagcgga
     3301 tttttcagaa acacctcaat gcgccgccga tcctgaatcg ccgcgcggac aatccagtcc
     3361 gaaggttttt ccagcaaatt gtcgcctgat gcatagaaaa agcggtcggc acccggctcc
     3421 cactggccgt tgacaacgaa cttgtaccga tgctggccga aaggaagttc cagaggccgg
     3481 acatccagct gccagatgcc gttggtgagg atcatcgcat gattcgtgct ccagtcgctc
     3541 cacgagccgc acacgacaac cccggtcgcc atgacattgc ggtagagcag ccagggcgtc
     3601 ggctcagttt ctccgggaag ggccctgaag gaaggggtca gaatcacatc cgctcgcgaa
     3661 agtcccgcga ccagcaaaac agcccatatc aaacgcttta cccgcattgt aaactccgtg
     3721 ccacttcaag ccgccagaat taagtttggg aactttccct gtttagcccc agaatgtcaa
     3781 tcactctgtc cgtggaggaa agatcaggca gcacgtggtc cgcacccgca tgcatcagag
     3841 actgctgatc atacgagccg gtcgccaccg cgatggatac ggccccgatt gcccgcgcgg
     3901 cggcaatgtc agccggggtg tcgcctatca gaaagcagcg gccaagctgt tcgccccttt
     3961 tcagcagttt ctttgcccgc tcacgggcgc ggcgggccag ttccggacgg cgcgcatgct
     4021 cccggccgaa tgcgcccagc atgaaatgcc cgtgcagatt gaaatggctc aactttatat
     4081 gcgcgcattc ctcggtgttg cccgtaacca gtccaacaag cacacgctca tcggcggaaa
     4141 gctccttcag cagttcgcag actccgggac acagcgtggc cagatgctcc ctgatggatt
     4201 tgcgcagttc aaccggcaga cgctcaaaaa attccttctc ctgcattctg ctgaccttca
     4261 gtccgctgcg aatgcgaatt tcattgaaca gatcaagatc cgtggccccg gcaaagtgga
     4321 tgtaactgag atcgtcatca tgatcaaaca cggccttcag tgtgttggag aaagcatccc
     4381 ggcccgcgcc gtgcgtatcg agcagtgttc cgtctatatc aaaaaataca gcggtttttt
     4441 cagtcatttg ccgtgaaacc gtagcatccc gcccgcccgg aatcaatgct tgaaatgacg
     4501 ggtgcctgtg aaggccatgg caatgccata acggtcgcag gcggcgatga cctcctcatc
     4561 cctgcgggaa ccgcccggct gcacgataaa acgaatgccg gcttcggccg cgtgatccac
     4621 attgtccgcg aacgggaaaa aagcatcgga aaccagcacg ctttccgcca tcacgccggc
     4681 ggagaactgc gatggagtca ggccgtactg cccggctgcc tcgtaaagca gccggatatt
     4741 ttccatggct ttggcaatgg ccagcttgcg cacggcatcc acgcggttgg gctgtcccgc
     4801 ccccatgcca agcagggcat actggccctt ttcgtactcg cgcacaatca caatggcgtt
     4861 ggacttcaca tgcttgcagg ccatcattcc gaagagcgcg agttcacgcc gggactctgg
     4921 gaacacgatt tccgtgggaa cattccacgc ggcggcaagc tgcgtgtcgc gctcctgaac
     4981 aagccagccg ccgccgatct gcttcacctc atagccgggt ccctcctggt gcaggggttc
     5041 gcgcaggcgc agcagccgga tgtccctgct cttctgcatc aggtattcca gcgcctcagc
     5101 gtcaaaggcc ggcgctatca gtgcctccac aaagcggccc ttcagcacct gagcggcttc
     5161 aagcgtcacc ggctccgtca cagcaatcac acttccgaag gccgagaccg gatcgcccgc
     5221 ccaggccgct tccagcgcgg aagccacggt caggcctgtg gcaaaaccgc acggattggt
     5281 atgcttgaca atcgccgcgc cgggccttcc gcaaagttcc ctgaccgctt cgagtgcggc
     5341 atgcccgtct acataattgt tgaaggacat ttccttgccg tgcagcagtt cagcatttgc
     5401 gatgcaggct tctgtcacca ccggctcgcg ataaagcacg gcggactggt gcggattctc
     5461 cccgtagcgg agggcttcgc cctttgaaca ggccatcaca aacgggcgtg tttcgggacc
     5521 cgaggatatt tcgtccgcca gatgccaggc caccaccgca tcgtactgag ctgtatgcgc
     5581 aaacaccttc tgcgccagct ccagccggag ctccggcggg accgcacctc cgttcgcatc
     5641 catctccgca acaacccgcc cgtagtccgc cggatcgcac acggccgcaa ctgctccaaa
     5701 gtttttggcc gccgcgcgca gcagcgccgg accgccaatg tcaatttgct cgacggcggc
     5761 ctcaagagaa acatcaccgg ctgcaaccgt ggcctcaaac ggataaagat tcacgcacac
     5821 caaatcaatc ggcacaaagc cctgccgagt catctgttcc atgtgatccg gcttgtccct
     5881 gcgggccagc aggcccgcat ggattttggg atgaagcgtc tttacacggc cgtcaagcat
     5941 ttcgggaaac cccgtgaaat cggaaacctc cgtcaccttg aggccggctt cgcggagcgc
     6001 ggcggccgtg ccgccggtgg aaatcagttc cactccgcgg gaggccagat cccgggcaaa
     6061 attcaccagg ccggacttat ccgacacgct taacagggct cttgctgtcg cactcatatt
     6121 cctctcctgc attcgctgat caaactagaa cattctgcac ctgtaatcca gacccgaatc
     6181 aaacgatatg aggccgtgca cacgtgaaaa cagaaaaaga aaacgagcca tggagatggt
     6241 cggggcggtg ggatttgaac ccacgacctc ttggtcccga accaagcgcg ctaccagtct
     6301 gcgctacgcc ccggaaagcg ctgaacaatc taaacgaatc gtggtgaaac gcaagcggga
     6361 atcctaacaa gttttcctat tttaccggaa tcccgcccga aatctcgctg gcggggtcat
     6421 ggcatctgca atcccttgaa atatttttca ccaccaagac tccaaggcag aaagcttcag
     6481 aggacgattc gctgaatacc gtctcgaatc aggggaacat tgaagttgat cagcaaagcc
     6541 aagcggtgtc ccgtaagctt aagatatgtc aaaacctgtg ccttgtggac tggcagaagt
     6601 ttttcaacgg ctttgagttc aacaataacg tcgttttcaa caagcatgtc cagacgcagg
     6661 ccggcatcca ttttcagtcc gtcatagaaa accggaagcg aaacctgccg ctcgagcttc
     6721 aatccccgct taatcaagtc atgggccaga catacttcat aaacgctttc cagaagtccg
     6781 ggcccaaggg ctttatgcag atgaaacgcc gcatccacta tattctgtgc attgcaatct
     6841 gtctcatgac ttacggcctc tcttggtgcc ttggtggttc atatcctatt ttaccggaat
     6901 gccgctcgaa atctcgccgt cggtggtgat gagacgcata accgcgccgc tggtcgcggc
     6961 cagaagcatg ccgttggatt ctatgccccg gatttttgcg ggctggaggt tggccaccac
     7021 aataatggtt ttcccgatca gttcctcggg cttgtagtgg agcgcgatgc ccgccacgat
     7081 ctggcggcgc tccttgccga ccagaatgtc cagccgcagc agcttggtgg catccttgac
     7141 cggttctgca ttcaggattt ttgccgtgcg cagctgcacc ttcatgaaat cgtcatagct
     7201 gatgagcgcc acgccctccg gcgcggacgg catggttttt ttctcgggtt cctttttccc
     7261 cggggcggca ggtgcatcct ccgtcctgat tcgcgggaac agcggatcgc ccgtcttcac
     7321 ttccgaaccg ggctttagca gcccgaaatg ctgaagcctc gagaaatcgg ttttggtttc
     7381 atccataccc agcgtcgagc gcagggcctg cattttgccg ggcatgacgg gcgacagcag
     7441 agccgacgcc acgcgcagcg cctccgcggc gcagtagagt gttgtgtgca gcgggccctt
     7501 gtcgtcctgc ccggccagcg tccacggctg cttgatctcc agatagcggt tcgtggcgcg
     7561 caccgcggcc agcacggcgg ccaggcccag gtcgagccgc atcccctgca gggttttctc
     7621 catttcagcc acggccttct ggacttcggc ccataaatcg gcctcatctt tgccattcag
     7681 cgcatccggc gcgggcggcg gcagtttgcc ttcgcagtag cttcccacca tcttcattac
     7741 gcggctcagc agattgccca gatcattcgc cagatcagag ttgtaccggc ggataaaagc
     7801 ctcctccgtg aagctggcat cctgccccag actcatctcg gccatcagga agtagcggaa
     7861 tgcatccacg ccgcagcggt cggccatgtc catcggattc acaacattgc cgagcgactt
     7921 gctcattttg ctttcgccgg acagccacca gccgtgagca aagatggtct gcggcatcgg
     7981 cagacccatt gccttgagca tggtgggcca gtacaccgtg tgcgtggtca gtatgtcctt
     8041 gccgatcagg tggtacgacg ccggccacca cttcgcgaac tgcgcgtcat cggatttgta
     8101 gccgacggcg ctgatgtagt tcaccagcgc atcaaaccag acataggtca catactggtc
     8161 gtcaaacggc agatcaatcc cccacgccat gcggcttttg gggcgcgaga tgcagagatc
     8221 attgagttct ttgcgcagga agccgagtgt ttcattcctg cggtaatccg gctggatgaa
     8281 ctgcgggtgc tcgttgatat attcgatgag ccattcctga tatttgctca tccggaagaa
     8341 ataattggcc tcccgaattt tttccaccgg acgtttgcac tccgggcagc agccttcgac
     8401 aaggtccttt tccatgaaaa aacgctcgca gggcgtgcag taccagccgt cgtactcgtc
     8461 gcggtaaatt tcaccgcggt cgtacaggtc ctgcaggatt ccctgcacca cttttttgtg
     8521 ccggggctct gtggtgcgga tgaaatcatc atgcgttatc tccagcttct tccagagatc
     8581 ctggaagcgg atcaccgtgc ggtccacctg ctcctgggcc gtgatgcccg ccgcctgggc
     8641 cgcctgccag accttctgcc cgtgctcgtc cgtaccggtc aggaaatgcg aggggacatc
     8701 cagcatgcgg taatagcgcg ccagcacatc cgcaagaatc gtcgtgtagg catgcccgat
     8761 gtgcggcttg tcgttgacgt aataaatcgg cgttgttaca taaaaatgat ctcgcttcat
     8821 acagtcctct ttttcagaag cgtcattata gaggttgggc cccgtttgca aagcattcaa
     8881 tgatttttgt cacgatttcc agtgcctggg aagttcctgc gggaaaagtt ccaatcattg
     8941 gaagtttcgc cgccaaaagt tccaatgcct ggaagttttt gtttggaaag ttccaatggc
     9001 tggaactttt cggaccgcgc cggagcgcta aaaaaagcgg ccggagagcg attcc
//
LOCUS       DAOQWR010000106         9022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8209_length_9022_cov_4.940448, whole genome shotgun sequence.
ACCESSION   DAOQWR010000106 DAOQWR010000000
VERSION     DAOQWR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_9022_cov_4.940448"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="tsf"
                     /locus_tag="PKK36_08715"
     CDS             complement(<1..348)
                     /gene="tsf"
                     /locus_tag="PKK36_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNR94673.1"
                     /translation="MAEITAQLVKELREATNVGMMDCKRALAETNGDKDAAIKLLRER
                     GIAVAGKRAARAAKEGIVASALINDGKSGVMIEVNCETDFVSRNENFQAFVKDMLEKA
                     KTVKGDLAEAVKDD"
     gene            complement(374..1369)
                     /gene="rpsB"
                     /locus_tag="PKK36_08720"
     CDS             complement(374..1369)
                     /gene="rpsB"
                     /locus_tag="PKK36_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNR94674.1"
                     /translation="MVTKGEIQTDATIQDLLDAGLHFGHQTKRWNPKMKRYVFGERNG
                     IYIIDLEKTMQGLKAAQQFLYDTVIRGKSVLFVGTKKQAAETLEETAKRLNQFYVTHR
                     WLGGTLTNNQTIRQSVSRMRKLDAMEKDGSLETHPKKEVAQLRRENTKLHRNLDGLAS
                     MSDLPGVLVVFDITREAIAVAEAKTLGIPVIAMVDTNCDPTPIDYPIPGNDDAIRAVR
                     LITKILGDTILQASNEYAQMAAEEARKRAAAEAERKAKEEAAARERKAREDAARIEKA
                     KALAKAKKETEAKKAKESAEGKPAEEPAAAAEAQAEAPAAEAAPAAEAAPETPAE"
     gene            1619..2011
                     /locus_tag="PKK36_08725"
     CDS             1619..2011
                     /locus_tag="PKK36_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HNR94675.1"
                     /translation="MEELIGLLVKQVNVNESQAKGGAGLLFKLAKDKLASGDFSKIAS
                     AVGGVDELIKSAPKAGGASKLLGSLASAAGGDKLGGLGQLASLAGGLKSLNLSGDTLS
                     RFIPVILGFVQGRGGSAVKDILASVLKV"
     gene            2008..2790
                     /gene="xth"
                     /locus_tag="PKK36_08730"
     CDS             2008..2790
                     /gene="xth"
                     /locus_tag="PKK36_08730"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNR94676.1"
                     /translation="MTVFRAATFNANSIRSRLEIVLAWLDKWKPDILCIQETKCQDHD
                     FPREAVESAGWHVAFRGEKSYNGVAIISRRPLEAVRFGLDDGGPADETRLICAQAGGI
                     TVVNTYVPQGREIDHAMYAYKLEWLKRLRAYFDRHFKTTEKLIWTGDLNVARTPLDVY
                     DAASKKDHVCYHEAVRAVFEECLAWGFTDVFRKFHEGEEKYSFFDYRAPSSIAQNRGW
                     RIDYILASAPLAKKAKDSLIDMEPRLKPKPSDHTFLVADFEG"
     gene            2865..3170
                     /locus_tag="PKK36_08735"
     CDS             2865..3170
                     /locus_tag="PKK36_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94677.1"
                     /translation="MKKVVTLLNSMVADQVIKGYALFDAVAQMRYTEAVATLDADVLV
                     SLPSDGGLDVLSPVYRYCESRGYRPEGEAIRVGDWPVQFIPVYNSLSDRSAQRGRTR"
     gene            3371..3574
                     /locus_tag="PKK36_08740"
     CDS             3371..3574
                     /locus_tag="PKK36_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94678.1"
                     /translation="MTDVNKMFKKQAAWQQARTELPWAEKLRQSLVLREARRSLKKTV
                     REPEEKYPAGHDEDGHVAITPEE"
     gene            3579..4244
                     /locus_tag="PKK36_08745"
     CDS             3579..4244
                     /locus_tag="PKK36_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNR94679.1"
                     /translation="MKKQKVILHNSVSLNGSLLGFDVDMEKHYQIAGSYQADVHLIGS
                     NTVEAGIKMFCPEIPEEEKSDFKKPERSRSLPLWVIPDTTGKLQGLHHVYRRFELCRD
                     IVILVSRKTPQKYLDYLKERNYDCHVVGEDHVDCRRALELLAKQYDAKTVLTDTGRIL
                     NCILINEGLVDEISLLVHPVIVGTNAYTLFDGVKGDIKLELAGTKEMGGGKVWLSYRV
                     QQA"
     gene            4625..5098
                     /locus_tag="PKK36_08750"
     CDS             4625..5098
                     /locus_tag="PKK36_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94680.1"
                     /translation="MMNTNEHCCPKFDPAPWDEKEIQWQERRFVKDRVKSFLHIPLNF
                     GAVMKRSMARIEAAGATSDTNMVLSDENSLWGADVYIEVAKDVPGANMASISGTFLCK
                     VFEGPYQNMHKWIAEMKDFVQGRGKAIRKFYFYYTTCPKCAKKYGKNYVVILAQI"
     gene            complement(5115..6665)
                     /locus_tag="PKK36_08755"
     CDS             complement(5115..6665)
                     /locus_tag="PKK36_08755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR94681.1"
                     /translation="MSAESDHVEIPGFEMLSVAGAGGMATVWHARQLSLDREVAIKVL
                     FPSLVRDPESVRLFRQEARAAARLRHPGIVQVYDAGEHEGMVYLVMEFVSEGTVGDLL
                     ERKGCLPESDGLAIIEALAGALQYAWDAAHIIHCDIKPDNVMIDGNGEIRIADLGLAR
                     VVESMAQDNRTQNIMGTPNYTSPEQARGDETLDCRSDIYSAGAMLYHIVTGKIPFPDP
                     DPGRILEFQNVGYLTDPLDIKPDLFPGTAWIIEKMMAKDPAHRYQSWNELIEDLRLVD
                     KQRMPASAMPEEGQSTVLRGKHRREPPAAVKGMPPRKKIVIDAQTRAELQQRKKKNSG
                     FMRMLAWLTFWAALGTGGYKGWEFYMAENPKPVPQAAAKEIEEALPPRAYRTQEDGAP
                     IPAAAGPSFRRVTAEPENSSSWDSPVFRQGAEAFNSALEDYKKYQATRTNPGMLKDIE
                     SRCRLAIASFEAVRDEAPPERDMDFYIKQAYQLISDCRQSTLMPAPEQKDSRFQPAPE
                     LPPPSWAR"
     gene            6818..7711
                     /locus_tag="PKK36_08760"
     CDS             6818..7711
                     /locus_tag="PKK36_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNR94682.1"
                     /translation="MKHFSVILKSLAVLMLLGLASCTSIDAVTGERVYNLYSMQSEIN
                     LGVQTIQANTAEMQKQGCRINDDPKQMARLGEITKRITTVADMPQLPYEVTLYHTNIV
                     NAAAAPGGQMFVFEGLYDKEIGLCRDDDELAAVMAHEIAHVNCRHSTERLSKLMTAAA
                     VVEVGAIALENEGESDWATGLRAAFAVGTVFYVPMHSKKDEFEADRVGLFYMAKAGYD
                     PRAAPRIWRRVAEEDPDEKDKASIFATHPSAGDRCDALEAMVPYAMQEYVRATGSYPK
                     DYKPENDGLPEFDWRRPGARK"
     gene            complement(7805..8710)
                     /locus_tag="PKK36_08765"
     CDS             complement(7805..8710)
                     /locus_tag="PKK36_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94683.1"
                     /translation="MNITRFMIFAASLLTAASLTASELQIKGGTLWVGAELQSDEVMV
                     DGLGTLGGTGTVAAGLVGVSGTVSPGSSSGAQGALSFSSNVVFQPGSFYDCDVASSTS
                     VDRLNIRGAAGGQGTVRVSNPSGAIPVDQVIIDGGDSSGYGSFQTIGAGLMRWGLAEN
                     PPTDLSLTRLDGDTDGNGLPDWWELAWFGQRTGTDPDGHGDADGVPNEEEYLAGTDPT
                     DETSVLQLTWIERDASGGISMGWSSTNSPWLNVPSSPYRVEATTNIIEDGFIALSGNL
                     SPTPPENVYTNAVSADYRLYRVVIP"
     gene            complement(8723..>9022)
                     /locus_tag="PKK36_08770"
     CDS             complement(8723..>9022)
                     /locus_tag="PKK36_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94684.1"
                     /translation="DGKAVVELPAYYSALNKAGSEIYSLTPIGEAFVWVAEEVADNRF
                     TIAGKQDIKVSWTVKALRNDPACRADIEQRPVEQNLSEIAPGQMQAENTGMNTMP"
BASE COUNT         1841 a         2605 c         2676 g         1900 t
ORIGIN      
        1 atcgtccttg acggcctcgg ccaggtcgcc cttgacggtc ttggccttct ccagcatgtc
       61 cttcacaaac gcctggaagt tttcgttgcg cgaaacgaag tcggtttcgc agttgacctc
      121 gatcatcacg ccggacttgc cgtcgttgat gagggcggag gccacaatgc cttccttggc
      181 ggcgcgggcg gcgcgcttgc cggccacggc aatgccgcgc tcgcgcagca gtttgatggc
      241 cgcatccttg tcgccgttgg tctcggcgag ggcgcgcttg cagtccatca tgccgacgtt
      301 ggtggcttcg cgcagttctt taacgagttg tgcagtgatt tctgccattt ttgctcagtc
      361 ctttcgggag taattattca gcaggagttt caggcgcagc ctcggcggcc ggtgcagcct
      421 cagcggccgg cgcttcggcc tgggcctcgg ccgccgccgc aggttcctct gccggcttgc
      481 cttcggcgct ttccttggcc ttcttcgcct ctgtttcctt cttggccttg gccagagcct
      541 tggctttctc gatgcgggcg gcgtcttcac gcgccttgcg ctcgcgggcc gcggcttctt
      601 ccttcgcctt gcgctcggct tcggcggcgg cgcgcttgcg cgcttcctcg gcggccatct
      661 gcgcgtattc gttcgaggcc tgcaggatgg tgtcgcccag gatctttgtg atcaggcgga
      721 cggcgcggat ggcgtcgtcg ttgccgggga tcggatagtc aatcggcgtc ggatcgcagt
      781 tggtgtccac catggcgatg accgggatgc ccagcgtttt ggcttccgcc accgcgatgg
      841 cttcgcgggt gatgtcgaaa acgacaagca caccgggcag gtcagacatg ctggccagtc
      901 cgtccaggtt ccggtgcagc ttcgtgtttt cgcggcggag ctgcgcgact tccttcttgg
      961 gatgggtttc caggctgccg tccttctcca tggcgtccag cttgcgcatg cggctgacgc
     1021 tctggcggat cgtctggttg ttggtcagtg tgccgcccag ccagcggtgg gtgacataaa
     1081 actggttcag gcgcttggcg gtttcctcca gcgtttccgc cgcctgcttc ttggtgccca
     1141 cgaagagcac gctttttccc cggatgacgg tgtcatagag gaactgctgg gccgccttga
     1201 ggccctgcat tgttttttcc aggtctatga tgtaaatccc gttgcgctcg ccgaagacat
     1261 agcgcttcat cttggggttc catcgtttgg tctgatgacc gaagtgcagg cctgcatcga
     1321 gcagatcctg tattgtggcg tctgtctgta tttcgccctt agttaccatt ttcaacctca
     1381 ctgtctggtt ctgtgccttt ggcacggatc aatccgcttt ggctctccga ggcgggagaa
     1441 cactcgcttc cgctccgcag cgacgtgcgc ccggagcccg tttaaggcgc cgaatatagc
     1501 atgccgcttc cctgccgcaa gggaaaaacc ggattatttt tccagacgcg gacgctatag
     1561 tgttgatgtc ttcccggcgg cctgcaacaa tgaactctca acagaaaggg gaaatgtcat
     1621 ggaagaactc atcgggctgc tggtcaagca ggtcaatgtc aatgaaagcc aggccaaggg
     1681 cggggcgggg ctgctgttca agctcgcgaa ggacaagctc gcctcgggcg acttctccaa
     1741 gattgcatcc gcggtcgggg gggtggatga actgatcaag tcggcgccga aggcgggcgg
     1801 cgcctcaaaa ctgctgggca gcctggcctc ggcggcgggc ggagacaaac tgggcggcct
     1861 cggacagctc gcttcgctgg ccggcggact caagagcctg aacctcagcg gcgacaccct
     1921 ttccagattc attcccgtca tcctcggatt tgtccagggc aggggcggga gcgccgtgaa
     1981 ggatattctg gcatcggtgc tcaaggtgtg acggtgttca gggccgccac cttcaacgcc
     2041 aattccatcc gaagccggct cgaaattgtt ctggcctggc tggacaaatg gaaacccgac
     2101 atcctgtgca tccaggaaac caaatgccag gaccatgact tcccgcgtga agccgtcgaa
     2161 tccgccggat ggcatgtcgc tttcaggggc gagaagagct acaacggcgt ggcgatcatt
     2221 tcccggcggc cgctggaggc ggtccgcttc ggcctcgatg acggcggccc cgccgacgaa
     2281 acccggctga tctgcgcgca agcgggcgga atcaccgtgg tgaacaccta tgtcccgcag
     2341 ggccgtgaga tagaccacgc gatgtacgcc tacaagctgg agtggctgaa aaggcttcgg
     2401 gcctatttcg accggcactt caaaacgacg gaaaaactga tctggaccgg cgatctgaac
     2461 gtggcccgca ccccgctgga tgtgtacgat gccgcttcaa agaaagacca tgtctgctat
     2521 cacgaggcgg tccgcgcggt tttcgaagaa tgccttgcat ggggtttcac ggatgtgttc
     2581 cgcaaattcc acgagggcga agagaaatat tcattcttcg actaccgcgc accgagctcc
     2641 atcgcgcaga accggggctg gagaattgac tacatcctcg ccagcgcccc gctggcgaag
     2701 aaggcaaagg attccctcat agacatggaa ccccggctga aacccaaacc ttctgaccac
     2761 accttccttg tggccgattt tgaaggttga acaacctcaa atgcatgcag gcctcccgcg
     2821 atgttttctt gctgattaca gcaagtcatg ctaagggctg aagcatgaaa aaagttgtga
     2881 cgctgctcaa cagcatggtc gcggaccaag tgattaaagg ctatgccctg tttgatgccg
     2941 tcgctcaaat gcgttacacg gaagccgtgg ccactcttga cgctgatgtg ctggtaagcc
     3001 ttccgtccga tggcggtctt gatgttcttt cacccgtcta cagatactgt gaatccagag
     3061 gttacagacc ggaaggagag gccattcggg ttggcgactg gcctgttcag ttcatacccg
     3121 tatacaattc gctttctgat agaagcgctc agagaggccg aaccagatga atttgagggc
     3181 gttcctgtca gagttgtgcg ggcgaattac ctggctgtga tcgccttgag tgtcggccgt
     3241 cacaaggatt atcttcggat actggcctta ttggattccg gtttcgctga taggaaaacg
     3301 atctctgaaa ttgcgtcgcg gcatggcctt gcaggcgcat gggaacaatt caaagagaag
     3361 tttgtgaaca atgactgacg tgaacaaaat gttcaaaaag caggccgcct ggcagcaagc
     3421 cagaaccgaa ctgccctggg cggaaaagct ccggcagtcc ctggtgcttc gtgaagcccg
     3481 gcgttccctg aaaaaaacag tccgtgaacc agaagaaaaa tatccggccg gtcatgacga
     3541 ggacggtcat gtggccataa caccggaaga atgacctcat gaaaaagcaa aaggtgattc
     3601 tgcacaattc ggtcagcctg aacggttcac tcctgggatt tgatgtggac atggagaagc
     3661 attaccagat cgcggggagc tatcaggcgg acgttcacct tatcggctct aacaccgtgg
     3721 aagcgggaat aaaaatgttc tgtccggaga ttcccgaaga ggaaaaaagc gatttcaaaa
     3781 agccggagcg aagcagaagc ctgccgctat gggtcattcc cgacaccacg ggaaaactgc
     3841 agggcctgca ccatgtctac cgaagattcg aactgtgccg ggacatcgtg attctggttt
     3901 cccgaaaaac gccgcagaaa tatctcgatt atctgaaaga gcggaattac gactgtcacg
     3961 tcgtcggcga agatcatgtg gattgccgcc gcgccctgga actgctggca aagcagtatg
     4021 atgcaaaaac cgttctgacc gataccggaa gaatcctgaa ctgcattttg atcaatgagg
     4081 ggcttgtgga tgaaatcagt ctgctggtgc atcccgtcat cgtgggcaca aacgcgtaca
     4141 cgctgttcga cggggtgaaa ggggacatca aactggaact ggccggcacg aaggaaatgg
     4201 gcggcggcaa agtctggctg agctacagag tacagcaggc gtaaagttcg gattccaggc
     4261 ttaaaaaaac ggccaaaaag tgccaaaaaa cgctcaaaaa tgccaaaaac gggctcaaaa
     4321 tggccaaaaa acgctcaaaa acagttaaaa tggcccaaaa cggccattta tcttcagaaa
     4381 ttgccccaaa atgcttaaaa acgtccaaaa tcgtgtattt cattaaatag tactaaatta
     4441 gtactattta catgatcgtc cgcccgcagg aaggtttttc atgggacggc ggttcctgtg
     4501 ccgtccacct ccgccctttc tcccccgcga cgcaaggtcg cgggggccga ttcgatgtct
     4561 cgccaaatcc cggttgacac ccgccccgaa accgtcagta tcaccacaag ggaaaggagc
     4621 atgaatgatg aatacaaatg aacattgctg tccgaagttt gatccggcgc catgggacga
     4681 gaaggaaatc cagtggcagg aaaggcggtt cgtgaaggac cgggtgaaaa gtttcctgca
     4741 catccctctg aatttcggag ccgtgatgaa acgcagcatg gcccgcattg aggcggcggg
     4801 cgcgacttca gatacgaaca tggttctgtc ggatgagaac tctctttggg gcgccgatgt
     4861 gtatatcgag gtggccaaag acgttcccgg tgcaaacatg gccagcatct ccggaacatt
     4921 tctctgcaaa gtttttgaag ggccttatca gaacatgcat aaatggatcg ccgaaatgaa
     4981 agatttcgtc cagggcaggg gaaaggccat ccgaaagttc tatttctatt acacaacctg
     5041 ccccaaatgc gcgaagaagt acggcaaaaa ctatgtagtg atcctggcgc agatatagag
     5101 cgcgcggccg gcggtcacct tgcccagctt gggggcggga gttcgggggc gggctggaag
     5161 cggctgtctt tttgttccgg ggccggcatc agggtggact ggcggcagtc ggagatgagc
     5221 tggtaggcct gcttgatata gaagtccatg tcgcgctccg gcggcgcttc gtcgcggacg
     5281 gcctcgaatg acgcgatggc cagccggcag cggctttcaa tgtccttcag cattccggga
     5341 ttggtccttg tggcctgata ctttttatag tcttcgagcg cgctgttgaa ggcctccgcc
     5401 ccctgccgga agacggggga atcccatgac gatgagtttt ccggttctgc cgtgacgcgc
     5461 cggaaagagg gacctgccgc ggccgggatc ggagctccgt cctcttgcgt ccgataggcg
     5521 cggggcggaa gcgcctcttc gatttccttc gccgccgcct gcggaacggg cttcggattc
     5581 tcggccatgt agaactccca gcccttgtat ccgccggttc ccagggcggc ccagaaggtc
     5641 agccaggcca gcatccgcat gaatccggag tttttctttt tgcgctgctg aagttcggcc
     5701 cgcgtctggg catcaatcac gatctttttc cggggcggca tgccctttac agcggccggc
     5761 ggctcgcgcc ggtgcttgcc gcgcagcacg gtgctttgtc cttcctccgg catcgcactg
     5821 gccggcatcc gctgtttgtc aacgagcctc aggtcctcga tcaactcgtt ccagctctgg
     5881 tagcggtggg ccggatcctt ggccatcatt ttctcgatga tccacgccgt gcccgggaac
     5941 aggtccggtt tgatgtccag cggatcggtc agatagccca cattctggaa ttccaggatg
     6001 cggcccggat cggggtcggg gaagggaatt ttgccggtga cgatatgata gagcatcgcg
     6061 ccggcggaat agatgtcgct ccggcagtcc agtgtttcgt ccccgcgcgc ctgctcgggc
     6121 gaggtgtagt tcggcgtgcc catgatgttc tgcgtgcggt tatcctgcgc catggattcc
     6181 acgacgcgcg cgaggcccag atccgcgatc cggatttctc cgttcccgtc tatcatgacg
     6241 ttgtcgggct tgatgtcgca gtggatgatg tgcgccgcgt cccatgcata ctggagcgcc
     6301 ccggccagcg cctcgatgat ggccaggccg tcggactccg gcaggcatcc cttgcgctcc
     6361 agcaggtcgc cgacggtgcc ttcggagaca aactccatga ccagataaac catgccctcg
     6421 tgctcgcccg cgtcatagac ctggacaatg ccgggatgcc ggagtctggc ggccgcccgc
     6481 gcctcctgcc ggaacaggcg gacggactcc gggtcgcgca cgagactggg gaaaagcacc
     6541 ttgatggcga cctcgcggtc gagcgacagc tgccgcgcat gccagaccgt ggccatgccg
     6601 ccggcgccgg ccacggacag catctcaaac cccggtattt ccacatggtc cgattctgcg
     6661 ctcatagtcg ttacgaagtg atatgatttc agcattcgtc ttgtaaagat tcttctctgc
     6721 gtcaggacgg gggccgccgc cctccgccgt ccgtgaaaaa cctgaatctg gtttacaata
     6781 aaccgtgaat atgcgagtta tatcgcacaa gggacatatg aaacacttca gcgtcatatt
     6841 gaaaagcctg gcggtcctta tgcttctggg actggcctcc tgcacaagca ttgatgcggt
     6901 caccggcgaa agggtctaca acctctacag catgcagagc gagatcaacc tcggcgtcca
     6961 gaccatccag gccaacacgg ccgaaatgca gaagcagggc tgccggatca acgacgatcc
     7021 caaacagatg gccaggctcg gcgaaatcac aaaacgcatc accaccgtgg ccgacatgcc
     7081 gcagctcccg tacgaagtca cgctctacca caccaacatc gtcaatgcgg ccgccgcgcc
     7141 gggcggacag atgtttgtgt tcgaagggct ctatgacaag gaaatcggcc tttgccgcga
     7201 tgacgacgaa ctggccgcgg tgatggcgca cgaaatcgcg cacgtcaact gccggcattc
     7261 gaccgagcga ctcagcaagc tgatgaccgc ggccgcggtg gttgaggtgg gcgcgatcgc
     7321 ccttgaaaac gagggcgaga gcgactgggc caccggcctg cgcgcggcct tcgccgtcgg
     7381 aaccgtcttc tatgtgccga tgcactccaa gaaggatgaa ttcgaggccg accgcgtggg
     7441 gcttttctac atggccaagg cgggctacga tccgcgcgcc gcgccgcgca tctggcggcg
     7501 cgtggccgag gaggatccgg acgagaagga caaggccagc atctttgcga cgcatccctc
     7561 ggccggcgac cgctgcgacg cgctggaggc gatggtgccc tatgccatgc aggaatacgt
     7621 gcgcgcgaca ggatcatatc cgaaggatta caagccggag aacgacggcc ttccggagtt
     7681 cgactggcgc cggcccggcg ccaggaaata aaagttccaa tggctggaac ttttgcgcaa
     7741 aaaacttcca atcattggaa cttttcagcc gaaaacttcc aatgactgga attaatgagc
     7801 ttcatcaggg aatgacaacg cggtacaagc ggtagtccgc agacactgcg tttgtgtaga
     7861 cattttcggg aggcgtcgga ctcagattcc ctgacagggc aatgaatccg tcctcaatga
     7921 tattcgtggt ggcttcaacc cggtaggggg atgaagggac gttcagccac gggctgttgg
     7981 tgcttgacca gcccatgctt ataccgccgg atgcgtcacg ctcaatccag gtcagctgaa
     8041 gaacagatgt ttcatctgtc ggatcggtcc cggccaagta ttcctcttca ttcggaacgc
     8101 cgtctgcatc gccgtgcccg tccgggtcgg tccccgtgcg ctggccgaac catgccagtt
     8161 cccaccagtc cggaagtccg ttgccgtcgg tgtcgccatc cagtcgtgtc agcgacaggt
     8221 ccgttggcgg attctcagca agcccccacc tcatcagtcc cgcgccgatg gtttgaaaag
     8281 aaccgtagcc gctggagtcc ccgccgtcta tgatgacctg atccacggga atggcgccgg
     8341 aggggttgga tacgcgcacg gtgccttgtc ctccggccgc gccgcggatg ttcaggcggt
     8401 ccaccgaggt gctggaggcc acatcgcagt catagaaact tccgggctgg aagacgacgt
     8461 tgcttgagaa agacaacgcg ccctgcgccc cggaactcga acccggcgag accgttccgg
     8521 acacgccaac aaggcctgcg gcgaccgtgc ctgtgccgcc caatgtgccg agtccatcaa
     8581 ccataacttc atcggattga agttcggcgc cgacccagag ggtgccgccc ttgatctgca
     8641 gctcggaggc cgtcagactt gccgccgtca gcaggcttgc cgcaaatatc atgaatcttg
     8701 tgatattcat ggttcccctg cctcaaggca tcgtgttcat ccctgtgttt tcagcctgca
     8761 tctgtcccgg cgcaatctcg ctcaggtttt gttcaaccgg cctctgttcg atatctgcgc
     8821 ggcatgccgg atcgttgcgg agcgccttga ccgtccagga caccttgatg tcctgtttcc
     8881 cggcaatggt aaaacggttg tccgcgacct cttccgcaac ccagacaaat gcctcgccga
     8941 ttggggtcag gctgtagatt tcagaaccgg ctttgttcag cgcggagtag taggccggaa
     9001 gttcaaccac cgctttcccg tc
//
LOCUS       DAOQWR010000107         9006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8225_length_9006_cov_5.962127, whole genome shotgun sequence.
ACCESSION   DAOQWR010000107 DAOQWR010000000
VERSION     DAOQWR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_9006_cov_5.962127"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PKK36_08775"
     CDS             complement(<1..1732)
                     /locus_tag="PKK36_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HNR94685.1"
                     /translation="MLKYTSMPVWRRIRRRLEFLYQEQAEQCLERFEMLVGRYGVGFN
                     HYPPFKYRDQSDSILITYGDMVRDGEAAPLETLENFLSDRLRGAVSTVHLLPFFPFSS
                     DDGFSVIDYRSVDPALGKWKHIDRFNENFSLMFDLVLNHVSRGSDWFKAYTRGIAPER
                     KYFLETDPSLDLSRTVRPRSGPLLSEVRTRSGVRHLWTTFSDDQLDLDFSNPDVLFEF
                     LDILLFYIAHGARIIRLDAIAYLWKRPGTSCIHLDETHEVVKLMRDLVELIGVDVSLL
                     TETNVPHAENIRYFGNGDEAHMVYQFPLPPLLLHALVTGDSRHLTQWAVNLEPPPEGC
                     SFLNFTASHDGIGVRPLEGLVSPEEMQSLLDHVRAAGGQISFKRNADGSESPYELNIT
                     YFDALGTPARPDERLQVERFLCSQTVMLALQGIPAVYFNSLFGGHNWTEGMQQTGRAR
                     TLNRKKWTQQEIAGMLDNPDGVPARIFRRCRSLLLLRAKHFAFHPAGMQTALDLGKEV
                     FLIVRTAPDKSESVVSISNFTAETVTRNPAPIIPRLKKAKEWHDLISGKNRKTAAGIE
                     LRPYETCWIRI"
     gene            complement(1732..2991)
                     /locus_tag="PKK36_08780"
     CDS             complement(1732..2991)
                     /locus_tag="PKK36_08780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94686.1"
                     /translation="MAERIGFISTRFSGTDGVSLESAKWAQVLWDYGHISYWYAGELD
                     RAEDVSLCIPEAHFKHPENLWINERVWGRETRDALVNRRILDMAAYLKSSLYAFMQKF
                     EISIFVIENCLSIPMHLPLGIAITEFLSETGMPSIAHHHDFYWERTRFSVSSVPGWLD
                     MAFPPRTPGMRHVVINLAAQEELAWRKGLTSLLIPNVLDFENPPPPPDAYASSVREDI
                     GLAPGDVMILQPTRVVPRKGIEHAIKLVGMLGDPKYKLVISHKAGDEGMEYCQMLAEM
                     AREEGVDLRFVETRVGDSRQFDPQGKKIYTLWDLYSHADLITYPSLYEGFGNAFLEAI
                     YFRKPVLINRYAIFGRDVEPKGFRVPVMDGYLTKGVVRELRKILEDAEYRRQMVDHNY
                     QVATRFYSYSVLRRSLRTLITDIRGLD"
     gene            3124..4299
                     /locus_tag="PKK36_08785"
     CDS             3124..4299
                     /locus_tag="PKK36_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HNR94687.1"
                     /translation="MAKKKFQEILDRLPALLSQAGSCVPALLSNLVLIGEIPSPTFGE
                     ERRVRFISDRFAENGLVSGSVDEKGNAVAVLPGGRGDRSILLVAHADTGAPANVDHTI
                     LIEPGQVTGPGVANNSLGVAVLAALPHFLEEMDVALDANLIFLAASRNLARGNLEGLR
                     FFLQNNSQPVDAGICIQGTQLGHLSIQSLAMMRGEITCTVPEEYNWTRFQATGAIRTI
                     NEVINRINEIPLPNKPRSNIAFGSINGGSGFHRVARHAVLRFEVRSEDDAMIRKIVRQ
                     IRDICEDVSSQKESRIDLDVFAERKAGGIPFAHPLSRRARKILEILEVPPRVSPSTSE
                     IFAFIERGIPALSLGISTGESLAFPDERVDVPPMAAGMAQLVALLLAIDGGFCDEHP"
     gene            4286..5263
                     /locus_tag="PKK36_08790"
     CDS             4286..5263
                     /locus_tag="PKK36_08790"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNR94688.1"
                     /translation="MNIHEWLKNNTFHYRQFWDLKHLVAEKEKRGLRVSLCIPTLNEE
                     KTIGKEVIVFRSELMTRYPLVDELAVIDSGSTDKTREVAAQFGADVYLASDILPEAGE
                     KRGKGENLWKAIYQLTGDIIVYIDADIKNIRPHFVYGLVAPLIYRPEIKYVKAFYERP
                     LAAANGVRPIGGGRVTEILIRPLFSLFFPDLAGLIQPLSGEYAVRRDVLEQLPFPIGY
                     GVETSHLIDVYKKWGLQAIAQTDLDQRVHRNQPTRDLGKMAFGILQTFLSRARRMGIV
                     GNLPELGTVLRQFQVQGDEYKIQEFDLHEEERPPMIEMPAYRRRRGLDG"
     gene            5268..6695
                     /locus_tag="PKK36_08795"
     CDS             5268..6695
                     /locus_tag="PKK36_08795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR94689.1"
                     /translation="MRVAVVHYHLRIGGVTTVIDQAARALGALGVQVCVLAGERPAVE
                     LAAPAAVIPGLAYGRHELSASDILKQADEVASRLLGGAPDVWHIHNHSLGKNLVVPRL
                     ACALASAARPLVLQMHDFAEDGRPANYELLLAGLADGEPATLGPALYPRASHVHYAFL
                     NRRDQHAFMRAGLPESRAHYLPNAVVSPPSAGEAPPRTDLVIYPTRAIRRKNVGEMVF
                     WAALDRSGRRYALTRAPRNPEHAAGYNRWVDFSAAHRLPVEFGLAEKSPLSFPELMRS
                     AACAITTSVAEGFGLAFLEPWLMDCPLTGRALPEIAGEFKNDGLDLSGLYDRLDVPLK
                     WAGAGNLRRALQDAMGRVYASYHRELLPQDVEQAFNAAVQNNFADFGRLDESMQQQVI
                     EHILNNPADARDIRPSALNIPDAVVPFNKNTVLEKYGPARYGERLREIYRAAAESGPA
                     HEDALDAESILDQFLLPERFNLLRT"
     gene            6699..7604
                     /locus_tag="PKK36_08800"
     CDS             6699..7604
                     /locus_tag="PKK36_08800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR94690.1"
                     /translation="MKDASGNKFLIRRIRELSRPMTVESTGLESDIRPLKGIRAVLFD
                     VYGTLLISGSGDVGTAAASPSDAAMHVALTVAGCEPEPAAGPRGAELMLQNIAAAHAR
                     KKAAGIEFPEIAICEIWEMILARLLKERLIGLRPADALLEPLAVEYECRVNPVWPMPG
                     FPEVLRELDARIEAMGIVSNAQFYTPLAMEALSGFPVDQLGFRTDLSVYSFRAGRAKP
                     SPQLFQQVLVPLERNYGIAASEALYVGNDMLNDIQTAARAGCRTALFAGDARSLRLRN
                     QKPADLYPRPDLIITNLLQLPRALA"
     gene            7902..8813
                     /locus_tag="PKK36_08805"
     CDS             7902..8813
                     /locus_tag="PKK36_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR94691.1"
                     /translation="MKKMLLLVAIALVSTSLFAAPLRVLLLDFENAAGGTAALTNPSQ
                     IAGKGVYLLSKQLLDNENFVLVDRREFISQMEKLKLTDAGAATPVQPTFIHAAQQLGA
                     DVILRGTLLSLSEGKETVDQGGYRTDFTVLTSRVALEALDAKDGSVIAMSEGSSSRKI
                     RQTATQQTSLGEDEVLSLLDKAVAQAVPSVQQALESRMVTADLSKIKISIKTTDDPAM
                     VEIDGSLVGSTPLEGFEIAAGDHVLRVSKAGYRDITRRIVLEKDASIEVPMMRVELDA
                     LEQKEVLKEADVHIFSREPDLIIHTIE"
     gene            8827..>9006
                     /locus_tag="PKK36_08810"
     CDS             8827..>9006
                     /locus_tag="PKK36_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94692.1"
                     /translation="MAEFLQTVQNGLAWILFAGVSAAALFLSCLSISGTWLAALLAAW
                     AVFWKTGRVGWGTVAV"
BASE COUNT         1812 a         2581 c         2704 g         1909 t
ORIGIN      
        1 cgatccggat ccagcaggtt tcgtacggcc gcagttcgat ccctgccgcg gtttttcggt
       61 ttttcccgct gatgagatcg tgccactctt tcgccttctt cagccgggga attatcgggg
      121 cgggatttcg cgtgacggtt tcggccgtga agttgctgat ggataccacg gactctgatt
      181 tgtccggcgc cgtgcggaca atgagaaaca cctctttccc gaggtccagc gcagtctgca
      241 ttccggcggg atgaaacgcg aagtgctttg cccgcagcag gaggagtgat cggcagcggc
      301 ggaatatccg tgccgggacc ccgtccggat tgtccagcat gccggcgatt tcctgctgtg
      361 tccatttttt ccggttcagg gttctggcgc ggccggtctg ctgcattcct tcggtccagt
      421 tgtgtcctcc gaacaggctg ttgaaataga cggctggaat gccctgcagc gcaagcatga
      481 ccgtttgcga acagaggaag cgttcgacct gcagcctctc atccggccgg gccggagttc
      541 cgagcgcatc aaaataggtg atgttcagtt cgtaggggct ttcagatccg tcggcattgc
      601 gcttgaagga tatctgtccg cctgccgccc ggacatgatc aagaagcgac tgcatttcct
      661 cagggctgac caaaccttcc agcggcctca cgccgatgcc gtcatgggat gcggtgaagt
      721 tgaggaagga gcagccttct ggcgggggct cgagattcac ggcccattgc gtgagatgcc
      781 ggctgtctcc ggtgaccagc gcatgcagca gcaggggggg cagcgggaac tggtagacca
      841 tgtgagcctc gtctccgttc ccgaaatagc ggatattctc cgcatgcggg acattcgttt
      901 ccgtcaggag ggagacgtca acgccgatga gttcaacaag atcccgcatc agcttcacca
      961 cctcgtgtgt ttcatcgaga tggatgcagg aggttccggg ccgtttccag agataggcaa
     1021 tggcgtccag ccggatgatg cgcgcgccgt gggcgatata gaacaggagg atgtccagaa
     1081 actcgaacag cacatccgga ttggaaaagt cgaggtcgag ctggtcgtcg ctgaacgtgg
     1141 tccagagatg gcggaccccc gaccgcgtcc gaacttcgct cagcagcggt ccgctgcgcg
     1201 gccgcacggt ccggctgagg tccagcgagg ggtccgtttc caggaagtat tttctctccg
     1261 gggcgatgcc gcgcgtatag gctttgaacc agtcgcttcc gcgtgaaaca tgattcagca
     1321 ccaggtcgaa catcagagaa aaattctcgt tgaaacggtc tatatgcttc catttcccca
     1381 gtgcgggatc cacggagcgg tagtcaatga cagaaaagcc gtcatcggat gagaagggga
     1441 agaacggcag caggtggact gtgctgacgg ccccgcggag cctgtctgaa agaaaattct
     1501 ccagtgtttc caggggtgcg gcctcgccgt cgcgcaccat gtcgccgtag gtgatcagaa
     1561 tgctgtcgga ctggtcgcgg tatttgaagg gcgggtagtg gttgaacccg acgccgtaac
     1621 ggccaaccag catctcgaag cgctccaggc actgttcggc ctgttcctga taaagaaatt
     1681 ccagccgccg ccggattcgc cgccacacag gcatggaggt gtatttcagc atcagtccag
     1741 cccccggatg tccgtgatca gtgtgcgcag gctccggcgc agcacagaat agctgtagaa
     1801 gcgcgtggcc acctggtaat tgtgatccac catctgacgg cggtattccg cgtcctcaag
     1861 aatcttccgc agttcgcgga caacaccctt ggtgagatat ccatccatga ccggcacgcg
     1921 gaagcccttg ggttcaacat cccggccgaa aatggcatac cggttgatca gcaccggctt
     1981 ccggaagtag atggcttcca gaaaggcgtt tccaaagccc tcataaaggc tgggataggt
     2041 gatcaggtcg gcgtgggaat agagatccca gagtgtatag attttcttgc cctgaggatc
     2101 aaactgacgg ctgtcgccga cccgcgtttc cacaaagcgg agatccaccc cctcttcgcg
     2161 cgccatttca gcaagcatct ggcagtattc cattccctcg tctcccgcct tgtgcgagat
     2221 caccagcttg tatttcggat cgccgagcat tccgaccagt ttgatcgcgt gttcgatgcc
     2281 tttgcgggga accacgcgtg tcggctgaag gatcatgaca tcccccggag ccagcccgat
     2341 gtcctcccgc acgctggagg cataggcatc cggaggcggc ggcggatttt cgaagtccag
     2401 cacgtttgga ataagaagag acgtcaggcc cttgcgccac gccagttcct cctgcgcggc
     2461 gagattaatc accacatggc gcatgccggg cgtgcgcggg ggaaaggcca tgtccagcca
     2521 tcccggcacg gaactcacgg agaaccgtgt tctttcccag tagaagtcgt gatggtgcgc
     2581 aatggagggc atgcccgtct ccgacaggaa ttccgtgatg gcgatgccca gcggcagatg
     2641 catgggaatg ctcaggcagt tttcaatcac gaagatgctg atctcaaact tctgcatgaa
     2701 ggcgtacagc gagcttttca gataagcggc catgtcaaga atccggcggt tcacaagcgc
     2761 gtcgcgagtt tcccggcccc acacgcgctc attgatccag agattttccg gatgcttgaa
     2821 atgtgcttcg gggatgcaga ggctcacatc ctccgcccgg tcaagctcgc cggcatacca
     2881 gtagctgatg tgcccgtagt cccagagcac ctgcgcccac tttgcgcttt cgagactgac
     2941 gccgtccgtg cccgaaaaac gcgtggaaat gaaacctatt ctctcagcca ttgttgtatt
     3001 tttgcctttt cccctggtct ggttcagttt attcttttac tgaatgctac gggacatccg
     3061 cccttattgc aagcgagttt ggccggccga ggccttctgc tcaaacagct ggatgtgaaa
     3121 aatatggcaa agaaaaaatt tcaggaaatt ctggaccggc tgccggctct cctttcacag
     3181 gccgggagct gtgtgcccgc gctgctttcc aatctggtgc tgatcggaga aattccgtct
     3241 cccacatttg gagaagaacg ccgggtacgg ttcatcagcg accggttcgc ggaaaacggc
     3301 ctggtcagcg ggtcggtgga cgaaaagggc aatgccgtgg ccgtgctgcc cggcgggagg
     3361 ggggaccgca gcatactgct ggtggcccat gcggataccg gcgcccccgc caacgtggac
     3421 cataccattc tgatcgagcc gggccaggtg accgggcccg gagtggccaa caacagcctc
     3481 ggtgtggcgg tgctggccgc gctgccccat tttctggagg aaatggatgt cgcgctggat
     3541 gccaacctca tatttcttgc cgcttcgcgc aacctggccc gcggcaatct tgagggcctg
     3601 cggtttttcc ttcagaacaa ttcacagccc gtggacgccg gcatctgcat tcagggaacc
     3661 cagctggggc atctgagcat ccagtccctg gccatgatgc gcggagaaat cacctgcact
     3721 gttccagagg aatacaactg gacacgcttc caggccaccg gcgccatccg cacgatcaac
     3781 gaagtgatca accggatcaa cgaaatcccc ctgccgaaca agcctcgctc aaacattgcg
     3841 ttcggttcaa tcaacggcgg gagcgggttc cacagggtgg cccgccacgc ggtcctccgc
     3901 tttgaagtgc gcagtgaaga tgatgcgatg atccgcaaga tcgttcgtca aatccgggat
     3961 atctgcgagg atgtgtcctc gcagaaggag agccggatag accttgatgt gtttgccgaa
     4021 cgcaaggccg gcggcattcc ctttgcccat ccgctttcgc ggcgcgcgcg gaagattctg
     4081 gagattctgg aggtccctcc ccgggtcagt cccagcactt cggagatctt tgcgttcata
     4141 gaacgcggca ttccggcgct gagcctgggc atttccacgg gcgaaagtct cgcttttcct
     4201 gacgagcgcg tggatgtccc ccccatggcg gcgggcatgg cccagctggt ggctctgctg
     4261 ctggctatcg acggaggttt ttgcgatgaa catccatgaa tggctgaaaa acaacacctt
     4321 ccactaccgc cagttctggg atctgaagca ccttgtggcc gaaaaggaga aaaggggatt
     4381 gcgcgtttcc ctgtgcattc ccacgctgaa cgaggagaaa acaatcggga aggaagtgat
     4441 tgttttccgc tcggagctga tgacccggta tccgctggtg gacgagcttg cggtcataga
     4501 ctccggatcg accgacaaaa cgcgcgaagt ggccgcgcag tttggggcgg atgtgtatct
     4561 ggcgtctgat atccttcccg aggccggtga aaaaagggga aagggcgaga atctctggaa
     4621 ggccatctac cagctgaccg gggatatcat cgtttatatt gatgccgaca tcaaaaacat
     4681 ccgccctcat ttcgtgtacg gcctggttgc gccgctgatc taccgccccg aaatcaagta
     4741 tgtgaaggcg ttctatgaac ggccgcttgc ggccgccaac ggcgtgcgtc cgattggcgg
     4801 cggccgcgtc acggaaattc tgattcggcc gctcttctcc ctctttttcc ccgatcttgc
     4861 cgggctgatc cagccgctat cgggggaata tgctgtccgg cgcgatgtgc tggaacagct
     4921 cccgtttccc atcggatacg gcgtggagac ttcgcacctg attgacgtct acaagaaatg
     4981 gggacttcag gccattgctc agaccgacct cgatcagcgg gtgcaccgca accagcccac
     5041 gcgtgatctc gggaaaatgg cgttcggcat tctgcagacc ttcctttcgc gtgcccggag
     5101 gatggggatt gtgggcaatt tgcccgaact gggcacggtc ctgagacagt tccaggttca
     5161 gggcgatgag tataaaattc aggaatttga tctgcatgaa gaggaacgcc cgccaatgat
     5221 cgaaatgccc gcctatcgtc gccggcgggg actggacggc tgactgcatg cgcgtggcgg
     5281 ttgtccatta tcacctccgc atcggcgggg taaccacggt gattgaccag gccgcgcgcg
     5341 cgctgggcgc cctgggtgtt caggtctgcg tcctcgcggg cgagcgcccg gccgttgagc
     5401 ttgcggcccc cgccgcagtg attcccgggc ttgcgtacgg ccggcatgaa ctcagcgctt
     5461 ccgatattct gaaacaggcg gacgaagtcg catcccgttt gctgggcggc gcgcctgatg
     5521 tctggcatat acataatcac tccctcggca agaatcttgt tgtgccgcga ctggcctgcg
     5581 cgctggcgtc cgccgcccgt ccgctggtgc ttcaaatgca tgactttgcc gaggacggcc
     5641 gcccggccaa ttacgagctt ctgctggcgg gcctggctga tggagaaccc gccacactgg
     5701 ggccggctct ttatccccgc gcaagccatg ttcactatgc cttcttgaac cgccgcgacc
     5761 agcacgcctt catgcgggcc ggccttcccg aatcgcgcgc gcattatctt ccgaatgcgg
     5821 tggtttcccc tccctcagcg ggggaagctc ctccgcgcac tgatctggtg atctatccca
     5881 cccgcgccat acgaagaaaa aatgtcggcg aaatggtgtt ctgggcggct cttgaccgct
     5941 ccggccggcg ttatgccctg acccgcgctc cgagaaaccc ggagcatgcc gcggggtaca
     6001 accgctgggt tgatttttcc gccgcgcacc ggctccccgt ggagttcggc ctggccgaaa
     6061 aatccccgct gagttttccg gagctgatgc gcagcgccgc gtgtgccatc accaccagtg
     6121 tggccgaagg ctttgggctg gcctttctgg agccatggct gatggactgc cctctgaccg
     6181 ggcgggcgct tcccgagatt gccggtgaat tcaaaaacga cggactggat ctcagcggtc
     6241 tgtatgaccg cctggacgtt cccctgaaat gggccggcgc cgggaacctg cggcgcgccc
     6301 tgcaggacgc catgggacgg gtttatgcga gttatcaccg ggaacttctg ccgcaggatg
     6361 ttgaacaggc ctttaatgct gcggttcaaa ataattttgc ggatttcggg cggcttgatg
     6421 aaagcatgca gcagcaggtg attgaacata ttctgaacaa tcctgcggat gcgcgtgaca
     6481 tccggccctc tgccttgaac attccggatg ccgtcgtccc gttcaataaa aacactgtgc
     6541 tggaaaagta tggtcccgcc cgctatgggg agagactgcg ggagatatac cgcgcggcgg
     6601 cagaatcagg gcctgcgcat gaagacgcgc tggatgccga aagcatactt gatcagtttc
     6661 tgctccccga acgcttcaat ctgctgagga cctgaaaaat gaaggatgca tcgggaaata
     6721 aatttctgat ccgccggatt cgggagcttt cccgtccgat gacggttgaa tcaaccgggc
     6781 tggaatccga catccggccc ctgaagggca ttcgagccgt gctgtttgat gtgtatggaa
     6841 ccctgctgat atccggcagc ggagacgtgg gaacagcggc cgccagtccg tcagatgccg
     6901 ccatgcatgt cgcgctgacc gtggccgggt gcgagccgga acccgccgcc ggaccccggg
     6961 gggctgagct gatgcttcaa aatatagcgg cggcgcatgc gcggaagaag gccgcaggaa
     7021 ttgagtttcc tgaaatcgcg atctgcgaga tctgggagat gatacttgcc cggctcctga
     7081 aagagcgtct gatcggtttg cggccggcgg atgcgctgct ggagccgctg gcggtcgagt
     7141 atgagtgccg cgtgaatccc gtctggccca tgccgggatt cccggaggta ctgcgggaac
     7201 ttgacgcgcg gatcgaagcg atggggatcg tttcgaatgc gcagttttac accccgctgg
     7261 ccatggaggc cctctccgga tttccggtgg atcaactggg ttttcggaca gatctttccg
     7321 tttattcttt ccgggcgggt cgggccaagc cctccccgca gctgtttcag caggtgctgg
     7381 ttccgcttga aagaaattac ggaatagccg cttctgaagc tctttatgtg ggcaacgaca
     7441 tgctgaatga tattcagact gccgcccggg ccggctgcag gacggctctt tttgcgggtg
     7501 atgcccggtc gctgcgctta agaaatcaga aacctgcgga cctgtatccg cggccggatt
     7561 tgatcataac caacctcctt cagcttccgc gcgcactggc ctgaaatcgt aaaaaacctc
     7621 caaaaatcgt taaaaaaggc caaaatttgt caaaaaatgg ccaaaaatca tcaaaaatcg
     7681 tcaaaaaacg gccaaaattc gcaaaaattc gaaaaattca gacaaaattc gaaaaattcc
     7741 gctttccccg caaaaaaaca atgttcggcc ggagaataat atttcgcgcg gttttgcgtt
     7801 atctgtaaga gaacgaacgc ggccggacga gcggcgcttt ctcttgattc tgccaatggg
     7861 tgagcatacg attcggtcaa ggttttgaaa ggagttcaat tatgaaaaaa atgcttttgc
     7921 ttgttgcgat cgctctggtc tcgacctcgc ttttcgcggc cccgctgcgc gtgcttctgc
     7981 ttgatttcga aaatgcagcc ggcggaaccg ccgcattaac caatccttcc cagattgccg
     8041 gcaaaggtgt ttatctgctt tctaaacagc tgctggacaa cgagaatttt gtgctcgttg
     8101 accggcgcga gtttatcagc cagatggaga aactgaagct cacggatgcg ggggccgcca
     8161 cgcccgtgca gccgacgttc attcatgccg cgcagcagct gggcgctgat gtgattttgc
     8221 ggggcacgct gctgtcgctc tccgaaggga aggaaaccgt ggatcagggc ggataccgaa
     8281 ccgacttcac cgtgctgacc tcccgtgtgg cgctggaggc gctggatgca aaagacggaa
     8341 gcgtgattgc catgagcgaa ggctcctcca gccgcaaaat ccgccagacg gccacccagc
     8401 agacctcgct gggcgaggat gaagtgctgt ccctgctgga taaggccgtt gcgcaggccg
     8461 ttccaagtgt gcagcaggcc ctggaaagcc gcatggtcac agccgacctg tccaaaatca
     8521 aaatctccat taagaccacg gatgatcccg ccatggtgga aatagacgga agccttgtgg
     8581 gttccactcc gctggaaggt tttgaaattg cggcggggga tcacgtcctg cgtgtgagca
     8641 aggccggcta tcgcgacata acgcgccgaa tcgtgctgga gaaggatgcc tcgattgaag
     8701 ttcccatgat gcgcgtggaa ctcgatgcgc tcgagcagaa ggaagtgctc aaggaggccg
     8761 acgtgcacat cttctcccgc gagcccgatc ttatcatcca taccatcgag taggcggatt
     8821 tcccctttgg cggagtttct gcagactgtt cagaacgggc tggcatggat tctgtttgcc
     8881 ggcgtgagcg ccgcggccct gtttctctcc tgcctgtcca tttccggcac ctggctggcc
     8941 gccctgcttg cggcctgggc cgtgttctgg aaaacgggcc gggtcggctg ggggaccgtg
     9001 gctgtt
//
LOCUS       DAOQWR010000108         9002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8228_length_9002_cov_6.759808, whole genome shotgun sequence.
ACCESSION   DAOQWR010000108 DAOQWR010000000
VERSION     DAOQWR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8228_length_9002_cov_6.759808"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKK36_08815"
     CDS             complement(<1..372)
                     /locus_tag="PKK36_08815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNR94693.1"
                     /translation="MNTLSILNLLAGLILAPLLPGIINRTKAVFAGRHGQPVLQTYFD
                     IFKLLRKGAVYSRTTSWVFRLSPVIGLAAVIAAMTLLPALSAPAPVSFAGDLILFAYC
                     FGLLRFFLVAAALDTGSAFEGM"
     gene            complement(379..2358)
                     /locus_tag="PKK36_08820"
     CDS             complement(379..2358)
                     /locus_tag="PKK36_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNR94694.1"
                     /translation="MSLLLLPLIIFFAGALLQMVFGRRVAVVRVAGPAITVAGALAGL
                     IPVIRAFGGKSVTASLPWSMPFGSFSVEMDGLSALFALPILVICALAAVYGSEYLKSF
                     EGKKNLAGFWFFFNVLAGSMLLVVTARNAILFLLAWEVMSLSSFFLVIFESEQEHARR
                     AGWTYLVATHIGTAFLLVLFCMLGAQAGTLDFSGFSAAGMAPKFLSAAFLLAVIGFGT
                     KAGFMPMHVWLPEAHPAAPSHVSAVMSGVMIKTGIYGLLRVLTHLGTPPAWWGWVLVA
                     IGAASGILGIVFALSQHDIKRLLAYSSVENVGIITAGIGIGMLGMSYGNDAMMLLGFA
                     GALLHVLNHSIFKSLLFLGAGSVLHAAATREIDRLGGLMKRTPFTGAAFLAGSVAICG
                     LPPLNGFLGEFMIYLGSFKALTGSAAACPAAGVVVIAALALIGGLAAVCFTKAFGMIF
                     LGEPRSDAAAHAHECGPAMRIPMLVLAALCVAVGLAGPFIPKLLIPAVQVLAGRQMTD
                     GNFFAPTERLLTNISLFATGVLALSALLLVLRTRLLRNKPVSESVTWDCGYAAPTARM
                     QYTASSYADPVVRLFKLFLQTRRKFVSPKGLFPEKSSFSSATPDFYRERMYRPAFNAT
                     EKFLTRFQWLQHGRLNLYILCIMVTLIVILVWKLR"
     gene            complement(2359..3048)
                     /locus_tag="PKK36_08825"
     CDS             complement(2359..3048)
                     /locus_tag="PKK36_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNR94695.1"
                     /translation="MQLNVREAACLLAVPEKTVLRWVRNDGLPAFKINEQYRISRSDL
                     LEWTQARGIPVSAKLEPASTAIVSLADALTNGDVHFDVGGTDLESVLNAAVETMPLPE
                     DVDRDFLLRVLLARESLGSTAIGNGVAIPHVRNPIVLNIRQPIISLCFLKQAIEFGAL
                     DGIPVHTLFVIVSPTINTHLSLLARLSYALKQPAFSDAVARKASRDEIIACAGQIDTM
                     LPAHTAQTGAR"
     gene            complement(3502..4680)
                     /locus_tag="PKK36_08830"
     CDS             complement(3502..4680)
                     /locus_tag="PKK36_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNR94696.1"
                     /translation="MKYQIHAVSELVGALRDGPALIQIMTGPRQVGKTTAAMDVQNTL
                     GWPTVFEAADYFPAPGPEWIETQWMRARALPGPRRLLVLDEIQKVRGWSETVKRLWDE
                     DRRHKFPLSVLLLGSSALLLHRGMSESLAGRFLLHRCMHWTYPECSRAFGWNLDQWLY
                     FGGYPGAAAVADNEAQWARYVSDSLIETAIARDVLQMHPLNKPALLRALFGVAASHPA
                     QIFSYNKMIGQLQDAGNTTTLAHYLELLETAYLASGLSQYSAGHPRKRGSSPKLILWN
                     NALINAFSQRSFDQSKNDGAWWGRLVENAAGAHLLNHLPRERYSVNYWRQGNDEVDFV
                     VSTGKLIWAIEVKSGRPGRTGGLEAFRARYPRSRALIVGSGGIPLAEFFSTAASQWFQ
                     "
     gene            5176..5439
                     /locus_tag="PKK36_08835"
     CDS             5176..5439
                     /locus_tag="PKK36_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR94697.1"
                     /translation="MIAVATTKMSSKGQVVIPEELRNRMNLSAGSQFVVVGEGDVVIL
                     KTLTVPSMKQFDRLIRKARRQARTAGMKPRDVERAIAAVRGRQ"
     gene            complement(5640..6404)
                     /locus_tag="PKK36_08840"
     CDS             complement(5640..6404)
                     /locus_tag="PKK36_08840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNR94698.1"
                     /translation="MTAALKLRLPMKADSTTEGILEFGSRRIPYSLIRGLRKRVRIAV
                     TPDMAVTVSAPVRARDAQIEELLHKKAHWIVRTLDRIKTFHPLPAPKQYVSGETLLYL
                     GRQYRLKIVQGEPAPARLIGRFLLVSVADKGSSCKAKRSVENWYRERAKDTFRRYLEK
                     CTKVTARHGVPPAEITLRKMYTRWGSCSRKGRITINTNLVQAPVHCIEYVIMHELCHL
                     KHHNHSRGFYHLLAQCMPDWPKRKNALASLVIPHDK"
     gene            complement(6364..>9002)
                     /locus_tag="PKK36_08845"
     CDS             complement(6364..>9002)
                     /locus_tag="PKK36_08845"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNR94699.1"
                     /translation="AQLADYQAPDGIPQLFLYSQLLLALARDKAEYGTTGTPRKFWSG
                     WREENADAEIRDLIGTTLPDDQLNKLLDDGPFKANRQAFEKLLKEGRMIYEQDRTLYG
                     MCRPDRLLELMYQFILFDNGIKKIARYQQFFTVKDILKRVCAAKNGEPRNGGVVWHTQ
                     GSGKSLTMVMLAKALALSKAIESPRIILVTDRVDLDDQIWGTFAACGLKPEQANTGAH
                     LVEMLTDGKRHVITTLIHKFAAATAGSGLKEADPNTFVLVDESHRSNYSEHHARMKLA
                     LKGACFIGFTGTPLAKDPKKNTFAQFGELFKPAYTISRAVRDKAVVPLVYEARHIPQP
                     VQKAEIDGWFERLTSPLNEEQKADLKRKFSSEKQLNKAAQKVRMVAWDVSLHYSMAYQ
                     GTGMKGQLVAPDKETARLYKKLLDEFGMVKSEVLVSGPAVREGEDKGTEASEDEKVFW
                     KAMMDRYGSEEKYNKQLINAFKHGEEPEIIIVVDKLLTGFDAPCNTVLYLARSLKDHT
                     LLQAIARVNRLYEGKEYGLIMDYSGVIENLDEAIDFYSRLADYDHADLVETVTFITDQ
                     AARLPQLHSDLWELFAGVRGSTDPEVFQEHLRDEERRSKFYDRFSAFARAMGLALAST
                     TFLEQTPEKQINIYKKDLKFFRNLRADASIRFQERVDFSEYEPKIKKLLDTHVGAGEA
                     EQLIEPIDLLNTEQREQVLEKEGQSAESKADMIASATRHTIEHEFEKDPVFYQKFSQL
                     LETVLAALHEKRLQAAEALERIKDIAVKVATHTGDDVPEMLVGNDMARRYFGTVNEEI
                     AQYTTDKPDVGATIALEVAKRIDRHKVRDWRDNHDALNRMRREIDSIFFEVAKEFGLS
                     FPLEVQDKLIDRCIEIAIANES"
BASE COUNT         2068 a         2480 c         2524 g         1930 t
ORIGIN      
        1 catgccttca aacgccgaac cggtgtccag cgcggcggcc accaggaaaa agcggagcag
       61 gccgaaacag taggcaaaga ggatcaggtc tcccgcgaaa gaaaccggcg caggggcgga
      121 cagggccggc agaagcgtca tggcggcgat gactgcggcg aggccgatga ccggagacag
      181 gcggaaaacc caagaggtgg tccggctgta cacggcgccc ttgcgcagga gcttgaaaat
      241 atcaaaatac gtttgcagaa ccggctgccc gtgccggccc gcgaatacgg ccttggtgcg
      301 gtttatgatg cccggcagca gcggcgccag gatcaggccg gccagcagat tgaggatgga
      361 aagtgtgttc atttccattt acctcagttt ccacaccagt atgacaatga gcgtgaccat
      421 gatgcacagg atataaagat tcagccgtcc atgctgaagc cactggaagc gggtcagaaa
      481 cttttccgtc gcgttgaaag cggggcgata catgcgctcc cggtagaaat cgggcgttgc
      541 gctggagaac gatgattttt ccgggaacag gcccttcggc gagacaaatt tcctccgagt
      601 ctggaggaaa agtttgaaca gacggacaac gggatcggcg taagaggagg ctgtgtactg
      661 catgcgcgcg gtcggggcgg cgtaaccgca gtcccacgtc acggactccg agacgggttt
      721 gttcctcagc aacctggtgc gcagtacgag caggagcgcg ctcagcgcca gcacccctgt
      781 tgcaaacagg gatatgttcg tgaggaggcg ctcggtcggc gcgaagaaat tgccatccgt
      841 catttgccgg cccgccagca cctgaacggc agggatcagc agctttggaa tgaacggacc
      901 ggcgagcccg accgcaacgc agagcgccgc gagaaccagc atcgggattc gcatggcagg
      961 tccgcattca tgcgcgtgcg ccgcagcatc gctgcgcggt tcgccgagaa agatcattcc
     1021 gaaagccttg gtgaagcaca cggcggcgag acccccaatc agcgccagcg cggcaataac
     1081 gacaacgcct gccgccgggc atgcggcggc agatcccgtc agggccttga atgatccgag
     1141 gtagatcatg aactcgccca ggaatccgtt cagcggcggc agtccgcaga tcgcaaccga
     1201 tccggcaaga aacgccgctc cggtgaaggg cgtgcgtttc atcagtccgc caaggcggtc
     1261 aatctcgcgc gtggccgccg catgaagaac ggacccggcc ccgaggaaaa ggaggctctt
     1321 gaagatcgag tggttgagca catgcagaag cgcgccggcg aaaccgagca gcatcatggc
     1381 gtcatttccg taactcatgc ccagcatccc gatgccgatg ccggcggtga taatgccgac
     1441 attttccacg ctgctgtagg ccagcaggcg cttgatgtca tgctgcgaaa gagcgaagac
     1501 aatgcccaga atgccggatg ctgcgccgat ggcaacaaga acccagcccc accacgcggg
     1561 cggagttccg agatgggtca ggacgcgcag cagaccgtaa attcccgtct tgatcatgac
     1621 tccgctcatc acggctgaga catgagaagg ggccgccgga tgcgcctcgg gaagccagac
     1681 gtgcatgggc atgaagcccg ccttggttcc gaagccgatc actgccagca ggaacgccgc
     1741 tgacagaaac ttcggggcca tgcctgcggc ggagaagccg ctgaaatcca gcgtgccggc
     1801 ctgcgcgccc agcatgcaga agaggacgag gagaaaagcg gttccgatgt gcgtggccac
     1861 cagataggtc cagccggcgc gccgcgcgtg ttcctgttcg ctttcaaaaa tcacaaggaa
     1921 aaaggaggaa agcgacataa cctcccacgc cagcagaaaa aggatggcgt tgcgcgcagt
     1981 caccaccaga agcatgcttc cggccagcac attgaaaaag aaccagaagc ccgcaaggtt
     2041 ctttttgccc tcgaatgatt tcagatattc cgaaccatag accgcggcca gtgcgcagat
     2101 aaccagaatg ggcagggcaa acagggcgga aagaccgtcc atttcaacag aaaatgatcc
     2161 gaacggcatg ctccatggaa gcgaggctgt gacggatttt ccgccaaaag ccctgatcac
     2221 tggaatcagg ccggccagtg cgccggcaac ggttatggcc gggccggcca cgcggacgac
     2281 agcgacgcgg cggccgaaaa ccatctgcag aagcgctccg gcaaaaaaga tgatcagcgg
     2341 cagcaggagc aggctcattt atctggctcc ggtttgcgct gtatgcgcgg gcagcatggt
     2401 gtcaatctgc ccggcgcatg caatgatttc atcccgggat gccttgcggg ccacagcatc
     2461 ggaaaatgca ggctgtttca aggcatagga tagacgcgcg agaagactca ggtgagtgtt
     2521 gatggttgga ctcacgatca caaagagcgt atggaccgga attccgtcca gagcgccgaa
     2581 ttcgatggcc tgtttaagga agcaaagaga aatgatcggc tggcggatgt tcaggacaat
     2641 cgggttgcgc acatggggga tggcgacccc gttgcctatg gccgtggaac cgagcgattc
     2701 ccgcgcaagc agcacgcgca ggagaaaatc cctgtccaca tcctcgggca gaggcatggt
     2761 ttcgaccgcg gcgttcagca ccgactccag atcggttccg ccgacatcga agtgaacgtc
     2821 gccgtttgtc agcgcatcgg cgagcgaaac gatggctgtt gaagccggct ccagtttggc
     2881 ggaaacgggg attcccctgg cctgggtcca ctcgaggagg tcggaacggc tgatgcggta
     2941 ctgctcgtta atcttgaatg caggcagtcc gtcattccga acccatcgaa ggacggtctt
     3001 ctcaggcacc gccagcaggc aggcggcctc ccttacattc agttgcataa tcgctcagaa
     3061 actccattcc ggataaaagc ggacaaacag tagcaaaccg ccttgtggaa tcaatagaaa
     3121 tccggaccgg cgtctaaaaa tgctcaaaaa ttgtcaaaaa caggccgaaa acgtccaaaa
     3181 agtgccaaaa aacgtcaaaa aaggccaaaa aacgactaaa attcaagaat tcatgaaaat
     3241 atttcgcaca aaggcacaga gacacaaaga ccccggaaag tattcatcat cttcatgaaa
     3301 ttcatgctct tcatggtaaa acatcttttg aaaacctgac ggcttcgcgc ccgtttattc
     3361 gcggttcatt ttgggttgtg aatccgccgc actgggctct tcatggtaaa atcttttctg
     3421 ggcccctgaa ccggcgttca gaaaaactcg cgggtggttt tgtccgaaca aaattatgac
     3481 ttgcagggac tcaacccctc ttcactggaa ccactggctg gctgcggtgc tgaaaaactc
     3541 ggcgagcgga atccccccgg atccaacgat aagggcgcgt gagcgagggt accgtgcgcg
     3601 gaatgcttcg agtccgccgg tccgtccggg acggccggac ttgacctcaa tggcccaaat
     3661 cagtttgccc gtgctgacga cgaagtctac ttcgtcattg ccttgccgcc agtagttcac
     3721 ggaatatctt tcgcgcggca ggtgattgag cagatgggcg cccgccgcat tttccacaag
     3781 ccgcccccac catgcgccgt cgtttttgga ctggtcaaag cttcgttgcg agaatgcgtt
     3841 gataagggcg ttgttccaca gaatcagttt ggggctgctg ccgcgcttgc gcggatgtcc
     3901 ggcggagtat tgcgaaaggc cggaggccag atacgcggtt tccagcagtt cgagataatg
     3961 ggccagggta gtggtgttgc cggcgtcctg cagttgaccg atcattttgt tataggaaaa
     4021 gatctgggcc ggatgggatg ccgccacgcc gaacagtgcc cgcagcaggg cgggtttgtt
     4081 cagcggatgc atctgcagca cgtcgcgcgc gatggctgtt tcgatgagcg agtcagagac
     4141 gtaacgggcc cattgggctt cattgtccgc gacggcggcg gcgccgggat agcctccaaa
     4201 atagagccat tgatcaaggt tccagccaaa ggcgcgcgaa cattcgggat aggtccaatg
     4261 catgcagcga tggagcagga accggccagc caggctttcg ctcatgcccc gatgaagcag
     4321 cagcgccgac gatccgagaa gaagcacgga cagcggaaac ttgtgacggc ggtcttcgtc
     4381 ccagagcctc ttgacggttt cgctccagcc gcgaaccttt tggatttcgt ccagaaccag
     4441 cagccggcga ggtccgggca atgcgcgagc ccgcatccac tgggtttcaa tccattccgg
     4501 cccgggggca ggaaaataat cggcggcttc aaacaccgtg ggccagccca atgtgttctg
     4561 gacatccatg gccgcagtgg ttttaccaac ctgccggggg cccgtcatga tttggatgag
     4621 ggccggcccg tcacgaaggg ccccgaccag ttctgacaca gcatgtattt ggtatttcat
     4681 gacggcataa caccataatg agaatcggtt tgtcaagatt actcaatgac tgagtaaaaa
     4741 tactcaatca ttgagtagct tggattaacg caaatttacg ttgatgcatt cccgcttcca
     4801 ctcattcatt cgcggttaaa aagtgccaaa aatggagaat tcatgaaaat atttcgcaca
     4861 aaggtacaga gacacaaaga ccccggaaag tattcatcat cttcatgaac ttcatgctct
     4921 tcatggtaaa acatcttttg aaaacctggc gccttcgcgg ctttgtgcgg ggaaaatgac
     4981 gataaatatg gaccgatccc cctatttgca cgataagcgc ttatcgtgca atagcgcgtc
     5041 gtaatgttgc agaacgtgca agacgcagaa aaatcgcaga gagaagcccg ttccgcgcac
     5101 acttttgctt ttctgtcttg caaaatcctg cccgccgcat tagtcttact ggtaagataa
     5161 cgattggagt gtgttatgat tgctgttgca acaacgaaaa tgtcatcaaa aggacaggtg
     5221 gtcatcccgg aggagctgcg aaaccgcatg aacctgtcgg ccggatcgca gtttgtggtc
     5281 gtgggggaag gggatgtcgt gattctgaag acgctgacag ttccctccat gaaacaattt
     5341 gaccggctga tccgtaaagc ccgcaggcag gcccggacag ccggcatgaa accccgggac
     5401 gttgagcggg cgatcgccgc ggttcggggt cgccagtgag ggtagtgctg gataccaaat
     5461 tgacgataag tttctggcct gtgcgatcgc cgcaaaagta aagtgcattt gcccgggcga
     5521 caaggccctt ctgaaaacat ccggatattg tggaatcaca gttatatcac cgcgtgattt
     5581 cattgagaaa catctgaact gaattcttcg attcgtgtgc atgcccgttc attcgcgggt
     5641 tatttatcat gaggaatgac gaggcttgcc agtgcatttt ttcgctttgg ccagtctggc
     5701 atgcattgcg ctaaaagatg ataaaagcct cttgaatgat tgtgatgctt aaggtggcag
     5761 agctcgtgca tgatgacata ttcgatgcaa tgcacagggg cttgaaccag attggtgttt
     5821 atggtaatac gaccttttct gctgcaactt ccccagcgcg tgtacatttt gcgcagagta
     5881 atctccgcag gaggaactcc gtgacgggcg gtcaccttgg tgcatttttc cagataccgc
     5941 cggaacgtat ctttggcccg ctcgcgatac cagttttcaa cagatcgttt tgctttgcag
     6001 gaactgcctt tatccgccac gctgaccagc aggaatcttc ctattaatct ggcgggtgca
     6061 ggctcgccct gtacaatctt cagtctgtac tgccggccca gataaagaag cgtctcgccg
     6121 ctcacatatt gtttcggcgc gggcaatggg tggaaagttt ttatacggtc cagtgtgcgg
     6181 acgatccagt gcgccttttt atgcaaaagt tcttcaatct gtgcatcccg tgcgcgaact
     6241 ggcgcggaaa ccgtcaccgc catatccgga gttacagcaa ttcgcacacg ttttcgcagg
     6301 ccgcggatca ggctataagg aatccgtcgg gatccgaact caagaatgcc ttcagttgtt
     6361 gaatcagctt tcattggcaa tcgcaatttc aatgcagcgg tcaatcagct tgtcctgcac
     6421 ttccagcggg aagctcagcc cgaactcttt cgcgacctca aaaaagatac tgtcaatttc
     6481 ccggcgcatc cggttgaggg cgtcgtgatt atccctccag tcgcgaacct tgtgccgatc
     6541 aatccgctta gccacttcga gggcgatggt tgccccgaca tcgggcttgt cggttgtata
     6601 ttgggcaatc tcttcattaa ccgttccaaa atagcgccgg gccatgtcat ttccgaccag
     6661 catttccggc acatcatctc cggtatgcgt ggccaccttt accgcaatat cttttatgcg
     6721 ttccagcgcc tctgccgcct gaagccgttt ttcatgcagt gcagccagaa cagtttcgag
     6781 cagttgtgaa aatttctgat agaacaccgg atccttttca aactcgtgtt cgatggtatg
     6841 ccgtgtggcg gaggcaatca tatctgcttt tgactctgcg ctttgacctt ctttttcaag
     6901 aacttgctcg cgctgttcgg tgttcagcag atcaatcggc tcgatgagtt gttccgcctc
     6961 cccggctccg acatgagtat ccagcagctt tttgattttc ggctcatact cggagaaatc
     7021 aacgcgttcc tgaaagcgga tggaggcgtc agcccgcaga ttccggaaga acttgaggtc
     7081 tttcttgtaa atgttgattt gcttctcggg ggtttgctcg agaaacgttg tggaagccag
     7141 cgcaagcccc atggcacggg caaatgcgct gaagcggtca tagaacttgc tgcgccgttc
     7201 ttcatcgcgc aggtgctcct gaaaaacttc gggatcggtg ctgccgcgga ctccggcgaa
     7261 aagctcccat aaatcggagt ggagctgggg cagcctggcg gcttgatcgg tgatgaacgt
     7321 aacggtttcc accaaatcag cgtggtcgta gtccgccagc ctgctgtaga aatcaatggc
     7381 ttcatcaaga ttttcaatca cgccgctgta gtccataatt agcccgtact ctttgccctc
     7441 ataaaggcgg ttcacgcggg caatggcctg aagcagggtg tgatctttca gcgaacgggc
     7501 cagatagaga accgtattgc acggagcgtc aaaaccggtc agcagcttat caaccacaat
     7561 gataatttca ggctcctccc cgtgtttgaa ggcattgatc aactgcttgt tgtatttttc
     7621 ttcagagcca tagcgatcca tcatcgcttt ccagaaaacc ttttcatctt cagaggcttc
     7681 tgttcctttg tcttcgccct cgcggacggc cggcccggaa acgagaacct cgcttttcac
     7741 catgccgaac tcatccagca gcttcttgta caggcgggcg gtttccttgt ccggggccac
     7801 aagctgaccc ttcatcccgg tgccctgata ggccatcgaa taatgcaggc tgacatccca
     7861 tgccaccatc cgcaccttct gcgcggcttt attaagctgt ttctcagatg aaaacttccg
     7921 tttcaaatca gccttctgct cttcattcag cggactggtc agccgctcaa accatccgtc
     7981 aatttcggct ttctggaccg gctgggggat atgacgcgcc tcatacacca gcggcaccac
     8041 cgctttatcg cgcaccgcgc gggaaatggt gtaggccggc ttaaacagct ctccgaactg
     8101 ggcaaaggtg tttttcttcg ggtctttggc cagcggagtc ccggtgaacc cgataaaaca
     8161 ggcgcccttg agcgccagct tcatccgggc atgatgttcg ctgtaattgc ttcgatggct
     8221 ttcatccacc agcacgaatg tgtttgggtc cgcttctttc agtccgctgc cggcggtagc
     8281 ggctgcaaat ttgtggatca gcgtcgtgat cacatgccgc tttccgtcgg tcagcatttc
     8341 gaccaggtgg gctccggtgt tcgcctgttc cggtttcagc ccgcaggcgg caaatgtgcc
     8401 ccaaatctga tcatcgagat ccacgcggtc ggtcaccaga ataatccggg ggctttcaat
     8461 cgctttggaa agggccagcg ccttggccag catcaccatc gtcagcgact tgccgctgcc
     8521 ctgagtatgc caaaccacgc cgccgttgcg cggctcgccg tttttcgcgg cgcagacccg
     8581 ctttaaaata tctttcacag tgaaaaactg ctgatagcgg gcaatctttt ttatgccatt
     8641 atcgaaaaga ataaactgat acatcagctc cagcagtcgg tcgggtcggc acattccata
     8701 caacgtgcga tcctgctcat agatcatccg gccctccttc agcagcttct caaacgcctg
     8761 ccggttcgcc ttgaacggac cgtcatccag cagtttattc agctggtcat cgggcagggt
     8821 ggttccgatc aaatcacgga tttccgcatc ggcgttttct tcccgccagc cgctccagaa
     8881 cttgcgcgga gtcccggttg ttccgtattc cgccttgtca cgggccagcg ccagcaggag
     8941 ctgactgtaa agaaaaagct gtggaatgcc gtctggcgcc tgataatcgg caagctgggc
     9001 ga
//
LOCUS       DAOQWR010000109         8989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8242_length_8989_cov_5.410231, whole genome shotgun sequence.
ACCESSION   DAOQWR010000109 DAOQWR010000000
VERSION     DAOQWR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8242_length_8989_cov_5.410231"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3290
                     /locus_tag="PKK36_08850"
     CDS             <1..3290
                     /locus_tag="PKK36_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR94700.1"
                     /translation="HYTFYTNGLQQDYVQTLRTDCGSVSFDYALADGSAMGTWDRSTL
                     TATYADGSTEVLYYNGGESYPAATYYTDRNGHRTKFAIGLGTAFPYQGRINSAEYADG
                     TQAEYEYNAALQVTNVSDEAGQSWGYDYNEQGRLTDVLTPSGYQLSFAYTNGGFDLAR
                     VTEAGSITLAAMTYTEKRDVATITNALNEAIRFGYDGFGRLTNAVDSLSLETVMEYGS
                     NGWVSEVKRDGITLSTFQQDDMGRTTNSVGPENIPVAFSFDNLNRLSSLAVSATEQPY
                     FWTYHTNSLLLEKQTDRSGRRTSLEYDELERVEKVRGPDSSYISFEYDSADNLSALLD
                     GKGSRTRFSYDARDRLTQKTWPGGGGLSVAYDARGLPVSHTGGRGFVTLMGYNGDGLL
                     TNVAYTATNTPGVRYSYNSRNLLISSADGWSTNSYSYDLLGRLTNMVEIGIAGTQRFF
                     YVYDPLGRMTNLLWQCGTNSINTSYAYDALGRVTNLVSDSGSYAYAYSNAGLQVSRLT
                     YPNGEYAASLYDSLGRLTNLAYSTGGSWTYAYDARDFVTRRVNPAGEVFTYIYDDAGR
                     LTDAIGLSGTNAISGYPLRYRYDKAGNRIRQTEGQKQRNLEYSEDNQLLSSTRTNILT
                     VRGYVNEAGSRVEAKSNSATNWIEGTVRFLSSTQSYFEIHGILTTNIYPSSNTVWVRA
                     FDVSNNVSTSVVHTAYSSTSKLEYEYDADGNLAEFPAPVQFYKTNVQYSWDAENRLTS
                     MVWRTKPQITNYFLVTTNKTFLYYDGAGRLRQISEYGTNSTPTGVVRFVWNGWTLAGE
                     LDGSNKLVRTYTCGVDLSGTVGGAAGIGGALGIRSYVSPVTNYYFRQDGKGNVTEVRR
                     YNGTTAASYGYYEFGSVRYQTNTYMQSIRWQTRMVHPQSGLYFWPQRIYDPATGRWCQ
                     RDRIGEAGGINIYQYVGGNPIGSFDPLGLDFIYIVDAEAVAGHGHAAAITGPVNGQWR
                     YDSFGVPRGFHRHGQYLFDTEQRAMEFARRHGYTRFARWQTRRDQDIAAQEQANLWHA
                     GLGQGYQMFREYKADDENCQSMVNDMARAAEIQEFIVLILQRGVNHPNVTMSRVLYGA
                     DESGVFE"
     gene            3287..3907
                     /locus_tag="PKK36_08855"
     CDS             3287..3907
                     /locus_tag="PKK36_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94701.1"
                     /translation="MNKKISFFQKLIVLLFCIIQGGCMTTGGKGIVQKEINPEEAMDI
                     VQKFYEILFSTTPPPDCPDIFEDSFGAQESRREEVEKMWSYIRENQNLFITDAYIDSK
                     YFTVHDYFEKAKKGIWYFNLPRGDLAHGGLLYVTVSSLLPIESGGVWKDVAIPVQKSG
                     KTGDVGIGFFGIRVNGLLLEYDGQYNREYNLIEALGFRPQKSDCQR"
     gene            complement(4352..4642)
                     /locus_tag="PKK36_08860"
     CDS             complement(4352..4642)
                     /locus_tag="PKK36_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HNR94702.1"
                     /translation="MKSSSSDWSGRVTRESHALALEKGVFTWDDPARIARSLMKSAEA
                     STARSAPPFRSAMSMLVFYINRAGKKLPPERKQTLEQAKEELRRLYDRKTRN"
     gene            4836..6584
                     /locus_tag="PKK36_08865"
     CDS             4836..6584
                     /locus_tag="PKK36_08865"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94703.1"
                     /translation="MRMLLITGMFCAVPVWGANNAADGDGAITVSPGQTLQGSVSNVF
                     TFSWRNTNVGKFYDGSQCTILFPAGWTPPQTNSPGCPGYIELTTVQKATAAISSVSGN
                     GPWTVTIDFTANQNGHGFDLKYAGGGNRVTAPAEAGSYTFVTKTKQNGGTLAEIAASP
                     VIVVQDPAGGNPGNRLETFDHFTYTGAAYASGTFLGQDGSVWTYARTRGDSSIEGRSP
                     TLEKSRGACIRSGTIPGGVGTLALKYRKAGQQRLNCSIFVNDTLAGTLSDGSGDIQTW
                     TSGVLNIEGDVVLLFTNSSSSGAVTLDDVSWTGYSGAPAAFTLKVQSACGFSEPAAGD
                     HVYLPGATITNTVQSPYMDGTTQYTCLGWTLSGHDPATGSTTRLVMTMTNDAVLTWHW
                     QTSFWLEAEADSYGEVSTGACWRAAGSTIRIEARAKPHYHFSKWDGDATGSATPLELV
                     MDAPKTLKARFAENLAAHNTPEWWLARHYPQADDFDEAALSDTDGDGYPAWQEWVAGT
                     DPTNGLCYLKFCPNHADELTFMARTGRQYSIFYKTSLQDSGWNVFTNSIPGKGQMVVV
                     TNTMPSAYFRLRVDRD"
     gene            complement(6627..7268)
                     /locus_tag="PKK36_08870"
     CDS             complement(6627..7268)
                     /locus_tag="PKK36_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94704.1"
                     /translation="MKTRIKEPNFLTVPDAARLCGVSRNTVYLWVRNEKIRAYQTPGR
                     TNLIRPCDLVEFMEQNGMFVAPELAALSREDRQLDAADPQTRPTEAGPCVLVVDDEPS
                     IRALYLRMLNNLAPLMQAETGYQALHLMTLHPNIRMVVLDLHMPGQHGLNTLREIKAQ
                     HPAVPVVVVTGYGADIPETMIQDGSIACVINKPVTSETLRNAVTRFLPGSPAA"
     gene            complement(7358..7431)
                     /locus_tag="PKK36_08875"
     tRNA            complement(7358..7431)
                     /locus_tag="PKK36_08875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7397..7399),aa:Gly,seq:tcc)
     gene            complement(7502..>8989)
                     /locus_tag="PKK36_08880"
     CDS             complement(7502..>8989)
                     /locus_tag="PKK36_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94705.1"
                     /translation="YRSKDGFSSPLTKGRSRTRVALAFEEQVLQGKPVYCNHMDSAAP
                     FCDDPVIHATGLRTYLGVPLRDSSGGLHGLLRLVDSEARDFQPIDVEMFTLAGLQVAD
                     WLKSERHGHITAELEAHLRQAQKMEAIGMLAGGIAHDFNNILSSILGFSSFLLSKVEP
                     ESQLYRPLSLIEKSAIHAADLTRQLLAFSQKKNLEQIPVSLNNVIRGVTGILEHSLRK
                     TICVETVLDKDLPMVLGDQGQLNQIIMNLCINASEAMEENGGTLRIESRFRPLTPREK
                     GVLLDVPGNLVCVSVSDTGRGMSEEVVEHIFDPFYTTKSKSGGTGLGLSIVYGLVAKH
                     GGDIRVESQKNKGSCFTVYLPPCRTAETQPGTSEEPPRSGGNETLLVIDDDEIILEMV
                     ASVLSSGGYRVLTASSGEAGVELLRKNRGAVDLVLLDMIMPDMDGEITFNALREIEPG
                     LAVLLTSGLARAEHCAKLIQRGAKGMISKPYTSARLLKDIQRVLG"
BASE COUNT         1970 a         2406 c         2686 g         1927 t
ORIGIN      
        1 cgcattacac tttttacacc aatggacttc agcaggatta cgtccagacg cttcgtacgg
       61 actgcggatc ggtatcattc gactatgcgc tcgcggacgg gtccgcaatg ggcacctggg
      121 accggagcac gctgaccgcg acctatgcgg acggatcaac cgaagtgctg tactataacg
      181 gcggagagtc ttatccagcc gcaacctatt acaccgaccg caacggccac cggacgaaat
      241 tcgcgattgg ccttggcaca gccttcccgt atcagggccg gatcaacagc gccgaatatg
      301 cggacggcac acaggcggaa tacgagtata acgccgcttt gcaggtcaca aacgtttccg
      361 acgaggccgg acagtcatgg ggctatgact acaacgaaca gggacgatta acggatgtgc
      421 tgactccctc cggttatcag ctgtcctttg cctacaccaa cggcggcttt gaccttgccc
      481 gcgtgacgga agccggaagc atcacgctgg ccgcgatgac ctacaccgaa aagcgcgatg
      541 tggcgacgat caccaatgcg ctcaatgagg ccattcgttt cgggtatgac ggattcggaa
      601 ggctgacgaa tgcggtggat tccctgagcc ttgaaacggt catggaatac ggatcaaacg
      661 gctgggtttc cgaggtaaaa cgggatggca ttacactgtc gactttccag caggatgata
      721 tgggccgcac cacgaactct gtcgggccgg aaaacatccc ggttgcgttc agctttgata
      781 atctcaaccg gctgtcctcg ctggcggttt ccgcaacgga acagccctat ttttggacct
      841 accacaccaa cagccttttg cttgaaaagc agacggaccg ctcgggccgc agaaccagcc
      901 ttgaatatga tgaactggaa cgcgtcgaaa aagtccgtgg gccggattcc tcgtatatat
      961 cgttcgaata cgattccgcc gacaaccttt ccgccctgct tgacgggaag ggcagccgta
     1021 cgcggttctc ttatgatgcc cgcgaccggc tgacgcagaa aacgtggccg ggcggcggcg
     1081 ggctgtccgt cgcgtatgat gcgcgggggc tcccggtttc ccacaccggc ggacgcggct
     1141 ttgtgacgct gatgggttac aacggcgacg ggcttttgac gaacgtcgcg tatacggcga
     1201 ccaacacgcc cggagtaaga tattcttaca actctcgaaa tctcctgatt tcgtcagcgg
     1261 acggctggtc cacgaacagc tacagctatg atctgctggg caggcttact aacatggtgg
     1321 aaatcggcat cgcgggcaca cagcggtttt tctatgtgta tgatccgctc ggacgcatga
     1381 ccaatctgct ctggcagtgc ggcacgaatt caatcaacac cagctatgct tatgacgcgc
     1441 tcgggcgcgt gacgaatctc gtcagcgaca gcggatctta tgcttatgcc tactccaacg
     1501 cgggcctgca ggtgtcgcgg ctgacctatc ccaacgggga atatgctgct tcgctgtatg
     1561 attcactggg ccggctgacg aatctggcct attccacggg cgggagctgg acgtacgcgt
     1621 atgatgcgcg ggatttcgtg acccggcgcg tcaatcccgc cggggaggtt ttcacgtaca
     1681 tctatgatga tgccggacgt ttgacggatg ccatcggtct ttcgggaacc aatgccatca
     1741 gcggctatcc gctgcgctac cggtacgaca aggcggggaa ccgcatccga cagacggagg
     1801 gacagaagca gcggaatctt gagtacagcg aagacaatca gcttctaagc tcaacgcgga
     1861 ccaacattct cacggtgcgg ggatatgtta acgaagcggg gagccgggtt gaagctaaga
     1921 gcaattcggc gacgaactgg attgagggta cggtgcgctt tctcagttcc acacagtcgt
     1981 attttgagat ccatggaatt ctgacgacga acatctatcc ttccagcaac acggtttggg
     2041 tgcgagcgtt tgatgtgtcc aacaatgtga gtacgtctgt tgtgcatacg gcctattcct
     2101 cgacaagcaa gctggaatat gaatacgacg cggacggcaa cctggcggag tttccggcgc
     2161 cggttcagtt ttacaagacc aatgtgcagt attcgtggga cgcggaaaac cggctgactt
     2221 caatggtatg gcgcaccaag ccgcagatca caaattattt cctggtgacc acgaataaga
     2281 catttctgta ctatgacgga gccggaaggc ttcgccagat cagcgaatac ggcacgaact
     2341 caacgcccac cggcgtggtg cgttttgtat ggaatggctg gacgctggcg ggtgagctcg
     2401 acggcagcaa caaactggtg cggacttaca cttgtggagt tgatctgtcc ggcacggttg
     2461 gcggagcggc gggcattggg ggcgctctgg gaatccgcag ttatgtttcc cctgtcacca
     2521 attactattt ccgtcaggac ggcaagggca atgtgaccga ggtccggaga tacaacggca
     2581 ccactgcggc cagttatgga tattatgaat ttggcagtgt tcgataccag acgaacacct
     2641 acatgcagag cattcgctgg caaacccgaa tggtgcatcc gcagagcggg ttgtatttct
     2701 ggcctcagcg catttatgat ccagcaactg gtcgttggtg tcaaagggac aggattggag
     2761 aggcgggcgg tatcaacatc tatcaatatg ttgggggtaa tcctattggt agtttcgatc
     2821 ccctcggttt agactttata tatattgtag atgcggaagc ggttgcggga catggtcatg
     2881 ccgcagctat aactggccct gttaatggac agtggcgcta tgattcgttt ggagttcctc
     2941 gaggctttca taggcacggc caatatctct ttgatacaga acagcgggcg atggagtttg
     3001 caaggcggca tggttatact cgctttgcac gttggcagac acgtcgggat caggatattg
     3061 ctgcgcaaga gcaggcaaat ctgtggcatg ctggattggg acagggctat caaatgtttc
     3121 gagaatacaa ggctgatgat gaaaactgtc aatcaatggt taatgatatg gccagagctg
     3181 cggaaattca ggagtttatt gttttaattt tacagcgggg agttaatcat cccaatgtga
     3241 cgatgtcgag agttctttat ggtgctgatg aatcaggagt attcgaatga ataagaaaat
     3301 atcattcttt caaaaactga tcgttttgtt gttttgcatt attcaggggg ggtgtatgac
     3361 aacaggcggc aagggtattg tacaaaaaga aattaatcca gaagaggcga tggatatagt
     3421 ccagaagttc tatgaaatac tctttagtac tacgcccccc ccagattgtc ccgacatctt
     3481 tgaggattct tttggcgctc aggagtcaag gcgtgaagag gttgaaaaga tgtggtctta
     3541 tataagggaa aatcagaacc tttttattac ggacgcctat atagatagca aatattttac
     3601 tgtgcacgat tattttgaga aggcaaaaaa gggaatatgg tatttcaatc ttccgcgagg
     3661 ggatttggcg catggagggc tgttatatgt gaccgtctcg tctcttctcc ccatagaatc
     3721 tggaggggtt tggaaagatg ttgctatccc tgttcagaaa tccgggaaga cgggcgatgt
     3781 aggaattgga ttttttggaa ttagagtcaa tgggttgttg cttgagtacg atggtcagta
     3841 taatagggaa tacaacctta tagaagcatt gggttttcga cctcaaaaaa gtgattgtca
     3901 gcgataaatt ataaggtcgc gcgggcaggg gccgccccgt ttcggggcgg tattcagaaa
     3961 aggcagaacc ccgccttcgg cgaggcggga acgaagcggg ccgccgcgat aagcgggccg
     4021 tgcaggcccg cgagagagct gagcgagcgc ttgccgcgcc ggagcttctg agcgaaggcg
     4081 aggccagcga gcgaagcctg aacggcgcgg cccagcgccg tccttgctcc gagccgcgtc
     4141 cagcggcgag gagtagaagc aaggcgagtc cgcgagcctt gcggggatag cgaggcgcgt
     4201 tagcgccgag ccggggagga cattgggcgt tccattccga gcggcgcgtc agcgccgcga
     4261 ggaagagggg ggcgcattga taaatgaaag ctgttctatt cttcacccat aacagaggtt
     4321 cgcacgagat tcaaaagaca ccgccagtca ttcagttgcg cgttttccgg tcgtacagcc
     4381 tgcgaagctc ttccttggcc tgctcaagcg tctgtttcct ctccggcggc agttttttcc
     4441 cggcgcggtt gatgtagaac accagcatgg acatggcgga gcggaacggc ggggcgctgc
     4501 gggctgtgct cgcctcggct gatttcatca gagagcgggc gatgcgcgcc ggatcgtccc
     4561 aggtgaaaac gcctttctcg agcgcaagcg cgtggctttc tcttgtgacc cgtccggacc
     4621 agtctgaact tgaagatttc atgtgacaga tggattcacc cgcaccgccg cctccgcctg
     4681 ccttcggcaa tccggtcctc catctaaaat tgaccaataa ttaataataa acattattga
     4741 caattaatac aaataatcca atagtgtgcg ctctttcttc acaaggagta gcaggtatta
     4801 aggattatac aaacagattt tcggcttccg catggatgag gatgctcctc attacgggca
     4861 tgttctgtgc ggtcccggtc tggggggcca acaatgccgc agacggggat ggtgccatta
     4921 cggtgtctcc cggccagact cttcagggca gcgtcagcaa tgttttcact ttcagctggc
     4981 gcaacaccaa tgtcggaaag ttctatgacg gatcacagtg caccattctt ttccctgcag
     5041 gctggacccc tccgcaaacc aacagccccg gttgtcccgg ctacatagag ctgaccacgg
     5101 tgcagaaggc aacggccgcc atttcatctg tttccggaaa cgggccatgg acagttacga
     5161 ttgatttcac cgccaaccag aacggccatg gcttcgatct gaaatatgcg ggcggcggca
     5221 accgtgttac ggcgccggcg gaagcgggca gttacacctt tgttacaaaa acaaagcaga
     5281 acggaggcac gctggccgaa atcgccgcaa gtccggtgat tgtggtgcag gacccggccg
     5341 gcggcaatcc cgggaacaga ctggagacgt tcgaccattt cacttatacc ggcgccgctt
     5401 atgccagtgg aaccttcctc gggcaggacg ggtcggtatg gacctatgcg cgcacgcggg
     5461 gtgattcgag catcgaagga cgctctccca cgctggaaaa gtcccggggc gcctgcatcc
     5521 ggtccggcac gattccggga ggggtaggca cactggcgtt gaaataccgg aaggccggac
     5581 agcagagact gaactgcagc atttttgtga acgacaccct tgcgggaacc ctgtccgacg
     5641 ggagcggcga catccagacc tggacgagcg gcgttttgaa tatcgagggt gatgtggtgc
     5701 tgctgttcac gaacagcagc agcagcggag ccgtcacgct ggatgatgtt tcctggaccg
     5761 gatactccgg ggcaccggcg gcgttcactc tcaaggtgca gtcggcctgc ggcttttccg
     5821 aaccggctgc cggtgatcat gtgtatcttc ccggcgccac catcacgaat accgttcagt
     5881 cgccgtacat ggacgggacc acgcaataca cctgcctggg ctggaccctg tcgggccatg
     5941 atccggccac gggatccacc acccggcttg tcatgaccat gaccaatgat gccgtgctga
     6001 cctggcactg gcagaccagt ttctggcttg aagcggaggc cgattcatac ggagaggtca
     6061 gcaccggcgc ctgctggcgt gcggccggct cgacgatccg gatagaggcc agagccaagc
     6121 cgcattatca cttttccaaa tgggatggcg acgcgacggg cagcgcgacg ccgctggaac
     6181 ttgtgatgga cgcccccaaa acgctcaagg cgcgctttgc cgaaaacctt gcggcgcaca
     6241 atacgcccga gtggtggctg gcgcgccatt atccgcaagc ggacgatttt gatgaggcgg
     6301 cgctctccga tacggatggt gacggctatc ccgcatggca ggagtgggtg gcgggaaccg
     6361 acccgacgaa cggcctgtgc tatctgaaat tctgtccgaa ccatgcggac gagctgacct
     6421 tcatggcgcg aacgggacgt caatacagca tcttctacaa gaccagtctg caggattcgg
     6481 gctggaacgt gtttacaaac agcataccgg gcaaagggca gatggttgtt gtcacgaaca
     6541 caatgcccag cgcctacttc cgccttcgag tggaccggga ctgaagcctg accggaagag
     6601 agccgcattc ggccgcacac ggccggtcag gcggccggcg agccgggaag gaagcgcgtg
     6661 acagcatttc gcagtgtttc agacgtgacc ggcttgttga tcacgcaggc aatgctgccg
     6721 tcctgaatca tggtttcggg aatatccgcg ccgtaaccag tcacgacgac caccggaaca
     6781 gcgggatgct gtgccttgat ctccctcagg gtgttcagtc cgtgctgtcc gggcatgtgc
     6841 aggtccagca ccaccatccg gatattcgga tgaagggtca tcagatgcag ggcttgatag
     6901 cctgtttcgg cctgcatcag gggcgcaaga ttgttcagca tgcgcaggta gagggcgcga
     6961 atcgagggct cgtcatccac aaccagcaca cagggcccgg cttcggtcgg ccttgtctgc
     7021 gggtcggcgg cgtccagctg ccggtcttcc cgggaaagcg ccgccagttc gggggcgacg
     7081 aacataccgt tctgttccat gaattccacg agatcacacg ggcggatcag attggttcgc
     7141 cccggcgtct gataggcccg gattttttcg ttccggaccc agaggtacac cgtgttgcgg
     7201 cttacgccgc aaaggcgggc ggcatccggc actgtcagga aattcggctc tttgatgcgc
     7261 gtcttcaatg atttttccgt tttctataca tagcacatgc cgatgatttt taaagtccat
     7321 aattgcggag agaagaagca cggttttgca ggaggggtgg agcgggtgaa cggaatcgaa
     7381 ccgtcacagc cagcttggaa ggctggagct ctaccattga gctacacccg cattgaaaac
     7441 cgcaccctac aatagccgct tgcggccgcg ggtcaagttt tcagcgacga aaaacgcgca
     7501 ttcagcccag gacgcgctgg atatccttca ggaggcgggc gctggtgtag ggcttgctga
     7561 tcatgccctt ggctccccgc tgaataagct tcgcgcagtg ctctgcccgt gcgaggccgc
     7621 tggtcaggag caccgcaagc ccgggctcga tttcgcggag ggcattgaag gttatttcgc
     7681 cgtccatgtc gggcataatc atgtccagca gaaccagatc aacggctccc cggtttttcc
     7741 ggagcagttc cacgccggcc tcgcccgaag aagcggtcag cacacggtag cctccggacg
     7801 agagcacgct ggcaaccatc tcaaggatga tttcgtcgtc atcaataacc agcagggttt
     7861 cgtttccgcc gctgcgcggg ggctcttcac ttgttccggg ctgggtttcg gccgtgcggc
     7921 agggcgggag atagaccgtg aaacagctgc ctttgttttt ctgtgattca acgcggatgt
     7981 cgccgccgtg tttggccacc agcccgtaca caatggaaag gcccaggccg gttcccccgg
     8041 atttgctctt ggtggtgtaa aacggatcga agatatgctc cacgacctct tcgctcatgc
     8101 cccggcccgt gtccgaaaca gacacgcaga ccagatttcc gggcacatcc agcagcactc
     8161 ctttttcgcg cggagtcagg ggccggaagc gggattcaat ccggagggtc ccgccgtttt
     8221 cctccatggc ctccgatgca ttgatgcaga ggttcatgat gatctggttg agctggccct
     8281 gatctcccaa gaccattgga agatccttgt ccaggacggt ttccacgcat atggtttttc
     8341 taagggagtg ttcgaggatg ccggtgacac cgcggatcac gttgttgagc gaaacgggaa
     8401 tctgctcaag atttttcttc tgcgagaacg ccaggagctg gcgcgtcagg tcagctgcat
     8461 gaatggcgga tttttcaatg aggctcaggg gccggtacaa ctgggattcc ggctccacct
     8521 tggacaggag gaaggaggag aacccgagaa tgctgctcag gatattgttg aaatcgtgcg
     8581 cgatgcctcc ggccagcatg ccgatggctt ccattttctg cgcctgccgc agatgggctt
     8641 ccagttccgc tgtgatgtgc ccgtggcgct cgctcttgag ccagtccgcc acctgcagcc
     8701 cggccagcgt gaacatctcg acatcgatgg gctgaaaatc gcgggcttcg gagtccacca
     8761 gccgcagcag cccgtgcaga ccgccggagg aatcgcgcag cggaacgccc agataggtcc
     8821 tgaggccggt ggcgtgaatc acggggtcat cgcagaacgg tgcggcggaa tccatgtggt
     8881 tgcaatacac cggcttgccc tgcagcacct gttcctcgaa cgccagcgcc acgcgggtcc
     8941 tgctccggcc ctttgtcaag ggggaagaga atccatcctt tgagcggta
//
LOCUS       DAOQWR010000110         8952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8274_length_8952_cov_5.721367, whole genome shotgun sequence.
ACCESSION   DAOQWR010000110 DAOQWR010000000
VERSION     DAOQWR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8274_length_8952_cov_5.721367"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="dapF"
                     /locus_tag="PKK36_08885"
     CDS             <1..588
                     /gene="dapF"
                     /locus_tag="PKK36_08885"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNR94706.1"
                     /translation="GARCVARLAAEIGAAPDNMTIETIAGELRAEARGDTVRLWMTAP
                     KDWRLDRTLQIEEASLGYSFVNSGVPHTVVPVDNLKTFNVKKYGALIRYHRDFAPAGT
                     NANFIAVTGPDSLAIRTYERGVEDETLACGTGMTAAGLIAGKLGLCRSPVKVTCASGD
                     TLEIGFTLTDDGARDVTLTGPAVHVFTGNLTYPLK"
     gene            672..2828
                     /locus_tag="PKK36_08890"
     CDS             672..2828
                     /locus_tag="PKK36_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR94707.1"
                     /translation="MIFLSVLLLSGPVSGSPPLGTEITPSGVAFRVWAPHARTVHVIG
                     DFNSWRATKQDQMERDPLTGIWEVHISRARAGRAYQFLINGELRRRDPYGRMVSPDES
                     KSVIYDPAAFDWSGDAPVTVNPDDLVIYEMHIGTFHDPNPADQSPGTFEDAVKRLDEI
                     AAMGINCVCVMPVHEFHGRHSWGYNPSDPFAVEQAYGGPDGFKTFVKACHQRGIAVHL
                     DIVHNHYGPQNLDLLQFDGTGRRDRGGIYFYDGSDTDALTPWGPRVKFDEPQVRRYIR
                     DNAQMWLDEYHVDGFRWDSTINIRAKNNGTEPLPEGAAMLEDINRFIRERWPRALSIA
                     EDSPGIGNFHGSWSYDFHHALRPVFRAANDADRSMDAVASVLNAPAGMPRVIYTDNHD
                     EAGKINGELRMASDADPARPESEKARRLCGLAAVIPLTAPGIPLIFMGNEMLMTGTFH
                     DDQPLNWRKRKIHEGLVTLHRDLISLRRNLNGVSGALKSRATHVPLIDNQQKTIVYWR
                     ENDEEPGDHAVIAINFSGTARETTIPFPDGGQWRTVLNTEWELYGGPVKKPGGSNFEL
                     KGTARKVKAPLGPYSARIFVRTGDRTPVRAPNPASPAARGDDRPLMTFYQSMGLAGTM
                     NGWNTTAWPMKLAADYVWEHIADFGGETQAEFKLAANGAWSINWGAPDDQVAHPPCTV
                     ALVEDGGNIRISGMRGGPWTFRFNEQTMMLEILGER"
     gene            2825..4276
                     /locus_tag="PKK36_08895"
     CDS             2825..4276
                     /locus_tag="PKK36_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNR94708.1"
                     /translation="MSAADIILFAVNARYAHTSFGLRCLLANLGPLRPRARMREYDLR
                     ITPEKIAADILAENPRIAAAGVYIWNHSRVRRTMEIIRERNPGIAVIAGGPELIAVQE
                     CPPWADHVITGEADIEFGRLCAGILNGEKSARLIRAEPADTANISLPYGEYTNEDIQN
                     RMIYVEASRGCPYRCEYCISSLDRKLRHFPQSAFFHDMEILLMRGARQFKFVDRAFNA
                     DIGSACDILDYFLARYTPGLRLHFEMMPDRFPRELRNRIMAFPPGALHLEIGIQTFNE
                     EAAARIMRKTDFSAAGENLAWLRRETGALIHADLIAGLPGETMESIAAGFDRLVSLNP
                     HELQFGILKKLPGAPIARHDTDWKMKYRSEPPYDILESASLSREQVEILKRFARFWDL
                     FHNRGNFPRFMQMLPGRAESPFTAFMKFSQWLEDRLGRISGIPQLELYAAALDYMNNS
                     GDARQALRDDYIAGGRRGHVPRFLKEAGTARRE"
     gene            4279..4932
                     /locus_tag="PKK36_08900"
     CDS             4279..4932
                     /locus_tag="PKK36_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94709.1"
                     /translation="MDAVWIVGPVILGITLAIIIISSVMETKRAAAMAAAAAALKLNF
                     QKKCGPDIIRELREFHLLQRGHSGRIMNLISGSAMNLDLRVFDYSYTISGGKSNTVVT
                     QTVISFTSPELELPDFELAPETFLHRIGEAFGARDIDFAEHPGFSKQYRLRGADEAAV
                     RALFGRNIREAFEARPGFSMEGRDHTLLFYRRGKRIRPEVLKDFMAEGFEILKLFRE"
     gene            5005..7104
                     /locus_tag="PKK36_08905"
     CDS             5005..7104
                     /locus_tag="PKK36_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94710.1"
                     /translation="MRIFGVSCGACVLFLSGLTAWSQVPPVVNYQGRIQVQGTNYTGA
                     GFFKFAIGTENMDTVFWSNDGTPAGEPAAAVQANLEKGLYCIGLGDNALAGMDALDPA
                     ILANTGLWLRAWFSDGVSGFEQLIPDQEFASAPYALVAGSVAGLSGFSVTNLDDVTDA
                     GSGQIITDAERGQIATNTSNISAMNVRLTQAEINVTALGSAVSGKVSRAGDTMTGPLF
                     INNHTTEGGIFIGSTGTTISVGTDANGSGHGLAVGRYANGAGYGAAHGYYADGSFIGA
                     AMGYSTKAGGYGAAVGSYANGTAYGAALGYNANAAFGGLALGASSHALNEGIALGQSA
                     TADGVERIAIGNRITNEVDHSTAVRGTLYLDGGTTVYYRASAGGGAWTDLLGGMATGT
                     PLYAFSEDDPVWEAEKTGYATGTPLYAFNELDPVWAAEKAGYATGTPLYVLSESDPVW
                     IAEKAGYATGTPLYVFTEADPFSVKISGDTMTGALNINVADAGGDLVIGTTGTAVSVG
                     RSANGTGNGAAVGRNANGAGYGAGVGFYADGSDNGAAIGYSAKGGWYGAAIGPLANGT
                     IYGAAVGRSANALGGSAVGFGANGAVFGAALGQDAKANHGGVAVGYLANGSSTNIAIG
                     MASAAAGLNRIAIGLNTTNTIDNSAAVRGSLYLDGGTGTFMRASAGTGNWTGFYTDGT
                     NLFFVNATGGVQQITSL"
     gene            complement(7111..8646)
                     /locus_tag="PKK36_08910"
     CDS             complement(7111..8646)
                     /locus_tag="PKK36_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNR94711.1"
                     /translation="MLAKIHSGAVYGVDAFIVEIEVNTGDGEPDVVTVGLPDAAVKES
                     KDRVWTALINSDYGTPLGRTTINLAPADVKKEGPRFDLPIALGVLVADGPLSADAVKD
                     YVVVGELALSGQVRPVKGVLPLAICARDAGFKGMLVPEANAEEAAVVEGLDVYPIRGL
                     RQAADFLQGMLPIEPCRVNRQKLFEINSVYDEDFRHVKAQEYAKRALEVAVSGGHNIL
                     AIGPPGTGKTMLARRVPSILPPMSLEEALETTKIHSIAGILAPHQALIACRPFRAPHH
                     TISDAGLLGGGSYPMPGEVSLAHNGVLFLDELPEFNRNVLEVMRQPLEDAFVTVSRVA
                     ASVTFPCRFMLVAAMNPCPCGYFGDTQRPCRCTPQQIQRYRNKISGPLLDRIDIHVEV
                     PTVRYEELSSLQEGESSSIIRQRVIRAREVQQERFRKSAHVHSNAAMTAGDMQKHCRM
                     NDDVHHLLKNAIQELNFSARAYNRILKVSRTIADLDGSELIEAPHISEAIQYRTLDRQ
                     LWA"
     gene            complement(8606..>8952)
                     /locus_tag="PKK36_08915"
     CDS             complement(8606..>8952)
                     /locus_tag="PKK36_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94712.1"
                     /translation="KELCRAVYFFDFLPDTSAQKQQKTAKNRKKTSKITKNGLKLINA
                     VNSTKLVLFTDFPRELHLNANEKALCCGHCGLKCPLFSRGGIVKFQNRCRTYRKDGHF
                     AGKNPFGRGVWR"
BASE COUNT         1842 a         2687 c         2719 g         1704 t
ORIGIN      
        1 ggcgcgcgct gcgtggcgcg gctcgccgcc gagatcggcg cggcgccgga caacatgacc
       61 atcgagacca tcgcgggcga actgcgggcc gaagcccggg gcgacaccgt ccggctctgg
      121 atgaccgcgc cgaaggactg gcggctcgac cggacactcc agattgaaga agcctccctc
      181 ggctattcct tcgtgaacag cggagtgccg cacacggtcg tgccggtgga caacctgaag
      241 accttcaatg tgaagaaata cggagcgctg atccgctatc accgggactt cgctccggca
      301 gggaccaatg ccaacttcat tgccgtgacg ggtccggatt cgctcgcgat ccgcacgtac
      361 gagcgcggag tggaggatga aacactggcc tgcggaacag gcatgacagc cgccgggctc
      421 atcgcgggga aactcggact ctgcaggagc cccgtaaagg tcacctgcgc cagcggcgac
      481 acactcgaga ttggtttcac cctcacggat gacggcgcaa gggatgtcac cctgacaggg
      541 ccggctgttc acgtgtttac agggaacctg acctatcctt tgaaatagtc cgcatatcgc
      601 gatcatggct ggcgtttttt gcctttaacg cgccgggccg gtctgctgta atcctgagca
      661 tgagagtctc cgtgattttc ctcagcgttc tgcttctgtc ggggccggtt tccgggtcgc
      721 cgcccctggg aacggaaatc acgccgagcg gagtggcctt ccgcgtctgg gcccctcacg
      781 cgcgcacggt gcacgtgatc ggcgatttca acagctggcg cgccacaaag caggatcaga
      841 tggagcgcga cccgctgacc gggatttggg aggtgcacat atcgcgggcg cgggccggac
      901 gggcctacca gttcctgatc aacggtgaac tccgccggcg ggacccgtac ggacgcatgg
      961 tttcgccgga tgaatcgaaa tccgtgatct atgatcccgc cgccttcgac tggtcggggg
     1021 acgcgcctgt gaccgtcaat ccggacgatc tcgtgattta cgaaatgcat atcggcactt
     1081 ttcacgatcc caatccggcc gatcagtcgc cgggaacatt cgaggatgcc gtcaagcggc
     1141 tggacgagat cgccgccatg gggatcaact gcgtatgcgt catgccggtg catgagtttc
     1201 acggccgcca cagctgggga tacaatccaa gcgatccttt cgccgtggag caggcctatg
     1261 gcggaccgga cgggttcaag accttcgtga aagcctgcca tcagaggggc attgccgtgc
     1321 acctggacat cgttcacaac cattacggcc cgcagaatct ggatctgctt caattcgacg
     1381 gcacgggccg ccgcgaccgg ggcgggatct atttctatga cggcagtgac accgacgccc
     1441 tgacgccgtg ggggccgcgc gtgaaattcg acgagccgca ggtgcggcgc tacatccgcg
     1501 acaacgcgca gatgtggctg gacgaatatc atgtggacgg attccgctgg gactccacca
     1561 tcaacatccg cgcgaagaac aacggcacag agccgcttcc cgagggcgcc gccatgctgg
     1621 aggacatcaa ccgcttcatc cgcgagcggt ggccgcgcgc gctttcgatc gcggaggact
     1681 cgccgggcat cggcaatttt cacgggtcct ggtcctatga tttccatcat gcgctgcggc
     1741 cggttttccg ggcggcgaac gatgcggacc gcagcatgga cgcggtcgcg tccgtgctga
     1801 acgcccccgc cggcatgccc cgcgtgatct atacggacaa ccacgacgag gcgggaaaga
     1861 tcaacggcga actgcgcatg gcctcggatg cggatcccgc ccggcccgaa agcgagaagg
     1921 cgcggcgcct ctgcggcctt gcggcggtga tccccctgac ggcgcccggc attccgctga
     1981 tcttcatggg caatgaaatg ctgatgaccg ggacgtttca cgatgaccag ccgctcaact
     2041 ggcgcaagcg gaaaatacac gaggggcttg tcacgcttca ccgcgacctg atttccctcc
     2101 gccgcaacct gaacggcgtc agcggcgcgc tgaagagccg cgccacccat gtcccgctga
     2161 tcgacaatca gcagaagacg attgtgtact ggcgggagaa cgacgaagag cccggagacc
     2221 atgccgtcat tgcgatcaat ttttcgggca cggcccggga aacgaccatc cccttcccgg
     2281 acggggggca gtggcggact gtactcaaca ccgaatggga actttacggc ggaccggtga
     2341 agaaacccgg cgggagcaac tttgaactga aagggacagc gcgcaaagtc aaagccccgc
     2401 ttggacccta cagcgcgcgc atttttgtgc ggaccggtga ccggacaccg gtccgcgcgc
     2461 ccaaccccgc gtctccggcc gcccgcgggg atgaccggcc gctcatgacg ttttatcagt
     2521 ccatgggcct ggccggaacc atgaacggct ggaacaccac cgcgtggccg atgaaactgg
     2581 ccgccgatta tgtatgggag cacattgcag atttcggagg agagacacag gcggaattca
     2641 agctggcggc caacggtgcc tggagcatca actggggcgc tccagacgac caggtcgcgc
     2701 atcctccctg caccgtggct cttgttgagg acggcggcaa catccgcatc tccggcatgc
     2761 ggggcggccc gtggactttt cgcttcaatg aacagaccat gatgctggaa atccttggag
     2821 agcggtgagc gcggcagaca tcattctttt cgccgtcaac gcgcgctacg cgcacacctc
     2881 gttcggcctg cgctgcctgc tggcgaacct gggaccgctg cggccgcgcg cacggatgcg
     2941 cgagtacgat ctgcgcataa caccggaaaa aatcgcggcc gatattctcg ccgagaatcc
     3001 ccgcatcgcg gccgccggag tctatatctg gaatcacagc cgcgtgcgcc gcacaatgga
     3061 gattatccgg gagcggaacc ccgggattgc cgtcatcgcg ggcggaccgg aactcattgc
     3121 cgtgcaggaa tgtcccccgt gggcggatca tgtcatcacc ggcgaggcgg atatcgagtt
     3181 tggtcgtctc tgcgcgggaa tcctgaacgg ggaaaagtct gcgcgcctga tccgcgcaga
     3241 gcctgccgat accgcaaata tttcgctccc ctacggggaa tacacgaatg aggacattca
     3301 gaaccggatg atctatgtgg aggcgtcccg cgggtgtccc tatcggtgcg agtactgcat
     3361 cagttcactc gaccgcaagc tgcggcactt tccccaatcg gcttttttcc acgacatgga
     3421 aatcctgctg atgcgcggcg cgcggcagtt caagttcgtg gaccgggcct tcaacgccga
     3481 tattgggagc gcatgtgaca ttctcgatta ttttctcgcc agatacactc ccggactccg
     3541 gcttcatttt gaaatgatgc cggaccggtt cccgcgggaa ctgagaaacc ggattatggc
     3601 gttcccgccg ggcgcgctgc atctggaaat cggcatacag acgttcaacg aagaggcggc
     3661 cgcaagaatc atgcggaaga ccgatttctc cgcggccgga gaaaacctgg cctggctgcg
     3721 ccgtgaaacc ggcgcgctca ttcacgcgga tctgattgcc gggcttccgg gtgaaaccat
     3781 ggaaagcatc gcggcggggt ttgaccggct ggtcagcctg aatccgcacg agctgcagtt
     3841 cggaattctc aagaaactgc ccggagcgcc gatcgcgagg cacgacacgg actggaagat
     3901 gaaataccgc agcgaaccgc cctatgatat tctcgaaagc gccagcctga gcagggagca
     3961 ggtggaaata ctcaaacgct tcgcgcgctt ctgggacttg ttccacaacc ggggcaattt
     4021 cccgcgtttc atgcagatgc ttccgggcag agcagaaagc cctttcacgg cgttcatgaa
     4081 gttcagccag tggctcgaag accggctggg acgcatttcc ggcatcccgc agcttgaact
     4141 ttatgccgcc gcactggatt atatgaacaa cagcggggac gcacggcagg cgctgcggga
     4201 cgactatatc gcgggcggac ggcgcggcca tgttccccgg tttctgaaag aggcgggcac
     4261 ggcccgcagg gagtagaaat ggatgctgtc tggattgtag ggcctgtcat cctgggcatc
     4321 acactggcta taatcattat atcctctgtg atggaaacga aacgcgccgc ggcgatggcc
     4381 gccgcggccg ctgcactgaa gctcaacttc cagaaaaaat gcgggccgga tatcattcgt
     4441 gaattgaggg aatttcatct cctccagcgc gggcactcgg gacgaattat gaacctgatc
     4501 agcggcagcg ccatgaatct tgacctgcgt gtttttgatt acagctacac catcagcggc
     4561 gggaaaagca acacggtggt gacccagacg gtcatcagct tcacatcgcc cgaactggaa
     4621 cttccggact ttgaacttgc gcccgaaacc ttccttcacc gcatcggaga agcctttggc
     4681 gcgcgggaca tcgactttgc agagcacccc ggcttctcaa aacagtatcg gctcaggggg
     4741 gcggatgaag ccgccgttcg ggccctgttc ggcaggaata tccgtgaagc ctttgaggcc
     4801 cggccgggat tttcaatgga gggtcgggac cacacccttt tgttctaccg ccggggaaag
     4861 cgaatcaggc ctgaagtcct gaaggatttc atggccgaag gctttgaaat tctgaaactg
     4921 ttcagggaat gaacgagcgg ttcccgcttg tttggcgagg ggtgctgcga taaaatacgg
     4981 gcctataaac tgtggagacc ggtcatgaga atattcgggg tgtcctgcgg cgcatgtgtt
     5041 ttgtttcttt cgggcctgac agcatggtcc caggtgccgc ctgttgttaa ttaccagggc
     5101 cgcatacagg tccagggcac taattacacc ggcgcgggct ttttcaagtt cgccatcggc
     5161 accgaaaaca tggacacggt gttctggagc aatgacggca ccccggccgg agaacccgcc
     5221 gccgccgtgc aggcaaatct cgaaaagggc ctttactgca taggtcttgg agacaacgct
     5281 cttgccggca tggacgcgct cgacccggcc atacttgcaa acaccggcct ctggcttcgc
     5341 gcatggttca gtgacggggt gtccggattc gagcagctca ttcccgatca ggaattcgcc
     5401 agcgcaccct atgcccttgt ggccggaagt gttgccggtc tctccgggtt cagcgtcaca
     5461 aaccttgacg atgtaaccga tgccgggagc ggacaaatca tcacagatgc cgagcgcgga
     5521 caaatcgcaa caaacacatc gaacatctcc gcaatgaatg tgcggctcac gcaggcggaa
     5581 atcaatgtga ccgctctcgg aagcgctgtc tccggcaagg tatcaagagc cggggacaca
     5641 atgacgggcc cccttttcat caacaaccac accactgaag gaggcatttt catcggcagc
     5701 acgggcacca ccatatccgt cggcacggat gcaaacggga gcggtcacgg cctggctgtc
     5761 ggccgctacg ccaacggggc cggctacgga gcagcgcatg gatattatgc cgatggttcc
     5821 tttatcgggg ccgccatggg ctattcaaca aaagcaggcg gctacggggc cgcagttggc
     5881 tcttatgcga acggcaccgc ctatggcgcc gcgctgggtt acaatgccaa tgccgccttc
     5941 gggggccttg cactcggcgc gtcctcccac gcgttgaatg aaggcatcgc gctggggcag
     6001 tcggcgacgg ccgacggggt cgagcgcatt gccatcggca accggatcac caacgaggta
     6061 gaccacagca cggccgtgcg cggcaccctt tatctggatg gcggcaccac ggtttactat
     6121 cgcgcttcgg cgggcggcgg cgcgtggact gatttgcttg gcggcatggc cacaggtacg
     6181 ccgctgtatg ccttcagcga agacgatccc gtctgggaag cggagaagac cggttacgcc
     6241 acaggcacgc cgctctacgc attcaacgaa ctggatcccg tctgggcggc cgagaaagcc
     6301 ggttatgcaa ccggaacccc gctgtatgtg ctcagcgagt cggatccggt atggattgcg
     6361 gagaaagccg gttacgccac aggcacgccg ctgtatgtgt ttaccgaggc ggatcctttt
     6421 tcggtcaaga tttccggtga caccatgacc ggcgcactca acatcaatgt ggcggatgcg
     6481 ggcggagatc tggtgatcgg caccacaggg accgccgtat ccgtcggacg cagcgccaac
     6541 ggaaccggca acggcgcggc ggtgggacgc aatgccaacg gcgccggcta cggagcgggt
     6601 gtgggctttt atgccgacgg atcggacaat ggggcagcga tcggatattc ggccaagggc
     6661 ggatggtacg gcgcggccat cgggccgctg gccaacggca ccatctacgg cgccgcggtc
     6721 ggccgttcgg ccaatgcgct tggcggttcg gccgtgggct tcggcgccaa cggcgcggtg
     6781 ttcggggcgg cgctgggcca ggatgcaaag gcaaaccacg gcggcgtggc cgtcggctac
     6841 ctggccaatg gatcatccac aaacattgca atcggcatgg cctccgcggc agcgggactg
     6901 aaccgcattg ccatcggctt gaacaccacc aacaccatcg acaacagtgc cgcggttcgg
     6961 gggtcgctct atctggacgg cgggacgggc accttcatgc gcgcatccgc cgggacaggg
     7021 aactggacag gcttctatac ggacgggacc aatctgttct ttgtcaacgc caccggaggc
     7081 gtccagcaaa tcaccagcct gtaacctccg ctaggcccag agctggcgat caagcgtgcg
     7141 gtactgaatc gcttcggaaa tatgcggggc ctcgatcagt tccgatccgt ccaggtcggc
     7201 gatggttcgc gaaaccttca gaatccggtt gtaggcccgg gcgctgaaat tcagctcctg
     7261 aatggcgttt ttcagcagat gatggacatc atcattcatg cggcagtgtt tctgcatgtc
     7321 ccctgcagtc atggccgcgt tgctgtgcac atgcgcggat ttcctgaaac gctcctgctg
     7381 cacttcgcgc gcgcggatga cgcgctggcg aattatcgaa gatgattcgc cctcctgcag
     7441 cgacgacagc tcttcataac gaaccgttgg aacttccacg tggatatcaa tgcggtccag
     7501 aagcgggccg gaaatcttgt tccggtagcg ctggatctgc tgcggcgtgc agcggcatgg
     7561 tctctgcgtg tctccaaaat atccgcaagg acaaggattc attgcagcca ccagcataaa
     7621 gcggcacgga aatgtgacac tggccgccac gcgggatact gttacaaacg catcctcgag
     7681 cggctgcctc ataacttcca gcacattgcg attgaattcc ggaagctcgt cgagaaataa
     7741 aactccgttg tgcgcaaggc tcacttcgcc cggcattgga tacgatccgc cgcccagcag
     7801 gcccgcgtcg gatatcgtgt gatgaggcgc gcgaaacgga cggcaggcga tcagcgcctg
     7861 atgcggggcc aggattcccg cgatactgtg aatctttgtt gtttcaagag cctcttcgag
     7921 actcatcggc gggagaatgg agggcacgcg ccgcgcgagc atcgttttcc cggttccggg
     7981 cggcccaatc gcaagaatat tatggccgcc gctcacggcc acttcgagcg cgcgctttgc
     8041 atactcctgc gccttcacat gacggaaatc ttcgtcgtat acactgttga tttcgaaaag
     8101 cttctgccgg ttgacgcgac agggctcaat cggaagcatg ccctgcagaa aatccgctgc
     8161 ctgacgcagt cccctgatgg gatatacatc cagcccttca acgaccgcgg cctcctccgc
     8221 attggcctcc ggcaccagca tccctttgaa accggcatcg cgggcgcaaa tggccaaagg
     8281 cagaacaccc ttcacagggc ggacctgtcc gctgagggca agctctccca cgacaacata
     8341 atccttcaca gcatcagccg acaaagggcc atccgctacc agcacaccca gcgcgatcgg
     8401 caaatcgaaa cgcgggcctt cttttttgac atcggcgggc gccagattga ttgtcgtgcg
     8461 gccgaggggt gttccataat cggagttgat cagtgcggtc cagacgcggt ccttgctttc
     8521 tttgacggcg gcatcgggaa ggccgacggt caccacatcg ggttcgccgt caccggtgtt
     8581 gacctcgatt tcgacaatga atgcatcaac gccatacacc gcgcccgaat ggatttttgc
     8641 cagcaaaatg accgtccttc ctgtacgttc gacaccggtt ttggaattta acaatcccgc
     8701 cgcgcgaaaa caaggggcat ttcaacccgc agtgtccgca gcaaagagcc ttctcattcg
     8761 cgtttaaatg cagttcccgc ggaaaatctg taaatagtac taatttagta ctatttacgg
     8821 cgtttatgag tttcagcccg tttttggtga tttttgacgt ttttttacga tttttggccg
     8881 ttttttgctg tttttgagca ctagtgtccg gtaaaaaatc aaaaaagtag acggctcgac
     8941 agagctcctt ca
//
LOCUS       DAOQWR010000111         8934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8287_length_8934_cov_5.204978, whole genome shotgun sequence.
ACCESSION   DAOQWR010000111 DAOQWR010000000
VERSION     DAOQWR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8287_length_8934_cov_5.204978"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PKK36_08920"
     CDS             <1..407
                     /locus_tag="PKK36_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR94713.1"
                     /translation="YRIRIGDQDIEGVGFALGVERVIMALEGEGIRPEQFIEPAQVWL
                     VSQGEAAFRENLLLAMTLRRRGISCQLDLTGRSMKAQMRMAGKSGAPQVIIRGDQEME
                     KGTFLLKDMKQGTQEEKDMPGLMLALSPVHPA"
     gene            512..2290
                     /gene="aspS"
                     /locus_tag="PKK36_08925"
     CDS             512..2290
                     /gene="aspS"
                     /locus_tag="PKK36_08925"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNR94714.1"
                     /translation="MTKMRTHTCGELSKNSVGQRVTLCGWADTVRDHGGVIFIDLRDR
                     YGMTQAIFDPRDSQEAWDIAQAVRGEYVVCVEGTVEMRPADMANRKLATGEIEVRCNV
                     IRVLNKSATPPFPLDEMQSQRVSEDLRLTYRYLDLRRKRMQDNLQMRHKVAMAIRTYL
                     DQAGFTEVETPILTKSTPEGARDYLVPSRVVPGTFFALPQAPQQYKQLLMIAGTDRYF
                     QVARCFRDEDLRADRQPEFTQIDMEMSFVTQDDIMAIVDGLVDAFMRASGHGAVQLPI
                     PRMTYREAMDRFGSDKPDLRFGMELTSLNDIFEETGFKVFGKVVADGGVVKAINAKGL
                     ANVPIRVVEDWTELAKEAGLAGLAFIRVQEDGTWKSPIVKFFSEAEQAQLRKRLNIEV
                     GDLILFAAHRAEIVNPFLGRLRLLAATMAGAIPEDKFVFTWVTEFPLFEVNDEGRLSS
                     MHHPFTAPFPEDLEKLQTAPLEMRAQAYDIVLNGTELGGGSIRIHDSETQSKMFEALK
                     FSREEISDRFGHLIRALSFGAPPHGGIALGFDRLMMLVTGSESIRDVIAFPKTQKAMD
                     IMMNAPAKVDAKQLRDVYLSLNLPET"
     gene            complement(2291..3283)
                     /locus_tag="PKK36_08930"
     CDS             complement(2291..3283)
                     /locus_tag="PKK36_08930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNR94715.1"
                     /translation="MYALLFEKPIVELEKKLEELRHVSADQALDVSGEIERIQKKIEQ
                     SRREIYENLTPWQKVQVSRHPMRPYPLDHIRRMTTGFLELHGDRDYADDRAIIGGFAM
                     LDGRSVMIIGTQKGRDTKSNLECNFGCPYPEGYRKALRLMKLAAKFKMPIITLVDTPG
                     AFPGIESEERHIAEAIAVNLREMFTLPVPFISVITGEGGSGGALGMAVCNRLLIMEHA
                     YYSVISPEGCAAILWKDRAYANQAADALRLTAKDLIQFKLADEVVPEPVGGAHTNYDA
                     AAEALKQKILKHLAALDKLSPQQLLEDRYRKFRAMGPFKEPEPESRRRFRRKKS"
     gene            complement(3302..4285)
                     /locus_tag="PKK36_08935"
     CDS             complement(3302..4285)
                     /locus_tag="PKK36_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNR94716.1"
                     /translation="MTDIYIRRNRENRGCFRLAVLAGIVAAGLLIWGIMTWRANRPPR
                     PPKPEQTEEQVFEERAAQAASPQAQPEAVARRETPPAPAPAGDRRGAVLLAEARKAQQ
                     DDDLLGARTRALALLAQSDNPGIISAAEQILNSVNIALATTPRPMPEKTEYTIKRGDT
                     ISGLAKKFKTTTQLIQKSNGIKGHVIRAGDLLRILSGEFAITVSKSQNDLVLTFNGQF
                     FKRYRVGTGKFGKTPTGKFVVSDKIVNPPWWRPDGKMVPYGDKENVLGTHWMALRSLD
                     DPGLRGYGIHGTWEPETIGTQASAGCIRLANDQVEELFSLVPEGTSVLITE"
     gene            complement(4299..5108)
                     /locus_tag="PKK36_08940"
     CDS             complement(4299..5108)
                     /locus_tag="PKK36_08940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR94717.1"
                     /translation="MDKDSFGGYSIIRDLAGGGMMHLYVARDAADNRVVIRTPKEEYR
                     RDRHIRKYFLKNAEILSQFRHPNIIRVLHFSKDGGIPFMLLEYVEAKNLRDLILYREA
                     LLTDNPLSLIRQLASAIYYLHSCNWLHLDIKPENILIRSDGLLTLIDFDLIMPRKNKP
                     QKVRQIPGTPSYVAPEVYQRHLVDERADIYALGTTCYEMLCHHKPFEGKTPEVSRQMQ
                     LDPRIRPAPMKQYNPAVPPRMESVIFKCLAKNPDERYPSMSLIIKDLESLI"
     gene            5235..6014
                     /locus_tag="PKK36_08945"
     CDS             5235..6014
                     /locus_tag="PKK36_08945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNR94718.1"
                     /translation="MYGDTHYELAAVRPTPVVRSLLIANVAVFAAQIFLDWLTRGAFT
                     REWFGLNIEGVLRHGRYWQFFTYMFLHGNLWHLLLNMLVLYFMGPETERGMGSSHFAA
                     LYLVSGILGGLGWFLISYPGSPCVGASGAIFGILGAFATLYPRRPVTLLVFFVLPVTM
                     EAWVLVTGLALIELASLVLGQGGHIAYAVHVGGVLVGAIYAAVVFRWGRDFRFRRPQM
                     RVLPGGRSSAEIDRILDKIAEHGMASLTRAERDALEKASRR"
     gene            complement(6017..6715)
                     /gene="rnc"
                     /locus_tag="PKK36_08950"
     CDS             complement(6017..6715)
                     /gene="rnc"
                     /locus_tag="PKK36_08950"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNR94719.1"
                     /translation="MKTAYKTLEKAIGYRFRHKDLLDTALTHRSFRHENGETNVDNER
                     LEFLGDAVLGSVAAAWLFNQFPEEQEGRLSMLKSAITSAKALACIGEKLEMGTYLQLG
                     RGEMLSGGNERCSNLANAVEAVLGAAYIDGGIKAVRKIFANLFLDELDRVLRQKFINP
                     KGMLQQYAQKYERCSPAYWILKEDGPPHAKNFTVEVRINGKLFGTGNGPNKKSAEICA
                     AQDALTRLPPKFLQ"
     gene            complement(6712..7599)
                     /gene="xerD"
                     /locus_tag="PKK36_08955"
     CDS             complement(6712..7599)
                     /gene="xerD"
                     /locus_tag="PKK36_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNR94720.1"
                     /translation="MHILAEQFLDFLRLERGLAENTLTAYRADLECFFSWLSARNIQS
                     VNAIKRRTVLDFLLHEKDRGLNTNTISRRLVCLKVFFRYLQQEGLVTGNITESMESPR
                     LWKILPDTLSVREVESLLASPDPEKAIGLRDKALLETLYGTGLRVSELAGLTLDDLHL
                     DESYIRCVGKGRKERIVPIGATAASWIQRYVSEARPSQIPDANARALFLTVRRQPFSR
                     KGIWKLIKKHVRQAGIGKNVTPHTLRHSFASHLLANGAPLRVIQEMLGHADISTTQIY
                     THVDPSRLKAIHSKYHPRS"
     gene            complement(7632..7961)
                     /locus_tag="PKK36_08960"
     CDS             complement(7632..7961)
                     /locus_tag="PKK36_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNR94721.1"
                     /translation="MRTTAGWIVMAGLALCCSGCWRQDIRTVGFSVPAMKSAECSKII
                     QDSLMAFEGILTAQPDLEQGVMYVTYDSKKLANVNVEHQIAALGFEVNTVPANADARA
                     KLPAACR"
     gene            8279..>8934
                     /locus_tag="PKK36_08965"
     CDS             8279..>8934
                     /locus_tag="PKK36_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNR94722.1"
                     /translation="MAPAGSYASLAAAVRSGADSVYFGIDRLNMRARAASPFTLDDLH
                     KIARICRRCRVRSYLALNTILYDEDLATVREICRAAKSAGLSAVIASDIAAIRIAREE
                     GLEVHVSVQANVSNMEAVRFYAQHAEVLVLARELSLEQIAAISRQIREEHLTGPSGRP
                     LQIELFVHGALCVAVSGKCYMSLAQYGTSANRGACYQTCRRKYLLSDAETGEQLAVEN
                     "
BASE COUNT         1689 a         2531 c         2775 g         1939 t
ORIGIN      
        1 gctaccggat caggatcggc gaccaggata ttgaaggcgt cggctttgcg cttggcgtcg
       61 agcgcgtgat catggcgctc gagggcgagg ggatcagacc ggagcagttc atcgaaccgg
      121 cgcaggtgtg gctggtttcg cagggcgaag cggcgttccg cgagaatctg ctgctggcaa
      181 tgaccctgcg acgccgcggc atcagctgcc agctggattt gaccggccgc agcatgaaag
      241 cccagatgcg gatggcgggg aaatccggcg cgccgcaggt catcatccgc ggcgaccagg
      301 aaatggaaaa gggaacgttc ctgctgaagg acatgaagca gggcacgcag gaggagaaag
      361 acatgccggg gctgatgctg gccctctcgc cggttcatcc ggcgtgaaaa agttccaacc
      421 attggaagtt tcttgaaaaa aagttccaat cattggaagt tttaccggca aaggttccaa
      481 tcattggaaa aacaggaaga agaacaaatt tatgacaaag atgcggacac atacatgcgg
      541 cgagttgtcg aagaacagcg tggggcagcg cgtgaccctg tgcggctggg ccgacacggt
      601 gcgcgatcac ggcggcgtga tcttcattga tctgcgcgac cgctacggga tgacgcaggc
      661 gatttttgat ccgcgcgact cgcaggaggc gtgggacatc gcgcaggccg tgcgcggcga
      721 gtacgttgtg tgcgtcgagg gcacggttga gatgcgtccg gccgacatgg ccaaccgcaa
      781 gctggccact ggagaaatcg aggtccgctg caacgtcatc cgtgtgctga acaagagcgc
      841 gacgcccccg ttcccgctgg acgagatgca gtcacagcgt gtgtcagagg atctgcgcct
      901 gacctaccgc tatctggatc tgcgccgcaa gcgcatgcag gacaacctgc agatgcgcca
      961 caaggtggcg atggccatcc gcacgtatct ggatcaggcg ggcttcacgg aggtggagac
     1021 gcccatcctg acaaagagca ctccggaggg cgcccgcgac tatctggtcc ccagccgcgt
     1081 ggtgccgggc acgttcttcg ccctgccgca ggcgccgcag cagtacaagc agctgctgat
     1141 gatcgccgga acggaccgct attttcaggt cgcgcgctgc ttccgcgacg aagaccttcg
     1201 cgccgaccgc cagccggagt tcacccagat tgacatggag atgtcctttg tcacgcagga
     1261 tgacatcatg gcgatcgtgg acgggctggt ggatgccttc atgcgcgcct ccggccacgg
     1321 cgccgttcag ctcccaatcc cgcgaatgac ctaccgcgag gcgatggacc gctttggaag
     1381 cgacaagccg gatctgcggt tcggcatgga actgacctcc ctgaacgata tttttgaaga
     1441 aaccggcttc aaagtgttcg gaaaggttgt tgctgacggc ggggtggtga aggcgatcaa
     1501 cgccaaaggg cttgccaatg tgccgattcg cgttgtggag gactggacag agctggccaa
     1561 agaggccggg cttgcgggtc tggctttcat ccgcgtgcag gaggacggga cgtggaaatc
     1621 tccgatcgtg aagttcttct ccgaggccga acaggcccag ttgaggaagc ggctgaacat
     1681 tgaggtgggg gatctgattc tttttgccgc gcacagggct gaaattgtca atccgttcct
     1741 cggccgtctg cggcttctgg ccgcaaccat ggccggggcc attcccgaag acaagttcgt
     1801 gttcacctgg gtgacggagt tcccgctgtt tgaagtcaat gacgaagggc ggctgtcctc
     1861 catgcaccat ccgttcacgg ccccgttccc cgaggatctg gagaagctgc agaccgcgcc
     1921 gctggagatg cgggcacagg cctatgacat cgtgctcaac ggcaccgaac tgggcggcgg
     1981 aagcattcgt atccatgatt ccgagacgca gagcaaaatg ttcgaggcgc tgaaattctc
     2041 ccgcgaggaa atcagcgacc gtttcggcca tttgatccgc gccctgtcgt tcggcgcgcc
     2101 gccgcatgga ggaatcgcgc tgggatttga ccgcctgatg atgctggtga cgggatccga
     2161 gtccatccgc gacgtgattg ccttccccaa gacgcagaag gcgatggaca ttatgatgaa
     2221 cgcgccggcc aaggtggatg cgaaacaact acgcgatgtc tatctgagcc tgaacctgcc
     2281 ggaaacctga tcagctcttt ttcctgcgaa accgccgccg gctttctggt tcgggctctt
     2341 tgaaaggtcc catggcccgg aatttccggt agcggtcctc cagcagctgc tggggcgaaa
     2401 gtttgtccag tgcggccaga tgcttcagga ttttctgttt cagggcttcc gcggcggcat
     2461 catagttcgt gtgggcgccg cctaccggtt ccgggacaac ttcatcggcc agtttgaact
     2521 gaatcaggtc tttggctgtc agccgcaggg catcggccgc ctgattggca taggcgcggt
     2581 ccttccacag gatggcagcg cagccttcgg gggaaatgac agagtaatag gcgtgttcca
     2641 tgatcagcag ccggttgcac acggccatgc cgagcgcgcc tccgctgccg ccttccccgg
     2701 tgatcaccga aatgaacggg accggcagag tgaacatttc gcgcagattg accgcaatgg
     2761 cctcggcgat gtgccgctcc tctgactcga tgcccgggaa ggcccccggg gtgtccacca
     2821 gcgtgatgat cggcatcttg aacttggccg ccagtttcat caggcgcagc gccttgcgat
     2881 agccttccgg ataggggcag ccgaagttgc attcaagatt gctcttggtg tcgcggccct
     2941 tctgggtccc gatgatcatg acgctccgtc cgtccagcat ggcgaagccg ccgatgatgg
     3001 cgcggtcgtc cgcatagtcg cggtccccgt gcagctcgag aaacccggtg gtcatccggc
     3061 ggatgtgatc cagcggatag ggtcgcatgg gatggcgcga gacctgcacc ttctgccatg
     3121 gggtcaggtt ttcgtatatc tcgcggcgcg actgctcgat tttcttctgg attcgctcga
     3181 tttctccgga cacatccagc gcctggtcgg cgctgacatg tcgcagctct tcgagcttct
     3241 tttccagttc cacaatcggt ttttcaaaaa gcagcgcgta catggcgttt ctccagacgg
     3301 attattccgt gatcagaacg gaagtgccct cgggcaccag cgaaaacagc tcttcaacct
     3361 gatcgttggc cagacggatg cagccggcgc tggcctgcgt gccgatggtt tcaggttccc
     3421 atgtgccgtg gatgccgtag cctcgcaggc ccgggtcgtc cagcgaccga agcgccatcc
     3481 agtgtgtgcc cagcacattt tccttgtcgc cgtagggaac catcttgccg tcaggccgcc
     3541 accagggcgg gttcacgatt ttgtctgaaa caacgaattt ccccgtcgga gttttcccga
     3601 atttccctgt tccgacgcga taacgcttga aaaactggcc gttgaaggtc aggacaaggt
     3661 cgttttgcga cttgctgacc gtgatcgcaa actctccgga gagaatgcgc agcagatcac
     3721 cggcgcgaat gacgtgcccc ttgatgccgt tgcttttctg gatcagctgg gttgtggttt
     3781 tgaatttttt ggccaatccg ctaatggtgt ccccgcgctt gatggtgtac tcggtctttt
     3841 cgggcatggg gcggggcgta gtggccagcg cgatgttgac ggagttgagt atctgctccg
     3901 ccgcggaaat gatgccggga ttgtcggact gcgccagcag agcgagcgcc cgtgtgcgcg
     3961 ccccgagaag atcgtcatcc tgctgcgcct tgcgggcctc ggccagcagt acggcgccgc
     4021 ggcggtcgcc cgccggggcg ggggcggggg gcgtttcgcg ccgggcaaca gcctcgggct
     4081 gagcctgcgg agacgctgcc tgcgccgcgc gctcttcaaa gacctgctct tcggtctgtt
     4141 ccggtttcgg gggcctgggc ggacggttgg cgcgccaggt catgatgccc cagatcagca
     4201 gaccggccgc cactatgccg gccagcacag ccaggcggaa gcagccgcgg ttctctcgat
     4261 ttcggcggat gtagatatct gtcatagtcc cggttcactc agatgagtga ctccaaatcc
     4321 ttaataatta aactcatgga aggatatctt tcgtcagggt tcttcgcaag gcatttgaag
     4381 atgacagact ccatgcgggg ggggaccgcc gggttgtact gcttcatcgg tgccggacgg
     4441 atgcgagggt cgagctgcat ctggcggctg acttcggggg tctttccctc gaagggctta
     4501 tggtgacaga gcatttcata gcacgtggtg cccaaagcat agatgtccgc gcgctcgtcc
     4561 acgaggtgac gctgataaac ctctggcgcc acataggacg gtgtgcccgg aatctgcctg
     4621 actttctgcg gtttgttctt gcggggcatg atcaggtcga aatcaatcag cgtcagcagc
     4681 ccgtcgctgc ggatcaggat gttttccggc ttgatgtcca aatggagcca gttgcagctg
     4741 tgaaggtaat agatggcgga cgcaagctga cggatcaggg aaaggggatt atccgtcagc
     4801 agcgcttcgc ggtacagaat aaggtctcgc agattcttgg cctccacgta ttccagcagc
     4861 atgaagggaa tcccgccgtc ttttgagaag tgaagcaccc ggatgatatt cggatgccgg
     4921 aactgcgaga gaatttcggc atttttcaga aagtatttgc ggatatgcct gtcccggcgg
     4981 tactcctctt tcggagtgcg gatgaccacc cggttgtccg ccgcatcgcg cgcgacatac
     5041 agatgcatca tgcctccgcc ggcaaggtcg cggatgattg aataaccgcc gaaagaatct
     5101 ttatccatgg ccctaggcta atgaatgaag gggcggatgc aaggctaaat gagggcgtgt
     5161 cggaaaaagc gccggcaggg caaataattg aaaaagccgg cttcagaaag gcgcgaatgt
     5221 gataacatcc ggatatgtat ggagataccc attatgagct ggccgccgtg cgtcccactc
     5281 cggtggtgcg ctcgctgctg attgccaatg tggctgtgtt tgcggcgcag attttcctgg
     5341 actggctgac ccgcggagca ttcacccgtg agtggtttgg cctgaatatc gagggagtgc
     5401 tccggcatgg ccgttactgg cagttcttca cttatatgtt cctgcacgga aatctctggc
     5461 atctgctgct gaacatgctg gtgctctatt tcatggggcc ggagaccgag cggggcatgg
     5521 ggtcgtcgca cttcgccgca ctttatctgg tcagcggcat cctgggcggg ctgggctggt
     5581 ttctgatcag ttatccgggg tctccgtgcg tgggcgcatc gggcgccatt ttcggcattc
     5641 tcggggcgtt tgccacgctc tatccgcgtc ggccggtgac gctgctggtc tttttcgtgc
     5701 tgccggtcac tatggaagcc tgggtgctgg tcacggggct tgcgctgatt gagctggcat
     5761 cactggtgct ggggcagggc gggcatattg cctatgccgt gcatgttggc ggggtgctgg
     5821 tgggcgccat ctatgcggcc gtggttttcc gctggggacg cgatttccgt tttcgccggc
     5881 cgcagatgcg ggtgctcccg ggcggtcgtt cctctgcaga gattgaccgg atactcgaca
     5941 aaatcgccga acacggcatg gccagcctga cacgggccga acgcgatgcg ctggaaaagg
     6001 ccagccggcg ctgactctac tggaggaatt tcggcgggag ccgggtgagc gcatcctggg
     6061 cggcgcagat ttcggcggat tttttgttcg gcccgtttcc tgtcccaaac agcttgccgt
     6121 tgatgcgcac ttcaacggtg aaattcttgg catggggcgg tccgtcctct ttcagaatcc
     6181 agtacgccgg actgcagcgc tcatatttct gggcgtactg ctggagcatg ccctttggat
     6241 tgatgaactt ctgtctcagg acccggtcca gctcatccag aaacagattg gcaaagattt
     6301 tgcgaacggc cttaatgccg ccgtcaatat acgccgcccc caggaccgcc tcgacggcat
     6361 tcgccaggtt ggagcatcgc tcgtttccgc cagacagcat ttccccgcga cccagctgca
     6421 ggtaggttcc catctccagc ttttcgccga tgcaggcaag ggccttggcg ctggtgatcg
     6481 cgcttttgag catggaaagc cgcccttcct gctcctccgg aaactgattg aagagccagg
     6541 ccgccgccac ggatcccaga acggcatctc caaggaactc cagccgttcg ttgtccacat
     6601 tggtctcgcc gttctcgtgc cggaaagagc ggtgcgtgag cgccgtgtcc agcaggtcct
     6661 tgtggcggaa gcggtatcca atggcttttt caagtgtttt gtatgctgtc ttcatgatcg
     6721 cggatgatat ttgctgtgga tggctttcag tcgggacggg tcaacatgag tataaatctg
     6781 tgtggtggat atatccgcgt ggcccagcat ttcctggatc acgcgcaggg gcgccccgtt
     6841 ggccagcaga tgactggcga aggaatgccg cagggtgtgc ggcgtcacat ttttccctat
     6901 gccggcctgc cgcacatgtt tcttgatgag tttccagatg cccttccggc tgaacggctg
     6961 ccggcggacc gtcaggaaaa gggcccgggc attggcatcc gggatctgag acggccgcgc
     7021 ttcgctcaca tagcgctgta tccatgaggc ggcggtggcc ccgatgggaa caatgcgttc
     7081 cttgcggccc ttgccgacac agcggatgta tgactcgtcc aggtgcagat catccagggt
     7141 cagcccggcc agttccgaca cccgcaggcc cgttccatac agcgtttcca gcagggcctt
     7201 gtcccgcagt ccgatcgctt tttccggatc agggctcgcc agcaggcttt cgacttcgcg
     7261 aacactcaaa gtgtccggca ggattttcca gagccggggc gattccatgc tttccgtgat
     7321 gttcccggtg accagtccct cctgctgcag atagcggaaa aacaccttca ggcagaccag
     7381 ccggcgggaa atggtgttgg tgttgagtcc gcggtccttc tcgtgcagca gaaaatcaag
     7441 aacggttctt cttttgatgg cgttgacgga ctgaatgttc cgggctgaca gccaggaaaa
     7501 gaaacattcc agatcggccc gataggcggt cagtgtgttt tccgcgagtc cccgctccag
     7561 tctcaaaaag tccagaaact gttctgcaag tatgtgcatg agcgctctcc tggccccgcg
     7621 gggtttgggc gtcagcggca ggcggcgggc agtttggccc gggcatccgc gttggcggga
     7681 acagtgttca cctcgaatcc gagtgccgcg atctggtgct ccacattcac gttcgcgagt
     7741 ttcttcgaat cataagtgac atacatcacg ccctgttcca ggtccggctg cgctgtgaga
     7801 atgccttcaa acgccatcag gctgtcctga atgatcttgc tgcattcggc gctcttcatg
     7861 gccggcacgc tgaagcccac cgtccggata tcctgacgcc agcatccgct gcagcagagt
     7921 gccagccccg ccatcacgat ccacccggct gttgttctca taaacggtcc ctttctgatt
     7981 cgaacaaacc taccggaacc cggacgccgg gtcaatgcct cgcagacccc ggaaaataac
     8041 ttaaaataaa attaaacaaa aaaaggggcg ggggtatcta tattgctgtg aagataaaaa
     8101 tggggggttg gaagcgtttc ctccggggct cctcacccca acctccttgg cgcttacgac
     8161 cccccattat cttttcgccc cctcgcgggg gcttttcttt ttgggcttta tctgttggcg
     8221 cggggacggc attccggttt acattttcag cgtgaagaac agcagaccag tagaactgat
     8281 ggcgccggcg ggatcgtatg catcccttgc ggcggccgtg cggtccggcg ccgattccgt
     8341 ttatttcgga atagaccgcc tgaatatgcg cgcgcgcgcg gcgagtccgt tcacgctcga
     8401 tgatctccat aaaatcgcgc gcatctgccg gcgctgccgt gttcgcagtt atctggccct
     8461 gaacaccatt ctgtatgatg aggatctggc caccgtgcgt gaaatctgcc gcgcggcaaa
     8521 atctgccggc ctgtccgctg ttattgcttc cgatattgcc gccatacgga ttgcgagaga
     8581 ggagggactg gaggtccatg tttctgttca agccaacgtc tcaaacatgg aggcggtccg
     8641 gttttatgcg cagcacgcgg aagtgctggt gctggcccgc gaactttcgc tggagcagat
     8701 tgcggccatc agccggcaga tcagggaaga gcatttgaca ggtccttccg gccgcccgct
     8761 gcagattgaa ctttttgttc acggggcact ttgcgtcgct gtttccggga agtgctatat
     8821 gagccttgct cagtacggca catcggcaaa ccgcggagcc tgttatcaga cctgccgcag
     8881 gaaatatctg ctcagcgacg cagagacagg ggaacagctt gctgttgaaa atca
//
LOCUS       DAOQWR010000112         8920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8299_length_8920_cov_4.454033, whole genome shotgun sequence.
ACCESSION   DAOQWR010000112 DAOQWR010000000
VERSION     DAOQWR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8299_length_8920_cov_4.454033"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PKK36_08970"
     CDS             <1..1010
                     /locus_tag="PKK36_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94723.1"
                     /translation="TVTTTNQVPLLTSAEGEEAFEDQVREEQVITPLEEVQDEILAKL
                     IREAALNKALDEATDFVVALAPNRKGQSVPFETQAKKAKRDVLTLESFTLDEELTELK
                     VGPDFNEIAFSLRPNPDEYFSDAIAGEEAVYVLALEERIPARVPEFAEVEDQVREIAT
                     AVAKKEALDELVQSVRESAEQAVREGNTFASVLESSGLVMQTATNVSAMTGIEDEQFA
                     DYSDLIMRAAITSNPGEIPEAIETEEGFILCHIASRTPGDASLFETYRANLVTALRQD
                     RLGVNFGAFQEWLLNEADFQPRSFGEWDDEEPEEEDEEYADEELYKEEIPVEADETAA
                     E"
     gene            complement(1012..2208)
                     /locus_tag="PKK36_08975"
     CDS             complement(1012..2208)
                     /locus_tag="PKK36_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR94724.1"
                     /translation="MPYITKGLLKDFYLPDYADGSTVNLLASIIRSRGGKSPHKNLKG
                     LDPRRLREARNVIYLVVDGLGVSQLERFLSMGKGKQFFAKHPWTTISSVFPATTAAAV
                     TTFATGASPAEHAVLSWYLNLHDLGVVTMILPAITRTGSPLAREEFDLHAYLDLPSYI
                     DSVKGRRGLISYEDIPQSRYSRAGTRWTDRMAARTLQGLERQIMAFVRKRGRKMAYAY
                     WPEYDSNCHEHGVTHRKTDRHLRQIDESLARLVRKLQGTDTRLFVLADHGLIDAPAAQ
                     RIDLGAVPGFYGCLATITSGDARSVSCFVRPGKVKAFKEIVDRKLGHACVCTPGEVLL
                     AMNAFGPGKPHKALANRAGDFFLMARPGYAFTATLPGWEPRFHVAYHGGMSADEVLVP
                     LYSVQC"
     gene            complement(2280..3416)
                     /locus_tag="PKK36_08980"
     CDS             complement(2280..3416)
                     /locus_tag="PKK36_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94725.1"
                     /translation="MKRMDVSRIVCIADVRLTERLEAEADRIGLRETWSTRGKQVALK
                     ARSVWQPFGRRLPMAESPSDIIRFYVPRQHERAAMIRLAAAADLYLPGRGSVYAEDVA
                     LIGDGVQPWDEEKLCADQPSWVVENRMEPAPYDMIYCIVQRGRGGELARTMLEMGLCV
                     PIVSFGEGMGMRDRLGLLRITIPQEKEILLFVVPPGDSDFVMDVAVRKANLHEPGNGF
                     LCRVPVRALAVNSRMHLDRRKHVATMEQVISTLDQLRGSTDWRRMSAAARHHRGAEPE
                     GLVRFTLICEEGTSSAHVRAALDAGAGGATLSLLSRRVPAPHEGGGEVAMASRARECC
                     DLVIPRALKERIEEAVTRTGLFAESSGGFIETGIVYDAITAAGR"
     gene            complement(3413..5389)
                     /locus_tag="PKK36_08985"
     CDS             complement(3413..5389)
                     /locus_tag="PKK36_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HNR94726.1"
                     /translation="MPESVQSTSVMLQIPLRRKLSLIWTYARERLMRQVHAVAFITGY
                     LALFQLLVLRAPIADFLKIAAGILAVVAGLAFFMEGLFLAIMPLGERCGLRMPARAGL
                     GLLVAFAVILGITATYAEPAIGFLKAQGSSTLPWRAPLLYYLLNAGAPLTVGAVAVGV
                     GFAVVLGVFRSLRSWSFKPFLYLTIPVLLALSYWVSRDPRTAAIIGLAWDTGGVTTGP
                     VTVPLVIALGIGVSRIAGRGDEPSSGLGVVTLASALPVIMVLLLGAVLAPRFPMPGGP
                     DEFFAPERHNQSVRVAGSEEAFRELAANNLSPEKVKTRFGTESKEKPSDAGPRRHVPR
                     KMMRAHAVNALKAIVPLAAVLLLALLLIVRERLPHNDEVSLGLVFSLVGMLLFSYGMD
                     RGLSSLGNQAGRSLPRAWEATERPDKAVTYSGINENLLVRAVRPNGSVAEYLPLADKG
                     GPQFVPFIRERYDADRAEYRWIPEQGPVAGDEDFWGYALVLLFVFVMGLGATIAEPSL
                     NALGVTLEELTTGTYKRSFLIVTVALGVGAGMAMGFCRILFAWPMIPLLAGCYLVALA
                     LTCISTEEMSAIAWDCAGVTTGPITVPLVIAAGLGIGQRAGAAEAFGVVTMASVFPII
                     AVLLSGFLIAARRNALDLENLQMDAAPAEREAKP"
     gene            complement(5495..6913)
                     /locus_tag="PKK36_08990"
     CDS             complement(5495..6913)
                     /locus_tag="PKK36_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNR94727.1"
                     /translation="MHGGDDHGGELTLRQMMVRMALLLFISGTTGLCGVRMGLNFSQA
                     VATSVFLAIILGTLFFWNFRLAIAFLGIGVLIFTRSLDIDHFVKEASLPVILFLVGMM
                     IVVGALRDLGFFTWIVQSIISIPKITGRKFMAITAVASALLACAVDEVTSIIFISTLI
                     FQVCNRLKLNPAPYLMICVLCTNIGSSGTMMGNPVGIYIGTKAHLSFADFIRWAFPVM
                     LLALGSCVAVTMFWYRRDLDEFDVRLKDRMDRDLSLVPKVKVPYGRGLAVLLLTLVFI
                     ALHHTLEEALHLDPNSILLVAPLACAGVIMIFKRDRARHYVEAEVDWWTLLFFMLLFA
                     IAGTLAYTGVTRLMADGFTRRFGTDLNVLIPVIMATTALGSAFVDNVIFVAAFAPIIE
                     ALGESVKIMPLWWALLFGACFGGNITLIGSTANIVALGMLEKECRIQISFFRWLKIGA
                     LSSLVACLIAWGMLALLAPLMP"
     gene            complement(6963..7868)
                     /locus_tag="PKK36_08995"
     CDS             complement(6963..7868)
                     /locus_tag="PKK36_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNR94728.1"
                     /translation="MDTQLRDCLSPENILFEVAATTRDEVLTLLVNHLESTLDGFDPQ
                     EALHALKEREAVLPTVIAPGVALPHARLDNMDKTLIAVATTRGGIVFDADKDPVNLIL
                     LVLTPKNDPSSYLRVLALLSGALKDLKIREFIKEASAETVSALFRSGDRGQGEYLTAA
                     DIMNARPLTLKEGDTLRAAITALCSRRLLDISVVDDQGDLRGVICMEDLLSQSLPQHL
                     LWMEDLSPILRFEPFAEMMKKDNETKLADFMHEDYVSVSPDTPAVQLAKIFIMKHTRQ
                     IQVLEGRRLAGVVSLAGFTTKLFWA"
     gene            complement(8108..8184)
                     /locus_tag="PKK36_09000"
     tRNA            complement(8108..8184)
                     /locus_tag="PKK36_09000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8148..8150),aa:Arg,seq:tct)
     gene            8304..8909
                     /locus_tag="PKK36_09005"
     CDS             8304..8909
                     /locus_tag="PKK36_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94729.1"
                     /translation="MPSQSSRERGSSPAGIFIKLILLLIGAAVLLALAADVSSWIDKQ
                     KTDPVPPPETEIQSIILQSHPRVFRDVNGLFTMNAPDGWEITAWPDSTPYNVTFQSRN
                     GPSISITATRVEYNSLVTLRKRIEAVQRDYGLNMNIAQTNWNGREIIQRTTVLHASKV
                     RTLDFVENYVGHNIQISIPHSLYDRYLPVLLDVAESYKPLK"
BASE COUNT         1972 a         2790 c         2593 g         1565 t
ORIGIN      
        1 agaccgtgac caccaccaac caggtgcccc tgctcacctc ggccgagggc gaggaggctt
       61 ttgaggacca ggtccgcgag gaacaggtca tcaccccgct ggaggaagtg caggatgaga
      121 ttctcgcaaa gctgatccgc gaggcggcgc tcaacaaggc gcttgatgaa gccaccgatt
      181 tcgtcgtagc cctcgcgccc aaccggaagg gccagtccgt tccgtttgaa acccaggcca
      241 aaaaggcgaa aagggacgtg ctgacgctgg agtccttcac cctggacgag gaactgacgg
      301 agctcaaggt cggccccgat ttcaatgaga ttgccttctc cctccgcccc aatcccgacg
      361 aatacttcag cgacgccata gccggcgagg aggccgtgta tgtgctggcg ctcgaagagc
      421 gcattcccgc ccgcgtgccg gaattcgcgg aagttgagga ccaggtgcgt gaaatcgcaa
      481 ccgccgtcgc gaagaaagaa gcccttgacg aactggtcca gagtgtccgc gaaagcgccg
      541 aacaggccgt cagggaggga aacacctttg cttcggtgct tgaatccagc gggctggtga
      601 tgcagaccgc gaccaatgtc tcggccatga ccggcatcga ggatgaacag ttcgccgact
      661 acagcgacct gatcatgcgg gcggccatca ccagcaatcc gggagaaatt cccgaggcca
      721 tcgaaacgga agagggcttc atcctctgcc atatcgcctc gcgtacgccg ggggatgcct
      781 ccctgttcga aacctaccgc gccaatctgg tgaccgcgct gcggcaggac cgcctgggcg
      841 tgaacttcgg cgcctttcag gaatggcttc tgaatgaggc tgacttccag ccccgctcct
      901 tcggcgaatg ggacgatgag gagccggaag aagaggacga ggaatacgcc gacgaggaac
      961 tgtacaagga agaaattccg gttgaggccg acgaaactgc ggctgaatag ttcagcattg
     1021 aactgagtac agcggcacca gcacttcatc cgccgacatg ccgccgtgat aggcgacgtg
     1081 gaaccggggc tcccatcccg gaagtgtcgc ggtgaaggca tagcccggcc tggccatcag
     1141 gaagaaatcc cccgccctgt tcgccaaagc cttgtgaggc ttgcccggcc caaaggcgtt
     1201 catggccagc aggacctcgc ccggcgtgca tacacaggca tggcccagtt tcctgtccac
     1261 gatttccttg aaagccttca ccttgcccgg ccgcacgaag caggaaacag aacgggcatc
     1321 gccggacgtg atcgtggcca ggcagccgta gaatccggga accgcgccca gatctatgcg
     1381 ctgtgcagcg ggcgcgtcaa tgagtccgtg atccgccagc acaaacaggc gcgtgtccgt
     1441 gccctgcagt ttgcgcacca ggcgcgcgag ggattcgtca atctggcgca gatgccggtc
     1501 cgtcttcctg tgcgtcaccc cgtgctcatg gcagttggaa tcatattcgg gccagtaggc
     1561 ataggccatt ttccgccccc gcttcctcac aaaggccatg atctgccgct caagcccctg
     1621 cagcgtgcgg gcggccatgc ggtcggtcca tcgggtgccc gcccttgaat agcggctctg
     1681 cggaatatcc tcgtacgaga tcagtcccct gcggcctttc acgctgtcta tgtacgaggg
     1741 cagatcaaga taggcatgca gatcaaactc ctcgcgggcc agcgggcttc cggtgcgggt
     1801 gatggccgga agaatcatcg tcacgacgcc gaggtcgtgc aggttcaggt accagctcag
     1861 caccgcatgc tcagccgggg atgcgccggt ggcaaaggtt gtcacggccg cggcggtcgt
     1921 cgcggggaaa acggaggaaa tggtcgtcca gggatgcttt gcaaaaaact gcttcccctt
     1981 ccccatgctc aggaaccgct caagctggct gacccccagt ccgtccacga ccagataaat
     2041 gacattgcgc gcctcgcgca gccggcgcgg gtccaggccc ttgaggtttt tgtgcggcga
     2101 ttttccgccg cgcgaacgga tgatggaagc cagcagattg acggtgctgc cgtcagcgta
     2161 gtccggcaga taaaaatctt tcagaaggcc ttttgtgatg tacggcatgc gtggaatcta
     2221 tcccaccgga cccgcttatg caagggaacc gtgcgcacaa gcggattcat tttctctttt
     2281 cagcggccgg ccgccgtgat cgcatcgtat acaatgccgg tttcaatgaa accgccggag
     2341 gattcagcaa acagcccggt ccgggtcacc gcttcctcga tgcgttcctt taacgcgcgg
     2401 ggaatgacga gatcacagca ttcccgggcg cgggacgcca tggcaacttc cccgccccct
     2461 tcgtgcgggg cgggcacacg ccgggaaagg agcgaaagcg tcgcgccgcc cgcgccggca
     2521 tccagcgcgg cgcgcacatg agctgacgat gtgccctctt cgcaaatcag cgtgaagcgc
     2581 accaggcctt caggctccgc tccgcgatga tgccgggccg ccgcggacat tctccgccag
     2641 tcggtggatc cgcgaagctg atcaagcgtg ctgatgacct gctccatggt cgcgacatgc
     2701 ttgcgccggt ccagatgcat ccgcgaattc acggccagtg cgcgcacggg aacgcggcac
     2761 agaaaaccgt ttcccggctc gtgcaggttc gctttccgca cggccacatc catcacaaaa
     2821 tccgagtctc ccggcggaac aacaaacaga aggatttcct tctcctgcgg gatggtgatg
     2881 cgcaggagcc cgagcctgtc gcgcatgccc atgccctcgc cgaaactgac gatcggcacg
     2941 cagagcccca tttccagcat ggtgcgggcc agttctcctc cgcggccgcg ctgcacgatg
     3001 cagtagatca tgtcataggg ggccggctcc atccggttct caacaaccca tgagggctgg
     3061 tcggcgcaga gcttctcctc atcccatggc tgcaccccat ccccgatgag agccacatcc
     3121 tcggcataga cgctcccgcg gcccggcagg tagagatccg cggccgcagc caggcggatc
     3181 atcgccgcgc gttcgtgctg ccgcgggaca tagaagcgaa tgatgtcgga gggcgactcg
     3241 gccattggaa gccgccggcc gaacggctgc cagacggagc gcgccttgag ggcaacctgc
     3301 ttgccgcgtg ttgaccaggt ctcccgcagt ccaatgcggt cagcctcggc ttccagccgt
     3361 tccgtcagcc gcacatcggc aatgcagaca atcctggaga catccatccg tttcacggct
     3421 ttgcctcccg ttctgccggg gccgcatcca tctgcagatt ttcaaggtcg agcgcgtttc
     3481 gccgggcggc gatcagaaaa ccggaaagca gcaccgcgat gatcggaaac acggaggcca
     3541 tggtcaccac gccgaaagcc tcggccgcgc cggcgcgctg gccaatgccc aaacctgcgg
     3601 caataaccag cggaaccgtg atgggaccag tcgttacgcc cgcgcagtcc catgcaatgg
     3661 ccgacatttc ttccgtcgaa atgcaggtca gcgcaagagc cacgaggtaa caccccgcga
     3721 gaagggggat catgggccag gcgaacagta ttcggcagaa tcccatggcc atgccggcgc
     3781 ccacgcccag ggccacggtc actatgagga aagagcgctt gtaggttccc gtcgtcagct
     3841 cttccagtgt cacgcccagc gcgttcagtg aaggttccgc aatggttgcg cccaaaccca
     3901 tcacaaacac aaagagcagc accagcgcat agccccagaa atcctcatcg ccggccacag
     3961 gaccctgttc gggaatccag cggtactcgg cccggtccgc atcgtaccgt tcgcggatga
     4021 acggcacaaa ctgcggcccg cccttgtccg ccagcggaag atactctgcg acggagccgt
     4081 tggggcggac ggcgcgcacc agaaggttct catttatgcc gctgtaggtc acggccttgt
     4141 ccgggcgctc cgtggcctcc cacgcgcgcg gcagagagcg gccggcctga tttccaaggc
     4201 ttgaaagacc gcgatccatg ccgtaactga acagcagcat cccaaccagg ctgaacacca
     4261 gccccagcga aacctcgtcg ttgtgcggca gtcgctcacg cacaatcaac agcagcgcaa
     4321 gaagcagcac ggcggccagc gggacaatgg ctttcagagc gttgacggca tgggccctca
     4381 tcatcttgcg cggcacatgc cggcgcgggc cggcatctga gggcttctcc ttggattccg
     4441 tcccgaaacg ggttttgacc ttctcgggag acagattgtt tgccgccagt tcgcgaaaag
     4501 cctcttcgct tccggccacc cgcaccgact gattgtgacg ctccggcgca aagaactcat
     4561 ccggtccgcc gggcatcggg aaccgcgggg ccagcacagc gccgagcagc aggaccatga
     4621 tcaccggaag cgccgaagcc agcgtaacaa cacccaggcc gctggagggt tcatcgccgc
     4681 ggccggcaat gcgtgaaact ccaattccca gcgcgatcac cagcggcacg gtcaccggcc
     4741 ccgtggtcac ccccccggta tcccacgcca gtccgatgat ggcggccgtg cgcggatcac
     4801 gtgaaaccca gtacgagagc gccagaagaa cgggaatcgt caagtacagg aaaggcttga
     4861 aactccacga gcgcagggac cggaagaccc caagcaccac ggcaaagcca accccgacgg
     4921 ccaccgcgcc cacggtgagc ggcgcgccgg cgttcaggag ataatacagc agcggcgcgc
     4981 gccagggcag agtggaactc ccctgcgcct tcaggaatcc tatggccggc tccgcatagg
     5041 tggcggtaat tccgaggatc accgcaaacg ccacaagcag gcccagaccg gcacgcgcgg
     5101 gcatgcgcag tccgcagcgt tccccgaggg gcatgatggc cagaaacagc ccttccatga
     5161 aaaaagccag acccgccacc acggcaagaa tacccgcggc gattttcaga aaatccgcaa
     5221 tcggggcgcg cagcacgaga agctgaaaga gcgccagata tcccgtgatg aaggccaccg
     5281 catgcacctg gcgcatcagc cgctcccggg catacgtcca gatcaacgag agttttctcc
     5341 gcagcgggat ctgcagcatc acggatgtcg actgtacgga ttcaggcacc acatgctcct
     5401 ggttgccggg tcgcggacac atccgcgcca cttgcggcat tattgtgtaa caaatcgccg
     5461 attcttattc aagtttcttc gcggtggagg catttcaggg catcaggggg gcaagaagcg
     5521 cgagcatgcc ccaggcaatc aggcacgcga ccagcgatga cagcgcaccg atcttgagcc
     5581 atcggaaaaa gctgatctga atcctgcatt ccttttcaag catcccgagc gcaacgatgt
     5641 tggcggtgct gccgatcagc gtgatgtttc cgccgaaaca ggccccgaaa agaagcgccc
     5701 accacaacgg catgattttc acgctttccc cgagcgcttc aatgatcggc gcaaaggcgg
     5761 ccacaaagat gacattgtcc acaaaggctg agcccagggc cgtggtggcc atgatcacgg
     5821 ggatcagcac attcaggtcc gtcccgaaac ggcgggtgaa gccgtccgcc atcaggcgcg
     5881 tcacgccggt ataagccagg gttccggcaa tggcaaacag cagcatgaag aagagcagcg
     5941 tccaccagtc cacctcggct tcgacatagt gccgggcgcg gtcgcgcttg aagatcatga
     6001 taacgccggc gcaggccaga ggagcaacca gcagaatgct gttgggatcc agatgcagag
     6061 cctcttccag cgtatgatgc agcgcaataa acacaagggt cagcagaaga accgccagtc
     6121 cccggccgta aggcaccttc accttcggga ccagactcag gtcgcggtcc atccggtcct
     6181 tgagccgcac atcaaattca tccaggtccc ggcgatacca gaacatcgtg acggccacgc
     6241 aggagcccag ggccagcagc atgacgggaa atgcccagcg gatgaaatca gcgaaactga
     6301 gatgggcctt ggtgccgatg taaataccca cgggattgcc catcattgtg ccggagctgc
     6361 cgatgttcgt gcagagcacg caaatcatga ggtagggcgc gggattcagc ttgaggcggt
     6421 tgcagacctg aaagatcagt gttgaaataa agatgattga agtgacttca tccaccgcgc
     6481 aggccagcag ggccgaggcc acggccgtaa tggccatgaa tttgcgtccg gtgatcttcg
     6541 gaatggaaat gatgctctgg acgatccatg tgaaaaagcc cagatcgcgc agcgcgccga
     6601 ccacaatcat catgccgacc agaaaaagaa taaccggaag cgacgcctct ttgacgaaat
     6661 ggtcaatatc gagcgaccgg gtgaatatca gcactccaat gccgaggaat gcaatggcga
     6721 gacggaaatt ccagaaaaaa agcgtgccga gaataatcgc gagaaacacc gaggtggcca
     6781 ccgcctgcga aaagttcaac cccatccgga cgccgcacag acccgttgtc ccgctgatga
     6841 agagcagcag ggccattcgg accatcatct gcctgagtgt cagttcgccc ccgtggtcat
     6901 ctccgccatg catgatatat tgctccgtga tttttaagaa atgatgaaaa aggacagacg
     6961 catcaggccc agaacagttt ggtcgtgaat cccgccaggc tgaccacgcc ggccagccgc
     7021 cggccttcca gcacctgaat ctgacgggtg tgcttcataa tgaaaatctt ggccagctga
     7081 accgccggcg tatcgggaga cacggacaca taatcctcat gcatgaagtc ggccagcttg
     7141 gtttcattgt ccttcttcat catctcggca aacggctcga agcgcagaat gggagacaaa
     7201 tcctccatcc agagcaggtg ctgcgggagg ctctggctca gcagatcctc catgcagatc
     7261 accccccgga gatctccctg gtcatccacc acggaaatat caagaagtcg acgggagcac
     7321 agcgcggtaa tggccgcccg cagtgtatcg ccttccttca gggtcagcgg ccgggcattc
     7381 atgatgtccg ccgcagtcag atactctcct tgcccccggt ccccggaacg aaagagcgcc
     7441 gataccgtct ctgcagaggc ttccttgata aactccctga tcttcagatc cttgagggcg
     7501 ccggacagca gggccagcac ccgcagatag gagctgggat cgttcttcgg tgtcagcaca
     7561 agcagaatca gattgaccgg atccttgtct gcgtcaaaaa caatgcctcc gcgggttgtc
     7621 gctaccgcga tcagcgtctt gtccatattg tccagccggg catgcggaag cgccactccg
     7681 ggcgcaataa ccgtgggcag cacagcctcg cgctccttga gcgcatgcag ggcctcctgc
     7741 ggatcgaacc cgtccagggt gctctccaga tgattcacca gcagggtaag gacttcatca
     7801 cgcgtggtcg cggcgacctc aaatagaatg ttctccggac tcaggcaatc cctcagttgt
     7861 gtatccatat tctgctccaa atataaaaag tgagtgtacc gctctgccgc acaacccgca
     7921 atgctgttgt gcaatccaat tcgtgacacc ggagcatcat ggccctgcac cagagtttgt
     7981 cctcacttcg ttcgggaaaa cacggtgcag gactcatgaa ccagagcctg gggttttaaa
     8041 cagtggcttt cttgtctcat ggctggccat gaaccatgcc cggagcgcag cggagggctc
     8101 tggttcatgg cacccccggc agggctcgaa cctgcgaccc acagattaga aatctgttgc
     8161 tctgtcctgc tgagctacgg gggtaacaat cagcgcgaat gctaatcaca ctcccgcctt
     8221 ttcggcaatt gttttgtgtt taggattcgc catttcccag tttgatgact agtatgtctt
     8281 caacctgcag gaaaggatat gccatgccaa gccagtcttc aagagagcgg ggaagcagcc
     8341 ccgctgggat tttcatcaag ctcatcctgc tgctgatcgg agcggccgtg ctgctggcgc
     8401 tggcggcgga tgtgtcctcc tggattgaca agcagaaaac agatccggtt cctcccccgg
     8461 aaactgaaat ccagagcatc attctccaga gccatcccag ggtgttccgg gatgtcaatg
     8521 gactgtttac aatgaacgcc ccggacgggt gggagatcac ggcctggccg gacagcaccc
     8581 cgtacaatgt gacttttcaa agccgcaacg gcccctccat ttcgatcacc gccaccaggg
     8641 tggaatacaa cagcctggtg acgctgcgca agcgcattga ggctgttcag agggattacg
     8701 gcctcaacat gaatattgcg cagacaaact ggaatggacg cgagattatc cagcgcacga
     8761 ctgttctgca cgcatcgaaa gttcggacgc tggactttgt cgaaaattat gtcggtcaca
     8821 acatccagat cagcatcccc cactcgctct atgaccgtta tctccccgtg ctgctggatg
     8881 tggcggaatc ctacaagccg ctgaagtaat tcttcagccg
//
LOCUS       DAOQWR010000113         8866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8374_length_8866_cov_5.232550, whole genome shotgun sequence.
ACCESSION   DAOQWR010000113 DAOQWR010000000
VERSION     DAOQWR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_8866_cov_5.232550"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKK36_09010"
     CDS             <1..443
                     /locus_tag="PKK36_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNR94730.1"
                     /translation="NTRFIELAGEVNSGMPQWVIGKVADALNERGKSVKGSKVLVLGL
                     AYKNDVDDDRESPSVELLELLKAKGAKIAYSDPHIPKFRPMRKHNFNLKSVKLTAKSL
                     ASFDCVLLATAHKAFDYALIKKHAKLIVDTRGKYRRPSSKIVRA"
     gene            complement(444..1181)
                     /locus_tag="PKK36_09015"
     CDS             complement(444..1181)
                     /locus_tag="PKK36_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94731.1"
                     /translation="MNILLALLLILIPLGAAAETVYLTDNSSSFDLGVFDVNILADDI
                     HLEHASIITNISTLMAIAGVQECGLWVFDSLNAAPLLYHSFTNITSLTDADMRLYSFS
                     MNLPVPKDFFVGFSAQGDGWNANNSDIIKGATNVLLGVSSNSRTYYYGAVAGGQLTTE
                     YPVANPAFFTIQIKEAPLRISAIHPGNEGIELSVTNFYPDSTNRLERSTDGGNWESVT
                     NFIAGPTGAGWIDTNAAGHTLYRLRRE"
     gene            complement(1178..2047)
                     /locus_tag="PKK36_09020"
     CDS             complement(1178..2047)
                     /locus_tag="PKK36_09020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR94732.1"
                     /translation="MRRYGFILLGMLAAAQAPADLLARFRTAAGDIIVQLYEYEKPVT
                     VGNFVRLVEADAYRNTFSHRCEPDFIVQGGGFITQNPASTNLFELYGFVPHFGPITNE
                     YAVGRQYSNVYGTIAMAKLDGNPDSATSQWFFNLGDNSSGLNSSNNGGYTVFGRVVSG
                     FNVLQDFNSLSISNGIVDLTQFYGPGASAFRNLPVIYSGHVPPHYNELIYVNIHMIRT
                     ENIAITNGLQIISWTGATGLTNHLETTGDMLSGNWTRLLTTNATGAAVTTVITNPPGG
                     SAFYRICVEIPSE"
     gene            2199..4310
                     /locus_tag="PKK36_09025"
     CDS             2199..4310
                     /locus_tag="PKK36_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94733.1"
                     /translation="MGLGLWLLGALHTQGHFIPEPLLRLLYWLVPPALLLAVLWFKSP
                     RRREDDLKRLAPALILCGCTLLFLFSSASRHLWFYFVRWLPFEVKPLSAGAERFVLIT
                     ALLTPLFVLRWRRTWLLLLVIFLYAQFASLDALLSKTGGEALYRTDHPSFMFRLHEFS
                     STFPSFVNYNPFWNAGSLHYYSVTTGSAGPGLILMPWLKLEPTHLMYTCGLAFLFILL
                     VPWISAGAVRLAGGDKSAAFAGGILAMGVSQHYFLWMLHYGTIGAALTASMLVPVAAI
                     FFRIVWMNRTSRLLGAGLALSSFFLLLWPPGALMGAGFGLSFLLCFRKWTRRKWMFLI
                     LCGIVVLVLYIPFISVLMNEGGSVVQYVLHKKGAEDAAKTAFHVDWAMVARGGRHLIA
                     HLQEFHPLLIFLGLIGVFTASRRSIRWWYGPAILYLAFLAGWGHELKPNLQLSRMAIP
                     LAFACVVPAAMLVSRLLRTRDIRLALVRSGLVALLAVTGCNLSLVYGNKSNAPYRIKD
                     ENVDALVHAIRRHTPENARVMFAGKTVHAYGRGQIAYLPILAEREMMACDYYNFPMDT
                     VEYNFPPAPFRKPESNFDTFVNAYNVSTVLTYHEDWKQYFRSRADQYQEVWTWEYVSM
                     FTRKREARPLLAGEGSVTADFNRIRVHLDRPADEVILAYNWLDGLKAEPPVEIYPHTV
                     TNGIQLIGARPGGAADFTIRF"
     gene            4392..5597
                     /locus_tag="PKK36_09030"
     CDS             4392..5597
                     /locus_tag="PKK36_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94734.1"
                     /translation="MKHILIAGLFLLALAGSSALAQIRVTKSAGTKSGIDLTGIQAAG
                     DQASALFMTTLREDLVRSGWFTITGRGQGQFRALGSVSLRGGSLRVECQLYSAAASRA
                     LMSQSYDHAANDARRLAHRVADDIVYAATGRKGIASGRIAMVGNASGHKELYLCDADG
                     GNLVQLTSDRTVSIAPKWSPDGRQLLYTSFLKRFPDVYLVTLQSGNRTAIANYPGLNT
                     GAAMSPDGKDVALILSKDGNPELYVKNLRSGRLTRITETPRAAEASPSWSPDGQRIVY
                     VSDQTGKPHLYAVSRSGGRPERLPGRGSENVAPDWGRNGLIAFCSRTGGNYHVMVLDP
                     RTGETRQISSGYGDFEDPSWAPDGRHIVCSHAQNYRSRVIILDIMGDTPVALTDYKGD
                     WMSPAWSPE"
     gene            5637..6149
                     /locus_tag="PKK36_09035"
     CDS             5637..6149
                     /locus_tag="PKK36_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR94735.1"
                     /translation="MKKFKSLLIVCLCAGVALSTTGCGKKKKGGLRDGDIGGLTGTDI
                     YRNPLGARPGEDMEYARQFEPVYFAYDSSQVDASERGKIEGVAESLRRSANTGVIVEG
                     HCDERGSREYNMALGERRALAVRAYLIGLGIDGSRIQTKSFGEESPSAPGHDEESWRL
                     NRRAEFVLFY"
     gene            6216..6473
                     /locus_tag="PKK36_09040"
     CDS             6216..6473
                     /locus_tag="PKK36_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNR94736.1"
                     /translation="MKILAFFSLTPGGGEILVIMLAMLLLFGAERLPGLARMLGRALA
                     AMRRGLDEVRREIEDSAKVPTIGKEDRKSSNDWKKPPEKFQ"
     gene            complement(6480..7700)
                     /gene="coaBC"
                     /locus_tag="PKK36_09045"
     CDS             complement(6480..7700)
                     /gene="coaBC"
                     /locus_tag="PKK36_09045"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNR94737.1"
                     /translation="MSISVKQAPPVVLCGICGGIAACKAAEVVSSLTRLEMDVRVIMS
                     RAAQEFVTPLTFQALTGKPVTVDMFARPEADRKDQIYPHLYPSADAALFVMLPATADM
                     IARVVRGEAEDVMVASVLGLPPDCIRVFCPAMNTTMWKQDSVQRNVAELEAQGWIRIG
                     PGAGLLACKAEGEGRMAEPADIVRELAAGMKSAQSLRGRQVLILSGPTREHIDPVRFI
                     SNPSTGMMGRALAQEALSRGAQVDFISGPVDPAVLPRGRNIRIHSVTSAQEMLAAAKK
                     HFSSADIILFAAAVADYAPAGAHTRKMPKQQDRIQLELEPTPDIAATLCRTKTGKQTA
                     VGFALQDEDGEEKAREKLQGKNLDAIVLNRLEAVGSSDAVYKFLKAGDSSFEEWDLMT
                     KRGCARRIFEKILQ"
     gene            7806..8099
                     /locus_tag="PKK36_09050"
     CDS             7806..8099
                     /locus_tag="PKK36_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94738.1"
                     /translation="MDINFNCPECGQNLDAPADMVGCIIDCPICSKEIRIPSPSEGGR
                     MHSEPLPQAQEPVEPPAGQTADEDAGKGVTTRIELPPDFAAPKRERKMFIKRR"
     gene            8207..>8866
                     /locus_tag="PKK36_09055"
     CDS             8207..>8866
                     /locus_tag="PKK36_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNR94739.1"
                     /translation="MKYSRSLPVVLLPLVLFALAAQSQVVQDTLSDPAAGVEVTADRL
                     EYLQDQQLLIGIGNVVASQGLDILKADRITVHTETQNAQATGHVVLERAGQVWEGDEL
                     VYNFATRQGEFGEFSGYLEPFYINASEFHRLGDEQYEFNDVRITTCEGDSPGFFLRAS
                     SARMENSIIRAKNVVMYLGGLPIMFIPFYSVDLEKSNIDVLPGYGSRVGAYLLTAFNY
                     RL"
BASE COUNT         1759 a         2526 c         2686 g         1895 t
ORIGIN      
        1 tgaacacccg cttcatcgaa ctggccggcg aagtcaacag cggcatgccg cagtgggtca
       61 ttggcaaagt ggctgatgcg ctcaacgagc gcggtaagtc agtcaagggg agcaaagtgc
      121 tggtgctggg cctcgcctac aaaaacgatg tggatgacga ccgcgaatcg ccgtcggttg
      181 agctgctgga actgctgaaa gccaagggcg cgaagattgc ctattccgat ccgcacatcc
      241 cgaagttcag gccgatgcgc aaacacaact tcaacttgaa gagcgtcaaa ctaaccgcga
      301 aatcgctcgc ctccttcgac tgcgtgctgc tggccaccgc gcacaaggcg tttgactatg
      361 ccctcatcaa aaagcacgcc aaactgatcg tggacacccg cggaaaatac cggcggccgt
      421 catccaaaat tgtccgggcc tgattactcc ctgcggagac gatagagtgt gtgtccggct
      481 gcattggtgt caatccagcc ggctccggtt ggccctgcaa tgaaatttgt gacggattcc
      541 cagttcccgc cgtctgtgct gcgctccaga cggttggtgg agtccggata gaagttggtg
      601 acggatagtt caatgccttc atttcccgga tggattgccg agatgcggag cggagcttcc
      661 ttgatctgga tcgtgaagaa ggccggattg gccacgggat actccgtggt gagttgtcct
      721 cccgccacgg cgccgtaata ataagtgcga ctgttgctcg aaaccccgag cagtacgttt
      781 gttgccccct tgataatatc tgaattattg gcgttccagc cgtcaccctg agccgaaaac
      841 ccgacgaaga aatcctttgg gaccggcagg ttcatgctga atgaatacag ccgcatgtcg
      901 gcatcggtca gggatgtgat gttggtgaat gaatgataaa gcagcggcgc ggcattcagg
      961 ctgtcaaaaa cccacagtcc gcactcctga accccggcaa tggccatcag cgtggagatg
     1021 tttgtgatga tcgaagcgtg ttcaagatga atgtcgtcgg ccagaatatt gacatcaaac
     1081 acccccaaat cgaaagaaga ggagttgtcg gtcagataaa ccgtctcggc cgccgcaccg
     1141 agcggaataa gaatcagcaa taaggccagt aggatattca ttcggatgga atctccacac
     1201 agatgcggta gaacgcggac ccgccgggag ggtttgtaat gaccgttgtg acggcggctc
     1261 cggttgcgtt ggttgtcagc agccgggtcc agtttccgga cagcatgtcg ccggtggtct
     1321 ccagatggtt ggtcagtccg gttgcaccgg tccacgagat tatctgcagg ccatttgtga
     1381 tcgcgatatt ctccgtccgg atcatatgaa tgttcacata aatgagttcg ttgtagtgcg
     1441 gaggaacatg tccggaatag ataaccggaa gattgcgaaa ggcggatgcc ccgggaccat
     1501 agaactgtgt cagatcaaca ataccgttgg agatggataa tgagttgaag tcctgaagta
     1561 cattgaatcc tgacactact cgtccgaata ccgtgtagcc gccattgttg ctgctgttga
     1621 ggccgctgga attgtcgccc agattgaaga accactgcga tgtggctgaa tcaggattgc
     1681 cgtccagctt agccatggcg attgttccat aaacattgct gtactgtctt ccgacagcat
     1741 attcattcgt tatggggccg aaatggggaa caaatccata aagttcgaac agattggtgc
     1801 tggcggggtt ctgggtaata aagcctccgc cctgaacaat gaaatccggc tcgcagcggt
     1861 gggaaaaggt gttccggtac gcgtcggcct ccaccagccg gacaaaattg ccgacagtga
     1921 ccggtttttc atattcgtag agctgaacga tgatgtcccc ggccgctgtg cggaagcggg
     1981 ccagcagatc ggcgggggcc tgcgccgctg caagcatgcc cagcagaatg aatccgtatc
     2041 ttctcatgaa tatgcggagg ctaatggaaa tgcccgacag gtcaagaaaa gagcatggtt
     2101 gccgaaacag ggcggaaaag ctatcgtgtg cggcaatgaa gatgtgctgc agaggagaat
     2161 cacggggagt ctgctggtgc gggaggagcc tggcggccat gggcctgggc ttgtggctgc
     2221 tgggggccct gcatacgcag gggcatttca tcccggaacc gcttctgcgc ctgttgtact
     2281 ggcttgtccc gcctgcgctc ctgctggccg tgctttggtt taaaagcccg cggcggcgcg
     2341 aggatgacct gaagcggctg gccccggcgc tgattctctg cggctgcacg cttcttttcc
     2401 tgttttcaag cgcgtcgcgg catctgtggt tctatttcgt gcgctggctc cctttcgagg
     2461 tgaagccgct ctcagcaggc gcagaacgct ttgtgcttat tacggctctg cttactccgc
     2521 tcttcgtgct gcgctggcgg cggacatggc tgctgctcct ggtgatcttt ctctatgcgc
     2581 agtttgcctc gctggatgcg ctgctgtcca aaacgggcgg cgaggccctg taccgcacgg
     2641 atcatccgtc gttcatgttc cgcctgcatg agttcagcag caccttcccg agttttgtga
     2701 actacaaccc gttctggaat gcgggctcac tgcattatta cagcgtgacc accggaagcg
     2761 ccgggccggg gctcattctg atgccatggc tgaaactgga acccacccat ctgatgtaca
     2821 cctgcgggct ggccttcctt ttcattctgc tcgtgccatg gatatccgcc ggggcggtgc
     2881 gtctggcggg cggcgacaag tctgctgcct ttgcgggcgg cattctcgcg atgggggtca
     2941 gccagcacta tttcctgtgg atgcttcatt acgggacgat cggcgccgcg ctcaccgcgt
     3001 ccatgctggt tcccgtggcc gcgattttct tccgcatcgt ttggatgaac cgcaccagcc
     3061 ggctgctcgg agcaggcctt gcgctcagct cttttttcct tctgctctgg cctccgggcg
     3121 cattgatggg cgcgggattc ggcctgtctt tccttctctg cttccggaaa tggacccgcc
     3181 gcaaatggat gttcctgatc ctttgcggaa tcgttgtgct cgttctgtat atccctttca
     3241 tctcggtgct catgaacgag ggcggcagcg tggttcagta tgtgctccac aagaagggcg
     3301 cggaagacgc ggcgaaaacc gcatttcatg tggactgggc catggtggcg cggggagggc
     3361 gtcatctgat tgcgcatctg caggaattcc atccgctgct gattttcctg ggactgatcg
     3421 gcgtcttcac cgcaagccgc agaagcatac gctggtggta cggcccggcg attctttatc
     3481 tggcattcct tgcggggtgg gggcatgaac tgaagcccaa tctgcagctc tcgcgcatgg
     3541 cgatccctct tgcctttgca tgcgtggttc cggccgcgat gctggtgtcg cggctgctgc
     3601 gcacgcggga cattaggctg gcgctggtgc gcagcgggct ggtcgcgctg ctggcggtca
     3661 ccggctgcaa cctgtcgctg gtctacggga acaagagcaa cgcgccctat cgcattaaag
     3721 acgaaaatgt ggatgcgctg gtccatgcga tccgccggca tacgcccgaa aatgcgcgcg
     3781 tcatgtttgc gggaaaaacg gtgcatgcct acggccgcgg acagatcgcc tatctgccca
     3841 tcctcgccga acgtgaaatg atggcctgcg attattacaa tttcccgatg gacaccgtgg
     3901 aatataattt cccccctgcg cccttccgga aaccggaaag caatttcgac acgtttgtca
     3961 acgcatacaa cgtcagcacc gtgctgacct atcacgagga ctggaagcag tatttccgtt
     4021 cccgcgcgga ccagtatcag gaagtgtgga catgggaata cgtatccatg ttcacgcgga
     4081 agcgcgaagc ccggcccctg ctggcgggag aggggagcgt aacagctgat ttcaaccgca
     4141 tccgcgtgca tctggaccgg ccggcggatg aggtgatcct tgcctacaac tggctggatg
     4201 gtctcaaggc ggaacccccg gtggaaattt atccgcatac ggttacaaac gggatccaac
     4261 tgattggggc gcgtccgggc ggcgcagcgg atttcacgat acgcttctga ccggcccgcc
     4321 ggtttttccg gccgcgcaag cttgatgcgg gggggataat ttcatacagt ccgcttctta
     4381 tgaaaaccca tatgaaacat atcctgattg cgggactttt tctgctggcc cttgcgggct
     4441 cttccgcgct tgcgcagatc cgcgtgacta agagcgccgg aaccaagtcg gggattgact
     4501 tgaccggtat tcaggcggcc ggcgaccagg cctcggcgct gttcatgacc acgctccgcg
     4561 aggatctcgt tcgttccggc tggttcacca taaccggcag ggggcagggc cagttccggg
     4621 cgctcggttc ggtctcgctc cggggcggga gtcttcgcgt ggaatgccag ctctacagcg
     4681 cggccgcttc gcgcgcgctg atgagccagt cctacgacca cgcggcaaat gatgcccgcc
     4741 gtctggcgca ccgcgtggcc gatgacattg tctatgcggc aaccggccgc aaaggcattg
     4801 cctcgggccg catcgcgatg gtgggcaatg cgtccggaca caaggagctt tatctgtgcg
     4861 atgcggatgg aggaaacctg gtgcagctca ccagtgaccg caccgtcagc atcgcgccga
     4921 agtggagtcc tgacggccgt caactgttgt acacatcatt cctgaagcgc ttccccgatg
     4981 tgtatctggt taccctgcag tctggaaacc gcacggcgat tgccaactat cccggcctga
     5041 acaccggcgc ggccatgtcg cccgacggaa aagatgtggc gctgatcctt tccaaggacg
     5101 gcaatcccga gctctatgtc aagaatctgc ggagcggccg tctgacgcgc atcacggaaa
     5161 cgccgcgcgc ggccgaggcc agcccgtcct ggtcaccgga cggccagcgc attgtctatg
     5221 tttctgatca aaccgggaaa ccgcatctct atgcggtctc gcgcagcggc ggccgccccg
     5281 agcggcttcc cggccggggc agcgaaaacg tggcgccgga ctggggccgg aacggactga
     5341 tcgccttctg cagccgcacc ggtggaaact atcatgtcat ggtgctcgat ccgcgcacgg
     5401 gggaaacgcg ccagatatca tccggctacg gcgatttcga agatccctcg tgggcgcccg
     5461 acggccgcca tattgtctgc agtcacgcgc agaattaccg ttcgcgcgtc atcattcttg
     5521 acattatggg ggacacccct gtagctttga cggactataa aggggactgg atgtccccgg
     5581 catggtcgcc tgaatgattt gagtttttgc ggtttaactg aatgaggaaa gaaaaaatga
     5641 agaagtttaa atccttgctg attgtttgcc tctgcgcggg tgtggcgctg tccaccaccg
     5701 gttgcggaaa gaagaaaaag ggcggactcc gtgacggtga tatcggaggc ctgacgggca
     5761 ccgacatcta cagaaatcct ctgggcgcac gcccgggcga ggacatggag tacgcgcgcc
     5821 agttcgaacc ggtgtatttc gcgtatgaca gctcacaggt ggatgcttcc gagcgcggca
     5881 aaatcgaagg cgtggcggaa agccttcggc gcagtgcgaa caccggcgtg attgttgaag
     5941 gtcattgcga tgaacgcgga agccgcgaat acaacatggc cctgggcgaa cgtcgtgccc
     6001 tggctgtgcg cgcgtatctg atcggccttg gcattgacgg aagccgaatc cagaccaaga
     6061 gcttcggcga agagagcccc tcggctcccg gccatgacga agagtcgtgg cgcctgaacc
     6121 gccgtgcgga attcgtgctg ttttattaaa cattgaatca gaacctgatt tgaaaacccc
     6181 gccggcttcc ggcggggttt tctgctcaaa gccttatgaa aatcctggcc tttttcagtt
     6241 tgacgcccgg cggcggggag atcctcgtga tcatgctggc catgctgctg ctgttcggag
     6301 ccgaacggct cccgggtctt gcgcgcatgc tgggccgggc tctggcggcc atgcgccggg
     6361 ggctggatga agtgcgtcgt gaaatcgagg attccgcaaa agttccaacc attggaaaag
     6421 aggaccggaa aagttccaat gactggaaaa aacctccgga aaagttccag tgactggaac
     6481 tactgcagga tcttttcgaa tattcttcgg gcgcagccgc gcttggtcat cagatcccac
     6541 tcctcgaaag atgaatctcc cgccttcagg aacttgtaca ccgcgtcact ggagcccacg
     6601 gcttccagcc ggttgagcac gatggcatcg aggtttttcc cctgaagttt ttcgcgcgcc
     6661 ttttcctcgc cgtcttcatc ctgcagcgcg aacccgacgg cagtctgttt tccggttttc
     6721 gtccggcaga gcgtggcggc gatgtcgggc gtgggctcca gctcaagctg aatgcggtcc
     6781 tgctgcttgg gcatcttgcg ggtatgcgcg ccggccgggg catagtcggc cacggccgcg
     6841 gcaaacaaga tgatgtcagc cgacgaaaag tgcttttttg cagccgcaag catttcctgt
     6901 gcggaagtga ctgaatggat gcggatgttc ctgccgcgcg gaaggacggc aggatccacc
     6961 ggaccggaaa taaaatccac ctgtgcgccg cggctcagag cctcctgtgc aagggcccgg
     7021 cccatcatgc ccgtgctggg attgctgata aagcgcaccg ggtcaatatg ctcgcgcgtg
     7081 ggcccggaca gaatgagcac ctgacggccc cgcaggctct gcgcggattt catgcccgcg
     7141 gcgagttcgc ggacgatatc cgccggttcg gccatgcgcc cttcgccttc ggccttgcag
     7201 gccagcagcc ccgcgccggg gcctatccgg atccagccct gcgcctcaag ctccgccaca
     7261 ttccgctgaa ccgaatcctg cttccacatc gtggtgttca tggccgggca gaagacgcga
     7321 atgcagtccg gcggaagtcc cagcacgctg gcgaccatca cgtcttccgc ctcgccgcgc
     7381 accacgcggg cgatcatgtc tgccgtggcc ggaagcatca caaagagcgc ggcgtctgca
     7441 gagggataaa ggtgcggata gatctggtct tttctgtcgg cctccggccg ggcaaacatg
     7501 tccacggtga cgggttttcc ggtgagcgcc tgaaaggtga gcggcgtcac gaactcctgc
     7561 gcggcgcggc tcatgatgac gcgcacatcc atctccagcc gggtgaggct gctgaccact
     7621 tccgccgcct tgcaggcggc gatgccgccg cagattccgc agagaacgac ggggggcgct
     7681 tgctttacgg atattgacat gaccatacct ctagcataag ttgaccgcat cttcaagtgt
     7741 gcttgcaaag cggaagataa aagcttaatt tctttcagca gaatgaagag agggaacgaa
     7801 acgtcatgga tataaatttt aattgtcccg agtgcggtca aaatctcgat gcgccagccg
     7861 acatggtggg atgcatcatt gactgcccga tctgctccaa ggaaatccgt attccgtctc
     7921 cttccgaggg cgggcgcatg cattcggaac cccttcctca ggcacaggag ccggttgaac
     7981 ctcccgccgg acagacggcg gatgaagacg cgggtaaagg cgttaccacc cgcatcgagc
     8041 ttcctccgga cttcgccgcg cccaagcgcg agcggaagat gttcatcaag cgccgctgag
     8101 aacgccccgg tgcgcgcggc tgttcctgaa aatcctgttg gcttcacctg cgggttgtgc
     8161 taggttgccc cgcactgttt caagttaagg acatgcgagg ttaaagatga aatacagccg
     8221 ttctctgccc gttgtgctgc tccctctggt gctgttcgcc cttgcggcgc agtcgcaggt
     8281 tgtgcaggat acgctctctg atcccgccgc gggagtggag gtcaccgccg accgcctgga
     8341 gtatctgcag gatcagcagc tgctgatcgg catcggcaat gtggttgcca gccagggtct
     8401 tgatatcctg aaagcggacc ggatcacggt gcacacggaa acccagaatg cgcaggcgac
     8461 cggccatgtt gttctggagc gggccggaca ggtgtgggag ggcgatgaac tggtctacaa
     8521 ctttgccact cggcaggggg agtttggcga attcagcggc tacctggaac ccttctacat
     8581 caatgcctcg gagtttcatc gcctggggga tgagcagtat gagttcaacg acgtgcgaat
     8641 caccacctgc gagggtgatt ctcccggctt cttcctccgc gcctcttccg cccgcatgga
     8701 gaacagcata atacgggcca agaacgtggt gatgtatctc ggaggactcc cgatcatgtt
     8761 tattcccttt tattcggttg atcttgagaa atccaacatt gatgtgctgc ccggctacgg
     8821 gagccgtgtc ggcgcctacc tgctgacggc cttcaactac agattg
//
LOCUS       DAOQWR010000114         8852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8390_length_8852_cov_5.941571, whole genome shotgun sequence.
ACCESSION   DAOQWR010000114 DAOQWR010000000
VERSION     DAOQWR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8390_length_8852_cov_5.941571"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PKK36_09060"
     CDS             <1..1590
                     /locus_tag="PKK36_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNR94740.1"
                     /translation="EVIDTVTGTPALIPLAGPANRQGRIAAENICGRSTKYTTTQGTS
                     VVKVFHMTGGGTGATEKVLKRRGIKYHKAYIHPPGHAEYYPGTAAMHLKLLFADDGKI
                     LGAQVVGYDGIDKRIDVLATALRAGLTVYDLQELELAYAPPYGSAKDPVNMIGFVAAN
                     MLQGDTEVWYAEDFPKKTQDGTIVDVRPKTAYDLGHIPGAVNVPLGQLRERMDEIPKD
                     KPVYLYCKVGFTSYLAYRILKQKGWPRAANLSGGMLTFRSWHGDEQAPGVPEAPLVSY
                     PAGTSGEIASTGRTLELDACGLQCPGPIMKLRDTAAGMNAGDELVVTASDPGFFSDAP
                     AWCRRNGHELLDITRDGMKITARIRVGGARATAVAPPAGSSKKTFVVFSGDLDRVLAA
                     FVIANGAVAMGSEVTLFFTFWGLNALRRGNPPPVEKGLLDRMFGMMMPRGAGALKLSN
                     MHMMGAGTKMMRHVMKTKGVKSLPELMADAKASGVRLVACSMSMDVMGITKEELVDGV
                     DVGGVAAFLEEADQSGTTLFI"
     gene            1651..2730
                     /gene="aroC"
                     /locus_tag="PKK36_09065"
     CDS             1651..2730
                     /gene="aroC"
                     /locus_tag="PKK36_09065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNR94741.1"
                     /translation="MGNTFGRLFRVTTFGESHGIGVGAVVDGCPPRMELSEKDIQPQL
                     NRRRPGQSDLTTPRDEKDSVTILAGTEKGKTLGTPIALLVRNADQRPEDYGEVTDIPR
                     PSHADFTYMQKYGIHASSGGGRSSARETIGRVAAGAIAEKFLKEKFGIEIVAWVRAAG
                     PVDAGDLSTGSFTRSDVDRTPVRCPDLKIAEKMTALIKETRDRKDSVGGIVTCICRNV
                     PPGWGEPVFDKLEAMLAHAMLSIPATKGFEIGSGFSGTTMNGSAHNDPFVLKGKKLGT
                     ATNRSGGVQGGISNGEPIVFNVAFKPPATIGLPQKTCDKAGRETVLEAKGRHDPCVVP
                     RAVPIVESMAALVLADLALLQASRG"
     gene            complement(2781..4007)
                     /locus_tag="PKK36_09070"
     CDS             complement(2781..4007)
                     /locus_tag="PKK36_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94742.1"
                     /translation="MKRCLFTAVLICLFVSSGALAAEKKSPKKAEKKAEPKVEKKAEK
                     NSPSKWSLSAGVRSRDLDVEFSAAAPSSYADWASLVRRPRNTGSTDIDNGTTPRTDYE
                     DGEVVFDPSWTWVDGTSLFGMDNDAQLGIRDVFNTVTFGSTRTRQWSELSQTALEGED
                     DTTKGGAYVAARRAIGDKGLGCLVQYGINVFEIDSGWQETARQTIMERQTRYATTYDI
                     YGWGSAVTPAPFEGGDAFEWVVVDAALHNAFYGWMGLGDAMEPRQTQDARQRTLAVLT
                     ASSKTDLDVDVHDLLLGLEWALPIGDWLSLSLAAGPTLNVISWDLEHTTEWVNQTTGA
                     ALQTMQEKDGDDEFEVGAAAQVTVRLNLDKKGRVFCEGSAAYNWVDKIDASTESASAE
                     IDVSSFSGSAGIGIRL"
     gene            complement(4103..4723)
                     /locus_tag="PKK36_09075"
     CDS             complement(4103..4723)
                     /locus_tag="PKK36_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94743.1"
                     /translation="MNLAELIGYAGSVIILVSLLMKSLLKLRWINLAGSILFSLYGLM
                     IKAWPVFGINAVIVCIDAWFIFQMIRRWDYFDLVPLSEIGTAYFRKFFLYYENDIRSF
                     FPDVVFDSLTGSETYILFRNMAPVGFFALRFEGEEARVVGDYVAPEYRDFKTGKFVYD
                     VKRMYFKEKGIKRFVADFGKDIHSKYLIRNGFRTDDGYPDLLVKHL"
     gene            complement(4730..7366)
                     /gene="alaS"
                     /locus_tag="PKK36_09080"
     CDS             complement(4730..7366)
                     /gene="alaS"
                     /locus_tag="PKK36_09080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR94744.1"
                     /translation="MMTAAEVRQSFLDFFKDKEHVVVPSSPVVLPSDPTLLFTNAGMN
                     QFKEIFLGARESQFRRVADTQKCIRVSGKHNDLEEVGIDTYHHTFFEMLGNWSFGDYY
                     KREAISWAWELLTRVWKLPRERLWATVYRADDEAAELWKTCTDIDPARILRFDEKDNF
                     WEMGDTGPCGPCSEIHYDRSEKGCGPEMVNAGSPECIEIWNLVFIQYNRKSDGSLTEL
                     PAKHVDTGMGFERVVSVIQGKNSNYDTDVFQPVISAVANLSGRKYEGRDAVAMRVIAD
                     HLRALSFAIADGVLPSNDGRGYVLRRLLRRAVRYGRNIGLKDPFMRKLFPVVESMMAG
                     VFPELKTNRENIMRALNAEEESFAATLDRGIAIFDETVAELKAKDELTFPGDAAFKLY
                     DTYGFPLDLTVLMAHEQGLVVDQERFNELMEAQRNRARGARRDAEMAKEMDQISALVA
                     AGIRSQFTGYTNLEDESEIICILRDGKACDRLTAGDEAEIMLAKTPFYAESGGQVADL
                     GVIRGASGEFHVMDTQKPADGIILHIGRVVQGSFTKGENVFAEIDKERRGRIRRNHTA
                     THLLNYALRSLVNPSIKQAGSLVTPERLRFDFSHYEAIPEDKIAEIEHLVNDCIMQST
                     PVETYEMALKDVHGSGIVAVFDEKYGDIVRVLDVGGYSRELCGGTHVRNTGELGLFRI
                     VAESSIASGVRRIEAVSGWPAYEWTRREHELVRSLAHRFSSTSEEIPQRVDTLVSQNK
                     KLEKELKAAATQSALSRTDELLTQQQTVGGLALLAADLGELPAENLREVMDSLRQKMP
                     SGAIVLGSRADGKACFTAGVSADLISRGLHAGQLIKAVAAIAGGGGGGQPQKAQAGGK
                     HPEKVAEAIAKAPGIVQGMIKG"
     gene            complement(7369..8445)
                     /gene="recA"
                     /locus_tag="PKK36_09085"
     CDS             complement(7369..8445)
                     /gene="recA"
                     /locus_tag="PKK36_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNR94745.1"
                     /translation="MAARAASKEKDTDKKTPSALSAVLAQIQKEYGEGAIMRLGADEG
                     HKQIPSISTGALTLDLALGIGGVPRGRVIEIFGPESSGKTTLVSHIIANAQKKGGLAA
                     FIDAEHAFDPMYARKIGVNLDDLLISQPDSGEEALNIAETLVKSNSLDVVAVDSVAAL
                     APQAELDGAMGSAHVGLQARLMSQALRKLTAAINKSRTCCIFTNQIREKIGVMFGNPE
                     TTPGGRALKFYASVRLDIRRVSVLKDTAGNVYGSHTRVKVVKNKVAPPFTQAEFDILY
                     AEGISWTGSILDAAIQYDLIDKRGSWLAYEGQQLGQGREAAKAFLAENREMADAMVAK
                     ITEKAHSNPSIRGSMAVTGADVSE"
     gene            complement(8449..>8852)
                     /gene="thpR"
                     /locus_tag="PKK36_09090"
     CDS             complement(8449..>8852)
                     /gene="thpR"
                     /locus_tag="PKK36_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNR94746.1"
                     /translation="ADALKPKLDEIAAKYSVHRCRLWGLGTFGPERAPRIVWVGLDCP
                     DPLFRMQKETADACRELGIGLEARDFHPHITIGRIRSARHADSLTSAARSDRNTPCDP
                     CRFDAMLLMASRPGPQGSRYTILNRSELEGE"
BASE COUNT         1717 a         2642 c         2656 g         1837 t
ORIGIN      
        1 gaggtgattg acaccgtgac gggaaccccg gcgctgattc ctcttgcggg gcctgcgaac
       61 cggcagggcc ggatcgccgc ggagaacatc tgcggacgca gcacgaaata caccacgacg
      121 cagggcacct ccgttgtgaa ggtttttcac atgaccggcg gcggaacggg cgccacggag
      181 aaggtgttga agagacgggg aatcaaatat cacaaggcct acattcatcc tccgggccat
      241 gcggaatatt atccgggcac ggccgccatg cacctgaagc tcctgtttgc cgatgacgga
      301 aagatcctcg gcgcgcaggt ggtcggctat gacggcatag acaagcgcat tgacgtgctg
      361 gccaccgccc tccgcgcggg cctgactgtt tatgatctgc aggagctgga actggcctac
      421 gccccgccgt acggctccgc caaggatccg gtgaacatga tcggctttgt ggccgccaac
      481 atgcttcagg gcgacaccga agtctggtat gccgaggatt tcccgaagaa aacgcaggac
      541 ggaaccattg tggacgtgcg gcccaaaacc gcctacgacc tcggacatat tcccggcgcg
      601 gtgaatgttc ccctgggaca gctccgcgag cgcatggatg agattccgaa ggacaaaccg
      661 gtttatctgt actgcaaggt gggcttcacc agctatctcg cgtaccggat tctgaagcag
      721 aagggctggc cgcgggcagc caatctgagc gggggcatgc tcactttccg cagctggcac
      781 ggcgatgaac aggcccccgg cgtgccggag gccccgctgg tttcgtatcc tgccggaaca
      841 tccggcgaaa tcgcctccac gggcaggacg cttgaactgg atgcctgcgg cctgcagtgt
      901 ccgggcccga tcatgaagct cagggacacc gcggccggaa tgaatgccgg cgacgaactg
      961 gttgtgaccg ccagcgatcc gggtttcttc tccgatgcgc ccgcgtggtg ccgcagaaac
     1021 ggtcatgaac tgctggatat tacccgtgac ggcatgaaaa tcaccgcgcg catccgtgtg
     1081 ggcggcgcgc gcgccacggc cgtcgcgccc cctgcgggat cttcaaagaa gacctttgtg
     1141 gtcttctccg gcgatctgga ccgggtgctc gccgcgtttg tcatcgccaa cggcgcggtg
     1201 gccatgggca gcgaggtgac gctgttcttt actttctggg gcctgaatgc tctgcgcagg
     1261 ggcaatcctc cgccggtgga gaaggggctg ctcgacagaa tgttcggcat gatgatgccg
     1321 cgcggagcag gggcgctgaa gctatccaat atgcacatga tgggcgcggg cacaaaaatg
     1381 atgcggcacg tcatgaaaac aaagggcgtg aaatcccttc cggagctcat ggccgacgca
     1441 aaggcgtccg gcgtgcgcct ggtcgcctgc agcatgagca tggacgtgat gggcatcacg
     1501 aaggaagagc ttgttgacgg ggtagatgtc ggcggcgttg ccgcgttcct tgaggaagcc
     1561 gaccagtccg gcacgacatt gttcatttag ccatcgttct tgcaacctgg ccggacttgt
     1621 gtacgctgat gggcgagttg gagaatggtc atgggcaata catttggacg gttgtttaga
     1681 gtaacgacgt ttggcgagtc gcacggcatt ggggtcggtg ccgttgtgga cggctgcccg
     1741 ccgcgcatgg agctttcgga aaaggacatc cagccgcagc tgaaccggcg tcgcccgggc
     1801 cagagcgacc tgaccacgcc ccgtgatgag aaggacagcg tcaccattct ggccggaacc
     1861 gagaaaggca aaacactggg gaccccgatt gccctgctgg tccgcaacgc ggatcagcgt
     1921 ccggaggatt atggcgaagt gacggatatt ccccggccgt ctcatgcgga tttcacttac
     1981 atgcagaaat acgggattca cgcctccagc gggggcgggc gctccagcgc ccgcgagacc
     2041 attggccgcg tcgcggccgg ggccattgcg gagaagttcc tgaaggagaa attcgggatt
     2101 gaaattgtgg catgggtccg ggccgcggga ccggtggatg ccggagacct ttcaacgggt
     2161 tctttcacgc gttcagatgt cgacaggacg cctgtgcgct gccccgacct gaagatcgct
     2221 gaaaaaatga cagcgcttat taaggaaacc cgcgaccgga aggattcagt cggcggcata
     2281 gtcacctgta tctgccgcaa tgtccccccc ggctggggcg agccggtgtt cgacaaactg
     2341 gaggccatgc tggctcacgc catgctttcg atccccgcga cgaagggttt tgaaatagga
     2401 tccggatttt ccggcacgac catgaacggt tccgctcaca acgatccgtt tgtgctcaag
     2461 ggaaaaaaac tcggcaccgc gacaaaccgc agtggcggag tccagggcgg gatatcaaac
     2521 ggggagccca tagttttcaa cgtggctttc aagccgccgg cgaccattgg actgccgcag
     2581 aagacctgcg acaaagcggg ccgcgaaacc gtgctggagg ccaaaggccg ccacgacccc
     2641 tgcgtggttc cccgcgccgt gccgattgtt gaatccatgg cggcgcttgt gctggcggac
     2701 ctggccctgc ttcaggcttc ccggggataa aagagcggcc cggcttgcgc cgggccgttc
     2761 tcattcaatc ctgcagcagt ttagagccgg atcccgatac cggccgaacc ggagaagctg
     2821 ctgacgtcaa tttccgccga tgcggattcc gtgctggcat caattttgtc cacccagttg
     2881 taggcggcgg aaccttcgca gaacacgcgg cctttcttgt ccaggttcag gcgcaccgtc
     2941 acctgtgcgg cggcgccgac ttcgaactca tcgtcgccgt ctttctcctg catggtctgc
     3001 agggcggcgc ccgtggtctg attgacccat tccgtggtgt gttccagatc ccacgagatc
     3061 acgttcagcg tggggccggc ggcaagcgag agggacagcc agtcgccgat cgggagcgcc
     3121 cattcaagtc ccagcagcag gtcatgcaca tccacatcca ggtcggtttt tgagctcgcg
     3181 gtcagcacgg ccagcgtgcg ctgccgggca tcctgcgtct ggcgcggttc catcgcgtct
     3241 ccaagcccca tccatccgta aaatgcatta tgcagcgcgg catccacaac cacccattca
     3301 aaggcatcgc cgccttcgaa gggggcgggg gttacggcag agccccatcc gtaaatgtca
     3361 tatgtggttg cgtaccgggt ttgccgctcc atgatggtct ggcgtgccgt ttcctgccag
     3421 ccgctgtcaa tttcgaacac attgatgccg tactgaacca ggcacccaag ccctttgtcc
     3481 ccgatggcgc gccgtgcggc cacgtaggct ccgcctttgg tggtgtcgtc ctccccttcc
     3541 agtgcggtct ggctcagctc cgaccactgg cgggtccggg tggaaccgaa cgtaactgta
     3601 ttgaacacgt cgcgtattcc gagctgggca tcattatcca tgccgaagag cgatgtgccg
     3661 tccacccatg tccatgacgg atcaaaaaca acctcgccgt cttcatagtc cgtgcgcggg
     3721 gtggtgccgt tatcaatgtc agttgaaccg gtgttgcggg gacgtcgaac cagcgatgcc
     3781 cagtctgcat aggaggaggg cgccgcggca gaaaattcga catccagatc gcgggagcga
     3841 acccctgcac tgagggacca tttggaaggg ctgttttttt cggccttttt ttcgactttg
     3901 ggttcagcct tcttctcggc tttcttgggc gacttttttt ccgcggccag cgcaccggaa
     3961 gaaacaaaca ggcatatcaa aactgctgtg aacagacatc ttttcatgac agtctcctca
     4021 attatgttgt aacggacctc actgcgaaga tgctagaaga agccctgtcg tgagaaaagt
     4081 aaaaaatcag aaaaaatcag gatcagagat gcttgaccag caggtcggga tatccatcat
     4141 cggttctgaa gccgttgcgg atcagatact ttgagtggat gtctttccca aaatcggcca
     4201 cgaagcgctt tatccctttt tccttgaagt acatgcgctt gacatcatag acaaacttgc
     4261 cggttttgaa gtcgcggtac tccggggcca cgtaatctcc gaccacgcgg gcttcctccc
     4321 cttcaaaacg gagcgcgaaa aaacccaccg gcgccatgtt gcggaaaaga atataggttt
     4381 cggatccggt cagtgaatca aaaacgacgt ccggaaagaa tgagcggatg tcattctcat
     4441 agtagaggaa gaactttctg aaatatgcgg ttccgatttc cgaaagcggc acgaggtcaa
     4501 aatagtccca ccgccggatc atctgaaaaa tgaaccatgc atctatgcag acaatgaccg
     4561 cgttgatccc gaacaccggc cacgctttga tcatcagccc gtacagtgaa aacagaatgg
     4621 aaccggccag attgatccac cgcagcttga gaagcgattt catcagcagg gagaccagaa
     4681 tgatgactga tcccgcatat ccgataagtt ctgccagatt catgaattct taacctttaa
     4741 tcataccctg cacgatgccc ggcgcttttg caatggcttc tgcgaccttt tccgggtgtt
     4801 tgcctccggc ctgcgctttt tgcggctgtc cgccgccccc accgccggcg atggcggcca
     4861 cggccttgat cagctgtccc gcgtgcagcc cccgtgaaat cagatcagcg gaaacgcccg
     4921 ccgtgaagca ggccttcccg tcggcgcggc tgccgagcac aatggcgccg gacggcattt
     4981 tctggcgaag ggaatccatg acctcgcgca ggttttcggc cggaagttcg ccgagatcgg
     5041 ccgccagcag cgccaggccc ccaacggtct gctgctgcgt cagcagttca tccgtgcggg
     5101 aaagagcaga ctgggtcgcc gcggccttca gttccttctc aagtttcttg ttctggctga
     5161 ccagtgtgtc cacccgctgg ggaatttcct cgctggtcga gctgaagcga tgggcgaggc
     5221 tgcgcacgag ctcgtgctcg cggcgggtcc attcataggc cggccagccg ctgactgctt
     5281 cgatgcgtcg cacgccggaa gcgatggagc tttccgccac gatccggaag agccccagtt
     5341 cccccgtgtt gcggacgtgg gtgccgccgc acagctcgcg gctgtatccg ccgacatcca
     5401 gcacgcggac gatgtctccg tatttttcgt cgaacacggc cacaatcccg gatccatgaa
     5461 cgtccttcag ggccatttca taggtttcaa ccggagtgct ttgcatgatg cagtcgttga
     5521 ccagatgttc gatctccgca attttatctt cgggaatggc ttcgtagtgg ctgaaatcga
     5581 agcgcaggcg ctccggcgtg accagtgacc cggcctgctt gatggagggg ttcaccagtg
     5641 accggagggc atagttcaga agatgcgtcg cggtgtgatt gcggcggatg cgcccgcggc
     5701 gctccttgtc aatttccgca aacacattct ctcctttggt gaaagagccc tgaaccaccc
     5761 ggccgatgtg caggatgatt ccatcggcgg gtttctgggt gtccatgacg tgaaactcgc
     5821 cgcttgcgcc gcggatcacg ccgaggtccg ccacctgccc gcccgactcg gcatagaaag
     5881 gtgttttggc cagcatgatc tccgcttcgt cgccggccgt cagccggtca caggctttgc
     5941 cgtccctgag gatgcagata atctcgcttt catcctcgag attcgtgtag ccggtgaact
     6001 gtgaacgaat gccggccgcc accagcgccg agatctgatc catttccttt gccatttcgg
     6061 catcccggcg cgcgccccgc gcgcggtttc tctgtgcttc catcagttca ttgaagcgct
     6121 cctgatcaac cacaaggccc tgctcatgag ccatgaggac cgtgagatcc agcgggaagc
     6181 cgtaggtgtc atacagcttg aaggccgcgt cgcccgggaa ggtgagttcg tccttcgctt
     6241 tcagttcggc gacggtttca tcgaagatcg caatgccgcg gtccagtgtg gcggcgaaac
     6301 tctcttcttc cgcgttgagc gcgcgcatga tattttcgcg gtttgttttc agctcgggaa
     6361 agactcccgc catcatgctt tccacgacgg ggaacagctt gcgcatgaag ggatccttca
     6421 ggccgatgtt gcgcccgtag cgtaccgcac gcctcagcag gcggcgaagc acatagcccc
     6481 ggccgtcgtt tgacggcagc accccgtccg cgatggcaaa ggagagcgcc cgaagatgat
     6541 ccgcaatcac gcgcatggcc accgcgtcgc gcccctcgta cttgcgtccg ctcaaattgg
     6601 ccaccgcact gatgacgggc tggaaaacat ccgtgtcgta attggaattt ttcccctgga
     6661 tgacggacac gactcgctcg aagcccatgc cggtgtccac gtgtttggcc ggaagttcgg
     6721 tcaggcttcc gtcagacttg cggttgtact ggatgaaaac gaggttccat atctcaatgc
     6781 attccggaga tcccgcgttc accatttccg gtccgcagcc tttttcggag cggtcataat
     6841 ggatttccga acaggggccg cagggcccgg tgtctcccat ctcccagaag ttgtcctttt
     6901 catcgaaccg caggatgcgg gcggggtcta tgtccgtgca ggttttccag agctcggccg
     6961 cttcgtcgtc ggcgcggtac acggtggccc acagccgttc cctgggcagt ttccacaccc
     7021 gggtgagcag ttcccatgcc caggagatgg cttcgcgctt gtagtagtcg ccgaacgacc
     7081 agtttcccag catctcaaag aaggtgtggt gataggtgtc tatgcccact tcctcaagat
     7141 cgttgtgctt gccgctgacg cggatgcatt tttgcgtgtc cgccacgcgc cggaactggc
     7201 tttcgcgcgc gcccaggaaa atttccttga actggttcat tcccgcattg gtgaagagca
     7261 gggtggggtc cgaggggagc accacgggcg aactcgggac gacgacatgc tccttgtcct
     7321 tgaagaaatc gaggaaggac tgtctgactt cagctgcggt catcatgatt attcactcac
     7381 atcggcgccg gtcaccgcca tggatccgcg gatggaggga ttggagtgcg ctttttccgt
     7441 gatcttcgcc accatggcat ccgccatctc cctgttttcg gcgaggaagg ccttggcggc
     7501 ctcgcggccc tgcccgagct gctgcccttc gtaggcgagc cacgaacccc gcttgtcgat
     7561 caggtcgtac tgaatggcgg catcaagaat gcttcccgtc cacgaaatgc cttcggcata
     7621 gagaatgtcg aattccgcct gcgtgaacgg cggggccacc ttgttcttca cgactttcac
     7681 gcgggtgtga cttccataca cgttcccggc ggtgtccttg agcacggaca cgcggcggat
     7741 gtcgagtcgc accgaagcat aaaacttgag cgcccgtccg cccggcgtgg tctcgggatt
     7801 gccgaacatg acgccgatct tttcgcgaat ctggttggtg aagatgcagc aggtcctgga
     7861 tttgttgatt gcagcggtca gcttgcgcag cgcctgcgac atcaggcgcg cctgcagccc
     7921 gacatgagcg gagcccattg ccccgtccag ctccgcctgc ggggccagcg cggccaccga
     7981 gtccactgcc accacgtcca gagagttgct cttcaccagc gtttcggcga tgttcagggc
     8041 ctcttcgccg gaatcgggct gggaaatcag cagatcgtcc agattgacgc cgattttccg
     8101 ggcatacatg gggtcaaagg catgttcggc gtcaatgaac gccgccagtc cgcccttttt
     8161 ctgcgcattg gcgatgatgt gcgaaacgag ggtggttttt ccggaggatt ccgggccaaa
     8221 gatctcgatc acacggccgc ggggaactcc gcctatgccc agcgcgaggt ccagggtcag
     8281 cgcgccggtg gaaatggagg gaatctgctt gtggccttca tcggcgccca gccgcatgat
     8341 ggcgccctcg ccatattcct tctgtatctg cgccagcacg gcgctgagcg cggacggggt
     8401 cttcttgtcg gtgtcttttt ccttcgatgc tgctctggcg gccatggtct actctccttc
     8461 taattccgat ctgtttaaaa ttgtgtagcg cgagccctgc ggccctggcc gactggccat
     8521 cagcagcatg gcatcaaacc ggcaagggtc gcagggtgta tttctatccg agcgggcggc
     8581 cgatgtcaat gaatccgcat ggcgcgcaga acggatgcgg ccaatggtga tgtgggggtg
     8641 aaaatcgcgc gcctcaagcc cgattccaag ctcccggcag gcgtctgctg tttccttctg
     8701 catcctgaaa agcggatcgg gacagtccag tccaacccag acaatccgcg gcgcgcgttc
     8761 ggggccaaaa gttccaagac cccagagccg gcagcggtgc acagaatatt tcgcggcaat
     8821 ttcatcaagc ttcggcttca gtgcatctgc cg
//
LOCUS       DAOQWR010000115         8830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8416_length_8830_cov_5.033960, whole genome shotgun sequence.
ACCESSION   DAOQWR010000115 DAOQWR010000000
VERSION     DAOQWR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8416_length_8830_cov_5.033960"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PKK36_09095"
     CDS             complement(<1..156)
                     /locus_tag="PKK36_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNR94747.1"
                     /translation="MSLNTEQKLRYSRQLILPGFGEKEQERLLRGRVLVIGAGGLGSP
                     ALLYLAAA"
     gene            403..1221
                     /locus_tag="PKK36_09100"
     CDS             403..1221
                     /locus_tag="PKK36_09100"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNR94748.1"
                     /translation="MNSNQFLQFMDKVKIYDLTQRLSVHTPPWPSYMPLGIQYFKRIA
                     GAHMGQGANGQIITTSNHVGTHIDGEIHFHSSGRCIGDVPLKEWVGAGAVADISDMVG
                     DYDLYSPEMIMSKVEVRKGDILIINTGYHKYGWDQKHSDEVRYFVKHPGPDKDFHKWA
                     LKLKLKWIGVDCGSADHPMNTIIRDWHPKAFVEAEKVLKAKTGKTWDEYFPPKEFYQV
                     MHIKLFPAGLVHAENIGGDIGKLGSQRLWIGCYPLKGIEMESAMCRIVAMAQPK"
     gene            1235..2122
                     /locus_tag="PKK36_09105"
     CDS             1235..2122
                     /locus_tag="PKK36_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNR94749.1"
                     /translation="MTIALDYDYAKPATLAQARKLMAKHKAPRVLAGGTDLVPWLIDG
                     MVEPDILVDIKGIASLGRIRVKGDVVTLGALVTFNDLLESKVIAKRAPMLQDMASKVA
                     STGVRNRATVLGNLCSAVPCCDAGPSLTVFDAQIQLSGPDGDRVVPLDDWFTGPRQTV
                     RKPAEIATAISFTLPPKGSGAAWIKLGRYKGEDLAQASVAVLVKPGNVCKVAFGSVAP
                     RPVRGVKIEQLLDGRPLTDDLIAEAVKLLPECISPITDVRSTREYRMQTMEVMLERGL
                     KAAAARAAGRGPKYGTALI"
     gene            2133..2594
                     /locus_tag="PKK36_09110"
     CDS             2133..2594
                     /locus_tag="PKK36_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014557810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNR94750.1"
                     /translation="MKKTITMNLNGDLRSVEVEPGDILLDVLRTRLGIKSPKIGCEKG
                     DCGACTILLNGKVVRSCLILAIEVDGQEVTTVEGIGRNGATRLQKAFVEQNSFQCGFC
                     APGVVLAATALLDKNPKPSESEVKEALAGNLCRCTGYGPIIDAVMSVAKEN"
     gene            2596..5031
                     /locus_tag="PKK36_09115"
     CDS             2596..5031
                     /locus_tag="PKK36_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNR94751.1"
                     /translation="MANYKYVGKDAVRIDGMDKVSGAAVYVDDMDFGPNLLHAEIVES
                     PHAHALIKKVDISEAMKVPGVVKVVTGEDYPFTFGLYMQDRFIFARDRVRFVGEQVAA
                     VIARDSKTAKRAAKLVRVDYEELPDVLDLEGALKKDSVLLHPDLDKYSRCPWFFPRKG
                     TNIAHWRKTRKGDIEEGFAEADEILEDTYTVPRYAHCAIEPHVIVGLYDMAGRLTLWS
                     SSQSPFTQRHVFAEALAPLGLTHKDIRVITPYVGGGFGGKAGVSMEIIGCALAMAVRG
                     QPVKVRLTRAQEFYNTYQRQGVIAKIRLGCKKDGTITALEHTLHWDAGAYVEYGANVV
                     NAVGLSATGPYRVPHVKIDSICTYTNLPPGGPYRGFGYSEFHFGLESHMTRMAKKLGM
                     DPVAFRKKNAIAEGDTNGYGSKMNPGDIQKAIDEVAKAVEWGKKEESADPDKKIGKGL
                     ALFWKAPAMPPNASSAAFLKFNEDGSINIAVSGMEIGQGYLTVMAQVAAEVLAVPPER
                     IRVETPDTDRNAYEWQTVGSHVTWSCGNAVRKAAEDAKQKIFDTIHRGCGLPVDSLYL
                     EDEHVKSRADASFAMPFKNFVINGIMTQDGTWRGGPIVGSGMFMPEFTSATGDPETSQ
                     GGHPCVHYTVGAAAVKLEIDQRTGKMRVLKTALAVDAGQAINPSLVRGQLTGGLLQGL
                     ATVLYEDMRYSRKGKLLNPNFTDYKIPTAEDVPDEIIPIIIETPQPDGPFGARGVGEH
                     TMIPAAPMVADAVEDALGLRIKTMPLTAEKVALAARGIDFGSLKTDSIGFCFMGSDPA
                     DYDFKSRDSAATE"
     gene            complement(5131..7392)
                     /locus_tag="PKK36_09120"
     CDS             complement(5131..7392)
                     /locus_tag="PKK36_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94752.1"
                     /translation="MKKILTALIIPALGLAVQAADFGVYLDSNDGSSALVIYDNQTNQ
                     LMSVQSDGPVETVSNLTVSGIVSAVSFEGDGSDMTGVVTSETDPVWEAEKAGYATGTP
                     VYVEADPVWEAEKAEYATGTPVYVETDPIWEAEKAAYATGTPLYIEADPVFGASVAAS
                     ITGLETGQWTEAYGWGDHELGGYADSADLVSASNALQTAIDTETSARIGADAALSNEV
                     SGIASDLGDEAAARIGADAALSNDVSGIASDLGDETAARIGADAALSNEVSGIASDLG
                     DEAAARIGADAALSNEVSGIASDLGDEATARIGADAALSNEVSGIASDLGDEAAARIG
                     ADAALSNDVSGISSDLSDETSARIGADAALSNAVSGEAAARIGADAALSNDVSGISSD
                     LSDETSARIGADAALSNAVGTLVTDLNTASNALQTDVSTRLDSNFWAAADSTTNYMRL
                     SDGVFPQNMVWVATNGTASGPGSLNAPYDTPQNGYNSAAALFPSQPATLVITAGSYPG
                     LVAGSPNIHVSGISRPQLASLTVTGVTSGIVGSRRIDGIVVTGSVFIGATGVKLDNMR
                     VQNGLTITGQQIEVQNSFITVSSTTPAVDVRPYAQNVAIYNSSIESAQSTNSLKVGDV
                     VTHFEVIGCEIINTAGFLTIDDLASSPPTPIHLYTHNYIKSTGAATSAMQINVVFPPF
                     PPFNASVAFHNNTVWGDLVAKGGAGLHAQFSNNSIYGTNSWLQAGTVTSDGYNNTHHS
                     RSTVQTLPDSWDD"
     gene            complement(7410..>8830)
                     /locus_tag="PKK36_09125"
     CDS             complement(7410..>8830)
                     /locus_tag="PKK36_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94753.1"
                     /translation="LGAGAILQGSGTVSGAGDISGTISPGDGSPDDTATLSFTGDLVF
                     QDGSAFECYAADHTTLDKLVVGGTVSGICEVVLTRDVAAVPLYQVIVDGGTSSDYDLF
                     ISSPTNWVIEESSAVVTSLAAIAVDSISTNANETGADLSISHTTGTGSNRFMLVGISC
                     EDDNASGMSVTSVVYGGSSLSQVVKQVSVQEAISEIWSLAAPPAGAGTVVIGIDEGSA
                     SGVSIAAAVVTFTGVNPVTPLGTATGSIGNSTAASVTVSSASSELVFDNLATDDGRTC
                     AADTGQTERFNFRTETGADGITCAGSTKAGAASVVMSWTLNSLDTWALCSVALKPALI
                     YTPVGDLLASETTGDSDSDTLPDKWEYDNFDDRTAADPDADDDDDLMDNWQERIAGTD
                     PRDDTSLLTLDAVETRSKTNLVISWSSVDGKLYSVYRRTNLVTGTALLLESDLPATPP
                     VNTFTNAVTVWERYYYRVEVQP"
BASE COUNT         1888 a         2463 c         2688 g         1791 t
ORIGIN      
        1 ggccgccgcg agatagagca gggcgggaga tccgagccct ccggccccga tcaccagaac
       61 gcgtccgcga agcaggcgct cctgttcctt ttcgccgaag ccgggaagaa taagctggcg
      121 cgaatatcga agtttctgct cggtattaag agacatcgcg cactccttca aatggcataa
      181 agaacaggct gcacagcata gccggttttg ctggacactt tcatatcata taaaatctat
      241 gtaaataata tatattatgc ggagtgtccg tgcaacagcc tgaagttggc tcaaactgca
      301 atatttctga gcattaaggc tggaatgaag ggacattttg tgtacactcg ttcccattat
      361 gactcatcat gagcccgtaa cagcatacaa caggagaatg agatgaacag caatcagttc
      421 cttcaattca tggacaaagt caaaatctat gacctcactc agcgcctcag cgtgcatacg
      481 cctccgtggc ccagctatat gccgctgggc attcagtatt tcaagcgcat tgccggcgcg
      541 cacatggggc agggcgccaa cggccagatt attaccacca gcaatcatgt gggaacacac
      601 attgacggtg aaatccactt tcattccagc ggccgctgca ttggcgatgt tcccctgaag
      661 gaatgggtcg gcgcgggcgc tgtggcggac atttcagaca tggtgggcga ctatgacctc
      721 tattccccgg aaatgatcat gagcaaggtg gaggtccgca agggcgatat tctgattatc
      781 aacaccggat atcacaaata cggctgggac cagaagcatt ccgatgaggt ccggtatttc
      841 gtgaagcatc cgggcccgga caaggatttc cacaagtggg cattgaagct caagctgaaa
      901 tggatcggcg tggactgcgg ttcagccgat catcccatga acacgatcat tcgcgactgg
      961 catcccaagg cttttgttga agccgagaag gtgctgaagg ccaaaacggg caagacctgg
     1021 gatgagtact tcccgcccaa ggaattttat caggtcatgc acattaaact gttcccggcc
     1081 ggactggttc atgctgaaaa catcggcggc gacatcggga aactgggatc ccagcgcctg
     1141 tggatcggat gctatccgct gaaggggatc gagatggaat cggccatgtg ccgcattgtg
     1201 gccatggcgc agccgaaata aggaggcccg tccgatgacg atcgcacttg actacgacta
     1261 cgcgaagccg gcgacactgg cgcaggccag aaagctgatg gcgaagcaca aggctccccg
     1321 tgtgcttgcg gggggaaccg acctggtgcc atggctgatt gacggcatgg tggagcctga
     1381 catcctcgtg gacatcaagg ggattgccag tctcggcaga atcagggtca agggcgatgt
     1441 ggtcacgctg ggcgctctgg tgaccttcaa cgacctgctg gaatcaaagg tcatcgcaaa
     1501 gcgcgcgccg atgcttcagg acatggcctc gaaagtggcg tccacgggag ttcgcaaccg
     1561 ggcgaccgtg ctgggcaacc tttgctcggc cgtgccctgc tgcgatgccg ggcccagcct
     1621 gacggtcttt gacgcgcaga ttcaactgtc cggtccggat ggggaccgcg tggtgccgct
     1681 ggacgactgg ttcacggggc ctcgccagac cgttcgcaag ccggcggaaa tcgccacggc
     1741 aatatccttc acgctgccgc cgaagggcag cggtgcggcg tggatcaagc tcggccgcta
     1801 caagggcgag gaccttgccc aggccagcgt ggccgtgctg gttaagcccg gcaatgtttg
     1861 caaggtggct ttcggttctg tcgctccgcg tcccgtgcgc ggcgtgaaaa ttgaacagct
     1921 gctggatggc aggccattga cggacgacct gatcgccgaa gccgtgaagc ttcttcctga
     1981 atgcatcagt ccgatcaccg atgtgcgctc tacgcgcgag taccgcatgc agacgatgga
     2041 agtgatgctg gaaaggggcc tgaaagcggc cgcggcgcgc gccgccggca ggggcccgaa
     2101 atacggaacc gcactgatct gaggagaagc cgatgaagaa aacaatcacg atgaacctga
     2161 acggcgacct ccggagcgtg gaggtggagc ccggcgatat tctgctggat gtcctgcgga
     2221 ccagactggg catcaagagc ccgaagatcg gatgcgaaaa aggcgattgc ggggcgtgca
     2281 ccatactgct gaacggcaag gtggtgcgga gctgcctgat tcttgccatt gaggttgacg
     2341 ggcaggaggt caccacggtc gagggcatcg gcaggaacgg agccaccagg cttcagaagg
     2401 cgtttgtcga gcagaactcc ttccagtgcg gcttctgcgc gccgggcgtc gtgctggcgg
     2461 cgacggcgct gctggacaaa aatccgaagc cgtctgaaag cgaagtgaaa gaagcccttg
     2521 ccggaaacct ctgccgctgc accggctacg ggcccattat cgacgcggtc atgtctgtgg
     2581 caaaggagaa ctgatatggc gaactataaa tatgtcggga aagacgccgt caggattgac
     2641 gggatggaca aggtgtccgg cgccgcagtt tatgtggatg acatggattt cggtccgaat
     2701 ctgctgcatg cggaaattgt tgaaagtccc catgcccatg ctctgatcaa aaaagtggat
     2761 atttcagagg ccatgaaggt tccgggggtg gtcaaggttg tgaccggaga ggactatccg
     2821 ttcacgtttg gcctctatat gcaggaccgg ttcatttttg cgcgcgaccg tgtacgcttt
     2881 gtgggcgaac aggtggccgc ggtgatcgcg cgcgattcca aaaccgccaa gcgcgccgca
     2941 aagctggtgc gggtcgacta tgaggagctg cccgatgtgc tggacctgga aggggcgctc
     3001 aagaaggaca gcgtgctgct ccatccggat ctggacaaat attcgcgctg cccgtggttc
     3061 ttcccccgca agggcaccaa catcgcccac tggcgcaaga cccgcaaggg cgacattgaa
     3121 gagggctttg ccgaggcgga tgagattctc gaggacacct ataccgtgcc gcgctatgcg
     3181 cactgcgcca ttgagccgca tgtgatcgtg ggcctctatg acatggccgg gcgcttgaca
     3241 ctttggtctt cgtcacagtc gcctttcacg cagcggcacg tgttcgccga ggcgctggcg
     3301 ccgctgggct tgacgcacaa ggatatccgg gtgatcacgc cctatgttgg cggcggcttc
     3361 ggcggcaagg ccggcgtaag catggagatc atcggatgcg cgctggcaat ggctgtgcgc
     3421 ggccagccgg tgaaggttcg cctgacccgg gcgcaggagt tctacaatac ctatcagcgg
     3481 cagggcgtga tcgcgaagat caggctggga tgcaaaaagg acgggaccat tacagcgctg
     3541 gagcatacgc ttcactggga cgccggcgcg tatgttgaat acggcgccaa tgtggtcaac
     3601 gcggtcgggc tctcggccac ggggccatac cgggttcccc atgtgaagat tgactcgatc
     3661 tgcacctata ccaacctgcc gccgggcggg ccgtaccggg gcttcggcta ttccgagttc
     3721 cacttcggcc tggagtcgca catgacccgc atggcgaaga agctgggcat ggatcccgta
     3781 gctttccgca aaaagaatgc catcgccgag ggtgatacca acggctatgg atccaaaatg
     3841 aatccgggcg atatccagaa agccattgat gaagtggcaa aagccgtgga atggggcaag
     3901 aaagaagaat ccgctgatcc tgacaagaag atcggcaagg gccttgcgct gttctggaaa
     3961 gcgccggcga tgccgccgaa cgcgagttcg gcagcgtttc tcaagttcaa cgaggatggg
     4021 agcataaata tcgccgtctc aggaatggaa atcgggcagg gctatctgac cgtcatggcc
     4081 caggttgccg ctgaagtgct cgccgttcct ccggagcgga tccgcgtgga aacaccggat
     4141 acggaccgca atgcctacga atggcagacg gtcggatcgc atgttacctg gagctgcggc
     4201 aacgccgtgc gcaaggcggc ggaagacgca aagcagaaaa tattcgacac cattcaccgc
     4261 gggtgcgggc tgccggttga ttcgctgtat ctggaagacg agcatgtgaa aagccgtgcg
     4321 gatgcatcct ttgcgatgcc gttcaagaac ttcgtgatca acggtatcat gacgcaggac
     4381 ggcacatggc gcggcggtcc gattgtggga agcggcatgt tcatgccgga gtttacttcc
     4441 gccaccggcg atcccgaaac cagccagggc ggccatccgt gtgtccacta cacggtgggt
     4501 gcggcagccg tgaagctgga gattgatcag agaaccggaa aaatgcgagt gctgaaaacg
     4561 gcactggccg tggatgccgg gcaggccatt aatccatcgc ttgtgagggg ccagctgacc
     4621 ggaggcctcc tgcagggcct tgcgaccgtg ctttatgagg acatgcgcta cagcagaaaa
     4681 ggcaaactgc tgaatccgaa tttcaccgac tacaagattc caacagccga agacgtgccc
     4741 gatgagatca ttccgatcat tatcgagaca ccccagcccg acggtccgtt tggcgcgcgc
     4801 ggggtggggg agcacaccat gataccggcc gccccaatgg tggccgacgc cgtggaagat
     4861 gcccttgggc ttcggatcaa gaccatgccg ctcactgcgg aaaaggtggc tctggccgca
     4921 cgcgggattg atttcggcag cctgaagacg gactcaatcg gcttctgctt catgggcagc
     4981 gacccggcgg attacgattt taagtccaga gactccgccg cgactgaata aagcgggcac
     5041 tttaaaggcg gttgcgcctg taacagcaca acaaacctgt gccgccagtc agcacggaac
     5101 ccgtggtcta tccggattca acactgcacg tcagtcgtcc cacgaatcgg gaagcgtctg
     5161 tacggttgaa cggctgtggt gtgtgttgtt ataaccgtct gatgtaaccg ttccagcctg
     5221 cagccatgag tttgttccat agatggagtt gtttgaaaac tgggcatgca aacctgcgcc
     5281 ccctttagcc accaggtctc cccagaccgt attgttatgg aatgctacac tggcgttaaa
     5341 gggaggaaac ggcgggaata caacgttgat ctgcatggcg cttgtggcgg cgcccgtaga
     5401 cttgatgtaa ttgtgagtgt agagatgaat cggagttggc ggggaagagg ccaaatcatc
     5461 tattgtcagg aacccggctg tgttgatgat ctcgcatcca atcacttcaa agtgggtaac
     5521 gacatcgccc accttcagtg agttggtcga ctgtgcgctt tcgattgatg agttatagat
     5581 ggccacgttt tgcgcgtagg ggcgcacatc aacggcggga gttgtgcttg acacggtgat
     5641 aaaggagttc tgaacctcta tctgctgccc ggtgatggtc aggccgtttt gcacacgcat
     5701 attatccagt ttgaccccgg tggcgccgat gaaaacagaa cctgtcacta caatcccgtc
     5761 aatgcgccga ctgccaacga tgcccgaggt gacaccggta acggtcagag aagccagttg
     5821 cgggcgagaa atgccggaca catgtatatt ggggctgccc gccacaagac ccggataact
     5881 tcctgcggta ataacaagcg tggcaggctg cgagggaaac agtgcggctg cgctgttata
     5941 gccgttctgg ggtgtatcat agggggcatt cagcgaaccc gggcccgagg cggtcccatt
     6001 tgtggccacc cataccatgt tctgcggaaa gactccatcg ctgagtcgca tatagtttgt
     6061 ggtactgtct gccgctgccc agaaattgct gtcgagccgg gtgctgacat ccgtctgcag
     6121 ggcgttgctg gccgtgttca ggtcggtcac aagagttcca accgcattgc tcagggcggc
     6181 gtctgcaccg atgcgggcag acgtttcatc actcaagtct gaggaaatcc cgctgacatc
     6241 attgctcagt gcagcgtccg cgccgatccg ggccgctgct tcaccactga cggcattgct
     6301 cagggcggcg tctgcaccga tgcgggcaga cgtttcatca ctcaagtctg aggaaatccc
     6361 gctgacatca ttgctcagtg cagcgtccgc gccgatgcgt gcagccgctt cgtccccgag
     6421 gtccgaggca atcccgctga cttcgttgct gagcgcggcg tctgcgccga tacgggcagt
     6481 ggcttcatct ccaaggtccg aggcaatccc gctgacttcg ttgctcaggg cggcatccgc
     6541 cccgatccgg gcggcggctt catcgccaag gtccgaagca attccgctga cctcattgct
     6601 gagggcggca tccgcaccga tgcgggcggc agtttcatcg ccaaggtctg aagcaattcc
     6661 gctgacatca ttgctcaggg cggcgtctgc gccgatgcgc gcagccgctt cgtccccgag
     6721 atccgaggcg attccgctga cttcgttgct gagggctgca tccgcaccaa tgcgggctga
     6781 ggtttcggtg tcgatggcgg tttgaagggc attgcttgct gacacgagat cagcgctgtc
     6841 ggcatagcct cccagttcat ggtcgcccca tccataggct tcagtccatt gccctgtttc
     6901 aagtcccgtt atgcttgcgg caaccgatgc gccgaaaaca ggatcagctt caatataaag
     6961 aggtgtaccc gtggcataag cggccttttc ggcctcccaa atgggatcag tttccacata
     7021 taccggagtg cctgtggcat attcggcttt ctctgcttcc cagacgggat cagcttccac
     7081 atataccgga gtgcctgtgg catatccggc tttctccgcc tcccagaccg gatccgtctc
     7141 gctggtgaca acgcccgtca tatctgaacc gtcgccctcg aatgagacgg cggacacgat
     7201 tccgcttacg gtgagattgg agaccgtttc aaccggcccg tcggactgga cagacatcag
     7261 ctggttggtc tggttgtcgt agatgaccag cgcgctgctg ccatcattcg aatccagata
     7321 cacgccgaaa tcggcggcct gaactgcgag ccccaatgcc ggaataatca atgctgtcag
     7381 tatttttttc atgatgttgt ctccagaacc tacggctgaa cctcaaccct gtaataatat
     7441 cgttcccaaa ctgtgactgc gttggtaaag gtgttcaccg ggggtgtggc cggcaagtcg
     7501 ctttccagca gcaaggccgt tccagtcaca agatttgtcc ggcggtagac tgaataaagt
     7561 tttccgtcca cgctgctcca tgaaatgacg agattggttt ttgagcgggt ttccaccgca
     7621 tccagggtca gcagcgaggt gtcatcccgg ggatccgttc ccgcaatccg ttcctgccag
     7681 ttgtccataa gatcgtcatc atcgtccgcg tctggatccg cggcggtgcg atcatcaaaa
     7741 ttgtcgtatt cccacttgtc gggaagcgtg tcggagtcgc tgtcgccggt ggtttctgaa
     7801 gccagcagat cgccaaccgg cgtgtagatc agcgccggct tcagcgccac ggaacagaga
     7861 gcccaggtgt cgaggctgtt aagcgtccag gacatgacaa ccgatgccgc tcccgccttt
     7921 gtgctcccag cgcaagtgat cccgtcggcc cccgtttcag tccggaagtt gaaccgctct
     7981 gtttgtcccg tgtccgctgc acaggtgcgg ccgtcgtcgg tcgcaaggtt gtcgaaaacg
     8041 agttcggaag aagccgatga aaccgtgacc gaggcggccg tgctgttgcc gatggagccg
     8101 gttgccgttc ccagcggcgt gaccggattt acccctgtga aggtgacaac tgcggcggca
     8161 atggatactc cgctggcaga cccttcatca atgccgatca ccacggttcc tgctccggcg
     8221 ggaggagcgg ccagactcca aatttcactg atggcttcct gcacggaaac ctgcttgaca
     8281 acctgtgata gggatgagcc gccatagaca acggaagtga ccgacatgcc ggaagcgttg
     8341 tcgtcctcgc aggaaatgcc caccagcata aaccggttgc ttccggtgcc tgtggtgtgg
     8401 gatatgctta gatcggcccc ggtttcattg gcgtttgtag aaatgctgtc cacggcaatc
     8461 gccgccagac tggtaaccac agctgaggat tcttcgatga cccagttggt gggggatgag
     8521 atgaagaggt cataatcgct ggaagttccg ccatccacaa ttacctgata gagcggtacg
     8581 gccgccacgt ctcttgtcag gaccacctcg caaatacccg aaaccgttcc gcccaccacc
     8641 agcttgtcga gcgtggtgtg gtccgcggca tagcactcga aggcgctgcc gtcctggaaa
     8701 acaagatcgc cggtgaaact gagcgtggcc gtgtcgtcgg gggatccgtc gcccggggat
     8761 atcgttccag aaatatcgcc cgcgccgctg acggtgccgg agccttgcag tatagccccc
     8821 gctccaagcg
//
LOCUS       DAOQWR010000116         8824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8425_length_8824_cov_5.519443, whole genome shotgun sequence.
ACCESSION   DAOQWR010000116 DAOQWR010000000
VERSION     DAOQWR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8425_length_8824_cov_5.519443"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PKK36_09130"
     CDS             complement(<1..1471)
                     /locus_tag="PKK36_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR94754.1"
                     /translation="MPLKKSAVPVKKTLKPVKKPAVPVKKTPAPPAVKPSAPSPVEEK
                     AKMSDIDFLREQLREIQEVMGAAEPAAEAPASDKRGFLDIPLNKVLKILPPEFVRPDI
                     AASGQMTVTVIVDDLFDQLKSGRVTTPLRHIISEIPDGYLTPESRQHMEDMMSLPLPL
                     VVSSMEPGELKRRTASRDRELGVRGMPNVFSRDQLTPKAADAMRAAAEPAPVEAPPPA
                     VEEPAPEPEIPAVPEAAVVEETLPEEPVAEEPAPEEPAAEPPAVEEPVFAQEPAGEPP
                     VAEEPVAEEAPAEEEAVEEPVAEEPVAAEPEEEPVAVKEPAEAEEEMSPEAMLQKILE
                     EETPAPEEEAPVEMEAPAAEETAAEEAAVSEEELPEEELLREVPEEPAEAVPVEGEPE
                     AVYYEEPAPVEEAAEPVPEEPVREEAPAGEESALPAAAASAEEVKEGLYLYLRGVDLN
                     QATVEELMSGIGGISHTLAENIVATRQASGQYFYLTDLLR"
     gene            1733..2365
                     /locus_tag="PKK36_09135"
     CDS             1733..2365
                     /locus_tag="PKK36_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HNR94755.1"
                     /translation="MSFINFARKEIAFKIVYYGPALCGKTTNLEQIHAAIVPESRGEM
                     TVLSTQADRTLFFDFLPLKSEIIKGFTSKFQLYTVPGQTIYRETRKLVLRNVDGVVFV
                     ADSQWEKMEENAESFQDLIDNLAEQSVSIDKIPYMLQFNKRDLSNVAPAHYMDFMLNQ
                     RETRVPTMESVACNCLGVFETLNIMAKMILAAFMKENNMAMADVPDDVCL"
     gene            2384..2842
                     /locus_tag="PKK36_09140"
     CDS             2384..2842
                     /locus_tag="PKK36_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNR94756.1"
                     /translation="MQKLTPQLGKKIDEILLNLVVRAEAEAVLLCDRGGNIVAQNTTE
                     RYHQEDNIAALAAGSFYATRELARLIGEPVFRYIFHRGEKTSLYMQTTSIDMLVVVVF
                     GKDSNPGLVKLYGDEACAALEELCAQNAASPEEHSETLELDSTKTMFRRK"
     gene            complement(2854..3972)
                     /locus_tag="PKK36_09145"
     CDS             complement(2854..3972)
                     /locus_tag="PKK36_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94757.1"
                     /translation="MTRKVSQSIYETAASHAPNEKTWREPPAATEAAAWQKEQRRMKL
                     QNWLFLLTVVAFGVLISVVAVQYYMLSRVPPEQARSIFDIEASDSAGYRILTSGGRRK
                     SAPEELLVVDDVADFNPMLPPAGAEVPLSATWVKQAGYYITRAEKAYQDNNPEEALAM
                     YDHVISIFPSIQGVHHYRGLCYLQLGDAAAAAEAFAMASDQDSPGYGLENNLGVSYLT
                     LKDYQKAEEHFLRAVELKPDYSLALLNLGTLYVRTDRPERAVEYYDRYLKLKPGDLQA
                     SQAYAHVLLQQQLWERALPVLTGIVKQAPELAPAYLQLGEALYRTGSTGDAIKSLQKG
                     ASLMDPRNALAQLADPRFDGIRNTDEFRALVRTLGSDE"
     gene            complement(3969..5123)
                     /locus_tag="PKK36_09150"
     CDS             complement(3969..5123)
                     /locus_tag="PKK36_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94758.1"
                     /translation="MDEIVPRAKEIPPATPPGDLYSVQHQDTRRAEPCRLKRPPPEYE
                     GRWKLFRRAAFFAGLMLFVLLVLMAVLKLTTRTRRTSSSRRVGTPPALHEAEERKNSL
                     PGEGPWVSAAAPDPQMMIQAVQALRDADALAVMGGWSEAAAAYRRVLDLWPGYAAADA
                     GLGHALLMQGEWALAGQHLQRAAAADPGRAPVLNDLGRIELIRKNPDGAAIFFGTAAE
                     LDPEHVESHLNLARADYVMGYVDRARKHIHDALEMHPGHPEALRYLAYLEAAAGNYET
                     AMSLLAGAIQHAPDMPSLYTDAAATCALMGNTSKAVDYLRSASERIPPEQVRLIFGEP
                     VFMAARKTSEGQQLEADLVTAVQLKKEAGNQALEDAHSAQQKTEESAEQE"
     gene            5293..6579
                     /locus_tag="PKK36_09155"
     CDS             5293..6579
                     /locus_tag="PKK36_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR94759.1"
                     /translation="MVKGFSRRHALGAGTALVLLANLVIGARIYSQEAAAGKDDQAYE
                     NLALFTRVLEEIRIQYVDEDKTEYQDLIYGALEGMLASLDEHSQFLDPEMYTEMKEET
                     SGQFGGLGIVISLQNGILTVVSPMDDTPGFRAGLTAGDQIIEIDGENMEGSSLQEAVK
                     KLRGTPGTKVSLKIHRPESREYLEMEIERAIINVETVKDARMLDGGIGYVRITQFSEP
                     TAGALQKALDELFEQGMQALVLDLRYNPGGLLTSAVEVSQKFLKKNKVIVSTRGRDEK
                     EAQVFRARGREHYADLPLAVLVNSGSASASEIVSGALQDHNRAIIIGEKTFGKGSVQS
                     VLPQQDGSAIRLTTSKYYTPDGRVIHEKGIEPDIRVSMPHEVWQKLMLRKQSTEQTGG
                     EDVKDVQLERAVDVLRGIRIFQTQNRKGIFTARSGG"
     gene            6584..7627
                     /gene="tsaD"
                     /locus_tag="PKK36_09160"
     CDS             6584..7627
                     /gene="tsaD"
                     /locus_tag="PKK36_09160"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNR94760.1"
                     /translation="MIVLGIETSCDETAVALVENGLKIHAHSVYTQIAAHRPYGGVVP
                     EIACRSHVEILPGLIRDTVADSGLAWDRIDAVAVTYGPGLASSLLVGVAAGKALALRL
                     EKPLIAVNHLRAHIYSLFMGREDLSPKQVYPLLILLVSGGHTCLVRADSLNECHLLGQ
                     TLDDAAGEALDKGASVLGLGYPGGPAIEKAAWDGDPGFVRFPRGLRHGAQSSLAGSLE
                     RDLCFSFSGLKTSLRYYVHDHPDVVAGENLKNVAASYQAAVIDALVERLERALARHDV
                     KAVACVGGVARNQVLRDKLTELAEARKLPLLAAMPEFCTDNAAMVAGLAGAPGYPAAA
                     AGMDLDVEPNLAL"
     gene            7677..>8824
                     /locus_tag="PKK36_09165"
     CDS             7677..>8824
                     /locus_tag="PKK36_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNR94761.1"
                     /translation="MDLGLLTNFMQMLDDPAIAVDDRLCLLTGNRTACQMLLKDNFIP
                     RSSLRDSLKLDPDAGRRLSDHLAALLRRRGDGPFELTLERGIIFPEPVRITAQTFNIH
                     PRPVSLIIMKTEGARSAPHEELLAGSSIAMIEMSPDQKIVFGGERLAEALHQSCGEVM
                     NAPLSSLVSASVRPLFLAAWTRVMRGEVVNGYEFKLRCGDGHYESCWITLHPVKDHEG
                     NIRSVHGLVGDMRVQKGLAYALEAAEERFSVLFRESSDPILILSLTGDIMLVNPAFEN
                     MAGISAESLFSGKKGWPDFIHEAELPQVMAAIWECSREQKDRTVECRITGGKASGWVE
                     QTFSIIHDENGAARGMLAVGRNITARKEHEIQLQARAELMTQRHGHAQ"
BASE COUNT         1753 a         2706 c         2615 g         1750 t
ORIGIN      
        1 cacgcagcag gtccgtgaga tagaaatact ggccgctggc ctggcgcgtg gccacgatgt
       61 tttccgccag cgtatggctg atgccgccga tacccgacat cagttcctca actgtggcct
      121 gattcaggtc cactcctcgc aggtagagat agagcccctc cttgacctct tctgccgaag
      181 cggcggcagc cggaagagct gactcttccc cggccggggc ttcctcccgg accggttctt
      241 ccgggacggg ttcggcggct tcctcaacgg gagccggctc ttcataataa acggcttcgg
      301 gctcgccttc gacgggcacc gcctcagccg gttcttccgg gacttcgcgg agcagttcct
      361 cttccggcag ttcctcctct gagacggccg cctcttccgc agccgtttcc tctgcggcgg
      421 gggcttccat ttccacgggc gcttcctctt cgggcgcagg cgtctcttcc tccaaaattt
      481 tctggagcat ggcttcaggc gacatttcct cttccgcctc ggcgggctcc ttaacagcga
      541 ccggctcttc ttccggctct gcggccacgg gttcttccgc cacgggctct tcgaccgcct
      601 cttcctctgc aggagcttct tcggccacag gctcctcggc aacagggggt tccccggcgg
      661 gttcttgtgc aaagaccggt tcttcgacag ccggcggctc tgccgcgggt tcttcggggg
      721 ccggctcctc tgcaacaggc tcttcgggca gagtttcctc cacgacagca gcttccggca
      781 cagccgggat ttccggttcc ggagccggct cttcgacggc gggcggaggc gcttccaccg
      841 gcgcaggttc cgcagccgcg cgcatcgcat ccgcggcttt gggtgtcaac tgatccctgc
      901 tgaagacgtt gggcatgccg cgcacgccca gttcgcggtc cctgctggcc gtgcggcgct
      961 tgagttcccc cggctccatg gacgagacca ccagcgggag aggaagcgac atcatgtcct
     1021 ccatatgctg gcggctttcc ggcgtgagat aaccatccgg aatttcgctg atgatatgcc
     1081 gaagcggggt ggtgacacgc ccgctcttga gctgatcaaa aagatcgtcc acaatcacag
     1141 tgaccgtcat ctgcccgctg gccgcgatgt ccgggcgcac aaattccggc gggaggattt
     1201 tcagcacttt gttgagcggg atatccagga aaccgcgttt gtcgctggcg ggagcttccg
     1261 cggccggctc ggccgccccc atgacttcct gaatttcgcg aagctgctcg cgcaggaaat
     1321 caatatcgga cattttggcc ttctcctcga ccggagaagg agcagaaggc ttcaccgccg
     1381 gcggcgcagg cgttttcttg acggggacgg caggcttctt caccggcttc agggttttct
     1441 tgacaggcac tgcgctcttt ttcagaggca tgatcggcct gggcgccgga cccttctggg
     1501 ggggtgtttt gtctttcttc ccgaacaatc cgcctaaaaa tgccacgata aagtcctccg
     1561 cttgaactgc cgcctgttcc accgatggga acactaaagc agatataccc gtaaatgaac
     1621 agcccataaa tcgttttcag catccgattt gagaacgggt gcagtttccg ttgcgcagtt
     1681 ggcggaattg gggtatacat aaagaaaact cttattaagg aataacagcc cagtgtcgtt
     1741 catcaatttt gcacggaaag aaattgcgtt caagatcgtg tactacgggc ccgcgctgtg
     1801 cgggaaaacc acgaatctgg aacagattca tgccgcgatt gtcccggagt cgcggggcga
     1861 aatgaccgtg ctttcaacgc aggccgaccg cacactgttt ttcgatttcc tgcctcttaa
     1921 atccgaaatc atcaaaggct tcacctccaa gtttcagctc tatacggtgc ccggccagac
     1981 catctaccgc gaaacccgca agctggttct gcgcaatgtg gacggcgtcg tctttgttgc
     2041 ggactcgcag tgggaaaaaa tggaggagaa cgctgaaagc ttccaggacc tgattgacaa
     2101 tctggcggag cagagcgtct ccattgacaa aatcccctac atgcttcagt tcaacaagcg
     2161 cgacctgtcc aacgtggccc cggcccatta catggacttc atgctcaatc agcgcgaaac
     2221 ccgtgtgccg accatggagt ccgtggcctg caactgcctc ggggtctttg aaactctgaa
     2281 tatcatggcc aagatgattc tggccgcgtt catgaaggaa aacaacatgg ccatggccga
     2341 tgtgccggac gatgtgtgct tgtagcttaa caggatgaat ggaatgcaaa aattgacgcc
     2401 gcagctgggc aaaaaaatcg acgagattct gctgaatctg gtggtgcgcg ccgaggcgga
     2461 ggccgtgctg ctttgcgacc gcggtgggaa catcgtcgcg cagaacacca ctgagcgcta
     2521 tcatcaggaa gacaacattg ccgcgctggc cgccggttca ttttacgcca cacgcgaact
     2581 ggcccgcctg atcggcgagc cggtcttccg ctacattttt caccgcggcg agaaaaccag
     2641 tctttacatg cagaccacca gcattgacat gctggtggtg gtggtgttcg gaaaggattc
     2701 aaatcccggg ctcgtgaagc tttacggcga cgaggcctgc gcggccctgg aggagctctg
     2761 cgcgcagaac gccgcttcac cggaagaaca cagcgaaact ctcgaactgg actccacgaa
     2821 aaccatgttc aggcggaaat aagccggacg gaattattcg tctgatccaa gcgtgcgcac
     2881 cagcgcccgg aattcatccg tgtttctgat gccgtcaaaa cggggatctg ccagctgtgc
     2941 aagagcattg cgaggatcca tcagggaagc gcccttctga agcgacttga tcgcgtcacc
     3001 ggtgctgccg gtccgataaa gcgcctcacc gagctgcaga taggccgggg ccagctccgg
     3061 ggcctgcttg acaatgcccg tcagcacggg cagcgcccgc tcccacagct gctgctgcaa
     3121 aagcacatgg gcataagcct gactggcctg aaggtctccc ggcttcagct tcaggtaacg
     3181 gtcataatat tccaccgccc tctccggacg gtcggttcgg acatagagtg tgcccagatt
     3241 cagcagcgcc aggctgtagt ccggcttcag ctcaaccgcc cgcagaaagt gctcttcggc
     3301 tttttgataa tccttgagcg tcaggtagga aacgcccaga ttgttttcca gcccgtagcc
     3361 gggagagtcc tgatccgacg ccatcgcgaa ggcctcggcg gccgccgcgg catcgcccag
     3421 ctgcagatag cagaggccgc ggtagtgatg caccccctga atggagggga agatggagat
     3481 gacatggtca tacatggcca gggcctcctc cggattgttg tcctgatagg ccttctccgc
     3541 gcgggtgatg taatatcccg cctgtttgac ccatgtggcc gacaacggga cctcggcgcc
     3601 cgccggcggc agcatcggat tgaagtcggc cacgtcatcc accaccagca gctcttccgg
     3661 cgcggacttc ctgcggccgc cgctcgtcag aatccggtag cctgcggagt ccgaagcctc
     3721 aatgtcaaaa attgagcggg cctgttccgg cggaactctt gacagcatgt aatactgaac
     3781 cgccacgaca ctgatcagca caccgaaagc aacaaccgtc agcagaaaaa gccagttctg
     3841 cagcttcatg cggcgctgtt ccttctgcca ggccgccgct tcggttgcgg caggcggttc
     3901 gcgccatgtc ttttcattgg gggcatggct ggccgctgtc tcgtagatgc tctgtgaaac
     3961 ctttctggtc attcctgttc cgcgctctct tctgttttct gctgggcgga atgtgcatct
     4021 tccagcgcct gatttccagc ttcttttttc aactgcaccg ccgtcaccag atccgcctca
     4081 agctgctgcc cttcggaagt ttttcgagcc gccatgaaaa ccggttcgcc gaaaatcagc
     4141 cgtacctgct cgggcggaat ccgttcggat gcgctgcgca agtaatcaac tgcctttgac
     4201 gtattgccca tcagggcaca ggttgcggcc gcatctgtgt ataacgacgg catgtccgga
     4261 gcgtgctgaa tggcgccggc cagcaggctc atggccgtct cataatttcc cgcggcggct
     4321 tcgagatacg ccaggtatcg cagcgcttcg ggatgaccgg gatgcatttc aagcgcgtcg
     4381 tgaatgtgct tcctggcgcg gtccacatag cccatgacat aatccgcgcg cgccagattc
     4441 aaatggcttt ccacatgttc gggatccagt tcggcggccg tcccaaaaaa tatcgcggcg
     4501 ccgtccggat tcttccgaat cagttcaatg cgtcccaggt cgttcagaac cggggcgcgg
     4561 cccggatcag cggccgccgc gcgctgcaga tgctgccccg ccagcgccca ttctccctgc
     4621 atcagcaggg catggcccag cccggcgtcc gcggcggcat agcccggcca caggtcaagc
     4681 acacgccggt aagccgcagc agcttcgctc caccccccca tcaccgcaag ggcatcggcg
     4741 tcccgcaggg cctgcaccgc ctgaatcatc atttggggat caggcgcagc agcggaaacc
     4801 cacgggcctt ctccaggcag ggaatttttc cgctcctccg cctcatgaag cgccggcgga
     4861 gttcccactc gccgtgatga ggaggttctt cgtgtccgcg tcgtcagttt caggacggcc
     4921 atcaggacca gcagaacaaa aagcatcagg cccgcaaaaa aagccgcccg tcgaaacagc
     4981 ttccagcgcc cctcgtattc cggaggcggc cgcttcaggc ggcagggctc cgcccgccgc
     5041 gtgtcctgat gctgcacgga ataaagatcc cccggcggag tggccggcgg aatctccttt
     5101 gccctgggga cgatttcatc catgcgcgaa acctaccgca ttcacgaacg tttagcaacg
     5161 gcagactccc ccggtgagta tccttgccgg cgccagtcca aaacggcatt tggtttacgt
     5221 tgatattgac gggctcctct cgtatagtga gagggtttgc tgactgcttt cacgtgaata
     5281 cggagatgat ttatggttaa gggattttcc cgacggcacg ccctcggggc cggcacagcc
     5341 ctggtgctgc tcgccaatct ggtgattggc gcccgcatct attcacagga ggctgccgca
     5401 ggaaaagacg atcaggccta cgagaatctg gccctgttca cccgcgttct cgaggaaatc
     5461 cgcatccagt acgtggatga ggataaaacc gaatatcagg atctgatcta cggcgcgctg
     5521 gagggcatgc tggcctcgct ggatgaacac agccagtttc tcgatccgga aatgtacacc
     5581 gagatgaagg aggagacatc cggccagttt ggcggcctcg gcattgtgat cagcctccag
     5641 aacggcatcc tcaccgttgt ctctcccatg gatgacacgc cgggatttcg cgcgggcctg
     5701 acggcgggtg accagatcat cgaaattgac ggtgaaaaca tggagggaag ttccctgcag
     5761 gaggccgtca aaaaactgcg gggaactccg ggcacaaagg tgtcgctgaa gatccatcgt
     5821 ccggaatcac gcgagtatct ggaaatggaa atcgagcgcg ccattatcaa tgttgaaacc
     5881 gtcaaggatg cccgcatgct cgacgggggc atcggctatg tccgcatcac gcagttttcc
     5941 gagcccacgg ccggcgccct gcagaaggcc ctggacgaac tgttcgaaca gggaatgcag
     6001 gcgctggtgc tcgatctgcg ctacaatccc ggaggactgc tgacctccgc agtggaagtg
     6061 agccagaagt tcctgaagaa gaacaaggtc attgtttcaa cccgcggacg cgatgaaaag
     6121 gaggctcagg ttttccgggc gcgaggccgg gaacattatg cagacctgcc gctcgccgtg
     6181 ctggtgaatt ccggcagcgc cagcgcttcc gaaattgtca gcggggccct gcaggaccac
     6241 aaccgcgcca ttatcatcgg cgaaaaaact tttggaaaag gttccgtgca gagtgtgctc
     6301 ccgcagcagg acggctcggc catccggctg accacatcaa aatattatac cccggacggg
     6361 cgggtcattc acgaaaaggg cattgaaccg gatatccggg tgtccatgcc ccacgaggtg
     6421 tggcagaagc tgatgctccg gaagcagagc actgagcaaa ccggagggga agacgtgaag
     6481 gacgtgcagc tcgaacgcgc ggtggatgtc cttcgcggca tccgcatctt ccaaacccag
     6541 aatcgaaaag gcattttcac ggctcgctcg ggcggatgaa accatgatcg tgcttggcat
     6601 agaaacctcc tgcgatgaga ccgctgtcgc actggtcgaa aatggactca agatccatgc
     6661 tcactccgtc tacacccaga tcgccgcgca ccgcccgtac ggcggggttg tcccggaaat
     6721 cgcctgccgc agccatgtgg aaattcttcc cggcctcatc cgggacaccg ttgccgattc
     6781 ggggcttgcg tgggaccgga tcgatgccgt ggctgtcacc tatggtccgg ggctggccag
     6841 ttcgcttctg gtcggggtgg cggcaggaaa ggctctcgca ctgcgcctgg aaaagccgct
     6901 gatcgccgtc aaccacctgc gggcccacat ctattcactg ttcatggggc gcgaggatct
     6961 gagcccgaaa caggtttatc ccctgctcat cctgctggtc tcaggaggcc acacctgcct
     7021 ggtgcgggca gacagcctca atgaatgtca tctgctggga caaacgcttg acgatgcggc
     7081 cggcgaggcg ctggacaagg gcgccagcgt gctgggtctg ggatatccgg gcggaccggc
     7141 catcgaaaaa gccgcctggg acggcgaccc cggattcgtc cgctttccgc gcggcctgcg
     7201 gcatggcgcc cagagcagcc tggccggatc cctcgaaaga gatctgtgct tcagcttcag
     7261 cggcctgaag acctcgctgc gctactatgt gcatgatcac cccgatgtgg tcgcgggtga
     7321 gaatctgaag aacgtggcgg cgagctatca ggccgcggtc attgacgccc ttgtggaacg
     7381 gctcgagagg gcgctggccc ggcacgacgt gaaggcggtc gcctgcgtcg ggggcgtggc
     7441 gcgcaatcag gtgctccgcg acaaactgac cgaactcgcc gaagcccgca agctgccgct
     7501 cctggcggcc atgcccgaat tctgcaccga caacgccgcc atggtggccg ggctggcagg
     7561 agcgcccggc tatccggcag ccgccgcagg aatggatctg gatgtggaac ccaaccttgc
     7621 actctagcct gaaaactgtt tgaatgctgc gcgggactgg gcgggggaat gaacatatgg
     7681 atttgggact gctgacaaat tttatgcaga tgctggatga tccggccata gccgtggatg
     7741 accgtctctg ccttctgacc ggaaacagaa ccgcatgcca gatgcttctg aaggacaact
     7801 tcattccccg gagctccctg cgggattcgc tgaaactcga tccggatgcg ggccggaggc
     7861 tttccgatca tctggcggcc ctcctgcggc gcagagggga cggcccgttt gaactgacgc
     7921 tggaacgcgg catcatcttc cccgaacccg taagaatcac ggctcagact ttcaatatcc
     7981 acccccgccc cgtgtccctg attatcatga agacagaggg ggcgcgttcc gcaccgcacg
     8041 aggaactgct ggccggctcc tccatcgcga tgatcgaaat gtcccccgac cagaaaattg
     8101 tctttggcgg cgaacggctg gcagaggccc tgcaccagtc ctgcggcgag gtgatgaatg
     8161 ccccgctttc ttccctggtg tccgccagcg tgcgcccgct cttcctcgcg gcatggaccc
     8221 gcgtgatgcg cggcgaggtg gtgaacggct atgagttcaa actccgctgc ggcgacggtc
     8281 attacgaatc gtgctggatc accctgcacc cggtgaagga ccatgaggga aacatccgca
     8341 gcgtgcatgg gctggtgggc gacatgcgcg ttcagaaggg tctggcctat gccctcgagg
     8401 ccgccgagga gcggttcagc gtcctgttcc gtgaatcctc cgatcccatc ctcattttgt
     8461 cgctgaccgg cgatatcatg ctggtgaatc cggcttttga aaacatggcg gggatttccg
     8521 cagaaagcct tttttccggg aagaaaggct ggcccgactt tattcacgag gcggaactgc
     8581 cgcaggtcat ggcggccata tgggaatgtt cacgggaaca gaaagaccgc accgtggaat
     8641 gccgcatcac gggcggaaaa gcctccggct gggtggagca gaccttcagc atcatccacg
     8701 acgaaaacgg cgccgcgcgc ggcatgctgg cggtcggccg caacataacg gcccgcaaag
     8761 agcatgagat ccagctgcag gcacgggccg aactgatgac ccagcgccac ggccacgccc
     8821 agga
//
LOCUS       DAOQWR010000117         8356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_8997_length_8356_cov_4.898928, whole genome shotgun sequence.
ACCESSION   DAOQWR010000117 DAOQWR010000000
VERSION     DAOQWR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8997_length_8356_cov_4.898928"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKK36_09170"
     CDS             complement(<1..771)
                     /locus_tag="PKK36_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR94762.1"
                     /translation="MLKKLMIGMLMAGLLAAPGMLVCSAPAQEADAEALEIAPGMEVD
                     AAAATGSKSSSGFAQVLFGSGAFGFMLWMLLAACSVAGVALIVDSFINIREKKIAPEV
                     LAEDVKTAMGQGDVMKALKHCEDAPGPLANILSAGFSNVEEGFEAIQDSVGVAADLES
                     EKLLQRVAYLNLVGNLAPMLGLLGTVQGMIMAFATLATQSAGAAQQSALAMNISQALY
                     TTAAGLLVAVPAVAFFYFFKNRATRIILSMEGMTMDLIK"
     gene            complement(857..1660)
                     /locus_tag="PKK36_09175"
     CDS             complement(857..1660)
                     /locus_tag="PKK36_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94763.1"
                     /translation="MKQNLNTKTGKILAGALAAFLLLGICATDAAYVILQGNRRIEGT
                     NLRMRPNGDVTLVTANGPVTFTKAQYLTAMADEPPEYNQGRQLAAAGKVDEAIKLFEG
                     IVSGYKGLTWDAKAIAELSPLYLQKGEAAKAVSMYESLFSISEQSKQDASVQWAYRDA
                     LLKAERYAKLEQDLNSLISTGSREDAARAQIMRGDMNAAQGRTEPAALDYLRTVVLYK
                     AVKDLQPEALFKAAGALEALRDARARELYAQIVRDYPQSPYAARAASKK"
     gene            complement(1691..4501)
                     /locus_tag="PKK36_09180"
     CDS             complement(1691..4501)
                     /locus_tag="PKK36_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94764.1"
                     /translation="MTKYFTAANRHTAIPAGLLAFLLLAAPAPAADIMVDREFEFASG
                     LIEMGYADFAEKAMQQLLLKHPDQKERAKLITAQVMISKRRYQDAEELIATFAAGDPK
                     ADAIRLALANGYFAVEEMEKAKALYDAFFKQYGGRVPTDPDLLRFYQDAAYKYGQMQE
                     KVGDVQGAVQAYERVLATRPGGDVERFLQSHQAALYMRQAETSDGDTRTKALNKAAEL
                     CTQIQWGPLDLAFGNSIVTLARIERLRGKEDAAEEVLKTNMDILKEIDKFMKGEGLPM
                     KESPMAGARFMLGDVAEKKGEGLAKDQAKRNDAIAAYGKALSEYFNVFAKYGESEWGP
                     EAGARAKKIKALLESWGKTVNIDLGEHEREAAKSQLKLADGYFRDAAHDKAIAEYLKV
                     LNDYPETELSQAALGSLAQSYAAKDDILMVKMVSGYLAERFAGKPDAAVALLRLGKLY
                     LDKQDAPMYEQIYGDYVRNFPKDTRAPGVLFAMAQLRKKAGDEAGANTFYQQIIEKYP
                     GDVNFIKALGQIAWGHYLGKNYAEAVPALARYVKALQPGFEKAQAQFCIADSHKQLNQ
                     YPEAIEAYKTVMGWLSPKEKNPYNATASDVSKNRDLLEKSVFYLGYVYTRMNEPKEQI
                     PAFREQARKSYEYFLQAFPQSTLAPAAMSGLGALQLDRGQYDQAAATFEKLAATYPDS
                     AEGKSANFSLIRSAMEVGKNDIAQSAFEKMLQNKDAFSPAEFNRVGQLMLDNGLYEPS
                     IMAFRQVRGATDDRAHLERALFGLGSAHYEMKNYDESIKPLEELMERYPQSGLFYDAK
                     FILGNNYRATGRPKEATDVMKDIFKYADKPDLVARANYTLGLIQIEGKEPDAALASFQ
                     RVALLSDAKDPVLRPIVEKCILESIKVAQELERWKDAADSVEQFLEQFPESPDVEKIR
                     ALRADLRLKASAAAPASAAPAQ"
     gene            4701..5783
                     /locus_tag="PKK36_09185"
     CDS             4701..5783
                     /locus_tag="PKK36_09185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNR94765.1"
                     /translation="MKDSRVQNLAKILVHYSVRAKKGQTVEVSGAAGAEPLIVAVYEE
                     ILKAGAFPVVTMTPDCLSEIFYKKGKPHHFAQYTRWQKSSIMNLDSTIRISCSDNTRA
                     LSSVDPKKQAQHQKTMKPLKRILLAKPWVLTLFPTQAFAQDADMSLTEFEDFVYSATF
                     ADKKDPIGEWKALEKRQAGIIRKLRGADRIRIVGDETDLTFSVKGRTFINSHGTHNMP
                     CGEVFTGPVEDSAEGHIKYDYPVCHAGREVDGIRLVFKKGLVVEATATKNQKFLTDML
                     NMDPGARRLGELGIGTNYGIQRFIKNILFDEKIGGSVHLALGESYPETGGRNKSALHW
                     DMIKDLRKGGAFYVNGKLFQKNGKFV"
     gene            5964..6884
                     /locus_tag="PKK36_09190"
     CDS             5964..6884
                     /locus_tag="PKK36_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNR94766.1"
                     /translation="MNKEHNLIEKYFALVILLVAVLALWLPRCFVWAKPHIPLLLGII
                     MFGMGVTLKFSDFIAVWRHRGMVLAGVALQYTVMPALAVLISSALGLPKEVMIGMVIV
                     GSCPGGTASNVLCYLSRADVALSVVLTLCSTLLAPVVTPFIIYLILSQKVDVSFVAMM
                     QSIFWIVFFPLLAGLVVRHFFFNRIAKVLSWFPTLSILMIAVVIGCVMALNRATLLAF
                     PALVLLAVILHNTLGLAAGYAAGRLLKASPERTRTLAFEIGMQNSGLGVSLATQFFTA
                     AAALPGAIFSVVHNLTGVLMAKWWSVRKAP"
     gene            6965..7108
                     /locus_tag="PKK36_09195"
     CDS             6965..7108
                     /locus_tag="PKK36_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94767.1"
                     /translation="MAKKNCSERDSYAKRFEYMNMGDKIEHVCIIALIALLAVTLAVA
                     LMV"
     gene            7402..>8356
                     /locus_tag="PKK36_09200"
     CDS             7402..>8356
                     /locus_tag="PKK36_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR94768.1"
                     /translation="MKKRNKRKIKTTVHAGINIREVRPDYFLVDCQRGLNRERKCFKD
                     LAEAKTYCDILATKIRNEGTDALGLPSDSRADATKALEILDGKTSLVAAARFWRRHNA
                     VADGMTVSDLGDKWFAALRRSGCRETTMREREHKVGRLAAAFGDRPAAGIMSDDLVQL
                     MDTLHVTGVTADGYRRCWHALYTFAISEGIAEVNPVAKIKPFKSDEKLPKPFAVKDVR
                     SILATAAEYAPALVPTLAVQFFGGLRPGEALGLTWENIDLDEKTIRVLPEVSKMRRSR
                     IVDINDTLAAWLRRYRKKSGPIGIVTHGQFAHFMHRKPIGPE"
BASE COUNT         1566 a         2546 c         2447 g         1797 t
ORIGIN      
        1 cttgatcagg tccatggtca tgccttccat cgaaaggatg atgcgcgtgg cgcggttctt
       61 gaagaaatag aagaaggcca cggcgggcac ggccaccagc agcccggcgg ccgtggtgta
      121 aagggcctgc gaaatgttca tggccagggc ggactgctgc gcggcgcccg cgctctgcgt
      181 ggccagcgtg gcgaacgcca tgatcatgcc ctgcactgtt ccgagaaggc cgagcatcgg
      241 cgcgagattg cccaccaggt tcagataggc cacgcgctga agcagctttt cgctctccag
      301 gtcggcggcg acgcccacgg agtcctgaat ggcctcaaag ccttcttcca cattggagaa
      361 accggcgctg agaatattgg ccagcgggcc gggcgcatcc tcgcagtgct tgagcgcctt
      421 catcacatcg ccctgcccca tcgccgtctt gacgtcttcg gccaggactt ccggggcgat
      481 cttcttctcg cgaatgttga tgaacgaatc cacaatcagc gcgacgccgg cgaccgagca
      541 ggcggccagc agcatccaca gcatgaagcc gaatgcgccg ctgccgaaga gcacctgcgc
      601 aaatcccgaa ctcgactttg aaccggtcgc cgcggcggca tccacttcca tgccgggcgc
      661 gatttccaag gcctccgcat ccgcctcctg cgccggggca ctgcacacaa gcattcctgg
      721 ggcggccagc aggcccgcca tcagcattcc aatcatcagt ttcttcagca tggttgtctc
      781 ctttgtcttg aaccggttca aattgccttt actgctttca cactacttat gtttcagaaa
      841 aatgtctctc ccaatttcac tttttcgacg cggcccgggc cgcatagggg ctctggggat
      901 agtcgcgcac gatctgcgcg tacagctccc gcgcgcgcgc gtcccgcagc gcttccagcg
      961 cgccggcggc cttgaacagc gcctccggct gcagatcctt cacggctttg tagagcacca
     1021 ccgtgcggag ataatcgagg gccgccggct cggtccggcc ctgcgcggcg ttcatgtctc
     1081 cgcgcatgat ctgggcgcgc gcggcgtcct cgcggcttcc ggttgagatc aggctgttca
     1141 ggtcctgctc cagtttggcg tagcgctcgg ccttcagcag cgcgtcgcga taggcccact
     1201 gaacagacgc atcctgcttg ctctgttcgg aaatggaaaa caggctttca tacatcgaaa
     1261 cggccttggc ggcctcgccc ttctgaagat aaagaggaga aagctccgca atggccttgg
     1321 catcccaggt caggcccttg taaccggaca caatgccctc gaacagcttg atggcctcgt
     1381 ccaccttccc ggcggccgcc agctggcggc cctgattgta ttccggcggc tcatcggcca
     1441 tcgccgtcag atactgggct ttggtgaacg tcacgggccc gttggccgta accagcgtga
     1501 catcgccgtt cggcctcatg cgcaggttgg tgccctcgat ccgccggttg ccctgcagta
     1561 tcacataggc cgcgtccgtc gcgcagatgc cgagcagcag gaaggccgcc agcgctcctg
     1621 caagaatttt ccccgtcttc gtgttcaggt tctgtttcat gattaacctc tcttcattaa
     1681 taatcagcaa tcattgtgcc ggtgcggcgg acgccggagc cgcggcgctg gctttcagcc
     1741 gaagatcggc gcgcagcgcg cgaatcttct ccacatccgg gctttccggg aactgttcca
     1801 ggaactgctc cacgctgtcc gcggcatcct tccagcgctc cagctcctgg gccaccttga
     1861 tggactccag aatgcatttc tcgacaatgg gacggagcac ggggtccttc gcgtcgctca
     1921 gcagcgccac gcgctggaac gaggccaggg ccgcgtcagg ctccttgccc tcgatctgaa
     1981 tcagtcccag cgtgtagttt gcgcgggcca ccaggtccgg cttgtccgca tatttgaaga
     2041 tgtccttcat gacatcggtc gcctctttgg ggcggccggt cgcgcgatag ttgttcccga
     2101 ggatgaactt ggcatcatag aacagtcccg actgcggata gcgctccatc agctcctcca
     2161 ggggcttgat ggactcgtcg tagttcttca tctcatagtg cgcggagccc agcccgaaga
     2221 gggcgcgctc cagatgcgcg cggtcatcgg tcgctccgcg aacctgccgg aacgccatga
     2281 tcgaaggctc atacaggccg ttgtccagca tgagctgacc cacccggttg aactccgccg
     2341 gggaaaaagc gtccttgttc tgcagcattt tctcgaaggc cgactgcgca atgtcgttct
     2401 tgcccacctc catggccgag cggatcagcg agaagtttgc gctcttgccc tcagcagagt
     2461 ccggataggt cgcggccagc ttttcaaagg tcgccgctgc ctggtcatac tgtccgcggt
     2521 cgagctgcag cgccccgagg ccgctcattg cggcaggcgc cagcgtggac tgcgggaagg
     2581 cctgcaggaa gtactcatag cttttgcgcg cctgctcccg gaaggccgga atctgctctt
     2641 tcggctcgtt catgcgggta tagacatagc ccagatagaa gaccgacttc tccagcagat
     2701 ccctgttctt cgacacatcc gaagccgtcg cgttgtaggg gttcttttcc ttcggggaga
     2761 gccagcccat cacggttttg taggcctcaa tcgcctcggg atactggttg agctgcttgt
     2821 gggaatccgc gatgcagaac tgcgcctggg ccttttcaaa tcccggctgg agggccttca
     2881 catacctggc cagggccggg accgcctccg cataattttt gccgaggtaa tgcccccatg
     2941 cgatctgtcc cagcgccttg atgaagttca catccccggg atatttctcg atgatctgct
     3001 gatagaacgt gttggcgccg gcctcgtcgc cggccttctt ccggagctgg gccatggcaa
     3061 agagcacgcc cggcgcgcgg gtgtccttcg ggaaattccg gacataatcg ccgtagatct
     3121 gctcgtacat cggcgcgtcc tgcttgtcca ggtaaagctt gcccagcctc agcagcgcca
     3181 cggcggcgtc cggtttgccc gcaaaccgct cggccaggta accgctcacc attttgacca
     3241 tcaggatatc gtcttttgcg gcatagctct gcgcaaggct gcccagcgcg gcctgcgaca
     3301 gctcggtttc cggatagtca ttcaagacct tcaggtattc ggcaatggcc ttgtcgtgcg
     3361 cggcgtcgcg gaaatagccg tccgccagct tgagctggga cttcgcggcc tcgcgctcgt
     3421 gttcacccag gtcaatgttc acggttttgc cccacgattc gagcagggcc ttgatctttt
     3481 tcgcgcgcgc gcccgcctcg ggcccccact cgctttcccc gtacttggca aagacattga
     3541 aatattcgct gagagccttg ccgtaggccg cgattgcatc attgcgtttg gcctgatcct
     3601 tcgcgaggcc ctcgcccttt ttctcggcca catccccgag catgaagcgc gcgcccgcca
     3661 tcgggctctc cttcatcggg aggccctcgc ccttcatgaa cttgtcgatc tccttcagga
     3721 tgtccatgtt ggtcttgagc acttcctccg cggcgtcctc cttgccgcgc aggcgttcaa
     3781 tgcgcgcgag ggtgacaatg gaatttccga acgcaaggtc cagcgggccc cactgaatct
     3841 gcgtgcagag ttcggcggcc ttgttcaggg ctttggtgcg ggtgtcgccg tccgaagtct
     3901 cggcctgcct catgtagagc gcggcctgat gggactgcag gaagcgctcg acatcgccgc
     3961 cgggcctggt ggccagcacc cgttcatagg cctgcaccgc gccctggacg tcgccgacct
     4021 tctcctgcat ctggccgtac ttgtaggcgg cgtcctgata gaagcggagc agatccggat
     4081 cggtgggcac gcggccgccg tactgcttga agaacgcgtc gtagagcgcc ttggccttct
     4141 ccatctcctc gaccgcgaaa tagccgttgg ccagcgccag ccgaatggca tcggccttcg
     4201 gatcgccggc cgcgaacgtg gcaatcagct cctcggcatc ctgatagcgg cgcttcgaga
     4261 tcatcacctg cgcggtgatc agcttggcgc gctccttctg gtcgggatgc ttcagcagaa
     4321 gctgctgcat cgccttctcc gcgaagtcgg catagcccat ttcgatcagg ccggaggcga
     4381 actcgaactc gcggtccacc atgatgtcgg cggcgggcgc aggcgcggcc agaagcagga
     4441 aggccagcag cccggcgggg attgcagtgt gacgatttgc ggcagtgaag tatttggtca
     4501 taaatctctt tcgtaacctg acatatttca ttccaccaac ctcgttgtct ctggaagtga
     4561 acattaaaca aaatgaactg tgtgcgtcaa gattatcgct gatattcgga tcgcgctccc
     4621 gcgcggaggc ctggccgtgc tattgacacg gcatggaaag atcagcagga taggccgcca
     4681 gtttcacagg agaagttttc atgaaagaca gtcgagttca gaatctggcg aaaattcttg
     4741 tccactattc ggtcagggca aagaagggac agaccgtgga ggtctccggc gcggccggcg
     4801 cggaaccgct cattgtcgcg gtctatgagg aaatcctgaa ggccggcgcg tttccagtcg
     4861 tgaccatgac gcccgactgc ctcagcgaga ttttctacaa aaagggaaag ccgcaccact
     4921 tcgcgcagta caccagatgg cagaagtcct caatcatgaa cctggactcc accatccgga
     4981 tttcctgcag cgacaacacg cgcgcgctgt ccagtgtgga cccgaagaag caggcgcagc
     5041 accagaaaac catgaagccc ctgaagcgca tcctgctggc caagccgtgg gtgctgaccc
     5101 tctttcccac gcaggccttc gcgcaggacg cggacatgag cctgactgaa ttcgaagatt
     5161 tcgtctacag cgcaaccttc gccgacaaaa aagatcccat cggcgaatgg aaagcccttg
     5221 aaaagcggca ggccggaatc atccgcaaac tgcgcggcgc ggaccggatc cggatcgtcg
     5281 gcgacgaaac cgatctcacc ttctccgtca agggccgcac gttcatcaac tcgcacggca
     5341 cgcacaacat gccctgcggc gaggtgttca ccgggccggt tgaggattcc gccgaagggc
     5401 acattaaata cgactatccc gtctgccatg cgggccggga agtggacggc atccggctgg
     5461 tcttcaaaaa aggcctcgtg gtcgaggcca ccgccaccaa gaaccagaaa ttcctgaccg
     5521 acatgctcaa catggatccc ggcgcgcgcc gactcggcga gctgggcatc ggcaccaact
     5581 acggcattca gcgctttatc aagaacatcc tgttcgacga aaaaatcggc ggctcggtcc
     5641 atctggcgct gggcgagtcc tatcccgaga ccggcggcag aaacaaatcc gccctgcact
     5701 gggacatgat caaggacctc cgcaagggcg gcgccttcta cgtcaacggc aagctgttcc
     5761 agaaaaacgg aaaattcgtg tgaaactgaa tatagtacta atttagtact atttagcatt
     5821 tttatcgctt ttttggactt ttttgcgttt tttgacgatt tttagccttt ttggggccgt
     5881 ttttttgcgt ttttgggccg tttttagcga tttttcggcg tttttaacgt ttttgttgat
     5941 ttttatgcga tgcgcgcgcg gacatgaata aagagcacaa cctgattgag aaatattttg
     6001 cgctggtgat tctgctggtg gcggtgctgg cgctgtggct cccgcggtgc tttgtgtggg
     6061 cgaagccgca tattccgctc ctgctgggca ttatcatgtt cgggatgggc gtcacgctga
     6121 agttttccga tttcatcgcg gtctggcggc accgcgggat ggtgctggca ggcgtggcac
     6181 tgcagtacac cgtcatgccg gcgctggcag tgctgatttc ctcggcgctg ggcctgccga
     6241 aggaggtcat gatcggcatg gtcattgtcg gctcctgccc cggcggcacg gcctccaatg
     6301 tgctgtgcta tctttcgcgc gcggatgtcg cgctgtcggt tgtgctcacg ctctgctcca
     6361 cgctgctggc cccggtcgtg acgccgttca ttatttacct tatcctctcg cagaaagttg
     6421 acgtgtcgtt tgtcgcgatg atgcagtcca ttttctggat tgttttcttc ccgctgctgg
     6481 cggggctggt ggtccggcac ttcttcttca accggattgc aaaagtgctg tcgtggtttc
     6541 cgaccctttc catcctgatg atcgcggtcg tgatcggctg cgtgatggcg ctcaaccgcg
     6601 ccacgcttct cgccttcccc gcgctggtgc ttctggccgt gatcctgcac aacacgctgg
     6661 gcctcgcggc gggctatgcg gccggaagac tgctgaaggc ttccccggag aggaccagaa
     6721 cgctggcgtt tgaaatcggg atgcagaatt cgggactggg cgtgagcctt gcaacacagt
     6781 tcttcaccgc ggccgcggcc ctgcccggcg ccattttcag cgtcgtccac aacctcaccg
     6841 gcgtcctcat ggccaaatgg tggagtgtcc ggaaggcccc ctgatcgctc cgccccgcat
     6901 ttttttcttg cgtgcaccgc atttgttttc ctaaattccc ccacgattcg caatggggga
     6961 atcaatggcg aaaaagaact gcagcgagcg cgacagctat gccaagcgct tcgaatacat
     7021 gaacatgggc gacaagattg agcacgtgtg catcatcgcg ctcatcgccc ttcttgccgt
     7081 caccctcgcc gtcgcgctga tggtgtagcc cgcaggtcgt gacagtactt ccctcccgcc
     7141 tgcccgatcc gctgtgcgta tcgggctgcg cagggcatac ttcgctcttc gctgcggcat
     7201 aagaagcgtg gcttgccata cgaagctctg agcgcagcga agagcgaagt atggtcgggg
     7261 cggtgggatt tgaacccacg acttacagct cccaaagctg tcattttttg acttttcatt
     7321 ggcttttttg agggctgttg acattttgtt gacatttcgc ctctttagac atacctttgc
     7381 cagcatatgg aggaccatgc catgaaaaag agaaacaagc ggaaaattaa aacgacggtg
     7441 catgcgggaa taaatatccg cgaagtgagg ccggactatt ttctggtgga ctgccagcgg
     7501 ggactgaacc gggagaggaa atgctttaaa gacctcgcgg aggctaaaac atattgcgat
     7561 attctcgcga caaaaatccg caatgagggg acggatgcgc tgggtctccc gtcggactcg
     7621 cgggccgatg caacaaaggc gctcgagatc ctcgacggca agaccagcct agtcgccgcg
     7681 gcgcggttct ggaggcgaca caatgccgtc gcggacggga tgaccgtctc ggatctgggc
     7741 gacaagtggt tcgcggccct ccggcggagc ggatgcagag agacgaccat gcgcgagcgc
     7801 gagcataagg tcgggcggct ggcggcggca ttcggggacc gccctgctgc cgggatcatg
     7861 tcggatgatc tggtccagct tatggacacg ctgcacgtca ccggagtcac ggcggacgga
     7921 tatcgccgct gctggcatgc cctctacacg tttgctatat cggagggaat agcagaggtt
     7981 aatccggttg ccaaaatcaa accattcaaa tcggacgaga aactcccgaa accgtttgcg
     8041 gtgaaggatg tccggtcgat actagcgacg gcggcagaat atgcgcccgc gctggtcccg
     8101 acgctggcgg tccagttttt cggggggctt cgccccggag aggcgctggg tctcacttgg
     8161 gagaatatag acctcgacga aaaaacaatc cgcgtcctgc cggaggtctc aaaaatgcgc
     8221 cgctcgcgta tcgtagacat aaacgacact ttggccgcat ggttgcggag ataccggaaa
     8281 aaatccggcc ctatcgggat cgtgactcac gggcagtttg ctcactttat gcaccggaaa
     8341 cctatcggtc cagagt
//
LOCUS       DAOQWR010000118         8301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9079_length_8301_cov_4.802450, whole genome shotgun sequence.
ACCESSION   DAOQWR010000118 DAOQWR010000000
VERSION     DAOQWR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9079_length_8301_cov_4.802450"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PKK36_09205"
     CDS             complement(<1..1323)
                     /locus_tag="PKK36_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94769.1"
                     /translation="MKRIILVLAAILTAMSAPADPVAEIDYQGKVLVGDAAFTGTGYF
                     KLALADADSATTFWSNDGTLAGEPAGVLTGEVYSGVFSLTIGDAAVMEALDPDLFSAG
                     EDLYLRVWFSTNAAAGFEEMLPSQKVLSSPYAINAARVSGLDAAGIRAGLATGTPVYA
                     ESDPVWNSQKAGYATGTPLYVESPETDPVWSAASNLYYLQSEADALFSTGTPVYVEED
                     PNYADDLAGGVIATGTPVYAETDPVWAGASNLYYLQTAANARFATGMPVYVETDPVFL
                     ARSNSIVFDGDAARGDLGGTYPSPTVTGLQGRAVASSAPSDGDVLQWNGTAWAPAEVS
                     SGTGGTSGAAGGDLTGTYPNPTIAKLQGATLFGMSGLGPFDTNRYVGWNGVRWEPRLV
                     TPAESDPYFSAVSNKLILTNTTAGGDLAGTYPNPTVDGLAGKSIVVSSL"
     gene            1463..2827
                     /gene="mnmE"
                     /locus_tag="PKK36_09210"
     CDS             1463..2827
                     /gene="mnmE"
                     /locus_tag="PKK36_09210"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNR94770.1"
                     /translation="MQAPDDTIAAIGTAPGTGGIAVVRVSGPRTMEIADRIVRCAAPR
                     PSMRRTMSVIYGHIAAAAGDVDEVLVLVMKGPGSYTREDMIEIQGHGGRVMASAVLQT
                     VLDAGARLAEPGEFTKRAFLNGRIDLTQAEAVADIVGARSRRAGRAALEQLEGSLSDA
                     VNALYDRLMGIAADMEATLDFPEEDIPEALIQDVPDRISRAVSEMDLLISSWQEGRLL
                     REGALIVISGRTNVGKSTLLNALLDTDRAIVSHIPGTTRDIIEEGLVLDGIPVRLVDT
                     AGLRETSCEIESEGIRRTMRHIARADLQLHVLDSSQESGNEFDAQISGLDPIRTILVL
                     NKTDLGVRICRDNLNGFTCVETSLLNRTGLEALKKEIRRALMQDIKMDAPPHAVISER
                     HRQLLLKAREDALGADSLFGQDDIAMASSRLRESIEHVGLITGRVYQEDLLNAVFSKF
                     CIGK"
     gene            2845..4821
                     /locus_tag="PKK36_09215"
     CDS             2845..4821
                     /locus_tag="PKK36_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNR94771.1"
                     /translation="MNFSYDILVAGGGHAGCEAALAAARMGARTALLTINRTTIARMS
                     CNPSIGGIAKSHLVFELDALGGEIARNTDFTGIQFRKLNTRKGPAVQAHRVQCDKRLY
                     PLRMLAVMRRTANLDILEGMAAEVWIETGRLRGVTTDDGSKIGARAVILTPGTFLNGV
                     MHIGMNRIPGGRTDEAPANRLSESLKSLGFLLGRLKTGTPPRLHKDSIDYSKCQIQPG
                     EVPPPFLSRAAARIWKMFHVEQSEVSGSFLDELFHVEQFDNRDAINPSFQCSTARSSS
                     ALLPWPPGRDQLPCYLTHTTSQTHDIIRANLEKSALYGGAITGTGVRYCPSIEDKIVK
                     FPQRESHHVFLEPEGRDNTRVYPNGTSNSLPEDVQIQMIRSIPGLEKAEFIRPGYGIE
                     YDYSDPTQLYHSLETKLVEHLYFAGQINGTTGYEEAAAQGFAAGVNAVLRLRGDAPFV
                     PLRNEAYIGVMLDDLVTKGVDEPYRMFTSRAEHRLLLRQSNAPFRLADHAARIGILSK
                     TEVASVRAQRDLIEGEVRRLNESFLDNQSLASILARQDTTYADLLGALSGLDPLIINE
                     IETDIKYAGYIRREQQKIEKLLKLEHQPIPHDFDYDAIRALRFESREKLKKIRPENLG
                     QASRISGVNPADISILSIWLKRSRSQNACTADGR"
     gene            complement(5087..8074)
                     /locus_tag="PKK36_09220"
     CDS             complement(5087..8074)
                     /locus_tag="PKK36_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR94772.1"
                     /translation="MKPKSHSEKPRWRPLFMATLFCLAGQTALSAYSLVMTNGYGDVY
                     RMNLLRDRYNYQAYRGVCSYRGFSLSGCSTTGEVVMLRIPATAAMPEHFSILSTSKRW
                     DTTMYVLNDVFRWINSTTMAGYENWYPFDMSCSVAMYINSGGFGSASLAKAPEKRVGP
                     LRTESEDPVSDPRFIFSLLDEPAPAPRLGAKAYSLVMSNGYGDVYRMNLLRDRASYQA
                     YRGVCSYRGFSLSGCSTTGEVVMLRILATATMPEHFSLVSTSLRWGTTMYFLNDVFRW
                     INSTTMAGYENWYPFDMSCSVAMYISSGGLGPAGAEKSGPRTIRMPGPKLAPGGTNVI
                     GALIPLTGDLTDLGASYQAAYEIALAEVTNTPGMPPIRLEIRDTVTDPLVAVEQLQQL
                     YTQDVQVVLGPETSAECEGLLEFANDHGMLLLSSSSTALPLAVPDDNLMRLPVSDDRQ
                     AEALAEDIIYDGITNLLLLTRSDMYGEGLRSRLLQEFEARGGQVFYTGYCPRATGFIP
                     EVVTQLNAIVSARAADVGAENIGIVLAVFDEGVQILEQAAAFPTLAAARWYGSEGMAG
                     NAALLTNAAARTLAMDTQFTCTEPDEYTNALYSAVSDAIEARTGSRPRPYAMHAYDAL
                     WLAALALRDTGGTGTVQEVRAAIRSEAAGYDGASGPVLFNAADDRSDGLFQCVRVTDS
                     NEWTNILQTIPEPPDTRAASGLAVNSFRAQWGSSAGALEYFLDVSAGAGFDPADYVAT
                     FSNQPLGAVHSHAVTGLTPGETYYYRVRAGNAEGVSPNSSVIPVTLPTSSVIQASANA
                     GGWLVPSGAVNVEPMASVDFTVTASNYYEIAEIRTNGSPLAGVDGLTCYTHRWETVTS
                     DGEFVAVFRALTAVNDVPLWWLAQYYTVTTNGDELSVSDSDVDGFPAWQEYIAGTVPT
                     NFESRLCFKLIEQASGSGLVPRWNSATDRLYTLYYSADPAEGIWTCEPTCVDVPGTGG
                     ELCFTNSLPQVAGFYRLGVCVEP"
BASE COUNT         1748 a         2479 c         2456 g         1618 t
ORIGIN      
        1 cagcgaactg accacaatgg acttgccggc aagcccgtcc accgtcggat tgggataggt
       61 tcccgccaga tcgccgccgg ccgttgtgtt cgtcaggatc agcttgttgc tgaccgcgga
      121 gaaataggga tccgattcag ccggtgtcac aaggcgcggc tcccatctca cgccgttcca
      181 cccgacgtac cggttggtgt caaagggccc caggcctgac atgccgaaaa gcgtcgcgcc
      241 ctgaagtttg gcgatggtgg gattaggata cgtgcccgtc aaatccccgc cggccgcacc
      301 gctcgtgccg cccgttcccg aggacacctc ggccggtgcc catgcggttc cgttccactg
      361 aagcacatcg ccgtccgatg gcgcggacga cgccactgcc cggccctgca aaccggtcac
      421 ggtcggactg ggataggttc cgcccagatc tccgcgcgct gcatcgccgt cgaatacaat
      481 gctgttgctg cgcgcaagaa aaacgggatc ggtttccaca tataccggca tgccggtggc
      541 gaaacgcgca ttggccgcag tctgcaggta atacagattg ctggccccgg cccagacggg
      601 atctgtttcc gcatacaccg gcgtgcctgt cgcgatcacg ccgccggcca ggtcgtcggc
      661 atagtttggg tcttcctcca catataccgg agtgccggtc gaaaacagcg cgtcagcctc
      721 ggattgaaga taatacagat tgctggcggc gctccagacc ggatcggtct cggggctctc
      781 gacataaagc ggcgtgccgg tggcatagcc ggccttctgc gaattccaga ccggatcact
      841 ttcggcgtaa accggcgtgc ccgtggccag gccggcgcgg attcccgcgg cgtcgagacc
      901 gctcacgcgg gcggcattga ttgcgtacgg agaactcaga accttctgcg acggaagcat
      961 ctcctcgaag ccggctgcgg cattggtgct gaaccacacg cgcagataaa gatcctcgcc
     1021 ggcgctgaac agatcgggat ccagcgcttc catgacggct gcgtcgccaa tcgtcagact
     1081 gaacactccg ctgtacacct cgccggtcag cacaccggcc ggctcgcccg caagtgtgcc
     1141 gtcgttcgac cagaaggtgg tcgcggaatc cgcatcggcc agcgcgagtt tgaaataacc
     1201 cgtgccggtg aatgcggcgt cgcccaccag cactttgccc tgatagtcaa tttccgcgac
     1261 cggatcggcc ggcgccgaca tcgccgtgag aatcgcggca agaacaagaa ttatgcgttt
     1321 catctgcaaa ctcctaactg aaccagaaaa ttcgctctgc atgctgattt gccaatgtgc
     1381 cgtttatgcc gcgtgttgtc aatgtttgcc atccggctaa agatagagta tctatcgccc
     1441 aaccatattg gaaacaacag acatgcaggc tccagacgac accattgccg ccatcggtac
     1501 ggctcccgga accgggggaa ttgccgttgt ccgcgtttcc ggcccgcgca cgatggaaat
     1561 cgccgaccgc atcgtgcgat gcgccgcgcc gagaccctcc atgcggcgca ccatgtcggt
     1621 tatatacggg cacatcgccg ccgcggccgg cgatgtggac gaagtgctgg tccttgttat
     1681 gaaggggccc ggaagctaca cacgcgagga catgattgaa atacagggtc acggagggcg
     1741 cgtcatggcc tccgctgttc tgcagaccgt tctggatgcc ggggcgcgtc tggcggaacc
     1801 cggagaattc acaaaacgcg ctttcctgaa cggccgcatt gacctgacgc aggccgaggc
     1861 ggtcgccgac atcgttggcg cacgctcccg gcgcgccggg cgcgctgccc tggagcagct
     1921 cgaaggatcg ctgagcgatg cggtcaatgc cctttatgac cggctgatgg ggatcgccgc
     1981 cgacatggag gcgaccctgg attttccgga ggaggatatt cccgaagccc ttattcagga
     2041 tgttccggac aggatcagcc gggccgtttc ggaaatggac cttctgatat cctcctggca
     2101 ggaggggcgc ctgctccgcg aaggggccct gatcgtcata tcgggccgaa ccaatgtggg
     2161 gaagtctaca ctgttaaatg cattgcttga cactgatcgg gcaatagtat cacacattcc
     2221 cggcaccaca cgcgatatca tcgaggaagg tcttgtgctg gacggtatcc ccgtccgcct
     2281 ggtggacacc gccggcctgc gcgaaacctc gtgcgaaatc gaaagcgagg gcatccgccg
     2341 gaccatgagg cacatagcgc gggccgacct gcagctgcat gttctggatt catcgcaaga
     2401 atctggaaat gaatttgatg cgcaaatttc aggccttgac ccgatccgta ccatccttgt
     2461 cctcaacaaa actgacctcg gggttcggat ctgccgcgac aatctgaacg gcttcacctg
     2521 cgttgaaacc tcactgctga acagaaccgg tctcgaggcg ctgaagaagg aaatccggcg
     2581 ggccctgatg caggacatca aaatggatgc cccgccccac gcggtgatct ccgaacgcca
     2641 ccggcagctc ctgctgaagg cgcgcgagga tgcccttggc gcagacagcc tgttcgggca
     2701 ggatgatatc gcaatggctt cgtcccgtct gcgtgaaagc atcgaacacg tggggctcat
     2761 cacgggaaga gtatatcagg aagatctgct gaacgcggtc ttctcaaaat tctgcattgg
     2821 caagtaatta ataattaaat atatatgaat ttctcatatg acatccttgt ggccggaggg
     2881 ggtcatgccg ggtgcgaggc cgccctcgct gcagcgcgca tgggcgccag aaccgccctg
     2941 ctcacgatta accggaccac catcgcccgg atgtcctgca acccctccat tggcggcatt
     3001 gccaaatcac atctcgtgtt tgaactggat gcgctgggag gagaaattgc aaggaacacg
     3061 gatttcacgg gaatccagtt caggaagctg aacaccagaa agggacccgc cgtgcaggcg
     3121 caccgggtcc agtgcgataa aaggctttat ccgcttcgaa tgcttgcggt tatgcggaga
     3181 acagcaaacc tggacattct tgaggggatg gcagcggaag tttggattga aaccggccgg
     3241 ctgcgcggag tgacaaccga tgacggatcg aaaatcgggg cccgcgcggt gattctgact
     3301 ccgggcacat tcctgaatgg cgtgatgcat ataggaatga acagaatccc cggggggcga
     3361 accgatgaag cccccgcaaa ccggctcagc gagagcctga agagccttgg tttcctgctg
     3421 ggaaggctta aaacaggaac ccctcccagg cttcacaagg attccattga ttacagcaaa
     3481 tgccagattc aacccggaga ggtcccgcct ccgttcctgt cgcgggcggc agcccgcatt
     3541 tggaaaatgt tccacgtgga acaatcggag gtttccggca gctttttgga tgaattgttc
     3601 cacgtggaac aattcgacaa ccgtgatgcc atcaatccat cttttcaatg ctccaccgcg
     3661 cgctcatcat ccgcgcttct tccatggcct ccgggcaggg atcagcttcc ctgttatctg
     3721 acccacacca catcccaaac ccatgatatc attcgcgcca atctggagaa gagcgccctg
     3781 tatggcggcg cgatcactgg caccggggtg aggtactgtc cgtccatcga ggacaagatc
     3841 gtaaagtttc cccagcggga atcgcaccat gtatttcttg agccggaggg acgcgacaat
     3901 acccgcgtct atcccaacgg aacttccaac agcctgcctg aggatgttca gatccagatg
     3961 attcgatcca ttccggggct ggaaaaggcc gagttcattc ggcccggata cggcattgaa
     4021 tacgactatt ctgatccgac ccagctttat cattcacttg aaaccaaact ggttgagcat
     4081 ttatatttcg cgggacagat caacggcacc actggctacg aagaggcggc ggctcagggc
     4141 tttgcggccg gagtgaatgc cgtgctgcgg ctgagggggg atgcgccctt cgtccccctg
     4201 cgcaatgaag catacattgg ggtcatgctt gacgatttgg tcacaaaggg ggttgacgaa
     4261 ccgtatcgca tgttcacctc gcgcgccgag caccgtctgc tcctgcgcca aagcaatgcg
     4321 cccttccgac tcgcggacca cgccgcgcgc atcggcatcc tttctaaaac cgaagtcgca
     4381 tcggtcaggg cacaacgtga tctgattgaa ggggaggtcc ggcggctgaa tgagtcattc
     4441 ctcgataacc agagcctcgc cagcatcctc gcccgtcagg acacaaccta tgccgacctt
     4501 ctcggcgcgc tcagcggtct ggatcccctc attattaatg aaatagaaac cgacatcaaa
     4561 tacgccggct atatacggag agaacagcag aagatcgaaa agctgctcaa gctcgaacat
     4621 cagcccatcc cgcatgattt cgattacgac gccatcaggg ccctgcgttt cgagtcgcgc
     4681 gagaagctga aaaagatccg ccccgaaaac ctggggcagg cctcccgcat ctccggcgtc
     4741 aacccggccg acatctccat tctttccatc tggctgaaaa gaagccgctc acagaatgcc
     4801 tgcactgctg atggccggtg agatagttct tcaaagttcc gcaggcgtaa cggggcctta
     4861 ttcaaatgaa accgcagcga cggttcagga aacgggggcg ggggcgttca taatctcatg
     4921 gcccggtccc gcagcttctc cgaaattcta tcgggtgctg aatccataga ataagtgacc
     4981 ttttggtcat ttgtattgtg tttatacaat aatgactata tggtattgtc tattgcaata
     5041 ttatattata taataagtta tatgacgatt acggacggcg cgatggtcac ggctccacac
     5101 agaccccaag ccgatagaat ccggcgactt gcggcaggga gttggtaaag cagagttctc
     5161 cgcccgtccc gggcacgtca acgcaggttg gctcgcacgt ccagatgccc tctgcgggat
     5221 ccgctgaata atacagcgtg tacaaacggt ccgtggcact gttccagcgc ggaacaagtc
     5281 cactccccga cgcctgctcg atcagcttga agcacaggcg gctctcgaag tttgtcggca
     5341 cggttccggc aatgtattcc tgccacgccg ggaagccgtc aacatcgctg tcgctcacgg
     5401 aaagctcatc tccgttcgtg gtgacggtgt agtactgagc caaccaccag agcggcacat
     5461 cattgacggc ggtcagggcg cggaacacgg caacgaattc tccgtcggag gtcaccgtct
     5521 cccagcgatg cgtatagcag gtcaatccat ccacgccggc tagggggctt ccgttggtgc
     5581 ggatctcggc gatttcataa tagttcgatg cggtcaccgt gaagtccacg ctggccatcg
     5641 gttccacatt caccgctccg gacggcacca gccagccgcc ggcattcgcg gatgcctgta
     5701 taaccgaact ggtgggaagg gtcaccggga tgaccgacga gttcgggctg accccctccg
     5761 cgtttccggc gcggacgcgg taatagtacg tttcgcccgg cgtcaggccg gtgaccgcat
     5821 gtgaatgcac cgcgcccagc ggctggttgc taaaggtcgc gacataatcg gccggatcaa
     5881 atccggcgcc agccgacaca tcaagaaaat attcaagcgc gccggccgat gaaccccact
     5941 gtgccctgaa gctgttcacc gccaggccgg atgccgcccg ggtatccggc ggttcgggta
     6001 tggtctggag aatattcgtc cattcgttgg agtctgtgac gcgaacgcac tgaaacaaac
     6061 cgtcgctgcg atcgtcagcc gcgttgaaca gaaccgggcc cgaggctccg tcatagccgg
     6121 ccgcttccga ccggatggcg gcgcgaactt cctgaacagt tcccgtaccc ccggtatccc
     6181 gcagcgccag cgccgccagc cacagggcgt cataggcatg catggcgtac ggccggggcc
     6241 tgctgcctgt ccgcgcttcg atggcatcgc tcaccgcgga atacagcgcg ttcgtgtact
     6301 catccggctc ggtgcaggta aactgagtgt ccatggcaag ggttcgggcg gcggcattgg
     6361 tcagcagcgc agcgtttccg gccatgcctt ctgatccgta ccagcgggcg gcggccaggg
     6421 tggggaatgc cgccgcctgc tccagaatct ggacgccctc gtcaaagacc gcaagaacga
     6481 tgccgatgtt ttcggccccg acatccgccg cgcgcgcgga tacgattgca ttgagctggg
     6541 tcaccacctc cgggatgaaa cccgtggcgc gcgggcagta gccggtgtaa aacacttgcc
     6601 ctccgcgcgc ctcaaactcc tgaagcaggc gggagcgcag gccttccccg tacatatccg
     6661 agcgtgtcag cagaagcaga ttggtgatgc cgtcataaat gatgtcttcc gcgagggctt
     6721 cggcctgccg gtcatcgctg accggaaggc gcatcaggtt gtcatccgga acagctagcg
     6781 gaagcgccgt ggatgagctg gaaagcagca gcatgccgtg gtcattggcg aattccagca
     6841 ggccttcgca ttcggcactg gtctcggggc cgagcacgac ctgaacgtcc tgggtgtaca
     6901 actgctgaag ctgctccacc gccacgagcg gatcggtcac ggtgtccctg atctccagcc
     6961 ggatgggggg catgccgggg gtgttggtga cttcggccag cgcgatttca taggcggcct
     7021 gataggatgc gcccagatcg gtcagatcac cggtcagcgg gatcagcgct cctatcacgt
     7081 ttgttcctcc cggcgccagt ttgggccctg gcatcctgat cgtccggggc cccgacttct
     7141 cggctccggc cgggcccagg cctccggaac tgatgtacat ggccacgctg cagctcatgt
     7201 cgaatggata ccagttttcg tagccggcca tcgtcgtgct gttgatccag cggaacacat
     7261 cgttcagaaa atacatggtt gtgccccagc gaagagaggt cgaaaccagc gagaaatgct
     7321 ccggcatggt ggcggtcgcc aggatgcgga gcatgaccac ctcgccggtg gtggagcatc
     7381 cggacagtga aaatccccgg tagctgcaga caccgcgata ggcctgataa cttgcgcggt
     7441 cgcgcagcag gttcatccgg tacacatctc cgtaaccgtt ggacatgacc aggctgtagg
     7501 cttttgcgcc aagtctgggt gcgggcgcgg gttcgtcgag cagcgaaaag atgaaccgcg
     7561 gatcagaaac agggtcttcg ctctccgtgc gcaggggccc gacccgcttc tccggcgcct
     7621 ttgcaaggga tgcgcttccg aatcccccgg agttgatgta catggccacg ctgcagctca
     7681 tgtcgaaggg ataccagttt tcatagccgg ccatcgtcgt gctgttgatc cagcggaaca
     7741 cgtcgttcag gacatacatg gtagtgtccc agcgcttgct tgtggacagg atcgaaaaat
     7801 gctccggcat ggcggctgtg gccgggatgc ggagcataac cacctcgccg gtggtggagc
     7861 agccggacag cgagaagccc cggtagctgc acaccccgcg ataggcctga tagttgtagc
     7921 ggtcccgcag caggttcatc cggtacacat ccccgtagcc gttggtcatg acgagactgt
     7981 aggcggaaag agccgtctgt ccggcaagac agaacagagt cgccatgaac aggggccgcc
     8041 accggggttt ttctgagtgc gatttcggtt tcatggcgat atctccttgc tgaaccgcga
     8101 cgcgaatatt ttttatagca caaaaaccac ggtgatatcc atgacaaata tcatctttcc
     8161 aggcggcggg atcgctgatc atcgaagagg actttcgcat gcagaggggg gtgccggccg
     8221 gaaatagggt tggcgaagcg ggcggagccg gtataatggt gcggttcaaa tgcacacgga
     8281 tatcgagagg aggcggcatg a
//
LOCUS       DAOQWR010000119         8227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9179_length_8227_cov_4.842266, whole genome shotgun sequence.
ACCESSION   DAOQWR010000119 DAOQWR010000000
VERSION     DAOQWR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9179_length_8227_cov_4.842266"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKK36_09225"
     CDS             <1..742
                     /locus_tag="PKK36_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR94773.1"
                     /translation="DPERGSPRIAVDFGLDYREMLSNHVDYAESEASCDGDRITAGIA
                     GKTKEYFLTQLAWLKSLPGIGLAFEGWLFSPIVKEYAYEVIQRTFETVADSNARGVIG
                     YFHYPLPHRPFVWRDGRRMPTFPKDAHVHTVKNYMENMAGVDHVLGAIVKKLKDTGRY
                     DASTIVMLADHSWKQDPGDGVEGDRDEAVIQDFDADPKHRWKHVPLIIKWPHQTSGAF
                     VTNDIFTAELGNFIRDKLKENRNDTGVY"
     gene            723..887
                     /locus_tag="PKK36_09230"
     CDS             723..887
                     /locus_tag="PKK36_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94774.1"
                     /translation="MILAYIDPGSGALILQLLMAGIVGAGVYFRRAIARIFGFRKKKP
                     EDQPRADSDE"
     gene            880..2292
                     /locus_tag="PKK36_09235"
     CDS             880..2292
                     /locus_tag="PKK36_09235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94775.1"
                     /translation="MSEIQRESGSFRDPASFVFYHEGRVFRCVDEETWSCLNDMTKAG
                     LLSRMIEGGLVVATEGSAGGDIPADIQSILPPGTRCLRHRKIQQISYPYEWSFSMLAD
                     AALLHLDIQRMLIEKGLALKDASAFNVLFEGCQPVFIDLGSIERPARSDIWFAYGQFC
                     RMFLYPLLAKLHRGMDFRQVFMGSLDGLQVDQAYRMLGFWKSFAPGSFLDVFMQNLFA
                     GKKVGQATQVGQSLRVEGKTDPAVQLVNLNRLQRKIEGLKKSFRVGSMWSEYGDIRNY
                     SDQAIDEKKRVVREILNQYRPRSVLDVGCNTGEFSFMAAEAGASVMAVDYDHDAVEAL
                     YRTAKRKKMNILPLVADLSNPSPGLGLFCEERQPLLNRVGGEMVFALALIHHLMVTAC
                     MPLEAISRLFAQLTETWLVLEYIDPQDGMFRELLAIRKDYFSGFSIEKLEAGLSDHFG
                     VVSRHPLPGGLRTLLVLKKK"
     gene            2351..3577
                     /locus_tag="PKK36_09240"
     CDS             2351..3577
                     /locus_tag="PKK36_09240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94776.1"
                     /translation="MDKKSVAFALPMGLTLGGSNIWSLQMAEALRDSGHAPALLQHPP
                     IAKGRLFALEAPAGVRCIPCPWVYGSRLGRDALRSLAQSYAEVLPAVFIPNITTAAYA
                     ACAQLAVRHAEGMRIIGIAHGQGELHLQWLKYYEPVIHRFIAVSDEVAELLQESIPHR
                     TGDIFTKACAVDIPPDSNRSYSAAGQPLQIVYAGRITNHEKRVHLLIPLAEALVKRGV
                     DFRLRIIGDGGFRWGLNKAVRELPPEVQKHIAVEGPCPPKEMAAVWAVADVCVLVSDR
                     EGTAVSMLEAMAHGCVPVVTRVSGTAAVVEHGVNGFVSDIDDLEAMTDHIVKLADDRS
                     LLATCGRRGHEKIAAGYALADYRSWFLDLIQAAWDDKPRAWDASRPVVMSGRPSPRPG
                     VWSALGRVFARREAPA"
     gene            3581..5035
                     /locus_tag="PKK36_09245"
     CDS             3581..5035
                     /locus_tag="PKK36_09245"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNR94777.1"
                     /translation="MSFNSFSYALFLPLVLLVTHVLRRRVAGRNAFLLTASYFFYGSW
                     DWRFLSLIWISTGVDYICGRLLDWKDMDVPIPDEAWPRRKAILLISLLCNLVLLGFFK
                     YYGFFVESAAAFLETIGFRAHLPALNIVLPVGISFYTFQTLSYTIDVYYGKLRAERNL
                     LNFALFVAFFPQLVAGPIERARDLLPQMRMARPVALEVFYSGFYLIGWGLFKKVVIAD
                     NAAILANDVFAASSPAGLASLIGVYAFALQIYCDFSGYSDIARGTARCMGFDLMLNFN
                     LPYFAVNPSDFWRRWHISLSTWLRDYLYIPLGGNRRGIRRTYINLMLTMVLGGLWHGA
                     GWTFVCWGIYHGLLLCFHRVFQPGMARLAAPVEKSFPVSWFAARVFLFFHLTCISWLL
                     FRAESIGQAWSMFRAVMTDWTLGGQVGLSAMMQVGVCAAGLVCIQIAQHVKKDMDVVL
                     HSPLPVRALLYLAGMLGFIFLGEYGGQSFIYFQF"
     gene            5001..6089
                     /locus_tag="PKK36_09250"
     CDS             5001..6089
                     /locus_tag="PKK36_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94778.1"
                     /translation="MADSHSSISSSDPKCLPRAFLLALLLFLMLETGLRFVDPRHLIP
                     YNENFPFEHYAVRYYLETAGPADISFIGSSRIREAVHLPTLADLLAGEEDLSRMSCAN
                     YGCSGASADVVEAVARLMMRQEKKPALIIYGVSPKQYLGRESNLDVCSVYWNFGDWYR
                     VRSLHGEKAAKYFPDVFRTAIGRASLVFRYRKRPGDLASRLLSKKQVLTCPIRGEPSA
                     WHTKLAGRNMRTTPTTPDKVREHLEYYLVDGEYPMSEEKLKCVQNVIDLCQSNGIPVV
                     LLEVPVADILLEHYPPGTQEKFLEMTQRLSVQNNVPFFRSAELGAVLSDESFSEESHL
                     GVKGAVVYTKAVYQTVIRPRLKPVASGP"
     gene            6108..7316
                     /locus_tag="PKK36_09255"
     CDS             6108..7316
                     /locus_tag="PKK36_09255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR94779.1"
                     /translation="MPEKPVIVFVWPNMHVYGGVHTFLLDCLQRLPGMDFRPYVFDVG
                     RGQGPLDEYLKGFGHAIQRFIPSRGEDDVHYWNRVHAGLRDLSPSLVVFIESRYAEDV
                     LKGLPEDVAAINLCLVDRPDEKYYRHARALAPRMKLIAGNSARIVARLRDELPADLHS
                     RIRYLPIGVEAPPDAGVRSLHDPIRLIFMARLQYKQKRAQDLVPFARELAALRVDFHL
                     SIVGQGEAADFLQHELSELISSGTVEMLPVVGPPARQLLNDYDVSLLFSEYEGQPMTV
                     LDALVRGIVPVVSDVKSGMADILKHGENGLLFPIGRPDIAARLVQALADDREYFASLS
                     RGALELGRHFIVDETMLKLAQIIRESLSEMPAINPWDGWLPAPGTSRGRLIGYARPLL
                     ARLRKALRGD"
     gene            7334..8068
                     /locus_tag="PKK36_09260"
     CDS             7334..8068
                     /locus_tag="PKK36_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR94780.1"
                     /translation="MDTTDLFTSEYLTAIQKGTERCASVVASRLTGLMNIGSVIDIGC
                     GNGAWLAEFMRAGVQDVLGVDGPYVPGSILQIPADKYVGMDLRRRMSLNRTCDLVLCL
                     EVAHYLDANDHPAFADYLAALSPCILFSCAIPGQPGKNHVSPQWHDYWHRLFQQRDYA
                     AFDFIRPELWHNENIPLHFRQNIFLYVRSDHLQDQAMKNIAPLARANCLTLVDMDSIR
                     TEMALRATLRRLPILLRKKLFGRGGV"
     gene            8074..>8227
                     /locus_tag="PKK36_09265"
     CDS             8074..>8227
                     /locus_tag="PKK36_09265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNR94781.1"
                     /translation="MEQPLVTAILCTYNRESYLREALDSILAQTYPNIEVLVIDDGSS
                     NAGMFRI"
BASE COUNT         1591 a         2108 c         2530 g         1998 t
ORIGIN      
        1 tgatcccgag cggggcagcc ccagaatcgc agttgatttc ggcctggatt accgcgaaat
       61 gctttcgaat catgtggatt atgcggaatc agaggccagt tgtgacggag atcggattac
      121 ggcgggcatt gccgggaaaa ccaaagagta ttttctcacg cagctggcat ggctgaagtc
      181 tctgccgggc attgggctcg catttgaggg atggttgttc tctccaatcg tcaaggagta
      241 cgcctacgag gtgatacagc gcactttcga aacggtggcg gattcgaatg cccggggcgt
      301 cattggttat tttcactatc ctctgccgca ccggccgttt gtatggcgcg acggtcgccg
      361 catgccgacg ttcccaaaag atgcccatgt gcataccgta aaaaactata tggaaaacat
      421 ggctggcgtt gaccatgtgc tgggcgcgat cgttaaaaag ctgaaggata cgggaagata
      481 tgacgcttcc accatagtga tgctggccga ccacagctgg aaacaggatc ccggtgacgg
      541 ggttgagggg gaccgggacg aagcagtcat tcaggacttt gatgcggatc cgaagcatcg
      601 ctggaaacat gtgccgctga tcatcaagtg gccgcatcag acgtctggcg cttttgtgac
      661 gaatgatatc tttaccgcgg agcttggaaa ttttataaga gacaaactga aggagaacag
      721 aaatgatact ggcgtatatt gatccgggtt cgggtgcgtt gattctgcag ctgctgatgg
      781 caggtattgt gggtgcgggc gtctatttcc gccgcgccat cgcccgcatt ttcggcttcc
      841 gcaaaaagaa accggaagac cagcccaggg ctgactctga tgagtgaaat acagcgcgag
      901 agcggttcct tcagggatcc ggccagcttt gtcttctatc atgagggccg ggtcttccgg
      961 tgcgtggatg aggaaacctg gtcctgtctg aatgacatga caaaggccgg tctgctttcc
     1021 cggatgattg aagggggcct ggttgttgca acagagggtt cagcaggagg ggatattccc
     1081 gctgatattc agagcattct tccgccgggc acacgctgtc tccggcaccg gaaaatccaa
     1141 caaatcagtt atccgtacga gtggtctttc tccatgctgg cggatgccgc gctgctgcat
     1201 ctggatattc agcgaatgct gatcgaaaag ggcctcgcgc tgaaggatgc cagcgccttc
     1261 aatgtgcttt ttgagggctg ccagcccgtg ttcattgacc tgggctctat cgaaaggccc
     1321 gcgcgcagcg acatctggtt tgcttacggc cagttttgca ggatgttcct ttatcccctg
     1381 ctggcaaagc ttcaccgggg catggatttc cgccaggtgt ttatgggcag ccttgacggc
     1441 cttcaggtcg atcaggcata tcgcatgctg ggtttctgga agagctttgc gcccggttct
     1501 tttctggatg tgttcatgca gaacctgttc gccgggaaga aggtcggtca ggccacgcag
     1561 gttggccagt cgctcagggt ggaaggtaaa actgaccccg cagtccaact ggtcaatttg
     1621 aaccgcctgc agcggaagat agaaggtttg aagaaatcct tccgggtggg aagcatgtgg
     1681 agcgaatacg gggacatccg caactattct gatcaggcga tagatgaaaa gaagcgcgtg
     1741 gtgcgggaga tattgaacca gtatcggccc cggtcggtgc tggatgtcgg atgcaatacg
     1801 ggcgagttca gtttcatggc ggccgaggct ggcgcatctg ttatggctgt ggattatgat
     1861 cacgatgccg tggaggcctt gtacagaact gcaaaacgga agaagatgaa cattctgccc
     1921 ctcgttgccg atctttccaa tccaagtccg gggttgggtc tgttctgtga ggagcgccag
     1981 cctcttctga atcgggttgg cggagaaatg gtgttcgcgc tggccttgat ccatcacctg
     2041 atggtgacgg cctgcatgcc gctggaggct atcagccgtc tgttcgcaca actgacggag
     2101 acctggctgg tccttgagta cattgatccg caggatggca tgttccgtga actgctcgcc
     2161 attcggaaag actacttcag cgggttcagc atcgagaaac tggaggcggg cctgtccgat
     2221 cacttcgggg tggtgtcccg tcacccgctg ccgggcggcc taagaaccct tctggtgttg
     2281 aaaaagaaat aatagcggcc tgaaaacgtc gtcagtccgc atgacccggc gccggccatc
     2341 cggtacccat atggataaga aatccgttgc ctttgcgctg ccgatggggc ttacactggg
     2401 cggctccaat atctggtcgc tgcaaatggc cgaagcattg cgagattcgg ggcatgctcc
     2461 ggcgctcctc cagcacccgc ccatcgcgaa gggcaggttg tttgcgctgg aagcgccggc
     2521 gggtgttcgg tgcattcctt gtccctgggt ttatggatcc cgtctggggc gggacgcttt
     2581 gcgcagtctg gcgcagtcct atgcggaggt attgcctgcg gtgttcatcc cgaatattac
     2641 aacggcggcc tatgccgcct gcgcacaact ggccgtgcgg catgcggaag gtatgcgcat
     2701 tatcggaata gcccatggcc agggggaact tcatctgcag tggctcaagt attatgaacc
     2761 ggtcattcac cggtttatcg cggtcagcga tgaggtggca gaactccttc aggagagcat
     2821 cccgcaccgc acgggagata ttttcacaaa agcctgtgcg gtggacattc ccccggattc
     2881 caatcggagt tattcggcgg ccggccagcc tttgcagatc gtatatgctg gaaggattac
     2941 aaatcatgaa aagcgggtgc atctgcttat cccgcttgcc gaggcgctgg tgaagcgggg
     3001 cgttgatttc cgcttgcgca tcattgggga tggcggtttc cgatgggggt tgaacaaagc
     3061 cgttcgggaa cttcctcccg aagtgcagaa acacatcgct gttgaaggcc cttgtcctcc
     3121 aaaggagatg gctgccgtgt gggccgtggc agatgtctgt gtgctggtgt cagacaggga
     3181 aggtacggcc gtatccatgc ttgaagccat ggcgcacggc tgtgtcccgg tcgtgacgcg
     3241 cgtgagcggc acggcggcgg ttgttgaaca cggagtcaac ggcttcgtgt cggacattga
     3301 tgatctcgag gcgatgaccg atcacattgt gaaactcgca gatgaccggt cgctgctggc
     3361 gacatgcggc cggaggggtc atgagaaaat cgctgccggg tatgcgctgg ctgattaccg
     3421 gagctggttt ctggatttga ttcaggcggc atgggatgac aagccgcgcg catgggatgc
     3481 atcacggccg gttgttatgt ccgggcgtcc ttccccccgt ccgggggttt ggtctgcgct
     3541 tggacgagtg ttcgcccggc gggaggctcc ggcatagaac atgagtttca acagtttcag
     3601 ttacgcgctg tttctgccac tggtgctgct ggtgacgcat gttctgcggc gtcgcgttgc
     3661 ggggcggaat gcattcctgc tgacggccag ctatttcttc tacggctcat gggactggcg
     3721 tttcctgagc ctgatctgga tttcaacggg cgttgattat atttgcggca ggcttctgga
     3781 ctggaaggat atggatgttc cgatcccgga cgaggcctgg cctcgccgga aagccatttt
     3841 gttaatcagc cttttgtgta acctggtatt actggggttt ttcaagtact acggtttctt
     3901 tgttgagagc gcggctgctt ttctggaaac tatcggattc cgggctcatt tgcccgcgct
     3961 caatatagtt cttcccgtcg gtatcagctt ttatacattc cagacgctta gttataccat
     4021 tgacgtgtac tacgggaaac tgcgcgcgga acgaaacctc ctgaattttg cgctttttgt
     4081 ggcattcttc ccgcagcttg tcgcggggcc catagaacgc gcccgcgacc tgctgccgca
     4141 aatgcgcatg gcgcggcccg ttgcgctgga agtgttctac tctggatttt atctgatcgg
     4201 ctggggtctg ttcaaaaaag tagtgattgc ggacaatgcg gcgattctgg ctaacgatgt
     4261 tttcgcagca tcatcgccgg cgggtctggc ctcgctgatt ggggtctatg ctttcgccct
     4321 tcagatatac tgcgactttt cgggatattc cgatatcgcc cgcggcacag cccggtgcat
     4381 gggctttgat ctgatgctga atttcaatct gccgtatttc gcggtgaatc ccagcgactt
     4441 ctggcggcga tggcatatca gtctcagcac ctggcttcgg gattacctgt atatcccgct
     4501 gggcgggaac cggcgcggga tcagaaggac gtatatcaac ttaatgctta cgatggtgct
     4561 gggcggcctg tggcacggag caggatggac atttgtctgc tggggaattt atcacgggct
     4621 cctgttgtgt tttcaccggg tgttccagcc gggcatggca cggctggcgg ccccggtgga
     4681 gaaaagtttc ccggtttcat ggtttgccgc aagggtattc ctgttcttcc atctgacctg
     4741 catcagctgg ctgctgttcc gcgccgaatc cataggacag gcctggagca tgttcagggc
     4801 tgttatgacg gactggacgc tgggcggaca ggttggtttg tcggcaatga tgcaggtcgg
     4861 ggtgtgcgcg gcgggactgg tatgcattca gattgctcag catgtgaaaa aggatatgga
     4921 tgttgtgctt cattctccgc tgccggtaag ggcgttgttg tatctggcgg gcatgctggg
     4981 tttcattttc ctgggtgaat atggcggaca gtcattcatc tatttccagt tctgatccaa
     5041 aatgcctccc gcgggcattt ctgctggcgc tgctcctttt tcttatgctg gagacggggc
     5101 tgcgctttgt ggatccccgt catctgattc cgtacaacga gaacttcccg tttgagcact
     5161 atgcggtgcg atactatctg gagactgcag ggcctgcgga catttctttt attgggtcga
     5221 gccggatacg cgaggcggtg catctcccga cgcttgcaga tttgctggcc ggcgaagagg
     5281 atctttcgcg gatgtcctgc gccaattacg gctgctccgg cgccagcgct gatgtggtgg
     5341 aggccgttgc gcgcttgatg atgcggcagg agaagaagcc cgccctgatc atttacgggg
     5401 taagccccaa gcagtatctg ggccgcgaaa gcaatctgga tgtgtgttcc gtctattgga
     5461 atttcggaga ctggtacagg gtccgcagtc tgcatggaga gaaggcagcg aagtatttcc
     5521 cggatgtttt ccggaccgcg atcggaaggg ccagcctcgt gttccgctat cgcaagaggc
     5581 cgggcgatct tgcaagccgc ttgctttcaa aaaaacaggt gctgacctgt ccaatacgcg
     5641 gggaaccttc tgcctggcac accaaactgg ctggacggaa tatgagaaca actcccacta
     5701 cacccgacaa ggttcgcgag cacctggagt attaccttgt cgacggcgaa tatcccatgt
     5761 ccgaagagaa gctcaaatgc gtgcagaatg ttattgatct ctgccaatcg aacgggattc
     5821 ctgtcgtatt gctggaggtt cccgttgcgg acattctgct ggagcattat cctccaggaa
     5881 cgcaggaaaa attcctcgaa atgactcagc gtctttccgt acagaataat gttcccttct
     5941 tccgcagcgc tgagcttggc gctgttttgt ctgacgagtc tttctctgag gagtcgcatc
     6001 ttggcgtgaa gggcgcggta gtttacacga aagcggtata tcagacggtg attcgccccc
     6061 ggttgaagcc ggtcgcatcg ggcccgtgag cttaacgcac tgcaatcatg cctgaaaagc
     6121 ctgtcattgt ttttgtttgg cccaatatgc atgtctatgg gggcgtgcac acttttcttc
     6181 tggactgcct ccagcgcctg cccggcatgg actttcggcc gtatgtgttt gatgtggggc
     6241 gcgggcaggg ccccctggat gaatatctga aaggatttgg gcatgccatc cagcgcttta
     6301 ttccttcgcg tggtgaagac gatgtccatt actggaacag ggtgcatgcc ggtctgcgtg
     6361 atttgtctcc gtcactggtt gtgttcattg aatcgcgcta tgccgaggat gtcctgaagg
     6421 ggcttccgga ggatgtcgcc gcaatcaatc tttgtctagt ggaccggcct gatgagaaat
     6481 actaccgaca tgccagggcg ctggccccgc gcatgaagct gattgcgggc aacagcgcgc
     6541 gcatcgtcgc ccgcctgcgg gatgaactgc ccgcggacct ccattcccgg atacgctact
     6601 tgccgattgg cgtggaggcg ccccctgatg cgggggtgcg gtccctgcat gatccgatcc
     6661 ggctgatttt catggccaga ctgcagtata aacagaagcg ggcacaggat ctggtccctt
     6721 ttgcgcgtga acttgctgca ttgagagttg atttccatct cagcatagtg gggcagggag
     6781 aggccgcgga ttttctgcag catgaactgt ctgaactgat cagttccggc accgtggaaa
     6841 tgctgcccgt tgtgggcccg cctgcccgcc agctgcttaa tgattatgac gtctctcttc
     6901 tgttttctga atatgagggg cagcccatga ctgtgctgga tgcactggtg aggggcatcg
     6961 tccccgtggt gtcggatgtc aagagcggga tggcggacat tctgaagcat ggcgaaaatg
     7021 gattgctttt cccgataggc cggccggata ttgctgcgcg cctggtccag gccctggcag
     7081 atgaccggga atactttgca agcctgagcc gaggagcgct ggaactgggc aggcatttca
     7141 tcgttgatga gaccatgttg aaactggcac agattatccg agaatcgctt tcggaaatgc
     7201 ccgcgatcaa cccctgggat ggatggctcc cggcccccgg cacatcccgc ggccggttga
     7261 tcggatatgc aagacccctg ctggcccggc tcaggaaggc gcttcgcggg gattgatcgc
     7321 ggggatatct gatatggaca cgacagacct tttcacctcg gaatatttaa cggccattca
     7381 gaagggcacg gagcgctgtg cctccgttgt ggcaagccgg ctgacgggcc ttatgaatat
     7441 tggcagcgtg attgatatcg gctgcggcaa cggggcgtgg ctggcggaat tcatgcgggc
     7501 gggagtgcag gacgttctcg gggtggatgg tccgtatgtc ccgggaagca ttcttcagat
     7561 tcccgctgac aagtatgtag ggatggattt gcgccgccga atgtccctga accggacttg
     7621 cgatctggtc ctttgtctgg aggtggctca ttatctggat gcgaacgacc atccggcctt
     7681 tgcggattat ctggccgccc tgtcaccctg catccttttc tcctgcgcca tcccgggcca
     7741 gccgggcaaa aatcatgtga gtccgcagtg gcatgactac tggcaccggc tgtttcaaca
     7801 gcgggactat gcggcctttg atttcatccg gccggaactc tggcacaacg aaaacattcc
     7861 gctgcatttt cgccagaata tttttctcta tgttcgcagc gatcatcttc aggatcaggc
     7921 catgaagaac attgcgccgc tggcgcgcgc caactgcctg acgctggtgg atatggattc
     7981 cattcgaacc gagatggcgc tgcgcgcaac attgaggcgt ttgccgattc tgctgcggaa
     8041 gaagttgttc ggacggggag gggtttgacg gagatggaac agcctctggt caccgcgatt
     8101 ctttgcacct acaaccggga atcgtatctc cgggaggcgc tggacagcat tctggcccag
     8161 acctatccca atatcgaggt gctggtgatt gatgacggtt cctccaatgc ggggatgttc
     8221 cggattc
//
LOCUS       DAOQWR010000120         8157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9279_length_8157_cov_4.765490, whole genome shotgun sequence.
ACCESSION   DAOQWR010000120 DAOQWR010000000
VERSION     DAOQWR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9279_length_8157_cov_4.765490"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKK36_09270"
     CDS             <1..513
                     /locus_tag="PKK36_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNR94782.1"
                     /translation="EHDHGDQARKVEEGAAEVVRSSSSGDAQTVAELKPGAAFGEEAL
                     ISFAPRNATVTMLSDGVVLRLHKQHFAEHIQNAVIRWLSPAEAKKEVEAGARWVDVRD
                     IHEFDRSHLADALHIPMQDLRERMGGLDKERLYVCYCSNGRRSATATFILRQQGFNAA
                     VLRGGLKSFS"
     gene            655..2412
                     /locus_tag="PKK36_09275"
     CDS             655..2412
                     /locus_tag="PKK36_09275"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNR94783.1"
                     /translation="MSIQEWMLFAFRVIGGMSLFIFGMHVMTQGLRAAAGEALRVILA
                     RAARRRWEGLLLGALVGFLAHSGAATTMVAGFVNAGLMTLAESLPPMLGANVGTALSM
                     QLISFHIGHYCWMAIGIGYIMSAVAPGRRVRESGHALLGFGLLFLGMTTISEAMAPQK
                     ELLAPLLKHIHGETWSGRLAGVGLSTLLTALVTSSGAVIGLCFALISAGVCTSFEQIF
                     PIVVGAHIGTCIVAMTASAAMNVEARRSALGHLVFNIFNVALALAAYPVIARVMQLGG
                     FSLSRQAANLHLLVMAVSALLVLPVTPLMARLLRVLWPSREPPAEPSYLKDELIERPE
                     QALCATVRELRRAARICVQCMDNNAGVMLKGSRRNLRRLYADEAVINEVKIAMNYFLS
                     RLTARRLTRRQTLFMQHLHRCMKDIERIGDHLTALADISIARLKIPAAIVDESIFRIW
                     YDLFLAARQVLIAVEQSLDADAGDFQVRALDILNARDCYAIMSMDAKAEFAGAVADKK
                     ITASGGYFFNRYITDLDRLVRHAKNIAFSERQQDFRIKPEHFDETASPVSLQAPANLA
                     RAESYLAELQKPGDFLKRG"
     gene            2518..3198
                     /locus_tag="PKK36_09280"
     CDS             2518..3198
                     /locus_tag="PKK36_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNR94784.1"
                     /translation="MSVQDNISRIHERMAAACMRAGRDPGSVRLVAVSKKHQPESIME
                     AVAAGQTVFGESRVQEAVAKIPRCPGGLSWHMIGHLQSNKVRQVVQYFDMIHSIDSLR
                     LLEAVNQAAEQAGRTLKVMLEVNVSGESVKFGLKPEDAPSVLEASNRLMNIELTGLMT
                     MPPMTALAEGARPHFRRLRELRDAWRAATGIPLEELSMGMSHDFEVAIEEGATWIRVG
                     TDIFGERA"
     gene            3195..4025
                     /gene="proC"
                     /locus_tag="PKK36_09285"
     CDS             3195..4025
                     /gene="proC"
                     /locus_tag="PKK36_09285"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNR94785.1"
                     /translation="MMKNLSSLKTVFIGAGNMAEALVAGMIREGLCNPAQITVTDVLQ
                     ERLDLFASKFGVAVSRDNAAAEGADIAVLAVKPQQMPAVLEALRPSAEKIGQTLFISI
                     AAGIPTSGIEAGLGKGSRVVRAMPNTPALAGKGAAAICTGKYADEDDLELADILLGAV
                     GLVVRAQEDDMDAVTAVSGSGPAYVFYLIEAMQQAAKNMGLDPEVSRDLVLATVEGAA
                     VLCRETGLGADELRLRVTSKGGTTAAAMDVLEERKVFAALLDAMTAARNRAHELSEQN
                     "
     gene            4036..4953
                     /locus_tag="PKK36_09290"
     CDS             4036..4953
                     /locus_tag="PKK36_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94786.1"
                     /translation="MASRTKRKKAGADQPVEGHPHVLAHFILGCVGLLAVAYGAALLL
                     SRTDGMRYLVGDRLEQQLGLPVRIGAMRLTPGLDLVLENVTTDDARTIQVERLEISGS
                     LLQKLKGRPWLQSAEITGCRLAMTQSPGGRWSPAACMPMSEWLVRWCGIRIPSLPLTG
                     APGQAKDSAPGEKTLDAADLGVNESRGFHHQIPLKLRNGKIIWQDAQGGELASAGDIE
                     VHITPLRTQTREMQHAVFSAGHVRSAAGDYITGIFVELLSTQGHHVLLAQRANRTRSD
                     TPPSGAYQQAYEYGVPDYLPDDPRGEPAP"
     gene            5006..5081
                     /locus_tag="PKK36_09295"
     tRNA            5006..5081
                     /locus_tag="PKK36_09295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5039..5041,aa:Thr,seq:cgt)
     gene            5105..5824
                     /locus_tag="PKK36_09300"
     CDS             5105..5824
                     /locus_tag="PKK36_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNR94787.1"
                     /translation="MPEKTFNVDDVAEYLHIAAREIERLAREGEIPCARRGSRLEFRR
                     REVDAWASRRIMNFSGGDLTSYHRVSSARNHDLNKSHAIIPEMMRVDYVDAAMTSKTQ
                     PSVIRDMVALAGRTGLLVYPEDLLESLVKREGLGSTAMTGGFAILHPQHHEPYMFEDS
                     FIALGRTVQPVPFSSPDGQRTSIFFLLCCQDDRTHLHLLARICMMCLQTSLIMDLQLA
                     ADAQEMMNCMIATEEQIIRGL"
     gene            complement(5874..6632)
                     /gene="surE"
                     /locus_tag="PKK36_09305"
     CDS             complement(5874..6632)
                     /gene="surE"
                     /locus_tag="PKK36_09305"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNR94788.1"
                     /translation="MNILLTNDDGIYAPGIEALHRALKDMGRVSVVAPDSQRSAVGHA
                     ITLSDPIKSVAIQRDGRLFGHATSGTPADCVKLAVCALLDHKPDLVISGINLGPNAGI
                     AAIYSGTVSAATEGAILGLQSVAISIDTFTDPIWETPARVMQKLIPVLQKHPLPRDTV
                     LNVNVPNLPYDQLKGFAATRMARSRYVEKFDKRADPRGNIYFWMDGDMEFTGERDHTD
                     LDMLAKGYVSMTPIGFDLTRHELVDTVAGWKVSL"
     gene            6747..7011
                     /gene="ffs"
                     /locus_tag="PKK36_09310"
     ncRNA           6747..7011
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK36_09310"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            7038..>8157
                     /gene="dnaX"
                     /locus_tag="PKK36_09315"
     CDS             7038..>8157
                     /gene="dnaX"
                     /locus_tag="PKK36_09315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNR94789.1"
                     /translation="MAYEVLARKWRPQQFDDVVGQRHVTDTLKNAIKTGRIAHAYLFV
                     GPRGIGKTTSARIFAKALNCEKGPTATPCDKCDSCREIMSGRSLDVIEIDGASNRGIE
                     HIRNLRENVCYAPARGPYKIYIIDEVHMLTSESFNALLKTLEEPPPHVKFVFATTEPH
                     KILPTILSRCQRFDLLPIPNQEIITHLGMIAKAEKIDIDEDALLAIARGAEGGLRDAE
                     SALDQLVSFCGAKIREPDVMSVFGLVSRQMLERLVDAMLAGDISAVVKQVAELADSGK
                     DMLRVVHELLEYFRNILVYHASKGSEEMLGLGAAQMEAVKRQSESAGPERILQIIEIL
                     TDTASRLGRALSRRTLLEIALIRCSRAATAVSIDAILSQ"
BASE COUNT         1571 a         2382 c         2580 g         1624 t
ORIGIN      
        1 gaacacgatc acggcgacca ggctcggaaa gtagaggagg gcgcggccga ggtggtgcga
       61 agcagctctt ccggcgacgc gcaaaccgtg gcagaactga aacccggcgc tgcattcggc
      121 gaggaggccc taatttcgtt cgcaccgcgc aatgccaccg tcaccatgct ttctgatgga
      181 gtggtcctgc ggcttcacaa gcagcatttc gccgagcaca tccagaatgc ggtcattcgc
      241 tggctttctc cggccgaggc aaagaaggag gtggaggccg gggcccgctg ggtggatgtc
      301 cgcgacattc atgaattcga ccggtcccat ctggccgatg ccctgcatat tccgatgcag
      361 gacctgcgcg aaagaatggg ggggctcgac aaggaaagac tctatgtctg ctactgctcc
      421 aacggccgga gaagcgccac cgccaccttt atcctgcggc agcagggctt caacgccgcc
      481 gtcctgcgcg gggggctgaa aagcttttcc tgacgtctcc gtccaccctg tcctgccgcc
      541 attaaaatat tccaaaattg attttctttt tcgcggggaa agagcataac tgcgtcttgt
      601 cacaggccgt aacggccgca tgcctccagg gctttggagg ccggagcgat taccatgagt
      661 atacaggaat ggatgttgtt tgcatttcgg gtgattggcg gcatgagcct ctttatcttt
      721 ggaatgcacg tgatgacgca gggtctgcgg gccgccgcgg gtgaggcgct ccgcgtgatc
      781 ctggcccgcg ccgcccgcag gcgctgggag ggccttctgc tcggcgccct ggtgggcttt
      841 ctcgcgcaca gcggggccgc caccaccatg gtcgccggat ttgtgaatgc cgggctgatg
      901 acactcgcgg aatccctgcc gccgatgctg ggcgcgaatg tcggcacagc gctttcgatg
      961 cagctgattt cctttcacat cggccactac tgctggatgg ccatcggaat cggttacatc
     1021 atgagcgccg tggcccccgg ccggcgtgtg cgcgaatccg gtcacgccct cctcgggttt
     1081 ggactgctgt ttctcggcat gaccaccatc agcgaggcca tggccccgca gaaagaactt
     1141 ctggccccgc ttctgaagca cattcatggt gaaacgtggt cgggccgtct cgccggtgtg
     1201 ggcctgtcta ccctgctgac cgcgctggtc accagcagcg gcgcggtcat cggattgtgc
     1261 tttgcgctga tttcggccgg agtgtgcaca tcctttgaac agatattccc gattgttgtg
     1321 ggagcgcaca tcggcacctg catcgtggcc atgacggcca gcgccgccat gaacgtggag
     1381 gcgcgccgct cggcgctggg gcatctggtg ttcaacatct tcaatgtggc tctcgcgctg
     1441 gcggcttatc cggtcatcgc gagggtcatg cagcttggcg gcttcagtct ttcgcggcag
     1501 gcggccaacc tgcatctgct ggtcatggca gtgtccgcac tgctggtact tccggtgaca
     1561 ccgctgatgg cccgcctgct gcgtgtgctc tggccttcgc gcgagccccc ggccgaaccc
     1621 agctatctga aagatgagct cattgaacgg cccgagcagg cgctttgcgc cacggttcgc
     1681 gaactccgcc gcgcggcgcg gatctgcgtg cagtgcatgg ataacaacgc cggcgtcatg
     1741 ctgaagggtt cccgcagaaa cctgcgccgt ctctatgctg acgaagccgt catcaacgaa
     1801 gttaaaatcg caatgaacta tttcctcagc cgcctgacgg cccgtcggct cacgcggagg
     1861 cagacactct tcatgcagca tcttcaccgc tgcatgaagg atattgagcg gatcggggat
     1921 cacctgaccg cgctggcgga tatttccata gcgcgcctga aaataccggc cgccattgtg
     1981 gacgaatcca tcttccgcat ctggtatgac ctgtttcttg ccgcccggca ggtcctcatc
     2041 gctgtcgaac agtcactgga cgcggatgca ggcgacttcc aggtccgcgc gctggatatt
     2101 ctgaacgcgc gcgactgcta tgccatcatg agcatggatg ccaaggccga gttcgccggg
     2161 gccgtggccg acaagaaaat cacggcgtcc ggcggctact tcttcaaccg ctacatcact
     2221 gatcttgacc ggctcgtgcg ccacgcaaaa aacatcgcat tctccgagcg gcagcaggac
     2281 ttccggatca agccggagca ttttgacgag acggcttctc ctgtaagtct tcaggcaccc
     2341 gcaaatctgg cccgcgcgga gagctatctc gctgaacttc agaagcccgg tgatttcctg
     2401 aagaggggtt gatgcggttc cgcccgcgaa aacttccaat gcctggaact tttcgtgaaa
     2461 aaacttccaa tgattggaac tttttgagac aaaacttcca atgattggaa ctttttcatg
     2521 agcgtgcagg ataatatctc tcggattcat gaacgcatgg ccgccgcctg catgcgggcc
     2581 ggccgcgatc ccggctctgt ccggctggtt gccgtgtcca aaaaacatca gccggaaagc
     2641 atcatggaag ccgtggcggc gggtcagacg gttttcggcg aaagccgcgt ccaggaggcc
     2701 gtcgcaaaaa tcccccgctg tccgggcggg ctgtcgtggc atatgatagg ccatctgcag
     2761 agcaacaagg tccggcaggt cgtgcagtat tttgacatga ttcactcgat tgattcgctc
     2821 cgcctgctgg aggctgtgaa ccaggccgcg gaacaggccg gccggacgct gaaggtgatg
     2881 ctggaggtga atgtttccgg agaaagcgtg aagttcggcc tgaagcctga ggatgccccg
     2941 tccgttcttg aggcttccaa ccgcctgatg aatatagagc tcaccggcct catgaccatg
     3001 ccgcccatga cggcgctggc cgagggcgcg cggccccatt tccgccggct gcgcgaactg
     3061 cgcgatgcat ggcgcgcggc gaccggcatc cccctggaag agctgtccat gggcatgtcg
     3121 cacgattttg aagtcgccat tgaagagggc gccacctgga tcagggtggg cacggatatt
     3181 ttcggagaac gcgcatgatg aaaaatttat cctcgctgaa gacggttttc attggcgccg
     3241 gcaacatggc cgaggcgctc gtggcgggca tgatccgcga ggggttgtgc aaccccgcac
     3301 agatcaccgt caccgatgtg ctacaggagc ggctggacct gtttgcatca aagttcggag
     3361 tcgcggtttc gcgggacaat gccgcggccg aaggggctga tattgcggtg ctggcagtga
     3421 agccgcagca gatgcccgcc gtgctcgaag ccctgcgtcc ttctgctgag aaaatcggac
     3481 agaccctctt cataagtatt gcggcgggta taccgacttc cggcattgaa gcgggtcttg
     3541 gcaaagggtc gcgtgtggtg cgcgccatgc ccaacacgcc cgcgctggct ggaaaaggcg
     3601 cggcggcgat ctgcaccgga aaatatgcag atgaagatga tctggaactg gcggatatcc
     3661 tgctgggggc cgtcggcctg gtggtccgcg cgcaggagga cgatatggat gcggtcaccg
     3721 ccgtcagcgg gagcggtccc gcctatgtgt tttatctgat cgaggccatg cagcaggccg
     3781 caaagaatat gggcctcgat ccggaggtgt cgcgcgacct ggtgctggcc acggtggagg
     3841 gcgctgcggt gctttgccgc gagacgggcc tgggggccga tgaactgcgt cttcgtgtca
     3901 cctcgaaggg gggcacgacg gccgccgcga tggacgtgct ggaggagcgc aaggtcttcg
     3961 cggccctgct ggatgccatg acggcggcgc gcaatcgggc gcacgaactg tcggaacaga
     4021 actgaaccgc ccgccatggc cagccgcaca aaaagaaaga aagccggcgc cgatcagcct
     4081 gtcgaaggtc atccccatgt ccttgcgcat ttcatcctgg ggtgcgttgg cctgctggca
     4141 gttgcgtatg gcgcggccct tctgctttcc cggacggacg gcatgcgata tctggtcggc
     4201 gacaggcttg aacagcagct cggcttgccg gtgcggatcg gggccatgcg gctgacgccc
     4261 ggcctggatc tggttctcga gaatgtcacg acggacgatg cccggacgat tcaggtggag
     4321 cggctggaaa tcagcggcag tttgctgcag aaactgaaag gccggccatg gcttcagtcc
     4381 gcagaaatca ccggttgcag gctggcgatg acgcagtcgc ccggcggccg gtggagtccg
     4441 gccgcctgca tgccgatgag cgaatggctg gtgcgctggt gcggaatccg tattccgtct
     4501 ttacctctga cgggtgcgcc gggacaggcg aaagattcag ctcccggaga gaagacactg
     4561 gatgccgccg atctcggagt gaacgagagc cggggatttc atcaccaaat tccgctgaag
     4621 ctgaggaatg gtaagatcat ctggcaggac gcgcagggag gggaactggc ctccgcgggg
     4681 gatatcgaag tgcatatcac tccgctcagg acgcagacgc gcgaaatgca gcacgcggtg
     4741 ttttcggcag ggcacgtacg ttcggccgcg ggggattaca tcacgggtat atttgtggag
     4801 cttctttcaa cacagggtca tcatgtgctg ctcgcccagc gcgccaaccg cacccgatcg
     4861 gatacgcctc cgtccggcgc gtaccagcag gcctatgaat acggtgtgcc ggattatctg
     4921 ccggatgacc cgcgcggcga gccggcgccc tgagccgctt gactttattt gcggactgtt
     4981 tatattcatc ttcgatttta ccggggccag cgtagctcag ctggcagagc aggtcattcg
     5041 taatgatcag gtcgatggtt cgaatccatt cgctggctcc atttatttga gccccggagc
     5101 ggtcatgccc gagaaaacgt tcaatgtgga tgatgtggca gaataccttc acattgcggc
     5161 gcgcgaaatt gaacgcctgg cccgtgaggg cgaaatccct tgcgcgcgca ggggatcccg
     5221 tcttgagttc cgccgccgtg aagtggacgc atgggccagc aggcgcatca tgaatttctc
     5281 cgggggagac ctcaccagct atcaccgcgt gtcatccgcc agaaaccacg acctgaacaa
     5341 gtcacatgcg atcatccctg aaatgatgcg tgtggattac gtggatgccg ccatgacctc
     5401 caagactcaa ccttcagtta tccgcgacat ggttgccctg gccggccgga ctggcctgct
     5461 ggtatatccg gaagacctgc tggagagtct ggtgaagcgc gagggcctcg ggtcaacagc
     5521 catgaccggc ggatttgcga ttctgcatcc gcagcaccac gaaccctata tgttcgagga
     5581 ttctttcatt gcgctgggcc gcacggtgca gccggtgccg ttttcatcgc cggacggcca
     5641 gcggacatcc atcttcttcc tgctttgctg ccaggacgac cggacgcatc tccacttgct
     5701 ggcgcgcatc tgcatgatgt gtcttcagac cagcctgatc atggaccttc agctggcggc
     5761 cgatgcgcag gagatgatga actgcatgat tgcaaccgag gagcagatca tccgcgggct
     5821 gtaaggcatg tgaggctgag tcgcgcgcgg actgaccgcc ctgccttaaa taatcacaga
     5881 ctcaccttcc agccagccac agtatccacc agttcatggc gggtcaggtc gaagccgatg
     5941 ggtgtcatgg agacatagcc cttggcaagc atgtcgaggt cggtatggtc gcgctctccg
     6001 gtgaactcca tgtctccgtc catccagaaa tagatgttcc cgcgcgggtc agcccgtttg
     6061 tcgaattttt caacatagcg ggagcgcgcc atgcgcgtgg cggcaaaacc cttgagctga
     6121 tcgtagggca ggttgggcac gttcacattc agcactgtgt cgcgcggaag cggatgcttt
     6181 tgaagaacgg gtatgagttt ctgcatcacg cgcgcgggtg tttcccagat gggatcggtg
     6241 aacgtgtcaa tggatatcgc cacgctctgc aggccgagaa tcgcgccctc ggtggcggcc
     6301 gagacagttc cggaataaat cgcagcgatg cccgcgttgg gcccgaggtt gattccgctg
     6361 attaccagat cgggcttgtg atccagcaga gcgcaaacag ccagcttgac gcagtcggcc
     6421 ggtgtgccgc tggtggcgtg gccgaacagc cggccgtcgc gctgaatggc caccgattta
     6481 atgggatcgg acagggtaat ggcatgtccc acggcgctcc gctgggaatc cggagccacc
     6541 acggacacgc ggcccatgtc cttcagggcc cgatgcagag cttcgatccc gggagcgtag
     6601 atgccgtcgt catttgtaag gagaatgttc atgggcgtga atgtggcacg ggccgcggag
     6661 tgaaacaagc gcgaaatgcg ccggccgtta aagagcgata aagcgagact tgtcatttcc
     6721 gggggcagca ggtacggtat gcaaggtcgt gctaggcggg gaatcagcgg tgccctgtac
     6781 ctgcaatccg ctacagcagg gttgaattcc cgagcgaggc ggcggtttcc tccggcacga
     6841 tccgcgaaaa ggcgctgagg gccgggccct gtgcagcaaa cgcggatgaa ccccgtcagg
     6901 gccggaaggc agcagcggta agtctggcgg tttgggtgcc gcaggtttac ctgaccggag
     6961 cccggccgcg gatacgaccg gtggaagacg ctgtcgaccg agggtgcacg accacttttt
     7021 tagcaggatt tgatgatatg gcgtatgaag ttctagcgcg gaaatggcgt ccgcagcagt
     7081 ttgacgatgt ggtcggtcag cggcacgtga ccgatacact caagaacgcc atcaaaaccg
     7141 gccgcattgc gcatgcctat ctttttgtcg ggccgcgcgg cattggcaag accaccagcg
     7201 cgcgcatctt tgccaaggcg ctgaactgcg aaaagggccc caccgccacg ccctgcgaca
     7261 agtgcgattc ctgcagggag atcatgtccg gccgcagcct cgatgtgatc gaaattgacg
     7321 gcgcctccaa ccgcggcatt gagcacatcc gcaacctgcg ggagaatgtc tgctatgccc
     7381 ccgcgcgcgg accgtacaag atttacatca ttgacgaagt tcacatgctc acatcggaat
     7441 ccttcaacgc gctgctcaag acgctggagg agccgccccc gcatgtgaaa tttgttttcg
     7501 ccacaaccga gccgcataaa atcctgccga ctatcctgtc acgctgccag cggttcgatc
     7561 tgcttccgat cccgaaccag gagatcatca cgcatctggg catgatcgcg aaggcggaga
     7621 agattgatat tgatgaagat gcgctgctgg ccattgcgcg cggcgccgag ggcggtttgc
     7681 gcgacgcgga gtcggccctc gatcagctcg tgtccttctg cggcgcaaaa atccgcgagc
     7741 cggatgtgat gtccgtgttc gggctggtgt cgcggcagat gctggaacgg ctggtggatg
     7801 caatgctggc tggggacatc tcggccgtgg tgaagcaggt ggccgaactt gcggattccg
     7861 gcaaggatat gctccgggtg gtccacgagc tgctggaata cttccggaac atcctcgtgt
     7921 atcacgcatc caagggctcc gaggagatgc tggggcttgg cgccgcgcag atggaagccg
     7981 tgaagcgcca gtcggaatct gccggaccgg agcggattct tcaaatcatt gagatcctga
     8041 ccgacacggc ctcccgtctc ggccgggcgc tttctcgccg caccctgctg gaaatcgcac
     8101 tgatccgctg ttcgcgagcc gccaccgcgg tctccatcga tgcgattctg tcgcaaa
//
LOCUS       DAOQWR010000121         8155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9281_length_8155_cov_6.065432, whole genome shotgun sequence.
ACCESSION   DAOQWR010000121 DAOQWR010000000
VERSION     DAOQWR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9281_length_8155_cov_6.065432"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKK36_09320"
     CDS             complement(<1..1166)
                     /locus_tag="PKK36_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR94790.1"
                     /translation="MNDRNKNRPGYKESKVGWIPMEWTEEPLGNVCTLIKKKVGNKKI
                     TPMSLSSGKGFVPQYEKFGKDISGKQYVNYTHLKRGYFTYNKGNSNRYPQGCAYLLKS
                     HDEVAVPNVFISFKLKETIANIGFFEQLFAANKHGRQLMKFINSGVRNDGLLNLTTEW
                     FLKVRLPLPLLPEQEKIAEILGCWDEGLESLEKLIAAKKLRKKGLMQQLLTGEKRLPG
                     FSKCWKEVSLGSISKAVKKAVGDKKVTPMSLSAGKGFVPQAEKFGRDISGKQYSKYTL
                     LTKGCFTYNKGNSIRYPQGCAYLLEDRDEVAVPNVFISFKLDEKKADNKFFQQIFAAN
                     MHGKDLVKFINSGVRNDGLLNLTTAWFFKIKFTIPSVDEQKAIAAVLETADEEIR"
     gene            complement(1163..2338)
                     /locus_tag="PKK36_09325"
     CDS             complement(1163..2338)
                     /locus_tag="PKK36_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR94791.1"
                     /translation="MKRPIQGETVVISTVGEKAVAFVPANLPPSPPIDWTPSLRNRFD
                     EALLALGRLDSVSLLLPDVSLFLYTYVRKEAVLSSMIEGTQSSLSDLLLFELNEAPGA
                     PFDDVSEVSNYVAALDHGLNRLREGFPLSLRLLREMHECLLKSGRGEHKTPGEFRRSQ
                     NWIGGTRPGNAVFVPPPPERVADCMGALEKFLHDDPEPTPVLLKAALAHVQFETIHPF
                     LDGNGRLGRLMITLLLCEAGVLKLPMLYLSLFFKTHRQAYYEFLNRVRIDGDWEIWLD
                     FFAQAVIETATQAVQTVQRLDALARKDREAIQSLGRAAGSALQLHHELLIRPITTSRA
                     LERATGLSHVSVNKALDHLCALGMAGELTGAKRNRIFSYTEYIRILNEGTEPPGESE"
     gene            complement(2445..3947)
                     /locus_tag="PKK36_09330"
     CDS             complement(2445..3947)
                     /locus_tag="PKK36_09330"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNR94792.1"
                     /translation="MNDQQIKSDIFNVVWKACDTFRGTIDPSTYKDYILTMLFVKYLS
                     DVRKTKLAEYEKKYNGDKVRIERDMRLERFCIAEDCTFDRLFARREDPEIGQIINTVL
                     ENIEDTNKAKLHNVFRNIDFNSEANLGKARQRNERLKNLLQDFNDPKLDLRKERVGHL
                     DIIGDVYEYLIAKFASQAGKKAGEFYTPAGVSETLARLVAPQKGDRICDPTCGSGSLL
                     IKASKQVGSNDFSLFGQEMNGATWALCKMNMFLHEVDSARIELEDTIREPLLLEGNSL
                     MKFDIVVANPPFSLDKWGQEMAATDKYERFSRGIPPKSKGDWAFIMHMLATAVEGKGR
                     VGVVVPHGVLFRGDAEGRIRRAVIEENLLAGVIGLPANLFFGAGIPAALLIFDKSRKP
                     GGKENVFFIDASREYEQGTNQNVLRQQDMAKIVETFHARKVIEKYSYSATFAEMEEND
                     FNLNIPRYVDTFEPEPEVDIPAVQETIQGLEEKLAGVRKKMDGYLKELGF"
     gene            complement(3940..4557)
                     /locus_tag="PKK36_09335"
     CDS             complement(3940..4557)
                     /locus_tag="PKK36_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR94793.1"
                     /translation="MNIQVQSESVSNLAEISAGYQLRFRPSEDRNGRVLLIQPRNINA
                     ERTAVNFNEALRFEPARNYSKSLLKEGDVLFMGKGTTPFACPVYNMPSRSIAAGNFFI
                     LRPDEKRILPEYLAWMLNRKKIRETLLIASGAGVLMPVVRKKDLEDLNIPLPPLEMQK
                     KIIELHRLMAEEKFLMRELAEQKELLIRGVCKKLTGESEHGENNE"
     gene            complement(4667..5203)
                     /locus_tag="PKK36_09340"
     CDS             complement(4667..5203)
                     /locus_tag="PKK36_09340"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNR94794.1"
                     /translation="MRGKTVAVFGSAIADPADEACRQAVLAGRIIAEAGFNILCGGYA
                     GVMEAACRGCTEAGGKCAGIGLTKFTAQPNKYITRFKAVETLGRRLDYFDKHSCAFLA
                     LDGGIGTVTEVMFFWDIAKAGFLEGRPIFLLGEQWPRLVQELQERFIIKDKYMSCVQC
                     LPDLSELKSALRVLEQPR"
     gene            complement(5185..5481)
                     /locus_tag="PKK36_09345"
     CDS             complement(5185..5481)
                     /locus_tag="PKK36_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94795.1"
                     /translation="MKNVQKTLGLSIHEYCAEDDTRSVRGKFPEPVDLHALKTAAEKI
                     STKVMLMERLQALVIPLNQGVVHIHANGDIVINRIKRLEDAEEFMEKLLCAEKQ"
     gene            complement(5478..6140)
                     /gene="pyrF"
                     /locus_tag="PKK36_09350"
     CDS             complement(5478..6140)
                     /gene="pyrF"
                     /locus_tag="PKK36_09350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNR94796.1"
                     /translation="MWKQHSGIMLALDVENRAQAFRLLDLIGEDCDAIKFNYPLVLRE
                     GLSIVTEVKNRCKKPILCDFKVADVPVTNDRIIRICGEAGADGVMVHGFIGMDALQSA
                     VQVAGNTALFLVTQMTNPGGLDFTAQFTDEFAAMARTFGLAGVQAPGNRPEVVRHVRK
                     IIGPDMMIVCCGIGAQGGKFGAAIEAGATFEIIGRAIYDAKDPVAKVAEIKAATRPFI
                     KS"
     gene            complement(6124..7302)
                     /locus_tag="PKK36_09355"
     CDS             complement(6124..7302)
                     /locus_tag="PKK36_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR94797.1"
                     /translation="MEKRPKTYLDWAATSWPKPETVYDAVNRYAHNVGIDHNRGHFRE
                     AQEAEAWFGRARAAIAALLKAPAPERILFFSGATEALNTALLGTLKAGDHVIFSSFEH
                     NAVVRPLRWLEKHRGVMLAQIPGSVSEPLDPSAVRRAIRKDTRMVVLNHISNVFGSVL
                     PVCEVGKILEEYDDILFVVDGAQSLGSHPVDVRAMHADIFTFPGHKSLLGPPGTGGFY
                     LREGLEKIVSPVKFGGTGVKSATEIVVEELPHKYEVGTQNSWGIAGLAAGAEFVLARG
                     VEDISAQLAQLTSELVNGLRENSLIRLYAPPPGIHHGVVSFNFSNMPSEDVAALLDRS
                     HNIKLRAGIHCSPDAHRHTNTLRQGTVRASFGALSADGDAESLIRALREMREDYVEAT
                     "
     gene            7412..7993
                     /locus_tag="PKK36_09360"
     CDS             7412..7993
                     /locus_tag="PKK36_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNR94798.1"
                     /translation="MSEDKLFLSASDLLTMSYRLARMIWDDGFIPTHLIGLWRGGTPI
                     AIAIHEFFATAGHDCFHMPVKTRRSLQPDGSYHISIVGESHFLENVHRDSRILFVDDI
                     FDTGLTMDSLIRMTAAQKGFLPEIRTATIFYKPAKNRTSITPDWFIHKTDAWIIFPHE
                     ISKLTPEELHENKPFLWPYIEDILNPGGPGDGK"
     gene            7983..>8155
                     /locus_tag="PKK36_09365"
     CDS             7983..>8155
                     /locus_tag="PKK36_09365"
                     /inference="COORDINATES: protein motif:HMM:NF026032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNR94799.1"
                     /translation="MENNVIILKHPLIEHKLAHLRNRESDNKLFRETLSELSYLLVYE
                     ITRDLKLKEVPIET"
BASE COUNT         1708 a         2325 c         2167 g         1955 t
ORIGIN      
        1 cgaatctctt catccgccgt ctccaaaacc gccgcaatcg ccttctgctc atcgacggaa
       61 ggaatcgtga atttgatttt gaaaaaccac gctgtggtta agttaagcag gccatcgttc
      121 cgtacgccac tgttgatgaa tttcactaaa tccttgccgt gcatatttgc tgcaaagatc
      181 tgttggaaga atttgttatc cgcttttttc tcgtcgagtt taaagctgat aaaaacattc
      241 gggaccgcaa cttcatcacg atcttcaagt aaataggcgc atccttgcgg ataccggata
      301 gagtttccct tattgtaggt aaaacagccc ttcgtcagca gagtgtattt cgagtattgc
      361 ttgcccgaaa tatcccgtcc aaacttttca gcctgcggaa caaatccttt tccggcagaa
      421 aggctcatag gggtaacttt tttgtcgcca acagctttct tcacggcctt actgatgctt
      481 ccaagagaga cctctttcca acacttggag aaacccggca accgcttttc accagtcagc
      541 aactgttgca tcagcccctt tttgcgcagc tttttcgccg caatcagctt ctccagactc
      601 tccagcccct catcccagca ccccaaaatc tccgcaatct tttcctgttc cggaagaagg
      661 gggaggggga gcctaacttt taaaaaccac tctgttgtaa ggtttagtaa cccatcattt
      721 cgcacgccgc tattgatgaa tttcatcagt tgtcttccgt gcttgtttgc tgcgaagagt
      781 tgttcgaaaa agccaatatt tgcaatggtc tctttcagct taaagctgat gaaaacattt
      841 ggaacggcta cttcgtcatg gcttttaagg agataggcgc atccttgtgg gtaacgatta
      901 gaattacctt tgttgtaagt aaaatatccg cgcttgagat gcgtgtagtt cacatactgc
      961 ttaccggaaa tatctttgcc aaatttctca tattgcggca caaagccttt gccggaggag
     1021 agactcattg gtgttatttt tttattacca actttctttt tgattaaggt gcaaacattc
     1081 cctaggggtt cctctgtcca ctccatcgga atccatccga ctttgctttc cttatagccc
     1141 ggtctgtttt tgttgcggtc gttcattcgg attctccggg cggttcggtg ccttcgttca
     1201 gaatgcggat atattcggtg tagctgaaga tcctgtttcg tttggcaccg gtcaattcgc
     1261 cggccatgcc aagcgcgcaa aggtgatcga gcgctttgtt gacgctgacg tggctcagtc
     1321 cggtggcgcg ttccagggcg cgggaggtgg taatggggcg gatcagcagt tcgtggtgca
     1381 gttgcagcgc ggagccggcg gcccgcccaa gggactggat cgcctcgcga tccttcctcg
     1441 ccagtgcgtc cagccgttgt acggtttgca cggcctgtgt ggcggtttcg atcacggcct
     1501 gggcaaaaaa atcgagccag atctcccagt cgccatctat tcgaacgcgg ttcaagaact
     1561 cgtagtaggc ctgccgatgg gttttgaaga agaggctgag gtagagcatg ggaagcttca
     1621 gaacgcccgc ttcgcacagc aggagcgtaa tcatcagccg cccgagtcgt ccgttgccgt
     1681 cgagaaaggg gtggatggtt tcaaattgca catgtgccag cgcggctttc agcagaaccg
     1741 gagttggctc agggtcgtcg tgcaggaatt tttccaacgc gcccatgcag tcggcaaccc
     1801 gttccggcgg aggcggaaca aagacggcat tcccggggcg ggtgccgccg atccagttct
     1861 gactgcgccg gaattccccc ggcgtcttgt gttcgccccg ccccgacttc agcaggcatt
     1921 catgcatctc ccgcaaaagg cggagcgaaa gcgggaatcc ctcacgaagc cggttcaggc
     1981 cgtggtccag tgccgcgaca tagttgctga cttcgctgac atcatcgaag ggggcacctg
     2041 gtgcttcgtt caattcgaaa agcagcaaat cggacaggga agattgggtt ccctcaatca
     2101 tcgaagagag aacggcttcc ttgcgcacat aggtgtagag gaagagggat acatccggca
     2161 gcagaaggct tacgctgtcc agccgtccca gtgccagcaa ggcttcatcg aaacggttgc
     2221 gaagcgaggg agtccagtca atgggcggtg atggcggaag attcgccggg acaaacgcca
     2281 ctgctttttc acccaccgtg gatatgacca cggtttcacc ctggatgggc cgtttcataa
     2341 gtttcccttg ctgacattac gaattcctat atgtaaggat atgacaataa gattacatat
     2401 agattgttgt atgtaaagaa aactctttgt ttttgtacac atcatcaaaa tcccagttcc
     2461 ttgagatacc catccatttt cttccgcacc ccggccagtt tttcttccaa cccctggatc
     2521 gtttcctgca cggcgggaat atccacttcc ggctcgggct caaaggtgtc cacatagcgc
     2581 ggaatgttca ggttgaagtc gttctcctcc atctcggcaa aggtggcaga atagctgtat
     2641 ttctcgatca ccttgcgcgc gtggaacgtc tcgacaattt tggccatatc ctgctggcga
     2701 agcacattct ggttcgtccc ctgttcatat tcgcgggagg catcaataaa gaagacattt
     2761 tctttgccgc ccggcttgcg tgacttgtcg aagatcagca gcgccgccgg aatgcccgcc
     2821 ccgaaaaaca gattggcggg cagtccgatg acgcccgcca gcaggttttc ctcaatcacg
     2881 gcacgccgga tgcggccttc cgcatcaccg cggaacagca cgccgtgcgg gaccaccaca
     2941 ccaacgcgac ctttgccctc caccgctgtt gccagcatat gcatgataaa cgcccagtca
     3001 cccttgcttt tcggcggtat gccccgactg aaccgctcat acttgtccgt cgccgccatt
     3061 tcctgacccc atttgtcgag cgagaagggc gggttcgcca ccacaatgtc aaatttcatc
     3121 aggctgttgc cttcaagcag cagcggctcg cggatcgtgt cttccagttc aatgcgggca
     3181 ctgtccactt cgtgcaggaa catattcatt ttgcacagcg cccaggtcgc gccgttcatc
     3241 tcctgcccga agagcgaaaa atcgttgctt cccacctgct tcgacgcttt gatcagcagc
     3301 gaacctgagc cgcaggtcgg atcgcagatg cggtcgcctt tctgcggggc caccagtcgc
     3361 gccagagttt ccgacactcc agcgggcgtg tagaactcac ccgctttttt gcccgcctgc
     3421 gacgcaaact tggcaataag gtattcatag acgtccccga tgatgtccag atggccgacc
     3481 cgttctttgc gcaggtcgag tttcgggtcg ttgaaatcct gcaacagatt tttgagccgt
     3541 tcattgcgct gccgggcttt gcccagattg gcttcactgt tgaagtcaat gttgcggaat
     3601 acattgtgca gcttggcttt gttggtatct tcaatatttt cgagcacggt gttgatgatc
     3661 tgccctattt ccgggtcttc ccgcctggcg aagaggcggt caaacgtgca gtcctcggcg
     3721 atgcagaacc gctcaagccg catgtcgcgt tctatgcgca ccttgtcgcc gttatacttc
     3781 ttctcgtact ccgccagctt tgttttccgc acgtcgctca aatattttac aaacagcatg
     3841 gtcaggatgt agtccttata ggtgcttggg tctatcgttc cccgaaaagt gtcacaagcc
     3901 ttccatacca cattgaaaat atcgcttttg atctgctggt cattcattgt tttctccgtg
     3961 ttcagattct ccggtgagtt ttttgcagac accgcggatc agcaattctt tttgttccgc
     4021 cagttcgcgc attaaaaatt tttcttcagc catcagccgg tgcagttcaa taatcttttt
     4081 ctgcatttcc agcgggggca gggggatatt caaatcttcc agatcttttt tccgcaccac
     4141 tggcattaag actccggctc ctgacgcaat cagcagggtt tctctgattt tcttccgatt
     4201 tagcatccag gcgagatatt ccggaagaat tcgcttttcg tcgggcctca gaataaagaa
     4261 attgcctgca gcaattgacc gggagggcat attgtaaaca gggcaagcaa agggtgttgt
     4321 tcctttaccc ataaacagaa catcaccctc ttttagcagc gacttcgaat aattccgggc
     4381 tggctcgaat ctaagggctt cattaaaatt gaccgctgtt ctttcggcgt ttatgtttct
     4441 tggctgaatc agcaaaaccc gtccgtttct atcttcgctt ggacggaaac gaagctggta
     4501 cccggccgat atttcggcca ggttcgacac cgactcgctt tgaacttgaa tattcatatt
     4561 aacagtattt aactatctgt ggttataaat acagaaaatc agtggtcgcg tcaagcaaaa
     4621 atgatattta ttctaacagt atatcaaata ccgttataat atttaatcat cttggctgtt
     4681 ctaataccct aagggcactt ttcagctcgg aaaggtccgg caggcactgg acgcaggaca
     4741 tgtatttgtc ttttatgatg aagcgctctt gaagttcctg gaccagccgc ggccattgtt
     4801 cgcccagaag aaaaatcggg cggccctcaa ggaagccggc cttggcaatg tcccagaaga
     4861 acatgacttc cgtgacggtg ccgatcccgc cgtccagcgc gaggaaggcg cacgagtgct
     4921 tatcaaaata gtccagccgc ctgccgaggg tttcaaccgc cttgaaccgg gtgatgtatt
     4981 tgttgggctg cgcggtgaat tttgtcaggc cgatgcccgc gcattttccg ccggcctctg
     5041 tgcacccgcg gcaggccgcc tccatgacac cggcgtagcc gccgcacaga atgttgaagc
     5101 ccgcctcggc gatgatccgc ccggccagca ccgcctggcg gcaggcctcg tcggccggat
     5161 ccgcgatcgc gctcccgaaa accgctactg tttttccgcg cataacagct tctccatgaa
     5221 ttcctctgcg tcctcgagcc gcttgatgcg gttgatgacg atatcgccgt tggcgtgaat
     5281 gtgaaccacg ccctgattga gcggaatcac gagcgcctgc agccgctcca tgagcataac
     5341 cttcgtggaa atcttttccg ccgccgtctt gagggcatgc agatccaccg gttccgggaa
     5401 ttttccgcgg acggaacgcg tgtcgtcctc cgcgcagtat tcgtggatgc tcaggccgag
     5461 agttttctgg acatttttca tgacttgatg aacgggcgtg tggcggcttt gatctccgcc
     5521 accttggcca caggatcttt tgcgtcgtag atggcccgtc cgatgatttc gaacgttgcc
     5581 ccagcttcga tcgcggcgcc gaacttcccg ccctgcgctc cgatgccgca gcagacgatc
     5641 atcatgtccg gcccgatgat tttccgcaca tgccgcacga cctcgggtcg gttccccggg
     5701 gcctgcactc cggccagccc gaaagtgcgg gccatggcgg cgaactcgtc cgtgaactgc
     5761 gccgtgaaat caagcccgcc gggattggtc atctgggtca cgaggaagag agcggtattc
     5821 cccgccacct gaacggccga ctgcagggcg tccatgccga taaatccatg gaccatgacg
     5881 ccgtccgctc cggcctcgcc gcagattctg atgatgcggt cgttggtgac gggaacgtcg
     5941 gccaccttga aatcgcagag gatgggcttc ttgcagcggt tcttcacctc ggtgacgatc
     6001 gaaagcccct cgcgcaggac cagggggtaa ttgaacttga tggcatcgca gtcctcgcca
     6061 atgaggtcga ggagcctgaa cgcctgcgcg cggttttcca cgtccagcgc cagcatgatt
     6121 ccgctatgtt gcttccacat agtcttccct catttcgcgg agcgcccgga tcaaagattc
     6181 ggcgtcgccg tccgcgctca gcgcgccaaa actggcgcgg acggttccct gacggagcgt
     6241 attggtatgc ctgtgcgcgt ccggcgaaca atgaatgccg gcgcggagct tgatgttgtg
     6301 ggaccggtcc agaagcgcgg ccacgtcttc cgaaggcatg ttgctgaagt tgaatgaaac
     6361 cacgccgtga tgaattcccg gcgggggggc atacaaccgg atcagcgaat tctcccgcag
     6421 cccgttcacc aactcgctgg tgagctgcgc gagctgggcg ctgatgtctt ccacgccgcg
     6481 cgccagcaca aattccgcgc ccgccgccag cccggcgatg ccccatgagt tctgagtgcc
     6541 gacttcatat ttatgcggca gttcctccac cacaatttcc gtcgcggatt ttactccggt
     6601 gcccccgaat ttgacgggag aaacgatttt ttcaagaccc tcgcggagat agaagccgcc
     6661 ggttccgggc ggtcccagca ggcttttgtg gccggggaac gtgaagatgt ccgcgtgcat
     6721 ggcccggaca tccaccgggt ggcttccgag gctctgcgcg ccgtccacca caaacagaat
     6781 atcatcgtat tcttccagta ttttcccgac ctcgcagacg ggcagcaccg agccaaagac
     6841 attggagata tggttcagca ccaccatgcg cgtgtccttg cggatggccc tgcggacggc
     6901 ggaggggtcg agcggctcgg acacagaacc cggaatctgc gcaagcatga caccccgatg
     6961 cttctccagc cagcgaagcg gccgcaccac cgcattgtgc tcaaaggagg aaaagatgac
     7021 gtggtcgccc gccttcagcg tgcccagcag cgccgtgttg agggcttcgg tcgcgccgga
     7081 aaagaaaaga atccgttccg gcgccggcgc cttcagcagc gcggcgattg cggcgcgcgc
     7141 gcgcccgaac cacgcctcgg cctcctgcgc ctcgcggaaa tggccgcgat tgtggtcaat
     7201 gccgacattg tgcgcgtagc ggttgacggc gtcatagacg gtttccggct tgggccagga
     7261 ggtggccgcc cagtccaaat aggttttggg tcgcttttcc atgtcaccaa gatagcacac
     7321 cggggcggct gggagcaaag atttgtgcgc gttcgatatt ccattgcgct tcggcggcaa
     7381 acagatatga tggtttcggg acgaagttgc gatgagtgag gacaagctgt ttttaagcgc
     7441 cagcgatttg ctgaccatgt cgtaccggct tgcgcggatg atatgggatg acggcttcat
     7501 ccccacgcac ctgatcggcc tgtggcgggg gggcacgccg atcgccatcg cgattcatga
     7561 attctttgcg acggccgggc atgactgctt tcacatgccg gtgaaaacac gccggtcgct
     7621 tcagccggac ggcagctatc acatctccat tgtcggcgag tcgcactttc ttgagaacgt
     7681 gcaccgtgac agccgcatcc tttttgtgga cgacattttt gacacaggtc tcacgatgga
     7741 ctcgctcatc cggatgacgg ctgcgcagaa gggattcctt ccggaaatcc ggacagcgac
     7801 catattctac aagcccgcga aaaaccggac atccatcacg ccggactggt tcatccataa
     7861 aacggatgcg tggatcattt tcccgcatga aatcagcaag ctcacgccgg aggaactgca
     7921 cgagaacaag ccgttcctct ggccgtacat tgaagacatc ctgaatcccg gaggacccgg
     7981 cgatggaaaa taatgtgatc atccttaagc atccgctcat cgagcacaag ctggcgcatt
     8041 tgcgcaaccg cgaatccgac aacaagctct tccgggaaac gctcagcgag ctgtcctatc
     8101 tgctggtcta tgaaatcacg cgcgacctga agctcaagga ggtccccatc gaaac
//
LOCUS       DAOQWR010000122         8140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9306_length_8140_cov_5.444280, whole genome shotgun sequence.
ACCESSION   DAOQWR010000122 DAOQWR010000000
VERSION     DAOQWR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9306_length_8140_cov_5.444280"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1408)
                     /locus_tag="PKK36_09370"
     CDS             complement(29..1408)
                     /locus_tag="PKK36_09370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR94800.1"
                     /translation="MKSIALQFGLCAAILLAAGSAHGAVYADFETPHGTFTVELDVLN
                     APRATANFLGLADGSQTWRDPVTGAVRGGEAGDSSFYDGMRFYATAGQAALLGGLRSY
                     TGTTGTEYWEGPGYTILDEVTNGVDLARGVLAMPEFEGPHSGGGELAVMLTNDAYLGS
                     GWTAFGMVTGAGMAVVDAIVSEVTNADTRVAAQITIRDAGITPQETQALAVARNDLPV
                     MEEMPLGLNREGDGTRTVLFWSLPQSQACLAVSSNLMKDGWSVMPGNWNSDTNQAWMS
                     VPLAYVPGLVDGLGFLYGSQAVYPKMTASLFSDKCRIAVAHTGVDYQYWLDFAGGTGI
                     WARVEDGAPVESGSISSLGQELARANSVHLVLVIGLNAYHYWFGFDEAGTSDGRFYCE
                     IRYLNSPPPTATDSGTFIYEDGWGAKVPAMKNRRRSSRTKTAARAPADRKSTLLLNNE
                     WPAPRRLRR"
     gene            complement(1528..2154)
                     /locus_tag="PKK36_09375"
     CDS             complement(1528..2154)
                     /locus_tag="PKK36_09375"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNR94801.1"
                     /translation="MKKVLVALVVLSMASVASAELLASWVTGDASGAVTAGTNADVVV
                     SGLSLWGDATSTGTTSVWGMRTLTTGGGLQFTISSIADGQRIEDAVISGTASGSGTGP
                     REMDWFVDGSIVSGQSIVRTSTTATAFENTLGTLNSGDVVSLRADTAAGTVRSGTLEN
                     FTNTAGTFYITAAGMELNGNVVIPEPGTMALLGLGGLAILAVRRKMLK"
     gene            2390..3142
                     /locus_tag="PKK36_09380"
     CDS             2390..3142
                     /locus_tag="PKK36_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR94802.1"
                     /translation="MLNGKKIVVVMPAYNAAKTLQKTYQEVQALGCVDRIIVVDDASQ
                     DGTAALAQALPGALVHVHEQNLGYGGNQKTCYQLALAEGADIIIMVHPDYQYTPQLIP
                     AMASMIACGLYECVLGSRILGGGALTGGMPLWKYISNRLLTFSENILLGTKLSEFHTG
                     YRAFSRSLLERLPLEKNSNDFVFDNEMLAQIAWLGVFIAEVSCPTKYFPEASSINFRR
                     SVRYGLGCLRVGCFFRLARWGLTRFKSLMPSA"
     gene            complement(3178..5523)
                     /locus_tag="PKK36_09385"
     CDS             complement(3178..5523)
                     /locus_tag="PKK36_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNR94803.1"
                     /translation="MIGEQAPSGSLFCVVTAMKMLSRSEMRYAGLLLLLLVLAAVACY
                     ETLSYLEDRILTLDKQRAENRLSDYLTFEQDRMMAVLEHAMESRPPLDASQSPFLQKI
                     IESGQIMQINLLAAAIVAPDGSLLDVVLSEGSRGRVDDGGQSLLDWKSFSEAVRDVRM
                     TSAGYAGSALGPAFLAFSPIPAYDGQALLLILRALDREYVRKIRQTYPDLEGMTINSE
                     AAAPDQRIAIYDYSGVPVGYFTVSRESSLSGAVISSIGLPVYLLIMSFLFLSGGLGLW
                     AIRFSTVIEGRRRVGRFVDAMDYLPDGVLLVDRATRAEGINPAAIEMSGGLARKGTAL
                     EVIFPFLSAEDVNTLMVTSGPHEVEKQFASAGGPHVWRFRSQPMEGMALIMISDVTSR
                     RAKELRERQIATIHMIGRIARGVAHDFNNILCAVSGHAELLRALKPGREEMESVSAII
                     GESDRGARLAAHLLELSRMGDGGQPAEQVAEAVRQAGEILRVGLSYGWDIRVECGANA
                     FPPVMLSRIQLEQVVVNLGLLVADDMGTEGVLHVMLRHPSEECLLKVSQSCAALVMVW
                     ASHVHAPVLEMDTILEEAPATQYGGVIHSVLRSMLEEVDGRLDTFQYGRRFLYRVCLP
                     GAPAHVDPHKNGIPQSGEGNLYKPADDYLRGRKIMLASTSFANIADLHVLLMERGGEI
                     RVAGSLVEALDIVNERNPLHLIVYDMGMLGEDARGLLRATLKLHPQAGILALCREEDD
                     EVSALADRVEFSPHEASAPELLEALSSAWEKARRGIGSSMR"
     gene            complement(5583..>8140)
                     /locus_tag="PKK36_09390"
     CDS             complement(5583..>8140)
                     /locus_tag="PKK36_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94804.1"
                     /translation="SSLQIYAGYVLPGGQKRILQGPSVTTTGGWTHIAASYDALNGTL
                     TIYTNGVPALSGTGFHEAPPLNGRGGATFVRIGEDYAGLIDDVRIWGDARTDAEIRKY
                     YYDYVTTGEDLVHYFRFDDGQASDTNGIAFGDYHRQEGAEDYVFAKDWNQQWMHAARL
                     NGSVSFTTNGAIEPPPSVRIILQPDAAVLAGAQWSIDNGPWMDSGVTVSDLDRFDMTH
                     TVTYRSILGWSAPDNETITVTNGETLVLNRSYVQRGSLTVTIQPLEVNEAAWFFDSQY
                     GGYLWSIGNIGTNGARRDDMIWQLNGTTIENLEVGTYTLRFYPVPGWNTPVSREIVIS
                     EGAAVSAVGHYTDVEGYVRGIIEPPEVIADGAMWRVDNGPWRPSDTISGVLDYGAHQI
                     EFLNVPPWITPGPVMLVITNNGVFDVPGLYQEVSGIYVELHPDEAIALGARWTLNGTE
                     YESGTLVPLAAGDYGVTFTQLDGWLSPSEQLVTVFSNQTTELDGYYLRTELLMDDVPG
                     LLNEPRGLALDSQRRLYIADSGNDRIMVLDTRDGTTTNLGSPGTSSGQFNQPVGVAVD
                     GADNLYVADAANNRVQRRNSVTGTWTVWGGTASGTALGQFKVPYDVEVDALTNLYVAD
                     RDNHRVQRMSPGGVWTAFASNGYAVGMVRTPIGLTVDRSNLVYVADYPDNSNGRVQRF
                     TTNGLAARIGSYLPSEGTLLKTRGMTFGRSNDLYAADMMTSEINVGSDSGQWATLVSS
                     GLLHGPEDVEWDPRGYLYICDTLSNRILRVEIGLAGNIPPAVASTTIDPSGITINWEG
                     AEGWFYSIQYSDDSPAGPYVELPGAEAIPGEGGVMSYTDTTADGMRFYRILAY"
BASE COUNT         1750 a         2567 c         2141 g         1682 t
ORIGIN      
        1 cccggcttct ttatgccccg tgatcaggtt accggcgcaa acggcgcggc gcaggccatt
       61 cattgttcag caagagcgtt gactttctgt ctgctggtgc gcgagccgcc gttttcgtcc
      121 ttgaactccg tctccggttt ttcatcgccg gcaccttcgc gccccatccg tcttcataga
      181 taaacgtacc gctgtccgtt gctgtcggtg ggggggaatt caaatacctt atctcgcaat
      241 agaatcggcc atcggaagtc ccagcttcat caaaaccaaa ccagtagtga taagcattca
      301 agccaataac caataccaga tgtacgctgt tggccctcgc cagttcctgc cctaaactgg
      361 aaatgcttcc ggattccacc ggcgcaccat cctccactcg ggcccagatc ccggtccccc
      421 ctgcaaaatc aagccagtac tgatagtcca ctcccgtgtg cgccacggcg attctgcact
      481 tatcagaaaa cagactggcc gtcattttcg gataaaccgc ctgtgaaccg taaaggaaac
      541 ccagcccgtc cacgagaccg ggaacatacg ccagtgggac actcatccat gcctgattgg
      601 tatcgctgtt ccagttgccc ggcatgacag accagccatc cttcatcaaa ttagatgaga
      661 cggcaaggca ggcctgactt tgcgggagcg accagaacaa aaccgtgcga gtcccgtcgc
      721 cttcgcgatt caatcccagc ggcatctctt ccataaccgg aaggtcgttt ctggccacgg
      781 ccagcgcctg ggtttcctgg ggagtgattc cggcatcgcg gattgtgatt tgggcggcca
      841 cacgggtgtc ggcatttgtc acctcgctca cgatggcatc caccacggcc attcccgcac
      901 cggtcaccat tccgaacgca gtccatccgc tgcccagata agcatcattg gtcagcatca
      961 cggccagttc tccgccaccg ctgtgcgggc cttcaaattc cggcatggcc agaacaccgc
     1021 gggccagatc cacgccattg gtcacttcat ccagaatggt atatcccggg ccttcccagt
     1081 attcagtccc cgtggtgccg gtataggagc gcaatccccc cagcaaagca gcctgtcctg
     1141 ccgttgcata gaagcgcatt ccatcataga aggaggaatc cccggcctct ccgcccctga
     1201 cagctcccgt cacgggatcc cgccaggttt gtgaaccatc cgccaggccc agaaaattgg
     1261 ccgtggcgcg aggggcgttc agcacgtcca gttccaccgt gaacgttcca tgaggggttt
     1321 caaaatcggc gtaaaccgca ccatgcgcgg aaccggccgc cagcagaatg gccgcgcaca
     1381 atccaaattg cagagcaatt gatttcattg ctttaatcta cagcctcttc cgcattccgc
     1441 aagccgaaac agacagatct gggccgtgtt ctgcccaaaa gaaaaacccc gcatttctgc
     1501 ggggtttttc gtttctatct gttgaaatta tttcagcatc ttgcgacgca cggccagaat
     1561 ggccagtccg cccagaccca gcagcgccat ggttcccggc tccgggatca ccacattgcc
     1621 gttcaactcc atgccggccg cggtaatata gaacgtgccc gcggtgttgg tgaagttctc
     1681 aagagttccg gaacgcacag tgccagccgc cgtatcagcg cgcagggaaa ccacatcgcc
     1741 gctgttcagc gtgcccaggg tgttctcaaa cgctgtcgcg gtcgttgacg tccgcacaat
     1801 gctctggccg gaaacgatgc tgccgtccac gaaccagtcc atttcacggg gaccggttcc
     1861 ggagccggaa gcggttccgg agataacggc atcctcaatc cgctgtccgt ccgcgatgga
     1921 cgaaatcgtg aactgcaggc cgccgcccgt tgtcagtgtc cgcatgcccc acacagacgt
     1981 tgtgcctgtg ctcgttgcat cgccccacag ggacagaccc gacaccacga catcggcgtt
     2041 tgttccagcc gtcacagcgc cggacgcatc acccgtcacc cacgaggcca gcagttcggc
     2101 acttgccacg ctcgccatgc tcaacaccac caatgctacc aataccttct tcatttcttc
     2161 tctcctcttt ggttaaccaa atatccagat ttcaaccact ttcttgaagc caatctaccg
     2221 gaacagaaat gctttgtaaa cttttttttt cattattttt gaactttttt taaggccgcg
     2281 tttcccttta ttttccggca ttttgtgaga attcgcaggc atcagcggtt ttttacaatg
     2341 ccattcgatt atattttgaa ttccatgaaa aaaatggcat aaagggacca tgctgaacgg
     2401 gaaaaaaatc gtggtggtca tgcccgcata caatgcggcg aaaacacttc aaaaaactta
     2461 tcaggaggtg caggccctcg gatgcgttga ccgcattatt gtggtggacg acgccagcca
     2521 ggacggcacc gctgcgctgg cgcaggccct gcccggagcg ctggtccatg tacatgagca
     2581 gaacctggga tatgggggaa atcagaaaac ctgctaccag ctcgctcttg ccgaaggcgc
     2641 cgacatcatc atcatggttc atcctgatta ccagtacacc cctcagctga ttccggccat
     2701 ggcgtccatg atcgcgtgcg gactttatga atgcgtgctg gggtcgcgca ttctgggcgg
     2761 aggagctttg accggcggca tgcccttgtg gaaatatatt tccaaccgcc tgctgacttt
     2821 ttcagaaaat attctgctgg gcacgaagct gtccgaattc cacaccgggt accgggcctt
     2881 ctcgcgcagc ctgcttgagc ggctccctct ggagaaaaat tcaaatgact tcgtgttcga
     2941 caatgaaatg ctggcgcaga tcgcatggct cggagttttc attgctgaag tgagctgtcc
     3001 cacaaaatat tttcccgaag cgtcttcgat caatttccgg cgcagtgtgc gctatggtct
     3061 gggctgcctg cgggtgggat gctttttccg gctggcccgg tggggcctga cccggttcaa
     3121 gtccctcatg ccttccgcct gaacaaccgc ggcggcgcgg agaatttgcg acggcaccta
     3181 ccgcatggaa gatccaattc cgcggcgggc tttttcccat gcgctggaga gcgcttccag
     3241 aagttccggt gcggaagcct catgagggct gaactccaca cgatcagcca gggcagacac
     3301 ctcatcgtct tcttccctgc acagagcaag aatgcccgcc tgcggatgaa gcttcagtgt
     3361 ggcgcgcaga agcccccgtg cgtcctcccc cagcattccc atgtcatata caatcaggtg
     3421 cagagggttt ctttcattca ctatatccag cgcttccacc agactcccgg ccacgcggat
     3481 ttcgccaccc ctctccatca gcagcacatg cagatcggcg atgttcgcga atgaagtgga
     3541 ggccagcatg atctttcggc cccgcagata atcgtctgcc ggcttgtaca gatttccttc
     3601 tccggactgc gggatgccgt ttttatgcgg atcaacatgt gcgggcgcgc ctggcagaca
     3661 aacccgatag agaaacctgc ggccatactg aaaggtatca aggcgaccgt ccacctcctc
     3721 cagcatactg cgaagcacgg aatgaataac gccgccgtac tgagtcgcag gggcttcctc
     3781 cagtatcgta tccatttcca gcacgggggc gtgaacatgc gatgcccaca ccataaccag
     3841 cgccgcgcag ctctgcgaaa ccttgagaag acattcctcc gatggatgcc tgagcatgac
     3901 gtgcaaaaca ccttccgtgc ccatatcatc cgccaccagc agccccagat tcacaaccac
     3961 ctgctccagc tgaatccggg aaagcatgac aggcggaaac gcgttcgcgc cgcattcgac
     4021 ccggatgtcc cagccatacg aaagccccac gcgcaggatt tcaccggcct gacggaccgc
     4081 ctccgcaacc tgctcggccg gctgcccgcc gtcccccatc cggctgagtt ccagcaggtg
     4141 ggccgccaga cgcgcacccc ggtcagactc tccgataatt gctgaaaccg actccatctc
     4201 ctcccgcccc ggcttcaaag cgcgcagcag ttctgcatgg cctgaaaccg cgcacagaat
     4261 attgttgaag tcgtgggcca cgccgcgcgc aatgcggcca atcatgtgaa tagtggcgat
     4321 ctgccgttcc cggagctcct ttgccctgcg gctggtcaca tcgctgatca tgatgagcgc
     4381 catgccctcc atgggctggg agcggaagcg ccagacatgc ggcccgccgg ccgaggcaaa
     4441 ctgcttttcg acttcatgcg ggccgctggt caccatcagc gtgttgacgt cctccgcact
     4501 cagaaaggga aaaatcacct caagggccgt gcctttacgt gcaaggccgc cggacatttc
     4561 aatggccgcg ggattaatgc cctccgcgcg cgtagcgcga tccaccagca ggacgccatc
     4621 cggcagatag tccatcgcgt ccacaaaccg gcctacgcgg cggcgcccct caatgactgt
     4681 gctgaaccgg atggcccaca gacccagccc tccggaaaga aagagaaaac tcatgatgag
     4741 cagatagacc ggaagcccga tgctgctgat caccgcgccg gagagactgg actcccggct
     4801 gaccgtgaaa tacccgacag ggacgccgga ataatcgtaa atggcaatgc gctggtccgg
     4861 cgcggcagct tcactgttga tcgtcatgcc ttccaggtcg ggatatgtct ggcggatctt
     4921 tcgcacatat tcgcggtcca gcgcacgcag aatcagcagc agcgcctgcc catcgtaggc
     4981 cggaatcgga gaaaatgcga gaaatgccgg tcccagcgcc gacccggcgt agccggcgct
     5041 ggtcattcgg acatcccgaa ccgcttcaga gaaactcttc cagtccagca gtgattgacc
     5101 tccgtcatcc acgcgaccgc ggctgccttc ggacaacacg acatcaagca gcgacccgtc
     5161 cggcgccaca atcgcggcgg ccagcagatt gatctgcatg atctgtcccg attcgataat
     5221 cttctggaga aacggcgact gagatgcatc aagcggaggc cgtgattcca tcgcatgctc
     5281 cagtacagcc atcatccggt cctgctcaaa agtcaaatag tcggacaagc ggttttcggc
     5341 acgctgcttg tccagtgtca gaatgcggtc ttccaggtat gaaagcgttt cataacaggc
     5401 aacggctgcc aggaccagca gcagaaggag cagtccggca taacgcattt cgcttctgga
     5461 tagcattttc atcgctgtca ccacacagaa caggctcccg gagggagcct gttccccgat
     5521 cattcgtctt ctcttccgga tgggcggaag agcccagccg cagagttccc gcgaatatga
     5581 tatcagtagg ccagaatgcg gtagaaccgc atgccgtcag ccgtcgtgtc tgtatagctc
     5641 atcacgccgc cctcgcccgg aatcgcctca gcacccggca gctccacata cgggccggcc
     5701 ggactgtcat cggaatactg gatcgagtag aaccagccct ccgcgccttc ccagtttatg
     5761 gtgatccctg aaggatcgat cgtcgtggag gccaccgccg gaggaatgtt cccagccagc
     5821 ccgatttcga cccgcaggat ccggttgctg agcgtatcgc agatatacag atatccgcgc
     5881 ggatcccact caacatcctc aggcccgtgc agcaggccgc tgctgaccag agtggcccat
     5941 tggccggaat ccgacccaac attgatttcg ctggtcatca tgtctgcggc atagaggtca
     6001 ttggagcggc caaaagtcat ccctcgagtt ttcagaagcg ttccctcaga gggcagataa
     6061 ctgccaatgc gcgctgccag tccattggtg gtgaatcgct gcacacgacc gttggaatta
     6121 tccggataat ccgccacata gaccagattg ctgcggtcca cggtcagccc aatcggggtg
     6181 cgcaccattc caacagcgta tccgttgctg gcaaaagccg tccacacgcc gcccggcgac
     6241 attctttgca cccggtgatt atccctatcc gcgacgtaaa ggttagtcag ggcatccacc
     6301 tccacatcat aaggaacctt gaactgccca agggccgtgc cggaggcggt tcctccccag
     6361 acggtccatg ttcccgtgac agaatttcgg cgctgcacgc ggttgttggc cgcatccgcc
     6421 acataaagat tgtcggcgcc gtccacggca acacccaccg gctggttgaa ctgtccggag
     6481 gatgtccccg gagaccccag attggtggtc gtgccgtctc tggtgtccag aaccataatc
     6541 cggtcattgc cggagtccgc aatataaagc ctgcgctgtg aatcaagggc caggccgcgc
     6601 ggctcgttaa gcagtccagg cacatcatcc atcagcagtt ccgttctgag atagtacccg
     6661 tccagctccg ttgtctgatt gctgaatacc gtcacaagct gttcagacgg tgacagccac
     6721 ccatccagct gcgtaaaggt gaccccgtaa tctcctgccg ccagcggaac cagggttccg
     6781 ctttcgtatt ccgtcccgtt cagtgtccac cgcgcgccaa gagctatggc ttcatcggga
     6841 tgaagctcga catagatgcc gctgacctcc tgatataatc cagggacatc aaacacaccg
     6901 ttgttggtga tcaccagcat aaccggcccc ggggtaatcc acggcggaac attcagaaac
     6961 tcaatctggt gggccccgta atccagtact ccggagatcg tgtccgaagg acgccatggg
     7021 ccattgtcca cgcgccacat ggcgccgtca gcaatgactt ccggcggttc gataatccct
     7081 cgaacatatc cttccacatc tgtgtaatgg ccgacggcag aaaccgcggc gccctcgctg
     7141 atcacaatct cccggctgac aggcgtgttc cagcccggga cgggataaaa gcggagcgtg
     7201 taggtcccaa cttcgagatt ctcgatcgtg gttccattca attgccagat catgtcatcc
     7261 cgccgggccc catttgttcc gatattcccg atgctccaga gatatccgcc gtactgggaa
     7321 tcaaaaaacc aggccgcttc attcacttcc agaggctgga tggtgacggt cagcgaaccc
     7381 ctctggacat aggatcggtt cagaaccagt gtctctccgt tggtgacggt gatcgtctcg
     7441 ttatctggcg cgctccatcc caggatgctc cgataagtga ccgtgtgcgt catgtcaaag
     7501 cgatcgagat ccgacactgt gacaccgctg tccatccacg ggccattgtc aatggaccac
     7561 tgggcgccgg ccagcacggc cgcatccggc tgcaggatga tgcgaaccga cggaggaggc
     7621 tcaatggcgc cgtttgtggt gaacgacaca cttccgttca gccgtgcggc atgcatccac
     7681 tgctgattcc agtcctttgc gaacacataa tcctcggcgc cctcctgacg gtgataatcg
     7741 ccaaaggcta ttccgttggt atcgctcgcc tgtccgtcgt cgaagcggaa gtagtgaaca
     7801 aggtcttcac cggtcgtgac ataatcataa tagtactttc tgatctccgc atccgtacgg
     7861 gcatcgcccc agattcgaac gtcatcaatg agcccggcat aatcctcgcc gatgcgcaca
     7921 aaggtggctc cgccccggcc attcaaggga ggcgcctcgt ggaaaccggt cccggacagg
     7981 gccggaacgc cgtttgtata aatggtgagc gttccattga gcgcatcata ggaggcggcg
     8041 atatgcgtcc atccaccggt ggtcgtcaca gacggtccct gcagaatgcg tttctggcct
     8101 cctgggagca catatccggc gtagatttgc agcgaagaac
//
LOCUS       DAOQWR010000123         7998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9495_length_7998_cov_4.614377, whole genome shotgun sequence.
ACCESSION   DAOQWR010000123 DAOQWR010000000
VERSION     DAOQWR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_7998_cov_4.614377"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKK36_09395"
     CDS             complement(<1..835)
                     /locus_tag="PKK36_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005027001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR94805.1"
                     /translation="MKTVYLDNNATTQVAPEVIDAMLPFFRDSWGNPSSMHTFGGNVK
                     KHIDKAREQVAALLGASPSEILFTSCGSESNNMALRGVVEASGRYPHIITSCVEHPAV
                     LEVARYLNKKGCRLTELKVNSGGHLDLEELKASLSPSSLLLSFMWANNETGVIFPIDK
                     IAPIAKEAGLIFHTDAVQVAGKLPIDMRKVPVDLLSISGHKLHAPKGIGVLYIRKGTK
                     VSPFILGGHQERSMRAGTENVPYIVGMGKACELAMEHMDEEQTVVAPMRDRLEKEILA
                     VC"
     gene            complement(832..1665)
                     /gene="nifU"
                     /locus_tag="PKK36_09400"
     CDS             complement(832..1665)
                     /gene="nifU"
                     /locus_tag="PKK36_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNR94806.1"
                     /translation="MWEYTDKVMDHFRNPRNTGVIEDADGTGEVGSMACGDALKLTFK
                     LGGDGRIADAKFQTFGCASAIAAASAMTELIKGKTIAEAEKITNQDVANFLGGLPGEK
                     MHCSVLGEQALEAAIENYRTGKPAGVKEIEGRVVCHCFGVTEKEIEHVVRENHLTTVE
                     EVTNYTKAGGGCGNCLDDIQEILDRIAGEKPKAETKGKTMTNLERIKLIEQVIATTIR
                     PALQADGGDINLVDVDGNRVTVALQGACASCPMATYTMKGFVEAQLRQAVSDELIVEA
                     Q"
     gene            1817..4600
                     /locus_tag="PKK36_09405"
     CDS             1817..4600
                     /locus_tag="PKK36_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94807.1"
                     /translation="MSKKSFLSGLLILCILLAAVLSNAQDDSPNTLYREGMGFFAEGN
                     FEMAAERFSDLISRFGNEEEFQQAMATVFYALGCSQYNIGQYEECAGTFEKFANVYPN
                     AKFVDEGLFRIGSAYFALENYEKAIEAFQNMMEKMPQSSYAEDSFYQIGVCHMLLGDF
                     KEAAESFLAFTQAFPNTALYSQATVLRARALFELDEWEQSLDALRDLEGRGRSFEHIT
                     IANFLAREIGDIAFDYTDYDLALDAYRRVQTKEFLLRNQRRLVEGIQFNLELIRRKRL
                     AGQDVEGLFRQEQRLNASLGEAQQMLANLEQMPEYDAALFHRIGRCFFNTDRFWEARV
                     AFTRVIEIAEEPMVREAALFDLALVLNRQRYFDDLIATCTRYLEEFEADEKLVQNGRV
                     PTMAYLRAEAYVNKEFFEEAEVELRDILKKYPNHNQLSRIEFYLALSLAMQEKFDEAI
                     QRFETWLKQYPDDIMVPEAEYWYPISLYYSGQHAKAMPRFREYAAKYPMSVYTPEALY
                     REAMCLYSLEQFKECAMLLGSWMERFPDHYFRWEAKVTRGGALAAIGELERAKAEYLG
                     VTKEAKAFYYMALTDCAKVFKAIATKEAYEEMAKVFMQYINDEPDSGNVIDAAYQAGW
                     ALRQTGETDKAQRFYWTILSRHGNTRGWEGFTPVFKDMMRLYGPEGQDRFRADLEQKY
                     SEALQNRRLTLGARLKMAIILSRPPEEQGAEIQKLADTFTVQHLGPEEIGFVGQYFLN
                     AGQTEKGMTYLNTLLTEFPKSAYVAVAHVRMAEAAYLAGDLEKALEYATLGVAEAFDP
                     ALYIDAQMVHSETLRALGKYEEAIPGYTEVLANRMSPKAYKPLCLLGLGDCHAAMDRP
                     EKAIAFYQRIYVLYRAYTDAVVQAYLRSGEQFEKLKDRQAAINTYTELINDEKLGAVP
                     EAAAAKERLAKLQ"
     gene            4597..5574
                     /locus_tag="PKK36_09410"
     CDS             4597..5574
                     /locus_tag="PKK36_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94808.1"
                     /translation="MNMTGRYILAAGLGLMLGGAVSAQTQPAAKSADKPRGIGPYAMT
                     VRQGEKTARTQVWLLRRARDMVWISRKTQTGSFIDMGMHQDDVVNLEIPRPSVFDAVM
                     QAANDSARRAAQRALHAVQKQFEPYRDLPGVIYDEALFLEGRLLEQLGRWDEALKLYT
                     DIISQKYESPMAASARVHAGLCLLQKKDFEGALAHLDKNTIPPDDIDLLSEVCFGRGT
                     ALQALEKYDEAIMDYLYPVVFHPFVNSNEVRCLKAVVPCYVARKDWDAVFKTFYALRK
                     DYPDAAETQETRTYLEAYTNELGRELDFQIELKDEELEEDEEEGNDDEA"
     gene            5564..6358
                     /locus_tag="PKK36_09415"
     CDS             5564..6358
                     /locus_tag="PKK36_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNR94809.1"
                     /translation="MKLNKLLTVFTVLMLAGLVLASVCFAQDEEAPAAPTKLTLMGML
                     KQGGWAMWPLGLCSFALVAFAVLNFRQISMKKMIPPDALSQIRAAASNRDLGTLWQVS
                     SGTDSFFTKGLSAGLRKINPDDPPASKGHMEEAIAETVSREESQVSFWINFLSLISAV
                     SPMIGLLGTVSGMIKAFQKIGQSGMGKPELLAANIGEALITTATGLIIAIPALFFFFL
                     FRNMLNKVIVTSEEHYSLILDDITGTGVTHLFESPSAESAQEETEA"
     gene            6386..6844
                     /locus_tag="PKK36_09420"
     CDS             6386..6844
                     /locus_tag="PKK36_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR94810.1"
                     /translation="MKLKRREQPLAEVDMTPMIDMVFLLLVFFMTAATMAKMNMTEKV
                     KLPVADKAQIPDNKRDREAITVMPNGFVTSDGSVISDAEPYMFRGKKTDAAGLSQTLT
                     ALVKNEPDLRVFMRIDRDVEYIRVQSAIRACAEAGIYDIIFGAYQTAAGD"
     gene            6846..7286
                     /locus_tag="PKK36_09425"
     CDS             6846..7286
                     /locus_tag="PKK36_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR94811.1"
                     /translation="MKIKRKKSDEKLEIQMTPMIDCVFLLLIYFMVSTTLQRQEADIG
                     VSLPGVAEQSEAVEIPDEQVIEITEAGNVLLNDLEYDEPGKSNLPELVKMLKLFKQTA
                     EANKVEAMITIAPAAKIKHQRVVDVLNACAEAKLNNVTFAVEEM"
     gene            7283..>7998
                     /locus_tag="PKK36_09430"
     CDS             7283..>7998
                     /locus_tag="PKK36_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94812.1"
                     /translation="MTNGDQHPHHKLSKGARFKLEGVVASVVVHIILLIGAASLTVLV
                     IQGRQKMMFEGKEPPSVPAKKLEHAIRVKQMEKQVRKPQILQRMVSKTPSSVALPELP
                     EMEVPDMKNMRDTIMSRSKAGSALGGLGGEGGGAGRGLTGGTGYSDAQFFGENVRTRA
                     ICILMDISASILAKGIMEDVRAEALKMLEALNPNTKFNLIVFVDGAQPFSPQMVYGTK
                     ANREAGIKWLNQEFDGRKMG"
BASE COUNT         1706 a         2178 c         2392 g         1722 t
ORIGIN      
        1 gacagaccgc gaggatttct ttttccagac ggtcgcgcat gggagccacg acggtctgtt
       61 cttcatccat atgttccatg gccagctcgc aggctttgcc catgccgacg atgtagggca
      121 cgttttcggt tcccgcccgc atgctgcgct cctggtgccc gccaagaatg aacgggctga
      181 ctttggtgcc tttgcggata tagagcacgc caattccctt gggggcatga agcttgtggc
      241 cggagatgga aagaaggtcc acgggaactt tgcgcatatc aatgggaagc ttaccggcga
      301 cttggaccgc gtcggtatgg aaaatgagtc ccgcctcttt ggcgatcggg gcgattttat
      361 cgatagggaa gatcacgccg gtttcgttgt tggcccacat gaaggaaagg agcagggaac
      421 tcggcgacag ggatgccttg agctcttcaa gatcgagatg tccgccgctg ttcaccttca
      481 gttcggtgag gcggcagcct tttttgttga gatagcgggc cacttccagg acggcgggat
      541 gttcgacgca gctggtgatg atgtggggat agcggccgga ggcctccacc acgccgcgca
      601 gcgccatgtt gttgctctcg gagccgcagc tggtgaagag aatttcggaa ggactggcgc
      661 ccagcagtgc ggcgacctgc tcacgggctt tatcaatgtg ctttttgacg tttcctccga
      721 aggtgtgcat gctggagggg ttgccccacg agtcccggaa aaacgggagc atggcgtcaa
      781 tgacttcggg cgcgacctgc gtcgtcgcat tgttgtccag atagacggtt ttcattgcgc
      841 ttcgacgatc agctcgtcgg acacggcctg acggagctgc gcttccacaa agcccttcat
      901 ggtgtaggtg gccatcgggc agctcgcgca ggctccctga agggccacgg tcacgcggtt
      961 gccgtccacg tccaccagat tgatatcacc gccgtcggcc tgcagggccg ggcggatggt
     1021 ggtggcgatg acctgttcga tgagcttgat gcgttcaagg tttgtcatgg tttttccttt
     1081 ggtttcagcc ttcggctttt cgccggcgat gcggtcaaga atttcctgaa tgtcatccag
     1141 acagttcccg caacctccac cggccttggt gtagttggtg acctcctcca ccgtagtcag
     1201 atggttttcg cggacgacat gctcgatttc cttctcggtg acgccgaagc agtggcagac
     1261 cacgcggcct tcgatttcct tgacgccggc cggctttccc gtccggtagt tctcaatggc
     1321 ggcctcaagg gcctgttcgc caagaacgga gcagtgcatt ttctcgccgg gcaggccgcc
     1381 aaggaagttg gccacatcct ggtttgtgat cttttccgcc tcggcaatag tcttgccctt
     1441 gatgagttcg gtcatggcgg aggcggcggc gatggcgctg gcgcatccga acgtctggaa
     1501 tttggcgtcc gcaatgcggc cgtccccgcc aagcttgaac gtaagcttca gcgcgtcgcc
     1561 gcaggccatg gatccgactt cccctgtgcc gtcggcatct tcaatgaccc ccgtgttccg
     1621 ggggttccgg aagtgatcca tcactttatc tgtatattcc cacattcttc atctctcctg
     1681 atgagcacca gcacaatata cgcaagtccg gcggaatttc atcaaagata tcaaaaaaag
     1741 ttgagccctg agcctttgac atcctgaata caaaagtcta gtatcgcgca aattgtttta
     1801 tactctggcg tcggttatgt cgaaaaaaag ttttctttca ggtttgctga tactctgcat
     1861 tctgcttgcg gcggtgcttt ccaatgcgca ggatgattcc cccaacaccc tttatcgcga
     1921 gggcatgggc ttttttgccg agggcaattt tgaaatggcg gccgagcggt tttccgatct
     1981 catcagccgt ttcggcaatg aggaggaatt ccagcaggcg atggcgacgg tgttctacgc
     2041 gctgggctgc tcgcagtaca acatcgggca gtatgaggaa tgcgcgggaa cctttgaaaa
     2101 atttgcgaat gtttatccca atgccaagtt tgtggacgag ggactgttcc gaatcggctc
     2161 ggcgtatttt gcgctggaaa attatgagaa ggcaatcgag gccttccaga acatgatgga
     2221 gaagatgcct cagtcctcct atgccgagga ctccttctat cagatcgggg tctgccatat
     2281 gcttctgggg gatttcaagg aggccgccga gtcttttctc gcgttcactc aggctttccc
     2341 gaatacggcg ctgtattctc aggccacggt tcttcgcgcg cgcgcactgt ttgaactgga
     2401 tgaatgggag cagtcgctgg atgccctgcg cgacctggag ggccggggac gttcttttga
     2461 gcacatcacc attgccaatt tcctcgcgcg ggaaattggc gacattgcct ttgattacac
     2521 cgactatgat ctggctctcg acgcctatcg ccgcgtccag acgaaggagt tcctgcttcg
     2581 caaccagcgc aggctggttg agggcattca gttcaatctg gaactcatca ggcggaaaag
     2641 gctggccgga caggatgttg aaggcctctt ccgtcaggag cagcgcctga atgcgtcact
     2701 gggcgaggcg cagcagatgc tggcgaatct cgaacagatg ccggaatatg atgcggctct
     2761 cttccaccgg atcgggcgct gctttttcaa caccgaccgc ttttgggagg cgcgcgtggc
     2821 gtttacgcga gtgattgaaa tcgccgagga gcccatggtg cgtgaagccg cactgttcga
     2881 tctggcactc gtgctcaacc gccagcgtta ttttgacgat cttatcgcga cctgcaccag
     2941 gtatcttgaa gagtttgaag cggatgaaaa gctggttcag aacggccgcg tgccgaccat
     3001 ggcctacctg cgggctgaag cctatgtcaa caaggagttc ttcgaggagg cggaggttga
     3061 actgcgcgac atcctgaaga agtatccgaa tcacaatcag ctttcgcgca ttgagttcta
     3121 tctggcgctt tcgctggcca tgcaggagaa gtttgacgag gccatccagc gcttcgagac
     3181 ctggctgaag cagtatccgg acgacatcat ggtgccggag gcggagtact ggtatccgat
     3241 ttcgctttac tattcaggcc agcatgccaa ggccatgccg cgcttcaggg agtatgcggc
     3301 gaaatatccg atgtcggttt atactccgga agccctgtac cgcgaggcca tgtgtcttta
     3361 ttccctcgag cagttcaagg aatgtgcgat gcttctggga agctggatgg agcgtttccc
     3421 tgaccactat ttcaggtggg aggcgaaagt cacgcgcggc ggggcccttg cggcgatcgg
     3481 cgagctggaa cgagccaagg cggaatatct cggcgtgacg aaggaggcca aggcgttcta
     3541 ctacatggcc ctgaccgact gcgccaaggt ctttaaggcg atagccacga aagaggccta
     3601 tgaggaaatg gcgaaggtct tcatgcagta catcaatgac gagccggatt ccggcaacgt
     3661 gattgatgcc gcctatcagg cgggctgggc gctgcggcag acaggcgaga ccgacaaggc
     3721 gcagcgcttc tactggacga tcctttcacg ccacggtaac acccgcggat gggaggggtt
     3781 cacgcctgtt tttaaggaca tgatgcggct ttacggcccc gagggccagg accgcttccg
     3841 ggccgatctc gaacagaaat actccgaggc gcttcagaac cgcaggctga ccctgggcgc
     3901 gcgcctgaaa atggcaatca ttctttcgcg cccgccggaa gagcaggggg cggaaatcca
     3961 gaaactcgcg gataccttta ccgttcagca tctggggcct gaggaaatcg gttttgtcgg
     4021 ccagtatttc ctgaatgccg gccagaccga gaaggggatg acctatctga acaccctttt
     4081 gaccgaattc ccgaagtctg cctatgttgc ggtggcccat gtccgcatgg ccgaagcggc
     4141 ctatctggcg ggcgacctcg aaaaggcgct ggaatatgcc accttgggcg tggcggaggc
     4201 gtttgatccg gctctctata ttgacgcgca gatggtgcat tcggagacgc tgcgggcgct
     4261 tggaaaatat gaggaggcca ttccgggtta taccgaggtg ctggccaatc gcatgtcccc
     4321 caaggcctac aagccgctct gcctgctggg gctgggggac tgccatgcag ccatggaccg
     4381 gccggaaaag gccatcgcct tctaccagcg gatctacgtg ctctatcggg cctatacgga
     4441 tgctgtggtt caagcctacc tgcggagcgg cgagcagttt gaaaagctca aggaccggca
     4501 ggcggccatt aacacttata ctgaactgat caacgatgag aaactgggcg cagtccctga
     4561 agcggctgcg gcgaaagaga gactggcgaa actgcaatga acatgacggg aagatacatt
     4621 ctggcggcgg gactgggcct gatgctcgga ggcgcggttt ccgcccagac ccagccggcc
     4681 gcgaaatccg cggacaagcc ccggggcatt ggcccttacg cgatgaccgt gagacagggt
     4741 gaaaagaccg cccgcacaca ggtgtggctc ctgcggcgcg cgcgggacat ggtctggatc
     4801 agccgcaaga cccagacagg ttcattcatc gacatgggca tgcatcagga tgatgttgta
     4861 aatctggaaa tcccgcggcc ttccgtattt gatgccgtca tgcaggccgc caatgactct
     4921 gcccgccgcg ccgcgcagcg cgcgctgcat gccgttcaga agcagttcga accgtaccgg
     4981 gatctgcccg gggtgatcta tgacgaggcg ctttttcttg aaggacggct gctggaacag
     5041 ctcggccgct gggatgaagc attgaaactg tatacggata tcatcagcca gaaatacgaa
     5101 tccccgatgg cggcctccgc cagggtccat gctggacttt gcctgctgca gaaaaaggac
     5161 ttcgaggggg cgctggcgca cctggacaaa aacaccattc cgccggacga tattgatctg
     5221 ctgtccgagg tgtgtttcgg ccgggggacg gcgctgcagg cgctggagaa gtatgacgaa
     5281 gccattatgg attatctcta tcccgtggtg tttcatcctt ttgtgaacag caacgaggtc
     5341 cgttgtctca aagccgtggt gccgtgttat gtcgcccgca aggactggga tgcggtgttc
     5401 aagacatttt atgccctgag gaaggattat ccggatgcag ccgaaacgca ggagacgagg
     5461 acgtatctgg aggcctacac gaatgagctg ggccgcgagc tggattttca gattgaattg
     5521 aaagatgaag aacttgaaga agacgaagaa gaagggaacg acgatgaagc ttaacaaact
     5581 gctgactgta ttcaccgtgt tgatgctggc cggtctcgtg ctggcttctg tctgctttgc
     5641 tcaggatgaa gaggcccccg ccgcgccgac caaactgacc ctgatgggca tgctgaagca
     5701 gggcggctgg gccatgtggc ctctgggcct gtgctctttc gctctggttg cgtttgcggt
     5761 tcttaatttc cgccagatca gcatgaaaaa gatgataccc cccgatgcgc tgagccagat
     5821 ccgcgccgcc gcgtccaatc gtgacctcgg cacgctctgg caggtgtcca gcggtacgga
     5881 cagtttcttt acaaaaggtc tttccgccgg cctccgcaag attaatcccg atgatccgcc
     5941 cgcatccaag gggcacatgg aggaagccat tgccgaaacc gtctcgcgcg aggaatcgca
     6001 ggtttcgttc tggatcaact tcctgtcgct gatttcggcg gtgtcgccta tgattggact
     6061 gctgggaacc gtcagcggca tgatcaaggc ctttcagaag atcggccaga gcggaatggg
     6121 caagccggaa ctgctggccg ccaacatcgg cgaggcgctg atcacgacgg ccaccggcct
     6181 gattattgcc atccccgcgc tgttcttctt cttccttttc cgcaatatgc tcaacaaagt
     6241 cattgtgacc tccgaggagc actattccct gatccttgat gacatcaccg gcaccggcgt
     6301 cacacatctg tttgagtcgc cctcggcgga atctgcgcag gaagaaacag aagcttaatt
     6361 caaaagcagc aaacccgaaa acggcatgaa actgaaaaga agagaacagc ccctggccga
     6421 agtggacatg acgcccatga tcgacatggt cttcctcctg ctggtgttct tcatgaccgc
     6481 cgcgacgatg gcgaagatga acatgaccga aaaggtcaag ctgcctgtgg ctgacaaggc
     6541 gcagattccc gacaacaaga gggatcgcga ggccatcacg gtcatgccga acgggtttgt
     6601 gacttccgac ggctcggtga tcagcgacgc ggagccctat atgttccgcg gaaagaaaac
     6661 ggacgccgcg ggactctcgc aaacgctcac agctctggtt aagaacgagc ctgatttgcg
     6721 ggttttcatg cggattgacc gcgacgtcga atatatccgg gttcaatcag ccattcgggc
     6781 ctgcgcggaa gccggcattt acgacatcat cttcggagcc taccagacgg cggcgggaga
     6841 ctgagatgaa gattaagcgc aaaaaatccg acgagaaact ggaaattcag atgacgccca
     6901 tgattgactg cgtcttcctg ctgctcattt actttatggt cagcacgaca ctccagcgcc
     6961 aggaggcaga tatcggcgtc tctcttcccg gggtagccga gcagtcggag gctgtagaaa
     7021 tcccggatga acaggtcatt gagatcacgg aggccggaaa cgtgcttctg aacgaccttg
     7081 aatacgatga gcctgggaaa tcgaacctgc ccgaactggt gaagatgctg aagctcttca
     7141 agcagacggc cgaagccaat aaagttgagg ccatgatcac cattgcgccc gcggccaaaa
     7201 tcaagcatca gcgggtggtg gatgtgctca atgcctgcgc cgaggccaaa ctgaacaatg
     7261 taacctttgc cgtggaggaa atgtgacaaa cggcgatcaa catcctcatc acaagctctc
     7321 aaaaggcgcg cgtttcaagc tcgagggcgt cgtcgccagc gtggtggtgc atattattct
     7381 gctgatcggt gccgcctcgc tgaccgtcct tgttattcag ggccggcaga aaatgatgtt
     7441 cgagggcaag gagccgccct ccgttcccgc aaagaagctg gaacacgcca ttcgcgtgaa
     7501 gcagatggaa aaacaggtcc gcaagccgca gatacttcag cggatggtgt ccaagacccc
     7561 gtcttctgtg gcgctcccgg aacttccgga aatggaagtt cctgatatga agaacatgcg
     7621 cgataccatc atgtcgcgct ccaaggccgg gtccgcgctt ggcgggcttg gcggcgaggg
     7681 cggcggcgcc ggtcgcggcc tcaccggggg caccggttat tccgacgcgc agttttttgg
     7741 cgaaaacgtc cgcacacgcg ccatctgcat cctcatggat atcagcgcca gtattctggc
     7801 caagggaatc atggaggatg ttcgtgccga agcgctcaaa atgcttgagg cgctgaatcc
     7861 gaacacgaag tttaacctga tagtctttgt ggacggcgcg cagccgttct cacctcaaat
     7921 ggtctatggc accaaagcca accgtgaagc gggaataaaa tggctcaatc aggaatttga
     7981 cggccgcaaa atgggcaa
//
LOCUS       DAOQWR010000124         7937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9592_length_7937_cov_5.147932, whole genome shotgun sequence.
ACCESSION   DAOQWR010000124 DAOQWR010000000
VERSION     DAOQWR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9592_length_7937_cov_5.147932"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PKK36_09435"
     CDS             complement(<1..1106)
                     /locus_tag="PKK36_09435"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNR94813.1"
                     /translation="MNALNTERIRRDFPILARTMRGGKRLVYLDNAATTQKPECVIEA
                     VCDYYRNHNANVHRGFHQLSEEATEILESARARVADFIGAGSPQEVVFTRGATESINL
                     AASAYGAAALRPGDQILLTVMEHHSNLLPWQQLARQTGAELCFIPGREDGALDMDAFQ
                     QMLSPKVKILAMTHMSNVLGTINPVRQCIAAAHSAGARVLIDAAQSLPHFKVDVADLD
                     CDFLAFSGHKMLGPMGIGVLYAKRELLDRMRPYQTGGSMIHRVALRDAVWADVPYKFE
                     AGTPNVAGAAGLKAAIDYLEAAGMDRIFQHEQELVRYALQRLDALSGIQIVGRAAHRG
                     AVIAWHMKNVHPHDMAQFADQEGIAIRAGHHCAQP"
     gene            complement(1103..1804)
                     /locus_tag="PKK36_09440"
     CDS             complement(1103..1804)
                     /locus_tag="PKK36_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94814.1"
                     /translation="MNDRPLLQADAIDKSFVMGAVVIPVLKGVSLSLGRGERTAIMGA
                     SGAGKTTLLHVLGGLESPTAGRVMFDGTDVYSQSPARRTALRASQTGFIFQAYYLLPE
                     LTVLENVMLPAMALPRRLQPKSRATRDRAESLLSSVGLSGRLHHLPTELSGGEQQRTA
                     VARAMMNNPEILFCDEPTGNLDSKTGDTVLQCLFRLVEEHQVTLLLVSHNRALCDQCD
                     RVLFLRDGRLETDQE"
     gene            complement(1797..3023)
                     /locus_tag="PKK36_09445"
     CDS             complement(1797..3023)
                     /locus_tag="PKK36_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR94815.1"
                     /translation="MKLPFSLFLALKYLRPKRTFVSVVTLISVLGVLLGVAVLVIVLS
                     VMSGFDDMWRDKILGFNAHLTVTGWGIMEDEDAVMERLAEVPGVKGTAPFVQGLVFIQ
                     HEDHVYTPVMRGIDPEMERRISQIPAHMAEGVFSVEDDEVILGRDLAARVGAGLGDRI
                     LVYSPKNFTVADELRLPEELTVSGIFELGMWDFDMGFMLTSLDTARSMYSMERGVHGI
                     QVMTGDPFRAPSIAARMQVDLGPEYEVQTWMDMNRQLFSALKVEKNMMFFLLIIISVV
                     AAFGITNTLITLAVQKTKEVGLLKALGFSSGGIMRVFLWQGWICGVLGTLSGMAAGLL
                     VLYYRNDLMSWMNRRLGMELLPKDLYHLSEIPSTTSLTDVAMVALVVVIICTLAGLVP
                     AWRAARLDPARALHYE"
     gene            complement(3023..4468)
                     /gene="lysS"
                     /locus_tag="PKK36_09450"
     CDS             complement(3023..4468)
                     /gene="lysS"
                     /locus_tag="PKK36_09450"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNR94816.1"
                     /translation="MENSENSSEVRAQRLENMEKLKAMGYQPFGKAYSRTGRLSDIAA
                     DFEEHKPVRAAGRLLARRDMGKSTFAHISDGSSRLQIYVKKDEVGEDAYAAFKILDIG
                     DQLGVEGELFVTRTGEKTIKVQKWELLSKALLPLPEKWHGLQDTEIRYRQRYLDLIAN
                     PEVREVFNKRVAMVREIRTFLAERGFSEVETPMLQVMAGGAAAKPFVTHYNALKTDMY
                     LRIAPELYLKRLLVGGFDKVFEMNRNFRNEGLSRTHNPEFTMLEIYEAFGDVNSMKSL
                     VQDLVTHVAQKVFGTLVVGEGDRRIDLTPPWRTAAYRDLVKERAGADWFDLPVDEARK
                     RAEQLGCELDPAWGMAEVTHEVYEKVIEKTLMNPTFVTRLPTELVPLAKTCADDPSVV
                     DVFELEIGGCEIAPGYTELNDPLEQRRRLAEQAGEDVQKMDQDFLTALEHGMPPAGGM
                     GIGIDRLMLILSGCETIRDVILFPHLKPRNA"
     gene            4602..4687
                     /locus_tag="PKK36_09455"
     tRNA            4602..4687
                     /locus_tag="PKK36_09455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4635..4637,aa:Leu,seq:caa)
     gene            4737..5684
                     /locus_tag="PKK36_09460"
     CDS             4737..5684
                     /locus_tag="PKK36_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94817.1"
                     /translation="MAAALLMLPAVLAAKPYEKGPEGPTWRWWRRPEKSAPAAQMIHA
                     RDLLQAGKLRKAAKEFRALVDTWPDVPEAADAQREYARVLHRRGKSDDAFAEYQFLLE
                     RYGNQAPYNDTLEQMLTIATERMNRKAATLLFGGFTDPLKAVPLFEKILELAPGWEKA
                     PEVSWLIGRANELGGEREEAVKCYAATQYRYPDSPFAEKSAFDKSYVLYTMSQQRLKD
                     DYLAESAWTSLTLYLQSWPHSERADKAIQYRNHLFSQRAQRAYEKASFYHKTAKNPEA
                     ALIAYRRFLQQFPNSGWTTLAEMRIQELTNLLERKNANK"
     gene            5671..6186
                     /gene="lptE"
                     /locus_tag="PKK36_09465"
     CDS             5671..6186
                     /gene="lptE"
                     /locus_tag="PKK36_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNR94818.1"
                     /translation="MQINRMCAGLFCAVLLLNLCGCVGYQLGSMLPGDIRTVCVPTFV
                     NNTDEPNIEIETTQATIREIQQDGSLKIASCEEADAILEVVLKEYELEPISYGRDRET
                     TAEEYRIWIRASYVFRKLSDDTVVSEAPNVRGQSTTEFSGDLSLMKRTELPRTARDLA
                     HEIVERMVEAW"
     gene            complement(6218..7168)
                     /locus_tag="PKK36_09470"
     CDS             complement(6218..7168)
                     /locus_tag="PKK36_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNR94819.1"
                     /translation="MKNYSELLPRFLDHYDLMVTGYEHVRTSENVVFRLQTDNGRYYA
                     LRIRRIIGDYQEQIASELMVLHDFHTRTGADIPAPLAARSGELFCMVNDGGHDYMCII
                     FNWVSGVHLGGNEITSAHMASMARSVAQLHGFSSAYRPPAGFVRPVYDDGWFFGEKSW
                     TAGDEFMKQIEPQACSYLRDVNDLIRARLKEYPRTAQTFGLIHYDLHAGNFLFDDGKA
                     NMIDFDECGFGWYLFDLAHILFEFIGDPRFDAFKEVVAKTYGRGRCSDSDLNLFLALQ
                     GIAYMNWLYRLFWRDGTSDAQEYWVPVIVGRLKTVLRSMR"
     gene            complement(7165..7704)
                     /locus_tag="PKK36_09475"
     CDS             complement(7165..7704)
                     /locus_tag="PKK36_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNR94820.1"
                     /translation="MPNTEFISDAEIYRRVIQTDVPQAREFLWLATADLKDLHVAQGR
                     RMIPFLGVLSALCAQGVAIRLLHAKEPGPNFRRDFDRYPNLISGMERILCPRVHFKSV
                     IVDGRVAYTGSANLTGAGMGAKSPQRRNFESGIVTTDASMVRQIMEQFDQVWMGVHCG
                     KCGRRPHCPDPITGTQKRK"
BASE COUNT         1645 a         2436 c         2269 g         1587 t
ORIGIN      
        1 ggctgagcgc agtggtgacc cgcgcgtatg gcaatgccct cctggtccgc gaactgggcc
       61 atgtcgtgcg gatgcacgtt tttcatgtgc catgcgatca cggcgccccg gtgggcggct
      121 cggcccacga tctggatccc ggagagggcg tccagacgct gcagggcgta ccggacgagt
      181 tcctgttcat gctggaagat gcggtccatc cccgcggctt cgagatagtc aatggccgcc
      241 ttcagccccg ccgcgccggc cacgttgggc gtgccggcct cgaacttgta cggaacatcg
      301 gcccagaccg catcccgaag ggctacgcga tggatcatgc tgccgcctgt ttgatacgga
      361 cgcatccggt ccagcagttc acgcttcgcg tacagcacgc caatgcccat ggggcccagc
      421 attttgtggc cggagaaggc aaggaaatcg cagtccagat cggccacatc caccttgaaa
      481 tgcggaaggc tctgcgcggc atctatgagc acccgcgccc cggctgaatg cgcggcggca
      541 atgcactgcc tgaccggatt gatggttccc agcacgttgg acatgtgggt catcgccagt
      601 atcttcactt tcggagacag catctgctga aaggcgtcca tgtcgagcgc gccgtcctcc
      661 cggccgggga taaaacagag ttccgcgccg gtctgccggg cgagctgctg ccagggaagc
      721 agattgctgt ggtgctccat cactgtcagg agaatctgat ctcccggccg gagcgccgcc
      781 gcgccgtaag ctgacgccgc cagattaatg ctttccgtgg cgccacgcgt gaacaccacc
      841 tcctgcggag aaccggcgcc gatgaaatcc gccacccgcg cgcgggcgga ttcaaggatt
      901 tcggtcgcct cctcggacag ctgatggaat ccgcggtgca cgttggcgtt gtggttccga
      961 taatagtcgc acacggcctc gatcacgcat tccggcttct gcgtggtggc cgcgttgtcg
     1021 agataaacca ggcgcttccc cccgcgcatt gtgcgggcaa gaatcgggaa atccctccgg
     1081 atccgctctg tgttcagcgc attcattcct gatcggtttc cagccgtccg tcgcggagaa
     1141 acaggacgcg gtcgcactgg tcgcaaagcg cgcggttgtg cgaaaccagc aggagcgtga
     1201 cctgatgctc ctccaccagc cggaagaggc attgaagcac agtgtccccc gtctttgaat
     1261 ccaggttacc ggtgggttca tcgcagaaaa gaatttccgg gttgttcatc atcgcgcgcg
     1321 ccaccgcggt gcgctgctgc tctccgccgg atagttccgt cgggagatga tgcaggcgtc
     1381 cggaaagtcc cacgctggag agcaggcttt ctgcgcggtc tcgcgttgca cggcttttcg
     1441 gctgcagtct tcgcgggagg gccatcgccg ggagcatgac attttccagc acggtcagct
     1501 cgggcagaag ataataggcc tggaaaataa agccggtctg gcttgcgcgc agcgccgtcc
     1561 gccgggccgg ggactgactg tacacatccg tcccgtcaaa catcacgcgg ccggcggttg
     1621 gagattccag gcctccgagc acatgcagca gtgtggtttt cccggcgccg cttgcgccca
     1681 tgatggccgt gcgctcgccg cgaccgagcg acagtgaaac ccccttcagg acagggatca
     1741 ccacggcgcc catgacgaag ctcttgtcaa tggcatccgc ctgaagcagt ggacggtcat
     1801 tcatagtgca gggccctcgc aggatccaga cgggcggcgc gccacgccgg caccagcccc
     1861 gccagcgtgc agatgatcac caccaccagg gccaccatcg ccacatccgt cagcgatgtc
     1921 gtggacggga tttcgctcag atgatagaga tccttcggaa gcagttccat gcccagccgc
     1981 cggttcatcc agctcatcaa atcgttccgg taataaagaa caagcagtcc ggcggccatg
     2041 ccggacagcg ttccgagcac gccgcagatc catccctgcc agaggaaaac ccgcatgatg
     2101 ccgccggagc tgaagccgag agccttgagc aggccgacct cctttgtctt ctgcacagcc
     2161 agcgtgatca gggtgttggt gatgccgaat gccgcgacca cggatatgat gatgagcagg
     2221 aagaacatca tgttcttttc caccttcagc gccgaaaaga gctgccggtt catgtccatc
     2281 caggtctgaa cctcatattc gggaccgaga tccacctgca ttcgggcggc aatggatggc
     2341 gccctgaagg gatccccggt catcacctga atgccgtgca ccccgcgctc catgctgtac
     2401 atgcttcgcg ccgtgtccag cgaagtgagc atgaatccca tgtcaaaatc ccacatgccc
     2461 agctcaaata tcccgctcac cgtcagctcc tccgggagcc gcagttcgtc ggccacagtg
     2521 aaatttttcg gtgagtaaac cagaatccgg tcgccaaggc ccgcgccgac gcgcgccgcg
     2581 agatcgcggc cgagaatcac ttcgtcatct tcgaccgaaa agacgccttc cgccatgtgg
     2641 gcggggatct gactgatgcg cctctccatc tcggggtcaa tgccgcgcat gacaggagtg
     2701 taaacgtgat cctcgtgctg gataaacacg agcccctgca caaagggcgc ggtgcccttt
     2761 actccgggaa cctccgcaag ccgctccatg acagcatcct cgtcctccat gatcccccag
     2821 cccgtcacgg tgaggtgcgc gttgaatcca agaatcttgt cgcgccacat gtcgtcgaac
     2881 ccgctcatca cggacaggac aatgaccagc accgccacgc ccagcagcac ccccagcacg
     2941 gaaatcagcg tcacaacgga aacaaacgtg cgcttggggc ggagatactt gagagccagg
     3001 aacagcgaaa acggaagctt catcaggcat tcctgggttt cagatgggga aacagaatca
     3061 catcgcggat ggtttcgcat cccgacagaa tcagcatcag acggtcaatg ccaatgccca
     3121 tgcctccggc gggcggcata ccgtgctcca gagcggtcag gaaatcctgg tccatcttct
     3181 gcacatcttc gccggcctgt tccgcaaggc ggcggcgctg ctccagcggg tcattcaact
     3241 cggtgtagcc gggggcgatt tcgcacccgc cgatttccag ctcaaacaca tccaccaccg
     3301 aaggatcatc cgcgcaggtt ttcgccagcg ggaccagttc cgtgggcagg cgggtgacaa
     3361 acgtcgggtt catcagggtt ttttctataa ccttctcata cacttcatgg gtcacttcag
     3421 ccatgcccca cgccgggtcc agttcgcatc cgagctgctc cgcgcgtttg cgggcttcat
     3481 ccaccggcaa atcaaaccag tcggcgcccg cgcgctcctt caccagatcc ctgtacgcgg
     3541 ccgtgcgcca gggcggcgtc aggtcaatcc gccggtcccc ttctcccaca accagcgtcc
     3601 cgaacacctt ctgcgccaca tgcgtcacga ggtcctgcac cagagacttc atgctgttca
     3661 catcgccgaa ggcctcgtag atctccagca tggtgaattc ggggttatgc gtccgcgaaa
     3721 gcccttcgtt gcggaaattg cggttcatct cgaaaacctt gtcgaacccg cccaccagca
     3781 gccgcttcaa atagagttcg ggcgcaatgc gcagatacat gtctgtcttc agggcgttgt
     3841 agtgggtcac gaagggcttg gcggcggcgc cgccggccat gacctgcagc atcggtgttt
     3901 ccacttcgga aaaaccgcgc tccgccagga atgttctgat ctcgcgcacc atggccacgc
     3961 gcttgttgaa cacctcgcgc acttcgggat tggcaatcag gtcgaggtag cgctggcggt
     4021 accggatttc cgtatcctgc aggccgtgcc atttttcagg cagcggcagc agggccttgg
     4081 aaagaagctc ccatttctgg accttgatgg tcttttcgcc ggtgcgggtg acaaacagtt
     4141 cgccttcaac gccaagctga tcaccaatgt caagaatctt gaaggccgca taggcgtcct
     4201 cgccgacctc atcctttttc acataaatct gcagccggct gcttccgtcg ctgatatgcg
     4261 cgaaagtgga cttgcccatg tcgcggcgcg ccaggaggcg gccggccgcg cgcaccggtt
     4321 tatgttcttc aaaatccgcg gcgatatccg acagccggcc ggtgcgggaa taggctttcc
     4381 caaagggctg atatcccatc gccttaagct tttccatgtt ctcgaggcgc tgcgcccgca
     4441 cttcgctgct gttttcactg ttttccatga tgattgctcc aaaaagactg cggagagact
     4501 agcccccggc cccggtgaag tcaagaaacg cccattcgca acatcagcgt ccagataatg
     4561 attgcgatgc cgggatgaat gttctataga tgccgcttca cgccggagtg gcgaaatggc
     4621 agacgcactg gactcaaaat ccagcgcctt cacgggcgtg tgggttcgac tcccacctcc
     4681 ggcaccatga tgcagaacag gtgagaacaa tgcaatggac gggtaaaagt ttgagaatgg
     4741 cggcggcttt gctgatgctg cccgccgtgc tcgccgccaa gccctatgaa aagggacctg
     4801 aaggccccac ctggcgctgg tggcgaaggc cggagaaatc cgcgccggcg gcgcagatga
     4861 tccatgcccg ggacctgctg caggccggga agctgcgcaa ggctgcaaag gagttccgcg
     4921 cgctggtgga cacctggccg gatgtccccg aagcggccga tgcgcagcgc gaatatgccc
     4981 gggtcctgca ccggcgcgga aaatctgatg acgcttttgc tgaataccag ttcctgctcg
     5041 agcgctacgg caatcaggct ccgtacaatg acacgcttga gcagatgctg accattgcca
     5101 ccgagcggat gaaccgcaaa gccgcgacgc tccttttcgg cggattcact gatccgctca
     5161 aggccgttcc tctctttgag aaaattcttg agctggcccc gggatgggaa aaggccccgg
     5221 aagtgtcctg gctgattgga cgcgccaacg aactgggcgg cgaacgggag gaggcggtga
     5281 aatgctatgc cgccacacag taccgctatc ccgattcgcc ctttgcggaa aagtcggcct
     5341 ttgacaaatc ctatgtgctt tacaccatgt ctcagcagcg gctcaaagat gattatctcg
     5401 cggagtccgc ctggaccagc ctgacgctct atctgcagtc atggccgcat tccgagcgcg
     5461 cggataaagc cattcagtac cgcaatcatc ttttcagcca gcgcgcacag cgggcttatg
     5521 aaaaggccag cttttaccac aagacggcca aaaacccgga agccgcgctg atcgcctacc
     5581 gcagatttct gcagcagttt ccgaattcag gatggacgac gctggcggaa atgcgtatac
     5641 aggagctcac aaaccttttg gagcggaaaa atgcaaataa atagaatgtg tgcgggtctt
     5701 ttttgcgcag tgctgctgtt gaatctctgc ggctgcgtgg gctatcagct tggatccatg
     5761 cttccaggtg atattcgcac cgtctgcgtg cccacctttg tgaacaacac ggatgagccg
     5821 aacattgaga ttgagacaac ccaggcgacc attcgcgaga ttcagcagga cggatccctg
     5881 aaaatcgcat catgcgagga ggctgacgcc attctcgaag ttgtgctcaa ggaatacgaa
     5941 ctggagccca tcagctacgg ccgggaccgc gaaaccacgg ccgaggagta ccgcatctgg
     6001 atccgggcca gttatgtgtt ccggaaactt tccgacgaca ccgtggtttc cgaggccccc
     6061 aacgtccggg gccagtctac caccgaattt tcaggggacc tttctctgat gaaaaggacc
     6121 gagttgcccc gcaccgcacg cgacctcgcc cacgagatcg tggagcgcat ggtcgaagca
     6181 tggtaggaat cctccggcag gacggcggca tacgcattca gcgcatggac cgcaggaccg
     6241 tcttcaggcg accgacaata accggaaccc agtattcctg agcatcagag gttccgtcgc
     6301 gccagaacag gcggtacagc cagttcatat aggcgatgcc ctgcagagcc agaaaaagat
     6361 tcaggtcgct gtctgaacaa cggccgcgtc cataggtttt tgccacgact tccttgaaag
     6421 cgtcgaaacg cggatccccg atgaattcaa aaagaatgtg ggccagatcg aagagatacc
     6481 agccgaagcc gcactcatcg aaatcaatca tgttcgcttt accgtcatcg aacaggaaat
     6541 tgccggcatg caggtcataa tgaatgagac caaaggtttg cgcggtgcgc ggatattcct
     6601 tcaggcgcgc ccggatcaag tcattgacgt cccgcagata tgagcaggcc tgcggttcaa
     6661 tctgcttcat gaattcatcg cccgcggtcc acgatttttc gccaaagaac cagccgtcgt
     6721 cataaacggg ccgcacaaat ccggcgggcg gccggtaggc tgaagagaag ccgtgcagct
     6781 gcgccacgga tcgggccatg gatgccatgt gggccgaagt gatttcattt cccccgagat
     6841 gcactccgct cacccagttg aagatgatgc acatgtaatc atgtccaccg tcgttaacca
     6901 tgcagaaaag ctctccgctc cgggcagcca gcggcgcggg tatatcagcg cccgtgcgcg
     6961 tatggaagtc gtggaggacc atcagttccg aggcaatctg ctcctgatag tccccgatga
     7021 tcctgcggat gcgcagcgca taataacggc cgttgtctgt ttgcagtctg aacaccacgt
     7081 tttccgaagt gcgcacatgc tcgtatccgg tcaccatgag atcgtagtga tcaagaaagc
     7141 gcggcagcag ttcggaataa tttttcactt gcgcttttga gttcccgtta ttggatcagg
     7201 gcagtgcggc ctgcggccgc atttcccgca gtgcaccccc atccacacct ggtcgaactg
     7261 ctccatgatc tgccggacca tggatgcatc cgttgtcaca atcccggatt caaaattgcg
     7321 gcgctgaggg ctttttgcgc ccatgccggc tccggtcagg tttgcacttc cggtataggc
     7381 cacgcggccg tccacaatga cggacttgaa gtgcacccgc gggcacagga tgcgctccat
     7441 gccggaaatc agattgggat accggtcaaa atcgcgccgg aagttcgggc ccggctcctt
     7501 ggcgtgaagc aggcggatcg caacgccctg cgcgcagagc gcggacaaca ccccgaggaa
     7561 cgggatcatg cgccggccct gcgccacatg gaggtccttc aggtccgccg tcgcgagcca
     7621 caggaattcc ctcgcctgcg gcacgtcggt ctgtatcaca cgccggtaaa tctccgcatc
     7681 gctgatgaat tcggtgtttg gcatttctaa atggatttgg cgcctgtcac agggcgtttt
     7741 gtcagaaaac acgatttttc accttttggg ggcattttcg ggcatttttt gacgttttta
     7801 ggccgttttt gaccattttt tggcctttta gggccatttt tcatcatttt tgaggccttt
     7861 tcggcctgcc attaccggat gagataaatc atcttgtctt cgacgccatc gcgctcaaag
     7921 tctatgacaa cggtatt
//
LOCUS       DAOQWR010000125         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9834_length_7740_cov_5.656344, whole genome shotgun sequence.
ACCESSION   DAOQWR010000125 DAOQWR010000000
VERSION     DAOQWR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9834_length_7740_cov_5.656344"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2383
                     /locus_tag="PKK36_09480"
     CDS             104..2383
                     /locus_tag="PKK36_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94821.1"
                     /translation="MRTAILLSAFCMLAASVCAQADRDRQFADGLFARGYHEMAAQEY
                     QELLAQDAARGDADVILYRLAECLRKSGNLTGAAETCGKLAAEHPRSSYAARARLRRA
                     EILVEQGGSDEALVLLNALIDSSPEAEISAGALYHQGMILKETGQDAKAEQAFSRIVA
                     QHRKSPYASYAALELAGIRRADKPSAAEELYKQVAAENSSPRLAAEAVFQQGVMAFDR
                     KKYRESAAAFNRLLKDWPDDLRAAETPLRAAWALFYAEDYSGCSALAGRHGEESSDAW
                     LYLKAACARKLGREDEAERLYEILIASHPGSPWHAAAVYEQARIAVGRKKYKQAADML
                     KPVMEDSSVGEAAHLLMIEALLSDGDADQALKTCRAFAERFPASRELPAVLLRQAEIL
                     IQSEIFPEASEVLTRIVQNYPKSDAACAAQYNLAFCAEKENRWEDALAGWRAARERCG
                     DSALVEQASYREALVAMQLGRASAASAFEKFIRDYPKSENVHEAAWRLGLLLTRAGKT
                     EEAEKVYRTSLSRNPPEEWRDRLRIPLAALLQQRGQEDEPVALLQPLPAKAAMDDMPP
                     ALMHWLAGRLLAKNAFAEAETIALALAKRKDAAGWTQDGWHLAGRAAVALKKTARARE
                     AFKTAAEMTGGDTRTSADAALQWGLLAIEDSATDEAFGAFTLAAERSPGDDLLEIRAR
                     SYYGLGRVAEVKDDCDAASRYYLSVGLLFDEPSLVPECLWRAAECLGKLQRNDEKNLT
                     LKELRERYPDSEWAKKELP"
     gene            2384..2980
                     /locus_tag="PKK36_09485"
     CDS             2384..2980
                     /locus_tag="PKK36_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94822.1"
                     /translation="MKEQDWDIKARSEACSKCEHEYEDQEPFYSKLTFEEDGYHRSDF
                     CEKCWTDEVQASALSRWRTIYRSPAPPPEPAVKKETAESLLRELIETDRMEQANTIYI
                     LAVMLERKRILVERDVQEREDGTLIRVYEHKKTNEVFMIPDPRLKLAELETVQAEVIA
                     MLDGTARAAQQELSAPVGGEPAPEAAPENSPPEAPPAS"
     gene            2984..4549
                     /locus_tag="PKK36_09490"
     CDS             2984..4549
                     /locus_tag="PKK36_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNR94823.1"
                     /translation="MLDVKITGGLVFDGTGASGRSADIGITGDAITEIGDLSSAPAGK
                     IIDARGKCVCPGFIDVHSHSDAYLLIQPDAMSKIFQGVTTEIVGQCGASASPLQGEYK
                     MPSDWRDKKYPKEWHTASEYRAALQSVVPAVNVGFLTGHSAVRAGVMGYEPRQASAAE
                     LAAMRQLLEKSLNEGSRGFSTGLVYPPGSCADPSEIWALAGIVAKHNGIYASHMRSES
                     AGLIGAVAETIEVCRRTGVSTQISHLKTAGPANWPKIDRVLEIIRSARAEGLPVMSDR
                     YPYTASCTDLDIVLPAWASTGGPDAVLGRIRDSATRAKILAELKKSRADADWERIMIG
                     SVSHPDHANFPGLRLPEISEQMKVHPAEALLKIIEKDELKTGGIFFGMSEDNMWRILS
                     EPYVMIGSDASIRAPAGPLSKDHPHPRAYGAFPRFIRKVIDEKRMPLEEAVRKMTSLP
                     AEHFKLKGRGRLAKGFNADITVFDPEKIADKATFAKPHQLAAGMSAVLVGGCVTLLDG
                     KLSGQRSGSVLAG"
     gene            complement(4519..4923)
                     /locus_tag="PKK36_09495"
     CDS             complement(4519..4923)
                     /locus_tag="PKK36_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNR94824.1"
                     /translation="MKNRSCIFCEMDSARVIAREGSCYAVYDGFPVSEGHILIIPERH
                     IASFRELTEDEWLSVHKLAKKLSARLLEQDKTIDGFNLGINDGKAAGQTIFHAHVHLI
                     PRRKNDVENPRGGVRHVIPCKGCYPANTEPER"
     gene            complement(4938..5013)
                     /locus_tag="PKK36_09500"
     tRNA            complement(4938..5013)
                     /locus_tag="PKK36_09500"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4978..4980),aa:Phe,seq:gaa)
     gene            5252..5392
                     /locus_tag="PKK36_09505"
     CDS             5252..5392
                     /locus_tag="PKK36_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94825.1"
                     /translation="MAKPNYQFEKRSRELAKKKKKEEKRLRKQAEKDARSTADAPVSS
                     SQ"
     gene            5603..5935
                     /locus_tag="PKK36_09510"
     CDS             5603..5935
                     /locus_tag="PKK36_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR94826.1"
                     /translation="MGTKLYVGNLSFETTEDDIRDLFSSCGTVTSCNLIIDKFTSKSR
                     GFAFVEMASQAEATKAISEMNGKEVSGRALTVNEARPREERSGGGGGGYRGGNDRRGG
                     GGGRRERY"
     gene            6122..6835
                     /locus_tag="PKK36_09515"
     CDS             6122..6835
                     /locus_tag="PKK36_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94827.1"
                     /translation="MADISVSCPSCNHVCKISEYVSAETIPCPACGAPVQIPTTSLPS
                     EASIQSTLRLKKNFKEGLGEKKAAPPPPRKEIKRSSSMEKIHQVKEKPSGPRAIWGVL
                     TLLVLGGGMFYLLYSLGGNPDYLKYYLWIRNGTGLLALGLVYLSAFRESHIQGLLTLL
                     IPPYALYYVYARADSYVLRAMFTALIIALGAEFKYMRSDSLLIRFNDSVAAFIQSGED
                     LIDAANKEVETTDASKKFR"
     gene            complement(6871..7473)
                     /locus_tag="PKK36_09520"
     CDS             complement(6871..7473)
                     /locus_tag="PKK36_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR94828.1"
                     /translation="MSFFDRLFGKRSGNAAPAAKILLADVMSIGGGSNRLPPKVQIDI
                     LHSLSRYAEKEKITVHAMLTGEPLRSVPENESFRNVQVSYAASVQELPALAAKLAKRY
                     GRKGVVAVTSNRSVEDDVRQAGAALLRASTFKKALSGNGGQGDSDSRNPSQDRHRRNR
                     NRPRRERQQAPAAEPEQKPAEKAPEKKPDDGGISDLIDLV"
BASE COUNT         1635 a         2156 c         2322 g         1627 t
ORIGIN      
        1 gaccagaaga cgccctatca gcacgtgatc cgggtgcttg atctctgccg gcaggctgat
       61 atctggaata tctcgtttgc gaccagtgtg ccggaggaat agcatgagga ctgcgattct
      121 tctatctgcc ttctgcatgc ttgcggcgtc ggtctgcgcg caggcggacc gtgaccggca
      181 gttcgccgac ggactcttcg cccgcggcta tcacgaaatg gccgcccaag aatatcagga
      241 acttcttgcg caagatgccg ctcgcggaga cgctgatgtc atcctgtacc gtctggccga
      301 atgcctgcgc aaaagtggaa atctgacggg ggccgctgaa acctgcggta agctcgcggc
      361 tgagcatccg cggagttcct atgcggcccg cgcccgcctg cgccgggctg aaatactggt
      421 ggagcagggg ggcagcgacg aagcccttgt cctgttgaac gcactgattg attcctctcc
      481 cgaagcggag atttccgccg gcgcgctgta tcatcaggga atgatcctga aggaaacggg
      541 gcaggatgca aaggcagagc aggccttctc cagaattgtg gctcagcaca ggaagtcccc
      601 gtacgcatcc tatgccgcgc tggaactggc gggcattcgc agggcagaca agccgtccgc
      661 cgccgaggag ctttataagc aggtggcggc tgaaaactct tctccccgtc tggccgctga
      721 agcggtcttc cagcagggag tgatggcgtt tgaccgcaag aagtatcgcg agagtgccgc
      781 cgcgttcaac cgcctcctga aggattggcc ggatgatctg cgtgccgcgg aaacaccgct
      841 gcgtgcagca tgggctttat tctacgcgga ggattattcc gggtgctcgg cgctggccgg
      901 caggcacgga gaggaatctt ccgatgcgtg gctctatctg aaagccgcct gcgcccgcaa
      961 actgggccgc gaggatgagg ccgaacgtct ttatgaaatc ctgattgcca gtcatccggg
     1021 aagcccctgg catgccgccg ccgtgtatga acaggcccgg attgccgttg gccggaagaa
     1081 atataaacag gcggctgaca tgcttaagcc cgtgatggaa gactcgtccg ttggcgaggc
     1141 cgcccatctg ctgatgatcg aagcgcttct ttccgacgga gatgccgacc aggctttaaa
     1201 aacatgccgg gcctttgctg aacggtttcc cgcaagcagg gagcttccgg ctgtgctgct
     1261 tcgtcaggcc gaaattctga ttcagagcga aatctttcca gaggcgtcag aagtgctgac
     1321 ccgtatcgtg cagaattatc cgaagtcgga tgcggcctgc gcagcgcagt acaatctggc
     1381 gttttgcgcg gagaaggaga atcgatggga ggatgctctt gcgggctggc gggctgcgcg
     1441 tgaacgctgt ggagactccg cccttgtcga acaggcatct taccgcgagg ctcttgtggc
     1501 catgcagctc ggccgggcct ccgcagcctc cgcgtttgaa aaattcatcc gtgattatcc
     1561 aaaatcagaa aatgtgcacg aggccgcctg gcggctcggt cttctgctca cccgtgcggg
     1621 aaagacggag gaggctgaaa aagtctatag gacttcactg tcccgcaatc cgcctgaaga
     1681 atggcgggac cggttgagaa tcccgctggc cgcgcttctg cagcagcgcg gacaggagga
     1741 cgagcctgtg gcgcttcttc agcctttgcc tgcaaaggcc gcaatggatg acatgccgcc
     1801 cgccctcatg cactggcttg cgggccgctt gctggcgaag aatgcttttg ccgaagcgga
     1861 aaccattgcg ctggcgcttg caaaacgcaa ggatgcggcc ggctggacgc aggatggatg
     1921 gcatctcgcc ggccgcgccg ccgttgccct gaaaaaaact gcccgggcgc gcgaagcctt
     1981 taaaactgcc gctgaaatga cgggcggcga cacccgcact tccgccgatg ccgccctcca
     2041 gtggggcctg ctggccattg aagacagcgc gaccgatgag gcgttcggcg ctttcacgct
     2101 tgccgcagag cgctcccccg gcgacgacct tctggaaatc cgcgcacgca gctattatgg
     2161 tcttggccgc gttgccgagg tgaaggatga ctgcgatgcg gcctcgcgct actatttgag
     2221 cgtcggactt ctctttgacg aaccttcgct ggtgcctgaa tgtttgtggc gcgccgcgga
     2281 atgcctcgga aaacttcagc gaaacgacga aaagaacctg accctcaagg aactgcggga
     2341 acgctatcct gacagcgaat gggcaaagaa ggaactgccg taaatgaaag aacaggactg
     2401 ggacatcaag gcaaggtcag aggcgtgctc aaaatgcgag catgagtatg aagatcagga
     2461 gcccttctat tcgaaactga cctttgaaga ggacggttat caccgctctg atttctgcga
     2521 gaaatgctgg acggatgaag tgcaggccag cgccctcagc cgctggcgca cgatctatcg
     2581 ctcccccgcg ccgccgccgg aaccggcggt gaaaaaggaa accgccgaat ctctgctgcg
     2641 tgaactgatc gagactgacc gcatggagca ggccaatacg atatatattc tggcggtgat
     2701 gcttgagcgc aagcgcattc tggtcgagcg cgatgtgcag gagcgcgagg acggcacgct
     2761 catacgcgtt tacgagcaca agaagaccaa cgaagtcttc atgattcctg atccgcgtct
     2821 caaactggcc gaacttgaaa cggtgcaggc ggaagtgatt gccatgctcg atggaaccgc
     2881 ccgtgccgcc cagcaggagc tttcagcgcc ggttggcggc gaacccgcgc cggaagctgc
     2941 acctgagaac tccccgccgg aagctccgcc ggcttcctga tttatgcttg atgtcaaaat
     3001 aaccggaggt cttgttttcg acggaaccgg cgcgtccggc cgttccgcgg atattggtat
     3061 cacgggtgat gccattactg aaatcggcga cctgtcctcc gctcctgccg gaaagatcat
     3121 tgatgcccgt gggaaatgcg tttgtcccgg attcattgat gtccactcgc attccgatgc
     3181 gtatctcctg atccagccgg atgcaatgag caagattttt cagggggtca ccactgaaat
     3241 cgtcggtcag tgcggcgcat cggcgtcgcc gctgcagggg gaatataaaa tgccctccga
     3301 ctggcgcgac aagaaatatc cgaaggaatg gcataccgcc tccgaatacc gcgccgcgct
     3361 gcagtcagtt gtgcccgccg tgaatgtcgg attccttacc ggacacagcg ccgtccgcgc
     3421 cggtgtgatg ggttatgagc cgcgtcaggc ctcggccgca gaacttgcgg cgatgaggca
     3481 gcttttggag aagagtttga atgaaggttc acgcggtttc agcaccggcc tggtctatcc
     3541 ccccggaagc tgcgccgatc cgtctgaaat ctgggcgctg gccggcatag tcgcaaagca
     3601 taacggcatt tatgcctcgc atatgcgtag cgagagcgcc ggacttatcg gggcggttgc
     3661 ggaaaccatc gaagtatgcc gcagaacggg tgtgagtacg caaatatcgc atctgaaaac
     3721 tgccggtccg gcaaactggc cgaagattga ccgcgttctt gaaattatac ggtcggcccg
     3781 cgctgaaggg cttccggtca tgtccgaccg ctatccgtac acggcctctt gcactgatct
     3841 ggacatcgtt ctgcctgcct gggcttcaac cggcggcccc gatgcggtgc ttggtcgcat
     3901 ccgcgattcg gcgacccgcg caaaaatcct ggctgaactg aaaaaaagcc gcgcggatgc
     3961 cgactgggaa cgcatcatga ttggttcggt cagccatccg gaccacgcga acttccccgg
     4021 cctgcggctg ccggaaattt cggaacagat gaaagttcat cctgcggaag ccctgctgaa
     4081 aattatcgaa aaggatgaac tcaaaaccgg cggcatattc ttcgggatga gcgaagacaa
     4141 catgtggcgg attctttctg agccttatgt catgatcgga agcgatgcct ccatccgcgc
     4201 tccggcgggt ccgcttagca aagatcatcc gcatccgcgc gcctacgggg ctttcccgcg
     4261 cttcattcgc aaggtaattg atgaaaagcg catgccgctt gaagaagctg tccgcaaaat
     4321 gaccagtctg cccgcagagc atttcaaact gaagggcagg ggccggctgg ccaagggctt
     4381 caatgccgat attacggtgt ttgatccgga gaaaatcgcc gacaaggcca cctttgccaa
     4441 accccatcag cttgcggcgg ggatgagtgc ggttcttgtt ggcggatgcg tgacacttct
     4501 tgacggaaaa ctaagcggtc agcgttcagg ttctgtattg gccggatagc atcctttgca
     4561 tgggatcaca tgccgcacgc cgccgcgcgg attttcaaca tcgtttttgc gtcgcgggat
     4621 caggtgcaca tgcgcatgga aaatggtctg gcccgccgct ttgccgtcgt tgatgcccaa
     4681 attgaatccg tcaatggtct tgtcttgttc cagcagtcgg gcggaaagtt tctttgccag
     4741 cttatgaacc gagagccact catcttccgt cagttcgcga aatgaggcga tatgccgttc
     4801 ggggataatg agaatgtggc cctcggaaac ggggaatccg tcgtaaaccg catagcagct
     4861 tccttcgcgc gcaattacgc gcgcggaatc catctcacaa aaaatgcagg aacggttctt
     4921 catatcagaa agaaaaatgg tggcgagagg cggaatcgaa ccgtcgacac aaggattttc
     4981 agtcctctgc tctaccagct gagctatctc gccaccagat tcttggtttc cagcagtctc
     5041 ggcatagtac gtattcaggc cgctttgtca ataccatgcg catttcctgt tgggtttttc
     5101 tgttgtgccg cgggcggcgt tcacccgcgt cttgacgcgt tcggttctgc tcccgcgaaa
     5161 aagtgaagag ttgacattat ttctgcatgt ttccggcccg ggatgctatg gtggagagat
     5221 taacagcaaa aaaacgaaag gaaacaccag aatggccaag ccgaattatc agtttgaaaa
     5281 gcgcagcagg gagctggcaa aaaagaaaaa gaaggaagaa aagagactgc gcaaacaggc
     5341 agaaaaggat gcccggagca cagcggatgc gccggtgtcg tccagccaat gaaacgcccc
     5401 ctggaccaac ctgaatattc cggcgaaaaa cacttgtgtt ttgcctgatt tcgcgtaggg
     5461 tttgttcgcc cgttaagaga gacaggtcgt gagtggtagg ttggctctga ccccgcaagt
     5521 tgcaggttgg tgtctgatcc gaaatccgaa caactcggaa acagtcaccc aattctggct
     5581 cgcgggcaaa aggaataaga aaatgggtac gaaactgtat gtaggaaacc tgtcatttga
     5641 aacgactgaa gatgacatca gggatctttt ttcgagctgc ggcacggtca cgagctgcaa
     5701 cctcatcatt gacaagttca cgagcaaatc ccgcggtttt gcttttgtgg aaatggcttc
     5761 tcaggccgaa gccacaaagg caatttctga aatgaacggg aaggaagtgt ccggccgtgc
     5821 gctgacggtg aatgaggcca ggccgcgcga agagcgttcc ggcggcggtg gcggcggata
     5881 ccgcggcggc aatgaccggc gtggcggcgg cggcggacgc cgcgagcgct actaaaaact
     5941 ctccagcgca tcggatcatt tactgagccg ggcgcgctga ctttgggcgg cccccgtgaa
     6001 aacgggggcc gcttttttat gccggggtcc cggaagaagg attgcttctg ccgccgggtt
     6061 gatttaatgt gctgacaatg cgctgtgtga gcgctgaacg gcccaacaag gatcatagtg
     6121 tatggcggat atttcggtca gttgtccctc ctgcaatcat gtctgcaaga tatcggaata
     6181 tgtctctgcg gagaccattc cctgcccggc ctgcggagcg cctgtgcaga ttccaaccac
     6241 ctctctgcca agcgaggcgt ccatccagag cacgctccgc ctgaagaaga acttcaagga
     6301 ggggctgggg gagaaaaagg cggcgcctcc tcctccccgg aaagaaatca aacgttcttc
     6361 ctcgatggag aagattcatc aggtgaagga gaagccatcg gggccccgcg ccatctgggg
     6421 cgtactgacg cttctggtgc tgggcggggg gatgttctat ctgctctatt cgctgggcgg
     6481 gaatccggat tatctgaagt attacctgtg gatccgaaac gggaccggtt tgctggcgct
     6541 gggcctggtt tatctttccg ctttcaggga aagtcacatt cagggcctgc tgacgctgct
     6601 gatcccgccc tatgccctgt actatgtcta tgcgcgggcc gactcctatg tgctgcgcgc
     6661 gatgttcacg gcccttatca tcgcgctggg ggctgaattc aagtatatgc gcagcgattc
     6721 gcttctgatc cgcttcaacg attccgtggc ggccttcatc caaagcggtg aagacctgat
     6781 tgatgcggca aacaaggaag tggaaaccac agacgccagc aagaagttcc gctaatacgt
     6841 cttcccgccc cgcgggaacc ggtctcgagt tcagaccaga tcgatcaggt cgctgatgcc
     6901 gccgtcgtcg ggtttctttt ccggggcttt ttcggcgggc ttctgttccg gctctgctgc
     6961 cggcgcctgc tgtcgttcgc gccttgggcg gttccggttt cggcggtgcc ggtcctgtga
     7021 ggggttccgg ctgtcggaat ctccctgtcc gccgttgccg gaaagcgcct ttttgaaggt
     7081 gctggcccgc agcagggctg ctcctgcctg gcggacatca tcctccaccg accggttgga
     7141 tgttaccgca acgacccctt tgcggccata gcgtttcgcc agtttcgctg cgagggccgg
     7201 cagttcctga acagatgccg catagctcac ttgcacattc ctgaaggact cgttctccgg
     7261 aacggaccgc agcggctcgc cagtcagcat ggcgtgaaca gtgatcttct ctttctccgc
     7321 atagcgcgag agcgaatgaa gaatgtcgat ctgcaccttg ggaggcaggc ggttgctgcc
     7381 gccgccaatg gacatcacat cggccaaaag tattttggct gcgggagctg cgtttccgct
     7441 ccgcttgccg aacaacctgt caaaaaagct catctgtact ccacttcgtt tattcctctg
     7501 atcttcagca ggataaggtg cacacgttat atcatttccc ttgtgtaatc aagcggcgga
     7561 tgccgcgtcc ccccgatttt ttgccgcccc ggatgattat gcgttgtatg ggccggggca
     7621 aaaggctaaa ctctccgggt aaatgacacg ctccggcgtc tcggcggggc ggtcatgagg
     7681 agaaacagag gttttgtgat gagtgcaaca gttaccagtg gcgttgtgca gtccgtagtc
//
LOCUS       DAOQWR010000126         7648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_9980_length_7648_cov_4.892137, whole genome shotgun sequence.
ACCESSION   DAOQWR010000126 DAOQWR010000000
VERSION     DAOQWR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9980_length_7648_cov_4.892137"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..343
                     /locus_tag="PKK36_09525"
     CDS             20..343
                     /locus_tag="PKK36_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94829.1"
                     /translation="MGQELATANSTHVVLIIGLNAYHYWFGFDEGGVSDGRFYCEIWY
                     LKYPPPTATYSGTFLYEEGWGSKAPARKIRKRGARASKPIRSAPRENSMRLKTEWPAP
                     RKLRR"
     gene            complement(350..2494)
                     /locus_tag="PKK36_09530"
     CDS             complement(350..2494)
                     /locus_tag="PKK36_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94830.1"
                     /translation="MKNNRFAVVILSILLFVMTFALFSPALSFSLVSFDDPVFITHNP
                     IIFNGFSWEAIRSAFTKLHGDECMYVPLLWISYLLDLKFFGASSANPWGFHFTSIFFH
                     ALNSVLLYLLLYAFCRKPWRALFFAAIWALHPLRVESVAWVTSRKDVLSTFFALLCIL
                     AYVQAWTAKLRENGSARAVSALHVTAFVFFILGLLVKPMLVTIPFLLLLLDIWPLRRL
                     EGMPRASLSPALLRVLREKIPYFLGAAGAAMAVYLTQTKAMSTVPLWVRLYCIPSNYL
                     FYLYKFLYPVRLFAMVPRQPVAPAYFLLSTAFLGSLTAWIWMRRREQPHMIIGWLTFL
                     GLLFPVIGIVVIGIYPVADRYSYFPSIGLSIALLFAFSSKALPNRGARAGRNGLAVCV
                     LLTLALLTLRLLPSWKNDRSMYAHIARLHPDHYAAIHYQAREELFANGNIVKADRMAS
                     QMLEIKPCVSFGLVLKIICLSQLQSTEAALEFARENYPPCDNLGNPGIYEGYLTTLNF
                     FAGQYEQANHFMEETFRRSVFEPKTQEQLHAIAMLLAHQQGNDAAALAYAAKITALKD
                     KKRLLPEDYFLSYTALWSSGLYVQALPFFQKLAQSSAGRPDILNNIAWLLATTSGSPA
                     DPEEVIQMVNKALEASPGHPVILDTLSVAQANAGDFDAALRTAEKVAAFLKSSTATDA
                     PQMLRKVEKRIALYRERKPFREYSATRLLYAP"
     gene            complement(2491..4671)
                     /locus_tag="PKK36_09535"
     CDS             complement(2491..4671)
                     /locus_tag="PKK36_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94831.1"
                     /translation="MKFVGTRRIPLQAMLIFCLVFALFAPSLRYSLVDMDDMTYLLNS
                     ALVMEGFSCDHVQTVFTSFQHGMYSPLLWLSFGLDAAIFNASPVNPWGFHFTNVLLHA
                     INAALLFLLLDFFCKKPWRAFFFAALWAVHPLRVESVAWVSERKDVLSGFWGLLCIWA
                     YVQAWHRRKDSDRGGRLAPTLTVASLVFFALGLLAKPAIVPIPFVLLLLDYWPLRRID
                     ISLPSIKRCAPRLLTEKVPFFICAIGASLAATAAHKVIYGLAEVPLSTRIFNVPVHYA
                     FYLIKTAAPWRLSPLYPDLPESPFFIAIACILLLAVTAWVWQCRDRHPSRLAGWLFFL
                     GFFAPAIGLIRFGAQSIADRFTYLPSIGLSIGFLFVWPDNAKHAASARILRVLSALAI
                     IVIFTVSTLRLLPAWRSPSSLSEHILSIIPDSPTGLEMHAFQLIYQSGNFHEASAVFD
                     RIIAQGSFNHNVLSGKARCLAELQGPETAIAFFLRMPETGNPYDMRVWDIARYSLMQG
                     KYNDAITHARRALQLRPDNLSHPVYIHLLIMTAAYKQGNTALALTHARQLPAYAGRTS
                     LEFADLLPYYLHEWIECHRSDAGNYFRQLIEQQPDNTGLLNNIAWGLATANWSPVPSD
                     ETLDMARRVCDSMPRPNPGALDTLAAAQANAGDFPSAIQTIQEAIDLLPDRDDPHVAE
                     FRKRLMSRLTLYRQQKPHREEAFSRLMAAQFGKGLPVTNKKDAP"
     gene            complement(4668..6875)
                     /locus_tag="PKK36_09540"
     CDS             complement(4668..6875)
                     /locus_tag="PKK36_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94832.1"
                     /translation="MNGISRGGKSLRALIAGLFLFTAVFAVFSPSLEYGLVDLDDITY
                     ISNNTAVLEGLTASSIRQAFSPNNMSATMYMPLLWISYMADVEWMDASPAQPRGFHFS
                     NVLLHALNSVLLFVLLLALSGKPWRALFFTALWAFHPLRVESVAWVTERKDVLSGLFA
                     LLCIGSYLRSRAVPISGRTRLFFTGVSLFCYGLGLLVKPSLVSIPFILLLLDFWPLNL
                     VELTGRSVREKATRLLLEKVPYFAAAGLAAIGTVWGHHAFSGEIQVSLLLRLLSVPLT
                     YGFYLLKIFFPRNLTVLYPHFSIWLNPDRLIALSVVSAVFLAGMTICAWRLKKRFPGG
                     LVGWLWFLIILIPVCGIIPIPSNDVADRFSYLPAAGLSIALLPLLSSQSRFLRRLKWV
                     QPVLACLILSGLIWLSLRQLPVWESTASLYNRVLEVFPSHATALKNRASQLIRSTGNF
                     KEADRLISKALEAEPHHWESHFTKAQCLSELEGPAEALRHLQQIIPPVSRFTYAYWQR
                     DLARYELMLGKYDEAIRHADQARAQVPPQDLSQIPFILLAMTAAYEKGDMPAALNYAR
                     EFAPYQSKTALELADLLPHAIFQWVAGYRQDAYAYFQRLVRNYPDRSDILNNVIWGLA
                     TANWSPADPQEVLDMAERLRDMVPEPHPGILDTLAAAQASAGDFEAAVLTLKNALALF
                     PASPGPDLVQFRERLAARLALYEQQRPYREEAFTRMYVTYFGPPVKLREQKGP"
     gene            7038..>7648
                     /locus_tag="PKK36_09545"
     CDS             7038..>7648
                     /locus_tag="PKK36_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94833.1"
                     /translation="MDTIDNANLDSVSPLVCVRTGWLRDITRAGWQAWIRMLLIFCMG
                     LAFLLPRYIDYIRSGLDPSWVYVINAANRSGWTFGKDILFTHGPLGFLSSTCGVGRNI
                     EITLVILCIFLLVQMRLLCRFVFSKSAEERPILAVMILSCLLLWAIPRPRFDSYLLYM
                     VLLLLSVAWTSRNPQRCYGWACVLTTLQLFMVFTGALSCCSAIG"
BASE COUNT         1854 a         1950 c         2405 g         1439 t
ORIGIN      
        1 gagcgggaac atttccgata tgggccagga actggcgacg gccaacagta cgcacgtggt
       61 gctgattatt ggtctgaatg catatcatta ctggtttggt tttgatgaag gaggcgtctc
      121 cgacggccgc ttttactgcg aaatatggta tttaaaatat ccgccaccta cggcaacata
      181 cagcggaaca tttctttatg aggagggctg gggatcaaaa gcgccagcca gaaaaatccg
      241 gaagcggggg gcaagggcgt cgaagccgat tcgttctgct ccccgggaaa attcaatgcg
      301 cctgaaaaca gaatggcctg caccacgcaa attgcgccgg taacctgaat cacggggcgt
      361 aaagcaggcg ggtggctgag tattcccgaa aaggcttgcg ctcccgataa agagctatgc
      421 gtttttccac tttgcgcagc atctgggggg catcggtggc cgttgaagat ttgaggaaag
      481 ccgccacctt ttccgccgtc cgcagggccg catcaaaatc gcccgcattg gcctgcgcca
      541 cggacagcgt gtcgaggatc accggatggc cgggagatgc ctcaagcgcc ttgttgacca
      601 tttgaatgac ctcttccgga tccgcaggag agcccgacgt ggtggccagc agccaggcga
      661 tgttgttgag gatgtcgggc cgaccggcgg aactctgcgc gagtttttga aagaacggaa
      721 gcgcctgaac atacaaaccg ctggaccaca gcgccgtata ggacaggaaa taatcttcgg
      781 gaagcagccg ctttttgtct ttcagggcgg tgatcttggc ggcatacgca agcgcggcag
      841 cgtcatttcc ctgctgatga gccagcagca tggcgatggc gtgcagctgt tcctgcgttt
      901 tcggctcaaa cacgcttcgg cgaaaggtct cctccataaa atgattggct tgctcatact
      961 gcccggcgaa aaagttcagt gtggtcagat aaccttcata aatgcccgga ttgcccagat
     1021 tgtcgcaggg cggataattc tcccttgcaa attcaagcgc ggcctctgtt gattgaagct
     1081 gtgagagaca tatgattttg agcaccaggc cgaaggaaac gcagggtttg atttcgagca
     1141 tctggctggc catccggtcg gccttgacaa tattcccgtt cgcaaacagc tcttctctgg
     1201 cctgataatg aatagcggca taatgatccg gatgaagccg tgcgatatgc gcatacatgc
     1261 tcctgtcatt cttccacgac gggagaaggc gcagggtgag gagggccagt gtcaggagca
     1321 cgcagaccgc cagcccgttt cgtccggcgc gcgccccgcg attcggcagg gcctttgagg
     1381 agaaggcaaa cagcagggcg atggaaaggc cgatggaggg aaagtaggaa tagcggtcgg
     1441 cgacaggata tattccgatt acaacaatcc caatgacggg aaacagcagg ccaagaaagg
     1501 tcagccagcc gatgatcatg tggggctgtt cgcggcgacg catccatatc caggcagtga
     1561 gagagcccag aaaggcggtg gacagcagga agtacgccgg ggcaacaggc tggcgcggca
     1621 ccatggcaaa cagacggacc ggatagagga atttgtagag atagaaaagg tagttggagg
     1681 gaatgcagta gagccgcacc cacagtggga cagtggacat agcttttgtc tgggtgagat
     1741 aaacggccat cgccgcaccg gccgcgccaa ggaaatacgg gattttttcg cggagcacgc
     1801 ggaggagtgc gggggaaaga gaggcgcggg gcatcccctc cagccggcgc agcggccaga
     1861 tgtccagaag cagaagcaga aacgggattg tgaccagcat gggtttaacc agcagcccga
     1921 ggatgaagaa cacgaacgct gtcacatgca atgcagagac cgcgcgtgca gatccattct
     1981 ccctgagttt cgcggtccag gcctgcacgt aggccagtat gcacagcagt gcgaagaagg
     2041 tggacagcac atccttgcgc gaggtgaccc atgcaaccga ctcaacccgc aggggatgca
     2101 gcgcccagat ggctgcgaaa aacagggccc gccagggctt cctgcagaag gcatacagca
     2161 ggaggtacag cagaacggaa ttgagggcat ggaaaaatat gctggtgaaa tgaaatcccc
     2221 agggattggc ggaacttgcg ccaaagaatt taaggtcgag cagataagat atccatagaa
     2281 ggggcacata catgcactcg tccccatgaa gttttgtgaa ggcgcttcgt atggcctccc
     2341 atgaaaaccc gttgaagatg atggggttgt gcgtgatgaa caccggatca tcgaaggaga
     2401 caagtgaaaa tgacagcgcc ggggaaaaca gggcgaacgt catcacaaag agcaggatgg
     2461 agaggataac aactgcaaac cggttgtttt tcatggggca tctttcttgt ttgtgacagg
     2521 cagtcctttg ccgaactgcg cggccatcag ccgggagaag gcttcctccc gatggggctt
     2581 ctgctgccgg taaagtgtca ggcgggacat gaggcgcttt cggaattccg cgacatgggg
     2641 atcgtcccgg tcgggaagca gatcaatggc ttcctgaatt gtctgtatgg cggaagggaa
     2701 atcacccgca ttggcctgcg cggccgccag ggtgtcgagg gcgccgggat tgggccgggg
     2761 catggaatcg cacacccgcc gggccatatc cagagtttca tcggagggga ccggagacca
     2821 gttggccgtg gccagccccc atgcaatgtt gttgagcagg cctgtgttgt cgggctgttg
     2881 ttcaatgagc tgccggaagt aattgcccgc atcagaccgg tggcattcga tccactcgtg
     2941 gagatagtag gggagcaggt ctgcaaattc cagcgaagtc ctgcctgcat aggcgggaag
     3001 ctgacgggca tgggtcaggg caagggcggt attcccctgc ttgtatgccg ccgtcatgat
     3061 gagcagatgg atatataccg gatgagagag gttgtccggt ctgagctgca gcgcgcgccg
     3121 ggcgtgtgtg atggcgtcat tgtacttccc ctgcatcaga gagtagcgcg caatatccca
     3181 tactcgcatg tcatagggat tgccggtctc gggcatgcgg aggaaaaaag ctatggcggt
     3241 ctcgggcccc tgcaattcgg caagacatcg ggccttcccg gagagcacat tgtgattgaa
     3301 ggagccctgt gcaataatgc ggtcaaaaac agcgctggcc tcatggaaat tcccggactg
     3361 gtaaatcagc tgaaatgcat gcatttcaag gcctgttggg ctgtcgggta tgatggaaag
     3421 aatatgctca gacagcgatg agggcgaacg ccatgccggc agcagtctga gggtgctgac
     3481 ggtaaaaatg actataattg cgagcgcgga cagaacccgg aggatgcgcg cagatgccgc
     3541 gtgttttgca ttgtcgggcc agacgaatag aaatccgatg gagagcccga tggaggggag
     3601 ataggtaaag cggtccgcga tggactgagc cccgaacctg atcaacccga tggccggcgc
     3661 aaagaacccc agaaagaaca gccagcccgc cagccggctc ggatggcgat cccggcactg
     3721 ccagacccac gctgttacag caagcaggag gatgcaggca atggcgatga agaacggaga
     3781 ttcggggaga tcgggataaa ggggggagag tctccagggg gcggcggttt ttatcagata
     3841 aaaagcataa tggacgggga cattgaatat tcgggtggat aacgggactt cagcgagccc
     3901 gtaaatgacc ttgtgggccg ccgtcgcagc cagtgatgcc ccaatggcgc agatgaagaa
     3961 gggaactttc tctgtcagca gccgtggggc gcagcgtttg atggatggga gcgagatatc
     4021 aatgcggcgc aggggccagt agtccagcag aagcagcacg aaaggaatgg gcacgatcgc
     4081 gggtttggcc agaagaccca gtgcaaaaaa cacaagcgag gcgacagtga gggtgggggc
     4141 cagccgccct ccccgatcac tgtcttttct tcgatgccac gcctgcacgt aggcccagat
     4201 gcagagcagc ccccagaatc cggacaggac gtctttgcgt tctgaaaccc atgcgacgga
     4261 ttccacgcga agcggatgca cggcccagag cgcggcaaag aaaaaggccc tccagggctt
     4321 tttgcagaag aaatcgagca gaaggaaaag aagggccgcg ttgatggcat ggagcaggac
     4381 gttggtgaaa tggaatcccc atggattgac tggcgaggca ttgaatatcg ctgcatccag
     4441 gccgaaagac aaccaaagca ggggggaata catgccgtgc tggaaggatg tgaagaccgt
     4501 ctggacatgg tcgcaagaga acccctccat gaccagcgca ctgttcaaga gataggtcat
     4561 gtcatccata tccaccaggc tgtaacggag cgacggggcg aagagggcga agaccaggca
     4621 gaaaattagc atggcctgaa gcggaatacg gcgtgtgcca acgaacttca tggtcccttc
     4681 tgctccctta attttacggg ggggccgaaa tatgtgacat acatgcgggt gaaagcctct
     4741 tcccgatagg ggcgctgctg ctcatagagc gcgagtcggg ccgccagtcg ttcccggaac
     4801 tgaaccaggt cgggtccggg actggctgga aacagcgcca gagcattctt cagcgtgagg
     4861 acggctgctt caaagtctcc ggcgctggcc tgggcggcgg ccagtgtgtc cagaatgccc
     4921 ggatgaggtt cggggaccat gtcgcgaaga cgttcagcca tatccagcac ctcctgcgga
     4981 tcggccgggg accagtttgc ggtcgcaagc ccccagatca cgttgttcag aatatccgat
     5041 cggtccggat agttccgcac aagccgctgg aaataggcgt atgcatcctg ccggtagccg
     5101 gccacccact ggaagatggc atgcgggagc aggtcggcca gttccagcgc ggtcttcgac
     5161 tggtatgggg caaattccct ggcatagttc agggccgccg gcatgtctcc tttttcatag
     5221 gcggcggtca tggccagaag aatgaacggg atttgcgaca gatcctgcgg cggcacctgc
     5281 gcccgtgcct gatcagcgtg ccgaatggct tcatcatact tcccgagcat gagttcatag
     5341 cgggccagat cccgctgcca gtatgcatag gtgaatcggg acaccggcgg gatgatttgc
     5401 tgcagatgcc gcaatgcctc cgccgggcct tccagttcgg acaggcactg ggctttggtg
     5461 aagtgggatt cccagtgatg aggctccgct tccagcgcct ttgatatcag ccggtccgcc
     5521 tccttgaaat tccccgtgct gcggatcagc tgcgatgcgc ggttcttgag ggccgtggca
     5581 tgactgggga aaacttccag cacccggtta tacagagacg cagtgctttc ccacaccgga
     5641 agctggcgca gagacagcca gatcaggccg gaaagaatga gacatgccag aaccggctgt
     5701 acccatttca gtctgcgcag aaatcgggat tgagatgaaa gaagaggaag cagtgcaatc
     5761 gacaaaccgg cggcggggag atagctgaag cgatcagcaa catcattgga agggatgggg
     5821 atgataccgc atacagggat cagtatgata agaaaccaga gccagcccac cagcccgccg
     5881 ggaaatcgct tcttcaggcg ccatgcgcaa atggtcattc ccgccagaaa aaccgcagag
     5941 acgaccgaga gtgcaatcag ccggtcagga ttcagccaaa tggaaaagtg cggataaagc
     6001 acggtcaggt tgcgggggaa aaaaatcttc agcaggtaga atccataagt cagagggaca
     6061 gacaacaggc gcagcaggag cgatacctgg atttctccgc tgaaggcatg atgaccccag
     6121 acggtgccga ttgccgcaag cccggccgcg gcaaaatagg gaaccttctc caggaggagc
     6181 cgggtcgcct tttcccgcac ggagcgccct gtgagttcaa ccaggttcag cggccagaaa
     6241 tcaagcagga gcaggataaa cgggattgaa accagcgagg gtttgaccag caggcccagg
     6301 ccataacaga acagcgagac cccggtgaaa aacaggcgtg tgcggccgga aattggaaca
     6361 gctcgcgatc gcagatacga accgatgcac agcagcgcga agagcccgga gagaacgtct
     6421 ttccgctcgg tgacccacgc cacggattca acgcgaagag ggtgaaatgc ccataacgcc
     6481 gtgaagaaca gggcgcgcca cggcttcccg gacaacgcga gaaggagaac aaaaagcagg
     6541 acggaattca gcgcgtgaag aagcacattg gagaaatgaa acccccgcgg ctgtgccggc
     6601 gacgcgtcca tccattcaac gtccgccatg taggaaatcc acaggagcgg catatacatg
     6661 gtggcgctca tgttgttcgg ggaaaaggcc tgccggatgg aggatgcggt caagccttca
     6721 aggacggcgg tgttgtttga aatgtaggtg atatcatcca gatccaccag gccgtattca
     6781 agcgagggag agaaaaccgc aaaaacggca gtaaagagga acagacccgc gatgagggcg
     6841 cggagagact ttccgccccg ggaaattcca ttcatatcct gcttcatgaa cgacaatata
     6901 ggggccgaag aaggcgggat tcaaggcgaa aggggaagaa ggggagattc gacggcacat
     6961 aaactgctcc aggccggctt gagagaagag gcttgaacag ggagaggtgg tccgttatct
     7021 cttggcgagg attgggcatg gataccatag acaacgcgaa tctggacagt gtttctcccc
     7081 tggtttgtgt gcgaacaggg tggttgcgcg acataacgcg ggcaggctgg caggcatgga
     7141 tccggatgct gctcattttc tgcatgggtt tagcctttct cctgccccga tatattgatt
     7201 acatccgcag cggcctggac ccctcgtggg tctatgtaat caatgccgca aaccgatctg
     7261 gctggacatt cgggaaggat atactcttca cccatgggcc attgggtttt ctttcttcca
     7321 cctgcggggt agggcgcaat attgagatca ctcttgtgat tctttgcatt ttccttttgg
     7381 tccagatgcg gcttctgtgc cgttttgttt tctcaaaatc tgcggaagag cgtccgatac
     7441 tggctgtcat gattctatcc tgccttcttt tatgggccat cccgcgcccg cgcttcgaca
     7501 gttatctgct ttatatggtt ttgctcctgc tttctgtcgc ctggaccagc agaaacccgc
     7561 agcgctgtta cggatgggcc tgtgtgctga caacgctgca gctcttcatg gtgttcaccg
     7621 gagcgctgtc ctgctgcagt gcgatcgg
//
LOCUS       DAOQWR010000127         7600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_10060_length_7600_cov_5.447979, whole genome shotgun sequence.
ACCESSION   DAOQWR010000127 DAOQWR010000000
VERSION     DAOQWR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_7600_cov_5.447979"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>323
                     /locus_tag="PKK36_09550"
     CDS             80..>323
                     /locus_tag="PKK36_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94834.1"
                     /translation="MNTVFEELKYFLDEDFPKSCNCGESFYSMDDFIERTTEVLDTEM
                     GICNTCGEGGIMVNMVRQCRCGARMMIAFSGSIQASV"
     assembly_gap    324..333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <334..569
                     /locus_tag="PKK36_09555"
     CDS             <334..569
                     /locus_tag="PKK36_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94835.1"
                     /translation="RDRTEKGAQRRLLFRDLMTFFTEAGMDPLKARNELIRVVHGQQS
                     EEILKILRRAKVDKLDSLSLSIQLRMLKDFLRC"
     gene            complement(627..1796)
                     /locus_tag="PKK36_09560"
     CDS             complement(627..1796)
                     /locus_tag="PKK36_09560"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR94836.1"
                     /translation="MTAAAGYDVIVLGAGASGLMCAAAAGARGRRVLVLEGSPKPGNK
                     ILVSGGGRCNFSNVNAGPEHFISANPHFCKSALSRFSSHEICALLDRHHVRWHEEDGG
                     RLFCRDGSKKMLDALLAECREAGVVIRTDAGIREIGAGFRVAAECGEFHAPSLVIATG
                     GRSWPQIGASGFGYDVARQFGLKVISPRPALVPLVFDAAGRRAFGGLAGLSVRVRVSC
                     GDAVFEDDMLFTHRGLSGPAVLQISSYWREGEPVAVDLLPQMRLDEAFPVWKKDHPRT
                     ELKNLVGRHVPRRLAHCFLEGLPPVNQMGEGELKKISERFHNWKIVPAGTEGFEKAEV
                     TRGGVDTTELSSRTFEARKVPGLFFIGEVLDVTGWLGGFNLHWAWASGRCCGQCC"
     gene            complement(1793..3064)
                     /locus_tag="PKK36_09565"
     CDS             complement(1793..3064)
                     /locus_tag="PKK36_09565"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNR94837.1"
                     /translation="MKKPKYEVHVLGCKVNQYDAQQIRELLNLSGAVPAGDSAPDISV
                     IHTCGVTAAALQKSRQAVRAAQRSNPGGFVIMTGCCCALPDFTSPPEAAACIAPHEHW
                     LAELRDVLNRLPLALSPVDIPEHSERFGIHSFSSHTRAFLKIQNGCTVGCSYCIVPRL
                     RGYLYNKPLPDILAEARALAAAGHREIIVSGVSVGLYNGLARVMRALCDEGTVPRLRL
                     SSLHPNELTDELLEVWASYKCMLPHIHLPLQSGADRILKLMRRSYTCSDFLRAAAAVR
                     AVLDNPAITTDVIAGFPGETDRDFRDTVEFCRAAGFSRMHVFPFSPRPGTAAEKMPDR
                     VAPGEIRTRAAKLRALGRDLTRKYCGAFVGKDVEVLVEQRSEKGICSGYSEHYVPVEF
                     EGSAADKGTICRVRADRVVRGQTLHAVRRAP"
     gene            3091..3825
                     /locus_tag="PKK36_09570"
     CDS             3091..3825
                     /locus_tag="PKK36_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNR94838.1"
                     /translation="MTSHTAEFRFYGELNNFIEPARRGQTRPCAFDGAPSVKDAIEAM
                     GVPHTEVDLILADDQAVDFSFHLRDGCRVAVYPHFFTPCMAPEGRLHRPPACPICFVL
                     DGHLGKLARLLRLAGYDALYRNHCDDAELASISAEQKRVLLTRDRGLLMRRCVQQGCF
                     VHATDPVAQLREVMRRYELNTARALTRCMVCNGLMAPVEKTRIMDRLPDNTRKNCSVF
                     FQCSGCGKIYWEGSHYARLRRLVDSL"
     gene            3865..4350
                     /locus_tag="PKK36_09575"
     CDS             3865..4350
                     /locus_tag="PKK36_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNR94839.1"
                     /translation="MKTTFAMLVISLMLSAATVRAEEETAQTLAEELLVLMDVQSSIQ
                     KSFDAVKQMQATQFASMAGDRKEQALAMQQKMMDLIAGEMSWDKIKTDYIGIYAETFT
                     ADELKGIIDFYKTPVGRKFIEKQPEIMEKSMRVTQKQMMTLMPKIQALALEAAGMPPA
                     Q"
     gene            complement(4351..6021)
                     /locus_tag="PKK36_09580"
     CDS             complement(4351..6021)
                     /locus_tag="PKK36_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94840.1"
                     /translation="MKKNISLSELKLLRDSLLAELCPVLEESDARHENVQQILTGCPV
                     ISAPVETAVEALISSSEKLYANLLREEATLYKKVLETRQEQNASSRFGLYQAALPVLA
                     GDRICCCLRTGWMITEPVSGKLLSELEAHGTLSDKELKRAVESLRVYTREEEQQVTAF
                     LRQLRDLLSVLLDESRRADSLAEQLISSERARSLGALTGGVAHHFNSLLSVILGYSSF
                     MLNRLSLPPEASDALEHISEAAQKGRRLTEEMLAFLGSEAEVQATCGVHEILEGVVSL
                     LNSQVHSRVRFTMNLGAKNDEISARPSALHQVIFNLVTNSLDAMDGPGCIMVVTQNTE
                     MRTGNVLRRFIEIGVAEQEGEKSAEEMPLTGDSSAPENASLKISSLFGLVADLEGSVL
                     IDPAAVSGKPRVKVILPTIAQADARAASPDGGDQLKPSTIWVVDDDETFRKMCEFVLS
                     GQGHTVLSLSGANELQDKWTGGATPPDMIIMDFSMPDFNGLEMCQWLRRAGADVPVVL
                     VSGLSANQPDIHRATAMKRTTFLQKPFTFRELTDAVEKALAESLIGGR"
     gene            complement(6028..6873)
                     /locus_tag="PKK36_09585"
     CDS             complement(6028..6873)
                     /locus_tag="PKK36_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR94841.1"
                     /translation="MSDSRVKCLFCGQVFMVDKEKAGASAPCPACGKETFLDMPDDPG
                     RLESYQIKDSSGVTEDSRDCPACGAPVPGNALVCTQCGYNLETGQKKNTRIVRQGTAL
                     SRFAVPVLLMIISGMAVYIVQESRFEKRLEKAREQWEASTARISAAPLPAVSAPPAAA
                     AAEEPAAAAVEAPAPAAPPAEAVVQSPEEEAGVLAQREQRFRDELDKQSPLYPKNESV
                     TLVGKTGRVHRGRLIGLKSDVVVLAVAGGTEEIALNQLSHESRLRCDKTYRDEYIKRR
                     VQPRR"
     gene            7045..>7600
                     /locus_tag="PKK36_09590"
     CDS             7045..>7600
                     /locus_tag="PKK36_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNR94842.1"
                     /translation="MQLDKLTIKSQEAVHHARRLAEQAGHGEIGTPHLVAALIDQEEG
                     IVRPLLQRLGTPPDGLRDQVERLLSKRPRVEGAAVDLYISRDLKQVLDNAFALAEKMK
                     DEYVSTEHLFLAAIEKGSDEFQAIARQAGLTPDAVLKALQAVRGRQRVTDPAPEDKYE
                     AMKKYGHDLGGRIRNTLAPPDRLQG"
BASE COUNT         1607 a         2352 c         2198 g         1433 t
ORIGIN      
        1 ccgttttttc gcttcttttt ataaggcata aatggctcca tcgtgatata aacagaggtg
       61 gagaagcaga ggggaagaca tgaatactgt gttcgaagaa ctgaagtatt tcctggatga
      121 ggatttccct aaaagctgca attgcgggga aagcttttat tcaatggatg acttcatcga
      181 aaggaccact gaggtcctgg acacggaaat ggggatctgc aatacctgcg gcgagggcgg
      241 gatcatggtc aacatggtga gacaatgccg ctgcggagcc cgcatgatga ttgccttcag
      301 cgggtccatc caggcctctg tgannnnnnn nnngacgcga ccgcaccgag aagggcgccc
      361 aaagaaggct gttgtttcgt gacctgatga cgtttttcac agaagccggg atggacccgc
      421 tgaaggcaag gaatgaactc atcagggtcg tgcacgggca gcagagcgaa gaaattctca
      481 aaatactgcg acgggccaaa gttgataaac tggacagcct gtcgctcagc atccagctcc
      541 gcatgctgaa ggattttctc cgctgctgat tctgaagaag aaaacaacca gagggcggac
      601 atctgtgccg cctgcggccg gcatattcag cagcattggc cgcagcaccg tccggaggcc
      661 caggcccagt gcaggttgaa accgcccagc cagcccgtca catccagcac ttcgccgatg
      721 aaaaagaggc ccggcacttt gcgcgcctcg aaggtcctgg atgaaagctc cgtcgtgtcc
      781 acgcccccgc gcgtgacttc ggccttctca aatccttccg tgcccgccgg cacgattttc
      841 cagttgtgga acctctccga tattttcttc agctcgcctt cacccatctg attcaccggg
      901 gggaggcctt ccagaaaaca atgcgccagt cgtctgggga catgcctccc aacaagattt
      961 ttcaactcag tccggggatg atccttcttc cagacgggaa acgcctcgtc caggcgcatc
     1021 tgcggaagaa ggtcaacggc gacgggctcc ccctcccgcc agtatgatga tatctgaaga
     1081 accgcagggc cgctcagccc ccggtgagtg aacagcatat catcctcaaa aacggcatcg
     1141 ccgcacgaca cgcggacccg cacggacaat ccggccagcc cgccgaaggc cctgcggccg
     1201 gccgcatcaa acaccagcgg caccagcgcc ggacgcgggg aaatgacttt caggccaaac
     1261 tgccgggcga catcatatcc aaatcccgac gcgccgattt ggggccatga tctgccgcct
     1321 gtcgcaatca ccagcgatgg cgcatggaat tccccgcatt ccgccgcaac gcgaaaaccc
     1381 gcgccaattt cgcggattcc ggcatcagtc cggatcacca cgccagcctc gcggcactcg
     1441 gccagaagag cgtccagcat tttcttcgag ccgtcgcggc agaacagccg gccgccatcc
     1501 tcctcatgcc agcgcacgtg atgacggtcc agcagcgcgc agatttcatg cgaagaaaag
     1561 cggctcagcg ctgatttgca gaaatgggga ttggcggaga tgaaatgctc cggtccggca
     1621 ttcacgttgg aaaaattgca gcggccgccg cctgacacga gaattttatt tcccggtttc
     1681 ggcgagccct ccagcaccag cacacgacgg ccgcgggcgc cggctgcggc ggcgcacatc
     1741 agaccggaag cgcccgcgcc caggacaatc acatcgtacc cggcggccgc agtcacgggg
     1801 ctctccgcac ggcatgcagg gtctgccccc ggacaaccct gtccgcgcga acacggcaga
     1861 tggtcccttt gtccgcggca gatccctcga attcaaccgg cacataatgc tccgaatagc
     1921 cggaacaaat gcctttttcc gagcgctgct cgaccagcac ttccacatct tttccgacaa
     1981 aggccccgca gtattttcgg gtcaggtccc ggccaagggc gcgcagtttc gccgcgcgcg
     2041 tcctgatttc tccgggcgca acgcgatccg gcatcttctc cgccgccgtg cccggccgcg
     2101 gagaaaacgg aaaaacatgc atccgcgaaa aaccggccgc acggcagaac tccaccgtgt
     2161 ccctgaaatc ccgatccgtt tctcccggga atccggcaat cacatccgtg gtgatggcgg
     2221 gattgtccag gaccgcgcgg accgctgcag ccgcgcgcaa aaaatccgag cacgtgtagc
     2281 tgcgccgcat gagcttcaga atccggtcgg cgcctgactg gagcggcaga tggatgtggg
     2341 gcagcatgca tttgtacgac gcccacacct ccagcagttc atcggtgagt tcattcggat
     2401 gcaggctgga aagccgcaga cgcggcaccg tcccctcatc acacagcgcg cgcatcacgc
     2461 gggcaaggcc gttgtacagg ccgacggaaa ccccggaaac aatgatttcc cggtgaccgg
     2521 cagcggccag ggcccgggct tcagccagaa tatccgggag cggcttgttg taaagataac
     2581 cgcgaagccg gggcacaatg cagtaggagc agccgaccgt gcatccgttc tggattttca
     2641 gaaatgcgcg ggtgtgactg gaaaaggaat gaatgccgaa acgctccgag tgttccggaa
     2701 tatccacggg cgacagggca agcggcagac ggttcaggac atccctgagt tccgccagcc
     2761 agtgctcatg cggggcgatg caggccgccg cctcgggcgg ggatgtaaaa tcgggaagcg
     2821 cgcagcagca ccccgtcatg atcacaaaac ccccgggatt gcttctttgc gcggcgcgga
     2881 ctgcctgccg ggacttttga agagccgcgg ccgtaacgcc gcaggtgtga atgacggata
     2941 tatccggcgc gctgtccccg gccgggaccg cgccggaaag attcagcagt tcgcgaatct
     3001 gctgggcatc gtactggttt accttgcatc ccagcacgtg cacttcatat ttcggtttct
     3061 tcatgattcc cgccggtata cactaaatcc atgacttcgc atacggcaga gttccgtttt
     3121 tacggcgagc tgaacaattt catcgaaccc gcgcggcgcg gacaaacccg cccctgcgca
     3181 tttgacgggg cgccttcggt caaggatgcc attgaggcca tgggtgtgcc gcacacggag
     3241 gtggacctga ttctggctga cgatcaggcc gtggatttct cttttcatct gcgggacggc
     3301 tgcagggtcg cggtgtatcc gcatttcttc acaccctgca tggcgccgga gggccggctt
     3361 caccgacccc cggcctgtcc gatctgcttt gttctggacg gccatctcgg gaagctggcg
     3421 cggctgctcc gcctggcggg gtacgacgcg ctctatcgaa atcactgcga tgatgccgaa
     3481 ctcgcctcca tctccgcgga gcagaaacgc gtgctgctca cccgggaccg ggggctgctg
     3541 atgcgccggt gcgtgcagca gggctgtttt gtgcacgcga cggatccggt cgcccagctt
     3601 cgcgaagtga tgcggagata cgaactgaac accgcccggg cgctcacgcg atgcatggtc
     3661 tgcaacgggc tgatggcgcc ggttgagaag acgcggatca tggaccgcct gccggataac
     3721 acccgcaaaa actgcagtgt gttcttccag tgcagcggat gcgggaagat atactgggaa
     3781 ggctcgcatt atgcgcggtt gcgccggctc gttgactcgc tataatgggc cggtgattat
     3841 ggaccgtcaa caggagaaca aaaattgaaa acgacattcg ccatgcttgt catttccctg
     3901 atgctcagcg ccgcgacggt gcgggcggaa gaagagaccg cacagacgct ggcagaggag
     3961 cttttggttc tgatggatgt gcagtccagc attcagaaat cctttgatgc cgtgaaacag
     4021 atgcaggcga cccagtttgc atccatggcg ggagaccgca aggagcaggc cctggccatg
     4081 cagcagaaga tgatggatct catagccggc gagatgagct gggacaagat caagaccgat
     4141 tatatcggca tttatgctga aaccttcact gcggatgaac tcaagggcat catagatttc
     4201 tacaagaccc cggtaggcag aaaattcatc gagaagcagc cggaaatcat ggaaaaatcc
     4261 atgcgcgtca ctcaaaaaca gatgatgacg ctgatgccga agatacaggc gctcgcactg
     4321 gaagccgcgg gcatgccgcc ggcgcagtaa tcagcggccg ccgataaggc tttcggcgag
     4381 ggctttctcc accgcatccg tcagctcccg gaaggtaaag ggcttctgga ggaacgtggt
     4441 ccgcttcatg gccgtggccc gatgaatatc cggctgattg gcggaaagcc cgctgaccag
     4501 aaccaccggc acatcagcgc cggcgcggcg cagccactgg cacatttcga ggccgttgaa
     4561 gtccggcatg ctgaagtcca tgataatcat gtccggcgga gtcgctcccc cggtccattt
     4621 gtcctgcaat tcatttgcac cggacagact caagaccgta tgcccctgcc ccgaaagaac
     4681 aaactcgcac atttttcgaa atgtttcatc gtcatccaca acccagatgg tggaaggctt
     4741 cagctgatcg ccgccgtccg ggctcgctgc acgggcgtcg gcctgagcga tggtcggaag
     4801 gatcaccttg acgcgcggct tcccgctgac tgcggccgga tcaatcaaga cagatccctc
     4861 caggtcggcg acaaggccaa acagactgga tatcttcagg ctggcatttt cgggcgctga
     4921 cgaatcgccg gtcaggggca tttcctccgc ggatttctcc ccttcctgct ccgcgacccc
     4981 aatttcgatg aagcggcgca gaacatttcc agtgcgcatc tccgtgttct gcgtgacgac
     5041 catgatgcat ccgggaccgt ccatggcatc gagcgaattg gttaccagat tgaaaatcac
     5101 ctgatgaaga gcgctgggcc gggcggatat ttcgtcattc ttagcgccca gattcatggt
     5161 aaagcgcacc cgtgaatgca cctgcgaatt caggagcgaa acaacccctt ccagaatctc
     5221 gtggaccccg catgtggcct gcacctcggc ctcgctcccg aggaaggcca gcatttcctc
     5281 ggtcaggcgg cggcccttct gggcggcctc ggaaatatgc tccagcgcat cggaagcttc
     5341 cggggggagg gacaagcggt tgagcatgaa agaggaatag cccagaatca cagaaagcag
     5401 actgttgaaa tgatgggcca cgccgcccgt taaagctccc agcgaccggg cccgctccga
     5461 actgatcaac tgctccgcca ggctgtcggc ccggcgggat tcatccagca gcacagacag
     5521 cagatcgcga agctggcgca gaaacgcggt gacctgctgt tcctcttcac gggtgtacac
     5581 gcgaaggctt tccacggccc gcttcagctc cttgtcagac agggttccgt gcgcttcaag
     5641 ttcagagagg agcttgccgc tgaccggctc ggtgatcatc cagccggtcc gcagacaaca
     5701 gcaaatcctg tcaccggcca gcacaggcag cgccgcctga taaaggccaa agcggctgga
     5761 cgcgttctgt tcctgccggg tctcaagaac ctttttgtac aaagtcgcct cctccctgag
     5821 gagattggcg tacagcttct ccgacgaact gatcaaggct tccacggctg tttcaaccgg
     5881 ggcgctgatc acgggacacc cggtcagaat ctgctgaacg ttttcatgcc gggcatcact
     5941 ttcttccaga acaggacaga gttccgccag cagggaatcc cgaagcagct tcagttcgga
     6001 aagagaaatg tttttcttca tatttcccta ccttcgcggc tgaacccggc gcttgatata
     6061 ttcatcacgg taggttttat cgcagcgcaa gcggctttcg tggctcagct ggttcaaggc
     6121 gatctcctcc gtgccgcccg ccacagccag caccaccaca tcgcttttca ggccgatcag
     6181 gcgcccgcga tgcacccggc ctgtttttcc gaccagagtg accgactcat ttttcggata
     6241 caacggggat tgtttatcca gttcgtcccg aaaccgctgc tctctctggg ccagaacccc
     6301 ggcttcttcc tccggcgatt gaacaacggc ctctgcaggc ggcgcggccg gcgcaggggc
     6361 ctccacagcc gccgcagcag gctcttcagc ggcagccgcc gcgggcggcg cggacacggc
     6421 cgggagcggc gccgcgctga ttcgcgcggt tgaggcttcc cactgctcac gcgccttctc
     6481 gagccgcttc tcgaagcggc tttcctgaac aatatagacc gccatgccgg aaataatcat
     6541 gagcagcaca ggcaccgcaa atcgcgacag cgccgtccct tggcggacaa tccgtgtatt
     6601 ttttttctgg cccgtctcca aattgtagcc gcactgagtg cagaccaggg cgtttcccgg
     6661 aacaggcgcg ccgcaggcag gacagtcgcg actgtcctct gtgaccccgg agctgtcctt
     6721 gatctgatag gattccagcc gtcccggatc atccggcatg tccaggaagg tttctttccc
     6781 gcaggccgga cagggcgcgg acgccccggc cttctccttg tccaccatga aaacctgccc
     6841 gcaaaacaaa cacttcactc tgctgtcact catcgctgct ccttaacgcc aactggttca
     6901 agtgcgatca tactaacagc cgggcggcac aattcaagcc gccgtttcaa gttctcctcc
     6961 gcttgtattg aaaaaactcg cccacgagtg tattctttcc gttgtatttg catggattca
     7021 gccatgctgg gaggtttttc tgccatgcag ctggacaaac tgacaatcaa atcgcaggaa
     7081 gctgtgcatc atgcgcgccg cctggccgaa caggctggac acggcgaaat cggcactccg
     7141 cacctggttg ccgccctgat tgaccaggag gaagggatcg tacgccccct gctccagcgt
     7201 ctgggcacgc cgcccgacgg gctgcgcgat caggtcgagc gcctgctgtc aaagcgtccc
     7261 cgggtcgagg gcgccgccgt tgatctgtat atctcccgcg atctgaagca ggtgctcgac
     7321 aacgcttttg ccctcgcgga gaagatgaag gacgagtatg tcagcacgga gcatcttttt
     7381 ctggcggcca tcgagaaggg cagcgatgaa ttccaggcca tcgcccgcca ggcaggactg
     7441 acgcccgatg ccgtgctgaa ggccctgcag gcggtccggg ggcgccagcg tgttacggat
     7501 ccggcccccg aggacaaata cgaggccatg aaaaaatacg gccatgacct cgggggccgg
     7561 atccgtaaca cgctggcgcc cccggaccgc ctgcagggcc
//
LOCUS       DAOQWR010000128         7572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_10115_length_7572_cov_5.291340, whole genome shotgun sequence.
ACCESSION   DAOQWR010000128 DAOQWR010000000
VERSION     DAOQWR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10115_length_7572_cov_5.291340"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKK36_09595"
     CDS             complement(<1..483)
                     /locus_tag="PKK36_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94843.1"
                     /translation="MRNVRRHAEKIFMVLFAAALLAAPAFAQDAVYSLNVVGFQKVAA
                     KPTALTLAGNPFESPDPELNSLISTQLTAGSSYGVADKILKWDSDTQSYVYYYLGNNA
                     RSGVVNKWVLSTDEGVPTTNAYLNNDDGFFIQTVAASTQQVVMVGDVVNDPEISKSIP
                     A"
     gene            699..2858
                     /locus_tag="PKK36_09600"
     CDS             699..2858
                     /locus_tag="PKK36_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR94844.1"
                     /translation="MPIKEIHDESAFLSMLEQEPIDFEPLRRWLEHEFRAGRRETADS
                     SAELMQVTLADQGLTDQALLILRMRAAHHPAPEAFRKVCEEEALAAVGPAPDDRALVY
                     SAGFDEEIPLAECFRRLELLRALKPGTLCYDATWGAGTVRGTDGFYKKVTIDFEKRRG
                     HELSYAYAGETLKLLDEDHLLARKMRDPQALEALIASDPAEIVRITLRSFGDMPVAML
                     QEVLIPRLMPESGWKAFWEAARRVLKKDPLFEIPAKRTDPLRILHKEKGFDGDWFSAL
                     AGERDMGALLERMDDYIAAGADAPSPEQLEALGERLAFVVKGAGLRHLDWRARAAMAA
                     DDLKVPASVVNAPELYETFFDAAHLHQALRGLSARDMKALLQHMLAFDAERMRDLLLS
                     VLSQLEMPALNEAMELLLANGAEEQCRAIFRELTVESQVADVEVLYWLLRHPEYMAKW
                     ELGTPGDHARWVVRALETQHSGEKLKTQNQLRKTFSKDAWLGPVLETMSPSERRELFL
                     RIKESPGWEMLDRRALLGLIIKRYPELEEEMRTSSESTSSDGAERGPLTSRRSYEARQ
                     RQFQKLVMEEIPAAAKDIATAREYGDLRENFEYKAAKDRQALLLQRKADLEAMLNKVR
                     PTTFDQVPTDRAGRGCMVTLRYADGREETFTILGAWDRDESLNIISSDSRMAVALRGH
                     RKGSRAVVPSEAGEVPCTVAEIAPLSDAVREWVNGAE"
     gene            2855..3355
                     /locus_tag="PKK36_09605"
     CDS             2855..3355
                     /locus_tag="PKK36_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNR94845.1"
                     /translation="MNLRALFRQFTQREAHPLVQFVKYGIAGGIATLVDMAIFYFLSW
                     QVLQALNSDDIVVRLLHLDVPAIDNALRSRNFVINSGIAFIFSNLTAYFINFHWVFHP
                     GRHSRHVEVALFYAVSLTSVFIGVGLGWGMIHFLRLSTTSSFIGKMFASLMINYVCRK
                     YIIFKR"
     gene            3405..6422
                     /locus_tag="PKK36_09610"
     CDS             3405..6422
                     /locus_tag="PKK36_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94846.1"
                     /translation="MSLARGRVPDLPLLPGKPAGGEKPLVHRGRPAGGEKPLVHRGKP
                     AGGEKIFSGSWLPAIFLILAAGCATPKTVEMPETDALPEEELAKIATARFLDNRPYEF
                     TWNNPDEAHAPLVDFEGLEGWTVVAPQGLKAKFERTYEKQLWGKYTAEFRCERAEDER
                     RFLIRPPSPVPIPEDFDSVSLWIHRVPEEGAAAATSRVDLVLRDARGMERVVPLAALD
                     WPQGWFLVQRRFGADALKGLRFPYEFAGLAVTCAGGREPLQIYFDSLSFFLAPRQPVA
                     APALETGIAADASVLPRPPATGIVHRTELVAPGVYRLACRKDGFEAEYRFEAALGLAG
                     LSLWRNGKPCGLLMAEAAVQGLPADSRVRVLRQEGAEVYAEYESGLSLRLALTNFSLS
                     LDARCPGGRAEGFSAGHLDGFDRVDLLPSPFLSFGLPVSPSVAMISGENRDVFFLSIW
                     PDWYRSAASEMTSPAAEGDSLAGGARYLPTTAGVRNDFKERIVLTVSDRIEDVLPRVR
                     HARAEDMKPLSDRLLLRVPGELDYRDVHQFLLGLCRYGMSNIIMATDRAVWTDGWGSG
                     GLRTKANPHRGGDERLKKYSDTAREAGALMALYTHSTELSPLNRNWNPAAVSAGPDGA
                     WLRLGASAYALKPSQAPETAGAVLRAAVSNYAPGAVVADGIADAPPWHHTDCDARAAG
                     AGRFTAAHAALATVLRGAPVPAVAPDETSPLYAGMADAFITGARAWNREPGDSYFPLF
                     KLMALQPLSLWFGPGMPFRANQPPDDRLLDEWMAAWLAHGQLGELPFGWNDPQRMARI
                     FYSMRALQPFYAGMAPRRIAWWDGRRLLTASEALARGMLEHSQMYFQYPSGFELWVNG
                     SPRKNWPVRIGGTEWTLPPAGWLASAPDFLSASVLVDGQRLDFVRTPDFVFYDGRGRE
                     QAFENHLSSASIVVRRADAPNKPGAIEVFDTSGGRRTGISADWFGGAEKVECRVQNED
                     GESWSEVSVRKEGDMYLFKGPSSGTRYALWPVR"
     gene            6666..>7572
                     /locus_tag="PKK36_09615"
     CDS             6666..>7572
                     /locus_tag="PKK36_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR94847.1"
                     /translation="MAKAVPGVVNIGTEKLVQVRYTDPFLSLRGDLLDRFFSDFFGPP
                     PLPGYRIGHSLGSGIILDPWGYILTNYHVLERASRIAVILSDETRYDARIVAGDEVAD
                     LALIKIDPLRPLTALEFADDDDLLLGETVIVLGNPFGLTHTVSVGVLSAMNRTAQYKG
                     RELYSDILQTDAAVNPGSSGGPLLNLDGELIGVNVAVYQEAQNIGFAIPVKRVRALLG
                     EWLSPERLKNIWLGFDLGDGLTISEVYEKTPAAAGGLLAGDVISAVDGRPVRTKYDLN
                     KALLRKELGDIARLTVARAEKPLDVE"
BASE COUNT         1338 a         2229 c         2549 g         1456 t
ORIGIN      
        1 agcggggatg gattttgaaa tttccgggtc attgaccaca tcgccaacca ttacaacctg
       61 ctgggttgag gccgcgacag tctggatgaa aaacccgtca tcgttgttca aataagcgtt
      121 tgtggtgggt acgccctcat ctgttgaaag gacccattta ttaaccacgc ccgaacgggc
      181 attgttgccg agataataat agacgtatga ttgtgtatcg gaatcccatt taagaatttt
      241 gtctgcaaca ccgtagctcg atcctgccgt cagctgcgtg gagatgaggg aattgagttc
      301 ggggtcggga gattcgaagg ggttgcctgc aagggtcagg gctgtgggtt tggcggccac
      361 tttctggaag ccgacgacgt tgagggagta gacggcgtcc tgtgcaaagg ctggcgcagc
      421 aagaagggct gccgcaaaca ggaccatgaa aatcttttcc gcatgacgac gtacattgcg
      481 cataattacc tcacctatga tttatccaaa tattacgcag acccgggttc ctgtaaagtc
      541 ctttttggaa aattttagac ggttattttt ttcttcattc cgggccatca acaccccggc
      601 cagcggcttc cttgaaaaaa atgcggccgg ggattgccaa accccggttt tacgggcatt
      661 atgccgcttc gaatcacgtt acggatggca ggagttctat gcctattaag gaaatacacg
      721 atgaaagcgc gtttctctcc atgctggagc aggagccgat tgattttgaa cccctccgcc
      781 gctggctgga gcacgagttc cgggcgggcc gccgcgagac ggccgacagc agcgccgagc
      841 tgatgcaggt gacgctggcc gatcaggggc tgacggacca ggccctcctt atattaagga
      901 tgcgcgcggc ccatcatccc gcccccgaag cattccggaa ggtgtgcgag gaggaggccc
      961 tggccgccgt gggccccgcg cccgacgacc gcgcgctggt gtactcggcc ggattcgatg
     1021 aggagatccc gctggccgag tgcttccgca ggctggaact gctgcgcgcg ctgaagcccg
     1081 gcaccctgtg ctatgacgcg acctggggcg ccggcacggt gcgcggcacg gacggattct
     1141 acaagaaggt gacgattgat ttcgagaagc ggcgcgggca cgagctttcg tatgcctatg
     1201 ccggcgagac cctgaaactg ctggacgaag atcacctgct ggcgcggaag atgcgcgatc
     1261 cgcaggcgct ggaggcgctg atcgcctctg atcccgcgga gatcgtgcgc atcacgctgc
     1321 gcagtttcgg cgacatgccg gtggccatgc tgcaggaggt cctgattccg cggctgatgc
     1381 ccgagtccgg ctggaaggcc ttctgggagg ccgcccgcag ggtcctgaag aaagatccgc
     1441 tgtttgaaat tccggcgaag cggaccgatc cgctgcgcat tctccacaag gaaaagggtt
     1501 ttgacgggga ctggttttcg gcgctggccg gggagcggga catgggcgcg ctgctggagc
     1561 ggatggacga ttacatcgcc gccggcgctg atgctccgtc gccggagcag cttgaggcgc
     1621 tgggggagcg gctggccttt gtggtgaagg gcgccggcct gcggcatctg gactggcgcg
     1681 cgcgcgcggc gatggctgcg gacgacctga aggtgcccgc ttccgttgtg aatgccccgg
     1741 aactgtatga gacgttcttt gatgccgcgc atctgcacca ggcgctgcgc gggctttccg
     1801 cgcgcgacat gaaggcgctt ctgcagcaca tgctggcctt tgacgccgag cggatgcgcg
     1861 acctgctgct gtcggttctc tcgcagcttg agatgccggc gctgaacgag gcgatggaac
     1921 tcctgctggc gaacggagcc gaagagcagt gccgggcgat cttccgcgag ctgaccgttg
     1981 agagccaggt ggcggatgtg gaggtgctct actggctgct gcgccacccg gaatacatgg
     2041 cgaaatggga gctgggcacg ccgggcgatc atgcgcgctg ggtggtccgc gcgctggaaa
     2101 cacagcacag cggcgaaaag ctgaagacgc agaaccagct tcggaaaact ttttcaaagg
     2161 acgcctggct ggggccggtg ctggagacga tgagcccgtc cgagcgccgc gagttgttcc
     2221 tgcggatcaa ggaaagtccc ggctgggaaa tgctggaccg ccgcgccctg ctggggctga
     2281 tcatcaagcg ctatcccgag cttgaggagg aaatgcggac atcgtccgag agcacgtcct
     2341 ccgacggcgc cgagcggggc ccgctgacat cgcgccgcag ctatgaggcg cgccagcgcc
     2401 agtttcagaa gctggtcatg gaggagattc cggcggcggc gaaagacatc gcgaccgcgc
     2461 gcgagtacgg cgacctgcgc gagaatttcg aatacaaggc ggcgaaggac cggcaggcgc
     2521 tcctgctgca gcgcaaggcg gatctcgagg ccatgctgaa caaggtgcgg cccacgacgt
     2581 ttgaccaggt cccgaccgac cgcgcgggac gcggatgcat ggtgacgctc cgctacgccg
     2641 acggacggga ggaaaccttt acgattctcg gcgcgtggga ccgcgacgaa tccctcaaca
     2701 tcatatcgtc cgacagccgc atggccgtgg cgcttcgcgg acaccgcaag ggctcgcgcg
     2761 cggtggtgcc gtcggaggct ggcgaggtgc cctgcacggt ggcggagatc gccccgcttt
     2821 ctgacgccgt cagggaatgg gtgaacggag ccgaatgaat ctcagggcgc tattcaggca
     2881 gttcacgcag cgcgaggcgc atccgctggt gcagtttgtg aaatacggca tcgccggcgg
     2941 cattgcgacg ctggtggaca tggccatttt ctattttctt tcgtggcagg ttctgcaggc
     3001 gctcaacagc gatgacattg tggtgcgcct gcttcacctc gacgtgcccg cgatagacaa
     3061 cgcgctgcgg tcgcgcaatt ttgtgatcaa cagcggcatt gctttcatct tttcgaacct
     3121 gaccgcctat ttcatcaatt tccactgggt gtttcatccg ggccggcaca gccggcatgt
     3181 ggaggtggcg ctgttttatg cggtgtcgct gaccagcgtt ttcatcggcg ttgggcttgg
     3241 ctggggcatg attcattttc tgcggctcag cacgacgtcg tcgttcattg gcaaaatgtt
     3301 cgcctcgctg atgatcaatt atgtgtgcag gaaatacatc atattcaagc gataggggcg
     3361 gggaatcgaa agccccccgc gaccttgtgt cgcgggtgaa taaggtgagc cttgctcgcg
     3421 ggcgggtgcc tgaccttccg ctcctgccgg gcaagccggc agggggcgag aagcctctgg
     3481 ttcatagggg caggccggca gggggcgaga agcctctggt tcataggggc aagccggcag
     3541 ggggcgagaa gatattttcc ggttcgtggc tgccggcgat ttttcttatc ctggccgccg
     3601 gatgcgcgac gccgaagacg gtggagatgc ccgagaccga cgcgcttccc gaagaggagc
     3661 tggccaagat cgccacagcc cgctttctgg acaaccgtcc gtacgagttc acctggaaca
     3721 atccggacga ggcgcatgcg ccgctggtgg atttcgaggg gctggagggc tggacggtgg
     3781 tggcgccgca gggcctgaag gcgaagttcg agcggaccta tgagaagcag ctctggggga
     3841 aatacacggc ggagttccgc tgcgagcgcg ccgaggacga gcgccggttc ctgatccgtc
     3901 cgccgtcgcc ggtgccgatc ccggaggatt ttgattcggt ttcgctctgg attcaccggg
     3961 tgcccgagga gggcgcggcc gccgcgactt cccgcgtgga tctggtgctg cgcgatgccc
     4021 gggggatgga gcgcgtggtc ccgctggcgg cgctggactg gccgcagggc tggtttctgg
     4081 tgcagcgcag gttcggcgcc gacgcgctca agggactgcg cttcccatac gagtttgccg
     4141 ggctggccgt cacctgcgcg ggcggccgcg aaccgctgca gatctatttc gattcgctgt
     4201 cttttttcct cgcgccccgc cagccggtgg ccgcccccgc gctggaaacg ggcatcgcgg
     4261 cggatgcttc ggtgctcccg cgtccgcccg ccaccgggat tgtgcatcgc acggaactcg
     4321 tcgcgcccgg ggtgtaccgg ctggcctgcc gaaaggacgg gtttgaggcc gaataccgct
     4381 ttgaagccgc gctgggcctt gcggggctca gcctgtggcg caacgggaag ccctgcggcc
     4441 tgctgatggc ggaggccgct gttcagggcc tgcctgctga cagccgcgtg cgcgtgctcc
     4501 ggcaggaggg cgctgaggtc tatgccgagt atgaaagcgg gctctcgctg cggctggccc
     4561 tgaccaattt cagcctgtcc ttggacgcgc gctgccccgg cggccgcgcc gagggttttt
     4621 ccgccggaca tcttgacgga ttcgatcgcg tggacctgct gccgtccccg ttcctttcgt
     4681 tcggcctgcc cgtttcgccc tccgtggcga tgatcagcgg cgaaaaccgc gatgtctttt
     4741 tcctgagcat ctggccggac tggtaccggt cggcggcttc ggaaatgact tcgcccgcgg
     4801 ccgaaggcga cagcctggcg ggcggcgccc gctatctgcc gaccaccgcc ggcgtgcgga
     4861 atgattttaa ggaaagaatt gtcctgacgg tttcggaccg cattgaagac gtgctcccgc
     4921 gggtgcggca cgcacgcgcg gaggacatga agccgctttc cgaccgcctg ctgctgcggg
     4981 ttccgggcga gctggattat cgggatgtgc atcagttcct tctgggtctt tgccgctacg
     5041 gcatgagcaa catcatcatg gccaccgacc gcgcggtctg gaccgacggc tgggggagcg
     5101 gcggcctgcg cacgaaggcc aatccgcacc ggggcggcga cgagaggctg aagaaatatt
     5161 ccgacaccgc gcgcgaagcc ggcgcgctga tggcgctcta cacgcacagc acggaacttt
     5221 cgccgctcaa ccggaactgg aatccggcgg cggtcagcgc cggtccggac ggcgcatggc
     5281 tgcggctggg cgcatccgcc tatgcgctga agccctcgca ggcgcccgaa acggccggag
     5341 ccgtcctgcg cgcggcggtt tccaactacg cgccgggcgc ggtggtcgcg gacggcatcg
     5401 cggatgcgcc gccgtggcat catacggact gcgacgcgcg cgcggccggc gcgggaagat
     5461 tcaccgcggc ccacgccgcg ctggcgacgg tgctgcgcgg cgcgcccgtg ccggctgtcg
     5521 cgccggatga aacctcgccg ctctatgcgg ggatggccga tgccttcatc accggcgcgc
     5581 gcgcatggaa ccgcgagccc ggcgacagtt atttcccgct gttcaagctg atggcgctcc
     5641 agccgctcag tctgtggttc ggccccggca tgcccttccg cgcgaaccag ccgcctgacg
     5701 accgcctgct tgacgaatgg atggcggcat ggctggccca tggacagctt ggagaactgc
     5761 cgttcggctg gaacgatccg cagcggatgg cgaggatttt ttattccatg agggctcttc
     5821 agccgttcta tgcgggcatg gctccgcgca ggatcgcgtg gtgggacggc cgcaggctcc
     5881 tgaccgcttc cgaggccctc gcgcgcggca tgctggagca ctcgcagatg tatttccagt
     5941 atccctccgg tttcgaactg tgggtgaacg gatcgccccg gaaaaactgg ccggtcagga
     6001 tcggcggaac ggaatggacg cttccgcctg ccggatggct ggcctccgcg cccgattttc
     6061 tttccgcctc cgtgctggtg gacgggcagc gcctggattt tgtccggacc ccggattttg
     6121 ttttttatga cggccgcggc cgcgaacagg cttttgagaa ccatttgtcg tccgcgagca
     6181 ttgtcgtgcg ccgcgcggat gccccgaaca agcccggcgc catcgaggtg tttgacacct
     6241 ccggcgggcg cagaacgggc atcagcgccg actggttcgg cggcgcggag aaagtggaat
     6301 gccgcgtgca gaacgaggac ggcgaaagct ggtcggaagt gtccgtgcgc aaagaggggg
     6361 acatgtatct ttttaaggga ccctccagcg gtacgcggta cgcgctctgg cctgtccgct
     6421 gacgggcttg accagaggag cagaaaattg ctaaaaaatc aagaaaaaac gctcaaaaac
     6481 ggtcaaaaat gatcaaaaaa cgctcaaaaa cggtcaaaaa tgatcaaaaa acgcaaaaaa
     6541 agtcgttttt ttgccaaaaa tgcgtgaaaa ttccgatttt tggccttttt ttcatttttt
     6601 tagcgatttt ttccgggcat ggtcttgaac cggacagtcc gcgcttttcg ccggtggtga
     6661 aggccgtggc caaggcggtt ccgggcgtgg tgaatatcgg aactgagaag ctggtgcagg
     6721 tgcgctacac cgatcctttt ttaagtctgc gaggggacct gctggaccgg tttttcagcg
     6781 attttttcgg gccgccgccg cttccgggct accgcattgg tcattcgctc ggttcaggca
     6841 tcattctgga cccctggggc tatattctca ccaattacca tgtcctggaa cgggcttcgc
     6901 gcattgcggt gatcctgtcc gacgaaaccc gctacgacgc gcgcattgtc gcgggcgacg
     6961 aggtcgccga cctcgcgctg atcaagattg atcccctgcg gccgctcacc gcgctggaat
     7021 ttgccgatga tgacgacctg ctgctgggcg agaccgtgat tgtgctgggc aatccgttcg
     7081 gcctcacgca tacggtctca gtgggggtgc tttcggcgat gaaccgcacc gcgcagtaca
     7141 agggcaggga gctgtacagc gatatcctgc agaccgatgc ggccgtgaat cccggaagtt
     7201 ccggagggcc cctgcttaac ctcgacggtg aactcatcgg cgtgaacgtg gccgtctatc
     7261 aggaggcgca gaacatcggc tttgccattc ccgtcaagcg ggtccgcgcg ctgctgggcg
     7321 agtggctctc gcccgagcgt ctcaagaaca tctggctggg ctttgatctg ggggacggac
     7381 tgacgatctc agaggtgtac gaaaaaactc cggcggccgc aggaggactg ctcgcgggcg
     7441 acgtgatctc cgccgtggac ggccgccccg tgcgcacgaa atacgatctg aacaaggcgc
     7501 tcctgcggaa ggaactgggc gacatcgcgc gcctgaccgt cgcgcgcgcg gagaaaccgc
     7561 tggatgtgga gc
//
LOCUS       DAOQWR010000129         7100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_10958_length_7100_cov_5.157133, whole genome shotgun sequence.
ACCESSION   DAOQWR010000129 DAOQWR010000000
VERSION     DAOQWR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_7100_cov_5.157133"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKK36_09620"
     CDS             <1..708
                     /locus_tag="PKK36_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNR94848.1"
                     /translation="VAAEGRKILEGMIAWLRENGGTPVEIDTDGIYFVPPEVKSKAEM
                     ERFRTAFQKSLPRGISLEFDGEYKSMFSYKMKNYALLAEDGEMVIKGAALKSRGLEPF
                     QRSFLEEFIRLRLEGRDGDIPELKRRYEQDILHGRWPVRMLAKTETLQESPAGYAAKV
                     DAKGRGRNAAYELALKSGREYQAGDQISYYVTGSRKSVQVFAAAKLVSEWNPESRDEN
                     IPYYLAKLGALCKKFGI"
     gene            714..1157
                     /gene="gspG"
                     /locus_tag="PKK36_09625"
     CDS             714..1157
                     /gene="gspG"
                     /locus_tag="PKK36_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNR94849.1"
                     /translation="MNKRKHLSAGFTLMEILVVLIIITILAGLVGVNVLRKPGEARVS
                     AARMQIRNLQTAVKLYHAEQGHLPSQQQGLEALVRKPSMEPVPKNYPAGGYLDSAELP
                     RDPWGNDYIYLTPGRHGEPFEIISYGSDGQPGGEDDAGDISSSDA"
     gene            1150..1470
                     /locus_tag="PKK36_09630"
     CDS             1150..1470
                     /locus_tag="PKK36_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR94850.1"
                     /translation="MRDGFTLVETLIALVMASILLSVSASALMKILRAEVTASKMQQA
                     SLAMQTIGCRAALGDEEPADLPRITLRKHALSVAGAEWAVWDITCTSAPSFSATLEYP
                     VPAR"
     gene            1585..2454
                     /gene="ilvE"
                     /locus_tag="PKK36_09635"
     CDS             1585..2454
                     /gene="ilvE"
                     /locus_tag="PKK36_09635"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HNR94851.1"
                     /translation="MKIYMSGKLVNEKDAKVSVFDHGLLYGDGVFEGIRAYNGRVFML
                     DEHIKRLYDSAKAIALDIPMTPESMAKAVVKTCRANDIRDGYIRLVVTRGVGTLGLNP
                     YLCKKAEVIIIAAAIQLYPKKLYDDGLAVITAGTTRNLQEALNPRIKSLNYLNNIMAK
                     IEAINSGVMECLMLNPQGYVAEASGDNIFVVRGNDLITPPSWCGALEGVTRNVVMSIA
                     RDAGINVRESVLGRYDIYTADEVFLTGTAAEVIAVVNADRRKIGNGKPGPVTRRLTRL
                     FHEFARANGTEIK"
     gene            2488..2853
                     /locus_tag="PKK36_09640"
     CDS             2488..2853
                     /locus_tag="PKK36_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR94852.1"
                     /translation="MGETAKTFEDLIVWQKAHQFVLSVYRITQKFPRSEVYGLSSQFR
                     RAAVSVAANIAEGFRKRGSADKMRFYNIAQGSLEECRYYLILTKDLEYGDVSETGLLL
                     TEVSKLLDAYSRKILNSDS"
     gene            2917..3411
                     /locus_tag="PKK36_09645"
     CDS             2917..3411
                     /locus_tag="PKK36_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNR94853.1"
                     /translation="MLCDRCKENDATVHLTQVVNGVVEKLHLCESCAAEAGFDLQGPV
                     SITDILLGMGAKQDEAAEHIAEKICPDCHMTMSDFKKCGRLGCARCYEIYRDELGLLL
                     KAVHRSEQHTGKIPARDRERVHHSQEAAALQKELADAVAREDYEEAARLRDRLRMLAD
                     HETR"
     gene            3398..4474
                     /locus_tag="PKK36_09650"
     CDS             3398..4474
                     /locus_tag="PKK36_09650"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HNR94854.1"
                     /translation="MKLDKFLKNQGAWMGGGGDSDIVISSRVRLARNLRDETFPGRAS
                     DEQRLKIWGRLKPVLASLAAMDPSVSLYMGDLSDLDRMILFERHLISRELTVPRAGQG
                     VVIRQDERAAIMVNEEDHLRLQALRPGLMLQNAWECVDEMDSRIEEMVPYAFSEKLGY
                     LTSCPTNVGTGMRASVMLHLPGLVFMEEVGPVIKGLNKIGLTVRGLWGEGTEAVGNMF
                     QVSNQITLGDCEKEIVSHLQTIVEEVVEHEQNARLRLMESREHVVLDYVGRALGILSN
                     AHILASKEALGLLSGLRLGIEMGILKDFNRQTVDDLLVSAQPAHLQVIEKAELAPEER
                     DRARAELIRKRLARRRNRKRSKNE"
     gene            4467..6944
                     /locus_tag="PKK36_09655"
     CDS             4467..6944
                     /locus_tag="PKK36_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNR94855.1"
                     /translation="MNNFTPRAQQVLHLARKEADRFNHAYVGTEHILLGLIALGHGVA
                     INVFNAMGIDLETVRMEVEKAVGVGPETKTVGNVPFTPRVKKVLALASSEARALQHSY
                     VGTEHILLGLLREGEGVAARILQNLNVDLERTRVEIMKELNPNYDPTAQGPEGPLEAP
                     TGPGTAGKSAQEGKTPALNTFGRDLTELAKKNGLDPVIGRANEIERVIQILCRRTKNN
                     PVLLGEAGVGKTAIVEGLAQAIVAGEVPEILYGKKVIALDLALMVAGTKYRGQFEERI
                     KAVMDEIKRSKDVILFIDELHGIVGAGSAEGAMDASNIIKPSLARGELQCVGATTLAE
                     YRKFIEKDAALERRFQSVIVREPTVDETIEILKGLRSRYEEHHHARISDEAIKAAATL
                     SARYISDRFLPDKAIDVIDEAGSRSRISSMMRPPNVKDAEDKIAEIRKQKEDAIRAQK
                     FEAAAAFRDQERDARKEVDRLLEEWRKARDEKIVTITAEDVMHILSKWTGVPLVRMED
                     HEMNRLLGMEKELEKSVIGQHEAVSAISRALRRSRADLKDPRRPIGSFIFLGPTGVGK
                     TLLAKVLAEFMFDDPDALIQIDMSEYMEKFASSRLVGSPPGYVGYGEGGQLTEQVRRR
                     PYSVVLFDEAEKAHPDVMHMLLQILEEGKLTDSLGRTVDFRNTVVILTSNLGADFIQK
                     GAGLGFGETTAGSDFKKLEAKMTEESKKVFKPELLNRIDKIIVFHQLLRDDVMKILEV
                     ELVKVLKRLDAKNIRVQMSDAAKDFLVEKGYDPAYGARPLRRAIEKYIEDPLAESILR
                     GEIHPDESVEVVVRGDALAFQQLSAAT"
BASE COUNT         1600 a         1967 c         2203 g         1330 t
ORIGIN      
        1 gtggccgccg aaggccgtaa aatcctggag ggcatgattg catggcttcg ggaaaatggc
       61 ggaacccccg tcgagattga caccgacggc atctatttcg ttccgccgga ggtcaagtcg
      121 aaggctgaaa tggagcggtt ccgaaccgct ttccagaagt cgctccccag gggcatatcg
      181 ctggaattcg acggcgaata caagtccatg ttcagctaca agatgaagaa ctacgcgctg
      241 ctcgccgaag acggggagat ggtcataaag ggcgctgctc tgaaatcgcg cggtctggag
      301 ccgttccagc ggagtttcct tgaggagttc atccggctcc ggctggaggg ccgcgacggg
      361 gatattccgg aactgaaacg ccgctatgaa caggatatcc tgcacggccg ctggccggtc
      421 agaatgctgg ccaagaccga aacgcttcag gaatctcccg ccggctatgc cgcaaaggtt
      481 gatgcaaagg gccggggacg caacgccgcc tacgaacttg cgctgaaatc gggccgggaa
      541 taccaggcgg gggaccagat ttcatattat gtcaccggga gcagaaaatc agtgcaggtg
      601 tttgccgccg caaagctggt ttcagaatgg aatccggaga gccgcgatga aaatattcct
      661 tactatcttg cgaaactcgg cgcgctgtgc aaaaaattcg ggatatgaaa gtcatgaata
      721 aaagaaaaca tttgtcggcc ggcttcaccc tgatggaaat tctcgtggtg ctgatcatca
      781 tcaccattct ggccgggctg gtcggggtga atgtcctgcg aaagccgggg gaggcacgcg
      841 tgtccgccgc ccggatgcag atcaggaatc tgcagacggc cgtgaaactg taccatgccg
      901 aacaggggca tcttccttca cagcagcagg gcctcgaggc actggtgcgc aaaccgtcca
      961 tggagccggt gccgaagaat tatccggcgg gcggatatct cgacagcgcc gaactgccca
     1021 gagatccgtg ggggaacgac tacatctatc tgacgcccgg ccgtcacggc gagccgtttg
     1081 aaatcatcag ctacggaagc gacggccagc ccggaggaga agacgacgca ggcgacatct
     1141 ccagttcaga tgcgtgacgg attcacgctg gtcgaaacgc tgattgccct tgtgatggct
     1201 tccatcctgc tttcggtctc ggcttccgcc ctgatgaaaa tcctgcgggc ggaggtcacc
     1261 gcatcaaaaa tgcagcaggc ttcgctggcc atgcagacga taggatgccg cgcggcgctg
     1321 ggggatgaag agcctgccga cctgccgcgg attaccctcc gaaagcatgc gttgagcgtg
     1381 gccggcgcgg aatgggccgt gtgggatatc acctgcacct ccgcgccctc cttctccgcg
     1441 acgctggagt atccggttcc ggcccgctag gcgcctccgg ttcttcttcg atttttcgct
     1501 gttaagaatt gacgccggtt ggactggaat ttacccggcg cttctgctat cctacgaggc
     1561 aggttttatt tacatggagt gactatgaag atttacatga gcggaaaact ggtgaacgaa
     1621 aaagacgcaa aggtttcggt ctttgatcac ggtctgctgt acggcgacgg ggtgtttgaa
     1681 ggcatacgcg cctataacgg ccgcgtgttc atgctggatg aacacatcaa gcggctgtat
     1741 gactccgcga aggcgattgc gctggatatc ccgatgactc ccgaatccat ggcaaaagcc
     1801 gtggtgaaaa cctgcagggc caacgacatc cgcgacggct atatccggct ggtggtcacg
     1861 cgcggtgtgg gcacgctggg actgaacccg tatctctgca aaaaggccga agtgatcatc
     1921 attgccgcgg cgattcagct ctatcccaag aagctgtatg acgacgggct ggccgtgatc
     1981 acggccggaa cgacgcgcaa cctgcaggag gcgctgaatc cgaggatcaa gagcctgaac
     2041 tacctgaaca acatcatggc caagatcgag gcgatcaact ccggggtgat ggagtgtctg
     2101 atgctcaacc cgcagggcta tgtggccgag gcgtccggcg acaacatttt cgttgttcgc
     2161 ggcaatgacc tcatcacgcc gccatcctgg tgcggcgcgc tggagggagt tacccgcaac
     2221 gtggtgatga gcattgcccg cgacgcagga atcaatgtgc gcgagagcgt cctgggccgc
     2281 tacgatattt acacggccga tgaagtcttc ctgacgggaa ccgctgcgga agtcattgcg
     2341 gtggtcaatg ccgatcgccg gaaaatcgga aacgggaaac ccggaccggt gacccgcagg
     2401 ctgaccagac tgttccacga attcgcccgt gcgaacggga cggaaatcaa gtagtcagta
     2461 gtcagaattc aggagtcagg agacagaatg ggagagactg ccaaaacgtt tgaagatttg
     2521 attgtttggc agaaggctca ccagtttgtt ctttcagtct accggataac acagaagttt
     2581 ccgcgctcgg aggtctacgg tttgtcgtca caattcaggc gcgcggccgt gtctgttgcc
     2641 gcaaatattg cagagggatt tcgtaaacgt ggttcggcgg ataagatgcg attttacaat
     2701 atcgcccagg gttccttgga ggaatgccgg tactacttga ttctgacaaa ggatcttgaa
     2761 tatggagatg tgtccgaaac gggccttcta ctgacagaag tcagcaagtt gctggatgca
     2821 tattcgcgga aaattctgaa ttctgactcc tgacttctgt cttctgtctt ctgatttcgg
     2881 ttggagtata ttggtaaatg gaaggacact gacatcatgc tgtgcgatcg ctgcaaggaa
     2941 aacgacgcga cggttcatct tacgcaggtg gttaacggcg tggtggagaa gcttcacctg
     3001 tgcgaaagct gcgccgccga ggccggcttt gatttgcagg gccccgtgtc catcaccgat
     3061 attctgctgg ggatgggcgc gaagcaggat gaggcggccg agcacatcgc ggagaagata
     3121 tgcccggact gtcacatgac catgagcgat ttcaaaaaat gcggccggct gggctgtgcc
     3181 cgatgctatg agatttaccg cgatgagctc ggcctgcttc tgaaggcggt gcaccgcagc
     3241 gaacagcaca ccgggaaaat cccggcccgc gaccgcgagc gtgtgcatca ttcgcaggag
     3301 gccgcggcgc ttcagaagga actggccgat gcggttgccc gggaggatta tgaagaggct
     3361 gcgcggctgc gcgaccgcct caggatgctt gctgatcatg aaactcgata aattcctgaa
     3421 aaaccagggc gcgtggatgg gcgggggcgg cgactccgat attgtgattt ccagtcgcgt
     3481 gcgactcgcc cgcaatctcc gcgatgaaac ttttcccggc cgggcatccg atgagcagcg
     3541 cctcaaaata tggggcaggc tcaaacccgt gctggcgagc ctggccgcga tggatccgtc
     3601 cgtgtcttta tatatgggcg atctcagcga tctggaccgg atgattctct tcgaacggca
     3661 tctgatcagc cgcgaactca cggtccctcg cgcggggcag ggggtggtca tccgtcagga
     3721 tgagcgcgcc gcaatcatgg tcaatgagga ggaccacctg cgccttcagg ccctgcggcc
     3781 cggactgatg ctccagaacg cgtgggaatg cgtggatgaa atggacagcc gcattgagga
     3841 gatggtgccc tatgcctttt ccgagaagct gggctatctg acctcgtgcc cgaccaatgt
     3901 cggcaccggc atgcgcgcca gcgtgatgct tcatctgccg ggactggtgt tcatggaaga
     3961 ggtggggcct gtcatcaagg ggctgaacaa gatcggcctg accgtgcgcg gcctctgggg
     4021 cgaagggacc gaggctgtgg gcaacatgtt ccaggtgtcc aaccagatca cgcttggcga
     4081 ctgtgaaaag gagattgtga gccacctgca gaccattgtt gaggaagtgg ttgaacacga
     4141 acaaaatgca aggctccggc tcatggaaag ccgggagcat gtggtgcttg actatgtcgg
     4201 ccgcgccctc ggaattctga gcaatgccca tattctggcc tccaaggagg ctttgggcct
     4261 gctttccggc ttgcgcctgg gcatagagat gggtatattg aaggacttta ataggcaaac
     4321 ggttgacgat ttgctggttt cggctcaacc tgctcatttg caggtgattg agaaggccga
     4381 actggccccg gaagaacggg accgggcccg tgcggagctg atcaggaagc gtttggcacg
     4441 gcgcagaaac agaaaacgat caaaaaatga ataattttac gccaagagcc cagcaggtgc
     4501 tgcatctggc ccgcaaggaa gcggaccggt tcaatcacgc ctacgtgggc acggaacata
     4561 ttctgctggg gctgattgcc ctcggccacg gagtggccat caacgtgttc aatgccatgg
     4621 gcattgatct cgaaaccgtc cgcatggagg ttgaaaaggc cgtgggcgtg gggcccgaga
     4681 ccaaaaccgt gggcaatgtg cctttcacgc cgcgtgtaaa aaaagtgctg gctctggcca
     4741 gcagcgaagc gcgcgcgctg cagcattcct atgtcggcac ggagcacatt ctgctgggcc
     4801 ttctccgtga aggggaaggc gtggccgccc ggatcctgca gaacctgaac gtggatcttg
     4861 agcgcacgcg cgtcgagatc atgaaggagc tgaacccgaa ctatgacccg accgcacagg
     4921 ggccggaagg tcctctggag gctcccacgg ggccgggaac ggccggaaag tcagcgcagg
     4981 agggcaaaac cccggcgctg aacaccttcg ggcgagacct gacggaactg gcgaagaaaa
     5041 acggacttga tccggtgatc ggccgcgcca atgaaatcga gcgcgtgatc cagattctct
     5101 gccgccgcac caagaacaac ccggtgctgc tgggcgaagc gggtgttggc aaaaccgcta
     5161 tcgtcgaggg cctggcccag gccattgtgg ccggcgaagt gcccgaaatc ctctacggca
     5221 agaaagtcat cgccctcgac ctcgcgctga tggtcgccgg caccaaatac cgcggccagt
     5281 tcgaggagcg catcaaggcc gtcatggacg aaatcaagcg cagcaaggac gtgatcctgt
     5341 tcattgacga gctgcatggc atcgtcggcg cgggatcggc ggaaggcgcc atggacgcct
     5401 ccaacatcat caagccttcg ctggcgcgcg gcgaactgca gtgcgtgggc gccaccacgc
     5461 tggcggaata ccgcaagttc atcgagaaag acgccgcgct ggagcgccgt ttccagtcgg
     5521 tcatcgtccg ggagccgacg gtggatgaaa cgatagaaat cctgaagggc ctgcgctcgc
     5581 gctacgagga gcaccaccac gcgcgcatct cggacgaggc catcaaggcg gccgccacgc
     5641 tgtcagcccg ctacatatcg gaccggttcc tgccggacaa ggcgattgac gtgatagacg
     5701 aagccgggtc ccgctcgcgc atttcctcca tgatgcgtcc gcccaatgtg aaggatgcgg
     5761 aggacaaaat cgcggaaatc cgcaagcaga aggaagacgc catccgcgcc cagaaattcg
     5821 aggcggcggc cgcattccgc gaccaggagc gggacgcccg caaggaagtg gaccgcctgc
     5881 tggaagaatg gcgcaaggcc cgcgatgaga aaatcgtcac gatcacagcc gaagacgtga
     5941 tgcacattct ctccaaatgg accggagttc ctctggtccg catggaagac catgaaatga
     6001 accgcctgct ggggatggaa aaggaacttg agaaatccgt gatcggccag cacgaagccg
     6061 tctcggccat ttcccgcgcg ctgcgccgct cgcgcgcgga cctgaaggat ccccgtcggc
     6121 ccatcggctc gttcatcttc cttggcccga cgggcgtggg caaaacactc ctggccaagg
     6181 tgctcgcgga gttcatgttc gatgatcccg acgcgctgat ccagatcgac atgtcggaat
     6241 acatggaaaa attcgcatcg tcccgcctgg tgggctcacc gcccggctac gtgggctatg
     6301 gcgagggcgg tcagctcacc gagcaggtcc gccgccggcc ctattccgtg gtcctcttcg
     6361 acgaggccga aaaggcccat cccgatgtca tgcacatgct cctgcagatc ctggaggagg
     6421 ggaagctcac cgacagcctt gggcgcaccg tggacttccg caacaccgtc gtaatcctga
     6481 cctccaacct cggagccgac ttcatccaga agggcgcggg gcttggattt ggcgagacca
     6541 ccgccggctc cgacttcaaa aagctcgaag caaaaatgac ggaagaatcc aaaaaagtct
     6601 tcaagccgga actgctcaac cgcatagaca aaatcatcgt cttccaccag cttctgcgcg
     6661 acgacgtcat gaaaatcctt gaagttgagc tcgtaaaggt gctcaagcgc ctggacgcga
     6721 aaaacatcag ggtgcagatg tccgatgcgg caaaggattt ccttgtcgaa aaaggctatg
     6781 acccggccta cggcgcgcgg ccgctgcggc gcgcgattga gaaatacatc gaggacccgc
     6841 tggccgaaag catcctgcgc ggcgaaatcc atcccgatga aagcgtcgaa gtggtggtcc
     6901 gcggcgacgc gctggcgttt cagcaacttt ccgccgcaac ctgaaaaggg cgggcatccc
     6961 ccgcgccagg ctcccgcgac cttgtgtcgc gagtgaatca ggtccgctga aaagagcctc
     7021 aaaaagtcca aaaaaacgca agaaatgggg ttcatccgac aagttcctgt ctggagaggt
     7081 gcagattgaa aagtttgagg
//
LOCUS       DAOQWR010000130         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_11047_length_7055_cov_6.003714, whole genome shotgun sequence.
ACCESSION   DAOQWR010000130 DAOQWR010000000
VERSION     DAOQWR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11047_length_7055_cov_6.003714"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKK36_09660"
     CDS             complement(<1..759)
                     /locus_tag="PKK36_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNR94856.1"
                     /translation="MAIKVGINGFGRIGRLVFRAAVKNPDVEVVGVNDPFISGDYMVY
                     MLKYDTVHGRFKGTAEFKDGHLVVNGKKTAVYAQKDPAMIPWKECGAEYVLESTGVFT
                     VLEKAEAHIKAGAKRVMISAPSKDAPMFVMGVNHEKYNGEAIVSNASCTTNCLAPVAK
                     ILNDKWGIEEGLMTTVHATTATQKTVDGPSSKDWRGGRGAAFNIIPSSTGAAKAVGKV
                     IPELNGKLTGMAFRVPTADVSVVDLTCRLAKPATY"
     gene            947..2008
                     /locus_tag="PKK36_09665"
     CDS             947..2008
                     /locus_tag="PKK36_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNR94857.1"
                     /translation="MLTAPAVPEEEISGWPIEESGLPARVVHSTRIAGLSALGDLRAW
                     QDSDLLNLHALGKKSLGQIHEYFGMCDRLAEGRLAFTHFIDLLHAFLDDDEFTVLSFR
                     YGFDLAAPLPSGSSMTLQAIGNRMHRTRERIRQIEETAMRRLESRLCRCSLVPWHDYF
                     TGMLETADGCMACGDLFEHTRRSLFDYSNPCALLLLLCGLSGSRITYRNGFFSLWSAE
                     DLDRVPPEILAWLRQHDGRGRLAEICEHLEKAGLGSRDSIRKHAQLMAEHCADIVVAA
                     DGRIYSADGGISHFLADVLKKFKGPVNYRVIAREANELLRPASRRGTGFWLKALRSHP
                     GIRMRESGRYELSRRASGS"
     gene            complement(1995..2396)
                     /locus_tag="PKK36_09670"
     CDS             complement(1995..2396)
                     /locus_tag="PKK36_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004725227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNR94858.1"
                     /translation="MNTIVKTIVLLVISNTFMTFAWYAHLKNLGHKPWVVAAMVSWSI
                     ALFEYLFQVPANRIGYGALSLPQLKIIQEVITLTVFIPFALIYMRQTWSWNYVWAALC
                     LMGAVFFVFRDPRPAKLPPPAVAVQAPVSSR"
     gene            complement(2414..3469)
                     /locus_tag="PKK36_09675"
     CDS             complement(2414..3469)
                     /locus_tag="PKK36_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR94859.1"
                     /translation="MHFSLEQMAAVALYLLAGELLYIEVVNRWLILIRDGRLKGVIIA
                     GGVLAVAAAAACAGLRLPWWPAAALLAVMLMLEFRRVRMRRAIHGSWPEGPRPHRPSL
                     RRPFTTTDFRIYRYRIELPDWRGRPVRIVHLTDMHVHRRIPDAYFRRVMQAARDEKPD
                     LAFFTGDYLAGQTPPERLRELLGPVGRSGDFAVLGNHDFWDRTEPITRALKDAGIRVL
                     SGESVRVAAGGGELLLTGHDYPWGAQQKTLDPAPPGLPHFVLTHTPDNIYHLAAARPD
                     CVFAGHCHAGQIRLPFWGPVVVPSVYGRRFDHGHFIVDGVHLFIPSGIGVANPLFRIY
                     CEPDFFVIDVRGVGPAV"
     gene            3594..4691
                     /locus_tag="PKK36_09680"
     CDS             3594..4691
                     /locus_tag="PKK36_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNR94860.1"
                     /translation="MKIIDKHLLHTLMVPLAYCLAAFMMIFIVFDLFDNLGDFIDAGT
                     PALDVFKYYVLLMPSLVVYVIPVSLMLAVLYSLSRLTKNNELTAMRASGIGLFRLLAP
                     FVAVGIISSAGVVVIHETIGPWSAYWTNQYVRSQRHKGKLEVYISQNLAFNNARKKRN
                     WLIESFDTRSNLMVNVTIIQQRPDLSDDWKITARRGEWLDGRWWLTDLVKQAYDPNGH
                     PLGPPQFERMMEMTDFNETPQTFINERKDPSVPEQFSSREFRTFLKTHREISGDTVAR
                     IMVDMHHRLALPWMCLIVVLLGIPCGTHTGRKGALLGIAIALSLFFAYYILMNLCLAL
                     GKKQVVEPWLAAWTPNIIFFMVSVIANYRMR"
     gene            4782..5138
                     /locus_tag="PKK36_09685"
     CDS             4782..5138
                     /locus_tag="PKK36_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94861.1"
                     /translation="MIKQNVDPGRNDVSICLDQSGTFRQPVTFRCCPLGIQFYTDDAL
                     QEDLMLEVAVNLPDADGQMQKICCCGVVVQSVLEDTCKGYRNWVYFVDLAPSIKQQLH
                     CLAKESEFLCPYCENF"
     gene            5114..6205
                     /locus_tag="PKK36_09690"
     CDS             5114..6205
                     /locus_tag="PKK36_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNR94862.1"
                     /translation="MSILRELLAKSTADGASDIHLKSGNVPVFRVSGKLIPQGKETVT
                     PEVMDSYLKAILPTRLVRQFEIEHEADFSLAEEGVGRFRVNAFVCQGTANMVLRNIKT
                     TIPTFEQLHLPPQLKQFAAVKRGIILASGTTGSGKSTSLAAIMHEISTTRNCRVITIE
                     DPIEYMFNDDKAIISQREVGLDTMSFHAALKHVLRQDPDVIMVGEMRDAESFMAALSA
                     AETGHIIFSTLHTASAATSIARILDFFPASERDQIRMGLASSVHAVFCQRLIPSIRGG
                     VVPAVEIMINNGTIAKIIRKNKLELLEAAIEVGGPDGMQTFNGSIYDLIRSGQITEEE
                     GMANASNPEALKMRLKGINLDESSRILQI"
     gene            6349..>7055
                     /locus_tag="PKK36_09695"
     CDS             6349..>7055
                     /locus_tag="PKK36_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNR94863.1"
                     /translation="MKIQTRLIAGFGAPLLLLWLLVALALAGGFSPALIIATAALATL
                     AAAAGGVLTARAVLRPVRKLCDEVSRITGNEAEAGITPKGDDEMTDLARVIKVFADRL
                     KQSQTAIEDNLKTQSRMVSEELQELQQKRRELEEGRRKMAQVMDNLRLKQAEMVEEVN
                     QRKLAEARLKNRVAEVEVLNRGITNLLEDLQEANRLQAQTTRRLETANRELESFSYSV
                     SHDLRAPLRAINGFADAL"
BASE COUNT         1499 a         2098 c         2054 g         1404 t
ORIGIN      
        1 ataggtggcg ggctttgcca gacggcaggt cagatccaca accgacacat ccgccgtcgg
       61 aacgcgaaac gccattcccg tcagcttgcc gttcagttcc ggaatcacct tgccgaccgc
      121 cttggccgcg ccggtgctgg aggggatgat gttgaacgcc gcgccgcggc cgccgcgcca
      181 gtctttgctg gaaggaccat ccacggtttt ctgggtggcg gtagtcgcat ggactgtggt
      241 catcagccct tcctcgatgc cccacttgtc attcagaatc ttcgccacgg gcgcgaggca
      301 gttggtggtg caggaggcgt tggaaacaat ggcttcgccg ttgtacttct cgtggttgac
      361 gcccatcacg aacatggggg catccttcga aggggcggaa atcatcacgc gctttgcgcc
      421 ggctttgatg tgggcctcgg ccttttccag cacggtgaac acgccggtgg actccagcac
      481 atattccgcg ccgcattcct tccagggaat catggccgga tctttctggg catagactgc
      541 ggttttcttc ccgttgacca ccagatgccc gtccttgaac tcggcggtgc ccttgaagcg
      601 gccgtgcacg gtgtcatact tcagcatgta caccatgtaa tcgccggaga taaagggatc
      661 attgacgccc accacttcca cgtccggatt cttgactgcc gcgcggaaaa cgaggcggcc
      721 gatgcggcca aaaccgttaa taccaacctt aattgccatt ttctgttcct cctgaattgt
      781 cttctcaatg attaaacgtt ttaccaaagg agcgcataag ttatccgatg agtttttatg
      841 cgtcaatgag aactgaaata ttttttccgc ggaacggaac ggggaaaacg cgcgggaagt
      901 acttttattc ttgacatttt tgccctgttt ttgcactagt gaaagcatgt taactgcacc
      961 tgcggttccg gaagaggaaa tcagcggctg gcccattgag gagtccgggc tgcccgcacg
     1021 agtggtccac agcactcgca tcgcggggtt gtccgccctg ggggatctcc gggcatggca
     1081 ggattccgac ctgctcaatc ttcatgcgct ggggaagaaa tcgctgggcc agattcatga
     1141 gtatttcggg atgtgcgacc ggctggccga aggaagactg gccttcacgc atttcattga
     1201 tcttctgcat gcgttcctcg atgacgatga attcacggtg ctttccttcc gttacggctt
     1261 tgacctcgcg gcccccctgc catcggggag ctccatgacc cttcaggcaa tcggcaaccg
     1321 gatgcaccgc acgcgtgagc gcatccgcca aatcgaggag acggcgatgc ggcggctcga
     1381 gagtcgtctg tgccgctgca gtctggtgcc ctggcacgac tatttcaccg gcatgctgga
     1441 aaccgcggac ggctgcatgg cctgcggcga tctctttgag cacacccgcc ggtccctgtt
     1501 cgattattcc aatccctgtg ccctgctgct gctgctgtgc gggctgagcg ggtcacggat
     1561 cacctatcgc aacggattct tcagtctgtg gtccgcggag gatctggacc gcgttccccc
     1621 ggaaatcctc gcgtggctgc gtcagcatga cggtcgggga cggcttgcgg aaatatgcga
     1681 gcacctggag aaagccggcc tgggcagcag ggattcgatt cggaaacacg cgcagctgat
     1741 ggccgagcat tgcgccgaca tcgtggtggc ggcggacgga cggatataca gtgctgacgg
     1801 gggaatctct catttcctcg cggatgtgct gaaaaagttc aagggccccg tgaactatcg
     1861 cgtcatcgcg cgggaggcca acgagctgct gcgccccgca agcaggcggg gcaccggatt
     1921 ctggctgaag gcgctccgct cgcatccggg aatccgcatg cgggaaagcg gtcgctatga
     1981 actgagccgg cgggctagcg ggagctgacc ggcgcctgaa ctgcgaccgc gggcggcgga
     2041 agctttgcgg ggcgcggatc acgaaagaca aaaaacaccg cgcccatcag gcagagggcg
     2101 gcccagacat agttccatga ccatgtctgg cgcatgtaaa tcagggcaaa tgggatgaac
     2161 acggtcagcg tgatcacctc ctggatgatc ttgagctggg gaagcgaaag tgcgccgtag
     2221 ccgatgcgat tggcgggcac ctggaacagg tattcaaaaa gcgcgatgct ccagctcacc
     2281 atcgcggcga cgacccacgg cttgtgcccc agattcttaa gatgcgcata ccatgcgaac
     2341 gtcatgaatg tatttgagat caccagcagc acaatggttt ttacaatggt gttcataaaa
     2401 tgcttttccg tcgtcaaacg gccgggccga ctcctctgac atcaatcaca aaaaaatcag
     2461 gctcacaata tatccggaac agcggatttg caacaccgat accgctggga atgaagaggt
     2521 gaaccccgtc cactatgaag tgtccgtgat caaaccggcg gccgtagacc gacggcacaa
     2581 cgaccgggcc ccagaaaggc agacggatct gtcccgcatg acaatgtccg gcgaagacac
     2641 aatccggccg cgccgccgcc agatggtaga tgttgtcagg ggtatgggtg agcacaaagt
     2701 gcggcaggcc cggcggcgca ggatcgaggg ttttctgctg cgcgccccat gggtagtcgt
     2761 ggccggtcag caggagttcc ccgcctccgg cggccacccg gacggattcg ccggagagca
     2821 cacggatacc ggcatccttc agggcccggg ttatcggctc tgtgcgatcc cagaagtcat
     2881 gattgcccag aacggcaaaa tcgccggagc ggcccacggg tccgagcagt tcacgcagtc
     2941 tctccggcgg agtctggccg gccagatagt cccccgtgaa aaaggccaga tcgggctttt
     3001 catcccgggc cgcctgcatc acgcgccgga aataagcgtc aggaatccgg cggtgcacgt
     3061 gcatatcagt cagatgcaca atgcggaccg gacggccgcg ccagtccggg agctcaatcc
     3121 ggtagcggta aatccggaaa tcggtcgtcg taaagggccg tcgcagcgac ggccggtgcg
     3181 gacgaggccc ttccggccag cttccgtgaa tggcccgccg catgcgcact ctccggaatt
     3241 ccagcatcag catgacagcc agcagcgccg cggccggcca ccaaggaagc cgcagccccg
     3301 cgcatgcagc ggcggccgca acggcaagca caccgccggc aatgatcact cctttcaggc
     3361 ggccgtcgcg gatcaggatc agccagcggt tcacgacttc aatatagaga agttcgcccg
     3421 ccagcagata aagagcgaca gccgccatct gttccaaaga aaaatgcatt tacagtctca
     3481 tcctatgaca cccgcagggc cgcagccgat caaaaaaacg cgcgcggccc tgcctcctgc
     3541 tgtttttcat ttcacgattg actcggaagc tttgaaggtt aatgttcctg cgcatgaaaa
     3601 tcatagacaa acaccttctg cacacgctga tggtgccgct ggcctactgc cttgcggctt
     3661 tcatgatgat tttcatagtt ttcgacctgt ttgacaacct gggcgacttc attgatgccg
     3721 gaactcccgc gctggatgta ttcaagtatt atgtgctgct catgccctcg ctcgtggttt
     3781 atgtcatacc ggtctcactg atgctggccg tgctctacag cctcagccgg ctgaccaaaa
     3841 acaacgaact cacagccatg cgcgcgagcg ggattggact gtttcgcctg ctggccccct
     3901 ttgtggccgt cgggattata tccagcgcgg gagtggttgt catccacgag acgatcgggc
     3961 cctggtcggc ctactggacc aaccagtatg tgcggtcgca gcgccacaag gggaaactgg
     4021 aggtctacat ttcccagaac ctcgccttca acaatgcccg gaagaagagg aactggctga
     4081 ttgagtcctt cgacacccgc tccaacctga tggtcaatgt cacaatcatc cagcagcggc
     4141 ccgatctttc cgacgactgg aaaatcaccg cacggcgagg cgaatggctg gacggccgct
     4201 ggtggctaac tgatctggtc aagcaggcgt atgatcccaa cggtcatccc ctcggcccgc
     4261 cccagttcga gcgcatgatg gaaatgacgg acttcaatga aaccccgcaa accttcatca
     4321 acgaacgcaa ggatccgtcg gtcccggagc agttttcgtc ccgtgaattc aggacattcc
     4381 tcaaaactca cagggagatt tccggggata ccgtggcgcg catcatggtc gacatgcatc
     4441 accgcctggc cctgccctgg atgtgcctga ttgtggtgct cctgggcatc ccctgcggca
     4501 cccataccgg ccggaaaggc gcgctgctgg gcattgccat cgccctttcg ctgttctttg
     4561 cctattatat actgatgaac ctatgtctcg cgctcggaaa aaaacaggtt gttgaaccgt
     4621 ggctggccgc atggaccccg aatatcatct tcttcatggt ctcggtcatt gccaattacc
     4681 ggatgcgctg atttttccgc gagggtgcat ttgacaaagg ctggagggta ttttacattt
     4741 tcacgttgat ggcatccgca tgtgcggcag gacaagtagt tatgattaaa caaaacgttg
     4801 acccgggcag aaatgatgtt tcgatctgcc ttgaccagtc cggaactttc cgtcagccgg
     4861 tcacattccg ctgctgtccg ctcggaatac agttttacac ggacgacgct ctgcaggagg
     4921 acctgatgct cgaggtggcg gtgaatctgc ctgacgcgga tggacaaatg cagaaaatct
     4981 gctgctgcgg tgtggtggtg cagtccgtgc ttgaagacac atgcaagggc taccggaact
     5041 gggtctattt tgtggatctg gcccccagca tcaaacagca gcttcactgt cttgcaaagg
     5101 aatcggaatt tctatgtcca tactgcgaga acttctagcc aagtcaactg ccgacggcgc
     5161 atcagacatc cacctcaaat ccggcaacgt gccggttttt cgtgtcagcg ggaagctgat
     5221 cccccagggc aaggaaaccg tgacgcccga ggtcatggac agctatctca aggcgattct
     5281 cccgacgcgg ctcgtgcgcc agttcgaaat cgagcatgaa gccgactttt cgctggccga
     5341 ggagggcgtc ggacgtttcc gcgtgaatgc ctttgtatgc cagggcacgg ccaacatggt
     5401 gctccgcaac atcaagacca ccatcccgac gtttgaacag ctccatctcc cgccccagct
     5461 taagcagttt gccgccgtca agcgcggcat cattctggcc agcggcacca ccggctcggg
     5521 aaaatccacc tcgctggccg ccatcatgca cgagatttcc accacccgga actgccgcgt
     5581 gatcacgatt gaagacccca tcgaatacat gttcaacgat gacaaggcca tcatctccca
     5641 gcgcgaagtc gggctcgaca cgatgtcgtt tcatgccgcg ctcaagcatg tgcttcggca
     5701 ggatccggac gtgatcatgg tcggagaaat gcgcgatgcc gaatcgttca tggccgccct
     5761 gtccgcggcg gaaaccggcc acatcatctt cagcacgctt cacacggctt cggccgccac
     5821 atcaatcgcg cgtattctcg acttctttcc cgcatccgag cgcgaccaga tccgcatggg
     5881 actggccagc agcgtgcatg ccgtcttctg ccagcgcctg atcccctcca tccgcggcgg
     5941 ggtggtgccc gcggttgaaa tcatgatcaa caacggaacg atcgcgaaga ttatccgcaa
     6001 gaacaagctg gaactgctcg aggcggccat tgaagtcggc gggccggacg gcatgcagac
     6061 cttcaacgga tccatctacg acctcatccg gtccggtcag atcactgaag aggaaggcat
     6121 ggccaacgcc agcaatcccg aagccctgaa aatgcgcctc aagggcatca acctcgacga
     6181 gtccagccgc atcctgcaga tctgaccgga agttccaggc attggaactt ttcccgggaa
     6241 aacttccagg gattggaact ttttcgggaa aatcttccaa tggttggaac ttttttctac
     6301 cgcaggcacc gggtatagaa tattctgttc gcggatggca cggacgtcat gaaaattcag
     6361 accagactga tcgcaggatt cggggccccc ctgctcctgc tgtggctgct ggtcgcactg
     6421 gcgctggccg gaggcttcag ccccgccctg attatcgcaa cggccgcgct ggcgactctg
     6481 gcggcggcgg ccggcggcgt gctgaccgcc cgtgcggtgc tccggcccgt gcgcaaactg
     6541 tgtgacgagg tcagccgcat aaccggaaat gaagcagagg ccgggattac ccccaaaggc
     6601 gacgatgaaa tgaccgacct cgcccgcgtc atcaaggtct ttgcagaccg gctcaaacag
     6661 agccagacgg ctatcgagga taatctcaaa acgcagagcc ggatggtgtc ggaggagctg
     6721 caggagctgc agcagaagcg ccgggaactg gaagaaggca gacggaaaat ggcgcaggtc
     6781 atggacaatc ttcggctcaa gcaggcggaa atggtggagg aagtgaatca gcggaagctg
     6841 gccgaggcgc gcctgaagaa ccgcgtggca gaggtcgagg tgctgaaccg gggcatcacc
     6901 aatctgctgg aggatctgca ggaggccaac cggcttcaag cccagacgac gcgccggctg
     6961 gagacggcca accgggagct cgagtctttt tcctattccg tatcgcacga cctgcgcgcc
     7021 ccgctgcgcg ccatcaacgg ctttgcagac gcgct
//
LOCUS       DAOQWR010000131         6778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_11612_length_6778_cov_6.135654, whole genome shotgun sequence.
ACCESSION   DAOQWR010000131 DAOQWR010000000
VERSION     DAOQWR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11612_length_6778_cov_6.135654"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKK36_09700"
     CDS             complement(<1..200)
                     /locus_tag="PKK36_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019554684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNR94864.1"
                     /translation="MKQVEFKSLTPVQQTLVNRAVEIRQRAYAPYSNYKVGVALVDEK
                     DNIHAGCNIESADYTLTTHAEM"
     gene            complement(205..975)
                     /gene="udp"
                     /locus_tag="PKK36_09705"
     CDS             complement(205..975)
                     /gene="udp"
                     /locus_tag="PKK36_09705"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HNR94865.1"
                     /translation="MGEISSDVQYHIGLKKGDVGRYVILPGDPGRVPKIAGYFDDAKE
                     MANNREYRTYTGSVDGIKVSCTSTGIGCPSTAIAIEELIKIGADTFIRIGTAGSLQKE
                     VGLGDVCVTTAAVREEGTTQQYVPLSYPAVADLDVTWALREGAKKLGLKVHAGIGQCK
                     DAFYTEGKEGEELPLANYNRDRWTAWYRSNVISTSMESAALFVVSSIRRVHAGEILAI
                     IGLTYDDKPIVAKVGVDDAIKAAIEAIKILEKMRNPKK"
     gene            1286..2632
                     /locus_tag="PKK36_09710"
     CDS             1286..2632
                     /locus_tag="PKK36_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNR94866.1"
                     /translation="MRWTAIICGVLFAVTGFAKVDLVTLPVREGVQLTIYNSADLTLV
                     RENRALTLREGANKLQFSWANTLIDPTSLEMIPLAFAADLDVADLTYPPRTRNLGLWN
                     VKSGRSGKVPVEINYLTSGFSWRAFYMGTLSEDEKTIRLQGYVRVTNNSGEDYENAET
                     RLIVGKVNIIDRIADLARRDYPYGQPWKLPQEDDKDYSRMKKSRREMKNEVMMAMAPA
                     PSSGVTMMRQKEIRKEGLSEYFLYTIEGTETIPHGWSKRLPSLDVADVPVVNLYKFEE
                     DRYGERTVRFLSFKNDKEHKLGDTPIPDGSIRVYRTVDEAAHLSYEGRASFKYIPVDE
                     KVELNIGAVSDVTVEPKLMDYKTDQYQFDRKGNIRGWDEIRTFEVEVKNMRPVPVKIE
                     IIRNFHSPYWDLKKSGDFGEFEKTDLDTVKFTLELPPHSEKKFEYTLILHQGDRRN"
     gene            2646..3770
                     /locus_tag="PKK36_09715"
     CDS             2646..3770
                     /locus_tag="PKK36_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94867.1"
                     /translation="MRKCKWWMLVLLAAAAAQVSEARIKLVALPDRGDTVIRLDNPAA
                     TLIEEERVLTLQKGVNKVDFAWNGVSLDVDSIRLTVLSHPEQVKLLNVSYPPNEQALV
                     WELASPEALEVKARISYLLNGIDRLITYKAVADKEETQVDLKSHLVLRNFSGEDFTSA
                     RVLLNYGEAFEQGIQHEETKQLLFMKQDGIPIEKIWTFDAAKKPWDPEKVSGNVGIPV
                     TYKIKNEKESKLGENSLWDGKVRVYQDDGHEGTIFLGEDNTALVPVGEEMEVYVGDSR
                     DLVITQRKMQEKQINIRRNNSNHIVLYDTDEIITAKIENFKDKPAKLLLIQHIPGQWE
                     MEKCSMEYERKDAGTLEFTVELKPQETRELTMHYHRRNVR"
     gene            3767..5113
                     /locus_tag="PKK36_09720"
     CDS             3767..5113
                     /locus_tag="PKK36_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94868.1"
                     /translation="MKKFLLLIIALAACEARAKVDLVTLPVRDRVQLTIYNSADLTLV
                     RENRALTLVGGLNKLQFSWANTLIDPTSLEMRPLENAGDIDIMALTYPPRTVNLGLWN
                     IKSARGGKVPVEIDYLTSGLSWRAFYMGTLTEDEKTMRLEGYVRATNNSGEDYADAET
                     RLIVGEINLIDQIRMLAQRQYPYDRPSQYPVSPMVMKGLFAGRAGGKELEVLEEAVAY
                     DSFAAEMPETKEIAKEGLSEYFLYTIPGTETIPNGWSKRLPSFDTNGIPVLNLYKYEE
                     ERYGKGVLRFLSFKNDTEHKLGETPIPGGMLKVFRTTDEDGHLAYEGQSDFKYIPVNE
                     DVELNLGYVGDVVVEPKLMDYKTDRFSFDSHGNIDGWDEISTWKVEIKNTRDLPVKVE
                     ITRNTGMPNWDIERRDSVGKYEKVDLDSMKFTVEPEPRSNVEFTYVLTKHFGRRAE"
     gene            5123..5491
                     /locus_tag="PKK36_09725"
     CDS             5123..5491
                     /locus_tag="PKK36_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94869.1"
                     /translation="MKKWIIVFLLLAAASAMADEYLPSAGKYKTCKNEEGRGFYWVDS
                     STGRTWRADPDKKEFVYCGQPPQGTPSEPGTYKPYKNKDGKGLFILNTATGDGWWTDG
                     KEWEKMPKDLKKTERGGKDG"
     gene            5484..5897
                     /locus_tag="PKK36_09730"
     CDS             5484..5897
                     /locus_tag="PKK36_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR94870.1"
                     /translation="MVNCRECQKEVSEQAFSCPHCGAPYPAKPVWDGWGYEYKSKTSI
                     AGLPLIHISFKYRPNRAPVVARGVIAIGQFSVGIINISQFGLGVVCISQFAIAGYALA
                     QLAVAWRLIAQVGLYLSEGRGQAVYSLIELAQKFF"
     gene            6026..6424
                     /locus_tag="PKK36_09735"
     CDS             6026..6424
                     /locus_tag="PKK36_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94871.1"
                     /translation="MSLRHPKVTEWEARLKRVFDRIDDILEDKYHGVYRLSPVRPERG
                     ATSNKEADGLFNVGAAFTAGFGSEKGPGYVVDVKMSTLDRVSPEARREIEDEVASLLD
                     DMLHSEFPKGDLEVSRDGHVWKIHGDLSLD"
     gene            6469..>6778
                     /locus_tag="PKK36_09740"
     CDS             6469..>6778
                     /locus_tag="PKK36_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNR94872.1"
                     /translation="MSFTTDFLQEAVEVLNRLSADEIDAVVSKLAEVRARGGRVFVLG
                     VGGSAAAASHFVNDLRKLAHLEAYAPTDNVSELTARINDEGWESCFAEWLKGSHLKAA
                     D"
BASE COUNT         1568 a         1755 c         2109 g         1346 t
ORIGIN      
        1 tccatttccg cgtgcgtggt cagcgtgtag tccgcgcttt cgatgttgca cccggcatgg
       61 atgttgtcct tctcgtccac cagcgccacg cccaccttat aattggaata gggagcgtag
      121 gcgcgctggc ggatttcgac ggcccggttc accagtgtct gctgaaccgg agtgagcgat
      181 ttgaattcaa cctgcttcat gcgattactt ctttggattg cgcatcttct cgaggatctt
      241 gatggcctcg atggcggcct tgatggcatc gtccacgccc accttagcca cgatcggttt
      301 gtcgtcatag gtcaggccga tgattgccag gatttcgccg gcgtgcacgc ggcggatcga
      361 ggacaccaca aagagcgcgg cggattccat ggaggtggaa atcacgtttg accggtacca
      421 cgcggtccag cggtcacggt tgtaattggc gaggggaagt tcttcgccct ctttgccttc
      481 ggtgtagaag gcgtccttgc actggccaat tccggcgtga accttcagtc ccagcttctt
      541 tgcgccttcg cggagcgccc aggtcacatc cagatccgcc acggcgggat agctcagcgg
      601 aacatactgc tgcgtggtgc cttcctcgcg cacggcggcg gtggtcacac agacatcgcc
      661 aaggccgact tccttctgaa gcgagccggc ggtgccgatg cggataaagg tgtcggcgcc
      721 gatcttgatc agttcctcga tcgcgatggc ggtggagggg cagccgatgc cggtggaggt
      781 gcaggaaacc ttgatgccgt ccacggagcc ggtgtaggtg cggtactcgc ggttgttcgc
      841 catttccttc gcatcgtcaa aatagcctgc gatcttcggc acgcgtccgg gatcgcccgg
      901 cagaatgaca tagcggccca cgtcgccttt tttaagacca atgtgatact gaacatcgct
      961 gctgatttcg cccatcttcg tctcctgttt gatgttttca gacgttaggc ccgcaatatt
     1021 ccagaaatgc atgaggaata aaaggcaatt tttctttcgc cgcctgccgg cttgcccggc
     1081 aggagcgagg ggccagggcg ccagccagtg aacaagcatg accttcttca cccgcgacgc
     1141 aaggtcgcgg ggggggcgaa tggagagaac cgatctgtcg gttccgcgat aaaaagatca
     1201 attatatttt tcaaccatgc catgacttga attgtcaaac cccggcagat gtgtctaata
     1261 tagcggcgtg agggacctga atcacatgag gtggacggca atcatttgcg gtgtgctgtt
     1321 tgcggtcacg ggttttgcaa aagtggacct tgtgacgctt ccggtgcgcg agggtgttca
     1381 actcacaatc tacaactccg ccgatctgac gctcgtccgc gaaaaccgcg cgctgacgct
     1441 gcgcgagggc gcaaacaagc tccagttctc gtgggccaac acgctgattg atccaaccag
     1501 cctggaaatg attccgctgg cgtttgccgc tgatctggat gtcgccgacc tgacctatcc
     1561 gccccgcaca agaaatctgg gcctgtggaa tgtcaaaagc gggcggagcg gcaaagtccc
     1621 ggtcgaaatc aactatctga ccagcggctt ctcgtggcgg gccttttata tgggcacgct
     1681 ctcggaggat gagaagacca tccgcctgca gggctatgtc cgcgtgacca acaacagcgg
     1741 cgaggattat gaaaatgccg aaacgcgtct gattgtcggc aaggtgaaca tcattgaccg
     1801 gattgccgat ctcgcgcggc gggattatcc gtacgggcag ccgtggaagc tgccgcagga
     1861 ggatgacaag gactattccc ggatgaaaaa atcccgccgc gaaatgaaaa acgaagtcat
     1921 gatggcgatg gcgcctgcgc cgtcctcggg tgttacgatg atgagacaga aggaaatcag
     1981 gaaagagggc ctgagcgaat atttccttta taccattgaa ggcactgaaa ccatcccgca
     2041 cggctggtcg aagcggctgc cgagtttaga tgtcgccgat gtgccggtcg tgaacctgta
     2101 taaattcgaa gaagatcgtt acggcgaaag aacggtgcgt ttcctgtcgt tcaagaacga
     2161 caaagagcac aagctgggcg atacgccgat tccggacggg agcatcaggg tttatcgaac
     2221 cgtggatgag gccgcgcatc tgtcgtatga gggccgcgcc agtttcaaat acattccggt
     2281 ggatgaaaag gtcgaactga acatcggcgc ggtttcggat gtgacggtcg agcccaaact
     2341 gatggattac aaaacagatc agtaccagtt tgaccgcaag ggcaacatcc gcggatggga
     2401 tgaaatcagg acgtttgaag tggaagtcaa gaacatgcgg ccggtgccgg tgaagataga
     2461 gatcatccgg aattttcact cgccttactg ggacctgaag aaatcaggcg atttcggcga
     2521 gtttgagaaa accgacttgg atactgtcaa attcacgctg gaactcccgc cgcacagcga
     2581 aaagaaattt gaatacacgc tgatcctcca tcagggggat cgccggaatt gaaacggaga
     2641 tgactatgag aaaatgcaaa tggtggatgc tggtcctgct ggccgccgcg gccgcgcagg
     2701 tttccgaggc gagaatcaaa ctggtggctt tgccggaccg cggggacacg gtaattaggc
     2761 tcgacaatcc tgcggccaca ctgatcgagg aggagcgcgt gctcacgctc caaaaaggcg
     2821 tgaacaaggt ggacttcgca tggaacggcg tgagccttga cgtggattcc atccggctca
     2881 cagtgctgtc gcatccggag caggtgaagc tgctcaacgt cagctatccg ccgaatgagc
     2941 aggcgctggt ttgggagctg gccagtccgg aggcgctgga ggtcaaggcg cggatcagct
     3001 atctgctgaa cggcattgac cggctcatca cctacaaggc cgtggccgac aaggaggaga
     3061 cgcaggtgga tttgaagagc catctggtgc tccgcaattt ttcaggcgag gattttacgt
     3121 ccgcgcgcgt gctgctgaac tacggcgagg cttttgagca gggcatccag cacgaggaaa
     3181 ccaagcagct cctgttcatg aagcaggacg gcattccaat cgagaaaatc tggacctttg
     3241 acgcggccaa aaaaccgtgg gatcccgaaa aagtatccgg caatgtgggc attccggtga
     3301 cctacaagat caagaacgaa aaggaatcaa agctcggtga aaattctctg tgggacggca
     3361 aggtgcgcgt ctatcaggat gacggccatg aaggaacgat attccttggc gaggacaaca
     3421 cggcgctggt gccggttggc gaggaaatgg aggtttatgt cggcgacagc cgcgaccttg
     3481 tcatcacgca gcgcaagatg caggaaaagc agatcaacat ccggcgcaac aacagcaacc
     3541 acattgtgct gtacgacacc gatgaaatca tcacggccaa aatcgagaac ttcaaggaca
     3601 agcccgcgaa gctgctgctg atccagcaca tccccggcca gtgggagatg gaaaagtgca
     3661 gcatggaata tgagcgcaag gatgccggca cgcttgaatt caccgtggag ctgaaaccgc
     3721 aggaaaccag agagcttacg atgcattatc acaggaggaa cgtacggtga aaaagtttct
     3781 tctgctcata atagcattgg ccgcctgcga ggcgcgggcc aaggtggacc tggtgactct
     3841 tcctgtgcgc gaccgggtgc agctcacgat ctacaactcg gccgatctga cgctcgtgcg
     3901 cgagaaccgc gcgctgacgc tggtcggggg gctcaacaaa ctgcagttct cctgggccaa
     3961 cacgctgata gacccgacca gccttgaaat gcggccgctg gaaaatgcgg gcgacataga
     4021 catcatggcg ctgacctatc cgccgcgcac agtcaatctc gggctgtgga acatcaaaag
     4081 cgcgcgcggc gggaaagtgc cggtggagat tgattatctg accagcggct tgtcgtggcg
     4141 cgcgttctac atgggcacgc tgaccgaaga cgagaagacc atgcgtctgg agggctacgt
     4201 tcgcgcgacc aacaacagcg gcgaggatta cgccgatgcc gagacgcgcc tgatcgtggg
     4261 cgagatcaac ctgattgacc agatcaggat gctggctcag cggcagtatc cgtacgaccg
     4321 gccgtcccag tatccggtgt ccccaatggt gatgaaaggt ctgtttgcag gacgagccgg
     4381 ggggaaagaa ttagaggttc tggaagaggc cgtggcgtat gattcttttg cggcagagat
     4441 gccggaaacc aaagagattg cgaaggaggg cctgagcgaa tatttcctct acaccattcc
     4501 cggcacggag acgataccga acggctggtc gaagcggctt ccgagtttcg acaccaacgg
     4561 cattccggtg ctgaaccttt acaaatacga ggaagagcgc tacggcaaag gggttctgcg
     4621 cttcctgagc ttcaagaacg acacggagca caagctgggc gagacgccga ttccgggcgg
     4681 catgctgaag gttttccgga ccacggacga ggacggacat ctggcctatg aggggcagtc
     4741 ggacttcaag tacatcccgg tcaacgagga tgtggagctg aacctcggtt atgtcggcga
     4801 tgtggtggtc gagcctaaac tgatggacta caagaccgac cgcttctcct ttgactcgca
     4861 tggcaacatt gacggctggg atgagatcag cacctggaaa gtggagatca aaaacacgcg
     4921 cgacctgccg gtcaaagtgg agatcacccg caacacgggg atgccgaact gggacatcga
     4981 gcgcagggac agtgtcggga aatacgaaaa ggtggatctc gacagcatga aattcaccgt
     5041 ggaaccggaa ccgaggtcga acgttgaatt cacatacgtc ctgaccaaac acttcgggcg
     5101 cagggctgaa taggagggca tcatgaaaaa gtggatcatt gtgtttctgc tgctggcggc
     5161 agccagtgcg atggcggatg aatatctgcc ttcggcgggc aaatacaaaa cctgcaaaaa
     5221 tgaagagggc aggggtttct actgggtgga cagttccacg ggccggactt ggcgggcgga
     5281 tccggacaag aaggaattcg tctactgcgg ccagccgccg caggggacgc cttcggagcc
     5341 gggcacctac aagccctata aaaacaagga tggcaaagga ctcttcattc tcaacacggc
     5401 caccggcgac ggctggtgga ccgacggcaa agagtgggaa aaaatgccga aggatttgaa
     5461 gaaaacagaa agaggaggga aggatggtta attgtcgcga gtgtcagaag gaagtcagcg
     5521 agcaggcgtt ttcatgtccg cactgcggcg cgccatatcc ggcaaagccg gtttgggacg
     5581 gatggggtta tgaatacaag agcaaaacca gcattgccgg tctgccgctg attcatattt
     5641 ccttcaagta ccgcccgaac cgcgcgccgg tggtggccag gggggtgatt gcgatcggcc
     5701 agttttcggt gggcatcatc aacatttcgc agttcgggct gggggtggtg tgcatcagcc
     5761 agtttgccat agccggctac gcgctggcgc agctcgcagt ggcgtggcgg ctgattgcgc
     5821 aagtcggcct ctatctgtcc gagggccgcg gccaggcggt ctacagcctc attgagcttg
     5881 cacagaagtt cttctgaaaa agtcccaatc cctggaactt ttcgcgaaaa aacttccaat
     5941 ggttggaact ttttgcgtaa aaacttccaa tcattggaac ttttttgaac agaagttcca
     6001 gtcattggaa aaacggagag acctggtgag tttgagacat ccgaaggtga cggaatggga
     6061 ggcccgcctc aagcgggtgt tcgaccgcat tgatgacatc cttgaagaca agtatcacgg
     6121 ggtgtaccgg ctgagcccgg tgcggccgga gcggggggcg acatccaaca aggaggccga
     6181 cgggttgttc aatgtcgggg cggcgtttac ggcgggcttc gggtccgaga aggggccggg
     6241 ttacgtggtg gacgtcaaaa tgtccacgct ggaccgtgtg tccccggagg ctcgccggga
     6301 gattgaagac gaggtggctt cgctgctcga tgatatgctt cactctgaat ttccgaaagg
     6361 cgaccttgag gtgtcccgtg acgggcatgt gtggaagatt cacggcgacc tgagcctgga
     6421 ctgaaacttg gtttgatctc cgtccgcgaa tctggttaat tggtgcgcat gagcttcact
     6481 acagatttcc tgcaggaggc cgtggaagtg ctcaaccggc tctccgcgga cgaaattgac
     6541 gccgtggttt caaaactggc ggaggtgcgc gcgcgcggcg gacgggtgtt tgtgcttgga
     6601 gtgggcggca gtgccgcggc ggcctcgcat tttgtgaacg acctgcgcaa gctggcgcac
     6661 cttgaagcct atgccccgac cgacaacgtt tccgaactca ctgcccgcat taatgacgaa
     6721 ggctgggagt cctgcttcgc ggagtggctc aagggctcgc atctgaaggc ggccgatg
//
LOCUS       DAOQWR010000132         6607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12009_length_6607_cov_5.425519, whole genome shotgun sequence.
ACCESSION   DAOQWR010000132 DAOQWR010000000
VERSION     DAOQWR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_6607_cov_5.425519"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2708
                     /locus_tag="PKK36_09745"
     CDS             <1..2708
                     /locus_tag="PKK36_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94873.1"
                     /translation="NPVIEKLDRFGRPMWSTNGSGIRCNLKGNPVAAIGPNFLECLPD
                     GQGGVVVFWAPVEGAEYRLLSAQRFSAEGERLWGDYGKTNLAYWGGSIPDENVMLLLP
                     SGTNGIVAVWQVSAHVCIPVATLFDWDGNTVWSNLPFGTARGENIMDVCADEIGGTLY
                     VKPLDDGDEGRSCTTMVQRLDEDGTILWGDGIPLAGLYDLYEIQKTWPRIAADGSGGA
                     VVIYNDKDHRGNVHAQRLDAGGNMLWDTNGIIVCSNATSVTVMATCLVRVEPDTWVAV
                     WSDNRTGYYNVYAQKVSGTGVVAWTENGLPVCTVPENHAWQDQSFEWSNKQQLQAIPD
                     GEGGVWIAWLSSYGNQGYYLQHLDENGNTLLPGTARGFADGIPVSYGVSVQDGFSLAA
                     WRPNAVMAAWDTGDPEVRVGTFYSGAPAITSSLTSAATVGTTLTLTNMDGVLFQPGVQ
                     VYLQRPGESIIGAMDVSVDASDNLICTFNFGEDWTARDSSRAWRGIACSDDGANWIAS
                     AGGSDYLHLSTNGGATWAAAASSLDWRGVAASSDGRALAGVAYGGPIYISRDASATWD
                     ARDSSHNWMAIASSADARHLIAAGFNDTLYLSSDYGAAWTPVESNRPWYGVACSDDGS
                     RWIAAAGGPASSNRLVVSGDYGATWTIRDDERQWRAVASSADGSTLAAVVYGGKIYTS
                     DDYGVTWTARDSDRYWTGIACSDDGKRMVACAGGSAYSDQIYVSGDGGVTWTAEGPAE
                     NWVPIACSSDGRRIVAGDYNGQLYTRVLEPETGLWLLTVENSDGQTTSMAFDLRPETY
                     PPDTLGKSIDPGLQLLGCPFGSDIALSEITLTNGTRGATFFEGDNIMIYNSASGTFSN
                     YWLDSSGAWVDNSGPATNVMIDPGGGFWYRSRGGSSFNWNETRPW"
     gene            2720..3610
                     /locus_tag="PKK36_09750"
     CDS             2720..3610
                     /locus_tag="PKK36_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94874.1"
                     /translation="MKLFYIVLLSLVLRTLNSEAALVWGNSGAITNERGVTVASSETD
                     SGIGSFAQLIWAGANETADPFVRSDTGVSGDDAVVATVFSAEDFFSAPMPGIFPMRVA
                     LGAADSNKVYYVRVFNAPNPDYAAGIAASAPLHATYFWQSDTHAYAHNELLDDHWNFA
                     PFGGQTLARGMIASNDVPVWWLVQYDLTNDYDAAAVGNQDIDPLTTDEEWIADTSPID
                     SSSCFRVTSVTYSPSAAVHFNSSTGRLYTMQCVTLLPESNWTNVPGCGPRPGAGGADS
                     MPDTNAAPAGSFYRLSVQLP"
     gene            3833..5497
                     /locus_tag="PKK36_09755"
     CDS             3833..5497
                     /locus_tag="PKK36_09755"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNR94875.1"
                     /translation="MQTRAFLRLFIFLLALASGNPEVHAQVSLEYSSFLGGIGTDDAQ
                     AVALAPDGCIWVCGYTLSTNFPLVNPLDSTLGGSQDGFVSKLSPDGGTLLFSTYLGGS
                     AEDIAAGIAVDANGDVYICGRTDSSDFPTRNAFQPARNGFWTDDFICKLDANGTNLIY
                     SSYLGGSFFDYAYDVAVDTSGCAYVAGMAWSTDFPTVNPFQPTNTLSSGYNFTLTKVA
                     ANGGSLVYSTYLGGSGSGHEYPRVAVTPDGIACFSGTTTSTNFPVVNPYQANHAGPSP
                     SSWDAVISVFSADGSSLTYSTYLGGSEDIDQANDIAIGPDGAITVVGTTQSDNFPVTN
                     AVQDSIRGQPDLFVTQLSPEGDKLQFSTYLGGWDWDYGSAVALGDDGSVIAVGIAVSA
                     NYPVYEACQPANAGGYEAAVTRLSPDGTPFVYSTYLGGSGSDYGRAAVIDSNGAAIVV
                     GRTESANFPAVKPFQGNSGGDADAFICRLNLMPLHISGISRTGGAVRVEWAGEGSVLT
                     QRLMRTGSLASPQQWSCIHTCTPGGARCCETTDPSNALFRVDMQRP"
     gene            complement(5609..6202)
                     /gene="thyX"
                     /locus_tag="PKK36_09760"
     CDS             complement(5609..6202)
                     /gene="thyX"
                     /locus_tag="PKK36_09760"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNR94876.1"
                     /translation="MNVELLSITPNPEELIEQAARTCYQSSDKAVPGNAGKFLSKLLA
                     SGHESPFEHASATFRITGCSRAMTHQLVRHRLMSVSQKSQRYVSEKNFEYVIPPSIPP
                     ELEDDYKADMKIIADMYDKWKKRGIKSEDARFVLPNACATDIMITANFREFRHIFKLR
                     CETHAQWEIQEMALRMLKELHARAPSVFQDLADMYLN"
     gene            complement(6199..>6607)
                     /locus_tag="PKK36_09765"
     CDS             complement(6199..>6607)
                     /locus_tag="PKK36_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNR94877.1"
                     /translation="EPTLQPDLIPFMQKIREHGCRVKLDTNGSLPKVLREIVQAKLAD
                     YIAMDIKAPLESYSRLAGIRVDASVIAESIEIIASCGIPHEFRTTRVNPLLSEADCGA
                     IEGLVPPGSRHKWQTFRPENAMDIDLQKHGVEL"
BASE COUNT         1257 a         2006 c         2056 g         1288 t
ORIGIN      
        1 ggaatcctgt catcgagaaa ctggaccgtt tcgggcggcc catgtggtcc accaacggaa
       61 gcggcattcg ctgcaacctg aaaggcaatc ctgtcgcggc cattggcccg aacttccttg
      121 agtgtcttcc cgacggccag ggcggagtcg tggtcttctg ggcgccggtg gaaggtgccg
      181 agtatcgcct tctcagcgca cagcggttct ccgccgaagg cgaacggctc tggggcgact
      241 acggcaagac gaacctcgcc tattggggcg ggtccatccc cgatgagaat gtgatgttgc
      301 tgcttccgtc cggcacgaac gggatcgtgg ccgtgtggca ggtttcggcg cacgtctgca
      361 tccccgtggc cactctcttt gactgggacg gcaacaccgt ctggagcaat ctgccgttcg
      421 gaacggccag gggggaaaac attatggacg tgtgcgctga cgagatcggc ggaaccctgt
      481 acgtgaagcc cctcgatgac ggcgatgagg gcaggtcctg cacgacgatg gtgcagcgcc
      541 tcgacgagga cggaaccatc ctgtggggcg acgggattcc gctggcaggg ctgtacgacc
      601 tgtacgagat acaaaagaca tggccccgga ttgccgcgga cggttccggc ggcgccgtgg
      661 tgatctacaa cgacaaggac caccggggca atgttcacgc ccagcgcctg gatgccggcg
      721 gcaacatgct gtgggacaca aacggcatca tcgtttgcag caacgccacg agtgtgacag
      781 tcatggcgac gtgcctcgtc cgcgtcgagc cggacacctg ggtggccgtc tggtcggaca
      841 accgcaccgg ctactacaac gtgtacgccc agaaggtgag cggcacgggc gtggtcgcgt
      901 ggacggagaa cggcttgccc gtctgcaccg tgcccgaaaa ccatgcctgg caggatcagt
      961 cgttcgaatg gtccaacaag cagcagctgc aggccatccc cgacggcgag ggcggggtct
     1021 ggatcgcctg gttgagctct tacggcaacc agggctacta cctccagcat ctcgacgaga
     1081 atgggaacac gctgctgccc ggaacggcga gaggatttgc cgacgggatc ccagtgagtt
     1141 atggcgtttc cgtgcaggat ggattcagtc tcgccgcgtg gcgcccaaat gccgttatgg
     1201 cggcgtggga caccggcgac cccgaagtcc gcgtgggcac attctacagc ggcgcgccgg
     1261 cgatcacgtc gagcctcacg tcggccgcga ccgtcggcac aaccctcacg ctcacgaata
     1321 tggacggcgt gctattccag cccggagtac aggtgtacct gcagcgtccc ggcgagagca
     1381 tcattggcgc gatggatgtc tccgtcgacg cctcggacaa cctgatctgc acgttcaact
     1441 tcggcgagga ctggaccgcc cgcgacagca gccgcgcgtg gcgcggcatc gcatgctctg
     1501 acgacggcgc gaactggatc gcgagcgccg ggggcagcga ctacctgcat ctctcaacga
     1561 acggcggcgc gacatgggcc gcggccgcca gctctctgga ctggcgcggc gtggcggcgt
     1621 cttccgacgg ccgggcgctc gccggcgtgg cctacggcgg accgatctac atctcccggg
     1681 atgccagcgc cacatgggat gctcgcgact cgtcccacaa ctggatggcg atcgcctcgt
     1741 cggctgacgc acgacacctc attgccgcgg gcttcaacga cacgctctac ctctcctcgg
     1801 attacggcgc agcctggacc ccggtcgaga gcaaccgccc ctggtacggg gtggcgtgtt
     1861 cggacgacgg atcgcgatgg atcgcggccg ccgggggacc cgcgtcatcg aaccgccttg
     1921 ttgtttccgg cgactacggc gctacgtgga cgatccgcga cgacgagcgc cagtggcgtg
     1981 ccgtggcctc ttccgccgac ggcagcacgc tcgccgccgt cgtgtacggc ggaaaaattt
     2041 acacctccga tgactacggc gtcacttgga cggcccgcga ttcggacagg tactggaccg
     2101 ggatcgcgtg ctcggatgac ggcaaacgca tggttgcctg cgcgggcggt tcggcctact
     2161 ccgatcagat ttacgtctcg ggtgacggcg gtgtcacgtg gacagccgag ggtcccgctg
     2221 aaaactgggt gcccatcgca tgctcgtctg acgggcggcg cattgtggct ggcgactaca
     2281 atggccagct ttacacgcgc gtgctcgaac cggaaaccgg cttgtggctg ctgaccgtgg
     2341 agaattcgga cgggcagacc accagcatgg ccttcgatct ccgaccggag acctatccgc
     2401 cggacacact cgggaagtca atcgatccgg gcctgcagct tcttggctgc cctttcggca
     2461 gcgatatcgc gctgtcggag atcacgctca ccaacggcac acgcggggca acattctttg
     2521 aaggtgacaa catcatgatc tacaacagcg ccagcggaac gttcagcaac tactggctcg
     2581 actcctccgg cgcatgggtg gacaacagcg ggcccgcgac aaacgtgatg attgatcccg
     2641 gcggcggctt ctggtatcgc agccggggcg gcagttcatt taactggaac gagacgaggc
     2701 catggtgagg aggcggacaa tgaagctttt ttacattgtt ctcttgagcc tggtcctccg
     2761 cactcttaat tcagaagccg cgctggtctg gggcaacagc ggcgcgatca ccaacgagcg
     2821 aggcgtgacg gttgcctcgt ccgagaccga cagcggaatc ggctcgttcg cgcaactgat
     2881 ctgggcagga gccaacgaaa ccgcggatcc gtttgtccgc tccgacaccg gcgtcagcgg
     2941 cgacgacgct gtggtcgcaa cagtcttcag cgcggaggat tttttctccg cgccgatgcc
     3001 gggcatcttc cccatgcgcg tcgcgctggg cgcggcggac agcaacaagg tgtactatgt
     3061 gcgtgtgttc aatgcgccga acccggatta cgcggcaggc atcgcggcct ctgccccgct
     3121 ccacgcgaca tacttctggc agtcggatac tcatgcctac gcgcataatg aactgctcga
     3181 cgaccactgg aacttcgcgc cattcggcgg gcagaccctc gcccgcggaa tgatcgcgtc
     3241 aaacgacgtg ccggtctggt ggctggtgca gtatgatttg accaacgact acgatgccgc
     3301 cgccgtcgga aatcaggata tcgatcccct cacaactgac gaagaatgga tcgcggacac
     3361 cagtccgatc gacagcagca gctgcttccg cgtgacaagt gtcacataca gcccatcggc
     3421 agcagttcac ttcaactcga gtaccggccg cctgtacacg atgcagtgcg tcaccctcct
     3481 gcccgaaagc aactggacaa acgtgccggg atgcgggccg agaccgggcg cgggcggagc
     3541 ggattcaatg ccggacacga acgcagctcc ggccggttca ttctaccgcc tgagcgtgca
     3601 gctcccataa agaccatgcc gggcgcagca ggggtctcaa gcggcttatg ctgcggactg
     3661 aagacgcaga aggcgggcca catggcactc gcggacataa tccattgcac acaaaagggt
     3721 tattatcatt ctcttcacca ggaaaacggc gttttttgct tgattttata ttgatactat
     3781 gcgtttatat atatgtccgc taaaggagat ctgacgacac aaggaggtcg tgatgcaaac
     3841 aagagccttt cttcgactgt tcatctttct tctcgcgctt gccagcggga accccgaagt
     3901 ccacgcacag gtctcgcttg aatattcctc gttcctggga ggaatcggga ccgatgacgc
     3961 gcaggcggtc gcgctggccc cggacggatg catatgggtc tgcggataca cgctgtcaac
     4021 caacttcccg ctggtcaatc cgctggattc aaccctgggg ggcagtcagg acgggtttgt
     4081 cagcaagctc agtcccgatg ggggcactct tctcttctcg acttatctcg gcggcagtgc
     4141 cgaagatatt gcggcaggca tcgccgtgga cgccaacgga gacgtgtata tctgcggcag
     4201 aacggactcc tcggacttcc cgacccgcaa tgccttccag ccggcgcgga acggtttctg
     4261 gacagatgac ttcatctgca agctggatgc aaacggcacc aacctcatct attcaagcta
     4321 tctgggaggt tcgtttttcg attatgctta tgatgtcgcc gtggacactt ccggctgcgc
     4381 ctatgtggcc ggcatggcct ggtcaacaga tttcccgacg gtcaatccct tccagccgac
     4441 gaatacgctt tcctccggct acaatttcac cctgaccaag gtggcggcca atggcggttc
     4501 ccttgtctac tccacctatc tgggcggctc gggatcgggc cacgaatatc cgcgtgttgc
     4561 cgtcacaccg gacggtatcg cgtgcttcag cggaaccaca acctcaacca acttccccgt
     4621 ggtgaatccc tatcaggcaa accatgccgg tccctccccc agcagctggg atgcggtcat
     4681 cagtgtcttc tcggccgatg gatcatcgct gacctactcc acctatctgg ggggatcgga
     4741 ggatatcgac caggccaatg atatcgcaat cggcccggat ggggccatca cggtggtcgg
     4801 aacaacccag tcggacaatt ttcccgtgac gaacgcggtt caggacagca tcaggggcca
     4861 gccagacctg ttcgtaactc agctcagtcc ggaaggcgac aagctgcagt tttcgaccta
     4921 cctcggcgga tgggactggg actatggatc cgccgtcgcc cttggcgacg acggatccgt
     4981 catcgcggtg ggaatcgcgg tctcggccaa ctatccggtc tacgaggcat gccagcctgc
     5041 gaatgccggg ggatatgaag ccgcggtgac caggctgtcg cccgacggaa ccccgttcgt
     5101 gtattccacc tacctcggcg ggagcgggtc tgactatggt cgcgccgctg tcattgacag
     5161 caacggggcc gcgatcgtcg tgggccgaac ggaatccgcc aacttcccgg cggtaaagcc
     5221 atttcaggga aacagcggcg gagacgcgga tgcctttatc tgccgtctga atctgatgcc
     5281 gctgcatata agcggaatat cgcgaacggg cggcgcggtc cgagtcgaat gggccggcga
     5341 gggcagcgtg ctgacccagc gcctgatgcg aaccggttcg ctggcttctc cgcagcagtg
     5401 gtcctgcatt cacacatgca ctccgggcgg cgcgcgctgc tgcgaaacaa cagatccgtc
     5461 caacgctctc ttccgtgtcg atatgcagag accgtaggac actttgcgca gcgaagctgt
     5521 gcgccgcggc atgcagcggc ctgttataca aaaacctgtg tcgagggatt catcgcctga
     5581 ttgaacctga ttatgccgcg gccgcgattc aattcagata catatccgcc aggtcctgga
     5641 aaacggacgg ggcgcgggcg tggagttcct tgagcattcg cagggccatt tcctggattt
     5701 cccactgggc gtgggtttca cagcgaagct tgaagatgtg gcggaattcg cggaagttgg
     5761 cggtgatcat gatgtcggtt gcgcaggcgt tgggaagcac gaaccttgca tcctcgctct
     5821 tgatcccgcg ctttttccat ttgtcgtaca tgtccgcgat gattttcatg tcggctttgt
     5881 aatcatcctc cagctccggc gggatgctgg gcggaatgac gtattcaaag tttttttcag
     5941 aaacgtagcg ctggctcttc tgcgagacgg acatcaggcg gtggcgcacg agctggtgcg
     6001 tcatggcgcg cgagcagccg gtgatgcgga acgtggcgct tgcatgctca aacggcgact
     6061 catgtccgct ggccaggagt ttcgacagga acttcccggc atttccgggc acggccttgt
     6121 cgctgctctg atagcaggtg cgggcggcct gttcgatcag ttcctccgga ttcggggtga
     6181 tgctcagcag ttcaacattc acaattcaac tccatgcttc tgcaggtcaa tgtccattgc
     6241 gttttccgga cggaaggtct gccatttgtg gcgggagccg ggaggcacca ggccttcgat
     6301 ggcgccgcag tcggcttctg aaagaagcgg attcacgcgg gtggtccgga attcgtgcgg
     6361 gatgccgcag gaggcgatga tctcaatgct ttcggcaatg accgacgcgt ccacgcggat
     6421 tcccgccagc cggctgtagc tttccagcgg ggccttgatg tccatggcga tatagtccgc
     6481 cagtttcgcc tgaacaattt cacggagaac cttcggcagg cttccgttgg tatcgagctt
     6541 gaccctgcac ccgtgctcac ggattttttg catgaaggga atcaggtcgg gctgaagcgt
     6601 gggctcg
//
LOCUS       DAOQWR010000133         6588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12048_length_6588_cov_5.437624, whole genome shotgun sequence.
ACCESSION   DAOQWR010000133 DAOQWR010000000
VERSION     DAOQWR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12048_length_6588_cov_5.437624"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="PKK36_09770"
     CDS             <1..1929
                     /locus_tag="PKK36_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR94878.1"
                     /translation="AAPSVQNPLPEPRIHADARPPSGALSGILVFCSAGHGWTSKDGA
                     WISQRPLLYGVNEDAGNIDQLNLFAQYCFNAGATVVPFRPLGYQTNEVILDNADSGAE
                     YAGTWYDSASSIYFGRSGDVPYRYAFASTNGQTATARFTPRLPEAGFYPVYCWARSGA
                     DRIEQLYRIAHSGGLTEVRVNHERVGLGWVWLGNYHFGAGTNGYVEVSNDALPGRMPE
                     DVVIADAIRFGNGMGDIDRGHGVSGFERELEASRYWIEKSVGQGMSSGIYDLGGYDDQ
                     DDNVGAPRRMTEEMNREQAGGFWDRIFLGFHSNAGSGAARGALGLYDTRYPEDIQKRQ
                     MDFGRALADELNACMEAGDDGVKFSGDWSDASGEVLGGTFGELYGAIGREMNNTIIEV
                     AFHDNEEDCLLMKNPDARGEIARACYQGTVQHLHANNPSNVPLAILPEPPENLRVYGT
                     RQGVVVEWDPPPGEPAAGYTVYSSRNGYGFGSGIVVTGETSTSVMFKSLKPDEVRWFR
                     VAAWNRGGESLPSAMARVRALAAGPGSLEVIYMPARERLQAVRRWFGGGLSGQVTLLR
                     PVDAEISPADIPAVVADAAPIEADLSLAGGADAAPEALPVPRKCSRDNPECIFLPGQD
                     RCLNYFHARECKNPAGVL"
     gene            1971..>6588
                     /locus_tag="PKK36_09775"
     CDS             1971..>6588
                     /locus_tag="PKK36_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNR94879.1"
                     /translation="MNGLNSLFHKVCILGAGAVLFSSSLLSAAVVQLESGAIDYYEPP
                     VMPECPDGREAEVLRRAETSEQEIPPVVPPVSMHQAELEYHLQFDAETESEWDAVQAV
                     QRAELPVRKTAPLKGGASSPGPFTLQKEVFGWHPYWEGTSYTNFTFDLLSTVAYFSYV
                     VDPNTGYATTMNSWSNTPLVEWAHSNNTEAVLCATLFSGHSTFFGSAAAQSNLINELI
                     RVVNLRNADGVNIDFEGVPSGSRSQLTAFMSNLTVKMHAQVPGSQVSICLPAVDWSSN
                     FDVAAFDGFLDKCIMMGYDYSWSKDTQAGPVAPLQTSAVFGSLSVGKSVSNYLAAGLS
                     PGKLLLGVPYYGYDWPTVSYALHANTITSGVSRSYPAAKSFAETYGYNWDTNSLTPYV
                     LYGSYRQLWYDDTNSLARKYDFANSKNLAGIGIWALNKDAGVSDLWDLLAEKFSSSDA
                     QWVTQNSGTSTNFYGVGAKASSFAAVGAAGAIYTSPDSGETWVSRVSGTTSMLLNFIS
                     GGSYWVAVGDAGTIVTSVGGATWTVRTTPTNAMLRGAAYGNGVFVAAGANGTILRSTN
                     ASNWTQQVSGTSENIQGVNYAGGLFIATGENSLLLTSSDGISWTSRTSNAAGWLLDAS
                     YGNGTYVAVGLGSRIVTSPDGTMWTRQTNNLPSGSVNLYRVAFGNGQFVAVGQNGVIW
                     GSTNGINWFEENSGTTNFLRGVVYSNDLFVVSGYNGTILTKGAGAPSVDIGTTGTTVP
                     NSTSSQTITGTANAYVVGTMRWTNSLGGGGTFAAGANWSFSASLAVGTNLITVAGTNS
                     AGSSASDSVTFVRETAGGGEEPAGDELVVTPAPSGALTNIVVFTSAGHGYYANTTSGG
                     WYTGRGLINGVVEDMGNIDQLNTFVQYCLNAGAAVIPCRPVGYQTNEVVLDNDDAGVT
                     YSGSWSDSSSTIYYGSPGDTPYRFSYITTTGQSAVARYTPTILVSGEYPVYTWALYSG
                     NRVRQLYRIRHSGGINEIKVNHRRVGAGWVWLGNYYFEAGTNGYVEISNYAPGYLPGT
                     DVVIADAIRFGNGMGNINRGWGVSGYEKELEASRYWVQNMTGQGMSSDLYDRPTLDDN
                     DDNVGAPPRIANYMDNEADGDFWDRIYLGFHSNADTNVVGIRRGAMGLYSTANSSACQ
                     LRQQAFASALNSEIETDFEFLDNGVGFIDDWTDNSSDVYGASYGEISETYNPNMNHTI
                     IEVAFHNNPEDAKLLKDPSARRYFSRSCYKGLVKHLNANNATVPLNFLPDPPVAPAAV
                     NVSGGVRISWSAPATNIASGQAATGYRVYRSENGYGFGSPVATTNLNVTLTNLNSGAV
                     YFFRVTALNAGGESLPSETIGSRASPAGRAQHLIVNGFDRFDRALSPTQYFGGGISGY
                     VTMVRPRQINSFDYVVQHGQAIAAAGQYFDSASHQAIISSAVRMTNYHAAYWILGEES
                     TADETFSSTEQSLVQSFLTDGGNLFVSGAELGWDLGRTNGVDMTFFTNVLKTAYVSDD
                     AATDKATGRTGSIFSGISALTFSETAGPGDTIYGAEYPDVFQGRG"
BASE COUNT         1333 a         1884 c         1978 g         1393 t
ORIGIN      
        1 gcggctccct cggttcagaa tcccctgccg gaacctcgga ttcatgcaga tgcccgcccg
       61 ccttcgggcg cgctgtccgg cattctggtt ttctgcagcg cggggcacgg ctggacatcg
      121 aaagacggcg catggatatc gcagaggcct ctcctgtacg gggtgaatga ggatgccgga
      181 aatatcgacc agcttaatct ttttgcacag tactgtttca atgccggagc gacagtcgtg
      241 cctttccgtc cgctgggata ccagaccaac gaggtcattc tggacaatgc ggattccggc
      301 gcagaatatg ccggaacgtg gtatgacagt gcatcctcaa tttactttgg acgctccggc
      361 gacgttccat atcgctatgc ctttgcctct acaaacggcc agaccgccac ggcccgattc
      421 acgccgcgcc tgcccgaagc cggattttac cccgtttact gctgggcccg gagcggtgcc
      481 gaccgcattg aacagctcta ccggatcgca cacagcgggg gactgaccga agtccgcgtg
      541 aatcacgagc gcgtcggcct cggctgggtc tggctgggga attatcattt cggggccggc
      601 acgaacggat atgtggaggt ctctaatgat gcgctccccg gaagaatgcc ggaagacgtg
      661 gtgattgccg acgcgattcg tttcggaaac ggcatgggcg acattgaccg gggccacgga
      721 gtctccggct ttgaacgcga gctggaagcc tcccgctact ggatcgagaa atcggtcgga
      781 caggggatga gttcgggcat ttatgacctc ggcggctacg acgaccagga tgacaatgtc
      841 ggcgctccgc gccgcatgac cgaagagatg aaccgcgaac aggccggagg cttctgggac
      901 cggattttcc tcggttttca ttccaacgcc ggaagcgggg ccgcccgcgg cgctctcggc
      961 ctgtacgaca cgcgctatcc tgaagacatt cagaagcgcc agatggattt cggccgggcg
     1021 ctggcggatg agttgaacgc ctgcatggag gccggcgatg acggagtcaa attctccggc
     1081 gactggtcgg atgcttccgg cgaagtgctg ggcggtacat ttggcgaatt gtacggcgca
     1141 atcggccgtg aaatgaacaa cacgatcatt gaggttgcct ttcacgacaa cgaggaagac
     1201 tgcctgctga tgaagaatcc ggatgcgcgc ggggaaatcg cccgcgcctg ctatcaggga
     1261 accgttcagc acttgcatgc aaacaatccg tcaaacgttc cgctggcgat acttccggag
     1321 cccccggaga atctgcgcgt ctatggcacc cgccagggcg tggtggtgga atgggatccg
     1381 ccgccgggcg aaccggccgc gggatatacg gtctattcct cgcggaacgg gtacggtttc
     1441 ggcagcggaa ttgttgtgac gggagaaacc tcgacatcgg tcatgttcaa gagcctgaag
     1501 ccggacgaag tccggtggtt ccgcgtggcc gcgtggaacc gcggcggcga atcgcttccg
     1561 tccgcgatgg cccgcgtgcg cgcgctggcc gccggacccg gcagcctgga ggtgatatac
     1621 atgcctgccc gcgaaaggct tcaggcggtg cgccgctggt ttggcggcgg gctttccggc
     1681 caggtcaccc tgctccgccc tgtggatgca gagatctccc cggcggacat cccggctgtt
     1741 gtggccgatg cggccccaat cgaggccgat ctttcgcttg cgggcggcgc ggatgctgca
     1801 ccggaggcgc ttcccgttcc ccgcaaatgc tcgcgcgaca accccgaatg catcttcctc
     1861 cccggacagg accgctgtct gaattatttt catgccaggg agtgcaaaaa ccccgccggc
     1921 gtgctataaa cagttttggg cccgatttta attttttgca gaggggcgtt atgaacggac
     1981 tcaacagctt atttcacaag gtgtgcattt tgggtgccgg ggcggtgctt ttttcatcat
     2041 cgcttttatc cgcagccgtt gttcagcttg aatcgggagc cattgactac tacgagccgc
     2101 cggttatgcc ggaatgcccc gacggtcggg aggcggaagt ccttcgcaga gcggaaacct
     2161 ccgaacagga aattcctccg gtcgttccgc cagtttctat gcatcaggca gaactggaat
     2221 atcacctgca gttcgatgct gaaacagaga gtgaatggga tgccgtgcag gctgttcagc
     2281 gcgcggaact gccggtgcgc aaaacggctc ctcttaaagg cggggcctca tcgccgggtc
     2341 cattcacgct tcaaaaagaa gtgttcggct ggcacccata ctgggaggga accagctata
     2401 ccaacttcac cttcgatttg ctttcaacgg tggcctattt ttcttatgtg gtggatccca
     2461 acaccggcta cgcgacgacc atgaacagct ggtcgaatac cccgctggtg gaatgggcgc
     2521 attcgaacaa caccgaagct gttctttgcg caactctttt ttccggccac agcaccttct
     2581 tcgggagcgc tgcggcccag agcaacctga tcaatgagct gattcgcgta gtgaacctgc
     2641 gcaatgccga tggcgtgaac attgattttg agggggtccc cagcggttct cgcagtcagc
     2701 tcacggcgtt tatgagcaat ctcaccgtga agatgcatgc acaggtgccg ggatcgcagg
     2761 tttcgatctg cctgccggcg gtggactgga gcagcaactt cgacgtggcg gcctttgacg
     2821 gctttttgga caagtgcatc atgatgggct acgactattc atggagcaaa gacactcagg
     2881 cgggcccggt cgcgccgctg cagaccagtg ctgtgttcgg ctcgttgagc gtgggcaagt
     2941 ccgtcagcaa ttatctggcc gccggtctgt cgccgggcaa gcttctgctg ggcgtcccgt
     3001 actacggcta tgactggcca acggtgtcat atgccctgca cgcgaacaca attacatctg
     3061 gagtttcaag aagttatcct gcagccaaat cttttgccga aacgtatggc tacaactggg
     3121 ataccaactc gctgacgccg tatgtgctgt acggatcata ccgacaactc tggtatgacg
     3181 acacaaacag tctcgcgcgc aagtacgact ttgccaattc caagaatctg gccggcatcg
     3241 gcatctgggc gctcaataaa gatgcgggtg tttccgatct ctgggatctg ctggccgaga
     3301 agttctcttc ttcggatgcg cagtgggtca cacagaactc gggcacttca actaatttct
     3361 acggcgtggg cgcaaaggca agcagctttg ccgccgtggg cgccgccgga gcgatttata
     3421 catcacctga cagcggagaa acctgggtgt cgcgcgtttc gggaaccacc agcatgctgc
     3481 tcaacttcat cagcggagga agctactggg tggcggtcgg cgatgccgga acgattgtca
     3541 cctcggtcgg cggcgcgaca tggacggtgc gaacaacgcc caccaacgca atgctgcgcg
     3601 gcgcggccta cggcaacggc gtgttcgtgg ccgccggcgc caacggcacg attctgaggt
     3661 caaccaatgc gtccaactgg acgcagcagg tgtcgggcac gtcggaaaac atccagggtg
     3721 tgaactatgc gggcggtttg ttcattgcga ccggtgaaaa cagtcttctg ctgacttcgt
     3781 cggacggcat ctcatggacg tcgcggacgt caaatgccgc cggctggctt ctggacgcct
     3841 cctatggaaa cggcacctat gttgccgtgg ggcttggatc ccgcattgtg acctcgccgg
     3901 acggcaccat gtggacacgc cagaccaata acctgccctc ggggagtgtt aatctttatc
     3961 gcgtcgcctt cggaaacggg cagttcgtgg ctgttggtca gaacggtgtg atctggggct
     4021 caaccaacgg catcaactgg ttcgaagaaa attccggcac caccaatttc ctccgcggcg
     4081 tggtctacag caacgatttg tttgtggtgt cgggatataa cgggaccatc ctgacaaagg
     4141 gtgccggcgc gccgtctgtt gacattggaa caacgggcac aactgttccg aacagcacat
     4201 cgagccagac catcacgggc acggcaaatg cctatgtggt gggcacgatg cgctggacga
     4261 atagtctcgg agggggcggc accttcgccg cgggggccaa ctggtcgttt tccgcttcgc
     4321 ttgctgttgg aaccaacctc atcactgtcg ctggcaccaa ttcagccggt tcatccgcga
     4381 gcgacagtgt aacatttgtc cgggagacgg ccggcggcgg ggaagagccg gcgggagacg
     4441 agctggtggt gacccctgcg ccttctggtg ccctgacgaa catagtggtt ttcaccagcg
     4501 cggggcatgg ctattacgcc aacaccactt ccggcggctg gtacacgggc cgcgggctga
     4561 tcaacggggt ggtggaggac atgggaaaca tcgatcagct caacaccttt gtgcagtatt
     4621 gcctgaatgc cggggccgcg gtaattccgt gcaggccggt gggataccag accaatgagg
     4681 tggtccttga caatgacgat gccggcgtga cctacagcgg gagctggtct gacagctcat
     4741 ccaccattta ttacggttcc cccggagata cgccctaccg gttttcctac atcaccacca
     4801 caggacaaag cgcggttgcg cgatacactc cgaccattct tgtcagcggc gagtatccgg
     4861 tttacacttg ggcgttgtat tccgggaacc gggtgcgtca gctctatcgc attcgccaca
     4921 gtggcggcat aaatgaaatc aaagtgaacc atcggcgcgt gggcgccggc tgggtgtggc
     4981 ttggcaacta ttactttgaa gccggcacca acggatatgt tgaaatttca aattacgctc
     5041 ccggatatct gcccggcacg gatgtggtga ttgcggacgc cattcgcttt ggcaatggaa
     5101 tgggcaacat taaccgcggc tggggtgtgt ccggctatga aaaggaactc gaagcctcgc
     5161 gctactgggt tcagaacatg accggtcagg gcatgtcctc tgacctttat gaccggccaa
     5221 cgctggacga caacgacgac aacgtgggcg cgcctccgcg aattgcaaac tacatggaca
     5281 acgaggcaga tggtgatttc tgggaccgca tatatctggg atttcattcc aacgctgata
     5341 ccaacgtcgt cggtatccgt cgcggcgcga tggggcttta cagcacggcc aactcatccg
     5401 catgtcagtt gcgtcagcag gctttcgcgt ccgcgctgaa ttcggaaatc gaaacagact
     5461 ttgaattcct tgataacgga gtcggtttca tagacgactg gacggacaat tcttcggatg
     5521 tttacggggc ctcgtacggt gaaatatctg aaacatacaa ccccaacatg aaccacacca
     5581 tcattgaggt ggcctttcac aacaaccccg aagacgcaaa gctcctgaag gacccctccg
     5641 cgcgcaggta tttctcccgg tcatgctaca aggggctggt gaaacacctc aatgccaaca
     5701 acgcaaccgt gccgctgaac ttcctgcctg atcctcctgt cgcgccggcc gcggtgaatg
     5761 tctccggagg tgtgaggatt tcctggtcgg ctccggcaac caatatcgcc agcgggcagg
     5821 ccgcgaccgg gtaccgcgtg tatcgttctg aaaacggtta tggttttggt tccccggttg
     5881 ctacaacgaa tttgaatgtg acactgacca atctgaattc gggagccgtg tatttcttcc
     5941 gcgtgacggc gcttaacgcg ggtggcgaat cgctcccgtc tgagaccatt ggaagccgtg
     6001 cttctccagc cggccgcgcg cagcatctga ttgtaaacgg gttcgatcgt ttcgaccgcg
     6061 cgctatctcc cacacaatat ttcggaggcg gaatcagcgg ttatgtgact atggttcggc
     6121 ctcgtcagat caacagtttt gattatgtgg tccagcatgg acaggccatc gctgcggccg
     6181 gacagtattt tgattccgca tcgcatcagg caatcatatc gtctgcggtg cgcatgacca
     6241 attatcacgc ggcttactgg attctcggcg aagagtccac ggcagatgaa accttcagca
     6301 gcacggagca gtctctggtg cagtccttcc tgaccgatgg cggaaatctc tttgtctccg
     6361 gcgcggagct tgggtgggac ttgggccgca ccaatggtgt ggacatgact ttcttcacca
     6421 atgtgctcaa gaccgcctat gtcagcgacg atgcggccac cgataaggcc accggccgca
     6481 ccggctccat cttcagcggg atttccgcac tgacgttcag tgagaccgcc ggccccggtg
     6541 ataccattta cggcgcggaa tatcccgatg tctttcaggg tcgcggcg
//
LOCUS       DAOQWR010000134         6541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12153_length_6541_cov_6.053191, whole genome shotgun sequence.
ACCESSION   DAOQWR010000134 DAOQWR010000000
VERSION     DAOQWR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12153_length_6541_cov_6.053191"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PKK36_09780"
     CDS             <1..1337
                     /locus_tag="PKK36_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94880.1"
                     /translation="AAEKAGYATGTPLYVYSETDPVYASEKSGLATGTPVYAETDPVW
                     IAEKGAYATGTPVYTETDPVWAAEKSSYATGTPLYVYTESDPNAVLADGSRAMDGDLD
                     MGGNSITNFSTNSIFFADGTAMGVTDVQNWNSAYGWGDHSAAGYATGTPLYAQSETDP
                     LAVLADGSRPMSGRLDMGGNGITNVNFIAFDTNVQVGDGAQSFSSLGGSVAIGKDATA
                     RYRTSGLAIGTEADGDYNGIAIGYQADGQYSNIAVGFQSSAYSGYDRIAIGCKITNRV
                     NNSCAIRGTLYLDGGTGVMVRSSVGTGAWSAKAFTIDHPLDPENMVLRHFCLEGPEVL
                     NVYNGNAVLDDAGETVITLPDYFEAINRDPRYALTPIGAKMDLYIKQEISDNRFIVAG
                     GAPGGKVSWEVKALRSDPALLEDIRRRPVEQPKSELTPDQMVQENETVNTFP"
     gene            1468..2391
                     /locus_tag="PKK36_09785"
     CDS             1468..2391
                     /locus_tag="PKK36_09785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR94881.1"
                     /translation="MDNLVLVTVNNESLTREEARRIGRDMAARQGIPPQMIDKYLEQA
                     GEELEKHTVEQFINQALIKEEVDRRAVPVSEEEIGAAVAELERSFPEGMTLDKVLVAQ
                     GVSRKAVEEEIIRNERVRKLYETETAGTPSASDEQVSAFYQANRERFRTEESAEVRHI
                     LIGCKAGDDPAVFRTAKEEAAAIREQLLNGADFGKLAAEKSACPSGKQGGSLGDVKRG
                     QMVPAFEEAVFSQTVGEIGPVVTTEFGCHVVQVTARNEAAVIPEEDAKESIREHLSAQ
                     AKGLAFEKFVAGLRGKARITYGGRGGGIIIP"
     gene            2438..2980
                     /locus_tag="PKK36_09790"
     CDS             2438..2980
                     /locus_tag="PKK36_09790"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNR94882.1"
                     /translation="MKTIPIGKAFTLMEPGPVVLVVTRDGRKNNIMTISWTMVMDFTP
                     ILAITTGPWNYSFAALQKTKECVISIPAVDLLDKVVGAGTCSGADTDKFEKFGLTPVK
                     GKHVRPPLIRECVANIECRVIDIVKKHNIVVLEGVAAHLNGSRKEKRTIHAIGDGTFV
                     VDGRKLDRRKMMASRLPYGI"
     gene            complement(3015..3091)
                     /locus_tag="PKK36_09795"
     tRNA            complement(3015..3091)
                     /locus_tag="PKK36_09795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3055..3057),aa:Arg,seq:cct)
     gene            3158..4102
                     /locus_tag="PKK36_09800"
     CDS             3158..4102
                     /locus_tag="PKK36_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR94883.1"
                     /translation="MNCATITNFEAYENCRLCPRECGVDRAAGRTGVCEETAECRIAS
                     AGPHFGEEPCFSGTRGSGTVFFTGCSTGCFFCQNWQISHEGAGHVYDRESFYALLKAL
                     AAKDVHNINFVTPDHFWPHIAWACSRLRTEGVSIPFLFNSSGYHREDMIEAYAGHMNI
                     FMPDFKFADPELAQQCMQDRRYPDIALKAIRKMVELKGFLTPWDPSGETTAVSGVLVR
                     HLVLPGHIQNSIDALHLLKNEFGPLLPISVMSQFRPTRLCTARDCLTRTLSPQEFEEV
                     VAEVESLGFENAFIQSVPDSDAFMPDFRRNDPFRGNRR"
     gene            4156..5127
                     /gene="galE"
                     /locus_tag="PKK36_09805"
     CDS             4156..5127
                     /gene="galE"
                     /locus_tag="PKK36_09805"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNR94884.1"
                     /translation="MKILVTGGAGYIGSVTGRMLLDEGHEVVIFDSLERGHREAVDPR
                     AALIVGDLRNRQDIRAAMLKTKPDAVVHFAAFALVGESMEMPEIYFTNNVTGGIHLAD
                     AMLEAGVRKIIFSSTCATYGQPDVVPMTEDLPQRPTNPYGESKLMFEKVLLWYQQLHS
                     FEPVFLRYFNACGALGELGEDHDPETHLIPNILLVPLGKKEHVMMFGDDYDTPDGTCV
                     RDYIHIADLAQAHILALKKGVSGAFNLGNGDGYSVKQVIEAAREVTGHKIPAKMAPRR
                     PGDPARLVAAADKAKTILGWKPQYPDLRTIIEHAWQWHKSHPGGYRS"
     gene            5124..6350
                     /locus_tag="PKK36_09810"
     CDS             5124..6350
                     /locus_tag="PKK36_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNR94885.1"
                     /translation="MTRHVELISTGAELLIGRTVNSHAHALARALHPTGLRLSRDTTI
                     PDDPQVLLGTLKGALERVDVVVVTGGLGPTSDDITRETIAAWLDRPLVLDRDASERIR
                     QACEKRGLEPSQARMRQALVLEGAVVWPNLTGAAPGELIEENGKFIVLLPGPPREMEP
                     ILTQSLVPWIEQQYRLIPPPEHIFLLAGAAEGDIVTLFEKHHFPPDGIAAAYCASAGT
                     LEIRLSADSPVLLKGAADTVRGLLGDAVFAEERTSLEAVAGRMLAEKKLTLATAESCT
                     GGLLGSRITNVSGSSAYYLGGVVAYDNRIKIQLLGVSEDTLAGYGAVSPQTAKQMAEG
                     VRQRLGADFGIGITGIAGPAGGTAEKPVGLVYIALADDGQTKVRECRLSSNREFVRFM
                     STQLALDMLRLRLMQK"
     gene            6347..>6541
                     /locus_tag="PKK36_09815"
     CDS             6347..>6541
                     /locus_tag="PKK36_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNR94886.1"
                     /translation="MSAPNSIRAFIALKPDLDALRSIVKAQKSLRHCPAHVSWTHEDQ
                     IHLTLAFLGEISSATADALKP"
BASE COUNT         1498 a         1814 c         1948 g         1281 t
ORIGIN      
        1 gggcggccga gaaagcaggc tacgcaactg gaactcctct gtatgtgtac agcgaaaccg
       61 atcctgtcta tgcctcggag aaatcgggac tggccacggg aacccccgtc tatgcggaaa
      121 cagatccggt gtggattgcc gaaaaaggtg catacgcgac cggaactccg gtatatacag
      181 aaactgatcc ggtgtgggcc gccgagaaaa gcagctacgc cacgggaacc ccgctgtatg
      241 tttacacaga atcggatccc aatgccgtgc tggccgatgg ctcccgcgcg atggatggag
      301 atttggatat gggcggcaac agcatcacca acttttccac caacagcatt ttttttgccg
      361 acggaacggc aatgggtgtg acggatgtcc aaaactggaa cagcgcttat ggatggggag
      421 atcacagtgc ggcgggctac gccacaggaa ctccgctgta tgcccagtca gaaaccgatc
      481 cgctggccgt tctggcagac ggctcgcgcc ccatgtcggg ccgtctggac atgggaggca
      541 acgggatcac caatgtcaat ttcattgcct ttgatacgaa cgtccaggtc ggtgacggag
      601 cccagagttt cagctcgttg ggcggcagtg tggccattgg aaaggacgcc acggcgagat
      661 accgcacctc gggtctcgct atcggaacag aagcagacgg cgattacaac ggcatcgcca
      721 tcggatacca ggcggacggg cagtacagca acatcgcagt tggtttccag tccagcgcat
      781 attccggata tgatcggatt gcgatcggct gcaaaattac gaatcgggtg aacaattctt
      841 gtgcgatccg gggcacgctt tatctggacg ggggaacagg ggtcatggtc cgctcttctg
      901 ttggaacagg cgcatggtct gccaaagcat tcaccattga tcatccgctt gatcccgaaa
      961 acatggtgct ccggcacttc tgtctggagg gcccggaagt gctgaatgtc tacaacggca
     1021 atgcggtgct ggatgacgcg ggcgagacgg tgattacact gcccgactat tttgaggcga
     1081 tcaaccgcga cccgcgatac gcgctcactc ccattggcgc aaagatggat ctgtatatta
     1141 aacaagagat ttcagacaac cggttcattg tggccggcgg cgcgcccgga ggaaaggtca
     1201 gctgggaagt caaggcgctg cgttccgatc cggccctgct cgaagacatc aggcgccggc
     1261 cggtggagca gccgaaaagc gaactgacgc cggatcagat ggtccaggaa aatgagacgg
     1321 ttaacacctt cccatagcca tggagcctca tgttgagcca taaaaggttt gctccccgcg
     1381 tgcaactgcg gatacagtct tcttttgaaa tcagcccgtt aatccgtggt tcctgttcgg
     1441 aaatcacacc agctaaggaa ataagcaatg gataaccttg tacttgtcac cgtgaataat
     1501 gagagtttga ctcgcgaaga agcgcgccgg atcggccgcg acatggctgc gcggcagggc
     1561 attccccctc agatgattga caagtatctt gagcaggccg gagaagagct tgagaaacac
     1621 acggtcgagc agttcattaa ccaggcgctt atcaaggaag aggtggaccg ccgggcggtg
     1681 cccgtctctg aagaggaaat aggcgccgcc gtggcggaac tggaacgctc tttccccgag
     1741 ggcatgacac tcgacaaggt cctggtggcg cagggagtct cccgcaaggc ggtggaagag
     1801 gaaatcatcc gcaatgaacg cgtgcgcaag ctgtatgaaa cagaaaccgc cggaacgcct
     1861 tccgcgtccg atgaacaggt gagcgccttc tatcaggcca acagggagcg attcagaacg
     1921 gaagagtctg ccgaagtccg gcatattctg atcggatgca aggccggaga tgaccccgca
     1981 gttttccgga ccgcaaagga ggaagccgcc gcgattcggg agcagcttct caatggggcc
     2041 gattttggaa aactggcggc ggaaaaatcc gcctgcccca gcggcaagca gggcggaagc
     2101 ctgggcgacg tcaaaagggg acaaatggtg cccgcctttg aagaggcggt gttttcgcag
     2161 acggtcggag aaatcggtcc ggtggtgaca acggaattcg gctgccacgt cgtgcaagtg
     2221 accgcgcgga atgaagccgc cgtaataccc gaagaagatg cgaaagaaag cattcgcgaa
     2281 catctgtccg cacaggcaaa aggtctggcg tttgagaaat tcgtcgccgg tctgcgagga
     2341 aaagcccgca tcacttacgg cggccggggc ggcgggatca tcattccatg aaaaaccccg
     2401 gggaccgcgc cggaaagaca cagcaggaga tgatatcttg aaaacaatcc cgatcggcaa
     2461 agcattcacc ctgatggagc cgggtccggt ggttctggtt gtgacccgcg acgggaggaa
     2521 gaacaacatc atgacgatct cctggaccat ggtgatggac ttcactccga tacttgccat
     2581 caccacgggc ccatggaatt attctttcgc tgcacttcag aaaacaaaag agtgtgtgat
     2641 ttccattccg gcagtcgacc tcctcgacaa agtcgttgga gctggaacct gctccggcgc
     2701 cgacacagac aagtttgaaa aattcggcct gactcccgtg aagggaaaac atgtccgccc
     2761 acctctgatc agggagtgcg tggcaaacat cgaatgcaga gtgatcgaca tcgtaaaaaa
     2821 gcacaatatc gttgtgcttg aaggcgttgc ggcacacctc aacggatcac gaaaagagaa
     2881 gcgcaccatt catgctattg gcgacggcac atttgttgtt gacggacgga aactggatcg
     2941 gaggaagatg atggcctcca ggcttccata cgggatttaa ttcccggccc gaaccgggcg
     3001 ggatgcggaa caactggtat cccggacagg gatcgaacct gcgacctgcg gtttaggaaa
     3061 ccgccgctct gtccaactga gctaccggga cgccattaag tgattgagat aattcacact
     3121 tccgctattt tttcaacgca gaagatacac cctgaacatg aactgcgcca ctataaccaa
     3181 ttttgaggcc tatgaaaact gtcgcctgtg cccccgcgaa tgcggcgtgg accgggccgc
     3241 aggcaggacg ggtgtgtgcg aagaaacggc cgagtgccgc attgcctccg cgggaccgca
     3301 tttcggcgag gagccgtgct tcagcggaac acgcggctcg ggcacggtat ttttcaccgg
     3361 atgctccacg ggatgttttt tctgccagaa ctggcagatc tcccatgaag gcgcgggcca
     3421 cgtttatgac agggaatctt tttatgccct gctgaaggcg ctcgcagcca aagatgtgca
     3481 caacatcaac tttgtgacgc ccgaccactt ctggcctcac atcgcatggg cctgcagcag
     3541 gcttcgcacg gaaggcgttt ccatcccctt tctgttcaac agctccggct atcatcgcga
     3601 agacatgatt gaagcctatg ccggacacat gaatattttc atgccggact tcaagtttgc
     3661 cgatcccgaa ctggcacagc agtgcatgca ggaccggcgc tatccggata tcgccctcaa
     3721 agctatccgg aaaatggttg aactgaaggg ctttttgact ccgtgggacc cttcgggcga
     3781 aacgaccgcc gtatccggag tgcttgttcg ccacctggtg cttccgggtc atattcaaaa
     3841 cagcattgat gcgctgcatc tgcttaaaaa tgagttcggg cccctgcttc cgatttcagt
     3901 gatgagccag ttccggccaa cgcgtctctg cacggcgagg gattgcctga cgcgcacgct
     3961 ttcgccacag gagtttgaag aagttgtcgc ggaagtggaa tcactcggct ttgaaaatgc
     4021 atttattcag tctgttccgg acagcgacgc ctttatgccc gatttcaggc ggaacgaccc
     4081 gtttcggggc aaccgccgct gataccctca tgatttaact ggaacgcgga agctgcgcag
     4141 actagagttg tgtttatgaa gatactggtt acaggcggcg cgggttatat cggaagcgtc
     4201 acaggcagaa tgctgctgga tgaaggccac gaagtcgtca ttttcgacag ccttgagcgc
     4261 ggccaccgcg aggctgttga tccgcgcgcc gccctgatcg tcggcgacct gcgcaaccgg
     4321 caggacatcc gcgcggccat gctgaaaaca aagcccgacg ccgtagttca ttttgccgct
     4381 tttgcgctgg tcggcgaatc catggagatg ccggaaatct atttcacgaa caatgtaacc
     4441 gggggaattc atctggccga cgccatgctg gaggccggcg tcagaaaaat catcttctcc
     4501 tccacctgcg ccacctacgg gcagccggac gttgtgccca tgaccgagga tctgccgcag
     4561 agaccgacca atccctacgg cgagtcgaag ctgatgttcg aaaaggtgct gctgtggtat
     4621 cagcagcttc acagctttga acctgtgttt ctccgctatt tcaatgcctg cggcgccctg
     4681 ggcgaactgg gcgaggatca cgatcccgaa acacacctga tcccgaatat cctgctcgtt
     4741 cccctcggga aaaaagagca tgtgatgatg ttcggagacg attatgacac acccgacgga
     4801 acctgcgtgc gcgattacat tcatattgcc gatcttgctc aggctcatat tctggcgctg
     4861 aagaagggtg tttccggagc gttcaatctt ggcaacggag acgggtattc cgtgaaacag
     4921 gtgattgagg ccgcgcggga agtgaccggc cataagattc ccgcaaaaat ggccccgcgc
     4981 aggccgggcg atcccgcaag gctggtggcc gccgcggaca aggcgaaaac aattctgggc
     5041 tggaagccgc agtatccaga ccttcgcacg atcatcgagc atgcgtggca gtggcacaag
     5101 agccaccccg gcggttatcg ttcatgacgc gtcatgttga gctcatttcc accggcgccg
     5161 agctgctgat cggccgcacg gtcaacagtc acgcgcacgc actggcacgc gccctgcacc
     5221 cgaccgggct ccgcctgagc cgcgacacaa ccattcccga tgacccgcag gtgctgctgg
     5281 gaacgcttaa aggagccctg gaacgggtgg atgttgtagt tgtcaccgga ggactggggc
     5341 cgacttcgga cgatatcacc cgggaaacaa ttgccgcatg gctggaccgg ccgctggtgc
     5401 tggaccggga tgcgtcagag cgaatccgcc aggcatgtga aaagcggggt ttggaaccaa
     5461 gccaggcgcg catgcgtcag gctctggttt tggaaggagc cgtggtctgg ccgaacctca
     5521 ccggggccgc gccgggcgaa ctgattgagg aaaatggcaa atttattgtg cttctcccgg
     5581 gaccgccgcg cgagatggag ccgattctca cacagagcct tgtcccgtgg atcgaacagc
     5641 agtaccggct gattccgccg cctgagcata tatttctgct cgcgggcgcg gccgagggcg
     5701 atatcgtaac gctttttgaa aagcaccact ttccgcccga cgggattgcc gcagcctact
     5761 gcgcctcggc cggcacgctc gaaatccgcc tttctgctga ctcgccggtc ttgctgaaag
     5821 gggcggccga caccgtaaga ggactgctgg gggatgccgt gttcgccgaa gagcggacct
     5881 ctctggaggc ggtggccggc cggatgcttg ctgagaagaa actgactctc gcaacagcgg
     5941 aatcctgcac cggaggactt ctggggagcc gcattacgaa tgtcagcggc agctcggcat
     6001 attatctcgg cggcgtggtg gcttatgaca accggattaa aatccaactg ctcggagttt
     6061 ccgaagacac actggccggg tacggcgccg tgagccccca gacggcaaaa cagatggcgg
     6121 agggtgtcag acagcggctt ggagcggact tcggtattgg catcaccggc attgcgggac
     6181 cggcaggcgg aacggcggaa aaacccgtgg ggcttgtata tatcgccctt gccgatgacg
     6241 gacaaaccaa agtgagggag tgccggctga gctccaatcg cgaattcgtt cggttcatga
     6301 gcacacagct tgcactggat atgcttcgac ttcgtctgat gcagaaatga gcgctccgaa
     6361 ttccatacgg gcctttatcg ccctcaagcc cgaccttgat gccctgcggt caattgtaaa
     6421 ggcgcagaaa tcgcttcgtc actgccccgc ccatgtgagc tggacacatg aggatcagat
     6481 tcacctgacg ctggccttcc tgggggaaat ttcctctgcc acggcagatg cactgaagcc
     6541 g
//
LOCUS       DAOQWR010000135         6511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12238_length_6511_cov_4.001239, whole genome shotgun sequence.
ACCESSION   DAOQWR010000135 DAOQWR010000000
VERSION     DAOQWR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12238_length_6511_cov_4.001239"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..3536)
                     /locus_tag="PKK36_09820"
     CDS             complement(372..3536)
                     /locus_tag="PKK36_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR94887.1"
                     /translation="MDQRRCCTITAANLPASTYYTDRKGHRTKFAIGLGTAFPYQGRI
                     NSVEYADGTQAEYEYNAALQVTNVSDEAGQSWGYDYNEQGRLTDVLTPSGYQLSFAYT
                     NGGFDLARVTEAGSITLAAMTYTAQRDVATITNALNQAIRFGYDGFGRLTNAVDPLSL
                     ETVMEYGANGWVSEVKRDGISLATFQQDEMGRVTNSVGPENIPVAFSFDNLNRLSSLA
                     VSATEQPYSWTYQTNSLLLEKQTDRSGRRTSLEYDELERVEKVRGPDTSYVSFEYDSA
                     DNLSALLDGRGSRTRFSYDARDRLTQKTWPGGGGLSVAYDARGLPVSHTGGRGFVTLL
                     DYNGDGLLTNVAYTATNTPGVRYSYNSRNLLISSADGWSTNSYGYDLLGRLTNMVQIG
                     LAGTQQFFYAYDSLGQLTNLLWQCGTNSINTSYAYDAIGRVTNLVSDSGSYAYVYTNA
                     GLQVSRLTYPNGEYAASLYDSLGRLTNLAYSTGGNWTYAYDARDFVTRRVNPAGEVFT
                     YQYDDAGRLTDAIGLSGTNAISGYPLRYRYDKAGNRSSQTEGQKQRILAYNEDNQLLS
                     STRTNILTVRGYVNEPGSRIEARSNSNTNWIEGFVSFISYTQSYFEIHSVLTTNTYPS
                     SNTVWVRAYDVSNNVSTSVVHTASSSTIKREYSYDADGNLADFPAPVQFYMTNVLYSW
                     DAANRLTSIVCRTKPQVTNFFLVTTNKTCLKYDGMGRLRQISEYGTNARPTGVVRFVW
                     NGWTLAGELDGSNRLVRTYTYGVDLSGTVGGAAGIGGLVGIHSYAAPVTNYYTRHDGK
                     GNVTEVRRYNGTAAASYGYYEFGGVRYQTNTYMQNIRWQTRMMHPQSGLIYMIGRWYD
                     PVSGRFISIDPIQESGGWNLYQYAGGNPINNMDPDGKAIVPIVLTAAAIGTAVLFGYQ
                     IYKTVEGFKKLSELKAPEGGTMGNPAFEQFWINDVAPNVHQIVENCPNTMFTGPAAGG
                     QEGQAIDAIEDTYDFIQAERALTKPKRIRQVIGTARNLPDVQQVGNLNIPAGTYIRRN
                     QNNLEISTEQIRKVNKAIRRAVTE"
     gene            complement(4339..4653)
                     /locus_tag="PKK36_09825"
     CDS             complement(4339..4653)
                     /locus_tag="PKK36_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94888.1"
                     /translation="MEPFLIWLLSLFGLSLLLAMIGGSLHGMGVMRTQEIGLDDHEAN
                     IYFIIARLLKGVAIALGSISAALLFLWLVVSFADGDVNDESTTGFLTPSTGTEFRIIS
                     KE"
     gene            complement(4675..>6511)
                     /locus_tag="PKK36_09830"
     CDS             complement(4675..>6511)
                     /locus_tag="PKK36_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNR94889.1"
                     /translation="AYAYTNAGLQVSRLTYPNGEYAASLYDSLGRLTNLAYSTGGSWT
                     YAYDARDFVTRRVNPAGEVFTYIYDDAGRLTDAIGLSGTNAIGGYPLRYRYDKAGNRS
                     YQTEGQKQRILDFNEDNQLTLSDRDQTVAVRGTVNEPCSKIEVKSDSQSTWITAVVTM
                     VSYTQASFAAYGIAATNANYTGPTTNRIYVRATDMAGNISTSLVKIGYKPRDSVAYLY
                     DTDGNLLEFPVTPSTIQTNFVMSWDAENRLISMQRKYRISTVLQTNRSEFTYDSANRL
                     RQVKEYSTNGVLTNTTRYIWSGWQIAGELDGSNKLIRSYTHGVDISGTVGGAAGIGGL
                     VGIHSYVVPVTNYYIRHDGKGNVTEVRRYNGTTAASYGYYEFGGVRYQTNTYTQNIRW
                     QTRLMHSQSGLYFWPQRVYDPATGRWCQRDRIGEAGGANIYCYALQNPVNHFDALGYD
                     NQNINYRNVMGGSYNNSIPPGVDDVGRVVGGGQLQGVFYTTGIAAGTAAVGYGAGQLI
                     SGVFGYSTTALMEMAPAGQAHVISRIPVVWRPLIVLAEIIRVNAKIGTEIMYNHPEIP
                     IAAYIALEEYQLNSEIYDEIFGRIWESFFDNMKVDCQNDQSRDSK"
BASE COUNT         1592 a         1760 c         1546 g         1613 t
ORIGIN      
        1 ttctgccttt tctgaaaacc gccccgaaac ggggcggccc ctgcccgcgc gaagatctac
       61 tttgtgtctc tattcttgtt tccagtacct actaaaacaa gagatgctat tgctataaag
      121 cataaacata tagaaccgat tagaagtgag cggataataa gatgcgcttg ctgtatcaag
      181 accgttaaac ttgttgaggc atctatcaaa tccgcaggct tcatgctaga gagagaacca
      241 agcaacgcaa tatgttcacg aaatgtgcca attgtctgga aacgaaacaa taacagcgac
      301 atgattatca acgccaatat aaaaaacact aatgggattg tatttctttt catactattt
      361 cttcttcccc cttattctgt cactgctctg cgaatggctt tatttacttt acgtatctgc
      421 tcggtagata tttctaaatt attttgattg cggcgaatat atgtcccagc cgggatattc
      481 agattcccaa cctgctgaac gtcgggaaga ttccgggcag ttccgattac ttggcggatt
      541 cgctttggtt tagttaaggc gcgttccgct tggatgaaat catatgtatc ttctatcgcg
      601 tctatagcct gaccctcttg accaccagcc gctggcccag taaacatggt gttcgggcag
      661 ttttctacaa tttggtgaac atttggagca acatcgttga tccaaaattg ttcaaatgca
      721 ggatttccca tcgtcccacc ctcaggagcc ttaagctcag ataatttctt aaatccttct
      781 acagtcttat atatttgata gccaaaaaga accgcagtgc caattgctgc tgctgtcaac
      841 actattggta ctatggcttt gccatcgggg tccatattat ttataggatt gcccccagca
      901 tattgatata aattccaacc gccgctttcc tgaatgggat caatgctgat aaaacggccc
      961 gacaccggat cataccagcg gccaatcatg tatattaacc cgctctgcgg atgcatcatt
     1021 cgagtttgcc agcggatatt ctgcatgtag gtgttcgtct ggtatcgaac gccgccgaat
     1081 tcataatatc cataactggc cgctgcggtg ccgttgtatc ggcgaacttc ggtgacattc
     1141 cccttgccgt catgacgagt gtaataattc gtcacggggg cagcataact gtggataccc
     1201 accagcccgc caattcctgc cgcgccgcca actgtgccgg agagatcaac gccataagtg
     1261 tacgtccgca ccagcctatt gctgccgtcc agttcccctg ccagcgtcca tccattccac
     1321 acaaaacgca ccacgccggt gggccttgca tttgtgccgt attcgctgat ctggcgaagc
     1381 cttcccatac cgtcgtactt taagcaggtt ttattggtgg ttacgagaaa gaaattcgtg
     1441 acctgcggtt tggtgcggca cacaatggat gtgagccggt tcgctgcatc ccacgaatac
     1501 agaacattgg tcatgtagaa ctgaaccggt gccggaaagt ccgccagatt gccgtctgcg
     1561 tcatatgaat attcccgctt gatcgtggat gatgaagccg tgtgcacaac agacgtgctc
     1621 acgttgttgg acacatcata cgcccgcacc cagaccgtgt tgcttgaggg ataagtgttg
     1681 gtggtcaaca cactatggat ttcaaaatac gactgcgtgt agctaatgaa gctgacaaac
     1741 ccctcaatcc agtttgtgtt cgaattgctt cttgcttcaa tacggctgcc cggttcgttc
     1801 acgtatcccc gaacggtgag gatgttggtc cgcgttgagc ttagaagctg attgtcttcg
     1861 ttgtacgcaa gaatcctctg tttctggccc tctgtttgag aactgcgatt gcccgccttg
     1921 tcgtaccggt agcgaagcgg gtagccgctg atcgcgttgg ttccagataa tccaatggca
     1981 tccgtcagac gcccggcgtc atcgtactgg taagtgaata cctccccggc gggattcaca
     2041 cgccgggtca cgaaatcccg cgcatcatac gcataggtcc agtttccgcc ggtggaatat
     2101 gccagattcg tcaatcggcc cagtgaatca tacagcgaag cggcatattc cccgttggga
     2161 taggtcagcc gcgacacctg cagtcccgcg ttggtgtaga cataagcata agacccgctg
     2221 tcgctgacga gattcgtaac gcgcccgatc gcgtcataag catagctggt gttgattgaa
     2281 ttcgtaccgc actgccaaag cagattggtc agttgcccga gcgaatcata ggcatagaag
     2341 aactgctgcg tgcccgcgag gccaatttgc accatgttgg tcagtctgcc aagcagatca
     2401 tagccgtagc tgttcgtgga ccagccgtcc gccgacgaaa tcaggagatt tcgagagttg
     2461 taggaatatc ttactcccgg tgtattggtc gcagtatacg cgacgttggt caaaagcccg
     2521 tcgccgttat agtccagcag cgtcacaaaa ccgcgtccgc cggtgtgcga caccggcagg
     2581 ccccgcgcat catacgcgac ggagagcccg ccgccgcccg gccaggtctt ctgcgtcagc
     2641 cggtcgcggg catcataaga gaaccgcgtc cggctgcccc tcccgtccag cagggcggaa
     2701 aggttgtcgg cggaatcgta ttcgaatgac acgtatgagg tgtccggccc acgaactttt
     2761 tcgacgcgct caagttcatc gtattcaagg ctggttctgc gacccgagcg gtccgtctgc
     2821 ttttcaagca ataggctgtt ggtctggtag gtccaggaat atggctgttc cgttgcggaa
     2881 accgccagtg aggacagccg gttgagatta tcaaaactga acgcaaccgg gatgttttcc
     2941 ggcccgacgg agtttgtgac ccggcccatt tcatcctgct gaaaagtcgc cagtgaaatg
     3001 ccatcacgct ttacctcgga aacccagccg ttcgccccgt attccatgac cgtttcaagg
     3061 ctcaggggat ccaccgcatt cgtcagtctt ccgaatccgt cataaccgaa acgaatggcc
     3121 tgattcagcg cattggtgat cgttgccaca tcacgctgcg cggtgtaggt catcgcggcc
     3181 agcgtgatgc ttccggcttc cgtcacgcgg gcaaggtcaa agccgccgtt ggtgtaggca
     3241 aaggacagct gataaccgga gggagtcagc acatccgtta atcgtccctg ttcgttgtag
     3301 tcatagcccc atgactgtcc ggcctcgtcg gaaacgtttg tgacctgcaa agcggcgttg
     3361 tactcgtatt ccgcctgtgt gccgtccgca tattcgacgc tgttgattcg gccctgatac
     3421 ggaaaggctg tgccaaggcc aatcgcaaat ttcgtacggt gacccttgcg gtcggtataa
     3481 tatgttgaag cgggaagatt cgccgccgtt atagtacagc acctccgttg atccatccgc
     3541 ataagttgcg gtcagagtgc tccggtccca ggtgcccatt gcggacccgt ccgccagcgc
     3601 atagtcaaag gtcaccgaac cgcagtccgt gcgaagcgtc tggacgtaat cctgctgaag
     3661 tccattggtg taaaaagtgt aatgcgattc taaaacaaat gtgcgaaaga atctggacac
     3721 aaccccgcca cgctattcag ccaacgcagg cgtgaaacag tataccgcga gtgtcgccag
     3781 acaggcacag acaacaaata tataagaaga atattttgcg atcttttcac cccttcctgc
     3841 catcaagacg cggagaacaa atagacagca tacaaacacg gcaagccaaa caagctccgc
     3901 gaggttggcc agggtttttg agtgtccaag atatctgtgc acccttaagc cgtctaataa
     3961 ccagtataaa atcacgggga atatcaacat gataacatcc ggtaaatacc atttcttcac
     4021 agcgtatcgg cacctagcca ctaagaacac gagcgctgaa atggggagta ctagacaaaa
     4081 atacaatgta ttataagcca agaacgcttc catgaacttc ctccattatg gggttttaac
     4141 atcttcaaga tacatttctg gcaggggctt atttaaaccc ggattgaaac cgaaaggatc
     4201 aagttgctca acccaatcgc acgaaacacc tccgcctaaa agtgcacccg catgcttcca
     4261 cgccgtcttt caccgtccgg gacctaatct gatgcctata gacattcact tgtggcataa
     4321 cgatcgtacc tttctccgtt attcttttga aattatccga aattcggtcc cggtgcttgg
     4381 tgtaagaaac ccggttgtgg actcatcatt cacatcccca tcagcaaaag agacaaccag
     4441 ccacaggaaa aggagggcgg cagaaatcga accaagagca atagctaccc ctttcagcaa
     4501 tctggcaatt atgaagtaga tattagcctc atgatcatcc aacccaatct cttgtgttct
     4561 cattaccccc atcccatgaa gagatcctcc aatcattgcc agtaaaagag agagaccaaa
     4621 caacgacaac agccagataa gaaacggctc cattagttca cgtccttccg gggattattt
     4681 actatcacga ctctgatcgt tttgacaatc caccttcata ttgtcaaaga aactttccca
     4741 aatacgcccg aatatttcat catatatttc gctgttcagc tgatattcct caagcgcaat
     4801 atatgcagct attggaattt caggatgatt atacatgatt tctgtaccaa ttttagcatt
     4861 cacccgaata atttcagcca gcacaattaa tggtctccac actacgggaa tgcgagaaat
     4921 aacatgtgct tgtccagcgg gtgccatttc cataagagct gttgtagaat aaccaaatac
     4981 acccgagatc agttgccctg caccatagcc aactgctgcg gtgccagcag caattcctgt
     5041 agtatagaaa accccttgga gctgtccacc gccaaccacg cgaccgacat catccacgcc
     5101 tggaggaatc gaattattat aactgccgcc catgacattc cgatagttga tattttggtt
     5161 atcatatccc aaagcatcaa aatgatttac tggattttgt aatgcataac aatagatgtt
     5221 tgctcccccg gcttctccaa tcctgtcacg ctggcaccag cgccccgttg ccggatcata
     5281 aactcgctga ggccagaaat acaaaccgct ctgcgaatgc atcagccggg tttgccagcg
     5341 aatgttctgc gtgtaagtat tcgtctgata ccgaacaccg ccgaattcat aatatccata
     5401 gctggcggcg gttgttccgt tgtatctccg cacttcggtg acgttgccct tgccatcatg
     5461 acgaatgtaa taattcgtca cggggacaac ataactgtgg attcccacca gcccgccaat
     5521 acccgccgct ccgccaactg tgccggaaat gtcgacccca tgcgtgtaac tgcgaataag
     5581 tttattgctg ccgtccaatt cccccgcgat ctgccagcca gaccaaatgt atcgcgtggt
     5641 gttggtcaac actccattgg tgctgtattc ttttacctgc cgcaggcggt tggcgctatc
     5701 gtaggtaaat tcactccggt ttgtttgaag aacggtgctg attctgtatt tccgctgcat
     5761 ggaaataagc cggttctccg catcccaact cattacgaag ttggtctgaa tggtcgaagg
     5821 agtaaccggg aactcaagca ggttgccatc agtgtcataa agatatgcca cagaatcccg
     5881 cggcttgtat ccgattttaa ccagcgaggt gctaatgttt ccggccatgt ccgtggcgcg
     5941 gacatagatt ctgttggttg tcggtccggt gtagttggca ttcgtggcgg caatcccgta
     6001 cgccgcgaaa ctagcctgcg tgtaactgac cattgttaca accgccgtta tccaagtgct
     6061 ttgagagtcg cttttgacct caatcttgct gcacggttcg ttgacggttc cccgcacggc
     6121 aaccgtttga tcgcgatcag acagcgtcag ctgattatct tcgttgaaat ctagaattct
     6181 ctgcttctgt ccttcggttt gatagctgcg gttccccgcc ttgtcgtacc ggtagcgcag
     6241 cggatagccg ccgatggcat tggttcccga aagaccgatg gcatccgtca aacgtccggc
     6301 atcatcgtag atatacgtga aaacctcccc ggcgggattc acgcgccggg tcacgaaatc
     6361 ccgcgcatca taggcatagg tccagcttcc acccgtggaa tacgcaagat tggtcaatcg
     6421 gcccagtgaa tcatacagcg aagcagcata ttccccgttg ggataagtca gccgcgatac
     6481 ctgcaggccc gcgttggtgt aggcataagc a
//
LOCUS       DAOQWR010000136         6509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12245_length_6509_cov_4.777037, whole genome shotgun sequence.
ACCESSION   DAOQWR010000136 DAOQWR010000000
VERSION     DAOQWR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12245_length_6509_cov_4.777037"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKK36_09835"
     CDS             complement(<1..186)
                     /locus_tag="PKK36_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94890.1"
                     /translation="MMKWGAPEYLIWLWAMIPAAWFVFFMLRRREQRRAALISAQMLP
                     HLSPGFSAEVRRQKAACW"
     gene            complement(183..1169)
                     /locus_tag="PKK36_09840"
     CDS             complement(183..1169)
                     /locus_tag="PKK36_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR94891.1"
                     /translation="MILRHPLMLLCLLFIPAMVYLRHFRRRRPKILFSSGNLLGSLPA
                     SWAARASALLPFIYALGLVLLVIAMSRPQKGLDESVVRTDAVDIVLLVDVSTSMNAED
                     LATRMNRLDAAKAVIEKFIEARTRDRIGMVAFSAMPYTVAPLTLDHGWLLQRLRLLRT
                     GMIEDGTAIGDGLASAINRLRDSEAKSKVVILLTDGINNAGELTPENAAQAAKAVGIK
                     VYTIGAGSTSVVQVPFSGLFGGPRYVQQEAEIDEAALRMIAGTTGGMYSRARDIGELQ
                     KVYDQIDQMEKTEMEVEQYTRFEERFAGFALGGLLLLALEKLLALTRLGRYP"
     gene            complement(1166..2098)
                     /locus_tag="PKK36_09845"
     CDS             complement(1166..2098)
                     /locus_tag="PKK36_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94892.1"
                     /translation="MNRVFVIILLLHAAGCAQKPAPETNGGPFTLKMNLSTNVIHVGD
                     VVTMELNAVHPAETVLRIPEVGRGKELIVRRQGAETFRSGDKLKTFLRYELTSFEIGD
                     HLFPSGMVECVRNGEIISTLPFPAGQVTVESVLGPKDRELRDIEGLAAWPGIIPRWVW
                     ALLLVAGLAVAAALILGRFLSRPRTILQVPEGPPPHERALRAMARLREKDYIANENVE
                     PFYVELSAIIRTYLEDRFNLRAPERTTEEFIREAANSRLLSAPHQQLTRDFLEQSDLV
                     KFARHRPGRQEMLDGLAAGEALVKETAPREEVPA"
     gene            complement(2095..2979)
                     /locus_tag="PKK36_09850"
     CDS             complement(2095..2979)
                     /locus_tag="PKK36_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNR94893.1"
                     /translation="MIPREIIAKVRRIQIRSRHKVNDVFAGQYHSVFKGRGMEFQDVR
                     EYIPGDDIRSIDWNVTARTGHPFVKTFVEERELTVMLLVDISRSHVFGSGAQLKKDLA
                     AEIAAVLAFAAIRNNDRVGLILFSDEVECYIPPRKGTGHVFRVIREILYYKPNHPKTD
                     FQPALDYLNHISAHRTVSFLISDFIAEDICRKLLAVTARRHDLNAILVADKHEQSWPK
                     AGLVEWEDAETGRTFLVDTSNQAVRHALAARQAHRRAELLNELHGAGLDTIEVFAGEP
                     YERTLFNFFKLRERRLRT"
     gene            complement(2992..3981)
                     /locus_tag="PKK36_09855"
     CDS             complement(2992..3981)
                     /locus_tag="PKK36_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNR94894.1"
                     /translation="MSTGISLINEKVKEHNACIATLRAEMEKVIVGQKYLIDRLMVGL
                     LANGHILLEGVPGLAKTLSVKTLAAALSTTFRRIQFTPDLLPADLIGTLVYNPRDGSF
                     HTKKGPVFANIILADEINRAPAKVQSALLEAMQERQVTIGDETFRLSDPFLVMATENP
                     IEQEGTYPLPEAQVDRFMLKLKITYPSREEERRILEAMAVTSKSFTTKAVVTPAEIIA
                     AREVVDEIYVDDKIKDYILSIVFSTREPDQFKLDIGDFIRYGASPRASICLTVAARAH
                     AFLNGRGYVTPQDVKSIAPDVMRHRIIVSYEAEAEEMTADDLITRILNELPVP"
     gene            complement(4086..>6509)
                     /locus_tag="PKK36_09860"
     CDS             complement(4086..>6509)
                     /locus_tag="PKK36_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR94895.1"
                     /translation="NSNNKDHLQFAALAAHQLKSPVGAAATLLKTVLDEYAGELTPKQ
                     KDLLSKADARMEQALGSVRRILDIVQHEALSKKGEWSADLVAACHRVQLQHADAAAAR
                     QVTIQISSHTDRALVGIGEGSLTECIEALVSNAIKYTPEHGQIVLRIAAGQNDTDFFR
                     LSVEDSGIGIPYEDRKNLFTPFYRASSARDTAVAGVGLGLAFVKALVEAAGGTIRVDQ
                     SELGGAAFLLDLPRVYQQEKTAAETAAEDRKRKVVIIGGVAAGPKVASKVIRLDPMAE
                     VTVIERGTFLSYAGCGLPYYLSGIVKEQKELMASPLGMVRDPVFFQNVKRVTILSQTE
                     ATRIDRDKKAVEITDLASGRKSNIPYDTLVLTTGATPSIPPIPGSDLPNIFTLHGVRD
                     AEGIRHLLSAGKARDVVIVGGGLLGVEMTEALVARGCRVTITEKQPQILNILDWEMAR
                     AVDHHLESNGVRVLTSTLVQSFRGEGRVQEVYTSKGTIPADMVLLAMGVRPNVDLARE
                     AGLELGSTGAIRVSRRMQTSDPCIYAAGDCVETTDMVTGRPAYFPLGSTANKQGRAAA
                     VNICGGQDEFPGVLGSTICKVFDFCVARTGLTEDAAREAGFDAVSVLTPSPDREHFMP
                     GARMIMLKLVADRKSHRLLGAQCIGPGQGDKRIDVAATAILAGMTVEQVASLDLCYAP
                     PYAPAMDNIITAANVMKNKLAGHFEGISPREVKKKMDAKEDIVLLDVSTIREHEEMRI
                     PGTRVIPLGTLKSRINELPRDKEIIVFCTISLRGYEAVHILKAAGFRRVRVMDGGTVM
                     WPYEKVYGR"
BASE COUNT         1187 a         1946 c         2066 g         1310 t
ORIGIN      
        1 ccagcaggcc gctttttgcc tgcgcacttc ggcggaaaat cccgggctca gatgcggaag
       61 catctgcgcg gagatcagcg cggcccgccg ctgttcccgc cggcggagca tgaagaaaac
      121 gaaccatgcg gccgggatca tcgcccacag ccagatcaga tattctggag cgccccactt
      181 catcacgggt acctcccgag acgcgtcagc gcgagcagtt tttccagcgc cagcagaagg
      241 agccccccaa gcgcgaagcc ggcaaagcgt tcctcgaaac gcgtgtactg ctccacttcc
      301 atttccgtct tttccatctg atcaatctgg tcgtacacct tctgaagttc gccgatgtct
      361 cgggcgcggg aatacatgcc tccggtggtt ccggcgatca tgcgcagcgc ggcttcgtca
      421 atctccgctt cctgctgaac gtatcgcgga cctccgaaca ggccggagaa cggaacctga
      481 acaacgctcg tggatccggc tccgatggtg tagaccttta tgcccacagc ctttgcagcc
      541 tgtgccgcgt tttcgggagt cagttccccc gcgttgttta tcccgtcggt cagcaggata
      601 acaaccttgc ttttggcctc gctgtcgcgc aggcgattga tagcgctggc gagtccgtct
      661 ccgatggccg ttccatcctc tatcattccg gtgcggagga gccgcaggcg ctgaagcagc
      721 cagccgtgat caagggtgag cggcgcgacg gtgtagggca tggcggaaaa ggccaccatg
      781 ccaatccggt cgcgtgtgcg ggcctcgata aatttttcaa tgacggcttt ggccgcatcc
      841 agccggttca tccgggtggc cagatcctcg gcattcatgg aggtggaaac atccaccagc
      901 agcacaatgt ccacggcatc ggtgcggacg acgctttcat cgaggccttt ctgcgggcgg
      961 ctcatggcaa tgaccagcag aacgagcccc agcgcataga tgaagggaag gagcgccgag
     1021 gcgcgcgcgg cccagcttgc gggaagactg ccgagcagat tcccgctgct gaaaagaatt
     1081 ttcggccgac gccggcggaa atgccgaaga tagaccatgg ccgggatgaa cagcaggcag
     1141 agcagcatga ggggatgacg caggatcatg cgggcacctc ctcgcgcggg gcggtttcct
     1201 tcaccagggc ttctccggcg gcgagcccgt ccagcatttc ctgccgtccg ggccggtggc
     1261 gggcgaactt cacgaggtcg ctctgctcca gaaaatcgcg ggtgagctgc tgatgcgggg
     1321 cggaaagcag gcgcgagttg gccgcctcgc ggataaattc ctctgtggtc cgttcgggcg
     1381 cgcgcagatt gaagcggtct tcgagatagg tccggatgat ggcggacagc tcgacgtaaa
     1441 acggctccac gttttcattc gcaatgtagt ctttttcccg cagccgggcc atggcgcgga
     1501 gcgcccgttc atgcggcggc ggaccctccg ggacctgcag gatggtgcgg ggcctgctca
     1561 ggaagcggcc gaggatcagc gcggccgcga ccgccagtcc ggcaaccagc agcagcgccc
     1621 agacccagcg gggaatgatt cccggccatg cggccagccc ttcaatgtcg cgaagctccc
     1681 ggtccttcgg gccgagaaca ctttccacgg tgacttgtcc ggccgggaag gggagggtcg
     1741 aaatgatctc tccgttccgg acacactcca ccatgccaga cggaaacaga tgatcgccga
     1801 tttcaaagga ggtcagctca tagcgcagga aggtcttcag tttgtctccg gagcggaaag
     1861 tttcagcgcc ctgcctgcgg acgatcagtt ctttgccgcg gccgacctcg gggatgcgca
     1921 gaacagtttc tgccggatga accgcgttca gttccatggt gacgacatcg ccgacatgaa
     1981 tcacattggt gctgagattc attttgagcg tgaaggggcc gccgtttgtt tccggcgcag
     2041 gcttttgagc gcatcccgcc gcgtgcagaa gcagaataat gacaaagacc ctgttcatgt
     2101 ccgcagccgg cgctccctga gcttgaagaa gttgaagagc gtgcgctcgt aaggctcccc
     2161 ggcaaacacc tcgatggtgt ccagcccggc cccgtggagt tcattcagca gctccgcgcg
     2221 gcggtgtgcc tggcgggcgg ccagcgcgtg acgcaccgcc tggtttgaag tgtccacaag
     2281 gaaggtccgg cccgtctcgg catcctccca ttcgaccagt cccgctttgg gccaggactg
     2341 ctcgtgcttg tcggccacca ggatggcgtt cagatcgtgc cgccgcgcgg tgaccgccag
     2401 cagtttgcgg catatgtcct ccgcaatgaa gtcgctgatg aggaatgaaa ccgtgcggtg
     2461 cgcgctgatg tggttcaggt aatccagcgc gggctgaaaa tccgtcttgg gatggttcgg
     2521 cttgtaatag agaatttcgc ggatcacgcg gaacacgtgt ccggtgccct tgcggggcgg
     2581 gatgtaacat tccacttcat ccgaaaaaag gatcagcccg acacggtcgt tgttgcggat
     2641 ggcggcgaag gccagcacag ccgcgatttc cgcggcgagg tccttcttga gctgcgcacc
     2701 gcttccaaag acatgcgaac ggctgatgtc caccagaagc atcacggtca gttcgcgctc
     2761 ctccacgaag gttttgacga aggggtggcc ggtgcgtgcg gtcacgttcc agtcgatgga
     2821 acggatgtcg tcgccgggga tgtattcgcg gacatcctgg aattccatgc cgcgcccctt
     2881 gaacacgctg tgatactgtc cggcaaacac atcgttgacc ttgtgcctgc ttcggatctg
     2941 tatccgtcga accttcgcga tgatttctct gggaatcatg gcgcgcgctc ctcagggcac
     3001 gggcagctcg ttcagtatcc ttgtgatgag atcatccgcg gtcatttcct cggcctcggc
     3061 ctcgtaggaa acgatgatgc ggtggcgcat gacgtcgggg gcgatggact ttacatcctg
     3121 cggggtcaca taaccgcgac cgttcaggaa cgcatgggcg cgcgcggcca ccgtcaggca
     3181 gatgctggcg cgcggcgatg ccccgtagcg gatgaaatcg ccgatatcca gtttgaactg
     3241 atccggctcg cgcgtgctga acacgatgct gagaatgtag tcttttattt tgtcgtccac
     3301 atagatttca tccacgacct cgcgggcggc gatgatttcg gccggagtca cgacggcctt
     3361 tgttgtgaag ctcttgctgg tgacggccat ggcctcgaga atgcgccgtt cttcttcgcg
     3421 cgacggatag gtgattttca gcttgagcat gaagcggtcc acctgggcct cgggcagggg
     3481 ataggtgccc tcctgctcga tggggttttc cgtggccatc accagaaacg gatcggagag
     3541 cctgaaggtt tcatcgccaa tcgtgacctg gcgctcctgc atggcctcca gcagcgcgct
     3601 ctgcaccttg gccggcgcgc ggttgatttc gtcggcgaga atgatgttgg cgaaaaccgg
     3661 acctttcttc gtgtgaaacg agccgtcccg gggattgtaa acgagagtgc cgatgaggtc
     3721 ggcgggaagc aggtcgggag tgaactggat gcggcgaaag gtcgtgctga gcgccgcggc
     3781 gagggttttg accgacagcg ttttggccag ccccggcacg ccttcgagaa gaatgtgacc
     3841 gttggccagc agcccgacca tcaggcggtc aatcagatac ttctgcccga cgatcacctt
     3901 ttccatttcg gcgcggagcg tcgcgatgca ggcgttgtgc tccttcacct tttcattaat
     3961 cagactgatt cctgtgctca tattcctgat ctcctgatcg tttgaaaaac ttcatcactt
     4021 atgaccgctg aataaaaaac gagttcaaaa aaatatcaaa taaaatcaga atacgggggg
     4081 cgggatcagc ggccgtagac tttttcgtac ggccacatga cggtgccgcc gtccatgacc
     4141 ctgacgcggc ggaagccggc cgccttgagg atatggaccg cctcgtagcc ccggagggag
     4201 atggtgcaga acacaatgat ttccttgtcc cgcggaagct cgttgatccg gcttttcagt
     4261 gtgccgagcg ggatgacccg ggtgccgggg atgcgcattt cctcgtgctc cctgatcgtg
     4321 ctgacatcca gcagcacgat atcctctttg gcatccatct tctttttgac ctcgcgcggg
     4381 gaaattccct cgaagtgtcc ggccagcttg tttttcatga cattggccgc ggtgatgatg
     4441 ttgtccatgg ccggggcgta gggcggggca tagcagaggt ccaggctggc gacctgctca
     4501 acggtcatgc cggccaggat ggcggtcgcg gccacgtcta tccgcttgtc accctgcccg
     4561 gggccgatgc actgcgcgcc gagaagccgg tgggacttcc ggtcggccac gagcttcagc
     4621 atgatcattc tggcgccggg catgaagtgc tcccggtccg gagacggggt cagcacactg
     4681 acggcatcaa agccggcctc gcgcgcggcg tcttccgtga ggccggtgcg ggccacgcag
     4741 aaatcaaaga ccttgcagat ggtgcttccg aggacgccgg ggaattcatc ctgtccgccg
     4801 cagatgttca cagcggcggc gcggccctgc ttgttggcgg tggagccgag cgggaaatag
     4861 gcgggccggc cggtgaccat gtccgtggtt tcgacgcagt ctccggcggc atagatgcag
     4921 ggatcggaag tctgcatccg gcggctgacc cggattgcgc cggtggaacc gagttcgagc
     4981 cctgcttcgc gcgcaaggtc cacgttgggc cggacgccca tggccaggag gaccatgtcg
     5041 gcgggaatgg tgcctttgct cgtgtacacc tcctgcaccc ggccctcgcc ccggaaggac
     5101 tgcacaaggg tgctggtcag cacccggacg ccgttggatt ccagatggtg atccacggcc
     5161 cgcgccattt cccagtccag gatgttcaga atctggggct gcttttccgt gatggtcaca
     5221 cggcatccgc gcgcgaccag cgcttcggtc atttcaactc ccagcagtcc gccgccgaca
     5281 atcacgacat cgcgggcctt gccggccgaa agcaggtgcc ggatgccctc cgcatcgcgc
     5341 acgccgtgaa gcgtgaagat gttgggcagg tcggaacccg gaatcggagg gatgctgggt
     5401 gtggcgccgg tggtgagaac cagcgtgtca taggggatat tcgatttccg gccgctggcc
     5461 agatccgtga tttcaacggc ctttttatcg cggtcaatgc gggtggcttc agtctggctg
     5521 aggatggtga cccgcttgac attctggaag aaaaccggat cgcgcaccat gcccagcgga
     5581 ctggccatga gctctttctg ctccttgacg atgccggaaa gatagtaggg caggccgcag
     5641 cccgcatacg aaaggaacgt gccgcgctcg atgacggtca cctccgccat gggatccagc
     5701 cgtatgacct tggaagccac cttggggccg gcggcaactc cgccgatgat caccactttc
     5761 ctttttctgt cttccgcggc ggtttcggcc gcggtttttt cctgctggta cacgcgcggg
     5821 agatccagca ggaaggcggc tccgccgagt tccgactgat ccacgcggat ggttcccccg
     5881 gccgcttcga ccagcgcctt cacaaaggcc agtccgagcc cgacccccgc cacggcggtg
     5941 tcgcgcgcgg aactggcgcg atagaaggga gtaaaaagat tcttgcggtc ttcgtagggt
     6001 attccgatgc cggagtcctc gaccgacagc cggaaaaagt ccgtgtcatt ctgcccggcg
     6061 gcaatgcgga gcacgatctg gccgtgctcg ggcgtgtatt tgatcgcatt gctcacgagg
     6121 gcctcgatgc attcggtcag cgatccttcg ccgatgccga cgagcgccct gtcggtatgg
     6181 gatgaaatct ggatggtgac ctgccgggcg gcggcggcat cggcatgctg gagctggacg
     6241 cgatgacagg cggccaccag atcggccgac cattccccct tcttggagag cgcctcgtgc
     6301 tgaacaatgt cgaggatgcg ccgcacgctg cccagcgcct gttccatgcg cgcatcggcc
     6361 ttgctgagca ggtccttctg cttcggggtc agctcgccgg cgtattcatc cagaaccgtt
     6421 ttcagaagcg tggcggcggc tccgaccggg gatttgagct gatgcgcggc cagcgccgcg
     6481 aactgcagat gatccttgtt gttcgaatt
//
LOCUS       DAOQWR010000137         6449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12381_length_6449_cov_5.755865, whole genome shotgun sequence.
ACCESSION   DAOQWR010000137 DAOQWR010000000
VERSION     DAOQWR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_6449_cov_5.755865"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..81)
                     /locus_tag="PKK36_09865"
     tRNA            complement(5..81)
                     /locus_tag="PKK36_09865"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Pro,seq:tgg)
     gene            203..1384
                     /locus_tag="PKK36_09870"
     CDS             203..1384
                     /locus_tag="PKK36_09870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR94896.1"
                     /translation="MSSEISHYATTKVEILGSETLVDISSPQRPLEPSARDLYRNYKA
                     IIRDRNIYYPVAYRLVRELGHGRQGRVFLGLRQGARGCITRHAIKIFDPSIYTSAENY
                     WTDMGRIANQVSELQMVQSPNLTSRDIYEEFNGIGYMQMQVIDGVDLRELLSPGHLDI
                     VKARSTPEEWARFMDVIFRVEGNTLKIQTGIALYIMRQMLRGLETLHEHGFVHSDIKP
                     ANIMIDRLGYVKLVDYGRAVKANEKMTMLFGSPLFMAPETHRREPNVIESDIYSVGLV
                     GLEMLRGEPLLDTTGLLEKDLLEFKMSLPDRLQDMLPSYVLQNSQLVRMFRRFIDPDP
                     QKRYHKADEAEAGKQGLRIVHQQLTQLGKDAEYGRELQAYISKIKAAPADSFSRDIER
                     L"
     gene            1451..2098
                     /locus_tag="PKK36_09875"
     CDS             1451..2098
                     /locus_tag="PKK36_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNR94897.1"
                     /translation="MNILHICANPKPTEESVSKQLAASFFITLANEFPDYTVENVDLY
                     QSPPPYISYDAWRGFYLPVYVNGYHATDKEVAATNYARKQGKLFNAADVLVLTTPMWN
                     YNMPAILKAWFDQVLSPGITYEVEKTGPKPLHSIKKVVLLVASGGTYKEDDPADALGR
                     GVTAALKFIGITDISVAWADGQNPLYFDNSETRKQMALEAVQEIAEEVAEMAAEF"
     gene            2107..2589
                     /gene="tadA"
                     /locus_tag="PKK36_09880"
     CDS             2107..2589
                     /gene="tadA"
                     /locus_tag="PKK36_09880"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNR94898.1"
                     /translation="MDDIRQELNPDILYMRMALRQAEAAAEAGEVPVGAVIVHNGEVI
                     GRAHNQVELLKDPTAHAEILAITQASQALGDWRLLDTVLYVTKEPCPMCAGAIMLARI
                     PKVIWGVDDPQRGGAVSVFSILNHKNLNHRAECVRGLMEDECRAVIQGFFRSRRQESE
                     "
     gene            complement(3455..3850)
                     /locus_tag="PKK36_09885"
     CDS             complement(3455..3850)
                     /locus_tag="PKK36_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNR94899.1"
                     /translation="MKRSIDGEMVKGYSELIVLALIKRLGPVHGYALRAEMRDLSYQI
                     FHLSWGRIYPLLHDLEKRHLLTSRIQPAGKLRTQRRFSITKMGEEYLQHRIQKWEMFS
                     MALNAAIQQKGNRKQRAKEQLRRLPADVR"
     gene            complement(4095..5114)
                     /locus_tag="PKK36_09890"
     CDS             complement(4095..5114)
                     /locus_tag="PKK36_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNR94900.1"
                     /translation="MKWIRIPALMIMAGLTAVPTLEAATSRGQIIGDNVNLRAKDQPE
                     AEVVTQLNDGDAVEIKGYSGDWAEVVPPEKADLWVHKDFVQDGVVRGKTLNVRAGPGI
                     NFNVVGTLSRGDKVTVRGEMTEWYKIAPPAACSLWVSRQYVGSPSKPKPEPERPRPVV
                     PPARQTATVPAPVRRPDDARVEQPAPQRQTVTIPPRVVAAAPAAEGVQSDLPANWKLI
                     PLEGQGKRIERSGRLRSVGWRLRRPSHFRLVRYLDSGRFETICYIRGNREQLSEWVDE
                     EIGIRGREYWIQNEKYPIVVVDQLMLPARTRQVAPVQQQAVPPIRPRPLPPSEMPDGE
                     FNPTN"
     gene            5302..>6449
                     /locus_tag="PKK36_09895"
     CDS             5302..>6449
                     /locus_tag="PKK36_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94901.1"
                     /translation="MSPEVRALLVLLISTLLVSHAQSEDIRAVLDSTDGSTAFVVTDS
                     ETNEQLRAGSDGVVSVNGTLSVSETQNRGSLDRAMLIIGGGDDIEIGAGANGYLTGVA
                     VGKNANGYLGGAVVGAGAYGVAYGAAVGFNANALSDGAAAGRSANGSHAGVAVGAFSS
                     GYAAGIAIGNSACGSQTNIAIGNMASSGVGPERIAIGHMVATPQDNAAAIRGTLFLDG
                     GTNVMMRSTFGSGAWVPLSSLAGDFKANGSVPMSGNLNLAGQVLTNVALVDFNSSSTA
                     VGRGADAGLYGTALGYGTVATNRGTAVGYISSAYGYGAAVGRESRGYSYGAAIGENAW
                     GYENGVAVGYWSDGYAGGVALGYHANGAKTNIAIGGYAHAYGTDRIAIG"
BASE COUNT         1341 a         1942 c         1867 g         1299 t
ORIGIN      
        1 agtatggtcg gggcggtggg atttgaaccc acgacttaca gctcccaaag ctgtcgcgct
       61 accagtctgc gccacgcccc gccgaggaag ttagaccgtc aatatgccag aatcccggcc
      121 gttttccaca agaatttcca gccgggcaaa tatttaacgt tccttattgc caaatggagc
      181 ccgattcgca atcttctccg ccatgtcatc ggaaatcagt cattacgcca ccacgaaggt
      241 ggagatactg ggctcggaga cgctggtgga catcagcagt ccgcagcggc cactggagcc
      301 cagcgcacgg gatctgtacc ggaactacaa ggcgatcatc cgcgaccgga atatctatta
      361 tcccgtggcc taccggctgg tccgtgaact cggccacggc cggcaggggc gcgttttcct
      421 cggtctccgg cagggtgccc gcggatgcat cacccgtcac gcaatcaaga tatttgaccc
      481 gagcatctac acgtcagccg agaactactg gacggacatg ggccgtatcg ccaaccaggt
      541 gtcggaactg cagatggtgc agagtccgaa cctgacctcc cgcgacatct acgaggagtt
      601 caacggcatc ggctacatgc agatgcaggt gattgacggc gtggatctgc gcgagctgct
      661 ttcgcccggc cacctggaca tcgtgaaggc gcgttccacg cccgaggagt gggcccggtt
      721 catggatgtg atcttccgcg tggagggaaa caccctcaag attcagaccg gcattgcgct
      781 ctatatcatg cgccagatgc tgcgcgggct cgaaaccctg cacgagcacg gctttgtgca
      841 cagcgacatc aagcccgcca acatcatgat tgaccggctg ggctatgtga agctggtgga
      901 ctacggccgg gcggtcaagg ccaatgaaaa aatgacgatg ctgttcggaa gcccgctttt
      961 catggcgccc gaaacccacc gccgcgagcc gaacgtgatc gaatccgaca tctacagcgt
     1021 cggtctggtg ggactggaga tgctgcgcgg ggagcccctc ctggacacca cggggcttct
     1081 ggaaaaagat ctgctggagt tcaagatgtc cctgcccgac cgcctgcagg acatgctccc
     1141 gagctatgtg ctgcagaact cgcagctcgt gcgcatgttc cggcgcttca ttgatccgga
     1201 tccgcagaag cgctatcaca aggcggacga ggccgaagcc ggcaagcagg gcctgcgcat
     1261 cgtgcaccag cagctcaccc agctcgggaa ggatgccgag tacgggcgcg agcttcaggc
     1321 ctacatctcg aagatcaagg ccgcgccggc cgattcattt tcacgggaca ttgagagact
     1381 ttaggctttt gctcgaacgc gatatcccct atgatgcgca acttgaattt ttgaaacggt
     1441 aaggagaagt atgaacatat tgcacatttg cgcgaacccg aaaccgaccg aagagtcggt
     1501 ctcgaagcag ctggcggcgt cttttttcat cacgctggcc aatgagtttc ccgactatac
     1561 agtggagaat gtggacctct atcagtcccc gcccccgtat atatcctacg acgcgtggcg
     1621 cggtttctat ctgccggtgt atgtgaacgg ctatcacgcc acggacaagg aagtggcggc
     1681 caccaactac gcccgcaagc agggcaagct cttcaacgcg gccgatgtgc tcgtgctgac
     1741 caccccgatg tggaactaca acatgcccgc catcctgaag gcgtggttcg accaggtgct
     1801 gtctcccggc atcacctacg aggtggagaa aaccggcccc aagccgctgc acagcatcaa
     1861 gaaggtcgtg cttctggtgg cttccggcgg aacctacaag gaagatgatc cggccgacgc
     1921 cctcggccgc ggcgtgaccg ccgccctcaa gttcatcggc atcaccgata tttccgtggc
     1981 gtgggccgac ggccagaatc cgctgtactt cgacaactcg gagacgcgca agcagatggc
     2041 gctcgaggcc gtgcaggaaa tcgccgagga agtggccgag atggccgccg agttctaagc
     2101 ggattcatgg acgacatccg gcaggagctg aatcccgaca ttctttacat gcgcatggcg
     2161 ctgcggcagg ccgaggccgc cgcagaggcc ggcgaagttc cggtcggcgc cgtgatcgtg
     2221 cacaacggcg aagtgatcgg gcgggcgcac aaccaggttg aactgctcaa ggatcccacc
     2281 gcgcatgccg agatactcgc catcacgcag gcctcgcagg cgctgggcga ctggcggctg
     2341 ctggacaccg ttctctatgt caccaaggag ccctgcccga tgtgcgcggg cgccatcatg
     2401 ctcgcgcgca tcccgaaagt gatctggggg gtggacgatc cgcagcgcgg cggcgcggtc
     2461 tccgttttca gcatcctcaa ccacaagaat ctgaaccacc gggccgagtg cgtgcgcgga
     2521 ctcatggaag acgaatgccg agccgtgatc cagggtttct tccggagccg gcggcaggaa
     2581 tctgaatagc cgaaaatccg gaatccgttt caattcccga catgccgatc aggagatcgg
     2641 cgctcccagg gctcccggga acgccgcgca ccagcgcgga aaatgcggaa ccgacggagc
     2701 ggtaccctcc attgagaatc acaaaacagc cctgtgggcg tggcgacgcc tctgtgaggc
     2761 gcgattgtgt ttagtaacag caccccacgc atccccatgc gccaggatat tcccgtcaat
     2821 atatcacaaa gtgatatatt tccgagccac cgcactttcg cgagtttcgc gtcttttgca
     2881 gtttcaattc tgaacgcgcc gctcacaggg cctctacatc acagagcaag cctgtgggtg
     2941 tggcggcgcc tctgtgaggc gcgattgtgt ttagtaacag caccccacgc atccccatgc
     3001 gccaggatat tcccgtcata tatcacatag tgatatattc ccgagccacc gcactttcgc
     3061 gcttttcgcg tcattcgtag tctcactttc gaacgtgccg atctggaaat cggcgatccc
     3121 agggctcaca ggaacgccgc gctccagtgc ggcatatgct gaactgacgg tgtactttcc
     3181 agcatcattg cgagacaaga tccagtgaat tccaggtcat catcgacaat atatcactat
     3241 gtgatatatt gtcgtcatag cagatgatgg aggggtttgt tctgtcaaaa agccacggcg
     3301 agcgctgtgg tgcaaaccct cctgtgctgc tcatcagcgt ttcgctccga tgccctcccc
     3361 tcgcaaggtc atcatcgtca atatatcaca cagtgatata tcatcgcacc gcggtttatg
     3421 agattgaacg caagtgcgat agatgcaaaa gggattaacg aacatctgcc ggcagccggc
     3481 gcaactgttc ctttgcgcgc tgtttccggt tccccttttg ttggattgcc gcattcaatg
     3541 ccatcgagaa catctcccat ttctgtatgc ggtgctggag atattcctct cccatctttg
     3601 tgatgctaaa ccggcgctgc gtgcgcagtt tgcccgccgg ctgaatgcgg ctcgtcagca
     3661 gatgcctttt ttcgagatcg tggagcagcg ggtatatgcg cccccatgac aggtggaata
     3721 tctggtagga caaatcccgc atctccgccc gcagcgcgta cccgtgcacc ggccccaggc
     3781 gcttgatcag cgccagcaca atcagttccg aataaccctt caccatttcg ccgtcaatgc
     3841 tccgtttcat gggcgtcaat atatcattat gtgatatatt gacaagccga aaagtcaatg
     3901 ctcagcatgt gtgctgaaga ggcggacgcg aggaggcggt gtgcggcaaa ggcacggatt
     3961 cacgggcccg ttccggcgcg gttttgattt ccaatcactg gcagttggag gctggagaaa
     4021 ataaaacgcc attttggcag cataccgccg gcaaccaccc gcatcgcttt caatcccgac
     4081 ctgaatcacg caaactaatt cgtcgggttg aactctccat cgggcatttc ggaaggcggc
     4141 agcgggcggg ggcggatcgg gggaaccgcc tgctgctgga ccggcgcgac ttgacgcgtg
     4201 cgcgccggaa gcatgagctg gtccaccacc acaatcggat atttctcgtt ctgaatccag
     4261 tattcgcggc cgcgaatgcc gatctcctca tccacccatt cggagagctg ctcgcggttg
     4321 ccgcgaatat agcagatggt ctcaaagcgg ccggaatcca gatagcgcac caggcggaaa
     4381 tgcgacgggc ggcgcagcct ccagcccacg cttctcagcc ggcccgaacg ttcaatgcgc
     4441 ttcccctgcc cctcaagcgg tatcagcttc cagttcgcag gcagatcgga ctgcactcct
     4501 tcggccgccg gcgcagccgc cacaacccgc ggcggtatgg tgacggtctg gcgctgcggc
     4561 gcgggctgtt ccactcgcgc atcatccggc ctgcggacag gcgcgggcac cgtggccgtc
     4621 tgccgcgcgg gcggcaccac cgggcgagga cgctccggtt ccggtttcgg ctttgacgga
     4681 ctgcccacat actgccggct gacccacagc gaacaggctg cgggaggcgc aatcttgtac
     4741 cattccgtca tctcgccgcg gacggtcacc ttgtcgcccc gcgaaagcgt gcccaccaca
     4801 ttgaagttga tgccgggacc ggcccgcaca ttgagcgtct tgccgcgcac aacgccgtcc
     4861 tggacaaaat ccttgtgcac ccagagatcg gctttttcgg gcggaacgac ctccgcccag
     4921 tcgccggaat accccttgat ctcgacggcg tcgccgtcgt tcagctgggt caccacttcg
     4981 gcctcgggct ggtccttcgc ccgcagattc acattgtccc cgatgatctg cccgcgggag
     5041 gtcgccgcct ccagcgtcgg aacagccgtc agccccgcca tgatcatcag cgccggaatt
     5101 cgaatccatt tcataacaca cagctccagt taaataccgt gttcagttta gctgtccgac
     5161 cgcgcaaggg aaagcaattt tccagtcatt ggaagttttc atctgtcaaa tccccttttt
     5221 cttgtacttt actgattcct gatcataagt tacaaacgct ttcgatgaga ctggttgagg
     5281 aaccgttaaa cggaggagat catgagtcct gaagtgcgcg cattgcttgt tcttcttata
     5341 tccaccctgc ttgtcagcca tgcacaatcg gaggacatcc gggccgtgct cgattcgaca
     5401 gacggcagca cggcctttgt tgtaaccgac tccgaaacga acgaacagct tcgtgcgggt
     5461 tcagatggcg tggtttccgt caacggcaca ctgtcggtca gtgaaacaca gaatcgaggt
     5521 tcgcttgatc gggccatgct tattatcggt ggtggagatg atatcgaaat cggggccggt
     5581 gcaaatggat acctgacggg agttgctgtc gggaaaaacg ccaatggtta tttaggcgga
     5641 gctgtcgttg gtgcaggcgc atacggagtg gcctatggtg cagccgtcgg cttcaacgca
     5701 aacgcactca gtgatggtgc tgccgccggc cgcagcgcaa acgggtctca tgccggtgtt
     5761 gcagtcggcg cgttctcttc tggctacgcg gcaggcattg ccatcgggaa ttccgcctgc
     5821 ggatcgcaga ccaacatagc cataggcaat atggcttcct cgggcgtggg cccggaacgc
     5881 attgccatcg gccacatggt cgccacccct caagacaacg ccgcagccat ccgcggaacg
     5941 ctcttccttg acggcggaac caacgtgatg atgcgctcca ctttcgggtc cggcgcatgg
     6001 gtgccgctgt cttcgctggc gggcgacttc aaggccaatg gttccgtgcc catgtcgggt
     6061 aatctgaacc tggccggaca ggtactgaca aatgtggcac tcgtggattt taacagctcc
     6121 agcaccgcag ttggacgcgg tgctgatgcg ggtttatacg gaacggcttt gggctatggc
     6181 accgtggcaa ccaaccgcgg tacggcagtg ggatatatat ccagcgcgta cggctatgga
     6241 gctgcggtcg gccgcgaatc cagaggctac agctacggcg cggccattgg agagaatgca
     6301 tggggttatg aaaatggcgt tgcggttgga tattggtccg acggctacgc ggggggagtc
     6361 gctttgggct accatgccaa tggagcgaaa accaacatcg ccatcggagg ctacgcccac
     6421 gcctacggga cagaccgtat cgcaatcgg
//
LOCUS       DAOQWR010000138         6389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12533_length_6389_cov_4.935112, whole genome shotgun sequence.
ACCESSION   DAOQWR010000138 DAOQWR010000000
VERSION     DAOQWR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_6389_cov_4.935112"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PKK36_09900"
     CDS             <1..1369
                     /locus_tag="PKK36_09900"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNR94902.1"
                     /translation="LDASASAPPAAIGAAAALLRPQVIKGANPSLMYIVTNLPLGEIE
                     FPNMNLKWASTRPDIIDEQGRVSRPVGSEPVAVELSLHLKSDGAETSRVYKAAVQPQK
                     KSLPLVQVKAPDFPNNMYRIPCVGGISGHEEILPESPGEIRIRGNSAIRYPKKSYSIA
                     LDRPHGVFGDTDSRHLYLVGCGKDPSLIRNKLAYELFRDFGSEAAPRIIPRVEMVELI
                     LNGEYLGIYQLTQRVDRHMLGWKAFNSCEQHHNVLYKADGPGAGFLEVHQDQYEQKEP
                     DEVQCPCWEPYSEIIRFIAESSPEEFRAHVAEHMDIANIVDFHLFLLLTNNRDGNNHN
                     LYLAREGTTGSRFFIIPWDYDTAFMDDGFLSNGLFERLEADLPGYPGMLKERWVQLRQ
                     GICDKQAIFDRIDAMHAENAPAVEREKIGEVPGAKRRDAAVRELKAWISARLLEVDRH
                     LASMP"
     gene            complement(1491..1697)
                     /gene="tatA"
                     /locus_tag="PKK36_09905"
     CDS             complement(1491..1697)
                     /gene="tatA"
                     /locus_tag="PKK36_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNR94903.1"
                     /translation="MNTLAFFSGRPGAGEVILILFIVLLLFGAKRLPDLARSLGKSLS
                     EFKKGKDEAMKEIAGDKPEDKKPE"
     gene            complement(1705..2445)
                     /gene="tatC"
                     /locus_tag="PKK36_09910"
     CDS             complement(1705..2445)
                     /gene="tatC"
                     /locus_tag="PKK36_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNR94904.1"
                     /translation="MNSGVPEPGRTMPFLQHLEELRRTILWCLGVFTGGLFITFPLAP
                     AILNLLREPLARTVINPGQFLQSIEVGAAFSVSLRIAGWGSLLITSPLMLFFITRFVF
                     PGLKDAERSAIKKAFCFSLALFVSGVVIAWWTTLPAALQMMMRMHDWMGITAIWTVSS
                     YVRFCLGLLIGFGLAFQLPVIVFVLGSLGIVNAKMLAEKRRHVIVGLLVLAMLLTPPD
                     VITQLLMAIPLILLYEVCIIILKVKQKP"
     gene            complement(2442..3452)
                     /locus_tag="PKK36_09915"
     CDS             complement(2442..3452)
                     /locus_tag="PKK36_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94905.1"
                     /translation="MNKAKKILLLAVLTPVTAFSQPPQVPIAPRAGGEYMGWTNSFRL
                     ANGLVDAVIVPEAGRMIHFGPAGGENLLRLDKSLAGKSYPRKNGKDWKNFGGQWMWPA
                     AQSAWPSFQTNDWPPSSVIDRKPWTGFAWTEADGAAACVITRHFGEPLHVEARQLYRL
                     MPSNDFITIRQSLVRTEKSSVPVTIWNLVQVPLPERIAFETGLEQEGSPGWKTLKFSP
                     PDPEQIQTCGSSVVYEPGTSEETKIGSRAPVARMAAFKGPQAVIMKAAANSGEPDGKI
                     CTEIYVNSGLGYAELEILSTETNLVEDEAISFTTQIQVLNIIPAAKDPCGQLAETKAL
                     SP"
     gene            3565..4656
                     /locus_tag="PKK36_09920"
     CDS             3565..4656
                     /locus_tag="PKK36_09920"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HNR94906.1"
                     /translation="MRTELFFRLGGEGANVSGQEWDDFMAQYVTPRFEQHVQIAEVTE
                     HFLGDKGRELIPVKVLTCLHDSTPAADESIDYIRASYVRLFRKKQVVRISVPVAASFR
                     QDMKEPDWRELGTLQNAEIRESSGMAYSTRGHSVLWTHNDSGDTSRFFAVGLAGEDLG
                     TFMLEGAKAVDWEDIASFTFDGKSWLLLADVGDNEERRPDCQLYIVEEPQAGTSATVS
                     VERMISFVYEDGPHNCEAVAVDPEEGRILLIVKTRDPASGVYSLPLNPEDEKTTARRI
                     ADIRMTGVVAMDISPDGRDAVVLTYGDAFRWRRAEDEGWASAFGREPEMLPMPYRKQG
                     EAVCFTPDGKKLLLSSEKKPRPLLEVTLP"
     gene            4858..6003
                     /locus_tag="PKK36_09925"
     CDS             4858..6003
                     /locus_tag="PKK36_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR94907.1"
                     /translation="MASIHRRKGTKKWHAAFYDAEGRRCMRSTGTSKRGDAMSIALDW
                     EEAARRGREGRLTVDQARKVIADIYARANRDKLPAGTVREYVKHWMQMKEAGLADSSF
                     TEYKRLTADLIDFLGKRADGPLDAITSKDIVKYRARLLARVSPATVNKSLKALRGIWT
                     QARKDELITRNVFDSVSYVKAEQKKTRRKNFTGEQVKALLPYCKDSWRGLVLAGLYTG
                     QRLSDLLALTWEEIDLAGAKIQFTTQKTGAELEIPIHPALMKWLMDNAGDDPAALVFP
                     DFAGISQNTVSRQFMKKLHNAGLIKAEPSHKSANKGRDSKRTLNELSFHSLRHAYVTM
                     MKEAGATDGITKSIVGHQSDAVNRLYTHIGIDAMREAVNRLPDVTAG"
     gene            complement(6000..>6389)
                     /locus_tag="PKK36_09930"
     CDS             complement(6000..>6389)
                     /locus_tag="PKK36_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94908.1"
                     /translation="ARGKSPLPAMVLFWNSVPALVFARSPEEKAIISDYELLTVLHRS
                     PRLPKCRAQVRRICNALGISLGQLEQDAEKRYRIDIMGLLGIPPAAAVGRVMNIKRAQ
                     AAALWRSIQSKLAGPDLDWFVSIKPII"
BASE COUNT         1476 a         1670 c         1888 g         1355 t
ORIGIN      
        1 gctggatgct tccgcttcag cgccccccgc tgcgattggg gccgccgccg cactgctcag
       61 gccccaggtg ataaagggcg ccaatccttc gctcatgtac attgtcacta atttgccgct
      121 cggtgagatt gaatttccga atatgaatct gaagtgggcg tcaacccggc cggatatcat
      181 tgatgagcag gggcgggtta gccgtccggt tggatcagaa cctgtggcgg tggaactgag
      241 cctgcacctg aagtccgacg gcgctgagac cagtcgggtt tataaggccg cagtgcagcc
      301 gcagaaaaaa tctcttcccc ttgtacaggt taaggctcct gatttcccaa acaacatgta
      361 tcggatcccc tgtgtgggcg ggatatccgg acatgaagag attctgcctg aaagccccgg
      421 tgagattaga atccgcggca acagtgctat tcgctatccg aaaaagagct attctattgc
      481 tctcgaccgg cctcatggcg tatttggtga tacggactcc cggcaccttt acctggtggg
      541 gtgcgggaag gatccctcct tgatcaggaa caagctggcc tatgagctct ttcgcgattt
      601 cggttcagaa gcggctccgc gcatcattcc gcgtgtcgaa atggtcgaac tgattctgaa
      661 cggggagtat ctcggaatct atcagctgac ccagagggtg gaccggcata tgctggggtg
      721 gaaggctttc aactcctgcg aacagcacca caatgtgctc tacaaggcgg acggcccggg
      781 cgcgggattt ttagaggtgc accaggatca gtacgagcag aaagagccgg acgaagtcca
      841 gtgtccgtgc tgggagccgt acagcgagat cattcgattc attgcggaat cctctccgga
      901 agagttccgg gctcatgtcg ctgaacacat ggacatcgcg aatatcgtgg attttcatct
      961 gtttcttctg ctgacgaaca atcgtgacgg caacaaccat aatttatatc tggcccgtga
     1021 aggaacgacg ggaagtcgtt ttttcattat tccatgggac tatgataccg ctttcatgga
     1081 tgacggattc ctttcaaacg gactctttga acgtctcgaa gccgatcttc ccggctatcc
     1141 cggcatgctc aaagagcgct gggtgcagct gcggcaaggc atctgcgaca agcaggccat
     1201 attcgaccga atcgacgcca tgcatgccga aaatgcgccg gcagtggaac gggagaaaat
     1261 cggggaagtg ccgggcgcga agcggcgtga tgctgccgtg cgcgagctga aagcctggat
     1321 atccgcccgt ctgctcgaag tggaccggca tctcgcctcc atgccgtaag tttcgcgatc
     1381 atactcggat attcttgtgg actatccttc tggttgtccg gttattccgg agcagaatca
     1441 tcatccgaaa tggagggggc agcggatcgg tcccgctgtc gcggcatcca tcattccggc
     1501 tttttatctt cgggcttgtc gccggcgatt tccttcatgg cctcgtcctt gccctttttg
     1561 aattcgctga ggctcttgcc cagcgaacgg gccaggtcgg ggaggcgctt cgctccgaaa
     1621 agcagaagga cgatgaacag aatcaggatg acttcccccg cacccggacg accgctgaaa
     1681 aatgccaatg tgttcatgtt aacctcacgg cttctgtttc actttcagaa tgatgatgca
     1741 gacctcgtac aataaaatca atggaatagc catcaaaagc tgcgtgatca catcgggcgg
     1801 cgtcagcaac atggccagaa ccagcaggcc cacgatgacg tgcctgcgct tttcagccag
     1861 catttttgca ttcacgatcc ccaggctgcc cagcacaaat acgatgaccg ggagctgaaa
     1921 agccaggccg aatccgatga ggagcccgag acagaaccgt acgtagctgc tgacggtcca
     1981 aatggccgtg atccccatcc agtcatgcat gcgcatcatc atctgcagag ctgccggaag
     2041 tgtggtccac caggcaatca ctacgccgga aacgaaaaga gcaagcgaaa agcagaaggc
     2101 cttttttatg gctgaccgct cggcatcctt caggcccgga aagacaaacc gcgtgatgaa
     2161 gaacagcatc agcggggagg tgatcagcag gctgccccat ccggcaatgc gcagactgac
     2221 ggaaaaggct gcgccgactt ctatggactg aagaaactgt ccgggattga tcacggtccg
     2281 agccaggggt tctctcagca gattcagaat cgctggagcg agcggaaagg tgatgaaaag
     2341 cccgccggta aagaccccca ggcaccacaa gatggtccgg cgcagttctt ccagatgctg
     2401 gaggaagggc attgtgcgcc cgggttctgg aacgcctgaa ttcatggact cagcgccttt
     2461 gtttccgcca actgcccaca ggggtccttg gcggcgggga tgatattcag aacctgaatc
     2521 tgtgtggtga agctgatggc ttcatcttcc acaaggttcg tctccgtgct gaggatttca
     2581 agctccgcat aacccagccc tgagttcaca tagatttccg tgcagatttt tccgtccggt
     2641 tcgccgctgt ttgcggcggc tttcattatc actgcctgtg gtcccttgaa ggcggccata
     2701 cgcgcgacag gcgcgcggct gccgattttt gtttcttcgc ttgtgcccgg ttcatagacc
     2761 acagaacttc cgcatgtctg aatttgttcg ggatcaggcg ggctgaattt cagtgttttc
     2821 catccaggag aaccttcctg ctccaggcct gtttcgaagg cgattcgttc ggggagggga
     2881 acctgaacga ggttccagat ggtgacagga acagaagatt tttctgtccg caccaggctt
     2941 tgtcgaattg tgatgaagtc gttggagggc atcagccgat aaagctggcg ggcctcgacg
     3001 tgaagcggtt ctccgaaatg tcttgttatg acacacgctg cggctccgtc agcctcggtc
     3061 cacgcgaaac cggtccacgg ttttcgatca atcacagatg aggggggcca gtcgtttgtc
     3121 tgaaaggagg gccaggccga ctgtgccgcc ggccacatcc actgtccgcc gaaatttttc
     3181 cagtctttcc cgtttttccg aggataggat tttccggcca gcgacttgtc cagacgcaga
     3241 aggttttcgc cgccggccgg cccgaaatgg atcatacggc cggcttccgg aacaatcacc
     3301 gcatcaacca gcccgttggc gaggcgaaac gagtttgtcc aacccatgta ttcgcctcct
     3361 gctctcggcg cgatgggaac ctgaggcggc tgagaaaagg ctgtgacagg agtcagcacc
     3421 gcgagcagca ggattttctt ggccttattc atgaacaggg gctagactat aagcatgaaa
     3481 atcaaaacac aatggataaa tatgctggcg ctgcttatcg cgggcggatt ttatgcatct
     3541 gctgcgcacg gggagtacag ctttatgaga actgagctgt ttttccgcct gggcggcgag
     3601 ggggcaaatg tatccgggca ggagtgggac gattttatgg cccagtatgt gactccgcgg
     3661 tttgagcagc acgtgcagat tgcagaggtt accgaacatt tcctgggaga taaaggccgg
     3721 gaactgatcc ctgtaaaggt cctgacctgc cttcacgaca gcacacccgc cgccgatgag
     3781 tccattgatt atatccgcgc ctcctatgtc cggctgttca ggaagaaaca ggttgttcgt
     3841 atcagcgttc cggtggcggc atctttccgg caggacatga aggaaccgga ctggcgtgaa
     3901 ctcggcacac tgcagaatgc cgaaatcagg gaaagcagcg gcatggcata cagcacccgg
     3961 ggtcattctg ttctttggac tcataatgat tcgggggaca ccagccgttt ctttgcagtg
     4021 gggctggcgg gcgaagacct tggcacattc atgctggagg gggcgaaggc tgtggactgg
     4081 gaggatatag cctctttcac gtttgacggg aaaagctggc tcttgctggc ggatgtggga
     4141 gacaacgagg agcgtcgccc ggattgtcag ctgtatattg tggaggagcc gcaggcggga
     4201 accagcgcga ccgtttctgt tgagcggatg atcagcttcg tgtacgaaga cggcccgcac
     4261 aactgcgagg ccgttgcggt ggatccggag gagggccgta ttctgctgat cgtgaaaacg
     4321 cgtgacccgg caagcggcgt ttacagtctt ccgctgaatc cagaagatga aaagacaacc
     4381 gcccggagaa tcgcggatat tcgcatgacg ggtgttgtgg ccatggacat cagccctgac
     4441 ggccgcgatg ccgttgtgct gacctatgga gatgccttcc gctggcgccg cgcagaagat
     4501 gaagggtggg cgtccgcctt tggccgcgaa ccggaaatgc tcccgatgcc ctaccgcaag
     4561 cagggcgagg ccgtatgttt cacgccggac gggaaaaagc ttctgctcag cagtgaaaag
     4621 aagcctcgtc ctctgctgga agtgacgctg ccctgaagaa gaaaaagggg aaaaagctgg
     4681 tggcggcgga gggagtcgaa cccccgagac tatccctata tgcaaatatg cgattttcct
     4741 aatgttttgg acatattaaa acgctattga cacccccaga cgttagcagt tacgttagca
     4801 ggtgcaaaac gggaaacagc aaaccggaac cggatgcact gaaaaaggga ggcggcaatg
     4861 gcgtcaatac acaggcggaa gggcacgaaa aaatggcacg cggcttttta tgatgcggaa
     4921 ggccggcgct gtatgcgatc cacaggcacc agcaaacgcg gtgatgcaat gtccattgcg
     4981 cttgactggg aagaggccgc ccggcggggc agggaagggc gcttgacggt ggatcaagcc
     5041 cgcaaagtca ttgcggacat atacgccaga gcaaaccggg acaagctgcc ggcgggcacc
     5101 gtccgggaat atgtaaagca ctggatgcag atgaaagagg cggggcttgc agattcatcc
     5161 ttcacagaat acaagcgcct gactgccgat ctgattgact ttctgggcaa gcgggcggat
     5221 ggaccgcttg acgctatcac aagcaaggac attgtgaagt atcgcgcccg gctgctggcg
     5281 cgtgtatcgc cggcaacggt gaataaaagc ctgaaagccc ttcggggaat atggacacag
     5341 gcccgcaagg atgaactgat aacgcgcaac gtttttgatt ctgtttccta tgtgaaggcc
     5401 gaacagaaaa agacgcgccg caaaaacttc acaggggaac aggtgaaggc gctgcttccc
     5461 tattgcaagg attcatggcg ggggcttgtg ctggcggggc tttacaccgg acagcgtttg
     5521 tccgatctgc tggcgcttac gtgggaagag atagaccttg ccggggcgaa aatccaattc
     5581 acaacgcaaa agacgggggc ggaacttgaa atccctattc atcccgcgct tatgaaatgg
     5641 ctaatggaca atgccggcga tgatcccgcg gcgcttgtgt tcccggactt cgccggaatt
     5701 tcacagaaca ccgtttcgcg ccagtttatg aaaaagctgc acaatgccgg actgataaag
     5761 gcggaacctt cacacaagag cgcgaacaag ggacgggaca gcaagcggac cctgaatgaa
     5821 ttgagctttc acagtctgcg ccatgcttac gtgacaatga tgaaggaagc cggcgcaacg
     5881 gatgggatta caaagtctat tgtagggcac cagtccgatg cggtgaacag gctttatact
     5941 catatcggaa ttgacgctat gcgcgaagca gtgaacaggc tgccggatgt aactgcgggc
     6001 taaattatcg gctttattga cacaaaccaa tccaagtccg ggccggcaag tttgctttgt
     6061 attgagcgcc agagggccgc tgcttgtgcg cgttttatgt tcataacgcg cccgacagca
     6121 gccgcgggcg ggatgccaag caaccccatt atgtcaatgc ggtatctttt ttctgcatct
     6181 tgttcaagtt gcccgagact aatgccgagt gcattgcaga tgcgccggac ctgtgcccga
     6241 catttaggca accgcgggga ccggtgcaaa acagttagca gttcatagtc agagataata
     6301 gctttctctt caggggaccg ggcaaagaca agggccggaa cgctgttcca gaaaagaacc
     6361 attgcgggca gcggactttt cccgcgggc
//
LOCUS       DAOQWR010000139         6280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_12774_length_6280_cov_2.960161, whole genome shotgun sequence.
ACCESSION   DAOQWR010000139 DAOQWR010000000
VERSION     DAOQWR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12774_length_6280_cov_2.960161"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKK36_09935"
     CDS             <1..1006
                     /locus_tag="PKK36_09935"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNR94909.1"
                     /translation="LTALINEKYMPTLYDQFFKKTNYLVNRLKAKAKTFNGRKIVVPL
                     EYADGSNAQATTKYQHINLAPIDPITAAEYEPKMITDSLIISLEEELVMNSDMAVKNI
                     LDAKMKNVLKGTQKYFLTRFWARSTATADWNSLYTAVLNSGTFGGIDSATYTWWQSHV
                     LDAATLGGGDPSLEADLMDPTKPTYLKKLLQRGIALCKTQTGEDPDIINVPQYIWDLL
                     ELILDPQKTGSKMSEKAGSMGFKALDFRGIDIVADNAMVVAQTGATDGYMEFLNLDYL
                     WMFFNSGAKFKAQPFVRPGNANYKSCLINAYGNLCVSNRGAHCRIINVRSPKEYAGLS
                     "
     gene            1102..1341
                     /locus_tag="PKK36_09940"
     CDS             1102..1341
                     /locus_tag="PKK36_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94910.1"
                     /translation="MAIPVQTAADKAGMAIMAEATVDGVYMLQFGAIKIGMGTGSPNG
                     EVTAPEGSLFVEKGDHELWMNTDGAKTWEIVGTQS"
     gene            1428..2216
                     /locus_tag="PKK36_09945"
     CDS             1428..2216
                     /locus_tag="PKK36_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94911.1"
                     /translation="MKFKILGALLIVALLFGLAGLASGPVESQDRWSQQVASTLVNSK
                     GILFFDAKNYNTFFDDFNFFNASLWDTTSVVDSRGSAFAVVDSATGGLLKISIGAFKD
                     DAANVQSINAPFKLGFSPGRPDSSTVETVFEARFMVSEKTQSVIVAGLCAEDEDLAGA
                     VQDGIYFWKPDQTDSLYVSAKKAGTGVFKYAGVAVANRTMVRLKIEWNGSRARFYVND
                     VYKTYISTTANIPVVNLKPSFEVACGDSVAHFAYLDYILARQKR"
     gene            2325..2627
                     /locus_tag="PKK36_09950"
     CDS             2325..2627
                     /locus_tag="PKK36_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94912.1"
                     /translation="MNKKKLEEAPRDSPGAVPSTPSTSSGAGEVAALPKSRFVQIGGE
                     IEEDGTVSIISAWDVPNCGVVMRTKNFTKGGQFISVSEVFIPMAGVREGKITRSLY"
     gene            2673..3158
                     /locus_tag="PKK36_09955"
     CDS             2673..3158
                     /locus_tag="PKK36_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94913.1"
                     /translation="MLTGGLLDLLDRIRGKTAGAATFDRKKHSMEAVGDDVDTLVAVH
                     AAEAVLKIASDSDTDNITYANVVNISDKGRLKCIYANLKSDNHDPISGSIEITIDGGT
                     AQTATFLANQISTDSSANNIYHGTVSISLDVPFSTSLLVRHKKANTYGKIVTTVVYTT
                     D"
     gene            3170..5476
                     /locus_tag="PKK36_09960"
     CDS             3170..5476
                     /locus_tag="PKK36_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR94914.1"
                     /translation="MPDIIIVVTDEKCAGLKAAAKRDGKTAEKLAQEQMDYYTDVVIK
                     DFVAQPVELTAGKNLFAFSQTLTTPGFYHYEARVEAPGDPLPQNNRAFAFATVRGEPR
                     LLVISNDPELDGPLVAALQSAQLEIRVTGPEQFPDSLAALQSYDAIFLSNVGAGDLGR
                     DRLRLLESAVRDFGVGLVCVGGDEAFTAGGYRGTPLEDALPVSMELDSKKVLPNGALA
                     LVIDRSGSMQGEKMEMAKAAAIGALAALGDQDYVAVIAFDSTFHEIAPLQRASHRRAI
                     MRDVAGINAQGGTVMHPPMARAYEMLKGAKASLKHCVVLTDGQSQPGDFEGLVRAMVA
                     DRITLSTVGVGSDIDEALLQSLAALGGGRFYPVQFAGELPQVFIKETAIILKSAIHEE
                     PFQPQLKAVTELVRGIGGAELPSLQGYVVTTPKPRAETPLWTDKGDPLLAHWQYGLGR
                     VVAFTSDARSKWAKHWLGWAKYRQFWSQAAQWSLRRLANADFTAEVTLDQGEGLLTVE
                     AVDAHGDYRNFLELQATVVSPAGRRETVTLQQTGPGHYEARFPTREVGAYLLQLMDME
                     NGQVRGAQVVGASVNYSPEFNASEPNWALLRRIAESSGGRVLNPALAKDNPFLHNRRK
                     TYQARELWETLLKLAIVLFVGDVGVRRVQWGREEWQRGARVLRRWVFFWKSTPRPVET
                     QESLATLLARRDQVRAAQVFADPERRSELFQPAQPPSPETVAATVHTPQSAGPPPTEP
                     PADASAAPPASTASRLLAAKRRAHQKRG"
     gene            5658..5921
                     /locus_tag="PKK36_09965"
     CDS             5658..5921
                     /locus_tag="PKK36_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNR94915.1"
                     /translation="MGVFYSGRVQGVGFRYTVRTVANGYEVTGTVRNLPDGRVELVAE
                     GTRAELDAFRQAIRDAGLAHFIRDEAVMWSAAAGDLRGFEIVR"
     gene            5928..>6280
                     /locus_tag="PKK36_09970"
     CDS             5928..>6280
                     /locus_tag="PKK36_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR94916.1"
                     /translation="MKFAIIADIHGNLEAFRVVLNDAQKQRCTHYACIGDVVGYGANP
                     AECLALVRKMGMPCVKGNHDEYCSIDEQTEGFNPAAAEAVQWTRKQLSEDGRQWLRDL
                     KYVRMVKNFTIVHATL"
BASE COUNT         1410 a         1673 c         2058 g         1139 t
ORIGIN      
        1 cctcacggca ttgattaacg aaaagtacat gccgacgctg tacgaccagt ttttcaagaa
       61 gacgaattac ctggtgaacc gtctgaaggc gaaggccaag acgttcaacg ggcgcaagat
      121 cgtggtgccg ctcgaatacg cggacgggag caacgcccag gccacgacga agtatcaaca
      181 catcaacctg gcgcccatcg atccgatcac cgcggcggag tacgagccga agatgatcac
      241 cgattctctc atcatctccc tcgaagagga actggtgatg aactccgata tggcggtgaa
      301 aaacatcctc gacgccaaga tgaagaatgt cctgaagggg acgcagaagt atttcctcac
      361 gcggttctgg gcgcggtcca cggcgacggc ggactggaac agcctctaca cggcagtgct
      421 caacagcggg acgttcggcg ggatcgactc ggccacctac acctggtggc agagccatgt
      481 gctggacgcc gccacgctgg gaggcggcga tccgagcctg gaagcggacc tgatggaccc
      541 caccaaaccc acctacctca aaaaactctt gcagcggggc atcgccctct gcaagacgca
      601 gaccggcgag gaccccgata tcatcaatgt cccgcagtac atctgggacc tcttggaact
      661 catcctggac ccgcagaaga cgggaagcaa gatgagcgaa aaggcgggca gcatgggatt
      721 caaggcgctg gatttccggg gcatcgacat cgtggcggac aacgctatgg tggtggcgca
      781 gaccggggcc accgacggat atatggaatt cctgaacctg gactatctct ggatgttctt
      841 caattccggg gcgaagttca aggcacagcc attcgtaaga ccggggaacg cgaactacaa
      901 gagctgcctg atcaacgcct acggcaacct ttgtgtgagc aaccgcggcg cgcactgccg
      961 tatcatcaat gtgcggagcc cgaaggagta tgcgggactt tcgtaaaatc tgaaatgtga
     1021 aatctgaaat ctgaaacgac cggggcggcg cttcgacatc agcgaccgct ccggacataa
     1081 ccagcaacac ggaggtatga aatggcaata cccgtacaga cggcggcgga taaagccggg
     1141 atggcgatca tggccgaggc gacggtcgat ggcgtgtaca tgctccagtt cggcgcaatc
     1201 aagatcggca tgggcaccgg gtcgcccaac ggtgaagtga ccgcgccgga aggttcgttg
     1261 tttgtcgaga aaggcgatca cgaactctgg atgaacacgg atggggcgaa gacctgggag
     1321 attgtgggaa cgcagagcta agttagcggc gctccgacaa gctcagcgac cggaaggaac
     1381 agaagtgata accaaaacgc atgggagata tggaaaatat gggatgcatg aaattcaaaa
     1441 ttctgggggc gctgctgatt gtggcgctcc tgttcgggct ggccgggctg gcgtccggac
     1501 cggtggagag ccaggaccgc tggagtcagc aggtggcgag caccctggtg aactccaagg
     1561 ggattctgtt tttcgacgcc aagaactaca acacgttttt cgatgatttc aactttttca
     1621 atgcgagtct gtgggacacc acgagcgtgg tggattcccg gggaagcgca ttcgcggtgg
     1681 tggactccgc gacgggtgga ttgctgaaaa tatccattgg ggctttcaag gatgacgcgg
     1741 cgaatgtaca gagcatcaat gcgccgttca agctgggatt ctccccgggc aggccggaca
     1801 gttcgacggt ggaaacggtg ttcgaagcgc ggttcatggt atcggagaaa acccaaagcg
     1861 tcattgtcgc ggggttatgc gccgaagacg aggacctggc gggagcggtg caggatggaa
     1921 tttacttctg gaagcccgat caaaccgatt ccctgtatgt gtcggcgaaa aaagccggaa
     1981 cgggagtatt caagtacgcg ggggtggcgg ttgccaaccg gacgatggtg cgactcaaaa
     2041 tcgagtggaa cgggtcgcgg gcgagattct atgtcaacga tgtgtacaag acgtatatct
     2101 cgaccacggc caatattccg gtagtgaacc tgaaaccatc ttttgaagtg gcatgcgggg
     2161 attcggtggc gcatttcgcc tatctggatt atatcctggc gcggcagaag cgataaggca
     2221 ggcagaagtc agaagccaga agtcagaagg gaaaaaacac ggaagaggcg gaccgggcgc
     2281 ttcgatatca gcgaccggtc cgctggaaac gggaaaggga tgatatgaac aagaagaaac
     2341 tggaggaagc gcccagggat tctcccgggg cggtcccttc gaccccttcg acaagctcag
     2401 gggccggaga ggtggcggcg ctgccgaaga gccggtttgt gcagatcggc ggggaaatcg
     2461 aggaggacgg gacggtgagc attatcagcg cctgggatgt gccgaactgc ggcgtggtga
     2521 tgcggacaaa gaatttcacc aagggcgggc agtttatcag cgtgagtgag gtgtttattc
     2581 cgatggctgg agtgcgcgag ggtaaaatca cgagaagcct gtactgaaat ctgaaatgga
     2641 aaatctgaaa tctgaaataa aagagggatg atatgctgac cggcggacta ttggatttgc
     2701 tggaccggat acgggggaaa acggcgggag cggcgacatt cgaccggaag aaacacagca
     2761 tggaggcggt gggggatgat gtcgatacgc tggtggcagt acatgcggca gaggcggtgc
     2821 tcaaaatcgc atcggattcg gacaccgaca acattacata cgcgaatgtg gtaaacatct
     2881 ctgacaaagg ccgtctgaaa tgcatttacg caaacctgaa atcggacaat cacgatccga
     2941 tttccggcag tatcgagatt acgattgatg gcgggaccgc ccaaacggcg acatttctgg
     3001 ctaatcagat aagcacagac tcttcagcga ataatatcta tcacggaaca gtatcaattt
     3061 ctttagatgt gccattttcc acctctctcc tggttcgaca caagaaggca aacacctatg
     3121 gcaagatagt tacaaccgtg gtttacacca ccgattaaag gatttcatta tgccggatat
     3181 aattatcgtg gtaacggacg agaaatgcgc gggactgaag gcggcggcga agcgggacgg
     3241 gaagacggcg gagaaactgg cgcaggagca gatggattat tacaccgatg tggtgataaa
     3301 ggatttcgtg gcgcaaccgg tggaactgac ggcgggcaag aatctgtttg cgttttcgca
     3361 gaccctcacc acgcccggtt tttatcacta cgaggcgcgg gtggaagcgc cgggcgatcc
     3421 gttgccgcag aataaccgcg ccttcgcgtt tgccaccgtg cgcggcgaac cgcggttgct
     3481 ggtgatctcc aatgacccgg agctggacgg cccgctggtg gcggccttgc aatccgcgca
     3541 actggagatc cgcgtcaccg gcccggaaca atttcccgat tcactggcgg cattgcagag
     3601 ctacgatgcc atcttcctca gcaacgtggg cgcgggcgat ctgggccgcg accggttgcg
     3661 gttgctggag agcgcggtgc gggattttgg tgtggggctg gtgtgcgtgg gcggtgacga
     3721 ggcgtttacg gcgggcggtt accgcggcac gcccttggaa gacgcgctgc ccgtgagcat
     3781 ggaattggac agcaagaaag tgctgccgaa cggcgcgctg gcgctggtga ttgaccgttc
     3841 gggcagcatg cagggagaaa agatggagat ggccaaggcc gcggccatcg gggccctggc
     3901 cgcgttgggc gatcaggatt atgtggcggt gatcgcgttt gacagcacat tccacgaaat
     3961 tgcgccgctg caacgggcct ctcaccgccg cgccatcatg cgcgatgtgg ccggtattaa
     4021 cgcccaaggc ggtacggtca tgcatccgcc gatggcccgc gcctacgaaa tgctcaaggg
     4081 cgccaaagca tcgctcaaac attgcgtcgt gctgaccgat ggccagtccc agccgggaga
     4141 tttcgagggc cttgtccgcg ccatggtggc cgaccggatc accctttcca ccgtgggcgt
     4201 ggggtcggac attgacgaag ccctgctgca aagtttggcg gccctgggcg ggggccgttt
     4261 ttatccggtt caattcgccg gcgaactgcc gcaggtgttc atcaaagaga ccgccatcat
     4321 tttgaaatcc gccatccacg aagaaccttt tcaaccgcag ttaaaagccg tcaccgaact
     4381 ggtgcgcggc atcggtggcg ccgagctgcc ctcgttgcag ggctatgtgg tcaccacgcc
     4441 caagccgcgc gcggaaacgc cgctttggac cgataaaggc gatccgttgc tggcccattg
     4501 gcaatatggt ctgggccgcg tggtggcgtt cacgtcggac gcgcggtcca aatgggcgaa
     4561 acactggttg ggctgggcca agtaccggca attttggtcg caagccgcgc aatggagttt
     4621 gcggcggctg gcgaacgcgg atttcaccgc cgaggtcacg ctggatcagg gcgaagggtt
     4681 gctgacggtc gaggcggtgg atgcccacgg cgattaccgc aacttcctcg agcttcaagc
     4741 caccgtggtc agcccggccg gacggcgcga gaccgtgaca ctgcaacaaa ccgggccggg
     4801 ccattacgag gcgcgctttc cgacacgcga ggtgggcgcg tatttgctgc agctgatgga
     4861 catggaaaac ggtcaagtgc gcggcgcgca ggtcgtgggc gcgagcgtga attattcgcc
     4921 cgagttcaat gcgtccgagc cgaattgggc cctgctccgg cggatcgccg agagcagtgg
     4981 cgggcgggtg ttgaatccgg cattggcgaa ggataatccc ttcctacaca accggcgaaa
     5041 aacctatcaa gcgcgcgagc tttgggaaac gctgctcaaa ctggccatcg tgctgttcgt
     5101 tggcgacgtg ggcgtgcgcc gggttcagtg gggccgcgaa gaatggcagc gcggggcgcg
     5161 tgtcctgcgc cgatgggtgt ttttctggaa aagcacgccg cgtccggtgg aaacccagga
     5221 atcgctggcg accttgctgg cgcgccggga tcaggtccgc gcggcacaag ttttcgccga
     5281 ccccgaacgc cgatccgaac tgtttcaacc cgcgcaaccg ccgtccccag agaccgtggc
     5341 cgcaacggtt cacacgccgc aatccgccgg cccaccgcct accgaaccgc ccgccgacgc
     5401 atccgcggcg ccgccggcaa gcacggccag ccggttgttg gccgccaaac gccgcgcgca
     5461 tcagaaacgc gggtagcggg cggggccggg ttggcccgac aggttgtcgg gtaaaatccc
     5521 cggagaaaaa cattgcggca accggacggt ttatggcttt atcctcgccg ttaaaagcgg
     5581 gacggctggc gccggcccgc gactgagtca ctgcaaaaca actcgatgga atgactgccg
     5641 cggcgaaccg ttgccggatg ggcgtgtttt actctggccg cgtccagggt gtgggctttc
     5701 gctacaccgt gcgaacggtg gccaatggtt acgaggtgac gggcacggtg cgcaacctgc
     5761 cggatgggcg ggtggaactg gtcgccgagg ggacgcgggc cgagttggac gcgttccgcc
     5821 aggcgattcg cgacgcgggt ctggcccatt ttattcggga cgaagcggtc atgtggagcg
     5881 cggccgcggg ggatttgcgg ggctttgaga ttgtgaggta ggacggaatg aagtttgcca
     5941 tcatagcgga cattcacggc aacctggagg ctttccgggt ggtgctgaac gatgcccaga
     6001 aacaacgctg cacacactat gcctgcatcg gggatgttgt tgggtacggg gcgaatccgg
     6061 cggaatgcct cgcgctggtc cgcaaaatgg gcatgccctg cgtgaagggc aaccacgacg
     6121 aatactgctc gattgacgaa caaaccgagg gattcaaccc ggccgccgcc gaagccgtgc
     6181 agtggacccg caaacaatta agtgaagacg gccggcagtg gttgcgcgat ctgaaatatg
     6241 tgcgcatggt gaaaaacttt acgatcgtcc atgccaccct
//
LOCUS       DAOQWR010000140         6046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13368_length_6046_cov_5.251711, whole genome shotgun sequence.
ACCESSION   DAOQWR010000140 DAOQWR010000000
VERSION     DAOQWR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13368_length_6046_cov_5.251711"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="PKK36_09975"
     CDS             complement(<1..1533)
                     /locus_tag="PKK36_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94917.1"
                     /translation="MAARPFESRLQDLVYNVDVGVGREIIRFGLYVLFFLFIILFYTV
                     VQFKGLKEEEAMDYAQLGRNLAQQKAFVTQCVRPVTAGFFLKRPEVQNPLIMRHPDIL
                     HPPLYPVLLGTAFKLSGVPFAVETNARTFPAEQRVIIPLSHLFSILTGILVYMIGRRL
                     FDRRVAVTGMIIFYLSDWVWRESCSGLPLSMTAFLAALATLLTVQAARMRAQPAVRAR
                     SWLVPLVLAAVTCAAAALTRYGAVVLVPMLGLYVYLTFGRRAWAWLLVFLLIFSACVS
                     PWLVRNYRVSGKILGLAPQTALIGSSAFPDDSFFRTLNPDLSYDRVQPSLTDQWLKRM
                     GEAFSENLKLQRAGLLGCFFLVTFFYGFVRPQVQRLRWCVLLGLLLLVMLAGLYGEST
                     SRLSLIFWPMILLYGLSFFFILLDRFEFAMPILNKALTAFLVLLCSLPMLFALLPPRP
                     GIPYPPYFQPYIVHVSAILKPNELMCTDMPWATAWYGQRTSLQLPQTLDEFYQINDLY
                     QKI"
     gene            complement(1604..2968)
                     /locus_tag="PKK36_09980"
     CDS             complement(1604..2968)
                     /locus_tag="PKK36_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNR94918.1"
                     /translation="MNLPTVLIVDDEKNTREGLSRALRKNYRVLLAEHGHQALEIMDR
                     EPVDVLLSDIRMPGMDGLTLVQRALTREPQPVCILLTAYGSIEMAVEAMKRGAYDFLT
                     KPVNLDKVEILLKRALDSRSIEAENRRLHEQLDSKFGMENLIGESAPMQQVFDTVRQV
                     AAARATVLIEGESGTGKEMAAHAIHRLSPRAKGPMIALHCAALSDSLLESELFGHEKG
                     AFTGATERRRGRFEAADGGTLFLDEIGEISPAVQVKILRVLEERCFERVGGQETIDVD
                     IRLIAATNRDLKKMVEEGKFREDLYYRLHVVSIRMPPLREHPEDIPLLCRHFLDQFNR
                     ENSTALAGFTPDALEAMTQYDWPGNVRELRNAVERMVVMARGDKLTLRDIPAPIRDAA
                     APRAGRGAPAALSLEHAERQMIVEALRATGDNRTLAAERLGISRRTLHRKLNEYGLRG
                     DGKQ"
     gene            complement(2965..4152)
                     /locus_tag="PKK36_09985"
     CDS             complement(2965..4152)
                     /locus_tag="PKK36_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94919.1"
                     /translation="MKAKLLDKLIDHLDVLDAESLQGHFLTLARERRLMETIFHAVQE
                     GIVALDGEGNITYANRASEKLMGFSFDEAGGTPIRRYLREVDWDHLLDFNEEDTSRWM
                     VREVCTSYPEPRVLQFYVVPLSMVNRKEGGAVLIFRDITRDRRDEENMRESERLNAIT
                     LLAAGVAHEIGNPLNSLNIHLQLLSRELAALPDEQRKELRELVDVATSETRRLDQIIN
                     QFLGALRPVKMSLERQQLDLILKDTLRFMKKEIENRSVIIEVDVPSSLPPVRADRDQV
                     RQAFFNVIKNAIQAMPGGGILKVTLSATDRAVQAAFTDTGVGIEAGDMGRIFEPYYTT
                     KAEGSGLGMMIIHRIMRDHGGEIEVHSEPGRGTAITLIFPREDARFRLLESHTETGKP
                     EDR"
     gene            4538..4795
                     /locus_tag="PKK36_09990"
     CDS             4538..4795
                     /locus_tag="PKK36_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94920.1"
                     /translation="MFTTDDSLNYGMAQGCPVHGDDNMVECSVCGIEYCAACFQSDGL
                     CKDCAAQAEDELSGKEPDFDDVESLDAILSKEELPDVPDER"
     gene            4869..>6046
                     /locus_tag="PKK36_09995"
     CDS             4869..>6046
                     /locus_tag="PKK36_09995"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNR94921.1"
                     /translation="MSDTLTPMMQQYRRIRSELPPDTLLFFRLGDFYEMFFDDAKTAA
                     GILDIALTRRHHTPMCGIPYHAADSYLAKIIRAGKKVAICEQMEDPAQAKGIVRREVT
                     RVITPGTVLQDAVLQSDRNNWLAGLCRTGELFGLALLDLSTGSFWIEESSSPDALREN
                     MLRYSPSECLVPEEQAAQPEVRAMLERQAQAVLTPYEDWTFDAVTASDFLKRHFQVHS
                     LEGFGCETCKAGISAAGAVLHYVTSELHVRADHIRQVRRRNPDDFMLLDESTAANLDL
                     VECRGRRVAGAANTLLGVLDATKTAMGGRLLREWVLRPLNNLDRIQCRHEAVQTLHDN
                     WPLLQDLRETLALVRDMERIIARLSAGGGNARDIRALAASLSALPHIRSLAAGQPVPA
                     L"
BASE COUNT         1210 a         1874 c         1773 g         1189 t
ORIGIN      
        1 gattttctgg tacaggtcgt tgatctggta aaactcatca agggtttgag gaagctgcag
       61 cgatgtgcgc tggccgtacc atgccgtggc ccagggcatg tcggtgcaca tcagctcatt
      121 aggcttcagg atggcgctga cgtgaacaat atagggctgg aaatagggtg gatagggaat
      181 gcccggccgc ggcgggagca gggcaaaaag catgggaagc gagcagagca gcacaagaaa
      241 cgcggtgagg gccttgttca ggatgggcat ggcaaattca aaacggtcca gcagaatgaa
      301 gaaaaaggac aggccgtaaa gaaggatcat aggccagaag atcaggctga gccgcgaagt
      361 gctctcgcca tacagtccgg ccagcatcac cagcagcaga aggcccagca gcacgcacca
      421 gcggagccgc tgcacctgcg gccggacaaa accgtagaag aaggtgacca ggaaaaagca
      481 tccgaggagg ccggcccgct gcagcttcag gttttcactg aaggcctcgc ccatgcgctt
      541 gagccactgg tccgtcaggg agggttgaac gcggtcatag gagagatccg gattgagtgt
      601 gcggaagaag ctgtcgtccg ggaaagcgga actcccgatc agggccgtct gcggcgcaag
      661 accgaggatt tttccgctga ccctgtagtt gcggaccagc cacggggaca cgcatgcgga
      721 gaagatcagc aggaacacca gcagccaggc ccaggcccgg cggccgaagg tcagataaac
      781 ataaaggcca agcatcggca ccagcacgac agccccgtag cgggtcagcg cggcggcggc
      841 gcatgtcacg gcggccagca ccagcggaac cagccagctg cgcgcgcgca cggcgggctg
      901 cgcgcgcatc cgggccgcct gcaccgtgag cagggtcgcc agagcggcca ggaatgcggt
      961 catggaaagc ggaaggcccg agcagctctc gcgccagacc cagtcgctca aataaaagat
     1021 gatcatcccg gtcacagcga cccgccggtc aaacaggcgg cggccgatca tatacaccag
     1081 aatgccggtc aggatgctga acaaatggct cagcggaata atgacccgct gttcggccgg
     1141 gaaagtccgc gcgttggtct ccacggcaaa aggcacaccg ctgagcttga acgccgtgcc
     1201 gagcaggacc ggataaagcg ggggatgaag aatgtcggga tgccgcatga tcaggggatt
     1261 ctgcacttca ggtctcttga ggaagaagcc ggccgtgacc ggccggacgc actgcgtcac
     1321 aaacgccttc tgctgcgcca ggttgcgccc gagctgcgca tagtccatgg cctcctcctc
     1381 cttgaggccc ttgaactgca ccacggtgta gaaaaggatg atgaaaagga agaacaggac
     1441 gtaaagtccg aaccggatga tctcgcgtcc gactcccaca tccacattgt aaaccaggtc
     1501 ctgaagcctg gactcaaacg gccgtgccgc cataactgtt acctgtcctt tccgtttgca
     1561 gaaaacatat cagcttgcca aaggctcgta cagaagcaac gcgtcattgt ttgccatccc
     1621 cccgcagtcc gtactcgttc agtttccggt ggagcgtcct tcggctgata cccaaccgtt
     1681 cggccgccag cgtccggttg tctccggttg ccctgagcgc ctccacgatc atctgccgtt
     1741 cggcatgctc cagcgaaagc gcggcgggag ccccccggcc ggcccgcggc gccgctgcat
     1801 cgcgaatcgg cgccgggatg tcgcgcaggg tcagcttgtc gccgcgcgcc atcaccacca
     1861 tccgctcgac cgcgttgcgg agttcgcgga cgttgcccgg ccagtcgtac tgcgtcatgg
     1921 cctccagcgc gtccggcgta aatcccgcga gggccgtgct gttctcgcgg ttgaactggt
     1981 ccagaaaatg ccggcacagc agcgggatgt cctccggatg ctcccgcagg ggcggcatgc
     2041 ggatggacac cacgtgaagc cggtaataga gatcctcgcg gaattttccc tcctcgacca
     2101 tcttcttcaa atcccggttt gtcgcggcaa tcaggcggat gtccacatca atggtctcct
     2161 gccctccgac gcgctcgaag caccgctcct ccagcacacg caggattttc acttgcaccg
     2221 ccgggctgat ttcgccgatc tcatcaagga aaagcgtgcc gccgtcggcg gcctcgaaac
     2281 ggccgcggcg ccgttccgtg gcccccgtga atgcgccctt ttcatgaccg aaaagctcgc
     2341 tctccagcag cgaatcggaa agagcggcgc agtgcagcgc aatcatcggg cccttcgcgc
     2401 gcggactcaa cctgtgaatg gcatgggccg ccatttcctt gccggtaccg ctttcgcctt
     2461 cgatcagaac cgtcgcgcgc gcggcggcca cctggcggac cgtgtcgaac acctgctgca
     2521 tcggggcgga ttcgccgatc agattctcca tcccgaactt ggagtcgagc tgctcgtgaa
     2581 gccggcggtt ttcggcctcg atgctgcgcg agtcaagcgc ccgcttcagc aggatttcca
     2641 ccttgtcaag attcaccggt ttcgtcagaa aatcgtacgc gccccgcttc atggcctcca
     2701 ctgccatttc aattgaacca taggcggtca gcaggatgca taccggctgc ggctcgcggg
     2761 tcagcgcgcg ctgcacaagc gtcaggccgt ccatgccggg catgcggata tcgctcagca
     2821 gcacatccac cggctcgcgg tccatgattt caagcgcctg atgtccatgc tcggccagca
     2881 gcacccggta attcttccgc agcgcccggg acagtccctc gcgggtgttt ttctcatcat
     2941 ccacaatcag cacagtgggc agattcatcg gtcctccggt tttccggttt cggtgtggga
     3001 ttccagcagg cggaagcggg catcctcgcg cgggaagatc agcgtgatgg cggttccgcg
     3061 gcccggttcg ctgtggactt caatctcacc gccgtgatcg cgcatgatgc ggtgaatgat
     3121 catcatgccc agtccgctgc cttcggcctt ggtggtgtag tacggctcga agatccgccc
     3181 catgtccccg gcttcgattc cgactccggt gtcggtgaag gccgcctgca ccgcgcggtc
     3241 cgtggcggac agggtcactt tcaggatgcc gccgccgggc atggcctgaa tcgcattctt
     3301 gatgacattg aaaaaggcct gccgcacctg gtcgcggtcc gcccgcaccg gcgggagact
     3361 ggacggaaca tccacctcga taatgaccga gcggttttcg atttcctttt tcatgaaccg
     3421 caaagtgtct ttcaggatca aatcgagctg ctgccgttcc agactcattt tgacaggccg
     3481 gagcgcgccg aggaactgat tgatgatctg gtcaaggcgg cgggtttcgg atgtggccac
     3541 atccacaagc tcacgcagtt ccttccgctg ttcatcgggc agcgcggcca gttcgcggct
     3601 cagcagctgc agatgaatat tgagcgagtt gagcggattg ccgatctcgt gggccacgcc
     3661 ggccgccagc agggtgatgg cattgaggcg ttcagactcg cgcatgtttt cttcatcccg
     3721 ccggtcacgt gtaatatcac ggaagatgag cacggcccca ccctctttcc tgtttaccat
     3781 ggacaggggc accacataaa actgaaggac ccgcggctcg ggatagctgg tgcagacttc
     3841 gcgcaccatc cagcgggacg tatcctcttc attgaagtcg agcaggtgat cccagtccac
     3901 ctcgcgcagg tagcggcgga tcggtgtgcc tccggcttca tcaaaagaaa agcccatcag
     3961 tttctcggag gcgcggtttg cataggtaat gtttccctct ccgtccaggg caacgatgcc
     4021 ttcctgaacg gcatggaaaa tggtttccat cagcctgcgt tcgcgggcca gcgtaagaaa
     4081 atgcccctgc aggctttccg catccagaac atccagatga tcaatcagtt tatctaaaag
     4141 tttcgccttc atcgttcgaa aaggtgcgcc aatttgtctc tcaagctatg ctcttcctta
     4201 acccctcgca agccattttt tcgcattttt caagaaaaat cgacgatttt gggccgtttt
     4261 tgaccatttt tcgccgtttt ttgctgattt tttggctttt ttgaccattt tttggctttt
     4321 tttaatcttt ttccggtttt ttcacccgtc gccgtcgcat gcaatcaccg ggagcctgaa
     4381 gaatccgcat cccctccccg tccgctgcag agcgcagaat ttcacccccg agttcccgtt
     4441 tttttcatcc tcttttttct ggtcatcagg tttttgttgg tctaggttaa attaataata
     4501 aagctggcag gcatagcagt tctgcacgga ggtgaatatg ttcacgacag atgattcttt
     4561 gaactatgga atggcgcagg gatgtcccgt gcatggcgac gacaacatgg tggaatgcag
     4621 cgtgtgcggc atcgaatact gtgcggcctg tttccagagt gacggtctct gcaaggactg
     4681 cgccgcgcag gccgaagatg agttgtccgg caaggagccg gactttgacg atgtcgaaag
     4741 tcttgatgcc atcctgagca aggaagaact ccccgacgtt cccgacgagc gctgacgcgc
     4801 tcatgctcgc ggcttgcggt aatgattgaa tcgcggcgca ccttgtccta ttctgcatca
     4861 cggaatttat gagcgatacc ctcacaccaa tgatgcagca gtaccggcgg atacgcagcg
     4921 aactgcctcc cgataccctc ctgtttttcc ggctgggcga tttctacgaa atgttttttg
     4981 atgacgcaaa aaccgccgcc ggcatcctcg atatcgcgct caccaggcgc caccacacgc
     5041 ccatgtgcgg catcccctat catgccgccg acagttatct cgcgaaaatc atccgcgcag
     5101 gcaaaaaggt tgccatctgc gagcagatgg aagaccccgc gcaggccaaa ggcatcgtgc
     5161 ggcgcgaagt cacgcgcgtg atcacgcccg gcaccgtcct gcaggatgcc gtgctccagt
     5221 ccgaccgcaa caactggctg gcggggctct gccggaccgg cgagttgttc gggctggcgc
     5281 tgctggatct gtccaccgga agcttctgga tcgaggaaag ctcaagcccc gatgccctcc
     5341 gcgagaacat gcttcgctac agcccctccg aatgcctggt gcccgaagag caggccgcgc
     5401 agcccgaagt ccgcgccatg ctggaacgtc aggcccaggc cgttctgacc ccctatgaag
     5461 actggacctt cgatgccgtc accgcctcgg attttctgaa gcgccacttt caggtgcatt
     5521 cgctggaggg tttcgggtgc gagacctgca aagccggcat atccgccgcg ggtgccgtgc
     5581 tgcactatgt cacgtccgaa ctccatgtcc gggccgacca catccggcag gtgcgccgac
     5641 gcaacccgga cgatttcatg cttctcgacg aatccaccgc agccaacctc gatcttgtcg
     5701 agtgccgcgg gcgcagggtg gccggagcgg cgaacacgct gctgggcgtt ctcgatgcca
     5761 ccaaaaccgc tatgggcgga cgactcctgc gcgaatgggt tctgcggccg ctgaacaatc
     5821 tggaccgcat ccagtgccgg cacgaagccg tgcagaccct gcatgacaac tggccgcttc
     5881 ttcaggacct gcgcgaaacg ctcgcgctcg tgcgagacat ggaacgcatc atcgcgcggc
     5941 tcagcgcggg cggcggaaac gcgcgcgaca tccgcgcgct ggccgcctcg ctctccgccc
     6001 tgccccatat ccgcagtctt gcggccgggc agcccgtgcc cgcgct
//
LOCUS       DAOQWR010000141         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13478_length_6010_cov_4.854408, whole genome shotgun sequence.
ACCESSION   DAOQWR010000141 DAOQWR010000000
VERSION     DAOQWR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13478_length_6010_cov_4.854408"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKK36_10000"
     CDS             complement(<1..1448)
                     /locus_tag="PKK36_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylpolyamine amidohydrolase"
                     /protein_id="HNR94922.1"
                     /translation="MFRIRRIHDIANPANERALAQVLQILRDQFPRLSPKDVDKLPDQ
                     LRNPLKYQLRSILFVADDMKGRVSGFALLMHAPDLNFCYLDYISAAKQLTGRGIGGAL
                     YDRLRTEAALLKVKGLFFECLPDDPLLCREPEVLKQNRARLKFYERYGATPMIGTKYE
                     TPVKPDGDNPPYLVVDPLGQTVRFGRNEMRQIVAAILDRKYKDVCSPDYVRTVTDSIT
                     DDPVRFRPFKYRTEKKVEAVRSAPHPDQLIALVFNNTHAIHHVHDSGYVESPVRVKNM
                     LEALSKTDIFHTIPERHFGEHHITDVHDRAFVDYLQKVCMTLPEDESVYPYVFPIRNK
                     TRPPRDLPMRAGYYCIDTFTPLNKNAYLAARNAVNCALTAAQAVVDGSYLAYALVRPP
                     GHHAERNCFGGFCYFNSTAIAAHYLSRLGKVAILDIDYHHGNGQQDIFYERSDVLTIS
                     IHGHPSFAYPFFSGFMEERGEGKGRGFSINMPL"
     gene            1802..2134
                     /locus_tag="PKK36_10005"
     CDS             1802..2134
                     /locus_tag="PKK36_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNR94923.1"
                     /translation="MNDIPILTTPVVEGAKVMEYKGIVTARNVRAVNVIRDFFTAFRD
                     FFGGRSESYQDVMSGMEKEVFHEIRAAALQMGANAVIGFTLDFDNIGSKNKSLLMAHG
                     KGTAVVIQ"
     gene            2224..2763
                     /locus_tag="PKK36_10010"
     CDS             2224..2763
                     /locus_tag="PKK36_10010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR94924.1"
                     /translation="MSLSSVIMYRLAALAGLLRRAEEMARAETVRRAAKLGKDVFIPG
                     DLIVKKPAKLSIGDRTYVSRGCFFDCKGGLEIGHDCHISTGCVIYTHDHDINEKIPYG
                     KEIAKPVKIGDYVWIGAAARIVPGVTIGNGAVIGFGCLVTGDVGEGEIVGSSAHRVLK
                     TRDPETWQRLLSEKTSSDQ"
     gene            2753..4429
                     /locus_tag="PKK36_10015"
     CDS             2753..4429
                     /locus_tag="PKK36_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNR94925.1"
                     /translation="MINKNVTPSPARPQRWKASGRTPRILLCGVFGPYGVDDPFGRKE
                     NNMELYHNQVTRVQGPASLRFHHRSFGLYFIASNINADVTVLDFPSRRRFIREIQKDY
                     DVVGISFITPNYIKAREMARLVRANAPGSIIVLGGHGAAIEGVEDLIECDHVARGEGI
                     RWMRNFLGQDSSAPIVHPILPCSEYESIFGVPIPGPTPNVLVPGLGCVNACNFCCTTH
                     FFEKAYISFLRTGEDVFRECCRIADARGTNVFSVMDENFLKDCTRARALLAEMEKHQR
                     WFSFYISSSAEAISAFGIDNLIRLGVNMVWIGFESQSADEFAKNAGINAAELVRELRD
                     RGVSVLGSAIICMEHHTPENIGADIDFVVGLGTDMMQFMLYTGMPVTPLYTDHKERGL
                     LKEDMPYEEWHGQNELNWNHPAFPGDEPKRWLDLAFRRDYEANSSSIYRMAETAVRGY
                     KRLAAMESRDPCLEARMQQLALRVRLYSHLLPIIACRAVNEKERERALELDIEQLKLL
                     GPLTVRDRITRLAAKALAARWDLRLRLFGDRIQPSTIVTHYHGGKPDKGL"
     gene            4527..5015
                     /locus_tag="PKK36_10020"
     CDS             4527..5015
                     /locus_tag="PKK36_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNR94926.1"
                     /translation="MSNRNSEQVRDRLVRLAGTISQDLGLGRITGQILVYLYLRPAAC
                     SLDAIEADLGLSKAAVSIAARQLESIGLLKRIRKKGDRKSYYQTAENLGSALQKGLFG
                     LMSRKLEIANGEFQQAIQELEAEKADADAIFLEKRIKRAMVLGKRMDKIISSPLIKLL
                     GR"
     gene            5019..>6010
                     /locus_tag="PKK36_10025"
     CDS             5019..>6010
                     /locus_tag="PKK36_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94927.1"
                     /translation="MRFTEPGKHIRHLVLKTMRVPQSRRYDFMLNEGWNKDYIHNWLQ
                     AYSYWKTMRAKQPRLVRIFSYSRSGTHNFFSRFHYMPCCFVIRENMFEQVDDRLQLRA
                     GIHSLRPLDIMAFSVFGPYGLQDKSAALLTHVFLPSNRFLEYPCLLAPGQMGDDIAIM
                     YVRNYFRMLYSRQKSSEKYNKPRFNITDEVFENWSRSHLEKLSIMVNHIRTRPAQFMM
                     VVHEYFCAHSGEVIDSVCETMGIPPHHRQGWDNPAGFFKRGWACGSIPDVRDGKIWDT
                     RRGCQICGTGDNYNPLPLPSLERTMGDPVADWLTPARLSAARSVFGPELVEFWL"
BASE COUNT         1333 a         1558 c         1763 g         1356 t
ORIGIN      
        1 agtggcatgt tgatgctgaa accccggcct tttccctcgc cgcgctcctc catgaatccg
       61 ctaaagaacg gataggcgaa actcggatgc ccgtggatgg aaatggtcag cacgtcactg
      121 cgctcgtaga atatgtcctg ctggccgttg ccgtgatgat agtcaatgtc caggatggca
      181 accttcccga gccggctcag atagtgggcg gcgatggctg ttgagttgaa atagcagaat
      241 ccgccaaagc agttcctttc agcatgatgt cccgggggac ggaccagggc gtacgcgagg
      301 taggatccat ccacaaccgc ctgggccgcg gtcagcgcgc agttgaccgc gttgcgcgcg
      361 gcaagatagg cgtttttgtt cagcggagtg aatgtgtcaa tgcagtagta gccggcgcgc
      421 atgggaaggt ctctcggcgg ccgggttttg ttgcggatgg gaaacacata gggatagaca
      481 gactcgtcct caggcagcgt catgcagact ttctgaagat aatccacaaa ggcgcggtcg
      541 tgcacgtccg tgatgtgatg ctcgccgaag tggcgttcag ggatggtgtg gaaaatgtct
      601 gttttgctga gcgcttccag catgttcttc acccgcaccg gggactcgac atatccgctg
      661 tcatgcacgt ggtgaatggc gtgggtgttg ttgaacacaa gcgcaatgag ctgatccgga
      721 tgcggagccg agcgaacggc ctcgactttc ttctctgtcc ggtatttgaa cggccggaac
      781 cggaccgggt catctgtgat tgaatccgtc acggtgcgga catagtccgg gctgcacacg
      841 tccttgtatt tgcgatcgag gatggctgca acaatctggc gcatttcgtt ccggccgaaa
      901 cggaccgtct ggccgagggg gtccacgaca aggtaggggg gattgtcgcc gtccggcttc
      961 acgggtgttt cgtatttcgt cccgatcatc ggtgttgcgc cgtaccgttc atagaatttc
     1021 aggcgggcgc ggttctgctt gagcacttcg ggctcgcggc aaaggagcgg atcgtccggc
     1081 aggcattcga agaaaagccc cttcaccttg agaagggcgg cctcggtcct gagacgatca
     1141 taaagcgccc ctccgatgcc ccggcccgtg agctgctttg cggcggaaat ataatcgaga
     1201 tagcagaaat tcaggtccgg cgcgtgcatc agcagggcaa acccgctgac ccggcccttc
     1261 atgtcgtcgg ccacaaaaag aatggatctg agctggtact tcagaggatt tctgagctgg
     1321 tcggggagtt tgtcaacgtc tttgggggac aggcggggaa actgatcgcg cagaatctgc
     1381 aggacctgtg ccaaagccct ttcgttcgcc ggattggcga tatcatggat tctccgtatc
     1441 ctgaacattg gttttcttat atcatggcat cgtgcgcggt tcaagatgcg tcgtccggtg
     1501 caggtttgag gctttcagga aagccctgat attatcgttt gctgggcggg ccttaattgg
     1561 ctaatatatg cgcatgatta agaatcgggg cgggcgtttc cgtcaatcac cgcaccgcag
     1621 cccgaaaaat gacctggccg atgtcgaggc gctcgaaacc ctgaaaaagg cctgaaaacg
     1681 ccttaaaaag tcaaaaaatg gcctaaaaac gcataaaaac ggctccaaaa cgctgaaaaa
     1741 tgcgaaaaat cggctgattt ttaaaaaaac agctgttgaa acaaaaagag gagaagaaca
     1801 tatgaatgac attcctattc tgacgacgcc ggttgttgaa ggcgcgaagg tgatggagta
     1861 caagggcatt gtcacggcgc gcaatgtgag agcggtgaat gtgatccgcg atttcttcac
     1921 ggccttccgc gattttttcg gcgggcgttc cgaatcctat caggatgtga tgtccgggat
     1981 ggaaaaggaa gtgtttcacg aaatccgcgc cgcggcgctt cagatgggtg ccaatgcggt
     2041 gatcggtttc acgctggatt tcgacaatat cgggtccaag aacaaatccc tgctcatggc
     2101 gcatggcaag ggcacggccg ttgtgattca atgagacaaa tggatcaggg ccgcggattg
     2161 agcttgttcg cgccggcgga acaatccatc atctcctgcg atgaatatta atgggaattt
     2221 gctgtgagtc tttcgagtgt cattatgtat cgtctggcgg ccctcgccgg gctgcttcgc
     2281 cgggcggagg aaatggcccg cgcggaaaca gtccgccgtg ccgccaaact cggaaaagat
     2341 gtcttcattc cgggcgacct gatcgtcaag aagcccgcga aactttccat cggcgaccgg
     2401 acctatgttt cccgcggatg cttcttcgac tgcaagggcg ggctggaaat agggcatgac
     2461 tgccacattt ccaccggatg cgttatctac acgcatgacc atgacattaa tgaaaagatc
     2521 ccgtatggga aagaaatcgc gaaaccggtg aaaatcggcg actacgtctg gatcggcgcg
     2581 gcggcacgta ttgtgccggg tgttacgatc ggaaatggcg ctgtgatcgg tttcggatgc
     2641 ctggtgacag gtgatgtcgg ggaaggggag attgtcggct cctccgcgca tcgcgttctc
     2701 aaaacccgcg accccgaaac ttggcagcgc ctgctgtcgg aaaagacttc aagtgatcaa
     2761 taaaaatgtg actcccagcc ccgcgcgtcc acagcgctgg aaagcgtcgg gccgcacccc
     2821 tcgtattctg ctttgcgggg tttttggacc gtacggcgtg gatgatccgt tcggccgcaa
     2881 agaaaacaac atggagctgt atcacaatca ggtgacacgg gttcagggcc ctgcttcgct
     2941 tcgttttcat caccgctcct tcggcctgta tttcattgcc tccaacatca atgccgatgt
     3001 gaccgtgctc gatttcccct cccgccgccg atttatccgg gagatccaaa aggattacga
     3061 tgtggtgggc atctctttta tcaccccgaa ctacatcaag gcccgcgaga tggcacgtct
     3121 ggtccgggcg aatgctcctg gatcaatcat cgttctgggc gggcacggtg ccgccatcga
     3181 aggcgtcgag gatctgattg aatgcgacca cgtggcccgg ggcgagggga tccgctggat
     3241 gcggaacttt ctgggccagg attcgtccgc gccgattgtt catcccattt tgccatgcag
     3301 cgaatatgag agcatctttg gggtgcccat tccgggtcct acgccgaatg tgctggtgcc
     3361 gggcctgggc tgcgtcaacg cctgtaattt ctgctgcacg acccattttt ttgaaaaggc
     3421 ctacatttcg ttcctgcgca cgggcgagga tgtctttcgg gaatgctgcc gcatagccga
     3481 tgcgcgcggc acgaacgtgt tttccgtcat ggacgagaat tttctgaaag actgcacccg
     3541 cgcgcgcgca ctgctggccg aaatggaaaa acaccagcgg tggttcagtt tctacatttc
     3601 gtcatccgcc gaggcgattt ccgccttcgg cattgataat ctgatccggc tcggtgtgaa
     3661 catggtgtgg atcggctttg aatcgcagag cgccgacgag ttcgccaaga acgcggggat
     3721 caatgcggcc gaactggtca gggagcttcg tgaccggggc gtatccgtgc tgggctcggc
     3781 gatcatatgc atggagcatc acacgcctga aaacatcggc gcagacatag atttcgtggt
     3841 cggcctcggc acggacatga tgcagttcat gctctacacc ggcatgccgg taacgccgct
     3901 gtacacggat cataaagagc gcggcctgct gaaagaagat atgccctacg aggaatggca
     3961 cggacagaac gagctgaact ggaatcatcc ggcctttccc ggcgatgaac cgaagcgctg
     4021 gctggacctt gctttcaggc gtgattacga ggccaattca agctcgatct accggatggc
     4081 cgagaccgct gtgcgcggat acaagcggct ggcggcgatg gaatcaaggg acccctgcct
     4141 tgaggcccgc atgcagcagc tggcccttcg cgttcggctg tattcgcatc tgcttcccat
     4201 cattgcctgc cgcgcagtca atgagaagga gcgggaaaga gctcttgagc ttgatattga
     4261 gcagctcaaa ctgctcggcc cgctcaccgt gcgggatcgc atcacccgtc tggcggcaaa
     4321 agcgcttgcc gcgcgctggg atctccggct gcggctgttt ggcgaccgca tccagccttc
     4381 caccatcgtc acgcactacc acggcgggaa gcccgacaaa ggcctctgaa gaaacgaatt
     4441 cggcgtgcgc ttttgggtcc gacggggctt gacatatttt agccctctgg catactttta
     4501 caaaaatgta aaagttgttg aaccccatga gcaacaggaa ttcagagcag gtgcgggacc
     4561 ggctggtgcg gctggccggg acgatttcgc aggacctcgg gctgggacgc attaccggcc
     4621 aaatcctcgt ctatctctac ctccggccgg cggcctgttc gctggatgcg atcgaggccg
     4681 accttgggct gagcaaggcg gccgtcagca tcgccgcgcg tcagcttgag agtattggac
     4741 tgctgaagcg cattcgaaaa aagggcgacc gcaaaagcta ttaccagacg gcggagaatc
     4801 ttggaagcgc tctgcagaag ggcctgttcg gcctgatgtc ccgcaagctg gaaatagcca
     4861 acggtgaatt ccagcaggcc attcaggagc tggaagcgga gaaagcggac gcggacgcga
     4921 tctttctgga gaaacggatc aagcgggcaa tggtactggg caagcgcatg gacaagatca
     4981 tttccagtcc gctgattaaa ctcttgggac gatgataaat gagatttact gagcctggta
     5041 agcatattcg tcatttagtg ctgaagacca tgagggtgcc gcagagtcgc cgttatgact
     5101 ttatgctaaa cgaaggctgg aataaagact atatacataa ttggttgcag gcttactcct
     5161 attggaaaac catgcgggcc aaacagccta ggcttgtccg gattttcagc tattcaagga
     5221 gcggcaccca taattttttt tcacgttttc actatatgcc atgctgtttc gttattcgtg
     5281 aaaatatgtt tgagcaggtg gatgaccggc tgcagctgcg ggcgggcatt cattctttgc
     5341 gaccactgga catcatggcc ttttctgttt tcggccccta tggactgcag gataaatccg
     5401 cagcattgct gacacatgta tttttgccca gcaacagatt tctagaatat ccatgcctgc
     5461 tggcccctgg acagatgggt gatgacattg caattatgta cgtgcgcaat tatttcagga
     5521 tgctctattc acgccagaaa tcatcagaaa aatataacaa gccgaggttc aatataaccg
     5581 acgaagtgtt tgaaaactgg tcccgaagcc atcttgagaa gctgagcatt atggtaaacc
     5641 atataagaac cagacccgct cagtttatga tggttgttca cgaatatttc tgtgcccact
     5701 ccggagaagt tattgattct gtttgtgaaa ccatggggat tccgccgcat catagacaag
     5761 gatgggacaa cccggccggt ttttttaaga ggggatgggc atgcggcagt attccagatg
     5821 tgagggacgg caaaatatgg gacacccgtc gcggatgcca gatttgcgga acgggggaca
     5881 attataatcc gcttcctttg ccctcgcttg agagaaccat gggcgaccct gtcgcggatt
     5941 ggctcactcc tgcgcggctg tcagccgctc gttctgtctt cggccctgaa cttgtcgaat
     6001 tctggcttaa
//
LOCUS       DAOQWR010000142         5999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13508_length_5999_cov_5.661507, whole genome shotgun sequence.
ACCESSION   DAOQWR010000142 DAOQWR010000000
VERSION     DAOQWR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13508_length_5999_cov_5.661507"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="PKK36_10030"
     CDS             complement(<1..1862)
                     /locus_tag="PKK36_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR94928.1"
                     /translation="MKKYRYFIIGGLLLAALLLARNWSGMRRPPNLLLITMDTTRADR
                     LGCYAFPDALTPALDGLAAGGTLFEQAETCVPITLPAHTTILTGLYPPESGIRVNGAG
                     KLSPSIPLLSEILQLRGYRTGAFVAASVLHSQYGLNRGFDFYDDRDTLSPKNGDAHAE
                     LYRPANRVVDSALEWLSRHRLNNADGKPFFCWVHLFDPHTPLHSHADLFGKRFKNDYD
                     AEIAFMDIHIGRLLDFLKRNRLAGNTLVVAVGDHGEGLGEHGETTHGYMLYDSTLRVP
                     LIISRPGSIPAGWRVQGMVSLTGLFPTMLELLKVDPFAYGERNDPRNALVERILKNSF
                     APAVSSGESARADFYAETDHPYYNYGWSPLRALVTPEWKYIRAPEPELYDRTADPHET
                     RSLARENPSLVEQMDGRLADLEARMIRHASDAVSLTAEEMNRLQSLGYMAGGGGQVSE
                     GLGVRTDLADIKDRIGIVDLASEIDSRMKYEQGNSEQVLNMCRELIARSPDTPVFRLW
                     MGLLLANRKDYDAALESLDRAIQLFDEAIRKIPSDLRMVSYMSSAWDKKGLVLSMQKK
                     HEQAEKAFRLALAYEPENTDAHHHLGLALLELGRREEGLQSIREALRIDPDFELA"
     gene            complement(1859..3763)
                     /locus_tag="PKK36_10035"
     CDS             complement(1859..3763)
                     /locus_tag="PKK36_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94929.1"
                     /translation="MRQDSEKPDDDRRDGRHYPLIVFLIALLVRLIYLVSSSGNPAFF
                     DPVVDSNTYLDLARKVASGNAAGTSLFWQPVFYPVFLAAVFRFFGESIWLIKIIQAVI
                     GACSCLLIFQAARRVFSARTALASGLMMALYGPLIFYESELLGEGWAVFWSACMIYLF
                     ARAQEEDHPLYFMALGFCGGLGTLTRPPLLIVFVFGCAGFAWALSRRMEFRQALIRLG
                     LALICFAIVLHQPARICREQTGRYLLLPSSSGINLYIGNNKDWQRTVAARPGYEWRRI
                     YMMPRSAGVTNAWEESSYYQKLVHDYVKAEPGLFLKGLAAKTVRFFNSRELPRNIDIY
                     LFRKWSPVLSVLVWKAGPFGFPFGLLLPLAVLGLVLHWRRIPPAVWAFVLLYPAVIIL
                     YFVSGRYRILLIPALMIPAGSGLVHWVNLIAAGNRRRTAGLAAGFAVLMLLTSLPARF
                     PEEQVNFEAELYRSLGFQLHERQASAEAIPFYEKALELEPDLVTALNDMSNALTALGR
                     PREALAFQQKAVAVNPLEAGLHHNLGLLYLDEGRYPEAEAAFREALALETGIPNRNFY
                     LALSLMGRQRWEEASEFCAKELEINPEHKLARELQGEITKSVASGDLPTGMRLNIRGL
                     GTLKPQDVHP"
     gene            complement(3735..>5999)
                     /locus_tag="PKK36_10040"
     CDS             complement(3735..>5999)
                     /locus_tag="PKK36_10040"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNR94930.1"
                     /translation="ARISLLATALSGFLALALEVVWFRTLVLVFGSTTYSFCAMLSVF
                     LLGLAAGSLVFSRLIDRTRRPMLLFCLCLLCIGGYTLWSMTRFDAQPLFLLKHLMRHG
                     FSWSAMISAKFMITLFFLLVPTLLFGFMFPLAARMARGERHESAGVVGRVYACNTIGS
                     ILGSLAAGFVLLRFLGIAGSLMLLAGLSLACGAALMFLMRERHLLRAAVWIPALVLAG
                     LFSAFPPKLDPKLLIAGPYFTPWKYVQDGEIVLDKILASQKILLFEEGLLATIAVTKT
                     EAQELFYISDGKVEADSSPRSVMLQRMMGHLPMLFHPDPKRVMNIGLGAGMSFGALGC
                     YPVDALDVVEIEPASVLAADQWSDYNHNILQHPKTRIIIDDGRNYLLCTTSRYDVISS
                     DPFEPVHSGANNLYTVDHFSVAKSRLNPGGIMCQYLPLYELSMEDFRTIIRSFVSVFP
                     ESTFFYTGDDTILLGFKDQIRHAPEAVRAKLSIPEVQASLREIGMHRLESILGMFVAD
                     MTDLKDFAAAGSLNTDDRPVVEYSAPRSALHYTPDINMQVLLETLTPLPDSLVASLSE
                     EARASVDREREALQCILHANMLSSGGKVQEGAAMLRKAYELAPLNPVVRNDLVLMHTG
                     FGQSLLGAGRAGPAAEHFQQALMIQPNDFWALYHMVHLAMQAGQPQFAQNLIHHALGQ
                     YPESDVFYALNGKYEFTAGNAARAGLSYEKALSLAPEKAEYWEDYAAVAQAAGNVKLA
                     DEAREHAARLRKAR"
BASE COUNT         1286 a         1784 c         1870 g         1059 t
ORIGIN      
        1 gccagttcga aatcgggatc aatgcgcagg gcttcacgaa tggactgcag cccctcttcg
       61 cgccgtccca gttcgagaag cgccagcccc aggtgatggt gcgcatcggt attttcgggt
      121 tcgtaggcca gggcgagacg gaaggctttt tctgcctgct cgtgcttttt ctgcatgctc
      181 aggaccaggc cttttttgtc ccaggcgctg gacatgtagg acaccatgcg gaggtccgaa
      241 ggtatcttcc gaatggcttc atcgaagagc tggatggccc ggtcaagact ctcaagcgcc
      301 gcatcatagt ctttccggtt ggccagcagc agtcccatcc acaggcggaa aacgggcgtg
      361 tccgggctcc gggcgatcag ctcgcggcac atgttcagca cctgttcact gtttccctgc
      421 tcatacttca tccgggaatc aatttcagag gccagatcca caatcccgat gcggtccttg
      481 atgtctgcaa ggtccgtccg cacaccaagg ccttcggaca cctgcccgcc gccgcccgcc
      541 atatagccga ggctttgcag gcgattcatt tcctctgccg tcagggatac cgcatccgag
      601 gcatggcgga tcatgcgggc ttcgaggtct gccagccggc cgtccatttg ttcaaccagc
      661 gagggattct cccgtgccag gctgcgggtc tcatggggat cggcggtgcg atcgtacagt
      721 tcaggttcgg gcgcccggat atatttccat tcgggggtga ccagggcgcg cagcgggctc
      781 cacccgtaat tgtaataggg gtgatccgtc tccgcgtaga agtcggcacg tgcggactcg
      841 cccgatgaaa cagccggcgc gaagctgttc ttcaggatgc gctcgacgag ggcgttgcgg
      901 ggatcgttcc gttcgccata tgcaaaagga tccaccttca gcagttcaag catcgtgggg
      961 aacaaacctg tcagcgaaac catgccctgc acgcgccagc cggcgggaat tgatcccggc
     1021 cgggaaatga tcagcggcac ccggagcgtg gagtcataaa gcatgtagcc gtgcgtggtt
     1081 tcgccgtgtt ctccgagacc ttcgccgtga tcgcccaccg ccaccaccag ggtgtttccg
     1141 gcaaggcggt ttcgtttcag gaaatccagc aggcggccga tatggatgtc catgaaggcg
     1201 atctcggcat catagtcgtt cttgaaccgt ttcccgaaaa ggtctgcatg gctgtgaagc
     1261 ggggtatgcg ggtcgaaaag atgcacccag cagaagaaag gtttcccgtc cgcattgttc
     1321 agccggtggc gcgacagcca ttccagggcc gaatccacaa cccggttcgc ggggcggtaa
     1381 agttccgcat gggcgtcgcc gtttttcggc gacagcgtgt ccctgtcgtc gtagaaatcg
     1441 aaaccccggt tcagtccata ttgcgaatgc agaacactgg cggcgacaaa cgcgccggtg
     1501 cggtagcccc gcagctggag aatctcagac agcaggggga tggacggact gagttttccc
     1561 gcgccgttga cccggatgcc gctctcggga ggataaagtc cggtcagaat ggtcgtatgt
     1621 gcgggaaggg taatggggac gcaggtttcg gcctgctcaa aaagagttcc tcccgcggcc
     1681 agcccatcca gcgccggggt cagcgcatcg gggaaggcat agcatcccag ccggtcggcc
     1741 cgtgtcgtgt ccattgtgat cagcaggaga ttcggcggcc gtcgcattcc ggaccagttc
     1801 cgcgcgagca gcagcgccgc gagcaggaga ccgccgatta tgaagtatct gtatttcttc
     1861 atggatgcac atcctgcggc tttaaagtcc caaggccccg gatgttgagc cgcatgcccg
     1921 tcggcaggtc gccggatgcg accgatttcg tgatttcccc ctgaagctcg cgcgccagtt
     1981 tatgttccgg gtttatctcc agctccttcg cgcagaattc agaagcctcc tcccagcgct
     2041 ggcgtcccat caggctgagc gcgagataga aattccggtt gggaatgccg gtctcgagcg
     2101 caagggcttc cctgaatgcg gcttcggcct cgggatacct gccttcatca agataaagca
     2161 ggccgaggtt gtgatgcagt cccgcttcga ggggattgac ggccacggcc ttctgctgga
     2221 acgccagagc ctcccgggga cggcccagcg ccgtgagggc gttgctcatg tcgttcaggg
     2281 ccgtgacaag atccggctcc agctccagcg ccttctcgta gaacggaatg gcctcggccg
     2341 aggcctgccg ctcatgaagc tgaaatccga gcgaccggta gagctcagcc tcgaagttga
     2401 cctgttcctc cgggaaccgc gccggaagcg aggtcaggag catgagcacg gcgaagccgg
     2461 ccgcgagccc ggcggttcgg cgccggttcc cggccgcgat caggttcacc cagtgaacca
     2521 gccccgatcc ggccggaatc atcagcgcgg gaatcagcag gatccggtag cgcccggaaa
     2581 caaaatagag aatgatcacc gcgggataga gaagaacaaa ggcccagaca gccggcggaa
     2641 ttctgcgcca gtgcagaaca agcccgagaa cggccagggg cagcagcagg ccaaacggga
     2701 agccgaaggg tccggctttc cagaccagga cagagaggac gggggaccac ttgcggaaaa
     2761 ggtaaatgtc aatgttgcgc ggcagttcgc gggaattgaa gaaccggacc gtttttgcag
     2821 ccagcccctt gagaaagagc cccggttctg ccttcacata atcatggacc agtttctggt
     2881 aataggagga ttcctcccac gcattggtga cgccggcgct gcggggcatc atgtagatcc
     2941 tgcgccactc gtagcccggg cgtgccgcca ccgtgcgctg ccagtccttg ttgttgccga
     3001 tgtacagatt gattccggag cttgaaggca gcagaagata gcggccggtc tgctccctgc
     3061 atatccgcgc gggctgatgc agaacgatcg caaagcagat cagcgccagc ccgaggcgga
     3121 tcagggcctg ccggaactcc atgcggcggc tgagcgccca tgcgaacccg gcgcagccaa
     3181 aaacaaaaac aatcagcagg ggcggccgtg tcagagtgcc caggccgccg cagaaaccga
     3241 gagccatgaa atacagcgga tggtcttcct cctgcgccct tgcaaagaga taaatcatgc
     3301 aggcggacca gaacaccgcc cagccttcgc ccagcagttc ggattcataa aaaatcaggg
     3361 gcccgtagag cgccatcatc aggcccgacg cgagggccgt ccgcgcggaa aaaacgcgcc
     3421 gcgcggcctg aaagatcagc aggcatgagc aggcgccgat cacggcctgt atgatcttga
     3481 tcagccagat ggactcgccg aaaaagcgga acaccgcggc gagaaaaaca ggataaaaaa
     3541 cgggctgcca gaaaagcgaa gtgcccgcgg catttcccga agccaccttg cgggcgagat
     3601 ccaggtaggt gttggaatcc accaccggat caaaaaatgc gggatttccg gacgatgaaa
     3661 ccagatagat gagccggacc agcagggcga tcagaaaaac aatcagcggg taatggcgcc
     3721 cgtcccggcg gtcatcatct ggcttttctg agtcttgccg catgttccct ggcttcatcc
     3781 gcgagtttca catttcctgc cgcctgcgcg accgccgcat aatcttccca gtactccgct
     3841 ttttccggcg cgaggctcag cgccttctca taggagagcc cggcgcgcgc ggcgtttccc
     3901 gccgtgaatt catacttccc gttcagcgca tagaatacat cggactcggg atactggcca
     3961 agcgcatgat ggattaaatt ttgcgcgaac tgcggctgcc cggcctgcat ggccagatga
     4021 accatgtgat acagggccca gaagtcgttt ggctggatca tcagcgcctg ctggaaatgt
     4081 tcggcggcgg ggccggcccg gccagcgcca agcagtgact ggccaaagcc ggtatgcatc
     4141 agcaccaggt cgttgcggac cacgggattc agcggggcca gttcataggc cttccggagc
     4201 atggcggcgc cctcctgcac ctttcccccc gaactgagca tattggcgtg aagaatgcac
     4261 tgcagcgctt cgcgctcgcg atccacggac gcgcgggctt cctcgctcag gctggccacc
     4321 agagagtcgg gcaggggcgt cagggtttcc agaaggacct gcatgttgat gtcgggcgtg
     4381 taatgcaggg cgcttcgcgg cgcggaatac tcgaccaccg gccggtcgtc cgtgttcagg
     4441 gacccggccg cggcgaaatc cttcaggtcg gtcatgtccg ccacaaacat gccgagaatg
     4501 ctttccagcc ggtgcatgcc gatctcgcgc agagaggcct gcacctccgg gatggacagc
     4561 ttggcccgca cagcttcggg cgcgtgccgg atctggtcct tgaacccgag aagaattgtg
     4621 tcgtcgcccg tatagaaaaa agtgctctcg ggaaagacgc tgacaaacga gcggatgatc
     4681 gtgcggaaat cctccatcga aagctcgtag agcggcagat actggcacat gatgccgccc
     4741 gggttcagcc gcgatttcgc cacggaaaag tggtccaccg tgtagagatt gttggccccg
     4801 gaatgcaccg gctcaaaggg atcggacgaa atcacatcgt accgggaggt ggtgcagagc
     4861 agataatttc ttccatcgtc aataatgatg cgcgtcttcg ggtgctggag gatattgtgg
     4921 ttgtagtccg accactgatc cgccgccagc accgaggccg gttcgatctc cacaacatcg
     4981 agcgcgtcca ccggatagca tccgagcgcg ccgaagctca tgcctgcgcc gagcccgatg
     5041 ttcatcaccc gcttgggatc gggatgaaac agcatgggca gatgacccat catacgctga
     5101 agcatcacgc ttcgcggcga ggaatcggcc tccaccttgc cgtcgctgat gtagaacagc
     5161 tcctgcgcct cggtcttcgt cacggcaatc gtggccagca ggccttcctc gaacaggagg
     5221 atcttctgcg aggcgaggat tttatccagc acgatctctc cgtcctgcac atacttccac
     5281 ggcgtgaaat aggggccggc aatcagcagt ttcgggtcca gcttcggagg gaacgcggaa
     5341 aagagtccgg cgaggacaag cgcgggaatc catacggctg cgcggagaag atgcctttcg
     5401 cgcatcagga acatcagcgc cgcgccgcag gccagggaaa gtccggccag aagcatcagc
     5461 gatccggcaa tcccgagaaa acggagcagc acaaagcccg ccgccagcga accgagaatg
     5521 gaaccaatgg tgttgcaggc gtagacccgg cccacgacgc ctgcggattc atggcgttcg
     5581 ccgcgggcca tgcgggccgc cagcgggaac atgaacccga acagaagcgt tgggaccagg
     5641 aggaaaaaca gcgtgatcat gaacttggcg gagatcatgg cggaccagga gaacccgtgg
     5701 cgcatcaggt gcttcagcag gaacagcggc tgcgcgtcga aacgggtcat ggaccagagg
     5761 gtatagcccc cgatgcacag caggcagagg cagaacagaa gcatgggccg gcgagtacgg
     5821 tcaatcagtc ggctgaagac gagcgatccg gcggcgagtc cgagcaggaa aacggaaagc
     5881 atggcgcaga aggaataggt ggtggatccg aaaaccagaa ccagtgtccg gaaccagacc
     5941 acctcgagtg cgagcgcaag aaaacccgac agggccgtcg ccagcagact gatccgggc
//
LOCUS       DAOQWR010000143         5998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13510_length_5998_cov_5.309608, whole genome shotgun sequence.
ACCESSION   DAOQWR010000143 DAOQWR010000000
VERSION     DAOQWR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13510_length_5998_cov_5.309608"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /gene="cysS"
                     /locus_tag="PKK36_10045"
     CDS             complement(<1..1278)
                     /gene="cysS"
                     /locus_tag="PKK36_10045"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR94931.1"
                     /translation="MSMKIFNTLSRTAEELVPLEGNHVRMYTCGPTVYNFAHIGNFRA
                     YMFEDLLRRYLRFRGFRVTQVMNLTDVDDKTIRGCRAAGLPLREYTQKYKDAFFSDLK
                     TLSIEPAEHYPAATDHVPAMIALIEKLLEKGFAYRSDDGSVYFSIAKFPGYGKLAHLD
                     LSGLRAGARVAHDEYEKENLADFALWKAWDEEDGDVVWDAPWGRGRPGWHIECSAMSM
                     HYLGDSFDLHTGGMDNIFPHHEDEIAQSEAATGKPWVKYWMHCAHLIVDGKKMSKSAG
                     NFFTLRDILDRGWSGRDIRYVLLTTHYRQTLNFSFDSLEAAHAALQRVDEFTARLKDR
                     AGQAAASAELPDWAREAVGKFTAALDDDLNISGGMAAVFDMIHAGNRAMDRDELSAGE
                     ARRALDEMASLDQVLGFLTAAEDEIPAEVTALVE"
     gene            1522..2220
                     /locus_tag="PKK36_10050"
     CDS             1522..2220
                     /locus_tag="PKK36_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR94932.1"
                     /translation="MPAARRRVFCTVAVSALLLCLSGLACSAEIPPEAQGCPNFKSPL
                     ADVTFVAFDTETTGYAGGDHRIVELGAVKFRNGEIIEEKTWLINPERYIPYFARQVHH
                     ISNDMVKNSPTFAQVYPEFMEFIDGAVLVAHNAPFDVGFIRDEVIRNSLSVPTNPVIN
                     SLNLFRSWFPEAGSHSLEPLTEHLDLPKGTYHRATDDSRYLAEIIHHKVQNRKGRQMT
                     FGDVYKAAHGVVRF"
     gene            2346..3638
                     /locus_tag="PKK36_10055"
     CDS             2346..3638
                     /locus_tag="PKK36_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94933.1"
                     /translation="MIGTFPAGISSRWRKAGGMENAAYYKQTPIMRRILEYTDGAVSI
                     SGTNPLKKDDKFFRRYDDILEAMGDGCDLYRSMLDFKGPLLALDLEYGNSAHPGEIFH
                     KTLETFQKFEPTRLAARNILNEHGIEHVELMTGQGYNYVMRVPRTSPTYKSLLTIGSK
                     LHVLNRSTLDALEQKERQFGNVPLVTDSIVFAAFGRVTDYLVEEIKTRSELLLRTTDL
                     FDSEEIAIFDTTLYGYLFNRRSIRTAFSLHQKTRMKEIYNYDGPAIVAIPVAGVPLEE
                     RLKIRQDEREHYRRAVQFADEVRCEIPAANLGSLLVTYMTSPVYDRHLDFSRQLQRED
                     PNRWNLPEWPELEAKMRGAGLDQETLDILAMPNPQLLQPWWLRHVIRSLREHGFSFAE
                     VVGMITTKYNAQFNWHEDLVKSNSALRAEYWTRTLWEV"
     gene            3653..4768
                     /locus_tag="PKK36_10060"
     CDS             3653..4768
                     /locus_tag="PKK36_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNR94934.1"
                     /translation="MKENVYWMKFDVLKPFMVDVFKGMGVPADEAEICAEVLIEADRR
                     GIDSHGVGRLKTIYYNRVVKDRIQNARTDFQVLKDRMATAVVDGNQGMGMVIAHRCME
                     MAIEKARTYGVGVVTARNSTHYGIAAYYPMMAARAGMIGMTATNARPSIAPTFGVENM
                     LGTNPLVYAMPTDEDFPFTNDYATSISQRGKIEAYARNGKDLPRGWVIGRDGESKTNA
                     KAVLDDLTHDRAALAPLGGIGEETAGYKGYGFATVAEILAASLQQGDFLKRLSGTDEN
                     GQKAPIRLGHFFMAVNIEFFTDLEKFKKTTGDILRELRASARAPGEKRIYTCGEKEYL
                     AWLERKDKGAPLGEVLQKELIAMRDELGLSQYKFEFE"
     gene            4773..5549
                     /locus_tag="PKK36_10065"
     CDS             4773..5549
                     /locus_tag="PKK36_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR94935.1"
                     /translation="MNFFVPAKDNTKLAALLKAINADAELMQLWRCANTNAVSRCGIS
                     DHGEIHIQIIANAALKLLRLLVAGGIAPSVVEHHKLTAHEAEIIVVLAACLHDVGIVI
                     HRHDHEKYSPIIAYPKCRQLLEGICSEPEMTIIVSEVLHAIVSHNANEECLTIEAGVL
                     KVADALDMSKGRSRIPFEAGDVNIHSVSAQAVDDVVITRGETRPVQIDITLTNSAGIF
                     QVDELLRKKLKNSTLAPYVEVAARLKEGEGERRILEMVRL"
     gene            complement(5565..>5998)
                     /locus_tag="PKK36_10070"
     CDS             complement(5565..>5998)
                     /locus_tag="PKK36_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR94936.1"
                     /translation="RLLTLLKTENVLETDLTELPPSMTLGELVKVISASKRNIFPVVH
                     PETRELLGVVLLDEIRSIMFEPDLYGKFTVEKLMNAPPATIRKDSSMEKVMRTFEETG
                     AWNLPVVDWQKRYVGFVSKSKIFSLYRHVLVTFANIEQGEN"
BASE COUNT         1293 a         1801 c         1747 g         1157 t
ORIGIN      
        1 ctcaaccagc gccgtcactt ctgcgggaat ttcatcctcg gcagccgtga gaaaaccaag
       61 cacctggtcc agactagcca tttcgtccag tgcccgacgg gcttccccgg cggacagctc
      121 atcccggtcc atcgcccggt tccccgcgtg aatcatgtcg aacaccgccg ccatgcctcc
      181 ggagatattg agatcatcat ccagcgccgc ggtgaatttc ccgaccgcct cgcgcgccca
      241 gtccggaagt tcggcagacg cggcggcctg ccccgccctg tccttgagcc gcgcggtgaa
      301 ttcgtccacg cgctggagcg cagcgtgcgc cgcctcgagg ctgtcgaaac tgaaattcag
      361 ggtctgccga taatgagtcg tcagcagcac ataacggata tctcttcccg accagcccct
      421 gtcgaggata tcccgaagcg tgaagaaatt ccccgccgac ttggacattt tcttcccgtc
      481 cacgatcagg tgcgcgcagt gcatccagta tttcacccac ggcttgccgg tcgcggcctc
      541 gctctgcgcg atttcatcct cgtggtgcgg aaagatgttg tccatcccgc cggtatggag
      601 atcaaaactg tcgcccagat agtgcatgct catggcggag cactcgatat gccatccggg
      661 tcttccccgc ccccagggcg cgtcccacac cacatcaccg tcctcttcgt cccacgcttt
      721 ccagagggcg aaatcggcca ggttctcctt ttcatactca tcgtgcgcca cgcgcgcgcc
      781 cgcgcgcagg ccggacaggt ccagatgcgc cagcttcccg tagcccggga acttcgcaat
      841 gctgaaatag accgatccgt cgtccgaccg gtaggcgaag cccttctcca gcagtttttc
      901 gatcagtgcg atcatggccg gcacgtgatc cgttgcggcg ggatagtgct cggccggctc
      961 gatgctgagc gttttgagat cggagaaaaa ggcgtccttg tacttctgcg tgtactcgcg
     1021 caggggcagc ccggccgcgc ggcagccgcg gatggtcttg tcgtccacat cggtcaggtt
     1081 catgacctgc gtaacccgga acccgcgaaa ccggagatag cggcggagca gatcttcaaa
     1141 catataggcg cggaagttgc cgatatgggc gaaattataa accgtcggcc cgcaggtata
     1201 catgcggaca tggttcccct ccagcgggac cagttcctcc gccgtgcggc tcagcgtgtt
     1261 gaaaatcttc atgctcatat tccgtgctcc ggcctcagcc actcaaaaag cgcagattat
     1321 accgatcagc gcccgccgtt caatgtttca ttccccgaaa agcagccggt aaaaactttt
     1381 acctgcgcta ttgaccccga cggcaagcgg gggtagtctt tctgtcaggt aaacacgcag
     1441 gcagactgag ttagtataaa atatttaagg agtattcggc tgttccgaat atttttgatg
     1501 ctggttatga gaataatagt catgcctgcg gcccgtcgcc gcgttttctg caccgttgcc
     1561 gtgtctgcgc tcctgctctg cctgtcaggc ctggcctgct cggctgaaat tcccccggaa
     1621 gcacagggct gcccgaattt caagagccct cttgcggatg tcacctttgt cgcattcgac
     1681 accgaaacca cgggctatgc gggcggagat caccgcatcg tcgaactggg cgccgtgaaa
     1741 ttccggaacg gggaaatcat cgaggaaaaa acctggctga tcaatcccga gcgctacatc
     1801 ccctactttg cgcggcaggt gcatcacatc agcaacgaca tggtgaagaa ctcgccgacc
     1861 ttcgcgcagg tctatcctga attcatggaa ttcatagacg gggccgtgct tgtggcccac
     1921 aacgcgccgt tcgacgtggg ctttatccgc gatgaagtca tccggaacag tctctctgtt
     1981 cccaccaatc cggtcatcaa ctcgctcaat cttttccgga gctggttccc ggaggcggga
     2041 agccactccc tcgagccgct gacagagcat ctggatctgc caaagggaac ctatcaccgc
     2101 gcgacggacg actcgcgcta ccttgcggaa atcattcatc acaaggtcca gaaccgcaag
     2161 ggccgccaga tgacctttgg cgacgtctac aaagccgccc acggcgttgt ccgtttctga
     2221 tccgcgccgg ccgccggcgt tttaaacttc caatcactgg aagttttggg ataggatttt
     2281 ccaatcattg gaagttttca gtgaaaaagt tccaatggct ggaagttttt acgcgaaaag
     2341 ttccaatgat tggaactttt cccgccggga tttccagccg ctggagaaag gcgggcggaa
     2401 tggagaatgc ggcatactac aagcagacgc cgatcatgcg gcggattctc gaatacaccg
     2461 acggggccgt ctcgatttcc ggaacgaatc cgctgaagaa ggacgacaag ttcttccggc
     2521 gctatgacga tattctcgaa gccatgggcg acggctgcga cctgtaccgg agcatgctgg
     2581 atttcaaggg gcccctgctc gcgctcgacc tggaatacgg aaactcggcg caccccggcg
     2641 agattttcca caagaccctc gaaacgttcc agaagttcga accgacacgc ctcgccgcgc
     2701 gcaacatcct gaacgagcac ggcatcgagc atgtcgaact gatgacgggc cagggctaca
     2761 actatgtcat gcgcgtgccc cgcacctcgc cgacctacaa gagcctgctc acgatcggga
     2821 gcaagctgca tgtgctcaac cgctccacgc tggacgcgct ggaacagaag gagcggcagt
     2881 tcggcaacgt gccgctggtg accgacagca tcgtgtttgc cgcgttcggc cgcgtgacgg
     2941 attacctggt ggaggagatc aagacgcgct ccgagctgct gctgcgcacg acagaccttt
     3001 ttgacagcga ggagatcgcg atcttcgaca cgacccttta cggctatctg ttcaatcgcc
     3061 ggtctatccg aacggcattt tcactgcacc agaagacgcg gatgaaggag atctacaact
     3121 atgacgggcc ggctattgtc gcgatccccg tcgcgggtgt gccgctggag gagcggctga
     3181 agatccggca ggacgagcgc gagcactacc ggcgcgccgt gcagttcgcc gacgaggtgc
     3241 ggtgcgaaat cccggccgcc aatctgggga gcctgctggt cacctacatg acatcgccgg
     3301 tgtacgaccg ccatctcgat ttcagccgcc agctccagcg cgaagacccg aaccgctgga
     3361 atctgcccga atggcccgaa ctggaggcca agatgagagg cgcagggctc gatcaggaaa
     3421 cgctggatat cctcgccatg cccaatccgc agctgctcca gccgtggtgg ctccggcacg
     3481 tcatccgcag cctgcgggag cacggtttca gtttcgccga ggtggtgggc atgatcacca
     3541 cgaaatacaa cgctcagttc aactggcacg aggatcttgt caagagcaat tcagccctgc
     3601 gtgcggagta ctggacccgc acgctgtggg aagtctgaac aggagaagac gcatgaaaga
     3661 gaatgtttac tggatgaagt ttgacgtgct gaagccgttc atggtggatg tgttcaaggg
     3721 catgggggtg ccggcggacg aagcggaaat ctgcgcggag gtgctgattg aggccgaccg
     3781 gcgcggaata gactcgcacg gcgtcgggcg gctcaagacg atttattaca accgcgtggt
     3841 gaaggacaga atccagaacg cccggacaga tttccaggtg ctgaaggacc gcatggcgac
     3901 tgccgtggtg gacgggaacc agggcatggg catggtgatc gcgcaccgct gcatggaaat
     3961 ggcgattgag aaggcccgca cgtacggggt cggagtggtc accgcccgca attccacgca
     4021 ctacggcatc gccgcctatt atcccatgat ggcggcgcgg gccggaatga tcggcatgac
     4081 ggcaacaaat gcgcggccgt ccatcgcccc caccttcggc gttgaaaaca tgctgggcac
     4141 caatccgctg gtctatgcca tgcccaccga cgaggacttc cccttcacca acgactatgc
     4201 cacttccatt tcacagcgcg ggaagattga agcctacgca cgcaacggga aggatctgcc
     4261 gcgggggtgg gtgatcggcc gggacggcga gtcaaaaacc aatgcaaagg ccgtgctgga
     4321 cgatctgacc catgaccgtg cggcgctggc gccgctcggc ggcatcggag aggaaaccgc
     4381 cggctacaag ggctacggct ttgccacggt ggcagagatt ctggcggcat cgcttcagca
     4441 gggcgacttc ctgaaaaggc tttccggaac cgatgaaaac gggcagaagg ccccgatcag
     4501 gctgggacat ttcttcatgg cagtgaacat tgaatttttc actgatctgg aaaaattcaa
     4561 gaagaccacc ggcgacatcc tccgcgagct gagggcctcc gcccgggcgc ctggcgagaa
     4621 gcgcatttac acgtgcggag aaaaggaata ccttgcatgg cttgaacgaa aggacaaggg
     4681 cgccccgctg ggcgaagtgc tccagaaaga gctgattgcc atgcgcgacg aactgggcct
     4741 ttctcaatac aaattcgaat tcgagtgaaa ctatgaactt ttttgttccc gccaaggaca
     4801 acaccaaact cgcggcgctt ctgaaggcta tcaacgccga tgcggagctg atgcagctct
     4861 ggcgctgcgc caacaccaac gcggtcagcc gctgcggcat cagcgaccac ggggaaatcc
     4921 acatccagat catcgccaac gcggcgctga agcttctgcg cctgctggtc gcgggcggca
     4981 tcgctcccag cgtcgtggaa caccacaagc tcaccgccca tgaggccgag atcatcgtgg
     5041 tgctggcggc ctgcctgcac gatgtcggga tcgtcattca ccggcacgat catgaaaaat
     5101 acagcccgat tatcgcctac cccaagtgcc ggcagcttct ggaaggcatc tgcagcgaac
     5161 cggaaatgac catcattgtc agcgaggtcc ttcacgcgat cgtcagtcac aatgccaacg
     5221 aagaatgcct gaccatcgag gccggcgtgc tgaaagtggc cgatgcgctg gacatgagca
     5281 agggccggtc acggatcccg ttcgaggccg gcgacgtgaa catccattcc gtttccgcac
     5341 aggccgtgga cgatgtggtc atcaccagag gggaaacgcg gccggtgcag atagacataa
     5401 cgctgaccaa ctcggcgggg attttccagg tggatgaact gctgcgcaag aagctcaaga
     5461 attcaacgct ggcgccgtat gtggaagtgg ccgcccgcct gaaggagggc gaaggcgaac
     5521 gccgcattct tgaaatggtc cgcctgtagc cggtacaggc gcggttagtt ttcgccctgc
     5581 tcgatgttcg caaaagtcac cagaacatga cggtacaggc tgaatatctt tgattttgac
     5641 acgaagccga catagcgctt ctgccagtcg acgaccggga ggttccacgc gcccgtctcc
     5701 tcaaaggtcc gcatgacttt ctccatggag gaatcctttc ggatcgtggc gggcggagcg
     5761 ttcatcagtt tttcaaccgt gaatttcccg tacaggtcgg gctcaaacat gatgctgcgg
     5821 atttcgtcga gtagcaccac gcccagcagc tcccgggttt ccggatgcac caccgggaat
     5881 atgttgcgct tcgatgcgga aatcaccttc acaagctcgc cgagggtcat ggaaggaggc
     5941 agttcggtca aatccgtctc cagcacgttc tcggtcttga ggagcgtgag caatcgga
//
LOCUS       DAOQWR010000144         5926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13728_length_5926_cov_5.910066, whole genome shotgun sequence.
ACCESSION   DAOQWR010000144 DAOQWR010000000
VERSION     DAOQWR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13728_length_5926_cov_5.910066"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKK36_10075"
     CDS             complement(<1..326)
                     /locus_tag="PKK36_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94937.1"
                     /translation="MDQNQTQDRFRGFCPMCQAEANLTRQHGKADLTVDDQIVTVDVE
                     YLKCLDCGETFDDPLSGDPLIGIYEQIGRGKKPKPAAKPASDVGDYLGLMFFAFATIM
                     AGIAAW"
     gene            complement(329..1192)
                     /locus_tag="PKK36_10080"
     CDS             complement(329..1192)
                     /locus_tag="PKK36_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94938.1"
                     /translation="MSGLIIVVGVLAAILILVVTMEYVPTMRRGKAAQALAESSVQTD
                     DSITGLRRALIPMEKPVQKYAGPKMLKKAWYDLYWAQIVDKYVGWTALQFVTVRVFAA
                     MVGAVVGLVVFGNPIFMAAVAFMGWQYPAMGLGGIARRARRQFQSQLPEYINLVAAQM
                     SAGISMEEALRRTSRSASLPAKWMRRVIQMAQGRTLVGQIVRESEESQLPELISMGVQ
                     LGFIQRGAGQQLLLAQLSTQIAADYIGQTEMRAEKIGGELVIPMVLFYFLPFMVSLMM
                     VIAWPIVVGML"
     gene            complement(1218..2111)
                     /locus_tag="PKK36_10085"
     CDS             complement(1218..2111)
                     /locus_tag="PKK36_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94939.1"
                     /translation="MTVYLIGILAAALIWLLFGPKMGGMKKGKATEALSDAYSLEQTR
                     KLSEMNPDSLEYKLAASGVNWRPATFKWGRILLGLGGALVVWTVLPGVPALAIGGLLF
                     YAPMAWLTDKVKGRGREIDKELPIAIGRISAGLLAGGSMADVLDEVAVSMGLQGKSPL
                     SGELHLTAAELRSKERSEAFQNLAQRSPSLSLSNLAYLLEGYLESGGSKNNQVLIESA
                     TRVQQILVARNRTKAKAGDAMLSAKVIPGVLAIVLIYLAQDPMVKQSLMVIPVQIVLG
                     LTIGAMVMGYFIMRSMVMEAA"
     gene            complement(2127..3413)
                     /locus_tag="PKK36_10090"
     CDS             complement(2127..3413)
                     /locus_tag="PKK36_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR94940.1"
                     /translation="MSLLSVFDDKQTAQQAVSGDLRIEVSTEVIDVINREFPPALLQR
                     PNEIERQRVAERIASLVTQALRRRSANPGQGFEVELAGELARRLMGLGFLDLLLPPSR
                     NDLSEITVYSSGLVQIMRKGEVRWETIPMRPQAEEVERVLDRLLGPQNKSLNETNPSV
                     NAKLPRTEDNPGGGRVKALHRVITPPGRNHSINIRLYEQKPVLPEWLIERQALSADMM
                     DTLRRGMASGQRILITGGTRTGKTTLLSAICNFLPPAWRVLKIEDPEEIWIARDTVQT
                     IEARPRAVGTEVQPYTLADGVDDAMRMSPDYLIIGEVRDGRAAQALFRAMMTGHSGAC
                     TFHADSPREAASRLATIMGADAGVRKVDANQMVADSVDMLVQIGIRHEKRCVTTIAQI
                     ARELRGGDVWFDPLWQYDETSQPGAPRWEKLAELEE"
     gene            complement(3410..3832)
                     /locus_tag="PKK36_10095"
     CDS             complement(3410..3832)
                     /locus_tag="PKK36_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94941.1"
                     /translation="MDTIIQIVHLPDYAAVFECVNDEAGTAPESAGILARVDARVRSL
                     PLLCYALVRSETPHAETPVYRMVGVILDPDLGYLDLCERTDGEEVRVGRFLGYLPPDK
                     TTSASLMNNYRQIVAAGLDGRYAEDATWNQVIASEEEK"
     gene            complement(3832..5136)
                     /locus_tag="PKK36_10100"
     CDS             complement(3832..5136)
                     /locus_tag="PKK36_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR94942.1"
                     /translation="MLNQEAKVLTMLAGTGAETVFYQMMPAFQSDTRFSVSSVVTGWS
                     DFEKNLEQLKPNLLVVQGDIAPNPDTLIAALARMQVWNGVALVVLQAAHREFRGAFEK
                     VSVVRGVFVLPVNWGEVVQGGYAAVSTERARGAAQAPLQSAMGFRAATSVTGTRVVAF
                     LSGSGGAGRSTLAENIAYELAVRLNIRTLLMSLGLPPSAAMNLGLKYTPNAGEFFARP
                     GDGFASAIQTKEGLDVIVAPENSVEYARAAESSSSAGGKAEANSIYSLVMASWTRSYA
                     GVMLDLPCSEGIWTMHPLMAANTAIIVSRPTLADLAATRHLLVLLMERMRNEHRIPQE
                     SIFMVLNQASDRSRITPSQFYQELVQSYGWAPPVLAVIPYDQNIPNTQDDRVPVVGKV
                     DGFTKGIRTIINGLFPGVAGQTTSDSSQKKGFSLGSLLGGRG"
     gene            complement(5159..>5926)
                     /locus_tag="PKK36_10105"
     CDS             complement(5159..>5926)
                     /locus_tag="PKK36_10105"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HNR94943.1"
                     /translation="VQRAAGDAITASTLGDNAQAGIPAQLEPGHVAIAVNVDLSSGVA
                     GLLRPGQTVTIIGLLTPDVVGSQSGLSSVIPAASVLVPDLSADPNSLLTPTPTATPIP
                     LMGPVGRISISGLRVLMVPQSFQYQEIPAGASQEELFASSAATSKDSGVIVLDVPTTP
                     MEIAPGVFVNPATLIAALDQFGVVYLALEPSSGLVVAEEDILTLNLASLYKSINGNNQ
                     VSAAATPTTYYHPTATPEPVATATPEPTLLPTLAPKP"
BASE COUNT         1200 a         1881 c         1645 g         1200 t
ORIGIN      
        1 tcccaggctg caatcccagc catgatcgtt gcaaaggcaa aaaacatcag ccccagataa
       61 tccccaacgt cagaggccgg ctttgcagcc ggcttcggtt tcttccctcg tccgatttgc
      121 tcgtagatgc caatcagggg gtcgccagac aggggatcgt caaaggtttc accgcagtcc
      181 aggcacttca agtattcaac atctaccgtg acaatctgat catccaccgt caggtcggct
      241 ttcccatgct gccgggtcaa attggcctcg gcctgacaca tcgggcaaaa tccacgaaaa
      301 cgatcttgag tctggttctg gtccattttt acagcatccc cacgacaatc ggccaggcaa
      361 tgaccatcat caggctgacc ataaagggca ggaagtagaa caacaccatc gggatcacca
      421 gttcaccgcc aatcttctca gcccgcattt cggtttgccc gatatagtcg gctgcgattt
      481 gggtggacaa ctgcgccagc aaaagctgct gcccggcgcc gcgctggata aagcccaact
      541 ggacgcccat gctgatcagt tcgggtaact gcgattcttc ggactcccgg acaatctgcc
      601 cgaccagggt gcgcccctgc gccatttgga tcacccgccg catccacttt gccggcaggc
      661 tggccgagcg ggaggtgcgc cgcagggctt cctccatcga gatacccgct gacatttgcg
      721 ctgcaaccag gttgatgtac tccggcaact gtgactggaa ctgccggcgg gcgcgccggg
      781 caattccacc cagacccatt gctggatact gccagcccat aaaggccacc gctgccatga
      841 aaatcggatt gccaaacacg accaggccga ccaccgctcc aaccattgcc gcgaacacac
      901 gcacggtcac aaattggagc gccgtccagc caacatattt atccactatc tgcgcccagt
      961 agagatcgta ccaggctttc ttcaacatct ttgggccggc gtatttctgc accggttttt
     1021 ccattggaat caatgcccgc cgcagcccgg tgatgctatc atcggtttgg acgctgcttt
     1081 cggcaagcgc ctgcgctgcc ttgccccggc gcatggtggg aacatactcc atcgtgacga
     1141 ccagaatcag gatcgccgcc aggacaccaa caacaataat caatccactc atatttcgta
     1201 tctccttcct tactgtatta agcagcttcc atgaccatcg agcgcatgat gaagtagccc
     1261 atcaccatcg caccaatcgt caggcccagt acaatctgca ccggaattac catcaggctt
     1321 tgtttcacca tcgggtcctg cgccagatag atcagcacaa tcgccaacac acccggaatg
     1381 acctttgccg agagcatggc gtcgccggct ttggctttcg tgcggttgcg ggcgaccagg
     1441 atttgctgca cacgggttgc cgattcaatc aagacctggt tgtttttgct gccgcccgat
     1501 tccagatatc cttccaatag ataggccaga ttcgacaacg acagcgatgg agatcgctgc
     1561 gccaggtttt gaaatgcctc gctgcgttcc ttgctccgca gttctgccgc cgtcaagtgc
     1621 agttcgcccg acagtgggct tttgccctgc aatcccatcg agactgccac ctcgtccagc
     1681 acatcagcca ttgagccgcc ggccagcagg ccagccgaga tacggccaat ggcaatgggc
     1741 agttctttgt caatttcacg cccccgccct ttgaccttat ccgtcagcca ggccatcggc
     1801 gcatagaaca acagaccgcc aatcgccagc gccggcacac cgggcagcac tgtccagacg
     1861 accagtgcgc cgccaagacc gagcaggatg cgcccccatt tgaacgtcgc tggacgccag
     1921 ttcacgcccg atgcggccag tttgtattcc agcgagtcag ggttcatctc gctgagcttg
     1981 cgagtttgtt caagcgagta ggcgtcagac agggcttcgg ttgccttgcc ctttttcatg
     2041 ccgcccattt tggggccgaa cagcagccag atcaatgcgg ctgccaggat gccaatgaga
     2101 tataccgtca tgctttaact ccttccttat tcttccagtt cggccagttt ttcccagcgc
     2161 ggcgcgccgg gttggctggt ttcgtcatac tgccagaggg gatcaaacca gacatcccca
     2221 ccccgcagtt cacgggcgat ttgtgcaatc gtggtcacac accgcttttc atgccggatg
     2281 ccgatctgca ccagcatatc caccgaatcg gccaccatct gattggcatc cactttgcgc
     2341 accccggcat cagcccccat gatggtcgcc agccggctgg ccgcttcacg cggactgtcg
     2401 gcatggaagg tgcaggcccc ggagtggccg gtcatcatgg cccggaacaa cgcctgcgct
     2461 gcccggccat cccgaacctc accaatgatc agatagtccg gcgacatacg catggcatcg
     2521 tccacaccat cggccagggt gtaaggctgg acttcggtgc cgacggcacg ggggcgggct
     2581 tcaatcgttt gcacggtgtc acgggcaatc cagatttctt ctggatcttc aattttcaaa
     2641 acgcgccagg ccggagggag aaagttgcag atggccgaca gcagggtggt ctttcccgtg
     2701 cgggtgccgc cggtgatcag tatccgctgc cccgatgcca tcccccggcg cagggtgtcc
     2761 atcatgtcgg cgctcaacgc ctggcgttca atcagccatt caggaagcac cggcttttgt
     2821 tcgtacaacc ggatgttgat ggagtgattg cgccctgggg gcgtaatcac ccggtgcagg
     2881 gctttcacac gcccaccacc aggattgtcc tcagtgcgcg gcagcttggc gttcacggat
     2941 gggttggttt cgttcagcga tttgttctgc gggccgagca gccggtccag cacacgctca
     3001 acttcttccg cctggggccg catgggaatc gtctcccacc ggacttcacc cttccgcata
     3061 atctggacca acccggagga atacacggtg atctccgaga gatcgttccg ggacggtgga
     3121 agcagcaggt cgagaaagcc caatcccatc agccggcggg ccaattcccc ggccagttca
     3181 acttcaaacc cctgaccggg attggcgctg cgccggcgca gggcttgcgt caccagtgag
     3241 gcaatgcgct ccgcaacccg ctgccgctct atttcgtttg gccgttgcag cagggccggc
     3301 gggaactccc gattgatgac atcaatgact tcggtggaga cttcaattcg caggtcgccg
     3361 ctgacggctt gctgcgcggt ctgtttgtcg tcaaaaacac ttaacaggct catttttcct
     3421 cctcacttgc aataacctga ttccaggttg cgtcctcggc gtaccgcccg tccagccctg
     3481 cggcaacgat ctgacgatag ttgttcatca gactcgcact cgttgttttg tctggaggca
     3541 ggtagcccaa aaagcggccc acacgaacct cctcgccatc cgtgcgctca cacaggtcaa
     3601 ggtacccgag gtcggggtca aggatgaccc cgaccatccg gtataccggc gtttccgcgt
     3661 gcggtgtttc gctgcgcacc agggcatagc acagcagcgg cagcgaccgc acccgggcat
     3721 caacccgggc aagaatcccg gcggattcgg gggcagtgcc ggcctcgtcg ttcacacatt
     3781 caaacacggc tgcatagtcg ggcaggtgga caatctgaat gatggtatcc attacccgcg
     3841 acctccaagc aggcttccca gagagaagcc cttcttctgc gatgagtcgg acgttgtttg
     3901 tccagccacc ccagggaaca ggccgttgat gatggtgcgg atgcctttcg taaagccatc
     3961 caccttccca accaccggga cgcgatcatc ctgtgtgttc gggatgtttt gatcgtatgg
     4021 aatgaccgcc aggaccggcg gcgcccatcc atagctttgc accagttctt ggtaaaactg
     4081 gcttggcgtg attcgcgacc ggtcactggc ctggttcagc accataaaga tcgattcttg
     4141 tgggatgcgg tgttcgttgc gcatccgttc catcagcagc accagcaggt gccgggttgc
     4201 tgccaggtca gccagggtgg gccgggagac aatgatcgca gtgttggccg ccatcaaggg
     4261 gtgcatcgtc cagattcctt cgctgcacgg caggtccagc atgacgccgg cataactgcg
     4321 cgtccagctt gccatcacca gcgagtagat cgagttggcc tcggctttcc cccctgctga
     4381 agatgaagat tcagcagccc gggcatattc caccgagttt tcaggcgcga cgatcacatc
     4441 cagcccttcc ttcgtttgga tcgcactggc aaacccgtcc cccggacgag caaaaaactc
     4501 gccggcattg ggggtgtatt tcaatcccag gttcatcgcc gctgagggcg gcaggcccag
     4561 tgacatcagc agggtgcgga tgttcaggcg cacggccagt tcataggcga tattctcggc
     4621 cagggttgaa cgaccggcac cgcctgagcc agacagaaac gccacaacgc gggttccggt
     4681 caccgatgtc gccgcccgaa accccattgc gctttgcaga ggagcctgtg ccgcaccgcg
     4741 cgcccgttca gtggacacgg cagcatagcc gccctggacc acttcgcccc agttcaccgg
     4801 gaggacaaac acgccgcgca ccacactgac cttctcaaac gcaccccgaa actcacggtg
     4861 cgctgcttgc aagacaacca gcgccacacc attccagacc tgcatccggg ccagggctgc
     4921 aatcagcgta tcaggattgg gcgcaatgtc accctgcacg accagcagat tcggcttcaa
     4981 ctgttccaga ttcttctcga aatcagacca gccggtgaca accgacgata cactgaaacg
     5041 cgtatctgat tgaaatgccg gcatcatctg gtagaacacg gtctctgcac ctgtgccggc
     5101 cagcatggtc aaaacttttg cttcctgatt caacatggca gcatttcctt caattcaatt
     5161 agggcttggg cgccagggtc ggcagcaggg tgggttcagg ggtggcggtg gcgacaggtt
     5221 cgggcgttgc agtcggatgg tagtaggtcg tcggggtggc agccgccgat acctggttgt
     5281 tgccgttgat ggacttgtac aggctcgcca ggttaagcgt cagaatatct tcttcggcaa
     5341 ccaccagccc gctgctcggt tcaagcgcca gatacaccac gccaaactga tccagcgcag
     5401 caatcagggt cgccggattc acgaacacgc ccggcgcgat ttccattggg gtcgtcggca
     5461 catccagcac aatcacgccg ctgtccttgc tggtcgcggc tgaactggca aacagttcct
     5521 cctggctcgc gccggcggga atttcctggt actggaagga ctgcgggacc atcagaacac
     5581 gcagcccgct gatcgagatc cggcctaccg gccccatcag ggggatgggc gttgcggtcg
     5641 gggtgggcgt cagcaggctg ttcgggtctg cggataggtc gggaacaaga actgaggctg
     5701 cggggatgac actggacagt cccgactggc tgccgaccac atccggggtc aacaggccaa
     5761 tgatcgtcac cgtctgaccg ggacgcagca atccagcgac gccgctggac aggtccacgt
     5821 tgacggcaat cgccacatgc cccggttcaa gctgcgccgg gatgccagcc tgggcattgt
     5881 cgcccagggt ggaggcggta atggcatcac ccgccgcgcg ctgcac
//
LOCUS       DAOQWR010000145         5855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13928_length_5855_cov_5.565345, whole genome shotgun sequence.
ACCESSION   DAOQWR010000145 DAOQWR010000000
VERSION     DAOQWR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13928_length_5855_cov_5.565345"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PKK36_10110"
     CDS             <1..892
                     /locus_tag="PKK36_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR94944.1"
                     /translation="AKVLETCGIAAVMRELKLELADFKNTATFECAENRVARKLDLAK
                     AAADADVIITIPKPKTHMQMTYTGAVKNQFGLIPGTRKAQYHYRLETREWLAMLMVDI
                     NRMARPALCIMDAVYGMDGEGPSSGNPRKIAAVLASSDLPAMDAVCCAIIGQDPEQVP
                     TQAACRESGFGTTELEDIEVLGDDWKKFRLRDFELVKRVSSVPFLPLPKAAHKWIREQ
                     LSPRPRIINDKCILCMACFKGCPVEPSAIDPRREAARQVNDKTCIRCYCCHEFCPEKA
                     IRLRKSWLDRIFRLSALIGR"
     gene            complement(1454..2113)
                     /locus_tag="PKK36_10115"
     CDS             complement(1454..2113)
                     /locus_tag="PKK36_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR94945.1"
                     /translation="MTGAPKKRILVVDDHSIVRHGIATLLNSEPDLEVCGEAGSFEQA
                     LELLAQTAPDVMLVDITLKDRNGLDLIREIRAAGHTLKVLVLSIHDESVYAERALQAG
                     AQGYVMKEKGDDDIVRAIREVAAGKTYVSPEISEQILRQKIGGMPSDTDAGGIGSLTD
                     RELEIFECIGRGLDTKAIAEKYALSARTVEVHRAHIKKKLGCQSAAEVVREAVRWVER
                     G"
     gene            complement(2110..3828)
                     /locus_tag="PKK36_10120"
     CDS             complement(2110..3828)
                     /locus_tag="PKK36_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNR94946.1"
                     /translation="MSTTPDRKKQTAAPLWIWAPGIAVLVLGLLLTFAAWRAAKNREL
                     ENINAGFQIEADAYAGDIARALNLFQSVLLSLRDLHSISENISAENFAEFVEKGMAHQ
                     QMVLGAFGFAQYIPHEMRPAYEGEGREAVITERTGTNLVPAAARPAYFPLTYQSPAGA
                     LGVPTGFDFGSTPRNMAAIMQMFSAGQPAVGGHDITEESGKPKGRYVFAPIMQATRDE
                     KTQAVAIAPVGFAVAVLNVNRLLDSLEPSPESKGMHAAVVDSFTGGARPEPLKYRALL
                     RVANEDWMFICTASSAWLRAHQTGQPATVLASGLIITALLTGLLLLLAGRACHVEQLV
                     RRKTADLEEAGKLLREEMDERLRLEDEILEAGSRERQRLGRDLHDSLGQKLTGAQYLS
                     SALSKHPADAPGEGLRDSAAQINTILRDCVAQVRRIAKGLAPVELDDEGLSAALQRLC
                     SETAATFGVECGFSCEPQKIIIPSPAVTHLYHITQEAINNAIRHGKAGRIDVRLTLAG
                     DDGELTVRDNGQGISRAEPRETGMGLRIMRHRATVIGGSCIARNREEGGVQVTCRFAR
                     ERGGDQ"
     gene            3948..5189
                     /locus_tag="PKK36_10125"
     CDS             3948..5189
                     /locus_tag="PKK36_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94947.1"
                     /translation="MKKYLAVMALLAGSCPGQNFSRSMGEFTPWGGTATSASYAVRAV
                     SGDFTGGVSTGANFRNDASYAGQFCTLQVVELSAGSTNILERTTEQIRALGLYDDGSI
                     APIENDCSWGVLSGPVADIGPAGLLLAASTGVDAAAEIAAVYDGVTGVLGLMILHDSA
                     AVSNQTQHLTGTWGVWTYNPATGRFIGQFTFCNRADSGRMMTEPFRFVIPEGEDFRLY
                     RPDGTTTDGDHYKDVTAQVLAQLADGELNTGDCVTVTGIEVVRLAGKLPPPELPWQVW
                     SEGRPAGWLADSDGDGIPDEYENQYAPPLDPLNPDDALLDPDGDGMSSLAEWIAGTDP
                     TDAASLLEMAAIEPDEARRPVVTWLSASNRTYTIYWSTNRLDNFVPLQEDIAGTPQWN
                     SFTNAVPSGAPAVFFRIGVRR"
     gene            5251..>5855
                     /locus_tag="PKK36_10130"
     CDS             5251..>5855
                     /locus_tag="PKK36_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR94948.1"
                     /translation="MNPEDQFQLSFEDVKPAPAKPAAAAVEPEAESAPEEERPASVCP
                     DDRLFQRLPLRRRRAREILRLTGDTRSRTVLIIGGDAMTQEVLRSQPGIWETASWLEQ
                     PELLFDEAETQPPAGIPYEDAQFDIVVAIDVLQYLSKPDVFIRDCHRVLKSTGLLILQ
                     MPHLKSWSLLPLLKRLLDLRDDEGRIGQVRRGYRAREVFDLL"
BASE COUNT         1050 a         1737 c         1967 g         1101 t
ORIGIN      
        1 ggcgaaggtg ctggaaacct gcggcatcgc agcggtcatg cgcgagctga agctcgagct
       61 cgccgacttc aaaaacaccg cgacctttga atgcgcggag aaccgcgtgg cgcggaaact
      121 ggatctcgcg aaggcggcgg ccgacgccga tgtgatcatc acgatcccca agcccaagac
      181 gcacatgcag atgacctata ccggggcggt gaagaaccag ttcggcctga tccccggcac
      241 gcgcaaggcg cagtatcact atcgcctcga aacccgcgag tggctggcga tgctgatggt
      301 ggacatcaac cgcatggcga ggcccgcgct ctgcatcatg gatgcggtgt acgggatgga
      361 cggcgagggg cccagcagcg gcaatccgcg caaaatcgcc gcggtgctgg cgagcagcga
      421 cctcccggcg atggatgcgg tctgctgcgc gattattggt caggatccgg agcaggtgcc
      481 gacgcaggcc gcctgccgcg agtcgggctt cggcacaacc gaactggaag acatcgaagt
      541 gctcggggat gactggaaaa aattccgcct gcgcgatttc gagctggtga agcgggtcag
      601 cagcgttccg tttctgccgc ttccgaaggc cgcgcacaaa tggatacggg agcagctcag
      661 tccgcgtccg cggatcatta atgataaatg catcctgtgc atggcgtgct tcaaggggtg
      721 cccggtggag ccgtccgcga ttgatccgcg aagagaggcg gcccgtcagg tgaacgacaa
      781 aacctgcatc cggtgctact gctgccacga attctgcccc gagaaggcga tccgcctgcg
      841 gaaatcatgg ctggaccgga tattccggct gagcgcgctc atcgggagat gatcgtcggc
      901 cttgcggcgg gcgtgaaccg cccgtccgca atccttctga aaaaatgcaa tccgtgaagt
      961 tcgcttaatc cccatacaga tatatcggta tccgatatat cggaaccgat atattaatcc
     1021 ggctgcataa aaggcagcat ctggggcggg ggggggcggt cgcttcggcg gccgtcgcgg
     1081 cgctttggag atgagagcga tccgttcccc gtacatggcc atgcctcatg aaaaggcgaa
     1141 gagatgactg ggtcttgagc cctgagaaac atgcgggaac ggtttgcttg aaaacgggac
     1201 tggacaaaac tttcagagat gcggcgggcg tgaaccgccc gtccgcaatc cttctgaaaa
     1261 aatgcaatcc gtgaagttca ctcaatcccc agacagatat atcggtatcc gatatatcgg
     1321 ataccgatat attaatccgg ccgcataaaa ggcagcatct ggggcggggg gggcggttgc
     1381 tccggcggcc gtcgcggcgc gactcggcaa ataccacttt cacacgattt cgcccgcgca
     1441 ttttaatttc tgattatccc cgctcgaccc agcggacggc ttcgcggacg acttcggccg
     1501 cgctctggca tccgagtttc tttttgatgt gagcgcggtg gacttcgacg gtgcgcgcgc
     1561 tgagcgcgta tttttcggca atggccttgg tgtcgagtcc gcggccgatg cactcgaaga
     1621 tttccagttc gcggtcggtc aggctgccga ttccgcccgc atcggtgtcg gacggcatgc
     1681 cgccgatttt ctggcgcagg atctgttccg agatttcggg gctgacgtag gttttcccgg
     1741 cggcgacttc acggatggcg cggacgatgt cgtcatcgcc cttttccttc atcacatatc
     1801 cctgcgcgcc ggcctgcagg gcgcgctcgg catagacgga ttcgtcgtgg atggaaagca
     1861 caagcacctt gagcgtgtgg cccgcggccc ggatttcacg gatgagatca agcccgttgc
     1921 ggtctttgag cgtgatgtcc acaagcatga catccggcgc cgtctgcgcg agcagctcaa
     1981 gcgcctgctc gaaggatcca gcctcgccgc acacctcgag atcgggttcg ctgttcagca
     2041 gggtcgcgat gccgtgacgg acaatggagt gatcgtccac gaccagaatt ctttttttcg
     2101 gcgcgccggt cattgatccc ctccgcgttc gcgggcaaaa cggcaggtga cctgcacgcc
     2161 gccctcttcg cggttgcggg cgatgcagct gccgccgatg acggtggcgc ggtggcgcat
     2221 gatgcgcagg cccatgccgg tttcacgcgg ctcagcgcgg gaaattccct gcccgttgtc
     2281 gcggacggtc agttcgccgt cgtcgccggc cagggtcagg cgcacgtcaa tccgaccggc
     2341 ctttccgtgg cggatggcgt tgttgatggc ctcctgcgta atgtgataga ggtgcgtgac
     2401 ggccggggag gggatgatga tcttctgcgg ctcgcaggaa aagccgcatt ccacgccgaa
     2461 cgtggcggcg gtttcggagc agagccgctg gagggcggcg gaaagccctt cgtcgtccag
     2521 ttccaccggc gcgaggccct tggcgatgcg ccggacctgc gcgacgcagt ccctgaggat
     2581 cgtgttgatc tgcgcggccg agtcgcgaag gccttccccg ggggcatccg cgggatgctt
     2641 tgaaagcgcg ctgctgagat actgcgcgcc ggtgagtttc tggccgaggg agtcatgcag
     2701 gtcgcgtcca agccgctgcc gttcgcggct tccggcttca aggatttcat cttcgagacg
     2761 aaggcgctcg tccatttcct cgcgcaggag tttgccggcc tcctccaggt cggcggtctt
     2821 tctgcggacg agctgctcca catggcaggc tcggccggcg agcagcagca gcaggcccgt
     2881 caggagcgcg gtgatgatca ggccggaggc cagcacggtg gcgggctgtc cggtctggtg
     2941 cgcgcggagc catgcgctgg atgccgtgca gatgaacatc cagtcttcgt tggcgacgcg
     3001 cagcagggcg cggtatttca gcggctcggg ccgcgccccg ccggtgaatg aatccacgac
     3061 ggccgcgtgc atgcccttgc tttcgggcga aggctcgagc gaatcgagca ggcggttcac
     3121 gttcagcacg gccacggcaa atccgacagg ggcgatggcg acggcctgtg ttttttcatc
     3181 gcgggtggcc tgcatgatcg gcgcgaagac atagcggccc ttcggtttcc cgctttcttc
     3241 agtgatgtcg tgccctccga cggcgggctg tccggcggaa aacatctgca tgatggcggc
     3301 catgttgcgg ggggtggagc cgaaatcgaa accggtggga acacccagcg cgccggcggg
     3361 gctctggtag gtcaggggga aataggccgg ccgcgcggcg gccggaacaa ggtttgtgcc
     3421 ggttcgttcc gtgatgacgg cttcgcggcc ctcgccctcg taggccggcc gcatttcatg
     3481 agggatgtac tgcgcaaagc cgaacgcgcc gagcaccatc tgctggtggg ccatgccctt
     3541 ttcaacaaac tcggcgaagt tttcggcgct gatgttttcg gagatgctgt gcagatcgcg
     3601 cagcgaaagc agcacggact gaaacaggtt gagcgcgcgg gcgatgtcgc cggcataggc
     3661 atcggcttcg atctggaatc cggcattgat gttttccagt tcacggttct ttgccgcccg
     3721 ccatgcggcg aaagtgagaa gcagtcccag cacgagcacg gcaatgcccg gcgcccatat
     3781 ccagaggggc gcggccgtct gctttttgcg atctggcgtt gtgctcatat tgaggttgag
     3841 cctgtgcatt tccgtgtcgc gaggcaagca gaaagaaacg cggcaaaccc ccggccactt
     3901 gaaacttgat ccggtcacgg tctgcggagt atcctgtccg gcgtgacatg aaaaaatatc
     3961 tggcagtcat ggcgttgctg gcggggtcgt gcccggggca gaacttcagc cgctccatgg
     4021 gcgaattcac gccgtgggga ggaaccgcga cttcggcgag ttatgccgtg cgggcggtct
     4081 cgggcgactt cacgggcggg gtgtccaccg gcgcaaactt caggaacgat gcgtcctatg
     4141 ccggccagtt ctgcacactc caggtggttg agctttcggc cggttccacg aacatccttg
     4201 aacgcaccac ggagcagata cgggcgctgg ggctttatga cgacggatcc atcgcgccga
     4261 tcgagaacga ctgttcgtgg ggtgttctga gcggtccggt ggcggatatc ggacctgccg
     4321 gcctgcttct ggcggcctcc acgggcgttg acgccgccgc ggagatcgcc gcggtgtatg
     4381 acggcgtgac cggagtgctc gggctgatga tcctgcacga ctccgcggcc gtttccaacc
     4441 agactcagca tctcaccgga acatggggcg tctggaccta caatccggcc acgggccggt
     4501 tcatcgggca gttcaccttc tgcaaccggg cggacagcgg ccggatgatg accgaaccgt
     4561 ttcgttttgt gattcccgaa ggcgaagatt tccggttgta ccggccggac gggacaacga
     4621 ccgacggcga ccattacaag gatgtcaccg cgcaggtgct ggcgcagctg gcagacgggg
     4681 agcttaacac cggcgactgt gtgacggtga cgggaattga ggtggtgcgc ctcgcgggca
     4741 agctgccgcc gcctgaactg ccctggcagg tctggagcga gggccgcccc gcgggctggc
     4801 tggcggacag cgacggcgac ggaattcccg acgaatacga gaatcagtat gcgccgcccc
     4861 tggatccgct gaatccggat gatgcgctgc ttgatcccga cggcgacggc atgagcagcc
     4921 ttgccgaatg gattgccggg acggatccga cggatgccgc ctcgctgctg gaaatggcgg
     4981 cgatcgaacc ggatgaggcg cggcggccgg tggtcacgtg gctgagcgca agcaaccgga
     5041 cctacacgat ctactggtcc acgaaccggc tggacaactt tgttccgctg caggaggaca
     5101 tcgccggcac cccgcaatgg aactccttca caaacgccgt cccgtccggc gcgccggcgg
     5161 ttttcttccg cattggggtg cggcgctgat cttcgtgctt gttatgcggt cgtccctgag
     5221 ggataatgcc gcgcaatttg agaagacgcc atgaatccag aagatcagtt ccagttgagt
     5281 tttgaggatg tgaaacccgc gccggcgaag cccgcggccg cggccgtcga gccggaggcg
     5341 gaaagcgccc ccgaggagga acgtccggca tccgtctgcc cggatgaccg gctgttccag
     5401 aggctgccgc tgcgccggcg gcgcgcgcga gagattctga ggctgacggg cgacacgcgc
     5461 agccggacgg tcctgatcat cggcggcgat gccatgacgc aggaagtgct tcgcagccag
     5521 ccgggcatct gggaaaccgc ttcatggctt gagcagccgg aactgctgtt tgacgaggcg
     5581 gaaacccagc cgccggccgg catcccctat gaggacgcgc agtttgacat tgtcgtggcc
     5641 attgacgttc tccagtacct gtcgaaaccg gatgtcttca ttcgcgactg ccaccgggtg
     5701 ctgaagtcca cgggactcct gattctgcag atgccgcacc tgaaaagctg gtcgctgctg
     5761 ccgctgctga agcggctgct ggacctgcgc gacgacgagg ggcggatcgg acaggttcgc
     5821 cggggctatc gcgcgcggga ggtgtttgac ctgct
//
LOCUS       DAOQWR010000146         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_13970_length_5844_cov_4.464329, whole genome shotgun sequence.
ACCESSION   DAOQWR010000146 DAOQWR010000000
VERSION     DAOQWR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13970_length_5844_cov_4.464329"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKK36_10135"
     CDS             complement(<1..271)
                     /locus_tag="PKK36_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR94949.1"
                     /translation="MSGHSKWSTIKHKKAASDAKRGKVFSKIAREIMVAARSGGGDPN
                     ANVTLRALLQKARSANMPSDNIERAIKKGTGDLGGAALEELTYEGY"
     gene            complement(286..2316)
                     /gene="fusA"
                     /locus_tag="PKK36_10140"
     CDS             complement(286..2316)
                     /gene="fusA"
                     /locus_tag="PKK36_10140"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR94950.1"
                     /translation="MKNIAIADMRNFVLLGHTGSGKTTLVDALLYKMGINDRLGSVDD
                     GSSMADWTEEEKTRKISTSAKPFDGSYKDAAGRITRMVFMDTPGYADFYGQVVAATSV
                     ADAALVAVDVNSGIQVGTARVWRRCEDLQLPRGVVITGMDKENANFDRALAAIQEVWG
                     ARCVPVVIPSGGAQVVDVLASKSVPAEIADQVSAIKGSLVEAAAETDDALIEKYLGGA
                     ELTPDEISAGLRGAVASGKLIPVFVTDAKKGVGLVELLDGIRRLFPDPSVRPAKDIED
                     KVVDASAGAPAIGLVWRSVNDPFVGQLTYVRVYGGTISADRELFNVTRGEKERVGALY
                     LSRGKKQETVPEVQAGDIVAIAKLKHTALNDTLGAPGTTRKLPVIVFPHPVMSYAVVA
                     KTQGDEDKLGSGLHRICEDDPTLKVERTSDTHELILSGLGDVHLDAAVKRLKERSNVD
                     VLLQTPKVSYKETVTGRGEGHYKHKKQSGGRGQYGEVYLRIERRDPSEEEWFVNAIVG
                     GAIPGNFIPAIEKGLVEGMNKGAVAGYPVTNVKVTVYDGSFHDVDSSEIAFKIAAQRA
                     FREGMANAKPVLLEPIMKLKVMVPDQYMGDVTGDLNHKRGRILGMGSEDGMQVITAEV
                     PQAEFFQYCPQLRSMTGGRGSFEMEFSRYDVVPANIAQKVIAAAEKLKEDEE"
     gene            complement(2483..3556)
                     /locus_tag="PKK36_10145"
     CDS             complement(2483..3556)
                     /locus_tag="PKK36_10145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNR94951.1"
                     /translation="MKKSLSLCAAAVFGLSVFTTSAALPVIARDPYIGAIVIDAATGE
                     TLFEDNADRTGYPASMLKLMDMLIILERVQSGLVGYDARVTTSAAASRMGGSQVYLKE
                     HEVFSLEDMLYALMIQSANDAALALAEHFAGAKDGFVHLMNQKAQELGMDSTRFFSPH
                     GLPPGEGQSPDVTTARDFAKLCRELCRHPDIFRFTSARERDFRDGTFIMRNHNNLLGV
                     FPGCDGFKTGYFRAGGYSIAATAEREGRRVIAVVLGSVSKQVRDAKARELLSIGLANA
                     LSTPQPVIPPAAPVAASQLPAVPEEADDLELENEPQDLPPAGGGCGGFWTGLLAGLGV
                     GALAYAGVKLKQRRDSRNFIRRS"
     gene            complement(3553..3840)
                     /locus_tag="PKK36_10150"
     CDS             complement(3553..3840)
                     /locus_tag="PKK36_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNR94952.1"
                     /translation="MARKKAVIQNEQGIHCRPAAVILKEVASYPGKVLVECDNGESPL
                     DSVLDLMALCLSRGTTVFINVEGPDDEAYCDKLVKLFEYHFDFPPRGQGDA"
     gene            complement(3846..4958)
                     /locus_tag="PKK36_10155"
     CDS             complement(3846..4958)
                     /locus_tag="PKK36_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94953.1"
                     /translation="MTILLNKTLRLFLDSIGRRDEYEFYLNKFQSGQSQAFAILCPDR
                     AGFEEVASVFTFDLDFLLRLELLPVILLAGEDGMALKRILEESQPCYNFCEISHREED
                     ITKLLEVSRRAGRIPVLCYPKETRTDALLSLVPFISSRVHLIRLQGILRDTAGREIFY
                     YYTQKENPVEIAPEDAELVRLAATLLEARPGVHISVASPWRLLQELFTVKGAGCVFRR
                     GSRILRFGSISECDTVRLGALMENSFGKKLASPATFAGRVTGIYVEENYTGAALLETH
                     PAGRYLSKFAVGTEARGEGLANELWQDVTRDQPALFWRAREDNPINHWYEKQADGFQR
                     SGRWRVFWRGIAPEQIPVVIRYSLEKPDDFVQPGGA"
     gene            5089..5268
                     /locus_tag="PKK36_10160"
     CDS             5089..5268
                     /locus_tag="PKK36_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94954.1"
                     /translation="MKDHKLVCAGDHKGHICLLASQNKFDQIMEITKKPAFICFNCGR
                     VADNACNLCNPMPLK"
     gene            5406..>5844
                     /gene="nadA"
                     /locus_tag="PKK36_10165"
     CDS             5406..>5844
                     /gene="nadA"
                     /locus_tag="PKK36_10165"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNR94955.1"
                     /translation="MSAGRMYEILKAKLARVVPDIELRYKAEIAEEIVELKKKRNAVI
                     LAHNYMEPALFYSIADFTGDSLDLSRKAATTDKDIIVFCGVRFMAETAKILSPDKTVL
                     LPAEKAGCSLAESITADDVREIRRQFPDAPVVTYVNTYADVKAE"
BASE COUNT         1204 a         1759 c         1653 g         1228 t
ORIGIN      
        1 cataaccctc ataagtcagc tcttcaagag ccgccccgcc gaggtccccg gttcccttct
       61 tgatggcccg ctcgatattg tcgctgggca tgttggccga acgggccttc tgcagcaaag
      121 cgcggagcgt gacattggca ttcgggtcgc cgccgccgga ccgggccgcc accatgattt
      181 cgcgggcgat cttgctgaac accttgcccc gcttggcatc ggaggccgcc ttcttatgct
      241 tgatggttga ccatttgctg tgaccgctca tacatcaaac cccttttatt cttcatcttc
      301 cttgagcttc tccgccgccg caatgacttt ctgggcaatg ttggcgggaa ccacatcata
      361 gcgcgagaac tccatttcaa acgatccgcg gccgccggtc atgctgcgaa gctgcgggca
      421 gtactggaaa aattcggcct gcggcacctc cgcggtgatc acctgcatgc cgtcttcaga
      481 acccatgccg agaatgcgtc cgcgcttgtg gttcagatcg ccggtgacgt cgcccatata
      541 ctggtcgggc accatgacct tcagcttcat gatgggctcc agcagaacag gctttgcatt
      601 ggccatgcct tcgcggaacg cgcgctgggc tgcgattttg aaggcgattt cggaggaatc
      661 cacatcatgg aaggagccgt catagaccgt aaccttcaca ttggtcacgg gatagccggc
      721 cactgcgccc ttgttcatgc cctcgacgag gcctttctcg atggcgggaa tgaagttgcc
      781 ggggatggct ccaccgacga tggcattcac aaaccattct tcttccgagg ggtcgcggcg
      841 ctcgatgcgc agatacacct cgccatactg cccgcggccg ccggactgct tcttgtgctt
      901 gtagtgcccc tcgccgcgcc ccgtcaccgt ttccttgtag gaaactttcg gggtctgcag
      961 caggacatcc acgttgctgc gctctttcag gcgtttcacg gcggcatcca gatgcacgtc
     1021 gccgaggccg gaaaggatca gctcatgcgt gtccgaagtc cgttcgacct tgagcgtcgg
     1081 gtcatcttca cagatgcgat gaagccccga gcccagcttg tcttcatcgc cctgtgtctt
     1141 ggcgaccacg gcgtaggaca tgacgggatg cgggaataca atgacgggca gcttgcgggt
     1201 ggttcccggc gcgccaaggg tgtcgttcag ggccgtgtgc ttcagcttgg caatggccac
     1261 gatgtcgccg gcctgcactt cgggcacggt ttcctgcttc ttcccgcgtg aaagatagag
     1321 ggcgcccacg cgctcctttt caccgcgagt cacattgaac agctcgcgat cggcgctgat
     1381 ggtgccgcca tagacgcgca cgtaggtgag ctgtcccacg aagggatcgt tgaccgagcg
     1441 ccacaccagg cctatcgcag gagcccccgc tgaagcatcc accactttgt cttcaatgtc
     1501 tttcgccgga cggacggagg gatccggaaa caggcgcctg atgccgtcca gcagctcaac
     1561 gagtcccacg ccttttttgg catctgtcac aaagacggga atcagtttgc cggaagccac
     1621 ggctccgcgc agaccggctg atatctcatc cggcgtgagt tcggcgccgc caagatactt
     1681 ctcaatcagc gcgtcatcgg tttccgcggc ggcctccacc agactgccct tgatggcgga
     1741 gacctgatcg gcgatttccg ccggcacaga tttggatgcc agcacatcca cgacctgcgc
     1801 gccgccagag ggaatcacaa ccgggacaca gcgggcgccc cagacttcct gaatggcggc
     1861 cagggccctg tcaaaattcg cgttttcctt gtccattcca gtgatgacga ctccgcgcgg
     1921 gagctgcagg tcctcgcagc ggcgccacac gcgggcggtt ccgacctgaa tgccgctgtt
     1981 gacatccacg gccaccagag cggcgtctgc gaccgatgtg gccgcgacga cctggccgta
     2041 gaaatcggca tagcccgggg tgtccatgaa caccatgcgc gtaatccgtc ctgccgcatc
     2101 cttgtaagat ccgtcaaaag gcttggctga agtcgaaatc ttgcgggtct tctcctcttc
     2161 agtccagtcc gccatgctgg acccgtcatc aacggaaccc aggcggtcat tgattcccat
     2221 cttgtagagc agagcatcca ccagggtcgt tttgccgctt ccggtatgcc ccagcagcac
     2281 aaaatttctc atatcggcaa tggctatgtt cttcatactc tcatcctcgc attcattaca
     2341 gggataatcc cggttcacaa taaacaagtc tgatatagat acacaaagct tccctgcggg
     2401 gcaaccgttt tcggcaattt tcagggtttc agagtgtctg cgcttctccg ggcggaaagg
     2461 ttttcctcac tgcgcatttg cgttatgagc gccgaatgaa gttgcggctg tcacggcgct
     2521 gcttgagctt gacgccggca tacgccagcg ccccgactcc aagccccgcc agcaggcctg
     2581 tccagaatcc gccgcatccg ccgcctgcgg gcggaagatc ctgcggctcg ttttccagct
     2641 ccaggtcatc cgcttcttcc ggcacagcag gaagctggct tgccgcgacg ggcgcggcag
     2701 gcgggatcac gggctgcggc gtggaaaggg cattcgcgag accaatggag agcagttcgc
     2761 gggcctttgc gtcgcgaacc tgttttgaga cactgcccag caccaccgca atcacccgcc
     2821 ggccttcacg ttcggcggtg gcggcaatgg aatagccccc ggcgcggaaa tagccggtct
     2881 tgaaaccgtc gcagccgggg aaaacgccca gcaggttgtt gtggttgcgc ataatgaagg
     2941 tgccgtcacg gaagtccctt tcacgcgcag acgtaaaacg gaagatatcg ggatgcctgc
     3001 atagctcccg gcagagtttg gcaaaatcgc gcgcggtggt gacatccgga ctctggcctt
     3061 cccccggcgg caggccgtga ggcgagaaga aacgcgtcga atccatgccg agttcctgcg
     3121 ctttctgatt cattaaatga acaaaaccgt ccttggcgcc cgcgaaatgc tcggccagcg
     3181 ccagcgcggc gtcattggcc gactggatca tcagggcata gagcatgtct tccagagaga
     3241 agacttcatg ctccttcaga tacacctgcg aaccgcccat ccgcgatgcc gccgccgaag
     3301 tcgtcacccg ggcatcatag ccgaccaaac ccgattgcac gcgctcaaga atgatgagca
     3361 tgtccatcag cttgagcatg ctggcgggat atcccgtgcg gtccgcattg tcctcaaaga
     3421 gagtttcgcc ggtggcggca tcaatcacaa tggcgccgat atacggatca cgggcgatca
     3481 cgggaagcgc ggcagatgtg gtgaacacgc tcagcccgaa aacggccgcc gcgcacaggg
     3541 acagtgattt cttcatgcat ctccttgccc gcgaggggga aaatcgaagt gatattcaaa
     3601 cagtttgacc agtttgtcgc aatacgcttc atcatcgggg ccctccacat tgatgaagac
     3661 ggtggttcct ctggacagac acagcgccat caggtcgaga acagagtcca gggggctttc
     3721 gccgttgtcg cattccacaa gcactttgcc cggatatgac gccacctcct tgagaatcac
     3781 ggcggcgggc cggcagtgaa tcccctgctc attctgaatg accgccttct ttctcgccat
     3841 gatacttagg ctcccccggg ctgcacgaag tcatccggct tttccaggct gtagcggatc
     3901 accaccggga tctgctccgg cgcgattccg cgccagaaga cgcgccagcg cccggaccgt
     3961 tgaaaaccgt cggcctgctt ttcgtaccag tggttgatgg gattgtcctc ccgcgcgcgc
     4021 cagaacaggg cgggctggtc ccgggtgacg tcctgccaca gttcattggc cagcccctcc
     4081 ccgcgcgctt ccgtgcccac ggcaaatttg gaaaggtagc ggcccgcggg atgggtttcc
     4141 agcagcgcgg ccccggtgta attttcctca acgtagatcc cggtgacccg tccggcgaaa
     4201 gtggccggac tggccagttt tttgccaaag ctgttttcca tcagggcgcc aagccggacg
     4261 gtgtcgcatt cgctgatact cccgaagcgc aggatgcggc ttccccggcg aaaaacacag
     4321 cccgcgccct tgacggtgaa aagctcctgc agaagccgcc agggggatgc cacggagata
     4381 tgcactccgg ggcgggcctc cagcagtgtg gccgcgaggc gaaccagttc cgcatcctcc
     4441 ggggcaattt cgacgggatt ctccttctgg gtgtaatagt agaatatctc ccgccccgcg
     4501 gtgtcccgca gaatgccctg aaggcgtatc aaatgaacgc gcgaagagat aaacggcaca
     4561 aggctcagca gcgcgtcggt gcgggtttct tttggatagc acagaaccgg aatccggccg
     4621 gcgcgacggc tgacctccag cagtttggtg atgtcctctt cacgatggct gatttcgcag
     4681 aaattgtagc acggctggct ctcctcgagg atccgcttga gcgccatccc gtcctcgccg
     4741 gccagcagga taaccgggag aagttccagt cgcaggagga agtccaggtc aaaggtgaaa
     4801 acggacgcca cctcttcaaa ccccgcgcgg tccgggcaga gtatggcaaa cgcctgcgac
     4861 tgccctgact ggaacttgtt gagatagaat tcgtattcat ccctccggcc gatgctgtcc
     4921 agaaacaaac gcagtgtttt gttgagtaaa atagtcatca atagtcctgt tccggcagaa
     4981 gatttacact ccgcagcaaa aaagtcaatt tgccgtaaac ggaatcttgc ccggcaggca
     5041 tcaagactgt agattgaggg gtgaaatcag gaaatcatgg agatacatat gaaggatcat
     5101 aaactggtat gcgcggggga tcacaagggg catatctgtc tgctggccag ccagaacaaa
     5161 tttgaccaga tcatggaaat cacaaagaaa cccgccttca tctgcttcaa ctgcggccgg
     5221 gtggcggaca atgcctgcaa tctgtgcaat ccgatgccct tgaagtaatc tgcataaaaa
     5281 aaagctgttt ttttggtcat ttttaagcgt ttttgttgat tttacggcat tttttgcctt
     5341 tttaggccat tttttcgcgt ttttaggcca tttttggaac tttttagcaa agaaaggctg
     5401 ttccaatgtc tgcaggcaga atgtatgaaa tactgaaggc taaactcgcg cgcgtggtgc
     5461 cggatatcga actgcgctac aaagccgaaa tcgccgaaga aattgtcgaa ctcaaaaaga
     5521 agcgcaatgc cgtcatcctt gcgcacaact acatggagcc ggctctgttc tacagcatcg
     5581 ccgatttcac aggcgattcg cttgatctga gccgcaaggc cgccaccacg gacaaggata
     5641 tcattgtttt ctgcggagtc cgcttcatgg cagagaccgc caagattctg agcccggaca
     5701 agaccgttct tcttccggct gaaaaagcgg gctgctccct ggctgaaagc attaccgccg
     5761 atgatgtgcg ggaaatacgc cgccagtttc ccgacgcgcc ggtggtgacg tatgtaaaca
     5821 cctatgccga tgtgaaggcg gaaa
//
LOCUS       DAOQWR010000147         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_14291_length_5745_cov_5.036555, whole genome shotgun sequence.
ACCESSION   DAOQWR010000147 DAOQWR010000000
VERSION     DAOQWR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14291_length_5745_cov_5.036555"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1147)
                     /locus_tag="PKK36_10170"
     CDS             complement(176..1147)
                     /locus_tag="PKK36_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNR94956.1"
                     /translation="MNYADRARDVLNIEIAGIEKARDNLNGTFDKAVGVILRSLNGGG
                     KIVIAGIGKNLPVGQKIAATLTSTGAPAVFLHPLEAMHGDLGILCAKDVLIALSYSGE
                     THELIELMPLARRFGVKVIALTGEENSTLAKHSDAIISVKIDCEACPFNMAPPASTTA
                     TLAVGDALAMVLLEARGFKKEDYALLHPGGAIGKTLLLKASDIMRTGNRVASVPMGTL
                     VKEAVLAMTGARAGSCVVVDTDRRVKGIITDGDLRRHIGGSLNIASEPVESIMSPDPV
                     TLTEDHLAIDVLALYEKHNIDDLIIVDSNGRLAGLIDIQDLPKLKIL"
     gene            1678..>5745
                     /locus_tag="PKK36_10175"
     CDS             1678..>5745
                     /locus_tag="PKK36_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94957.1"
                     /translation="MQISLNAIDAYRGYIGDEMRSAISIVVGTDGTVIYYDHWEDGYE
                     EDITNPTQLTSQVWGDATAGNGYPPGFPGDLLDAGDVVLLESTIDVTRNSVLIEYDGR
                     DKVATTFPLAMTRAMYAVNPGEVLAESTGIYDIGTHDTLYRAPVGVGTGTGFGTNELF
                     NYTAFYIMADYDYTRIDLDKDNDGIFEETVYLDQGEPYFINGGIVAGATVQGSQPFQC
                     HLVTGDVGSTYEMRWYELWPESQWGTDYFTPVGTRTNQTGGIVPSLVYLFNPNAETIT
                     VHFVTQTSSGSFTVNPNSVYDVFEMPLNSGARFYTTNGAPFLGVNVFDTTVGGGTLYG
                     YVQHCQTHDWGIGLMPANMMSTMGVVGWGPGYGTTGTGDNGNPIWISTITGTTIYVDY
                     DSDPDTGPLVDPQGNRYDVSTNVTALELVQLIDPTDDDQTGMRYYTVDGTVLMAAWGQ
                     DPSRAGPGNPYLDMGYVIPAFPTVLSKKFASLLNDVNGNGYADSGDTIEYFIDVVNVG
                     FATANNVIFQDDLPTNLTAYVTNSAALASGGTSNSIPDSLPPKLTRFPFDEGGYSIGT
                     IALGATSSIRYVTQVLTNLPGDFDGFVHNNATVGGTNGNWTGGWTVPVRLGGLSIEKE
                     TSTTNLLEPGTNFSYTLTVANTGTVTFTGIQIEDTLPLGVTHVPGSAQIEVSGGITNT
                     VTDLFNVRTYTNINGNIPWISGWIESGESDGVAAGGVQVRADVAPAVPLEAYALQVGN
                     VGRGITRQADLSGHTSATLSFDFRRSGLDGSTDYVDVFISSNNWASSNLLVRLEGAAD
                     ETARTETNFNISAYISTNTGIRFLSSPTMGAADFVWFDNIQFTLVGNDATFAGTPPPL
                     LGQNLTLPPGGTITATFDVTVDLPPVSTQVVNMARVRADQDSTWLDSNPVTNTINATE
                     GIGMQKTSGTTNLLTAGTSVVYTIEIFNTGTVAQTGIHLEDALPPGMTFVSAELYRPF
                     LHTNEFLDIFNFQTYTNSDGNVAWTGPWVETGDDGSPTGGDIVIAVDGGSIPGQTYAL
                     RTAGTTATIRREANLAGYTNAVLWLDYRSSGLEAGEYLSVQVSGNGGGSFTEVGQIGT
                     DEDDGSYFPTSYNISAYASTGTVIRLSGGGGRGAADYVWVDNIRIAASAQNATNALPP
                     TPDLLDGYTLPPLTNMTVTVTVEVNDPLEALEFVNTATLTSDQQTTPLISSATNEAEG
                     IVGMSIAKTSLLAGTWDMGETNFYEITIENTGTVTQTGVFLTDVLPPGTVYVPGSVTV
                     TQSPPPVFTVQAFTSVGTTNFTAPDDVSRVEVLVVGGGGGGGTGGTASRGGGGAGGIV
                     HRTNYTVTGGASYTVTVGGGGGAGTAGSESVFDTLT"
BASE COUNT         1126 a         1655 c         1849 g         1115 t
ORIGIN      
        1 ctccagaatt cccatccagc ctgcatgatc tctttggagg ggcgagctct gcgagcccgt
       61 tttaatgcgc gcagaggacg cagaagtgcc aaaaaacgca aaaaacggcc tgaaaatgcc
      121 ccaaaatgcc gaaaatctgc aaaaaatggc cgtttttcgc gatttttgcg aaaaatcaga
      181 ggattttgag cttggggagg tcctggatgt caatcaggcc cgcgaggcgg ccattcgagt
      241 ccacgatgat gagatcgtca atgttgtgct tttcgtagag ggccagtaca tcaatggcca
      301 gatggtcttc agtgagcgtg accggatcgg ggctcatgat ggattcaacc ggttcgctgg
      361 cgatgttcag cgatccgccg atgtggcggc gcaggtcgcc gtcggtgatg atgcccttga
      421 cgcgcctgtc cgtgtccacg acgacacacg atccggcgcg cgcccccgtc atggccagca
      481 cggcttcctt gaccagcgtg cccattggga cactggccac gcggtttccc gtgcgcatga
      541 tgtcggaagc cttgagcaga agcgttttgc cgatggcgcc gccgggatgg agcagggcat
      601 agtcctcttt tttgaagccg cgcgcttcca gcagaaccat ggccagcgcg tcgccgaccg
      661 cgagtgtggc cgtggtgctg gcgggggggg ccatgttgaa ggggcaggcc tcacagtcaa
      721 ttttgacgga tatgatggcg tcgctgtgct tcgcgagcgt gctgttctcc tctcctgtga
      781 gggcgatcac cttgactccg aaccgcctgg caaggggcat gagttcgatg agttcgtgtg
      841 tctctccgct gtaactgagg gcgatcagga cgtcctttgc gcagagaatg ccgaggtcgc
      901 cgtgcatggc ctccagcgga tgcaggaaga cggccggcgc gccggtgctg gtgagcgtgg
      961 cggctatttt ctgcccgacg ggcaggtttt ttccgattcc ggcgatgacg attttcccgc
     1021 cgccattcag gctcctgaga atgacgccga cggctttgtc gaacgttccg ttcagattat
     1081 cccgtgcctt ttcaatgccg gcaatctcga tgttcagcac gtctctggct cggtctgcgt
     1141 agttcattgg accgaatctg acaaaagcgg gttggcgaag cgagcaaatt ttcatttgac
     1201 ccggtttggg ccccgccttg atcgcctgaa aatcatccga ctttcagcga aaatattcga
     1261 ggtaaaggca tttacttttt cagggaatat gctattttcg atttgttttt gaggggggat
     1321 tcggccggaa accttgagga accagccggc tgcctgttta tatatgaaag cgccatgcag
     1381 gaggacgggt atatggttca gttgctttga accgtgcgag gagaacttcg gctttaggct
     1441 gctgtctcct gtgttgtttt ctgccgtctg gaaacagagg cccctctgtg atctggctgt
     1501 atcttcaaac agggtccgta aatgaagaca ttgcatcata agcagcctgc atgcagaaag
     1561 actgcaatat gccgcccggg atggtggctc gcggcagccg gcttgtggct ggcaggggca
     1621 accgcctggg gggcgaatgt tctgcagact tttttcgtgc cgctgccgga ggatcagatg
     1681 cagatatccc tcaacgccat tgatgcgtac agggggtaca tcggcgatga aatgcggtcc
     1741 gcgatctcga tcgttgtggg gacggacggc acggtgatct actacgatca ctgggaggac
     1801 ggatatgagg aagacatcac caatccgacc cagttgactt cgcaggtatg gggcgacgcc
     1861 actgcgggca acggctatcc gccggggttc ccgggcgacc tgctggacgc cggcgacgtg
     1921 gtgctgcttg agagcactat cgatgtgacg cgcaacagcg ttctgatcga atacgatggc
     1981 cgcgacaagg tcgcgacgac ctttccgctt gcgatgacgc gcgccatgta tgcggtcaat
     2041 cccggcgaag ttctggcgga gtccacgggg atatacgaca taggcaccca cgacacgctc
     2101 taccgcgcac cggtgggcgt gggcaccggg accggtttcg gcaccaacga gctgttcaac
     2161 tacacggcgt tctatatcat ggccgactac gattatacgc gcatagacct tgacaaggac
     2221 aatgacggga tattcgagga gacggtctat ctggaccagg gcgaaccgta tttcatcaac
     2281 ggaggcattg tggccggggc aaccgtgcag ggaagccagc cgttccagtg ccacctggtg
     2341 acgggcgatg tggggtctac ctacgaaatg cgctggtacg agttgtggcc ggagtcgcag
     2401 tggggcactg actacttcac gcccgtgggc acgcggacga atcaaacggg cggcatcgtg
     2461 ccgtcgctgg tgtatctgtt caaccccaac gcggagacaa tcacggttca ctttgtgacc
     2521 cagaccagct cgggatcgtt cacggtgaat ccgaacagcg tgtatgacgt tttcgagatg
     2581 ccgctgaaca gcggggctcg tttttacaca acaaacggcg cgccgtttct cggagtgaat
     2641 gtgttcgaca ccacggtcgg cggagggact ctgtacggct atgtgcagca ctgccagacc
     2701 cacgactggg gcatcggcct gatgccggcc aacatgatgt cgaccatggg cgtggtgggc
     2761 tggggtccgg gttacggcac gaccggaacc ggggacaacg gcaatccgat ctggatttcg
     2821 accatcaccg gcacgactat ttacgtggac tacgacagtg atccggacac cggtccgctg
     2881 gtggacccgc agggcaaccg ctacgatgtg tccaccaacg ttacggcact ggaactggta
     2941 cagctgatcg atccgacgga cgatgaccag accggtatgc gctactacac cgtggacggc
     3001 acggtgctga tggcggcctg ggggcaggat ccgtcgcggg cgggcccggg caatccctac
     3061 ctcgatatgg gctacgtcat tcccgccttt ccaacggtgc tgtcaaagaa atttgcctcg
     3121 cttttaaacg acgtgaacgg caatggctac gcggactcgg gagacacgat cgagtacttt
     3181 atcgatgtgg tgaatgtggg tttcgccacg gccaacaatg ttattttcca ggacgatctg
     3241 ccgacgaacc tgaccgccta cgtgaccaac agcgctgcgc tcgccagtgg cgggacctcg
     3301 aactcgatcc ccgacagtct gccgccaaag ctgacgcgct ttcctttcga cgaaggcggt
     3361 tacagcatcg ggacgatcgc gctgggggcc acatcgtcga tccgctatgt cacacaggtg
     3421 ctgacgaacc tgcctggaga cttcgatggc tttgttcaca acaacgcaac cgtgggcggc
     3481 accaacggca actggaccgg cggctggacg gtcccggtgc gcctgggggg gctgtcgatc
     3541 gagaaggaga ccagcaccac gaacctgctg gaaccaggga cgaacttcag ctacacgttg
     3601 acggtggcca acaccggcac cgtgaccttc acggggatcc agatcgagga tacgcttccg
     3661 ctgggcgtga cacatgtgcc gggatcggcg cagatcgagg tgtccggtgg cattaccaac
     3721 accgtgacag acctctttaa cgtgcgaacc tacaccaaca tcaacgggaa cattccctgg
     3781 atttcgggct ggatagagtc aggagaatcg gacggggtgg cggccggcgg ggtgcaggtg
     3841 cgggcggatg tcgctcccgc cgttccgctg gaggcctatg cactgcaggt cgggaatgtc
     3901 ggccgcggca tcacgcgcca ggccgatttg tcggggcaca ccagcgcgac gctttcgttt
     3961 gacttccggc ggtcggggct cgacggttcg accgactatg tggacgtgtt tatctccagc
     4021 aataactggg catcatccaa tctgctggtg cgcctggagg gcgcggctga tgaaaccgcc
     4081 cggaccgaga cgaacttcaa tatctcggcg tatatctcga ccaacaccgg aatccggttc
     4141 ctgagttcgc cgaccatggg tgcagccgat tttgtgtggt tcgacaacat tcagttcacg
     4201 ctggtgggca acgacgccac tttcgcagga acgccgcctc cgctgcttgg gcagaacctg
     4261 accctgccgc cgggcggtac gatcacggcg acgttcgacg tgacggtgga tctgccgccg
     4321 gtttccacgc aggtggtcaa catggcccgg gtgcgcgccg accaggattc aacctggctg
     4381 gactccaatc cggtgacgaa tacgataaat gccaccgagg gtattgggat gcagaagacc
     4441 tccggcacga ccaacctgct gacggcagga acgagcgtgg tctacaccat cgaaatcttc
     4501 aacacgggaa ccgtggcgca gacaggaatc catctcgagg atgccctgcc gccgggcatg
     4561 acgttcgtgt cggcggagct gtatcgccct ttcctgcaca ccaacgagtt tctggatatt
     4621 ttcaacttcc agacctacac taacagcgac ggcaacgttg cgtggaccgg accgtgggtt
     4681 gagaccggcg acgacggcag cccgacgggc ggggatatcg ttatcgcggt ggacggtggc
     4741 tccattcccg ggcagaccta cgccttgcga acagcgggga cgacggccac catccggcgc
     4801 gaagccaacc tcgccggcta caccaatgcg gtgctttggc ttgactatcg cagttcgggt
     4861 ctcgaggccg gagaatacct ctcggttcag gtgtccggca acggcggcgg aagctttacc
     4921 gaggtggggc aaatcggcac agacgaggac gacggatcgt atttcccgac cagctacaac
     4981 atttcggcct acgcctcgac cggaacggtg atccggttgt cgggcggcgg agggcgcggc
     5041 gcggccgact acgtctgggt ggacaacatc cgcatcgcgg cctcggcgca gaacgccacc
     5101 aacgccctgc cgccgacccc ggacctgctt gacgggtaca cgctgccgcc gctgacgaac
     5161 atgacggtga cggtgacggt ggaggtcaac gacccgctcg aggcgctgga gttcgtgaac
     5221 acggccacgc tgaccagcga ccagcagacg acgccgctga tctccagcgc gaccaacgag
     5281 gcggagggca tcgtggggat gtcgatcgcc aagacctcgc tgctggccgg cacctgggat
     5341 atgggtgaga cgaacttcta cgagatcact atcgagaaca cgggaacggt cacccagacc
     5401 ggcgtgttcc tgacggacgt gctgcctccg gggacggtgt atgtgcccgg cagcgtgacg
     5461 gtcacgcagt cgccgccgcc ggtcttcacg gtgcaggcgt tcacctcggt ggggacgacc
     5521 aacttcacgg cgccggacga cgtgagccgg gtggaggtgc tggtggttgg cggcggcggg
     5581 ggcggtggca cgggcggcac ggccagccgc ggcggcggcg gcgccggcgg catcgtgcac
     5641 cgcaccaatt acaccgtgac cggcggggct tcctacactg tcacggtcgg tggcggcggc
     5701 ggcgcgggca cggctgggag cgagtcggtg ttcgacacgc tgacg
//
LOCUS       DAOQWR010000148         5736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_14318_length_5736_cov_4.694244, whole genome shotgun sequence.
ACCESSION   DAOQWR010000148 DAOQWR010000000
VERSION     DAOQWR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14318_length_5736_cov_4.694244"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /gene="thrC"
                     /locus_tag="PKK36_10180"
     CDS             complement(<1..904)
                     /gene="thrC"
                     /locus_tag="PKK36_10180"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNR94958.1"
                     /translation="MKYISTRGQSAPMGFQDAVMTGLAADGGLLIPQSIPDVTGRLQE
                     WAELPYRELAFQLTGLFTDIPPDDLKKLISLSYESFRHPEVTPLVRAGDLRILELFHG
                     PTLAFKDVALQWLGNLFEYILSRSGHHLNILAATSGDTGSAAIYGVRGRQHIHIFVMH
                     PAGRTSPTQERQMTAVLDDNVFNIAVSGTFDDCQNIMKSIFRDVEFKTRHALGAVNSV
                     NWARVLAQIVYYFYAGLKVMREEGCPEISFSVPTGNFGDILAGWYAARMGLPVKRLIL
                     ATNENDILSRFFNTGTYSLGQVVPT"
     gene            complement(907..1848)
                     /locus_tag="PKK36_10185"
     CDS             complement(907..1848)
                     /locus_tag="PKK36_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNR94959.1"
                     /translation="MLSIRHRQVFTRAALFLFLLTVFSAQAAQLPAHLKTAKDEFSVM
                     TWNLMRYAHEDRDGDGQEDDLKPQKECEAIFQVIADEQPDIVAVQEMGNPDAFAHFTN
                     GLTAKGLRYPHVEYLRRGGKQEINMAVLSRFPIAASQPITNDTYSIGPAKLPVARGFI
                     NVDIQPAPHYRFKLITAHLKAKVFHPLGQTEMRRNEARLLNKHVRQSLRKKPNLPILV
                     VGDMNDTYNSAALREVIGKDTRHLFDIRPRDQLGTVWTYYGSGDDTYSRIDYMLASRG
                     MAQQLVKQKCRASGNLLCREGSDHRPLLSVFRIPSAP"
     gene            complement(1853..3229)
                     /locus_tag="PKK36_10190"
     CDS             complement(1853..3229)
                     /locus_tag="PKK36_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR94960.1"
                     /translation="MAEMTMEEAPRKARELFEKGMAAMERGNTDYAIDIFLNTLDLEP
                     RLLKVRRFLRAAELKKAKEKGGGQMTHAIASLSGMGAMMKAKGAVKKNPVNALRAAED
                     LMKIDPLNLTFANVLVEAALAADMPEVAIQHLEILKEFYPKNVKLLNQLGKLYLDTNQ
                     PHAARDAYAAVEKLMPNDPKAVKNLKDASALASMKKGGWEEAKSYRDVMKDKDEAVRL
                     EQQSKSSKSKKDVDDLIRETAAKVQAEPNNMNYRRALADLYTRSKNYNEALKVLREAL
                     EMMGGADPQIDLAISSVRQKQFDEEIEKLRAAGDEAGAAAREQARADFLLQDAEEKVK
                     RYPNDLQFRYDLGALLYERGDLNGAIQQLQMAQRNPQRRIRALYYLAMCFKAKEQHDI
                     AMEQLEKASSELHLMDDTKKDVVYEMGIISEAMGKPEQAVAYYKEIYAVDIGYKDIAQ
                     KIDQAYGK"
     gene            3392..4414
                     /locus_tag="PKK36_10195"
     CDS             3392..4414
                     /locus_tag="PKK36_10195"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNR94961.1"
                     /translation="MKRKSRNMTGGLILAAMLALPAALMASAIARPQVQTFALGSERY
                     AQLRDVARFYNLDLQSARGSRIPMKSKWTDLQLEASSRKMWMNGMLIWLHMPTVKSRL
                     QWALSKEDMDSTLDPLLRPNLHLRSQGSRIVVLDPGHGGVDRGARGARGLEEKKVVMD
                     IAERARAHLANAGIKVYLTRSGDRALSLSERNFRANQWNADVFVSIHLNASTTAGASG
                     VETYVLAAPGYPSTNAKVGSTAARVSYRGNARNGANMILGYMLQKNLLLKTKAEDRGL
                     RRARFAVLKDINCAAALVECGFLSNAREEWLLRTDDYLDKIAQAVAQGIIEYLGTVER
                     AKVSMP"
     gene            complement(4422..4796)
                     /locus_tag="PKK36_10200"
     CDS             complement(4422..4796)
                     /locus_tag="PKK36_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR94962.1"
                     /translation="MTETKQTILIIEDEPMICRLMDRLLTKTGYEVLTAETGTEGIGK
                     FREHIEDISLVILDMSLPKLNGAEVLKILRQEKPGSKVLLSSGWVPAEVDALFADNRP
                     DGFLPKPFQTTVLLQKVEELLK"
     gene            4980..>5736
                     /locus_tag="PKK36_10205"
     CDS             4980..>5736
                     /locus_tag="PKK36_10205"
                     /inference="COORDINATES: protein motif:HMM:NF039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HNR94963.1"
                     /translation="MKKACWSVLLAIVFCVSAYAGDGTEGNPYTIAEARALSAGATEY
                     WAQGYIVGGRYDDFETPCQYDYAVSCADSASETDFANCLQVLLGKDGGRTTWGLASNP
                     GNVGKMIKFKGFRDSYGGAGNPSFEGVDNADISEVLQENQPPILASIGNKSVVWSNTL
                     SFNVTASDPVDGDSIVLSASGVPAGATFAAVTNAGAVTNTFSWPNAGPMGVYTTTFSA
                     VDNDGTSSETITITVGDGSGPSYIAFQGFEGTGA"
BASE COUNT         1106 a         1647 c         1709 g         1274 t
ORIGIN      
        1 gggtcggcac cacctgcccc agactgtagg tccctgtgtt gaagaaccgc gaaagaatgt
       61 cattctcgtt cgtggccaga atcagacgct tcaccggaag tcccatgcgt gcggcatacc
      121 agcccgccag aatgtcgccg aaattgccgg tgggcaccga aaaactgatt tcggggcagc
      181 cttcttctct catgaccttc aggccggcat agaagtaata aacgatctgc gccagcacac
      241 gggcccagtt gaccgagttg accgcgccga gggcgtgccg ggttttgaac tccacgtcgc
      301 ggaagatgga tttcatgatg ttctggcagt catcaaacgt gcccgagacg gcaatgttga
      361 agacgttgtc atcgagcacc gccgtcatct gccgttcctg tgtcggactg gtgcggcctg
      421 ccgggtgcat cacaaaaatg tggatgtgct ggcgtccgcg caccccgtag atggccgcgc
      481 tgcccgtgtc tccgctggtg gccgcgagga tgttgagatg atggccggaa cggctcagaa
      541 tgtactcgaa gaggttgcca agccactgca gcgcaacatc cttgaatgcc agcgtcggac
      601 cgtgaaacag ctcgaggatg cgcaggtcac ccgcgcggac aagcggcgtc acctccggat
      661 gacggaagga ttcatagctc agggatatga gctttttcag gtcgtccgga gggatgtccg
      721 tgaaaagacc ggtcagttgg aaggcaagtt cccggtaggg cagctccgcc cattcctgca
      781 gccgcccggt cacatcggga atgctctgcg ggatcagcag tccgccatcc gccgccagtc
      841 ccgtcatgac ggcatcctga aaacccatgg gcgcggactg accgcgtgtg ctgatgtact
      901 tcatgctcat ggcgcggagg ggatcctgaa tacactgagc agcgggcggt gatcggaacc
      961 ctcgcggcac agcaggtttc ccgacgcccg gcatttctgc ttaaccagct gctgggccat
     1021 gccgcgactg gccagcatgt agtcaatgcg cgaataggtg tcgtcgcccg aaccataata
     1081 agtccatacg gtgcccagct gatcccgggg ccgtatgtca aaaagatgcc gggtgtcttt
     1141 cccaatgacc tcgcggagcg cggcggagtt ataggtgtcg ttcatgtcgc ccacaaccag
     1201 aatcgggagg ttcggctttt ttcgcaggct ctggcggaca tgcttgttga gcagccgggc
     1261 ttcgttgcgc cgcatttcgg tctgtcccag cggatgaaac accttggcct tcaggtgagc
     1321 ggttatcagc ttgaaccggt aatgcggggc gggctggatg tcgacattga tgaagccgcg
     1381 cgcgacgggg agtttggctg gcccgatgct gtaagtgtca ttggttatcg gctgactggc
     1441 cgcgatggga aaacgactga gcacggccat attgatttcc tgcttccctc cgcgccgaag
     1501 atattccaca tggggatagc ggagtccttt ggcggtcagg ccgttcgtga aatgcgcaaa
     1561 ggcgtccggg ttgcccattt cctgcacagc cacgatgtcc ggctgttcgt ccgcaataac
     1621 ctgaaaaatc gcttcgcact ccttttgcgg cttcaaatca tcttcctgtc cgtccccgtc
     1681 gcggtcctca tgggcgtagc gcatgagatt ccacgtcatg accgagaact catccttcgc
     1741 ggttttcagg tgggccggaa gctgggccgc ctgcgcagag aaaacggtca gcaggaagag
     1801 gaacagtgcg gcgcgggtga aaacctgccg gtgacgaatt gacagcatgt ctctatttcc
     1861 cgtaggcctg atcgattttc tgcgcgatgt ccttgtagcc gatgtccacc gcatagattt
     1921 ccttgtaata ggcgacggcc tgctcgggct tgcccatggc ttcggatatg atgcccattt
     1981 catagaccac atccttcttg gtgtcgtcca tcaggtggag ttcggatgag gccttttcaa
     2041 gctgttccat ggcgatgtca tgctgctctt tggctttgaa gcacatggca agataataca
     2101 gggcccggat gcgcctctgc ggattgcgct gggccatctg cagctgctgg attgcgccgt
     2161 tcaggtcgcc gcgctcatac agcagcgcgc cgagatcata gcggaactgc agatcattgg
     2221 gatagcgttt cactttctcc tcggcgtcct gcaggaggaa gtcggcgcgc gcctgttccc
     2281 gtgcggccgc tccggcttcg tcgcctgccg cgcgcagttt ttcgatctcc tcgtcaaact
     2341 gtttctggcg gacgctgctg atggccaggt cgatctgcgg gtcggcgcct cccatcatct
     2401 ccagcgcctc gcgcagcacc ttgagcgcct cgttgtagtt tttggagcgc gtgtacaggt
     2461 cggccagcgc ccggcggtag ttcatgttgt tcggctccgc ctgcaccttg gccgcggttt
     2521 cgcggatgag atcatccaca tccttcttgg acttggaaga cttgctctgc tgttcaagcc
     2581 gcaccgcttc atccttgtcc ttcatcacat cgcggtagct cttggcctcc tcccatccgc
     2641 ccttcttcat ggaggcgagg gccgaggcat ccttcaggtt cttgacggcc ttcgggtcat
     2701 tgggcatcag cttttcgacc gcggcatagg catcacgcgc ggcgtgcggc tgattggtgt
     2761 ccagataaag cttcccgagc tggttgagca gtttgacgtt cttcggatag aactccttca
     2821 gaatttccag atgctggatc gccacctcgg gcatgtctgc ggccagtgcg gcttcgacga
     2881 gcacattcgc gaacgtgagg ttcagggggt cgatcttcat cagatcctcg gccgcccgca
     2941 gcgcgttcac cggatttttt ttcacggccc ccttggcctt catcatggcg cccatgccgc
     3001 tcagggaggc gatggcgtgg gtcatttgcc cgccgccctt ttctttcgct ttcttgagct
     3061 cggcggcgcg gaggaagcgg cgcaccttca gcaggcgcgg ctccaggtcg agcgtgttca
     3121 ggaatatgtc gatcgcataa tccgtgttgc cgcgctccat ggcggccatg cctttttcaa
     3181 aaagttctcg ggccttgcgg ggggcctctt ccattgtcat ctctgccatg ctgacgggct
     3241 cctttcaaaa aatgtcttta ttcatgactt tacgaaaaca ggcggaagct ggcaactgct
     3301 ttattgatga acaaaacgcc gccgatattt cttctggtct cggcatgggc atccacctag
     3361 aatgcgcgcc atggtcatat ccggataaaa gatgaaacgg aagagcagaa acatgacagg
     3421 ggggctgatc ctggcggcga tgctggcgct gccggccgcc ctgatggcat ccgccattgc
     3481 ccgcccccag gtgcagacgt ttgcgctggg ttcggaacgc tatgcccagc tgcgggacgt
     3541 cgcacgcttc tacaatcttg atctgcagtc cgcacgggga agccgcatcc ccatgaaaag
     3601 caaatggacc gatcttcagc tggaggcctc ctcacggaaa atgtggatga acggcatgct
     3661 gatctggctt cacatgccca cggtgaaaag ccgcctgcag tgggctttga gcaaggagga
     3721 catggattcc acgcttgacc cgctgctccg gcccaatctg catctgcgca gccagggttc
     3781 gcgcattgtg gtgctggatc ccgggcacgg cggagtggat cgcggcgcgc gcggcgcgcg
     3841 cggtctcgag gaaaagaaag tggtgatgga cattgcggag cgcgcccggg cccatctggc
     3901 gaacgcgggc atcaaggttt atctgacccg gagcggcgac cgcgcgctga gtctttcgga
     3961 gcgcaatttc cgcgcgaacc agtggaacgc tgatgtgttc gtcagcattc atcttaatgc
     4021 cagcaccacg gcgggcgcca gcggggttga aacctatgtg ctggcggcgc cggggtatcc
     4081 ctccaccaat gcgaaagtgg gttccaccgc cgcgcgtgtc agctacaggg ggaatgcccg
     4141 caacggggcc aacatgattc tgggctacat gctccagaag aaccttcttc tgaaaacaaa
     4201 ggcggaagac cggggcctgc gccgggcacg ctttgctgtt ctcaaggata tcaactgcgc
     4261 cgccgccctg gtggaatgcg gatttctgtc caacgcgcgc gaggaatggc tgctccgaac
     4321 cgacgattat ctcgacaaga tcgcgcaggc cgtcgcgcag ggaatcattg aatacctggg
     4381 gacggtggag cgggccaaag tatccatgcc ttaagcggaa gctacttcag cagttcttcg
     4441 actttctgaa gaagaaccgt ggtttgaaag ggcttgggca ggaatccgtc cggccggttg
     4501 tcggcaaaca gcgcatcgac ctcggccggc acccatcctg agctgagcag caccttgctc
     4561 cccggttttt cctgccgcag gattttaagg acctcagcgc cattcagctt tggaaggctc
     4621 atgtcaagaa tcaccaggct gatgtcctca atgtgctccc ggaatttccc aatgccttcc
     4681 gtgccggttt cggcggtcag gacttcatag cccgttttgg tcagcaggcg gtccatcagg
     4741 cggcagatca tcggctcgtc ctcgatgatc agaatggtct gttttgtttc tgtcatttcc
     4801 atctattgat ttataaggcc cacctttata gtaaatcaga gactccggcg ccagcggcaa
     4861 tccgaaaaaa gattttttat tattctgttt gctttcacgc aacccggaga atacaattcc
     4921 gcaaattgga cgagtctaca tctgtgtcaa aaagtgttgt ggaaagaatg gagaagtgca
     4981 tgaaaaaagc ctgctggtct gtcttgctcg cgattgtatt ctgtgtttca gcttatgccg
     5041 gtgacggcac cgaagggaat ccctatacca tagcggaggc gcgggcgctg tccgccggcg
     5101 ccacggaata ctgggcgcag ggatacatcg tcggcggccg ctacgacgac ttcgagacac
     5161 cgtgccagta tgattacgcc gtcagttgcg ccgattccgc ctccgagacg gattttgcca
     5221 actgcctgca ggttctgctg ggaaaggatg gaggccgcac gacgtggggc ttggccagca
     5281 atccgggcaa tgtcggcaag atgattaaat tcaagggatt tcgggattcg tacggcgggg
     5341 caggcaatcc gagcttcgaa ggtgtggaca acgcggatat ttccgaagtc ttgcaggaga
     5401 accagccgcc gatccttgcg tccatcggca acaaatcggt agtttggagc aacacgcttt
     5461 cctttaatgt gaccgcctct gatccggtgg acggcgattc gatagttctt tcagcttcag
     5521 gtgtgcccgc aggcgcaacc tttgcagctg ttaccaacgc tggcgcggtg accaacacct
     5581 tctcctggcc caatgccggg cccatgggag tctatacaac aaccttctcg gccgtggaca
     5641 acgacggaac cagcagcgaa acaatcacca tcacggtggg tgacggcagc gggccctcgt
     5701 acattgcttt ccagggtttc gaaggcacgg gggctg
//
LOCUS       DAOQWR010000149         5685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_14476_length_5685_cov_5.715631, whole genome shotgun sequence.
ACCESSION   DAOQWR010000149 DAOQWR010000000
VERSION     DAOQWR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14476_length_5685_cov_5.715631"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5685)
                     /locus_tag="PKK36_10210"
     CDS             complement(<1..>5685)
                     /locus_tag="PKK36_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR94964.1"
                     /translation="ADNCLVVGVKTMGGSTAFGVNNGISNTFVNCMAANASSRGLYSR
                     KGSPTIWRSGVLWSNAVGVYVDDGKLTFENSVIGAFGSGNYAFFNKESAATNQIMSDY
                     NNIWLQDDALAGYYDAYPSTSPDDRVFVTLSRWTRDTGLDQHSLSHDPGFAAAVSNDF
                     HLLRPAGRFIPGSGFETNEAEGLSVLVDAGSPASAYTNEPAPNGMRINIGMYGNTVQA
                     SQTPTNSRLTAVSLNDGGRVEGIRDLVWVASGDVTGHTVFIEFSANGGVTWTTLVTGV
                     SASAGVYSWDTTAYPSTPLGVWRITSEDDASVTDQTETLFAVRNGPLNFYVNDVVTNG
                     DVFCSSTGAVEHTGVFPNDPKSDVQDVLDTYDLEPGDTIWVDTGGYTLTSSITMGIFD
                     MGEPTNRVVVRGSTNMAAGGSVLGNFGFNLTGLKGIELRDMKITNSAVGVYVSGSSGM
                     LLHGLEAVGCTNGFQILGSSSEDVTLRQCAARGGQQGLYVQTSASVTWENGLLWSNAY
                     GVYLNQASLDFRNSVVGALKAGQYAFYYRPGSLTSDYNVVYLTNGAYAGNFVPTSGGT
                     ITTYENVSRWVRGFGFDRHSLSHAPGFYSAASNDFHALSPAGRYVSGSGYMTNAAEAL
                     SILVDSGDPLSDVADETLPNGSRINIGRYGGSGQSSLSPTNARLIAVSLNDGGRAEGV
                     KELTWVASGDATGHTVSIEFSHDAGVTWTTLVSAVTATDESWTWDTTQVPSTPVGVWR
                     VTSVADPSVSDTNDTLFAVRNDPLFFYVNDSSTNGDVYCSAAGTNSNTGAFSNAPKAS
                     VQDVISTYDLEPGDTIYVDTGNYPLTASIQPGVFDAGSATNRISLIGSTNYASGGTRF
                     TGYGIDMNAVGGWALRHFAVSGAAKGVRLFQADDVLVEWVQTYGGVFGFEINDCERSV
                     LRHCAASGNSAQGVYNRKGYDTIWDHGVVWSNLYGIYMDEGSMQVQNTVIGAFGSGRR
                     AYYYRERSVSDVFDCDYNNIYLTDGAYAGYLDSLAGTDYDIIYENASRWIRDFGVDRH
                     SLSHDPLFADAANRDFHLQTQAPDGRYVHGLGFATNDLVTSPLIDAGNPLSDASSEPP
                     PSGARVNIGLYGNSDQASQTPTNSMLTVVSLSSGGRVEGISDLVWVASGDVTGHTVTI
                     EYSSNGGLTWTTLVSGVDASISAWTWDTTQFEDSAIGQWRITSEDDPAVSDTNDVLFA
                     VRNAPLAFYVNDLSDSGDIYTSVIGNSTNNGLSPLSPQISVQYILDAYDLEPGDTIYV
                     DTGSYTLTSEIIATLQDSGAATNFVSIQGSTSDVSRTIFTAFGVANAFTLDDVWGFVL
                     RNMTIRNASTAGVYMNRAWHSGAEWVICENSGRDGFRVRESVSTFLNHCIARASIRHG
                     VWNESSPGTEWINGIVWSNVNGINIEGGSIKVENSVLSALNSQQQAYRYVAGVLTSDY
                     NNIYRKNGGFAGIVVAEGIYTTKYDSASSWTATFGYDGSSLSSEPGFWNADGGDYHLR
                     SPAGRYQPGSGFVTNPLEGASLLIDAGNPSTPVGDEPEPNAGRINIGLYGGTGQASMT
                     YTSAVLSVSSFDDGGYVTGTVSLVWLARGAATNHTVQIDFSANGGVAWTTLVTGIDAT
                     LGTWTWDTTAQMSTPLGVWRVTSIEEPLVSDQNAVFFAVRNAPFHFYVNDNSTTGDVY
                     TLVKGSDAYSGLTVSDPMASLKTVIDTYDLEAGDTIWVDTGTYPLSGDITIGTLDSGT
                     TNIPVMIRGSTNAVYGGSVLDRQVPGGGTAGIRFNGALGVVIKDLGVKRAENGVVFEN
                     ATRACRAEGVTSFDNGSAGFSVSLSKDILMQGCLAINNSTYGVRASGGVMPTSYLWEN
                     GVIWQSGTAVYMTSSTANQFRNSLLQGSGAGARVYDIGLGASLVSDYNNFAIT"
BASE COUNT         1295 a         1715 c         1460 g         1215 t
ORIGIN      
        1 tgtgatcgcg aaattgttgt agtcagaaac cagggaagca cccaacccga tgtcatatac
       61 tcgcgcgccc gcaccactgc cctgcagaag gctgtttctg aactggtttg cggtgctgct
      121 cgtcatataa accgcagttc cgctttgcca gatcacgccg ttttcccaca aatatgaggt
      181 tggcattaca ccgccagaag cgcggacgcc gtaggtcgaa ttgttaatgg caaggcagcc
      241 ctgcatcaga atatccttcg agagtgaaac cgaaaatccg gcggatccgt tgtcaaacga
      301 agtcactccc tcagcccggc aggcacgggt ggcgttttca aaaaccactc cgttttcagc
      361 gcgcttcacg ccgaggtctt tgatcacaac cccaagtgcc ccgttgaaac ggattcccgc
      421 cgttcctccg ccgggaacct gccggtccag aacactgccg ccgtatactg catttgtgct
      481 gcccctgatc atcaccggga tattggtcgt cccgctgtcc agagttccta tggtaatgtc
      541 tccggacaga ggatatgtcc ccgtatccac ccaaatcgtg tccccggcct caaggtcgta
      601 tgtatcgatc accgttttca ggctggccat gggatcggag accgtcagcc ctgaatacgc
      661 atccgaacct tttacaaggg tatacacatc gcccgttgtt gagttgtcgt tcacgtagaa
      721 atggaacggc gcattccgga cagcaaagaa tacggcgttt tgatcggaaa ccagcggttc
      781 ctcgatactg gtaacacgcc acacaccaag cggagtggac atttgcgcgg tggtatccca
      841 cgtccatgtg cccagcgtgg cgtcaattcc ggtgaccagc gtagtccatg ctaccccgcc
      901 attggccgaa aaatcgattt gaacggtatg attcgttgcc gccccgcgag ccagccagac
      961 cagagaaacg gttcccgtga catatcctcc gtcatcaaac gacgaaacgc tcagcaccgc
     1021 gcttgtgtag gtcatggagg cctgtccagt gccgccataa agcccgatgt taattctccc
     1081 agcgttgggt tctggttcgt cccccaccgg agtggatgga tttccggcgt caatcagaag
     1141 ggatgcgccc tccagaggat tcgtgacgaa gcccgagccc ggctgataac ggccggccgg
     1201 gctccggagg tgatagtccc ccccgtccgc attccagaaa ccgggctcac tggaaagaga
     1261 acttccatca tatccgaatg tggccgtcca cgaggatgcc gaatcatact tcgtggtgta
     1321 aatgccctcc gccacgacaa tgcccgcaaa tccgccgttc ttgcgataaa tgttgttata
     1381 gtctgatgtc aggacaccag cgacatagcg ataggcctgc tgctgagaat taagcgcgct
     1441 caaaaccgaa ttttcaacct taatgctccc gccttcaatg ttgattccgt ttacattgct
     1501 ccaaacaatc ccgttgatcc actcggttcc cggcgaactc tcattccaga cgccatgccg
     1561 gatgctggcg cgggctatgc aatgattcaa aaaagttgaa acactctctc ggactcggaa
     1621 gccgtctctt ccgctgttct cgcaaataac ccattcggcc cccgaatgcc atgccctgtt
     1681 catataaacc ccggcggtac tggcattgcg aatggtcata ttgcgcagca caaaacccca
     1741 aacatcatca agcgtgaagg cattcgccac gccaaaggcc gtgaagatcg tccggctcac
     1801 atcgctggtg cttccctgta ttgaaacgaa attggtcgcc gcgcccgaat cctgaagggt
     1861 tgcgataatc tccgaagtca gcgtgtaact gcccgtgtcc acatagatcg tgtcgcccgg
     1921 ctccagatca taggcatcca gtatatattg aactgatatc tgcggcgaga gagggctcag
     1981 tccgttattc gtgctgttgc caatcaccga ggtatagata tcgcctgaat cgctcaagtc
     2041 attcacatag aatgccagcg gggcattgcg caccgcaaag agcacatcgt tggtatccga
     2101 cacagccgga tcatcctcac tggtgatccg ccactgtcct atcgccgaat cttcaaactg
     2161 cgttgtgtcc catgtccacg cgctgatgga cgcgtccacg ccggatacga gcgtggtcca
     2221 tgtcagcccc ccattggacg aatattctat ggtgacggta tgtccggtca cgtcacctga
     2281 agccacccaa acaagatcgg aaatcccctc aacacgaccg ccggaactga gcgagacgac
     2341 ggtcagcatt gagttggtcg gtgtctggct ggcctgatca ctgttcccgt aaaggcctat
     2401 gttcaccctt gcaccggagg ggggcggctc ggacgaggcg tccgacagcg gatttccggc
     2461 atcaatcaaa ggagacgtca ccaagtcatt cgtggcgaaa cccagaccgt gcacataacg
     2521 accgtccggg gcctgcgtct gcaaatggaa atcgcgattt gcggcatccg cgaacagggg
     2581 atcgtggctc aaactgtgcc ggtctacacc gaagtcgcgt atccagcgcg aggcattctc
     2641 ataaataatg tcataatccg tacctgcgag ggagtccaga tatccggcat aggcgccgtc
     2701 cgtcagataa atattgttgt aatcgcagtc aaacacatca gatacggaac gttcacgata
     2761 ataataggca cggcggcccg atccaaacgc cccgatcact gtattctgaa cctgcattga
     2821 gccttcatcc atataaatcc cgtaaaggtt cgaccagacc acaccgtggt cccatatcgt
     2881 gtcatagccc ttccggttat agactccctg cgccgaattg ccggacgctg cgcagtggcg
     2941 cagaacgctt ctttcgcagt catttatctc aaagccaaac acgccgccat aagtctgcac
     3001 ccattcaacc agcacatcat cagcctggaa cagccgcacg ccctttgccg ctccggaaac
     3061 cgcgaaatgc cgcagcgccc atccgcccac tgcattcatg tcaatcccat aacccgtaaa
     3121 ccgggttccc ccggaggcat aattcgtgct gccgatgagg ctgattcgat tggttgccga
     3181 gcccgcatca aaaacacccg gctggatgct ggccgtcagc ggatagtttc ctgtgtccac
     3241 ataaatagtg tcgcccggct caaggtcata ggtgctgatc acatcctgca ccgaagcctt
     3301 gggtgcattt gagaaagccc cggtattgga attggtgccc gctgcagagc agtatacatc
     3361 cccgttggtg gaggaatcat tgacatagaa gaacagcgga tcgttgcgga cagcaaaaag
     3421 ggtgtcattg gtgtctgaaa cagaaggatc cgcaacgctg gtgactctcc acactcccac
     3481 aggcgtagag ggcacctgcg tggtgtccca tgtccaggat tcatccgtgg cggtcacggc
     3541 cgagaccagc gtggtccacg tcaccccggc atcatgcgaa aattcgatgc tgaccgtatg
     3601 accggtggca tcaccggatg ccacccatgt cagctctttc actccctcgg cccggcctcc
     3661 gtcattgagc gatacggcaa tcaggcgggc attcgtcgga gaaaggctgg actgtccgct
     3721 tccgccataa cgcccgatat tgattcttga tccgttgggc agcgtttcgt ccgcaacatc
     3781 actcagcgga tcaccgctgt ccaccagaat ggacagggct tcggcggcat tcgtcatata
     3841 tccggaaccc gacacatagc ggccggcggg gctcaaagca tggaaatcat ttgatgcggc
     3901 actgtagaat cccggcgcat gcgacaggct gtgtctatca aatccaaagc cccggaccca
     3961 ccgggagacg ttttcatatg ttgttatcgt gcccccggag gtaggcacaa agtttcctgc
     4021 ataggctcca ttggtcagat agacaacatt gtagtcggag gtcagagacc cggggcggta
     4081 ataaaatgca tactgtccgg ctttaagcgc cccgaccact gaattgcgga aatccagact
     4141 ggcctgattc agataaaccc cataggcatt ggaccagagc aggccgtttt cccacgtcac
     4201 gctggcagac gtttggacat acagcccctg ctgcccgccc cttgccgcgc actgccgcag
     4261 cgtcacatcc tcagaggaac ttcccagaat ctggaaacca ttggtgcaac caaccgcctc
     4321 cagaccgtga aggagcatcc cggaggagcc gctgacataa acgcccaccg cgctgttggt
     4381 aattttcatg tcgcgcagtt ctatcccctt gagccccgtc aggttaaagc cgaaattgcc
     4441 cagcacagac ccgccggcgg ccatgttggt tgaaccccgg accaccacac ggttcgtcgg
     4501 ctcacccatg tcgaatattc ccatcgtgat actgctggtc agtgtgtaac cgcccgtgtc
     4561 cacccagatt gtgtcgccgg gctccagatc ataggtgtcc agcacatcct gcacatctga
     4621 cttgggatca ttcgggaaca ctcccgtgtg ctctaccgca ccggtgctgg aacagaacac
     4681 atcgccgttg gtgaccacat cattgacata gaaattcaac gggccgtttc gcacggcaaa
     4741 cagcgtttcc gtctggtcag taacggaagc gtcatcttca ctcgtaattc tccatacgcc
     4801 aaggggcgtg gatggataag ctgtggtatc ccatgaatac acgccggcgg aggcagaaac
     4861 gcctgtgacc agagtggtcc atgttactcc gccattggcg gaaaattcga tgaatacagt
     4921 gtgcccggta acgtcgcctg aggcaaccca gaccagatcc ctgatgccct ccacgcgacc
     4981 gccgtcattc agcgaaaccg cggtcaggcg gctgttggtg ggggtctgac tggcctgcac
     5041 ggtattaccg tacatgccga tattaattct catcccgttc ggagcgggtt cattggtata
     5101 tgccgacgcc gggctgccgg catccaccag cacggacaat ccttccgcct cattggtctc
     5161 gaatccgctc ccgggaataa atcggccagc gggtctgagc agatggaaat cgttgctgac
     5221 ggccgccgca aaccccggat catgactaag gctgtgctga tccagaccgg tgtctctcgt
     5281 ccaccgcgaa agtgtaacaa aaaccctgtc gtcaggagat gttgacggat aggcatcgta
     5341 gtatccggcg agggcatcat cctgaagcca gatattgttg tagtcggaca ttatctggtt
     5401 ggttgcggca ctttctttgt tgaaaaatgc gtaattcccg ctgccgaacg cgccaatcac
     5461 gctgttctcg aaggtcagct ttccgtcatc cacatatacc cctaccgcat tcgaccagag
     5521 aacgccgctc cgccagatgg tgggggaacc cttgcggcta tacaaaccgc ggctggaagc
     5581 gtttgccgcc atacagttca caaatgtatt gctgatcccg ttattgacac caaaggccgt
     5641 gctgcccccc atggtcttaa ctcccaccac gaggcagttg tcagc
//
LOCUS       DAOQWR010000150         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_14713_length_5612_cov_5.298542, whole genome shotgun sequence.
ACCESSION   DAOQWR010000150 DAOQWR010000000
VERSION     DAOQWR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14713_length_5612_cov_5.298542"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKK36_10215"
     CDS             <1..955
                     /locus_tag="PKK36_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94965.1"
                     /translation="KHWKKLALGLVVLLVLCIAPDARGFVREGRAYIQAVYAPNWNRL
                     SSISRVADKITNPLLGSDPLPRPFLEAIGTNTTAFYPLELSLAAFNDVQFRPMPVLQA
                     YTAYTPYLDGWNADFFSEDMTAPAFIVLSLRAVDGRWPLIESPLAWNAISENYEFHLM
                     DPPFLLLKRRSVPREVAYRALSDSECGTMQEITLPETAGWVFITAEMRLNLRGRIAKL
                     FYRVPEVTIRAAFENGLVVDGRCIPENLRSKTLISFLPLPFELEETAAFLRSGHEPDR
                     VKQIALGGPGLAYYRRTMKVTLYEDRILNGAAASNEHVADE"
     gene            948..2333
                     /locus_tag="PKK36_10220"
     CDS             948..2333
                     /locus_tag="PKK36_10220"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HNR94966.1"
                     /translation="MNKQADWIRPEIIFLVTAVLVGMACLILTPPFQVADEPSHFFRI
                     VQIAQGGIIGERRGADSGGMIPAALLAMEDYFYDGNPVSGEGKWDPSKWAGIKPYLTE
                     RADLSDREFRDFRTSILYAPVVYLPQVTGVWLAGILKLPPLWMLYAGRLLALAVFICL
                     VYIAIRLTPICPWGFTVLALLPAVMFQAASLSGDSMTMAVLFLFTALALHYAFTPTVV
                     TRKDLILLIALGVMIGLCKSVYILSLGLLFMIPPAKLGGQKRYWTFCLGSVGLALLAA
                     ALWSVLMGKAYVRLFDDIDEWAQIAFILQEPLVFINMLLGRILRSVHYLYLGGGLGLL
                     NEFVGLLGWYAVCVYVGQTSFAVLFWCAIAEKNDKVVIREWHRWCVAGILAGTVLLVA
                     AANYIIWMPPGSPALALFGRYFHPIAPMAFLLFHNRSFRIQCEDKWRVFIPVYFAGMA
                     LATVYVIAKIY"
     gene            2338..3801
                     /locus_tag="PKK36_10225"
     CDS             2338..3801
                     /locus_tag="PKK36_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNR94967.1"
                     /translation="MPDQQKIGIFIVAYNAESRIAQTLARIPQAVWAQITEAYVVDDC
                     STDETVQHAMRLKDVYPKLRVLRSRMNQRYGGNQKIGFQYAIDRGFDILVTLHADGQY
                     APEILPEILAPVLENRADIVLGSRMIRRQGAGDGSMPLYKYGGNLLLTRILNRLAGMN
                     LHEFHTGYRAYRMRFLESVPFWDNTDDWHFDTEILLQGLARQASIAEIPVPPCYDHSI
                     RRANGLIYAVHCIQTTLRYAMHRNGICYSRNYDLNASGSKYSSKFADPYSSHTLLYRH
                     LEAQGLAGKTVLELGVGDASLTQRLAEQGTVVDCIELNSSDARTAEKYARQVWNEALD
                     FTQLDQVKERYDIVIAADILEHLLYPEEILSKLKACVKKGGLLLVSLPNVANVYVRLN
                     LLLGRFPYHSKGLLDRTHLHFYTRKSAERLVSRTGWEIAGREYSSIPVAMVFPFLRKR
                     GFRFFLHALHGLTMLFRGLFCYQFLLYCRNPNESDLL"
     gene            3933..5297
                     /locus_tag="PKK36_10230"
     CDS             3933..5297
                     /locus_tag="PKK36_10230"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNR94968.1"
                     /translation="MRMEKDSLGELQVPAGVYYGIHTVRSLKNFGISGEAMPLELIYS
                     MVRLKRACAKANAGLGLLSGDKASAICKACDRVLEGQFDAEFGIDVFQAGSGTSSHMN
                     VCEVLANIACEELGGKKGDRSLVHPNDHVNMGQSTNNIFPSAVRMAAVDLSRGLETAL
                     LRLAEKLGEKEQEFKSVIKSGRTHLQDAVPVTLGQEFGAWRRAVQKASQRVGRGREAL
                     LELGVGGNAIGTGLNTPKTFRSAILKELKGEYRLPENGIEATQFMTDLGEFSSMLKLA
                     ALDMHKIANDLRLLGSGPNTGLGEIVLPPVEPGSSIMPGKINPSICEAVNMACIQVVG
                     QDAAVCMACGAGQLELNTHMPVIGANLVKMLHLLSGAARSLADQCVCGLTANERVCRR
                     NFENSASLATVLNPALGYDRVAELVKESISSGRGLKELVLARELLDEAGWDALMKNCT
                     GPLP"
     gene            5364..5440
                     /locus_tag="PKK36_10235"
     tRNA            5364..5440
                     /locus_tag="PKK36_10235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5398..5400,aa:His,seq:gtg)
BASE COUNT         1111 a         1467 c         1677 g         1357 t
ORIGIN      
        1 gaagcactgg aaaaagctgg ctctcggcct cgtggtgctg cttgtgttgt gcattgcgcc
       61 tgatgcacgc ggatttgtcc gggagggcag ggcgtacatt caggcggttt atgcccccaa
      121 ctggaaccgg ctttcgtcca tatcgagggt ggccgacaag atcacgaatc cgcttttagg
      181 cagcgatccg ctgccccggc cgtttttaga agccatcggc acgaatacca cagcttttta
      241 tccgctggag ctgtctcttg cggcattcaa tgatgtccag ttccggccca tgccggttct
      301 tcaggcctat acggcctaca cgccgtatct ggacggctgg aatgccgatt ttttttcaga
      361 ggacatgacg gcccctgcgt tcattgttct gagtctcagg gcggtggatg gccgctggcc
      421 gctgattgaa tcgccccttg cttggaatgc catcagcgag aattacgaat ttcatctgat
      481 ggacccgcct ttcctgctgc tgaagcggag gagcgttccg agagaagttg cataccgcgc
      541 gctttctgat agcgagtgcg gaacaatgca ggaaatcacg ctcccggaaa ctgccggatg
      601 ggtgtttatc acggcagaga tgaggttgaa tctgcggggc aggatcgcca aactgtttta
      661 tcgcgtgcct gaggtcacca taagagcggc tttcgaaaat ggtctggttg tggacgggcg
      721 ctgcatcccc gagaacctgc ggtcgaaaac gctgatttcc ttcctccctc tgccgtttga
      781 actggaggaa acggccgctt tcctgcgttc cgggcatgag ccggaccgcg tgaaacagat
      841 tgccttgggg gggcccggtc tcgcatatta cagaagaacc atgaaggtga ctttgtatga
      901 agatcgaatt ctgaatggag ccgctgcttc aaatgagcat gtcgcagatg aataaacaag
      961 cggactggat caggccggaa atcatatttc tggtgacggc agttctggtc ggaatggcct
     1021 gcctgatcct gacgccgcct ttccaggtgg ctgacgagcc cagtcatttt ttccgaatcg
     1081 tgcaaattgc tcagggcgga attataggcg agcgcagagg ggcagattcc gggggcatga
     1141 ttcccgcggc gctgcttgcc atggaggact atttttatga cgggaatccg gtgtcgggag
     1201 agggcaagtg ggatccgtca aagtgggccg gcataaagcc ttatcttact gaacgcgcgg
     1261 acctgtctga tcgggaattc cgggatttcc ggacctcaat actctatgcc cccgtggtct
     1321 atctgcctca ggtcacgggc gtgtggctgg ccggaatatt gaagctcccg cccctgtgga
     1381 tgctgtatgc cggtcgtttg cttgcactcg ctgtttttat ctgcctggtg tatatcgcaa
     1441 ttcgcctgac gcccatttgc ccctggggtt tcacggttct ggcgttgctg cccgccgtga
     1501 tgtttcaggc ggcttcgctc tccggggatt ccatgacgat ggctgtgctg tttcttttca
     1561 ccgctttggc cctgcactat gccttcaccc cgactgttgt cacgcggaaa gacttgattc
     1621 tcttaattgc gctgggggtg atgatcggcc tgtgcaaatc cgtatacatt ctttcgctcg
     1681 gtcttctgtt tatgattccg cccgcgaagc ttggcggaca gaaaaggtac tggacatttt
     1741 gtcttggctc ggtcgggttg gcgctgctgg ccgccgcgct ttggtccgtg ctgatgggga
     1801 aggcctatgt gcggcttttt gacgatattg atgaatgggc gcagattgcc ttcattctgc
     1861 aggaacccct ggtgttcata aacatgctgc tgggccggat tctgcggtct gtgcactatt
     1921 tgtatctggg aggagggctg gggctgctga atgagtttgt ggggctgctg ggctggtatg
     1981 ccgtctgcgt ttatgttggt cagaccagct ttgccgtgct tttctggtgc gcgattgcgg
     2041 agaagaacga caaggttgtg atccgtgagt ggcaccgctg gtgcgtggcg ggcattctgg
     2101 cgggaacggt tctgcttgtt gccgccgcca attacatcat ctggatgccc ccggggagcc
     2161 cggccctggc cctgttcggg cgttatttcc atccgatcgc ccccatggct tttctgcttt
     2221 ttcacaaccg gagtttccgg atccagtgcg aggacaagtg gcgggtgttt attcccgtct
     2281 atttcgcggg aatggctctg gccacggtgt atgtaatcgc gaagatctat tgatgccatg
     2341 cctgatcagc aaaaaatcgg aatatttatc gttgcctaca acgcggagag ccgtatcgcg
     2401 cagactctgg cgcgcatacc acaggccgtt tgggcgcaga taaccgaggc atatgtcgtt
     2461 gatgactgca gcaccgacga gaccgttcag cacgccatgc gcctgaagga tgtctatccc
     2521 aagctgcgcg tattgcgcag ccgcatgaac cagcgttacg gggggaacca gaagatcgga
     2581 ttccagtatg caatcgaccg cggttttgac attcttgtta ccctgcatgc ggacgggcag
     2641 tatgccccgg aaatcctccc ggaaattctg gctccggttc tggagaatcg ggcggatatc
     2701 gtgctgggtt cgcgcatgat ccgcaggcag ggggcagggg atggcagtat gccgctttat
     2761 aagtatgggg gcaaccttct gcttacgcgg attctgaatc ggcttgccgg catgaatctt
     2821 catgagttcc acaccggtta tcgcgcgtac cgtatgcggt ttcttgagtc tgttcctttc
     2881 tgggacaata ccgatgactg gcactttgat acagagattc tgctgcaggg actggcccgg
     2941 caggcgagca ttgcggaaat cccggttccc ccctgctatg accacagtat ccggcgggcc
     3001 aatggactga tctatgccgt tcactgcatc cagaccacgc tgcgttatgc gatgcacagg
     3061 aatgggatct gctattcccg caactatgac ttgaatgctt cggggagcaa gtactccagc
     3121 aaattcgccg atccctattc gagtcatact ttgttgtaca gacatctcga ggcgcaaggt
     3181 cttgcgggca agacggtgct ggaactgggg gtcggcgatg catccctcac acagcgcctt
     3241 gccgaacagg ggacggtcgt ggactgcatt gaactgaact cctccgatgc ccgcaccgcg
     3301 gaaaagtacg cgcgacaggt ttggaatgaa gcgctggact tcacccagct ggaccaggtg
     3361 aaagagcgct atgatatcgt tattgccgct gatattctcg aacacctgct ttaccccgag
     3421 gagatactct ccaagctgaa agcctgcgtg aaaaagggcg gactgctcct cgtgtcgctt
     3481 cccaatgttg ccaacgtgta tgtccgtctg aaccttctgc ttggccgttt cccataccat
     3541 agcaagggac tgcttgacag gacccatctg cacttttaca cccggaagtc tgctgagcgt
     3601 cttgtcagca ggacgggatg ggaaatagcg ggccgggagt attcctccat accggtggcg
     3661 atggtctttc cttttctgcg gaagcgtgga ttccgtttct tcctgcatgc gctgcacggc
     3721 ctcacgatgc ttttccgggg gttgttttgt taccagttcc ttctctattg cagaaatcca
     3781 aatgaatctg atcttttata ggtcatggtt tctagccgtt ttgtgccgcc ccttttcctt
     3841 caaccggtcg ctccgtgatg aactttcccc aattattttg tggagagtgc cgtgcgaaaa
     3901 gttcaatgat ggagcgtgaa acggagcagg ttatgagaat ggaaaaagat tcactgggcg
     3961 agctgcaggt gccggccggg gtttattacg ggatccatac ggtccggtcg ctgaaaaact
     4021 tcggtatttc gggcgaggcc atgcccctgg agctgattta cagcatggtt cgcctgaagc
     4081 gcgcctgcgc gaaggccaat gccggactgg gactgctttc cggagacaag gccagtgcca
     4141 tctgcaaagc ctgtgaccgg gttctcgagg gtcaattcga cgcggaattc ggcattgatg
     4201 tctttcaggc cggctcgggc acatcgtccc acatgaatgt gtgcgaggtg ctggccaata
     4261 tcgcctgcga ggagctgggc ggcaaaaaag gcgaccgcag tctggttcat cccaacgatc
     4321 atgtgaacat ggggcagtcc acgaataata tctttccttc cgccgttcgg atggccgctg
     4381 ttgacctgtc gcgcggcctc gagaccgctc tgctgcgtct tgcggagaag ctgggcgaga
     4441 aggagcagga attcaaatcc gtgatcaaga gcggccgcac acatctgcag gatgccgttc
     4501 ccgtgacgct gggccaggaa tttggcgcgt ggcgtcgggc ggtgcagaag gcgtcgcagc
     4561 gcgtcggccg cggccgcgag gcccttcttg aactgggggt gggcggcaac gccatcggca
     4621 caggcttgaa caccccgaag acttttcgtt ccgcaatttt aaaggaattg aagggcgaat
     4681 accggttgcc tgaaaacggc atcgaagcca cgcagttcat gaccgacctg ggcgagtttt
     4741 catccatgct gaaactcgcg gcgctggata tgcacaagat cgcaaacgat ctgcgcctgc
     4801 tgggttccgg gcccaacact ggcctgggcg agattgtcct tccgccggtg gagccgggtt
     4861 cgtctattat gcccggaaaa attaatccca gcatctgcga ggcggtgaat atggcgtgta
     4921 tccaggtcgt tggccaggat gcggcggtct gcatggcctg cggagcaggg cagcttgaac
     4981 tgaacactca catgccggtc atcggcgcga accttgtgaa gatgcttcat ctgctgtccg
     5041 gtgccgcgcg gtcgctggcg gaccagtgcg tgtgcggcct tacggcaaac gagagggtct
     5101 gccgcaggaa ctttgaaaac agtgccagcc ttgcgacggt gctgaatccg gcgctcggat
     5161 atgaccgtgt ggccgaactg gtcaaggagt ccatatcctc gggccgtggc ctgaaggagc
     5221 tcgtgctggc aagggaactg ctggatgaag ccggctggga cgcgctcatg aaaaactgca
     5281 ccggtccact tccttgatgg cacagacgaa aaaacgattg atttttgttt tttgtgcgct
     5341 agtgtgtctg cgttccaatg acggtgggtg tagctcagtt ggttagagcg acaggttgtg
     5401 gccctgtagg tcgcgggttc gagacccgtc actcacccca ttataaaaca gcggcagagt
     5461 gaaaatttca ttctgccgct gttttttggc cgttttgtcc caaaaaaaac gaaaaaagtc
     5521 aaaaaatcgc ccaaaacagc caaaaaaacg caaaaaatgg gttcttcgtc aattatcgtg
     5581 gaaagagatc gagctttccg cagtgaaaaa gg
//
LOCUS       DAOQWR010000151         5580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_14823_length_5580_cov_5.360543, whole genome shotgun sequence.
ACCESSION   DAOQWR010000151 DAOQWR010000000
VERSION     DAOQWR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14823_length_5580_cov_5.360543"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKK36_10240"
     CDS             complement(<1..186)
                     /locus_tag="PKK36_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HNR94969.1"
                     /translation="MKELVVISGKGGTGKTSVTASFAVLAGRAVLADGDVDAADLHLL
                     LQPKIKHREEFRGGREAV"
     gene            complement(183..1040)
                     /locus_tag="PKK36_10245"
     CDS             complement(183..1040)
                     /locus_tag="PKK36_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR94970.1"
                     /translation="MNNSTYTVAVASGKGGTGKTTVAVNLAAVLAEQGTVVQYLDCDV
                     EEPNGHIFLKPEIMGSETVTMKVPVIDAARCTGCRKCAEACQFNAIAVPKKAMVFTEL
                     CHGCGACTLACPEDAITETDENIGAVESGKSGAILFGQGRLKVGISITPPIVRAVKRT
                     RKEGAVVILDSAPGTACPVVAAVRDVDYVILVTEPTPFGLNDLRLAVELMRELGRPFG
                     VVINRAGIGNDEVKKYCEAGNIALLAELPDDRRVAEAYSRGEMMITVPGFREILREAG
                     ARVMQEVVS"
     gene            complement(1033..1893)
                     /locus_tag="PKK36_10250"
     CDS             complement(1033..1893)
                     /locus_tag="PKK36_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNR94971.1"
                     /translation="MKTVIECIPCFARQAAEAVAQTVPDPVRREELLRSLLKAIAEEP
                     WDGTPPVMAQRIHRLIRQEVGSEDPYRGLKSRMNSAAETMVPLLRRRLEECQDRHEMA
                     VKLAIAGNLLDAGAKTQIGPDELEDHIEKILTAPLRGDPKILFRAAEEAHCILYLADN
                     AGEIFFDRLLIEELPAEKITLFVRGAPTINDATLADAERAGLPEILPVWGNGSDAPGT
                     IVEDCDEKFREWFECADLIISKGQGNYETLSDTDKHIFFLFTVKCPVVAEQTGEPVGS
                     MMVMERNGNE"
     gene            complement(1890..2273)
                     /locus_tag="PKK36_10255"
     CDS             complement(1890..2273)
                     /locus_tag="PKK36_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNR94972.1"
                     /translation="MNLIWKDKSMPDEPFLGRMLVPTASAFLKGPCGDEMEFYLKITD
                     GRIEDVTYYTNGCEHTRTCGAAAAEMAKGRTVTEALGISPREIIESRKCLPEEGKHCA
                     ILAVSTLYRAIANFLLSDEAWRARN"
     gene            complement(2270..2836)
                     /locus_tag="PKK36_10260"
     CDS             complement(2270..2836)
                     /locus_tag="PKK36_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR94973.1"
                     /translation="MLKKIHLCMNHARRVKRGRELSFAPGLNLLLGPNGTGKSTILRA
                     IAECEECRRIEEGPTEYVFFDAELANPHTATGPAGNYTNMILRTRALFSSHGEILQDV
                     FKTLGITPKTCLLLDEPEAGQDYDHVLKLRAAMDRAVKRGAQIICATHEFMFWERAHF
                     IELRRGYRKRISKAVCRMKCLNEQEDTL"
     gene            complement(2873..3391)
                     /gene="tsaA"
                     /locus_tag="PKK36_10265"
     CDS             complement(2873..3391)
                     /gene="tsaA"
                     /locus_tag="PKK36_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNR94974.1"
                     /translation="MKTAERTPFAYRTIGIIRTPHRDSTHTPYQPTTASSHEYECFIE
                     LAETYLPALARLEEFKYIYVIYHMDRLHRSVEMTVIPPWANRIQVGLFASRTPLRPNP
                     IGLSIVQLREIRSNRIYTSNLDVFDGTPLLDIKPYIRDLDSKTEANCGWIDDLPDRDH
                     LTLHIRGIPHDY"
     gene            complement(3388..4032)
                     /locus_tag="PKK36_10270"
     CDS             complement(3388..4032)
                     /locus_tag="PKK36_10270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR94975.1"
                     /translation="MPKTEPFDQLGDRYAAWFEKHPAAYRSELAAVRELWPDGANGLE
                     IGGGAGHFAGPLGIRRGVEPSEAMRLKALKRKIAAVAGVAENLPFPNACFDAVLMVTT
                     ICFVDDPQKSLQEMFRVLRPGGCAVIAFVDRDSPLGREYESKRAESPFYRDAHFFNAA
                     EVTALMKETGLVDLEYRQTLFTHPGSMRAEDPVRPGFGEGSFVVIRGRRPGAQS"
     gene            complement(4037..4387)
                     /locus_tag="PKK36_10275"
     CDS             complement(4037..4387)
                     /locus_tag="PKK36_10275"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNR94976.1"
                     /translation="MMRIMLKSKIHRAFITDTKLHYEGSIACDPCLLKAADILPNEKV
                     LVVNLNNGERFETYAIPGTDGEIGLRGAAARLGHAGDCVIIMAFGLMDSAECASWVPR
                     TVHVDRANRLIPEK"
     gene            complement(4384..5355)
                     /locus_tag="PKK36_10280"
     CDS             complement(4384..5355)
                     /locus_tag="PKK36_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR94977.1"
                     /translation="MMMQPYRYIFGPVPSRRFGRSLGVDLTPLKTCTLDCIFCQLGHT
                     SNKTIERREYVPVREVQQELIRWEKEGSEADFITLSGSGEPTLHSGFGEILKFIKDKM
                     PAPAVLLTNGTLLWMPEVRKAAANADIAKLSLSAWDEASFKRINQPHADITFERYVNG
                     LRSFRAEYSGKIWLEVFLIDGINSARGQVEKIARIANAIEADEIHLNTAVRPPADSAV
                     HPVPEERMAELADLFRPAATIIAGFPKRCAANIQANEETILNMLRRRPCTARQIAEVF
                     GMHVNEVSKYAGDLLRSQKIRFQYRDGEIYFAAVTDISNDEHEEVQS"
BASE COUNT         1112 a         1729 c         1509 g         1230 t
ORIGIN      
        1 aaccgcctcg cgcccgccgc gaaactcctc gcggtgctta attttcggct gcagcagcag
       61 gtgcaggtcg gcggcatcca catcgccgtc agccagcaca gcccttccgg ccagcacagc
      121 aaacgatgcc gtgacactgg ttttgcctgt tccgcccttg ccgctgatta ccacaagttc
      181 cttcacgaca ccacttcctg cataacccgc gcacccgctt cccggagaat ctcccggaat
      241 cccggcaccg tgatcatcat ctcgccgcgc gaataggcct cggccacacg gcggtcgtcc
      301 ggcagttcgg ccagcagcgc gatatttccc gcctcgcagt atttttttac ctcgtcgttg
      361 ccgatgcccg cgcggttgat cactacgccg aacggacgcc ccagttcgcg catcaactcg
      421 accgcaaggc gcagatcgtt caagccaaaa ggggtcggtt cggttacaag aataacataa
      481 tccacatcgc gaacggcggc taccaccggg caggccgtgc cgggcgcgga atccagaatc
      541 accactgcgc cttccttccg ggttcgcttg accgcccgaa cgatcggagg cgtaatggag
      601 attccgacct tgaggcgtcc ctgaccaaaa agaatggccc cggatttccc gctttccacg
      661 gctccgatgt tttcatcggt ttcggtgatc gcgtcttccg gacatgccag agtgcacgcg
      721 ccgcagccgt ggcagagctc ggtgaaaacc atcgcctttt tcggcaccgc aatggcgttg
      781 aactgacagg cctcggcgca tttccggcag ccggtgcagc gcgcggcatc aatcaccggc
      841 accttcatgg tgacggtttc ggatcccatt atttccggtt tcagaaatat gtgcccgttg
      901 ggttcttcga catcgcagtc cagatactgg acaaccgtgc cctgttccgc aaggaccgcg
      961 gccagattca cggcaaccgt cgtcttcccg gttccgccct tgccgctggc gaccgccact
     1021 gtgtaggttg aattattcat ttccattgcg ctccatcacc atcatgctgc ccaccggctc
     1081 acccgtctgc tccgcaacga ccgggcattt gactgtgaac aggaaaaata tgtgcttgtc
     1141 cgtatccgac agggtttcat agttgccctg cccctttgag ataatcaggt ccgcacactc
     1201 gaaccattcc cgaaactttt cgtcgcaatc ctctacaatt gttcccgggg cgtccgaccc
     1261 gttcccccac acgggcagga tttccgggag gccggcccgc tccgcatccg ccagcgtggc
     1321 gtcattgatc gtcggcgctc cgcgcacaaa cagggtgatt ttttcagcgg gcagttcttc
     1381 gatcagcagc cggtcaaaga agatctcgcc ggcattgtcc gccagataaa gaatgcagtg
     1441 cgcttcctcc gcggcacgga aaagtatttt cggatcgcct cgcagcggtg cggtcaagat
     1501 cttttcgata tggtcttcca gttcatcggg cccgatctgc gttttcgccc cggcgtcgag
     1561 caggttgccg gcaatagcca gcttgacggc catttcatga cggtcctgac attcttcaag
     1621 ccgccggcgc agaagcggca ccatggtttc cgccgcgctg ttcatacggg actttaaacc
     1681 gcgataggga tcttcgctgc ccacctcctg acggataagg cggtgaatac gctgggccat
     1741 gaccggaggg gtgccgtccc acggctcctc cgcaatggcc ttcagcaggc ttcgcagcag
     1801 ctcttcacgc cggaccggat ccggaaccgt ctgcgcgaca gcctcggccg cctgacgcgc
     1861 aaaacaggga atgcattcga tgactgtctt caattccgcg ctctccatgc ttcgtccgac
     1921 aacagaaaat tcgcaatggc ccgatataga gtgctgacgg caagaatcgc gcagtgtttg
     1981 ccctcttccg gaaggcactt tcgcgactca atgatttcac gcgggctgat gcccagcgct
     2041 tccgtcaccg ttctgccctt tgccatttcg gcggcggcgg ctccgcaggt tctcgtgtgc
     2101 tcgcatccgt ttgtataata ggtcacatct tcaatgcgcc cgtctgtgat tttgaggtag
     2161 aactccattt cgtctccgca cgggcccttt aaaaaagcag aggcggttgg aaccagcatc
     2221 cgcccgagga acggttcatc cggcattgat ttgtctttcc agatcagatt cacaatgtgt
     2281 cctcctgctc gttcaagcac ttcatgcggc agacggcctt gctgatgcgc ttgcggtagc
     2341 cccgcctcaa ttcaataaaa tgggcccgct cccagaacat aaactcgtgc gtggcgcata
     2401 tgatctgagc gccgcgcttc acggcgcgat ccatcgccgc ccgcagtttc agcacatgat
     2461 cataatcctg tccggcttcc ggttcgtcca gcagcaggca ggtcttgggc gtaataccga
     2521 gcgtcttaaa aacatcctgg agaatttctc catgcgacga aaaaagcgcg cgcgtccgga
     2581 ggatcatgtt cgtgtagttt ccagccgggc ctgtggcggt atgcggattg gcgagttcgg
     2641 cgtcgaagaa gacatattcc gtcgggccct cctcgatgcg gcggcactct tcgcattcag
     2701 cgatggcccg aagaatggtg cttttgcctg tgccgttggg ccccagaagc aggttgagcc
     2761 cgggtgcaaa agacagttct ctgccgcgct tgacccggcg cgcatggttc atgcacaaat
     2821 gaattttctt cagcatgaca acgccccctg tgtccgcctg tgtaaggaaa ttttaataat
     2881 catggggaat tccccggata tgaagggtta aatgatcgcg atccggcaaa tcgtcaatcc
     2941 agccgcagtt tgcctcggtt ttgctgtcga gatctctaat atagggcttg atgtccagca
     3001 ggggcgtgcc gtcaaacaca tccagatttg acgtgtatat gcggttggaa cggatctccc
     3061 gcagttgaac gatgctcaat ccaatcggat tggggcgcag aggcgtccgg ctggcaaata
     3121 aacccacctg aattctattc gcccaagggg ggatcacggt catctccacg ctccgatgaa
     3181 ggcggtccat atgataaatc acataaatat atttgaattc ttccaagcgc gccaaagcgg
     3241 gaagatatgt ttctgccagc tcaatgaagc actcatattc atggctgctc gccgtcgttg
     3301 gctgataggg cgtatgggtg gaatcacgat gcggagtacg aatgattcca atggtccgat
     3361 atgcgaacgg cgtcctctct gcggttttca tgattgtgcc cccggccgcc gcccgcgaat
     3421 cacaacgaac gatccttcgc cgaatccggg acgcaccgga tcttcagccc gcattgaacc
     3481 cgggtgtgtg aagagtgtct gacgatattc caaatccacc aagccagtct ccttcatcaa
     3541 ggccgtcacc tcggccgcat tgaagaaatg agcatcgcga tagaacgggc tttcggcgcg
     3601 tttggattcg tactcgcgcc ccagcgggct gtcgcggtca acaaacgcga ttaccgcgca
     3661 acctcccggc cgtagaacgc ggaacatttc ctgcaggctt ttttgaggat catccacaaa
     3721 gcaaatggtc gtcaccatca gaacggcgtc aaaacatgcg ttcggaaacg gaagattttc
     3781 ggcgacaccg gcaacggcgg cgatctttct tttcaacgcc ttcagccgca tcgcttccga
     3841 aggctccacc ccgcggcgga tgcccagggg cccggcaaaa tgcccggcgc cgccgccgat
     3901 ctccagcccg tttgcgccgt ccggccacag ctcgcgcacg gcagccagct ctgaacgata
     3961 ggctgcggga tgtttctcaa accacgccgc gtaccggtca ccaagctgat caaacggttc
     4021 agttttcggc atcttgctat ttctccggaa tcaggcggtt ggcgcggtcc acatgcaccg
     4081 tgcgaggcac ccatgatgcg cattcggcgc tgtccatcag gccaaaggcc atgatgatca
     4141 cgcagtcccc cgcatgcccc agccgcgcgg ccgcgccgcg caggccaatc tcgccgtccg
     4201 tgccgggaat cgcataggtc tcgaagcgtt ccccgttgtt caaattaacc accagcacct
     4261 tctcgttcgg gaggatgtcg gcggccttca gcaggcatgg gtcgcaggca atgcttccct
     4321 cgtaatgcag ctttgtatca gtaataaaag cccggtgaat cttgctctta agcataattc
     4381 tcatcatgac tgcacctcct cgtgttcatc attgctgatg tcggttacag cggcaaaata
     4441 tatctccccg tcccggtact gaaaccggat tttctgcgaa cgcagcaggt caccggcata
     4501 cttggagacc tcgttcacgt gcattccgaa cacctcggca atctgccggg ctgtgcaggg
     4561 cctgcggcga agcatgttca aaatggtttc ctcgttggcc tgtatgttgg ccgcgcatcg
     4621 cttcgggaag ccggcgataa tggttgccgc cggcctgaac agatccgcaa gttcggccat
     4681 ccgctcttca ggcaccggat gcaccgcgga atcggccggc ggccggaccg ccgtgtttaa
     4741 atgaatttca tccgcctcga ttgcattggc aatccgcgca attttttcaa cctgcccgcg
     4801 ggcagaattg atgccgtcga tcaaaaatac ctccagccag attttcccgg agtattcggc
     4861 gcgaaaagac cgcagcccgt ttacatagcg ctcaaaagta atatccgcat gaggctggtt
     4921 gatgcgcttg aaggaagctt cgtcccatgc gctgagcgac agcttcgcga tatccgcgtt
     4981 tgccgcagct ttgcggactt cgggcatcca gagaagagtg ccgtttgtga gaagcactgc
     5041 aggcgcgggc atcttgtcct ttatgaattt gagaatctcg ccgaatccgg agtggagcgt
     5101 gggctccccg gacccggaaa gggtgatgaa atcggcttcg ctgccctcct tctcccagcg
     5161 gatcagttcc tgctggacct cgcgaacagg cacatattcc ctgcgttcaa tagttttgtt
     5221 ggatgtgtgc cccagctggc agaaaatgca gtccagagtg caggttttaa gcggggtgag
     5281 atccacccca agcgagcgcc cgaagcgacg cgatggcact ggaccaaaaa tgtaacggta
     5341 aggctgcatc atcattcggg ttatgcggcg cgcgggattt tccgcgcgcc gcagcattcc
     5401 tgttctattt cttctcggtc ttttccagtt cctgaatgcg gctgcggatt tcctcgagcg
     5461 cctgcccgaa atattccgcc tgctgcttca ggcccgcgag ttcctgttca ggcgcggccg
     5521 ccagcggcac cgaagcgccc caggggccgg ccatccagcc cgggactccc gtggcgtaaa
//
LOCUS       DAOQWR010000152         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15084_length_5501_cov_4.898825, whole genome shotgun sequence.
ACCESSION   DAOQWR010000152 DAOQWR010000000
VERSION     DAOQWR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15084_length_5501_cov_4.898825"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKK36_10285"
     CDS             <1..187
                     /locus_tag="PKK36_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94978.1"
                     /translation="GHSGHDVLLVVPSLDLVVCWVDAFPKKTHATRFSEGRKNVNKAA
                     ALLMAALQPAAEGDLQR"
     gene            184..888
                     /locus_tag="PKK36_10290"
     CDS             184..888
                     /locus_tag="PKK36_10290"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNR94979.1"
                     /translation="MKVQPGRLRQQIMLSEESSVFVLGSWMLIAWIAAITILWRMGHP
                     SWMQWIIMGFTELFLGRGVAIAKAVEMGMHPGLIIFLATYVDAVTVFIFYPALILAYR
                     NLLEGRFVDERMKGIIESAEKNVGRFAKYKIAGVFIFVWFPFFMTGVVVGAVLGYLLG
                     LKTWTNMITVTVGTMSAAVCWMYAYDNLYGWLETIHPEIPLFFTVILVAGLAAGRLWM
                     EAKKKRDLRKADQPHA"
     gene            complement(873..1298)
                     /locus_tag="PKK36_10295"
     CDS             complement(873..1298)
                     /locus_tag="PKK36_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNR94980.1"
                     /translation="MATRITIELPEPLPFTTRISVRIGDINYGGHVGNDAMLRIMQDA
                     RLRFLAQYGWSELDTAGASLIMTDACVIYKAQARFGDDLEIGVASRDLTRTGFTLVYR
                     IVRTQDRVEIARGETGMAFFDYGRNRVARMPEAFRQACG"
     gene            complement(1300..1797)
                     /locus_tag="PKK36_10300"
     CDS             complement(1300..1797)
                     /locus_tag="PKK36_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94981.1"
                     /translation="MQRFIHITLAWACILGAGSAQDIFLDVGRPEAREHLGYGWGGNE
                     RSGPTRFAWIKELEGDVWFDLMAAENLEITIRAVPFFVEHRRQPVALYVNNRYVTEWV
                     CPHRSEWNFNPYTAAVPAELLKPGRNRLTLRMGYHVRSKKRFHALAVESIRIRPVESE
                     AEEGI"
     gene            complement(1841..3370)
                     /gene="cls"
                     /locus_tag="PKK36_10305"
     CDS             complement(1841..3370)
                     /gene="cls"
                     /locus_tag="PKK36_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNR94982.1"
                     /translation="MNWTSFTDRLPGADIQAPWWEWLSLGGVLHLVFFLLVLMHCLRR
                     RREAPATLLWIFVAWAFPFIGPLVYLAFGIDRVPQKVFSKYRKDQALWKELKARNAET
                     LPLAYWRAVHEAVADIPRGAPGHRFNRAVDSMLSHHPLLDGNRIAPLVNGDETYPEMI
                     KAIRSAGHHIHLQSFIIGSDATGRMFMDLLAEKAREGVTVRLLYDRFGCTKAILSGFF
                     RKYRRIPNFHMAGWTLANPLRRQFQVNLRNHRKCLIVDGRLAFTGGINLSDDNITSAE
                     RPAIRDYHFEVAGPIVQELQYTFLRDWYFITDEDPDHFLQEVYFPQLPSIGTARVRQV
                     DSGPSHVSEVLTDVYFAAIVSARNDLLIVTPYFIPNRDLVRALRAAALRGVNVRLIVP
                     QKNNHFYAGLAGRALYEELLEAGVRIFERKPPFLHAKALIVDDSAALVGTANFDVRSF
                     RLNYESTLAVFDTDFCNRLKPIILCDLAESGEIMLSEWAKRPARRKMLENLAFLMMPA
                     L"
     gene            3453..4190
                     /locus_tag="PKK36_10310"
     CDS             3453..4190
                     /locus_tag="PKK36_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNR94983.1"
                     /translation="MKMRFVLYNMCYATGGRSRIFPFMNYLRHTHAKLEEIISFFRAL
                     NPDIIGLVEVDAGSYRSSRANQAELIASRLGHYHAYRSKYHDAHLASRLPLMNKQGNA
                     FVTRDSFAELKFHYFTRGIKRLVIELEMENLTIFLVHLALGFKIRHHQLEHLYQLVRN
                     TGKPHIVAGDFNAFWGDVEISLFLAATGLVRAGPPDLPTHPSWAPKRSLDFILHSPRI
                     RPTCFWAPHVTLSDHLPLVCDFEVLPE"
     gene            4275..4841
                     /gene="yeiP"
                     /locus_tag="PKK36_10315"
     CDS             4275..4841
                     /gene="yeiP"
                     /locus_tag="PKK36_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HNR94984.1"
                     /translation="MIKACSITKNSIVEIGGAPHTVENIVVQTPSARGGATLYKIRFR
                     NIKTRQKVDQTLRGDDPLKEADFETRNVQYLYKNGDVFTFMDTEDYSQFELNEEEIED
                     AIPYLVEDMEGITALFSDGRVLGIRMPDVVEMKIEECDPSIKGASATARSKPAKLTTG
                     LTVLVPEYISPGEVIRVDTRTNSFLSRA"
     gene            4844..>5501
                     /locus_tag="PKK36_10320"
     CDS             4844..>5501
                     /locus_tag="PKK36_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR94985.1"
                     /translation="MSKRSVEYIYGINPIFEVIRAGRRSIKGAFLSLSSANNPRIKKL
                     EEYLLRKQIPVEWVEKGRVMDLSGSKDNQGAVIRSSTYPYVPFEEVIAAQQVLLLDNV
                     EDPHNVGAILRTAEIFGFSHVLAPMKGVPDIYPSVVKVSAGATEFLKICKDSSANIYA
                     RKAHEAGFTIIALDGAGKTALEDVNVSSGDRVMLVIGGEDKSVGQYILNMADYVVRIE
                     Q"
BASE COUNT         1126 a         1585 c         1654 g         1136 t
ORIGIN      
        1 tgggcactca gggcatgatg tgctgctcgt cgtgccttca ctggacctgg tggtgtgctg
       61 ggttgacgcg ttcccgaaaa agacccatgc gacgcggttt tctgaaggcc ggaaaaatgt
      121 caacaaggca gccgccctgc tgatggcggc gctgcagcct gccgcggaag gggatctcca
      181 gcgatgaagg tgcagcccgg cagactgcgg cagcagatca tgctttccga ggagagcagc
      241 gtgtttgtgc tgggcagctg gatgctcatc gcgtggatcg ccgccatcac gattctgtgg
      301 cgcatggggc atccttcatg gatgcagtgg atcatcatgg gttttaccga gctctttctc
      361 gggcgcggcg tcgccatcgc caaggctgtc gaaatgggaa tgcatccggg actcattatc
      421 tttctcgcaa cctatgtgga tgccgtcacc gtgttcattt tttatccggc gctgattctg
      481 gcctatcgca atctgctgga gggccggttt gttgatgaac ggatgaaggg catcatcgag
      541 tcggccgaga agaacgtcgg gcggtttgca aagtacaaga tcgcgggggt cttcattttt
      601 gtctggttcc cgtttttcat gacgggggtg gtggtcggcg cggtgctggg ttatctgctg
      661 ggcctgaaaa cctggaccaa tatgatcact gtgacggttg gaaccatgtc tgcggccgtc
      721 tgctggatgt atgcgtatga caatctgtac ggatggctgg agactattca tccggagatt
      781 ccgctgttct tcactgtaat cctggtcgcc gggctggccg ccggccggct ctggatggag
      841 gcgaaaaaga agcgcgatct gcgcaaggca gatcagccgc atgcctgacg gaaggcctcc
      901 ggcatgcgcg ccacgcggtt gcggccgtaa tcgaagaacg ccatccccgt ttcaccccgg
      961 gcgatctcca cacggtcctg cgttcggact atgcggtaaa ccagtgtgaa tcccgtgcgc
     1021 gtcagatccc gcgaggcaac accgatttcc agatcgtccc cgaaccgcgc ctgggcttta
     1081 tagatcacgc aggcatccgt catgatcagg ctggcgccgg ccgtgtccag ttcgctccag
     1141 ccgtactgcg ccagaaaccg cagccgcgca tcctgcatga tccggagcat ggcgtcgttg
     1201 ccgacatgtc cgccgtaatt gatgtccccg atgcggacgg aaatgcgcgt ggtgaagggc
     1261 agcggttccg gaagctcaat tgtgattctt gtggccatgt cagatgcctt cctctgcttc
     1321 ggattccacg ggccggatcc gtatggattc gacggccagc gcatgaaaac gcttcttgct
     1381 ccggacgtga taacccatgc gcagcgtcag ccggttgcgc cccggcttca gcagttccgc
     1441 cgggacggct gcggtgtacg gattgaagtt ccattcgctt ctgtgggggc agacccattc
     1501 cgtcacatag cggttgttga catacagggc cacgggctgc cggcggtgct cgacaaagaa
     1561 gggaaccgcg cggatggtga tttcgaggtt ttccgccgcc atcaggtcaa accagacatc
     1621 cccttcaagc tccttgatcc atgcgaagcg ggtcgggccc gagcgttcgt tcccgcccca
     1681 gccgtatcca agatgctcgc gggcttcagg ccggccgaca tccaggaaga tgtcctgcgc
     1741 gctcccggct ccgaggatgc aggcccatgc gagggttatg tgaataaatc tctgcatagg
     1801 cgtcatcatc cagattttgc gccgtgaaac aagcaaaacc ctacagcgcg ggcatcatca
     1861 ggaaggcgag attttcaagc attttccggc gggccgggcg ctttgcccat tcgctcagca
     1921 tgatttcacc gctttcggcc aggtcgcaga gtatgatcgg cttcaggcga ttgcagaaat
     1981 cggtgtcaaa gaccgcaagg gtggactcgt agttcaggcg gaagctgcgc acatcaaaat
     2041 tggccgtgcc gaccagcgcg gcggaatcat ccacgatcag cgccttggcg tgcaggaagg
     2101 gcggcttgcg ctcgaatatc cttactcccg cctccagaag ttcttcatac agggcccgtc
     2161 cggccagccc ggcatagaaa tgattgtttt tctgcggaac aatcagccgg acattgacgc
     2221 cgcgcagcgc ggctgcccgg agcgcccgga ccaggtcgcg gttgggaatg aaataggggg
     2281 tcacgatgag caggtcgttt cgcgccgaaa caatggccgc gaaatagaca tccgtgagaa
     2341 cttcggaaac atgggagggg ccgctgtcca cctgccgcac ccgcgccgtg ccgatggacg
     2401 gaagctgggg gaaatacacc tcctgaagaa agtggtccgg gtcctcatcc gtgatgaaat
     2461 accagtcgcg gagaaaagtg tactgcagct cctgaacaat gggcccggcc acttcaaaat
     2521 ggtagtcgcg gatcgcgggg cgctccgcgc tcgtgatgtt gtcgtcggac agattgatgc
     2581 cgccggtgaa ggccaggcgc ccatccacga tgaggcattt gcggtgattc cgcagattga
     2641 cctggaactg gcgtctcagc ggattggcca gggtccagcc ggccatgtga aaattaggaa
     2701 tccggcggta tttccggaag aagcctgaaa ggatggcttt ggtgcatccg aaccggtcat
     2761 aaagcagccg cacggtgacc ccttcgcgcg ccttttccgc cagcaggtcc atgaacatgc
     2821 gtcccgtcgc atcgctgcca atgatgaagc tctgcagatg gatgtggtgc ccggctgaac
     2881 ggatggcctt gatcatctcg gggtaggtct cgtcgccgtt cacgagggga gcgatcctgt
     2941 ttccgtccag cagggggtga tgcgaaagca tggaatcgac cgcacggttg aaccggtgcc
     3001 ccggcgcgcc ccgcggaatg tcggccaccg cttcatgcac ggcccgccag tatgcgaggg
     3061 gcagggtttc cgcattgcgc gccttcagtt ccttccacag ggcctgatcc ttgcggtatt
     3121 tgctgaagac cttctgcgga acccggtcta tgccgaaggc cagatagacc agcgggccga
     3181 tgaacgggaa ggcccaggcg acgaatatcc agagcagggt cgccggagct tcgcgccgcc
     3241 gccgcaggca gtgcatcagc accagcagaa agaaaaccag gtgaagcaca ccgcccagag
     3301 aaagccattc ccaccaggga gcctgtatat cggccccggg aaggcgatcc gtgaatgatg
     3361 tccagttcat gcggttattc taacggctgt tttctgctta ttcaattcct attgcttcct
     3421 gcttcatttt tggaggcctg cgcggtaaaa taatgaaaat gaggtttgta ctctacaaca
     3481 tgtgctatgc gaccggcggc cggagccgga ttttcccgtt catgaattac ctgcggcata
     3541 cgcatgccaa gctggaggaa atcatctctt ttttccgcgc gctgaatcct gacatcatag
     3601 gccttgtgga ggtggacgcc ggatcctacc gctcatcgcg ggcgaaccag gcagagctca
     3661 tcgcctcccg tctcggacat tatcacgcct atcgctcaaa atatcacgat gcccatctgg
     3721 ccagccgcct tccgctgatg aacaagcagg ggaacgcatt cgtcacgcga gattcctttg
     3781 ccgaactcaa gtttcattat ttcacccgcg gcataaagag gctggtcatc gaactggaga
     3841 tggaaaatct gacgatattt ctggtgcatc tggcgctggg cttcaagatc cgccatcatc
     3901 agctggagca cctgtaccag ctcgtgcgaa acaccggcaa accccatatt gtggccggtg
     3961 acttcaatgc cttctggggc gatgtcgaga tcagcctgtt tctggccgcg accggcctgg
     4021 tccgcgccgg gccgccggac ctgcccacgc atcccagctg ggccccgaag cggtccctgg
     4081 acttcattct gcacagcccg cgcatccgcc cgacctgctt ctgggcgccg catgtcacgc
     4141 tctcggacca tttgccgctc gtgtgcgatt tcgaggtgct gcctgaatga aaatgcgcca
     4201 ccggcaaagc ttcgcattgc gcgcgggacg accatgtttt acactcccgg tccgttgaat
     4261 caggagaaga tattgtgatc aaggcgtgca gcataacgaa aaattcaatt gtggaaattg
     4321 gcggagcccc tcacaccgtg gagaacatcg tggtgcagac cccgtccgcc cgcggcgggg
     4381 ccactctgta caaaatccgt ttccggaaca ttaaaacccg gcagaaagtg gaccagaccc
     4441 tgcgcggcga tgacccgtta aaggaggccg actttgagac gcggaatgtc cagtacctct
     4501 acaagaacgg cgatgtcttt accttcatgg acacagagga ctattcgcag ttcgaactga
     4561 atgaggagga gattgaagac gccatcccgt atcttgtgga ggacatggag ggcatcaccg
     4621 cgctgttcag cgacggccgt gtgctgggga ttcgcatgcc ggacgttgtg gaaatgaaaa
     4681 tcgaggagtg cgacccgtcc atcaagggcg cctccgccac ggccaggagc aagccggcca
     4741 aactgaccac cggcctgact gtgctcgtgc cggagtatat ctcgccgggc gaggtgatcc
     4801 gcgtggacac ccgcaccaac tcgttccttt cgcgggcctg atcatgagca aacgctccgt
     4861 tgaatatatt tacggaatca accccatttt cgaggtgatc cgtgccggcc gccgcagcat
     4921 caagggggcc ttcctgagcc tctcttcggc gaacaatccc cggatcaaaa agctggagga
     4981 atatctgctc cggaaacaga tcccggtgga atgggtggag aagggacgcg tgatggacct
     5041 gtccggcagc aaggacaacc agggcgcggt gatccgttcc agcacctatc cctatgtgcc
     5101 ctttgaagaa gtgatcgccg cacagcaggt gcttctgctc gacaacgtcg aggacccgca
     5161 caacgttggc gccatcctcc gcacggcgga gatttttgga ttcagtcatg tgctggcccc
     5221 catgaaaggc gtccctgaca tttatccctc ggtggtcaag gtgtcggccg gcgcaaccga
     5281 attcctgaag atatgcaaag actcatccgc aaacatctat gcccgcaagg cgcatgaggc
     5341 gggcttcacc atcatcgcgc ttgatggagc cggtaaaacg gcgctggaag acgtgaatgt
     5401 ttcgtccggc gatcgtgtga tgctcgtgat cggaggcgaa gataagtccg tcgggcagta
     5461 tattctgaac atggccgatt atgtggtccg catcgagcag a
//
LOCUS       DAOQWR010000153         5381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15528_length_5381_cov_5.390725, whole genome shotgun sequence.
ACCESSION   DAOQWR010000153 DAOQWR010000000
VERSION     DAOQWR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15528_length_5381_cov_5.390725"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1040
                     /locus_tag="PKK36_10325"
     CDS             192..1040
                     /locus_tag="PKK36_10325"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94986.1"
                     /translation="MRLLCFIIASGLVLLMAPAGYSQGALETPLHINARDVILDEFGE
                     VLRGSATAPGDLVQVLRAEDGVIFPPAVDGRPHSSNPLMDRGTAGIGKFMPLSQTRPG
                     KFGLSLGGLARPVESTKLFIRVFNAPTLAEASFYADSELLTVKGNSVLMAGIGRTTNA
                     LDKADDDGDGLNNSWEKSYESDPGNADTDGDGMLDGDEHLAGTSPTDEGSEFVVARIS
                     RGRLSWKSALGRRYQIQRADAAGGEYVNIGDVVDADPVLDETEIDIPDGAGSGMGFYR
                     VKLVIE"
     gene            1275..1601
                     /locus_tag="PKK36_10330"
     CDS             1275..1601
                     /locus_tag="PKK36_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR94987.1"
                     /translation="MVRDEKRMVAGISPVLRAQITEIICSQKPVNRIILFGSRARGKA
                     RRTSDIDLAVDGNGWTDQDVNLVHNRLEEEVPTALKFDLVNVSDISRPELKKEIFENG
                     KTIYER"
     gene            1591..1986
                     /locus_tag="PKK36_10335"
     CDS             1591..1986
                     /locus_tag="PKK36_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNR94988.1"
                     /translation="MSADRVKMWIAEYDRALARLQEAANADPAENDVVVDGTIQRFEF
                     TFELAWKLMKAALEHQGIVVQSPRAAIKEAFKVGLIADGDGWIDMLEDRNRTTHLYDE
                     EQCRQVYGKIKERHIGLMESLGREVGKTE"
     gene            2178..2672
                     /locus_tag="PKK36_10340"
     CDS             2178..2672
                     /locus_tag="PKK36_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNR94989.1"
                     /translation="MAEKPTVYIETTIPSFLTARPSGDLIAAGEQEVTRQWWEKRREA
                     FQLFVSQLVLTEAAKEDSAAARRRLDVLNDIPVLAADDEADSLLEVIRVSGIMPPKAS
                     ADAAHVAVAARHEMDFLMTWNCRHIANAELIPRLTAIVSGAGYAMPVICTPNELMGGL
                     GDDA"
     gene            2713..2877
                     /locus_tag="PKK36_10345"
     CDS             2713..2877
                     /locus_tag="PKK36_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94990.1"
                     /translation="MDSYGGDVHKMFRDVMKRQWESGHKVVSFPLKREKTAARVAEGQ
                     AEYKAGSGKN"
     gene            3125..3589
                     /locus_tag="PKK36_10350"
     CDS             3125..3589
                     /locus_tag="PKK36_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNR94991.1"
                     /translation="MKVLRIYLDTSVFGGCFDEEFAKESRRLIEEIKAGRFRLLLSEA
                     VVAEVERAPVDVQKVLAGIPEDCIEVLPPDPAVKILMRAYLDAQIVGPASATDAEHIA
                     SATVARADMIVSWNFKHIVHYEKIEGYLSVNRQLRYGEIGIYSPKEVVDYEN"
     gene            3579..3815
                     /locus_tag="PKK36_10355"
     CDS             3579..3815
                     /locus_tag="PKK36_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR94992.1"
                     /translation="MKTKTFDCVKMKEECQQAVLKKYGGLPPGDRERQMEADILASPI
                     LGDFYRRIFLAEAGKVRSRVAEGQAEYKAESGKN"
     gene            4021..>5381
                     /locus_tag="PKK36_10360"
     CDS             4021..>5381
                     /locus_tag="PKK36_10360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNR94993.1"
                     /translation="MSKTDKKDRNEAGRNFRGMPWMLLALALLAFIPYLPALRADFLD
                     WDDPYYVTHNFRIHQFSPATVADIFRLEPQLGPIPNGQYTPLTELTLMIEHRLVGLSP
                     WLFHLNNLLIHVANVFLVYLLMRKLRADHLWAWLTAVLFAVHPLHVESVAWISERKDV
                     LCALFYLITMVLYLHFRDTQRIRYFAAAVFSGLLALLSKPMAVTLPAVLVLCDLYLKR
                     PLSEQWKKPWMIPFAGLSLLFVAITLRTHTGYGIIRGGEVGNIFQNVMMACWNFFWFV
                     GKTVWPASLSAYVPVPSTTPATFAAYLLSLFGVMALVTGLGFYSWRRGFRLLPFAVFF
                     FIAALLPVSRLVPIGIRYMVADRFFYIPGIGFLMLMAYGLVHWIRSPGVLRALRVTVV
                     ALLVLFWGVLSFAQSRVWQTSETLWAHVLKRVPDASVALNGLALASLDEGRLDQSETF
                     LEQS"
BASE COUNT         1231 a         1266 c         1691 g         1193 t
ORIGIN      
        1 gaatgggtag agaaagctca ctcatttttt cctgaatctt gatgaataat tcaggctata
       61 catgatcccc ctttggggta actttttctt aagaaaacca ttgttaaaaa tttttaaccg
      121 gttatatttt aacatccggt gaaattttat ttttgcagag aaggtgacgt agaacggaga
      181 gggttacatt aatgagatta ttgtgcttca taatcgcttc agggctggtt ctcctgatgg
      241 cgcctgcggg ctattcccag ggggcgctgg agacacccct tcacattaat gcccgggatg
      301 tgatcctcga tgaattcggg gaagtcctcc ggggcagcgc caccgctccc ggagacctcg
      361 tgcaggtgct tcgcgccgaa gacggcgtca tcttcccgcc tgccgtggac ggccggccgc
      421 attcctccaa tccgctgatg gaccggggca cggccggcat cggcaagttc atgcccctca
      481 gccagacgcg tcccggaaag ttcggactat ccctcggcgg cctggcgcgc ccggtcgaaa
      541 gcaccaaact cttcattcgc gtattcaacg cgcccacgct ggcagaggcc tcgttctacg
      601 ccgattccga actgctgacg gtcaagggca acagcgttct gatggccggg atcggccgca
      661 ccaccaatgc gctggacaaa gccgacgatg acggcgacgg cctcaacaac tcgtgggaga
      721 agagctatga atccgatccc ggcaatgccg acacggacgg cgacggcatg ctggacggcg
      781 acgagcatct tgccggaacc agtccgacgg atgaaggatc ggagttcgtt gtggcgcgca
      841 tttcacgggg caggctctcg tggaaatcgg ccctgggccg gcgctatcag atccagcggg
      901 cggacgccgc cggcggggaa tatgtgaaca tcggcgacgt ggtggatgcc gatccggtgc
      961 tggatgaaac cgaaattgat attccggacg gagccggcag cggcatgggc ttctatcgcg
     1021 tcaagctggt gattgaataa tgggggagca ttcagcgcgt cacagggggg cgcacaatta
     1081 aacaaaaacc gcgaacagac gcgaatgaaa tttgaatagg aagaattctc gcgcagggac
     1141 gcggagacgc ggagaaatgc agcagccgct ctgcgagcgg cagttgggaa atctgaataa
     1201 ggaatgacgg aatggaggga cgtgcgatgc ctgcccgcca tcgctttgcg aaggcgggtc
     1261 acgtccggta aaacatggta agggatgaaa agagaatggt ggctggaatc agtccggttt
     1321 tgcgggcgca gatcaccgaa atcatctgtt cgcagaagcc tgtgaaccgc atcattctgt
     1381 ttggctcccg cgcgcgcggc aaggcccgcc ggacatcgga tattgatctg gctgtggatg
     1441 gtaatggctg gacggaccag gatgtaaacc ttgtccataa ccggctggaa gaggaagtcc
     1501 cgaccgcgct gaagtttgat ttggtgaatg tatcggacat aagccgtccg gaactgaaaa
     1561 aagagatctt tgaaaacggc aaaacgattt atgagcgctg accgcgtcaa gatgtggatt
     1621 gcggagtatg accgcgccct ggcgcgattg caggaggcgg caaatgctga tcctgcagaa
     1681 aatgatgtcg ttgtggacgg cacgatccag cgcttcgagt tcacttttga gctggcgtgg
     1741 aagctgatga aggcggcgct ggagcatcag gggatagtgg ttcagtcgcc gagggccgcg
     1801 atcaaggaag ccttcaaagt cggactcatc gcggatggcg atggatggat tgacatgctg
     1861 gaagaccgga accggaccac gcacctgtat gacgaagaac agtgccggca ggtgtatggg
     1921 aagattaaag agcggcatat tggcttaatg gagagtttgg gccgggaagt cggaaaaact
     1981 gaataggaag aattctcgcg cagggacgca aaggcgcggt gaaacgcgaa gaaatttgaa
     2041 taaagaagag ctgacaccgg atcaacagga tgaacaggat ggttatccgg taaatccagt
     2101 taatcctgtc aaaatgaatt tcgaagagaa tggctgaaaa agtgagaacg gaaacgagaa
     2161 tggtggaata gccgaaaatg gcggaaaaac cgacagtcta tattgagacc acgataccga
     2221 gctttttgac agcccggcca tcgggggatc tcattgcggc tggagaacaa gaggttacgc
     2281 ggcagtggtg ggaaaagagg cgtgaggcct ttcagttgtt tgtctcacag cttgttttga
     2341 cagaagccgc caaggaagac agcgccgcgg cccggagacg gctggatgtc ttgaatgaca
     2401 ttccagttct ggccgccgat gatgaagcag acagcttgct tgaagtcatc agggtctcgg
     2461 gcattatgcc gcccaaggcg tcggcagatg cggcgcatgt agccgttgct gcgcgtcatg
     2521 agatggattt tctgatgacc tggaactgca ggcatattgc caatgcagag ctgataccgc
     2581 gtttgactgc gattgtcagc ggggcgggtt atgcgatgcc ggttatctgc acgccaaatg
     2641 agctgatggg agggcttggc gatgacgcat gatgagatag ttggcgaggt tagaagagcc
     2701 agacaggaga ttttggattc ctatggcggc gatgtgcaca agatgtttcg agatgtcatg
     2761 aagcgccagt gggaatccgg gcacaaggtg gtgtcgtttc ctcttaagag ggagaagacc
     2821 gcggccaggg tcgcggaagg ccaggcggaa tacaaggcgg gaagcgggaa gaattgaata
     2881 ggaaaagttc gcgcgcaggg acgcggagat gcggagaaat acagcagccg ctctgcgagc
     2941 ggcaaaatga agatgcgcct cacagaggcg ctactacaaa gggattttga tgtagcagcc
     3001 gctctgcgag cggcagatga aaaactgaat aaaaaagttt tggagtgcgg cggctggacg
     3061 ccgctttgat gatcggaaga gaaagattag agagaagaac ggcaagaagc tgctgaacgg
     3121 tgcgatgaag gttttgagaa tatatctgga tacgtcggtt ttcggcggat gcttcgacga
     3181 agagttcgca aaggaaagcc gtcgcctgat cgaggagatc aaggctggcc ggttccgtct
     3241 gctgctttcc gaggcggtcg tggcggaagt ggagcgcgct ccggttgatg tccaaaaggt
     3301 gcttgccggt atccccgaag attgcattga agtgctgccg cccgatccgg ctgtgaagat
     3361 cctgatgcgc gcctatctgg acgcccaaat cgtaggaccg gcatccgcga ccgatgcaga
     3421 gcatatcgct agcgccacgg tcgcccgggc tgacatgatc gtgagctgga atttcaagca
     3481 cattgtccac tacgagaaaa tcgaaggcta tctatccgtc aaccggcagt tgcggtatgg
     3541 agagatcggc atttattccc ccaaggaagt ggtggactat gaaaactaag acttttgatt
     3601 gcgtgaagat gaaagaagag tgccagcagg ccgtcctgaa gaaatatggc gggttgccgc
     3661 ccggtgaccg ggaaagacag atggaggccg atatcctggc cagtcccatt ctgggagatt
     3721 tctatcgtcg gatatttttg gcagaagcgg gcaaagttcg ttcccgggtg gcggaaggac
     3781 aggcagagta caaggcagaa agcgggaaga attgaatagg aaaagttcgc gcgcagggac
     3841 gcggagaaat accgcagccg ctctgtgagc ggcaaaatga agatgcgcct cacagaggcg
     3901 ctactacaac gggattttga tgtagcggcc gctctgtgag cggcagatga aaaactgaat
     3961 aaaaaagatt tggagtgcgg cggcttgacg ccgctttgac agacgggaat ggaagtgcaa
     4021 ttgagcaaga cagacaagaa agaccggaat gaggcagggc ggaacttccg cgggatgccg
     4081 tggatgctcc ttgccctggc cctgctcgca ttcatcccct atctgccggc gctcagggcg
     4141 gatttccttg attgggatga tccctattac gtgacccata attttcgcat acatcaattt
     4201 tctccggcga ccgtagcgga catcttccgg ttggagccgc aactgggtcc cattccgaac
     4261 gggcagtaca ctccgctgac cgaattgact ttgatgattg agcataggct ggtgggactg
     4321 agtccctggc tgtttcatct caataatctg ctgattcatg tggcgaatgt tttcctcgtc
     4381 tatctgctga tgcggaagtt gcgtgcggac catctatggg catggctgac agcggtattg
     4441 tttgctgttc atccgctgca tgttgaatcg gtggcgtgga tatccgagcg caaggatgtc
     4501 ttgtgtgccc tgttctatct gattacgatg gttctatatc tgcatttccg ggatacacag
     4561 agaatccggt attttgcagc cgcggttttt tccgggcttc tggcgctttt gtcgaaaccg
     4621 atggcggtaa cgctgcccgc ggtgctggta ctgtgcgatc tttatttgaa acggcccctg
     4681 tctgagcagt ggaagaagcc atggatgatc ccgtttgcgg gactgagcct gttgtttgtc
     4741 gcaatcaccc tgcgtacgca taccggctat ggcatcatcc gtggaggaga ggtgggcaat
     4801 atctttcaga atgtgatgat ggcctgctgg aatttcttct ggttcgttgg gaaaactgtt
     4861 tggcccgcca gtttgagcgc ctacgtgccg gttccttcaa caaccccggc gacttttgcg
     4921 gcctatcttc tgtccttgtt cggagtgatg gcgctggtga ctggcctggg cttttattca
     4981 tggcggcgcg gtttccgtct gctgcctttt gccgttttct ttttcatcgc agctctgctt
     5041 ccggtgtcgc ggctggtgcc gattggaatc cgctacatgg tagccgaccg ctttttctat
     5101 attcccggca ttggattcct gatgctgatg gcctatggat tggttcactg gattcgcagc
     5161 ccgggtgttc tgcgggctct gcgggtgacg gttgtggcgc tgctcgttct tttctgggga
     5221 gtcctttctt tcgcacaaag ccgggtctgg cagacgagcg agacgctgtg ggcccatgtg
     5281 ctgaaacggg tgccggatgc ttccgtggcc ttgaatggtc tggctctggc ttctttggat
     5341 gagggtcgtc tcgaccagtc ggagacgttt cttgaacaat c
//
LOCUS       DAOQWR010000154         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15634_length_5354_cov_5.890545, whole genome shotgun sequence.
ACCESSION   DAOQWR010000154 DAOQWR010000000
VERSION     DAOQWR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15634_length_5354_cov_5.890545"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..652
                     /locus_tag="PKK36_10365"
     CDS             137..652
                     /locus_tag="PKK36_10365"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNR94994.1"
                     /translation="MKLKQIPRTPFGPVSLIWGLLNRVPLVSRVILPRPGRINEVNIP
                     DGSCAEIDAVAESISAILQGENCVIPIELADFSSCSDFQQKILRAQYQVPRGRVTAYR
                     ELAERAGIKNGARAAGTALASNPFPLIIPCHRTIRADGFPGEYQGGRAMKKALLEMEG
                     IRFDPSGRAVR"
     gene            complement(1013..2305)
                     /locus_tag="PKK36_10370"
     CDS             complement(1013..2305)
                     /locus_tag="PKK36_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNR94995.1"
                     /translation="MANDKAVLILICITALALGALFATLRSAFTLTVRAGLVRLASRK
                     PSLKDRLSVWGPRWNLLRSALLIFSMLMEVLAIGTAWYLLGGRPGQIITALDFAGLFV
                     ILLLFTFALEVLPLALSESYADRLTLVFLPVAVWLAVALFPLAWPIARLEERIRDRLI
                     RSSDEHDHPTTEDAIRSLVSIPGATRLEEEEREIIRSVFEFGETVTREIMTPRVDVDG
                     VDGQETVHECVQTLKESHYSRFPVFIEDLDDVQGVVHVKDLLRCLSETDGRQLVLKLA
                     KPAPFVPETMPINDLLQLLRSQQAAMAIVVDEYGGMAGLVTIEDIIEELVGDIQDEYD
                     DEEKYIRRLSDGTAIINARVPVCEANEALGIHVPESDEYDSVGGYVFAELGRIPRPGE
                     IVTGPGFRITVQTADARTVQTLHVQKVTAPEGGASAAS"
     gene            complement(2309..2746)
                     /gene="ybeY"
                     /locus_tag="PKK36_10375"
     CDS             complement(2309..2746)
                     /gene="ybeY"
                     /locus_tag="PKK36_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR94996.1"
                     /translation="MNLPRIRKLLIWLMEQAQAVSPAEKWGTVSLTLTDDAGITAANR
                     AYFGKDSPTDVISCAYDPLPGEAGLRAGEVIVNVQRAREEGARRDGMELELALYIAHG
                     CQHLAGRDDAVPAARAAMRRCENAWLRRAQRLGLIGGLTEKDK"
     gene            complement(2676..5051)
                     /locus_tag="PKK36_10380"
     CDS             complement(2676..5051)
                     /locus_tag="PKK36_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNR94997.1"
                     /translation="MAALISKTRSIKSRQRDREERKSARRPWPVACILLVFFLWLATL
                     VTLHVGGTASLTPLAPGQLVTEAVISKVDFKCVDLAETEMSRRRAEDAVLPVFKTGYE
                     KYADAIRTLEKLFRHLGDIRNSGDQYPSRKEKEQAVEKVLDLLGIVLDPAAFLKAVPE
                     GREQEVLGLVSNTLRKAYSGGIVSEADLSSSFKGLVRGSHIQIQQEDGTLRRVSFKGL
                     ASPVSALASAVAAMETALELKPSGAAVWRDLLRPWVVPSLVYQSDTTEQLRQEAADAV
                     PPAETLIRTGTVLVEAGATVDAAVSEKLRAHARRLEELMTPLDRFLRMIADASLLMIA
                     LMIMAGLLQVFKSPLLTTKGHLMLVVILSLLTIVTSKGMLHFSGFEALTSSAVLRYLA
                     PVALTSLLATILLDAGVGMAAGLWTSVAVALLFGRSFSVLMLGLLVTSVSVQTAQNVH
                     RRANIFRVGLWVGLAGSLFALTSAVLMRYPLSLAMMQMAAVFASGMLCAILALMLIPL
                     FEVLFNLTTDIRLLELSDLSNPLLQRLSIEAPGTYHHSMMVANIAHAAAQEIGARALL
                     VRVGAYFHDIGKLTKPEFFVENIQLRENPHDDLAPSMSTLVVTSHVKEGVALARRHKL
                     PQIVIDAIEQHHGTSVVTYFYHRARAQQESEKDGSANAAVKEADFRYTGPRPVSREMG
                     ILALADSVEAASRSMDKPTAARIESLVREIIDRKLLDGQLDNCQLTMADLAAVRKSFI
                     FTLTNMLHGRVPYPQDESRNKQQTAKKPDEPSPNQEAPDLADGTGAGGQPG"
     gene            complement(5051..>5354)
                     /locus_tag="PKK36_10385"
     CDS             complement(5051..>5354)
                     /locus_tag="PKK36_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNR94998.1"
                     /translation="EGQNATCEQMFMFLTRLGFDSKCVVTGDLTQIDLPSGRGSGLME
                     ARLILKNVPEIGFTELGDADVVRHALVQKVIAAYREGRSPENGRPKENRSRPDRSR"
BASE COUNT         1056 a         1668 c         1585 g         1045 t
ORIGIN      
        1 tcctttttca ctgcggaaag ctcgatctct ttccacgata attgacgaag aaccgatttt
       61 ttcgcatttt tgaccgattt tgaccgattt ttattgattt tttgcgttct ttatgcataa
      121 ttgattaata ttcaacatga aactgaaaca aattccccgc acgccttttg gccctgtttc
      181 tttgatctgg ggtcttctga accgtgttcc tctggtttcc cgcgtgattc ttccgcggcc
      241 gggccgaatt aatgaggtaa acattccaga cggctcttgt gcggaaattg atgcggtggc
      301 tgaatccatt tccgccattc tgcaaggcga aaattgcgtc atcccgatcg agcttgccga
      361 tttcagctcc tgttcggatt tccagcagaa aatcctgcgg gcgcaatatc aggttccgcg
      421 cggtcgggtc accgcttatc gggaacttgc cgaaagagcc ggaatcaaaa acggcgcgcg
      481 cgcggccgga acagctcttg cttcaaatcc ctttccgctc atcatcccct gccaccgcac
      541 catccgggcg gacggtttcc ccggcgaata tcagggcgga cgggccatga aaaaagcgct
      601 gctggaaatg gaaggcatcc gcttcgatcc ttccggccgc gcggttcgct gatcaccacc
      661 cgagcaaaga cgcataaggt caatatatcg gtaaccgata tatcggatac cgatatatct
      721 tgcttcaaca tacttgctct gcatttctct ctattcaaaa ttctggcgga cggattcagc
      781 tcaccgcagg atatttcttt catatccgtt tcccgatgcg caccctgacg ggcttgtccc
      841 gccggaacgc atggtcaggc aaacgcggcg ggacccgcag acctttccgc acccgcgaca
      901 caaggtcgcg gggggcggcg ttcccattct ttttccggcc ggcacgaagg ccggccctcc
      961 aaactggaag gctaaaggga cgggctctgt cctgtccttc ggacaatttt gttcaggatg
     1021 ccgccgaagc gccgccttca ggcgcggtga ctttctggac atgcagcgtc tggacggtgc
     1081 gggcatcggc ggtctgaacg gtgatgcgaa acccgggccc cgtcacaatt tcacccggac
     1141 gcggtatgcg gccgagctcc gcaaagacat agccgcccac ggagtcatac tcgtcactct
     1201 ccgggacatg aattccgagg gcctcgtttg cctcgcagac gggaacgcgc gcattgatga
     1261 tggccgtgcc gtcggacagc cggcgaatat atttttcttc gtcgtcatac tcatcctgaa
     1321 tgtcgccgac aagctcttca atgatgtcct cgatggtcac cagtccggcc atgccgccat
     1381 attcatccac gacaatggcc atggcggcct gctgacttcg gagcagctgc aggagatcgt
     1441 tgatgggcat ggtttccggg acgaagggcg cgggcttggc cagtttgagc accagctggc
     1501 ggccgtcggt ttcgctcagg cagcgcagca ggtccttcac atgcacgacg ccctgaacat
     1561 catccagatc ctcaatgaac accgggaagc gggaatagtg cgactctttc agggtttgaa
     1621 cgcattcgtg cacggtttcc tgcccgtcca ctccgtccac atccacgcgc ggcgtcatga
     1681 tttcgcgggt gaccgtttcc ccgaactcaa acacactgcg aataatctcg cgctcctcct
     1741 cctccaggcg ggtggccccg gggatgctca cgagcgagcg gatagcatct tcggtggtgg
     1801 ggtgatcatg ctcgtcggag ctgcggatca ggcggtccct gatgcgttct tcgagacgcg
     1861 caatcggcca tgccagcggg aacagggcca cggccagcca gacggccacc ggcagaaaga
     1921 ccagcgtcag gcggtcagca tagctctcgg acagggccag cggcagcact tccagcgcga
     1981 aggtgaagag caggaggatc acgaagagac cggcaaaatc cagcgcggtg atgatctgcc
     2041 ccgggcggcc cccaagcaga taccaggcgg tgccgatcgc cagcacctcc atcagcatgg
     2101 aaaaaatgag gagcgcggag cggagcaggt tccagcgcgg cccccagacg gacagccgat
     2161 ccttgagcga gggcttgcgt gaagccagcc tcaccagtcc cgcccgcacg gtcagcgtga
     2221 aggccgagcg cagcgtggca aacagggcgc caagcgccag cgccgtgatg cagatcagta
     2281 tcagaaccgc tttgtcattc gccatgggtc atttgtcctt ttccgtcagg ccgccgatca
     2341 aaccaagccg ctgcgcgcgc cgcagccacg cgttttcgca ccggcgcatg gcggcccggg
     2401 ccgccggaac ggcatcgtcg cgccccgcca gatgctgaca tccgtgggca atgtagagcg
     2461 cgagttccag ctccattcca tcgcggcgcg caccctcttc acgcgcgcgc tgcacgttca
     2521 caatcacttc gcccgcgcga aggccggcct cgccgggaag cggatcgtag gcgcagctga
     2581 tcacatcggt cggggaatcc ttcccgaaat acgcccggtt ggcggcggtg atgcccgcgt
     2641 catccgtcag cgtcagcgat accgttcccc acttctcagc cgggctgacc gcctgcgcct
     2701 gttccatcag ccagatcagg agcttcctga ttcggggaag gttcatcggg ctttttcgct
     2761 gtctgttgtt tatttctgct ttcatcctgc ggatacggca cacgcccgtg cagcatgttg
     2821 gtgagcgtga atatgaacga tttgcggacc gccgccagat ccgccatggt cagctggcag
     2881 ttgtccagct gtccatccag aagcttgcgg tcaatgatct cgcgcacgag gctctcgatg
     2941 cgggcggcag tgggcttgtc catggagcgc gacgcggcct cgacggaatc cgccagcgcg
     3001 aggatgccca tttccctgct tacgggccgc ggaccggtgt agcggaaatc cgcctccttc
     3061 acggcggcgt tggcggaccc gtccttttcc gactcctgct gggcacgggc gcggtgatag
     3121 aaataggtca ccaccgaggt gccgtgatgc tgctcaattg catcaatcac aatctggggg
     3181 agcttgtggc gtcttgccag ggccacaccc tccttgacat gcgaggtgac caccagcgtg
     3241 ctcatgctgg gcgcgaggtc gtcatgcgga ttttcgcgca actggatgtt ttccacaaag
     3301 aactccggct tggtgagctt gccgatgtcg tggaaatagg ccccgacgcg aaccagcagc
     3361 gcgcgggcgc cgatttcctg cgccgcggcg tgcgcgatgt tggcgaccat catgctgtgg
     3421 tgataggtgc caggagcctc aatggaaagc cgctgaagga gcggattgct gaggtcggag
     3481 agttccagaa gccggatgtc cgtggtcagg ttgaacagca cctcgaaaag cggaatcagc
     3541 atcagggcca gaatcgcgca cagcatgccg ctggcaaaca cggcggccat ctgcatcatg
     3601 gccagtgaaa gcggatagcg catcagcaca gccgaggtca gcgcgaacag cgaacccgcc
     3661 aggccgaccc agagtcccac gcgaaagata ttggcgcggc gatgcacgtt ctgggcggtc
     3721 tgcacgctca cggaagtgac cagaagaccc agcatcagca cactgaaact gcgcccgaac
     3781 agcagcgcga ccgccacgct cgtccagaga cccgcggcca tgcccacgcc ggcatccagc
     3841 aggatcgtgg ccagaagcga agtgagcgca accggcgcca gataccggag aacggccgat
     3901 gaggtcagcg cctcgaatcc cgaaaagtgc agcatgccct tcgaggtcac aatcgtcagc
     3961 agcgaaagaa taaccaccag catcagatgg cctttggtgg tcagcagcgg tgatttgaag
     4021 acctgcagca ggcccgccat gatcatcagg gcaatcatca gcaggctcgc gtcggcgatc
     4081 attcgcagga accggtcgag cggggtcatc agttcctcca gccggcgcgc atgggcgcgg
     4141 agtttttcgc tcacggcggc atccacggtg gcgccggctt cgaccagcac ggttccggtc
     4201 cggatcagcg tttcggcggg gggaacggca tcggcggctt cctggcggag ctgttccgtt
     4261 gtgtcggact gatagaccag tgacggcacc acccacggtc gaagcagatc ccgccagacc
     4321 gcggcgccag agggcttgag ttccagcgcg gtttccatgg ccgccacggc agaggccagc
     4381 gcggaaaccg gagaagccag gcccttgaac gaaacgcgcc gcagggtgcc gtcttcctgc
     4441 tggatctgaa tatgcgaccc cctcaccagc cccttgaagg agctggaaag gtccgcttcg
     4501 gagacaattc cgccggagta ggcttttcga agcgtgttgc tgaccagacc gagaacttcc
     4561 tgctcgcggc cttcgggaac ggctttcaga aatgcggccg gatccagaac gatgcccagc
     4621 agatccagaa ccttctcaac ggcctgctcc ttctccttgc gcgagggata ctggtcgccg
     4681 gaattcctga tatcgcccag atgccggaaa agtttctcca gcgtccggat cgcatcggca
     4741 tatttctcat agcctgtctt gaagaccggc agaacggcat cctcggcccg gcggcggctc
     4801 atttccgttt ccgcgagatc cacgcacttg aaatccacct tgctgatcac ggcctccgtg
     4861 accagctgac ccggcgccag cggagtcagg cttgcagttc cgccgacatg cagggtcaca
     4921 agcgtggcca gccacagaaa gaagaccagc agaatgcagg ccaccggcca ggggcggcgc
     4981 gccgactttc tttcctcgcg atcgcgctgg cgggatttga tgcttctggt tttggatata
     5041 agcgctgcca ttatctgctc ctgtccggtc tggaacggtt ttctttcggc cgaccgtttt
     5101 ccgggctgcg gccctcgcgg taggcggcaa tcaccttctg caccagcgcg tgccgcacca
     5161 catcggcatc cccaagctcg gtgaatccga tttcgggaac gtttttcagg atgagccggg
     5221 cctccataag gccggacccc cggccggacg gaagatcaat ctgggtgagg tcgcccgtca
     5281 ccacgcattt ggaatcaaat ccgagccggg tcaggaacat gaacatctgc tcgcaggtgg
     5341 cattctgccc ctcg
//
LOCUS       DAOQWR010000155         5330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15731_length_5330_cov_4.360190, whole genome shotgun sequence.
ACCESSION   DAOQWR010000155 DAOQWR010000000
VERSION     DAOQWR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15731_length_5330_cov_4.360190"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKK36_10390"
     CDS             complement(<1..216)
                     /locus_tag="PKK36_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012155223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNR94999.1"
                     /translation="MNAVGVIPARWGSTRLPGKALVNIAGRPLIQWVWERASLAKNLT
                     DLMVATDDRRIAGAVEQFGGRVIMTKQN"
     gene            complement(213..1223)
                     /locus_tag="PKK36_10395"
     CDS             complement(213..1223)
                     /locus_tag="PKK36_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNR95000.1"
                     /translation="MSLSVSRARKLIASFGKQRILVVGDLMLDRFIYGSVSRISPEAP
                     VPVVRVVSESNMPGGASNVAWNIQSLGGRSAVSGFAGDDPAAAELRGVLSKGRVGVDG
                     VLAVPGSCTTVKTRIIAEHQQVCRVDWDAEPDYKNADFDLFAEKLRREIRRSTGVIIE
                     DYGKGVVRQAVVDVVLEAAGKKGVPVGFDPKDNCDLNVHGITVAMPNHREAFMGAGMK
                     ETPVSGSPLKDANLLQAGQRLLKSWSPEFLVITLGSLGMLMMPKKGRTVHIPTRAQEV
                     FDVSGAGDTVVAATVLALAAGATHREAAELANFAAGVVVGKLGTATCSASELLKSVRA
                     GT"
     gene            complement(1204..2004)
                     /locus_tag="PKK36_10400"
     CDS             complement(1204..2004)
                     /locus_tag="PKK36_10400"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNR95001.1"
                     /translation="MGGLVLLWPQGGAAVEFNFPLGEYLVYNIYWGKIPVGEARVRTE
                     WAELDGRRLLSVRFRVVTGKVLSKIYPVNDIHECFIDPQTFLPVRYSKQVNEGRYHAN
                     EVTDYDHEKGQAVMQRAGRDSKKVFEIGPDSRDVISFMYFSRALDFVPGSTSRFEVIS
                     DEKLYELQLDVQKIEDVKIADDSRIRSIRLEPVAKFEGLFVRKGRMWMWISDDDSRLM
                     TKMQAKVPVASVHLLLAEVRSSGENMIIRIPDAKEREEEEAQDVALSQ"
     gene            complement(2139..3281)
                     /locus_tag="PKK36_10405"
     CDS             complement(2139..3281)
                     /locus_tag="PKK36_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNR95002.1"
                     /translation="MKKLILLLLFAISVRGDDSAFALKLYHRLAATEGNVACSPHSIS
                     TALGMAFKGARAETASVMAATLGVPADSAEYDAQMTARIRSLREISSFGGPRMQISNR
                     MWPQEGFYIREVYENALQQVFDADIQTVDFKADAEAARQTINSWIGLATAGRIRELLP
                     AGALNALARLVLTNTIWFKGKWAKAFAETDTREELFHSGSAPVKVPMMKQNGEFLYAD
                     AASFQALEMPYIGERVAMLLLLPRERDGIGDLEQKLDETLLKQCIDNLKKTQVDVSIP
                     RFTAQQNAGLKEVLSEMGMGIAFSARADFSGITGEPELFISDVIHSALVEVNEEGTEA
                     AAATAVVMVKTAARRQPVFRADHPFIYIIRDRQTGAILFMGRLVRP"
     gene            complement(3289..3609)
                     /locus_tag="PKK36_10410"
     CDS             complement(3289..3609)
                     /locus_tag="PKK36_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR95003.1"
                     /translation="MNRRSIFDNITSGLEMEIINTLVHSGPVRIERIVSEGHNSPEDF
                     WYDQDQPEWVILIQGSATLEVEGRTEPVELRPGDYVNIPARCRHRVARTDESQHTIWL
                     AVYY"
     gene            3705..4448
                     /locus_tag="PKK36_10415"
     CDS             3705..4448
                     /locus_tag="PKK36_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95004.1"
                     /translation="MVAKKQIAVLTAVYIGLIGLFFLYWGIHRISPEFHMNYMQGEDR
                     AAEWLTFIGFAGAAAMSWATLRFRAVMGRKTIAFFALLGLFMFVCAGEEISWGQRIFG
                     FGTPEAMVEVNEQNEFNLHNLSFEHLHPKDIVSWFMKIYGIILPLVFLRRYIRGENEI
                     FKFIPAPVCTPAFVIPELINVFEDQLAAFVARYFGPFTLPPVCPSVSVLIKRQCEELI
                     EMYWGLAIFMALLMIWAAWKQSVRPASAQ"
     gene            complement(4426..4944)
                     /gene="ruvC"
                     /locus_tag="PKK36_10420"
     CDS             complement(4426..4944)
                     /gene="ruvC"
                     /locus_tag="PKK36_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR95005.1"
                     /translation="MPAPAGVRIMGVDTSLRSTGIGVVEARGNTFRIIEFGTIKNPQS
                     RIHSESLAFLHQSVMEWIGRCSPDAVAIEGSFYFRNAATAMTLGQARGVVMAAAAQKH
                     LAVYEYAPRRVKQALTGSGTASKEQIIRMIVSLLGIKGTDPTDDEADALALAVCHLQA
                     RSSFGVIAPKPV"
     gene            complement(4944..>5330)
                     /locus_tag="PKK36_10425"
     CDS             complement(4944..>5330)
                     /locus_tag="PKK36_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR95006.1"
                     /translation="NLSATGSVSRLFERRGQIIVSGDAATEDQLMELALDAGADDMTN
                     EGDHFEILTSPASFMNVADALNKAGIAMASSELTMLPLNWTPVSDKGQAASLLKFVEA
                     LDDLDDVQNVWHNADISDEMMTEIEG"
BASE COUNT         1069 a         1625 c         1487 g         1149 t
ORIGIN      
        1 gttctgcttt gtcatgatca cgcgcccgcc gaactgttca accgcgccag caatgcgcct
       61 gtcatcggtt gcaaccatca aatcggtgag attctttgcg aggcttgcgc gctcccagac
      121 ccactgaatg agcggccggc ccgcaatgtt caccagcgcc ttgcccggaa gccgggtgga
      181 gccccagcgt gcgggtatta cgccgacggc attcacgtgc ccgccctcac gctcttcagt
      241 agttctgacg ccgagcacgt ggcggtgccg agcttgccca ctacaacacc cgcggcaaaa
      301 ttggccagtt cggcggcttc gcgatgagtg gcgcctgccg ccagcgccag cactgtggcg
      361 gcaaccacag tatcgcctgc gccgctcacg tcgaacactt cctgtgcgcg cgtgggaata
      421 tgaacggttc tgcccttttt cggcatcatc agcattccga gactccccag cgtgatcaca
      481 agaaattccg gcgaccagga cttgagcagg cgctggccgg cctgcagcag gttggcgtcc
      541 ttcagcggac ttccggatac gggggtttcc ttcatgcccg cgcccataaa cgcctcgcgg
      601 tgattcggca tggccacggt gatgccgtgg acattcaggt cgcagttgtc ttttggatca
      661 aaaccgacgg gaacgccctt tttccccgcc gcctccagca ccacatcaac cacggcctgc
      721 cggaccacac ctttgccata gtcctcgata atgacgcccg tggagcgacg gatttctctg
      781 cggagctttt ccgcaaacaa gtcgaaatca gcatttttgt agtcgggttc agcgtcccag
      841 tccacgcggc acacctgctg atgttcggca atgatgcggg ttttgaccgt tgtacagctg
      901 ccgggcacgg cgagcacgcc gtccactcca acgcggcctt tcgaaagcac ccctcgcagt
      961 tccgcggccg ccgggtcgtc gcctgcaaac ccgctcacgg cgctgcggcc ccccagcgac
     1021 tgaatattcc aggccacgtt gctggcgccg cccggcatat tgctttcgct cacgacgcgc
     1081 accacgggaa ccggggcttc cggggagatt cgactcaccg atccataaat gaagcggtcg
     1141 agcatcaaat ccccgaccac aagaatccgc tgttttccaa atgaagcaat cagcttcctg
     1201 gccctactga ctgagagcga catcctgcgc ctcctcttcc tcacgttctt tcgcatccgg
     1261 gattcgtata atcatgttct cgcccgagct tcgcacctcg gccagcagca gatgaacgct
     1321 ggccacgggc acttttgcct gcatcttcgt catcaggcgg ctgtcatcat ccgatatcca
     1381 catccacatc cggccttttc ggacaaacag cccctcgaac ttggcgaccg gctcaagccg
     1441 gatgctgcgg atgcggctgt catcggcgat tttcacgtct tcaatcttct ggacatccag
     1501 ctgcagttcg taaagctttt cgtccgaaat cacctcaaac cggcttgtgc ttcccgggac
     1561 gaaatccagc gcgcgggaaa aatacatgaa tgaaatcaca tcgcggctgt ccgggccgat
     1621 ctcaaaaacc tttttactgt cgcggcccgc ccgctgcatg acggcctgcc ctttttcgtg
     1681 atcgtagtcg gtgacctcat tggcgtggta ccggccttcg ttgacctgct tggagtaccg
     1741 caccgggaga aaggtttgcg gatcaatgaa gcactcatgg atgtcattca ccgggtaaat
     1801 tttgctgagc acttttccgg ttaccacgcg gaaacgaacg ctgagaagcc ggcggccgtc
     1861 caattcggcc cattccgtgc gcacccgggc ctcgccgacg gggatctttc cccagtaaat
     1921 gttgtagacc aggtactctc ccagcgggaa attgaattcg acggccgctc ccccctgcgg
     1981 ccacagcaga accagaccgc ccataagcag gaaccacctt ttccaaaaag aagtcatgcg
     2041 gcagaatcta gcgttttttc caatgattgg aagttttttc gggaaaagtt ccaatgattg
     2101 gaagtttttc aagcgaaagt tccaatggtt ggaacttttt acggccggac cagccggccc
     2161 atgaagagaa tcgcgccggt ctgccggtca cgaatgatgt aaatgaacgg atggtcggcg
     2221 cgaaagaccg gctggcgtct tgctgcggtt ttgaccatga caaccgccgt ggccgcggcc
     2281 gcttcggtgc cctcctcatt cacctcgacc agcgcggaat ggatcacatc cgagatgaag
     2341 agttccggct cgccggtgat gccgctgaaa tcggcccggg ctgagaacgc gatacccatg
     2401 cccatttcag agaggacttc cttcaggccg gcgttctgct gcgcggtgaa gcgagggatg
     2461 ctgacgtcca cttgtgtttt tttcaggttg tcaatgcact gcttcagcaa agtctcgtcc
     2521 agcttctgtt ccagatcgcc gatcccgtcc cgttcgcgcg ggagcagaag cagcatggca
     2581 acgcgctcgc cgatgtacgg catttccagc gcctggaaac tggcggcatc ggcataaaga
     2641 aactcgccgt tctgcttcat catcgggact ttcaccggcg ctgatcctga atgaaaaagt
     2701 tcttcgcgcg tgtcagtctc agcaaaagcc ttcgcccatt tccccttgaa ccagattgtg
     2761 ttggtgagaa ccagccgcgc aagagcattg agcgccccgg ccgggagaag ctccctgatc
     2821 cggccggccg tggccaggcc gatccagctg ttgattgtct gccgggccgc ttcggcatcc
     2881 gccttgaagt ccacggtctg gatgtcggca tcgaacacct gctgcagcgc attttcatag
     2941 acctcccgaa tatagaaacc ctcctgcggc cacatgcggt tggatatctg catccgcggg
     3001 ccgccaaacg atgatatttc gcgcagcgac ctgatccggg ctgtcatctg cgcatcgtac
     3061 tccgcggaat cggccggaac gccgagggtg gcggccatga ccgaggcggt ttccgcgcgc
     3121 gcccccttga aggccatgcc cagggcggtg ctaatactgt ggggcgagca cgccacattt
     3181 ccctccgttg cggcgagccg gtgatagagc ttgagcgcga aagcgctatc gtcccctcgg
     3241 acagagatgg caaacaaaag aagtagaatc agttttttca taatcacctc aataataaac
     3301 ggccagccag atggtatgct gagactcatc ggtgcgcgcc acacggtgcc ggcagcgggc
     3361 aggaatgtta acatagtccc ccggccggag ttcgaccggt tcagtccgtc cttctacttc
     3421 cagcgtggcg gagccctgaa tcaggatcac ccattcaggc tggtcctggt cgtaccagaa
     3481 atcctccggg gagttgtgtc cctcggaaac aatgcgctcg atccgcacag gtccggaatg
     3541 cacaagcgtg ttgatgatct ccatttccag gccggaagtg atgttgtcaa agatgctgcg
     3601 ccggttcatt ccgcaataat aacccgggcc cggcccagta caactcgaaa acttccggat
     3661 actggggata gacagaatca gcgaaggaag gtagtttatg ccgcatggtt gcaaagaaac
     3721 agattgctgt gctcacggcc gtgtatatcg ggctgatcgg actctttttc ctgtactggg
     3781 ggattcacag aatctcgccg gagtttcaca tgaattacat gcagggagaa gaccgtgccg
     3841 cggaatggct gacattcatc ggttttgccg gggccgcggc gatgagctgg gcgaccctcc
     3901 gtttccgggc ggtgatgggg agaaagacca ttgcgttttt tgcgctgctg gggctcttca
     3961 tgtttgtttg cgccggagag gaaatcagct ggggtcagcg gatattcggg ttcggcacgc
     4021 cggaggccat ggtggaggtc aacgagcaga acgaattcaa cctgcataat ctttcctttg
     4081 aacaccttca cccgaaggat attgtctcgt ggttcatgaa gatatacggg atcattctcc
     4141 cgctggtgtt tctccggcgc tatattcgcg gggaaaatga gatattcaag ttcatcccgg
     4201 cgcctgtctg caccccggcc tttgtgattc ctgaactgat taacgtgttt gaggatcagc
     4261 ttgcggcttt cgttgcgcgg tatttcggcc cgttcacgct gccgccggtc tgtccgtctg
     4321 tttccgtcct gatcaagcgc cagtgcgaag aactgatcga gatgtactgg ggtctcgcca
     4381 tattcatggc cctgctgatg atctgggccg catggaaaca aagcgtcaga ccggcttcgg
     4441 cgcaataacc ccaaaagacg aacgggcctg aagatggcag accgccagcg ccagcgcatc
     4501 ggcctcgtca tctgtcggat cggtcccttt gattcccagc aaactgacaa tcatgcggat
     4561 gatctgctcc ttggaggctg ttccggaacc ggtcagggcc tgcttgaccc ggcgcggcgc
     4621 atattcgtag accgcaagat gcttctgcgc ggcggccgcc atcacaaccc cgcgggcctg
     4681 acccagcgtc atggctgtgg ccgcattgcg gaagtagaat gagccctcaa tggccacggc
     4741 atccggagag cagcggccaa tccattccat tacggactga tgcaaaaaag cgagagattc
     4801 ggagtgaatc cggctctggg gattcttaat ggtgccgaat tcaatgatcc tgaaggtgtt
     4861 tccccgggcc tcgaccactc caatgccggt ggaacgcagg gaggtgtcca cccccataat
     4921 gcgcactccg gcaggggccg gcatcagccc tcaatctccg tcatcatttc gtcactgatg
     4981 tcggcattgt gccatacatt ctgcacgtca tccaggtcat cgagcgcctc gacaaacttg
     5041 agaagcgagg ccgcctgccc tttgtcggac acgggcgtcc agttcagcgg gagcatcgtc
     5101 agttcggaac tggccatcgc gatcccggcc ttgttcaagg catcggccac attcatgaac
     5161 gaagccggcg aggtcaggat ttcaaaatga tcgccctcgt tggtcatgtc gtcggcgccg
     5221 gcatccaggg ccagttccat gagctggtct tcggtcgccg catccccgct cacaatgatt
     5281 tgcccgcggc gctcaaacag ccggctgacg cttcccgtcg ccgagagatt
//
LOCUS       DAOQWR010000156         5307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15821_length_5307_cov_5.041127, whole genome shotgun sequence.
ACCESSION   DAOQWR010000156 DAOQWR010000000
VERSION     DAOQWR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_5307_cov_5.041127"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKK36_10430"
     CDS             <1..276
                     /locus_tag="PKK36_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95007.1"
                     /translation="LVVDGDLFIFSDEIKVEMKKASEGALKGILGYTEDDVVSSDFIG
                     EECTSVFDAKAGIALNDRFVKLVAWYDNEWGYSCKCLDLMVHMARKA"
     gene            345..1601
                     /locus_tag="PKK36_10435"
     CDS             345..1601
                     /locus_tag="PKK36_10435"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNR95008.1"
                     /translation="MNKLTVRDVDCKGRRVLMRVDFNVPVKDGVVQDDTRITAALPTI
                     RYVLDQGASVVLMSHLGRPKAKGYEAAFSLKPVAARLQELLSRPVQFGPDSIGAETLA
                     MAQAMKPGDVMLVENTRFYADEGKAKLPETATEEEKKAAKAEMKKKQAELAAKLAPLG
                     DGEIFVNDAFGSAHRAHASTALVCKYYKRNVAGFLMEKEIEYIGKALASPERPFVAIL
                     GGAKVSDKVNVINNLLTKVDALLIGGAMAYTFYRAQGIPTGKSLVEEDKIDLAKNLLA
                     KAEELKVKMLLPVDHVIADAFDANAKTRTVGLKGIEDGWMALDIGPETAKVYAAEVKK
                     AKTVVWNGPMGCFEMKPFAEGTLEVAKAIAATDCLSIIGGGDSVSAVNKSGLADKMSH
                     ISTGGGASLEFLEGKTLPGVAALNDK"
     gene            1618..2382
                     /gene="tpiA"
                     /locus_tag="PKK36_10440"
     CDS             1618..2382
                     /gene="tpiA"
                     /locus_tag="PKK36_10440"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNR95009.1"
                     /translation="MRKPIVAGNWKMNKTVAEAIDLAESIKRDLAACQKVDVVLCPPF
                     TALSAVGNVISGTQIDLGAQNMHWEKSGAYTGEISPEMLRDLFCHYVILGHSERRQYF
                     GETDEIVNRKAKTALALNLHPIVCVGETLEERKAGRTNEVVEKQVRGSLADFGARGWL
                     ESIIAYEPVWAIGTGLTASPAQAQEVHALIRSVLQDISDEHVAKSVRIQYGGSVKPGN
                     AAELFGQPDIDGGLIGGAALDASSFIDIVLAAQETA"
     gene            2403..2801
                     /gene="secG"
                     /locus_tag="PKK36_10445"
     CDS             2403..2801
                     /gene="secG"
                     /locus_tag="PKK36_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNR95010.1"
                     /translation="MQEIIRAVLLVVEGLSCLLLIGLILLQKSQGGGLGGAFGGGMGE
                     TLFGSRAGNVLTKGTIILSIIFMANTVVLAIMFTGGREASLMQTMPAAPVQTMPAASP
                     AAEAAPAPAAAPVMVEDFAVPAEESAAPAE"
     gene            2810..4504
                     /locus_tag="PKK36_10450"
     CDS             2810..4504
                     /locus_tag="PKK36_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR95011.1"
                     /translation="MTALKPHRLLAYGVLSLALCGCGESSFQNSPGAFRSSTTRIRGF
                     DPVRAGDVASQLAMGKVYEGLLQYSYLDRPYRLEPLLAEEMPDVSPDGLVFTFRLKRG
                     VHFHDDPCFPGGKGRELTAEDFIYSIKRAADKKNLSTGWWAFNGRIQGLNEFREAAAG
                     ESPTDYSVPVAGLTAPDPHTLQITLTRPFPQLLWILAMPYAYAVPREAVEYYGENFVS
                     HPVGTGPFILSEWRRNYRLEYRRNPAWHGGAPGLERIVEYMIGDSSTRWLAFLTGQLD
                     FYKDISHDNWDVIFRDGGRLAPELAARGIRASGIAGLDTYYIGFNMDDPVVGHNKKLR
                     KALTCAFESEAWIKYYNHRVTRARGPIPPGVEGYSDEPALHPYDLEKARKLLAEAGWP
                     DGRDPQTGKRLELTLELGQTDTEIRESTELFIGFMNKLGVVINPSYNNKPAFFKKIEQ
                     REAQMFRLSWFADYPDAENFLQLFYGPNASPGPNRVNYRRPEYDRLYEEIRVMQDGPE
                     RTELYRRMAAMVEEDCPWIFMHHPMAYVLHHDRLRNYAPHDFPYGMEKHFRLEERP"
     gene            4501..>5307
                     /locus_tag="PKK36_10455"
     CDS             4501..>5307
                     /locus_tag="PKK36_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95012.1"
                     /translation="MSRYILRRLLHMIPALAGVILLTFVLFNVVGGSPATMVLGMHAT
                     AQSLEDYETARGCSLPLFFGRETTTRAFEDMDAARASAAWKLKDAVLSKGTYGLPLAL
                     DIEGPVRWIFRGRVTGAASLVCERLSGEKIAVPLRRQAEFTALPGDRYSLSVEQGTVS
                     IKNLRLRRLQDNPFHSQLVLYLRQLARLDFGISLEANQKVSALLKSGILPSLCLTAPI
                     LFGGTAVAIFMALLCAYFRDRFFDRLLVVLATVLMSVNYIIWVIAGQYFFA"
BASE COUNT         1103 a         1519 c         1643 g         1042 t
ORIGIN      
        1 ctggtggttg atggcgacct atttatcttc tcagatgaaa tcaaggtcga aatgaagaag
       61 gcctcggaag gcgcgctgaa gggcattctc ggctatacag aagacgatgt ggtctcctcc
      121 gatttcatcg gcgaagagtg cacctcggtg ttcgacgcca aggccggcat cgcgctgaac
      181 gaccgctttg tgaagctggt ggcctggtat gacaatgagt ggggctactc ctgcaaatgc
      241 ctggacctga tggtccacat ggccaggaag gcctgaacaa catattgaac ctgcgcgggg
      301 cggactccgg tccgccccgc ttccaaacag gaatacggac gaatatgaac aaactgactg
      361 ttcgcgatgt ggactgcaag ggcaggcgtg tgctgatgcg cgtggatttc aatgtgccgg
      421 tgaaagatgg cgtggtgcag gatgacaccc ggatcaccgc cgcgcttccg accatccggt
      481 acgtgctgga tcagggcgcg tccgtggttc tgatgagcca tctggggcga ccgaaggcaa
      541 agggctacga ggccgccttc agtctcaaac cggtggccgc ccgcctgcag gaactgctca
      601 gccgcccggt ccagttcggg ccggacagca tcggcgccga gactctggcc atggcgcagg
      661 ccatgaaacc cggcgatgtt atgctggtcg agaacacacg tttttatgcc gacgaaggca
      721 aagcgaagct gcccgaaacc gcgacggaag aagagaaaaa ggctgccaag gccgaaatga
      781 agaagaagca ggctgaactg gccgcaaagc tggcgccgct gggcgacggc gaaatctttg
      841 tcaacgacgc cttcggctcg gcgcaccggg cccatgcgtc caccgcgctc gtgtgcaagt
      901 attacaagcg gaatgtcgcc ggatttctga tggagaagga aatcgagtat attggcaagg
      961 cgctggcaag tccggagcgg cccttcgtgg cgatcctcgg cggcgccaag gtgagcgaca
     1021 aggtgaacgt catcaacaac ctgctgacca aggtggatgc gctgctgatc ggcggagcca
     1081 tggcctacac gttctatcgg gcgcagggca tccccaccgg caagtctctg gtggaggagg
     1141 acaagattga ccttgcaaag aacctgctgg caaaggcgga ggagctcaag gtaaagatgc
     1201 tgctcccggt ggaccacgtc atcgcagatg ccttcgatgc aaacgccaaa accagaacgg
     1261 tcggcctcaa gggcattgaa gacggctgga tggcgctgga catcggaccg gaaacagcga
     1321 aagtttatgc cgctgaggtg aaaaaagcaa agaccgtggt gtggaacggg ccgatgggat
     1381 gcttcgagat gaagcctttt gcagagggaa ctcttgaggt tgcaaaggcg atcgcggcga
     1441 cggactgtct gagcatcatc ggtggcggcg acagtgtcag cgccgtgaat aaaagcggac
     1501 tggccgacaa aatgagccat atcagcaccg gcggcggcgc cagcctcgag ttccttgagg
     1561 gcaagactct gccgggcgtg gccgcgctga acgataagta gatcaggaga gaagaagatg
     1621 agaaagccga ttgttgccgg aaactggaaa atgaacaaaa ctgtggctga ggccattgac
     1681 ctcgccgaga gcatcaagcg cgacctcgcc gcctgccaga aagtggatgt cgtgctctgc
     1741 cccccgttca ccgcgctcag cgccgtcggc aacgtcatca gcggcactca gattgatctg
     1801 ggcgcgcaga acatgcactg ggagaagtcc ggcgcctaca ccggcgaaat atcgcccgag
     1861 atgctgcgcg accttttctg tcactatgtc attctcggcc acagcgagcg ccggcagtat
     1921 ttcggcgaga ctgacgaaat cgtcaaccgc aaagccaaaa ccgctctcgc cctgaatctg
     1981 catccgattg tctgtgtcgg tgaaacactt gaggagcgca aggccggccg gacaaacgag
     2041 gtggtggaga agcaggtgcg cggaagtctt gcggacttcg gcgcgcgcgg ctggcttgag
     2101 agcatcatcg cctatgaacc ggtctgggcc attggcacgg gactgacggc ttcgcccgcc
     2161 caggcgcagg aagtgcatgc gctgatccgc agcgtcctgc aggatatctc cgacgagcat
     2221 gttgcaaaat ccgtgcggat tcagtatgga ggtagtgtga agcccggaaa cgcggccgag
     2281 ctttttggac agcccgacat tgacggagga ctgattggcg gagcggcgct ggatgcgtcc
     2341 tcctttattg atattgtcct ggcggcccag gaaaccgcgt aaattcagat tggaaataga
     2401 aaatgcaaga gattatccgt gcggtactgc tggtggttga aggtttgagc tgtctgcttc
     2461 tgatcggtct gatcctgctg cagaagtcac agggcggcgg ccttggcggc gccttcggcg
     2521 gcggcatggg cgaaaccctg ttcggttcgc gcgcaggaaa tgtgctgacc aagggaacca
     2581 tcatcctgag tatcattttc atggccaaca cggtggtgct ggccatcatg ttcacgggcg
     2641 gccgcgaagc ctccctgatg cagaccatgc ccgccgcgcc tgtgcagacg atgcctgcgg
     2701 cgtctcccgc ggcggaagct gcgccggccc cggccgcggc gccggtgatg gtggaggatt
     2761 ttgcggttcc cgcggaagaa agcgctgcgc cggcagaata agcggcatca tgaccgcctt
     2821 gaaaccccat aggctccttg cgtatggggt tttgtctttg gccctttgcg gatgcgggga
     2881 atcttccttc cagaacagtc ccggcgcttt ccgcagttca accactcgaa tccgcggatt
     2941 cgatcctgtc cgggccggcg atgtggcttc gcagcttgcg atggggaagg tctacgaggg
     3001 gttgctccag tattcctatc tggaccgccc ttatcgtctg gaacccctgc tggccgagga
     3061 aatgcccgat gtttcgcccg atggactggt tttcaccttc cgtttgaagc ggggagtgca
     3121 tttccacgac gacccctgct tccccggagg gaagggccgc gagctgacgg cggaagattt
     3181 catctattcg atcaagcggg ccgccgacaa aaagaacctt tccaccggct ggtgggcgtt
     3241 caacggccgc attcagggtc tcaatgagtt tcgcgaggcc gccgccggcg aaagcccgac
     3301 tgactattcc gtgccggttg cgggattgac ggctccggat ccgcacacgc ttcaaatcac
     3361 gctgacccgt ccatttcctc agctgctgtg gatactggcg atgccatacg cctatgcggt
     3421 cccgcgcgaa gcggtggaat actacgggga gaattttgtc agccatcccg tcggcaccgg
     3481 gcccttcatc ctcagcgagt ggcgacgcaa ctaccggctt gaataccggc gcaaccccgc
     3541 gtggcacggc ggcgcgcccg ggctggagcg gattgtggaa tacatgatcg gagattcctc
     3601 cacgcgctgg ctggccttcc tgaccgggca gctggatttc tacaaggaca tttcccatga
     3661 caactgggat gtcatcttca gggacggagg tcgcctcgcg cccgagctgg ccgcgcgggg
     3721 catccgcgca tccggcatcg ccggcctgga cacatattac atcggcttca acatggatga
     3781 tccggtcgtg ggccataaca aaaaactgcg gaaggctctg acctgcgcgt ttgaatccga
     3841 agcatggatc aaatattata atcaccgggt tacgcgcgcc cgcggaccga ttccgccggg
     3901 tgtggaagga tattctgacg aacccgccct ccatccttat gatctggaga aggcccggaa
     3961 gcttctggcc gaggccggct ggccggacgg acgcgatccg cagaccggaa aacgcctgga
     4021 actcacactc gaactggggc agaccgacac agagatccgc gaatccacgg aactcttcat
     4081 tggattcatg aacaaactgg gcgtggtgat caacccgtcc tacaacaaca agccggcttt
     4141 cttcaaaaaa atcgaacagc gcgaggcgca gatgttccgg ctgagctggt ttgccgatta
     4201 tcctgacgca gaaaacttcc tgcagctttt ttacggaccg aacgcatcgc ccgggcccaa
     4261 tcgcgtcaac taccgccgtc cggaatacga caggctttac gaagagattc gcgtaatgca
     4321 ggacgggccg gagagaacgg aactctaccg gcgcatggcg gccatggtcg aggaggactg
     4381 tccctggata ttcatgcatc atccgatggc ctatgtgctg catcacgacc gcctgcggaa
     4441 ttacgcgccg catgatttcc cgtacggcat ggaaaagcat ttccggctgg aggagcggcc
     4501 atgagccggt acatccttcg ccgcctgctg cacatgatcc cggctcttgc cggcgtgatc
     4561 cttctcacct ttgtgctctt taacgtcgtt ggcggcagtc ccgccactat ggttcttggc
     4621 atgcatgcca cagcgcagtc ccttgaggat tatgaaacag cccgcggatg cagtctcccg
     4681 ctgttcttcg gccgtgaaac aacaacccgc gcatttgagg atatggatgc cgcgcgcgct
     4741 tcggcagcat ggaaactcaa ggatgcggtt ctgtcgaagg gaacgtacgg gcttccgctg
     4801 gcgcttgata tcgaaggccc ggtccgctgg atattccgcg gccgtgtcac cggcgcggca
     4861 tcccttgtct gcgaacgcct gtccggagaa aagattgcgg ttccgcttcg caggcaggca
     4921 gaattcacgg ctcttcccgg cgaccgctac agtctttccg tggagcaggg gacggtcagc
     4981 ataaaaaacc tgcgtctgcg gaggctgcag gacaatccgt tccattcgca gcttgtgctt
     5041 tatctgcgcc agcttgcccg tctggatttc ggaatctcgc tggaggccaa ccagaaggtc
     5101 tccgcgctgc tgaaaagcgg catcctcccg tcgctgtgcc tcacggcgcc cattctgttc
     5161 ggcggcaccg cggttgcaat cttcatggcg ctgctttgcg cgtatttccg tgaccggttc
     5221 tttgaccgcc tgctggtggt gctggccacg gtgctgatga gcgtgaatta tatcatctgg
     5281 gtcattgccg gacagtattt tttcgcg
//
LOCUS       DAOQWR010000157         5292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15876_length_5292_cov_4.619439, whole genome shotgun sequence.
ACCESSION   DAOQWR010000157 DAOQWR010000000
VERSION     DAOQWR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15876_length_5292_cov_4.619439"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKK36_10460"
     CDS             complement(<1..442)
                     /locus_tag="PKK36_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95013.1"
                     /translation="MAGILFFAPALCARAGMDLAAALYEEEDWSACRTESLRVLQQEP
                     ENGSARLFAALAALRLGPRNGEEPGITPKKVSRSSTLQSGKCTSSKGVQMEGGAPRRR
                     CLSARLELEALASASNDREVMAAASYECGRLALAVLRQLQRTNGR"
     gene            complement(497..787)
                     /locus_tag="PKK36_10465"
     CDS             complement(497..787)
                     /locus_tag="PKK36_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95014.1"
                     /translation="MKKVMIAMLIAVVLLGSIPVQAASGSGRGGFMGFIAGCCFGIRA
                     GADYNSGKEIHWREWCMLIPYVNIVFAIWNGIDGANGVTRADYASQYGGMFY"
     gene            1006..2079
                     /locus_tag="PKK36_10470"
     CDS             1006..2079
                     /locus_tag="PKK36_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95015.1"
                     /translation="MARKASAPDFRVLMLFNIDPEWETADIKATVASSRELADGLSQA
                     GFKTVMVPVDKPDLEKVLRPYSPEACIVFNWCESLPGIEHSEGAVVRELERLGFIYTG
                     STAQALDLSINKPLVKQCLGEWDVPTPRWRVFPNLRNAHQWNLFPAIVKPAHEHCSLG
                     LSPQSVVTTNEELKQQISKLITEMKQPALVEDFVDGREFHVALWGNDELEVLPAAEMD
                     FAGISDMHQRLCDYDAKFTPDSELYNKIGTLLPAPLTAEEKENLESTCRAAYRATGCR
                     DYARIDLRLRDGIFNVLDVNPNPDISSDASMACAADTLGISYGELGRRIVEMALERHP
                     VLPARKPRPANAPVSADAVASPG"
     gene            2279..2518
                     /locus_tag="PKK36_10475"
     CDS             2279..2518
                     /locus_tag="PKK36_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HNR95016.1"
                     /translation="MNTKLTLRMDESLVETAKEEAASRGKSVSQMVGDFFGSLKSTAH
                     SKRNRPPITASLLGVLRNRKLSEADYKKHLREKYR"
     gene            2515..2928
                     /locus_tag="PKK36_10480"
     CDS             2515..2928
                     /locus_tag="PKK36_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNR95017.1"
                     /translation="MRIIVDTNVVLDVLLDRKPFANAAAGVFSLIEESKFEGMLCATT
                     VTTIDYLLSQAFSRPKSLETLRRLLELFEIAPVNRPVIEEAFRSKITDFEDAVLEQAG
                     RLAGADAIVTRNTRDFRNSAIKAIGPDELLSAFIE"
     gene            3012..>3624
                     /locus_tag="PKK36_10485"
     CDS             3012..>3624
                     /locus_tag="PKK36_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR95018.1"
                     /translation="MPHQSETKNIAVFCDFENVALGVADAKYAKFDIMKVLERLLLKG
                     NIVVKKAYCDWGRYRDFRPAMHEASFELIEIPHVRMSGKNSADIRMVVDALDLCYTKE
                     HVDTFVVISGDSDFSPLVSKLRENNKTVVGVGVKNSTSDLLIANCDEFIFYDDLVRAQ
                     PKPARPRAAASKSAKTPAKAAASTDDKKLEAFNLIVATYEALVE"
     assembly_gap    3625..3724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3725..3979
                     /locus_tag="PKK36_10490"
     CDS             <3725..3979
                     /locus_tag="PKK36_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HNR95019.1"
                     /translation="KKLEAFNLIVATYEALVEERGEEENIWGSMIKQTLKRRNPGFSE
                     SFYGFRSFGQLLEEAGQQGFLELERDEKSGGYIIKGCRQA"
     gene            4274..4483
                     /locus_tag="PKK36_10495"
     CDS             4274..4483
                     /locus_tag="PKK36_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNR95020.1"
                     /translation="MSEIVFEVTQEADGGFVAECLNESIFTQADTWDELRRNVQDAIS
                     AFYFDRPHPSVVRLHLVRDEILAAV"
     gene            4480..4719
                     /locus_tag="PKK36_10500"
     CDS             4480..4719
                     /locus_tag="PKK36_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR95021.1"
                     /translation="MKLPRDLSGTDLVQALSRSWAYQKVHQSGSHIILQTETPFPHRI
                     AVPAHAVLRLGTLNAILRAVAAHKGVGREDILKSL"
     gene            complement(4901..>5292)
                     /locus_tag="PKK36_10505"
     CDS             complement(4901..>5292)
                     /locus_tag="PKK36_10505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR95022.1"
                     /translation="NELNKQRNFQEAWYQYQAVLQYSPDEFWALYQMVILAMQSNQPD
                     LAGQFLEQGMAAYPDAPMFRALRGKYRGTLGDPAAACADYEYAVGLLPDHPMLWREYA
                     FFLRQAGRAAEADEAEAKAEKLQGAKL"
BASE COUNT         1143 a         1582 c         1394 g         1073 t
ORIGIN      
        1 accggccgtt cgtcctttga agctggcgga gaaccgccag ggccaggcgg ccgcattcat
       61 aggaagccgc ggccatcacc tcgcggtcgt tcgacgccga tgcaagcgcc tcaagttcca
      121 atcttgccga cagacaacgg cgtcgcggag ctccgccctc catttgaaca cccttcgatg
      181 atgtgcattt ccccgactgg agggtcgagc tccgcgagac cttctttggg gttatgccgg
      241 gttcttcacc gttccggggg cccagccgca gggccgccag cgccgcaaac agccgcgcgc
      301 tcccgttctc cggttcctgc tgaagaaccc gcaggctttc cgtgcggcac gcggaccagt
      361 cctcttcctc atacagggcg gccgccagat ccatgcccgc gcgcgcgcaa agagccgggg
      421 caaaaaaaag gattccggcc attaagaccg gaatcccctt cactctgttt ctaaaaaacc
      481 gggccacgcc ttcggtttag tagaacattc cgccgtactg cgatgcgtag tcagcgcggg
      541 tcacgccatt ggcgccgtca atgccgttcc agatcgcgaa cacgatgttc acatagggaa
      601 tcagcatgca ccattcacgc cagtggattt ccttgccgga gttgtagtca gcgccggcgc
      661 ggataccaaa gcagcatccg gcaataaagc ccatgaagcc gccgcgtccg ctgccggagg
      721 ccgcctgcac cggaatggag cccagcagaa ccaccgcaat cagcattgca atcattacct
      781 tcttcattca cgcctcctta tcgttaagcg tatgagcaag gctagcgatc acgccatgcc
      841 tttacaagca ctttttgccg gatttatgaa tccggcattt tctcaaaaaa accgcttgca
      901 caatgttcat tttgcgggta agtgagagat caggaatcaa caggtggtgg ttgaagttat
      961 tgacaagtca atatattgta ggcttccgag tggaaggaga cgctgatggc gcgtaaggct
     1021 agtgccccgg atttccgggt tttaatgctg ttcaacatag atccggaatg ggaaaccgcc
     1081 gatatcaagg cgacggttgc ctccagccgt gaactcgccg acggtctctc acaggccggg
     1141 ttcaaaaccg tcatggtccc cgtggacaaa cccgatcttg aaaaggtcct ccgcccctat
     1201 tctcccgaag cctgcattgt tttcaactgg tgcgaaagcc tgcccggcat cgaacacagc
     1261 gaaggcgccg tcgtccgcga gctggaacgg ctcgggttca tctacaccgg ctccacggcg
     1321 caggcgctgg atctttccat caacaagccg ctggtcaagc agtgcctcgg cgagtgggat
     1381 gttccgaccc cccgctggcg cgtcttcccc aacctccgca acgcccatca gtggaacctg
     1441 tttcccgcca tcgtgaaacc ggcccacgag cactgcagcc tcggcctttc gccgcagtcc
     1501 gttgtgacca ccaacgaaga actcaagcag cagatcagca agctcatcac agaaatgaaa
     1561 cagcccgcgc tggtcgagga tttcgtggac ggccgcgagt ttcacgtcgc cctctggggc
     1621 aacgatgaac tcgaagtcct gcccgccgcc gaaatggact ttgccggaat cagcgacatg
     1681 catcagcgcc tgtgcgacta cgacgccaag ttcacgccgg attccgaact ctacaacaaa
     1741 atcggcaccc tgctccccgc cccgctgacg gcggaggaaa aggaaaacct ggaaagcacc
     1801 tgccgcgcgg cctaccgcgc aaccggatgc cgcgactatg cccgcattga tctgcgcctg
     1861 cgcgacggca tcttcaacgt gctggacgtg aatcccaatc ccgacatcag ctccgacgcc
     1921 agcatggcct gcgcggcgga cacccttgga atttcctacg gcgaactcgg ccgccggatt
     1981 gtcgaaatgg cccttgagcg ccatccggtc ctgccggccc gcaaaccgcg cccggcaaat
     2041 gctcccgtct ctgcggacgc ggtcgcaagc cccggatgaa aacggttgat ggttaaaggt
     2101 tgcaggttca aggtttccag tctcaagcat caagcatcca gtatccgctt cctagcagtc
     2161 taaaggacta cagcctaaaa gacttcttcc ctgaattcat atgagtgcag tccgcacccc
     2221 gcacattcgc cgccaatttt cattgacaag acgtatagtt tatatatatt tatacgtcat
     2281 gaatacaaaa ctcacattgc gcatggatga gtcgctggtt gaaaccgcca aagaagaggc
     2341 ggccagccgc ggcaagtcgg tttctcaaat ggtcggtgat tttttcggct cacttaaaag
     2401 caccgctcac agcaaacgca accgacctcc aattacagca tctctgctcg gtgttctccg
     2461 caaccgcaaa ttgtcggagg cggattacaa aaagcatctg cgggagaaat atcggtgagg
     2521 atcattgttg atacaaacgt ggttcttgat gtgctgctgg accggaagcc gtttgcgaac
     2581 gccgccgccg gggtattcag tttgattgaa gaatcaaaat ttgaaggcat gctgtgcgcc
     2641 acaaccgtca caaccattga ctatctcctc tcgcaggcgt tctcccgccc gaagagtctc
     2701 gagacgctgc gccgcctgct tgaactgttt gaaatcgctc cggttaatcg cccggtcatt
     2761 gaagaagcct ttcgaagcaa aataacggat tttgaggacg cggtgctgga acaggccggc
     2821 cgcctcgccg gcgcggatgc cattgtgacc cggaatacac gcgacttccg gaattcggcc
     2881 atcaaggcca ttgggccaga tgaactgctc tctgccttca ttgaataata cgacgacgct
     2941 cgggtgatct gaattgactt ccattacacg ggggcatagc gtcaggatca gataacatat
     3001 tggagagttc tatgccgcat cagtctgaaa caaaaaacat cgccgtgttc tgcgatttcg
     3061 agaacgtcgc gctcggcgtg gcggacgcga agtacgccaa gttcgacatc atgaaggtgc
     3121 tcgagcgcct gctgctgaag ggcaatatcg tcgtcaagaa ggcctactgc gactggggcc
     3181 gctaccgcga cttccgcccc gccatgcacg aggcatcctt cgagcttatc gaaatccccc
     3241 atgtccgcat gtccggcaag aactccgccg acatccgcat ggtcgtggac gccctcgacc
     3301 tctgttacac caaggagcat gtggacacgt ttgtggtcat cagcggcgac tcggacttct
     3361 ccccgctggt cagcaagctg cgcgagaaca acaagaccgt tgtcggcgtc ggcgtgaaga
     3421 attcaacctc cgacctgctc atcgccaact gcgacgagtt cattttttat gacgatctgg
     3481 tgcgggcgca gccgaaacct gcaaggccgc gcgcggcggc atcaaaatcc gcaaagaccc
     3541 cggcaaaggc ggccgcttca accgatgata aaaagctgga ggccttcaac ctcatcgttg
     3601 cgacttatga ggcgctcgtc gaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnaaaaag ctggaggcct tcaacctcat cgttgcgact tatgaggcgc tcgtcgaaga
     3781 gcgcggcgag gaggaaaaca tctggggctc catgatcaag cagacgctca agcgccgcaa
     3841 tcccggtttc agcgaatcct tctacggctt ccgctccttc ggccagcttc tggaggaggc
     3901 gggccagcag ggctttctcg aactcgagcg cgatgaaaaa tccggcggct acatcatcaa
     3961 gggctgccgg caggcgtagg cagaagacag aattcagaag gcgaagaaag cgctgcggca
     4021 tcattccatc attcatcttt aaccccgtcc ccctgtgccc ttgtgcctct gtgcccgtct
     4081 tcctctgcgc gggacttccg gaatattcac cacaaaagag cacaaaggtc gcaaagaatt
     4141 ttgagtcttc tgtacctttt cgcggccagc attctgaatc ctgccgaacc ggtcagcgct
     4201 gaaaacccgg agcgtgacaa ggcggaccgg atatgataga ttactggcag tggtaatgga
     4261 gaacgtgata agcatgagtg aaatagtgtt tgaggttaca caggaagcgg atggcggatt
     4321 tgttgcggaa tgcctgaatg agagcatctt cacacaggcc gacacgtggg atgagcttcg
     4381 caggaatgtc caggacgcga tttctgcctt ctattttgat cgccctcatc cttctgtggt
     4441 ccgacttcat cttgtgcgcg acgagatcct ggcggctgta tgaaactgcc gcgcgacctg
     4501 agcggtacag atttggttca ggcattgtcc cggtcatggg cttaccagaa ggttcatcag
     4561 tcagggagcc atatcatact gcaaacagaa acgccctttc ctcatcgcat tgcagttccg
     4621 gcccatgccg ttttgcggct gggcaccctg aatgcgattc tgcgtgcagt cgctgcacat
     4681 aaaggggttg gccgggaaga tatcctgaag tccttgtaat ccgacggcag catcagcgca
     4741 ggcagaagtc agaaggcgca gaaagcgctg catcattcca ccgttcctgt ctttctgtcc
     4801 cttttctccg cgtctctgcg tctctgcgcg acaatttctt cattctgaat tctggctcct
     4861 gaaccctgct cctccgcgct gccgcggtca ccgcccgcgg ctacagcttt gcgccctgca
     4921 gcttttcggc cttcgcctcg gcctcgtcgg cttccgcggc gcggcccgcc tgccgcagga
     4981 agaacgcata ttcacgccag agcatcggat gatcaggcag caggccaacc gcatattcat
     5041 agtccgcgca ggcggccgcc ggatcgccca gcgtcccgcg atattttccg cgcagggcgc
     5101 ggaacatcgg agcatccgga tacgccgcca tcccctgctc cagaaactgc cccgccagat
     5161 cgggctggtt ggactgcatg gccagaatca ccatttgata aagagcccag aactcgtcgg
     5221 ggctgtactg caaaacggcc tgatactgat accacgcctc ctggaaattc ctctgcttgt
     5281 tcagctcatt gg
//
LOCUS       DAOQWR010000158         5287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_15891_length_5287_cov_5.176988, whole genome shotgun sequence.
ACCESSION   DAOQWR010000158 DAOQWR010000000
VERSION     DAOQWR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15891_length_5287_cov_5.176988"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..686
                     /locus_tag="PKK36_10510"
     CDS             228..686
                     /locus_tag="PKK36_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNR95023.1"
                     /translation="MTQDFKWYINPVFWCALIAWLVAQGTKMLTHFVRTREVDFHYLV
                     STGGVPSAHSAMAAALATSVGLQVGFSEPLFAVTLAFAIVVMFDAQSVRRAAGQQAKL
                     LNQIVAEIFKEHHLSQQKLAELLGHTPLEVFLGLLMGVLTALIVLSRFGM"
     gene            692..1585
                     /gene="metF"
                     /locus_tag="PKK36_10515"
     CDS             692..1585
                     /gene="metF"
                     /locus_tag="PKK36_10515"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNR95024.1"
                     /translation="MADERISRLIEDRKKPLLSFEFFPPKDDDSFAALEQTAGRLCKL
                     KPDFITVTYGAGGSTRDRTLKMALRLREMYSVPVMPHLTCVGATVGELNEIIRTMYES
                     GFRSIMALRGDPPRDQPAFVAPEGGLSHASELVTLIKNEFPDMGCAVAGYPERHPEAP
                     SLEADIDHLKEKIDCGADFVTTQLFFENADFQWYRDMCRARGVTAPILPGLLPVISLP
                     QARRIMELSQVAFPAALDQKLRRAGEKGPEAEKEGIRWAVDQIEELIESGVPGIHLYI
                     LNRPKTALDSALEDCIHRWHG"
     gene            1677..2414
                     /locus_tag="PKK36_10520"
     CDS             1677..2414
                     /locus_tag="PKK36_10520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR95025.1"
                     /translation="MNDTCHLYTDLAWLWPMWGDAATEYAHYCQYVTGLIRQHSKLPA
                     DTLLDIGCGGGKNVLNLKRDFRVTGLDLSPAMLAQAKELNPGCTFVEGDMRTCRLGRT
                     FDAVLMDDAISHMNCRADFEAAFRTAHAHLNPGGVLVATPDVTIETFCQNKTTTTPAA
                     RDGLDVVFVENVYDPDPADEQYETTILYMIRDHGRLRIETDHWTMGIFSLETWRQVLR
                     ATGFEVHEGRYHQDEDEYTVFTCVRPV"
     gene            2905..5034
                     /locus_tag="PKK36_10525"
     CDS             2905..5034
                     /locus_tag="PKK36_10525"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNR95026.1"
                     /translation="METTKTKTQGDELLSAITTIRKRDGRCMPFNASKITSAIAKAGR
                     ETGEFGDDVARKMTMRVISLYQAMFPGEAPEVEKIQDLVEEVLLASAYKKTAKAYILY
                     RDQHARIREMSKKADLDLIDSYLEQLDWKVHENSNMAYSLQGLNNFISSEISKTYWLN
                     KIYTPEIREAHDTGAFHIHDLGQLSVYCVGWDLKDLLVSGFRGAYGKTESSPAKHLCT
                     ALGQIVNFFYTLQGEAAGAQAFSNFDTLLAPFIRYDKLDYQEVKQELQEFVFNVNVPT
                     RVGFQSPFTNVTLDLQPPSAMAGENVIIGGRPQKETYGEFQAEMNMFNTAFMEVLAEG
                     DAKGRVFTFPIPTYNITADFNWDAPELARLWEVTAKYGIPYFANFVNSDMSPDDARSM
                     CCRLRLDTRALEKRGGGLFGANPLTGSIGVVTINMPRLGHLAENAADFMMRLDHLMEV
                     ARASLETKRKVLETFTDKKLYPYTSFYLRQIRQRHGCYWFNHFSTIGLVGLNEAVVNL
                     MGKDIGTPEGKDFAARVLTFMRDKLAEFQQTTGNFYNLEATPAESTGYRLARLDKKLY
                     PDIICANETETDEPFYSNSSQLPVNYSDDIFHVLDLQDELQTKYTGGTVQHVFLGEAV
                     ADPEAVKNFVRTVCHNYHLPYFTLSPTFSVCPSHGYLKGEHKTCPHCGETAETYSRVV
                     GYLRPVDQWNKGKRAEFGLRARYHVEA"
     gene            5037..5261
                     /locus_tag="PKK36_10530"
     CDS             5037..5261
                     /locus_tag="PKK36_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR95027.1"
                     /translation="MRIGGFMPFTLSDYPGLPAAVVFTQGCNFRCPFCHNGDLLSAVR
                     PQSQLIPEETVFELLKKRGGRLQGVVITGG"
BASE COUNT         1118 a         1616 c         1507 g         1046 t
ORIGIN      
        1 ccatcagccc cgaactgccc gcgatgcatc cgccggagcc gcaggtccgg tcggtggccg
       61 cggcgccgtc gccgtcgttt cccggcccgt tcctggccca ctcaaatgcc gaaccctccg
      121 gcattcagta cgggccgctg ccgtcgagcc ttgtgaattt caattattaa gttcttccgt
      181 tggacgggcg gtatgcctta atctgcgggg atgttgggaa accgctcatg acacaggatt
      241 ttaaatggta cattaatccc gttttctggt gcgcgctgat cgcgtggctg gtggcgcagg
      301 gcacaaaaat gctgacgcat ttcgtcagaa cccgggaagt tgacttccac tatctcgttt
      361 caaccggcgg tgtgcccagc gcgcattccg ccatggccgc cgcgctggcg acctccgtgg
      421 ggctgcaggt cggtttctcc gagccgctct tcgcggtcac cctcgccttc gccattgtcg
      481 tgatgttcga cgcgcagagc gtccgccgcg ccgcgggcca gcaggccaag ctcctcaacc
      541 agatcgtcgc ggaaattttc aaggagcatc atctttcgca gcagaagctc gccgaactgc
      601 tcggccatac gccgctggag gttttcctcg gcctgctgat gggcgtgctg acggcgctga
      661 ttgttctctc cagattcggg atgtgatgat catggcggat gagcgaataa gccggctgat
      721 cgaggacagg aagaaacccc tgctgtcctt cgaattcttt cccccgaagg acgatgacag
      781 cttcgcggcg ctcgagcaga ccgccggccg gctctgcaag ctgaagcccg atttcatcac
      841 cgtgacctac ggcgcgggcg gctccacgcg cgaccgcacc ctcaaaatgg cgctgcgcct
      901 gcgcgaaatg tacagcgtcc ccgtcatgcc tcatcttacc tgcgtgggag cgacagtcgg
      961 ggagttgaac gaaatcatca ggaccatgta cgaaagcggc ttccgcagca tcatggccct
     1021 gcgcggcgat ccgccgcggg accagccggc cttcgtcgcc cccgaaggcg gactgagtca
     1081 cgcgtccgaa ctcgtcaccc tcatcaaaaa tgaatttccg gacatgggct gcgccgtcgc
     1141 cggctatccc gaaaggcatc ccgaggcgcc ctcgcttgaa gctgacattg atcacctgaa
     1201 agagaaaatt gactgcggcg cggacttcgt caccacgcag ctcttttttg aaaatgcgga
     1261 cttccagtgg taccgcgaca tgtgccgcgc gcggggcgtc accgcgccca ttttgcccgg
     1321 cctgctcccc gtgatatcgc ttccgcaggc aaggcggatc atggagcttt cgcaggtggc
     1381 tttcccggcc gcgctcgatc agaaactcag gcgggcgggg gagaagggcc ccgaggcgga
     1441 aaaagagggc attcgctggg cggtggacca gatcgaagaa ctcatcgaat ccggcgtgcc
     1501 cggcatccat ctctatatac tgaaccggcc caaaaccgcg ctcgattccg cgcttgaaga
     1561 ctgcattcac cgctggcacg gctgaaccgg cccgaaatca gccctttttg ctcaacttta
     1621 tattctgaat ctggtaacag aaaacctgca gtcatgtgtt gggcatcata cgagaaatga
     1681 acgatacctg tcatctttac accgacctgg cctggctgtg gccgatgtgg ggcgatgccg
     1741 cgacggagta cgcgcactac tgccaatacg tcaccggcct gattcgccag cactccaaac
     1801 tcccggcaga cacattactg gacatcgggt gcgggggcgg gaagaacgtc ctgaacctga
     1861 agcgagactt cagggtcacg ggtctggatt tgagtcccgc gatgctcgcg caggcgaagg
     1921 aactgaatcc gggatgcacg tttgtcgagg gcgatatgcg gacatgcaga ctcggccgga
     1981 ccttcgacgc cgtgctgatg gacgatgcta tttcgcacat gaactgccgg gccgatttcg
     2041 aggcggcatt ccgcaccgcg catgcgcact tgaatccggg cggcgtcctt gttgccacgc
     2101 cagacgtgac catcgagacc ttctgccaga acaagaccac gaccacaccg gccgcgcggg
     2161 acggattgga cgttgttttt gtagagaacg tgtatgaccc cgatcctgcg gatgagcagt
     2221 atgaaacgac aatcctctat atgattcgtg atcatggccg cctgaggatt gagacggacc
     2281 attggacgat ggggatattc tctctggaaa catggcggca ggttttgcgc gcaacaggtt
     2341 tcgaggtgca tgaggggcgg tatcatcagg atgaggatga atacactgta ttcacgtgcg
     2401 tcaggccggt atgagggccg gaccatgtcc tgcgcgtttt tccgcaaaaa tcattggatt
     2461 tttagcagaa tcgggcgctc ggggccggtt ttttgccgaa aaaagcgaaa aaatgacgaa
     2521 aaatcgctga aaatcttcaa aaaacggcaa aaaacttcaa aaaatggtca aaaacggacc
     2581 gaaaacagcg aaaatctgca ttttttcgta aaaaaatgcg aaaaatgata aaaactggcc
     2641 cgcaaaactt ccgcgggtca ttttttttgc ggggtgcccg gcgaggctga aaaagtgcga
     2701 tttcggccgt ttttcctcat ttcggagcct ctgcgggtcg taatcgtctg aaaatgaacg
     2761 aattgtaaat ttcacatgat tttgtgttga ctcatttttt gctccactat atgttgtgtt
     2821 tttcaatcaa gccctgttaa taactttttt cgggcttgtt ctggggagac ggtttttttg
     2881 agaagcaaat agtgaggatg aatcatggaa acaaccaaga caaaaacaca gggagatgaa
     2941 cttctttccg ccatcacgac cattcgcaag cgcgatggcc gctgcatgcc tttcaacgcg
     3001 tccaagatca cgtcggccat cgcgaaggcc ggccgcgaaa ccggagagtt cggcgacgat
     3061 gtcgcgcgca agatgacgat gcgcgtgatc agtctctatc aggcgatgtt ccccggcgaa
     3121 gcgcccgagg tcgagaagat ccaggacctc gttgaagagg tgctgctcgc ctccgcctac
     3181 aagaagaccg ccaaggccta cattctctat cgcgaccagc atgcgcgcat ccgcgagatg
     3241 tccaaaaagg ccgatctcga tctcattgac agctatctcg agcagctcga ctggaaggtg
     3301 cacgagaaca gcaacatggc ctattcgctc cagggcctga acaatttcat ctccagcgaa
     3361 atcagcaaga cctactggct gaacaaaatc tacacacccg aaatccgcga ggcgcacgac
     3421 accggcgcgt tccacatcca tgatctcggc cagctctccg tctactgcgt cggctgggac
     3481 ctgaaggacc tgctggtctc cggtttccgc ggcgcctacg gcaagaccga aagctcgcct
     3541 gcgaagcatc tctgcaccgc cctcggacag atcgtgaatt tcttctacac gcttcagggc
     3601 gaggccgccg gcgcgcaggc gttctcgaat ttcgacacgc ttctggctcc gttcatccgc
     3661 tatgacaagc tggactatca ggaggtgaag caggagctgc aggagtttgt cttcaacgtc
     3721 aacgtgccca cgcgcgtcgg ctttcagtcg cccttcacca atgtgacgct tgaccttcag
     3781 cctccgtccg cgatggccgg cgagaacgtg atcatcggcg gcaggccgca gaaggaaacc
     3841 tacggcgagt tccaggccga gatgaacatg ttcaacaccg ctttcatgga ggtgctggcc
     3901 gagggcgacg cgaagggccg cgtgttcacg ttcccaatcc cgacctacaa catcaccgcc
     3961 gacttcaact gggacgcgcc cgaactggcg cggctctggg aagtcaccgc gaaatacggc
     4021 attccctact tcgcgaattt cgtcaattcc gacatgtcgc ccgacgacgc ccgctcgatg
     4081 tgctgccgcc tgcggctgga cacccgcgcg ctggaaaagc gcggcggcgg cctcttcggc
     4141 gccaatccgc tgaccggctc catcggcgtg gtcaccatca acatgccgcg cctcgggcat
     4201 ctggccgaga acgccgcgga cttcatgatg cggctggatc acctgatgga agtcgcccgc
     4261 gccagccttg aaaccaagcg caaggtgctc gagaccttca ccgacaagaa gctttatccg
     4321 tacaccagtt tttatctgcg ccagatccgc cagcgtcacg gatgctactg gttcaaccac
     4381 ttctcgacca tcggcctcgt gggcctgaac gaggccgtcg tgaacctgat ggggaaggac
     4441 attggaaccc cggaaggcaa ggacttcgcc gcgcgcgtgc tgaccttcat gcgcgacaag
     4501 ctggccgaat tccagcagac caccggaaac ttctacaacc tggaggccac cccggccgaa
     4561 agcaccggct accgcctcgc gcggctcgac aagaagctct atcccgacat catctgcgcc
     4621 aacgaaacgg agaccgatga gccgttctat tccaactcga gccagctccc ggtgaactac
     4681 agcgacgaca tcttccacgt gctggacctg caggacgagc tgcagaccaa gtacaccggc
     4741 ggaaccgtgc agcacgtgtt cctcggcgag gccgtggccg atccggaggc cgtcaagaat
     4801 ttcgtccgca cggtctgcca caactatcac ctgccgtatt tcacgctttc gccgaccttc
     4861 tcggtgtgcc cttcacacgg ctatctgaag ggcgagcaca agacgtgccc gcactgcggc
     4921 gagacggcgg aaacctactc gcgcgtggtc ggctatctcc ggccggtgga ccagtggaac
     4981 aagggcaagc gcgccgagtt cggtctgcgc gcgcgctatc acgtggaggc ctgaggatgc
     5041 ggatcggcgg gttcatgcca ttcacgctct cggactaccc cggcctgccg gccgcggtgg
     5101 tcttcacgca gggatgcaac ttccgctgcc cgttctgcca caacggcgac ctgctttccg
     5161 ccgtgcgccc ccaaagccag ctcatcccgg aagagaccgt ctttgagctg ctgaaaaagc
     5221 gcggcggccg tctgcagggt gtggtcatca ccggcgggta agcggatcgc atcggcctcc
     5281 agcagcc
//
LOCUS       DAOQWR010000159         5163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_16373_length_5163_cov_4.458888, whole genome shotgun sequence.
ACCESSION   DAOQWR010000159 DAOQWR010000000
VERSION     DAOQWR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16373_length_5163_cov_4.458888"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="udk"
                     /locus_tag="PKK36_10535"
     CDS             <1..610
                     /gene="udk"
                     /locus_tag="PKK36_10535"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNR95028.1"
                     /translation="GGTASGKTTVARKILEQVGAGRVAYLPHDAYYRDMSHLPVAERS
                     RMNYDHPNALETSLLVTHIRELLEGRAVQLPVYSFADYCRKEETVAVEPAPVILVDGI
                     LILAEPDLRALMDIKLFVDTDADVRLIRRMERDIRERGRSLDSTVQQYMETVRPMDLE
                     FVQPSRRYGDVIIPGGGMNRVAIDMVVLKLRDILQQHGVDSK"
     gene            607..1626
                     /locus_tag="PKK36_10540"
     CDS             607..1626
                     /locus_tag="PKK36_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HNR95029.1"
                     /translation="MSLNAGKIDQAALERILAYTGTADPQVLLGPRFGEDAGVVRLGP
                     GGLVAASDPVTLASEQVGWYAVHINANDIAVTGGIPRWFLSTILLPPSTADGVLERIF
                     LQIHDACRELAVAVIGGHTEVSMSVRQPLVCGTMLGTVDPDRIIRTAGARTGDVIIMT
                     KGVCIEGAALLALDRPVESKFILGEERWKKVCNYLREPGISVLRDARIACETAEVHAM
                     HDPTEGGLNMGLYEMAVSSGLGVELDEDSILFIDDSVTLCERFAMDPLRTLTSGTLLI
                     AVAPAEADSLLAAYHRKNIPAAVIGRMVPEGFTVFRRGLRYPLVCSAQDETVSLINRE
                     LFPEP"
     gene            1644..2057
                     /locus_tag="PKK36_10545"
     CDS             1644..2057
                     /locus_tag="PKK36_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95030.1"
                     /translation="MMLPDLQSSILCDDVRQERNGKFMLIGLFDAIAAHQLPVRYPRL
                     FMVTRWCSGEGSFIQQTRILKPDQRTLLIEGKHIPVKLPAEDTTATNVEVFVNVEFAE
                     AGTHWIEIHLDNTLKIRYPLRVSIVPHPVPPPQAS"
     gene            complement(2066..2773)
                     /locus_tag="PKK36_10550"
     CDS             complement(2066..2773)
                     /locus_tag="PKK36_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95031.1"
                     /translation="MCAFSLFRKTLPGTVITVEDLSGAIGTAIDQAFKNYIPQPDKMA
                     STVKSALEDAGRDLSSQLQAGAKEFGTQVAAFKAAAEKLESALKTHVEKMSSAVTEAS
                     KGAGDVLADKLKAGAKSFETSLGSLDSSAGKIDSALKGHTTELQKTLAESSKQMEAAG
                     AAVAEKLGEVTRLQQQIDSVLQLQKTVEGTIKSIAASSDFTSTLSALQKHLAESDNLV
                     KMASKPRTIRLVENPAE"
     gene            2866..3549
                     /locus_tag="PKK36_10555"
     CDS             2866..3549
                     /locus_tag="PKK36_10555"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR95032.1"
                     /translation="MATAKTALLMGGMMLLYGCGGPQNAVRIGHRKHVLCPVPAPAGG
                     AAFLMGSLEISVAEYAAYLNENPPAKQHPQIVCAPKGCRPSRGLDKHPAAHVSRTEAE
                     NFCYWLSRKTGRTVRLPTAAEWEHAARGGINGSDYPWGWGSPSSNACFRTEGPVECGQ
                     YDPNPYGLHDMAGNVAEWCSDNAGSQLVALGGSWAERSEELLRVDRCTLFPPDYSDAD
                     VGFRILVER"
     gene            complement(3605..4546)
                     /gene="cysK"
                     /locus_tag="PKK36_10560"
     CDS             complement(3605..4546)
                     /gene="cysK"
                     /locus_tag="PKK36_10560"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNR95033.1"
                     /translation="MNYYTDNSLSIGNTPLVQIRKLAGSKALVLGKIEGRNPAYSVKC
                     RIGASMVWDAEKKGILKPGMEILEPTSGNTGIALAYVGAARGYGVTLTMPETMSIERR
                     KILDAFGAIVVLTEGAKGMKGAIEKADQIMAEYPGQFYRPQQFKNPANPAIHEKTTGP
                     EIWKATGGNIDVLVAGVGTGGTISGIARYFKKTMDRNVLTVAVEPEASPVISQAMAGD
                     PLQPGPHPIQGIGAGFIPDTLDLSVIDRVEKVGGDEAMEYARRLAKDEGILSGISCGA
                     ALAAAARLARKREFEGKTIVVILPDSGERYLSTSLFA"
     gene            complement(4543..>5163)
                     /locus_tag="PKK36_10565"
     CDS             complement(4543..>5163)
                     /locus_tag="PKK36_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNR95034.1"
                     /translation="LGLVDKDAVDLSNLQRQILHSSEDLGRLKVHSGSESISALNPDV
                     HVVTYHMRFTADNAMDLLKDYDFIIDATDNLASKFLIADASFLAGKPYSHAGILEYYG
                     QTLTVLPGETACYRCIFRSEPAECPPFSKGVLGAVPGVVGTIQATEAIKHILGIGRLL
                     TNRLLTYDALDMVIRTIDIHRNADCPLCGKNPEIRDLRRHPSGGKQ"
BASE COUNT          962 a         1529 c         1596 g         1076 t
ORIGIN      
        1 cgggggcact gcgtccggaa aaaccaccgt ggccagaaag attcttgaac aggtgggcgc
       61 cggtcgcgtg gcctatctgc cgcacgacgc ctattaccgc gacatgtcgc accttccggt
      121 ggcagagcgg tcccggatga actacgatca tcccaacgcg ctcgagacca gcctgctggt
      181 gactcatatc cgggaactgc tggagggccg cgccgttcag ctgccggtct acagcttcgc
      241 ggactactgc cggaaggagg agacggtcgc cgtggagccg gcccccgtca tactggtgga
      301 cggcattctt attctcgccg agccggacct ccgcgcgctg atggacatca agcttttcgt
      361 ggacacagat gcggatgtgc gcctcatccg ccgcatggag cgcgacatcc gggagcgggg
      421 acggagtctg gactcgacgg tgcagcagta tatggagacg gtgcgcccca tggatcttga
      481 atttgtgcag cccagccgcc ggtatggcga tgtgatcatc ccgggcggcg gcatgaaccg
      541 cgtggcgatt gatatggtgg tccttaaact ccgggatatt ctgcagcagc atggagtgga
      601 ttcgaaatga gcctgaacgc gggaaaaatt gatcaggcgg cgctggaacg aatcctggcc
      661 tataccggca cagccgatcc ccaggtgctg ctgggacccc ggtttggaga ggatgccggc
      721 gtggtgcgcc tgggcccagg cgggctggtg gccgccagcg atccggtgac actggcttcg
      781 gagcaggtcg gctggtatgc cgtgcatatt aatgcgaatg atattgcggt gaccggagga
      841 attccccgct ggtttctttc gaccatcctg cttcccccca gcacggcgga cggcgttctg
      901 gagcgcatat tcctgcagat ccacgatgcc tgccgcgaac tggccgtcgc ggtgattggc
      961 gggcacacgg aagtttcgat gagcgtgcgc cagccgctgg tgtgcggcac catgctgggc
     1021 acggtggatc cggatcggat catacgcacc gccggcgcgc gcacgggcga tgtcatcatc
     1081 atgaccaagg gcgtgtgcat cgagggcgcc gctctgctgg cgctggaccg cccggtggaa
     1141 tcgaagttta ttctcgggga ggagcgctgg aaaaaagtct gcaactacct gcgtgagccc
     1201 ggcatcagtg tcctccggga tgcccgcatc gcctgtgaaa ccgctgaagt tcacgccatg
     1261 cacgatccga ccgagggcgg tctgaacatg gggctctacg agatggccgt ttcgtccggc
     1321 ctgggggtgg agcttgacga ggacagcatt ctgttcattg acgattcggt gacgctgtgt
     1381 gaacgctttg ccatggatcc gctgcggact ctcaccagcg gcaccctgct gattgcggtg
     1441 gcgcccgctg aggcggacag cctgcttgcg gcctatcaca gaaaaaacat cccggcggcc
     1501 gtgatcgggc gcatggtgcc ggaggggttc actgtgttca gaaggggcct gcgctatccg
     1561 ctggtctgca gcgcacagga tgaaaccgtc agcctgatca accgcgaact gttccctgaa
     1621 ccatgagcga aggagaattc aaaatgatgc ttccggatct gcagtcaagc attctgtgcg
     1681 acgatgtccg ccaggaacgg aacgggaagt tcatgctgat aggcctcttc gatgccattg
     1741 cggcccacca gctcccggtg cgctatcccc gcctcttcat ggtcacccgc tggtgcagcg
     1801 gggagggcag ttttatccag cagacccgca ttctcaagcc tgaccagagg accctcctca
     1861 tcgagggcaa gcatatcccc gtaaaacttc cggcggaaga caccacggca accaacgtgg
     1921 aggtttttgt gaacgtggag tttgccgagg ccggaaccca ctggatcgaa atccacctcg
     1981 ataacaccct gaaaatccgc tatccgcttc gcgtcagcat cgtccctcat ccggtgccgc
     2041 cgccgcaggc ttcgtgatcc gcggtctatt ctgccggatt ttcgaccaga cggatggtgc
     2101 ggggcttgga cgccattttc acgaggttgt cagattcggc cagatgcttc tgcagcgcgc
     2161 tgagtgtgct cgtgaagtcg ctggacgccg cgatggattt gatggtgccc tccacggttt
     2221 tctgaagctg gagaacgctg tcaatctgct gctgaagcct ggtaacctcg cccagttttt
     2281 ccgcgacggc ggcgccggcg gcttccatct gcttgctgct ttcggcaaga gtcttctgaa
     2341 gctctgttgt atggcccttc agggcgctgt caatctttcc ggcgctgctg tccagcgagc
     2401 ccagactggt ttcaaaagac ttggcgccgg ctttcagctt gtcggccagg acatccccgg
     2461 cccccttgga ggcctccgtg acggccgatg acattttttc gacatgagtc ttcagggcgg
     2521 attccagttt ctcggcggcg gccttgaaag ccgcaacctg cgtgccgaat tccttcgccc
     2581 cggcctgcag ctgggaggac agatccctgc cggcatcttc gagcgccgat ttcacggtcg
     2641 aggccatctt gtccggctgg gggatgtagt tcttgaaggc ctggtcaatg gctgtgccga
     2701 tggctcccga gagatcctca acagtgatga cggttccggg caacgttttc ctgaacagtg
     2761 aaaatgcgca catagtgcct cctgcttata agctgtttac ctttccaaca gccgtgatag
     2821 tatacaaacc gtccggtcat ggtaagcata aaaacggaga aaatcgtggc gacggcaaaa
     2881 acagcgctgc tgatgggggg gatgatgctg ctgtacggat gcggcggccc gcagaatgcg
     2941 gttcgcattg gacatcggaa gcacgtgctt tgtcctgtgc ctgccccggc cggcggcgcc
     3001 gcgtttctga tgggaagcct cgaaatctcg gttgccgaat acgcggctta cctcaatgag
     3061 aatcccccgg cgaagcagca cccccaaatc gtctgcgccc caaaaggctg caggcccagc
     3121 cgcgggctgg acaagcatcc tgccgctcat gtttcacgaa ctgaagcgga gaatttctgc
     3181 tactggctgt cacggaaaac aggccgcacc gtgcggctgc caaccgccgc tgaatgggag
     3241 catgccgccc gcggaggaat caacgggtcg gactatccct ggggctgggg cagtccgtcc
     3301 agcaacgcct gcttcaggac cgaaggcccg gttgaatgcg ggcagtatga tccgaatccg
     3361 tacggcctcc atgacatggc cggcaacgtg gccgaatggt gctctgacaa cgcaggcagt
     3421 cagctggtgg ctcttggcgg aagctgggcc gagcggagcg aggaactcct gcgcgttgac
     3481 cgatgcacgc tcttcccccc ggactacagc gatgcggacg tcggcttccg gattctggtc
     3541 gagagatgat gtctccggcg ggacagttct ccctccggca cccggagaaa caagcgcgcc
     3601 ggcttcatgc aaacagcgat gttgacaggt accgctcgcc ggagtcgggc agaatgacca
     3661 cgatggtctt gccttcaaat tcccgtttgc gcgcaaggcg agcagccgcc gccagagccg
     3721 cgccgcagga gatgccggaa agaataccct catctttcgc cagacgcctt gcgtattcca
     3781 tagcctcgtc tccgccgact ttttccaccc ggtcaatgac cgaaaggtcc agcgtgtcgg
     3841 ggatgaatcc tgcgccgatg ccctgaatcg gatgagggcc gggctggagc ggatcgcccg
     3901 ccatggcctg cgagatgacg ggactggctt cgggttcgac tgcaacggtc aggacattcc
     3961 tgtccatggt cttcttgaaa tagcgggcaa tgccgctgat ggttcctccc gtgccgacac
     4021 cggccaccag cacatcgata ttccctccag tggccttcca gatttccggt ccggttgttt
     4081 tttcatggat ggcggggttg gcggggtttt tgaactgctg gggcctatag aactgcccgg
     4141 ggtattccgc catgatctgg tcagctttct caatggcgcc cttcatgccc ttcgcgcctt
     4201 cagtcagcac cacgatggcg ccgaaggcat ccagaatttt gcggcgctca atgctcatgg
     4261 tttcgggcat ggtcagcgtg acgccatagc ctcgcgcggc gccgacgtag gcgagggcaa
     4321 tgcccgtgtt gccgctggtg ggttccagta tttccatgcc gggcttcaga attccttttt
     4381 tctcggcgtc ccagaccata gaggccccga tgcggcattt gaccgaataa gcgggattgc
     4441 ggccctcaat tttgcccagc acaagcgcct tgctcccggc cagttttcga atctgcacga
     4501 gcggagtatt cccgatgctc agcgagttgt ccgtatagta gttcattgtt tacctcctga
     4561 tgggtgtctc ctcaaatccc tgatttccgg attcttcccg cacagcgggc agtcggcatt
     4621 tcgatggata tcaatggtcc ggatgaccat gtccagcgcg tcgtaagtca gcaggcggtt
     4681 ggtcagcagg cgcccaatgc ccaggatgtg tttaatggcc tccgtggcct ggattgtgcc
     4741 cacgaccccc ggcacggccc cgagcacgcc tttgctgaag ggcgggcact cggcgggttc
     4801 gctgcggaat atgcaccggt agcaggcggt ttctcccggc agcacggtca gggtctggcc
     4861 gtagtattcc agaatgcccg catgactgta gggcttcccg gcaaggaaag acgcatcggc
     4921 aatcaggaac ttgctggcga gattgtcggt tgcatctatg atgaagtcgt agtccttcag
     4981 caagtccatg gcattgtccg ccgtgaagcg catatgatat gtgacaacat gcacgtcggg
     5041 attgagcgcg gaaatcgact cgctccccga atgcactttc aggcggccaa ggtcttcgct
     5101 tgaatgcaga atctggcgct gcagattgct gaggtcaacg gcgtctttgt caacgagacc
     5161 cag
//
LOCUS       DAOQWR010000160         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_16637_length_5099_cov_3.600912, whole genome shotgun sequence.
ACCESSION   DAOQWR010000160 DAOQWR010000000
VERSION     DAOQWR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16637_length_5099_cov_3.600912"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKK36_10570"
     CDS             <1..329
                     /locus_tag="PKK36_10570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95035.1"
                     /translation="KQMIELMAESRAVVHFTEYEGFGMPPVEAVLAGTPPVYSQIPVA
                     QEVMGETGCPFSNEDYGSFSAAMNRALVAAPDEVQSWADALEQRHNWPQVANRVMDAL
                     RSFPAN"
     gene            378..4688
                     /locus_tag="PKK36_10575"
     CDS             378..4688
                     /locus_tag="PKK36_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR95036.1"
                     /translation="MKATAYIGLLVTAAACVCAPCARAADYYVAPDGSDAADGASLET
                     AWQSLPFAVHQITGGDTLYVRGGVYRGRTVFTNNGGSAEAPIRIYAYSNEVPVLKGSV
                     IVGEWEPFTNSIWRVTNWTHRSQQVFADEVLLQQIGWPNDFNRDRACSCSSWLYIPYG
                     YSCADIDPVTYTLNMGDPLTNMVPGSFYYCPTSQVLYVWLAGGESPTGRLMEVSRESG
                     IFYDWSDKGYLHVRGLAFRHSSTLTTASSGWPGVWAGYNGLIEDCDIEWCDGSGLGMR
                     NNSTVLNCRINNNGIQGIHANTHTNLSVIGCTIMSNNYRNYTLNHAAGIKFIINVSGT
                     VIASNEVAYNRARGIWFDTCPSNSRNVVAANYVHDNTPFPNSPGDPTSYANCGIYIEK
                     TGGVDVYNNIVVSNSTSGIQLSGASGCRVYNNYISGTRGTPGGHRGLAAVAVRPSGGV
                     SLPLKDNSIFNNIISDNLCDADLHLTDPNSINVERNYSDFNCIYRTAGAGIVFPSSHL
                     SVRTDPIKTDDLAVWSALTGWDTNSMNVTPLYDPGDGLASNSPCVDAGVTPDALAQYQ
                     RFGFGPLDGDGDGRAATDIGPCELQAEPAGTVYADALSTNAVFPYTNWNTAAAELADA
                     VAAAGDGGLVLLREGQYLLSEEVIITNAVTVRSAGCPADTRLIGTGADRCLTVTSQHA
                     LIDGLSIEQGSADQGGGVLMAAGLIQNCLVLSNYASAAGGGIYCASGAVVRNTTVIGN
                     ASSGDGGGLYLEQGAAALDCTVLQNGASGSGGGLYLEGAALVFGSLVHNNSSVDGGGL
                     YMAASAAVSRCTLRNNTASGKGGGVYCANGGLVSLSHVDGNRAAIGAGLYAAADSLFS
                     GCVVISNAASSAAGGVWMGGGGEVLHLTIAENAASNSAGGLFLDENAAVTASIIFNNR
                     APGSSNHVLSGGAMTYSCAAPLAAGSGNIDADPLFQQGFRLKYESPCVDGSASAGAGD
                     LDIDQNPRYVDGDGDGVSAADMGAYEYRNILYVDASSTNPVVPYRTRDTAAARIGDAL
                     LMSWHGDVIVVATGTYAITSQLLVNKGVSIYGEGRPDECVVQGNGKTRGWVLNHANAL
                     IRGFTITNCAADAGGGVYIKDAGTVQNCRITGNRATNDLGGAFQYFFGTFPVYCSITY
                     DSYVHEGGGGVVLARGGTLENCLIDNNEASRAGGVLCLDGGVLKNCTISANSASQQGG
                     GLVIQNTGAVQNAIIRFNEAPAGSNVFNAGSSYSYVYSCVAPLAAGEGNTDADPLFVS
                     SADFRLQPASPCIDAGDNSVTLNPLDLDGVPRVLDGDADGLAVVDMGAYEHVHGTNDA
                     DADGLSDILELQHGTDIFNRDTDDDGMTDGDEVVAGTNPTDANSVFSMSMESHVEPDK
                     MLIHWWSASNRIYDLMQSTNLLEGFAPHAVGLPATPPMNVYTDSITVDMKLYRVNVRE
                     "
     gene            4862..>5099
                     /locus_tag="PKK36_10580"
     CDS             4862..>5099
                     /locus_tag="PKK36_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR95037.1"
                     /translation="MKVGLKSVPECRYPAEPPFYPSESYPELLMRGRSAQVNAVYDAV
                     RETFHLLGYDASHRGTAAWNPLGWLIRPGETVFIK"
BASE COUNT         1034 a         1422 c         1604 g         1039 t
ORIGIN      
        1 cgaagcagat gatcgaactg atggccgaat cgcgcgcggt tgtgcatttc acggaatatg
       61 agggtttcgg gatgcccccc gtggaggccg ttctggcggg gaccccgccg gtttattcgc
      121 aaatcccggt tgcacaggag gtcatggggg aaaccggctg tccgttctcc aatgaagact
      181 atggttcctt ttccgcggct atgaaccggg cgctcgtcgc cgcgccggat gaagtgcagt
      241 cctgggccga tgcgctcgag cagcggcaca actggccgca ggtggcgaac cgcgtcatgg
      301 atgccttgcg cagctttccg gcaaattgag tgtatagtta accggcggaa ctgaatgttt
      361 cataaaaata gaaagccatg aaggcgacgg cttatatcgg gttgctggtg actgcagcgg
      421 cctgtgtctg tgcgccctgc gcgcgggcgg cggattacta tgtggcgccg gacggaagcg
      481 atgcggcgga tggagcatcg ctggagaccg cgtggcagag cctgccgttt gccgtgcatc
      541 aaatcactgg cggtgatacc ctctatgtgc gcgggggcgt ctatcgcggc cgcaccgtgt
      601 tcacaaacaa cggcgggtcg gcggaagcgc ccatccgcat ttacgcctat tccaacgagg
      661 tgcctgttct gaaggggtcg gttattgtcg gggagtggga gccgttcaca aacagcatct
      721 ggcgcgtcac caactggacg caccgaagcc agcaggtgtt tgcggatgaa gtgctgctgc
      781 agcagatcgg ctggccgaac gatttcaatc gcgaccgggc ctgctcgtgt tcttcgtggc
      841 tttacatccc gtacggctat tcctgcgcgg atattgatcc agtgacctac acgctgaaca
      901 tgggcgaccc gctgaccaat atggttccgg gcagcttcta ctactgtcca acctcgcagg
      961 tgctttatgt ctggctggcc ggaggggagt ccccgaccgg gcgtctgatg gaggtgagcc
     1021 gggagagcgg catattctat gactggtccg acaagggcta tttgcatgtc cgcgggcttg
     1081 cgttccgcca ttcgagcaca ctgaccaccg cttcttccgg ctggcccggc gtctgggccg
     1141 gctacaatgg attgatcgaa gactgcgata tcgagtggtg cgatgggagc gggctgggaa
     1201 tgagaaacaa ctcgacggtg ctcaactgcc gcatcaacaa caacgggatt cagggtatcc
     1261 acgccaatac gcacaccaat ctgtcggtga tcggctgcac gatcatgagc aacaactaca
     1321 ggaactacac gctcaatcat gcggccggga tcaagttcat cattaacgtc agcggcacgg
     1381 tgatcgccag caacgaagtg gcgtacaacc gcgcgcgcgg gatatggttt gacacctgcc
     1441 cctccaattc ccggaatgtt gtggcggcca attatgttca cgacaacacg ccgtttccca
     1501 attcgcccgg cgacccgacg agttatgcca attgcggcat ctatatcgag aagacgggcg
     1561 gggtggatgt gtacaacaat atagtggtca gcaacagcac cagcggcata cagctttcgg
     1621 gtgcttccgg ctgccgggtg tacaacaact acatctccgg aacgcgcggc acgccgggcg
     1681 ggcacagggg gcttgccgcc gtggccgtga ggccttccgg cggcgtcagt cttccgctca
     1741 aggacaacag catattcaac aacatcattt ccgacaattt gtgcgatgcc gaccttcatt
     1801 tgacggatcc caactccatc aacgtcgagc gcaactacag cgatttcaac tgcatctaca
     1861 ggacagcggg cgcgggcatt gttttcccgt cgagtcatct ttctgtgaga accgatccga
     1921 tcaaaacaga cgatctggcg gtctggtcgg ccctcaccgg ctgggacacc aacagcatga
     1981 atgtgacgcc gctgtatgat cccggagacg gactggcctc aaattctccc tgcgtggatg
     2041 cgggagtcac tccggatgcg ctggcgcagt accagcggtt cggattcggc ccgctggacg
     2101 gggacggcga cggccgcgcc gcaacggaca tcggcccctg tgagctgcag gcggagccgg
     2161 ccggcaccgt ttatgccgat gccctttcca ccaatgcggt gtttccatat accaactgga
     2221 acacggccgc cgcggaactg gcggacgccg tggccgccgc cggcgacggc gggctggttc
     2281 tgctgcgcga ggggcagtat ctgctttctg aagaagtcat catcaccaat gccgtcaccg
     2341 tgcggtcggc cgggtgtccc gcggacacgc ggctgattgg aaccggcgcg gaccggtgtc
     2401 tgacggtgac ctcgcagcat gcgctgattg acggtctgtc catcgagcag ggatcggccg
     2461 atcagggcgg cggggtgtta atggcggccg gactgattca gaactgcctg gtgctgtcga
     2521 attatgcatc cgcagccggc ggcggaatct actgcgcgtc cggcgcggtg gtcaggaata
     2581 caacggtgat tggaaatgcg tcatccggtg acggcggcgg cttgtatttg gagcaggggg
     2641 cggctgcgct cgattgcacg gttctccaga acggcgcttc gggctctggc ggcggcctgt
     2701 atttggaggg cgctgcgctt gtgtttggct ctctcgtgca taacaacagt tcagttgatg
     2761 gcggggggct ctatatggcg gcttccgccg ctgtgagccg ctgcacgctt cgaaacaata
     2821 cggcgtccgg aaagggcggc ggcgtgtatt gcgcgaacgg agggcttgtg agcctgtccc
     2881 atgtggacgg caaccgcgcg gccattggag cgggccttta tgccgctgca gacagtttgt
     2941 tttcgggctg cgtggtgatc agcaatgccg cctcgtccgc ggccggcgga gtctggatgg
     3001 gcggcggcgg agaggttttg catcttacaa tcgcggagaa tgcggcgtcg aattccgcgg
     3061 gcggactgtt tctggacgag aacgcggcgg tgacggcctc cattatcttc aacaaccggg
     3121 ctccggggtc ttcaaaccat gttctgtcgg gcggagccat gacctattcc tgcgccgcgc
     3181 cccttgcggc cgggagcggg aatattgatg ctgatcccct gtttcagcag ggcttccggc
     3241 tgaaatatga atctccctgt gtcgacggct ccgcatctgc gggtgccggg gatttggata
     3301 ttgatcagaa tccgcgatat gtggatggag acggggacgg cgtttcggcc gcagacatgg
     3361 gcgcttatga atacaggaac atactgtatg tggacgcgtc atcaaccaat ccggtggtgc
     3421 cctacagaac gagggacacg gccgcggcgc gcatcggcga tgcgctgctg atgagctggc
     3481 acggcgatgt gattgtggtt gcaaccggaa cctatgccat cacgtcccag ctgctggtga
     3541 acaagggcgt gagcatttac ggggaggggc gtcccgatga atgtgtcgtg cagggaaacg
     3601 gaaaaacgcg gggctgggtg ctgaatcatg ccaatgcgct gatcaggggt ttcacaatca
     3661 caaactgcgc ggctgatgcc ggcggggggg tgtatatcaa ggacgcaggc acggttcaaa
     3721 actgccgcat cacgggaaac agggccacca atgatctggg cggcgctttt cagtatttct
     3781 tcggcacctt ccccgtctac tgctccataa cctacgattc ctatgttcat gagggcggcg
     3841 gcggcgtggt gcttgcgcgc ggcggaacac tggagaactg cctgattgac aacaacgagg
     3901 cctcccgggc gggcggagtg ctctgcctgg atggaggagt tctgaagaac tgcaccatca
     3961 gcgccaacag cgcctcgcag cagggcgggg gtttggtcat ccagaacacc ggcgccgtcc
     4021 agaacgccat catccgcttc aatgaagcgc cggccgggtc caacgtgttc aatgccggca
     4081 gctcatacag ctatgtgtat tcctgcgtcg cgcccctcgc ggccggcgag gggaacacgg
     4141 acgcggatcc gctctttgta tcctccgccg acttccggct gcagccggcc tcaccctgca
     4201 tagacgcggg ggataacagc gtgacgctga acccgctcga tctggatgga gttccccgag
     4261 tgctggacgg cgatgccgac ggccttgccg ttgtggacat gggcgcctat gagcatgtcc
     4321 acggcacgaa tgacgccgat gccgacggtt tgtccgatat tcttgaactg cagcacggca
     4381 cggacatttt caaccgggat acagatgatg acggcatgac cgacggcgat gaagtggtgg
     4441 cgggaacaaa tccaaccgat gcaaattcag ttttcagcat gagcatggaa tcgcatgtcg
     4501 aaccagacaa aatgctgatc cactggtgga gcgcaagcaa ccggatatac gatctgatgc
     4561 agtcaaccaa ccttctggag ggcttcgctc cgcatgccgt cggcctgccc gccactccgc
     4621 cgatgaatgt ttatacggat tcgataacgg tggatatgaa gctctaccgg gtgaatgtcc
     4681 gcgaataatc cgcgaagact accaaaaaac atcagaaaat tcaagaaaaa ccgtaaaaaa
     4741 tcgctaaaaa acgcgaaaaa atgggcaaaa acgcgaaaaa atcatgaatt ttcatgaaaa
     4801 accgttattt tttgcgtatt tctcgtgatt ttctggattt tttgactgga aaccgatttt
     4861 tatgaaagtt ggactgaaat ccgtcccgga gtgccgatat ccggctgaac cgcccttcta
     4921 tccgtccgaa tcctacccgg aactactgat gcgcggaagg tcggcgcagg tcaatgcggt
     4981 ttatgatgcg gttcgcgaaa ctttccatct gcttggatac gacgcgtctc accggggcac
     5041 cgccgcatgg aatccgctgg gctggctgat caggccgggc gagacagtgt tcatcaaac
//
LOCUS       DAOQWR010000161         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_16807_length_5061_cov_5.032561, whole genome shotgun sequence.
ACCESSION   DAOQWR010000161 DAOQWR010000000
VERSION     DAOQWR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16807_length_5061_cov_5.032561"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKK36_10585"
     CDS             <1..485
                     /locus_tag="PKK36_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR95038.1"
                     /translation="YRQEYGFNSIYLLPVNLYGPRDNFDPTSSHVIPALIKKIHDAKK
                     AGDSQMVCWGTGSATREFLYVEDCARAIVAASEKYDGSEPVNLGSGMEISIYDLVHLI
                     AKLMDFKGEIVWDRSKPDGQPRRCLDTSRAEKFFGFKASVPFEEGLRKTIEWYTAQVG
                     "
     gene            complement(562..1131)
                     /locus_tag="PKK36_10590"
     CDS             complement(562..1131)
                     /locus_tag="PKK36_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNR95039.1"
                     /translation="MKKLVVFAMAAMMASGVFAATGWYSDYLLVSDNGAADGYYWIGA
                     DPSYGTQFAGSTFEITLGDTFELGADMRYWSDTQDRGGGAFYWTIDNWTTSHEVIWAQ
                     TPLGGNDYQGLADSVTDVASGLGTGSYTLQVYAKSWDTGGGQGDSFLSNGGANYSATL
                     TINPIPEPATFGLLAIGAGLMYLRKRRRA"
     gene            complement(1255..2496)
                     /locus_tag="PKK36_10595"
     CDS             complement(1255..2496)
                     /locus_tag="PKK36_10595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR95040.1"
                     /translation="MKNGAFKIRIAPVIRSLLLCALLPAAVSGQDTAAAASIAMDAGE
                     KTAAEIQAQKHLEQAQHHTSRGNYSAALTEIHDGLSVAPQHPILLQLGAQIYTARGAY
                     ILAEDCWNRLKESDPDNDQYQIGLAGIYVRQGFTKKAEPLLDAALKRDPMSVPARFYL
                     AVVRQHEGKTGEAEKLLRARSSIETGQFASWIDGEYQSVLRLLGRRGFNEFSTIALTG
                     STAENEQGQKRNISEMLKKVKEHIYRSSLAAEEQDWLRAIADLNKARLAGATAPAVYQ
                     DLARYAWMSGKHEQSRGLLESLSARHPEQSSIPSALGILCLEDGQTAAAVRHLKAAVS
                     ADPVNAEARFALACAYAQSEEITKARISLLRLQRLIKKHPGLEISTVPGYAGALRGHA
                     ELLALLKEIFPECNQDTPDTK"
     gene            complement(2543..3850)
                     /gene="creD"
                     /locus_tag="PKK36_10600"
     CDS             complement(2543..3850)
                     /gene="creD"
                     /locus_tag="PKK36_10600"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNR95041.1"
                     /translation="MQTSGSRLKDSVTVKIIIIGFLVLGLLIPAAMVRSLIREREHRR
                     AAVVREISSKWGATQHITGPVLTVPYIEEVRAEKGKPVEVVHHLHILPDMLNISGQVL
                     PEVRYRGIYEAVLYNAQMSLTGEFRRPDLQALDIKPRSVQWQKAFISMGISDLKGVRA
                     AVTMNAGGQDFVMNPGIEAPNLMNAGVGARLSIDPEAETIPFSAALNLNGSTLLSFSP
                     VGRETSVSLSSPWPDPSFTGDFLPANRYIKSDGFTAAWHVLSLNRNYPQTWKDKKYDI
                     TSSSFGVTLVIPADAYQKAERTVKYAALFIVLTFLTLFFSEVMNRIRIHPLQYLLVGL
                     ALIIFYSLLISIAEHLGFAKGYLIASAASIGLITGYARSVLQKNSLALMVGSLLALLY
                     VYLYVLLQLGDYALLFGSIGLFLILAAVMFLTRRINWYTPAGP"
     gene            complement(3900..4703)
                     /locus_tag="PKK36_10605"
     CDS             complement(3900..4703)
                     /locus_tag="PKK36_10605"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNR95042.1"
                     /translation="MIKLPPDYHTHTELCRHAEGRPGDYFRAAAARGLPAVACTDHSP
                     APDGFDPEHRMTTDEFEVYEQWFREEQDRHALPCLFGIEADWYPGCELYMAEWLAAQD
                     FDLVIGSVHYLDYWAFFHSSIGQLWEREDVGQVWRQYFELIRQLTASGMYDIIGHIDL
                     PKKFGHRPPDSEFREIVLPVLDVIAGAGTAIEINTSGFFHPVAEAYPSTQILCWARER
                     NIPITFGSDSHKPERVGNRFEDALALAKEAGYAHSLQFSKRRKTLCPLP"
BASE COUNT         1081 a         1432 c         1460 g         1088 t
ORIGIN      
        1 cctaccggca ggaatacggc ttcaactcca tttatctgct gccggttaac ctttacggcc
       61 ctcgtgacaa tttcgatccg acctcgtctc atgtgattcc cgctctcatc aagaaaatcc
      121 atgatgccaa aaaggcgggc gacagccaga tggtctgctg gggtaccggt tcagccacaa
      181 gagagtttct ttatgtcgaa gactgcgcgc gggctatcgt ggcggccagc gagaaatacg
      241 acgggtctga gcccgtcaat ctcggcagcg gcatggagat cagcatttac gacctcgttc
      301 atttgatcgc gaaactgatg gatttcaagg gagagattgt ttgggaccgg tcgaagcccg
      361 atgggcagcc ccggcgctgt ctggacacct cgcgcgccga gaagttcttt gggttcaaag
      421 cctctgtgcc tttcgaagaa ggcctcagga aaaccatcga gtggtacacg gcgcaggtcg
      481 gctgatcggg aatattaaag ggaaaagaaa agggccggtc tttcgaccgg cccttcaagt
      541 ttgtataatg agatttctga attatgcgcg acggcgcttg cgcaggtaca tcaggcccgc
      601 gccgattgcc agcaaaccga aggtggcagg ctcaggaatc gggttgatcg tgagagttgc
      661 cgaatagttc gcgccgccat tggacagaaa actgtcaccc tgcccgccgc cggtgtccca
      721 gctcttggcg taaacttgca gagtatagga tcctgtgcca agcccggaag caacatcagt
      781 gaccgagtct gcaagcccct gatagtcgtt gccgcccagc ggtgtctgcg cccagatgac
      841 ttcgtgtgag gttgtccagt tgtcaatcgt ccaatagaag gcgccgccgc cccgatcctg
      901 agtgtcactc cagtatctca tgtcggcgcc cagttcaaac gtgtcgccca gtgtgatttc
      961 aaacgttgaa ccggcgaact gtgtgccgta gcttggatcg gcaccaatcc agtaataccc
     1021 atcggccgcg ccattgtcgc tgaccaaaag gtaatccgaa taccaacctg ttgcagcaaa
     1081 cacgccactg gccatcattg cagccattgc aaacaccacc aacttcttca tgacctattc
     1141 tccttattgt ctccgaccaa ttcgatcccc attacactgt ctataatgga ttcttgatgc
     1201 ctctctacat gacagcagat attatgtcaa gcgttttacc ggattatttt aaacttattt
     1261 tgtatccggg gtgtcttgat tacattcggg aaatatctcc ttcagaaggg caagcagctc
     1321 cgcatggccg cgcagagctc ccgcatagcc cggaaccgtg ctgatttcca gacccggatg
     1381 cttttttatg agccgctgaa ggcgaagcag agagatgcgg gcttttgtga tttcctccga
     1441 ttgtgcatag gcgcaggcca gtgcaaaccg cgcctcggcg ttgaccggat cagccgacac
     1501 ggccgctttc aaatgccgga ccgccgcggc ggtctgtccg tcctcaagac ataaaatgcc
     1561 cagcgccgaa ggaatggaag actgctcggg atggcgggcc gaaagggatt ccagcagacc
     1621 gcgcgactgt tcgtgcttcc cgctcatcca tgcatatctg gccaggtcct gatagacggc
     1681 cggggctgtc gcaccggcca gtctggcctt gttcaggtcc gcgatggccc gcagccagtc
     1741 ctgctcttca gccgcaaggc tggagcggta tatgtgttct ttcacctttt tgagcatttc
     1801 ggaaatattg cgcttttgcc cctgctcatt ttcggcggtc gagcccgtca gcgcgatcgt
     1861 cgaaaattcg ttgaaccctc gccggccgag gagccggagc acagattgat attctccatc
     1921 aatccatgaa gcgaactgcc cggtctcgat cgaggagcgc gctcgcagga gtttctccgc
     1981 ctcgccggtt ttcccctcat gctgacggac aacggccaga taaaaccgcg cagggacact
     2041 catcgggtct cttttcaggg cggcatcgag cagcggttct gctttttttg tgaatccctg
     2101 acggacatag attcccgcca ggccgatctg gtattgatcg ttgtcgggat cggattcctt
     2161 cagccggttc cagcagtctt ccgccagaat gtaggctccg cgtgcggtgt aaatctgggc
     2221 gccgagctgg agcaggatgg gatgctgcgg tgcgacgctg aggccgtcat ggatttcagt
     2281 cagcgccgcg gaataatttc cgcgggaggt gtggtgctgc gcctgttcca gatgcttctg
     2341 cgcctgaatc tccgccgccg tcttttcccc ggcatccatg gcgatggagg cggccgcggc
     2401 ggtatcctgc ccggaaacag cggccggcag cagagcgcac aggagcaggc tccggatcac
     2461 tggcgcaatt ctaattttga aggccccgtt tttcatgcgt gcctccagct tcttcgggat
     2521 cggccgggtt gtcaagggcc ggttacggac cggcgggtgt gtaccagttg attcggcggg
     2581 tcaggaacat gaccgctgca aggatcagga acagcccgat actgccgaac agcagcgcat
     2641 agtctcccag ctgaagcagc acatacagat acacataaag aagcgccaga agggagccga
     2701 ccatcagcgc caggctattt ttctgcagca cgctccgcgc atatcccgtg atcagcccga
     2761 tcgaggcggc gcttgcgatc aggtagccct ttgcaaaccc cagatgctcg gcaatagaga
     2821 tcagcaggga atagaaaatg atcagtgcca gcccgaccag cagatactgg agcgggtgga
     2881 tgcggattcg attcattact tcagagaaaa acagggtcag gaacgtcagc acgatgaaca
     2941 gggccgcgta tttgactgtg cgctcggctt tctgataagc gtctgcggga atcaccagcg
     3001 tgaccccgaa ggaggatgag gtaatgtcgt acttcttgtc cttccatgtc tggggatagt
     3061 tccggttcag gctcagcaca tgccatgcgg ccgtgaatcc gtcggatttg atgtagcggt
     3121 tggccggcag aaaatcgccg gtgaaactgg gatccggcca gggagaagac agactgacag
     3181 atgtttcccg gccgaccggg gagaaagaaa gcagggtgct cccgttcagg ttcagcgcgg
     3241 cgctgaaggg aatggtttcg gcctcgggat caatggaaag tcttgcgccg acgcccgcat
     3301 tcatcaggtt gggcgcttcg atgccgggat tcatgacgaa atcctgtccg ccggcattca
     3361 tcgtgaccgc ggcgcggacg cccttcaggt cgctgatccc catggagatg aaggctttct
     3421 gccactgcac ggatcggggt ttgatgtcca gcgcctgcag atcgggccgc cggaattccc
     3481 cggtcaggct catctgcgcg ttatagagca cggcctcata gattccccgg tagcgaactt
     3541 cgggcaggac ttgtccgctg atattgagca tgtcgggcag gatgtgcaga tgatggacca
     3601 cctccaccgg cttgcctttt tcggcccgga cttcctcgat gtacggaacg gtcagaaccg
     3661 gtccggtgat gtgctgcgtt gcgccccatt tggagcttat ttctcgcacc acggcggccc
     3721 ggcgatgctc ccgctcgcgg atcagcgagc gcaccatcgc cgccggaatc aggagtccca
     3781 ggacaagaaa gccgatgatg atgattttta cggtgaccga atctttcagt ctgctgcctg
     3841 aagtctgcat ggcactctcc ttgtcatatg gcggttagac cgcccggcct ccgtgccgtt
     3901 cacggcagcg ggcacaatgt tttcctccgt tttgaaaact gaagcgaatg ggcatatccc
     3961 gcctcttttg ccagcgcgag ggcgtcctca aagcggttcc cgacccgttc aggcttgtgc
     4021 gaatccgagc cgaatgtgat ggggatgttc cgctcgcgcg cccagcacag gatctgtgtc
     4081 gaaggatagg cttcggccac cggatggaaa aagccggagg tgttgatttc gatggcggtt
     4141 ccggcgccgg cgatcacatc gagaaccgga agaacaattt ccctgaactc gctgtcgggc
     4201 gggcggtgtc cgaatttctt gggcaggtca atatggccga tgatgtcgta catcccgctc
     4261 gcggtgagct ggcggatcag ttcaaaatac tgccgccaga cctggccgac atcctcgcgc
     4321 tcccagagct ggccgatgga ggaatggaag aatgcccagt agtccagata atgcacggaa
     4381 ccgatcacca ggtcaaaatc ctgcgcggcg agccactccg ccatgtacag ttcgcatccc
     4441 ggataccagt ccgcctcgat gccgaagaga cagggcaggg cgtgccggtc ctgttcctcc
     4501 cggaaccact gctcataaac ctcaaattca tccgtcgtca tgcggtgctc cgggtcgaac
     4561 ccgtcgggtg cgggcgagtg atccgtgcag gcgacggccg gaagcccgcg cgcggcggcg
     4621 gcgcggaaat agtcgccggg gcgtccctcc gcatgccggc agagctccgt gtgcgtgtga
     4681 taatctggcg gaagctttat catggccgca atgtagcagt cgtgaagggg aaagtcgtta
     4741 ccaaaacttc caatggctgg aactttttac ggaaaaactt ccaatggttg gaacttttcc
     4801 ggcagaaatt tccattcatt ggaacctttg gagggaaccg ctccatcggt tccgtgactc
     4861 tttcagcgga atcataattg acgcgagacg gctctgaaaa ttccggaaaa cggcaaaaaa
     4921 cgctcaaaaa cggctaaaaa ccgcaaaaaa tggcccaaaa tgaccgaaaa catgcatata
     4981 gtactaaaat agtcctatat gaccggatct gcagaaatcg cgatacagag ccatgcccgt
     5041 gaaaaatgcg cgccgtcttt g
//
LOCUS       DAOQWR010000162         5008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_17022_length_5008_cov_5.885524, whole genome shotgun sequence.
ACCESSION   DAOQWR010000162 DAOQWR010000000
VERSION     DAOQWR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17022_length_5008_cov_5.885524"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKK36_10610"
     CDS             complement(<1..820)
                     /locus_tag="PKK36_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR95043.1"
                     /translation="MSAIEARNLGKCYRVRGMGPPTLAGAVKGLFCRRKKEDFWALQG
                     LDFDVQPGKTVGVIGPNGSGKSSLLGLVTGTIFPTTGHVKTSGRIASLLELGAGFHPD
                     LTGRENVYLNAAILGMKREDIRRRMDHIIEFSGLKDFMDQPVKHYSSGMYVRLGFAVA
                     IEMDPDILLIDEVLAVGDLAFQLKCLDRIRDFQRRGKTLLFVSHALQTVEDFCDEVFL
                     IHHGKLVARGDPSDTILQYIRTYMGEGGYLYTQEFGSREVEITDVKLRDGNGNEA"
     gene            complement(817..1614)
                     /locus_tag="PKK36_10615"
     CDS             complement(817..1614)
                     /locus_tag="PKK36_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR95044.1"
                     /translation="MNPLTDLWNRREMLSNLVGRELKSRYKDSALGFLWSILTPLFMA
                     CIYVFFLRLLARGVPMETIIIGVFAWQFTALSVSSGLSAITGNSNLVKKVFFPRIILP
                     TAATLANLVNYVLSLVVQLALVAVLLGLQNKTMNPWVLALPILIVYHTLFNLSLAYFL
                     SAINVYFRDAQHLVGVLLSAWFFATPAMYDLSFVQIFAEKHPWALDVYMLNPMAVIIT
                     GYRAVTLPGVEFPWTGCALAGGLIPLALLGVSYFVFQRMQRNFADML"
     gene            complement(1611..2102)
                     /locus_tag="PKK36_10620"
     CDS             complement(1611..2102)
                     /locus_tag="PKK36_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95045.1"
                     /translation="MAQSRFKYAGLWLAAAMLAAGLLYSSHTVRITPRRIRSIRQKQE
                     VLLQMHDLQKIALAEQAAVRRFEDDASARPCSLKELAGRRADIRQRESVQLAGGWTLY
                     RTEVVFNDTPFDAAGDFIAAAEACRPPWRVTECQFSASPRAPAAGTVTLMLEALEKTG
                     GGQ"
     gene            complement(2089..3498)
                     /locus_tag="PKK36_10625"
     CDS             complement(2089..3498)
                     /locus_tag="PKK36_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95046.1"
                     /translation="MSKLRNRTGYGVDLAGGRAVVVRSASNRRFETVYNGPVGEMGPA
                     VAAAATAVSRGRALASGCQSVAASLTRVLKAPFASVKKAGRVLPSLLDIQLPFPLETC
                     IYRFLDVRRSGDHVRALALAAQDEAVRRRMRQHEEAGMILTSLDHEGLALWTESLEDY
                     PIEPDAVRMVVSLGCENTVLVAGRGLSFESAHHCPVSLDRLRSGETAAADDLVRRMRH
                     VLQAGQFLQAGQKIQWAWTGAGAADEALRSRLESLLELGPEVIRLVHREPETMLARAL
                     SRRAIREGNYAVNLIAGDRANPALEKLRLRRLRGAAVFYFALGLLLTGVNAGWNAWLA
                     TKEAEISRRIGRAAEAIAPGTRIYPGQEFLLVSRAREANAPNVSPFTRVFKPSPAMLL
                     RELLDAAAAADITISKLSLRPDAATVTGASETWAGCEKLSPVFTTYGFMVSEPQREDA
                     GADERVHFSLSAGVSDGAK"
     gene            complement(3503..4477)
                     /gene="gspK"
                     /locus_tag="PKK36_10630"
     CDS             complement(3503..4477)
                     /gene="gspK"
                     /locus_tag="PKK36_10630"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNR95047.1"
                     /translation="MNDLRKPAARKDGMVLVLVLVLLGLITALIVQAQVGALLNLRMR
                     DAELQAARLREVLTTTVLDALQRLADDDLLSVDHLSEPWAQPLEIEYPSGISVWVAVS
                     DAQSGFDLNNLYCEKPPLTAQSPAEVLMNIMTLCGDYAPVERADALRDWIDPDSDGFF
                     ETAYYERREPPFQAADSWLNSWHELAFVQGYSYAYFLSPGQPSQVTTSADLPDCLTVI
                     PGRRASPVPVNINTAPREVLTAVAGLAQEEWARRILVMRQAAPIISLEPLLEAAGPDP
                     DALRPMLDVKSSVFCVKASAFYDSHTADIWALVKRNEDGEVSIIRWVM"
     gene            complement(4474..4770)
                     /locus_tag="PKK36_10635"
     CDS             complement(4474..4770)
                     /locus_tag="PKK36_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95048.1"
                     /translation="MGLFIKGGYRQSKRHRRQTARHQLKKIREAVWVLVWLGLGFALI
                     FGIVALFLKNPSRARWLDFSVIYLAASAVLAGVCGLLKLIELKGYARGRGDDDE"
     gene            complement(4785..>5008)
                     /locus_tag="PKK36_10640"
     CDS             complement(4785..>5008)
                     /locus_tag="PKK36_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95049.1"
                     /translation="FYQINDLYQKISGLYLTTIMRGRPYIGGLRSGADKTWFPILEQR
                     IPADFPLTDGFPIAEMDQMFLTDRERLMK"
BASE COUNT         1052 a         1562 c         1506 g          888 t
ORIGIN      
        1 cggcttcgtt cccgtttccg tcgcgcagtt tcacgtcggt gatttccact tcgcgcgagc
       61 cgaactcctg cgtgtagaga taaccgccct cgcccatgta agtccggatg tactgcagga
      121 tcgtgtcgga cggatctccg cgcgcgacca gtttcccgtg atggatcagg aacacctcat
      181 cgcagaagtc ctcgacggtc tgcagggcat gcgaaacaaa gagaagcgtt ttgccgcgcc
      241 gctggaaatc gcggatgcgg tcgaggcatt tgagctggaa tgcgaggtcg cccacggcca
      301 gcacttcatc aatgagcaga atatcgggat ccatttcgat ggcgaccgcg aagccgaggc
      361 ggacatacat tccgctggag taatgcttca cgggctggtc catgaaatcc ttcagtccgg
      421 agaattcgat gatgtgatcc atccgcctgc ggatgtcctc gcgtttcatg ccgagaatgg
      481 cggcgttcag atagacgttt tcacggccgg tcaggtcggg atggaaaccc gcgcccagtt
      541 ccaggagcga tgcgatccgc cccgaagttt tcacatggcc ggtggtcggg aagatggttc
      601 cggtcacgag gcccagaagc gagcttttcc cggaaccgtt agggccgatc acgccgacgg
      661 tttttccggg ctgcacgtcg aagtcgagcc cctgaagcgc ccagaaatcc tctttcttcc
      721 ggcggcagaa aagccccttc acggcaccgg cgagggttgg aggccccatg ccgcgcacac
      781 ggtagcattt gccgagatta cgggcttcaa ttgcgctcat agcatatccg caaagttgcg
      841 ctgcatcctc tgaaagacga aatacgacac ccccagcagg gccagcggga tcaggccgcc
      901 ggccagcgcg cacccggtcc aggggaactc cacaccgggg agcgttaccg cgcggtaccc
      961 ggtgatgatc acagccatcg ggttgagcat gtacacatcc agcgcccagg gatgcttctc
     1021 ggcgaatatc tgcacaaagg agaggtcata catggccggg gttgcgaaga accacgcgct
     1081 cagcagcacc cccaccagat gctgcgcgtc gcggaaataa acattgatgg ccgacaggaa
     1141 atacgccagc gacagattga acagggtgtg atacacaatc agaatcggca gggccagcac
     1201 ccagggattc atcgttttgt tctgcagtcc cagcagcacg gccaccagcg cgagctgcac
     1261 aaccagcgaa agcacataat tcacaaggtt ggcgagcgtg gcggcggtcg ggaggatgat
     1321 gcgcgggaaa aagaccttct tcacgaggtt ggagttcccc gtgattgccg aaaggccgct
     1381 gctcacggaa agcgccgtga actgccaagc gaaaacgccg atgatgatgg tttccatcgg
     1441 aaccccgcgg gccagcagcc ggaggaaaaa gacatagatg caggccatga aaagcggagt
     1501 gaggatgctc cagagaaaac ccagcgccga gtccttgtag cggctcttca gttcacggcc
     1561 gaccagattg gagagcatct cgcggcggtt ccagagatcg gtcagggggt tcactgtccg
     1621 cctccggttt tttccagcgc ctcaagcatc agcgtcaccg tgcccgcggc gggcgcgcgc
     1681 ggcgaggcgg aaaactggca ttccgtcaca cgccatgggg gacggcatgc ttccgccgcc
     1741 gcaataaagt ctccagccgc atcaaagggg gtgtcgttga acacgacttc cgtgcggtaa
     1801 agggtccagc ctccggccag ctgaacactt tcgcgctgac gaatgtctgc gcggcggccc
     1861 gccagttcct tcagcgaaca ggggcgtgcg gaagcatcgt cctcgaacct tctgacggcg
     1921 gcctgttcgg caagggcaat tttctgcaga tcatgcatct gcagcaggac ctcctgcttc
     1981 tgccggatgc ttctgatccg ccggggcgtg atgcggacgg tgtgcgagga ataaagcaat
     2041 cccgccgcca gcatggcggc cgccagccac aagcccgcat atttgaatct actttgcgcc
     2101 atctgaaact cccgccgaca ggctgaaatg cacgcgctca tcggcgcccg cgtcctcgcg
     2161 ctgcggttca gagaccataa aaccgtatgt ggtgaaaaca ggcgacagtt tttcgcagcc
     2221 ggcccaggtt tccgacgcgc cggtcacggt agccgcatcg ggtcggagcg acaacttgga
     2281 aatggtgatg tctgccgcgg cggcggcatc cagcagttcg cgcaggagca tggccggaga
     2341 cggcttgaag accctggtga aaggactcac gttcggtgcg ttggcctcac gggcgcggct
     2401 gaccagcagg aattcctgcc ccggataaat ccgtgtgccc ggagcaatcg cttccgccgc
     2461 acggccgatc cgccgggaaa tctccgcctc tttcgtggcc agccaggcgt tccatcccgc
     2521 attgactccc gtcaaaagca ggccgagcgc aaaatagaag acggccgcgc cgcgcaggcg
     2581 ccgcaggcgc agcttctcaa gcgcgggatt cgcgcggtct cccgcgatca ggttgacggc
     2641 ataattcccc tcgcggatcg cacgacggct caaagcgcgg gccagcatgg tttccggttc
     2701 gcgatgaaca aggcgtatga cctcgggacc aagttcaagc agtgattcca ggcgggagcg
     2761 aagcgcctca tcggccgcgc cggctccggt ccatgcccac tggattttct ggccggcctg
     2821 cagaaactgt ccggcctgaa gcacatgacg catcctgcgg acgagatcat ccgccgcggc
     2881 cgtctctccg ctccgcaggc ggtccagact caccggacaa tggtgcgccg attcaaagga
     2941 cagaccccgt ccggccacca gcaccgtgtt ttcgcatccg agcgagacca ccatgcggac
     3001 agcatcgggc tcgatgggat aatcttcaag gctctcggtc cagagagcca gtccttcatg
     3061 atcaagggat gtgaggatca ttccggcttc ttcatgctgc cgcatccgcc gacgaacggc
     3121 ttcgtcctgt gcggccagcg cgagggcccg cacatggtcg cccgaccgcc ggacatcgag
     3181 gaaacggtag atgcaggttt ccagcggaaa cggaagctgg atatccagca gcgagggcaa
     3241 caccctcccg gctttcttca ccgacgcaaa gggagctttc agcacgcggg tcaaactcgc
     3301 ggcaaccgac tggcatccgg aagccagcgc gcggcctctt gagacagcgg tcgcggccgc
     3361 cgcaacagcc gggcccattt caccgacggg accgttataa accgtttcaa agcggcggtt
     3421 ggaggcagac cgaaccacga ccgcccggcc gccggccaga tccacaccat aacctgttct
     3481 gttccgcagt ttgctcattc gatcacatca cccagcggat aatggatact tcaccgtctt
     3541 cgtttcgctt gaccagcgcc catatatcgg ccgtatgact atcataaaag gcgctggctt
     3601 tgacacagaa taccgaactt ttcacatcca gcatgggccg aagagcatcc ggatcaggtc
     3661 cggcggcctc cagcaaaggc tccaggctga tgatcggcgc ggcctgccgc atcacaagaa
     3721 tccggcgcgc ccattcctcc tgtgcaagac ctgcgacagc cgtgaggacc tcccgggggg
     3781 cggtgttgat gttcaccggg acgggcgagg cccgacggcc cggaatcact gtcaggcagt
     3841 ccggcaaatc ggccgaggtc gtgacctggg acggctgacc gggcgacagg aaatacgcat
     3901 aggaatagcc ctgcacgaag gcgagttcat gccagctgtt cagccagctg tccgccgcct
     3961 ggaaaggcgg ctcccgccgc tcgtagtagg ctgtttcgaa aaagccgtca gagtccgggt
     4021 caatccagtc gcgcagcgca tcggcacgct ccaccggagc ataatcaccg cagagcgtca
     4081 tgatattcat cagcacttcc gcggggctct gcgccgtcag cggcggtttc tcgcagtaca
     4141 gattgttaag gtcaaacccg ctctgtgcgt cagaaacggc cacccagacg gaaatgccgg
     4201 acggatattc aatctcgagg ggctgggccc acggttcgga aaggtggtcc acggacagca
     4261 ggtcatcgtc ggccaggcgc tgcagggcgt caagaacggt cgtggtgagg acttcgcgca
     4321 ggcgggccgc ctgcagttcg gcatccctca tccgcagatt cagcagcgcc cccacctgcg
     4381 cctgcacaat gagagcggtg atcagaccca gcagaacgag gaccagcaca agcaccatgc
     4441 cgtcttttcg ggctgccggt ttgcgaaggt cattcattcg tcatcatctc ccctgccccg
     4501 ggcatagccc ttgagctcaa tcagcttgag cagtccgcag actcccgcaa gaacggcgga
     4561 ggccgccaaa tagatcaccg agaaatccag ccagcgggcg cgggagggat ttttcaggaa
     4621 cagggccacg atcccgaaga tcagcgcgaa gccgaggcca agccagacca gcacccagac
     4681 ggcttctcgg attttcttga gctgatggcg cgcggtctga cggcggtgcc gtttcgactg
     4741 ccggtaccct cctttgatga acaatcccat ggctgctccg cgttctattt catcagacgt
     4801 tcacgatccg tgagaaacat ctggtccatc tcggcaatag gaaatccgtc cgtcagcggg
     4861 aaatcagccg gaatgcgctg ctccaggatc gggaaccagg tcttgtcggc gccgcttcgc
     4921 agaccgccaa tatagggccg cccgcgcatg atcgtggtca gatacaggcc gctgattttc
     4981 tggtacagat cattgatctg gtaaaacc
//
LOCUS       DAOQWR010000163         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_17583_length_4894_cov_2.839430, whole genome shotgun sequence.
ACCESSION   DAOQWR010000163 DAOQWR010000000
VERSION     DAOQWR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17583_length_4894_cov_2.839430"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKK36_10645"
     CDS             complement(<1..1080)
                     /locus_tag="PKK36_10645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNR95050.1"
                     /translation="MNMEKLLTGKVISVNGPIVKSVGLSGIRMYDIAEVGPDRLIGEV
                     IRLAEEIAIIQVYEDNTGLKPGDEVVSYGRPLSVLLGPGLIASIYDGIQRPLVGISEA
                     CGSYIRKGIKLSPLDAAAKWNFKPLVSSGAAVGEGTVLGEIQESPLVLHRLLVPPGVS
                     GTLTRIAPAGAYTIEEPIYLIDTDAGAYEGKLAAYWPVRRPRPSKKRKKPFIPLVTGQ
                     RVIDTFFPIARGGTAAIPGGFGTGKTMTQHALAKWCNADIIVYIGCGERGNEMTDVLT
                     DFPKLIDERNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRDMGYSVAVMAD
                     STSRWAEALRELASRLGDMPAEEGFP"
     gene            complement(1150..1767)
                     /locus_tag="PKK36_10650"
     CDS             complement(1150..1767)
                     /locus_tag="PKK36_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95051.1"
                     /translation="MDNTSLENSIKRASEEMIAEIREKEARELGRLDEDYAAEIERFR
                     KTMEAETRSRMDQELSRLENRAVLERKKLKLTTVERFIQRIVEDVMRGIRSHPRYRQF
                     LMDAAAGAAAQIPENVEVRLAPDDGVYGNEIDEALRAAGDDRQIRIEADAAIGWGGCL
                     VVDSARGRIFNRTLERIYFRKALQIRRKVLQVLQDRTEGKESVTR"
     gene            complement(1833..2138)
                     /locus_tag="PKK36_10655"
     CDS             complement(1833..2138)
                     /locus_tag="PKK36_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNR95052.1"
                     /translation="MEKIYMIGDMDTVSAFRLAGVEGVVAEPSRAAAALERIVGKKDA
                     GVVLITGALASGLGERITEINLGRPSPVVVEIPGMDDPEGFRGSVMAYVEEALGISL"
     gene            complement(2140..2472)
                     /locus_tag="PKK36_10660"
     CDS             complement(2140..2472)
                     /locus_tag="PKK36_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNR95053.1"
                     /translation="MTRSRQLAFLMGAIFLCALWGLFPAVGWASETNPVKALTDAIGL
                     AYIGAGVAVGFACLASGYAVAKIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLII
                     AIMILNKL"
     gene            complement(2474..4360)
                     /locus_tag="PKK36_10665"
     CDS             complement(2474..4360)
                     /locus_tag="PKK36_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNR95054.1"
                     /translation="MFVKSDIRKITIAFDKEHQRRVYLALGKAGLVHLARFYEKDTVA
                     DGGLQGEESLTREILSATAYSLAALHLEPDERGGEARLVNTAQDEAAASGVKRKVERA
                     LRLQTKIRDQREILARDIEYARALERMGIDAEMLKKPGILQTVFGTVEQTDWLVPSDE
                     RFMIVRTGRFVFGMALPAFAADMMQFLKENGFADQAKDVRTISSAALEKRADILRRRL
                     AALDAYVNRLKEEKGEFLRRMNADYRAYEEVLKAIRLSVISSRAMFLTGWVDAKEKAI
                     LMEIMKEVCGGGFVVSEQKDANAPVRLRNLWLFRPFELLVKTMGMPSSNELDPTPLAA
                     VTYVMMFGLMFGDLGQGIVLMLCGWLLKKFGQKKAREELAQAGGILLACGGSAAVCGL
                     LYGSVFSSEHLLPALWFHPTAQIMRLFFFTIMMGVVFILVGLCLNIINSFVNADYSEA
                     LLEKRGLAILVVYAAAVVFVLQYLKTGQPPALWALGIFMVLPLMIFSLRGLLGVLFLE
                     KAKPHSPSEYIVETIMEVVEIALGLFANTISFIRVGAFALSHAGLSIVTYTLAGMADP
                     SLKSAGAVIIIVCGNIFIIGFEGLICGIQSMRLEYYEFFSKFFKGDGVVFSPFTLPAK
                     ASEV"
     gene            complement(4369..>4894)
                     /locus_tag="PKK36_10670"
     CDS             complement(4369..>4894)
                     /locus_tag="PKK36_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNR95055.1"
                     /translation="ISGYRQLVVHLDDCTARNLYRSAELLAGSAAAEFREMMLKRLAV
                     MTLIWSYRLGVHHRLPQEKVRFYMDKFQALYGGSAWYRVGLEQDALSAYLEQKRKDTG
                     QEPSVTDVEHYLEQTYYAWISRTFHRDFHSIYCVVSYLWLLYYQIRNLFRIIDGRRYG
                     FGADAIIRRMICEA"
BASE COUNT         1032 a         1559 c         1328 g          975 t
ORIGIN      
        1 aggaaatcct tcctcggcgg gcatgtcgcc cagacggctg gccagttcgc gaagggcctc
       61 cgcccagcgc gaggtggaat ccgccatcac ggccacgctg taacccatat cgcggtaata
      121 ttccgcgatg gtcacgccgg tgtagatgct gacttcgcgc gccggcaccg gcatgttgga
      181 cgtgttggcg atcatcaccg tccgctcgat cagcggccgg ccgtttctct catcgatgag
      241 cttcggaaaa tcggtcagga cgtccgtcat ctcattgccg cgctcgccgc agccgatgta
      301 aacgatgatg tccgcgttgc accacttggc cagcgcatgc tgggtcatgg tcttgccggt
      361 tccgaagccg ccggggatgg ccgccgtgcc gccgcgggcg atggggaaaa acgtgtcgat
      421 gacccgctgg ccggtaacaa gcggaatgaa gggcttcttt ctttttttgc tggggcgcgg
      481 cctgcgcacg ggccagtagg cggcaagctt tccttcgtag gcgcccgcgt ccgtatcgat
      541 caaatagatg ggttcttcta tcgtgtaggc cccggccggg gcgatgcggg tcagcgtgcc
      601 ggaaacgccg ggaggcacca gcagccggtg cagcaccagc gggctttcct gaatctcgcc
      661 cagtacggtt ccttcgccga ccgctgcgcc ggatgacaca agcggtttga aattccattt
      721 cgctgccgca tcgaggggag agagcttgat ccccttccga atataactgc cgcaggcctc
      781 ggagattccg accaggggcc gctgaatccc gtcgtagatg ctggcgatca ggcccggccc
      841 cagaagaacg gaaagaggcc ggccgtagga aaccacctca tcccccggct tcaggcccgt
      901 attgtcttca tacacctgaa tgatggcgat ttcctccgcc aggcggatca cctcgccgat
      961 gaggcggtcc ggtccaacct cggcgatgtc gtacatccgg atccccgaaa gccccacgga
     1021 cttgacaatc gggccgttga ccgaaatcac ttttcccgtc agcagttttt ccatgttcac
     1081 tcctgaaaag ccgccccgcg ccggttcagg gcgccggacc gcaggcaacg atggaaagcg
     1141 gccggaccgt tatcgggtca cggattcctt cccctccgtt ctgtcctgca gaacctgcag
     1201 gacctttcgc cgaatctgca aagccttccg gaaataaatt ctttccagcg tgcgattgaa
     1261 aatgcgtccc cgggcagaat ccaccaccaa gcagccgccc cacccgatcg ctgcatcggc
     1321 ctcaatgcga atctgccgat cgtcccccgc ggcgcggaga gcctcgtcaa tctcgtttcc
     1381 gtagactccg tcgtccggtg caagccgcac ttccacattt tcgggaattt gcgcggcggc
     1441 gccggctgcc gcatccatga gaaattgccg gtagcgcgga tggctccgga tccctctcat
     1501 cacatcctcg acaatccgtt gaatgaatcg ctccaccgtg gtcagcttga gcttttttct
     1561 ctccagaacg gctctgtttt ccagccggga aagctcctga tccatgcgcg aacgcgtttc
     1621 ggcttccatc gttttgcgga aacgctcaat ctccgccgca taatcctcgt ccagccgccc
     1681 aagctcgcgg gcttccttct cgcggatttc ggcaatcatt tcctccgacg cccgtttgat
     1741 ggaattttcc agggaggtgt tgtccatatt caatccttct ttgtgtcggg aacggtttca
     1801 aaccgttccc gacagtgatc cggcgatgat gctcacaacg atatccccag ggcctcctcc
     1861 acgtacgcca tcaccgatcc gcggaagcct tccgggtcgt ccatgccggg aatctcgacc
     1921 accaccgggg acggtctgcc caggttgatt tccgtaatgc gctccccgag tccggaagcc
     1981 agcgcgccgg tgatgagcac cactcccgcg tcttttttcc cgacgattct ttcgagcgcg
     2041 gcggcggccc gtgaaggctc cgccacgacg ccctccacgc cggccaggcg aaaagcgctg
     2101 acggtatcca tgtctccgat catgtaaatt ttttccatat tacagtttat tcaggatcat
     2161 gatggcgata atcaggccgt aaatggcaat gccttccgcc agccccagaa ataccagggt
     2221 gcgccccatc agttccggtt tttcgctcac cgccccgatg gccgcggagc cgatcttggc
     2281 caccgcatag ccgctggcca gacaggcaaa cccgacggcc acccccgcgc cgatgtaagc
     2341 cagcccgatc gcgtccgtaa gcgccttgac cggatttgtc tcggacgccc atcccacggc
     2401 cggaaagaga ccccacaggg cgcacaggaa aatcgccccc atcagaaacg ccaactgcct
     2461 gctcctggtc atttcacacc tccgatgctt ttgccggtaa cgtaaacggg gaaaagacca
     2521 ccccgtctcc cttgaaaaac ttgctgaaaa actcgtaata ttcgagacgc atggactgaa
     2581 tgccgcaaat cagcccctcg aagccgatga tgaaaatatt gccgcacacg atgatgatga
     2641 cggcgcccgc ggatttcagg gacgggtcgg ccatccccgc aagcgtatag gtgacaatgc
     2701 tcaagcccgc gtgcgaaagc gcaaaggcgc ccacgcggat aaaggaaatg gtattggcaa
     2761 aaagacccag cgcaatctcc accacctcca tgatggtttc aacgatgtat tccgatgggc
     2821 tgtgcggttt ggctttttcc aaaaacaaca cacccagcag gccgcgcagc gaaaagatca
     2881 tcaggggaag gaccataaaa atccccagcg cccataaggc gggaggctga ccggtcttca
     2941 ggtactgcag gacgaatacg acggcagccg cgtaaacgac gaggatggcc agcccccgct
     3001 tctccaggag ggcttcggaa taatccgcat tgacaaaact gttgatgatg ttgaggcaaa
     3061 ggccgaccag gataaacacc acgcccatca tgatggtgaa gaaaaacagg cgcatgatct
     3121 gagccgtggg gtgaaaccac agagcgggca ggaggtgctc gctggaaaaa acactgccgt
     3181 acagcagacc gcagacggcc gccgagccgc cgcaggccag aagaatacct cccgcctggg
     3241 ccagttcttc gcgggctttc ttctgaccga attttttcag cagccagccg cacagcatca
     3301 gcacgatgcc ctggccgaga tcgccgaaca tcagtccgaa catcatgaca taggtaacgg
     3361 ccgcaagcgg cgtgggatcc agctcgtttg aagagggcat ccccatggtt ttgacgagca
     3421 gctcaaaggg ccggaacagc cacaggtttc tcaatcggac aggggcgttg gcgtccttct
     3481 gctccgacac gacaaacccg ccgccgcaaa cctccttcat gatttccatg aggattgcct
     3541 tttcctttgc gtccacccac cccgtgagaa acatggcccg cgatgaaatc acggacaggc
     3601 gtatggcttt gaggacttcc tcataggccc ggtaatcggc gttcatccgc cggagaaact
     3661 ctcctttttc ctctttcagg cggttgacgt aggcatccag cgccgcaaga cgccgccgca
     3721 aaatatccgc gcgcttttcc agcgcagcgg aagaaatagt ccggacatcc ttcgcttggt
     3781 cggcaaatcc gttttccttc agaaactgca tcatgtcggc ggcaaaagcc ggcagggcca
     3841 tcccgaacac aaagcgcccc gtcctgacga tcataaaacg ctcgtcggac ggaaccagcc
     3901 agtcggtctg ttccaccgtc ccgaagaccg tttgaagaat gcccggtttt ttcagcattt
     3961 ccgcgtcgat gcccatgcgc tccagcgcgc gggcgtattc aatgtcccgc gccagaattt
     4021 cccgctgatc ccggattttt gtctgaagcc gcagcgcgcg ctccaccttt ctttttacgc
     4081 cggaagccgc cgcttcatcc tgcgccgtgt tcaccagcct ggcctccccg ccgcgctcat
     4141 ccggctccag atgcagggcg gcaagggaat aagcggttgc cgaaaggatc tctcgggtca
     4201 gcgactcctc gccctgcagt cctccgtccg ccacggtatc tttctcgtaa aaacgggcaa
     4261 gatgaacgag ccccgccttc cccagcgcaa gataaaccct gcgctgatgt tctttgtcga
     4321 aagcaatcgt tattttccgg atatccgatt ttacaaacat acccgacctc acgcttcaca
     4381 gatcattctg cggatgatcg catcggcgcc aaacccgtag cgccggccgt cgatgattcg
     4441 aaaaagattt cgaatctggt aatacagaag ccacagatag gagaccacgc agtagatgga
     4501 gtgaaaatcc cggtggaacg tgcgggaaat ccatgcgtag tatgtctgtt caagataatg
     4561 ttcgacatcc gtgacggagg gttcctgccc cgtatctttc cttttctgct cgagataggc
     4621 cgaaagcgcg tcctgttcca gaccgacccg gtaccaggcg ctgcctccgt aaagcgcctg
     4681 aaacttatcc atgtagaacc ggactttttc ctgcgggagg cggtgatgca cgccgagccg
     4741 gtacgaccag atcagcgtca tcacggccag ccttttgagc atcatttcgc gaaattccgc
     4801 ggcggcggaa ccggcaagca attcggcgga ccggtaaagg ttccgggcgg tgcagtcatc
     4861 caggtgaacc accagctgcc ggtagccgga aatg
//
LOCUS       DAOQWR010000164         4848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_17771_length_4848_cov_5.097016, whole genome shotgun sequence.
ACCESSION   DAOQWR010000164 DAOQWR010000000
VERSION     DAOQWR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17771_length_4848_cov_5.097016"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKK36_10675"
     CDS             <1..466
                     /locus_tag="PKK36_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNR95056.1"
                     /translation="LWLFNAWMFLQGIETLPLRMQRHCENALAAAKHLKSHKRVAWVR
                     YPGLLGDEAHANAEKYLPNGAGGMVVFGIRGGSRAGAAFINSLQLISHLANVGDARSL
                     AIHPASTTHSQLTGEQQAQGGITPDMVRLSIGIEHIDDILEDLDRALAASAS"
     gene            521..1669
                     /locus_tag="PKK36_10680"
     CDS             521..1669
                     /locus_tag="PKK36_10680"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNR95057.1"
                     /translation="MKKVETQFYTFGKTRKDRLTLKSGVLFGPVTVAYETYGRLSPAR
                     DNAILLFHALSGSQHAAGINKSVPGVGRLWQEECHVGWWNDYIGPGRALDTSQFFIIC
                     ANYFGGCYGSTGPSSINPRTGRPYGARFPRIEANDMVDVQVRLLRHLGIDRLHAAIGA
                     SMGGMLAVNLAVRYPHLVANVVPIAAGLEVSTLQRIHNFEQVYAIESDPHYNRGDYYD
                     GPGPVDGLALARMISHKTFVSLAALTSRARTEVVDRNARFKTYPLHYPVESYLLHQCQ
                     KFVKRFDANTYMLIIDVWQCYDLLKDTGAEDYKTLFAPCRHQKYLVFTIDSDVCFYPD
                     QQEFMARTLTRCRVDCQRVTVHSDKGHDAFLLEPKLFTPHIDYALRRG"
     gene            1673..2554
                     /gene="purU"
                     /locus_tag="PKK36_10685"
     CDS             1673..2554
                     /gene="purU"
                     /locus_tag="PKK36_10685"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNR95058.1"
                     /translation="MSGPGQKEQSRETAILLISCPDAIGIDAAVTNFLSSHRGNIVDF
                     DQHVDKEQNIFFLRVEWEMEGFSLSKEKTEAEIAVLAKPFNMSWSLRYSSAVPRMAVF
                     VSKQPHCLYDLLARVQSGEWRVEIPLIISNHQDMEPLARRAGIPFFHIPVSRETREAA
                     ETRQIALLKENRVDFIVLARYMQILTPQIIAEFPGRIINIHHSFLPAFPGAKPYHSAH
                     ARGVKIIGATSHYVTEDLDAGPIIEQDVIRVSHKDSVDDLVRRGRDLEKIVLSRAVWR
                     HLQSKILVYSNKTVVFG"
     gene            2561..3004
                     /locus_tag="PKK36_10690"
     CDS             2561..3004
                     /locus_tag="PKK36_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR95059.1"
                     /translation="MRVSTKGRYGLRVLLDIALHQQDRPVALRDIAERQDISQKYIWQ
                     VVSPLKAAGLLNTVRGAQGGCLLARDPDQITMLEVVEILEGPVALLDCLKDEALCRRS
                     SACSTQMAWRKVTKAMRKAMGEVTLKKLMRWQNECEENSSSSYVI"
     gene            3111..4403
                     /locus_tag="PKK36_10695"
     CDS             3111..4403
                     /locus_tag="PKK36_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNR95060.1"
                     /translation="METKGINTLALHAGQEPDPTTGSRAVPIYQTSSYVFKSSEHAAN
                     LFALKEFGNIYTRLMNPTTDVFEKRVAALEGGTGALGVASGQSAITYALLAITRLGDE
                     VVAANNLYGGTYQLFNHTLPRLGRKVVFVDSRDVNAFRSAVTPKTRAIYVETIGNPKL
                     DVPDFEAIAAIAHKNGIPLIADNTVGVGLVKPFDHGADIIVSSATKYIGGHGTSVGGV
                     IVDSGKFKWNNGKFPEFTEPDPSYHGIVYWDALGDVPGMGNVAFILKIRVTLLRDIGA
                     ALSPFNAHEFLLGLETLPIRQKRHSENALEIAGWLQKHPLVSWVTYPGLRNDPGYANA
                     SKYLKHGFGGLVGFGIKGGLESGRKFIDSVKLLSHLANIGDAKSLVIHPASTTHQQLT
                     PEEQQSTGVTADYIRLSIGLEDIEDIKADIDQALKKAV"
     gene            4420..4686
                     /locus_tag="PKK36_10700"
     CDS             4420..4686
                     /locus_tag="PKK36_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNR95061.1"
                     /translation="MRSENVTVRFEHGLHMRVAAQIAKIAQRFNAAVHIRKEGRPQAN
                     AGSILEIMSLGATAGEHLSVSADGPEKDEAVRAVTGLFDQGGGI"
     gene            4708..>4848
                     /locus_tag="PKK36_10705"
     CDS             4708..>4848
                     /locus_tag="PKK36_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNR95062.1"
                     /translation="MSRIYANNAESIGNTPLVRLNRITQGSRAVVLGKVEGRNPAFSV
                     KCR"
BASE COUNT         1052 a         1388 c         1452 g          956 t
ORIGIN      
        1 cttgtggctt ttcaatgcgt ggatgttcct gcagggcatc gagacgctgc ccctccggat
       61 gcagcggcac tgcgagaacg cgctggccgc tgccaagcac ctgaaaagcc acaagcgcgt
      121 ggcgtgggtg cgctatccgg ggctgctggg cgacgaggct catgccaatg cagagaaata
      181 cctcccgaac ggcgcgggcg gcatggtggt gtttggcatc aggggcggca gcagggccgg
      241 agctgcgttt atcaacagcc tgcagctcat atcgcatctg gccaacgtgg gcgatgccag
      301 aagcctcgcg atccacccgg ccagcacgac gcacagccag ctcaccgggg agcagcaggc
      361 gcagggcggc atcactccgg acatggtgcg cctgtccatc ggcattgagc atattgacga
      421 catcctggag gaccttgacc gggctctggc cgcatccgcc tcttgaccgc ggaatcaaaa
      481 ggtgcgagcc tgtgtgccgt tttgatcaga agcgggatgc atgaaaaaag ttgaaacaca
      541 gttttacacc ttcgggaaaa cccggaagga ccggctcacg ctgaaaagcg gggtcttgtt
      601 cggtcctgtg actgtggcct atgaaacgta tggccggctg tccccggccc gcgacaacgc
      661 gatcctgctt tttcatgcgc tgtccggcag ccagcatgcc gcgggcatca acaaatcggt
      721 gcccggggtc ggccggctct ggcaggagga gtgccatgtc ggctggtgga acgattatat
      781 cgggccgggc cgggcgctgg acaccagcca gtttttcatc atctgcgcca actatttcgg
      841 cggatgctac gggagcacgg gcccgtcatc catcaacccc cgcacgggcc gtccctacgg
      901 ggcgcgattc ccccgcattg aagccaatga catggtggat gtgcaggtcc gcctgctccg
      961 gcatcttggc attgatcggc tgcatgcggc catcggggcc tccatgggcg gcatgctggc
     1021 cgtgaatctt gccgtgcgct atcctcatct ggtggccaat gttgttccaa tcgccgcggg
     1081 tctggaagtt tccaccctgc agcgcattca caatttcgaa caggtttatg ccatcgaaag
     1141 cgatccgcac tacaaccgcg gcgactacta tgacgggccc ggtcccgtgg acggccttgc
     1201 gctggctcgc atgatttcgc ataagacttt tgtttctctc gcggccctga ccagccgcgc
     1261 gcggacggaa gtggtggacc ggaatgcaag attcaagacc tacccgctgc actatccggt
     1321 ggagtcctac ctgctgcatc agtgccagaa gttcgtgaag cggtttgatg ccaacaccta
     1381 catgctgatc attgatgtct ggcagtgcta cgacctgctg aaggacaccg gcgcggaaga
     1441 ttataaaacc ctgtttgcgc cgtgccgcca tcagaagtat ctggtgttca cgattgattc
     1501 ggacgtctgc ttctacccgg atcagcagga gttcatggcg cggacgctga cgcgctgcag
     1561 ggtggactgc cagcgcgtga ccgtgcattc ggacaagggg cacgatgcgt ttctgctcga
     1621 acccaagctt ttcactcccc acattgatta tgccctgcgg cgaggataaa ccatgagcgg
     1681 acccggacag aaggaacaaa gccgcgagac agccattctg ctgatttcgt gcccggatgc
     1741 catcggcatt gatgcggccg tgacgaactt tctgagcagc catcgcggca acatcgtgga
     1801 tttcgaccag cacgtggaca aggagcagaa catcttcttc cttcgcgtgg aatgggaaat
     1861 ggaaggtttt tcgctttcca aagagaagac agaggcagaa atcgccgtcc tcgccaaacc
     1921 cttcaacatg tcgtggtcac tgcgctattc cagcgcggtc ccgcgcatgg cagtttttgt
     1981 ctcaaagcag ccccactgcc tgtatgatct tctggcgcgt gtgcagtcgg gggagtggag
     2041 ggtcgaaatc cccctgatca tcagcaatca tcaggacatg gagccgctgg cgcgccgcgc
     2101 cgggatacct ttcttccaca tcccggtttc gcgcgaaacc cgggaggcgg cggaaacgcg
     2161 ccagatcgcg ctgctgaagg aaaaccgggt ggacttcatt gtccttgcgc gctacatgca
     2221 gattctgacg ccgcagatca ttgccgaatt ccccggccgc atcatcaaca ttcaccattc
     2281 attcctgccg gcgtttcccg gcgcaaagcc ctatcattcc gcccatgcac gcggggtgaa
     2341 gatcatcggc gcgaccagtc actatgtcac ggaagacctg gatgccggtc cgatcatcga
     2401 gcaggatgtg atccgcgtga gtcacaagga cagcgtggac gatctggtgc gccggggccg
     2461 tgatctggaa aagattgttc tttcccgcgc ggtctggcgg catctgcaga gcaaaattct
     2521 ggtctacagc aacaaaaccg ttgtgtttgg ctgaggtgcg atgagagttt caacaaaagg
     2581 ccggtacgga ttgcgggttc ttctggacat cgcccttcat cagcaggatc gtcccgtcgc
     2641 gcttcgggac attgccgagc ggcaggacat ctcgcagaaa tacatctggc aggttgtgag
     2701 tccgctgaag gcggccggac tgctgaacac ggtgcgcggc gctcagggcg gctgcctgct
     2761 ggcccgggat ccggatcaga tcaccatgct cgaagtggtg gaaatcctcg aaggtcccgt
     2821 tgccctgctg gactgcctga aagatgaagc cctgtgccgc cgcagttcgg cctgttccac
     2881 acagatggcc tggcggaaag tcacaaaggc gatgcggaag gcgatggggg aagtaaccct
     2941 gaaaaaactg atgcgctggc agaacgagtg cgaggaaaac tcctccagca gctacgtcat
     3001 ctgacctgca aatatttcat aaaaatactt gaaaaacgaa gttctgacct tatagtctag
     3061 aaaattgatg gagatagtat atattaaatt gaaccagcaa agggaaagca atggaaacta
     3121 aaggcattaa tacactggca cttcatgcgg gacaggagcc cgatccgacc accggatcgc
     3181 gagccgtccc gatttatcag acttcatcct atgtgttcaa gagctccgaa cacgccgcga
     3241 acctgtttgc gctcaaggag tttggcaaca tctatacccg cctcatgaac cccaccacgg
     3301 acgttttcga gaagcgtgtg gccgcgctgg agggcggcac gggagctctg ggcgtggcct
     3361 ccggccagtc cgccatcact tatgccctgc tggccattac acgcctgggg gatgaagtcg
     3421 tggcggccaa caatctctac ggcggcacct accagctttt caaccacaca ctcccccggc
     3481 tgggccgcaa ggtcgttttc gtggattcgc gcgacgtgaa cgctttccgc agcgcggtta
     3541 cccccaaaac gcgggccatt tatgttgaaa ccataggcaa tcccaaactg gatgtgccgg
     3601 acttcgaggc cattgccgcg atcgcccata aaaacgggat cccactgatc gccgacaaca
     3661 ccgtgggtgt cggactggtg aagccgttcg atcacggggc agacatcatt gtgagctccg
     3721 cgacaaaata catcggcggg cacggcacat ctgtgggagg tgtgattgtg gattccggaa
     3781 agttcaaatg gaacaacggc aaattccccg agttcaccga gcccgatccc agctaccacg
     3841 ggatcgtgta ctgggatgcc ctcggagatg tgcccggcat gggcaatgtg gcgttcattc
     3901 tgaaaatccg agttactctg ctgcgcgata tcggcgcggc cctcagcccg ttcaacgcgc
     3961 acgagtttct gctcggtctg gaaacccttc cgatccgtca gaagcggcac tcggagaatg
     4021 cgctggagat cgcaggctgg ctgcagaagc atccgcttgt gagctgggtc acctatccgg
     4081 gactcaggaa tgatcccggc tatgccaacg cgtcgaaata tctgaagcac ggcttcggcg
     4141 gactggtggg cttcggcatc aagggcggtt tggaaagcgg acgaaaattc attgattccg
     4201 taaaactgct ttcgcatctg gccaacattg gcgatgcgaa gagcctggtg attcatccgg
     4261 cgtccacaac ccaccagcag ctcacgccgg aggagcagca gtccaccggt gtcacggcgg
     4321 attatatccg gctttccatc ggactggagg atatcgagga catcaaggcg gacatagacc
     4381 aggccctgaa aaaggcggtt tagaaaaccg gagcacggca tgagaagcga gaatgtaaca
     4441 gttcgatttg agcacggcct gcatatgcgg gtggcggcgc agatcgcaaa gattgcgcag
     4501 cgcttcaatg cggccgtgca tattcggaag gaaggccggc ctcaggcgaa tgccggctcg
     4561 attctggaaa tcatgagcct gggcgcgacg gccggggagc atctctctgt gtctgccgac
     4621 ggaccggaga aagacgaggc ggtgagggct gtcaccggtc tctttgatca gggcggagga
     4681 atttaacata cagagaagga gaacatcatg agccgtatat atgcaaacaa cgcagagtcc
     4741 atcggaaaca cgccgctggt ccgactgaac cggatcacgc agggcagccg ggccgttgtg
     4801 ctgggcaaag tcgagggacg caacccggcc ttttcggtca agtgccgc
//
LOCUS       DAOQWR010000165         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_17871_length_4829_cov_5.172811, whole genome shotgun sequence.
ACCESSION   DAOQWR010000165 DAOQWR010000000
VERSION     DAOQWR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_4829_cov_5.172811"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PKK36_10710"
     CDS             <1..1340
                     /locus_tag="PKK36_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNR95063.1"
                     /translation="TRPDYLKLRRHPLFAESKSNFMLFAPVPYGPYTVIDPADVLAEE
                     KTRLVIQPRRRAGSVNVDSSRVIVRSRAAGYFMGTSDQQVNGMNLTGVWPLDRNLEEA
                     MEMRFSNREAPAVTQTIKGPRGTAEISLSPYTPRDPPPGDVRLILREPVSDSRAHVIM
                     LNGDFSIADSKAWNKLDPQLQEQVLAVIPGRTGQAVSSSGDLYDVTVKEPQVRWPHRP
                     VRGHLLGIDTAGRDVFARILYGLRISMTFGFLLVLCSMCIGIFMGAVQGYHGGRLDIT
                     IQRFIEIWSALPFLYIMILMGSIYGRSFLLLLFCYGIFNWIGISYYMRGEFLRLRRQA
                     FVDAARCMGIPSLKIMFRHIFPNALIPVVTFFPFSLVGAIGSLTALDYLGFGLPPPTP
                     SWGELLQQAQQFRWAWWLILYPSLALFIVMLLGVFVGEGVRDAYDPKPFSRME"
     gene            1343..2329
                     /locus_tag="PKK36_10715"
     CDS             1343..2329
                     /locus_tag="PKK36_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR95064.1"
                     /translation="MEEKANILDIRDLSVSFRTDEGLVQAADRVSFSIRRGETLGLVG
                     ESGCGKSVTAMSILRLIPSPPGRIDSGEILFRGQDLLKLPIEELRRVRGKSISMIFQE
                     PMTALSPLQRIGRQMVEALQLHSRISRREAWQIGTEWLRRVGMPNPEEQMWAWPHNLS
                     GGMRQRVLIAMALMLEPALIIADEPTTALDVTIQAQIFDLMRRMKKENESLLLITHDM
                     AVIWEMCDRVAVMYASEIVETGTVEQVFRNPLHPYTRALMAAIPSASSKGRELYHIKG
                     QVPPGINYPAGCRFRERCPIAIERCGMEHPMLDPYGDQCARCFRAGEKGVAL"
     gene            2326..3294
                     /locus_tag="PKK36_10720"
     CDS             2326..3294
                     /locus_tag="PKK36_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR95065.1"
                     /translation="MNPLLQIRDLQTWFPIHRGVISRTVGNVRAVDGVSLDIYPNETL
                     GLVGESGCGKSTLARTVALLEKPRAGTMHFSGQVLSQAQPGIRRKIQMIFQDPYSSLN
                     PRMTVLEILTEGLVQHRRIKSSGRVTAAREILADVGMDPDVLYRYPHEFSGGQRQRIS
                     IGRALSLKPELLICDEAVSALDVSVQAQVINLLLSIRRKHGLSYLFISHDLGVVRHMA
                     DRVAVMYLGQIVEEGPADKIMDAPAHPYTQALISAIPRIGAEKGKRIVLQGDVPSPAA
                     PPPGCRFHPRCPYAQDICRREQPELKGYEGGDSDRKAACFFAGRVG"
     gene            complement(3291..4301)
                     /locus_tag="PKK36_10725"
     CDS             complement(3291..4301)
                     /locus_tag="PKK36_10725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNR95066.1"
                     /translation="MKVGEFVGRFMDQRRQKRSVKEFFESGSMLCEQCGHVLSLAGLK
                     PFSFTECPKCAFKNFVPMRMGDYMLFQPVGAGGNASVYKAWRKDDTEHLFAAKILQPE
                     RARDRETLDNFLLEVRIHEDVNNHPNIATFYGHGIEEDYHYFIMDFIEGEGLKHILSH
                     KGKLDEVRALDILAQVISAMKHIYKAGYLYRDLSAGNVIVQPNGHAVLIDFGLTLPRE
                     EASRKGTETYVDGTAEFIPPERIYRRGEDAPSVIYSLGMLLYYMLTAEPLIKATTLVG
                     KAKRHVSNLRLAITPDMLPGNSEDTAGLLGIMIEPDPDDRFQTFEDLEAAVNGIRARQ
                     KV"
     gene            complement(4298..>4829)
                     /locus_tag="PKK36_10730"
     CDS             complement(4298..>4829)
                     /locus_tag="PKK36_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95067.1"
                     /translation="TVTAVYREALDALKEGSWTAARADGWMARLETVFNRGFWEGGYY
                     CGERLGEWSGMGNSQASVKRVQIGLVEHFYPAPRVMQFTMMQRELKPGAVVLVEGPTT
                     GAIRQIVQHLRVDGRPAARAVKGDVVTLEIPAKARLKDKVFLHVPAAADNEKEFLNGL
                     CVMGNITETENDQRNL"
BASE COUNT          905 a         1472 c         1456 g          996 t
ORIGIN      
        1 ggacccgccc cgattatctg aagctgcgca ggcatcccct cttcgcggag agcaaaagta
       61 atttcatgct cttcgctccc gttccctacg ggccgtacac agtcattgat cccgcggatg
      121 tcctcgcgga agagaaaaca cgcctggtga ttcagccccg ccgccgcgcc ggcagtgtca
      181 acgtggactc gagccgtgtc atcgtgcgca gccgcgccgc gggctatttc atgggaactt
      241 cggaccaaca ggtcaacggc atgaacctga ccggcgtctg gccgctggac cggaatctgg
      301 aagaggccat ggaaatgcgg ttcagcaacc gggaagcgcc cgccgtcacg cagacgatca
      361 aaggcccacg cggcaccgcg gaaatttcgc tgtcgccgta tacaccccgt gatccgccgc
      421 ccggggatgt ccgcctgatc cttcgcgaac cggtttctga cagccgggcc catgtgatca
      481 tgctgaacgg cgatttcagc atcgcggact cgaaagcatg gaacaaactc gacccgcagc
      541 ttcaggaaca ggtgctggcg gtcatccccg gccggacggg ccaggccgtt tcgtcgagcg
      601 gcgacctgta tgatgtgacc gtgaaagagc cccaggttcg ctggccgcac cggccggtgc
      661 ggggacacct gctcggcata gacaccgccg ggcgcgatgt gtttgcaagg attctctacg
      721 gcctgcgcat ttccatgacc ttcggctttc tgctggtgct ctgttccatg tgcattggga
      781 tcttcatggg cgcggttcag ggctatcacg ggggcagact ggacatcacc atccagcgct
      841 tcattgaaat ctggagcgca ctgcccttcc tttacatcat gattctgatg gggtccatct
      901 acgggcgcag ttttctgctc cttctgttct gctacggcat tttcaactgg atcggcattt
      961 catactacat gcggggggaa tttctgcgcc ttcggcgtca ggcattcgtg gatgcggcgc
     1021 gctgcatggg gattccttca ctcaaaataa tgtttcggca cattttcccg aatgcactga
     1081 tccccgtggt cacttttttc ccgttttcac tggtgggcgc gatcgggtcg ctgaccgcgc
     1141 tggactatct gggcttcggc ctgccgccgc cgaccccgag ctggggcgag ctgctgcagc
     1201 aggcacagca gttccgctgg gcatggtggc tgattcttta tccgtcgctg gcgctgttca
     1261 tcgtcatgct tctgggggtt ttcgtgggag agggcgtgcg cgatgcgtat gatcccaagc
     1321 ccttctcccg aatggaatag acatggaaga aaaagcaaac attctggata tccgggattt
     1381 gtcggtttcg ttccgcacgg acgagggtct tgtgcaggcg gccgaccgtg tttctttttc
     1441 cattcgcagg ggcgaaacgc tggggctggt tggagaatcc ggctgcggca agtcggtgac
     1501 ggccatgagc attctgcgcc tgattccgtc ccctccgggc cgcattgatt ccggagaaat
     1561 cctgttccgg ggacaggatt tgctgaagct gcccattgaa gagctgcgcc gtgtgcgcgg
     1621 caaatccatc agcatgatct tccaggagcc gatgaccgcg ctctcgccgc tgcagcggat
     1681 cgggcggcag atggtggagg cgctccagct ccattcccgc atcagccgca gggaggcatg
     1741 gcagatcgga accgaatggc tccgccgggt gggcatgccg aatcccgagg agcagatgtg
     1801 ggcatggccg cacaatcttt ccggcggcat gcgccagcgg gtgctgatcg ccatggcgct
     1861 gatgctggaa cctgcgctga tcattgcgga cgagcccacc acggcgctgg acgtgacgat
     1921 tcaggcgcag attttcgacc tgatgcggcg gatgaaaaag gagaacgaat cgctgctgct
     1981 gatcacgcac gacatggccg tcatctggga gatgtgcgac cgggtcgccg tgatgtacgc
     2041 atcggagatt gttgaaacag gcaccgtgga gcaggtcttc agaaaccctc ttcatcccta
     2101 cacgcgcgcg ctcatggccg ccatcccgtc ggcctcctca aagggccggg aactgtacca
     2161 catcaagggc caggtgccgc cgggcataaa ctatccagcg ggatgccgct tccgcgagcg
     2221 atgcccgatc gctattgaac ggtgcggcat ggaacacccg atgctggatc cgtacggcga
     2281 ccagtgcgcg cggtgctttc gcgcggggga aaagggggtg gcgctatgaa tccgctcctg
     2341 caaatacgcg atctgcaaac gtggtttccc attcaccggg gcgtgatctc gcgcaccgtg
     2401 ggcaatgttc gcgcggtgga cggcgtttcg ctggacattt atccgaatga gactctgggc
     2461 ctggtgggcg aatcgggctg cgggaaatcc acactggctc gaaccgttgc cctgctggaa
     2521 aaaccgcgtg ccggcacgat gcatttcagc ggacaggtcc tgtcgcaggc gcagcccggt
     2581 atccggagaa agattcagat gatcttccag gatccctatt cctcgctgaa tccccggatg
     2641 accgtgctgg aaatcctgac ggaaggactg gttcagcatc gccgcatcaa atcgtccgga
     2701 cgcgtaacgg ccgcccgcga aatccttgcg gatgtgggca tggatccgga tgtcctgtac
     2761 cgctatcccc acgagttctc cggcgggcag cggcagcgga tcagcatcgg ccgggcgctc
     2821 tccctgaaac ccgaactgct aatctgcgac gaggcggtca gcgcgctgga tgtttccgtg
     2881 caggcgcagg tgatcaacct gctgctctcc atccgcagga agcacggcct ttcctatctg
     2941 ttcatatccc acgacctggg ggtggtgcgg cacatggccg accgggtggc ggtgatgtat
     3001 ctcgggcaga ttgtggagga agggccagcg gacaaaatca tggacgcccc ggcacatccc
     3061 tatacacagg cgctgatctc cgcaattccg cggatcggcg cggagaaggg aaaacgaatt
     3121 gtgctgcagg gcgatgtccc ctcgcctgcc gcgccgcccc cgggctgccg tttccatccc
     3181 cgctgtcctt atgcgcagga catctgccgg agggaacagc ctgaactcaa ggggtatgag
     3241 ggcggagatt ccgaccggaa agcggcctgc ttcttcgcgg ggcgggtggg ttaaaccttc
     3301 tgccgcgcgc ggatgccgtt gacggccgct tcgagatctt caaacgtctg gaaccggtca
     3361 tcggggtccg gctcaatcat gatccccagc agtccggcgg tgtcttctga attgcccggg
     3421 agcatgtccg gcgtgatggc cagccgcagg ttggagacat ggcgcttggc cttgcccacc
     3481 agcgtggtgg ccttgatcag cggctccgcc gtcagcatgt aatagagaag catgccgagg
     3541 ctgtagatca cgctgggcgc atcctcgccc cggcggtaaa tgcgctccgg agggatgaat
     3601 tcagcggttc cgtccacata ggtttccgtg cctttccgcg acgcttcttc ccgcggaagc
     3661 gtgaggccga aatcaatcag caccgcatgc ccgttgggct ggacaatgac attcccggcg
     3721 ctgagatccc ggtaaagata gccggctttg taaatatgct tcatggccga tatgacctgc
     3781 gcaaggatat ccagcgcccg cacttcgtcc agcttgcctt tgtgggagag aatatgcttg
     3841 aggccctcgc cctcgatgaa atccatgatg aagtaatgat aatcttcttc aattccatgc
     3901 ccgtagaacg tggcgatgtt cggatggttg ttcacatcct catgaatgcg gacttccagc
     3961 aggaaattat caagtgtctc ccggtcccgg gcccgttcag gctggaggat cttggcggcg
     4021 aagagatgct ccgtgtcatc cttgcgccag gccttgtaga cgctggcatt gcctccggcg
     4081 ccgacgggct gaaagagcat gtagtcaccc atgcgcatcg gcacgaaatt cttgaacgcg
     4141 catttggggc attccgtgaa ggaaaacggc ttcagcccgg ccagagacag gacatggccg
     4201 cactgctcgc aaagcatgct gcctgattca aaaaattctt tgacggaacg cttctggcgt
     4261 ctttggtcca tgaagcgacc cacgaactca ccgaccttca taagttcctc tggtcatttt
     4321 cagtctctgt tatattaccc atgacacaga gtccgttcaa gaactccttt tcattatcgg
     4381 ccgcagcggg cacatgaagg aaaaccttgt ccttcaggcg ggctttggcc gggatctcca
     4441 gcgtaaccac gtcccccttc acggcccggg ccgccggccg gccgtccact cgcagatgct
     4501 gaacgatctg ccggatcgcg ccggttgtgg gcccctcgac cagcacaaca gcccccggtt
     4561 tcagctcacg ctgcatcatc gtgaactgca tcacccgcgg cgcggggtag aaatgctcga
     4621 ccagcccgat ctgaacgcgc ttcacggacg cctgcgaatt gcccatcccg ctccattcgc
     4681 ccagcctctc tccgcagtag tatccgccct cccagaaacc ccggttgaac acggtttcga
     4741 gacgggccat ccatccgtct gcgcgggcag cagtccatga accctctttc agcgcgtcga
     4801 gcgcctcgcg gtaaaccgcg gtcacggtc
//
LOCUS       DAOQWR010000166         4791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18054_length_4791_cov_4.328125, whole genome shotgun sequence.
ACCESSION   DAOQWR010000166 DAOQWR010000000
VERSION     DAOQWR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18054_length_4791_cov_4.328125"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKK36_10735"
     CDS             <1..891
                     /locus_tag="PKK36_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR95068.1"
                     /translation="GNQLVSRPFHEVVAHAGDLPLDMFTTSDPPGTPGILTFSRLLSK
                     TPFVVDMQALMRTLESAYGCPVDIEFAANFTEKDKYKINLLQCRPLQVQGAGTVEVPR
                     VHVTEQDRIIEARGAVVGHSRTGQIGRFIYVAPELYGKLPVADRYEVARIIGVLNLAD
                     APGPVMLLGPGRWGTSTPSLGIPVSFSEINRVSVLCEIVAMHEHLVPDVSLGTHFLNE
                     LVETGMLYLALFPGQGVNFISRAFFESAPDRAPALVPGAARWPGLIRVVDVTDIRPPV
                     RVNIIADAPNQRVICYMDRT"
     gene            complement(943..1035)
                     /locus_tag="PKK36_10740"
                     /pseudo
     CDS             complement(943..1035)
                     /locus_tag="PKK36_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
     gene            1435..2091
                     /gene="nth"
                     /locus_tag="PKK36_10745"
     CDS             1435..2091
                     /gene="nth"
                     /locus_tag="PKK36_10745"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNR95069.1"
                     /translation="MELKKLVWVHEKLARVYGPVQCRPHSDPLEQLIQTILSQNTSDI
                     NSDRAWRSLKKAFRTWKEVLDAPESKVRHSIRKGGLAGIKAPRIQNTLRIIREREHKL
                     SLARLRKMSSEEVMAYLTTIPGVGVKTAACVLLFSMGRPVMPVDTHVHRVTKRLGWAP
                     QNASPEDIGPMIEKKLPPELILPMHLYLVEHGRRICRARNPLCAECPLAAKCERLNLQ
                     "
     gene            2151..4121
                     /locus_tag="PKK36_10750"
     CDS             2151..4121
                     /locus_tag="PKK36_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNR95070.1"
                     /translation="MKNIIRIRPLTGFLGLLLLIFAAGGISAADLVVSFSAAGPDVFR
                     VTVSARAADEAPAPCRSQNTEMRFEDGMLLVERTDIPEAELLWSGRLIGLQAYGRANV
                     PGTRLEWKAHPGEAIYGLGQRFNALNQAGKMVEMWNRDAPGQGRENEASYFCTPVLFS
                     SAGYAFFAADNPDGAFSLNPLGEGLNRYSRAGQEWTFYLAFARTVKELVLQRARLQGP
                     FRGIPDWAWGPWISRNSYENQNEAEEAIKGMAERGFPVAAIVQEAWKGPYETGDFNNF
                     VKDRWPDLDRYFALCSQHDIRTVLWQVPVIPPSHPGLDEMAAKNYFVKNPDGAVSWRR
                     EWLEGMANIDFTNPEAVKWWKDQMRDEVRMGVRGFKADDGEDIKPDDVFADGRHGWQL
                     HNDYAALYAYALYELFDEEGADGMLWGRSASLGTERTPALWAGDQFASWEQYRSLLPA
                     GLSAGLSGAPFWGHDIGGYIGDPGPELYIRWLQFGAFSPLMQYHGVQRREPWEFGPEA
                     EAAYRLLSHLRMNLKPALIELGREAAETGLPVMRPMIMEFPDDPRFSSEDSQYMLGPN
                     LLVAPILEAGATARRVKFPAGRWRHLLRPDVFEGPAETSVVCGLVDAPVFVREGASLK
                     VQLAEGAKLGVWTLDAPVREIVIGPPLSAEAE"
     gene            4188..>4791
                     /locus_tag="PKK36_10755"
     CDS             4188..>4791
                     /locus_tag="PKK36_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95071.1"
                     /translation="MLTEHIKEALSQVKELQRMVMERQHFRGYSGWARIVSGTLALIA
                     SAIMASRFYPQNTRSHILGWGALFVVALVLNGAALVYWFFHDPKVGRDIARLKPLFDS
                     LPALAVGGALTAAMLMNDTHQYLFGIWMCMFGLSNLGSRYVLPRMIMLVGIFYIVSGI
                     AWLLAPSVDFLNPLPMGIVFFAGEWAGGLILHMDERRYILI"
BASE COUNT         1009 a         1262 c         1433 g         1087 t
ORIGIN      
        1 ggcaaccagc tggtttcacg tccttttcat gaggtggtgg cccatgccgg cgacctccct
       61 ctggacatgt tcacaacatc tgatcctcca ggaacgcccg gcatattgac tttcagccgc
      121 ctgctttcaa agacgccttt tgtggtggac atgcaggccc tgatgcggac actggagtcc
      181 gcatatggtt gtccggtgga cattgagttc gcagccaatt tcacggaaaa ggataaatat
      241 aaaatcaacc tgcttcagtg ccgccctctc caggtccagg gagcgggcac ggtggaggtt
      301 ccccgggttc atgtgactga acaagatcga attattgagg cccgcggcgc tgttgtgggt
      361 cacagccgga cgggccaaat cgggcgtttc atttatgttg cacctgagct atacggcaaa
      421 ctgccggtcg ctgatcgcta tgaggtggcc cggatcatag gagttctcaa cctcgccgat
      481 gccccgggcc ccgtcatgct tcttggcccc ggtcggtggg gcacgagcac tccatctctc
      541 ggcattcctg tatcattttc agagatcaac cgggtgtcgg tgctttgcga aattgtggcg
      601 atgcatgaac atcttgtgcc agacgtttcg ctgggcacac atttcctgaa cgaacttgtc
      661 gagacgggga tgctgtacct tgcactgttt ccggggcagg gggtaaattt catcagcagg
      721 gccttctttg agtccgcgcc ggatcgtgcg cctgctcttg tgcccggcgc cgcccgatgg
      781 ccgggcctga tccgtgtcgt tgatgtgacg gatatccgtc cgccggttcg ggtgaacatc
      841 atcgcagatg ctccaaacca gagggtcatc tgctacatgg accggacctg accgcaaagg
      901 ccttctgcgg aggctgccgc ttttgtccct gctccctccc ctttatccaa tggcctcgcg
      961 gccgtcttcc ccggtgcgga tgcggataca gtccaccaga tcaatcacca cgaatatttt
     1021 cccgtcgccg gtgtgacggg ggctgttcgg ctctgcacct tccgcttctg cgggacaagc
     1081 ccgcagaggg ccaagaaagg tccaaaagcc tgaaaactgg ccccaaaacg tcaaaaatga
     1141 ccagaaaatc gcaaaaaatc aggaaaatcg gcttaaaatg gtaaaaaacg tgaaaaaagg
     1201 gctgaaaacc gtgtgtttgt caaaatagta ctaaattagt actatttcaa aaaatgaacg
     1261 gccgggctcg gtagaagtgc tgaaagtgac gaaaaattta aaaaaatggt cacaaaacgc
     1321 tcaaaaatgc ccgaaaatgc ctaaaaatgg ccaaaaagcg atataaatac ccatgatttt
     1381 gcggattctg attttttcgt agattcagat atattttgtg ggaataatag atacatggaa
     1441 ctcaaaaaac ttgtttgggt gcatgagaag ctggcgcggg tttatgggcc ggttcaatgc
     1501 cggccgcaca gcgatccgct ggagcagctc atccagacga tcctttcgca gaacacatcg
     1561 gacatcaatt ctgatcgcgc gtggcgcagc cttaagaaag cctttcgcac ctggaaggaa
     1621 gtcctggatg cgcctgagag caaagttcgg cacagtattc ggaagggagg acttgccggc
     1681 atcaaggctc cgcgcattca gaacaccctg cgaatcattc gcgagcgcga gcataaactg
     1741 agccttgcgc ggctcaggaa aatgagcagc gaagaggtaa tggcatatct cacaaccatt
     1801 cccggcgtcg gagtcaagac agcggcctgc gtgctgctgt tcagcatggg gcgtccggtc
     1861 atgccggtgg atacgcatgt gcatcgcgtg accaaacggc tcggatgggc tccgcaaaat
     1921 gcctccccgg aggatattgg ccccatgatt gagaaaaagc tgcccccgga acttatactg
     1981 ccgatgcatc tttatcttgt ggagcacggc cggcggattt gccgcgctcg caatccgctc
     2041 tgcgccgaat gcccgctggc ggcgaaatgt gagcgactga accttcaatg aactggtcgc
     2101 aatggcttcg cgcttgccgc ggcgaagacg atgatcaata attctgagcc atgaaaaata
     2161 taattcgcat ccggccgctt acggggtttc tcggactcct gctcttgatt tttgcggcag
     2221 ggggaatttc agcggccgac cttgtggttt ctttttccgc ggcgggaccg gatgtgttcc
     2281 gggtgacggt atcggcgcgg gccgcggatg aggccccggc tccgtgccgc tcgcaaaata
     2341 ccgagatgcg attcgaagac ggcatgctgc tcgtggagcg cacggatata ccggaagctg
     2401 aactgttgtg gagcgggaga ctcattggtt tgcaggccta tggtcgtgcc aatgtgccgg
     2461 gtacccggct ggagtggaag gcgcatccgg gagaagcgat ttatggcctc ggccagcgct
     2521 tcaacgcgct gaatcaggcc ggaaagatgg ttgaaatgtg gaatcgcgat gcccccggcc
     2581 agggccgtga gaatgaggcc agttatttct gcacacctgt ccttttcagc agcgcaggat
     2641 atgcattctt tgcggccgat aatcctgacg gcgctttttc gttgaatccg ctgggggagg
     2701 gcctgaaccg atactcgcgc gcgggacagg agtggacttt ctatctcgcc tttgcacgga
     2761 ctgtgaagga acttgttctg cagcgcgctc ggttgcaggg gcccttccgc gggattccag
     2821 actgggcctg gggaccatgg atcagccgca acagttacga gaaccagaac gaggcggagg
     2881 aagccatcaa aggaatggca gaacggggtt tcccggttgc ggcgattgtg caggaggcat
     2941 ggaaaggccc gtacgaaacg ggcgatttca acaatttcgt aaaagaccgc tggccggatc
     3001 tggaccggta ttttgcgctg tgctcgcagc atgacattcg caccgtgctc tggcaggttc
     3061 cggttattcc tccatctcat cccgggctgg atgaaatggc ggcgaagaat tattttgtaa
     3121 agaatccgga cggtgcggtg agctggagac gcgaatggct ggagggcatg gccaatattg
     3181 attttacaaa tccggaagcc gttaagtggt ggaaagatca gatgcgggat gaagtccgga
     3241 tgggcgtgcg cgggttcaag gccgatgacg gtgaagacat caaaccggac gatgtgtttg
     3301 cggacggtcg ccacggatgg cagttgcaca atgactatgc ggccctctat gcttatgcgc
     3361 tctatgagct ttttgacgaa gaaggcgcgg acggcatgct gtgggggcgg agtgcgagcc
     3421 ttgggaccga aaggactccg gctctctggg ccggcgatca gtttgccagc tgggaacaat
     3481 atcgttcatt gcttccggcg ggactgagtg cgggtttgag cggcgcgccg ttctggggac
     3541 acgacattgg gggatacatt ggggatcccg gtccggagct ttacatccgg tggctccagt
     3601 tcggcgcctt cagtccgctg atgcagtatc acggagtcca gcgccgtgag ccatgggaat
     3661 tcgggcctga agctgaggcc gcctacaggc tgctttcgca tcttcggatg aatctgaaac
     3721 cggcgctgat cgaactgggc cgcgaagcgg ccgaaaccgg cctgccggta atgcgtccca
     3781 tgattatgga gttccctgat gatccgaggt tttcatcgga ggactcgcaa tacatgcttg
     3841 gcccaaattt gctggtcgcc cccatactgg aagcgggcgc aacggcgcgg cgcgtcaaat
     3901 ttcccgccgg ccgctggcgg catcttctcc ggcctgacgt ttttgagggt ccggcggaaa
     3961 cctccgtcgt ctgcggactg gtggatgcgc cggtttttgt gagagaaggc gcttcattga
     4021 aagttcagtt ggctgaaggc gcaaaactcg gcgtctggac tttggatgcc cccgttcggg
     4081 agattgtgat aggccctccg ctctccgcag aagctgaata aaaacatcaa ctttatattg
     4141 caaagtgctt tgcaatatgc ttcattatct caaaacaggc aggatttatg cttacagaac
     4201 acattaaaga agccctttcg caggtcaaag agctgcagcg catggtgatg gaacgccagc
     4261 acttccgcgg ctattcggga tgggcccgga ttgtgtcggg aacactggcg ctgattgctt
     4321 ccgccatcat ggcctcccgg ttctaccctc aaaacacccg ctctcatatt ctggggtggg
     4381 gggccctgtt cgttgtggcg ctggttctga atggcgcggc tctggtctac tggtttttcc
     4441 acgatcccaa ggtcggtcgc gacatcgcgc gcctcaagcc tctctttgac agtctgccgg
     4501 ctttggcggt cggcggggcc ctgacggcgg ccatgctgat gaatgacacc caccagtatc
     4561 ttttcgggat atggatgtgc atgttcggcc tttccaacct gggttcgcgc tacgtcctcc
     4621 ctcgcatgat tatgctggtc ggaattttct atatcgtcag cggcattgca tggctgctgg
     4681 ctccgagcgt tgatttcctg aatccgctgc cgatgggtat cgtgtttttt gccggagaat
     4741 gggccggggg gctgatcctt cacatggacg aacgccgtta catcctgatt g
//
LOCUS       DAOQWR010000167         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18300_length_4741_cov_6.615664, whole genome shotgun sequence.
ACCESSION   DAOQWR010000167 DAOQWR010000000
VERSION     DAOQWR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18300_length_4741_cov_6.615664"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..662)
                     /locus_tag="PKK36_10760"
     CDS             complement(114..662)
                     /locus_tag="PKK36_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNR95072.1"
                     /translation="MRKVLIAIAEGTEEMETVITTDVLRRAGWEIAIASISPAPVTCS
                     REVRIVPDCEWREADPLEYDALVIPGGAPGVERLMEDGRVLEALRIFDECGKWICAVC
                     AGPLVLQAAGILKNQRLTCHPAVKEDLHAGIWLDEPVVRDGHLITSQGPGTCFDFALS
                     IVSALENPDAARKIARGMVFEF"
     gene            764..1732
                     /locus_tag="PKK36_10765"
     CDS             764..1732
                     /locus_tag="PKK36_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95073.1"
                     /translation="MRLKIKQFMAVAGLAVMEAVRQPLCLLLLLTVVLFTGLLPFLIT
                     YSFGESGKLVRESGLALHMVSGLLMGAFLAGTAFEQYGKQDAAASILSKPIGRSLFFL
                     ARFTGIALAMLAYSAAVGIAVMLASRAAAAEYSTDWQAAGPLLSAPFCALLYAGINNY
                     FTRRPFVSFAWGLTLFALLAAAVIAACFPAEGMAGGLANTIPWNILPAIALSTLSILL
                     ITAIASAVATRAATLATLGLCLMIFLTGLISDYLYVRLGTDAFAGRAVHFLLPNWQHF
                     WAAEVLTEGGSIPWAYVRSVFYYTGLYLAGVLCLGTFLFRGVEIKA"
     gene            1732..3603
                     /locus_tag="PKK36_10770"
     CDS             1732..3603
                     /locus_tag="PKK36_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HNR95074.1"
                     /translation="MTKPQFKALWVAAAALAAGLAMDFYLAARSPLTALKALPGFLLI
                     SALVVLAAYVRQRLAWLAEIEKRDQALAASEIPQSAIFESDEDSEPFSISRSRQQFER
                     YVAPGIAPVLGMLQLYLAWRGWQKGEFPAVLPEQLPLAAAFLAGQAFVFFLMSRYLLG
                     VAREPGQERMKGPGIYLGLAALVSLLAVADALLMELGVLRADRVVSCFFILLLAVLGA
                     ENYIRAVAYLYSPRNAASPAPCVSYESRLGALLTDPASWVNNVAQALDYQFGFNISHT
                     WFYRFLENALAPLIIFQLAALYLLSCFVFLGVEEEGILERFGRPVPAAEGGLLASGVH
                     RKWPWPFETVRRFPSRRIQTLHIGNASRDYPAPMLWTVPHHSGQESFLTASRAATAST
                     RDDAQSVPVNLLSVHIPIEYRITNVYQYAFSAAEPLRLLEQSAYAGLTRITVGHGMEE
                     IISSGQIHFTQELKTFMQRRADEMELGVRIEFVGLRGVHPPVAVADAFESVVGALEER
                     ESTRLKASAYAARVLPLALADAEAAGEIAGAYRFRRRVLADAEAGQFTQRRKGYERAP
                     DVFRNDLYLRTLSGALSGARKYIVSADPGNQVIQFDFKEKLKPELFNFGSGLTEEFR"
     gene            3603..4499
                     /locus_tag="PKK36_10775"
     CDS             3603..4499
                     /locus_tag="PKK36_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNR95075.1"
                     /translation="MKHNIWVSILGLTVAAVFVLSMLLFSVREGEAVVLTTFGKPARD
                     AITEPGLRFRWPWPVQRVHRLDRRVQTLDGSFEQTLTRDGKSVIVMLYAGWQIGDPVA
                     FLERVGTPRDAERSLDGLLSNYKNAVIGQNDFAALINTDPEKLKIEEMEKQILEAVRP
                     EALERYGIEVRFLGIRRISLPEAITAKVFERMRAERSEMAEKYRSEGEGEAIRIRARA
                     DSRRDQVLAEADAAAKRLRAEGDAEAAEFYKIFEQDPELAMFLRKLEVLEETLKEKAT
                     VVLGTDTQPFDLLQGGPAIPAK"
     gene            4496..>4741
                     /locus_tag="PKK36_10780"
     CDS             4496..>4741
                     /locus_tag="PKK36_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95076.1"
                     /translation="MKSMAPEPHIMESGQAALNEALRISFRLLRAAMLLLLVCYLATG
                     IFIVRQHEEAFVLLLGRISGSGESRLLGPGLHWALPRP"
BASE COUNT          776 a         1453 c         1521 g          991 t
ORIGIN      
        1 gtttttttga cgatttttgg gaaaaaaccg tcgtccatca aaacaggaca aaattagtcc
       61 tgtatcagcg tccgatcgtc agtcaggtcg ctcttggtgc ggacgggttt catctaaaac
      121 tcaaacacca tgccgcgagc gatttttcgg gccgcgtcgg ggttttcgag cgccgagacg
      181 atggacagcg caaagtcaaa gcaggtgccg gggccctggc tggtgatgag atgtccgtcg
      241 cgcacgaccg gctcatcaag ccagattccg gcgtgcaggt cttcctttac ggccggatgg
      301 caggtgaggc gctgattttt caggatgccc gccgcctgca gcacgagcgg ccctgcgcat
      361 accgcgcaga tccatttccc gcattcgtcg aatatcctca aggcctcgag cacgcgcccg
      421 tcttccatca ggcgctcgac gcccggcgct ccgccgggga tgacgagggc gtcgtattcc
      481 agggggtcgg cttcgcgcca ttcgcagtcc gggacgatcc ggacctcccg cgaacaggtc
      541 accggcgccg gcgaaatgct tgcgatggca atctcccatc cggcgcggcg cagcacgtcc
      601 gtcgtgatga ccgtttccat ttcttcggtg ccttccgcta tggcgatcag aactttgcgc
      661 atggctttgc ctcctgaatc gaaactcgac ttcccgccgg gttgtccata aattgtgatg
      721 cgataacagt aactggaaaa agaacaaagg acaacgcgaa accgtgcgcc tgaagatcaa
      781 acagtttatg gccgtggccg ggctggccgt gatggaggcc gttcgccagc cgctctgcct
      841 gctcctgctg ctgacggtgg tgcttttcac cgggctgctc ccgttcctga tcacctattc
      901 attcggggag tccggcaaac tggtgcgcga gagcgggctt gcgcttcata tggtatccgg
      961 gcttctgatg ggtgcgtttc ttgcgggcac cgcctttgag cagtacggaa agcaggacgc
     1021 cgccgcctcg attctcagca agcccatcgg ccgttcgctg ttttttcttg cccgtttcac
     1081 gggcattgcg ctggccatgc tggcgtattc ggcggccgtc ggaattgcgg tgatgctggc
     1141 ctcgcgcgcg gccgccgccg agtacagcac ggactggcag gcggccggcc ctctgctgag
     1201 cgcaccgttc tgcgcgctgc tctatgcggg catcaacaat tacttcacgc gccgcccctt
     1261 tgtgtccttt gcgtgggggc tgacgctctt tgcgctgctg gcggccgcgg tgattgccgc
     1321 ctgtttcccg gccgagggca tggccggcgg gctcgcaaac accatcccgt ggaatattct
     1381 tccggccatt gcactgtcca cgctctcaat cctgctgatc accgcgatcg catcggccgt
     1441 tgccacgcgc gccgcgacgc tggccacgct gggcctgtgc ctgatgattt tcctgaccgg
     1501 cctgatcagc gattatctgt atgttcggct gggcacggac gcttttgccg gccgcgccgt
     1561 gcattttctg cttccgaact ggcagcattt ctgggcggcg gaggtcctca cggagggcgg
     1621 ttcgatcccc tgggcctacg tgcgatcggt attttattat acggggttgt acttggcggg
     1681 tgtgctgtgt ttgggtacct tcctctttcg cggagtggaa attaaggcgt aatgacgaaa
     1741 cctcagttca aggcgttgtg ggtggcggcg gccgcgctgg cggcgggcct cgcgatggat
     1801 ttttatctcg ccgcgcgttc tccgctgact gccctgaagg cgctccccgg ttttcttctg
     1861 atctcggcgc tggtggttct tgcggcctat gtccgccagc ggctggcgtg gctggccgaa
     1921 atcgaaaagc gcgatcaggc cctcgccgca tcagagatac cacagtccgc tatttttgag
     1981 tcagacgagg attcggagcc tttttcgatc tcgcgaagcc ggcagcagtt tgagcgctat
     2041 gtggcgcccg gcatcgcccc cgtgctgggc atgctgcagc tgtatctggc gtggcgcgga
     2101 tggcagaagg gagaatttcc cgccgtgctt ccggagcagc tcccgctggc ggccgcgttt
     2161 ctggcgggcc aggcctttgt ctttttcctc atgagccggt acctgctcgg ggtcgcccgc
     2221 gagccgggcc aggagcggat gaagggcccc ggcatttatc tgggccttgc ggcgctggtc
     2281 agcctgctgg cggtcgcaga cgccctgctg atggaactgg gcgttttgcg ggcagaccgg
     2341 gttgtgtcct gttttttcat cctgctgctg gcggtgctgg gcgcggaaaa ttatatccgg
     2401 gccgtggctt acctctacag tccgcggaac gcggcgtcgc ctgcgccctg tgtctcgtat
     2461 gaaagccgcc ttggggcgct gctgaccgat ccagcctcat gggtcaacaa cgtggcgcag
     2521 gcgctggact accagtttgg attcaacatc tcgcacacat ggttttaccg cttccttgaa
     2581 aacgcgctgg cgccgctgat catctttcag ctggccgcgc tctatcttct ttcctgtttc
     2641 gtctttctcg gggtggagga ggagggcatt ctggaaagat tcggccgtcc cgtccctgcg
     2701 gcggaaggcg gcttgctggc cagcggcgtt caccggaaat ggccgtggcc gtttgaaacc
     2761 gtccgccggt ttccctcccg gcgcattcag actctccaca tcggcaatgc atcaagggac
     2821 tatcccgcac ccatgctgtg gaccgttcct catcacagcg ggcaggagag tttcctgacg
     2881 gccagccgcg ccgcgacggc atccacgagg gacgatgcgc aaagcgtgcc ggtcaatctg
     2941 ctgtcggtgc atattccgat cgaataccgg atcaccaatg tttatcagta tgccttcagc
     3001 gcggcggaac cgctgcggct tcttgaacag tccgcgtatg ccggcctcac gcgcatcacc
     3061 gtcggtcacg gcatggagga aatcatcagt tccggacaga ttcatttcac gcaggagctg
     3121 aaaaccttca tgcagcggcg ggcggacgaa atggagctgg gtgtccgcat cgagtttgtc
     3181 ggcctgcgcg gggtgcatcc gccagtggcg gtggcggacg ccttcgagtc ggttgtcggc
     3241 gcgctggagg agcgcgagtc gacccggctg aaggcttcgg cctatgcggc acgggtgctg
     3301 ccgctggcgc tggccgatgc cgaggccgcc ggcgagatcg ccggcgcgta tcgttttcgc
     3361 cgcagggttc tggcggatgc cgaggccggc cagttcacgc agcgccggaa gggctacgaa
     3421 agggctcccg atgtcttcag gaatgacctt tatctccgca cgctgtcggg cgcgctgtcg
     3481 ggcgcccgga aatacattgt gagcgccgat ccgggcaatc aggtcatcca gttcgatttc
     3541 aaggaaaagc tgaagccgga actgttcaat ttcggttcgg gcctcacgga ggagttccgc
     3601 tgatgaaaca caacatatgg gtatccatcc tgggcttgac ggtcgcggcc gtgttcgtgc
     3661 tgagcatgct gctcttctcc gtgcgcgagg gcgaggccgt tgtgctcacc accttcggca
     3721 agcctgcgcg ggacgccatc accgaacccg gcctccggtt ccgctggccg tggcccgtgc
     3781 agcgggtgca ccgcctggac cgccgcgttc agacactgga cggttcgttt gagcagactc
     3841 tcacgcgcga cggaaaaagc gtcattgtga tgctctatgc aggctggcag atcggcgatc
     3901 cggtcgcatt tctggaacgg gtcggtacgc cgcgcgacgc cgagcgcagc cttgacggac
     3961 ttctcagcaa ctataaaaac gccgtgatcg ggcagaacga tttcgcggcg ctcatcaaca
     4021 ccgatccgga aaagctgaag atcgaggaga tggagaagca gattctggag gccgtgcggc
     4081 cggaggcgct ggagcgctac ggcattgagg tccgcttcct cggcatccgc cgcatcagcc
     4141 tgcccgaggc catcaccgcc aaggtgtttg agcgcatgcg ggccgaacgc agtgaaatgg
     4201 ccgagaaata ccgctccgag ggcgagggcg aggccatccg catccgtgcg cgcgccgaca
     4261 gccggcgcga ccaggtgctc gcggaggccg atgccgccgc caagcgcctc cgcgccgagg
     4321 gcgatgccga ggcggccgag ttctacaaga tcttcgaaca ggaccctgaa ctggccatgt
     4381 tcctccgcaa gctcgaagtg ctggaggaaa cccttaagga gaaagccacc gttgtgctgg
     4441 gcactgacac gcagccgttc gatctgctgc agggcggtcc ggccatcccg gcgaaatgaa
     4501 atctatggca ccggaaccgc acatcatgga atcgggacag gccgcgctga acgaggcgct
     4561 gcggatcagc ttccgcctgc tgcgcgcggc catgctgctc ctgctggtgt gctatctggc
     4621 cacggggata ttcatcgttc gccagcacga agaggccttt gtgctgctgc tcggcaggat
     4681 cagtggaagc ggcgagagcc gtctgctggg gcccggcctg cactgggcgc ttccgcgccc
     4741 g
//
LOCUS       DAOQWR010000168         4724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18401_length_4724_cov_5.519169, whole genome shotgun sequence.
ACCESSION   DAOQWR010000168 DAOQWR010000000
VERSION     DAOQWR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18401_length_4724_cov_5.519169"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKK36_10785"
     CDS             <1..192
                     /locus_tag="PKK36_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95077.1"
                     /translation="ETPELVEAVRTGKNPSFALVKTHADIQIMLRSWEDQLNFEPSRR
                     FLTRKGGPKSVRPPAHSAR"
     gene            complement(189..1346)
                     /locus_tag="PKK36_10790"
     CDS             complement(189..1346)
                     /locus_tag="PKK36_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNR95078.1"
                     /translation="MKIFRWTGLFLVFGALASVYATEGINLIGIGPRQQGTAGAGVAA
                     AQDSTWIVLNPAGLANLEPGADVSFQLFGPSRTIRSTANPAAGKQKDDSVFYIPSISA
                     VFPLESSQNQVLGLGLYGSSGMGVDYDHPRVGFSDGDSRTELAIAKFTAAYARSFDSG
                     FSIGAGPVFVLGRFRTDMETSRIGPPSSPAGDDWDDAPGVGAIIGANQKINDRLRAGI
                     SYTSEQFMEEYGDYENLFLDSLNLPQQVTAGLAFRLTDDVELVLDYRWIGWGDLETFG
                     DQFGWEDQHIVKAGAIWQAGDQLTLRTGISHGNSPIGREDVFANALFPAIMETHVTAG
                     ATWRFERFALHAAFIHGIEEEITASGPPEAGGGTTVSMVQNSFTAGASWTF"
     gene            1595..2329
                     /locus_tag="PKK36_10795"
     CDS             1595..2329
                     /locus_tag="PKK36_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNR95079.1"
                     /translation="MKILHNIIDTLEAGDVPVKTVCTGAFWTAVVSKYGGLSSTYRDM
                     DPQHDDNPKFVEDAGNLHEKSALELARYALSDHTVAASIGMAAINSLIDVDESKCSNL
                     NAAEIVMGKGAGKNVAVVGHFPFIPKLRESAKRVWVIEKKPRPGERPAEEAAEILPQC
                     DVVCLTSSAFINHTIEDLLPLCGNSFVVLTGPTSPLTPILFDHGVDVICGARITNPER
                     VVQYITQGACFRQMKFAGVKLLSLAK"
     gene            complement(2332..3708)
                     /locus_tag="PKK36_10800"
     CDS             complement(2332..3708)
                     /locus_tag="PKK36_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNR95080.1"
                     /translation="MNEKFDVIVIGSGGGMKIARPAAQRGLKTALIERDAVGGTCLNR
                     GCIPSKMLIYPADLVHLMRRAPRVNLRGGEITADWPALIDRVSSTVDEMSRSQEKKLS
                     TMPNMTFIRGDAHFTGERELDVSGRALTADRIFIATGSEPDIPAVEGLEGTPCMTSRE
                     ALRRRALPKKLIVIGAGYIACELGHVYRAFGTETHFVVRSKVLRTAEPDVRKAFESAF
                     EKDHHLHEGWNVQRVGHASGMFSVSMTNRASEEKILEADALLVATGVRPSTGDLNLST
                     AGVCTDDRGFIRVNEYLETDAPGIYALGDCVGNYLFRHSVNLEGEYLVRTLWSGAVRR
                     PIDYGPMPHAVFTHPEIAGLGATEESLRETGVDYVIGRAEYRDSNQGLARGLDEGFCK
                     LLVARNDLRVLGAHIIGDEASDMIHMLAAVMYRGGTLDDLLNMVYIHPALPEIVRDAA
                     RDARARLG"
     gene            3801..4487
                     /locus_tag="PKK36_10805"
     CDS             3801..4487
                     /locus_tag="PKK36_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95081.1"
                     /translation="MNCELNGRSESALAVMRTARGLLCIFWGLPLSLLLFSQALTIRT
                     SFVLPAYLPGVMLICIGVIWLWSAGRMSPSWRPLVRQTIMILCLIIYFAPFVSWWEML
                     PATAYLLSNLWLFFLTVAGLLHLLNRLAGDVAEQTDCQSLALEARFCRRTVFLLMMLP
                     LVLIYLRAAAGALIHGGSLYSEIAQNLIALPSWTLLLFLFPFPMTMANLWRARNQCLG
                     MLDISRRKAE"
     gene            complement(4536..>4724)
                     /locus_tag="PKK36_10810"
     CDS             complement(4536..>4724)
                     /locus_tag="PKK36_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95082.1"
                     /translation="MATSEFRNKNLQRPKKSQNDQRKRLRVQQKRLIALGMAEAAVMK
                     MTHPQIRAALRKPAKIKK"
BASE COUNT          934 a         1458 c         1340 g          992 t
ORIGIN      
        1 gaaacgcccg agctggtcga ggccgtccgg accggaaaga acccgtcttt tgctcttgtt
       61 aaaacccatg cggacattca aatcatgctc cggtcctggg aagaccagct caatttcgaa
      121 ccgtcgcgca ggtttctgac caggaagggc ggaccgaaat cggtccgccc tccggcccat
      181 tccgcccgct aaaaggtcca gctcgctccg gcggtgaaac tgttttgcac catgctgacg
      241 gttgtcccgc ctccggcttc tggcggaccg ctcgccgtga tttcctcctc gatgccgtga
      301 atgaaggccg cgtggagagc aaagcgctcg aagcgccagg tggccccggc ggttacatgg
      361 gtttccatga tggccgggaa aagcgcgttt gcgaaaacat cctcgcggcc tatcggggaa
      421 ttgccatgcg aaatgccggt ccgcagcgtc agctgatcgc cggcctgcca gatggcgccc
      481 gccttgacga tatgctggtc ttcccagccg aactgatcgc cgaaggtttc gagatccccc
      541 catccaatcc agcggtaatc gagaacaagc tcgacgtcat ccgtgagacg aaaggccagt
      601 ccggcggtaa cctgctgcgg aaggttgagc gagtccagaa aaagattttc gtaatcgccg
      661 tattcctcca tgaactgctc gctggtgtag ctgatccccg cgcgcaggcg atcatttatt
      721 ttctggttcg cgccgatgat ggcgccgacg ccgggggcgt catcccagtc gtctccggcc
      781 ggcgacgacg gcgggccaat ccggctggtc tccatgtcgg tgcgaaaacg gccaagcaca
      841 aatacgggac ccgccccgat gctgaatccg ctgtcgaagg agcgggcata ggcggctgtg
      901 aattttgcga tggccagttc cgtgcgcgaa tctccgtctg aaaaacccac ccgcggatga
      961 tcataatcaa cgcccatgcc gctggaccca taaagaccga ggccgaggac ctggttctgg
     1021 cttgactcca gcggaaaaac cgcactgatg gatggaatat agaacaccga atcgtctttc
     1081 tgctttcccg cggcgggatt ggctgtggat cgaatcgtgc gggagggacc aaatagctgg
     1141 aacgaaacat ccgcccccgg ttcaaggttc gccagtccgg cgggattcag cacaatccac
     1201 gtggaatcct gcgcggccgc cacgcccgcg ccggcggtgc cctgctgccg cgggccaatg
     1261 ccgatcaggt taatgccctc cgtggcataa acggaggcca gcgcaccgaa aacaagaaac
     1321 agtcctgtcc atctgaatat tttcatcttt ctccttttca attagttata aacaaaccca
     1381 tcaaggtcta gtattactat gaatatagta gtatattgcg aggcacgcct tgtcaaggct
     1441 ctgattcgtt tttttcacaa acgccgggcg ccggcttcca ttgccgccat acaaacgaag
     1501 ggcccggaac cgataaagct tcacgcgggg aagcaatcag gatagcatgg ccccatcttt
     1561 cacagagccg cgtccgcatg caactggatg ggtcatgaaa atactgcaca acattataga
     1621 cacgctcgaa gccggcgatg tccccgttaa aaccgtctgc accggcgcgt tctggaccgc
     1681 cgtggtttca aaatacggcg ggctgtccag cacgtaccgc gacatggacc ctcagcatga
     1741 cgacaatccg aagttcgtcg aagacgcggg gaatctgcac gaaaaatccg cgctggaact
     1801 ggcgcgctac gcgctttccg accacaccgt ggccgcctcc atcggcatgg ccgcgatcaa
     1861 ctcgctgatt gatgtagatg aatcgaagtg ctcgaatctc aacgcggccg aaatcgtcat
     1921 gggaaaaggc gcgggaaaga atgttgccgt cgtcgggcat ttcccgttca tccccaaact
     1981 gagggagtcg gcgaagcggg tctgggtaat cgaaaagaag ccccgcccgg gcgaacggcc
     2041 cgcggaggag gccgcggaga ttctgccgca gtgcgatgtg gtctgcctga cttcgtccgc
     2101 attcatcaac cacaccatcg aagatctgct cccgctctgc ggaaacagct ttgtggtgct
     2161 gacgggcccg acctcgccgc tgacaccgat cctgtttgat cacggggtgg atgtgatctg
     2221 cggcgcgcgc attaccaatc ccgaacgggt ggtgcaatac atcacccagg gagcctgctt
     2281 ccggcagatg aaattcgcgg gcgtgaaact gctgtcgctt gcgaaataat ttcagcccag
     2341 ccgggcgcgc gcgtcgcggg ccgcatcgcg cacaatttcc ggcagcgcgg gatggatgta
     2401 aaccatattc agcaggtcat ccaaagtgcc gccgcgatac atcaccgccg caagcatgtg
     2461 aatcatgtcc gaagcctcat cgcctataat gtgcgcaccc agcacacgca gatcgtttcg
     2521 ggcgaccagc agtttgcaga aaccctcgtc cagaccccgc gcaagtccct ggttgctgtc
     2581 ccggtattcc gcccgaccaa tcacataatc cacacccgtc tcccgcaggc tttcttccgt
     2641 ggctccgagg ccggcaattt cagggtgcgt gaaaaccgca tgcggcatgg ggccgtaatc
     2701 aatcgggcgg cggacagcac cgctccagag ggtgcgcaca agatactcgc cctcaaggtt
     2761 gacgctgtgc cggaacagat aatttccaac gcagtccccc agcgcataaa tccccggggc
     2821 gtccgtttcc agatattcgt tgacgcgaat gaacccccga tcgtccgtgc aaacgccggc
     2881 ggtggacagg ttgagatccc ccgtggaagg ccgaaccccg gttgcaacca gcagggcgtc
     2941 ggcctccaga attttctcct ctgatgcgcg gtttgtcatc gagacagaga acattccgga
     3001 ggcatggccg actctctgca cattccatcc ttcgtgaaga tgatggtctt tttcaaaagc
     3061 ggattcgaat gccttgcgta cgtccggctc cgccgtgcgg agaactttac tgcgcacaac
     3121 aaaatgcgtt tctgtgccga aggcgcgata gacatgcccc agttcgcagg cgatgtagcc
     3181 ggcccctatg actatcagct ttttcggcag ggcgcgccgg cgcagcgcct cgcggcttgt
     3241 catgcagggc gttccctcaa gtccctcgac cgcggggatg tccggttcag atcctgtggc
     3301 aatgaatatc ctgtccgccg tcaaagcccg gccgctgaca tcaagttccc tctcgccggt
     3361 aaaatgcgcg tcgccgcgaa taaatgtcat gttcggcatc gtgctcagct tcttttcctg
     3421 gcttcgggac atttcgtcca ccgtggacga aacacggtca atcagcgccg gccagtcggc
     3481 ggttatttcg ccgcccctca gattcacccg cggcgcgcgg cgcatcagat gcacaaggtc
     3541 cgccgggtag atcagcattt tggatgggat gcagccgcgg ttcaggcagg ttccgcccac
     3601 cgcatcgcgt tcgatcaacg cggttttcag gccccgctgc gcggcaggac gcgctatctt
     3661 cattccgccg ccggagccaa ttacaatgac atcgaatttt tcattcatgc ggacagtcta
     3721 gcggatcgcc gcaaaagttc caatcattgg aacttttcat cgggcttgcg ccggccgcat
     3781 ccgggagcta ataatgccgc atgaattgtg aactgaacgg ccgctctgaa tccgcactgg
     3841 cggtcatgcg caccgcgcgc ggactgctgt gcattttctg ggggcttccg ctgtcactgc
     3901 tgctcttttc gcaggccctc accatccgca caagctttgt cctgccggcc tatcttccgg
     3961 gcgtgatgct gatttgcatc ggagtgatct ggctctggtc ggcgggccgc atgtcgccgt
     4021 cgtggcggcc gctggtcagg cagaccatta tgattctctg cctcatcatc tattttgcgc
     4081 cgtttgtgag ctggtgggaa atgctgcccg cgacggccta tctgctcagc aatctctggc
     4141 tgttttttct gactgtggcg gggcttctgc acctgctcaa ccgcctggcg ggcgatgtgg
     4201 ccgagcagac cgactgccag tcgcttgcgc ttgaagcgcg cttctgccgc agaactgtgt
     4261 ttctgctgat gatgctccct ctggtgctga tttatctgcg cgcggcggcc ggcgcgctga
     4321 ttcacggcgg atcgctctac agcgaaatag cgcagaacct gatcgcgctc ccgtcctgga
     4381 ccctcctgct gtttcttttc ccctttccca tgaccatggc caacctgtgg cgggcgagga
     4441 accagtgcct cggcatgctg gacatcagcc ggcggaaagc ggaataaaaa agccgccggc
     4501 tttcaccggc ggcttcttca taaacagcaa aacggctatt ttttgatctt cgcgggcttg
     4561 cgcagcgcgg cgcggatctg cggatgagtc attttcatca ccgcggcttc ggccatgccg
     4621 agcgcgatca ggcgcttctg ctgcacgcgc agacgcttcc gctggtcatt ctggctcttt
     4681 ttgggccgct gcaggttctt gttcctgaat tcactcgttg ccat
//
LOCUS       DAOQWR010000169         4695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18543_length_4695_cov_10.613793, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000169 DAOQWR010000000
VERSION     DAOQWR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18543_length_4695_cov_10.613793"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKK36_10815"
     CDS             complement(<1..284)
                     /locus_tag="PKK36_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95083.1"
                     /translation="MAAKYDQQKARQVLEESRWQSFQSSDPALLNDGEVVRVIRNVDR
                     KNVDDCVQFLENYTSSLNAKPVHSVTDPLIDNRPRRGLWRCVDVISREEWS"
     gene            complement(289..1122)
                     /locus_tag="PKK36_10820"
     CDS             complement(289..1122)
                     /locus_tag="PKK36_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95084.1"
                     /translation="MMVQLGQLKEQLQAATDYTTNTEIATLMSALNMAYYDLAGSASW
                     VALRRKIALVSIPTGGYWLPGNLAGIDRIWDADGNDYPIRELGNTFNESDARYRAVIS
                     EVCREPLVYQRGVSIDSGTKALAFDPAISQATIGEYIAFGKQLGCYYVETADTLELTY
                     YGPTINNEFFQIRPIGTKKLKLIDYNGDDLAATVNIAYWQYPPPLYADHEIIQLPTTE
                     ALFYKTLMRLNQFKRNEGRAKNLQNDYAVAYSDMLARNPAYEVFNAPAFRTGEEMTWG
                     R"
     gene            complement(1179..1493)
                     /locus_tag="PKK36_10825"
     CDS             complement(1179..1493)
                     /locus_tag="PKK36_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95085.1"
                     /translation="MRLPFTKRKKYRLPYTIKYYSVKYAERVTVPEGYESDGATGATD
                     IWTESFWVHDLLCERHTWDSGEPCTSKQAAEVLADILWREKRYLRSLVWYLAVRCFGP
                     RF"
     gene            complement(1490..1708)
                     /locus_tag="PKK36_10830"
     CDS             complement(1490..1708)
                     /locus_tag="PKK36_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95086.1"
                     /translation="MKRNKTDSTGSFWITCALAYAASLALWLCACNYFPSATHYKCTI
                     IGDPVSSEILVEGAGGASMDGTLKASAK"
     gene            complement(1710..2651)
                     /locus_tag="PKK36_10835"
     CDS             complement(1710..2651)
                     /locus_tag="PKK36_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95087.1"
                     /translation="MNGLFAKTVEMRQKMADYTKTWWGRIYLAWKQFRAEMKSGGAEK
                     PEQEQGETEQGGAEILQPSTPSDIHRASFLFDNAGTRCMNAFGMPEQKFKETVERCRK
                     NGDNIYYAFLNNEHDGNHAGFSPYKDNKIGGEIDRSKVSMMQDRIKYIRENGMAVVLW
                     LRADDSPSFNRASEDAQKTMQSHAAQYFDGLVDGYCLALEWNEYEGDTSRMHRYAAHM
                     RQHTDADIGVHFTSGNKASLANKILRAVSGSRTYYLQYGFGKNTGYVGELTRQVRAEL
                     DAHIRLIGCEYNKNSDRKDYAEAILANGAAGYGNGRA"
     gene            complement(2801..3094)
                     /locus_tag="PKK36_10840"
     CDS             complement(2801..3094)
                     /locus_tag="PKK36_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95088.1"
                     /translation="MPDNNHINFEDCPVGIRVLQLLENVTAELRSSKEEIAKLNKRLF
                     YNNGGKCLQTIVAESTGKVNAHDRILWGIGGGIGLILITQLMMIILKFSGAGQ"
     gene            complement(3087..3539)
                     /locus_tag="PKK36_10845"
     CDS             complement(3087..3539)
                     /locus_tag="PKK36_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95089.1"
                     /translation="MIWLLGILAAAGGGFAVWKHNQKDESASGWSVKVQKGYEAQWER
                     CFARAGVLLAPLGLSKPRGSLTVKAAPGDGQVGGHWGKLKPGGAITGAYTARSGSRQT
                     VQLYTDPQGRETDGEMIHEAAHVRLNCDKRYRGADQHSIMKKYGVPHA"
     gene            complement(3536..>4695)
                     /locus_tag="PKK36_10850"
     CDS             complement(3536..>4695)
                     /locus_tag="PKK36_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95090.1"
                     /translation="AITGLTASAGTDTGAVQKVGDTMTGPLDISPSGTNCLKLTSSIT
                     HTNIEIGYGATSDFGDFGSIAIGYLANAGFQGGPGENIAIGREALGTRNAVAIGNGSY
                     AYLGSVAIGNKANGYNFGSAVGYYANGYNGSAVGHYANGASYGAAVEYAANGARDGAA
                     VGYGANGSGTNIAIGYGAKANGGLYRSAIGHSVSNSVDNSAAIRGSLYLDGGTGIFFR
                     TTFGTGDWGPALIVKSMSGTNELVAVDPDGNETQLSAHDVIDGKMVSVAKSHNTFTGR
                     GIIIDLEAMAREVEKLSGAKIVHEYQVPKADWNLVEAQKEAKRIAARAAWSNQFAAFS
                     AKPLTLITNIVDGVEEVKEEGTPDPGPIPPTYKPRPKPDWLTAAESVGVEK"
BASE COUNT          982 a         1337 c         1160 g         1216 t
ORIGIN      
        1 gaccattctt cgcgggaaat gacatcaacg catcgccata gtccgcggcg ggggcggttg
       61 tctatcagtg gatcggtaac agaatgaacg ggttttgcat tcagggatga cgtatagttt
      121 tcaagaaact gtacgcaatc atccacattc ttgcggtcca cgttccgaat aacgcgaacc
      181 acttctccgt cattcagcaa cgccggatcg ctggactgga aggactgcca gcgcgattcc
      241 tcaagaacct gtcttgcctt ctgctggtca tattttgcgg ccatgatatt atctccccca
      301 agtcatttct tcgcccgtcc tgaatgccgg ggcattgaat acctcgtatg ctggattgcg
      361 ggcgagcata tctgaatacg caacggcata gtcattttga aggttctttg ctcgtccctc
      421 attacgctta aactgattca ggcgcatgag cgtcttgtag aacagggcct cggtcgtcgg
      481 aagctgaatg atttcgtgat ccgcatacag ggggggcgga tactgccagt aggcgatatt
      541 cacggtggcc gcaaggtcat ccccattgta gtcaatgagt ttcagcttct tcgttccaat
      601 gggcctgatc tgaaagaact cgttattgat cgtcggacca taatacgtca attccagggt
      661 gtctgcggtt tctacatagt agcaacccaa ctgcttccca aaagcgatgt attcgccgat
      721 tgtggcttgt gatatggccg gatcaaacgc aagagccttt gtcccgctgt caatgctcac
      781 ccctcgctga taaacaagtg gctcacggca gacttccgat atgactgcgc ggtatctcgc
      841 atcagactca ttgaaggtgt tgccaagttc tcggatggga taatcatttc cgtccgcatc
      901 ccaaattcga tcaatcccgg caaggttgcc cggaagccag tatccgccag tggggataga
      961 cacaagggct atttttcgac gcaaggcaac ccaactggca gaaccggcaa gatcatagta
     1021 cgccatattg agcgcactca taagcgttgc tatttccgtg ttcgtggtgt aatcggttgc
     1081 cgcctgcaac tgctctttca actgacctaa ttgtaccatc atttcaaatc tcccttctta
     1141 aaccaccatc aacgcattca aattacatac acaaaagcct agaacctcgg cccgaaacag
     1201 cggactgcaa gataccagac cagcgatctt aaatatcgct tttcacgcca gaggatgtca
     1261 gccaacactt cagctgcttg tttgcttgtg cagggttcgc cggagtccca tgtatgccgt
     1321 tcgcacaaca gatcatgcac ccaaaaagat tccgtccata tatcagttgc accggtcgcg
     1381 ccgtccgatt catagccctc cggcaccgtg acgcgctcag catatttcac gctgtaatat
     1441 ttgatcgtgt aaggcagccg gtactttttg cgctttgtaa acggcagtct cattttgcgc
     1501 tcgccttcag ggttccgtcc atgctggccc cgcctgcgcc ctctacaaga atctcactcg
     1561 acacagggtc gccaatgatt gtgcatttgt agtgcgtcgc gctcggaaag tagttacagg
     1621 cgcacagcca gagcgcaaga ctcgcggcat aggccagagc gcaagtaatc caaaatgatc
     1681 cagttgaatc tgttttgttc cgcttcattt cacgccctcc cgtttccgta cccagccgcg
     1741 ccgttagcca ggatagcttc ggcgtagtcc ttgcggtcag agtttttgtt gtactcgcat
     1801 ccgatcagcc ggatatgggc gtcaagctct gcgcgtacct gtcgtgtaag ctctccgaca
     1861 tatccggtat tttttccgaa gccgtattga agataatatg tacgcgatcc agatacagca
     1921 cgaaggattt tgtttgcgag actggccttg ttcccgctgg tgaagtgtac gccaatatct
     1981 gcatccgtgt gctggcgcat gtgcgctgcg tagcggtgca tccgggacgt gtctccttca
     2041 tattcattcc attcgagcgc gaggcaatac ccgtcaacaa gcccgtcaaa gtattgcgct
     2101 gcgtgggact gcattgtttt ctgcgcgtcc tcactggcgc ggttgaatga tggagagtca
     2161 tcggcacgaa gccaaagcac gaccgccatg ccgttttcgc ggatgtattt gatgcggtcc
     2221 tgcatcattg aaacctttga gcgatcaatt tcgccgccga tcttgttgtc cttgtatggg
     2281 ctgaatccgg cgtggtttcc atcgtgttca ttgttcaaaa aggcataata gatattgtca
     2341 ccgttttttc ggcaacgctc aaccgtctct ttgaacttct gttcaggcat cccgaaggcg
     2401 ttcatgcacc gtgttccggc attgtcgaaa agaaaagatg cgcggtgaat gtcggacggc
     2461 gttgatggtt ggaggatttc ggctccgcct tgttcggttt cgccttgttc ttgttcaggt
     2521 ttctccgcgc cgcccgactt catctcagct ctaaattgtt tccatgccag ataaatccgg
     2581 ccccaccatg tttttgtata atcagccatt ttctgcctca tctctacagt cttcgcaaat
     2641 aatccgttca tgcgccttca gcaccgcgcc gcagagcttg cacagcctca cgttgtcttt
     2701 ttcctcactc ataatgcaat catcatttta aggacaatca caaacagcgc aagaataacg
     2761 atgcacatga cgaagtccac agaatctatg ccgaagatta tcattgtcct gcaccgctga
     2821 atttcagaat aatcatcatt aactgcgtaa tcagaatcag cccgatcccg ccgccgatac
     2881 cccacaaaat gcggtcgtgc gcattaacct ttcccgtgga ttctgcgaca atggtttgca
     2941 gacattttcc gccgttgttg tagaaaagcc gcttattcag ctttgcaatt tcttccttgc
     3001 ttgaacgcag ttctgccgtg acgttttcaa gtagctgcaa aacgcgaatc cctacagggc
     3061 agtcctcgaa attgatgtgg ttgttgtcag gcatgaggaa ctccgtattt tttcatgatg
     3121 gaatgctggt ctgcaccgcg atagcgtttg tcgcagttca ggcggacgtg cgcggcctcg
     3181 tggatcatct cgccgtcggt ttccctgccc tgcgggtctg tgtagagctg gaccgtctgg
     3241 cgcgatccgc tgcgggcggt gtatgcgcct gtgattgcgc cgccgggctt gagcttgccc
     3301 caatgcccgc cgacctgccc gtcgccgggg gcggccttca ccgtgaggct tccgcggggc
     3361 ttggagaggc ccaggggggc cagcaggaca ccagcacggg caaagcaccg ctcccactgc
     3421 gcctcataac ccttctggac cttcacggac cagccggatg ccgattcgtc tttctggttg
     3481 tgcttccaga cggcaaatcc gccgccagcc gccgctagga tgccgaggag ccagatcatt
     3541 tttccacccc cacggattca gcggctgtta gccagtctgg tttagggcgc ggtttgtagg
     3601 tcggcgggat cggtccgggg tctggcgtcc cttcttcttt aacttcctca accccatcca
     3661 ctatgttcgt gattaaggtc aatggcttcg cgctgaatgc ggcaaactga ttcgaccatg
     3721 cggcgcgggc agcgatcctc ttcgcctctt tttgagcctc gaccagattc caatcagctt
     3781 ttggcacctg atattcgtgg actattttcg ccccggacaa cttttccacc tctctcgcca
     3841 tagcctccaa atcaatgatg attccgcgcc cggtgaaggt gttgtgcgat ttcgcaacgc
     3901 tcaccatttt cccgtcaatg acatcatggg cggaaagctg tgtctcgttt ccgtccgggt
     3961 caaccgcaac cagttcgttt gttcctgaca tggatttcac gatcagcgca ggtccccaat
     4021 cgcctgtgcc gaaagttgtg cggaaaaaga tgcccgtccc accatccaga tacagcgagc
     4081 cgcgaatggc ggcagagttg tccacggagt ttgatacgct gtggcctatg gcggaacggt
     4141 ataacccgcc atttgctttt gccccgtatc cgatggctat gttggtgcct gatccatttg
     4201 ccccgtaccc aactgccgca ccatcgcggg caccgtttgc cgcgtactca actgccgcac
     4261 catagctggc accgttcgca tagtggccga ccgccgaacc gttgtaaccg ttcgcatagt
     4321 agccgactgc cgaaccaaag ttgtaaccgt tcgctttgtt gccgatcgcg acggaaccaa
     4381 gatatgcgta agagccgttt ccgatggcaa ctgcattcct tgttcccaaa gcctctctcc
     4441 ctatggctat attttcacca ggccctcctt gaaaaccagc attggctaaa tatccaattg
     4501 caatagagcc gaaatccccg aagtcactag ttgctccata accaatttct atattcgtgt
     4561 gcgtaatgct ggaagtcagt ttcaggcagt ttgttccgct tggtgaaata tccagcggcc
     4621 ccgtcatcgt gtcgccaacc ttctggaccg cgccggtgtc tgttcctgcg gaagcggtta
     4681 gcccggtaat cgccg
//
LOCUS       DAOQWR010000170         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18675_length_4674_cov_5.638017, whole genome shotgun sequence.
ACCESSION   DAOQWR010000170 DAOQWR010000000
VERSION     DAOQWR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18675_length_4674_cov_5.638017"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKK36_10855"
     CDS             <1..493
                     /locus_tag="PKK36_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HNR95091.1"
                     /translation="VDWSKTQAYSFGLSGIYLNRRGRESQGIVDDAGVRRLKEDIAAR
                     LKLEDAVLQVYDADAVYRGPYVKNGPDLIVGFREGWRASWESAVGTVRTEVVTQNTKV
                     WQGDHCVDQSLVPGVFFCSRRLADDRPLSIADVAPTVLEALGVPVPAYMDGCAVTLKS
                     GRQ"
     gene            508..2505
                     /locus_tag="PKK36_10860"
     CDS             508..2505
                     /locus_tag="PKK36_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNR95092.1"
                     /translation="MSSRQNKGLYTRRDFLKTAGAAALAMGVAGCPAVVRPSKGRNRI
                     IVLAVDGMDPRLMLDFIRAGGMKNCKALMEQGCFYPLRSSDPPQSPVAWSNFISGTNP
                     GGHGIFDFIARDAPTLKPFLSTSRISPPDRTLRLGKFSIPVSGGEAEQLRKGPAVWDI
                     IAQAGIDSTAIRAPVNFPPVQSGARTISGLATPDIHGSYGIFSFFTDSPEYRDGPVSG
                     GQIQKTYASAGRYPCVLKGPANTFRRDGAGVDIPFEVQTDAGRTMAQIVLGEDRIILR
                     PGEWSEWVRIKFPMLPVLASASGICRFYLKRTEPHLELYASPVNIDPSEPALPVSSPE
                     DFSRRLVREVGSFYTQGMPEDTSALSAKVFGDDDYRVQATLVHEETLRLFDHALHTYN
                     RGFLYFYFSSLDLNSHAFWRTLDAEHPLYDPEVVKRHGDFLPWLYESIDSAVGRAAGL
                     VDEHTTLYVISDHGFTSFRRQFNLNSWLMDNGYARARSRFDRGHASFFGDTDWSGTKA
                     YGLGINSLYLNLRGREPEGTVGPGAEAEALKDELIARLKAERDPATGQPPVTNVFRPE
                     EIYSGPFVSLAPDLIVCYNMNYRASWDTVLGKYPREVFLDNLDPWSGDHCMDAQFLPG
                     VFLCSRPSGLRDPALTDLAPSILSSCGIPVPREMTGRDLFA"
     gene            2515..3306
                     /locus_tag="PKK36_10865"
     CDS             2515..3306
                     /locus_tag="PKK36_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95093.1"
                     /translation="MFLICGFNRVVSAVCAAVIGPLIGVSGWLALGAVSLLTALIALL
                     VFKYGSSQSRIRRAKDIALARILEIRIYKDDLSGIFGAFGRILLATARYLSLCLFPLL
                     GMMIPIGLMLIHMAAWFEWRPLRNGDTVLVTAAMDGESLPVLESSSGIRIETEAFVVP
                     DQPATWRISVVDDAEPQWIAVTAGGERFVKQAHAGEAGPVYPFLDAFFPGKLANPVEP
                     PLPPSGAVHRLEVQHPKSGRHWIAGWLVLSMAAGLILKYPLRVEI"
     gene            3319..3513
                     /locus_tag="PKK36_10870"
     CDS             3319..3513
                     /locus_tag="PKK36_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95094.1"
                     /translation="MMFKKKIALDPELYAKLSKLSEAQGCASVEEFALHILEDAVKGL
                     GDDASEEEVKKRLRGLGYLE"
     gene            3510..4091
                     /locus_tag="PKK36_10875"
     CDS             3510..4091
                     /locus_tag="PKK36_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNR95095.1"
                     /translation="MTGASARTVVVVSGLPRSGTSLMMQMLQAGGLPLLCDGQRASDE
                     DNPQGYFEFEKVKTLKSDSSWMAEAAGHAVKIVTTHLYDLPAGYDYKIIFMERDLREI
                     LLSQKKMLRRRGASDEGPPDEAMSGHFRRHVGRLKEWLYEQPHIAVMFCRYRDIIEDP
                     SPLCSQIAAFVGRPLNVPAMLGAVDEKLYRNRA"
     gene            4088..>4674
                     /locus_tag="PKK36_10880"
     CDS             4088..>4674
                     /locus_tag="PKK36_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR95096.1"
                     /translation="MKKFAGLTLMLILIAAGAWLVLRPAPISLLIITLDTTRADHLGC
                     YGKSDAATPNLDRLAREGALFEYAYSGVPLTLPSHTTMMTGLRPPEHGLHINGENAVN
                     PEIVTLAEILDGRKYRTGAFIASFVLDSRFGLAQGFQEYNDDVGSGDPSEEGLRAYRN
                     GRDVTDAAIPWLQSAGGKPFFCWVHYFDPHKPYHE"
BASE COUNT          857 a         1319 c         1472 g         1026 t
ORIGIN      
        1 ggtggactgg tcaaagacac aggcctattc cttcgggctg agcggaattt acctcaaccg
       61 caggggtcgc gagtcgcagg ggattgtgga cgatgccggg gtccggcggc tgaaggagga
      121 tatcgcagcc cgattgaagc tggaagacgc cgtgctgcag gtctacgatg cggatgccgt
      181 ttatcggggc ccctatgtca agaacgggcc tgatctgatt gtcggtttcc gagagggctg
      241 gcgcgcttca tgggaatccg cggtgggcac ggtccggacg gaggtcgtga cgcagaacac
      301 aaaggtttgg cagggcgacc actgcgtgga tcagtcgctg gtccccggcg tattcttctg
      361 cagccggcgc ctggctgacg atcggccgct gtctattgcg gatgttgcgc cgacggtcct
      421 ggaggccctg ggggtgcccg ttccggccta tatggatgga tgcgccgtca ccttgaaatc
      481 ggggcggcag tgaattagtg taatcagatg agcagcaggc agaacaaagg tctatatacg
      541 cgccgcgatt ttctgaaaac agccggcgcg gcagcgcttg cgatgggcgt cgcgggctgt
      601 cctgctgtgg tgcggccctc caagggcagg aaccggatca ttgttctggc cgtggacgga
      661 atggacccgc gcctgatgct ggactttatc cgtgccgggg ggatgaagaa ctgcaaagcc
      721 ctgatggagc agggctgctt ttatccgctc cgctcgagcg accctccgca gagcccggtg
      781 gcctggtcca atttcatatc gggaaccaat cccggcggcc acggcatttt tgatttcatc
      841 gcgcgcgatg ctcccactct gaaacctttt ctttcgacct cgcggatttc cccgcccgac
      901 cgtactttgc gccttgggaa attcagcata cccgtctccg gcggcgaggc ggagcagctg
      961 cgcaaggggc ctgccgtctg ggatattatt gcacaggccg gcattgattc gaccgccatc
     1021 cgcgccccgg tcaacttccc gcccgttcag tccggcgcgc gcacaatttc aggtctggcc
     1081 acgcccgata ttcacgggag ctacggcatt ttctcctttt tcaccgacag tccggaatac
     1141 cgggacgggc cggtgtccgg cggacagatt cagaaaacct atgcgtccgc cggcaggtac
     1201 ccgtgcgtgt tgaagggccc cgccaacacc ttccgccggg acggggcggg ggtggatatt
     1261 ccctttgaag ttcagaccga cgctggccgg accatggcgc agattgtgct gggtgaagac
     1321 cgcatcattc ttcgtccggg cgagtggagc gaatgggtcc gcataaaatt cccgatgctg
     1381 cccgtgctgg cttcggcatc aggcatctgc cggttttatc tgaagcgcac agagccgcat
     1441 cttgaactgt atgcctcgcc cgtcaacatt gacccttcgg agccggcgct cccggtttct
     1501 tcgccggagg atttttcgcg caggctcgtt cgggaggtcg gctcgttcta tacgcagggc
     1561 atgcccgagg acacctcggc cctgtccgca aaggtgttcg gcgatgatga ctaccgtgtt
     1621 caggccaccc tggtgcatga ggaaaccctg cggcttttcg atcacgcgct tcacacgtac
     1681 aaccgcggtt tcctgtattt ctatttttcg tcgctggacc tcaattcaca tgctttctgg
     1741 cgcacactgg acgcggagca tccgctgtac gacccggagg ttgtaaaacg gcacggggat
     1801 ttcctcccgt ggctctatga gagcatagac tccgccgtgg gccgggctgc gggtctggtg
     1861 gacgaacata cgactcttta tgtgatctct gatcacggat tcacttcgtt ccgccggcaa
     1921 ttcaatctga actcgtggct gatggacaac ggctatgcgc gcgcccgcag ccgctttgac
     1981 cgggggcatg cgtctttctt cggggatacg gactggtccg gaaccaaggc ctacgggctg
     2041 ggaatcaaca gcctgtattt gaatctgcgc ggccgcgagc cggaggggac cgtgggtccc
     2101 ggtgctgagg ctgaagcctt gaaggatgaa ctgattgccc ggctgaaagc ggagcgcgat
     2161 ccggccaccg gccagcctcc ggtcaccaat gtgttccggc cggaagaaat ctacagcgga
     2221 ccctttgtaa gcctcgcccc ggacctgatt gtttgctaca acatgaatta tcgggcgtcg
     2281 tgggacaccg tgctcgggaa gtatccgcgc gaagtgtttc tcgacaatct ggatccctgg
     2341 agcggagatc actgcatgga tgcgcagttc cttcccgggg tgttcctgtg cagccgtccg
     2401 tccgggctgc gcgatccggc gctgacggat ctggcgcctt ctattttatc gagttgcggc
     2461 attccggttc cgcgtgaaat gaccggaagg gatctgttcg cataggcggg aatcatgttt
     2521 ctgatctgcg gttttaatag agtcgtcagt gcggtctgtg ccgcggtcat tggcccgctg
     2581 atcggggtgt ccggctggct tgctctcgga gctgtctcgc tgctgacggc actcattgcc
     2641 ctgctcgtgt ttaaatacgg atcgagccag agcagaatca ggcgggcaaa ggatatcgcc
     2701 ctggcgcgca ttctcgaaat caggatatac aaggacgacc tgtccggcat cttcggggcc
     2761 ttcgggagaa ttctgcttgc gaccgcccgc tatctttcgc tctgcctgtt tccgctgctc
     2821 ggcatgatga ttccgatcgg cctgatgctg attcacatgg ctgcctggtt tgaatggcgc
     2881 cctctgcgga atggcgacac tgttcttgtg acggcggcca tggacggaga atccctccct
     2941 gtgctggagt cttcatccgg aatccggatc gaaacggaag cctttgttgt gccggaccag
     3001 ccggccacat ggcgcatttc cgtcgtggat gatgcagagc cgcagtggat agcggtgacg
     3061 gccggagggg agcggtttgt caagcaggcg catgcgggcg aggccgggcc ggtttatccg
     3121 tttcttgatg cattttttcc cggaaaactt gcaaaccctg tcgaaccgcc gctgccgccg
     3181 tcgggcgcgg ttcatcgtct ggaagtgcag catccgaaaa gcgggcggca ctggattgcg
     3241 ggctggctcg tgctgtccat ggcggcgggt ctcattttga aatatcctct gcgcgtggaa
     3301 atctgataag gtgaattgat gatgtttaag aaaaaaatag cactggatcc ggagctgtac
     3361 gcgaaactgt caaaactttc cgaagcgcag ggatgcgcgt cggtggagga atttgcgctg
     3421 cacattcttg aggatgccgt gaagggcctt ggggatgatg cttctgagga agaagtcaaa
     3481 aaacgtctcc ggggtctggg atatctcgaa tgaccggagc ctccgccaga acagtggtgg
     3541 tggtgtccgg gcttccgcgt tccggcactt cgctgatgat gcagatgctt caggccggcg
     3601 gcctgccgct gctttgcgac gggcagaggg cctcggatga ggataatcca caggggtatt
     3661 tcgaattcga gaaggtgaaa accctgaaaa gcgattcctc gtggatggca gaagccgctg
     3721 gccatgccgt caagattgtc accacgcatc tttatgatct tcccgctgga tatgattaca
     3781 aaatcatatt catggagcgc gacctgcgtg aaattctgct ctcccagaaa aaaatgcttc
     3841 gccgccgggg cgcgtctgat gaagggccgc ctgacgaagc gatgtccggc catttccggc
     3901 gtcatgtcgg tcggctgaag gaatggctgt atgagcagcc gcacatcgcg gtgatgtttt
     3961 gcaggtaccg ggacatcatc gaggatccgt cgccgctgtg cagtcaaatt gccgcattcg
     4021 tcggccgtcc tttgaatgtt ccggccatgc ttggcgcggt ggatgaaaaa ctttacagga
     4081 acagggcatg aaaaaattcg cggggctgac actgatgctg attctgatcg cggccggggc
     4141 ctggcttgtt ctgcgcccgg cgccgatcag cctgctgatc atcacgctgg acactacgcg
     4201 ggccgaccac cttggatgct acgggaagag cgatgccgcc acccccaatc tggaccgcct
     4261 tgcccgcgag ggcgcgctgt tcgaatacgc gtattcgggc gttccgctga cgcttccatc
     4321 gcacaccacc atgatgaccg gtttgcgccc gccggagcac ggcctgcaca ttaacgggga
     4381 aaacgcggtc aatccggaga tcgtgacgct ggcggaaatc ctggacggga ggaaatatcg
     4441 caccggcgcg tttatcgcct cgtttgtgct cgattcgcgc tttggccttg cgcagggctt
     4501 tcaggaatac aacgatgacg tcggaagcgg cgatccgtcg gaggagggcc tgcgggccta
     4561 tcgaaacggg cgggacgtca ctgatgccgc aatcccgtgg ctgcagtccg ccggcgggaa
     4621 gccttttttc tgctgggtgc attactttga tccccacaaa ccctatcatg agca
//
LOCUS       DAOQWR010000171         4661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18746_length_4661_cov_4.479592, whole genome shotgun sequence.
ACCESSION   DAOQWR010000171 DAOQWR010000000
VERSION     DAOQWR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18746_length_4661_cov_4.479592"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKK36_10885"
     CDS             complement(<1..523)
                     /locus_tag="PKK36_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HNR95097.1"
                     /translation="MHIEKTSDPKTLQLPAGFRAAGISAGIKKSGVKDMALIVSDVEA
                     VMAGTFTTNQVCAAPVKLCRKHLEGRKGRAIIVNSGVANACTGTEGMKAAQDMAAQTA
                     SLLNVPETQVFVCSTGTIGKQLPMDVIGSGIQKIVPAVTVEGVHDAVEAIMTTDTRPK
                     HCTLRIEVDGKPVT"
     gene            complement(596..994)
                     /gene="rpsI"
                     /locus_tag="PKK36_10890"
     CDS             complement(596..994)
                     /gene="rpsI"
                     /locus_tag="PKK36_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNR95098.1"
                     /translation="MVQKVVEYAATGRRKEAVARVNMAPGVGKVSVNGKNFETYFPIE
                     SVRNFIQQPLALTDNLNRFDIAVSVKGGGSMGQAGAVRHAIARALLQVDEALKPALKA
                     ERCLTRDARMKERKKTGQPGARKRFQFSKR"
     gene            complement(1018..1449)
                     /gene="rplM"
                     /locus_tag="PKK36_10895"
     CDS             complement(1018..1449)
                     /gene="rplM"
                     /locus_tag="PKK36_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNR95099.1"
                     /translation="MTKSYVAKKEEVQPQWYIVDAAGKSAGRLAVKLADMLRGKGKPT
                     FTPHVDCGDFVVVINAEKVKLTGAKDTQKIYKKFTGYVGGLKQHSAATVREKDPTRII
                     TQAVRGMLPKNKLSAQVIKKLKVYAGAEHPHGAQNPQPVEL"
     gene            complement(1531..1758)
                     /locus_tag="PKK36_10900"
     CDS             complement(1531..1758)
                     /locus_tag="PKK36_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95100.1"
                     /translation="MTKWYSLFRWGGYLCGAAGLALVLISRRMSPPSAALQAAGGFLL
                     LAMFLCFVITQVLYILYRLKRKKGADRPRDA"
     gene            complement(1768..2388)
                     /locus_tag="PKK36_10905"
     CDS             complement(1768..2388)
                     /locus_tag="PKK36_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNR95101.1"
                     /translation="MYHGERFNSISHVAGAIAALAGLVLLVVLAALKGDPYKIVSFSI
                     YGTTLFLLYLFSSLYHSIRHPSAKAILRKLDHHSISLLIAGSYTPFTLVTLRGGWGWS
                     LFGAVWGLALLGAIVEYLPVRGGRILPVIIYLLMGWMIVIALVPLLKALPRPGFWGLA
                     AGGMFYTVGVGFYAFGRRIRHFHGIWHLFVLAGSACHYFTILLYVL"
     gene            complement(2523..>3103)
                     /locus_tag="PKK36_10910"
     CDS             complement(2523..>3103)
                     /locus_tag="PKK36_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95102.1"
                     /translation="LPWGSLGASGIDDLEWLTVAGAIGSSSTNGKDRYLSAAFVAERS
                     LGARHRAGNFGYSFLTLQPKLVFLAAGDADADGLPNQWEHENFGTSLGPAASDDDDGD
                     QFSNWGEYVAGTEPTNPLSYFIVSPSSPQAVNQNQFVLEWPSAEGRTYELHRSTNDLA
                     SFDLLAANLETGIYTDIVTGIDRAAYRVGVDF"
     assembly_gap    3104..3203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3204..>4661)
                     /locus_tag="PKK36_10915"
     CDS             complement(<3204..>4661)
                     /locus_tag="PKK36_10915"
                     /inference="COORDINATES: protein motif:HMM:NF028070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNR95103.1"
                     /translation="DVVFSGHSHNYQRTVPIRGINYLVAGGGGGTLYYSTFTPGFHEY
                     ATTCLFFVSCHVTGTQMQVRGIRTDGLIFDQTVYTNAGRAVRVEPAFPVRGETVKISY
                     NAANGPLFYSSPVWLYLGTDAFGSAIISTAMTWNAGSGRWEHEFTVPSACTSRLAFAF
                     HDAAVTNWDNNYEYNWQALLERATCDPKTPAAGSNVTIRYEADMGPLRLLGQVFLRAD
                     FGGGASVYTMTNTSGAVWEYTLPVPSEAALMEIFFTAGFSWDNNEGMGWEYEVAGGPG
                     PAEVPPLMAQGTPVISTNPAPQNSAGDNFDFIMSGGAVGSPSAWGGFGDFGSLYFNYD
                     ASNLYVGGIGTDLGGSNNVLMLFLGMNTLTDNATNLWHKSSLPQALDYLHNVGFAEPM
                     DVAIVYGDEYGDDPHYTNFTYGGYDFGQGIFYIGTNSSSFVVMPGARLSQFDGTGTAA
                     CLSDDDDGERRTDRWEASLPWGSLGASGIDDLEWLT"
BASE COUNT          942 a         1424 c         1290 g          905 t
ORIGIN      
        1 cagtgacggg tttgccgtcc acctcaatgc gcagggtgca gtgcttgggc cgcgtgtcgg
       61 tggtcatgat cgcctccacg gcatcgtgaa ccccctcaac cgtcacggcc ggaacaattt
      121 tctgaatgcc tgaccctatg acatccatgg gaagctgttt gccgatggtt cccgtggaac
      181 agacaaaaac ctgcgtttcc ggaacattca gaagcgacgc ggtctgcgcg gccatatcct
      241 gtgccgcctt catgccctcc gtcccggtgc aggcgttggc cacgccggag ttgacgatga
      301 tggcccgccc cttccggccc tccagatgct tccggcagag tttcacgggc gcggcgcaca
      361 cctgattggt cgtgaaagtt ccggccatca cggcttcgac atctgaaaca atcagggcca
      421 tatccttcac tccggacttc ttgatgccgg cggaaatgcc cgccgcacgg aagcccgccg
      481 ggagctgcag cgttttcgga tctgatgttt tttctatatg cataatgaaa ttcctcaaaa
      541 aaatcggccc cgcatgaaaa acatccgggg ccgatcagta aggcttgtac gcgttttaac
      601 gtttcgagaa ctggaagcgt ttgcgcgccc cgggctgccc cgtcttcttt cgctctttca
      661 tgcgcgcatc gcgggtgaga cagcgctccg ccttgagggc gggcttgaga gcctcgtcca
      721 cctgaagcag cgcccgcgca attgcatggc gcaccgcgcc ggcctggccc atgctcccgc
      781 cgcccttgac gctcacggcg atgtcaaagc ggttcaggtt gtcggtcagg gccagcggct
      841 gctggatgaa attgcggacg gattcaatcg ggaaatacgt ctcgaaattc ttgccgttca
      901 cggacacctt gcccacgccc ggggccatgt tcactctggc gacggcctct ttgcgccggc
      961 cggtcgctgc atattcaaca actttctgta ccacgactgt tccctctcgt ttgcctgtta
     1021 aagttcgacg ggctgcgggt tctgggcccc gtgcggatgt tcagcccccg catacacctt
     1081 gagtttcttg atcacctggg cgctgagctt gttcttgggc agcatgcccc gaacggcctg
     1141 cgtgatgatg cgggtcggat ccttctcgcg gaccgtggcg gcgctgtgct gtttcagacc
     1201 gcccacataa cccgtgaatt tcttgtatat cttctgggtg tccttcgccc cggtcagctt
     1261 caccttctcg gcattgatca ccaccacaaa gtcaccgcag tccacatgcg gagtgaaggt
     1321 gggtttgcct ttcccccgca gcatatccgc cagcttgacc gcaaggcggc cggccgattt
     1381 tccggcggcg tccacgatat accactgggg ctggacttct tccttctttg caacatatga
     1441 tttcgtcatc tcaatttcct taatcgccgt aaaaaaacag ggcggaacat aacggaggcc
     1501 cgtaccggtg tcaatcccca tcgccggatt ttatgcgtca cgcggccggt ctgcgccttt
     1561 tttccttttc aggcggtaca atatatacag gacctgtgta ataacaaagc acaaaaacat
     1621 cgccagcaga agaaaacctc cggccgcctg cagggccgcg gagggcggcg acatccgccg
     1681 cgagatcagc accagcgcca gcccggccgc gccgcagaga tatccgcccc accggaacag
     1741 actgtaccat ttcgtcattt gggcctccta cagcacatac agcagaatcg tgaaatagtg
     1801 gcaggcgctt cccgccagca caaacagatg ccagatgccg tggaagtggc ggattctcct
     1861 tccgaaggca taaaatccca cgcccacggt gtaaaacatg ccgccggcgg ccagccccca
     1921 gaaacccgga cgcggaagcg ccttcagcag cggcaccagg gcaatcacga tcatccagcc
     1981 catcagcaga taaatgatca ccggcagtat ccgcccgccg cggaccggca gatactccac
     2041 aatcgccccg agcagcgcca ggccccagac cgctccgaac agcgaccagc cccacccgcc
     2101 gcgaagcgtg accagcgtga acggggtgta gcttcccgca atgaggagcg aaatcgaatg
     2161 atgatccagc ttccgcagaa tcgccttggc cgacggatgg cgtatgctgt gatagaggga
     2221 cgaaaaaaga tacagcagaa acagcgtcgt cccgtaaata ctgaagctga cgatcttgta
     2281 cggatcgccc ttcagcgccg ccaggaccac cagcaggacc aggcctgcaa gcgccgcaat
     2341 ggcgcccgcc acatgactga tgctgttgaa tcgttctccg tggtacatag cgccgcgaat
     2401 ttacgcccgg acccgctgca tggcaatgac attcgcagtt tttccaatcc ctggaagttt
     2461 ttcagcaaaa agttccaacc attggaagtt tttttaacaa aagttccaat cattggaact
     2521 tttcagaagt ccacgccgac gcggtaggcg gcgcgatcga ttcccgtgac gatgtcggta
     2581 tatatgccgg tctcaagatt cgcagccagc aggtcaaacg aggccagatc atttgtggag
     2641 cggtgcagct catacgtgcg gccttccgcc gacggccatt ccagaacgaa ctggttttgg
     2701 ttaaccgcct gcggggagga aggcgataca atgaaatatg aaagagggtt ggtcggttcc
     2761 gttcccgcca catactcacc ccagttgctg aactggtcgc cgtcgtcatc atccgatgcc
     2821 gcagggccca gcgaggttcc gaagttctcg tgctcccact ggttcggaag gccgtcggcg
     2881 tcggcgtcgc cggccgcaag aaaaacgagt ttcggctgga gtgtcagaaa actgtaaccg
     2941 aaatttccgg cgcggtgccg ggcgcccagc gagcgctcgg ccacaaatgc cgcagaaaga
     3001 tagcggtcct ttccattggt gctggaagac ccgatggctc cggccacggt cagccattcc
     3061 agatcatcaa tgccggacgc ccccagcgaa ccccacggca ggcnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnngtcagcc attccagatc atcaatgccg gacgccccca
     3241 gcgaacccca cggcaggctg gcctcccagc ggtcggtgcg gcgttcgccg tcgtcgtcat
     3301 cactcaagca ggctgcggtt ccggttccgt cgaactgcga caggcgcgcg ccgggcatca
     3361 cgacaaagct tgaggagttt gtgccgatgt aaaaaatgcc ctggccgaaa tcatatccgc
     3421 cgtacgtgaa atttgtgtag tgcggatcgt ccccgtactc gtcgccgtaa acaatcgcca
     3481 catccatcgg ttccgcaaat ccaacgttgt gcagatagtc cagcgcctgc gggagcgagg
     3541 atttgtgcca gagattcgtg gcgttgtccg tcagcgtgtt catcccgagg aacagcatca
     3601 gcacgttgtt gctcccgccc agatcggtgc cgatgccgcc gacgtaaaga ttggacgcgt
     3661 cataattgaa gtacaggctg ccgaaatctc cgaacccgcc ccatgcggac ggcgacccga
     3721 ccgcgccgcc ggacatgatg aaatcgaaat tgtcaccggc tgagttctgc ggtgcgggat
     3781 tcgtggaaat cacaggcgtt ccctgagcca tcagcggagg gacctctgcc gggcccggac
     3841 cgccggcgac ttcatactcc cagcccatcc cctcgttgtt gtcccatgaa aagcccgctg
     3901 tgaagaagat ttccatgagg gcggcttcgg acgggaccgg gagggtatac tcccagaccg
     3961 ctccggatgt gttcgtcatg gtataaactg atgcgccccc gccgaagtcg gcgcgcagaa
     4021 aaacctgacc cagcaggcgc agcgggccca tgtccgcttc ataacgaatg gtcacattcg
     4081 atcccgctgc gggcgttttc ggatcgcaag ttgcccgctc aagcagcgcc tgccagttat
     4141 actcgtagtt gttgtcccag ttggtgactg cggcatcgtg gaacgcaaac gcgagccggc
     4201 tggtgcaggc ggaaggcacc gtgaattcat gttcccagcg gccgctgccc gcgttccagg
     4261 tcatcgctgt gctgatgatc gcgctgccga aggcgtccgt gccgagatac agccagacgg
     4321 gggatgaata aaacagcggg ccgttcgcgg cattgtagga tatcttcacg gtttcgccgc
     4381 ggaccgggaa cgcgggctcc acgcgcacgg cgcggccggc gttggtataa accgtctgat
     4441 caaatatcag accgtccgtg cggattcccc gcacctgcat ctgagttccg gtcacatggc
     4501 acgagacaaa gaatagacag gtggtcgcat attcgtgaaa acctggcgtg aaggtggaat
     4561 aatagagcgt gccgcccccg ccgcccgcga ccaaatagtt gatcccgcgg atcggcaccg
     4621 tgcgctgata gttgtggctg tggccgctga acaccacgtc c
//
LOCUS       DAOQWR010000172         4646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_18833_length_4646_cov_4.896319, whole genome shotgun sequence.
ACCESSION   DAOQWR010000172 DAOQWR010000000
VERSION     DAOQWR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18833_length_4646_cov_4.896319"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PKK36_10920"
     CDS             <1..800
                     /locus_tag="PKK36_10920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR95104.1"
                     /translation="NMVALLAAFVPNKTPILMLDCLWEHPRGLLHRLLKQMQFRLQAR
                     TVRRFIVWAVHEIRDFSETFGLPAEMFQFIPFHHSLEGFQYEVSEGDYIFSGGDTDRD
                     YRCLLEAVRGLNVKLRIATRWTGWNEGADVPAGVEAGGTSPEDFRQLMAGAKMVVLPM
                     RRGQLRSAGQQTFLNAMAMGKPVVVADDKGARDYIDSGNDGIVVPSGDVEAMRSAIKS
                     LLDDPEYAHQMGARAREACEKYSTTVCMQRVLASAEEIAGHGGGGKT"
     gene            797..1924
                     /locus_tag="PKK36_10925"
     CDS             797..1924
                     /locus_tag="PKK36_10925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNR95105.1"
                     /translation="MKSLEWKLRAALDGLCLDEKLVRINEYVRLLFTRRRRCGYVGCL
                     GYANLGDRALYEAIQLIFRHHMTFASHRTDMLARLVSAARRGPAFDLVCLGGGTLMGQ
                     TSVYRLESALDAGLPVFSFGTGCHPMQFGLECDPEYGRKFKRQIGILQRLQLVSVRDE
                     ASKRTLLEQGLRAEIDVIGDPVLAFALDSRPYAGGRRIGVNVGNDYGAQMWGRWENIV
                     SALRPVLLALLDDGWEITLFPLSADEIPGTVRLARELGPRIKVFRHYLNRAAYIAACS
                     EQDMFIGVKLHSVILAHCALTPAVMLAYQPKCTQYMESVNMQACTLQTNHLSETSILG
                     ALDEARSAAADIRARLTERFSFYAARRATFVKKVCEMLLDD"
     gene            2018..3115
                     /locus_tag="PKK36_10930"
     CDS             2018..3115
                     /locus_tag="PKK36_10930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95106.1"
                     /translation="MKILILMETEAFSGPAKNLLETLKLLRDEVHFEVATFLRGSAES
                     SAFIEAVRSAGLPVYVIRERFRYDPSAIRHLFRIVQKTAPDIIQIHNTKSRLYVTLLR
                     RRLERLGVREIHYFHGETWVDRKQHFYNRLDRLLFRYAPNVVVVAEYQRELLVGWGVS
                     KDRVAVIHNGIRLAPVDTAHRGEKNYLLTVGRFSKEKGHQILLAAACLLRRREVAGFC
                     LHMVGDGPERQSLERYAAEKGLADIVAFEGYQSDPSDYYKRAGLFILPSLTEGFPNVL
                     LEAASHGVPVISFDVGGIPEIFRNGEEAVLLSDHNPEALADAIQDYLQNPEKHRIMAE
                     KARLRVMREFSLEKKAARLLEYYKEILPQRR"
     gene            3339..3566
                     /locus_tag="PKK36_10935"
     CDS             3339..3566
                     /locus_tag="PKK36_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95107.1"
                     /translation="MTLLKEREIEEREMRARARRARIVAHRALGFRDAENWDLSFWQS
                     KTPQERLSALVDIREDVKKVEEARRNYEQHR"
     gene            3550..4026
                     /locus_tag="PKK36_10940"
     CDS             3550..4026
                     /locus_tag="PKK36_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95108.1"
                     /translation="MNSIADFEDILALFEEHKVHYLIVGGLAFIFHAKPRYTKDMDLW
                     VENSEQNIVRANQALAEYGSPVLLDRRAPGQIVQIGVEPNRIDILVDMGQLSFATAWE
                     KRIESTYGGSRACWIDIESLLTIKSSIDHPRHQEDARVLRQILDQRHKTEPGSGTH"
     gene            4079..>4646
                     /locus_tag="PKK36_10945"
     CDS             4079..>4646
                     /locus_tag="PKK36_10945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR95109.1"
                     /translation="MNLWLKKHLYHLAVPFLVRRARRNNLCFGVSYHRFESVPGADPK
                     PGMAVTPELFRKHIEILRELGSFISIDDAVSEKRPDGISFVLSFDDGYRDNLTILLPI
                     LEELNVPCCLYVTSGLVSGELTALDHDKRAGFCPPVLNPADLKALSAHPLMTIGSHTQ
                     NHVRLSTLDAGALEAELKTSRDWFQKKAE"
BASE COUNT         1023 a         1177 c         1344 g         1102 t
ORIGIN      
        1 gaaacatggt ggctttgctg gctgcttttg ttccgaacaa aacacccata ttgatgctcg
       61 actgcctgtg ggaacacccc cggggcctgc tgcaccggct gctgaagcaa atgcagttcc
      121 gtctgcaggc gcgaacggtt cgccggttta ttgtctgggc cgttcatgag attcgggatt
      181 tttctgaaac attcggcctg cctgccgaaa tgtttcagtt cattccgttt catcacagtc
      241 ttgaaggttt tcaatatgag gtatcggagg gcgattacat attcagcggc ggcgatacag
      301 accgggatta ccgatgcctg ctggaggcgg tccgcggcct gaatgtgaag cttcgcattg
      361 caacgcgatg gacgggatgg aatgagggtg ctgatgttcc cgcgggcgtc gaggcgggcg
      421 gaaccagccc tgaggatttt cggcagctca tggcaggcgc caaaatggtg gttctgccca
      481 tgaggcgtgg acaactccgc tctgccgggc agcagacttt tctgaatgcc atggccatgg
      541 ggaagccggt ggtggttgct gatgacaaag gagcgcgtga ttatatagac agcggcaatg
      601 acgggattgt cgtcccctcg ggtgatgtgg aggccatgag atcagctatc aagtccctgc
      661 tggatgatcc cgagtacgcc catcagatgg gagcgcgcgc ccgggaggcc tgtgagaaat
      721 attccaccac cgtatgcatg cagcgtgtgc tggcctccgc ggaagaaatc gcgggacacg
      781 gcgggggcgg caaaacgtga aatctcttga atggaaactt cgcgctgcgc tggacgggct
      841 ttgtctggac gagaaacttg ttcggatcaa tgaatacgtc cggcttctgt ttacgcgccg
      901 ccgccggtgt ggctatgttg gatgcctcgg ttacgccaat ctgggtgatc gggcgcttta
      961 cgaggccatt cagctgattt tccggcatca catgaccttt gcttcgcacc ggacggatat
     1021 gttggcccgg ctggtatccg cggcgagaag aggaccggct ttcgacctgg tttgcctggg
     1081 cggaggcaca ctcatgggac agacttcagt gtatcggctc gaatcggcgc tggatgcggg
     1141 actgccggtg ttttctttcg gcaccggatg ccatcccatg cagtttggtc ttgaatgcga
     1201 tcctgaatac ggccggaaat tcaagcggca aatcggcata ctgcagaggc ttcaactggt
     1261 atccgtccgg gacgaagctt caaaacgcac gcttcttgag caggggctca gggcggaaat
     1321 agatgtaatt ggcgatcccg ttctggcatt tgcactggat tccaggccct atgccggcgg
     1381 ccggcgtatt ggggttaatg tgggcaatga ttatggtgcg cagatgtggg ggcgttggga
     1441 aaatatcgtg tctgcccttc gtccggttct gctcgcgctg ctggacgacg gctgggagat
     1501 cacgctgttc cctctgtccg cggatgaaat acccggaacg gtccggcttg cccgtgaact
     1561 ggggccgcgg attaaagtct tccgccatta tctgaatcgg gcggcttata ttgccgcctg
     1621 ttcggagcag gatatgttta ttggagttaa gctccattcg gtgattcttg cgcattgcgc
     1681 cctcactccg gccgtgatgc tcgcatatca gcccaagtgc acgcaataca tggagtccgt
     1741 aaacatgcag gcctgcacgc tgcagacaaa tcatctgtcg gaaacttcca ttctgggcgc
     1801 gctggatgaa gcaaggtctg ccgctgcgga tatccgggcc cggctgaccg aaagattttc
     1861 cttttatgcc gcccgtcgcg ccacgtttgt gaaaaaggtt tgtgaaatgc tgctggatga
     1921 ttaggctgca gccaggaaaa gaaatccgat attccatgat tccggcattc caaagctgca
     1981 acttgcaact tggaaccttt aactttcaac tgaaaccatg aaaatcctga tcctgatgga
     2041 aacagaggcc ttcagcggac cggcgaagaa tctgctggag accctgaaac tgcttcgcga
     2101 tgaagtgcat tttgaagtgg caacctttct tcgcggaagc gcagaaagct cggcattcat
     2161 tgaggccgtc cgcagcgccg ggctgccggt ttatgtgatt cgggagcgct tccggtacga
     2221 tccgtctgcg ataaggcatc tcttccgcat agtgcagaaa acagctcctg acattatcca
     2281 gattcacaac acgaaatccc gcctgtatgt cacgttactc cgtcggcggt tggaacgatt
     2341 gggcgtgcgg gaaattcatt atttccacgg cgaaacctgg gttgaccgca agcagcattt
     2401 ctacaaccgt ctggaccgcc tgttgttccg atatgcgccc aatgtggttg ttgtggctga
     2461 atatcagcgg gaacttctgg ttggctgggg ggtctccaaa gaccgggttg cggtgattca
     2521 taacggtatc cggcttgccc cggtggatac ggctcatagg ggcgagaaaa attatctgct
     2581 gacagttggc cgattttcaa aagaaaaggg acatcagatt cttctggcgg ccgcttgtct
     2641 cttaaggcgg cgtgaagtgg ccggcttctg cctgcatatg gtcggggacg ggcctgaacg
     2701 ccaatcgctg gaaagatacg cggctgaaaa aggccttgcg gatattgttg cctttgaggg
     2761 ttatcagtcc gatccgtcgg actattacaa gcgcgcgggc ctgttcattc tcccgtcgct
     2821 gactgagggc tttcccaatg tcctccttga ggcggcgtcg cacggcgttc ctgtgatcag
     2881 ttttgatgtg ggcggcattc cggagatatt cagaaacggt gaagaggccg tgcttctttc
     2941 ggaccacaat cctgaggcgc tggcggatgc catccaggat tatctgcaaa accccgagaa
     3001 gcaccggatt atggcggaaa aggcccgcct gcgcgtgatg cgggaattca gcctggaaaa
     3061 gaaagcggca cgattgctgg agtactataa agaaattctc ccgcagaggc gctgagacgc
     3121 agagcagtta atatctctgc tttgaaaaat gaacgtgtcc ggatttcgga aacgatacat
     3181 attgcataaa tccttatccc cctctgcgcc tctgcgcgag tgtgattgaa agcccgaaga
     3241 caagagacga gatccctctt gaataattca aggcttgaga cgcggtccag aaatgttaga
     3301 ttgatggcaa gagggagtat gataaggaat cactggttat gacgctgcta aaagaacggg
     3361 aaatcgaaga gcgggaaatg cgcgcgcgcg cgcgccgtgc aagaattgta gcccatcgag
     3421 ctttgggatt tcgggatgcg gaaaactggg atttgtcgtt ctggcaatcg aagacccctc
     3481 aggaacgact gtctgccctc gttgacattc gcgaagatgt gaaaaaagtg gaagaagccc
     3541 ggcggaatta tgaacagcat cgctgatttt gaagatattc ttgcgctttt tgaggagcat
     3601 aaggttcatt atctcattgt gggcgggctg gctttcattt ttcatgcgaa accgcgttat
     3661 acaaaagaca tggatttgtg ggtggaaaac tccgaacaga atattgttcg tgccaatcag
     3721 gctcttgcgg agtacggaag tcccgtgctt ttggatcgcc gtgcccccgg ccaaatcgtg
     3781 caaatcggcg ttgagcccaa tcggattgat attcttgtgg atatgggtca gctttcattc
     3841 gcaactgcgt gggaaaagag gattgaatcc acttatggtg gaagccgggc ctgctggata
     3901 gacatcgagt ctttactaac catcaaaagc tccattgatc accctcgtca ccaggaagat
     3961 gcccgtgttc tgcgccagat tctggatcaa aggcataaaa cggaacctgg aagcggaacc
     4021 cattaagcgg gcctgcaatt ctcttcaccc caaagtctct tgtctctggt ctatctccat
     4081 gaacctttgg cttaaaaaac acctgtatca cctcgccgtc ccgtttctgg tgcggcgcgc
     4141 ccgcagaaac aatctttgtt tcggagtgag ctatcaccgt ttcgagtccg tccccggcgc
     4201 cgaccccaag cccggcatgg ccgtgacgcc ggagcttttc agaaaacata ttgaaattct
     4261 gcgtgaactg gggtctttca tttccattga cgatgccgtt tccgaaaaac gtcccgatgg
     4321 aatcagtttc gttctcagct ttgacgacgg ctatagagac aatctgacca tactgctccc
     4381 gatacttgaa gagctcaacg tcccgtgctg tctctatgtg acatcggggc ttgtgtccgg
     4441 cgagctgacg gcccttgatc atgataaacg ggccggattc tgcccgcccg tcctgaatcc
     4501 ggccgatctc aaagcgcttt ccgctcatcc gctaatgacc atcggaagcc atacgcaaaa
     4561 tcatgtgcgg ctgagcacgc tggatgcggg tgcgcttgag gcagagctca aaaccagccg
     4621 cgactggttt cagaaaaagg cggaac
//
LOCUS       DAOQWR010000173         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_19162_length_4587_cov_4.626214, whole genome shotgun sequence.
ACCESSION   DAOQWR010000173 DAOQWR010000000
VERSION     DAOQWR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19162_length_4587_cov_4.626214"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="hydE"
                     /locus_tag="PKK36_10950"
     CDS             <1..814
                     /gene="hydE"
                     /locus_tag="PKK36_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNR95110.1"
                     /translation="KWAHASRYGSIVLQGGERQDKKHAEFIEDILRVVKRETNGELGI
                     TLSLGEQPRDTFERWFAAGGHRYLLRIETSNPRLYAKLHPADHSHAARLQCLDDLKAI
                     GYQVGTGVMIGLPDQTLDDLASDIEFFRERDIDMIGMGPYIVHSETPLAAEMPDFAEK
                     ARGQLDLALRMIAVTRLYLRDVNIASTTALQALDAQGREMGLQAGANIIMPNITDTKY
                     RDAYQLYENKPCLDENAALCRNCLQRRVESVGETIGFGEWGDSPHFQKRTGS"
     gene            817..2058
                     /gene="hydF"
                     /locus_tag="PKK36_10955"
     CDS             817..2058
                     /gene="hydF"
                     /locus_tag="PKK36_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNR95111.1"
                     /translation="MESTPKGMRVQIGIYGRRNVGKSSVLNALTRQQVSIVSDVAGTT
                     TDPVEKAMEMLPIGPVLFIDTAGIDDIGALGEKRVTKTRKIIERTDIALLVTSAAALP
                     LDSFEQSLLDEFRRKKVPVIVVVNRCDEHLPAQKEIESLQNAGLAVAQTAAVTGEGMD
                     ALRVALINAVPAEMLEPHTIAGDLIPKGEMAMLVVPIDLEAPKGRLILPQVQTVRDIL
                     DHDSYCMVAKESELRAALGRLNRPPALVVTDSQAFRQVSEIVPPEIPLTSFSILFARL
                     KGDLPTFVEGAMAIDRLKPGDRVLVAEACTHHPIGEDIGRVKIPQWLREYVGGDLHFD
                     TVQGADFPEDISRYQLIIHCGSCTFNRRQLLTRIVQCRELNIPMTNYGLAIAFSLGIF
                     ARALEPFPQAREIYLRGPKKA"
     gene            2244..>4587
                     /locus_tag="PKK36_10960"
     CDS             2244..>4587
                     /locus_tag="PKK36_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR95112.1"
                     /translation="MSTADASLTTGLPGLDAVLKGVLAGDNIVWQVDSIDEYQEVAAP
                     YCEAAVRNGRRLIYFRFARHQPLVAAGPGVEVHELDPEEGFESFIEKIHGVIEESGPG
                     AFYVFDCLSRLAVDWCSDQMLANFFMLTCPYLFNLETVAYFSLYRNYHTTHALGPIQN
                     TTQLFLDTYRHKGVLYVRPVKVQHRYSPTMHMLHLRRGEEFLPVTASAVITEILSASK
                     WSGLSSDTSAGFWETSFFQARELIKSGEYRPDIPEKGKDLFSQLVRMIVSRDERMRDL
                     VARHLTLQDILDIRQRMIGTGLIGGKTVGMLLARMIVRKALPRFAERMEEHDSFFIGS
                     DVFYSFLVRNGIWWVRQKQRDPEKYLENAEQARRLIITGEFPEYILRQFDEMLDYFGQ
                     SPFIVRSSSLLEDNYGNSFAGKYDSVFCANQGPRERRMQDFLAAVKAIYASSMSEKAL
                     QYRARRGMLDKDEQMALLVMRVSGASYGQYYFPQFAGVGFSFNPYAWHESIDPQAGVV
                     RLVFGLGTRAVDRADDDYTRVVALNAPDRRPESNFDKMAQYAQRRVDCLDLEGNQLVS
                     RPFHEVVAHAGDIPLDMFTTSDPPGSPGVLTFSRLLSKTPFVADMRELMQALESAYGC
                     PVDIEFAANFTEGDKYKINLLQCRPLQVQGSGTVEVPRVHVAEQDRIIEARGAVVGHS
                     RTGRIERFIYVSPELYGKLPAAERYEAARIIGALNRADAPGPVMLLGPGRWGTSTPSL
                     GIPVSFSEINKISVLCEIVAMHEHLVPDVSLGTHFLNELVE"
BASE COUNT          941 a         1262 c         1408 g          976 t
ORIGIN      
        1 gaaatgggca cacgcaagcc gctatggatc cattgttctg cagggcggcg agcgtcagga
       61 caaaaagcat gcagagttca ttgaagacat cctgcgcgtt gtgaagcgcg agacgaacgg
      121 ggagctggga atcacccttt ccctgggcga gcagccgcgc gacacctttg agcgctggtt
      181 tgccgcgggc ggtcaccgct atcttctccg catcgagact tccaaccccc ggctttatgc
      241 caaacttcat ccggccgacc acagccatgc ggcgaggctg caatgccttg atgatctcaa
      301 ggccattggc tatcaggtcg gcacgggggt gatgatcggc cttccagacc agacgctgga
      361 cgatctggcc tccgacattg aatttttccg ggagcgcgac atcgacatga tcggcatggg
      421 gccgtacatt gtgcacagcg aaaccccgct ggcggccgag atgccggatt ttgcggagaa
      481 ggcccgcggc cagctcgatc tggcgctgcg aatgatcgcg gtgacgcggc tctatttgcg
      541 cgatgtgaac attgcgtcca caacggcgct gcaggcgctg gatgcgcagg gccgcgagat
      601 gggcctgcag gcaggcgcga acatcatcat gcccaacatc acggacacga aataccgcga
      661 tgcctaccag ctttacgaaa acaagccgtg ccttgacgaa aacgccgcgc tgtgccgcaa
      721 ctgtctgcag cgccgggtgg agtcggtggg cgaaacaata ggcttcggcg agtggggcga
      781 ttcaccgcac tttcaaaaaa ggaccggatc atgaaaatgg agagcacgcc caaaggaatg
      841 cgcgtccaga tcggcatcta cggccggcgc aatgtcggca agtcgtcggt cctgaatgcg
      901 ctcacgcggc agcaggtttc cattgtttcc gatgtggcgg ggacgacgac ggatcccgtt
      961 gagaaggcca tggaaatgct cccgatcggc ccggtgctgt ttatagacac ggccggcatt
     1021 gacgatatcg gcgcgctggg cgaaaaaagg gttactaaaa cccgcaaaat catcgagcgc
     1081 acggatatcg ccctgctggt gacatcggcc gccgcgctcc cgctcgattc cttcgagcag
     1141 tcgcttctgg acgagttccg caggaaaaag gtgccggtga ttgtcgtggt gaacaggtgc
     1201 gatgagcatt tgcctgcgca gaaggaaatt gaatcacttc agaatgccgg cctggccgtg
     1261 gcgcagactg ccgcggtgac aggcgaaggg atggatgcgc tgcgcgtggc gctgatcaat
     1321 gccgtgcccg cagagatgct ggaaccgcac acaatcgccg gagatctcat tccaaaagga
     1381 gagatggcga tgctggtggt gccgattgat cttgaggccc cgaagggccg cctgatcctg
     1441 ccgcaggtcc agactgttcg cgacattctt gatcatgaca gctattgcat ggtggcgaag
     1501 gaaagcgaac tgcgcgcggc gctggggcgg ctgaaccgcc cgcctgcgct ggtcgtgacc
     1561 gattctcagg ctttccggca ggtgtccgaa atcgtacctc cggaaattcc gctgacctct
     1621 ttctcgattc tcttcgcgcg gctgaagggc gatctgccga cctttgttga aggggcgatg
     1681 gccattgacc ggctcaagcc cggcgaccgg gtgctggtgg ccgaggcctg cacgcatcat
     1741 ccgatcggcg aggacatcgg ccgcgtgaaa attccgcaat ggctgcggga atatgtcgga
     1801 ggcgacctgc atttcgacac cgttcagggc gcggatttcc ccgaagacat cagccggtac
     1861 cagctgatca tccactgcgg atcctgcacc ttcaaccgca ggcagctcct gacgcgcatc
     1921 gtgcagtgcc gggaactgaa cataccgatg accaactacg gtctcgccat tgcgttctcg
     1981 ctgggcatct ttgcccgcgc cctcgagcct ttcccgcagg cccgcgagat ttatctgcgt
     2041 gggccgaaaa aagcctgaaa aaagggaaaa acggcgaaaa tcgctcatca atgggcaaaa
     2101 acgggaccca gtagggtcta aaagaatcca aagcaaaaaa atctgtcggg ataaatgcca
     2161 ttgcatcccg aatccgtcgg cttgcaaatg aaagcggctg ccggtagcgt tcctgtgact
     2221 gaatacgaaa ggagcgttat gaaatgagta cggcagatgc ttcgctgacc acagggttgc
     2281 ccgggcttga tgcggtgctc aagggcgtgc tggccggaga caacattgtc tggcaggtgg
     2341 attcgattga tgaatatcag gaggtggcgg cgccgtactg cgaggctgct gtccgtaacg
     2401 gacgcaggct gatctatttc cgctttgccc ggcatcagcc gcttgtggcc gcagggcccg
     2461 gagtcgaagt gcatgaactg gatcccgagg agggctttga gagttttatc gaaaagatcc
     2521 atggcgtgat cgaagaatcc ggccctgggg ccttttatgt atttgactgt ctttcccggc
     2581 tggcggtgga ctggtgttcg gaccagatgc tggcgaattt cttcatgctg acgtgtccgt
     2641 atcttttcaa tctggagacg gtggcctatt tttcgctcta tcgcaattat cacaccacgc
     2701 atgcgctggg gcccattcaa aacacgacac agctgttctt ggacacgtat cggcataaag
     2761 gtgtgttata tgtgcgtccc gtgaaggttc agcaccgtta ttcacccacg atgcacatgc
     2821 tgcatctgcg gcgaggggag gagttcctgc ctgtgacggc cagtgccgtg atcacggaaa
     2881 tcctttccgc gtccaaatgg tccggtctgt cttccgacac ttctgccggt ttctgggaaa
     2941 catctttttt ccaggcgcgc gagctgatca agtcgggtga gtatcgcccg gacattccgg
     3001 agaagggcaa ggacctgttt tcgcagctgg tgagaatgat cgtttcccgt gatgagcgca
     3061 tgcgggatct ggtcgcccgg catctgacgc ttcaggacat tcttgatatt cgacagcgca
     3121 tgataggcac gggactgatc ggagggaaga ccgttggaat gctgctggcc cgcatgatcg
     3181 tccgcaaggc gctgccacgg ttcgcggaac ggatggagga gcatgactcc ttcttcattg
     3241 gttcagatgt attctacagt tttcttgtgc gcaacggcat ctggtgggtg cggcagaagc
     3301 agcgtgatcc ggaaaaatat cttgagaacg ctgaacaggc ccgccgcctg atcatcactg
     3361 gtgaattccc cgagtatatt ctccggcagt ttgacgagat gctcgattat ttcgggcagt
     3421 ctccgttcat tgtgcgctcc agttcactgc ttgaagataa ttacggcaat tcctttgccg
     3481 gcaagtatga cagcgtattt tgtgcaaacc aggggccgag agagcgccgc atgcaggatt
     3541 ttctggcggc cgttaaggcc atttacgcga gctcgatgag cgaaaaagct cttcagtacc
     3601 gtgcccgccg cggtatgctg gacaaggatg agcagatggc cctgctggtg atgcgcgttt
     3661 ccggagcctc ttacgggcaa tactacttcc cccagtttgc cggcgtcgga ttttccttca
     3721 acccgtatgc gtggcacgag agcattgatc ctcaagcggg ggttgtgcgg ctggtgtttg
     3781 gtctcggtac gcgtgcggtg gaccgagcag acgatgacta tacccgcgtg gtggcgctga
     3841 atgcgcccga ccgccggcct gaatccaatt tcgacaagat ggcccagtac gcccagcgtc
     3901 gtgtggactg ccttgatctg gaaggcaacc agctggtttc acgtcctttt catgaggtgg
     3961 tggcccatgc cggggatatc ccgctggaca tgttcacaac gtctgacccg cccgggtcgc
     4021 ccggcgttct gacgttcagc cgcctgcttt caaaaactcc ttttgtggcg gacatgcggg
     4081 aactgatgca ggcactggaa tccgcctatg gttgtccggt ggacatagaa tttgccgcta
     4141 atttcacgga gggggacaaa tataaaatca atctgctcca atgccgtccg ctgcaggtgc
     4201 agggttcggg cacggtggag gttccccggg ttcatgtggc tgagcaggac cggatcatcg
     4261 aagcccgcgg cgcggtggtg ggccacagcc ggacaggccg aatcgagcgc ttcatttatg
     4321 tctcacccga actttacggc aaactgccgg cggcagaacg ctatgaggca gcccggatca
     4381 tcggggccct gaaccgcgca gatgcccccg gccccgttat gcttcttggg cccggccggt
     4441 ggggcacgag tacaccgtct ctgggtattc ccgtatcgtt ttcagagatc aacaagatat
     4501 cggtgctgtg cgagattgtg gcgatgcatg agcaccttgt gcctgatgtt tcgctgggca
     4561 cgcatttcct gaacgaactg gttgaga
//
LOCUS       DAOQWR010000174         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_19824_length_4473_cov_3.646446, whole genome shotgun sequence.
ACCESSION   DAOQWR010000174 DAOQWR010000000
VERSION     DAOQWR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19824_length_4473_cov_3.646446"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4441)
                     /locus_tag="PKK36_10965"
     CDS             complement(<1..4441)
                     /locus_tag="PKK36_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNR95113.1"
                     /translation="MKKHSYLGLVCTLILALGVPAIAVDYYINDLSDSNDVYCSAIGD
                     DSNPGTDPALPKLTLTNLLATIDLEPGDTVYIDSGYYTNNVVEITTNDQGSVGSYVTF
                     QGSTNDWGKGTFFVGSNTTSHILFHLIAVNSVRLRNLVLINAYRGIYCQNALNCTFEN
                     IRFTSCNRGFMTANNLNNLSLRSCMFALNGGALYLAGSGTGANIDNCVIYSNSYAIFY
                     GNSASISNSIIVGDGPFSGTFPLRADHNILWTTNIGFGYDSLASVQKDLGTWTRSTVV
                     DPQFADPGAYDFHLKSISERYNPETGFWETDTVHSAAIDFGNPVYSAANEPEPNGGRR
                     NIGLHGNTWQASRSRTNLWLLALSYNDGGSLSAPIDSVYWTGNLTNGETVRIDLSGDG
                     GSTWEVAGTGLLASAGTWLWSNTNLLSSRRARWRIMYEADTNVFDTCNTNFVFKTGGF
                     RYYINDASTNRDIYATTIGSDANLGTTSNAPKASLQSILTEYDLEPGDTVFIDTGTYN
                     LSANPEIKASNGGDSNDYVYIRGSTNGTVFIRNNLGSTYALYLNDADYVDISNMEFRR
                     AGTGIYLLSAYNINLSNVRAVSNATAGIIVNASQNVRMNRLVASLNGTYGILQSGSSS
                     LQAKNCILWRNSLVGLQVGISSSATISNSIVAASGPLAYAYHTRRPNQILGDYNNLYA
                     ESNAVLGYLSGIEKNIDNLSEWISRTGQEKNSLTTDPLFANPQGGDFHLKTETALGRY
                     VAGLGYVTNDAVTSPLIDAGNPAEGAPLEGGRKNIGLYGNTVEASQGRVDPWIYAATL
                     EGGGIVSSTQMIHWVACNTTNGTTVDLEASVNGGDSWFALASGIPATNEIYAWNTTAT
                     NDSPSVLFRVVSPSVTDLFNEENVFSRIRNSGPGNYYVNDADGTDDMYTAAEGATNNW
                     FATSNAPMSSLDRVLSMFNLEPGDRVYVDSGTYTSSANAVVSRLDSGMTTNPVIIQGT
                     THEPVTKSVIQRSDSGTNSHVLHLLGAGNVFVRDLILGGGGSGIRADTAWGIMLERLA
                     IRGAAGPGMDILNSTSVSVQRVISESNAGWGMQSSASSVGVQHSLIWSNTLGAVSRSG
                     GAVTLYNNILGAAGAGAYVYSTETASGFVSDYNNILISDLARMAFYNFDVYPSAITWR
                     DATSNDVRSLSHDPKFNDAAAGDYHLLSPAGRYDPGTGFFVTNVAEAFSVLIDAGNPA
                     HSAAAEQAPNGGRVNIGLYGASSQASKSPETNGWLTVLTLNGGGTLRGTNTLYWLAGG
                     IATGHTYTLQVSLNNGVTWSNIVTGLAAGTDFYTWNSTLWGSSAEARWRVVSDTDAGI
                     FGTNDVAFLLDNGALTFYVNDASTNGDVYTTSPGSVLNSGLYSNKPKASIVDVLQSYG
                     LKAGDRILVDTGIYTNNSDLNINGTIFGVSTNPVIIQGSTNFSSTGTVLDRQGGSYGF
                     YVSDTEYIQIRHFTIQNAATAVQIDGADNCLVVGVKTMGGSTAF"
BASE COUNT         1040 a         1262 c         1155 g         1016 t
ORIGIN      
        1 caaaggccgt gctgcccccc atggtcttaa ctcccaccac gaggcagttg tcagctccgt
       61 caatctgcac agccgtggcc gcattctgaa tggtgaaatg ccgaatctga atatactccg
      121 tgtcactgac atagaatccg taactgccgc cctgccggtc cagcacggtg ccggtcgaac
      181 tgaagtttgt gcttccctga atgatcacgg ggttggtgga tacgccaaat atggttccgt
      241 tgatgtttaa atcactgttg ttcgtataaa ttccggtatc taccagaatg cggtcacccg
      301 ctttgaggcc atagctctgc agaacgtcca caatggaagc cttgggcttg ttggaataca
      361 aaccgctgtt cagcacgctt cccggcgagg tggtatacac atcaccattt gtagaagcat
      421 cgttcacata gaaagtcagc gccccgttat ccagcaggaa cgccacatca ttggtgccga
      481 agatcccggc atccgtgtca ctgaccaccc gccagcgcgc ttccgccgac gaaccccaga
      541 gcgtggaatt ccatgtatag aaatcagttc ccgccgcgag gcccgtcaca atgttcgacc
      601 aggtcacgcc gttgttgagc gaaacctgca gggtgtacgt gtggcccgtg gcaatgcccc
      661 cggccagcca gtaaagggta ttcgtgccgc gaagcgttcc cccgccattc agcgtcagta
      721 cagtcagcca cccgttggtt tccggagatt tgctggcctg tgacgaggcc ccgtacagac
      781 cgatgtttac acggccgccg ttgggcgcct gttctgctgc cgccgaatgc gcaggatttc
      841 ccgcatcaat cagaacagaa aaagcttccg caacattcgt gacaaagaac cccgttcccg
      901 gatcataacg gccggccgga ctcaacagat gataatcccc cgcagccgca tcgttgaatt
      961 ttggatcatg cgaaaggctc cgcacatcgt tggatgtcgc atcccgccag gtgatggcgg
     1021 aaggatacac gtcaaaatta tagaacgcca tccgcgccag gtctgaaatc agaatgttgt
     1081 tgtagtcgga aacgaaaccg gaagccgtct ctgtgctgta cacataggct ccggcgccgg
     1141 cggcccccag gatgttgtta tacagcgtta ccgccccccc ggaacgcgac acggctccca
     1201 gcgtgtttga ccagatcagc gaatgctgaa caccgacgga agaggccgaa gactgcatcc
     1261 cccaaccggc attggactcg gaaatcaccc gctgaacgga tacactggtt gagttcaaga
     1321 tgtccattcc ggggcctgca gcacccctga tggccagccg ctccagcata atgccccacg
     1381 ccgtgtcggc gcggattccg gatccgcctc cacccagtat caggtcccgg acgaatacat
     1441 tcccggcacc gagcagatgc agcacatgag aattcgtgcc ggaatcgctc cgttgaataa
     1501 cagattttgt gaccggctca tgagtcgtcc cctgtataat gaccggattg gtggtcatcc
     1561 cgctgtccag gcgcgaaacc accgcattgg ccgacgaagt ataagtgccg ctgtccacat
     1621 aaacgcggtc ccccggctcc aagttgaaca tactcagcac ccggtccagc gaactcatgg
     1681 gggcatttga agttgcaaac cagttgttcg tcgcgccttc agccgcggtg tacatgtcat
     1741 ccgtgccgtc ggcatcgttc acatagtagt tcccgggccc cgagtttcga atgcgggaaa
     1801 atacgttctc ctcattgaac aaatccgtca ccgaggggga caccacgcgg aaaagcactg
     1861 acggcgagtc gttggtggcc gtcgtgttcc atgcatagat ctcattggtg gcggggatgc
     1921 cgctcgccag ggcaaaccag ctgtcgccgc cattcacgga agcttccaga tcaacagtgg
     1981 tgccgttggt cgtgttgcag gcaacccagt gaatcatctg ggtactggag acgataccac
     2041 cgccctcaag cgtggcggca tatatccacg gatccacccg gccctgactg gcctcgacag
     2101 tattgccata cagaccgatg ttcttgcggc cgccttccag cggagcacct tctgccggat
     2161 tgcctgcgtc aatcagcgga gaggtcacag catcgtttgt cacatacccc agtcccgcga
     2221 cataacgccc cagcgccgtt tcggttttga gatggaaatc tccgccctga ggattggcaa
     2281 aaagcggatc cgtcgtcagg ctgttcttct cctgtccggt gcggctgatc cattcggaaa
     2341 gattgtcaat gttcttttca atgcccgaca aatacccgag cacagcattg ctttccgcat
     2401 aaaggttgtt gtaatccccc agaatctggt taggacgtct tgtatgatat gcataggcca
     2461 gcgggcccga tgctgccaca atgctgttgc taatggtcgc ggaagaactg atcccaacct
     2521 gcagcccgac caggctgttt ctccacagaa tgcaattttt agcctgaaga gatgaagatc
     2581 ccgattgaag gataccgtaa gtcccgttga gtgaggcaac cagcctgttc atccggacat
     2641 tctggcttgc attaacgatt atgccggccg tggcattgga gacggcacgc acattgctca
     2701 aattgatgtt atacgccgac aggagataga tgcccgtgcc cgcccggcgg aactccatgt
     2761 ttgaaatgtc cacataatcc gcatcgttca gatacaaagc ataagtgctt cccaggttgt
     2821 tccgaataaa gaccgttccg ttcgtgctgc cccggatgta cacataatcg ttggaatcgc
     2881 cgccgtttga ggccttaatt tccggattgg cgctcagatt gtaggttcct gtatcaatga
     2941 aaactgtatc accgggctcc aggtcatatt ccgtcaggat cgactgcagc gaggctttcg
     3001 gcgcattgga tgtagttccc aggttggcat cactgccaat cgttgttgca tatatgtcgc
     3061 ggttggttga ggcatcattg atgtagtagc ggaaaccgcc cgtcttgaac acaaagttcg
     3121 tattgcaggt gtcgaatacg ttcgtatccg cctcatacat gatcctccag cgggcacgtc
     3181 gactggacag aagattggtg ttggaccaca gccatgtgcc cgccgaggcc agaagcccgg
     3241 ttcccgcgac ttcccatgtg cttccgccat cacccgaaag gtcaatacgc accgtttccc
     3301 cgttggtcag attccctgtc cagtagactg aatcaatggg agcggaaaga gatccgccat
     3361 cgttgtaaga caaggccaga agccacaggt tggtgcggct cctgctggcc tgccaagtgt
     3421 tgccgtgcag accaatgttc cggcgcccgc cgtttggctc cggttcattg gctgcgctgt
     3481 aaacaggatt tccgaaatca attgcggcgg aatgcacggt gtctgtttcc cagaatcccg
     3541 tttccggatt ataccgctcg gaaatgctct tcagatggaa gtcataagcg cccggatctg
     3601 caaactgtgg atcgactacc gtagaccgcg tccatgtccc aaggtccttt tgaacactgg
     3661 ccagagagtc atatccaaaa cctatattcg ttgtccaaag aatattatgg tctgcccgca
     3721 aagggaaggt ccccgaaaaa ggcccatccc ccacaatgat agagttggaa atcgatgctg
     3781 aattcccgta aaaaatggcg tatgagttgc tgtatataac gcaattgtca atatttgcgc
     3841 cagtaccgga acctgctaga tataacgctc cgccatttaa cgcaaacata catgagcgca
     3901 gactaaggtt gttcagatta ttggcggtca tgaaaccccg gttgcaggag gtgaaccgga
     3961 tgttctcaaa agtacaattc agggcattct ggcaataaat ccccctgtac gcatttataa
     4021 gaacaagatt cctgagcctg acagagttga ctgcaatcag gtgaaacaat atatgactcg
     4081 tggtgttcga tccgacaaaa aaagtgccct tcccccaatc atttgtgctc ccctgaaatg
     4141 tgacataact gccaacggat ccctggtcat ttgtcgtaat ttccacgaca ttgtttgtat
     4201 aataaccgga atcaatgtag accgtgtctc cgggctccag atcaatcgtg gctagaaggt
     4261 tggtgagtgt aagtttgggg agggccggat cggtgcccgg attggaatca tcgccaatgg
     4321 ctgagcagta gacgtcattg ctgtcgctca ggtcgttgat ataataatca acggcaatgg
     4381 caggaacgcc caatgcaagg atgagtgtac aaaccaaccc cagataactg tgttttttca
     4441 tcgcagtatc ctactgtttt gcccctctcg ccg
//
LOCUS       DAOQWR010000175         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_20276_length_4404_cov_3.610715, whole genome shotgun sequence.
ACCESSION   DAOQWR010000175 DAOQWR010000000
VERSION     DAOQWR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20276_length_4404_cov_3.610715"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="PKK36_10970"
     CDS             <1..1563
                     /locus_tag="PKK36_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNR95114.1"
                     /translation="IRSDSDKLLAPLKSNANALSGIFDKVLTFAKGETIDGSVWDGGR
                     YCPTPTIIEAALALYKGHSVADISRSDAGAKNLHQTTDAISEIIRNAKNHSHKAICFV
                     TGVPGAGKTLIGLNIATKHLDEHNDMYSVFLSGNGPLVKVLQEALARDKVLREKEQKR
                     KIKKGIASSEVKMFVQNVHHYRDEYLRDPKPPVDHVALFDEAQRAWNLQKTCDFMRRK
                     KKRPDFQQSEPEFLISCVNRHPDWGVIVCLVGGGQEINTGEAGIGAWLEALQRSYPNW
                     HIHVSDRLLDSEYGAGAVLEGLAGRPHVHYNKDLHLSVSMRSFRAENVSTLVKQVLDL
                     DATDARETLKQIKSKNYPIVITRDLVKAKQWLKTKARGSERYGMVVSSQAERLKPLAI
                     NVRSPMNPIHWFLADKKDVRSSYYLEDVATEFHIQGLELDWACVTWDGDFRYSEKGWQ
                     HREFRGHKWNQIHKPDRQIYQKNAYRVLLTRARQGMVIVVPPGDPEDPTRKSSYYDPT
                     FEYLKNIGFEII"
     gene            complement(1460..1705)
                     /locus_tag="PKK36_10975"
     CDS             complement(1460..1705)
                     /locus_tag="PKK36_10975"
                     /inference="COORDINATES: protein motif:HMM:NF015790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95115.1"
                     /translation="MTDTISKERRSWNMSRIRSKDTRPEIVVRCNYFIPFSQALELYE
                     RSSFRLFRTQCSSDIQTSGRNRKICGSDLRDRLGAQR"
     gene            1967..2248
                     /locus_tag="PKK36_10980"
     CDS             1967..2248
                     /locus_tag="PKK36_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR95116.1"
                     /translation="MLSDRWLTFRELMAYLKIGRSKLYKLLQEGTIPASRIGKSWRID
                     REEVDAWMKTQRAIPTRDDLEGEPPLEPEVLELHSKAGSASLPAEPGTE"
     gene            2255..3490
                     /locus_tag="PKK36_10985"
     CDS             2255..3490
                     /locus_tag="PKK36_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNR95117.1"
                     /translation="MLMEEQTIEKDEEVKNDVPEWIRQDREAIRTVDALAKHVRLTPE
                     ESRRVAKACERFQMKIPPYYLSLMDPENPSCPIRKQAIPDVRELRILPSELDDPIGDA
                     NKDLNNQPLAAMTHRYPDRVLIYPTPLCGNYCRHCFRRRLAGRREYAPTDKQMEAVLK
                     YVAAHPEVHEVILSGGDPLMLSDSRLTDLLARIKSIPHIRTLRIHSRMPVVNPFRITE
                     ELADALRRFQPLWIVTHFNHPVEVTETAKKHLGHLVDRGIPVLNQGVLLKGINDNPET
                     LRELGWKLIEARVKPYYLHHLDRAQGLSHLRVGVRKGLKILRELRGTMPGYAIPSYIL
                     DIPEGYGKVPLQYHYLSTDDRQQLYVETPDGEYVPYADMSDEKVETPDTAPDIEPLKI
                     YPQDSELQEKLDKMVSDHD"
     gene            3483..3929
                     /locus_tag="PKK36_10990"
     CDS             3483..3929
                     /locus_tag="PKK36_10990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNR95118.1"
                     /translation="MTDSGFKIQDPDLTDPAAREEFISDLSRFLGPYDDPREEMVEAL
                     NNALDRGFVFEIVSPGGVRAGAVVISRTPFRLFQPRYHLAYIATAEEFRGKGLGRALL
                     AEAHRRTGKQLALHVSPSNQTAIAFYERCGWKVKYTRMMPAKKPAG"
     gene            4048..>4404
                     /locus_tag="PKK36_10995"
     CDS             4048..>4404
                     /locus_tag="PKK36_10995"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR95119.1"
                     /translation="MSRARVSIKGLEDYADDDKVVGAVRACLEPLGGMGAFVKPGQKV
                     LLKVNIVTPLAPERAATTHPAMVRAVARLALEAGGRVSVGDSPGIGDPAKVLETCGIA
                     AVMRELKLELADFRNTA"
BASE COUNT         1008 a         1151 c         1267 g          978 t
ORIGIN      
        1 attcgttcag acagcgataa acttcttgca ccgcttaaaa gcaatgcaaa tgcactgagc
       61 ggaatttttg acaaggtttt gacttttgct aaaggggaaa ccatcgatgg ttcggtttgg
      121 gatgggggac gatactgtcc gacgcccacg attatcgagg cggctttggc gctgtataaa
      181 gggcattctg ttgcagatat ttcacgaagt gatgccgggg ctaaaaacct ccatcagacg
      241 acggacgcca tttccgaaat cattcgaaat gcgaaaaatc attctcacaa agcaatatgt
      301 tttgtcacgg gtgtgccggg tgccgggaaa accttgattg gtttgaatat agcgaccaag
      361 caccttgatg agcataatga tatgtatagt gttttcttgt cgggaaatgg cccgcttgtg
      421 aaggtgctgc aggaagcttt ggcgcgcgat aaagtgttac gcgaaaaaga gcagaagcga
      481 aaaattaaaa agggcatagc ctcaagcgag gtaaaaatgt tcgttcaaaa tgttcatcac
      541 tatcgagatg aatatttacg agatcctaaa ccacctgttg atcatgtggc cctgtttgat
      601 gaggcgcaac gcgcctggaa tctgcaaaaa acctgcgatt tcatgcggcg taaaaagaag
      661 cgcccagatt tccaacaatc cgagccagag tttctgatat cctgtgtcaa ccggcatcca
      721 gactggggtg tcattgtctg cttggttggt ggcggacagg aaatcaacac tggtgaagcg
      781 ggtattggag cttggttaga agctttgcaa cgctcctatc ccaattggca cattcatgta
      841 tctgatcgtc tgctggacag cgaatatggc gccggtgctg ttttggaggg gttggctggc
      901 cgaccacatg tccattacaa caaagatctc cacctttcgg tttccatgcg ttccttccgc
      961 gcggaaaatg tctctacgct tgtcaagcag gtgcttgatc ttgatgccac cgatgcacgt
     1021 gaaacattga aacagataaa atcgaagaat tatcctatag tcatcactcg tgatttggtt
     1081 aaagccaagc agtggttgaa aacaaaagcc cgtggttctg aaagatatgg aatggttgtt
     1141 tcgtctcagg ctgagcgcct gaaaccactc gcaattaatg tgcgttcgcc tatgaatcca
     1201 attcactggt ttcttgctga taaaaaggat gttcgctctt catattatct agaggacgtg
     1261 gcaacagaat ttcatattca agggctcgag cttgattggg cttgtgtaac atgggacggc
     1321 gacttccgtt attcggaaaa aggttggcag catcgcgaat ttcgcgggca taaatggaat
     1381 cagattcaca aacctgatcg ccaaatatac cagaaaaatg catatcgtgt gcttcttact
     1441 cgcgcgcgtc aggggatggt catcgttgtg cccccaggcg atcccgaaga tccgacccgc
     1501 aaatcttcct attacgaccc gacgtttgaa tatctgaaga acattgggtt cgaaataatc
     1561 taaaagatga cctttcataa agttccaatg cttgggaaaa cgggatgaaa tagttgcagc
     1621 gcaccacgat ttccggccgg gtgtccttgc tcctgatccg gctcatgttc cacgagcggc
     1681 gctcttttga aatcgtgtcg gtcatgattt gaagagtagc gcggcgcggc ggctttgaaa
     1741 aggctgcctt cgggcggaat ctccgcgtcc ctgcgtctct gcgcgaaaat attcctgttt
     1801 ttgtcgattt tttggatttt tggacgtttt taagcgattt ttggcctgtt attcgcgttt
     1861 tttgaccgtt tttacttgat ttttgccgtc tgatatgtat tatttgatgc tgattaagat
     1921 tatttggtga tttttcggtt ccagaatctc taaaacggag tattttatgc tttcagaccg
     1981 ctggctgacg tttcgtgagc tgatggccta tctcaagatc ggccgcagca agctctacaa
     2041 gcttttgcag gagggaacga ttcccgcctc gcgcatcggc aagtcgtggc ggattgaccg
     2101 cgaggaggtg gacgcatgga tgaagaccca gcgggccata ccaacccgcg acgatcttga
     2161 gggcgagccg ccgctggagc cggaggtgct ggaactgcat tcgaaggccg ggagcgcttc
     2221 gctccccgcc gaacccggaa cggagtgatg cttgatgctg atggaagaac agacgattga
     2281 gaaagacgaa gaagtgaaaa acgatgtgcc ggagtggatc cgccaggacc gcgaagccat
     2341 tcggacggtg gacgcgctgg cgaagcatgt ccgactgacg ccggaggagt cccgccgcgt
     2401 ggcgaaggcc tgcgaacgct tccagatgaa gatacccccg tactatttga gtctcatgga
     2461 cccggaaaat ccgtcctgcc cgatccgcaa gcaggccatc cccgatgtgc gcgaactcag
     2521 gattcttccc agcgagctgg acgatcccat cggcgacgcg aacaaggacc tcaacaacca
     2581 gcctctggcg gcgatgacgc accgctatcc cgaccgggtg ctgatctatc cgactccgct
     2641 ctgcggcaac tactgccggc actgcttccg ccgccgcctc gccggccggc gcgagtacgc
     2701 gccgaccgac aagcagatgg aggccgtcct caaatatgtg gcggcgcacc ccgaagtgca
     2761 cgaggtgatc ctttccggcg gcgatccgct gatgctctcc gacagccgtc tgaccgatct
     2821 gctggcacgg atcaaaagta ttccccacat ccggactctg cgcattcatt cgcgcatgcc
     2881 ggtggtgaat ccgttccgga tcaccgaaga gctggcggat gcgctgcgcc ggtttcagcc
     2941 gctctggatc gtgacgcact tcaatcatcc ggtggaggtg accgaaaccg cgaaaaaaca
     3001 cctcggccat ctggtggacc gcggcattcc ggtgctgaat cagggtgtgc ttctgaaggg
     3061 gatcaacgac aatccggaaa cgctgcgcga gctgggctgg aagctgatcg aggcccgcgt
     3121 gaagccgtac tatcttcatc atctggaccg ggcgcagggt ctgtcccacc tgcgtgtggg
     3181 ggtgcggaag ggcctgaaaa ttctgcgcga gctgcggggg acgatgcccg gctacgcgat
     3241 tcccagctac atcctggaca tccctgaggg ctacggcaag gtgccgctcc agtatcacta
     3301 tctgagcacc gacgaccgcc agcagctcta cgtggaaact cctgacgggg aatatgtgcc
     3361 ttacgccgac atgtcggatg aaaaggtgga aactccggac acggcgccgg acattgagcc
     3421 gctgaagatt tatccgcagg acagcgaact gcaggagaag ctcgacaaaa tggtttccga
     3481 ccatgactga ctccggcttc aaaatacagg atcccgatct gactgatccg gccgcccgcg
     3541 aggagttcat cagcgacctg agccggtttc tggggccgta cgatgatccg cgcgaggaaa
     3601 tggtcgaggc actgaacaac gcgctcgacc gcggcttcgt attcgaaatc gtcagccccg
     3661 ggggcgtccg tgccggggcc gttgttatat cccgcacgcc gttccggctt ttccagcccc
     3721 gctatcatct ggcctacatc gcgacggcgg aggaatttcg ggggaaaggc ctcggccggg
     3781 cactgctggc tgaggcccat cgaaggaccg gcaaacagct ggcgcttcac gtcagtccgt
     3841 ccaaccagac cgccatcgcc ttctatgagc ggtgcggatg gaaagtcaaa tacacgcgca
     3901 tgatgcccgc caaaaaaccc gcgggctgag cggccgcccg gaaaagttcc aatccctgga
     3961 agttttgccg aaaaaacttc cagtcattgg aactttttgc gcgaaaactt ccaatcattg
     4021 gaacttttct ggtagagtgc gcgacacatg agcagggcac gggtcagcat taaagggctg
     4081 gaggactacg cggatgatga caaagtcgtc ggtgcggtgc gcgcctgcct ggagccgctc
     4141 ggcgggatgg gggcgtttgt caaacccggc cagaaggttc tgctgaaggt gaatattgtg
     4201 accccgcttg cgccggagcg cgcggcgacg acgcatccgg cgatggtacg ggccgtggcg
     4261 cggcttgcgc tggaggccgg cgggcgggta tcggtcggcg acagccccgg aatcggcgat
     4321 ccggcgaagg tgctggaaac ctgcggcatc gcagcggtca tgcgcgagct gaagcttgag
     4381 cttgccgatt tcagaaacac cgcg
//
LOCUS       DAOQWR010000176         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_21552_length_4203_cov_4.619335, whole genome shotgun sequence.
ACCESSION   DAOQWR010000176 DAOQWR010000000
VERSION     DAOQWR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21552_length_4203_cov_4.619335"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKK36_11000"
     CDS             complement(<1..654)
                     /locus_tag="PKK36_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNR95120.1"
                     /translation="MHMTGFKCVQCEAEQPKNFGRFVCPSCGGNLDVMYDYDAIRAGL
                     DHEYPFPSDRQDIFKYLPLLPLNDVALAPPLRIGATPLYRASRLGASVGLKNLYVKDD
                     GLNPSASFKDRASSVTLTKARELGAKFVAGASTGNAGSSMACMSASVGAPCVIFVPEK
                     APAAKIAQLLIFGAQVIAVRGTYDDAFDLCYRFCEKHHWFNRNTGFNPYTREGKKTCS
                     "
     gene            complement(813..1754)
                     /gene="arcC"
                     /locus_tag="PKK36_11005"
     CDS             complement(813..1754)
                     /gene="arcC"
                     /locus_tag="PKK36_11005"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNR95121.1"
                     /translation="MNKLVVLAVGGNSLIKDKNHQTVPDQYDAANETSKHITDLLAAG
                     YRVVVTHGNGPQVGFILLRSELSKSTLHEVPLDSCGADTQGAIGYHMAQTLMNQLRRK
                     GLKIPVASVVTQVVVDRNDPGFTNPSKPIGVFYSEEEARKAEAEKGWTLKEDSGRGWR
                     RVVASPEPVEIVEEEAIRTLVEKGMAVVAVGGGGIPVIRLENGDLQGTAAVIDKDRAS
                     CLLAKNLKADIFIISTAVPKVAVNFNKPNQQFLDTMTVAEAKKYAAEGQFAPGSMLPK
                     IEAAIDFLEHGGEKVIITMPEMLGDAIQGKAGTHMTR"
     gene            complement(1818..3020)
                     /locus_tag="PKK36_11010"
     CDS             complement(1818..3020)
                     /locus_tag="PKK36_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNR95122.1"
                     /translation="MKNILAEARKYDKDMTAFLRDIIAIPSESCKEGAVIQRIKQEME
                     KVGFDKVEVDKQGNVLGWIGNRKKFVAMDAHIDTVGIGNPDQWKWDPYKGKLEDGKIY
                     GRGAVDQKAGMASMVYAGKLIKEHNLLGDHGLLVTGTVQEEDCDGLCWQYIINEDKIR
                     PACVVITEPTSLEVRRGHRGRMEIGVTTVGKAAHGSAPERGVNAVYKMAKIVNEIEKL
                     NDRLTGEKFLGKGTVVVSYIDCKTPSLCAVPDKCFIHLDRRLALQDTKESAVAEVEDC
                     IKRAGVDAEVTINTYSAPSYTGLVYKTECYFPTWLLKEDSPELKAGISAYKEAIQEEP
                     VIGKWVFSTNGVATMGMHKIPTIGFGPGNEVTAHQVDEFVAIEDLVRAAAWYAAFPAQ
                     YDQAVKGK"
     gene            3206..>4203
                     /gene="queG"
                     /locus_tag="PKK36_11015"
     CDS             3206..>4203
                     /gene="queG"
                     /locus_tag="PKK36_11015"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNR95123.1"
                     /translation="MSIDRQIKEKALETGFDLCGIAPASAAAHAAEFSAWLDKGFQAG
                     MQWMARDPARRSSAGALVEGAQSVIVLGLSYALPEPPPEIWNDPSRGRISRYAWGPDY
                     HDHIQRMLRDLEQFIQKLAPDSRNKIYVDTGPVLERDYALMAGLGFTGRNSMLIHPGL
                     GSMFFLGEIITTLALEPGGLLQMENPCGKCRRCMDLCPTRAFPEPHVLDCRQCIAYHT
                     IESKEVIPPEIAEKTGNWIFGCDECQTVCPWTKKHQPQNSHPFLKFDPETCCPPLLEL
                     AEMNEEAFRAKFGGTPLERLKLPRLLRNTAVALKNWGAPEAVRALEKLSKHPSALV"
BASE COUNT          757 a         1279 c         1289 g          878 t
ORIGIN      
        1 gctgcaggtt ttcttgcctt cgcgggtgta gggattgaag ccggtgttgc ggttgaacca
       61 gtgatgcttc tcgcagaacc tgtagcagag gtcgaacgcg tcgtcatagg ttccgcgcac
      121 ggcgatcacc tgcgcgccga agatcaggag ctgggcgatc tttgcggcgg gcgccttttc
      181 gggcacgaag atcacgcagg gcgcgccgac gctggccgac atgcaggcca tggaactgcc
      241 cgcgttgccg gtgctcgcgc cggcgacgaa ttttgcgccc agctcgcgcg ccttcgtcag
      301 cgtcacggag ctggcgcggt ccttgaagga cgcgctcggg ttcagcccgt cgtccttgac
      361 ataaagattc ttcagcccga ccgaagcgcc gaggcggctg gccctgtaca gaggcgtcgc
      421 gccgatgcgc agcggcggcg cgagagcaac gtcgttgaga ggcagaagcg gcagatattt
      481 gaagatatcc tgacggtcgg acgggaacgg atattcgtga tcgagcccgg cgcgaatggc
      541 gtcgtagtcg tacatcacat cgaggttgcc gccgcacgag ggacacacaa agcggccaaa
      601 gttcttcggc tgttcggcct cgcactgcac gcacttgaat ccggtcatat gcatttttgt
      661 ctccctgcct gatccctgga aacggtctcg ctgagctcga ccctccagca ttttcaaaac
      721 cgttatcccc gcacttcatg gagggcggag cgctgcgacg ccggctggaa gcggtcgcga
      781 cggagcgcga ctctccagca aagccgcggc ggtcagcggg tcatgtgcgt tccggctttg
      841 ccctggatcg catcgcccag catttccggc atggtgatga tcaccttctc gccgccgtgc
      901 tccagaaaat caatcgccgc ctcgatcttg ggcagcatgc ttccgggcgc gaactggcct
      961 tcggcggcgt atttcttcgc ctcggccacg gtcatggtgt ccaaaaactg ctgattgggc
     1021 ttgttgaaat tgacggccac cttcggcacc gcggtggaga tgatgaaaat atccgccttg
     1081 aggtttttcg ccagcaggca ggaggcgcgg tccttgtcaa tcacggcggc cgtgccctgc
     1141 aggtcgccgt tttcaagccg gatgacggga attccgccgc cgccgacggc gaccacggcc
     1201 atgccctttt caacgagcgt cctgatggct tcctcctcga cgatttcgac cggctcgggc
     1261 gaggcgacga cgcggcgcca gccgcggccg gagtcttcct tcagcgtcca gcccttttcg
     1321 gcctcggctt tgcgggcctc ttcctcggaa tagaaaacgc cgatgggctt ggaggggttt
     1381 gtgaagccgg gatcgttccg gtccacgacc acctgggtca cgacggaggc aacgggaatc
     1441 ttcaggcctt tcctgcgaag ctggttcatg agggtctggg ccatgtgata gccgatcgcg
     1501 ccctgcgtgt cggccccgca gctgtcgagc ggcacttcat gaagcgtgct cttggacagt
     1561 tcggaccgga ggagaatgaa accgacctgg gggccgttgc cgtgagtgac caccacacgg
     1621 taaccggcgg cgagcaggtc cgtgatgtgc tttgacgttt cattggcggc atcatactgg
     1681 tccgggaccg tctgatgatt cttgtccttg atcagggaat tgccgcccac cgcgagaaca
     1741 accagcttat tcatactgca cctcggaaat cagatacccg cgcgccgggt tccggcgcgc
     1801 gggcgggttt gttgatatta tttgcccttg acggcctgat catactgggc ggggaacgcg
     1861 gcgtaccacg ccgccgcgcg caccagatcc tcgatggcca caaactcgtc cacctgatgg
     1921 gcggtgacct cgttgccggg gccgaagccg atcgtcggga tcttgtgcat gcccatggtg
     1981 gcgacgccgt tggtggagaa cacccacttg ccgatgaccg gctcttcctg gatggcttcc
     2041 ttgtaggcag aaatgcccgc cttcagttca ggactgtctt ccttgagcag ccatgtcggg
     2101 aagtagcatt cggtcttgta aaccagaccg gtgtagctcg gggcggagta ggtgttgatg
     2161 gtcacttcgg cgtccacgcc ggcgcgcttg atgcagtcct caacttccgc caccgcggac
     2221 tccttcgtgt cctgcagcgc aaggcggcgg tcgagatgga tgaagcactt gtcgggcacc
     2281 gcgcacagcg acggggtctt gcagtcaatg taggacacca cgacagtgcc cttgcccagg
     2341 aacttctcgc cggtgaggcg gtcgttcagc ttctcgatct cgttgacgat cttggccatt
     2401 ttgtagaccg cgttcacgcc gcgctcgggg gcggagccgt gggcggcctt gccgaccgtc
     2461 gtgacgccga tttccatgcg gccgcgatgc ccgcggcgga cttccaggct cgtcggctcg
     2521 gtgatcacga cgcaggcggg gcgaatcttg tcctcgttga tgatgtactg ccagcagagg
     2581 ccgtcgcagt cctcttcctg caccgtgccg gtaacgagaa gaccgtgatc gcccagcagg
     2641 ttgtgctcct tgatgagctt gccggcgtag accatcgagg ccatgcccgc cttctggtcc
     2701 acggcgccgc ggccgtaaat cttgccgtct tcgagcttgc ccttgtaggg atcccacttc
     2761 cactggtcgg gattgccgat gccgacggtg tcaatgtggg catccatggc gacaaacttc
     2821 ttgcggttgc cgatccagcc gaggacattg ccctgcttgt caacttcaac cttgtcgaac
     2881 ccgacctttt ccatttcctg tttgatccgc tggatcacgg cgccttcctt gcagctttcg
     2941 ctggggatgg cgatgatatc gcggagaaaa gccgtcatgt ccttgtcgta ttttctggct
     3001 tcagcaagta tgtttttcat tccacatctt tccttactta tggataattg accgggtctg
     3061 atccggttga ttcactcaaa aacgcctgaa gttatacccc gcccgcgttt ttcttcaagg
     3121 aataagtgcg ccgcacagaa cttccgatgt tcttttcgcg aaaattatct ccctgccggg
     3181 gcggaggccc ctgtatattc gaagcatgtc aattgatcgg caaatcaagg agaaggcgct
     3241 ggaaactggt ttcgacctct gcggcatcgc gccggcctcg gcggcggcgc atgcggccga
     3301 attttcggca tggctcgata agggatttca ggccggcatg cagtggatgg cccgcgatcc
     3361 ggcgcggcgg agcagcgccg gagcgctggt ggagggcgcg cagtcggtca ttgttctcgg
     3421 actgagctat gccctgcccg aaccgccgcc ggaaatctgg aacgatccct cgcgcgggcg
     3481 catctcgcgg tatgcgtggg gtccggacta tcacgatcac attcagcgca tgctcaggga
     3541 tctggagcag ttcattcaaa agcttgcccc ggattcacga aacaagatct atgtggacac
     3601 cggcccggtg ctggaacgcg attatgcgct gatggcgggc ctcggattca caggacgcaa
     3661 ttccatgctg atccatccgg gactggggtc catgtttttc ctcggggaaa tcatcaccac
     3721 gcttgcgctg gagcccggcg ggctgctgca aatggaaaat ccctgcggca aatgccggcg
     3781 gtgcatggac ctctgcccca cccgcgcgtt ccccgagccg cacgtgctgg actgccgcca
     3841 gtgcatcgcc tatcacacca tcgagagcaa ggaagtcatc ccgccggaga tcgcggagaa
     3901 aacggggaac tggatattcg gatgcgatga atgccagacc gtctgcccgt ggacaaaaaa
     3961 acatcaaccc caaaacagcc atccgttcct gaagtttgat cccgaaacct gctgcccgcc
     4021 gctgctcgaa ctggcggaga tgaacgagga agcattccgg gcgaaattcg gcggcacgcc
     4081 gctggagcgg ctgaaacttc cgagactgct gaggaacacg gccgtcgcgc tcaaaaactg
     4141 gggcgctccc gaagccgtcc gcgccctcga aaaactgtca aaacaccctt ccgcgctcgt
     4201 tca
//
LOCUS       DAOQWR010000177         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_21592_length_4196_cov_5.318039, whole genome shotgun sequence.
ACCESSION   DAOQWR010000177 DAOQWR010000000
VERSION     DAOQWR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21592_length_4196_cov_5.318039"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKK36_11020"
     CDS             complement(<1..1635)
                     /locus_tag="PKK36_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNR95124.1"
                     /translation="MTAQNKAEQLVMGANADPGLVAVEPLTGDAHDEMILYSRKGSKI
                     TVTKEPFRPFLWLEKGSLLKAFEGRHEVVKLAGSGPLNHLASFQSWKDMQAALKLLKQ
                     ETGFTPSVSSAPFFCITDPVEQHLMTTGRTLFKDMAFEALGRMQVDIETYVTRGYDFP
                     NAARPGDRIIAIALADQSGWVEVLSGDEAAMLKQFVELVQERDPDVIEGHNIFKFDLP
                     YLEARARRHKIKLKLGRDGGLLGKRPSRFVTADRTINYPKYSIHGRHVADTLFMVQAY
                     DVARRSLDSYGLKDVARHFELAEEDRTYIPGEEIAATFDRDPEKVMAYARDDILETRR
                     LGDLLSPVYFTQAQMLPMTYQNICVRGSAGKIDGLILREYLRQGHAIGKPDSPREFAG
                     GYTDIFFEGIAKNVHHCDVRSLYPSLTLVRELAPASDELGVYLKLLRHLLEFRVEAKE
                     QMQKTEDAGRRRHLDALQGAYKILINSFYGYLGFSQARFSDFDAAEKVAAEGRKILEA
                     MIDWLRENGGTPVEIDTDGIYFVPPEVKSKAEMERFR"
     gene            complement(1652..2596)
                     /locus_tag="PKK36_11025"
     CDS             complement(1652..2596)
                     /locus_tag="PKK36_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR95125.1"
                     /translation="MAKYLVTGGGGFIGCNLVRTLAAQGRDVRILDNFSTGRRENLED
                     LKKDVEIMEGDIRNPADVRAAVRGVCCIFHFAALPSVVRSVEDPLETHDVNVTGTLNL
                     LIAARDAGAKRLVFSSSSSVYGNTPTLPKHEDMIPMPLSPYAAHKLTGEHYCRIFFEL
                     YGLKTFSLRYFNVFGPRQNPKSHYAAVIPLFIDACVRGEPPLIHGDGGQTRDFTYVQN
                     VVDANLACCTAPDEAAGRVFNAACGDRISVNDLASAIAKLMDSKVRPVHDEPRPGDVR
                     DSQADNRRARELLGWTPKVDFEEGIRRTVEWFTSGHAE"
     gene            complement(2608..>4196)
                     /locus_tag="PKK36_11030"
     CDS             complement(2608..>4196)
                     /locus_tag="PKK36_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95126.1"
                     /translation="FNYRLNPYVRASTHLDYRTKRGFGVGQDFIWGQPREERTNPELG
                     DWGGRLLTYYAQDDQPYEDEEEREEQGGLVEEERYRIRLQHRQTYSPRDYLLAEMNYL
                     SDPEILEEFFRNEYRRNVEPENRISLTHRGDHFVSGILINKRLNDFYGAVDRLPELFL
                     DMSRQQIGTSPFYYEGQNSAAFLQRVFAEQEDREDYEAFRIDSDHKVFWPTRHMGFLN
                     VTPRAGYRATWYSETKDTVTNTEWQAVAMEDVVIGGTNGAGSVTNTYTVFTNVEEVVT
                     EEAGADLRSIFEVGFESSFKAFKVMLDPDMRGNGGLRHVAEPYLDYTYIPELDLEPAN
                     LYQFDYIDGLDKRNDVKLGMRNKFQTKWDGKVHDLLDVDLWTYYYLDTEDDEDSVRAA
                     YFDAEFDPVEWFSLAADGQIAVSDTVLNQVNTRAKFTSESAFDSSLAIEYRHIEDRRN
                     QVAAIIDLVPELRWSLGTYVRYDLDNSHMEEQGYFIRRKSDCLGLTFGYEGRGNDWDF
                     WFQIWLTAMPESAIDVSGHY"
BASE COUNT          837 a         1296 c         1187 g          876 t
ORIGIN      
        1 tcggaaccgc tccatttcag ccttcgactt gacctccggc gggacgaaat agatgccgtc
       61 ggtgtcaatc tcgacgggag ttccgccatt ctcccgaagc cagtcaatca tggcttccag
      121 gattttacgg ccttcggcgg ccaccttctc ggccgcgtca aaatcgctga agcgggcctg
      181 gctgaacccc agatagccgt aaaacgaatt aatcaggatt ttgtaggcac cctgcagcgc
      241 atccagatga cgccggcggc cggcatcctc cgtcttctgc atctgctctt tagcctcgac
      301 ccggaattcc agcagatggc gcaggagttt cagatagacg cccagttcgt cagaggccgg
      361 ggccagttcc cgaaccagcg tgagcgaggg atacagcgag cgtacatcgc aatgatgcac
      421 attctttgca atgccctcaa aaaagatgtc tgtatagcct ccggcaaatt cccgcgggct
      481 gtcgggtttc cctatggcat gcccctgccg caggtattca cgcagaatca gtccgtcgat
      541 ctttccggcg ctgccccgga cgcagatgtt ctgataggtc atcggaagca tctgcgcctg
      601 ggtgaaatac accggggaaa gcaggtcccc gagccgccgc gtctccagga tgtcatcacg
      661 ggcataggcc atcaccttct ccgggtccct gtcaaaggtt gcggcaattt cttctccggg
      721 aatataggtc cggtcctcct ccgcaagttc gaaatgccgg gccacatcct tgagtccgta
      781 gctgtccaga gaccggcggg ccacatcata agcctgcacc ataaacagcg tgtccgccac
      841 atggcggccg tgaatggaat atttcggata gttgatggtc cggtcagccg tcacaaagcg
      901 gctgggtctt ttcccgagga ggcctccgtc gcggccaagc ttcagtttga tcttgtgccg
      961 ccgggcacgc gcctccagat acggcaggtc gaacttgaag atattgtgcc cctcgatcac
     1021 gtccgggtcg cgctcctgca ccagttccac aaactgcttc agcatcgcgg cttcgtcgcc
     1081 tgacagcacc tcaacccatc cggactggtc cgccagcgca atggcaatga tgcggtctcc
     1141 cgggcgggcg gcgttgggga aatcgtagcc tctggtgaca taggtttcta tgtccacctg
     1201 catccgtccg agggcctcaa aggccatgtc cttgaaaagt gtccggcccg tggtcatgag
     1261 atgctgctcc accgggtcgg tgatgcagaa aaagggcgcg gaggacacag acggcgtaaa
     1321 cccggtctcc tgcttcagaa gcttcagcgc ggcctgcata tccttccatg actggaaaga
     1381 ggccagatga ttcagcggtc cgctcccggc cagtttaaca acctcatgac ggccctcgaa
     1441 ggctttgagc agactgcctt tttccagcca caggaatgga cggaagggtt ctttcgtcac
     1501 ggtgatttta ctgcccttgc gggaataaag gatcatctcg tcatgggcgt cgccggtcag
     1561 cggttccacc gccacgaggc cgggatcggc attggccccc attacaagct gttcggcttt
     1621 gttttgcgcg gtcatggggc ggtgctcccc gtcactccgc gtgtccggag gtgaaccact
     1681 caacggtccg gcgaatcccc tcctcgaagt caaccttcgg ggtccagccg agcagttcgc
     1741 gggcgcgccg gttgtcggcc tgcgaatcac gcacgtcacc cggccgcggc tcgtcatgaa
     1801 ccgggcggac cttgctgtcc ataagtttcg ctatggctga agccaggtcg ttcactgaaa
     1861 tccggtcccc gcaggcggca ttgaagacgc ggcccgcggc ttcgtcgggc gcagtgcagc
     1921 aggccagatt ggcatccacc acgttctgga catacgtgaa atcgcgcgtc tgtccgccgt
     1981 ctccatgaat cagcggcggc tcgccgcgga cacaggcatc aatgaagagc gggatcaccg
     2041 cggcataatg ggatttcgga ttctgccgcg ggccaaaaac gttgaaatag cgcaggctga
     2101 acgtcttgag cccgtagagt tcaaagaata tccggcagta gtgctcccct gtcagcttgt
     2161 gggcggcata gggactgagc ggcatcggga tcatatcctc atgcttggga agcgtcgggg
     2221 tgtttccgta aaccgacgat gaactggaaa acaccaggcg ctttgctccg gcatcgcggg
     2281 cggcgatcag caggttcagc gtgccggtca cgttcacatc gtgcgtttcc agcgggtcct
     2341 caacggaacg caccacggac ggaagcgcgg caaaatgaaa gatgcagcag acaccccgga
     2401 cggcggccct gacatcagcg gggttccgga tgtcgccttc cataatctca acgtccttct
     2461 tcaggtcttc gagattctca cggcggccgg tggaaaaatt gtccaggatg cggacgtcac
     2521 gcccctgcgc agcaagcgtg cggacaagat tgcacccgat aaacccgccg ccgcctgtga
     2581 ccagatattt cgccatcacc acgcctctta ataatgcccg ctgacgtcaa tggcgctttc
     2641 cggcatggcg gtcagccata tctggaacca gaaatcccag tcgtttccgc ggccctcata
     2701 cccgaaagtc aggcccaggc agtccgactt gcggcggatg aagtacccct gctcctccat
     2761 gtggctgttg tccaggtcat agcgcacata cgttccgagc gaccagcgca gttccggcac
     2821 cagatcaatg atggccgcca cctgattccg ccggtcctct atgtggcggt actcgatcgc
     2881 cagcgaggag tcaaatgcgc tctcggaggt gaatttcgcc cgggtgttga cctgattgag
     2941 aacggtgtcg ctgacggcta tttgtccgtc cgccgcgagg gaaaaccatt caacggggtc
     3001 gaactccgca tcgaaatagg cggcgcgaac gctgtcctca tcatcttcgg tatcaaggta
     3061 gtagtacgtc cagaggtcca catccagcag atcatgaacc ttgccgtccc acttcgtctg
     3121 gaacttgttg cgcatgccga gcttgacgtc attgcgcttg tccagcccgt caatgtaatc
     3181 gaactgataa agattggccg gctccagatc aagttcgggg atgtaggtat agtccaaata
     3241 gggttcggcc acgtggcgca ggccgccgtt gccgcgcatg tcgggatcca gcatcacctt
     3301 gaacgccttg aaggaagatt caaacccgac ctcaaagatg ctgcgcagat ccgcgccggc
     3361 ctcttcggtg actacttcct ccacattcgt gaacacagtg taggtgttgg tcacagagcc
     3421 cgccccgttg gtcccgccga tcaccacatc ctccatcgcg accgcctgcc actccgtgtt
     3481 ggtcacagtg tcctttgtct cggaatacca ggtggcccgg tagcccgcgc gcggagtcac
     3541 attcaggaag cccatgtgcc gcgtgggcca gaagaccttg tggtcggaat caatgcggaa
     3601 agcctcataa tcctcgcgat cctcctgctc tgcaaaaacc ctttgcagaa aggccgcgct
     3661 gttctgtcct tcatagtaga atggagatgt tccgatctgc tggcgggaca tgtccagaaa
     3721 gagttccggc aggcggtcca cggccccgta gaagtcattc aggcgcttgt tgatcagtat
     3781 gcccgaaaca aaatggtctc cccgatgcgt caggctgatg cggttttccg gctccacatt
     3841 ccggcggtat tcattgcgga aaaactcctc aagaatctcc ggatcgctca gatagttcat
     3901 ctcggccagc aggtaatcac ggggggaata cgtctggcgg tgctgcagcc ggatacgata
     3961 gcgctcttcc tccacaaggc cgccctgctc ttccctctcc tcttcatcct cgtacggctg
     4021 atcatcctgc gcgtaatagg tcagcagccg gccgccccag tcgcccagtt cggggttcgt
     4081 ccgctcctcg cggggctgcc cccagatgaa atcctgccca acgccgaacc cccgctttgt
     4141 ccggtagtca agatgcgtgg acgcccgcac ataggggttc aatctgtagt tgaagg
//
LOCUS       DAOQWR010000178         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_21843_length_4161_cov_4.471992, whole genome shotgun sequence.
ACCESSION   DAOQWR010000178 DAOQWR010000000
VERSION     DAOQWR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21843_length_4161_cov_4.471992"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2014
                     /gene="ppdK"
                     /locus_tag="PKK36_11035"
     CDS             <1..2014
                     /gene="ppdK"
                     /locus_tag="PKK36_11035"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNR95127.1"
                     /translation="AVNVQSMVFGNMGDDSGTGVAFTRDGGSGAAKPMGEYLINAQGE
                     DVVAGIRTPKNLDDMPKEGGSIWPKAYKQLHEIMAKLEAHYKYPQDVEFTVERGNLWM
                     LQTRNAKRTGLAGVRWAVEMATGRDVFTGKTLPKLLSPKEALMTLQGNDLEQLLFPIF
                     DIAAEGKAHVIASGLPAGPGAAVGQIVFEAHTAESMLKKNPHEKLILVRHETSPEDVG
                     GMWAAQGVLTSTGGMTSHAAVVARGWGKCCICGASSLGIDYKKKTVTVGKLVLKQGDW
                     ISLNGSTGKIYQHKIPTQSSPVVSAVVQGHKESTKHPIYKMYKQVSDWANEYRTLKVR
                     TNADSPKDAAAARSFGAEGIGLCRTEHMFFEGDRIWAVREFVLAEDRKGREKAISKLL
                     PYQRADFEGIFKAMKDLPVTIRLLDPPLHEFIPQDGPGQKEMARRLKVPVEAVANRVK
                     QLHEMNPMLGHRGCRLSITYPELCVMQARAIIEAACNVEKKNRIKVLPEIMVPLIGTK
                     AEFLFLEKIIRETADAIIRDRKSKVKYLVGTMIEIPRAALTADEVAESAEFFSFGTND
                     LTQMTFGFSRDDIGVFLPDYLKEKILPSDPFQHIDETGVGQLVQMAVKKGRDSRPGLK
                     CGICGEHGGDPASVKFCHKAGLNYVSCSPYRVPIARLAAAQASIKG"
     gene            2164..3000
                     /locus_tag="PKK36_11040"
     CDS             2164..3000
                     /locus_tag="PKK36_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR95128.1"
                     /translation="MPDIDHTVTIYMREIGTTPLLSREEEVKLAKRIKKKDAAARDLM
                     IRANLRLVVKIARDYASYGLPLLDLISEGNIGLMKAVERFDPKKGGKLSTYAAWWIKQ
                     AIKRALANQSKTIRLPAHMVDKIARLRRAEHELTEHLNRAPTNEELAARLDVSAADVE
                     TWKTVALRPASLDAPLSDDGGSISQLVSDDRVQTPYEELNDKQLQTEMEACLEHLDAR
                     ERDILKYRYGLGGVEAETLEQVGERFNITRERVRQIQNSAVKKLRNMMDGSPDEDAEA
                     EA"
     gene            2997..3518
                     /locus_tag="PKK36_11045"
     CDS             2997..3518
                     /locus_tag="PKK36_11045"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNR95129.1"
                     /translation="MSLDAVKKAIRDVPDFPEKGIIFKDITPVLNDPELFRTVVKEFA
                     ARYKDRGIDKIAAVESRGFIFGSALACELGAGLVPVRKKGKLPAETIEASYALEYGTA
                     TLEIHTDAIRKGERVLIIDDLLATGGTAEATASMVKKLGGDIVEIAFLIELAFLKGRD
                     RLAGCQVFAPIVY"
     gene            3542..4030
                     /locus_tag="PKK36_11050"
     CDS             3542..4030
                     /locus_tag="PKK36_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95130.1"
                     /translation="MDWRIPMISLFIGLSCALADGSVPAEQGSIEWFIEAVRRLPPDE
                     PVEGRRPGYNKYQTQKDHWLGWLEAKENTGTYLRKTAPGRDARWVYNHIVEPKMLLWL
                     IRASGVRPELVQAAARAAEGASSLAGKSAAIRRQVPWPEVAAALTAFKSEPDEGLLPS
                     VK"
BASE COUNT          898 a         1214 c         1344 g          705 t
ORIGIN      
        1 cgccgtcaac gtgcaatcga tggtgttcgg caacatgggc gacgattccg gcacgggcgt
       61 ggcgttcacg cgcgacggcg gctccggcgc ggcaaagccg atgggcgaat acctgatcaa
      121 cgcgcagggc gaagacgtcg tggcgggcat ccgcacgccg aagaacctgg acgatatgcc
      181 gaaagagggc gggagcatat ggcccaaggc ctataagcag ctccacgaaa tcatggcgaa
      241 gctggaagcc cactacaaat atccgcagga tgtggaattc acggtcgagc gcggcaacct
      301 ctggatgctg cagacgcgca acgccaagcg caccggcctc gcgggcgtgc gctgggcggt
      361 tgagatggcc accggcaggg atgtcttcac gggcaagacg ctgccgaagc tcctgtcccc
      421 gaaagaggcg ctgatgacgc tgcagggcaa tgaccttgag cagctcctgt tcccgatctt
      481 cgatatcgcg gccgagggca aggcgcacgt gatcgcatcg ggactgccgg cgggcccggg
      541 cgccgcggtc ggacaaattg tgttcgaggc ccacacggcc gaatctatgc tgaagaagaa
      601 tccgcacgag aagctgattc tggtccgcca cgagaccagc cccgaggatg ttggcggcat
      661 gtgggcggcg cagggcgtgc tgacctccac gggcggcatg acctcgcacg cggcggtggt
      721 tgcgcgcggc tggggcaagt gctgcatctg cggcgcctcc tcgctgggga ttgactacaa
      781 gaaaaagacc gtcacggtcg gcaagctggt gctgaagcag ggcgactgga tcagtttgaa
      841 cggctcgacc ggcaagatct accagcacaa gattccgacg cagtccagcc ccgtggtttc
      901 cgcagtggtg caaggacaca aggaatccac gaagcatccg atctacaaga tgtacaagca
      961 ggtctccgac tgggcgaatg agtaccggac gctgaaggtg cgcaccaatg ccgacagtcc
     1021 gaaggacgcg gccgcggcgc gctcgttcgg cgccgaaggc atcggcctct gccgcaccga
     1081 gcacatgttc tttgagggcg accgcatctg ggcggttcgc gagtttgttc tggcggaaga
     1141 ccggaaaggc cgcgaaaaag ccatctccaa gctgctcccg tatcagcggg ccgacttcga
     1201 gggcatcttc aaggccatga aggatcttcc ggtgaccatt cgcctgcttg atccgcccct
     1261 gcatgagttc atcccgcagg acggccccgg ccagaaggaa atggcccgcc gcctgaaggt
     1321 gccggtggag gcggttgcga accgcgtgaa gcagctgcac gagatgaatc cgatgctggg
     1381 tcatcgcgga tgccgcctgt ccatcaccta tccggaactc tgcgtcatgc aggcccgcgc
     1441 catcatcgag gccgcctgca atgtggagaa aaagaacagg atcaaggttc ttccggaaat
     1501 catggttccg ctgatcggaa caaaagcgga attcctcttc ctcgagaaga ttatccgcga
     1561 gacggccgat gcgatcatca gggaccgcaa gtccaaagtg aagtatctgg tgggaacgat
     1621 gattgagatc ccgcgcgccg cgctgacggc ggatgaagtg gccgagagcg ccgagttctt
     1681 cagcttcggg accaatgacc tgacgcagat gaccttcggc ttcagccgcg acgacatcgg
     1741 cgtgtttctg ccggactacc tgaaggagaa gattcttccc agcgatccgt tccagcacat
     1801 tgatgaaacg ggagtcgggc agctcgtgca gatggccgtc aagaagggcc gcgattcgcg
     1861 tcccggactg aagtgcggaa tctgcggcga gcacggcggg gatccggcca gcgtgaagtt
     1921 ctgccacaag gcggggctga actacgtgag ctgctcgccc tatcgtgtgc cgatcgcgcg
     1981 cctcgctgcg gcgcaggcct cgatcaaggg ctgatcgcga caggctgaaa cggcgggcgg
     2041 gaagcagttc ccgcccgctt tttttccgga accggtcggc cgggcaaagc ttcacttgat
     2101 ttcggcgggg cactttgaca agatgcatcc tcaaaacagc agtgaggttc tgggatttga
     2161 gttatgccgg acattgatca tacagtgacg atatacatgc gcgaaatcgg caccactcct
     2221 ctgctcagca gggaggagga ggtcaagctg gccaaacgca tcaagaagaa ggatgcggct
     2281 gcccgcgacc tgatgatccg agccaacctg cggctggtgg tcaagattgc ccgcgactat
     2341 gcctcctacg gccttccgct gctggacctg atttccgaag gcaacatcgg cctgatgaag
     2401 gccgtcgagc gttttgaccc caaaaagggc ggcaagctga gcacctatgc cgcgtggtgg
     2461 atcaagcagg ccatcaagcg ggcgctggcc aatcagagca aaaccatccg cctgccggcg
     2521 cacatggtgg ataaaatcgc gcgcctgcgc cgggcggagc acgagctcac cgagcatctc
     2581 aaccgcgccc cgacgaatga ggagctcgcg gcccggctgg atgtgtcggc ggccgatgtc
     2641 gaaacctgga agacggtcgc cctgcggccc gcctccctgg atgcgcccct gagcgatgac
     2701 ggcggctcca tcagccagct ggtcagtgac gaccgcgtgc agacgcccta cgaggaactc
     2761 aacgacaagc agctccagac tgaaatggag gcgtgccttg agcatctgga tgcccgcgag
     2821 cgggacattc tgaaataccg ctacggactg ggcggcgtgg aggcggagac gctcgagcag
     2881 gtgggcgagc gcttcaatat cacgcgtgaa agggtgcgcc agattcagaa cagcgcggtg
     2941 aagaagcttc gaaacatgat ggatggcagt cccgacgagg atgcggaggc ggaagcatga
     3001 gtctggatgc ggtgaaaaaa gccattcgcg atgttcccga tttccccgaa aagggaatca
     3061 tcttcaagga catcacgccg gtgctgaacg atcccgaact gttcaggacc gtggtgaagg
     3121 aatttgcggc ccggtataaa gaccgcggca ttgataaaat cgcggccgtg gaatcgcgcg
     3181 ggttcatttt cggttcggcc ctggcctgcg aactgggcgc gggactggtg cccgtgcgca
     3241 agaagggcaa gctccccgca gagaccatcg aagcatccta tgcgctggaa tacggcacgg
     3301 ccacgctcga aatccatacc gatgccatcc gcaaaggcga gcgcgtgctg atcattgatg
     3361 atcttctggc caccggcgga acggcggaag ccacggcctc gatggtgaaa aaactgggcg
     3421 gcgacattgt cgagatcgcc ttcctcattg aactggcttt cctgaaaggc cgcgacaggc
     3481 tcgcgggctg tcaggtcttt gctccgattg tctactgatg cgcgcggcgg ccggacggcg
     3541 gatggactgg cgcatcccga tgatctctct tttcattggg ctgtcctgcg cgctggcgga
     3601 cggaagcgtt ccggctgagc agggaagcat tgaatggttc attgaggcgg tgcggcgcct
     3661 tcccccggac gaaccggttg agggccgccg gcccggatac aacaaatacc agactcaaaa
     3721 agatcactgg ctgggctggc tggaggcaaa agagaacacc ggcacctatc ttcgcaaaac
     3781 cgcgccgggg cgcgacgccc gctgggtcta caaccacatc gttgagccca aaatgctctt
     3841 gtggctcatc cgcgcatccg gtgtccgtcc ggagctggtt caggccgctg cgcgtgccgc
     3901 cgaaggggct tcgtccctcg cgggcaaatc ggccgccatt cgccggcagg tgccatggcc
     3961 ggaagtggcc gccgcgctga cggcgttcaa atcggaaccg gacgaaggac tgctgccctc
     4021 tgtaaagtga tcgcggcgtt tttgccggca tgtgaggaaa gcctcaaaaa ccgtgaaaaa
     4081 cggccaaaaa cggccaaaaa acacgaaaaa acgggttctt ctacgatttt catggaaata
     4141 ttggcccaaa cgcgagatag a
//
LOCUS       DAOQWR010000179         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_22916_length_4018_cov_3.557658, whole genome shotgun sequence.
ACCESSION   DAOQWR010000179 DAOQWR010000000
VERSION     DAOQWR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22916_length_4018_cov_3.557658"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKK36_11055"
     CDS             complement(<1..699)
                     /locus_tag="PKK36_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNR95131.1"
                     /translation="MRVFITGGAGFIGCNVARHHLERGDEIVVFDNLSRRGAQINLEW
                     LHEISAGRLRFVQGDVRSAEALAKALPADAERVYHLAGQVAVTTSVEDPRDDFECNAL
                     GTFNLLEAVRARAPGAIVFFSSTNKVYGEMTDAQVVEADTRYAYRNLPRGISEEQPLD
                     FHSPYGCSKGCGDQYVRDYARIYGLRTVVLRQSCIYGIRQFGVEDQGWVAHFCIAARF
                     GRPINIYGDGKQVRD"
     gene            complement(762..1592)
                     /locus_tag="PKK36_11060"
     CDS             complement(762..1592)
                     /locus_tag="PKK36_11060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR95132.1"
                     /translation="MISVITPSFNMLPYLRRCAASVRDQGASQVEHLVMDGGSTDGTA
                     DWLSVQRDLRHVSEKDNGMYDAINKGFDRAHGDIAAYLNCDEQYLPGTLEFVEEYFRQ
                     HPAVDLLFGGALLIRPDGSLISYRKPYRLRPVYILTDHLYVLSCAMFLRRGIIEKGFR
                     FDTSFRANGDTDFVVRLLHAGCRAAVTPRYLSAFTMTGGNLSAGQTAAKEARRLFDRA
                     PVWAQKTRRLLILARRLEKFLSGAYYQRWPLEYAVYAGEPGVRTAFRETKASFRWKSE
                     "
     gene            complement(1597..3063)
                     /locus_tag="PKK36_11065"
     CDS             complement(1597..3063)
                     /locus_tag="PKK36_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95133.1"
                     /translation="MDQKIGLFAWLLAGFAILIGISVYVLKGGLFNYARALALLLPFV
                     FILLINVRSHWHVLILAFVTAESIVIPVYGLNKLTIEMLLLSAVAFTLVLDYIIKKPV
                     REHPFYYADRLVFCLGIIFAVRFLYDRPGFVGLGMETGGLVNSIYYTIAPWFYFAVRA
                     VFARARLTRQQLWAVLFVSAITNVAMLHFAPSLEYTFWGRKVVNAPAWLFCATALALV
                     ISLRPGLKKWFLFYSISFGMLGLGLISNYRSRTLFFAAEILLIAYFAKIFKQAVIVLI
                     IGGVLGTGAMYVAGGGRLPRVVNRAISIFMDVEKTDYTAGGAYGWEDTFRRELYQIAW
                     GRIRENPIVGSGFGLNVQEALQMLSTFRTVTYAELVAHSGNYHNSVLEIAVRVGLPAA
                     VIFSIFYVVYFLRFFKTIESLEGVDKTWAIAITAFWAANTGMMLMNGGPKEYFVSMVI
                     CGIMAGLTARTLKNSTAPAEAPRAVATPPANVGISLSV"
     gene            complement(3073..3738)
                     /locus_tag="PKK36_11070"
     CDS             complement(3073..3738)
                     /locus_tag="PKK36_11070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNR95134.1"
                     /translation="MLISRQTSVRALPWPMIPVLAWQRIAAGLSLLVRSFWYRAFLRG
                     CGCSCGPEFRVYGRIWIRMRERGAIRIGRGVTLISRFGANLVGLTSPVVLDCRRGGRI
                     EMGDYSGCSGAVISSHSSVKIGRHVKIGGNVRIYDHDFHSLDYQVRRGAEDQLQIKTR
                     PVEIGDDVFIGANAIILKGVSIGDRAVVGAGSVVSRNIGPDEIWAGNPARLIRASAGT
                     QSK"
     gene            complement(3732..>4018)
                     /locus_tag="PKK36_11075"
     CDS             complement(3732..>4018)
                     /locus_tag="PKK36_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95135.1"
                     /translation="YIPGIAAAKITILTCLTFVAKGAFVITATMRKNTAMIILHGAGL
                     AAMWLIGILMFEFGQPSLEHVAWLRFWMSLVVSVGLLGYGLYMTREGAAC"
BASE COUNT          972 a         1248 c         1058 g          740 t
ORIGIN      
        1 atcccgcacc tgtttcccgt cgccgtatat attaatcggc cggccgaaac gggccgcgat
       61 gcaaaaatga gccacccagc cctgatcttc gacgccgaac tgacgaatgc cgtaaatgca
      121 ggactgcctc agcacaaccg tgcgcagccc gtagatgcgg gcgtaatccc ttacatactg
      181 gtcgccgcag ccttttgagc agccgtaggg agaatgaaaa tcaagcggct gctcctcgga
      241 aatgccccgg ggcagattcc ggtaggcgta acgcgtgtcc gcctccacca cctgcgcatc
      301 agtcatctcg ccatacacct tgttggttga ggaaaagaac acgattgccc ccggcgcgcg
      361 cgcgcgaaca gcctccagaa gattgaacgt gcccagcgca ttgcattcaa aatcgtcacg
      421 cggatcctca acggatgtgg tcaccgccac ctgtcccgcc agatggtaca cacgctccgc
      481 gtctgccggc agcgcttttg ccagcgcctc tgcgctgcgg acatccccct gaacaaagcg
      541 cagcctgccg gcggatattt catgcagcca ctccagattg atctgcgcac cccggcgcga
      601 caagttatcg aaaacaacaa tctcatcgcc gcgttcgaga tgatgacgcg caacattgca
      661 cccaataaaa ccggctccgc ccgtgatgaa tactcgcata gttactcctg ttaactgttt
      721 gctgacttaa aatactcggc cgctcagaca aagggcggca atcattcgct cttccagcgg
      781 aagctggctt ttgtctcccg aaaggcggtt cggacacctg gctccccggc ataaaccgca
      841 tattccagcg gccagcgctg ataataggcg ccgctcagga atttctccag ccggcgtgcc
      901 aggatcagca gacgccttgt cttctgcgcc cagaccggcg cccggtcgaa aagccgccgg
      961 gcctcttttg ccgccgtctg gccggcactc agattcccgc cggtcatggt aaaggctgac
     1021 agatatcggg gggttacagc cgcgcgacag ccggcatgca gaagccgcac cacaaaatcg
     1081 gtatctccgt tagcccgaaa agacgtgtca aaacggaatc ctttttcgat aatgccgcgg
     1141 cgcagaaaca tcgcgcagct cagcacatat aaatgatcgg tcaggatata aaccggcctg
     1201 agccggtagg gcttgcggta tgaaatcagc gatccgtccg ggcgaatgag cagggcgccg
     1261 ccaaacagca gatccaccgc cgggtgctga cggaaatatt cttccacaaa ttccagtgtg
     1321 cccggaagat actgctcatc acagttcaga taggccgcaa tatcaccgtg cgcgcgatca
     1381 aaccccttgt tgatcgcatc atacatgccg ttgtcttttt cgctgacgtg ccgaaggtcc
     1441 cgctggacgg aaagccagtc cgccgtcccg tccgtcgagc ctccgtccat gaccaaatgc
     1501 tccacctgtg aagcaccctg atcgcgcacc gaggccgcgc agcggcgcaa atagggcagc
     1561 atgttgaagc tgggggttat taccgaaatc atgtgttcag acggataacg aaatgccgac
     1621 attggccggg ggggtcgcca cggcacgcgg cgcctccgcc ggcgcggtgc tgtttttcaa
     1681 ggttcttgcc gtcagcccgg ccatgatgcc gcatatgacc atgctgacga agtactcctt
     1741 gggccccccg ttcatgagca tcatgcccgt attggccgcc cagaacgccg taatggcaat
     1801 ggcccaggtc ttatccacgc cctcgagaga ttcgatggtc ttgaaaaagc gaagaaagta
     1861 caccacataa aagatgctga atatcacggc cgccggaaga ccaacgcgaa cggcaatctc
     1921 cagaacactg ttgtgataat tcccgctgtg ggccaccagt tccgcgtatg tgaccgtgcg
     1981 aaaagtggag agcatctgga gggcctcctg aacattcagg ccaaatccgc tgcccactat
     2041 cggattttcc cgaatgcggc cccaggcaat ctgatacagc tcccgccgga aggtgtcctc
     2101 ccatccatag gctccgcccg cggtgtaatc tgttttctcc acgtccatga aaatggaaat
     2161 cgcgcgattg accacgcgcg gcagacgccc gccgcccgcc acatacatgg cgccggttcc
     2221 gagcactcct ccgatgatga gaactatcac ggcctgtttg aagattttgg caaaataggc
     2281 aatcagcaga atttccgccg caaagaacag cgtgcggctg cgatagtttg aaatcaatcc
     2341 cagtccgagc atgccaaatg atatggaata gaagagaaac catttcttga ggcccggacg
     2401 aagggaaata acaagcgcga gcgcagtggc gcagaacagc caggccgggg cgttcacgac
     2461 cttacggccc cagaaggtgt actccagcga gggcgcgaaa tgaagcatgg ccacgttggt
     2521 tatggccgaa acgaacaaca cggcccacag ctgctggcgg gtcagccggg cccgcgcgaa
     2581 caccgcgcgc accgcaaaat agaaccacgg agctatagtg tagtatatac tgttgacgag
     2641 acccccggtt tccattccca gccccacaaa accaggccgg tcataaagaa agcgcaccgc
     2701 aaaaataata cccaggcaaa acaccagcct gtccgcatag taaaaggggt gttcccggac
     2761 cggctttttg atgatgtagt ccaggaccag cgtgaaggct accgcgctca gaagaagcat
     2821 ttcgatggtc agcttgttca gtccgtaaac cggaatgaca atggattcgg ccgtgacaaa
     2881 tgccaggatc agcacatgcc agtgactgcg gacattgatg agcagtatga atacaaacgg
     2941 aagcagcagg gccagcgcgc gggcataatt gaacagccca cccttcagca catacacgct
     3001 gatgccgatg agaatggcaa agccggcgag aagccacgca aacaatccga ttttctgatc
     3061 catagtaaaa ctctatttcg actgtgtgcc ggcagaagcc ctgatcaggc gggcgggatt
     3121 gcccgcccag atttcatcgg gaccgatatt ccggctgacc accgatcccg cgccaacaac
     3181 cgcccggtcc ccaatggaca cgcccttcag gatgatggca ttagcgccaa taaacacatc
     3241 atctccgatt tcaacgggcc gggtcttgat ttgaagctga tcctccgccc cccggcgaac
     3301 ctgataatca agtgaatgaa aatcgtgatc ataaattcgg acattccccc cgattttcac
     3361 atgccggccg attttaacag aggagtggct ggatatgacc gcgccggagc atccgctata
     3421 gtctcccatt tcgatgcgcc ccccccgacg gcagtccagc accaccggac ttgtcaaacc
     3481 gaccagattg gccccgaacc gggaaatcag ggttacgccc cggccaattc gtatggcccc
     3541 ccgctccctc atccgaatcc agattcgccc atatacccga aactccggac cgcaggagca
     3601 gccgcacccg cgcaggaacg cgcgatacca gaacgaccgc accagcaggc tgaggccggc
     3661 cgcgatccgc tgccatgcca gcaccggaat catgggccac ggaagcgccc ggacagaggt
     3721 ttgtctgctt atcagcatgc ggctccttcc cgtgtcatat acaggccgta gcccagcagt
     3781 ccgacggaca caacaagaga catccagaat ctgagccacg ccacatgttc cagtgagggc
     3841 tgcccgaatt caaacatcag gattccgatc agccacatgg ccgccagacc ggcgccgtgc
     3901 aggataatca ttgctgtatt cttgcgcatg gtggctgtta taacaaatgc gcccttggcc
     3961 acaaacgtca aacaggtcaa aatggttatt tttgcggcgg caatgcccgg gatgtacc
//
LOCUS       DAOQWR010000180         3991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_23117_length_3991_cov_5.508638, whole genome shotgun sequence.
ACCESSION   DAOQWR010000180 DAOQWR010000000
VERSION     DAOQWR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23117_length_3991_cov_5.508638"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PKK36_11080"
     CDS             <1..1552
                     /locus_tag="PKK36_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNR95136.1"
                     /translation="AEWFLGEEPFWMLNADVAAELDPAPLVHLFRAHRPLAVLWLHPE
                     RGPRTVELQGNRITTFTSRRPGSPGTCTFCGLQLLSPAITQYMPPDGFSSIITAYLNG
                     IRAGQSVLGAAVRDSYWADIGSPEAFLEAQREIRDAHRRRRPGRFLYASESHEGRIHP
                     DARLVNTIVMDHAAVGPRAELHDAIVAPGCRINGRASNLLVPAAAVLTPSETEQAGGT
                     EGVCAEMLPGRGSGRTFIRLHRDRDTLMLVRHTYERAENRRYIPHTALLHEAGVNVPE
                     IFFHSSRKAFTLMEDLGTKSLQEVIASGRNAETALRGTLDQVIRFHEDACALARRRHH
                     ALEPPFNRGLYEWEHGLFCGKYLAEHLHLNAPAIRRIRGELGFAAEALLREPRVLLHR
                     DLQSSNILFKRNAPYFIDYQGMRMGPAAYDLASLLCDPYVMLEEDLQMRLLARHRRRA
                     AAGVEEDVFWLAAVQRLCQALGAYARLGALPQGRRFLDHISPASRMLARALRHVPHFP
                     ALRALVSR"
     gene            1620..2309
                     /locus_tag="PKK36_11085"
     CDS             1620..2309
                     /locus_tag="PKK36_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95137.1"
                     /translation="MRKLLVTGLLALPFFAGCIQVEETITLNADGSGTMELSYSVAEE
                     QAAMMESAVSGEEALDAGEDFSFSADAIRQQFKEMEFEGVELLRADSSVQNGRRHISM
                     AVKFNSLSALAETPLLSERKVSLKKTGEGEYTFSQKANTAAISPESGAEMAAFMQSML
                     QGLKVEIRVKAPGPITASNATETRGNEARWVFDIATNPNALSMIQNLDVQLSFKGDDE
                     KLSGFREYGTK"
     gene            2296..3411
                     /locus_tag="PKK36_11090"
     CDS             2296..3411
                     /locus_tag="PKK36_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR95138.1"
                     /translation="MARNKASGKKKTAAAPKNAGASGLFVKYRDELTALARNIPFQDV
                     VIPEFRDRKWEPVSVRQPRTYARPDAGMQAIGRFLIERGMVDLLQPEDTLHLFEEIHW
                     SVHHIRKLSRKRFKNAKEVHQALREAYALISRIEAAEEEIFIANRRLVARCVRPYFWI
                     GQIWLADFLQEGSKALSNAIRKFDFARGTPFYAYAQRAIQNRLRNYFRDHVRTGALGI
                     QPSREMVLIRSAHEDWTALHKTEPPAQTLSNLTEIPVERIEKLLPLVRQWMRVPQQPL
                     SLDERMGESRSDLHQFIEDETLDSAETLAQRSEIWAAVEQLPQRSKEIMHLRFFEGFT
                     LQEAGDKLGLTRARIKQIQDEALKKLRHLLKHGGKLP"
     gene            complement(3418..3966)
                     /gene="rdgB"
                     /locus_tag="PKK36_11095"
     CDS             complement(3418..3966)
                     /gene="rdgB"
                     /locus_tag="PKK36_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNR95139.1"
                     /translation="MFGAHVEIASALDFPHLPDVEEDGDTFEANAVKKAATLARATGL
                     WALADDSGLAVDALGGRPGVFSARYAGEPTDYHANNRKLLKELNGVENRRARFVCVMA
                     LSDPAGNAKTVRGTCEGRIIHETRGAHGFGYDPLFVPDGREQTFAELGDDIKNTISHR
                     ANALKEARKQWAPYLDGKHTGW"
BASE COUNT          773 a         1297 c         1211 g          710 t
ORIGIN      
        1 agccgaatgg tttctcgggg aagagccctt ctggatgctg aacgccgatg tcgccgcgga
       61 gcttgatccc gcgccgctgg tccatctctt ccgcgcacac cgtccccttg cggtgctttg
      121 gcttcatccg gaacgcgggc cgcgcactgt cgaactgcag ggaaaccgga tcacaacctt
      181 caccagccgg cggccgggat ctcccggcac atgcaccttc tgcggcctgc agctgctttc
      241 gcccgccatc acgcaatata tgcctcctga cggcttcagc agcatcatca cagcctacct
      301 gaacgggatc cgcgcgggac agagcgtgct gggcgccgcg gtcagggatt cctactgggc
      361 ggacatcggc agcccggagg cctttctgga ggcgcagcgc gaaattcgcg acgcgcaccg
      421 ccggcgcagg cccggacggt ttctgtatgc ttcggaatcg cacgaaggcc gcattcatcc
      481 cgatgccagg ctggtgaaca ccatcgtcat ggatcacgcg gcggtcggcc cccgggcgga
      541 acttcacgat gcgattgtcg cccccgggtg ccgcatcaac ggccgcgctt cgaatctgct
      601 cgttcccgcg gcggccgtgc tgaccccgtc cgagacggaa caggccggtg gaacagaagg
      661 ggtctgcgcc gagatgcttc cggggcgggg atcgggacgg actttcatcc gcctgcaccg
      721 ggaccgcgac acgctcatgc tggtccgcca cacgtacgag cgggcggaaa acaggagata
      781 catcccccac accgcgctgc tgcacgaagc cggcgtcaac gtcccggaaa tttttttcca
      841 cagcagccgg aaggcgttca cgctgatgga agacctggga acaaaaagcc tgcaggaagt
      901 catcgcttcc ggccggaatg ccgagaccgc cctccgcggg acgctggacc aggtgatccg
      961 atttcacgaa gacgcctgcg cgctggcgcg ccggcggcat catgcgctgg aaccgccctt
     1021 caaccgcggg ctgtatgaat gggagcacgg cctgttctgc gggaaatatc tcgctgaaca
     1081 cctccatttg aacgcgccgg ccattcgccg aatcaggggg gaactggggt tcgcggccga
     1141 agcccttctg cgcgagcccc gtgtgctgct gcatcgcgat cttcagtcga gcaacatcct
     1201 cttcaagcgg aacgcgccgt atttcataga ctaccagggc atgcggatgg gcccggcggc
     1261 ctatgacctg gcctcgctcc tctgcgatcc ctatgtgatg ctcgaggagg acctgcagat
     1321 gcgcctcctc gcccgccacc ggcggcgggc ggcggcaggc gtggaagagg atgttttctg
     1381 gctggccgcc gtacagcgcc tgtgccaggc cctcggcgca tacgcccggc tgggcgcgct
     1441 tccgcagggc cgccgctttc tcgaccacat ctcgccggcc agccgcatgc tcgcgcgcgc
     1501 gctgcggcat gtgccccatt tccccgccct ccgggcgctg gtatcccgtt gacagatcag
     1561 cctctccaat gcacactttg cgagactgtt tgctgaaccg gattatgaaa gactgcacca
     1621 tgagaaaact gcttgttacg ggactgctcg cgctgccttt ttttgcagga tgcatccagg
     1681 tcgaggaaac catcaccctc aacgcggacg ggtcgggaac catggagctt tcatattccg
     1741 tggccgaaga acaggccgcc atgatggaat ccgctgtcag cggggaggag gcgctggacg
     1801 ccggcgagga tttcagtttc agcgcggacg ccatccgtca gcagttcaag gaaatggaat
     1861 ttgagggggt tgaactgctc cgggccgatt cctcggtgca gaatggccgc cgccacatca
     1921 gcatggcggt gaagttcaac agcctgtccg cacttgccga aacgcccctg ctttccgaac
     1981 ggaaggtctc cctgaagaaa accggagagg gcgagtacac cttctcccag aaggcgaaca
     2041 ccgccgccat cagtcctgaa agcggcgcgg agatggccgc cttcatgcaa agcatgcttc
     2101 agggactgaa ggtggaaatc cgcgtgaagg cgccgggtcc catcaccgca tccaatgcca
     2161 ccgaaacccg cggaaacgaa gcccgctggg tgtttgacat cgccaccaat ccgaatgctc
     2221 tttccatgat tcagaatctc gatgtgcagc tcagtttcaa gggggacgac gagaagctgt
     2281 ccggcttccg ggaatatggc acgaaataag gccagcggga aaaagaaaac tgccgccgcg
     2341 ccaaagaacg cgggcgcgtc cgggcttttt gttaaatatc gggacgaact caccgcgctg
     2401 gccaggaaca tcccgtttca ggacgtcgtc atcccggagt tccgggaccg gaaatgggaa
     2461 ccggtgtctg tccggcagcc ccgcacctac gcccggcccg acgccggcat gcaggccatc
     2521 ggccgctttc tgatcgaacg cggcatggtg gacctgcttc agcccgaaga cacactgcat
     2581 ctgttcgagg aaatccactg gagcgttcat cacatacgga agctgtcgcg gaagcggttc
     2641 aagaacgcaa aagaggtcca tcaggcgctt cgcgaagcct atgcgctgat ttcgcgcatc
     2701 gaagccgcgg aggaggaaat cttcattgcc aaccgccggc tggtggcgcg ctgcgtgcgg
     2761 ccctatttct ggatcgggca gatctggctg gcggattttc tgcaggaggg atccaaggcc
     2821 ctttccaacg ccatccgcaa gttcgacttc gcccgcggaa cccccttcta tgcctatgcg
     2881 cagcgcgcca tccagaaccg gctgcgcaac tacttccgcg atcatgtccg caccggcgcc
     2941 ctgggcatcc agcccagccg cgaaatggtg ctcatccgct ccgcccatga ggactggacg
     3001 gccctgcaca aaaccgagcc gcccgcgcag accctcagca acctgacgga aattcccgtg
     3061 gagcgcatcg aaaagctgct gccgctggtc cgtcaatgga tgcgcgtccc ccagcagccg
     3121 ctttcactgg acgagcgcat gggggaaagc cggagcgatc tgcatcagtt catagaggat
     3181 gaaaccctcg acagcgcgga gacgctcgcc cagcgatctg aaatctgggc cgcggtggaa
     3241 cagctgcccc agcgctcaaa agagatcatg cacctgcgct tctttgaggg cttcacactg
     3301 caggaggccg gcgataaact cggtctgacg cgcgcgcgca tcaagcagat tcaggatgag
     3361 gccctgaaaa aactccggca tctgctcaag catgggggaa aactgccctg agggctttca
     3421 ccagcccgtg tgcttcccgt ccagatacgg cgcccactgt ttccgcgctt ccttgagcgc
     3481 gtttgcgcgg tgcgaaatgg tgtttttaat gtcgtctccg agttcggcaa aggtctgctc
     3541 gcggccgtcg ggcacaaaaa gaggatcata gccgaagccg tgcgcgccgc gggtttcatg
     3601 gataatgcgc ccctcgcagg ttccgcgaac cgtctttgcg tttcccgccg gatcagacag
     3661 agccatcacg cagacaaagc gcgcgcggcg gttttcaact ccgttcagct ccttcaggag
     3721 cttgcggttg ttcgcatgat aatcggtggg ttcgccggca taccgcgcgg aaaaaacgcc
     3781 gggccggccg ccgagggcgt ccaccgccag gcccgaatca tcggccagcg cccagaggcc
     3841 cgtggcccgg gcgagcgttg cggccttctt gacggcattg gcttcaaacg tgtcgccatc
     3901 ctcctccaca tcgggcaggt gcggaaaatc cagcgcgctg gcaatctcca catgcgcccc
     3961 gaacagtttc cggaactcat cgagcttgtg c
//
LOCUS       DAOQWR010000181         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_23363_length_3962_cov_4.097517, whole genome shotgun sequence.
ACCESSION   DAOQWR010000181 DAOQWR010000000
VERSION     DAOQWR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23363_length_3962_cov_4.097517"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PKK36_11100"
     CDS             complement(<1..1184)
                     /locus_tag="PKK36_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNR95140.1"
                     /translation="MNSKRAAWVSVSAAALLLCSGCRSTHPPGKLIASSPFPDREELA
                     VAVSEGRGPVELSQAVSYALATHPRIGAMRAQVFRASGNIAEAVTMRSPEINFGFGRE
                     DEDSSGWARETETGSSRRSGSESSSSSEAGSERSASDSWQGGIFDESSSGTRDSATES
                     QGSQSSRRNTRYTSSSYTVDVEDQNEDSYRLGLRYFPPNPWLLSAAGAAARAERWMAQ
                     AELVVEEHELICDTVEAALKIAYGERMLKVREAFAEDCLAIHEEVRRAMEEGSISRSD
                     YLDARLRLTAADADREREAVQLASWRQRFRMLTGVEPGRVTFSEVEEGAICTLQPEDG
                     TSRLEELARELAPQRPDVLAAHWNTLLYEGEWREARAANYPWFNSVEVSYTRWDSDDR
                     RRR"
     gene            complement(1233..2375)
                     /locus_tag="PKK36_11105"
     CDS             complement(1233..2375)
                     /locus_tag="PKK36_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR95141.1"
                     /translation="MKEQINAVKDQHGRRRFVFTWRRIIRNWPVLGWLLAIAAAIWIY
                     GRSARFGNITGTVDAVVEAVAPLDTARLLSIDVTLGERVRAGQALARLDTTVLDADLA
                     EYEVERVETEGSMARDMLQMVRQFETAVKEAEFQLGQVQMEHKSAQGELDQLKKEYSR
                     LLPLIEKRTLTEDAVAEIRPRLAALEEMMKSYPGLIAVADERLRSAVRDRDQLIEWIG
                     AKDGEDIAGAIARQMQNRLKVLEKSRASRERERNNYVLRATQDGVVASIWGDPGEVII
                     AGMPVIRIVQEKSSQVVGYLPEVYVASMQVGQRAWVWRNSSGSRHRVPAKVTAVSPEV
                     NDLPTRLSPFANQIVRGRRVVLELLEENDLVPGESVQIRTSDGRWF"
     gene            complement(2378..3061)
                     /locus_tag="PKK36_11110"
     CDS             complement(2378..3061)
                     /locus_tag="PKK36_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNR95142.1"
                     /translation="MDEFLTAFAPVEYLGAEQIMLSFLLSFILCSAIAFVYTWTFQGF
                     SYSRSFVHTLILSGIIVCMLIMAIGNNLARGLGILGTLAIIRFRTPVRDPRDMVFLFS
                     TLGIGIACGANTFNVAILGTITISAVALILHWSPFASRREYEGLLRFMLPAEFEHEAD
                     VQDVMRECCSSFALIAMREAVQGDMIEYSYHVKLLDPTYQADLVDGLHAVEGLIGANL
                     LMHRTTVEL"
     gene            complement(3084..>3962)
                     /locus_tag="PKK36_11115"
     CDS             complement(3084..>3962)
                     /locus_tag="PKK36_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNR95143.1"
                     /translation="SVCPAGAGRWTRGSSQLKKNVKEKTLARAQVQVERHEAKYIIPP
                     ALVPEVREYIAPFCLPDPNGTGNPPEYTITTIQFDTADFSLHHAKQRESLNRFKLRAR
                     TYGEPGTGKVYLEVKRKFNGVIVKSRASIPFEAWSEALIREDRLDLRFKNRAEEEGFL
                     EFKRLMREVGAEPVLLLRYVRESYFGKIDHYARVTFDRQLCYLPSNGWTDWGTGREWI
                     AMDTGIVQNQQNRYSGVVLELKTLRDAPQWMIDAVKRFDLIRCGNCKYSSAVWAESLF
                     SGLPGVPDYAVELLGF"
BASE COUNT          746 a         1355 c         1120 g          741 t
ORIGIN      
        1 cgccggcggc ggtcatcgct gtcccagcgc gtataggaca cctcgacact gttgaaccac
       61 ggatagttcg cggcgcgcgc ctcgcgccat tcgccttcat acagcagcgt gttccagtgc
      121 gcggcgagca catcgggccg ctgcggcgcg agttcccgag ccagttcctc cagccgcgag
      181 gtgccgtctt ccggctgcag cgtgcagata gcgccctcct ccacctcgct gaatgtcacg
      241 cggccgggtt caacgccggt cagcatccgg aagcgctgcc gccatgaggc gagctggacc
      301 gcttcccgct cccggtctgc gtccgccgcc gtcagccgca gacgggcatc cagatagtcg
      361 ctgcggctga tgctgccctc ctccatggca cgccggacct cctcatggat ggcgaggcaa
      421 tcttccgcga aggcttcccg gaccttgagc atgcgttcac cataggcaat cttgagcgcg
      481 gcctccacgg tgtcgcaaat gagttcgtgt tcctccacca ccagttccgc ctgcgccatc
      541 caccgctccg cgcgcgcggc ggcgccggcg gccgacagga gccacggatt gggcgggaaa
      601 tagcgcagcc ccagacggta gctgtcctca ttctggtcct cgacgtcaac cgtgtacgac
      661 gaactggtat agcgggtgtt cctgcgcgag gactggctcc cctgcgattc agtcgctgaa
      721 tcacgggtgc cggaggaact ttcatcgaat atgccgccct gccatgaatc ggaagcggac
      781 cgctcggacc ccgcctcgga ggaagagctt gattcggaac cggatcttct gctgcttccc
      841 gtttcggttt cacgcgccca gccgcttgaa tcctcgtcct cgcggccaaa cccgaaattg
      901 atttccgggg aacgcatggt cacggcctcc gcgatgtttc cagacgcgcg gaaaacctgc
      961 gccctcatgg cgccaatccg gggatgagtg gccagggcat aggacaccgc ctgagagagc
     1021 tccacgggcc cgcgcccttc cgacaccgcc accgcaagtt cttcccggtc gggaaagggg
     1081 ctcgatgcaa tcagcttccc gggcggatga gttgaacggc atccgctgca aagcagaagc
     1141 gcggccgccg agacactgac ccacgctgcc cttttcgagt tcatcagcct ccgccattcc
     1201 gcccgcaccg cttttgtatc cgcccggcgc actcagaacc accggccgtc agaggtgcgg
     1261 atctgcacac tttcgcccgg aaccagatcg ttctcctcca gaagctccag caccacgcgg
     1321 cgcccgcgca caatctggtt cgcaaaagga ctcagccggg tcggcaaatc gttcacttcc
     1381 ggcgaaacgg cagtcacttt cgcgggaacc cggtgccggc ttccgctgga attgcgccac
     1441 acccacgccc gctggccgac ctgcatgctg gcgacataga cctcgggcag ataacccacc
     1501 acctgcgatg atttctcctg cacgatgcgg atcaccggca tgcctgcaat gatcacctct
     1561 ccgggatcac cccaaataga ggccacgacg ccgtcctgcg tcgcgcgcag cacatagttg
     1621 ttgcgttcac gctcgcggga ggcgcggctt ttctccagaa ccttcaggcg gttctgcatc
     1681 tgccgcgcga tggcccccgc aatatcttcg ccgtccttcg ccccgatcca ctcgatgagc
     1741 tggtcgcggt cacgcacggc ggaccgcagg cgctcatcgg cgacagcgat caggccggga
     1801 taggacttca tcatctcctc cagcgcggcc agccggggac ggatttcagc cacggcatcc
     1861 tcagtcaggg ttctcttttc aatcagcggc agcagccggc tgtattcttt tttgagctgg
     1921 tcaagttcgc cctgcgcgct cttgtgctcc atttgaacct ggcccagctg gaactcggct
     1981 tccttcacgg ccgtctcaaa ctgccgcacc atctgaagca tgtcccgggc catgctgccc
     2041 tcggtttcca cgcgctccac ttcgtattcg gcgaggtccg catcaagaac cgtggtgtcc
     2101 aggcgcgcaa gagcctgtcc ggccctgacg cgctctccca gcgtcacgtc aatcgaaagc
     2161 agccgggctg tatccagcgg ggccacagcc tccaccacgg cgtccaccgt gccggtgatg
     2221 ttgccaaacc tcgcgctccg gccgtagatc cagatggccg ccgcgatggc cagcagccag
     2281 cccagaacgg gccagttccg gatgatccgg cgccatgtga acacaaaccg cctcctgcca
     2341 tgctgatcct ttaccgcgtt gatctgctcc ttcataccta cagctccacc gtggttctgt
     2401 gcatcagcag attcgcgccg atcagtccct cgaccgcgtg caggccgtcc acgagatcgg
     2461 cctgataggt cggatcgaga agcttgacat ggtatgagta ttcaatcatg tccccctgaa
     2521 ccgcctcgcg catcgcaatc agcgcaaagg acgagcagca ttcccgcatc acatcctgca
     2581 cgtcggcctc gtgttcaaat tcggcgggga gcatgaaccg cagcaggccc tcatactcgc
     2641 gccgcgacgc aaaaggcgac cagtgcagaa tcagcgccac cgccgagatg gttatcgtgc
     2701 ccaggatggc cacattgaac gtgttggcgc cgcacgcgat gccgatgccc agcgtggaaa
     2761 acaggaacac catgtcgcgg ggatcccgca cgggggtgcg gaaacgaatg atcgccagtg
     2821 tgccgagaat gcccaggccg cgcgcgaggt tgttgccgat ggccatgatc agcatgcaga
     2881 cgatgatgcc ggaaaggatc agggtgtgca cgaacgatcg cgaataggag aagccctgaa
     2941 aggtccaggt gtagacaaat gcgatcgcgg agcacaggat gaagctcagg aggaacgaca
     3001 gcatgatctg ttcagccccc aggtattcaa ccggcgcaaa cgctgtcaga aactcatcca
     3061 tagctttcct tgtttttcga ccatcagaaa cccagcagct cgaccgcata atccggcacc
     3121 cccggcaggc cgctgaacag ggactccgcc cacaccgccg aagagtactt gcagttgccg
     3181 caccgtatca aatcaaatct tttcaccgca tcaatcatcc actgcggcgc gtcacgcagc
     3241 gtcttcagct ccagcaccac gccggagtac cggttctgct ggttctgcac gatccccgtg
     3301 tccatcgcga tccattcgcg gcccgtgccc cagtcggtcc agccgtttga gggcagatag
     3361 cagagctggc ggtcaaaagt cacccgcgca tagtgatcaa tcttcccgaa atacgattcg
     3421 cgcacgtagc gcagcagcag gaccggctcc gccccgacct cccgcatcag gcgcttgaat
     3481 tcaagaaagc cctcctcttc cgcgcggttc ttgaagcgga gatcaagccg gtcctcacgg
     3541 atcagcgcct cgctccacgc ctcaaaaggg atcgaggcgc gcgacttgac gatcaccccg
     3601 ttgaacttcc gcttcacctc cagatacacc ttgccggttc ccggctcgcc gtaggttcgc
     3661 gcccggagct tgaaccggtt cagggactcc cgctgcttgg cgtgatgaag tgaaaaatcc
     3721 gcggtgtcaa actgaatggt ggtgatggtg tattccggcg gattgcccgt cccgttcggg
     3781 tccgggagac aaaaaggcgc aatgtattct cttacttccg gcacaagggc ggggggaatg
     3841 atatacttcg cctcgtgccg ctcaacctgg acttgcgccc gggccagcgt cttctctttg
     3901 acgttctttt tcaattgact gcttcccctt gtccatcggc cggcccctgc cgggcagacg
     3961 ga
//
LOCUS       DAOQWR010000182         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_23456_length_3951_cov_2.901181, whole genome shotgun sequence.
ACCESSION   DAOQWR010000182 DAOQWR010000000
VERSION     DAOQWR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23456_length_3951_cov_2.901181"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1183
                     /locus_tag="PKK36_11120"
     CDS             131..1183
                     /locus_tag="PKK36_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95144.1"
                     /translation="MNSRTASDNRSLTRADILKALPICYTLAYVSLLPNFYLVFNATN
                     RLSMLWDRALHASILSAIFLLGSLYAVCGLMSYFALHRWRRLLNFFWTAVLLFLLVRT
                     FVSLAARTHILPLGIQSLLNRTEAKLAYYTVFPLVFGIFFPATTVRRISSFLLLVSPA
                     LLFLIFVPMTYTVYDKGNPMGLRELVARAGGDEPAVTNSLPSVFICIFDEWSYDRSLG
                     DPDAADLMPNTLEIMRAAVAFTNAISAGSQTAVSIPRMLFPTNPAVAGKSAGDLTEAV
                     YACRISAADGSIYDMVPADYTTILSGFLLPYDQLLDHRVDYVANYHDGQAFSRTFGSE
                     LRRLLISQVGWLRFLG"
     gene            1195..1716
                     /locus_tag="PKK36_11125"
     CDS             1195..1716
                     /locus_tag="PKK36_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95145.1"
                     /translation="MLTPVGSIVQARKVIEDAMYAARDIPGPLLGVFHICLPHDPFVW
                     DRDGEKAWRLDLVDKHTVSNYHGNVRYLDTVIRDMAEAVISRDEECLLVLTGDHGWRC
                     DPDLATWPNLNAYPYEEIDLNPESPFRRVPLILYTGRVQDAVIIADTFNTKNLHLLIR
                     RYLAGAEIHSGAD"
     gene            2201..3064
                     /locus_tag="PKK36_11130"
     CDS             2201..3064
                     /locus_tag="PKK36_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95146.1"
                     /translation="MLAVNLVLGLGILVHESILIMGFPAIFLARHYALMGDAPLNRRA
                     WTQYLLNMLTPFLLPVGLFMFLFVYQMVFLDKIRLTQMLTAYMKEFPFVAANADTIVP
                     EAFAPDFAAYYRTQSPWFFRRLFSPRYLISMMPTICCMSVCVLRIYRARLNAYSIFLL
                     AAVCFSPLLMHAVAWDVARLWTYPIISSFLCMWILTEVLDAPHTALAGRKTYMLSALA
                     FLMLNIFVRTPLMDDRQERFSGLVRLFLYGPVIIYFLCMTIRMFQRNRQQSKMEPRID
                     ANKHEYKGKKH"
     gene            3128..>3951
                     /locus_tag="PKK36_11135"
     CDS             3128..>3951
                     /locus_tag="PKK36_11135"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR95147.1"
                     /translation="MKIVFWICLIGLFYIYIGYPLLIWVLARLKGRSVRKAPWPGNFS
                     IMIVAHNEAENLPRKIKSILESPAASQIEEILIASDGSTDDTAQVVRDIKCDLVHLVE
                     YGERRGKAAVLNEVLPACKSEIVVLMDARQTVDPQAFDELLSNFSDASVGVVSGELVF
                     QKPGSEFSDPEKGLDAYWRYEKFIRKQESRFRSVPGATGALYAIRKELFEPFPADAIL
                     DDVAIPMSIVRQGFRCIFDEKALVYDRPSSSPEQEAVRKRRTIAGNAQLAVLWPWLL"
BASE COUNT          779 a          981 c         1072 g         1119 t
ORIGIN      
        1 cttgctcaat cgcggcatcg ggccccctac gtgatcgagg aaattatcga gtagtacata
       61 cgcttctgat gctgacggtc tttcttccca tcatgccctc aacatccttt taaaaaagtt
      121 atctatgtcc atgaacagca gaactgcatc tgataacaga agtctgacgc gtgcggatat
      181 tctaaaggct cttccgatct gttatacgct ggcctatgtg tcccttctgc cgaacttcta
      241 tctggtgttt aatgccacaa accggttgtc catgctgtgg gaccgtgcgc tgcatgcttc
      301 tattctttcg gcgattttcc tgctggggtc gctttatgcc gtttgcgggc tgatgagtta
      361 ttttgccctt catcgctggc gcagactgct gaacttcttc tggacggccg ttctgttgtt
      421 tctgctggtc agaacgtttg tttcccttgc ggcccggacg cacatcctgc ctttgggaat
      481 ccaatccctg ctcaaccgca cggaagccaa gctggcttat tacaccgttt tcccgcttgt
      541 ttttggaata ttctttcctg cgacgactgt cagacgcatc agttcattcc tgctgcttgt
      601 ttcaccggca ctcctgttcc tgatttttgt cccaatgaca tatacagttt atgataaagg
      661 aaatccaatg gggctgcggg aacttgtcgc ccgggccggc ggggatgagc ccgccgtgac
      721 gaattctctg cccagcgtat tcatctgcat cttcgacgaa tggtcttacg atcgttcgct
      781 tggcgacccc gatgctgcag atctcatgcc aaacactctg gagattatga gggcggcggt
      841 ggcattcacc aatgccatca gcgcgggcag tcagacggcc gtttctattc ctcgaatgct
      901 ctttcccacg aatccggccg ttgctggaaa atccgcggga gatttgacgg aggcggtcta
      961 tgcctgccgg atatccgctg cggatgggag tatctacgat atggtcccgg ctgattatac
     1021 gactattctt tcaggatttc tgctgcctta tgatcagttg ctcgaccatc gtgtggatta
     1081 tgtcgccaat tatcatgatg gtcaggcctt ttccagaacc tttgggagtg aactgcgccg
     1141 cctgctgatt agtcaggtgg gctggctgcg ctttctgggg tgagtacgcg atacatgctt
     1201 actccggtgg gttcaattgt tcaggcgagg aaggtgattg aggatgccat gtatgccgca
     1261 cgggacatcc ccggcccgct gttgggagtt ttccatatct gccttccgca tgatcccttt
     1321 gtatgggatc gcgacggcga gaaggcctgg cgcctggatt tggttgataa acacacggtg
     1381 tcaaactacc acggcaacgt cagatatctt gacacagtca tcagggatat ggctgaagct
     1441 gtgatatcaa gggacgagga atgcctcctt gtgctgacgg gagatcatgg ctggcgatgc
     1501 gatcctgatc tggcaacctg gccgaatttg aacgcttacc cgtatgagga aattgatttg
     1561 aatccggagt ccccatttcg tcgcgttccc ctgatcctgt ataccggccg cgttcaggat
     1621 gccgtcatca ttgcggacac attcaataca aagaatttgc atctgctgat acggcgttat
     1681 ctggccggag cggaaatcca ttcgggggca gattgaagcg aaagccgcgt gatatgtcgg
     1741 agttcttatg aagaagagga ggaaatcata ttctctcgga acacattcgt tttttctgaa
     1801 aagcggtctt ttgctctggg tgctgttgat gacggtgctt cgcgcagtgc gacgcccgaa
     1861 cgactgggcg gaggctcact ggctggttga ttacagattc ggttttatta aaagggggct
     1921 gacgggcttt cttttttcga aaatctgtgg agatgctgtt gccccggaac tggccattct
     1981 ccttgtttcc agtgcgctgc tgatgctctt tctctttgtg gtatggctgt tcagtgtgcg
     2041 catatggatt cgcacgggtc atcaagagga agttctggtt gttctttgtc tctttgtgtc
     2101 gtccccgtat tttgtgatgg cgggacatct gaacggatat tttgacaaca ttctgatcat
     2161 gctcacaatg atggggatat tctggacgct ccggggctgt atgcttgcgg tcaacctggt
     2221 gcttgggctt ggaattcttg ttcatgagtc gatattgatc atgggttttc ccgccatatt
     2281 ccttgcccgt cattatgcgc ttatgggtga tgcgcctctg aaccggcgcg catggacgca
     2341 gtatctgctc aacatgctca ccccttttct gctgcctgtg ggactgttta tgttcctgtt
     2401 tgtctatcaa atggtgtttc tggataaaat aaggctgact caaatgctga cagcatatat
     2461 gaaagagttc ccgtttgttg ccgcgaatgc ggataccatt gtcccggaag catttgcgcc
     2521 ggattttgcg gcttattatc gcacgcagtc cccatggttc ttcaggcgcc ttttcagtcc
     2581 tcgctattta ataagcatga tgccgacgat ctgctgcatg agcgtttgcg tgttgcggat
     2641 ataccgtgca cgcttgaacg catattccat ttttctgctg gcggcggtat gcttctctcc
     2701 tcttcttatg catgcggttg cctgggatgt ggcgcgtctg tggacatatc cgatcatttc
     2761 gtcttttctt tgcatgtgga ttttgacaga agtgttggat gcgccgcaca cggccctggc
     2821 cggccgaaaa acatatatgc tgtccgccct tgcctttctg atgctcaaca tttttgtccg
     2881 gacgcctttg atggatgatc gacaggaacg gttttccgga ctcgtccgct tgtttctcta
     2941 tggccccgtg attatatatt tcctttgcat gacaatacgc atgttccagc gcaatcggca
     3001 gcaatccaaa atggaaccgc gaatagacgc aaataaacac gaatacaaag gaaaaaaaca
     3061 ctagcggata tgggcgtgca ttcgctgttc ctgattggtg gtcgaaatat ttgtgcacgg
     3121 acagtaaatg aaaattgttt tttggatttg tttgatcggt ctgttctata tttacatcgg
     3181 ctatccgctt ctgatctggg ttctggcgcg attgaagggg cgctctgtgc gcaaggctcc
     3241 ctggccggga aatttctcga tcatgattgt ggcacacaat gaggccgaaa acctgccgcg
     3301 gaagatcaaa agcattttgg agtccccggc ggcctcgcag attgaggaaa tactgatcgc
     3361 ctccgacggt tcgactgatg atacagccca agtggtccgg gatataaagt gcgatctggt
     3421 gcacctggtc gagtacgggg agcgccgcgg caaggcggcg gttctcaatg aggttttgcc
     3481 cgcctgcaag tcggagatcg tggtgctgat ggatgcccgt caaacagttg atccccaggc
     3541 atttgacgaa ctgctgtcga atttttcgga tgcctcagtg ggtgttgtca gcggtgagct
     3601 tgtttttcag aaaccgggca gtgaattctc tgatccggag aaggggttgg atgcctactg
     3661 gcggtatgaa aaattcatcc gcaaacagga aagccgtttc cggtcggttc ccggcgccac
     3721 gggcgcgctg tacgcgatca gaaaagagct tttcgagccc tttcctgcgg acgcgattct
     3781 ggacgacgtg gccatcccga tgtccattgt ccggcaggga ttccgctgta ttttcgatga
     3841 aaaggccctt gtttatgacc gaccgtcctc ctctccggag caggaagccg tccgcaagcg
     3901 ccggaccata gccgggaatg cgcagcttgc ggtgctgtgg ccgtggcttc t
//
LOCUS       DAOQWR010000183         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_23623_length_3931_cov_3.774768, whole genome shotgun sequence.
ACCESSION   DAOQWR010000183 DAOQWR010000000
VERSION     DAOQWR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23623_length_3931_cov_3.774768"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKK36_11140"
     CDS             <1..309
                     /locus_tag="PKK36_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95148.1"
                     /translation="AGNLHMRPEQFPELIELVPVSCRNQKFHAAENIRIRGRTPDKRR
                     PLPRRILSKLRVFAEMGVNCPCLGRSVRYACRELGRKEAALLPPLQETLLNQTLRTVG
                     "
     gene            374..3577
                     /locus_tag="PKK36_11145"
     CDS             374..3577
                     /locus_tag="PKK36_11145"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HNR95149.1"
                     /translation="MSATRKRDLILSFDIGHSSIGWNVLMNSPDGLAELGLGTVIFEA
                     DGCLASKRRDFRRQRRTIRSRRQRIEWLEKVFLTQGLLNPKQIEAKHNAGGGNSAPWL
                     LSAAVLAGKGKCVLSAEELFDVLRWYAHNRGYDGNRRWSGTADENKEDTEREQAALNL
                     MEKHGTTTMAETVSAQLGLAPDGHKRASMNYYKGRDIGAAFPRSVVTAEVKAILQIHA
                     GKLKGCTEEFMQAVCGEADEAWKAIQTEGIRMAKNFKGSLLFGQKVPRFDNRILSFCP
                     VSNTPTPLKSCREFMDYRCAMFLAAVRVAAAEDKALRALNAAELKELWDRIHLTGYMP
                     AGEFKKAVRKMTGCTRDNLDGLMVIEDAGTNLITDPALKLVHSNTVLKIIWPEIPEDA
                     SSALLARLRAKQRITVADILAGAGTATDGLNTAVDSAYKKERKKIPDKTRWIERKSFH
                     AEYDSGRAPYSRSVMTKAVQEVLSGKDPRAEGGCLYVTSEMSARQAGRSLDKMTNNHL
                     VRHRLLILNRLMKDILNEFAAGDKDRVETIVVEVNREVASMSGKTIKEIKTEEGRKRA
                     DHKRARKIMEDAVQEAGSDAEISAGLIRKARIAEDLGWTCPYTGKKYGPLDLIHKQVD
                     MDHIIPRSERLSDSMAGMVITFSEVNRWKDKRTAWQFVEQEQGKPVQGNPALTITSLN
                     AYREFVEKLKTAGGVDEDQARRKARKQYLLMPDYIEKSFTPRDLTISSYVTKMAVQQI
                     TNTFNTCKRKPQIVSLPGRLTGEARKSWKLTGLLHAVNSEISGSTLKDEVRGMTHLHH
                     ALDASVMGLLAANISDLSDGAVWQLLLKRHLTRDESRYLKEHTRRISIDSEGRPHLQD
                     IPKTITGQLQKLLAERRVVQHIPASMTGLHVKLNTHGILGMDDQTGKVILQQKKEGKH
                     KPELDSEHPVKVLGFAAPAGSKLKKQKGARIIDQNYGVALTLPEPQIIPWHKVWPRLN
                     EIREKTGKWPQVLRKGMIINVPKGRYKGTWKIFSVKNEQRYMALDMGAPDGCEATKRS
                     VNLTTLTRDSMQIIRTKLTGNGLCRITSSISTAPTSG"
     gene            3532..>3931
                     /locus_tag="PKK36_11150"
     CDS             3532..>3931
                     /locus_tag="PKK36_11150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNR95150.1"
                     /translation="MSYHIIHIDSPDIRLNCRDGQLICRGANGEKTLPMEDIAAVVIS
                     SFSASIHSEVLLQAAEKGVAFIYCRNFKPVSLVLPASRATDTALTRAVIGLDEKARAR
                     LWRATVDAKCGNQYALAEVFSPTSSSLEPMK"
BASE COUNT         1038 a         1085 c         1134 g          674 t
ORIGIN      
        1 gctggcaatc tccacatgcg ccccgaacag tttccggaac tcatcgagct tgtgcctgtt
       61 tcttgtcgca atcagaagtt tcatgccgcg gaaaatatac gcatccgcgg aagaacacca
      121 gacaaaagga ggccgctccc gcgccgcatc ctcagtaaat taagggtttt cgctgaaatg
      181 ggtgtgaatt gtccttgttt gggccggtca gtgcgctatg cttgccgtga actgggcaga
      241 aaagaggcgg cattgctgcc gcctcttcag gaaacgcttt taaatcagac gctgcgaaca
      301 gtgggctgat ctgcgttatc taactttgtc ttggttccaa acaagactgt catatgtgta
      361 tcaggaggcc gtcatgagtg caacaagaaa acgcgatctg atcctttcct tcgatatcgg
      421 gcacagctcc atcggctgga atgtgctgat gaactccccg gacggcctcg ccgaactcgg
      481 gctgggaacc gtaatcttcg aggcggacgg ctgccttgcc agcaagcgaa gagatttccg
      541 cagacaacgg cgcaccatac gctcccgcag acagcggatc gaatggctcg aaaaagtttt
      601 tttgacgcag ggtcttttga accccaaaca gatagaggcg aaacacaacg ccggaggagg
      661 aaacagcgcg ccgtggctcc tgtcagcggc cgtgctggcc ggaaaaggca aatgcgtgct
      721 gagcgccgaa gaactgtttg atgtactgcg ctggtatgcg cacaaccggg gttatgacgg
      781 caaccgtcgc tggtccggaa ccgcggacga gaacaaggaa gacacagaga gagagcaggc
      841 cgccctgaac ctgatggaga aacacggcac cacaacaatg gccgaaaccg tcagcgccca
      901 gctaggactg gcacccgacg gccacaagcg ggcttccatg aactattaca agggccgcga
      961 cataggggcc gcgtttcccc gcagtgtggt cacagcggaa gtgaaagcca tactgcagat
     1021 tcatgccgga aaactgaagg gctgcacgga agagtttatg caggccgtgt gcggagaagc
     1081 agatgaggca tggaaagcca tacaaacaga aggaatccgc atggcgaaaa acttcaaggg
     1141 cagcctgctt tttggccaga aggttccgcg gtttgacaac cgcattctgt cattctgccc
     1201 ggtctcaaac acgccgaccc cgttgaagtc ttgcagggaa ttcatggact accggtgtgc
     1261 gatgtttctt gccgcagttc gtgtggcggc cgcagaggac aaagccctgc gggcgctgaa
     1321 tgcggccgaa ctgaaggaac tgtgggaccg tattcacctg acaggctaca tgccggccgg
     1381 agaattcaaa aaggccgtcc gcaaaatgac gggctgcacc cgggacaatc tggacggact
     1441 gatggtgatc gaagacgcag gcacgaacct gatcaccgat ccggcgctta agctggtgca
     1501 ttcaaacacc gttcttaaaa taatctggcc ggaaatcccg gaagatgcct ccagtgccct
     1561 tctcgcacgg ctgcgggcaa agcagagaat aaccgtggcg gatattctgg ccggggcggg
     1621 aaccgcgaca gacggcctca acaccgcagt agattccgcc tataaaaagg agcgcaagaa
     1681 gattcccgac aaaaccagat ggatcgagcg taaatcattt catgccgaat atgacagcgg
     1741 acgcgccccc tactcccgct cagtcatgac caaggcggtg caggaggttc tgagcgggaa
     1801 agacccccgg gcagaaggag gctgcctcta tgtaacaagc gaaatgagtg caagacaagc
     1861 gggccgcagc ctggacaaga tgaccaacaa tcacctggtc cggcaccggc ttctgatttt
     1921 aaacagactg atgaaagaca tcctgaatga gtttgcggcg ggagacaagg accgggtcga
     1981 aaccatagtg gtggaagtca accgcgaggt cgcctcgatg tccggtaaaa ccataaagga
     2041 gatcaagacc gaagaaggcc ggaagagggc cgaccacaaa cgggcccgca agatcatgga
     2101 agacgccgtg caggaggcgg gcagcgatgc ggaaatctca gccggcctga tccgcaaggc
     2161 gcgaattgca gaggatctcg gctggacctg tccctacaca gggaaaaaat acggtccgct
     2221 cgatttgatt cacaagcagg tggatatgga tcacatcatc ccccgttccg agcgcctgag
     2281 cgactccatg gcgggaatgg tgatcacctt ctccgaggtg aaccggtgga aggataaacg
     2341 gaccgcatgg cagtttgtcg agcaggagca gggcaagccc gttcagggaa atcccgccct
     2401 cacgatcact tcgctgaatg cctaccgtga gttcgttgaa aagctgaaaa ccgcgggcgg
     2461 ggtggacgag gatcaggccc ggcgcaaagc ccgaaagcag tatttactga tgcctgacta
     2521 tattgaaaaa agcttcaccc cacgcgacct gaccatcagc tcctacgtca ccaaaatggc
     2581 ggtgcagcag ataacaaaca ccttcaacac atgcaaacgc aaaccgcaga ttgtatccct
     2641 tccggggcga ttgacgggtg aagcaagaaa aagctggaag ctcacgggcc tcctgcatgc
     2701 cgtgaacagt gaaataagcg gaagtaccct taaggatgaa gtccggggca tgacgcacct
     2761 gcaccatgcg cttgacgcat ccgtaatggg cctgctggcc gcaaacatca gcgacctgag
     2821 tgacggcgcg gtgtggcagc tcctcctaaa gcggcatctc acgcgcgatg aaagccgcta
     2881 cctgaaagaa cacacccgaa ggatcagcat cgactccgag gggcggcccc acctgcagga
     2941 catcccgaaa acgataaccg gacagctgca gaagcttctc gccgaacggc gcgtggtcca
     3001 gcacatcccc gccagcatga ccggactcca tgtgaaactt aacactcatg gtattctggg
     3061 catggatgac caaaccggca aagtgatcct tcagcagaaa aaagagggca agcacaagcc
     3121 cgaactggac agcgagcatc cggtgaaggt tctgggcttt gccgctccag cgggctcaaa
     3181 gctgaaaaaa caaaaagggg cgcgcatcat agatcagaac tacggcgtcg cattgaccct
     3241 gcccgagccg cagatcatcc catggcataa agtctggccc aggctcaacg agatcagaga
     3301 gaaaaccgga aagtggccgc aggtgctgcg caaaggcatg attatcaatg tcccgaaagg
     3361 tcggtacaaa ggcacatgga aaatcttttc tgtgaagaac gaacaaagat atatggcgct
     3421 agacatgggc gcgcctgatg gctgcgaggc aacgaaacga agtgtaaatc tgacaacttt
     3481 gactcgcgac agcatgcaga taataagaac aaaactgacc gggaatgggc tgtgtcgtat
     3541 cacatcatcc atatcgacag ccccgacatc cggctgaact gccgcgacgg gcagttgata
     3601 tgccgggggg cgaacggcga aaaaacgctt cccatggagg acatcgccgc agtggtgatt
     3661 tccagctttt cggccagcat ccacagcgaa gtgctgctgc aggccgccga aaagggcgtg
     3721 gccttcattt actgccgcaa cttcaagccg gtcagccttg tgctccccgc cagccgcgca
     3781 accgacaccg ccctcacaag ggcggtgatc ggtctggatg aaaaagcacg ggcccggctc
     3841 tggcgggcca ccgttgacgc gaagtgcggc aaccagtatg cccttgcaga ggtgttctcg
     3901 cccaccagca gcagtctcga accaatgaag a
//
LOCUS       DAOQWR010000184         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_23802_length_3909_cov_3.127919, whole genome shotgun sequence.
ACCESSION   DAOQWR010000184 DAOQWR010000000
VERSION     DAOQWR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23802_length_3909_cov_3.127919"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..2755
                     /gene="cas3"
                     /locus_tag="PKK36_11155"
     CDS             272..2755
                     /gene="cas3"
                     /locus_tag="PKK36_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNR95151.1"
                     /translation="MKWGQCWAKTTPEGKPGIRVSTHGIIAGHVAKELCSLQRGSEAC
                     ATDFFPFTVAAHDVGKVSPGFQKKYFNAALSEINPGLASASSEAQGWTTNHAEVGEAA
                     FAAWLAARNVPGGKTWAEAIGIHHGMRNDPKQEGCAIYGGGEWAAERNELLDHLHKYF
                     NPPLPKHSLTTATFKILAGLTCVADWIASNEEFFPGNNLPGEKELRPKVADALADSGW
                     RNLRYRANLDFQKVFGFPPNETQGRFIECVDCPGIYVLEAPMGSGKTEAALFAAYKLL
                     VSGKNTGLYFGLPTRLTSDRIHIRVESFMNRILEDPGFVKLVHGHAWLNLESRAAEFR
                     AGMSWFHPRKRALLAPLGVGTIDQALMSVLKVKHHFVRTFGLAGKVVILDEVHSYDAY
                     TGALLDCLVEELARIGCTVIILSATLTQERRAAFVAGAPETRAYPLITSGVKTVEPKP
                     PENRNVPLTFFEKPNDELIERAVAEAERGLCVLWIANTVAQSQDVFARINGARRQGTF
                     CAGLLHSRFPAFRRTELEDEWMELLGKGGTRPPGCVLVATQVVEQSVDIDADLLITEL
                     APTDMLLQRMGRLCRHSRGGRPKGSGRAWIYGPTRTEFDNAETFKEALGPSGYVYAPY
                     VLWKTLQVWRDCPEIILPSQIRELLEATYADEHSTADWVQELKTELDTRKAKLQGAAL
                     GLTRIYCGKDDEDSARTRYSSRPTIQTLILDKCDNRVNQADIVLCDGTQLTLSAGEKD
                     FKKAVAIHKNMVSLPVRRPLDATVPTWLTALVFGPVAVLKKDGDKLFDLAGTEMPWGY
                     HREKGVYGLEPCGGCDSAREPEDDDESYY"
     gene            2739..>3909
                     /gene="casA"
                     /locus_tag="PKK36_11160"
     CDS             2739..>3909
                     /gene="casA"
                     /locus_tag="PKK36_11160"
                     /inference="COORDINATES: protein motif:HMM:NF021030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HNR95152.1"
                     /translation="MNLITDPWIPVVYGNGESLLVGLDQLIRESETIRDLNASPPQRI
                     ALMRLLLCITQRALDGPRDEDDWKNCRPRIIPASLDYLDSRKERFELHGDRPFLQAKT
                     LQATNNEPVEKLDASLSSELFNHTVTRDLSNADIALNLLTTQCFSLGGTIGVTLWDGY
                     TTNNFTKAVAAKGPGPSSDAPCAPSSMLHSALRGSCMLDSIFLNLLTKSEIPYDWGLP
                     IWEAATSSPVELQNEFTGTYLGRLVPLSRGILIDPGSRKCTYVAGLKYESLPNYREPM
                     GTVLLNRENEVFYMGIRLGQHVWRELGSILSLNAKSDAQGALALKKAGQAGMKDFDLW
                     VGGVSRKPGQNLIYDMLEWNFLLPVALLGASSLKKYADGVAMAARCEVALKDAIQK"
BASE COUNT          935 a          984 c         1151 g          839 t
ORIGIN      
        1 gctggcaatc tccacatgcg ccccgaacag tttccggaac tcatcgagct tgtgcttgtt
       61 tctcgtggca atcagaagtt tcatgccgcg gaaaataacc gcataacgcg cagaacgcca
      121 gacaaaaggc gaaacagacc gctgtcgtgt ttatgcagaa ctttgccttg ctcagagaaa
      181 cccgttgcca tattctgaac cggaataagc caggtcaaca atgccgctgt tcaaaaagca
      241 gaaagaatct gagctgaacg cctgccccgc gatgaaatgg gggcaatgct gggcgaagac
      301 cacgccagag ggtaaacccg gcataagggt ttctacccat gggataatcg cgggacatgt
      361 ggccaaagaa ttgtgttcat tgcagcgggg ttcagaggcc tgcgccactg attttttccc
      421 gtttaccgtt gctgcccatg atgtggggaa agtctcgccg gggtttcaga aaaagtattt
      481 caacgcggct ttgtctgaaa tcaatccggg gctggcatct gcttcttcag aggcacaggg
      541 ctggacgaca aatcatgcgg aggttgggga ggctgctttc gcggcgtggc tggctgcccg
      601 gaacgtgccc ggaggaaaaa cctgggcgga agccattggc atccatcacg ggatgcggaa
      661 tgacccaaaa caggaggggt gcgccatata tggggggggc gaatgggccg ccgaacgcaa
      721 cgagcttctt gatcacctcc acaaatactt caatcccccc ttgccgaagc actcgctgac
      781 cacggcaacc ttcaagattc tggcgggact tacatgtgtt gcagactgga ttgcatcgaa
      841 tgaggaattc ttccccggaa acaaccttcc cggcgaaaaa gaattgcgcc cgaaagttgc
      901 cgatgcgctt gctgacagcg gatggcggaa tttaaggtat cgagcaaatc ttgattttca
      961 gaaggttttt ggattcccgc caaatgagac gcagggccga tttattgaat gcgttgactg
     1021 tcccggaatt tacgttctgg aagcccccat gggaagcggg aaaacagagg ccgcgctttt
     1081 tgcggcctat aagctgctgg tgtccggcaa gaacaccggc ctctatttcg gactgccgac
     1141 ccggctgacc agtgatcgga ttcatatccg cgtggaatca ttcatgaacc gcattcttga
     1201 agatcccggc tttgtgaagc tggtgcacgg tcatgcatgg ctgaatctgg agtcgcgggc
     1261 ggctgagttc agggcaggga tgagctggtt ccatccccga aaacgggctt tgctggcccc
     1321 ccttggcgtg gggaccattg atcaggcact gatgagcgtc cttaaagtga aacatcattt
     1381 tgtgagaaca ttcggcctgg ctggtaaagt ggtgatcttg gatgaggttc acagttatga
     1441 tgcttatacc ggagcactac tggactgcct ggtagaggaa ctggcccgca ttggctgcac
     1501 cgtgatcatc ttgtcggcaa cactcacgca ggagcgccgc gcagcattcg tcgccggggc
     1561 gccggagaca cgcgcctatc cactgatcac ttccggggtc aaaaccgttg agccaaagcc
     1621 gcccgaaaac agaaacgtgc ctcttacatt cttcgagaag ccaaatgatg aactgattga
     1681 aagggctgtg gccgaagcgg aacgcgggct gtgtgtgctg tggatcgcca acaccgtggc
     1741 gcaaagccaa gacgtctttg cgcgaatcaa tggggcccgc aggcagggca ctttttgcgc
     1801 cggtcttctg cattcccgct tccccgcatt ccgaagaaca gagctggaag acgaatggat
     1861 ggaattactg ggaaagggag gaacccgtcc gccgggttgt gtactagtcg cgacgcaggt
     1921 ggttgaacaa agtgtggata ttgatgctga tctgctgatc accgagcttg cgccaaccga
     1981 catgctgttg cagcgcatgg gccggttatg ccggcacagc agaggcgggc ggccgaaagg
     2041 ttcgggtcgg gcatggattt atggaccaac cagaacagaa tttgacaatg ccgagacgtt
     2101 caaggaagcg ctgggcccaa gcggttacgt ttacgcgccc tatgtgctgt ggaaaacact
     2161 tcaggtgtgg agagattgtc cagagatcat cctgccctcg caaattcgcg aactgctgga
     2221 ggcgacctac gctgacgagc acagcacagc ggattgggtg caggaactca agaccgaact
     2281 ggacacaagg aaggctaaac tgcagggagc ggcgttgggc cttacgcgca tctactgcgg
     2341 caaggatgat gaggactcgg cccgaacccg atacagctcg cgcccaacca ttcaaacact
     2401 catactggac aagtgtgaca accgggtaaa ccaggccgat attgttttgt gtgacgggac
     2461 ccaattgacg ctttccgccg gagagaaaga cttcaagaag gctgtggcga ttcacaagaa
     2521 catggttagt cttccggttc ggagacccct tgatgcaact gtaccaactt ggttgacagc
     2581 gctggtattc ggacccgtgg ctgtgctaaa aaaggacggc gacaagctgt ttgatttggc
     2641 cggaacggaa atgccgtggg gatatcatcg ggaaaaaggc gtttatggac tagagccgtg
     2701 tggcggatgt gattctgcac gggaaccgga ggatgatgat gaatcttatt actgaccctt
     2761 ggataccggt agtttatggg aatggagagt cgcttctcgt cggactggat cagcttattc
     2821 gcgaatcaga gaccatccgc gatctgaacg ccagcccgcc gcagcgcatt gccctgatgc
     2881 gcctgctgct gtgcatcaca cagcgcgcgc ttgatgggcc cagagatgaa gatgactgga
     2941 aaaactgccg gccgcgcatc atccccgcca gtctcgatta cctcgacagc cgaaaggaga
     3001 gatttgaact ccacggggac cggccctttt tgcaggcaaa aacccttcaa gcgacaaaca
     3061 atgagccagt tgaaaagctg gatgcgagct tgtccagcga actttttaat cacacggtca
     3121 caagggattt atcaaatgcg gatatcgcat taaacctgct cacaacacag tgtttttcac
     3181 tgggaggaac aatcggtgtc acgctgtggg atggatacac aaccaataat tttacaaagg
     3241 cagtggcggc aaaaggcccc ggtccgagtt cggatgcccc ctgtgccccg tcgagcatgc
     3301 tgcattccgc actgcgagga tcttgcatgc tggattctat cttcctgaat cttctgacca
     3361 aatcagagat cccgtatgat tggggactgc cgatctggga ggccgcaacc agttcaccgg
     3421 tcgaactgca gaacgaattc acaggaacct atctgggccg gcttgtcccg ttaagcaggg
     3481 gcatcttgat tgatcccggc agccgcaaat gcacctatgt ggccggattg aaatatgagt
     3541 cattgccgaa ctaccgggaa cccatgggga cggtcttgct gaacagggag aatgaggttt
     3601 tctacatggg aatccggctg ggacaacatg tttggaggga attgggttcg attctctctc
     3661 ttaacgccaa gtcggatgca caaggagcgc tggcgttgaa gaaagccggg caggcgggaa
     3721 tgaaagactt tgatttgtgg gtgggcggcg tcagccggaa gccgggacag aacttgattt
     3781 acgacatgct ggaatggaac ttcttgctcc ctgttgcttt gcttggggct tcttccttga
     3841 agaaatatgc cgatggtgtc gcgatggcgg cccgttgcga ggttgcattg aaggatgcta
     3901 ttcagaaga
//
LOCUS       DAOQWR010000185         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_25026_length_3779_cov_4.869227, whole genome shotgun sequence.
ACCESSION   DAOQWR010000185 DAOQWR010000000
VERSION     DAOQWR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25026_length_3779_cov_4.869227"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKK36_11165"
     CDS             complement(<1..256)
                     /locus_tag="PKK36_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007393326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNR95153.1"
                     /translation="MNTLLQSIAAGRAVAREDLGPLLALFEPGDQEALFKAAYAMKLK
                     QVGPKVYFRGIIEYSNICEKNCYYCGIRRDNRKVRRFQMTR"
     gene            complement(258..1682)
                     /gene="hydG"
                     /locus_tag="PKK36_11170"
     CDS             complement(258..1682)
                     /gene="hydG"
                     /locus_tag="PKK36_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNR95154.1"
                     /translation="MKSWPEMKLSEGARDFIDDAALTALIENARPDPVRVREILAKSL
                     SKQALTVAETAVLLAADSPDLVEEIFSTARELKQTVYGNRIVLFAPLYIGSMCVNDCT
                     YCAFRRSNDGAVRRTLSLEDVREQTLALERVGHKRLILVYGEHAEYDADFIAATVRKV
                     YETKEGHGEIRRVNINAAPLDAEGYRKVKDAGIGTYQIFQETYHHETYRRVHPGKTRK
                     GNYLYRQDGLSRAFEAGCDDVGIGALFGLYDWRFEVLGLVSHALYLQQRYGVGPHTIS
                     FPRLQPAHGAVTKTEHLVSDEEFKRVIAILRLAVPYTGLILTARERAELRREVMSFGV
                     SQIDAGSRIELGGYTEVGDAQVMEKEQFSLGDIRSLDEVMRELIQDGYVPSFCTACYR
                     LGRTGEHFMEFAIPGFIKRYCTPNALSTLKEYLVDYASSETKAAGEALIRDELARFEE
                     GEMKNKLVGRLLAIEAEGKRDLYF"
     gene            complement(1685..2812)
                     /locus_tag="PKK36_11175"
     CDS             complement(1685..2812)
                     /locus_tag="PKK36_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNR95155.1"
                     /translation="MEPLCVMVVDDELGMRAGAERALRDYRVKMPDGNEEISFRVLSA
                     DSGEEALQIISQTPPDILLLDYKLPGMTGLDLLVKLAAGSGDMLVIMITAYASIETAV
                     QATRQGAYDFLPKPFTPEELRDVIRRSAMHVVLTRQARQMAEEKKRVRFEFIRVLGHE
                     LKAPLAAVEGYVNIMKDESLGPELAPYKEMISRSHVRLEQMKKLIVDLLDMTRIEAGQ
                     KVRHVEQMDICAAFRDAVELVKPAAKERGIRVNVKCPESCSVAADRSEMDMIFSNLLT
                     NAVKYNRDNGSVEAVLTPGEGQIEIRVTDTGIGMSPEEQEKLFGEFVRIKNDQTRNIL
                     GSGLGLSIIKRLVELYRGSITVISEAGKGSTFTAVLKLEKA"
     gene            complement(2814..3218)
                     /locus_tag="PKK36_11180"
     CDS             complement(2814..3218)
                     /locus_tag="PKK36_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR95156.1"
                     /translation="MSEKGPARKKALLVDDDMDFRLQQRMLLESMGLEVVEAETRAQA
                     EKILDETRPDIAVIDLMMEETDSGFVLCHRLKRHNPELPVIIVTAVASETGMAFSAST
                     REERSWIKADAVLNKPVRPAQLRKEIDRLLKA"
     gene            complement(3215..>3779)
                     /locus_tag="PKK36_11185"
     CDS             complement(3215..>3779)
                     /locus_tag="PKK36_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR95157.1"
                     /translation="DSVMIAEQADRCKRIVAGLLNFARQNKVLRQPVDVRHLIGQSVK
                     LISIPDTVKVSVAEQTGNPVIDVDGDQIVQVISNLVSNAVAAMPNGGELKIGASGDDS
                     HIRITVEDTGIGISEQNAKKVFEPFFTTKQMGRGTGLGLAVAYGIVKMHRGSITLESN
                     AKPEAGPTGTIFTVTLPRSVAQTEETI"
BASE COUNT          719 a         1195 c         1040 g          825 t
ORIGIN      
        1 cgcgcgtcat ctggaaacgg cggacctttc tgttgtcccg gcgaatgccg cagtagtagc
       61 agttcttttc gcagatgttg ctgtactcga taatgccgcg aaaatagacc ttggggccaa
      121 cctgcttcag tttcatcgca taggccgcct tgaacagggc ttcctgatcc cccggttcaa
      181 acagcgcaag cagcggcccg aggtcctccc tcgcaaccgc ccggccagcc gcgatgcttt
      241 gcaggagtgt gttcatgtca gaaatacaga tcgcgcttcc cttcggcctc gattgcaaga
      301 aggcgcccga ccagtttgtt tttcatttcg ccttcctcga aacgggccag ctcatcgcga
      361 atgagcgcct cgccggcggc ctttgtttcg gagctggcat aatccaccag atattccttg
      421 agggtggata gcgcattcgg agtgcagtag cgcttgatga atccggggat ggcaaactcc
      481 atgaagtgct cgccggtgcg gccgagccgg tagcaggccg tgcagaaact gggcacatag
      541 ccgtcctgga tcagctcgcg cataacctcg tccagggagc ggatatcgcc cagggaaaac
      601 tgttcttttt ccatgacctg ggcatcgccc acttcagtgt atccgcccag ttcaatgcgg
      661 cttccggcgt caatctgcga gactccgaag gacatcactt cgcggcggag ttcggcgcgt
      721 tcgcgcgcgg tgagaatcag tccggtatac ggcacggcaa gccgcagaat ggcaatcacg
      781 cgcttgaact cttcatcgct cacgaggtgt tccgttttgg taacggcccc gtgcgcaggc
      841 tgcaggcgcg gaaaacttat agtgtgcggc cccaccccgt agcgctgctg aagatacagc
      901 gcatggctca ccagccccag cacctcgaac cgccagtcat agagcccaaa aagggcaccg
      961 ataccgacat cgtcgcatcc ggcctcaaag gctcggctga gcccgtcctg acgatacagg
     1021 taattgccct tgcgggtctt gccgggatga acccggcggt aggtttcgtg atgataggtc
     1081 tcctggaaaa tctgataggt gccgatgccc gcatccttca ccttgcggta gccctcggca
     1141 tccagcggcg cggcattgat gttgacccgc cggatttcac catggccctc ttttgtttca
     1201 tagactttgc gaaccgttgc ggcaatgaag tcggcatcgt attccgcatg ctccccgtac
     1261 accagaatca gccgcttgtg cccgacgcgc tccagcgcaa gagtctgctc gcggacatcc
     1321 tccaggctca gcgtccggcg aacggcgccg tcgttgctgc ggcggaacgc gcagtaggtg
     1381 cagtcgttga cacacatgct cccgatatag agcggggcaa aaagcacaat ccggtttcca
     1441 tagacagttt gcttgagctc gcgggccgtg ctgaatatct cctcgaccag gtccgggctg
     1501 tcggccgcga gcaatacggc ggtttccgcc actgtcagcg cctgcttcga aagacttttg
     1561 gcgagaattt cgcgcacccg caccggatca ggccgggcgt tttcgatcag cgcagtcagc
     1621 gcggcatcat caatgaagtc gcgcgcgccc tcgctcagtt tcatctccgg ccaggatttc
     1681 atggttaagc cttttccagt ttcaggaccg ccgtaaaggt gctgcccttc cctgcctcac
     1741 ttataacagt aatgcttcct ctgtaaagtt caaccagccg cttgataatg ctcaagccca
     1801 gcccgctgcc aaggatgttg cgggtctggt cgttctttat ccgaacgaac tccccgaaca
     1861 gtttctcctg ctcctctgga gacatgccaa tgccggtgtc cgtcacacgg atttcgatct
     1921 gtccctcacc cggcgtcagc acagcctcaa cggacccgtt gtcccggttg tacttgaccg
     1981 cattggtcag caggttgctg aagatcatgt ccatttcgct gcggtcggcc gcaacggaac
     2041 agctttcagg gcacttcaca ttcaccctga tcccgcgttc cttcgctgcc ggcttcacca
     2101 gctcgacggc atcgcgaaag gccgcgcaga tatccatctg ttccacatgc cggactttct
     2161 ggccggcctc aattcgcgtc atgtccagca gatccacgat cagctttttc atctgctcca
     2221 gacgcacatg gctgcggctg atcatctcct tgtagggcgc aagctccggg ccgagcgact
     2281 catctttcat gatgttcaca taaccttcga cggcggccag aggagccttg agttcgtggc
     2341 ccagcacgcg gatgaactcg aagcggacac gtttcttctc ctcggccatc tggcgggcct
     2401 gacgcgtcag cacgacgtgc atggcggaac ggcggatcac gtcgcgcagc tcctcgggcg
     2461 tgaagggctt gggcaggaag tcgtaggcgc cctgccgcgt ggcctggacg gctgtttcaa
     2521 tggaggcata ggccgtgatc atgatcacca gcatgtcgcc cgaacccgcg gccagcttca
     2581 ccagcaggtc gagaccggtc atgccgggga gcttgtagtc cagaagcaga atgtccggag
     2641 gcgtctgact gatgatctga agcgcctcct cgcccgaatc cgccgaaagc accctgaagg
     2701 aaatctcctc attgccgtca ggcatcttga cgcggtaatc gcgaagcgcg cgttctgcgc
     2761 ccgcgcgcat tcccagttca tcatccacaa ccatgacaca tagcggttcc atattatgcc
     2821 ttcagaagcc tgtcgatttc cttgcgcagc tgcgccggac gcaccggttt gttcagcacc
     2881 gcatcagcct tgatccagct tcgctcctcg cgtgtcgagg ccgaaaacgc catgccggtc
     2941 tcgctggcca ccgcagtgac aatgatcaca ggcagttcgg gattatgcct tttcaggcga
     3001 tggcaaagca caaagccgga atcggtctcc tccatcatca ggtcaatgac ggcgatgtcg
     3061 ggacgggttt catccagaat cttctcggcc tgcgcgcggg tctcggcctc gacaacctca
     3121 aggcccatgc tctcaagcag catgcgctgc tgcaggcgga aatccatgtc gtcatccacc
     3181 agcagcgctt tttttcttgc cggtcctttt tcgctcatat tgtctcctct gtctgcgcaa
     3241 cggaacgggg cagtgtcacg gtgaagatcg tgccggtggg cccggcctcc ggcttcgcat
     3301 tggactccag cgtgatggac ccgcggtgca tcttcacgat gccgtaggcc accgcaagac
     3361 ccagcccggt tcccctgccc atctgtttgg ttgtaaaaaa cggttcaaac accttcttcg
     3421 cgttctgttc ggaaatgcca atgccggtgt cctcaaccgt aatgcggata tgggaatcat
     3481 cgcccgacgc accgattttc agctctccgc cgttcggcat cgccgccacc gcattgctga
     3541 caagattgct gatcacctgc acaatctggt cgccgtccac gtcaatcacg ggattccccg
     3601 tctgctccgc aacggacact ttcaccgtat cgggaatgga aatgagcttc acgctctgcc
     3661 cgatcaggtg gcggacatcc acgggctgac gcaggacctt gttctggcgg gcaaagttga
     3721 gaagtccggc cacgattctt ttgcaccggt ctgcctgctc ggcgatcatg acggagtcg
//
LOCUS       DAOQWR010000186         3745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_25375_length_3745_cov_5.626016, whole genome shotgun sequence.
ACCESSION   DAOQWR010000186 DAOQWR010000000
VERSION     DAOQWR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25375_length_3745_cov_5.626016"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..820
                     /locus_tag="PKK36_11190"
     CDS             383..820
                     /locus_tag="PKK36_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95158.1"
                     /translation="MISAAQGPEMPSDTPPPAVVEGSDSETAGNGQSLVVQHEGLRDP
                     FWPVGYSPRSEKREEETKAIEEEQADLAADEELWNTLQKKLRIAGVAVVGDKKMATIN
                     NRLVEEGGIVGIRHENREFRWKISEITDSRRVRFTRLDIIPQP"
     gene            complement(833..2410)
                     /locus_tag="PKK36_11195"
     CDS             complement(833..2410)
                     /locus_tag="PKK36_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95159.1"
                     /translation="MSVNVPRRLWFWILAAAFAWAMFFWVTHVPYRPDRLFRAIPANS
                     AWISVHDGLAVRWAGLSGNPLIMTVLNACGIDLETQAMLAADKDFQYWFRRLAPDRAV
                     TAFVPSFSRQGQQAWVVASWLGGDSQRLRWLLTWTGQHPCRREGRHQGRILWALDTGD
                     GRTPFRFALVEGGVIGVLCDDPAALRALLDMYDGRLPSAASVIEAFMGNSMSLSPSAR
                     DFGLFNAGALPMSFALDNVTPDGLTLSMAAPLPLQVPADWLEEGQYDELVRVIGDVPL
                     AALLVSRQALLSALEHPLAALPASAARYWISRMDGESLMLSVLGGEYSGRIRGIKWPA
                     LLAGVPIGEAAEAREVMQPLLDDLNRTYQWGLILHEIPQGDQPAIYIIESTAGNAYGK
                     LNLQEQMACTVRGGWLLLASNSGTLMKICGRYSRGEQGASAGWIRPVPGDAALCRLDL
                     AGGGKIVKLALGAYSLKLLFSDPDGTQEERQRINEYKAWIDMLAPFGQLRARVYSDNE
                     QAILKLHMGTGSGISDN"
     gene            complement(2429..3460)
                     /locus_tag="PKK36_11200"
     CDS             complement(2429..3460)
                     /locus_tag="PKK36_11200"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNR95160.1"
                     /translation="MTIQDCGMLLGGDQDLLRKNHEQFLAQPVDLDLSGEVYVPFGEF
                     QRTISTPVEVRGPGTFFGKATRTIRFEPTDKEGWWVRRSDLPDCLPTEVSIRNVWTTG
                     EVVSNIVLRSGSPHNYVRMVEHIIALKAGLGIDNLCVVLDSGDPPIFNRGSLDLVEAL
                     EKACITNTSARARYVTVKEPVALVAPHGGFLLLAPCPGGPPRLDLDVAIAFPNAIGQQ
                     RIRLPVTYDHFRKGAEARTNTTAAKKIWCQTIGKIFADVRNLGYTEENVLVAGRHRYV
                     NEPHLLHGDKSLEAAWHRACLDLMAAVSLIDAGRFVGRITSYKAGHALDVQMITRLYL
                     NDLLTEYEG"
     gene            complement(3482..>3745)
                     /locus_tag="PKK36_11205"
     CDS             complement(3482..>3745)
                     /locus_tag="PKK36_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95161.1"
                     /translation="ILTAAIAVVGFITIAHTYIQEQKVLKGLLVVCSYCGKIRLNDQA
                     WERLDFYVQDRTLAEFSHGVCPDCMKKAMQSELGSSASMPPRN"
BASE COUNT          858 a         1160 c         1001 g          726 t
ORIGIN      
        1 ccataatcca gaccccagat cgcgccccaa aagcaaagtt gatgaataga tcaggctaaa
       61 cctatacgat taaaaggttt tatttcagat ttctgctgtc agacaaacca cgaactggag
      121 ccatatgcct aaggtcgttc acgccgcccc ctgttcttag tggcgtcgaa agcaggaaat
      181 cggcacaaaa caggcgcttg gggcctgtct ccggcagctg ttcagagccc ggtcaggcga
      241 cgttgctcag gaaaatgctt ttctccattc agtttctggg ttatgttaac gcctgtgaaa
      301 gtgaatggtg ttcgtagtaa atattgaggg gtttggaatg aaaatcctgt tctgcccgtt
      361 tatgcttctc ctgatttcaa ccatgatttc agccgcgcag ggaccagaaa tgccgtcaga
      421 caccccgccc ccggccgtag tggaagggtc tgattcagaa acagccggca acggccagag
      481 ccttgtggtt cagcatgaag gtcttcgtga tccattctgg cctgtgggat actcgccccg
      541 ttcagaaaaa cgggaggaag aaaccaaggc aatagaggaa gagcaggccg atctcgccgc
      601 ggatgaagaa ttgtggaaca ctctccagaa gaaactgaga atcgcggggg ttgctgtcgt
      661 gggcgacaag aaaatggcca ctatcaataa tcgcctggtt gaagagggcg gaattgttgg
      721 aattcgtcat gaaaatcggg aattccgctg gaaaatttct gaaatcaccg attcccgtcg
      781 cgtacgtttc acgcgccttg atatcatccc tcagccttga gtttcagggg tctcagttgt
      841 cagagatgcc ggatcccgtc cccatgtgca gcttcagaat cgcttgttca ttatccgaat
      901 aaacacgggc ccgcagctgg ccaaacggag ccagcatgtc gatccatgcc ttgtactcat
      961 tgatgcgctg acgctcctcc tgtgtgccgt ccggatcact gaaaagaagc ttgagcgaat
     1021 aagcgcccag ggccagcttc actattttcc cacccccggc cagatccagg cggcagagcg
     1081 ccgcatcccc cggcaccggc cggatccagc ctgctgatgc gccctgctct ccccggctgt
     1141 agcggccgca aatcttcatc agtgttcccg aattgctggc cagcagcagc catccgcctc
     1201 gaaccgtgca ggccatttgc tcctgcagat tgagcttccc ataggcattc cccgccgtgc
     1261 tttcgatgat atatatggcc ggctgatccc cctgaggaat ctcgtggaga ataagccccc
     1321 actgataagt ccggtttaag tcatccagga gcggctgcat aacttcacgg gcttccgcgg
     1381 cttccccgat cggcacacca gccagcaggg cgggccactt gatccccctg atacgcccgc
     1441 tgtactcccc tcccagtaca gacagcatca gtgactctcc gtccatcctg cttatccagt
     1501 agcgagcggc agatgcgggc aaggccgcaa gaggatgctc cagcgcggac agcagcgcct
     1561 gccgcgacac gagcagcgcc gccagtggga catccccgat cacccggacc agctcatcgt
     1621 actgcccctc ctccagccag tccgcgggaa cctgaagcgg gaggggtgca gccatggaaa
     1681 gagtcaggcc atccggcgtg acattatcca gtgcaaagct catgggcaag gcccccgcgt
     1741 taaagaggcc gaaatcccgg gcagatggcg acagagacat ggagttcccc ataaaagctt
     1801 caattacaga agccgcgctg gggagccggc cgtcatacat atccagcagc gcccgcagcg
     1861 cggccggatc atcgcagagt accccaatca ccccaccctc aacgagagca aaacgaaacg
     1921 gagtccgtcc atccccggta tccagagccc aaagaatgcg gccctgatgg cggccctcac
     1981 gccggcacgg gtgctgcccg gtccaggtga gcagccagcg cagccgctga ctgtcccccc
     2041 ccagccagct ggcgaccacc catgcctgct gcccctgacg ggaaaaagag ggaacaaagg
     2101 ccgtgaccgc gcgatccgga gccaggcgcc gaaaccagta ctggaagtcc ttatctgccg
     2161 ccagcatggc ctgtgtttcg agatcaatgc cgcacgcatt gagcactgtc ataatgagcg
     2221 gatttccgga aaggccggcc cagcggacag caagcccgtc atgaacactg atccatgcgg
     2281 aattggccgg aatcgcgcgg aagagcctgt ccgggcgata cggcacatgc gtgacccaga
     2341 agaacatcgc ccacgcaaag gcagccgcca gaatccagaa ccataaacgc ctgggaacat
     2401 tcacgctcac acacagctcc aacggcggtc agccttcata ttcggtcagg aggtcgttta
     2461 aataaagccg ggtgatcatc tggacatcca gcgcatggcc cgccttgtac gaggtgatgc
     2521 gtccgacaaa acgtccggca tcaatcaggg aaaccgccgc catcagatca aggcaggccc
     2581 tgtgccaggc ggcctcaagc gacttgtccc catgcagcag gtgaggttca ttgacatagc
     2641 ggtgccgccc ggccaccaga acattttctt ctgtatagcc caggttgcgc acatccgcaa
     2701 aaattttacc gatagtctgg caccagattt tctttgcggc cgtcgtgttc gtgcgcgctt
     2761 ccgcgccctt gcgaaaatgg tcataggtca cagggagacg gatgcgctgc tgtccaatgg
     2821 catttgggaa cgcaatcgcc acatccaggt caagtcttgg cgggccgccc ggacacggcg
     2881 caagcagaag aaatcctccg tgcggagcca caagagccac tggctccttc actgtgacat
     2941 aacgggcccg ggccgaagtg ttcgtgatac aggccttctc gagcgcctca accagatcca
     3001 ggctcccgcg attgaaaatc ggcggatccc cggaatccag caccacgcag agattgtcaa
     3061 ttcccagacc ggccttgagg gcaatgatat gctccaccat gcgcacataa ttgtggggcg
     3121 atccgcttct caggacgata ttgctgacca cctcacccgt cgtccagaca ttgcggatcg
     3181 acacctccgt cgggaggcag tctggcaggt cagacctccg aacccaccag ccttctttgt
     3241 cagtgggttc gaaacgtatc gtgcgcgtgg ctttcccgaa aaaggttccc gggccgcgga
     3301 cttccaccgg agtgctgatc gtccgctgga actcgccgaa cgggacataa acttcgccgc
     3361 tcaaatccaa atccaccggc tgcgccagaa actgctcgtg gttcttcctc agcagatcct
     3421 gatcgccgcc cagcagcatg ccgcaatcct gtatcgtcat acattgttct ccatctatgg
     3481 ctcaattccg cggcggcata ctggccgaag atccgagttc ggactgcatc gccttcttca
     3541 tgcagtccgg acacaccccg tgtgaaaact cggccagcgt tctgtcctga acgtaaaaat
     3601 caagccgttc ccatgcctgg tcattgagcc gtattttccc gcagtaagag cagaccacca
     3661 ggagcccctt cagcaccttc tgctcctgaa tataagtgtg ggcaatggtg atgaatccca
     3721 caacggctat ggccgccgtc agaat
//
LOCUS       DAOQWR010000187         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_25420_length_3741_cov_4.359197, whole genome shotgun sequence.
ACCESSION   DAOQWR010000187 DAOQWR010000000
VERSION     DAOQWR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25420_length_3741_cov_4.359197"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKK36_11210"
     CDS             <1..373
                     /locus_tag="PKK36_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95162.1"
                     /translation="NNEKYRPYQYKWSTTSSDWYALYDTAVNNYERLEGIILARPIFT
                     PYIVQANPDIESSRVVDQGDGTFTVEITVTNRPASAYTVEYIDSLTPVWNDTGASPAG
                     DTFSISVSNSIPKRFFRLIRN"
     gene            765..>3741
                     /gene="cas9"
                     /locus_tag="PKK36_11215"
     CDS             765..>3741
                     /gene="cas9"
                     /locus_tag="PKK36_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HNR95163.1"
                     /translation="MKQNKYTLGLDLGTNSIGWVLIEPGKSSKKHIVDLGVRCFEAGM
                     NINEATGKRDSHALERRMARGRRRNLSRRQMRKETTRKRLAEANLLPLGKPNHKDDTW
                     RGMLDLDPYSLRAKALDEKLERYELGRVFYHLAQRRGFLSNRKAGKSDKEEGVVKEGI
                     KALSAEMEESRARTLGEYFNMLDPHAQRIRTRYTHRQWYLDEFNAIWQAQKMHYGDVL
                     TDELHKKLKKAIFYQRPLKSVEKFIGHCALEKGRRRAPMACLAAQRLRYMQDLCHLRV
                     VSPEYESRPITADEFELLRRELEVSSRLTFSQARKRLGIKRGFKFNLEGGDRKDLKGN
                     TTVAKIMEAIGSRWLNLNSDEQNMMVEDLMSFTSEEALEKRARKFWKMAPEEAEAFSS
                     IDLEEGYSSFSRKAISKILPLLKPGCSIAEAVNEAYPDRGNAAAVDILPPLEDLRNPV
                     VQRTLAETRRLVNAIIAKHGKPARIHIELARDLKMPSKIREEQIRRMRAREKERKDAV
                     ARIIKEMDIVEPKRSAIEKVLLAEECGWECPYTGNPFKMSDLLGGQPTVDIEHIIPFS
                     RSFDNSFANKTLCDVHENREVKRNRTPWEAYGSTDKYDDILERVRRFTGAFAKVKLER
                     FSQKEVAAEDAFIEQYSSRQLNDTRYISSKAVEYLGQLYGAEWRKCITCVNGQTTAYF
                     RNAWELNKLLNTENLKNRDDHRHHAIDAMVVALTNQHNITALNTASKYSTDRGDFYEY
                     QPGRFRDMPEPWPGFLKEFSDALGRVIVSHRVSRRTNGQLHKDTVYGFIKSKEHNDEL
                     RPVVRTPLASLTEPDIRKKLIVDPVIRAKVEEVLAEKGQPPAKAFADRADHPVIVHPN
                     GQKQVVHKVRVYQSVTPVEISDVPPRHMALGNNHHLAIYAGKNRKGEEVWNCEVVPLL
                     EVKRRVASGKDAVRKTNERSDPLVFSLAIGDTVKMMWQGEEVIAVVQKLCHMPYPDYY
                     FKLHNDARMAKERKGCEIRV"
BASE COUNT         1015 a          910 c         1039 g          777 t
ORIGIN      
        1 caacaatgaa aaataccggc cctatcagta caaatggagc accacctcat ccgactggta
       61 cgcgctatac gacacggcgg tcaacaacta tgagcgcctg gagggcatca tcctcgcccg
      121 tcccatcttc accccctaca tcgtccaggc aaatccggac atcgaatcat cccgggttgt
      181 ggatcagggg gacgggacat ttaccgtgga aattacggta accaaccggc ccgcttcggc
      241 ctacactgtg gaatacatag actccctgac gcctgtctgg aacgatacgg gggcaagccc
      301 ggccggggac accttcagca tctccgtcag caacagcatc cccaaacgct tcttccgcct
      361 gatccgcaac tagcgccgcg gtcacaatac ttccaatgcc tggaagtttt tgtgaaaaaa
      421 gttccaatga ttggaacttt ttgcggcggt ttttccagtc attggaactt ttcgccgatt
      481 ttcctcctgc cggccgcgca gagattaaaa tagtactaat ttagtactat tttcagagga
      541 cgggaaatcg cattccagca gtccaaaccg ctgcagccgc gatgtataaa aaaagcgtcc
      601 cctgttcccg taaagagagc gcgaggacgc gagggcgcta attaaagcac tttttccttc
      661 cggatcgaaa tccttattgt gattaactgg acttcgccgt tcagatacat tacattgcac
      721 accatgatat ggaagtcctc tcaagtcaag aaggaaatca ccccatgaag caaaacaaat
      781 atacacttgg tttggatctc ggaacaaact ccataggatg ggtgcttatt gagccgggga
      841 aatcttcgaa aaagcacatc gttgatcttg gtgtccgctg ttttgaagca ggaatgaaca
      901 tcaatgaagc cacggggaaa cgcgattccc acgctcttga aagacgaatg gcgcggggaa
      961 ggcgcaggaa tttgtcgcgg aggcagatga ggaaggaaac cacgaggaag cggcttgcgg
     1021 aagcaaatct cctgcccttg ggaaagccaa accacaagga tgacacctgg cgcgggatgc
     1081 tggatcttga cccgtattcg ctgcgggcga aggctcttga tgagaagctg gaacggtatg
     1141 agcttggccg ggttttctac catctggccc agagacgcgg attcctcagc aaccgaaagg
     1201 ctggaaaaag cgacaaagaa gaaggcgtgg tcaaagaggg aatcaaagcg ctttcggcgg
     1261 aaatggaaga aagtcgtgcg cgcacacttg gtgaatattt caacatgctg gacccgcatg
     1321 cacaaagaat tcgaacgaga tatacgcatc ggcagtggta tctcgatgaa ttcaacgcca
     1381 tttggcaggc tcaaaagatg cattacggcg atgtgctgac agacgagctt cacaaaaaac
     1441 tgaagaaggc gatattttat cagcgtccac tgaaatcagt tgaaaaattc attggccact
     1501 gtgcactgga gaaaggccgc cggcgcgccc ccatggcctg tcttgcagcc cagcggcttc
     1561 gttacatgca ggatttgtgc catctgcggg ttgtgagtcc cgaatatgaa agccgtccga
     1621 ttactgcgga tgaatttgaa ctgctgcgcc gggaactcga agtcagcagc aggctgactt
     1681 tttcacaggc gaggaaacga ctggggatca agagagggtt caagttcaat ttggagggag
     1741 gcgaccggaa agatttaaag ggaaacacga cagtggcgaa aatcatggaa gccattggtt
     1801 cgcgctggct caacttgaac tcggacgaac agaacatgat ggttgaagat ctgatgagtt
     1861 ttacaagcga ggaggcgctg gaaaagcggg cgcggaaatt ctggaaaatg gcgccggagg
     1921 aggcggaggc cttcagctcg atagacctcg aggagggcta ttccagcttc tcccgaaaag
     1981 ccatttcaaa gatcctgccg cttttgaaac cgggctgttc tatcgccgaa gccgtgaatg
     2041 aggcatatcc cgacaggggg aatgcggctg cagtggatat tctgcctccg cttgaggact
     2101 tgagaaatcc tgtggtgcag cgcacactcg cggaaacgcg caggcttgtg aatgcgataa
     2161 tcgccaagca cgggaaaccg gcgcgcatcc atatcgaact ggcgcgtgat ttgaagatgc
     2221 cttcaaaaat ccgcgaggaa cagatcaggc gcatgcgggc gcgggaaaaa gaaagaaaag
     2281 atgccgttgc ccgcatcatc aaagaaatgg atatcgtgga gccgaaacga agtgcgatag
     2341 aaaaagttct gcttgcagag gaatgcggat gggaatgtcc ctataccgga aatcctttca
     2401 aaatgtccga tcttcttggc ggacagccga ctgtagatat tgagcacatc attcctttca
     2461 gccggtcgtt cgataacagt tttgcaaaca aaactctttg tgatgttcac gaaaaccgcg
     2521 aagtcaagcg gaaccggact ccgtgggaag cttatggatc cactgataaa tatgatgata
     2581 tccttgaacg tgtacgccgg ttcacgggtg cgtttgcgaa agtgaaactt gaaagatttt
     2641 cacagaaaga ggtggctgcg gaagacgcat tcatagagca atattccagc cgccagctga
     2701 acgacacgcg gtatatttcc agcaaggctg tggagtatct cgggcagctt tacggggcgg
     2761 agtggcggaa atgcattacc tgcgtgaacg gccagaccac ggcttatttt cgcaatgcat
     2821 gggagcttaa caagcttctg aatacggaaa atctgaaaaa cagggacgac catcggcacc
     2881 atgcgataga tgcgatggtg gttgcgctga caaatcagca caacatcacg gcattgaaca
     2941 cggcttcaaa atacagcacg gatcggggcg atttctacga ataccagcct ggccggttcc
     3001 gcgacatgcc cgaaccgtgg ccgggtttct tgaaggaatt cagtgacgct ctcgggcggg
     3061 tgattgtgtc gcacagggtt tcgcgccgca ccaacggcca gctgcacaag gatactgtct
     3121 atggattcat taaaagcaaa gaacacaacg atgaactgcg gcctgtggtg cgcactcctt
     3181 tggcgtcgct gaccgagccg gacatcagga aaaagctcat tgttgatccc gtcattcgcg
     3241 caaaggtgga ggaagtgctt gcagaaaaag gccagcctcc ggccaaggcc tttgctgatc
     3301 gggccgacca cccggtgatt gtacatccga atggacagaa gcaggttgta cacaaggtga
     3361 gggtttatca atccgttaca cccgtcgaga tttcagacgt tccgcccagg catatggcgc
     3421 ttgggaacaa tcatcatctg gcgatctatg cgggcaaaaa caggaaaggc gaggaggtgt
     3481 ggaactgcga ggtggttccc cttcttgaag tcaagcgccg cgtggcctcc ggcaaagacg
     3541 ccgtgcgcaa aaccaatgaa cggagcgatc cgcttgtgtt cagcctggcc ataggtgata
     3601 cggtgaaaat gatgtggcag ggggaagagg taattgctgt cgtacagaaa ctgtgtcata
     3661 tgccataccc cgattactat ttcaaattgc acaacgatgc ccgtatggca aaagagcgca
     3721 aaggttgtga aatcagagtg c
//
LOCUS       DAOQWR010000188         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_25455_length_3738_cov_12.022807, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000188 DAOQWR010000000
VERSION     DAOQWR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25455_length_3738_cov_12.022807"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PKK36_11220"
     CDS             complement(<1..1524)
                     /locus_tag="PKK36_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95164.1"
                     /translation="MLRKITAWLPIVLAAVCLQAGDSAVQSLVQFRFAAPETTEKAWV
                     SRDNSPPAVPGRGGGTTFRCPLRKRDRVYWDQTVSLDLSRFTSFEMDLACPHPEALRS
                     LGIYFKSGKGWYVWNKPPAYAGRQTVLMPRNLFSVEGSPAGWHKIEAIRFSPWKGNPE
                     NTEITVYSLAGRKDRILIMPGSETEPAEKAASRRAAQRLSDWLAGLNIPHGHLSGDAI
                     PAETRLLILPYNPALSAGQRKSVASFLQRGGKLMVFYSADPELARLMDVRLGAYAAAK
                     NRGQWSAFAFRNPNEWLVPARVPQQSWNIRPVTPLAGRGEIIAEWLDAEGRPTGDPAW
                     VSTGRGLWMTHILLGDNPQQKQAMLLGLLGRLEPEVWGRTAWMMLQRAGQIDSFRSLE
                     EAAAEITREAGKNRKIKQVEPLLEETMQHYRDALTLYEKNAFPAVTVACGRMRESLTR
                     AYSCAQAPVRKEFRGVWDQYAAGFHEKDWAGKCRLLAESGIRAVFPNVAKGGITSCRS
                     "
     gene            1753..2340
                     /locus_tag="PKK36_11225"
     CDS             1753..2340
                     /locus_tag="PKK36_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95165.1"
                     /translation="MKKTIAMIVLLCVAFTVPGVWAAPAKEQVTQGELAAILVNVLGL
                     ARFLPAAPTPYECAEVLALNGIQPKDGWNVDKVVTKADLARVIVLAMKRGDEVKNPDS
                     DKSWVDFLASLGVNIDSIGQAADSLRPLAEPVVANVFKGGSQTDPLKDSQEFGEPDSP
                     EFGTDANVALTYVALQQILQEVEFPPVVVRPMTPN"
     gene            2389..3585
                     /locus_tag="PKK36_11230"
     CDS             2389..3585
                     /locus_tag="PKK36_11230"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNR95166.1"
                     /translation="MNWKTCLCASIAVLFALQGIVLAQSEGRSYQLVNRLRLEFDDNI
                     REEIKGEEDESVKVIEEIEFIGNMSLEQTFISLRYKPGFVYWFDRDEDNEDIHHDLDF
                     ILNHEFTPRLSLGVKESLRIAELPELIERDTTIRQENDYTYNTVNGTLSVTLRPETRL
                     DIAGRHILLRYDEDEIADVQDYDILVGGLTLRNQFVPETAVLAELRAESIEYENTDVV
                     DRGSDTVQVGLGLEQMFSPALLGSARAGYMNKDMETESGSESSPYGEASLTFLPSPAT
                     RISLGASFALMETDIYPYSNQERTRFFASIAHDVTARISAYVSTAFTSSDYDADDIAS
                     GAEEDMLLLATSGSEDIVQVSARLTYQVTKSNWLETGWQYSDVDSELRGDYERNRIHV
                     GWKVQL"
BASE COUNT          755 a         1053 c         1134 g          796 t
ORIGIN      
        1 gctgcggcag gaggtgatgc cgcctttggc cacattgggg aaaactgcgc gtatcccgct
       61 ttcggcgagc agccggcatt tgcccgccca gtctttttcg tgaaatcccg cggcatactg
      121 atcccaaacc ccccggaact ctttccggac cggcgcctgt gcgcaggagt aggcccgggt
      181 gagactctcg cgcatgcgcc cgcaggcgac ggtgaccgcc gggaaggcat tcttttcata
      241 gagggtcagc gcgtcacgat aatgctgcat ggtttcttcc agcagcggtt caacctgttt
      301 gatcttcctg tttttcccgg cctcgcgcgt gatttccgcg gcggcctcct ccaggctgcg
      361 gaaggagtcg atctgtcccg cccgctgcag catcatccag gctgttcttc cccagacttc
      421 gggctccagc cttcccagca ggcccagcag catggcctgt ttctgctgcg ggttgtcacc
      481 gagcagaatg tgtgtcatcc agaggccccg gcccgtggag acccacgcgg gatccccggt
      541 cggccggcct tccgcatcca gccattcggc gatgatttct ccccggcccg cgagcggagt
      601 gaccggccgg atgttccacg actgctgcgg cacccgtgcc gggacaagcc attcattcgg
      661 attgcggaat gcgaaggcgc tccactgtcc gcggttcttt gcggcggcgt aggcgccgag
      721 cctgacgtcc atcaagcggg ccagttcagg atcggcggaa tagaacacca tcagttttcc
      781 gccgcgctga aggaatgaag cgactgattt ccgctgtccg gcggacagcg cggggttata
      841 gggcagaatc agcaggcgcg tttcggccgg gatggcatct ccggacaggt ggccatgagg
      901 gatgttgagt cctgccagcc agtcactgag gcgctgggct gcccgccggg aggcggcctt
      961 ttcggccggc tcggtttcgc ttccgggcat aatcaggatc cggtctttgc gcccggcgag
     1021 gctgtacacc gtgatctcgg tgttttccgg attccctttc cacggcgaaa aacggatggc
     1081 ttcgatcttg tgccagccag ccggagagcc ctcgacggag aagagattcc tgggcatcag
     1141 tacggtctgc cggccggcgt aggccggcgg cttgttccag acataccatc ctttcccgct
     1201 tttgaagtag atcccgagag accgcagggc ttcgggatgg ggacaggcca gatccatttc
     1261 gaagctggtg aatcgtgaca agtccagaga aacggtctga tcccagtaaa cccggtcccg
     1321 ctttctcagg ggacagcgaa aggttgttcc tccgcctcgc cccgggacgg cgggcgggct
     1381 gttgtcgcgc gaaacccatg ctttttcagt tgtttccggt gccgcaaaac ggaattgcac
     1441 cagcgactga acggcagaat ccccggcctg caggcagacg gcggcaagaa cgatcggaag
     1501 ccatgctgtg atctttcgca acatggcgcg gatactagca tgcagatgaa ctgaaatgaa
     1561 cgggtttttt cagggaatgt cagcctcccc ggcggccgtg agtgccgtaa aaaaaggttt
     1621 tttcatttga cggatacgcc tgcgcaaagg tagtttactg gaaattgagt caggttaacc
     1681 gctggaaata ttgtggcggg cggggatttt ttgaaaatac agaacaggat cagatcggag
     1741 ggtcggataa acatgaaaaa gacgattgcg atgattgtat tgctttgcgt ggcttttact
     1801 gtgccgggtg tgtgggcggc tcccgccaag gagcaggtga cccagggtga actggcggcg
     1861 attcttgtca atgttctggg tctggcccgg ttccttccgg ctgccccgac cccctatgag
     1921 tgcgccgaag tgctggccct caacggcatt cagcccaagg acgggtggaa tgtggacaag
     1981 gtggtcacca aggcggatct ggctcgcgtg attgtgctgg ccatgaagcg aggcgatgag
     2041 gtcaagaatc ccgacagcga caaatcgtgg gtggattttc ttgccagtct gggcgtgaat
     2101 attgacagca tcggccaggc ggcagacagt ctgcggcccc tcgcggagcc ggtcgtggcc
     2161 aatgtgttca agggcggttc acagactgac cccctgaagg acagtcagga attcggtgag
     2221 cccgactcgc ccgagttcgg caccgatgcc aatgtcgcgc ttacctatgt ggcgctgcag
     2281 cagattcttc aggaagttga atttccgccg gtggtcgtcc gtccgatgac ccccaactga
     2341 tattgattga ataaaatgat gatccgggaa acccaggaga ggaatattat gaactggaag
     2401 acatgtttgt gcgcgtcgat tgcggttctc tttgccctgc agggtattgt ccttgcgcag
     2461 agcgaaggcc gcagctatca gctcgtcaac cgtctgcgcc ttgaatttga cgacaacatc
     2521 cgcgaagaaa tcaagggaga ggaagacgaa agcgtaaagg tgattgaaga aatcgagttc
     2581 atcggaaaca tgagcctcga gcaaaccttc atcagtcttc gctacaagcc cggatttgtc
     2641 tactggtttg atcgcgatga ggacaatgag gacatccatc atgatttgga tttcatcctc
     2701 aatcatgaat tcacgccgcg tctgtccctt ggcgtcaagg aatcacttcg cattgcagaa
     2761 cttcccgagc tgattgagcg tgacacaacc atccgccagg agaacgatta cacctacaac
     2821 accgtgaacg gcaccctttc cgtgacgctt cgtccggaga cccgcctgga catcgccggc
     2881 cgccacatcc ttctgcgcta tgatgaggat gaaatcgcgg acgttcagga ctatgacatt
     2941 ctggtcggcg gtctcacgct tcgcaaccag ttcgttccgg aaacggccgt gctggccgaa
     3001 ctccgggcgg aatccattga gtatgagaat acggatgttg tggaccgcgg ctctgacacg
     3061 gttcaggttg gcctgggcct ggagcagatg ttcagcccgg ccctgctggg aagcgcccgc
     3121 gccggctaca tgaacaagga catggagacc gagtcgggaa gcgaatcgtc gccctatggc
     3181 gaagcctcgc tgaccttcct gccttcgccg gccacccgca tttcgctggg cgccagcttt
     3241 gcgctgatgg aaacggatat ctatccctac tccaatcagg agcgcacccg gttttttgcc
     3301 agcatcgcgc atgatgtcac ggcccgcatt tccgcctatg tctccacggc tttcacatcc
     3361 agtgactacg acgccgatga tatcgcctcc ggcgcggaag aggatatgct gctgctggcc
     3421 acgagcggtt cagaagacat tgtgcaggtc agcgcgcgcc tgacatatca ggtgacaaag
     3481 tccaactggc ttgaaaccgg ctggcagtac agcgatgtgg attcggaact gcgcggggat
     3541 tatgagcgca accggattca tgtcggctgg aaggttcagc tttaagatcg gtttgaaccc
     3601 ccgcccggcg ggggcgaatg catttatttg ctgaaggaag ctagtggggc gcactgtgcg
     3661 cctcactggc attttagaga cggaacagat atggaacagt cgaacgaagc acagctgcat
     3721 tttctggact actggcgg
//
LOCUS       DAOQWR010000189         3710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_25740_length_3710_cov_5.847880, whole genome shotgun sequence.
ACCESSION   DAOQWR010000189 DAOQWR010000000
VERSION     DAOQWR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25740_length_3710_cov_5.847880"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKK36_11235"
     CDS             complement(<1..872)
                     /locus_tag="PKK36_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR95167.1"
                     /translation="MKIIVVGGVAAGASAAARARRLDETAEIIILEQGRHVSFANCGL
                     PYHIGGDIRDREHLLLQTPESLKASLNLDVRTAHRVQSIDRAAKKIVVRDRDADQVYE
                     ETYDKLVLCPGASPIRPPLPGMDLPRVFVLRNVVDMDIIKREVDQGAKRAVVIGGGYI
                     GVEMAENLRHRRMDVELVELAKQIIPPLDPEMARDLEDHMRQQGVKLHLGVAASAFRQ
                     EGENLIVELASGKKIATDFVVLSIGVRPEAGLAKEAGLEIGPNGGIRVDEHMRTSDPD
                     IYAAGDAVEVIDTVT"
     gene            complement(891..1106)
                     /locus_tag="PKK36_11240"
     CDS             complement(891..1106)
                     /locus_tag="PKK36_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR95168.1"
                     /translation="MPLFEYKCRKCGHAFETLAASRSVKAKCPKCGSASVEKQLSTFS
                     TSSGSSLPCESGACSSGACPTGSCPFS"
     gene            complement(1110..1430)
                     /gene="trxA"
                     /locus_tag="PKK36_11245"
     CDS             complement(1110..1430)
                     /gene="trxA"
                     /locus_tag="PKK36_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR95169.1"
                     /translation="MSEKVKEIGKGQFEETIKTGVVLVDFWAPWCGPCRMQGPILETV
                     AEKVAGKAQIAKVNVDDLPEVAGQFGVRSIPTLIIFKNGQAAKTFVGVQQDAKLVSEI
                     EALV"
     gene            complement(1527..1697)
                     /locus_tag="PKK36_11250"
     CDS             complement(1527..1697)
                     /locus_tag="PKK36_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNR95170.1"
                     /translation="MPAAARIAIGAVAGALAGAAMYKFVGCRTGACPLTGNLYIAMFI
                     WALVGVMAAGVK"
     gene            complement(1700..2020)
                     /locus_tag="PKK36_11255"
     CDS             complement(1700..2020)
                     /locus_tag="PKK36_11255"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR95171.1"
                     /translation="MNTKPCSEGLSLAMFERVCEVLKVLAHPHRLKVIEVLQREESAP
                     VFEIAQRIGLPQAATSQHLNHLKRAGIVAARRTGKEVWYSIQDRRSISVLDCIREKGC
                     REGK"
     gene            complement(2113..2811)
                     /locus_tag="PKK36_11260"
     CDS             complement(2113..2811)
                     /locus_tag="PKK36_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNR95172.1"
                     /translation="MDSEKLKIDLEQNEDVIVAVCSGEIDMTTSDILNRKVGAQVRSR
                     NKKIVLDLSGVTFINSRGMGMLLEFNRQTKLGTGKFVLCGVPPAVMSSLQQLGIPSLI
                     RVFVTREDAVSALQKKGLSLDTRTEDGVLVVQATGAIDAFSVEAFDQAMRKHLEDQSN
                     SATLLNCSRIMYMNSRAIGALLDYHKASEKSGRKFALCEIDMRVLKVIQQLGIDALLL
                     IYPSEQEALQALKG"
     gene            3004..3654
                     /locus_tag="PKK36_11265"
     CDS             3004..3654
                     /locus_tag="PKK36_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95173.1"
                     /translation="MMVKYCGLWMLAVLMMLSLTACEDLYPFLYDDDDDDSGGAVAAA
                     PAGESTARKSTDRSAEQDSDDRDDSPPPADTSGSPGSVPADFAGVVWLHANVSGWAQT
                     ASLNAKVSGGNIVLNYDKAHSWPEVSAGGTRVVANPWVFVWENGKWYAATFEWLRKGQ
                     TSKPAGTVHGSHIKKAPLDDFVPQSGVRYGFMVSGLARDSTRSVQERSNVDMVTWP"
BASE COUNT          728 a         1154 c         1053 g          775 t
ORIGIN      
        1 gtcacggtgt caatcacctc cacggcgtcg ccggccgcat aaatgtccgg atcggaggtg
       61 cgcatgtgct catccaccct gatgccgccg ttggggccga tctccagacc ggcctctttc
      121 gccaggcccg cctcgggccg cacaccgatg gaaagcacga cgaaatcggt cgcgatcttt
      181 tttccgctgg cgagttcgac aatcaggttt tctccctcct ggcgaaaggc ggaagccgcc
      241 acgccgaggt gcagcttcac gccctgctgg cgcatgtggt cttcgagatc gcgggccatt
      301 tcaggatcca gcggaggaat gatctgcttc gcgagctcaa ccagttcgac atccatcctg
      361 cggtggcgca gattttcagc catttccacg ccgatataac cgccgccgat caccacggcc
      421 cgcttcgcgc cctgatccac ctcgcgtttg atgatgtcca tgtccaccac gttgcgcagc
      481 acgaacaccc ggggcaggtc catgccgggc aggggcgggc gaatggggct ggcccccggg
      541 cagagcacca gtttgtcgta ggtttcctca tacacctgat cggcgtcgcg gtcgcgcacg
      601 acgatttttt tcgcggcgcg gtcaatggac tgcacgcggt gagcggtccg cacatcaaga
      661 ttcagagagg ccttcaggct ttccggggtc tgcagcagca gatgctcgcg atccctgata
      721 tctccgccga tatggtacgg cagcccgcag ttggcaaacg agacatgccg cccctgctcc
      781 aggataatga tttcggctgt ttcatccagc cgccgcgcgc gcgcggcggc ggaggccccg
      841 gccgccactc cccccacgac aatgatcttc atctgaagtc ttccttccgg ttatgaaaac
      901 gggcagcttc ctgtcggaca cgcaccgctc gaacaggctc cggattcgca cgggagcgag
      961 ctgcccgagg aggtgctgaa cgtggaaagc tgtttctcga ccgatgcgga accgcacttc
     1021 gggcacttcg ccttcaccga acggcttgcc gcaagcgtct cgaacgcatg accgcatttt
     1081 ctgcatttgt actcaaacag cggcatggct caaaccagcg cctcgatttc cgaaaccagc
     1141 ttggcatcct gctgcacacc gacaaacgtt ttcgcggcct ggccgttctt aaagatgatc
     1201 agcgtcggga tgctgcgcac gccgaactgg ccggccactt cgggcagatc atccacgttc
     1261 accttcgcga tttgggcctt gccggcgacc ttttcggcca ccgtctcgag aatcggcccc
     1321 tgcatgcggc acggaccgca ccacggggcc cagaagtcca ccagcaccac gccggtcttt
     1381 atggtttctt caaactgacc ttttccgatt tctttcactt tctcgctcat ggctgtctcc
     1441 tgttttttcc aatggctgga acttttattt caaaaacttc caatgattgg aacttttcgc
     1501 gaaaaaagtt ccaatcattg gaaaaatcac ttcacgccgg cggccatcac gcccaccagc
     1561 gcccagatga acatggcaat atagagattg ccggtcagcg ggcaggcgcc ggtgcggcac
     1621 cccacaaact tgtacatggc cgcgcccgcg agggctccgg cgacggctcc gattgcgatt
     1681 cttgcggcag caggcataat cacttcccct ctctgcatcc cttttcacgg atgcaatcca
     1741 gaacactgat ggaacgccgg tcttgaatcg aataccagac ttcctttccc gtgcggcggg
     1801 cggccacgat gcccgcccgc ttgaggtggt tgagatgctg cgaggtggcg gcctgcggaa
     1861 gaccgatgcg ctgggcgatc tcaaacaccg gcgcgctttc ctcccgctga aggacctcaa
     1921 tgaccttgag ccggtgcggg tgcgccagca ccttgagcac ttcgcagacc cgctcaaaca
     1981 ttgcgaggga aagcccctcg ctgcatggtt tcgtattcat gacacaccaa tatattcata
     2041 tatcatgata tgtcaagata aaaaggaaaa aatccgcggg aagggtcgtt tttcaggcga
     2101 tttcggggcc gctcagccct tcagggcctg cagggcctcc tgctccgaag gatagatgag
     2161 cagcagcgca tcaatgccca gctgctggat caccttcagc acccgcatgt caatttcgca
     2221 gagcgcgaat ttccggccgc tcttctcgct ggccttgtga tagtccagca gcgcgccgat
     2281 cgcccggctg ttcatgtaca tgatgcgact gcagttgagc agcgttgcgc tgttcgactg
     2341 gtcctccaga tgcttgcgca tggcctggtc aaaagcctcc accgaaaatg cgtcaatcgc
     2401 tccggtggcc tgcaccacca gcaccccgtc ttccgtgcgc gtgtcgaggg acaatccctt
     2461 tttctgcagc gcgctgaccg catcttcgcg cgtgacgaag acgcgaatga gcgacggaat
     2521 gcccagctgc tgaagactgc tcataaccgc ggggggtacg ccgcagagga caaacttgcc
     2581 ggtgccgagc ttggtctgcc ggttgaattc cagcagcatg cccattccac ggctgttgat
     2641 gaaggtgact ccgctcagat caagaacgat tttcttgttg cggctccgca cctgagcgcc
     2701 gacttttctg ttaagaatgt ccgacgtggt catgtcaatt tcgccgctgc acacggccac
     2761 aatcacgtcc tcattctgtt ccagatcaat cttcagcttt tccgaatcca tggcacctct
     2821 ccattctgaa tagcccgact atagcggctt ttgaactctg cgcaatgcct ttctgcgcct
     2881 tcggcggaat aacatattcc ggaattttca gcattacggg gttgcaatat ccggaaaatt
     2941 taatattttc tggcgggacc ggtaacgaat gcggtgatgg attaacaata taacaggggc
     3001 ggaatgatgg tgaaatactg tggactctgg atgctggcgg ttctgatgat gctctcgctc
     3061 acggcttgtg aggatcttta tccctttctt tatgacgatg atgatgacga ctccggcggc
     3121 gccgtcgccg cggcgccggc gggcgagagt acggcccgga agtccacaga ccgctctgcc
     3181 gagcaggaca gcgatgaccg tgacgacagc ccgccgcccg ccgacacgag cgggtctccc
     3241 ggaagcgtgc ccgcggattt tgcgggagtg gtctggctgc acgcgaatgt gtcgggctgg
     3301 gcccagacgg ccagcctgaa tgccaaagtg agcggaggaa acattgtgct gaactacgac
     3361 aaggcccatt cctggcccga ggtcagcgcc ggaggaaccc gcgtggtggc caacccctgg
     3421 gtgtttgtct gggaaaacgg gaagtggtat gccgccacgt ttgaatggct gcgcaaagga
     3481 cagaccagca agccggcagg aacagttcac ggcagccaca tcaagaaggc gcccctggat
     3541 gattttgttc cgcagagcgg cgtccgctac ggcttcatgg tcagcggcct cgcgcgcgac
     3601 agcacacgca gcgtgcagga gcgcagcaac gtggatatgg tcacctggcc ctaggccccg
     3661 gccagcatct cgcgggcatg cttgagcgtg acgctggtca gctcgcggcc
//
LOCUS       DAOQWR010000190         3655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_26297_length_3655_cov_4.276111, whole genome shotgun sequence.
ACCESSION   DAOQWR010000190 DAOQWR010000000
VERSION     DAOQWR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26297_length_3655_cov_4.276111"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKK36_11270"
     CDS             complement(<1..737)
                     /locus_tag="PKK36_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95174.1"
                     /translation="MKNSFAAIIYLAAAVSISAVPVEINTTPYYSNDTACKVLANRLK
                     HVWRNITETSYEHVPTSVIDEMNGIYRFDCSGFICEILLRVALPDHYDDVYAYWETHK
                     TSIVGTNGLPMNTTRPLAGHFYDYFRDTILVSADNLSASNDYWYVFQDIRELQRGDLI
                     VVRYDDEWRQLYPNGTTGHMMIAWDIEALDEYNNSVVRVFDSTESPHTEAEDTRAQNN
                     PPNAIDQSGIGSGLMEFKASNNGKYRP"
     gene            1069..2628
                     /locus_tag="PKK36_11275"
     CDS             1069..2628
                     /locus_tag="PKK36_11275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNR95175.1"
                     /translation="MKKKVSESKSLESWIWDAACSIRGAKDAPKYKDYILPLIFAKRL
                     CDVFDDELNRMANQAGSRQKVFKLVKADHKLVRFYLPLMPDDPEEPVWSVIRKLSDKI
                     GEGVTTHMRAIARENPLLSGIIDRVDFNATTHGQRDLDDDRLSNLIEAISTKRLGLED
                     VEADIIGKSYEYLIRKFAEGGGQSAGEFYTPGEVAMIMAKVLDPEPGMEIYDPTCGSG
                     GLLIKCELAMEEKMSLRSRSKYAPLKLYGQEYVPETWAMANMNMIIHDMEGEIEIGDT
                     FKNPKFRKNGKLRTFDRVVANPMWNQDWFTETDYDSDELDRFPSGAGFPGKSSADWGW
                     VQHMHASLNGKGRAAVVLDTGAASRGSGNAGTNKEKTVRQWFVDNDLIESVLYLPENL
                     FYNTTAPGVVIFLNKAKPKERKGRIFLVNASQVFEKGDPKNFISEKGIEKIVRTLSGW
                     KEEEKLSRIVDHAELKKNDYNISPSRYIHTSDAETYRPIAEIVEELNAIEAEARETDK
                     ALTEILRKLGV"
     gene            2635..>3655
                     /locus_tag="PKK36_11280"
     CDS             2635..>3655
                     /locus_tag="PKK36_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNR95176.1"
                     /translation="MNTDQKLKSKIKVFVSSVQKELGPERAAVSQVIAVDPFLEKHCE
                     AILFENEPTPGRPQKKPYLDTLKECELYVLIVDVEYGGSDGKLSATHEEYREAQNRKM
                     PTAVFIRGLDGAKDRKREKSTQDFIDEIKSDEHKYKRFHDREDLKPAMREALLSILKN
                     QFQITPTKAEDSDSEHQIEKASGFELTAIEDLTVDRLDKAAVSDLIEAVIEPASRNLD
                     NAGEQALAMRGLAIRRPEKPSIVNRAACLLFHPHPATRFPQCEILADAYEEPRISGRP
                     KGQQTINAPLLEAVRQALAFIDKHSFHPRRVVGLNNVRLDEYPQKALREVLINRLAPV
                     SPELIK"
BASE COUNT          874 a          989 c         1019 g          773 t
ORIGIN      
        1 tagggccggt attttccatt gttgctggct ttgaactcca tgaggcccga tccaatgccc
       61 gactggtcaa tggcgttggg cggattgttt tgcgcgcggg tgtcttccgc ttcggtgtgg
      121 gggctttcgg ttgaatcaaa caccctgacc accgagttgt tgtattcatc aagcgcttca
      181 atgtcccatg caatcatcat gtgtccggtt gtgccgttgg gatagagctg tctccattcg
      241 tcatcatagc gcacgacgat caggtctccc ctctgaagtt cgcgaatatc ctgaaagaca
      301 taccagtagt catttgatgc ggagaggttg tcggcgctga cgaggatcgt gtcccggaag
      361 taatcataga aatgcccggc gaggggccgc gtggtgttca tgggcaggcc gtttgtgccg
      421 acgatggatg ttttatgtgt ttcccagtag gcatagacat cgtcataatg atccggcaat
      481 gccactctca gcaggatttc gcagatgaac ccgctgcagt cgaatctgta gatgccgttc
      541 atttcatcta tgactgaagt gggcacgtgc tcgtaggagg tctctgtgat attccgccag
      601 acatgcttga gccggttggc cagcactttg caggccgtat cattcgagta gtagggggtt
      661 gtgttgattt ccacagggac ggcgcttatt gagaccgctg cggccagata aatgattgcg
      721 gcaaaagagt ttttcatttt atggctcctg acattgttcg gcttcatctt ccacgtgttc
      781 aatttatcaa gtcaagccga tttgaccgca ggcggttcgt tattccgccg cgcgggagaa
      841 acagtccaat tattaacttt gtgttccggg gtcgtctgtt gttaacatgc tcacggatcc
      901 atgaatggag cgaaacgcaa aaagcggact ttaggggtct tgtccgcctt ttcgactgtg
      961 cttttcccag gcccgttctt ttcttttccc ggttgctatt ggtttgtccg aggcatagaa
     1021 tcacctccca gcgcatgcat tgtatcgctg aaggcaggat tgttaaggat gaaaaagaag
     1081 gtttctgaat caaaatcgct ggagtcgtgg atatgggatg cggcctgttc aatccggggt
     1141 gccaaagacg ctccgaagta caaggactat attctccccc ttatctttgc caagcggctc
     1201 tgcgatgttt ttgatgatga gctgaaccgc atggccaatc aggcgggatc acggcagaaa
     1261 gtcttcaaac tggtcaaggc cgatcacaag ctggttcgct tttatctgcc gctgatgccc
     1321 gacgatcctg aagagccggt ctggtcggtc atccgcaaac tctccgacaa gatcggcgaa
     1381 ggggtcacca cgcacatgcg ggcgattgcc cgcgagaatc cgctcctttc cggtattatt
     1441 gaccgcgtgg attttaacgc caccacccac gggcagcgcg atctcgacga cgaccggctt
     1501 tccaacctga ttgaggccat cagcaccaag cggctggggc tggaggatgt cgaggcggat
     1561 atcatcggca agagctatga atatctgatc cgcaagtttg ccgagggcgg gggacagagc
     1621 gcgggcgagt tctatacgcc cggcgaagtc gcaatgatca tggcgaaggt gcttgatccc
     1681 gagccgggca tggaaatcta tgatcccacc tgcggctccg gcggcctgct gatcaagtgc
     1741 gaactggcca tggaggagaa aatgagcctg cggagccgga gcaaatatgc cccgctcaaa
     1801 ctctacgggc aggaatacgt ccccgaaacg tgggccatgg ccaacatgaa catgatcatc
     1861 cacgacatgg aaggcgaaat cgaaatcggt gacaccttca agaatcccaa gttccgcaag
     1921 aacggaaaac tgcgcacgtt tgaccgggtg gtggccaatc cgatgtggaa tcaggactgg
     1981 tttacggaga cggactacga cagcgatgaa ctcgaccgct ttccgtccgg cgccggattc
     2041 cccggcaaat cgtccgccga ctggggctgg gtgcagcaca tgcatgccag tctgaacggg
     2101 aagggcaggg cggcggtcgt gctggacacc ggcgcggcct cgcgcggatc gggaaatgca
     2161 ggcaccaaca aggaaaagac cgtccgccag tggttcgtgg acaacgatct cattgagagt
     2221 gtgctttatc tgcccgaaaa ccttttctac aacaccaccg ctccgggtgt ggtgatcttt
     2281 ctgaacaaag ccaagccgaa ggaacggaaa ggcagaatat ttctggtcaa cgccagccag
     2341 gtcttcgaaa agggcgatcc caaaaacttc atttccgaaa agggcatcga aaaaattgtc
     2401 aggacgctgt ccggctggaa agaggaggaa aaactcagcc gcattgtgga ccatgcggag
     2461 ctgaagaaaa acgactataa catctccccc agccgctaca tccacaccag cgacgccgaa
     2521 acctaccggc ccatcgccga aatcgtcgaa gaactgaacg ccatcgaagc agaagcgcgc
     2581 gagacggaca aggccttaac cgaaatattg aggaaactcg gagtctaaca acggatgaac
     2641 acggatcaaa agctcaagtc gaagataaaa gtctttgtca gctcagttca gaaagaactc
     2701 gggccggaac gagcggcggt ctcccaggtt attgccgttg acccgtttct cgaaaagcat
     2761 tgcgaggcca tactattcga gaacgagccc acgcccggcc gcccccaaaa aaagccctat
     2821 ctcgacacac tgaaggaatg cgaactctat gttttgatcg tggatgttga atatggcgga
     2881 tccgacggca aactttccgc cacgcacgag gaataccggg aggcacagaa tcgaaagatg
     2941 cccaccgccg tctttattcg cggtctcgac ggcgcgaagg acaggaaacg ggagaaatcc
     3001 actcaggatt tcattgatga gatcaaatcc gacgagcata aatacaagcg cttccatgac
     3061 cgcgaagact tgaagcccgc gatgcgggag gcgctcctct caatcctcaa gaaccagttc
     3121 cagatcaccc cgaccaaggc cgaggacagc gactcggagc accaaatcga gaaagcgtct
     3181 ggtttcgaac tcacagcgat tgaagacctg accgttgatc gcctcgacaa agcggctgta
     3241 tcggatctca tcgaagcggt gatcgaacca gcgtcccgga atcttgataa tgcgggagaa
     3301 caggcgcttg ctatgcgggg tcttgccatc cggcgcccgg agaagccttc cattgtcaac
     3361 cgtgcggctt gtctcctgtt tcacccgcat ccggccacgc ggttccccca atgtgagatc
     3421 ctcgccgatg cctatgagga accgagaatt tcaggtcgtc ccaaaggcca gcagacgatc
     3481 aacgcgccac tgctcgaagc cgtccgacaa gcgctggcct tcatcgacaa acacagcttc
     3541 cacccccggc gcgtagttgg tctcaacaac gtgcggctcg acgagtatcc ccagaaggcg
     3601 ttgcgcgaag tgctgatcaa ccgcctggcg ccggtcagcc cggagctgat caagt
//
LOCUS       DAOQWR010000191         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_26359_length_3650_cov_3.142698, whole genome shotgun sequence.
ACCESSION   DAOQWR010000191 DAOQWR010000000
VERSION     DAOQWR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26359_length_3650_cov_3.142698"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..2963
                     /locus_tag="PKK36_11285"
     CDS             90..2963
                     /locus_tag="PKK36_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNR95177.1"
                     /translation="MTTVTQEGTNTTTESEWEAIDFDELGSFGSASQVLPVGTWENSL
                     SAITLQDTTTVTRSYNGYVGLRFQSMNINLNCETVEGAALEFNVVQGPVADLTFDLYG
                     EVNYYGGSQRFGEGNAALPSQRIPDTAIMQVTIPAGFTGVTNIDISTVFNELVTKKRP
                     LGDTIGLIFDASTAAEAAFIKDSETWLIVAYIPMYKIPAEWLAGGTVQTCPDIVPSQR
                     KSHGMVYDYLRDRMVLFGGMNGMQVFDETWEGEPRFAFRNGTQNMLSPRMVAWSKVET
                     DFAPPARWGHSMVYDDVNERTLIFGGFDKNNEPLNDLWAYYGSKEIEITVTNITGTNE
                     IGEVQTETETIPASWVKITDFKDSQRPSPRGGAMMVFFGGAFYDRGFEDYSYGHRDKV
                     VLFGGTDGKSYFNDLWVYDENFSDPCLDSCGDAGGRWILVDPGGEHSQGPSPRAFGSF
                     VFAQNGALSPDAEGLGTYGKDAAGRKRSAKSCAYLFGGRTGTLPTSKDTDQDMVDDGT
                     EYELGGPAAGRDPRVNALVQKDMGEMMPFAFNRIGTDMGGYSPPLRGAIANLEALSYV
                     DRVHAFRMGALLSTAYFPFQGYPLETCLDYKQAEISDNWTDQPYDDNNTNMLLWIIGV
                     DAISPDWTNLWYHRAGFEDPDDSMDVWALGVPNNSSLGPIAAPPYAHSGRWCYGTGLT
                     GPYPNDAVMELYSPIVNLNVPAVNGTSTNNMNSYFLVFYEWLHLADSNDYVKIDAVRP
                     ETDADVRTRKPGLNKPVITVLGRRSNSSNTRGSWRKVVVPLDSLGGEPQVYFRFVLQS
                     DSKNTSGGWYIDDIAILQGAELYGNYGSMAGEEVELLGENYNNNVQAVTQTGEGGYFE
                     FGLLPTGNYMIDGVPAAITPDAAVVEIAVLMFDGIGPAAGGREISWMTETGWTYWLEY
                     TTDFVTWNPLHTEGPVVVPGVFPFTDPSGDPLRAYRILGSPPP"
     gene            3048..>3650
                     /locus_tag="PKK36_11290"
     CDS             3048..>3650
                     /locus_tag="PKK36_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95178.1"
                     /translation="MKKYLVWLCIASLSVVLAGCDWSAGGSSDGFNTSKGAGVNINVS
                     GVYSGAINGRAVERTSGAGQILSLVVSQSGNTVEVTDNQGSRYQGTIGAPGAVATATG
                     GIYPAGAELVQYQINFSGKDNVAAQDVEFVGTIHAVSVSDIRGTSSTSGSSTTDQNTR
                     TTTETVVDVVKDTTTDTITTTIGAPGDPFYRVTTQVIVTDN"
BASE COUNT          817 a          921 c         1076 g          836 t
ORIGIN      
        1 ctattccctc tatatattat aagtctacag atgaccctcg tcctgtcact gagagactcg
       61 ttgttgcaca ggatgttgct gaagctttag tgacgacggt gactcaagaa gggacaaaca
      121 caactacaga atctgaatgg gaagcaattg atttcgatga gttgggctca ttcggaagcg
      181 ccagtcaggt attgcctgtt ggtacttggg aaaattcgct ttctgcaatt actttgcagg
      241 acacgacaac tgtaacacgt tcgtataacg gttatgttgg gcttcgtttt caatcaatga
      301 atattaattt gaactgcgag actgtcgaag gagctgcact tgagtttaat gtagtgcagg
      361 gtccggttgc tgatctcact tttgatctgt atggcgaagt caattactat ggagggtcac
      421 agcgttttgg agaagggaat gcggctctgc cctcccagag aataccggac accgcaatta
      481 tgcaggtaac aattcctgct gggtttacag gcgtaacaaa cattgacata agtacagtat
      541 tcaatgagtt ggtaacaaag aaacgtcctc ttggtgacac tatcggcctg atttttgatg
      601 cgtcaacggc cgcagaagca gcatttatta aggattccga aacatggcta attgtagcct
      661 acatacccat gtataaaatc ccggctgaat ggttggctgg aggcacggtg cagacttgtc
      721 ctgacattgt gccaagccag aggaagtcgc atgggatggt ttatgattat ctgagagacc
      781 gtatggtgtt gtttggcggc atgaacggta tgcaggtgtt tgatgaaact tgggaggggg
      841 agccacgttt tgcattccgg aatggaacgc agaatatgct gtcgccgcga atggtggctt
      901 ggtctaaagt tgaaacagac tttgctccgc ccgcccgctg gggtcatagc atggtttatg
      961 atgacgtgaa tgaacgcacc ctgatttttg gcggatttga taaaaacaat gaaccactga
     1021 atgatctttg ggcttattat ggatccaagg agattgaaat aaccgttact aatattacag
     1081 gtaccaatga aatcggtgag gttcagactg agactgaaac tattccagcg agctgggtca
     1141 agattactga tttcaaagac agtcaacgcc cttccccgcg cggcggcgct atgatggtat
     1201 tcttcggcgg tgctttttat gaccgtggat ttgaagacta tagctatggc cacagggaca
     1261 aagtcgttct gtttggtgga accgatggga agagttattt caatgacctt tgggtctatg
     1321 atgagaactt ctccgaccct tgcttagact cctgcgggga tgctggtggc cgctggatac
     1381 tggtggatcc cggcggcgag catagtcagg gcccgtcgcc ccgcgcgttt ggatcgtttg
     1441 tgtttgccca gaacggcgct ctgtcgccgg atgcagaagg tttgggtacc tatggcaaag
     1501 atgccgctgg gcggaagcgc agcgccaagt cctgtgccta tctgttcggc gggaggaccg
     1561 gcactcttcc gacctcgaag gacacggatc aggatatggt ggatgacggc accgagtatg
     1621 aactgggcgg cccggccgcc ggccgcgacc cgcgtgtgaa cgccctggtc cagaaggata
     1681 tgggcgagat gatgccgttt gcgttcaacc gcatcggcac ggacatgggc ggctactctc
     1741 cgcctctgcg tggggcaatc gcgaatctgg aagcactatc ctatgtggac cgcgtccatg
     1801 ccttccgaat gggagctctg ctgtccactg cgtatttccc gttccagggc tatccgctgg
     1861 aaacatgcct cgactataaa caggcggaaa tctctgacaa ctggacagat cagccgtatg
     1921 atgacaacaa taccaatatg ctgctgtgga ttataggtgt tgatgcaatt tcgccggact
     1981 ggaccaacct ctggtatcac cgggcgggat ttgaggatcc ggacgattcg atggatgtct
     2041 gggcgctggg tgtgccgaac aattccagcc tggggccgat tgcagcgccg ccgtatgcgc
     2101 acagcggccg ctggtgctac ggcaccggcc tgaccggccc gtatccgaat gatgcggtga
     2161 tggagttgta ctcgccgatt gtgaacctga atgttccggc ggtgaacggc acctccacca
     2221 acaacatgaa cagctacttc ctggtgttct atgagtggct gcatctggct gactcgaacg
     2281 actatgtgaa gattgatgcg gtccggcccg aaacggatgc cgatgtccgc acgcgcaaac
     2341 ccggcctcaa taagccggtg atcacggtgc tgggacgtcg cagcaattcc tccaacaccc
     2401 gcggaagctg gcggaaagtg gtggtcccgc tggacagtct tggcggcgag cctcaggttt
     2461 atttccgctt tgttcttcag tctgacagca agaacacctc cgggggctgg tacattgatg
     2521 atatcgcgat tctgcagggc gccgagctct atggaaacta tggttcaatg gccggcgagg
     2581 aagtggaact gctgggcgaa aactacaaca acaatgtcca ggctgtgact caaaccggcg
     2641 aaggcggtta tttcgagttc ggcctgctcc cgaccggaaa ctatatgatc gacggcgttc
     2701 ccgccgccat cacgccggat gcggcggtgg tggaaatcgc cgtgctgatg ttcgacggaa
     2761 tcggtccggc cgcaggcggg cgggaaatct cctggatgac cgaaaccggc tggacctact
     2821 ggctggaata caccacggac ttcgtgacct ggaatcctct gcatacagag ggccctgtcg
     2881 tggtcccggg tgtgttccca ttcacggatc cgtcgggtga tcctctccgg gcgtaccgga
     2941 ttctcggcag cccgcctcca tgatgagcgg tgcgattagg gtttgacggg acaggggccc
     3001 gtgattagga tgttgcgaaa tttgaatgaa caggtgaagg agtcagcatg aagaaatatc
     3061 tggtttggtt gtgcatcgcg tcgctctcgg ttgtgcttgc cggctgcgac tggtcggccg
     3121 gaggaagttc cgacggtttt aatacctcaa aaggcgccgg ggtgaatatc aatgtgtctg
     3181 gtgtttattc cggagccatc aacggccgtg cggttgaaag aacgtccggc gcgggacaga
     3241 tcctgtcgct cgtcgtcagt cagtccggca acaccgttga ggtgaccgac aaccagggct
     3301 cccgctatca gggcaccatt ggcgcgccgg gcgcggtggc aacggccacg ggcggcatct
     3361 atcccgcggg ggctgaactg gtccagtacc agatcaactt ctccggaaaa gacaacgtgg
     3421 cggcgcagga tgtggaattc gtgggcacca ttcacgccgt ttccgtgtcc gatattcggg
     3481 gaacaagctc caccagcgga agctccacca cggatcagaa cacccgcacc accaccgaga
     3541 cggtcgtgga tgtcgtgaaa gacaccacca ccgacaccat caccaccacg atcggagccc
     3601 ccggggatcc cttctaccgc gtgaccacgc aggtaatcgt gacagacaac
//
LOCUS       DAOQWR010000192         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_26519_length_3635_cov_4.571229, whole genome shotgun sequence.
ACCESSION   DAOQWR010000192 DAOQWR010000000
VERSION     DAOQWR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26519_length_3635_cov_4.571229"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PKK36_11295"
     CDS             <1..1112
                     /locus_tag="PKK36_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR95179.1"
                     /translation="IALQGRGRIQIGDGPQTDSKWDLITERMGLDAVRKLYAEQKQFE
                     IELIDLRDEHWIEQDGIYTDRIKLKGDPAGKSLFDLGANSMFAEFDGRDKIYYGAYYD
                     VDETNRHHSGGKHEYEVSNSPLAADVFINIPKLKTHKKCGLTVNLKSLVGINANKNCL
                     PHYIIGSPETGGDQFDKLRARGRLENTLVLSVKRRLLKNSRAVQTAARRLKKLAYRIF
                     GGTEEVVRSGNWHGNDTVWRMCLDLNRILLYGRPGGKFVGDPKKFFSLVDGIVAMEGN
                     GPVAGDSRPAGLIIAGDNPVAVDAVCARLMGFDENKLPLVRRAFDAHELPLMDGDITV
                     VSSEGKWSGDYRKWQKNELLNFRPHFGWTGHVELE"
     gene            1109..2509
                     /locus_tag="PKK36_11300"
     CDS             1109..2509
                     /locus_tag="PKK36_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95180.1"
                     /translation="MSYGTGIRQRLYNASPSLIKDALATVYGLQQRHARYGDDFVNHL
                     ALLLQLQYWPREALLAYQREQLRIFLRNTVSATPYYASRPEYAEAAESGDISNLPLLP
                     KSVVKNNPAEFVNGDLKNIPHRMVHTSGTTGQALVFPMANSCFQREYAYKQIHYSWGK
                     VSLLNRQPFAFCAGHPVAGPGRRRPPFWTRDSANNHLFMSSYHLSDENLPHYIRELER
                     FQPAMLGGYPSSIYLLALAAQKAGGCRVRPKAVYTFSETLLDYQRALIEEIFGCRVFN
                     WYGNTEICANIVECECGELHMKLEHSYVEVLNDENKPCSPGETGRLVCTGFGNPAFPL
                     VRYEIGDVVTLSGNQQAACGRGGILVDKVLGRVEDYIVTPDGRLVGRLDHLFKDSVNV
                     AEAQICQQDRNEVVLKIVKKDLYGDRDEKAVLREARTRLGKEIGIKFEYVDSIPRAAG
                     GKFRFIHSTINRKEIA"
     gene            2506..3600
                     /locus_tag="PKK36_11305"
     CDS             2506..3600
                     /locus_tag="PKK36_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95181.1"
                     /translation="MKRFLGKIFQGNGRMSAAVILLLYLLIFAPFFLMVAGDAQVHLA
                     VAERFVDGHPFTYNADEIVVASTSPFWTILLVALMHMAGPAAVLLLKVLVTVIYALVS
                     AALCAAAGKYFRVEAPFIKLLLGLWLLCIPVLANAVSGLENILSALQVLLVLLLSERF
                     FDPDRRGVGCSTAMGLVLGWALLTRPDGGALCLLAVALLHLFRLVQLKKWKTEIISMG
                     VCVFFAALVLLPWYAYQYRITGKVFTDSSLARMYAGRRASVMIIPDVLYFHPKALVTL
                     LSFFAPISLGAVIWAASLCRSFFAERADWRAHLHRHLVEYASLVIIAAGLVFYSFVVG
                     ADHFGRYFLVIFPLFFVAGICGLNTLLSQY"
BASE COUNT          736 a          970 c         1102 g          827 t
ORIGIN      
        1 acatcgcgct ccagggccgg ggccgcatcc agatcggcga cgggccgcag accgactcga
       61 aatgggatct catcacggag cgcatgggcc tggatgccgt ccgcaaactc tacgcagagc
      121 agaagcagtt tgaaatcgag ctgattgatc tgcgcgacga gcactggatc gagcaggacg
      181 gaatttacac cgaccgcata aaactgaaag gcgatccggc cgggaaatcc ctttttgatc
      241 tgggtgcgaa cagcatgttt gcggagtttg acggccggga caagatatac tacggagcgt
      301 attacgacgt ggatgagacc aaccgccatc acagcggggg gaagcacgaa tatgaggtct
      361 ccaattcgcc gctggcggcg gatgtgttca tcaacattcc caagctgaag acgcacaaga
      421 agtgcggatt gacggtaaac ttgaaaagcc ttgtgggcat taatgccaac aagaactgtc
      481 tccctcatta tatcatcgga tcacccgaga ccggcggcga ccagttcgac aagctgcgtg
      541 cgcgcggccg gctggagaat accctggtgc tttcggtgaa aaggaggctg ctgaaaaaca
      601 gtcgcgcggt gcagactgcc gcccgtcgcc tgaagaagct ggcctacaga atattcggcg
      661 gaacggagga ggtggtccgc tcgggcaact ggcacggcaa tgacaccgtg tggcggatgt
      721 gccttgatct gaaccgcatt ctgctttacg gccgccccgg cggaaagttt gtcggggacc
      781 ccaagaaatt cttctcgctg gtggacggga tcgtggccat ggagggcaac ggtcctgtgg
      841 cgggtgattc ccgtcccgcg ggcctgatta ttgccggcga caatccggtg gcggtggatg
      901 ccgtctgcgc ccggctgatg gggtttgatg aaaacaagct gccccttgtg aggcgggcct
      961 ttgatgcaca tgaacttccg ctgatggacg gcgatattac cgttgtcagc agcgagggga
     1021 aatggtccgg cgattacagg aagtggcaga agaacgaact gctgaatttc cgcccgcact
     1081 tcgggtggac cggacatgtg gagctggaat gagctacgga accggaatca ggcagcggct
     1141 gtacaacgcg tcgccttcgc ttatcaaaga tgccctggcg acagtctatg ggttgcagca
     1201 gcgccacgcg cgttatggcg atgattttgt aaaccatctt gccctgctgc ttcagcttca
     1261 gtactggccg cgcgaggccc tgctggccta ccagcgcgag cagctgcgca tttttctcag
     1321 aaataccgta tccgccacgc cctattatgc atcacgtccc gaatacgcgg aggcggcgga
     1381 gagcggtgac atctcgaacc tgccgctgct tccgaaatcg gttgtgaaaa acaatccggc
     1441 cgaatttgta aacggtgact tgaagaacat tccgcacaga atggtgcata ccagcggcac
     1501 gaccgggcag gcgctggtgt ttcccatggc taattcctgc tttcagcgcg aatatgccta
     1561 caagcagatt cactattcat gggggaaggt aagcctgctg aaccggcagc cgtttgcatt
     1621 ttgcgccgga cacccggtgg cgggtcccgg ccggcgccgt cccccgttct ggactcgcga
     1681 cagcgcaaac aatcacctgt ttatgtcgtc ctatcatctg tccgatgaaa accttccgca
     1741 ttacatccgg gagctggaac gctttcagcc cgcgatgctc gggggctacc cgtcctcgat
     1801 ttatctgctt gcgctggccg cgcagaaggc gggcggctgc cgggtgcggc cgaaggcggt
     1861 ttacacgttc tctgaaaccc tgctggatta tcagcgcgca ctgattgaag aaatattcgg
     1921 gtgcagggtc ttcaactggt acggcaacac ggaaatctgc gccaatattg tggaatgcga
     1981 atgcggtgaa ctgcacatga agctggagca cagctatgtc gaagtgctca atgacgagaa
     2041 caagccttgt tcgccgggcg aaaccggacg gctggtctgc accgggttcg gcaatccggc
     2101 gtttccgctg gttcgctatg aaatcggcga tgtcgtgact ttgtccggaa accagcaggc
     2161 cgcctgcggc cgcggaggga ttcttgtgga taaggttctg ggccgggtcg aggattacat
     2221 cgtgacgccc gacggccgtc ttgtcggccg cctggatcat ctgttcaagg acagcgtcaa
     2281 tgtggccgag gcgcagatat gccagcaaga cagaaacgaa gttgtcctga aaatcgtgaa
     2341 aaaggatttg tacggggatc gggatgaaaa agccgtcctg cgcgaggcgc gaacgaggct
     2401 tgggaaagaa atcggaatca aattcgaata tgtggattcc attccgcgcg cggccggcgg
     2461 aaagttcagg ttcatccact caaccatcaa caggaaagaa atcgcatgaa gcgttttttg
     2521 ggaaaaatct ttcaggggaa cgggcgcatg tctgcggccg tcatcctgct cctgtacctg
     2581 ctcatctttg cccctttctt cctgatggtt gcaggagatg cccaggttca tctggcggtg
     2641 gcggaacgct ttgtggatgg gcatccgttc acttacaatg cggacgaaat cgtggtggca
     2701 tccaccagcc ctttctggac gatcctgctg gtggcgctga tgcacatggc cgggccggcc
     2761 gccgttctgc tgctgaaggt gctggtcacg gtcatctatg ccctggtatc ggcggcgctt
     2821 tgcgccgcgg ccgggaaata tttccgtgtt gaagcgcctt tcatcaaact gctgctgggc
     2881 ctgtggctgc tctgcattcc tgtgctggcc aacgcggtgt cgggactgga aaatatactg
     2941 tccgcactgc aggtgcttct tgtcctgctg ctctcggagc gcttcttcga tccggatcga
     3001 cggggtgtcg gctgcagcac ggcaatgggg ctggtgctcg ggtgggcact tttgacgcgg
     3061 cccgacggcg gggcgctctg cctgcttgcg gtggctctgc tgcatctttt ccgactggtg
     3121 cagttgaaga aatggaaaac ggaaatcatc tccatgggtg tatgtgtgtt ctttgccgcg
     3181 cttgttcttc ttccgtggta tgcctatcag tatagaatta ccggaaaagt tttcacggat
     3241 tcctctctgg cgcgaatgta tgccggaaga agagcttcgg tcatgatcat ccccgacgtg
     3301 ctctattttc atccgaaggc tttggtaacg ctgctgtcct tttttgctcc gatctcgctt
     3361 ggggccgtaa tatgggcggc ctctctgtgc cgctcctttt ttgcagagag agccgattgg
     3421 cgtgcacatc ttcaccgcca tttggtcgaa tatgcatccc tggtgatcat tgcggccggc
     3481 ctggtcttct attcttttgt ggtcggcgca gatcattttg gaagatactt cctcgtgata
     3541 ttcccgctgt tctttgtggc gggaatttgc gggctgaaca cacttctgtc ccagtattaa
     3601 ttccacagca tcaactggtt aggcggtgat agtgt
//
LOCUS       DAOQWR010000193         3620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_26681_length_3620_cov_5.411501, whole genome shotgun sequence.
ACCESSION   DAOQWR010000193 DAOQWR010000000
VERSION     DAOQWR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26681_length_3620_cov_5.411501"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKK36_11310"
     CDS             <1..354
                     /locus_tag="PKK36_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNR95182.1"
                     /translation="GAGTVNFAGKTNIAEWAALMQLCRVVVCNDSGGMHLAAAAGVPV
                     AAVFGITDPRKTGPLGRHVRILQNSGIQQRDISRHSEPAVEALKAISPEQVFGEVQHL
                     LQETDKTKRDTDHAR"
     gene            344..1156
                     /locus_tag="PKK36_11315"
     CDS             344..1156
                     /locus_tag="PKK36_11315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR95183.1"
                     /translation="MRDKISACITCMNEEDNIRRCLESVRWCDEIVVVDSFSTDRTVD
                     ICKEYTERVYQHEWLGYIGQKNLIRGMASHPWVLFLDADEEVSPGLRDEILRELARPD
                     NSYAGYRFPRKVYYLYKWICHGEWYPDTKLRVFRKDKGHSEGREPHDLVIVDGPVKTL
                     INPLHHYTYSNIRDHLASVGNFSVITAEEKFKEGTRFSYADWLVRPPWRFFKAYFLKL
                     GFLDGRRGFFIAAISAFAVLIKYAFLWEKELDENHRSAATAEDGAGNPEGQD"
     gene            complement(1147..1527)
                     /locus_tag="PKK36_11320"
     CDS             complement(1147..1527)
                     /locus_tag="PKK36_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95184.1"
                     /translation="MKLTPAQRNEIFRKVRAILVRYWIDLGFISIHVSEQTIYIRGSL
                     KRLAGVPEQLSGAVVEAMFAEIKGLRGINHVQAQFDNWSGSVSAGGWNPRDAVAVSNW
                     ERMRMASAGKPSTFEAEEVSDPQS"
     gene            complement(1524..1928)
                     /locus_tag="PKK36_11325"
     CDS             complement(1524..1928)
                     /locus_tag="PKK36_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR95185.1"
                     /translation="MRKILSVDDDDAILRCFQRALDGRGYKVFVTTDPAEVSKILEEH
                     ELDLVMLDIHMPKKDGFEIFQELKRQKKKLPVLFVTAYPKSFSMEDQKVLELWQNEFA
                     DGNTDVLYKPFKLERLFEKVEGLIGPPSEHGE"
     gene            complement(2010..3047)
                     /locus_tag="PKK36_11330"
     CDS             complement(2010..3047)
                     /locus_tag="PKK36_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNR95186.1"
                     /translation="MKDVFTDLEKWHHAGLKTAVALLVNAQHSSPRLPGARMAVNENG
                     DMAGSVSMGCVEGDLREHLLKTIRDGAPRLIHYGISEDLAASVGLSCGGEIDVLLHVY
                     DDAVIPLDAWGCIAEGGRGVYFCGLEGKALGRELLLLADGSRHGSLGSETADKEAADI
                     ARDLFRTGGTLRYQMQGTGEKVFAESSQPAAPLAVIGASPLSAAVCKLAAFADYDVTL
                     IDPRKAFAVAENYPDVSRIIHQWPDDGLKAAGVDEQWYIAVLAHDAKLDVPALAYALK
                     AKCRYVGLLGSRRTQESRKQALREIGIAERDIRRIHGPIGVEIEAETLREIAVSVVAE
                     LIAVRRGKIHD"
     gene            complement(3047..>3620)
                     /locus_tag="PKK36_11335"
     CDS             complement(3047..>3620)
                     /locus_tag="PKK36_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNR95187.1"
                     /translation="LGLAPAAKNHLVDFGSIICDCKTGISGAGKKLQAAFHYPARYDH
                     MNAYKLGGHQHVCEVERELSGQAGSAIKVTFTAQVVPMCRGIMSCLYGTLGPGQNAAT
                     VAEAYREFYKDSPFVRICDRNSGVGTVHVRGTNFCNLIVDVDERTSRLRVVSHIDNLM
                     KGQAGNALQNMNLIFGLPETMGLDRPGQYP"
BASE COUNT          818 a         1106 c          952 g          744 t
ORIGIN      
        1 ggggcgggaa cagttaattt cgccggaaaa acaaatattg cagaatgggc ggcgctcatg
       61 cagctttgcc gggtcgtggt ctgcaacgac agcggaggaa tgcatctagc cgccgcggcg
      121 ggcgttccag tggcggcggt attcgggatc acggaccccc gcaaaaccgg tcctctgggc
      181 cgtcatgtcc gcatactgca gaacagcgga attcaacagc gcgatatcag ccgccactcc
      241 gagccggccg tcgaagccct gaaagccatc tcgcccgaac aggtgttcgg cgaagtccag
      301 catctgctgc aggagacgga taagacaaaa agggacactg atcatgcgag ataaaatttc
      361 agcctgcatc acctgcatga atgaagagga caacatccgg cgctgcctgg aaagcgtgcg
      421 ctggtgtgat gaaatcgttg ttgttgacag cttcagcaca gaccgcactg tggatatctg
      481 caaggaatat accgaacggg tctatcagca cgaatggctg ggctatatcg ggcagaagaa
      541 cctgattcgc gggatggcgt cccacccatg ggttctgttt ctcgatgcgg acgaagaagt
      601 gtccccgggc cttcgggatg aaatcctcag ggagctggct cggccggaca attcatatgc
      661 tggataccgt tttccgcgga aggtatacta tctctacaaa tggatctgcc acggggaatg
      721 gtatccggac accaaactga gggtcttccg gaaggacaag ggacacagtg aaggacgcga
      781 accgcatgat cttgtcattg ttgacggccc cgtgaaaacg ctgatcaacc ccctgcatca
      841 ttacacctac agcaacatcc gcgatcatct ggcttccgtt ggaaatttct ctgtcataac
      901 cgccgaggaa aagttcaagg aaggcacccg cttcagctac gcggactggc tggtgcggcc
      961 gccgtggcgt ttcttcaagg cctatttcct gaaactcgga tttctggacg gacggagggg
     1021 cttctttatc gcagccatca gtgcttttgc tgttctgatc aaatacgcgt ttctctggga
     1081 aaaagagctg gacgaaaatc accggagtgc agcgacagcc gaagatggcg caggaaaccc
     1141 cgaaggtcag gactgagggt cagaaacttc ctctgcctca aaggtggaag gctttcctgc
     1201 tgatgccatt cgcatgcgct cccagttgga aacggccacg gcatcccgag ggttccatcc
     1261 cccggcactc acgcttccgc tccagttgtc aaactgggcc tgcacatggt taatgccccg
     1321 aagccccttg atttcagcga acatggcctc cacaacagcc ccggacagct gttccggcac
     1381 acccgccagc cgtttcagag aaccacggat ataaatcgtc tgctcggaca cgtgaatgga
     1441 aataaaacca agatcaatcc agtagcggac taaaatggcc cgcactttcc tgaaaatctc
     1501 gttccgctgg gcaggagtta atttcattcc ccgtgttcag agggaggccc gatcaggccc
     1561 tccacctttt caaaaagacg ttcgagctta aaaggtttgt agaggacatc cgtgttgccg
     1621 tcggcaaact cattctgcca gagttccaga accttctggt cctccatcga aaatgacttg
     1681 ggataagccg tcacaaacag caccggcagt ttcttcttct gccgcttgag ttcctgaaat
     1741 atctcgaagc cgtccttctt gggcatgtga atatccagca tgaccagatc cagttcatgt
     1801 tcctcaagaa tcttcgacac ctccgccggg tcagtggtca caaagacttt gtatccccgg
     1861 ccgtccaaag cacgctgaaa gcagcgcaga atcgcatcgt catcatccac ggacagaatt
     1921 tttctcacgg ctatctcctc ttctgaaaca cacaccatct gcggtcattc tacttccccg
     1981 gcaaagagcc gtcaatgacc gcgccgccat caatcgtgga ttttgccccg gcgcacggca
     2041 atcagctcag ccaccaccga cacggcgatc tcccgcagcg tctcggcttc aatctcaacc
     2101 ccgatcggcc cgtgaattct ccggatgtcc cgctctgcaa tgccaatctc ccggagcgcc
     2161 tgtttgcggc tttcctgcgt gcggcgactg cccagcagcc cgacatagcg gcacttcgcc
     2221 ttcaaggcat aagccagcgc gggcacgtcg agtttcgcgt catgcgccaa aacggcaata
     2281 taccactgct catccacacc ggcagccttc aggccgtcat caggccactg gtgaatgatg
     2341 cgcgacacat cgggatagtt ctccgccact gcaaaggcct tgcggggatc aatcagcgtg
     2401 acatcatagt cagcaaaagc cgccagttta caaaccgccg ccgacagcgg cgaggcgcct
     2461 ataaccgcca ggggagctgc cggctgggag gattccgcaa atactttttc accggttccc
     2521 tgcatttgat accgcagagt tcccccggtc ctgaaaaggt cgcgggcaat gtccgcagct
     2581 tctttatctg ccgtctcgga acccagcgat ccgtgccggc ttccgtccgc cagcagcagc
     2641 agctcgcgcc caagggcctt tccctccaga ccgcagaaat atacccccct cccgccttcg
     2701 gctatgcagc cccaggcgtc caggggaatt acagcatcat catacacatg gagcagaaca
     2761 tcaatttctc cgccgcatga caggcccacg ctggccgcca gatcttccga gataccatag
     2821 tgaatgagac ggggagcgcc atcgcgaatt gttttcagaa gatgttcgcg aagatcgccc
     2881 tccacacacc ccatcgagac agaaccggcc atatcgccat tttcattcac ggccatgcgc
     2941 gcgcccggca ggcgcggcga ggaatgctgc gcgttcacca gcagggccac agccgtcttg
     3001 agtccggcat gatgccattt ctccagatct gtgaagacat ccttcatcag gggtattgtc
     3061 cggggcgatc aaggcccatg gtttcgggca ggccgaaaat cagattcata ttctgcaggg
     3121 cattgcccgc ctgccccttc atcagattgt cgatatggga aaccacgcgc aggcggctgg
     3181 tccgttcatc cacatccaca atcaggttgc agaaatttgt tccgcgcaca tgcaccgtgc
     3241 ccacgccgct gttccggtcg cagatacgca caaatgggct gtccttgtag aattcccggt
     3301 aggcttcggc cacggtggcg gcgttctgcc ccggaccaag agtgccatac aggcaggaca
     3361 taatgccgcg gcacattgga accacctgcg cggtgaacgt caccttaatg gcgcttcccg
     3421 cctgcccgct gagttcccgc tccacctcac aaacatgctg atgcccgccc agtttgtagg
     3481 cattcatatg gtcatagcgg gccggataat gaaatgccgc ctgcagtttt ttgccggcgc
     3541 ctgaaatgcc ggtcttgcag tcgcagatga tgctgccgaa atccaccaga tggttcttcg
     3601 ccgcgggagc cagtcccagg
//
LOCUS       DAOQWR010000194         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_27608_length_3533_cov_4.366590, whole genome shotgun sequence.
ACCESSION   DAOQWR010000194 DAOQWR010000000
VERSION     DAOQWR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27608_length_3533_cov_4.366590"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKK36_11340"
     CDS             <1..948
                     /locus_tag="PKK36_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95188.1"
                     /translation="DVIVSIKAYANTLPSALRVKKRRGSAVIVYLDEWDGAVYYQLSR
                     SRRLGAWMREWHHPMSEIYTPLVEKMIPSADAVLSTTQFLRDKFGGYVIRMGVDCKKF
                     CPQPEERVARLRKSLGLEGCRLIVFGGVARPHKGIELLLDAICRVHSCKIRLLVAGPR
                     TPWLERLMTNPEYGHVLRCAGDALDAASVINQQVHDRLPEYLDLADVIVLPQRDTLLA
                     RSQMPIKVFEAMAMAKPIIASNVSDLPVVLEGCGRIVPPDDPAALAEAVEHVLNHPEE
                     AAAMGLAARRKCVNMYSREQTEKEILEIFRKHDRRAGRA"
     gene            945..2288
                     /locus_tag="PKK36_11345"
     CDS             945..2288
                     /locus_tag="PKK36_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95189.1"
                     /translation="MNSYCSAGLSRILIDRRDGFRMKVLKAADRLQAADRLSRDFIHA
                     QQGAALAALLEHARDTVPAWRDRIKGPEHIEGADAYHVLQDIPVLSKEELKENMARFL
                     SSVSGGAVDNATGGSSGIPLRFKVDRMRQIAGEAAHIWGNSMTGWKYGDRLAMLWGSD
                     QDVREALADARRALRCWIENIRWYNAFDMGEDRMADYHRAMTRSRPHLIVAYAGSALA
                     YADYLQRRRITPHYPLKGIVTSAEVLTDAMRSTIETVFQKPVFDRYGSREFGPVAAEC
                     PERSGMHINEMDCVLEIQSDDPCSIPGPILITYLRNYAMPFIRYDTGDLGVFMPEEEC
                     ACGRQTRRLARVIGRQSDIITTASGRQIHGEWFTHLMYGLEGIRQFQLIQETRFQYRL
                     VLVADWHTASRHENMLREKILNAVGPESIVNFEYVQHIYPMPSGKHRFTVSMVGR"
     gene            2652..3494
                     /locus_tag="PKK36_11350"
     CDS             2652..3494
                     /locus_tag="PKK36_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNR95190.1"
                     /translation="MTVHNNMEDVLLFLHIPKAAGTTLTSVLMRQYPPESIFEVYGLV
                     PDRRIEELKTCSPDRRRIVQCVIGHFYFGLDELFDRRCRYITMLRDPVERVISSYYHV
                     LRTPDNWLYRDVAKSGMTLEQYVKSEGPIAHEARNMQTRMMAGTAWDCILDEASLKTA
                     KENLDKCAAVGVVERFDESLVLFKRRLGWTGLRYLKMRAGGRPRNSDISPSVLDAIRT
                     HNELDILLYQYAVQRLQMDIAGEGRGFVVDLMRLRTANRIYSVYSSLRRKLQPSRGRK
                     ACNL"
BASE COUNT          820 a          887 c         1021 g          805 t
ORIGIN      
        1 gatgtcatcg tctccatcaa ggcatatgca aatactctgc cgtcggctct ccgcgtgaaa
       61 aagagacgcg gcagcgcggt cattgtgtat ttggatgaat gggatggagc cgtctattat
      121 cagttgagca gaagcagacg tttaggggcc tggatgagag aatggcatca tcccatgagt
      181 gagatataca caccgcttgt ggaaaagatg attccctctg cagatgcggt gctttccacc
      241 actcagtttc tccgggacaa gtttggcgga tatgtgatcc gaatgggggt cgactgcaaa
      301 aagttctgtc ctcaaccgga agaacgcgtg gcccggctca ggaagtccct tggcctggag
      361 ggctgtcgcc tcatcgtgtt tggcggtgtg gcccggccgc ataaggggat tgagctcctg
      421 ctggatgcaa tctgccgtgt acacagctgc aaaatacgat tgcttgtggc gggcccgcga
      481 accccatggc ttgaacgttt gatgacgaac ccggaatacg gccatgtgct gcgctgtgca
      541 ggagatgcgc ttgatgccgc ttctgtcatc aatcagcagg tccatgaccg gctcccggaa
      601 tatcttgatc ttgcagatgt gattgtcctg ccgcagcggg acacacttct ggcgaggtcg
      661 caaatgccga tcaaggtttt tgaggccatg gccatggcca agccgattat tgcatcaaac
      721 gtttctgatc ttccggttgt gctggagggc tgcggcagga ttgtgccgcc ggatgatccc
      781 gccgcactgg ccgaagccgt tgaacatgtg ctgaatcatc cggaagaggc cgcggctatg
      841 ggcctggccg cccgcagaaa atgtgtgaac atgtacagca gagagcaaac cgaaaaagag
      901 attctggaaa tattccggaa acacgacagg agggcgggca gagcatgaat tcgtattgtt
      961 cagccggatt gtcccgcatc ctgatcgacc ggcgcgatgg cttccgcatg aaggttctta
     1021 aggcggccga ccggttgcag gcggccgacc gcctctcacg cgattttatt cacgctcagc
     1081 agggagctgc tcttgccgcg ctgctggagc acgcgcggga tacagttccg gcgtggagag
     1141 acagaattaa agggcccgaa cacattgaag gggctgacgc ataccatgtg cttcaggata
     1201 tacccgtgtt gtccaaggag gagctgaagg aaaacatggc tcgctttctg tcctcggttt
     1261 cgggtggcgc tgttgacaat gcaaccggcg gttcttccgg cattccgttg agattcaagg
     1321 tggaccgcat gcggcagatt gcgggggagg ctgcgcatat ctggggaaac agcatgacgg
     1381 ggtggaagta cggtgatcgc ctggccatgc tctggggatc ggatcaggat gtgagggagg
     1441 ctcttgcgga tgcccgtcgc gcgctgcgct gctggattga aaatatccgc tggtacaatg
     1501 cctttgacat gggcgaagat cgaatggctg attaccaccg ggcgatgacc cggtcccgcc
     1561 cgcacctgat tgtggcttat gccggttcgg ccctcgccta tgcggactat ttacaacgcc
     1621 gccggattac tccgcattat ccgttgaagg ggatcgtcac ttcggcggaa gtactgaccg
     1681 atgccatgcg aagcaccatc gagacggtgt ttcaaaaacc cgttttcgat cggtacggaa
     1741 gccgggagtt cggcccggtg gcggccgaat gccctgagcg aagcggcatg cacatcaatg
     1801 aaatggactg cgtactggaa atacagagcg atgatccctg cagcatcccc ggcccgattc
     1861 tgatcactta tctcagaaac tacgccatgc cgtttatacg ctatgacacg ggcgatctgg
     1921 gggtgttcat gcccgaagag gaatgcgcct gcggcaggca aacccggcgt ttggcccgag
     1981 tgatcgggcg gcagtcggat attattacaa cggcatcggg cagacaaatc cacggtgagt
     2041 ggttcaccca tctcatgtat ggacttgagg gcatccggca gttccagctg attcaggaga
     2101 cacggtttca ataccggctg gttctggtgg ctgactggca tacagccagt cggcacgaaa
     2161 atatgctgcg ggaaaagatt ctgaacgctg tgggtcctga gagcattgtg aattttgagt
     2221 atgtccagca tatttatcct atgccttcgg gcaagcaccg gtttacagtg tcgatggtcg
     2281 gcagatgagg ggcaacggcg caaagtggca agggcacaat gtggagcatg caatggggac
     2341 cacgggcttg aggcttttga ctaaaaagcc ttgaagatac gaacagtatg aagaagaaac
     2401 cacggattac ccagcgcggc agggctgcaa ccaaagatgg aaccacgaat gaacgcgaat
     2461 caacacgaat gaagaaagca acgaatattt aaatagctat ctcccactta tcatggaact
     2521 tgtaacccgt agccttaatc ttgccaccgt ttccaagtct ccagtctcaa gtcttttctt
     2581 caacctgcaa cttgtaacta tttccaaagt ctctagtctc tagtctgtct ttaatctgca
     2641 actctatggc catgacagtt cataataata tggaagatgt cctgttgttt ctgcatattc
     2701 caaaagcagc agggaccaca ctgaccagtg tgcttatgcg gcagtatccc ccggagagca
     2761 tttttgaagt atatggactg gtcccggatc gacgcataga agaactgaaa acctgttccc
     2821 cggaccgccg ccgcattgtg cagtgtgtga ttggtcattt ctatttcggc ctggacgaac
     2881 tgtttgaccg gcgctgtcgc tacattacca tgcttcgcga tcccgtcgaa agggtaatct
     2941 cgtcatacta tcatgttctg cgaactccgg acaactggct gtatcgggat gtggcgaaga
     3001 gcggaatgac gctggagcaa tatgtaaagt ctgaaggccc cattgcccat gaagcccgaa
     3061 atatgcaaac ccgcatgatg gcggggaccg cctgggattg catcctggat gaagcatccc
     3121 tcaaaacggc taaagagaat ctggacaaat gtgctgcggt gggggtggtg gaaagatttg
     3181 atgaatccct tgtgctgttc aagcgccggc tgggctggac cggactccga tatcttaaaa
     3241 tgcgggcggg cgggcgcccc cggaacagcg atatttctcc atccgtatta gatgcaattc
     3301 gaactcataa tgaattggat attctgttat atcagtatgc tgtgcagcgg ctgcaaatgg
     3361 atattgccgg agaaggccgg gggtttgtgg ttgatttaat gcgtcttcgc actgcaaacc
     3421 gaatctattc cgtctattcc tccctccgca ggaagctgca accatccaga ggccggaaag
     3481 cctgcaacct ttaatcttta actttgaacc atccagaggc tggcaagcct gca
//
LOCUS       DAOQWR010000195         3516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_27780_length_3516_cov_4.724935, whole genome shotgun sequence.
ACCESSION   DAOQWR010000195 DAOQWR010000000
VERSION     DAOQWR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27780_length_3516_cov_4.724935"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKK36_11355"
     CDS             complement(<1..467)
                     /locus_tag="PKK36_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR95191.1"
                     /translation="MNILITGANGFIGRRLVRRLADDGHSVIAVDRMASRKDLPAGAR
                     HVVSDFADASTALCVEKKPFALAHLAWEMDRSRDQSGQAERFGRFLEECAARGLVRVV
                     ALGSSEEFGSRGGVIRDEDEPVPPVTPYGAAKRAARSTLQLFSEKHNIPALWL"
     gene            complement(522..920)
                     /locus_tag="PKK36_11360"
     CDS             complement(522..920)
                     /locus_tag="PKK36_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR95192.1"
                     /translation="MNGDYVNGEKTPIKTYRDLHVWQDGVVFVTELYRLTRDFPREEM
                     FGVVSQIRRAAISIPSNIAEGYGRRSSRDYVRFLQISMGSLFEIQTQLEIARNLEYLS
                     SDTYESLYAMSRKIERMLSSLIRKIQERKE"
     gene            complement(1024..1827)
                     /locus_tag="PKK36_11365"
     CDS             complement(1024..1827)
                     /locus_tag="PKK36_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNR95193.1"
                     /translation="MINQKTDPKHIPVVILAGGRGTRLMEETQTIPKPMVSIGGKPML
                     LHIMRYYSAYGFRKFVLCLGYKGHIIKDYFLDIHKHTASLIVNGKTGEYKYHTETAQD
                     WEIQLIETGEHSLTGTRLQKAGAHIDAPHFCLTYGDGLCDVDLGRELEFHLKHGKTGT
                     VAAVHPPSRFGRLDIAEDGRVQSFREKETLTHDFINGGYFIFKREFLDRLPASGNYSL
                     ESEPLARLAEDGKLCAYRHEGFWMCMDTMRDRETLEKIYESGKAPWARA"
     gene            complement(1931..2818)
                     /gene="rfbA"
                     /locus_tag="PKK36_11370"
     CDS             complement(1931..2818)
                     /gene="rfbA"
                     /locus_tag="PKK36_11370"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016718975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNR95194.1"
                     /translation="MKGIILSGGSGTRLHPLTKVISKQILPVYDKPMIYYPLSTLMLA
                     GIRDILLISTPHDLPLFKQMLGDGSHLGLKMSYVEQPSPDGLPQAFILGEKFIGKDNV
                     AMILGDNIFYGHNFAGMLKEAAERKTGSTIFAYPVRDPERYGVVEYDASGRAVSLEEK
                     PAEPKSKHAVTGLYFFDNRVVEISKSLKPSARGELEILDTIRPYMKSGELHVQELGRG
                     FAWLDTGTFESLLEAGTFVETIERRQGLKISCVEEIAWRKGFITTDQLRALGESMKKN
                     GYGAYLLAIAEEERDKGTK"
     gene            complement(2926..>3516)
                     /locus_tag="PKK36_11375"
     CDS             complement(2926..>3516)
                     /locus_tag="PKK36_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HNR95195.1"
                     /translation="LVVSAYRRSFFSKSDIRVATARAGNVIGPGDWAANRIVPDCIRS
                     LAAGEPIRVRNPQSVRPWQHVLEPLSGYLLLGAQLQQTPEWADAWNFGPESEAARTVG
                     ELVDRVISCWGSGACVLPEIKNAVHEAGLLTLNIDKAEKNLDWHPVWNFDQTVSHTVD
                     GYQDMLKCTGDPEAIRAMMAREIAAYDKDQRLKTDM"
BASE COUNT          783 a         1061 c          859 g          813 t
ORIGIN      
        1 cttaaccaca gagcggggat attgtgtttt tcagaaaaca gctgaagcgt actccgggcc
       61 gcgcgtttgg ccgcgccata gggagtcacc ggaggaaccg gctcatcctc atcacgtatc
      121 acgccgcctc gggaaccgaa ctcctccgaa gatcccagcg cgacaacccg gacaagaccc
      181 ctcgccgcac actcctccag aaaccgccca aagcgctctg cctgaccgct ctgatccctt
      241 gaccggtcca tttcccacgc cagatgcgcc agcgcaaaag gttttttctc gacacacagc
      301 gccgtcgacg catcggcaaa atctgaaaca acatgccggg cacctgcggg caagtccttc
      361 cgagatgcca tccgatcgac cgcaatcaca gaatgcccat catcggccag tcgccgaacc
      421 agccgccggc cgataaatcc atttgctcct gtaataagaa tattcatgac ggcgagagtg
      481 acaaaggcac aggggcacaa gggggcatgg tttcagaggc cctattcttt tctttcttga
      541 atctttctga taagcgagct cagcatacgt tcaatctttc tgctcatcgc atataaagac
      601 tcataggtat cggagctcaa atattcaagg tttcgtgcta tttccaactg cgtctgaatt
      661 tcaaaaagag aacccatgga tatctgcagg aatcgaacgt aatcccgtga tgaccggcgg
      721 ccatatccct ctgcgatatt gctgggaatc gaaattgccg cacgtctaat ttgtgatacg
      781 actccaaaca tttcctctct cgggaaatct ctcgtcagtc gatacagctc agtaacaaag
      841 acaacgccat cctgccatac atgcaaatcc ctgtatgtct ttatcggagt cttctcgcca
      901 ttgacgtaat ccccattcat cctatttgcc gctccaaata ccgcaaccct ctgccccctg
      961 cttctctgtg cccttgcccc cttgaacccc tgtccctttg tgcccttgtg cccgcccccc
     1021 gtgctacgcg cgggcccagg gggccttgcc gctttcgtag attttttcca gagtctcccg
     1081 gtcgcgcatg gtgtccatgc acatccagaa tccttcatgc cgataggcgc agagtttgcc
     1141 atcctccgcc agccgggcaa ggggttcaga ttcaaggctg tagttgcctg aagcaggcag
     1201 gcgatccaaa aattccctct taaagatgaa atacccgcca ttgatgaaat cgtgcgtcag
     1261 cgtttctttt tcgcggaacg actgcacccg tccgtcttcc gcgatatcca gccggccgaa
     1321 gcgcgagggc ggatgaaccg ccgcgacagt tcccgtcttc ccatgcttta aatgaaactc
     1381 cagctccctt cccagatcca catcgcacag cccgtcaccg taggtcaggc agaagtgagg
     1441 cgcatcaata tgcgcccccg ccttctgaag ccgtgttccg gtcaggctgt gctcgcccgt
     1501 ttcaatgagc tgaatttccc agtcctgagc cgtttcggtg tgatatttgt attcaccggt
     1561 cttcccgttc acaatcaggc tggctgtatg cttatggata tcgagaaaat aatccttgat
     1621 aatatgcccc ttgtagccga ggcagagaac gaatttccgg aacccataag cgctgtaata
     1681 ccgcatgata tgcagcagca tgggcttccc gcctatagac accatgggct tggggatagt
     1741 ctgcgtttcc tccatcagcc gcgtgccgcg gccgccggcg aggatgacaa cgggtatatg
     1801 ctttgggtct gtcttttgat ttatcattat ttttcttcct tcataatatt ttcgtgcaga
     1861 ggcactgaag agtgggcaca aaggatcaag ggcacacggg cacaaggaga atcaaatcct
     1921 tgcccctctg ctactttgtg cctttgtccc tctcttcctc cgcaatcgcc agcaaatagg
     1981 ccccatagcc attctttttc atggattcac ccagcgcgcg aagctgatcg gtggttataa
     2041 aacctttgcg ccatgcaatt tcctctacac aggatatttt cagtccctgc cggcgctcaa
     2101 tggtctccac aaaggttccc gcctccagca gcgattcaaa ggtcccggtg tccagccatg
     2161 cgaaaccgcg gcccagctcc tgcacatgaa gctctccgct tttcatatac gggcgaatcg
     2221 tgtccaggat ttccagttcg ccgcgggcgg agggcttgag acttttcgat atttccacga
     2281 ctctgttatc gaagaagtac aatcccgtga ccgcatgctt cgatttcggt tcggcgggct
     2341 tttcctccag cgataccgct ctcccgctcg catcgtactc gaccactccg tagcgttcag
     2401 ggtcgcgcac gggataagca aatatcgtcg agccggtttt tctttccgcg gcttccttca
     2461 gcataccggc gaagttatga ccgtaaaata tgttgtcgcc cagaatcatg gccacattgt
     2521 ccttgccgat gaacttttcg cccagaatga atgcctgcgg cagaccgtcc gggcttggct
     2581 gctcaacata ggacatcttc aggcccagat gagagccgtc cccaagcatc tgcttgaaca
     2641 ggggcaggtc atgcggtgtg ctgatcagca gaatgtcccg aatccccgcc agcataagcg
     2701 tggagagcgg ataatagatc atcggcttgt cataaaccgg aaggatctgc ttgctgatga
     2761 ctttcgtaag cggatgcaga cgcgtgcccg aaccgccgga taggataatg cctttcataa
     2821 gacctcagaa gttaaaggtt gaaggttaca ggttgcaggt ttaaagaaag actgaagacc
     2881 agagactaca gactagagac tttgaagagg gagtgttctt tcacatcaca tatccgtttt
     2941 cagtctctgg tctttatcgt acgccgcaat ctcgcgcgcc atcatggccc ggatggcctc
     3001 cggatcaccc gtgcatttca gcatgtcctg ataaccgtca acggtatgtg aaaccgtctg
     3061 gtcgaaattc cagaccggat gccagtccag attcttttcg gccttgtcga tattaagcgt
     3121 caggagcccc gcctcatgaa ctgcattttt gatttcaggc aacacacacg caccgctccc
     3181 ccagcaggat atcacgcggt ccaccagctc gcccaccgtg cgggcggcct cagactcggg
     3241 gccgaagttc catgcatcgg cccattccgg cgtctgctgc agctgcgccc ccagcaggag
     3301 ataacctgac agcggctcca gcacatgctg ccagggccga acggactgag gattacgaac
     3361 ccgaatcggt tcgcccgcgg ccagagaccg gatgcagtcg ggcacaatgc ggttggccgc
     3421 ccagtcgccc gggccgatca cattgccggc gcgggcggtt gccacgcgga tatccgattt
     3481 tgaaaagaat gaacggcgat atgccgaaac cacaag
//
LOCUS       DAOQWR010000196         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_28074_length_3491_cov_5.146391, whole genome shotgun sequence.
ACCESSION   DAOQWR010000196 DAOQWR010000000
VERSION     DAOQWR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28074_length_3491_cov_5.146391"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="PKK36_11380"
     CDS             <1..1912
                     /locus_tag="PKK36_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR95196.1"
                     /translation="KVEDLRDQLVANNETIHWVPGYVGSKRFGNWLQDAKDWNISRNR
                     FWGSCLPIWISEDGADMICVGSIAELEKLSGEKVNDLHKHIVDQITFVKDGKTYRRTP
                     EVLDCWFESGSMPYAQQHYPFERKDELKKFFPAHFIAEGLDQTRGWFYTLLILSTAIF
                     KKSCYRNVVVNGLILAEDGRKMSKRLKNYPDPVHILNTYGADALRLYMIHSPVVRAED
                     LRFSEEGVKDALRHLLIPLWNAYSFFVTYARVDGWKPSDSNGRALAGNAHLLDRWILS
                     SLDHLISEVTGAMDVYDLQQAVRPFVAFIEDLTNWYIRRSRRRFWKSTDDEDKAMAYR
                     TLYTVLLQLSKVAAPFIPFISETIYRNLRTDDMPESVHLCDFPQSSGENRDESLEKQM
                     AAVMSVVVLGRQLRSAHDLKVRQPLPAIHVISRDASLLEQIQELKDIIAEELNVKNIW
                     FSGHEADLVTLKAKPNFRNLGPRLGPAVKQAAAAIQKISQHELESVIEGRGTAAVEIA
                     GQKLELGLTDLIIERIPHPDMVVASERELVVALETGLSEELVREGLAREFVNRVQNMR
                     RTMDMDVTQRIRLRFTGDEALREALDEHGEYIRSETLALDLVYAGETDEGSEAADLNG
                     HACSIKVEIVKS"
     gene            2009..2758
                     /locus_tag="PKK36_11385"
     CDS             2009..2758
                     /locus_tag="PKK36_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNR95197.1"
                     /translation="MSAKKAKKAVKKKAVSAKKPAKKAAVKKAARKPAARKPVKKSVA
                     KKPVRKSAVKKPVKKVARKAAKKPAKKAAVKKAARKPAAKKPAAKKAAASKPAKKPEV
                     KKPLVTVKVKFTAKQIKIFRQRLHELRDRVVDGINFLAGDNLTRSPRDSAGDLSNYSL
                     HMADQGTDNFDREFALSQLSGEQDVIYEIDEALQRLNEGSYGVCEDCGCAIGQERLLA
                     LPFARRCIKCKAIYEKVHTKYRPLGPPVDMK"
     gene            2773..3231
                     /gene="lspA"
                     /locus_tag="PKK36_11390"
     CDS             2773..3231
                     /gene="lspA"
                     /locus_tag="PKK36_11390"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNR95198.1"
                     /translation="MLALLIALFITAADQITKYIIRTEYVLAESRTVIPGFMDLTYLR
                     NTGAAWGMLGGQNAFLIIFSVVMLALMILFRKAFLSSTLDHRIALGLMAGGILGNLLD
                     RLRLGWVTDFLDFYIREWHWPAFNIADMAICMGVGIYLITSTWQVKHPRT"
     gene            3281..>3491
                     /locus_tag="PKK36_11395"
     CDS             3281..>3491
                     /locus_tag="PKK36_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNR95199.1"
                     /translation="MAHRIAAGRGLAILSADAMLVYRGMDIGTAKPSAAERASVSYLG
                     IDLADPSESFSAGAWLEHVRVSLPDA"
BASE COUNT          790 a          947 c         1090 g          664 t
ORIGIN      
        1 gaaggtggag gatctgcgcg accagctggt cgccaacaac gagacgatcc actgggtacc
       61 cggctatgtc ggaagcaagc gcttcggcaa ctggctgcag gacgccaagg attggaacat
      121 cagccgcaac cgcttctggg gatcctgtct gccgatctgg atttcagagg acggggcgga
      181 catgatctgc gtgggctcca tcgcggaact ggaaaaactt tcaggggaaa aggtgaatga
      241 tctccacaag cacatcgtgg accagatcac cttcgtgaaa gacggcaaaa cctaccgccg
      301 gacgccggaa gtgctggact gctggttcga gtcggggtcc atgccgtatg cccagcagca
      361 ttatcccttc gagcgcaagg acgagctgaa gaaattcttc ccggcgcatt tcatcgccga
      421 ggggctggac cagacgcgcg gatggttcta cacgctgctg attctctcga cggcaatctt
      481 caagaagagc tgctaccgca atgtggtggt caacggtctc atcctcgcgg aggacggccg
      541 caagatgagc aagcggctca agaattatcc cgatccggtg cacattctga acacctatgg
      601 tgcggatgct ctcaggcttt acatgattca ttcaccggtg gtccgggcgg aagatctgcg
      661 cttttcggaa gaaggcgtca aggatgcgct gcggcatctg ctgattccgc tctggaacgc
      721 ctacagcttc tttgtgacct atgctcgcgt ggatggatgg aagccttctg actccaacgg
      781 cagggctctt gccgggaacg cgcacctgct ggaccgctgg attctcagct cgctggatca
      841 tttgatcagc gaggtgaccg gcgcgatgga tgtctatgac ctgcagcagg ccgttcgccc
      901 gtttgtggcg ttcatcgagg accttaccaa ctggtacatc cgccgcagcc gccgccggtt
      961 ctggaaatcc acggatgacg aggacaaggc gatggcgtac cggacgctct ataccgtgct
     1021 gctgcagctc agcaaggtgg ccgcgccctt cattccgttc atcagcgaga cgatctaccg
     1081 caatctgcgg acagatgaca tgccggagtc cgtgcacctc tgcgatttcc cgcagagcag
     1141 cggggagaac cgtgacgagt cgctggagaa gcagatggcc gccgtcatga gcgtggtggt
     1201 gctgggccgc cagctccgct cggcgcacga cctgaaggtc cgccagccgc tgccggccat
     1261 tcatgtgatc agtcgcgatg cgtctcttct ggagcagatt caggaactga aggatatcat
     1321 tgcggaagaa ctgaatgtga agaacatctg gttcagcggg catgaagccg atctggtgac
     1381 gctgaaggcg aaacccaact tcaggaacct ggggccgcgg ctgggtccag cggtcaagca
     1441 ggccgccgct gcgatacaga aaatatcgca gcacgaactg gaatccgtta ttgaaggcag
     1501 gggaactgcg gccgtggaaa tcgcgggcca gaaacttgag ctggggctga cggacctgat
     1561 catcgagcgc ataccgcatc cggacatggt ggtggcctcc gagagggaac tggtggtcgc
     1621 tctggagacc gggctgtccg aagaactggt gcgggaaggg cttgcccggg agttcgtgaa
     1681 tcgggtgcag aacatgcgcc gcacgatgga tatggatgtc acgcagagga tcaggctccg
     1741 cttcacggga gacgaggcgc tgcgggaggc cctggatgaa catggcgaat atatccgcag
     1801 tgaaacactc gctctggacc ttgtttatgc gggcgaaacc gacgaaggga gcgaggctgc
     1861 ggacctgaac ggacatgctt gcagcattaa agtggaaata gtaaaatcat gaagttgaca
     1921 ggggataagg gctgtgatag acactgccac tcctgatggg cagaaaccag tgtggcgact
     1981 gcgaacaacc ggatattgag gtgttgaaat gagcgcgaaa aaagcaaaaa aagccgtgaa
     2041 aaagaaagcg gtttcggcaa agaagcctgc gaagaaagcc gccgtgaaaa aagcggcccg
     2101 gaagcccgcc gcccggaagc ccgtgaagaa gtctgtggca aagaaacccg tcaggaaatc
     2161 cgcagtgaaa aagcctgtga agaaggttgc ccggaaagcg gcaaagaagc ctgcgaagaa
     2221 agccgccgtg aaaaaagcgg cccggaagcc cgcggccaaa aagccggctg cgaaaaaagc
     2281 cgccgcctca aaaccggcga aaaagcctga agtgaagaag cctctggtca cggtgaaggt
     2341 gaaattcaca gccaagcaga tcaaaatctt ccgtcagcgc ctgcatgagc tgcgcgaccg
     2401 ggtggtggac gggatcaatt ttcttgccgg cgacaacctg acccgttcgc cgcgtgattc
     2461 cgccggtgat ctttccaact acagcctcca catggccgat cagggcaccg acaactttga
     2521 ccgcgagttc gccttgagcc agctgagcgg ggagcaggat gtgatctatg aaatagacga
     2581 ggcgctccag cgcctgaatg aaggctccta cggcgtttgc gaagactgcg gatgcgccat
     2641 cggccaggaa cgcctgctgg cgctgccgtt cgcccgccgc tgcatcaagt gcaaggcgat
     2701 ctatgagaaa gtgcacacga agtatcgtcc gcttgggcct cctgtggaca tgaagtagtt
     2761 tccggagccg gaatgcttgc cctgctgatt gctttattca tcactgcggc cgaccagatc
     2821 acaaaataca ttattcgcac agaatatgtc ctggccgaat cgcgcacggt catccccgga
     2881 ttcatggacc tgacctattt gcgcaacacc ggcgcggcgt ggggcatgct cggcgggcaa
     2941 aatgcctttc tcatcatctt ttcggtcgtg atgctggccc tgatgatcct tttccggaag
     3001 gcttttctca gcagcacact tgatcatcgt atcgctctgg gcctgatggc gggcggaatt
     3061 ctgggtaatc tgctggaccg tctccggctg gggtgggtga ctgatttcct ggacttctat
     3121 atcagggaat ggcactggcc cgccttcaat attgccgaca tggcaatctg catgggggtg
     3181 ggcatttatc tgataacctc gacatggcag gtgaagcatc cccgaacatg aacacagcgg
     3241 atggttcctg gccggaccta cagcggttgg gaaaacagcg gtggcgcacc ggattgccgc
     3301 cgggcgaggg ttggcgatcc tgtcggccga tgcaatgctg gtgtaccggg gcatggacat
     3361 tggaacggct aaaccgtctg ccgcagaacg ggcgtcggta tcgtatctgg gtattgatct
     3421 ggccgatccc tcggagtcgt tcagcgccgg cgcctggctg gaacatgtgc gcgtcagcct
     3481 cccggacgca c
//
LOCUS       DAOQWR010000197         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_28837_length_3428_cov_5.732286, whole genome shotgun sequence.
ACCESSION   DAOQWR010000197 DAOQWR010000000
VERSION     DAOQWR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28837_length_3428_cov_5.732286"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2945
                     /locus_tag="PKK36_11400"
     CDS             <1..2945
                     /locus_tag="PKK36_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNR95200.1"
                     /translation="TNRAEITASDVFDIEPTNDWAEVVIYPTLVSITSFRLLRGDSGL
                     VVEWVTGSENSTAGFHLERMDKSGSFVRVNEELLPALFGAPQGGIYQYPDRTGAASAY
                     RIVEVEYNGTERIYGPFNAEVEPAGGQRVSVFRSNAHTKPLKVRTMSGVKSAVPGPFS
                     RLKIPVRETGACFVSAADIAALTGRSEAEIVRMLGRAELKLGCDGEPVAYRAADNGMY
                     FFAEARETIYSDENVYILEEGAGLRVETAPGKPVPAASGRSFMDTRVYEQSVYAETAL
                     FDDPEEDFWLWDGLVAGHATLGRKIFMFDAADPASSGTAVLSVRLKGLTDTPAAADHH
                     ARISVNGALAAEGQWNGQDDFVLTAVLDAADLLPQANALQIEAVKDASVTHSTFYLDN
                     FELSWPRCYRAVQDQLILNADRNPVVTVEGFSSSGIDVWDVTQPKRPVRYRDLTPVAA
                     GGAWSISFRPAAPNCAYAVFAQARQVQPAGMPAADLKDGAIQCAYLVITDPELAETAA
                     LLVDYRRTQGLPGRVVSTEDIYNEFSEGIRTPHAINAFLKYAAENWAQPPQYVLLAGA
                     GSYDYRNFRGYGECLVPPLMVSTPRGLYASDNRLADLNGDKVPDISIGRLPAETPDEL
                     KRMISRIVSYEKGGDWKNGILMAADNADAGGRFPDDSDALALMIPSAYRIAKTYLGSL
                     NITVARNQLVGELNAGQGILHYIGHASLTQLAAEGLFRAAEQSRLTNDTRAAFAMIMS
                     CTAGRFDIPGYDGLTETLLKASAGGASAAWAPAVLAHNDQNVILSRSLLDGLLDKGDE
                     RIGDAVLRALGVYSASRRMDYMLDTFTLLGDPATAFSGRSAEASFEEWAWWHFGVDGV
                     QNPLQSGALADPDGDGNVNLMEFALNIDPKAMDGAVAVWIPSPSELNGKHPVFSYDRR
                     KAPAGTHYRLEVAGNLPASQWLSGPDAVQELTVQSLDETMERVAVWVNESLISGNAVF
                     VRLRVIMD"
     gene            2950..3423
                     /locus_tag="PKK36_11405"
     CDS             2950..3423
                     /locus_tag="PKK36_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNR95201.1"
                     /translation="MNMRYFILIAAVALGGPVPRPSAEGAAFFSMHPADISGLVLYTD
                     GETPAENVPVRVWDAHKREFILETETNEHGRYLLKALEPGEYYLTFDWTKLRLDIMPG
                     PGDPDRQNLGVVVVIPRPVGFVNVHMLNTMLIAGSLSERAVMLGDEERMNQVSSP"
BASE COUNT          681 a          906 c         1093 g          748 t
ORIGIN      
        1 tcaccaatcg cgcggaaatc acagcaagcg atgtttttga cattgaaccg acaaacgact
       61 gggccgaggt ggtcatctat cccacgcttg tttccattac ctcattccgt ctgctgcgcg
      121 gagacagcgg ccttgtggtg gagtgggtga ccggttccga aaacagcacg gccggtttcc
      181 atcttgagcg catggataaa tccggttcgt ttgtgagggt gaatgaagaa ctgctcccgg
      241 cgttgttcgg cgctccgcag ggcgggattt atcagtatcc ggatcgaacc ggcgcagcat
      301 cggcttatcg catcgttgag gtggaataca acggcaccga gaggatatac ggtccgttca
      361 atgctgaagt ggagccggcc ggcgggcaga gggtgagtgt gttcagatcg aatgcgcaca
      421 ctaaaccgct caaggtcaga acaatgtccg gagtcaaatc cgccgttccg gggcccttca
      481 gccgtctgaa aattccggtg cgtgaaacag gggcctgttt tgtaagcgcc gcggatattg
      541 ccgcgctgac gggccgttca gaagctgaga ttgtccgcat gctgggccgg gcggaactga
      601 agttgggttg tgacggtgag cctgtggcct atcgggcggc tgacaacggc atgtatttct
      661 ttgctgaagc ccgtgaaacg atctattccg atgaaaatgt ctacatcctg gaagagggcg
      721 caggcctccg tgttgagacc gcgcccggca aaccagtgcc ggcagcatcc ggacggagtt
      781 tcatggatac gcgcgtgtat gaacagtccg tttatgccga aacggcgctg ttcgatgacc
      841 ctgaagagga tttctggctg tgggatggcc tggtcgcggg tcatgcgacc ctgggcagaa
      901 agattttcat gtttgatgcg gccgatccgg catcttccgg aacggcggtt ttgtcagtgc
      961 gactcaaagg cctgactgac acccccgcgg ccgcggatca tcatgccaga atatcagtca
     1021 acggcgcact tgctgccgaa gggcagtgga acggacagga tgactttgtc ctgaccgccg
     1081 tgcttgatgc cgcggatctg ctgcctcagg ccaatgccct gcaaattgag gccgtcaaag
     1141 atgcgtcggt tacccacagc acgttttatc tggacaattt cgagctgagc tggccgcgct
     1201 gttatcgcgc tgttcaggat cagctgatcc tcaatgccga ccggaatccg gttgtgacgg
     1261 tggagggttt ttcatccagc gggattgatg tctgggatgt gactcagccg aagcggccgg
     1321 tgcgttaccg ggatttgact cccgttgcgg cgggcggcgc atggagtata tcgttccgtc
     1381 ccgccgcgcc gaactgtgct tatgcggtat ttgcacaggc gcggcaagtc cagcccgcag
     1441 gcatgcctgc ggcggatttg aaggacgggg caatccagtg cgcctatctg gtgatcacgg
     1501 atcctgaact tgcagaaaca gcggcactgc ttgtggacta caggcggact cagggcctgc
     1561 cgggccgggt ggtttccacg gaggatattt acaatgagtt cagcgaggga atccgcactc
     1621 ctcatgccat taatgccttc ctgaaatatg ccgccgaaaa ctgggcccag ccgccgcagt
     1681 atgttctgct ggcgggggcc ggatcatatg actacaggaa tttcagggga tatggtgaat
     1741 gcctggtgcc gccgctcatg gtttcaactc cgcgcggact ttacgcgtcg gacaacagac
     1801 tggccgacct gaatggagac aaggtccccg atatttcaat cggccgtctg cctgccgaaa
     1861 ctccggatga actgaagcgg atgatcagcc ggatcgtctc ctatgagaaa ggcggcgact
     1921 ggaagaacgg tatactgatg gccgccgata atgcggacgc cggaggccgg ttcccggatg
     1981 acagcgatgc gctggccctg atgatcccct cggcatatcg aatagcgaag acgtatctgg
     2041 ggagcctgaa catcacggtt gcgaggaatc agctggtggg cgaactgaac gcggggcagg
     2101 ggattcttca ctacattggt catgcatccc tcacgcagct tgcagcggaa ggccttttcc
     2161 gcgcagcgga gcagtcgagg ctcacgaatg acacgcgggc ggcctttgcg atgatcatga
     2221 gctgcacggc cggccgtttc gatatccccg gatatgacgg tttgacggag acgcttctta
     2281 aggcatccgc cggcggggct tctgcggcat gggctccggc tgtgcttgcc cacaacgatc
     2341 agaacgtcat cttgtccaga tctctgctgg acggcctgct tgacaagggt gatgagcgca
     2401 ttggcgatgc tgttctcagg gccttgggcg tatacagcgc cagccgcaga atggactaca
     2461 tgctggacac cttcaccctg ctgggcgatc cggccacggc tttctccggg cgctccgcag
     2521 aagcgtcctt tgaagaatgg gcctggtggc atttcggcgt ggatggggtt cagaatccgc
     2581 tgcagtcggg cgcgctggca gatcctgatg gagacggcaa tgtcaacctg atggaatttg
     2641 ctctgaatat tgatccgaag gccatggacg gggccgtggc cgtttggatc cccagtcctt
     2701 cggagctgaa cgggaaacac cccgtcttca gctatgaccg cagaaaggct ccggcaggaa
     2761 cgcactatcg tttggaagtg gcgggcaatc tcccggcgtc tcagtggctg tccggtcccg
     2821 acgctgttca ggaactgacc gtgcaaagct tggatgaaac catggagcgg gtggctgtct
     2881 gggtgaatga atcgctgatc agcgggaacg cggtttttgt tcgcttgaga gtgataatgg
     2941 attaggacga tgaacatgcg ttatttcatt ctgattgctg cggtggccct gggcgggccg
     3001 gtcccgcgtc ccagtgccga gggggccgcg tttttctcga tgcatccggc ggacatctcc
     3061 ggcctggtgc tgtacacgga cggcgagaca cctgccgaga atgttccagt ccgggtgtgg
     3121 gatgctcaca agcgggaatt tattctggaa acagaaacca atgagcatgg ccgctatctt
     3181 ttgaaggctc tggagcctgg ggagtactat ctgacctttg actggacgaa gctgcgtctg
     3241 gatatcatgc cgggaccggg tgaccctgac cggcagaatc ttggcgttgt ggtggtgatt
     3301 ccccggccgg ttggttttgt taatgttcac atgctgaata ccatgctgat tgccgggagc
     3361 ctttccgaac gtgccgttat gcttggagac gaggagcgca tgaaccaggt gagcagcccg
     3421 tgaacaga
//
LOCUS       DAOQWR010000198         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_28945_length_3419_cov_4.990785, whole genome shotgun sequence.
ACCESSION   DAOQWR010000198 DAOQWR010000000
VERSION     DAOQWR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28945_length_3419_cov_4.990785"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PKK36_11410"
     CDS             complement(<1..1470)
                     /locus_tag="PKK36_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNR95202.1"
                     /translation="MLAAIALAVFSYRDDTLLFERPSAWEQHYALKLGSVLDKDGLYA
                     VFPFDEPVIREPVRRGACGSEQTQCVPGLIGNARSFCGSNSAMIWTGVKWRDLGDAYS
                     ISMWVKIRNTSPMQAIMWWTGGSQKTGLMLVHGRMAFAVPGRASSVTISYPFEDYDRY
                     VFITAVVDRGRGFARLYQDGELRAEGSVSAVEQPNRYVVFGKMSSLKIGYPFCGELDE
                     SALWSRALSAEEVRQLHRRRRPLVADSGGRYAFKYRAAAAVRRILRALPCYLELFNPF
                     LHSSHLEALNLPVCDIILSGDDQRWFNSQHAWYLKNRTLKDDARRSRRVRMVLDGRAA
                     DVRMSLFYPPAMTSACHRMSYVVKLDSDITWRGMHSFVLLAPEDYGMLVPLLETALSQ
                     NYGLYSHSDGIAVLRLNRSVRGAYYFADYAENSFGSIHCGLDAGRLIQSIPADRRGIL
                     DIYDGLKNRYSPAFADDTLNGVSARETRFRIAYDRQTLEE"
     gene            complement(1541..2233)
                     /locus_tag="PKK36_11415"
     CDS             complement(1541..2233)
                     /locus_tag="PKK36_11415"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNR95203.1"
                     /translation="MNNLINVLQNAQDRFSAVDPVLYVLAVAVSLAASFAASMLYRFF
                     YENRATGSQVHRSFPLLGISVTTLFIAVQLSLPLSLGLLGALSIIRFRTPIKEPEEVG
                     FIMLVIASSIVCATLNFQYLLFLFAGAVAALVIQRHSNLVRHRFEDGIMIVSLAVAAE
                     SAALPQIIDYVRKNTKHSRLESTTSRDGRTSLQFAFAGMKLDMPAILSSLRGMEPSLE
                     ADIYFNRPGGLR"
     gene            complement(2226..2936)
                     /locus_tag="PKK36_11420"
     CDS             complement(2226..2936)
                     /locus_tag="PKK36_11420"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HNR95204.1"
                     /translation="MINRPLSAEDKYISPPFLYERIHAILRHYCRLDRLYPRNRVFSI
                     YYDTPSQTAFAEKTNGDFLKHKVRLRWYEDDQAGTPPPAQAVVYLESKNKVGGVRGKS
                     RQALLLDANQLRTASLEKPWLQAILAKHFREAGLASPGRWRPAIQIEYLRERYECPAS
                     GARVCVDSRIGCLRFNRQMIHGAAPVRLNTLVVEIKGVHNPDTPWLKLLHASGMRAAS
                     FSKYGACFAQLTGGLNHE"
     gene            complement(2941..>3419)
                     /locus_tag="PKK36_11425"
     CDS             complement(2941..>3419)
                     /locus_tag="PKK36_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine/uracil/vitamin C permease"
                     /protein_id="HNR95205.1"
                     /translation="CPRHHAMAVGIAIIPHVSDLLVKQWGSMMQALRDMGASSVPPAL
                     TDPELVKAMLQQGAYVTGHATVAGGAIVVGLLWGAASAALIDKQLKKAAGFFLAAALL
                     SYVGVIHSPQMQMALTSHVGHGYLLAAAVCFALGSSGKDRLQEISLPREDTGNPSV"
BASE COUNT          803 a         1002 c          941 g          673 t
ORIGIN      
        1 ctcctccagt gtctgccggt cataggcgat ccggaagcgt gtttccctcg cactgacccc
       61 attcaatgta tcgtcggcaa atgccggaga ataccgattc ttcaggccgt cataaatatc
      121 cagaataccc cgtctgtccg caggaatcga ctggatcagc cggccggcat ccagtccgca
      181 atgaatactg ccgaaggagt tttccgcata gtccgcgaag taatatgcac cgcggactga
      241 acggttgaga cggagcacgg caattccatc tgaatgagaa tacagaccat aattctgcga
      301 cagggcggtt tccagaagag ggacaagcat tccataatct tcaggcgcca gcaggacgaa
      361 agagtgcatg ccgcgccatg tgatatccga atccagtttc accacatacg acattcggtg
      421 acaggcggat gtcatggccg ggggatagaa aagagacatg cggacatccg ccgcccggcc
      481 gtcgagaacc atcctgaccc tgcggctgcg ccgggcgtcg tccttcagcg ttctgttttt
      541 caggtaccat gcatgctggc tgttgaacca ccgctgatca tcaccggaaa gaataatgtc
      601 gcagaccggc aggttcaggg cttccagatg gctgctgtga agaaaaggat tgaagagttc
      661 cagataacac ggcagggcgc gcaagattct tcgcacggct gcggcagcac ggtatttaaa
      721 ggcatagcgc ccgcctgaat ccgcaaccag cggcctcctg cggcggtgaa gctgccggac
      781 ctcttccgct gacagcgcac ggctccacaa tgcggattca tccagttccc cgcagaatgg
      841 atatccgatt ttgaggctgc tcatttttcc aaatacgaca taacgatttg gctgctcgac
      901 tgctgaaaca gacccttcgg ccctcaattc tccgtcctga taaaggcgcg caaaccccct
      961 tccgcgatca actaccgctg tgataaaaac atagcggtca taatcctcaa aaggatatga
     1021 gatcgtcaca ctggaagcgc ggcccggcac cgcaaaggcc atgcgtccat gcacaagcat
     1081 cagccctgtt ttctggctcc cgccggtcca ccacatgatt gcctgcatgg gcgacgtgtt
     1141 ccggattttg acccacatgg atatggaata ggcatcgccc agatcccgcc atttcactcc
     1201 ggtccagatc atggctgaat ttgatccgca gaatgaccgg gcatttccga taagacccgg
     1261 aacgcactgc gtctgttcac tcccgcaggc tccgcgccgg acaggctccc ggatgaccgg
     1321 ttcgtcaaac ggaaacacgg catacagacc gtccttgtca agcacgcttc ccagcttcaa
     1381 tgcatagtgc tgttcccatg ccgatggacg ttcaaacagc aaggtgtcgt cccggtaaga
     1441 gaagacggcc agcgcaatgg ctgcaagcaa caggattcgt cttgctaaac ggctgaaatc
     1501 acggactggg aataaaccgc tcatgcacga ccggcatcgt tcagcgcagc ccgccggggc
     1561 ggttgaaata gatatcggcc tccagcgagg gctccatgcc ccggaggctg gaaaggattg
     1621 caggcatgtc cagcttcatg ccggcaaagg caaactgaag gctggttctt ccatcgcggc
     1681 tggtcgtgct ttccaggcgg ctgtgcttcg tgttcttgcg aacgtaatct atgatttgcg
     1741 ggagggcggc tgattcagcc gccacggcga gggatacaat catgatcccg tcctcaaacc
     1801 ggtggcgcac aagatttgaa tgccgctgta tcaccagtgc ggccaccgca cccgcaaaga
     1861 gaaaaagcag atactggaaa ttgagcgttg cgcacacaat ggacgaagca atgacaagca
     1921 tgataaaccc aacctcctcc ggttccttga tcggtgtgcg gaaacggatg atggaaagcg
     1981 cgcccagcag tcccagcgaa agaggaaggg acagctgcac tgcaatgaac agggtcgtta
     2041 cggatattcc cagcagaggg aacgaacgat gcacctgact gccggtggcc cggttttcgt
     2101 agaaaaagcg gtagagcatg ctggccgcaa acgaggccgc cagtgataca gcaaccgcca
     2161 gcacgtaaag caccggatcc accgcactga agcggtcctg ggcattctgc agcacattga
     2221 tcagattatt catggttcaa tcctcctgtc agttgcgcaa agcatgcgcc gtattttgaa
     2281 aaactcgcgg ccctcattcc cgaggcgtgc agaagcttaa gccacggcgt gtcgggattg
     2341 tgcactccct taatttcaac caccagtgta ttcaggcgca ccggagcggc gccgtggatc
     2401 atctgacggt tgaaccggag gcagccgatt ctggaatcca cacagacgcg cgcgccgctg
     2461 gcgggacact catagcgctc ccgcagatat tcaatctgaa tggcgggacg ccagcgtccc
     2521 ggcgaggcca ggcccgcctc gcggaaatgc tttgccagaa tcgcctgcag ccagggcttc
     2581 tccagcgatg ctgtgcggag ctggtttgca tcaagcagca gcgcctggcg cgattttccc
     2641 cgaactccgc caaccttgtt tttggattca agataaacca ccgcctgcgc cggaggaggg
     2701 gttcccgcct gatcatcctc ataccatctc agccgcacct tgtgtttcag aaaatccccg
     2761 ttggtctttt ccgcaaacgc ggtctgcgag ggtgtgtcat aatagatgct gaacacgcga
     2821 ttgcgcggat agagcctgtc aagacggcag taatgccgca ggatcgcatg aatgcgttca
     2881 tacaggaaag gagggctgat gtacttgtcc tccgcggata atggccggtt tatcatgatt
     2941 tcaaacagac ggatttcccg tatcttcgcg aggaagggat atctcctgca gccggtcttt
     3001 cccggacgag cccagcgcaa agcatacggc ggcagccagc aaataaccgt gccccacatg
     3061 cgaagtgagg gccatctgca tttgcggaga atgaatcaca cccacataac tcagcagagc
     3121 ggcggccagg aaaaatccgg ctgctttttt aagctgtttg tcaatcagcg cggcggatgc
     3181 cgcgccccag agcagtccga ccacaatggc gccgccggcg acagtggcat gtcccgtgac
     3241 atatgcgccc tgctggagca tggccttgac cagttccgga tcagtcagtg cgggagggac
     3301 agacgacgcg cccatgtcgc gcagggcctg catcatgctc ccccactgtt tcaccagcaa
     3361 atcggaaaca tgcggtataa ttgcaatacc cacggccatc gcgtgatgcc tcgggcatg
//
LOCUS       DAOQWR010000199         3339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_29976_length_3339_cov_4.968940, whole genome shotgun sequence.
ACCESSION   DAOQWR010000199 DAOQWR010000000
VERSION     DAOQWR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29976_length_3339_cov_4.968940"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3339)
                     /locus_tag="PKK36_11430"
     CDS             complement(<1..>3339)
                     /locus_tag="PKK36_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNR95206.1"
                     /translation="AFQGFEGTGADTWSITAGAGSIQNLPGSIDTPVNERIRTGSYSW
                     QPGDTDEVEVALELGTVDVSAWSDVMIELHMSATCTNFSDGYGMYPSDSVTFSVALDG
                     GAYSAALTVSGNTETGDGIEGVLWAMDAAGIATATAGVFRSMAPTVGGIDPNGLATVR
                     IHIPEGTTSVNLKATVAQEYYGYYWNIDDISLNGVNDGGAADLPPSIAISPSGTNKTV
                     AVSNALSFVLSATEVPNDSGDTVRLWATGLPSGATFPEVSGTSILTNTFSWTPSATGT
                     TVVSFFAGDKDGTNQVSVRINVYEQQPVGTYRAVICGISDYDGTINDLTYCDDDAQDL
                     YDLLLTGSNWEAGNMQLLLNSQATEANIQAAIAAMGAAAQPGDVCLFFFSGHGGDDMP
                     DTDGDEGGDGYDEYMCPYYIMDNEITDDELGDWLDALPTDNIIVLLDTCHSGGHIKAP
                     AGLTPKGISRTGVVVTDRSNGFVDDLRKRGIKDSNDLTSPYIQTAADDDEYSYEDPAY
                     EHGIYTYFLLEALTNSDANADGWMAGEETFDYLYPKVVNAESTQHPQEYDGWNGLANI
                     LTWEPVADQPPQITLDPAGTNKTVVFDTALSFTVTATDGDGLQVSLGASGLPSGATFA
                     GATNTGTASSTFNWTPGEAQVGTYNVMFSATDDDGTTERGVKITVRDGSTAVDLFISE
                     YIEGSSNNKAIEIFNGTGSSVNLGTDGYVLRIYSNGSTTPSSITLSGTVADGDVYVVA
                     NSSANAAILAAADMTSGSLGFNGNDAVALAKSDVNIDVVGTIGNDAVFAADVTKVRKS
                     SVIEGVTTYNPSEWDDYAADTTSYLGSHTFDGGGSGGSTDLLPPVIQAASGVHAQQFD
                     ANWLASSNATGYRLDVATNEAFSAGGGGGNLMSNAGFETGDSTDWDKFEAGYSVVTAS
                     PQEGTYAVECVATARRDLMQAVDITGDGVTEYEISYYYKVSAGDDSDVRIWATWAAGG
                     QDSGDSLQPASYNATEADWTKIAYTVVPSSGANTLNYEIRVYNGATVYLDNFFVGVAG
                     GGGGGGGGSDFVPGYQNRDVGNVTTYAVTGLTEGVTYHYRVRAYNASSNSVNSGVTNV
                     TTGASTTPEITGFSVPAGATATVT"
BASE COUNT          653 a         1039 c          952 g          695 t
ORIGIN      
        1 gcgtcaccgt ggccgtggcg cccgccggca ccgagaagcc cgtgatttca ggcgtcgtgg
       61 aggcccccgt cgtcacgttg gtgacaccgg agttgacgct gttggacgag gcattataag
      121 cccgcacccg gtaatgatag gtcacgcctt cggtcagccc cgtcaccgca tacgtcgtca
      181 cattgccgac atcgcggttc tggtagcccg ggacgaagtc cgaacccccg ccgccgccgc
      241 caccaccgcc ggcaactccc acaaagaagt tgtccaggta gactgtggct ccgttgtaga
      301 cacgaatttc atagttgagg gtgtttgctc cgctgctcgg aaccacggta taggcgatct
      361 tggtccagtc cgcctccgtg gcattgtagg atgcaggctg cagactgtcc ccggaatcct
      421 ggccgccggc cgcccaggtg gcccaaatgc ggacatccga atcatcgccg gcggaaacct
      481 tgtagtagta gctgatttcg tactccgtga ctccgtcgcc cgtgatatca accgcctgca
      541 ttaaatcgcg gcgcgcagtg gccacgcatt cgacggcata ggttccctcc tgcggagatg
      601 cggtcacgac tgaatatccc gcctcgaact tgtcccagtc cgtgctgtct ccggtttcaa
      661 agccggcgtt cgacatcagg ttccctccgc cgccgccggc gctgaaggcc tcgttggtcg
      721 cgacatccag ccggtagccg gttgcattgc tggaggccag ccagttggcg tcgaactgct
      781 gcgcatggac tccgctggcg gcctgaatga ccggaggaag aagatcagtg cttcccccgc
      841 tgccgccgcc gtcaaacgta tggctgccca gatagctcgt ggtatccgcg gcgtagtcgt
      901 cccactcaga tggattatag gttgtcacac cttcgatcac cgaactcttg cggaccttgg
      961 tgacatctgc cgcaaagacc gcgtcatttc caatcgtgcc aaccacatca atattgacat
     1021 cgctcttggc cagggcaacc gcatcgttcc cgttaaagcc aaggcttccc gacgtcatgt
     1081 cggccgcggc gagaatcgcc gcattcgcag acgaattggc caccacatac acatcgccgt
     1141 cagccacggt gccggacagg gtgatggagg aaggggttgt gctgccattg gagtagatac
     1201 gcagcacata accatccgtt cccaaattca cagacgagcc cgtgccgttg aagatttcaa
     1261 tggccttgtt gttgctcgat ccttcgatgt actccgagat gaacaggtcc accgccgtgc
     1321 tgccgtcgcg cacggtgatt ttgactcccc gctcggtcgt tccatcgtcg tctgtggcgg
     1381 agaacatcac attataggtt ccgacctgcg cctcgccggg cgtccagttg aacgtcgagg
     1441 aagcggtacc ggtgtttgtt gcgccggcaa aggtcgcgcc ggaaggcaga ccggaagcgc
     1501 ccagcgacac ctgcagtccg tcgccgtccg ttgctgtaac cgtgaaactc agggccgtgt
     1561 caaacaccac tgtcttgttg gttccggccg gatcgagcgt gatctgcggc ggctgatccg
     1621 ccaccggttc ccatgtcagg atattggcaa gtccattcca gccgtcgtat tcctgaggat
     1681 gctgcgtgga ttctgcgttc accaccttgg gataaaggta atcaaacgtc tcttcgccag
     1741 ccatccagcc atcggcattg gcatcggaat tcgtcagcgc ctcaagcagg aagtatgtat
     1801 agatgccatg ctcataagcc ggatcctcat aagagtactc atcatcatcg gccgccgtct
     1861 ggatataggg agaagtcaga tcattcgagt ctttaattcc cctcttgcgg aggtcatcca
     1921 caaacccgtt ggagcggtcc gtgacaacga cgcccgtccg cgaaataccc ttgggtgtca
     1981 aaccagccgg ggccttgatg tgcccgccgc tgtggcaggt gtcgagcaga acgataatat
     2041 tgtccgtcgg cagggcatcc agccagtcac cgagttcgtc atcggtgatt tcgttgtcca
     2101 tgatgtaata gggacacatg tattcatcat agccatctcc gccctcgtcg ccgtccgtat
     2161 cgggcatatc atcgccgcca tgacccgaga aaaagaagag gcagacgtca ccgggctgag
     2221 ccgccgcacc catcgcggca atggccgcct ggatgttggc ttctgtggcc tgcgagttca
     2281 gcaggagctg catgtttccg gcctcccagt tggaaccggt cagcagcaga tcatagaggt
     2341 cctgcgcgtc gtcgtcgcaa taggtcaggt cgttgatggt cccatcatag tcggagatgc
     2401 cgcagatgac cgcgcggtag gtacccaccg gctgctgttc atacacattg attctcacgc
     2461 tcacctggtt tgttccgtct ttgtctcccg caaagaaact gaccacggtg gtgccggtcg
     2521 cggacggcgt ccaactgaag gtgttggtca gtatgctggt gcccgaaact tccgggaagg
     2581 tcgcaccgga cggaaggccg gtcgcccaaa gccggacggt gtccccggaa tcattcggga
     2641 cctcggtcgc cgacaggaca aaactgagag cgttgctgac ggcaactgtc ttattcgttc
     2701 cggacggact gatcgcgatg gaaggaggca gatccgccgc ccccccgtcg ttcaccccgt
     2761 tcaaggatat gtcgtcaata ttccagtagt acccgtagta ttcctgcgcg accgtcgcct
     2821 tgagattcac ggaggtggtt ccctccggaa tgtgaatccg aaccgttgcc aggccgttgg
     2881 gatcaatgcc gccaaccgtc ggtgccatcg agcggaaaac tccggctgtg gctgtcgcta
     2941 tgccggcggc atccatggcc cacagaacgc cctcgatgcc atcgccggtt tcggtgttgc
     3001 ccgaaacagt cagcgcggcg ctgtaagcgc ccccgtccag cgccacggaa aacgtcacgg
     3061 aatcagaagg atacatccca taaccgtcgc tgaagttggt gcaggtggcc gacatgtgca
     3121 gttcgatcat cacatccgac caggcggaca cgtccaccgt gcccagttcc aaagcgactt
     3181 ccacttcatc ggtgtcgccc ggctgccagg aatagctgcc ggtgcggata cgctcattga
     3241 ccggcgtgtc tatgctgccg ggcagattct gaatactgcc cgcgccggcc gtaatactcc
     3301 atgtatcagc ccccgtgcct tcgaaaccct ggaaagcaa
//
LOCUS       DAOQWR010000200         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_30351_length_3312_cov_2.697881, whole genome shotgun sequence.
ACCESSION   DAOQWR010000200 DAOQWR010000000
VERSION     DAOQWR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30351_length_3312_cov_2.697881"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1594
                     /locus_tag="PKK36_11435"
     CDS             332..1594
                     /locus_tag="PKK36_11435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR95207.1"
                     /translation="MNETYTSCMHQTIRGEARKRVFIFDINGLRGGSAQSLQALLNHI
                     APSRWEVILGFGRRGNRYRWSPYAVYEANMAGYDNYDFAPMTWNIRSAYGLFRWLVHL
                     PYDFVVSVCWLRRIRPDVVHLNVGQALTMGLAAHVLRLPVLWHVREMVCDNSSGRFQQ
                     WVYKTCAAKIVAISRAVVARLPACSQKVAVVPNGVDVASPALEDIDTFRKLHGLSASK
                     FTILLLGGLQISKGYLFLADVADRVAGESGIIFVLAGDTKDVPATGLHRLFRWLYRTM
                     RKTPGEKKLIQRRWRKIVEKGEAVFTGQVQSNIAIAASSIVVCPNLTTEPFGRTVIEA
                     YAQAKPVITANIPAFDEIVEEGVTGWMLPSKTDLWAAKLLELARNHEKVRVAGEFGLQ
                     KVRAFNGQAYADRIMSLYSDILHDQSLK"
     gene            1591..2667
                     /locus_tag="PKK36_11440"
     CDS             1591..2667
                     /locus_tag="PKK36_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR95208.1"
                     /translation="MNIALETNAPFVTRAGTARYVRGLLTGLHQITRPDVNWFELAWP
                     VENLDYRQPRRAWRTIYREMVWSRTTAVRELAKRHPDVIHLTGELNIIPPARIPVVRT
                     LLDCSAIRYPERFRRWQRNRGPSRLRCIPSADRVICISRFTADEGIRWLGLDPKKIDV
                     VHLGSDFGEAGTCRNEKQPSFRLPSIFFLFIGSLEPGKNLALLRETYLLAEQHGIDLP
                     PLVVIGVRPEGLSYEGAAPSNWQLVGWQPDEVLLYCLRRAMAFVFPTKYEGFGLPLLE
                     AMSVGCPAICSPVASLPEIGGDAVKYCEQTADAYLEAMSEICRGDHLRNQLSELGQKQ
                     ARKFSWRQCAEETIDSYRKSLGKY"
     gene            2669..3289
                     /locus_tag="PKK36_11445"
     CDS             2669..3289
                     /locus_tag="PKK36_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95209.1"
                     /translation="MGSLRLSFHLVAILVILFGALAGMLYVPGDAQVHLAVAEQFWLG
                     QPFRYNGVDGEMVVASTSPFWTLMIWAFFTMAGYAAPLLLKMIVLSIWLCVGFLIYRT
                     ARVLWDLPQSSSFAACMLWFGSCSVTINSLGGLENVLCALQLLLLCWISGPYAERPPG
                     WWRALILGAIGGWCVLTRLDGGMLAVLVIGIFFLHALISSARRNQA"
BASE COUNT          729 a          721 c          927 g          935 t
ORIGIN      
        1 tattgggtcg aaagacgcga aaaatattca cgctacccta tatccgtatt tgattgttat
       61 cttaaagagc tcggaacgga tggctattcc gaaaatgtca ttttcacaaa aggcggtacg
      121 cccactgatt cgccgatatt tccttgaagc aatgtgtgtc aaccggcgtg tgccacaagt
      181 tcacataagc attgtgaatt ccggcgcgta taattgtgtt tatgcgtctt atgattcata
      241 ttgaaccgat gctggattgg agtgagtcaa tattggcggg atgtcaatgc agaggtatgg
      301 gtatctatcc aaagcgagtc ggatttcttt catgaacgaa acatatacat catgtatgca
      361 tcaaacaata agaggtgaag cccgcaaacg ggtttttatt tttgatatca atggcttgcg
      421 cgggggatcg gcgcaaagtc ttcaagcatt gttgaaccac attgctccgt cgcgatggga
      481 ggtcatattg ggtttcggcc gacggggcaa tcggtataga tggtcgccat atgctgttta
      541 tgaagcaaat atggcgggct atgacaatta tgattttgct cccatgacat ggaatatacg
      601 cagtgcgtat ggcctgttcc gttggcttgt gcacctccct tatgattttg ttgtttctgt
      661 ttgttggttg cgccgcattc gccccgatgt tgtgcatttg aatgtggggc aggcgttgac
      721 aatgggattg gccgcccatg tattacgatt accggttctg tggcatgttc gggaaatggt
      781 ctgcgataac agctcggggc gttttcagca gtgggtttac aaaacctgtg ccgcaaaaat
      841 tgttgccata tcccgcgcag tagttgcgag gctcccagcc tgttcccaaa aggttgccgt
      901 cgttccaaac ggggtggatg ttgcttctcc cgcgcttgaa gatattgaca cctttcgaaa
      961 actgcatggc ctctcagcct caaaatttac gatcttgctg ttgggggggc tgcagatcag
     1021 caaaggctat ttgttcctcg ctgacgttgc ggaccgggtt gctggggaat ctggcattat
     1081 atttgtttta gcgggcgaca caaaggatgt tccggcaacg gggctgcatc gtctatttcg
     1141 gtggctgtat cgcacgatgc gcaaaacacc tggcgagaaa aaattaattc agcgccgatg
     1201 gcgaaaaata gtagaaaagg gagaggctgt ttttacaggg caagtccagt ccaatatagc
     1261 cattgccgca agttctattg tagtttgtcc caatctgacc acggaaccat tcgggcgaac
     1321 cgttattgag gcttatgctc aggcaaaacc cgtgattact gccaatatac cggcatttga
     1381 tgaaattgtc gaggaaggtg taacggggtg gatgcttccg tccaagaccg atttgtgggc
     1441 tgctaaactg cttgaacttg cacgaaatca tgaaaaggtc cgcgtggctg gggaattcgg
     1501 actgcagaag gttcgcgctt ttaacgggca ggcatatgcg gatcgcatca tgagtctcta
     1561 ttccgacatt ctgcatgatc aatcattgaa gtgaacattg ccctcgaaac caatgcgcct
     1621 tttgtgacac gtgccggcac tgcacgctat gtgcgtggtc tgctgactgg attgcatcaa
     1681 attactcgtc ccgatgtgaa ctggtttgag ctggcttggc ctgtagagaa cttagactat
     1741 cggcagccgc gcagggcatg gcgaacaatc taccgcgaga tggtatggtc gcgcacaaca
     1801 gcggtgcgtg aactggcgaa gcgtcatccc gatgtgattc acctcaccgg agaactgaat
     1861 atcattcctc ctgcgcggat tcctgttgtg cgtacattgc tggactgttc tgcgatccga
     1921 tatcctgagc gctttcgccg ttggcagcgg aacaggggac cctctcgtct gcgttgcatc
     1981 ccttctgcgg atcgtgtgat ctgtattagc agattcacgg cagacgaagg aattcgttgg
     2041 ttgggactgg atcccaagaa aatcgatgtg gttcatcttg ggagtgattt cggggaggct
     2101 ggtacttgca ggaatgagaa acaaccgtct tttcggctcc catcaatatt ttttctgttt
     2161 atcggatcat tggagccagg aaagaatctt gcgcttttac gcgagaccta tttattggcc
     2221 gaacagcacg gaatagatct ccctccattg gttgtcattg gtgtccgccc ggaggggctg
     2281 tcttatgagg gcgccgcccc atcgaattgg caacttgtcg gttggcagcc tgatgaagtt
     2341 ctgctgtact gccttcgacg ggcgatggcc tttgtatttc ccacaaagta tgagggattt
     2401 ggtttgccat tacttgaagc tatgtcggtg gggtgccccg caatatgctc gccagttgcc
     2461 agccttccag aaattggggg tgatgcggtt aagtattgtg aacagacggc ggatgcttat
     2521 ctcgaggcga tgagcgaaat atgcagagga gatcatctgc gtaatcaact gtctgaattg
     2581 ggacaaaagc aggcgcgaaa gttttcctgg cggcaatgtg ctgaagaaac aatagattcg
     2641 tatcggaaat ctttggggaa atattaacat gggaagttta cggctgtcat ttcacctcgt
     2701 tgccattttg gtgattctgt ttggtgcgct agccggtatg ttgtacgtcc cgggcgatgc
     2761 tcaagtccat ttggcggtgg cagaacagtt ctggcttggt caacccttcc gatataatgg
     2821 agtagatggc gaaatggtag ttgcctcaac cagtccgttc tggacgttaa tgatttgggc
     2881 cttttttaca atggcgggat atgcggcgcc cctattgctg aaaatgattg ttttgagtat
     2941 ttggctgtgt gtaggctttc tgatttatcg tactgcgcgt gtattgtggg acctgccgca
     3001 aagctcaagt tttgcggcat gtatgctctg gttcggcagc tgttcagtga caattaactc
     3061 tttggggggg cttgaaaacg ttctgtgtgc tcttcagctt cttcttctgt gttggatttc
     3121 tgggccttat gcggagagac ccccgggttg gtggcgcgcg ctgattcttg gcgcgatagg
     3181 aggatggtgt gtgctcaccc gactggatgg tgggatgctg gccgtgttgg taattggtat
     3241 attttttctc catgctctaa taagttcggc tcgaagaaac caggcctgat gctgatcaac
     3301 aatccctggg gt
//
LOCUS       DAOQWR010000201         3284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_30725_length_3284_cov_5.383710, whole genome shotgun sequence.
ACCESSION   DAOQWR010000201 DAOQWR010000000
VERSION     DAOQWR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30725_length_3284_cov_5.383710"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /gene="lepA"
                     /locus_tag="PKK36_11450"
     CDS             <1..1443
                     /gene="lepA"
                     /locus_tag="PKK36_11450"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNR95210.1"
                     /translation="EIGAADKPTLLVFNKIDLPSSDIEGTRKQIEDVLAIPAEDALCV
                     SAKTGEGVAEILQAIVERVPPPSGDPEGASRALVFDSIYDAYRGVVTYIRVVDGTFCP
                     GEKIRMMATGQDYEIKEVGKFVPQPVPVKMLGAGEVGYIIANIKEASEIRIGDTVTSA
                     RRPAAKALPGFKEIHPMVFAGLYPIDTSEYEKLKASLDKLRLNDCSFVYQAESSVALG
                     FGFRCGFLGLLHLEVIQERLRREYDLDLISTYPGVVYRVHLTSGEMIEVDNPVYLPET
                     TRIVAIEEPMVTASIICHNEHIGDMMQLIMDRRGILEHTHSMDTRRVMLTCKLPLNEI
                     LLDFHDRLKSVSRGYSSMDYEHAGYQPSDLVKLDILVNGDVVDAFSCIIHRSKAESRG
                     REICKSLKEVIPPQQFQIPLQAAIGRNIIARETIRAFRKDVTAKCYGGDITRKRKLLE
                     KQKEGKKKMKLVGKVSIPQDAFVSVLKANQ"
     gene            1446..2633
                     /gene="lepB"
                     /locus_tag="PKK36_11455"
     CDS             1446..2633
                     /gene="lepB"
                     /locus_tag="PKK36_11455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNR95211.1"
                     /translation="MRMNYFQKRKLRKLVKHYLYEARHLKHMREDILEPSVLAGLEEA
                     RQALVDAEKQGNPADTEKALAALCGRIEKACPNYTYPKVRENIEILVVAFAVAMAFRT
                     YFIQPFKIPTGSMQPTLYGITMHPAGKQTLLDVQPLRLAKFLVTGHSHVTIRAPISGR
                     IRFLGSARWPGEILLRTDKRGRFKIKRLDTTYNADIFNIENPALRMPDFPIPAAFEMH
                     VNSGDMVERGQVIATGQIRAGDHIFVNRVKYNFTRPKRGDVFVFDTSRINHPEIRAGT
                     FYIKRLVGLPGEVISIQPPYLVADGRKIEEPYPFHRLLTAEDQGYKGYHLAQYAPDRV
                     RPLLVTETDQIQLRDDEYLPFGDNTDFSLDGRFFGPINQRDIIGPAFTVYWPPTKRIG
                     RIQ"
     gene            complement(2956..>3284)
                     /locus_tag="PKK36_11460"
     CDS             complement(2956..>3284)
                     /locus_tag="PKK36_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95212.1"
                     /translation="SECASQRRYYITAVITLILRENMFPAPVSGEEPEIPGAGEQGGS
                     SPLPDDFQNHTAGKTRRHGSLYLVTAATPSREYESERDISSSAEERLRTAPGTRFAVR
                     LHSDWD"
BASE COUNT          752 a          927 c          917 g          688 t
ORIGIN      
        1 gaaatcggcg ccgcggacaa gcccacgcta ctagtgttca acaagattga cctgccgagt
       61 tcggatattg aaggaacccg aaaacagatc gaggatgttt tagccattcc ggccgaagac
      121 gccctttgcg tcagcgccaa aacgggggag ggggtggcgg agattctgca ggccattgtg
      181 gaacgcgttc cgccgccgtc gggcgacccg gaaggcgcca gccgcgcgct ggtgttcgat
      241 tcgatttatg acgcctatcg cggcgtggtc acctatattc gcgtggtgga cggaaccttc
      301 tgtcccggag aaaaaatacg catgatggcg accggccagg attatgaaat caaggaggtc
      361 gggaaattcg tgcctcagcc cgtgccggtg aaaatgctcg gggcagggga ggtgggctat
      421 atcatcgcca acatcaagga ggcctcggaa atccggattg gcgacaccgt aacctccgcc
      481 cgcaggccgg ccgcgaaagc gctcccgggc ttcaaggaaa ttcatccaat ggtcttcgcg
      541 ggcctctatc cgattgacac ctcggaatat gaaaaactga aggccagcct ggacaagctc
      601 cggctcaatg actgctcttt tgtttatcag gcggaaagct ccgttgcgct gggttttggt
      661 ttccggtgcg gtttcctcgg gctcctccat ctggaggtga tccaggagcg cctgcgccgc
      721 gagtatgacc ttgatctcat ttccacctat ccgggcgtgg tctacagggt gcatttgacc
      781 agcggcgaaa tgatcgaggt ggacaatccg gtttatctgc ccgagaccac ccgcattgtg
      841 gccattgagg agcccatggt cacggcatcc atcatctgcc acaatgagca catcggcgat
      901 atgatgcagc tcatcatgga tcgccggggc attctggagc atacgcattc catggacacc
      961 cgccgcgtca tgctcacctg caaactcccg ctcaatgaga ttctgctgga tttccatgac
     1021 cgcctgaaga gcgtcagccg cggatattcc tccatggact atgagcatgc cggatatcag
     1081 ccgtccgatc tggttaaact cgatattctg gtcaacggcg acgtggtgga cgcgttttcc
     1141 tgcatcatcc accgttccaa ggcggaatcg cgcggccggg aaatctgcaa gtcgctcaag
     1201 gaggtcattc ctccgcagca gttccagatc ccgctgcagg cggccattgg ccgcaacatc
     1261 attgcccgcg aaaccattcg ggccttccgc aaggatgtca ccgcgaaatg ctacggcgga
     1321 gacatcaccc gcaagcgcaa gcttctcgaa aagcagaagg aaggcaaaaa gaaaatgaaa
     1381 cttgtgggca aggtgagcat cccgcaggac gcctttgttt cggtcctcaa ggcaaatcag
     1441 taggtatgcg gatgaattac ttccagaaaa gaaaactgcg gaagctggtg aagcactatc
     1501 tttacgaagc gcgccacctg aagcacatgc gcgaggacat tctggagccg tccgtgctcg
     1561 ccggcctcga ggaggcgcga caggccctgg tggacgcgga aaagcagggg aaccccgccg
     1621 atacggaaaa ggcgcttgcc gcgctttgcg ggcggatcga aaaggcctgt ccgaactaca
     1681 cgtacccgaa agttcgtgaa aacattgaaa ttctggttgt tgcattcgct gttgcgatgg
     1741 ctttcaggac ctatttcata cagcctttca agatcccaac cggatccatg cagcccacgc
     1801 tgtacgggat caccatgcat ccggcgggca aacagaccct gcttgatgtt cagccgctgc
     1861 gcctcgcgaa attcctcgta acaggacatt cacacgtcac cattcgcgcg ccgatttcag
     1921 gaagaatacg ctttcttggc tccgcccgat ggcccggaga aattctgctt cgaaccgaca
     1981 aaaggggacg gttcaaaatc aagcgtttgg acacgacgta taatgccgac atcttcaaca
     2041 tagagaatcc ggcgctcagg atgcctgatt tcccgattcc ggccgccttt gagatgcacg
     2101 tcaacagcgg agacatggtc gagcggggtc aggtcattgc aaccggacaa atccgcgccg
     2161 gggaccacat atttgtgaac cgcgtgaaat acaacttcac gaggcccaaa cgcggtgatg
     2221 tgtttgtatt cgacacatcc cgcataaatc atccggaaat aagggcgggc actttttaca
     2281 tcaagcgcct tgtgggcctt ccgggcgagg tcatttccat ccagccgccg tatctggtgg
     2341 ccgacggcag aaagattgaa gagccttatc cgttccacag gcttctaacc gccgaggatc
     2401 agggctacaa gggatatcac ctggctcagt acgcaccgga ccgggtccgg ccgcttctgg
     2461 tgaccgaaac ggaccagatt caactcagag acgatgaata cctgccgttt ggagacaata
     2521 cggacttcag tctggacggc cggttcttcg ggccgataaa tcagcgggac atcattggtc
     2581 cggcattcac cgtatactgg ccgccgacaa agcgcatcgg acgcattcag tgaagttgag
     2641 agagccggcc gtttagtccg ctgaaaagag gtatcgttcg caaaatcccg gcctgacagg
     2701 tcaaaccgcc tgtttctggg gttattaaca taacgtcgca taatatatct tatgtctact
     2761 aatatatccg gaccgccaca atatgtagga ttcagatttg gaaccatatc aacaaggcca
     2821 ctgttaataa cctgcatccc agagcccgat cgcgcgctct atcacgctgc atccatgcct
     2881 ccttgggtat gtccgtgatg agtctgccgt gcctgcgagg cagtagcgca gttccggatc
     2941 tgaggtgaat atccttcagt cccaatcaga gtgcaggcgc accgcaaacc ttgtgcctgg
     3001 ggccgtccga agacgctctt cggcactgga ggaaatgtca cgctcgctct catactcccg
     3061 ggagggcgtc gcggctgtaa cgaggtacaa acttccatgc cgacgggttt tccccgctgt
     3121 gtgattttgg aagtcatcgg gcagggggct tgacccgccc tgttcccctg cccccggaat
     3181 ttccggttct tctccagata caggagctgg gaacatattt tcccggagaa tcagcgtgat
     3241 cacggcggtg atgtagtagc gcctctgtga ggcgcattct gaaa
//
LOCUS       DAOQWR010000202         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_31595_length_3221_cov_3.536008, whole genome shotgun sequence.
ACCESSION   DAOQWR010000202 DAOQWR010000000
VERSION     DAOQWR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31595_length_3221_cov_3.536008"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1856)
                     /locus_tag="PKK36_11465"
     CDS             complement(153..1856)
                     /locus_tag="PKK36_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95213.1"
                     /translation="MNTNRELLEKQSSAVTLSDMEIFIFPELLFSLVLANIMSPRIWE
                     WRKDKWFDKLDRMTPYRRILRLKQFIIDHYEFNLDLETWGLTSQQREVARFKEWIDEK
                     TLSESNALFGYEGDRYYFDMDIRKHFGLDQYTSDTIPYWKTETVEAMDAFRFKPGYSG
                     GAGECVSLAALYAAALFIICKVPLEDIFLMATPLHSQNFVDVKDGIITNNRRIVTKPM
                     WFNGTELTAKAQRALRNEQVTIVTHVSGHIHVMYPEATIDTAAMDRFTNKLSSFLTTT
                     VDMDILTSFLRFNRELQECFQVEHVCHGKPRYIPLNTAFLYEDSSAFRMDSQTRDKLL
                     ADIDEYKFFSAPIEGRISLNKFEDFFRKNKVDASRPDDVMKLVQSFNCEHSRAYEIME
                     ALMQFIHVEPRLPGREKTYVTRDPLEIDPEMSREQIEEYLLSVRGTHPVAGLAFHALR
                     DVARAGWEPFMKAALERNPVAVSGAAEMSDEEVVRLLDEFDGESIYDGNRLAQPDEVW
                     NFRRGDGLEKAICLANILKNRHPEQAVQIRCGNGRAELTCGTVCAAWDTAKGLSVELS
                     I"
     gene            1940..>3221
                     /locus_tag="PKK36_11470"
     CDS             1940..>3221
                     /locus_tag="PKK36_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95214.1"
                     /translation="MTYIARQMTSDDHNDGADLLLNLNFEPDWAKKAPSVNSCAGGDR
                     DEERPRRPRRDRPPRDARDRDRRPPRRDHSGERRERLRDPQRAPRPPRVEPLNAEVHF
                     LPEKNNIGAVVHLVRARKQAFPLFGLASIFLSDARHYLIKLTAPKGQSLEFHQCGVCQ
                     LVFLNKADLEQHVLAAHLDVHFAVEEQETEPPAGKFVCVGRCPKTGLLLGPPNYHGFN
                     AALQELHAKHYAGMSLQDCRARIEMIHDQDVIEEWRNSCRRTTVYRLKSAPADAPGLT
                     RDQAEALLREKHLNRMMHSRSRVIMPAALIAQLPDESLTARIRQAWNRETKRPFTLAL
                     ALQPALKHMGLHVFRSKGSDHYVSAIKPSPIDPGHAVPLIRSILEFVESHPDSKLPAV
                     LEALAPGGAADSGETAELIQSVTWLIEKGHVIEYF"
BASE COUNT          611 a          914 c         1012 g          684 t
ORIGIN      
        1 aaagggccca aatgtggccc aaaatggcga aatcctttct tgtgttcacg cattttggcg
       61 tgatagaaag ttttctgtct tttcccatga agagtttgcc acagagttta agagcggcgt
      121 ttataaaagg ggaatgctct cgctgaaatt cttcagatgc tcagctcgac agacaggccc
      181 ttggcggtgt cccatgcggc acagacggtg ccgcaggtca gttcggcgcg gccgttcccg
      241 catcgaatct gaacggcctg ttccggatgc cggtttttca ggatgtttgc aaggcagata
      301 gctttttcaa ggccgtcacc gcgcctgaag ttccagactt cgtcgggctg ggcaagacgg
      361 tttccgtcgt agatggattc gccgtcgaat tcgtccagca gcctgaccac ctcttcgtct
      421 gacatttctg cggcgccgct cacggcgacg ggattgcgtt caagggcggc tttcatgaag
      481 ggctcccagc cggcgcgggc gacatcgcgc agggcgtgga aggccagccc ggcgacggga
      541 tgcgtcccgc gcacagaaag cagatattct tcgatctgtt cccggctcat ttccgggtca
      601 atctcgagcg gatcgcgggt cacataagtc ttttcgcgcc ccggcagacg cggttcgaca
      661 tggatgaact gcatcagggc ctccatgatt tcataggcgc gggaatgttc gcagttgaag
      721 gactgcacca gcttcattac atcgtcgggc ctgctggcgt ccaccttgtt cttcctgaag
      781 aaatcctcga acttgttcag cgagatgcgc ccctcgatcg gcgcgctgaa gaatttgtat
      841 tcatcaatgt ccgccaggag cttgtcgcgc gtctggctgt ccatgcggaa ggcgctggag
      901 tcttcataga ggaaggcggt gttgagcggg atatagcggg gcttgccgtg gcagacgtgc
      961 tcaacctgga agcattcctg cagctcgcgg ttgaagcgca ggaagctggt cagaatatcc
     1021 atgtccacgg tggtggtcag gaacgacgaa agcttgttcg tgaaacggtc catggcggcg
     1081 gtgtctatgg tggcttcggg atacatcacg tgaatgtgcc cgctgacatg ggtgacgatg
     1141 gtcacctgct cattgcgcag ggcccgctgg gccttggcgg tcagttcggt cccgttgaac
     1201 cacatgggct tggtgacgat gcggcggttg ttggtgatga tgccgtcctt cacgtccaca
     1261 aagttctgcg agtggagcgg ggtggccatc aggaagatgt cctcaagggg cactttgcag
     1321 atgatgaaca gggccgccgc gtaaagcgcc gccagcgaga cgcattcccc cgcgccgccg
     1381 gaatagccgg gcttgaagcg gaaggcgtcc atggcctcga cggtttccgt tttccagtag
     1441 gggatggtgt cgctggtgta ctggtcgagg ccgaagtgct tgcggatatc catgtcgaaa
     1501 taataccggt cgccctcata accgaacagg gcattgcttt cgctcagtgt cttttcgtca
     1561 atccactctt tgaaccgggc cacttcccgc tgctggctgg tgaggcccca ggtctccaga
     1621 tcgaggttga actcgtagtg gtctatgatg aactgcttga ggcgcaggat gcgccggtag
     1681 ggggtcatcc tgtccagctt gtcgaaccac ttgtctttgc gccattccca gatgcgcgga
     1741 gacatgatgt tcgccagcac aaggctgaaa agcagttccg ggaatatgaa aatctccatg
     1801 tcggagagcg tgacggccga tgactgtttt tcgagcagtt ctctgttcgt gttcatgggc
     1861 atcactttaa cgctccgcgg ggccgaatca atagcgaatg aggggccgcc gcgggaaaat
     1921 gctggaatcg gtctgtcgaa tgacgtacat tgcgaggcaa atgacatcag acgaccacaa
     1981 cgacggcgca gatctgctgc tgaatctcaa tttcgaaccc gactgggcga agaaggcgcc
     2041 ctccgtgaat tcctgtgcgg gcggggaccg ggatgaggag cgcccccgcc gtccccgccg
     2101 ggaccgtcct ccgcgtgatg cgcgcgaccg ggaccgccgg cctccgcgcc gggaccattc
     2161 cggggagcgt cgcgaacgcc tccgcgatcc gcaacgggct ccgcgcccgc cgcgtgtgga
     2221 gccgctgaac gccgaagtcc atttcctgcc ggagaagaac aacatcgggg ccgtggtgca
     2281 tcttgtccgt gcccgcaagc aggccttccc gcttttcggg ctggcttcga ttttcctttc
     2341 ggatgcccgg cactatctca tcaaactgac ggcgcccaaa gggcagtcgc tcgaatttca
     2401 tcagtgcggg gtgtgccagc tggtgttcct gaacaaagcg gatctggagc agcatgttct
     2461 cgctgcgcat cttgatgtgc atttcgcggt ggaggagcag gagaccgagc cacccgccgg
     2521 aaaatttgtc tgtgtggggc gctgcccgaa gacgggcctg ctgctgggac cgccaaacta
     2581 tcacggattc aatgcggcgc ttcaggagct gcatgccaag cattatgccg gcatgtccct
     2641 gcaggactgc cgcgcgcgga ttgagatgat tcatgatcag gatgtgattg aggaatggcg
     2701 gaactcctgc cgccggacga ctgtttaccg cctgaaatcc gccccggcgg atgcgcccgg
     2761 gctgacgcgc gaccaggcgg aggcccttct gagggagaag catctgaacc ggatgatgca
     2821 cagcaggagc cgggtgatca tgccggccgc gctgatcgcg cagctcccgg atgaatcgct
     2881 cacggcgcgg atccggcagg catggaatcg cgagaccaag cgcccgttca ctctggcgct
     2941 cgcgctgcag cctgcgctca agcacatggg cctgcatgtg ttccggtcga agggaagcga
     3001 tcactatgtg tcggccatca aacccagtcc gattgatccc ggccatgccg ttcccctgat
     3061 acggtccatc ctggagttcg ttgaatcgca tccggattcc aagctgccgg cggtgctgga
     3121 ggccctcgcg cccggcggcg cggccgactc cggggagacc gccgaactga ttcagtctgt
     3181 aacatggctg attgaaaaag gacatgtgat cgagtatttt g
//
LOCUS       DAOQWR010000203         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_31959_length_3196_cov_6.046164, whole genome shotgun sequence.
ACCESSION   DAOQWR010000203 DAOQWR010000000
VERSION     DAOQWR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31959_length_3196_cov_6.046164"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..819)
                     /locus_tag="PKK36_11475"
     CDS             complement(106..819)
                     /locus_tag="PKK36_11475"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNR95215.1"
                     /translation="MKKLLYAMIIIAAAAAASAYDDGDSQAWIKLGLSGKLLSNVTVK
                     AESEFRYGDDASEFYEQYTLLGVDYTATEWLKIGLAHQEIASLKQTANWSQKVTDGME
                     SYSKVSDTYWTQEERPTLDITLSEKLAGWGLEDRVRVEYRMKENEDEYFRYRNRIKAK
                     TPWKWTEYKINPYAAWEVYYSDKDTDPDWDRHRFYAGATFNLYKALKADLYYCLQRDL
                     KSGDWTDVNVAGLGISAAF"
     gene            complement(819..1763)
                     /locus_tag="PKK36_11480"
     CDS             complement(819..1763)
                     /locus_tag="PKK36_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNR95216.1"
                     /translation="MKYCMIITALLLSLAGPLPAETPLEGTLSFSGAWALYPMAVRWA
                     EEFQKLHPGVRFDISAGGAGKGMTDCLAGAIDVGMVSRDIAPAEAENGAFGISVAKDA
                     VVPVINAGNPLLKTLLTKGITQDGAKSIWITGTAKSWRDVTGQELKAPLRVYTRSDAC
                     GAAETWAKYLGGRQEDLKGIGVYGDPGTGEAVRKDPLGVGYNNVNYAYDAKTQRPVAG
                     LVVLPLDVDGSGSIEPNEDFYKTRKELTDAIADGRYPSPPARNLFFVTKGRPGNPLLT
                     AFIEWVLTDGQQFVQEAGYINLSQEVLQNQIKKLKEVE"
     gene            complement(1760..2194)
                     /locus_tag="PKK36_11485"
     CDS             complement(1760..2194)
                     /locus_tag="PKK36_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR95217.1"
                     /translation="MKLTTKSEYALLALLHMARQDPSAWTSVDAVARAKNIPPKFLEQ
                     ILLALKRARLLRSTKGKHGGYQLAKPAGQISIAEIVRVLDGALAPVESVSEYFYESTP
                     IEGEPGLVRVFKKIRDYASDLLESTTLADICRNRPLKRRRTK"
     gene            complement(2281..>3196)
                     /locus_tag="PKK36_11490"
     CDS             complement(2281..>3196)
                     /locus_tag="PKK36_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNR95218.1"
                     /translation="IRAVFPNVAKGGITSCRSAVLPMEAKNGDDPFREYIAAAREAGL
                     EVHAWIICWSLDTAPADFAEQMKKEGRLVIGADGKTRPWLNPAHEANRNMLAAFIREV
                     IRSYNPEGIHLDYIRYPDSDGSYDPVSRRQFEKQTGTAVAQWPRDVLAGGLLRRAWLT
                     WRAADLTALVARLHDEVRAENPRVRLSAAVYGNWPDCADSIGQDWGRWLKDGRLDFVC
                     PMNYTSKPGAFDSLLGKQMRLPGAQKRLYPGIGVSATESQLTPDLVIEQIVTARGREA
                     PGFMLYQLDEVLTEDVLPVLRLGVTRPR"
BASE COUNT          570 a          953 c          962 g          711 t
ORIGIN      
        1 gctgacggcc agcgggaggg ccatggcgca caccgccagg gtgaacaggc ggaaggtgtg
       61 ccgcgccgca gcatctttga taatccggaa tgccatattc ttctcctaga acgccgcgct
      121 gattcccaga ccggccacat taacgtctgt ccagtctccg cttttcaggt cgcgctgcag
      181 gcagtagtac aggtcggcct tcagggcctt gtacaggttg aaggtggcgc cggcgtaaaa
      241 gcggtggcgg tcccagtcgg gatccgtatc cttgtcactg tagtaaactt cccatgccgc
      301 gtagggattg atcttgtatt cagtccattt ccacggggtt ttggccttga tgcgattccg
      361 gtaccggaaa tattcatcct cgttttcctt catgcggtat tccacgcgaa cccggtcttc
      421 caggccccag ccggccagtt tttcgctcag ggtgatatca agggttgggc gctcctcctg
      481 ggtccagtag gtgtcgctga ccttggaata ggattccatt ccgtccgtga ccttctgcga
      541 ccagttggcg gtctgcttca ggctggcgat ctcctgatgg gcgagtccga ttttcagcca
      601 ctccgtggcg gtgtagtcga cgcccagcag ggtgtactgt tcgtaaaatt cgctggcgtc
      661 gtcaccatag cggaattcgc tttcggcctt cacggtcacg ttggacagca gcttgccgga
      721 aagaccgagc ttgatccacg cctggctgtc gccgtcgtcg tacgcgctgg ccgctgcggc
      781 cgcagcgata ataatcatgg catataacag tttcttcatt attcaacctc tttcagcttt
      841 ttgatttgat tctgcaggac ttcctgcgaa agattgatgt agcccgcctc ctgcacgaac
      901 tgctgtccgt cggtcagaac ccattcgatg aatgcggtca gcagcggatt gcccgggcgg
      961 cctttggtca caaagaacag gttgcgcgcg ggcggggagg gatagcggcc gtcggcgatg
     1021 gcgtcggtga gttccttgcg tgttttgtag aaatcctcgt ttggctcgat gctgccgctg
     1081 ccgtccacgt cgaggggcag cacgaccagt ccggccacgg gccgctgggt ttttgcgtca
     1141 taggcatagt tcacattgtt gtagccgacg ccgagcggat ccttgcggac cgcttctccg
     1201 gtgcccgggt ctccgtaaac gccaatgccc ttgaggtcct cctgccgtcc gcccagatat
     1261 ttggcccatg tttcagccgc gccgcaggcg tccgagcggg tatagacccg cagcggtgcc
     1321 ttcagttcct gtccggtcac gtcgcgccag ctctttgcgg ttccggtgat ccagatggat
     1381 ttggccccat cctgcgtgat gccctttgtc agcagggttt tgagcagggg gtttccggca
     1441 ttgatgaccg gcaccacggc gtccttcgcc acggatattc caaacgcgcc gttttccgct
     1501 tcggccgggg cgatgtcgcg cgacaccatg ccgacatcaa tggcgccggc gaggcagtca
     1561 gtcattcctt ttccggctcc gcccgcggaa atgtcaaagc ggacgccggg atgcagtttc
     1621 tgaaactcct ccgcccagcg gacggccatg gggtagagcg cccatgcgcc tgaaaagctc
     1681 agcgttccct cgagcggagt ttccgccgga agcggtccgg cgagactcag cagcagtgcg
     1741 gtgatgatca tgcagtattt cattttgttc ttctcctttt taatggccgg ttgcggcaaa
     1801 tatcagcaag agtggttgat tccaggagat cggaagcata atcccggatt ttcttgaaca
     1861 cgcggacaag gcccggttca ccctcaatgg gggtggactc ataaaagtat tcgctgaccg
     1921 actccacggg ggcaagggcg ccgtccagca cgcggacgat ttctgcaatg gaaatctgcc
     1981 cggcgggctt ggcgagctgg tagcctccgt gcttgccctt ggtgctgcgc agcagccggg
     2041 cgcgcttgag cgcaagcagg atttgctcaa ggaacttcgg gggaatgttt ttggcccgcg
     2101 caacggcatc cacggaagtc catgccgagg gatcctggcg ggccatgtgc agcagggcca
     2161 gcagggcgta ttcacttttg gttgtcagct tcatcggccg ctccttttat taatatgact
     2221 aaaacaatag gacatatggg attaatgtcc agaagaaaat ataaatattt ttcgggcggg
     2281 tcagcggggg cgggtgacgc ccagcctaag caccgggagc acgtcctctg tgagcacctc
     2341 gtcgagctga tagagcatga atcccggggc ctcccggccg cgggcggtga cgatctgctc
     2401 tatcaccagg tcgggcgtga gctggctctc cgtcgcggaa accccgatgc cgggatacag
     2461 ccgcttttga gcgccgggca ggcgcatctg cttcccgagc agcgagtcga aggccccggg
     2521 tttcgaggtg tagttcatcg gacagacgaa gtccagccgc ccgtccttca gccagcgccc
     2581 ccagtcctgt ccgatgctgt ccgcgcaatc cggccagttc ccgtacacgg cggctgacaa
     2641 acggacgcgg ggattttccg ctcgaacttc atcatgaaga cgggccacca gcgcggtcag
     2701 atcagccgcg cgccacgtca gccaggccct gcgaagcagt ccgccggcca gaacatcacg
     2761 cggccattgt gcgaccgcag ttccggtctg cttttcaaac tgccggcggg aaaccggatc
     2821 atagcttccg tccgagtccg gatagcggat gtaatccaga tgaatgccct ccggattgta
     2881 actgcggatg acctcgcgaa tgaacgcggc cagcatgttt cggttggctt catgcgccgg
     2941 gttgagccac gggcgggttt tcccgtccgc tccgattacg agccgaccct cttttttcat
     3001 ctgttccgca aaatccgccg gcgcagtgtc aaggctccag cagataatcc atgcatgaac
     3061 ctccagcccc gcctcgcggg cggcggcgat atactccctg aagggatcgt ctccgttttt
     3121 tgcctccatg ggcagcacgg cgctgcggca ggaggtgatg ccgcctttgg ccacattggg
     3181 gaaaactgcg cgtatc
//
LOCUS       DAOQWR010000204         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_32228_length_3177_cov_4.808136, whole genome shotgun sequence.
ACCESSION   DAOQWR010000204 DAOQWR010000000
VERSION     DAOQWR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32228_length_3177_cov_4.808136"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..357)
                     /locus_tag="PKK36_11495"
     CDS             complement(199..357)
                     /locus_tag="PKK36_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95219.1"
                     /translation="MAEYGLLASKSAEFLTGIFGRLHGFWDAIPYGTSLAAAAGIAVV
                     VYLTFRKR"
     gene            complement(451..690)
                     /locus_tag="PKK36_11500"
     CDS             complement(451..690)
                     /locus_tag="PKK36_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNR95220.1"
                     /translation="MKRKIKYTDETMGKTRVVRNFLPPPEKLVLKESRVKITISLSKA
                     SVDFFKQEAKKHRTSYQKMIRKVIDVYTEQYRKTA"
     gene            complement(668..952)
                     /locus_tag="PKK36_11505"
                     /pseudo
     CDS             complement(668..952)
                     /locus_tag="PKK36_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            complement(1065..2879)
                     /locus_tag="PKK36_11510"
     CDS             complement(1065..2879)
                     /locus_tag="PKK36_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR95221.1"
                     /translation="MSRKQESTLSVTRRDVLKMMGVGALASAVGGGVFDSVAAGAAAS
                     SRAKPARTVGGPYNILFILTDQERYFRPGELPKDYRLPAHERLAKNGVVFENHRINSC
                     VCTPSRSVLYTGRHIQQTKMFDNTNFPWISSMSTDLRTVGDLLREAGYYTAYKGKWHL
                     TKEFETVNELGTPTKIFTKEMEAYGFSDYFGVGDIIAHEQGGYLHDGIISAMGVNWLR
                     GRGMELAAQRKPWFLAVNFVNPHDVMFINTDRPGEPVQARNILGRIVPEPKHPLYAKQ
                     WAFNLPATYGQPLDAPGRPAAHVDYLKSHDALVGHIANEDWRWRRRHNYYLNCLRDAD
                     RNIVPLLEELDALGLASNTIIMMTADHGDLDGAHRLHSKGATSYREQNNVPLIVVHPA
                     YPGGKRCKAVTSHLDIAPTLVGLTNTVSDEKAKITKGLPGKDFSPLLAAPEKAGHGAI
                     RDGVLYCYNMLAYIDGDFLEKVVGLLKQPNGKAIVQEAAKKGELQPNLMKRGAIRSVF
                     DGRYQFTRYFSPKQHNRPTSVEELFKMNDVELFDLQTDPEERYNLAIDRKKAAGLLEA
                     MNKKLNALLDSEVGEDVGQMLPGGVDGGWATTDAARDL"
BASE COUNT          606 a          964 c          885 g          722 t
ORIGIN      
        1 gccacaggta aaagaccgcc gccgcgatga gggcaagcca gatcagacgg gtgaggatgg
       61 cagaacgctt catgctcaca aacctcgggg tcaggcctga ttattgacat ttggctcaat
      121 gaatctcagc cccgacacag tttctgaggg tgatgtcagt tggtcataag gcttacccta
      181 atttccctct tgtctgagtc accgcttccg gaaggtcaga taaaccacga cggcaatccc
      241 cgctgcagcc gcaagagagg tcccataggg gatcgcatcc caaaacccat gcagccgccc
      301 gaagatgccc gtcaggaact cggccgactt cgacgccaga agaccatatt cggccattcg
      361 ctcctcagcg ccagaaggaa tgattcatga ttgcggtccc ggacgtgtga caccagggct
      421 tcgcaatttc cgccaacacc catgccgggc tcaagccgtt ttcctgtact gttccgtgta
      481 gacgtcgatg accttgcgga tcattttctg ataggacgtg cggtgctttt tggcctcttg
      541 cttgaagaaa tcgacgcttg ccttgctcag cgagatcgtg atcttgaccc tgctctcctt
      601 caggacgagt ttctccggcg gcggcaggaa gttcctgacg accctcgtct tccccatggt
      661 ctcgtctgtg tatttgatct tccgtttcac agatccgttt cccctttctc cggtatcccg
      721 ccccgatgat ccgggcagcg cagtccctgc agctaaagcg gaccgtgatg attccttcgt
      781 tgaccctgcc catagagaga gaagcgcgcc tcgtcacggc gatggctgac gtcctccacg
      841 atcacacgaa gccaatcgag aaaagcttgt tgggccaggg aaaggcgaca ccgtgcttgg
      901 ctttaatttc cagattcttt ttctcatccc ggccgaacga tggcttttcc atacgagaag
      961 attaacagag gcatgcaaat tcagcaatac ttatatattg caaatgatat ggcgaatagc
     1021 atcgaagtgg agtaatcgca ataatcaggc ctgaccccga tgcttcacaa atccctggct
     1081 gcatcggttg tcgcccagcc tccatccaca ccgcccggga gcatctgccc tacgtcctca
     1141 ccgacttccg agtcgagcag cgcattcagc tttttattca tggcttcaag aaggccggcg
     1201 gccttctttc gatcgattgc caggttgtag cgctcctccg gatcggtttg caggtcgaac
     1261 agctccacat cgttcatttt gaagagttcc tcaaccgacg tcggccggtt gtgctgcttc
     1321 ggcgagaagt agcgcgtgaa ctggtaccgc ccgtcaaaga cgctgcggat ggcgccgcgc
     1381 ttcatgagat ttggctggag ttcgcctttc ttggcggcct cctggacgat tgccttgccg
     1441 tttggctgtt tcaggaggcc tacaaccttt tcgaggaagt cgccgtcaat gtaggcaagc
     1501 atgttgtagc agtacaggac gccgtcacgg atagctccgt gcccggcctt ctcaggcgca
     1561 gcgagcagcg gcgagaaatc cttcccgggc aatccctttg tgattttcgc cttctcgtcc
     1621 gagacggtgt ttgtcaaccc gacgagggtc ggcgcgatgt cgaggtgcga tgtcaccgcc
     1681 ttgcatcgct ttccgcccgg gtaggctgga tggacaacga tcagcggaac attgttctgc
     1741 tcgcgatagg atgtcgcgcc cttcgagtga agccggtgcg cgccgtcgag atcaccgtgg
     1801 tccgcggtca tcattatgat tgtgttcgaa gcaaggccca acgcgtccag ttcctccagc
     1861 aaaggcacga tgttgcggtc ggcgtcgcgc aggcaattga ggtagtagtt atgccgcctt
     1921 cgccagcgcc agtcttcgtt tgcgatgtga ccgaccagcg cgtcgtgtga tttgaggtag
     1981 tcgacatggg cggcgggccg gcccggcgca tcaagcggct gcccataggt cgccggcagg
     2041 ttgaacgccc actgcttcgc atacaacgga tgcttcggct ccggaacgat gcggccgagg
     2101 atattccgcg cttggacggg ctcgccggga cggtcggtat tgatgaacat gacgtcgtgc
     2161 gggttgacga agttcaccgc gagaaaccac ggtttgcgct gcgccgcaag ttccattccc
     2221 cgcccgcgca accagttcac acccatggcc gagatgatcc cgtcgtgcag gtagccgccc
     2281 tgctcgtgcg cgatgatgtc gcccaccccg aagtaatccg agaacccgta agcttccatc
     2341 tctttggtga agatcttcgt cggagtgccc agctcattga ccgtttcgaa ttccttggtg
     2401 aggtgccact tgcccttgta ggcggtgtag taacccgctt cgcgcaacag atcgccgacc
     2461 gtgcgcagat cggtagacat gctgctgatc cacgggaagt tcgtgttgtc gaacatcttc
     2521 gtctgctgga tgtggcggcc ggtgtagagc acggagcgcg acggcgtgca cacgcatgag
     2581 ttgatccggt ggttctcgaa cacgacgccg tttttcgcaa gacgctcgtg ggcgggcagc
     2641 cggtagtctt tcggcagctc gcccggacgg aaatagcgct cctggtcggt caggatgaag
     2701 aggatgttat acggcccgcc gaccgtcctt gccggcttgg cacgcgaaga cgccgccgcc
     2761 cctgcggcta cgctgtcgaa aacgccgcct ccgacggctg aagccagggc ccccaccccc
     2821 atcatcttca gaacatcccg gcgggtgacg gaaagcgtag actcctgttt gcggctcatg
     2881 attcaccctc cctcctgctt atccatccgc ggggcgagac tggggcagtc aatagtcagt
     2941 cttggctcag ctgcttctcc cgttatgttg aaatacaaga cgcgacccca tttcctggcc
     3001 attgtatcct tccgagttca ggcggtcagt tcttgcatga tgcgtttctc caactattca
     3061 cgcttcaaga cctaccctca tcttcctatg ttgaaataca agacctgacc ccatttcctc
     3121 ctctcgatcg tggccttctc gtcggcggcc aggtcccccc gcgccgccac ggtgcgc
//
LOCUS       DAOQWR010000205         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_33024_length_3127_cov_3.576497, whole genome shotgun sequence.
ACCESSION   DAOQWR010000205 DAOQWR010000000
VERSION     DAOQWR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33024_length_3127_cov_3.576497"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PKK36_11515"
     CDS             complement(<1..404)
                     /locus_tag="PKK36_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNR95222.1"
                     /translation="MKRVNRLVLRAGFVFAAAVVVVALAASGCSTRKKREIGESSIGG
                     LTGSDIYGNPLPARSGSPVLCYSPALMLPEMNTEQYSHIKGNSFKNVKNEPLSTFSID
                     VDRASYANVRRFLNDGNMPPPDAVRIEEMINY"
     gene            complement(423..1802)
                     /locus_tag="PKK36_11520"
     CDS             complement(423..1802)
                     /locus_tag="PKK36_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95223.1"
                     /translation="MKSDFKEGRRGYAFKNGKLYSFEPRHVLVMTIWPDPRAWFKRRS
                     HNWRPTRKLADKLLSTNLYRAGAIGVDVPDTDPLGQRLLPGIYAETEHCLVQNWRRDM
                     KVMGGCFDMIPADIRNELLKYSERKWHLLNMFARCPGAMDLSHSNPALLYALASNWVF
                     HKPAVQKPVRAARSLMSRKQKHILSWLGFPATETARKILSKIMPRSLSINALLYLRDS
                     FNSPEVVKMLSHVNRINAGILRLATDARYRQFITSRLLEDLAEDDTQDAAQPPISAFF
                     EYTLRMMDKVPGHHVRKFVSLDRLRAVHDELAARVPPALMEEYRMPFPSSFFDPPFAG
                     TEDIQPLTTPRALYFEGEIMDHCAGSYTEMVTRGSHYIYRVASPVRATLSIYRCGKNW
                     RAGQLYMAHNKRVDAPMREYLFTELFRSGQPQPSPSWYGWGWEMGEWMDGIYPRPYFL
                     FPHFIFNNH"
     gene            complement(1853..2341)
                     /locus_tag="PKK36_11525"
     CDS             complement(1853..2341)
                     /locus_tag="PKK36_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNR95224.1"
                     /translation="MIVPLTAIAIIISTTGCTSSKARSRRSDIDPRGTEVWNPQNVQR
                     PGDNVEYTYLCDPIYFAYDSSQIWASERTKIENVAESLRQNIKTGVIIEGHCDERGSR
                     EYNMALGERRALAVRSYLTGLGIDGSRIYTKSFGEESPDVSGHDEQSWGLNRRAEFVL
                     VD"
     gene            2256..2441
                     /locus_tag="PKK36_11530"
     CDS             2256..2441
                     /locus_tag="PKK36_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95225.1"
                     /translation="MSDLRDLALLEVQPVVEIIIAMAVSGTMIPVFDVMQPTIFIIPF
                     PLIIGIIAIYVILHGEE"
     gene            complement(2522..>3127)
                     /locus_tag="PKK36_11535"
     CDS             complement(2522..>3127)
                     /locus_tag="PKK36_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR95226.1"
                     /translation="PGNLRTMPYSIVLLDEIEKAHPRVLDIFLQLFDEGRITDGKGRT
                     SDARNAIFIMTSNLWASSGARAAPLGFNQPSEKAGEAANPAKGELKKHFRVEFINRID
                     EIITFTPLGNDAILAIAKNMLKMLAQAVRKKHKIQLHYDYAVLAALCKSANSEEFGAR
                     NLRRVVQESIEIPLAKHLSLGSPRGDVCICCDPETGNIAVK"
BASE COUNT          747 a          868 c          761 g          751 t
ORIGIN      
        1 aaatagttaa tcatttcctc aatgcgcaca gcatcaggcg gcggcatatt cccgtcattc
       61 agaaatctcc tgacattggc gtaggatgcg cggtcaacat caatcgagaa ggtcgaaagc
      121 ggctcgtttt tcacgttttt gaatgaattg cccttaatgt gactatactg ctccgtgttc
      181 atctccggca gcattagagc ggggctgtag cacaataccg gagaaccaga tcgggcaggt
      241 aggggattac cgtatatatc ggaccctgtt agccctccaa ttgaggactc cccgatttcc
      301 ctcttcttcc gggtggaaca gcccgacgca gcaagggcaa ccacgacgac agcggctgca
      361 aatacaaatc ccgcccgaag caccagacga ttcactcttt tcatgtcatt tcccctttat
      421 tcttaatggt tattgaaaat aaagtgggga aacaggaaat acggccgcgg ataaatcccg
      481 tccatccact cccccatctc ccacccccag ccgtaccatg atggcgatgg ttggggctga
      541 cccgaccgaa acaactccgt gaataaatac tcccgcatcg gcgcatccac ccgtttgttg
      601 tgtgccatat atagctgccc cgcgcgccag tttttgccgc aacggtagat cgaaagcgtt
      661 gcccgaacgg gcgatgcaac gcggtagata tagtgactgc cgcgggtgac catttcggta
      721 taggaaccag cgcagtgatc cattatctcc ccctcgaaat aaagcgctcg gggggttgtc
      781 aatggctgta tgtcttctgt tcccgcaaag ggaggatcaa agaacgagga aggaaagggc
      841 atccgatact cctccatcag cgccggcgga acgcgggcgg ccagttcatc atgcacggcg
      901 cgcagacgat ccagcgaaac aaattttcgg acatgatgtc ccggcacttt atccatcatg
      961 cgcagggtgt attcaaagaa ggcggatatg ggaggctggg cagcgtcctg cgtatcatcc
     1021 tccgccagat cttccagcag gcgggatgtt atgaactgac ggtaccgagc atccgttgcc
     1081 agacgcagaa tgccggcatt tatccggttc acgtgcgaca gcatttttac aacctctggg
     1141 ctgttgaaac tgtcccggag ataaagcagc gcgttaatgg aaagcgaacg cggcataatc
     1201 tttgacagga tcttccgtgc agtttcagtc gcaggaaagc ccagccacga aagaatgtgt
     1261 ttctgcttcc tgctcataag gcttcgggcg gcgcgaacag gcttctgaac ggcaggctta
     1321 tggaacaccc agttgctggc cagggcatag agtagtgccg gattgctatg gctgagatcc
     1381 atggctcccg gacagcgggc aaacatgttg agcagatgcc acttccgctc gctgtatttt
     1441 aacaactcgt tgcgaatatc cgccggaatc atgtcgaaac agccccccat caccttcata
     1501 tccctgcgcc agttctgcac caagcagtgc tcagtctccg catatattcc cggcaacagg
     1561 cgctgtccta aagggtcggt gtccggaacg tccacgccga ttgctcccgc ccgatacagg
     1621 ttcgttgaca aaagcttatc tgccagtttt cgcgtcgggc gccagttgtg ggatcggcgc
     1681 ttgaaccacg ccctgggatc gggccaaatg gtcatgacta gaacatgccg gggttcaaac
     1741 gagtagagct tcccgttctt gaacgcatag cccctgcggc cctctttgaa atcgctcttc
     1801 atatcattcc tggcaaccta tcaagtcgta ggtccatcgt ccgcatcacc tgttaatcaa
     1861 ccagaacaaa ctcggcccgg cgattcaagc cccacgactg ctcatcatgc ccggatacgt
     1921 ccgggctttc ctcgccgaag ctcttggtat aaatacggct gccgtcaatg ccaaggcccg
     1981 tgagatagga gcgcacagcc agcgcccgcc tttcgccaag cgccatattg tattcccggc
     2041 tcccgcgctc atcgcaatga ccctcgataa tcacaccggt tttgatgttt tgacgcaaac
     2101 tctcagccac gttttcaatc tttgtgcgct ccgaagccca aatctgggaa ctgtcataag
     2161 caaagtatat cgggtcacac agatatgtat actcaacatt gtctcccgga cgctggacat
     2221 tctgcggatt ccatacctct gttccccgag gatcaatgtc tgaccttcgg gatctcgcct
     2281 tacttgaagt acaacctgtt gttgaaataa ttattgcgat ggcagtcagt gggacaatga
     2341 ttcccgtttt tgatgtaatg caacctacaa tattcataat tccttttccc ctgattatcg
     2401 gtattattgc tatctatgtt atactacacg gagaagaatg atattctgcg cgatcgacat
     2461 gtgatttata agccgcagtg aaacacaaga cgcaagctgg gggcaaagac cggggtcgcg
     2521 attatttcac cgcaatgttt cccgtttcag ggtcacaaca tatacacaca tccccccgcg
     2581 gacttcccaa tgacagatgt tttgccaaag ggatttcaat ggattcctga actacccggc
     2641 gaagattgcg cgcaccgaat tcttcactat ttgcgctctt gcacagcgcc gcaagcacgg
     2701 cataatcata atgcaactgg atcttgtgtt tcttgcgaac cgcctgcgcc agcattttga
     2761 gcatattttt agcaatagcc aatatggcat cattccctag cggcgtaaag gttatgatct
     2821 catcaattcg attgataaat tcaacacgaa agtgcttctt cagttcacct ttggccgggt
     2881 tagccgcttc accagctttt tcagatggct ggttgaagcc cagtggagca gcacgcgccc
     2941 cggagcttgc ccataggtta gaagtcatga taaatatagc attgcgagca tcggatgtgc
     3001 gacctttccc gtctgtgatg cgcccttcgt caaaaagctg tagaaatata tccagtactc
     3061 gcggatgggc tttctcgatc tcatccagca gcacgattga ataaggcatc gtgcgcagat
     3121 tccctgg
//
LOCUS       DAOQWR010000206         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_33307_length_3110_cov_5.800982, whole genome shotgun sequence.
ACCESSION   DAOQWR010000206 DAOQWR010000000
VERSION     DAOQWR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33307_length_3110_cov_5.800982"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..2147)
                     /gene="speA"
                     /locus_tag="PKK36_11540"
     CDS             complement(222..2147)
                     /gene="speA"
                     /locus_tag="PKK36_11540"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HNR95227.1"
                     /translation="MEAKSTHEENLAWSSRDAAEWYGIRGWGTGYFDVSPEGEVTIHP
                     QGPSGPSVSLMKIVSGLHERGMEMPVLLRIENILASRIKLLHERFAAAIREFGYRGEY
                     RGVYPIKVNQQEQVVENIVKYGAPYHHGLEAGSKAELIAAMSFLTDPEAFLICNGYKD
                     EEFIDLGLYALKMGIQCVFVLESPGELALILERAKALQVKPILGVRIKLSAQAGGHWA
                     ESGGDRSVFGLNAAQVIDVVDQLRAAGMLDCLKLLHYHLGSQIPNIRDIREAVVEACR
                     YYVGLVKEGAPMGVLDLGGGLAVDYDGTHTVSSSSRNYGLNEYCADIIEVVMGMLSDE
                     EIPHPMIITESGRATVAYYSVLLFDVLDVSSYEKDTDLNPVPEDAPEALINLYETVAG
                     FTPGRMLELYHDGIYYRDEIRQLFKHGAASLRERAQAEEIFLELMEKVVVEMRGMDRI
                     PEELEGLESALSDIYYGNFSVFQSLPDAWAIDQLFPVMPIHRLNENPTQRATLADITC
                     DCDGKLEKFIGDQGIQSVLRVHTLKDGEPYYLGVFLVGAYQETLGDLHNLLGDTNVMS
                     IRIDENGEVLYSRETEGDSVADVLSYVEYETKGMLLRVREKAEQAVSGGRITAQERKQ
                     ILDAYEAGLRGYTYFEA"
     gene            complement(2166..>3110)
                     /locus_tag="PKK36_11545"
     CDS             complement(2166..>3110)
                     /locus_tag="PKK36_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95228.1"
                     /translation="SIMNLDGLIVLSPHLERVAGAAYMLRHLHIGTLYLPPALAAVTP
                     DMTEDEFFALVLEGNLAAEYDEEFLRALYAEIIGTSDMPYRPSLARAMKKRGATFVNK
                     WAGSTVPHRALTAGDVVYEEMHNGKRFAMEVLNPSPAAPAGDFPMENRSLVLRISYGD
                     FAMLVSGDLHFDGQLHLAETVPPEKLAAQVLMAPHHGTAAPPPGFGNPKQQLLDELNR
                     STAVLLDKVRPEKVVFDYGNPGPVYKQRTRDARAAHEITFHFIEERLGAGNVLSTERD
                     LAIFISSNGEGFEIETQGQKVSSGGETAEAVSSLEVGF"
BASE COUNT          591 a          971 c          887 g          661 t
ORIGIN      
        1 tctatctcgc gtttgggcca atatttccat gaaaatcgta gaagaacccg tttttggccg
       61 ttttttggcc ttttttgatg atttttgagc tttgaggaaa ccttctttgc agattcccga
      121 atggagggtt ttgctctgtc aaaaccggat tttctttttc cggaaccgac ccgccttcgc
      181 ccggaggctt cggcgggcag gcgatgcggt tccctccatt ttcaggcttc gaagtaggtg
      241 tagccgcgca ggcctgcttc gtaggcgtcg aggatctgct tgcgttcctg tgcagtgatg
      301 cgcccgccgc tgaccgcctg ttccgccttt tcgcgaaccc ggagcaacat gcccttggtt
      361 tcatattcga cataagacag gacatcggcc accgagtcgc cttcggtttc gcggctgtag
      421 agcacttcgc cgttttcatc aatgcggata ctcataacgt tggtgtcgcc gagcaagttg
      481 tgcagatcgc cgagtgtttc ctgataggcg ccgaccagaa aaacgcccag atagtagggt
      541 tctccgtcct tcagtgtatg aacccggagc acgctctgaa tgccctgatc tccaatgaat
      601 ttctccagct tgccatcgca gtcgcaggtg atgtcggcca gcgtggcgcg ctgcgtgggg
      661 ttctcattca gccggtggat gggcatcacg gggaagagct ggtcaatggc ccaggcatcc
      721 ggcagggact ggaaaacgct gaagttcccg taatagatgt ccgacagcgc ggattcaagt
      781 ccctccagct cctccgggat gcggtccatg ccgcgcattt ccaccaccac cttctccatc
      841 agttccagaa agatttcctc ggcctgggcc cgctcgcgca gggaggccgc gccgtgcttg
      901 aagagctgcc gtatctcgtc ccggtaatag atgccgtcgt gatacagctc cagcattctt
      961 ccgggcgtga agccggcgac ggtttcatac aggttgatca gcgcctcggg cgcatcttcc
     1021 ggaaccgggt tcaggtccgt gtctttttca taggaactga catccagcac atcaaacagc
     1081 agcacggaat aataggccac ggtggcgcgg ccggattccg tgatgatcat cggatgggga
     1141 atctcctcgt cgctgagcat gcccatgacc acctcgatga tgtcggcgca gtactcgttc
     1201 agcccgtaat tgcggctgct ggaggaaacc gtatgggtgc cgtcataatc cacggccaga
     1261 ccgccgccca gatcgaggac gcccatcggc gcgccctcct tgaccagccc gacataatac
     1321 cggcaggcct cgaccaccgc ttcgcggatg tcgcggatgt ttggaatctg cgaacccaga
     1381 tggtagtgca gcagcttgag acagtccagc atgccggcgg cgcgcagctg atccaccaca
     1441 tcaatgacct gcgcggcatt cagcccgaac acactccggt cgccgccgga ctccgcccag
     1501 tggccgccgg cctgcgccga cagcttgatg cggacgccga gaatgggctt aacctgcagg
     1561 gctttcgccc gctccaggat cagcgcgagt tcgccggggc tttccagcac gaacacgcac
     1621 tggatgccca tcttgagcgc gtacaggccg aggtcaatga attcctcgtc cttgtagccg
     1681 ttgcagatca gaaaggcctc agggtcggtc aggaaagaca tggccgcaat cagttccgcc
     1741 ttgcttccgg cctccagccc gtgatggtag ggcgcaccgt atttgacgat gttttccacc
     1801 acctgttcct gctggttgac cttgatcgga tatacgccgc ggtattctcc gcgatagccg
     1861 aattcccgga tggcggcggc aaaccgctcg tgaagaagct tgatgcggga agccagaata
     1921 ttttcgatgc ggagcagcac gggcatttcc atgccgcgct catgcaggcc ggaaacaatt
     1981 ttcatcaggc tgaccgacgg tccggagggc ccctgcggat ggatggtcac ctccccttcg
     2041 ggcgacacgt cgaaatatcc ggtgccccag ccccgaatgc cgtaccactc cgccgcgtcg
     2101 cgggacgacc aggccagatt ctcttcatgc gtgcttttgg cttccaattc cgcgatcctc
     2161 cgttcctaga agcccacctc cagcgaggac accgcctccg ctgtttcgcc tccggaactg
     2221 actttctgtc cctgcgtctc gatctcgaaa ccttccccgt tcgaactgat gaaaatggca
     2281 aggtcgcgtt ccgtgctcag cacattgccg gcgcccagcc gttcctcgat aaagtggaag
     2341 gtgatttcat gggccgcgcg cgcatcacgc gtgcgctgct tgtacaccgg accaggattg
     2401 ccgtagtcga aaacaacctt ctccgggcgg actttgtcca gcagaaccgc cgtggaccgg
     2461 ttcagttcat cgagcagctg ctgtttcgga ttgccgaatc ccggcggcgg cgccgccgtg
     2521 ccatgatgag gcgccatcag cacctgcgcg gccagctttt cgggcggaac cgtttcggcc
     2581 agatgaagct gtccgtcaaa atgcagatct ccggacacca gcatggcgaa atcgccgtac
     2641 gaaatgcgaa gaaccagtga gcggttttcc atcgggaaat ccccggccgg tgcggccggc
     2701 gacggattca ggacctccat ggcaaagcgc ttcccgttat gcatttcctc gtagacaaca
     2761 tcgccggccg tcagcgcgcg atgcgggacc gtgctcccgg cccatttgtt gacaaacgtt
     2821 gcgccccgtt tcttcatggc tcgcgccaga gacgggcggt aaggcatgtc cgacgtgccg
     2881 atgatctccg catacagcgc gcgcaggaat tcctcatcgt attccgccgc cagattcccc
     2941 tccagcacga gggcgaagaa ctcgtcttcc gtcatgtccg gcgtgacggc cgccagcgca
     3001 ggcggaagat agagcgttcc gatgtgcaaa tgcctgagca tgtacgccgc gccggccacg
     3061 cgctccagat ggggcgacag aacaatcagg ccgtccagat tcataatgct
//
LOCUS       DAOQWR010000207         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_33377_length_3105_cov_3.575410, whole genome shotgun sequence.
ACCESSION   DAOQWR010000207 DAOQWR010000000
VERSION     DAOQWR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33377_length_3105_cov_3.575410"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2841)
                     /locus_tag="PKK36_11550"
     CDS             complement(<1..2841)
                     /locus_tag="PKK36_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR95229.1"
                     /translation="MDSYDVGVVEEDPNFPVNYSLQLAGDGTNYESFVWTGPVPASRG
                     FLNFAQNVGGTADAAVIMFDLALLPAEPSTNAFDIVCSIFPNGSADSLSATAYYTLNG
                     GTTNSVPMSLGEDYSYTTSSQIPGQPDGTVLSYWVGVTFGGPGTNSPSFTETNTYNIV
                     ALAGYETFANFDPVNVGAYTDGDFLGQDGSTWTYTQCRGDMLITDETPCLGNNRTPVA
                     AVQSGTISGGCGTLSFDLMKAFSADYNLEVYVNDNLVATLTGGTQSVVDSSGDIVVNA
                     QGDFVLKFIQANSAAGQVSIDNITWTPPDQNAPSLAITNPITSSVSVSNEVTSYMVEG
                     SASTSVVGQISWTNALTGASGTTAAGTSWSQSVALNVGVNVITFSGTNNVGASASDSV
                     TITRQSLPLGTVLMISQYYEGASNDKWIELYNAGESTIDLGAGGYRLGIWANAAREGW
                     KTSVAPSASIVLSNSIAAGDTYLVKHTDSANPTYAVGDIASGSLTPNGDDSVVLYTGE
                     TYDFANVVDAFGLTGSGYADKSYVRANGLTSGVNTDFAPGDWVEFSLAEVEAAGSLAN
                     EYLGYHSPDGGIALSVTITNPAAASLTVSNDVTTYTVQGTASSNVVGQLSWTNALTGA
                     SGAIAAAAEWSISAVALNVGDNLITVSGTNADAVVVSDSVTITRELAPPVIQAASGVA
                     STQFNANWLASIGATGYRLDVATNESFSTGGVGGEALLDEDFSTLTDTAVPDGWSTSG
                     SSDLDYTGAGYYGDAPPAYKFKATGQWLESPSFSAGATGLQFWALGNSGDGSTFTVSG
                     LVNSAWTLIDTVVIASGGATYNVALDSQTTKFKISFTKVVNCGLDDVLVQGSAGGGES
                     SFVPGYQNLDVGNVTTYAVTGLTEGVTYHYRVRAYNDTVTTINSGVTNVTTGASTTPE
                     ITGFSVPAGATATVTLTTTINGETYALEYTTDP"
BASE COUNT          684 a          913 c          835 g          673 t
ORIGIN      
        1 cggatcggtc gtgtattcca gcgcataggt ctcgccgttg atcgtggtcg tcagcgtcac
       61 cgtggccgtg gcgcccgccg gcaccgagaa gcccgtgatt tcaggcgtgg tcgaagcccc
      121 cgtcgtcacg ttggtcacgc cggaattgat cgtcgtcacg gtgtcgttgt acgcacggac
      181 gcggtaatga taggtcacgc cttcggtcag gcccgtcacc gcatacgtcg tcacattgcc
      241 gacatcaagg ttctgatagc ccggcacgaa gctcgattcg ccgccgcctg cactgccctg
      301 cacaagcaca tcatcaagac cgcagttcac aacctttgta aacgatatct tgaacttcgt
      361 tgtttgcgaa tccagagcta cattataggt agctccgccc gaggcgatca ccactgtatc
      421 aataagtgtc cacgcgctgt tcaccaaacc cgagacagtg aatgtgcttc cgtcaccgga
      481 attcccaaga gcccagaact gcaagcccgt cgcacctgcc gagaaagatg gactctcaag
      541 ccactgacct gtcgccttga atttgtacgc aggaggcgca tcgccataat agccggctcc
      601 cgtatagtcc aggtcggaag agccactcgt ggaccaacca tcaggaacgg ccgtatccgt
      661 cagtgttgaa aaatcttcat caagaagagc ttccccgccg acgccgccgg tgctgaacga
      721 ctcgtttgtg gccacgtcca gccggtaacc ggtggcgcca atggacgcca gccagttggc
      781 gttgaactgc gtcgaagcga ctccgctggc ggcctgaatt acaggcgggg ccagttcacg
      841 cgtaatggtg acgctgtcgc tcacgaccac cgcatcagcg ttggttccgc tgacggtgat
      901 caggttgtcg ccgacattca gcgcaacagc ggagatggac cactcggctg cggcggcgat
      961 tgcgccggat gcgccggtga gggcattggt ccatgagagc tgccctacca cattgctgct
     1021 cgccgtgccc tgcaccgtat aggtggtgac atcgttcgac acggtcagcg aggccgcagc
     1081 cgggttcgtg atcgttaccg agagcgcgat gccgccgtcc gggctgtgat accccaggta
     1141 ttcattggcc agagacccgg ccgcctcaac ctcagccagc gagaactcaa cccagtcccc
     1201 aggcgcaaag tccgtattca cgcccgaagt caaaccgttt gcgcggacat agctcttgtc
     1261 agcatatccg cttccggtca gaccaaacgc atcgaccaca ttggcaaagt cataggtttc
     1321 acccgtgtag agcaccacgc tgtcatcccc gtttggcgtg agcgatccgc ttgcgatatc
     1381 tcccacggca taagtcggat tcgcactgtc cgtgtgttta acaaggtaag tgtcccctgc
     1441 cgcgatggag ttgctcaaaa cgatggatgc agatggagca acactcgtct tccagccctc
     1501 acgcgcggcg ttggcccaga tgcccaggcg ataacctccc gcaccaaggt caatagttga
     1561 ttctcctgca ttatagagtt cgatccattt atcgttgctt gcgccctcat agtactgaga
     1621 gatcatcagc acagttccaa gcggcaggga ttgacgcgta atcgtcacgc tgtcgctggc
     1681 ggaggctcca acattatttg tgccggagaa tgtgatcacg ttgacgccca cattcagagc
     1741 aacgctctgg ctccatgagg tcccggccgc agtggtgccg gacgcgccgg tcagagcgtt
     1801 ggtccagctg atctggccga ccacgctggt gctggccgaa ccctcgacca tataagacgt
     1861 gacttcattt gaaaccgaca cggatgatgt gatcgggttg gtgatcgcca gcgacggcgc
     1921 attctgatcc ggcggagtcc aagtgatgtt gtcaatgctc acttgtccag ccgcactgtt
     1981 cgcctgaatg aacttcagga caaaatcacc ctgggcattg accacaatat cacccgagga
     2041 atcaacaaca ctctgcgtac cgcccgtaag ggtggcgacc agattatcgt taacatacac
     2101 ctcaaggttg taatctgcgg aaaaggcctt catgagatcg aaactaagcg ttccgcaacc
     2161 tccggaaatg gttccagact gcaccgcagc aacgggtgtc cgattatttc caaggcaggg
     2221 ggtctcatct gtgatcagca tatccccccg gcactgcgtg taggtccaag tggatccatc
     2281 ctgtccgagg aagtctccgt ccgtataggc tccaacgttc acagggtcaa agttggcaaa
     2341 agtttcatat cctgcaagag caacgatatt atatgtattt gtctctgtaa acgacgggct
     2401 gttggttccc gggccgccga atgttacacc cacccagtag ctgagaacag tcccatccgg
     2461 ctgccccgga atttgcgagc ttgtagtgta gctgtaatcc tcgccaaggc tcatcggcac
     2521 gctgtttgtt gttccgccgt tgagtgtgta gtatgccgtt gcggacaggc tgtccgccga
     2581 accattcggg aagatagagc acacaatgtc aaatgcgtta gttgaaggct cggctggcaa
     2641 cagagccaga tcaaacatga taactgccgc gtctgcggta cctccaacat tctgggcaaa
     2701 gttaaggaac cctctagatg cgggaacagg cccggtccag acaaacgact cgtaattcgt
     2761 tccgtcaccc gccagctgca aggaataatt gacggggaaa ttgggatctt cttccacaac
     2821 tccgacatca tacgaatcca tgccagcggc cggcccatcg actgccgtaa atgttccttc
     2881 ataacttatg aaatcgagta cattggttgt gtcccgatga accaatgcaa tgccatccgg
     2941 gccgccctgt atgccgggag tagaagcgac ggggaaccat gctgctgcgt atccgttgcc
     3001 ttcatcccca agggttccgg aacaattaat ggtatggtat agttaccctc cgtttccatt
     3061 atacagaagt atgtcgtggg tgtataggtt ctttgcggac gctcc
//
LOCUS       DAOQWR010000208         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_33629_length_3090_cov_3.431631, whole genome shotgun sequence.
ACCESSION   DAOQWR010000208 DAOQWR010000000
VERSION     DAOQWR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33629_length_3090_cov_3.431631"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="mutL"
                     /locus_tag="PKK36_11555"
     CDS             complement(<1..598)
                     /gene="mutL"
                     /locus_tag="PKK36_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNR95230.1"
                     /translation="METSGQIRLLSDQVINKIAAGEVVERPASVLKELLENAFDSGAT
                     EITADIIAGGIRAVTVTDNGNGMRPDDAVLSIERHATSKIRDAGDIERIQTMGFRGEA
                     LAAIASVSRMALRTRHAEELSGTEVIVTGGKLQSVAETGVPVGTSVEVRDLFFNTPAR
                     RKFLRSEQTELAHLRQLFITFALAHPAVALRMTVDGKTV"
     gene            complement(661..2130)
                     /locus_tag="PKK36_11560"
     CDS             complement(661..2130)
                     /locus_tag="PKK36_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNR95231.1"
                     /translation="MSLSAHARRILDHIWGPTGSVILHIIIVILLIEFMVFKVQEKQA
                     EIEVFMMDPDAQDLEEFEKELELLEDIPEVQDQISPDVNIEMEAPPDPTQFDSPEPEM
                     DLSALNIMDDVKSPLVMKGLFAGRSASGRAAMLGKHAGRWGAYTEPAVIKALEWLKNH
                     QLENGSWNAQGGSGGGDKPAAMTGLALLAFLAHGETTSSEKYGRTVERAIKYLVSTQK
                     PDGMFCRPRADNSEYIHGIATYAISEAYGLTRIPALKDSMERAVDVIMKGQQPIGSWN
                     YFYEKNARYDTSVTGWQLQALKAAYIAGASNPGLKACTDKSVEYLRKAQFADTGRFAY
                     RSDETKPGRWVAGSNSQAPTAAAVLCMQLLGYGNTTEAKKGSVYLRSATCKWSREETD
                     YYPLYEWYYVTQAKFHEGGQQWSSWNNEFARVLVKEQNEDGSWISPGQNETPYGPVYG
                     TVFCALTLQVYYRFLPTYQTIDVEPVDETDDDDVVVEII"
     gene            complement(2155..2565)
                     /locus_tag="PKK36_11565"
     CDS             complement(2155..2565)
                     /locus_tag="PKK36_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR95232.1"
                     /translation="MARTRKKPESSVSEIPMTPMIDVVFQLLIYFLVTMKPVDVVAHL
                     DVFRPSSQEKQEQSETPPKMIRIAIYADGFTINDKPVRLNDLEGLIFKLASFDKAQTI
                     MIMCTASSPHEKLVQVLDLCAAAGLKNLSVVSTN"
     gene            complement(2574..3011)
                     /locus_tag="PKK36_11570"
     CDS             complement(2574..3011)
                     /locus_tag="PKK36_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNR95233.1"
                     /translation="MRKRRNRSGDAMEVNMTPMIDVVFQMIIFFVCTADMDRKAFDEK
                     IRLAMSPHGPVVEKKDPRTVTIEVDAKGNLQIFRTPYSYGAMQQTLRKAVAEYGQTVP
                     VVIRGDAQTKHEDIKRVMDAAAGAGLWKIKFAATKEVAETVKR"
BASE COUNT          548 a          982 c          897 g          663 t
ORIGIN      
        1 agacggtttt cccgtccacc gtcatgcgca gcgcaactgc tggatgagcc agcgcaaacg
       61 tgatgaacag ctgccgcaga tgcgccagct ccgtctgctc ggagcgcagg aacttgcgcc
      121 gcgccggcgt attgaaaaac agatcgcgca cttcgaccga agttcccacc ggaacgcccg
      181 tttccgccac ggactgcagt ttcccccccg tcacgatgac ttccgtgccg gacagctcct
      241 ccgcatggcg ggtgcgaagg gccatgcgcg aaaccgacgc aatggcggcc agcgcctcgc
      301 cccggaagcc catggtttga atgcgctcaa tgtcgccggc gtcccggatt ttactggtcg
      361 catgccgttc aatcgagagc accgcatcgt ccggccgcat gccgtttccg ttgtccgtca
      421 cagtgacggc cctgatgccg ccggcgatga tgtcggcggt gatttcggtt gctcctgaat
      481 caaacgcgtt ctccagaagc tccttcagaa ccgatgccgg ccgctcgaca acctcgccgg
      541 cggcgatttt gttgatgacc tgatccgaca gcagcctgat ctgaccggaa gtttccatga
      601 gtccgaagcc tagcgcaccg gcggtggcgg cgcaaccgca gaaggcgaat ctctcagcgg
      661 tcagatgatc tcgaccacaa cgtcgtcatc gtcggtctcg tccaccggct cgacatcaat
      721 ggtctggtag gtgggcagga accggtaata gacctgcagg gtcagcgcac agaagacggt
      781 gccgtacacc ggcccgtagg gggtctcgtt ctggccgggg gatatccagc ttccgtcctc
      841 attctgctcc ttgacgagca cacgggcgaa ttcgttgttc cagctgctcc actgctggcc
      901 gccctcgtgg aacttggcct gcgtgacata gtaccactcg tagagcggat aatagtcggt
      961 ttcctcgcgc gaccacttgc aggtggcgct gcgcagatac acactgccct ttttggcctc
     1021 ggtggtattg ccgtagccca ggagctgcat gcacagcacg gcggcggcgg tcggggcctg
     1081 ggaattcgag ccggccaccc agcggccggg cttggtttca tcactgcggt aggcaaaccg
     1141 gccggtgtcg gcgaactggg cttttctgag atactccacc gacttgtcgg tgcaggcctt
     1201 cagccccgga ttggatgctc cggcaatata ggccgccttc agcgcctgca gctgccagcc
     1261 ggtcacggag gtgtcatagc gggcattttt ctcgtagaaa taattccacg aaccgatcgg
     1321 ctgctgcccc ttcatgatga catccacggc ccgctccatc gaatccttca gggccgggat
     1381 gcgcgtgagg ccgtaggcct cgctgatggc ataggtggca atgccgtgaa tgtattccga
     1441 attgtccgcg cggggccggc agaacatccc gtccggtttc tgggtggaca ccagatattt
     1501 gatggcgcgc tcgaccgtcc ggccgtattt ctccgagctg gtcgtttcgc cgtgcgcgag
     1561 gaaggccagc agcgcgaggc ccgtcatggc cgcgggcttg tcgccgccgc ccgagccgcc
     1621 ctgcgcgttc catgagccgt tttccagctg gtggttcttg agccactcca gcgccttgat
     1681 cacggccggc tccgtgtagg cgccccaccg cccggcatgc ttgcccagca tcgcggcgcg
     1741 gccgctggcc gagcggcccg caaacagccc cttcatgaca agcgggctct tgacatcatc
     1801 catgatgttc agcgccgaaa gatccatttc cggctcgggc gaatcgaact gggtcggatc
     1861 cggcggggct tccatctcga tattcacgtc gggcgagatc tggtcctgca cctcgggaat
     1921 atcctccagc agctcgagtt ccttttcgaa ctcttcaagg tcctgcgcgt ccggatccat
     1981 catgaagacc tcaatctcgg cctgcttctc ctgcaccttg aacaccatga attcgatcag
     2041 cagaatgaca atgatgatgt gcaggatcac cgaccccgtg gggccccaga tgtggtccag
     2101 tatccgtctg gcatgggcgc ttaaagacat aacgtcacct ctgtgtattc aaatctaatt
     2161 cgtactcacg accgacaggt ttttgagccc ggcggccgcg cacaagtcca gcacctgcac
     2221 cagcttctcg tgcggggacg aagccgtgca catgatcatg atcgtctgcg ccttgtcaaa
     2281 ggaggccagc ttgaagatca gcccctccag gtcgttcagg cgcaccggct tgtcgttgat
     2341 cgtgaagccg tcggcataga tggcgatgcg gatcatcttg ggcggtgttt ccgactgctc
     2401 ctgcttttcc tgcgaggagg gccggaacac atccagatgg gccaccacgt ccaccggctt
     2461 catggtcaca aggaagtaga tcagcagctg aaagaccacg tcaatcatcg gggtcatcgg
     2521 gatttcgctg acggatgact ccggtttttt tctggtgcgt gccataatgc cacctatctc
     2581 ttcaccgttt ccgcgacttc tttggtcgcg gcgaatttaa ttttccacag gccggcgccg
     2641 gccgcggcat ccatcaccct cttgatgtcc tcgtgcttcg tctgggcgtc accgcggatc
     2701 accaccggca ccgtctgccc gtactccgcc accgccttgc gcagcgtctg ctgcatggcg
     2761 ccgtaggaat agggggtgcg gaagatctgc agattgccct ttgcgtccac ttcgatggtc
     2821 accgtgcgcg gatccttctt ctcgacaacc ggcccgtgcg gagacatggc caggcggatt
     2881 ttctcgtcga aggctttgcg gtccatatcg gcggtgcaga cgaaaaagat gatcatctgg
     2941 aagacgacgt cgatcatggg ggtcatgttg acctccatgg catccccgct tctgtttctt
     3001 ctttttctca taattccatc cctcctgccc gaactgaggc gggagactgc ggactgttat
     3061 tcttccacga cctcgacatt gcgcagggcc
//
LOCUS       DAOQWR010000209         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_35926_length_2949_cov_4.428473, whole genome shotgun sequence.
ACCESSION   DAOQWR010000209 DAOQWR010000000
VERSION     DAOQWR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35926_length_2949_cov_4.428473"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1755)
                     /locus_tag="PKK36_11575"
     CDS             complement(340..1755)
                     /locus_tag="PKK36_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNR95234.1"
                     /translation="MAVKELFNSQVKVVNIGLESFKNALDEAGAPAVQVQWKPPADVS
                     PQAMRVIAAKKAQIEKANKKAMDIILRGMPRVIGLARAIDVIPGMKKNLFLHSGPPIT
                     WARMCGPMRGAMMGGLIYEGMAKTPAEAEKYAKSGKLKFAPCHEHCAVGPMAGVVTPS
                     MPVWIVENKTFGNKAYCTLNEGLGKVLRYGAYGPDVIERLRWMENVLYPVLQRAVKQL
                     GFIDLKNLIAQALHMGDEVHNRNRAGTSLLYRLLAPAIIKSTANASDAAAVLEFIHSN
                     DHFFLNLSMPVCKCVLDAARGIKNSSVCTVMARNGTDFGVQISATGGKWYVGEALVPD
                     ALYFPGFSKADAARDIGDSAITETGGLGGFAIAASPAIVQFVGGVSSDALRYTSTMYE
                     ITAAENNVYQIPSMNFRGTPTGIDVVKVVEKNILPFIDTGVAHKEPGVGQVGAGVLNA
                     PMDPFKKAYEGLAKHLASSRS"
     gene            complement(1758..>2949)
                     /locus_tag="PKK36_11580"
     CDS             complement(1758..>2949)
                     /locus_tag="PKK36_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HNR95235.1"
                     /translation="GQRPPVPVDYDRYAAGEAMKALAGGLHVMLFSDNVSIEDEKELK
                     QFARKKGLLVMGPDCGTAIINGVPLAFANVVNRGDIGVVAASGTGCQEVTSIISNEGC
                     GISQAIGTGGRDVKKDIGGIMFIESLKALAADKDTRVILLVSKPPHETVMREIRKITS
                     KIRKPVVSMFLGAAPTDPLMALTLEEAALKAVALSRGRKVKDVERQLAESDAALRDQA
                     KVLSKRCKKGQKYLRGLFSGGTFCTEAQILYQKLPGGLYANAPAGSAKRLKNLQKSQK
                     NTVIDLGEDEFTVGRPHPMIDFSLRNRRIAQEASDPETAVILLDVVLGYGSNMDPAVE
                     LKNVVAKAAKKVQVICSVTGTDRDPQNRRKVQQVLQQAGAVVMPTNAAACRLAGYIIE
                     NLGA"
BASE COUNT          569 a          916 c          831 g          633 t
ORIGIN      
        1 gccttcctcc ccgtggcaag ccacggggaa cccggcgctt ggctttcttg gaagttttta
       61 agaaaaaagt tccaaccatt ggaacttttg cgcaagcagc tgcccgagag ataaaatgcg
      121 gttcaattga tatatccgcc ggcacagcat aatcctcaag gtgatccatt gccaatcctt
      181 ggtcggcaca gacacgaaac ctgccaatgg aacagatact agtcccagac agaaatcctc
      241 gcaactgaaa aaacagattg ccggaacacg aatattcagg ggcatggacg gaaactctgc
      301 cccgctcgag ggtccgcgtc caaacgcatt tattcctgcc tatgagcggc tggacgccaa
      361 gtgcttcgcg agcccctcgt aggctttttt gaaggggtcc atcggggcgt tcagcacacc
      421 cgcgcccacc tgccccacgc cgggctcctt gtgggccacg ccggtgtcaa tgaagggcag
      481 aatattcttt tcaacgacct tgaccacatc aatgcccgtg ggcgtgccgc ggaagttcat
      541 ggaggggatt tgatagacgt tgttttccgc cgcggtgatt tcatacatgg tgctggtgta
      601 acggagggca tcgctcgaca ccccgccgac aaactgcaca atggccgggg atgcggcaat
      661 ggcaaaaccg ccgaggccgc cggtctcggt gatggcgctg tcgccgatgt cgcgggccgc
      721 atccgccttg gagaagccgg gaaaatacag ggcgtccggc accagcgcct cgcccacata
      781 ccatttcccg ccggtcgcgg atatctgcac gccgaaatca gtcccgttgc gggccatgac
      841 cgtgcatacg ctgctgtttt tgatcccgcg cgcagcatcg agcacgcact tgcatacggg
      901 catggaaaga ttcaggaaga agtggtcgtt gctgtggatg aactccagaa cggctgcggc
      961 gtcggaggca ttggccgttg atttgatgat ggccggagcc agcaggcggt acagcagcga
     1021 ggtgcccgcc cggttgcggt tgtggacttc atcccccatg tgcagggcct gagcaatgag
     1081 attcttgagg tcaatgaaac ccagctgctt cacggcccgc tgaagcacgg gataaagcac
     1141 attttccatc cagcgcagac gctcaatcac atcgggcccg taggcaccat agcggaggac
     1201 tttgcccagg ccctcgttca gtgtgcagta ggctttattg ccgaaagtct tgttttcaac
     1261 gatccacacc ggcattgaag gggtcaccac gccggccatg ggtccgactg cgcagtgctc
     1321 atgacagggc gcaaatttca gcttcccgga tttggcgtat ttttccgctt cggcgggggt
     1381 tttcgccatc ccctcataga tcagtccgcc catcattgcg ccgcgcatgg gtccgcacat
     1441 gcgtgcccag gtgatcggcg ggccggaatg cagaaacagg tttttcttca ttcctggaat
     1501 cacgtcaatg gcccgcgcca gcccgatgac ccggggcatg ccgcgcagaa tgatgtccat
     1561 tgcttttttg ttggccttct ctatctgcgc tttctttgcg gcgatgacgc gcatggcctg
     1621 cggcgacacg tccgccggag gtttccactg aacctggacg gccggagcac ccgcctcgtc
     1681 cagcgcgttc ttgaaggact ccagtccgat attgaccact ttgacttgac tattgaacag
     1741 ttcttttaca gccatgacta tgctcccagg ttttcgatga tgtaaccggc cagacggcag
     1801 gccgccgcat tggtcggcat aacgaccgcg cccgcctgct gcagcacctg ctgaaccttt
     1861 ctgcggttct gcggatctcg gtccgtgccc gtgaccgagc agatgacctg cacttttttt
     1921 gcggccttcg caacgacatt cttaagctcc acggccggat ccatgtttga accgtagccc
     1981 agcaccacat ccagcaggat caccgccgtc tccggatcac tcgcctcctg ggcgatgcgc
     2041 ctgttccgaa gcgagaaatc aatcatcgga tgcggccgtc ccacggtgaa ttcgtcctcg
     2101 ccgagatcaa taaccgtgtt cttctggctc ttctgcagat tcttgagcct tttcgcgctc
     2161 ccggctggag cattcgcgta caggccgccg ggcagcttct gataaaggat ctgcgcctcg
     2221 gtgcagaagg ttccgccgct gaaaagtccg cgcaggtatt tctgcccctt tttacagcgt
     2281 ttggacaaca ccttcgcctg atcgcgaagg gctgcgtcgc tttcggccag ctggcgctcc
     2341 acatccttca ccttgcggcc acgggacagc gcgaccgcct tgagggcggc ctcttccagc
     2401 gtcagcgcca taagcggatc tgtgggtgcg gccccgagga acatcgagac caccggcttc
     2461 ctgattttcg aggtgatttt ccgtatctcg cgcatgacgg tttcatgcgg cggtttcgac
     2521 accagcagaa tgacccgggt gtccttgtct gcggcgagcg ccttgagaga ctcaatgaac
     2581 ataatcccgc cgatgtcctt tttgacatcg cggccgcccg tgccgatcgc ctgcgagata
     2641 ccgcagcctt cgtttgaaat aatagaggtg acttcctggc agcccgtgcc ggaggcggcc
     2701 acgaccccga tatcgccgcg gttcacgaca ttcgcgaacg ccagcggcac gccgttgatg
     2761 atcgcggtcc cgcagtcggg gcccatcaca agcagccctt tcttccgggc gaactgcttc
     2821 agctcctttt cgtcctcaat ggaaacattg tcggagaaga gcatcacatg cagaccgccc
     2881 gcaagggctt tcatggcctc gcccgccgca tagcggtcgt agtccaccgg caccggcggg
     2941 cgctgcccg
//
LOCUS       DAOQWR010000210         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_36325_length_2929_cov_4.220251, whole genome shotgun sequence.
ACCESSION   DAOQWR010000210 DAOQWR010000000
VERSION     DAOQWR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36325_length_2929_cov_4.220251"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKK36_11585"
     CDS             <1..596
                     /locus_tag="PKK36_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNR95236.1"
                     /translation="IAALLSWLLTYYVLREGAPLTVVLTRDPTVDLSILNYIVYIFVI
                     ASSVQFVEMYVRKYFKPLYKAFGVFLPLITTNCAILFACLEIMKHVSGVSDPAQMWGV
                     VESLVMAASGGIGFTIAIVIMAGIREELDHCDVPKVLRGAGITLITAGILALAFMGFT
                     GVDRGLQKVLQGRDKAAESAEAPDASAQTSRNWETGI"
     gene            593..1501
                     /locus_tag="PKK36_11590"
     CDS             593..1501
                     /locus_tag="PKK36_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNR95237.1"
                     /translation="MSIILLAGAIMLVLAILMAWVLGWANKTFHVEVDPRVEAVNAAL
                     PGANCGGCGYVGCAEYAEAVVAGACPPDKCTVGGESCAKELAGILGVSLEQSWPFRPV
                     IHCGAHYSDRLGRTDYKGEQSCTTANLVAGVQGCTYGCLGFGDCVSACDFDAIHIVDG
                     LATVDYEKCVGCGACARICPRNIITMVPFKSRQMVVVTCSNNDMGKDVKAVCKVGCIG
                     CKACERNSGGFFRVDKNLPVLDYDKYDPDNLDSIRLAVDKCPMKRLLFIGWPTEKDLE
                     ATKDMEAPDLVEADFKTTVDQTDWQG"
     gene            1522..1782
                     /locus_tag="PKK36_11595"
     CDS             1522..1782
                     /locus_tag="PKK36_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95238.1"
                     /translation="MDKHIILGVHITDRVKHVAQVQETLTKFGCFIKTRLGLHEVSRE
                     FCAPAGMLVLELLDDDKQFKALKTALAEIEGVEVKEMVFDHP"
     gene            1833..2006
                     /locus_tag="PKK36_11600"
     CDS             1833..2006
                     /locus_tag="PKK36_11600"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNR95239.1"
                     /translation="MDRLFLEASDIETLIEADDCGGMTPYFQQALGVRLLVREEDLDR
                     AKELLASAETSAD"
     gene            complement(2039..>2929)
                     /locus_tag="PKK36_11605"
     CDS             complement(2039..>2929)
                     /locus_tag="PKK36_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95240.1"
                     /translation="DDQPQSRDQDGDMDTNAGRSSDYDYVLTSPALTAKHTNTVVGGT
                     TFANGIVFDTRLSSWGSSLPYPAQSGDSGVEGMQHMAVMKTFQISSQTVAPTSATIYT
                     ISGAGGIISPANPVVSLGASKTITISNSPGYVVSDVMVDGVSIGATGTYTFTSVTNNR
                     SIAATFEAEAGPTNTEFELWMISHGWASDFEAQGAIDHDMDGALTREEYVAGTIPTNP
                     ASRFEIDLEPRISFPSQAVLSWPSATGRLYFIEVKSNVITDTWSPYLSSVPATPPLNA
                     HTGTWNEAERIYFRIEVQKP"
BASE COUNT          571 a          759 c          926 g          673 t
ORIGIN      
        1 ccattgcggc gctgctgagc tggctgttga cgtactatgt gcttagagag ggggcaccgc
       61 tgacggttgt actgaccagg gacccaacgg ttgatttgag catcttgaat tacattgttt
      121 acatcttcgt gatcgcatcc tcggtacagt ttgtcgagat gtatgtccgc aagtacttca
      181 agcctttgta caaggctttt ggagtattcc tcccactgat caccaccaac tgcgcgattc
      241 tgtttgcgtg tcttgagatc atgaaacatg tctccggcgt gagcgatccg gcgcagatgt
      301 ggggcgtggt ggaatcactt gttatggcgg cttcaggcgg catcggtttc accattgcca
      361 ttgttattat ggccggcatt cgcgaagagc tggatcactg cgacgtgccg aaagttctgc
      421 gcggcgcggg cattacgctg atcacggccg gcatccttgc gctggccttc atgggtttta
      481 caggtgttga tcggggcctg cagaaggtcc tgcagggaag ggacaaggcg gctgaaagcg
      541 cggaggcccc ggatgcgtcc gcgcagacca gccggaactg ggagaccggc atatgagcat
      601 tattcttctg gcgggtgcaa tcatgctggt tctggccatc ctgatggcct gggttctggg
      661 ctgggccaac aaaacctttc atgtggaagt ggatccgcgt gtggaggccg tgaatgcggc
      721 tcttcccggc gccaattgcg gcggatgcgg ctatgtcgga tgcgcggaat atgccgaggc
      781 tgttgtggcg ggggcttgcc ctcccgacaa gtgcaccgtg ggcggcgaaa gctgcgcgaa
      841 ggaactggcc ggaatcctgg gggtgtcgct tgagcagtcg tggcccttcc ggccggtgat
      901 tcactgcggt gctcattact cagaccggct tggccgcaca gattataaag gggaacagag
      961 ctgcaccacg gcgaatctgg tggccggtgt gcagggctgc acctacgggt gccttggatt
     1021 tggcgactgc gtcagcgcct gcgattttga cgccatccac attgtggacg gtctggcaac
     1081 cgtggattac gagaagtgcg tgggatgcgg cgcctgtgcg cgcatctgcc cgcgcaacat
     1141 tatcaccatg gtgccgttca agagccggca gatggtggtt gtgacctgct ccaataatga
     1201 catgggcaag gacgtgaagg ccgtgtgcaa ggtcggctgc atcggctgca aggcctgtga
     1261 gcgcaactcc ggaggcttct tccgggtgga taagaatctg ccggtcctgg attatgataa
     1321 atatgatcct gacaatcttg actcgatccg tctggcggtg gacaaatgcc cgatgaagcg
     1381 tctgcttttc atcgggtggc cgacggagaa ggatctggag gccacaaagg acatggaagc
     1441 tccggacctg gttgaggcag atttcaagac caccgtggat caaaccgact ggcagggata
     1501 aactaaccga gggagaacgt tatggacaag catatcattc tgggagtgca tatcaccgac
     1561 cgtgtgaagc atgtggcgca ggtgcaggaa acgctgacca agttcgggtg cttcatcaag
     1621 acccggctgg gcctgcatga agtctccaga gaattctgcg cgccagccgg catgctcgtt
     1681 cttgaactgc tggatgacga caagcagttc aaggcgctga agacagcgct tgcggagatc
     1741 gaaggggtgg aggtcaagga aatggtcttc gaccatccgt gaatccgcct ctggcagttt
     1801 tgcgcaccta tatcagtgag gatgcggcct ccatggaccg gctcttcctt gaggcatctg
     1861 atatcgaaac cctgatcgag gccgatgact gcgggggcat gactccatat ttccagcagg
     1921 cccttggtgt tcgcctgctg gtgcgcgagg aggatttgga tcgggcaaag gagctgctcg
     1981 cgtccgctga aaccagcgcg gattgattcc gcggacccct ctgtcggttt atccctcgtc
     2041 agggtttttg cacttcgata cggaagtaaa ttcgttcagc ttcgttccat gtgcccgtat
     2101 gggcattcag gggcggcgtg gccggaacac tggaaagata cggcgaccat gtgtcggtga
     2161 tcacgttgct cttcacttca atgaagtaca gccttcccgt ggcactgggc catgaaagaa
     2221 cggcctggct gggaaaggat attctcggct caagatcaat ctcgaatcgg gaggccggat
     2281 tggtgggaat tgttccggcg acatattcct cccgggtcag ggctccatcc atatcgtggt
     2341 cgattgcccc ctgcgcctcg aagtcgctgg cccagccgtg agaaatcatc cagagttcga
     2401 attcggtgtt ggtcggcccg gcttcggcct cgaacgtggc ggcaatgctt cggttgttcg
     2461 tgacgcttgt gaacgtatag gtcccggtag ctccaattga aacaccgtca accatcacgt
     2521 cggagaccac atacccgggg ctgttggata tggtgatcgt cttgcttgca ccaaggctta
     2581 cgacgggatt ggccggagaa atgatcccgc cggccccgga aatggtgtag atcgtggcgc
     2641 tggttggggc cactgtttgg gatgaaatct gaaatgtctt catgacggcc atatgctgca
     2701 tgccttcaac tccggagtcg ccgctttgcg ccgggtaggg cagtgaactt ccccatgacg
     2761 aaagacgggt gtcgaatact atcccgtttg caaaggttgt tcccccgaca actgtgttgg
     2821 tatgtttcgc ggtcagggcc gggctggtca gaacatagtc atagtcgctg ctccggccgg
     2881 cgttggtgtc catgtcgcca tcctgatccc tcgactgcgg ctggtcgtc
//
LOCUS       DAOQWR010000211         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_37383_length_2874_cov_4.081234, whole genome shotgun sequence.
ACCESSION   DAOQWR010000211 DAOQWR010000000
VERSION     DAOQWR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37383_length_2874_cov_4.081234"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..2685)
                     /locus_tag="PKK36_11610"
     CDS             complement(349..2685)
                     /locus_tag="PKK36_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95241.1"
                     /translation="MAASNVFNVGSLVDGDARDSYTIVTTLLPLDSLGCGTYTAHVII
                     VDADVDRLDDRLTATNSVTLDMAGNSVPVTNQYITAAGIKLETLAGEVTDGMINTGGW
                     DMAVLLSHPFSPILLDAPDDPQYQVLSAATNWLWSGSWTNGTSVSTNFYATNTVQELD
                     PGNYDLIDTGIYQVVWWAVSEDACDPGMMGTNQMLVVDDDIVPPEWTDPEVFMTNNGV
                     EYSGGWTNVNDFTIVWSEASDADTGFEYRRVLNTDPTTNTAMGVSLGTNNTLHITDAA
                     LGQTTNWIYAVDTDDDRPNDRLKAVNTNFVTRFDNVAPDALYFDSADQGPDASSEIAL
                     SWTALTSAGGQDAYTNPLSPWASYRIFYEIEADPSTNSPYYDVAEYANLGDMETASVI
                     LSNLAFGAEYHLNIAGVDEAGNVGELQWPRTVTLLAFNMTQAVTRVNTTSLVNAAHLS
                     WTATNENGIIQRAVDLIYVDAASFTDVLSNQWQHATTITNDTWAMEDSLNIPSGKTRF
                     YRAAIGNSWMTNRNPRAASKEVWGVRPVTLYPGQNWVALHGQPINNTVIDLIGTNLPA
                     GSGPTSPDATKITWYARTAQSSPPWWVATQQVALVNDGGNYSWAVTEGGATWGNGVAL
                     PLHEAFVIEIPTNRSPQTIYLVGQVPTNTLTQTIQSAASSTTKGRNLISLNLPKAMHP
                     SEMGLLTSGFKGGLAPALSDQLWKYDRANQKAPYAVWYRTTDGTWRMTHPIPFPPVPT
                     NYFSPDDGIVIWIQNSTNNNWNLTQPLPYTEPSIRMNP"
BASE COUNT          595 a          860 c          794 g          625 t
ORIGIN      
        1 ccgccgtcag aatgcccgcg ctggccgagt caaaaccggc ggcattcacg ccgtacagcg
       61 caagcgtctc cttggcccat accaggcaga tcaggagcat gaagcagagg ccctgccaca
      121 aggcaatgta tctcaacggg ccggttttgc gttttctcat cattgtcatg ggcctatcct
      181 tatcaggcgc caaccggcaa cgcaaaccag aaaaacaatc gccccctaca cagggagccc
      241 ctgctgaatg tcttcagtga tgccgcttat gcgccgcctg cgcggatcag ccgctccggc
      301 gggcacaggc ccgccggcgg acaatccgtc agccaacggg tcccggattt acggattcat
      361 gcggatgctc ggctctgtgt acggcagcgg ctgggtcaga ttccagttgt tgttggtgga
      421 attctgtatc catataacta ttccgtcatc cgggctgaaa tagttggtcg gcaccggcgg
      481 gaagggaatc ggatgcgtca tgcgccatgt gccgtccgtg gtccgatacc acacggcata
      541 cggagccttc tggtttgccc ggtcatactt ccacaactga tcagagaggg ctggcgcaag
      601 cccgcccttg aaaccggatg tgagcaggcc catctcggaa ggatgcatgg ccttcggcag
      661 attcaggctg atcaggttgc gacccttggt agtcgaagac gccgcagact gtatcgtctg
      721 tgtcagcgta ttcgtcggga cctgccccac gagataaatc gtctgtggcg atctgttcgt
      781 cggaatctct atgacaaagg cctcatgaag cggcagggcc acgccgttgc cccatgttgc
      841 gccgccctcg gtcaccgccc aggaatagtt gcccccgtca ttcacaaggg caacctgctg
      901 cgtggcaacc caccagggcg gagagctctg ggcggttctg gcgtaccagg ttatcttggt
      961 cgcatcaggt gaagtgggcc cgctgccggc cggcagattc gtcccaataa ggtcgatgac
     1021 cgtattgttg atcggctgtc catgcagcgc gacccagttc tgtccgggat acagagtcac
     1081 cggcctgaca ccccagactt ccttgctcgc ggctcgagga ttgcggttgg tcatccagct
     1141 gtttccgatg gccgcgcggt agaagcgcgt tttgcccgac gggatgttga gactgtcttc
     1201 catcgcccag gtgtcattgg tgatcgtcgt cgcatgctgc cactgattgc tcagcacatc
     1261 cgtgaaactg gccgcatcca catagatcaa gtccaccgcg cgctggataa ttccgttttc
     1321 gttggtggcc gtccatgaca agtgagccgc attcaccagc gaagtggtat tcacacgggt
     1381 caccgcctgc gtcatattaa acgcaagcag agtcaccgtg cgcggccatt gcaactcgcc
     1441 aacgtttccg gcctcatcca cacccgcgat attcagatga tactccgcgc caaacgcgag
     1501 gttcgaaaga atgaccgaag cggtttccat atctccaaga ttcgcatatt ccgcgacatc
     1561 atagtatggc gaattggttg agggatccgc ctcgatttca tagaatatcc gatacgacgc
     1621 ccatggagac agagggttgg tataagcatc ctgtccgccg gcgcttgtaa gcgcggtcca
     1681 cgagagcgca atttccgatg aggcatcagg accctggtcg gcggaatcaa agtacagtgc
     1741 atcaggcgcg acattgtcaa accgcgtgac aaaattcgta ttgacggctt tcagccggtc
     1801 attgggacgg tcatcgtcag tatccaccgc atagatccag ttcgtggtct ggcccagcgc
     1861 ggcatccgtg atatgcagcg tgttgttggt cccaaggctg acccccattg ccgtgttggt
     1921 cgtcgggtca gtattaagaa ccctcctgta ttcaaatccg gtgtcggcat cgctcgcctc
     1981 ggaccataca atcgtgaagt cgttcacgtt cgtccagccc ccgctgtact ccacgccgtt
     2041 gttggtcatg aagacttcag gatccgtcca ctccggtggc acgatatcgt catccaccac
     2101 cagcatctgg ttggtaccca tcatgcccgg atcgcaggca tcttcgctta ccgcccacca
     2161 gaccacctga tatattcctg tgtcaatcag atcatagttg ccgggatcca gttcctgcac
     2221 ggtgttcgtg gcgtagaagt tcgtactgac ggacgttcca ttagtccacg aaccgctcca
     2281 cagccagttg gtggccgccg aaagcacctg atactgcgga tcatccggag catccagaag
     2341 aatcggggag aacggatgcg acagcagcac cgccatgtcc catccgccgg tgttgatcat
     2401 tccgtcagtc acttcacccg ccagtgtctc cagtttgatc cccgctgcag tgatatactg
     2461 attggtcacg ggcacactgt tgccggccat gtcaagcgtt accgaattgg tcgccgtcag
     2521 ccggtcgtcc agacggtcca cgtcggcgtc aactatgatc acatgggctg tgtaggttcc
     2581 gcagcccagc gagtcaaggg gcagcagtgt cgtcacaatg gtgtatgaat ctctggcgtc
     2641 cccatcaacc agtgagccca cgttgaacac attcgaagcc gccacatcac cgtctgagtc
     2701 aagaaccagg atatagggcg gcgccgttta aaacgcaata ccactctgga tatcgttcac
     2761 ccgggcggtc acagcaatgt cgccggcaag cgcctggttt gtatccaggg aggttccgca
     2821 gttccatgtg agatagtcga agaacggcgg attgatatcg tcatccacaa ccgt
//
LOCUS       DAOQWR010000212         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_38284_length_2829_cov_2.715213, whole genome shotgun sequence.
ACCESSION   DAOQWR010000212 DAOQWR010000000
VERSION     DAOQWR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38284_length_2829_cov_2.715213"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKK36_11615"
     CDS             <1..606
                     /locus_tag="PKK36_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95242.1"
                     /translation="VILFNQLTMRVSGKTICCWAFAVVVAYCAVGCSHTQPMVSPAAL
                     RGAGASYRVGISGVYDLNQFDYAPVILDETAKMKVFSDTALTHDLSDTDFDYVIRGNF
                     AYYKVPSKNGFHIFSIYCLWLPLLSGLPNTHIDGRADINFEIYHKGQLLKTYEYNDIF
                     WAERSYLTLWPEPSLEMELRRLARLLLRDMSRDFFGHGVSL"
     gene            603..1235
                     /locus_tag="PKK36_11620"
     CDS             603..1235
                     /locus_tag="PKK36_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95243.1"
                     /translation="MTRCLLVLLLLLVTGCATRLEKAERLMARGEYVKARGYYQQELD
                     IQQNNAHLPQWTGRAYRYSFVPANAAKAILGVGNSYKEQKLLDNAFYHYSYFIQFSLR
                     HNLNFDKEISRIENWILENGPVVSESASGNDQIAVTEEIGPSDMTVMSAETAAQDRNE
                     AAVTAIVESKKNEPAPRAGAKRKPKNSKAVSRKSAKLDHEEENLDMPVSR"
     gene            1117..2073
                     /locus_tag="PKK36_11625"
     CDS             1117..2073
                     /locus_tag="PKK36_11625"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNR95244.1"
                     /translation="MNRRRVPVLKGSRRIAKQFPENPQSWIMKKKILICRYPGNCFWM
                     TMLSVLLLGCATSRVAIQPGLDYQERGEYLRAYQYFKNLTQQHPRRDDLRIHFKTARG
                     LAFRQAVGLAEKALMRDDLDAFLQGMRQARDIQPGEKVDAVVQLVESARLKGRTDAEI
                     REELTGYMREGQKKDLPATLEASAEALGQQVLDGKVHAPVSILGFDAGNGEKEKSSSA
                     YFENALAEALLKKGVKVVERQKMGEIVTEINMSGTGLISPEQMVQAGRLSGAQTIITG
                     NVYLWESAAKYQIRAIDVESSRIVFQETREFLPGETKMLIGK"
     gene            2091..>2829
                     /locus_tag="PKK36_11630"
     CDS             2091..>2829
                     /locus_tag="PKK36_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95245.1"
                     /translation="MNRALCMIAGGLLYSCASTAAGDASAADIPSWWEDPHKHDQSHL
                     YFKAKGESFLSLEDARAEAFKSIKKQVAEYIQSSIEVRDRRIDVASRFELREVESFRE
                     FEPPHRTDQWIVFVLGRYPRTEYERIRGRLEQADRLVKEWAVAQSNVNREEFAQAESR
                     LLGIISQYDLSLSPGFTLEDAKLALAGLYLKQGSVLKARTWIQDVRKSTTEPGWRSRA
                     EDMERNLPEISLNDAFGSRKVALFCCAS"
BASE COUNT          656 a          677 c          830 g          666 t
ORIGIN      
        1 gtaattttgt tcaaccagtt gacgatgcgt gtttcgggca aaaccatctg ttgttgggcg
       61 tttgctgttg tggtggctta ttgtgcggtt ggctgttcac acacccagcc gatggtaagt
      121 cctgccgctt tgcgcggagc cggcgccagt taccgtgttg gaatctccgg ggtttatgac
      181 ctaaaccagt tcgattatgc gcctgtgatt ctggatgaaa ccgcgaagat gaaagtgttt
      241 tcggacacag ccctgacgca tgatttgtca gatactgatt ttgattacgt gatccgcggg
      301 aatttcgcgt attacaaggt gccttcaaaa aacggatttc acatcttttc catttactgc
      361 ctctggctgc ctcttctgtc cggcctcccc aatacgcata ttgacggccg tgcggacatc
      421 aattttgaaa tctatcacaa ggggcagctc ttgaaaacct atgaatacaa cgacatcttc
      481 tgggccgagc gcagctattt gaccctctgg ccagaaccct cgctggagat ggagctgcga
      541 cgccttgcac gccttctgct gcgtgacatg tcccgtgatt tctttggtca tggagtttcg
      601 ctatgacccg ctgtcttctt gttttactgc tgttgctggt caccggctgc gctacccggc
      661 tggagaaggc cgagcggctg atggcccggg gggagtatgt caaagcaagg gggtattatc
      721 agcaggaact ggacatacag cagaataatg cacatttgcc gcagtggact gggagggcgt
      781 acaggtatag ttttgtgcct gcgaatgcgg ccaaggccat cctcggcgtg ggcaacagct
      841 ataaggagca gaaactgctg gataatgcct tctaccatta ttcctacttc atccagttct
      901 cgctgcggca taatcttaat tttgacaagg aaattagccg tatcgaaaac tggattctgg
      961 agaatgggcc tgttgtttcg gaatccgcaa gcggcaacga ccagattgcc gtgacagaag
     1021 aaataggccc atctgacatg actgtgatgt ctgccgaaac ggccgcccag gacagaaatg
     1081 aagccgcagt cacagcgatt gttgaatcca aaaagaatga accggcgccg cgtgccggtg
     1141 ctaaaaggaa gccgaagaat agcaaagcag tttccagaaa atccgcaaag ttggatcatg
     1201 aagaagaaaa tcttgatatg ccggtatcca ggtaactgtt tctggatgac catgttgtcc
     1261 gtcttgctgt tgggctgcgc gacttctcgt gtcgccatac agccgggctt ggactatcag
     1321 gagcgaggag agtatctccg ggcctatcag tattttaaaa acctgacgca gcagcatccg
     1381 cgcagggatg acttgcggat ccatttcaag actgcccgcg gattggcgtt tcgccaggca
     1441 gttgggttgg cggaaaaggc tttgatgcgc gacgatttgg acgcattcct gcagggcatg
     1501 aggcaggccc gcgacataca gcccggcgag aaggtggatg cggttgtcca gcttgttgaa
     1561 agcgcccggc ttaaaggccg taccgatgcc gaaatccgcg aagagctgac ggggtatatg
     1621 cgggagggtc aaaagaaaga tctgcctgcg accctggagg ccagcgcaga agcccttgga
     1681 caacaggtgc tggacgggaa agtccatgca cctgtctcta tcctcggttt tgatgcgggg
     1741 aacggcgaga aggagaaatc atcatcggct tattttgaga atgctttggc ggaagcgctg
     1801 ttgaagaagg gggttaaagt tgttgagcgc cagaagatgg gggagatcgt gacggaaatc
     1861 aacatgtcgg gcactggcct gatcagtccg gagcagatgg ttcaggccgg ccggctgtcc
     1921 ggcgctcaaa ccatcattac ggggaatgta tatctttggg aatctgcggc aaaatatcag
     1981 atccgcgcca ttgacgttga atcaagccgc attgtttttc aggaaacgcg ggagttcctt
     2041 cccggtgaaa caaaaatgct gattgggaaa tgagttcttt gaaaagatgt atgaataggg
     2101 ctctgtgcat gatcgcgggc ggcttgctgt acagctgtgc cagcacggcc gccggggacg
     2161 cttcggcggc tgacattcca tcctggtggg aagatcctca taaacatgat cagtctcacc
     2221 tttatttcaa ggccaagggg gaaagtttcc tttcactgga ggatgcacgc gcagaggcgt
     2281 tcaagagcat aaaaaagcag gttgcggaat atatacagtc ttctatcgaa gtgagagacc
     2341 ggcgtattga cgtcgcaagc cgttttgaac tgagggaagt cgaatccttc agggaatttg
     2401 aaccgcctca ccgcacggat cagtggattg tctttgttct tggccggtat ccgcgcaccg
     2461 aatacgaaag gattcgcggg cggcttgagc aggctgaccg tctcgtgaag gaatgggccg
     2521 tggcccagtc caatgtgaac cgcgaggaat tcgctcaagc cgaaagcagg cttttgggca
     2581 tcatctccca gtatgatctg tcgctttcgc ccggtttcac cttggaggat gcgaaactgg
     2641 cactggccgg actgtacctg aagcagggat ctgttctgaa ggcccgcaca tggattcagg
     2701 atgtgcggaa gtccaccact gaaccgggtt ggcgcagccg tgcggaagac atggagcgca
     2761 atctgccgga aatctcattg aacgacgcct ttggttccag gaaggttgcg ctgttctgct
     2821 gcgcgagtg
//
LOCUS       DAOQWR010000213         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_40515_length_2727_cov_5.199476, whole genome shotgun sequence.
ACCESSION   DAOQWR010000213 DAOQWR010000000
VERSION     DAOQWR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40515_length_2727_cov_5.199476"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKK36_11635"
     CDS             complement(<1..380)
                     /locus_tag="PKK36_11635"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNR95246.1"
                     /translation="MSDFHSKTIHELADLMEKGACTSEQIVRDLLERIEKTDSRICAY
                     LSVNADSALAQARAADSARAGGVRKPLLGIPVAVKELLNVKGQPCTCASKILKGYTAP
                     YTATAMARLEEQGAVLLGRTNMDE"
     gene            complement(390..695)
                     /gene="gatC"
                     /locus_tag="PKK36_11640"
     CDS             complement(390..695)
                     /gene="gatC"
                     /locus_tag="PKK36_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNR95247.1"
                     /translation="MVGQFSENAEHMDVKYIAHLARLYLTDEEAVRFQDQLDHILEHV
                     RKLKEVNVDGVEPMSHAVPVRNVFRKDEVCDGLNRDEVLANAPAQHQHQFRVPKIVE"
     gene            complement(897..1484)
                     /locus_tag="PKK36_11645"
     CDS             complement(897..1484)
                     /locus_tag="PKK36_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNR95248.1"
                     /translation="MQTLEPKTADLDIDANGIVDFILRSDGSTFDILSLADNDILAWT
                     YPPPDYGSQVIQLQAGEAIDGSSSPGWTNVPGLFSSVMESGSIGLWLPPDATGYFGVR
                     LEIEGNAHYGWVYLDNGWAGLGGGDIYGWAYETTPNTSILAGAIPEPTSLLLLGVGSL
                     VVLTRIRKKNREQSASHLRWGRVSPHFVTKQNCCL"
     gene            complement(1602..>2727)
                     /locus_tag="PKK36_11650"
     CDS             complement(1602..>2727)
                     /locus_tag="PKK36_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNR95249.1"
                     /translation="QGGFDTIADRGLAKNVLSIGAVYKIAGGYSGTGSVNIAGFSSYG
                     PSDDGRVKPDVVAAGVGLFTPILYPPDPTNPYYYMTNASGTSFSSPSAAGAVGLLVDL
                     HNRLAPESPYLASTLKGIILHTADDASTRGPDYRTGWGLINADTAAGLVQTDHDAGGK
                     QFIKEVSLLNSNDIEFAVTATGGVPLKVTICWTDPAGAVQPVQLDPTNRVLVNDLDLR
                     IIGPTGATNFPWILNSSNPSNAATTGDNYRDNVEQIVITNAVAGTQYVVRVTHKGTIV
                     DDTGQPAYQDVSIVLAGIFPETRADLAITETVLDGDDELVLWPSIVGQNYRVQTTIDL
                     MNQAWTNLSAEISATKTNTSWESGGAPSGELRFYRLIETN"
BASE COUNT          607 a          753 c          730 g          637 t
ORIGIN      
        1 aactcgtcca tgtttgtgcg gccgagcaga acggcgccct gctcctccag ccgcgccatg
       61 gcggtggctg tgtagggggc ggtgtagccc ttcagaatct ttgaggcgca ggtgcaaggc
      121 tgtcctttca cgttcagcag ttccttgacc gcgacgggaa tgccgagcag cggcttgcgc
      181 acaccgcccg cgcgggcgga atcggccgct cgggcctggg caagagcaga atcggcattg
      241 accgaaaggt aggcgcaaat gcgggagtcc gttttttcga tccgctccag cagatcgcgg
      301 acaatctgtt ccgatgtgca ggcccccttc tccatcaaat ctgccagttc gtgaatcgtc
      361 ttggaatgaa aatcgctcat ggctccccgc tattcgacaa tcttgggcac ccggaactga
      421 tgctggtgct gcgccggggc attggcaagc acctcatccc ggttcagccc gtcacagact
      481 tcgtcttttc tgaaaacgtt ccggacagga accgcgtggg acatcggctc gacaccatca
      541 acattcactt ccttcagttt gcgcacgtgt tcaagaatgt ggtcgagctg atcctgaaaa
      601 cggacagcct cctcgtcagt gagatacagc cgagcgagat gcgcaatgta tttcacatcc
      661 atgtgttcgg cgttttctga aaattgtccg accatatttg tgttccttaa tgaaggactg
      721 actataagag catggaggtt gaacacaaag caaaaattcg ggagttgcag cgtccgctgc
      781 gcattttaca gcgatatatg gtatcctttc caaaaacatc atttcatagg actgacccct
      841 tttctatgac acctttcgtt caggaatatt ccccatgcac atgcgcggtc ctgaattcat
      901 aaacaacaat tctgttttgt tacaaagtgc gggctgaccc taccccaccg taagtgcgaa
      961 gccgattgtt cgcgattctt ttttcggatt cgtgtcagga cgacaagaga gccgacacca
     1021 agcaaaagca ggcttgtggg ttctgggatt gccccagcaa gaattgaggt gtttggtgtg
     1081 gtttcatagg cccagccgta aatgtcacca cctccgagac ccgcccagcc attgtcaaga
     1141 tagacccacc cataatgggc attgccctca atttcaagac gaaccccaaa gtatccagtt
     1201 gcgtcaggag ggagccagag cccaatagac cccgactcca tgactgatga gaaaagccct
     1261 ggcacattcg tccatcccgg agatgaactg ccgtcaatcg cctcgcctgc ctgaagctgg
     1321 ataacttgac tgccgtagtc cggtggcgga tatgtccacg ccagaatatc gttgtcagcc
     1381 agggatagga tgtcgaatgt agacccatcg cttcgcagaa tgaagtcaac gatgccgttg
     1441 gcgtcaatgt cgaggtcggc ggttttcggt tcgagtgtct gcatgtgaat gggctcagcc
     1501 agccatgtca cagatatagc ggccacgccc gtcatggcac accaacacaa cggaaaaacc
     1561 gaaattaaga gaaaccgccg agctctcatt gcgattcctc ctcagtttgt ttctatcagt
     1621 ctgtagaatc gtagttcacc ggacggtgcc ccacccgatt cccacgacgt gtttgtcttc
     1681 gtggcgctga tttctgcgct caaattcgtc caggcttgat tcatcaggtc gatggtcgtc
     1741 tgaacgcgat agttctgtcc tactatggat ggccagagca ctagttcgtc gtcaccgtct
     1801 aaaaccgtct ctgttatagc aaggtccgcc cgcgtctcgg ggaagatgcc cgccaggaca
     1861 attgaaacgt cttgataagc cggttgcccc gtgtcgtcaa caatggtgcc cttgtgagtg
     1921 acgcgaacaa cgtactgtgt cccggccact gcatttgtta tgacaatctg ctcaacgttg
     1981 tcacggtagt tgtcgccagt tgttgcagcg tttgacggat tcgacgagtt cagtatccaa
     2041 ggaaaattcg tggccccggt tggtccaatg attcgcaagt ccaagtcatt aacgagcaca
     2101 cgattcgtgg ggtcaagctg aaccggctga actgcaccgg cgggatcagt ccaacaaatc
     2161 gtgactttca gcggaacgcc acccgttgcc gtgactgcga attcaatgtc gttgctattg
     2221 agaagcgata cttccttgat gaactgcttg ccgccagcat catggtctgt ctgaacgaga
     2281 ccggccgcgg tgtctgcgtt gatgagcccc catcctgtgc gatagtctgg gccgcgtgtt
     2341 gaagcatcat ccgcggtgtg aagaataatg ccttttagtg tagacgccag atacggggat
     2401 tcaggcgcaa ggcggttgtg gagatcaacc aagagcccca cagcaccagc agccgaaggt
     2461 gacgagaagc tggtgcccga tgcgtttgtc atgtagtagt atgggtttgt tggatcgggt
     2521 ggatacagaa tcggggtgaa caggccaaca cccgccgcga caacgtcagg cttcactctg
     2581 ccatcatcgc tggggccgta gctgctgaac cccgcgatgt tcacactgcc agtgccgctg
     2641 taccctcccg caatcttgta gacggccccg atgcttagga cgttcttcgc caatcctcgg
     2701 tcggcgatgg tgtcaaatcc accctga
//
LOCUS       DAOQWR010000214         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_41203_length_2700_cov_4.691871, whole genome shotgun sequence.
ACCESSION   DAOQWR010000214 DAOQWR010000000
VERSION     DAOQWR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41203_length_2700_cov_4.691871"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKK36_11655"
     CDS             complement(<1..358)
                     /locus_tag="PKK36_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95250.1"
                     /translation="MTHILLSIGDRVGEGSYTLHSRFRQAVNFASGSRLAALVDPAIA
                     AGPVNVIIDGLDLPSVCTLEVKGVSVVVNGIPFAVAPAQKYDSSMSFCEGRKIPRQNI
                     RHLRTLLCEHAPEKSMA"
     gene            complement(363..1709)
                     /gene="gdhA"
                     /locus_tag="PKK36_11660"
     CDS             complement(363..1709)
                     /gene="gdhA"
                     /locus_tag="PKK36_11660"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNR95251.1"
                     /translation="MASNYIERIIQHVSRRNKEEPEFCQAVEEVVQAIKPLLERNKIY
                     EEKCILEHMVEPERTIMFRVPWFDDKGVHRVNRGYRVQFNGALGPYKGGLRFHSSVNL
                     SIIMFLGFEQIFKNALTGLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMMELQKHIGEH
                     TDVPAGDIGVGGREIGYLYGMYKRITTRHVGVLTGKGYGWGGSLIRPEATGYGCVYFA
                     EEMLRVRDRDFKNMVCTVSGSGNVSQYCVEKLIQRGAKVVTMSDSNGTVYDSAGIDAE
                     KLAWIMELKNVRRGRISEYAAKFKGVEYRENARPWDVKCDCAFPCATQNEINAADAKK
                     LVGNGCVLVSEGANMPTNPEGIEVFQANKLMYGPGKAANAGGVACSGLEMSQNAMMYS
                     WTAEEVDRRLHDIMVSIHKSCFETAREYDCPENYVVGANISAFRKVADAMISLGHI"
     gene            1874..>2700
                     /locus_tag="PKK36_11665"
     CDS             1874..>2700
                     /locus_tag="PKK36_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HNR95252.1"
                     /translation="MKSKVSRRASSCLADGGKIVQPGKIVPFLEAVLKSGSRVCLEGD
                     NQKQADFLAKALTQVNPEKVKRLHMVQSSLVLPEHLDVFEKGIAQKLDFAYSGPQAKR
                     LYQMVGDGKVKIGSIHTYLELYSRYFSDLNPQVSLVVADYADAAGNLYTGYNTEDTPL
                     ICEATAFNRGIVIAQVKEIREKLPRVDIPGDWVDFIVPTGKDYYIQPLFTKDPAKISE
                     MQILLAMMVIKGIYEKYRITSLNHGVGYNAAAIELLLPKLAGHLKGKICQYWALNPHP
                     "
BASE COUNT          583 a          819 c          710 g          588 t
ORIGIN      
        1 aagccatgct tttttcgggc gcgtgttcgc acagcagggt cctgagatga cggatattct
       61 gccgcgggat tttccgcccc tcgcagaagc tcatcgagga gtcatacttc tgcgcaggag
      121 caacagcgaa ggggattccg ttgacgacaa cggaaacccc tttcacttca agcgtgcaga
      181 cggaaggcaa atccagtccg tcaatgatga cattcaccgg tccggccgcg atggcggggt
      241 caacgagcgc ggccagccgg ctaccgcttg caaaattcac agcctgccgg aaacgggaat
      301 gcagggtgta ggatccttcc cccaccctgt ccccgatgct gagcaggatg tgcgtcacgg
      361 catcagatat ggcccagcga aatcatcgca tcggccactt tgcggaatgc cgaaatgttg
      421 gccccaacga cgtagttttc aggacagtcg tattcacggg ccgtctcgaa gcatgattta
      481 tggatgctga ccatgatatc atgcaggcgg cggtccacct cctctgccgt ccaggaatac
      541 atcatcgcat tctgactcat ttccagtccc gaacatgcaa cgccgccggc attggccgcc
      601 ttgcccggcc cgtacatcag cttgttcgcc tggaagactt caatgccctc ggggttcgtg
      661 ggcatattgg cgccctcgct gaccagcacg cagccgtttc caaccagctt ctttgcatcc
      721 gcggcattga tctcattctg cgtggcacag gggaaggcgc agtcgcactt cacgtcccat
      781 ggccgggcat tctcgcgata ctcaacaccc ttgaactttg ctgcatactc gctgatgcgg
      841 ccgcggcgga cattcttcag ctccatgatc caggccagct tctcggcatc aatcccggcg
      901 gagtcataaa ccgttccgtt cgagtcggac atggtcacca ctttcgcgcc gcgctgaatc
      961 agtttttcaa cgcagtactg ggaaacattc ccggaacccg acacggtgca gaccatgttt
     1021 ttgaaatcac ggtcacggac ccggagcatt tcctccgcga agtacacgca cccgtaaccg
     1081 gtcgcctcgg gacggatcag ggatcctccc cagccgtatc ccttgccggt caggacacca
     1141 acatggcgcg tggtgatccg cttgtacatt ccgtagagat agccgatttc acgtccgccc
     1201 acgccgatgt cgccggccgg gacatccgtg tgctcgccga tgtgcttctg cagttccatc
     1261 atgaaggact gacagaaacg catcacttcg ttgtctgatt tgcccttcgg gtcgaaatcc
     1321 gagccgccct tgccgccgcc catgggcagg ccggtcagcg cgtttttgaa tatctgttca
     1381 aacccgagaa acatgataat gctcaggttg acggaagaat ggaagcgaag accgcccttg
     1441 tacggcccga gcgctccgtt gaactggaca cggtatccgc ggttcacgcg gtgcacgccc
     1501 ttgtcgtcaa accacggcac acggaacatg atggtccgct ccggctcgac catgtgctcc
     1561 agaatgcact tctcttcgta tatcttgttg cgctccagca gcggtttgat ggcctgcacc
     1621 acctcttcga ccgcctggca gaattccggt tcctccttgt tgcgccggct cacatgctgg
     1681 ataattctct caatataatt tgatgccata ttgaccctct gtttttccct ttgcagttca
     1741 catgaactgc tcttgtgcgg gcagaaaatg aaggaattat aaccgcgaat caagcacctt
     1801 tgttcaattt cttgccttcg gaggcgaaat ctggaaataa tgccgcattc cattcaatga
     1861 aagcgttgca gatatgaaaa gcaaagtttc gcgccgtgca tcctcctgcc tggcagacgg
     1921 ggggaaaatc gtccagcccg gaaagatcgt cccgtttctt gaagccgtgc tgaagtccgg
     1981 aagccgagtg tgccttgaag gcgacaacca gaagcaggcc gactttctag cgaaggccct
     2041 cacacaggtg aaccccgaga aagtcaagcg tctgcacatg gtgcagtcct cgctggtgct
     2101 tcccgagcat ctcgacgttt ttgagaaggg catcgcacag aaactcgatt tcgcgtactc
     2161 tggtccgcag gccaaacggc tctatcagat ggtgggcgac ggcaaggtca agatcggatc
     2221 gattcatacc tacctcgaac tctattcgcg ctatttctcc gatttgaatc cgcaggtttc
     2281 actggtggtg gccgactatg ccgacgccgc cggcaatctc tacacgggct acaacacaga
     2341 agacaccccg ctcatctgcg aggccacggc cttcaaccgc gggatcgtca tcgcgcaggt
     2401 gaaagaaatc cgcgaaaaac ttccccgcgt ggatattcca ggggactggg tggatttcat
     2461 cgtgcccacc gggaaggact actacatcca gccgctgttc acgaaggatc ccgcgaagat
     2521 ttctgaaatg cagattctgc tggcgatgat ggtcatcaag gggatctacg agaagtacag
     2581 aatcacctcc ctgaaccacg gcgtcggcta caacgccgcg gccattgaac tgctgcttcc
     2641 gaaacttgcc gggcacctca agggcaagat ctgtcagtac tgggcgctga acccccaccc
//
LOCUS       DAOQWR010000215         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_42984_length_2623_cov_3.728193, whole genome shotgun sequence.
ACCESSION   DAOQWR010000215 DAOQWR010000000
VERSION     DAOQWR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42984_length_2623_cov_3.728193"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKK36_11670"
     CDS             complement(<1..683)
                     /locus_tag="PKK36_11670"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNR95253.1"
                     /translation="MSASWYLHRLRAMSLEEICHRARFAARKRHWRRGPDASWRMDEA
                     PFPAEGKIVFQNLPAAATEALRAEAKRYLDHRWLFFGLDIKEEPINWHADPVSGKQAP
                     QIFGFDINHRDASAIGDVKLIWEKNRHHHLSVLAAAYALTGDERFSDEVERQILSWIG
                     QNPLFSGVNWTHPLEQGIRLISWVWCERLLRDSNAHSRLFAAGSPVLQSIYQQQRFIA
                     ETYCRGSSA"
     gene            complement(701..>2623)
                     /locus_tag="PKK36_11675"
     CDS             complement(701..>2623)
                     /locus_tag="PKK36_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNR95254.1"
                     /translation="DKVMNKLDSLTPLGYSAAGTVTAVGAGVNDLTVGQAVACAGAGY
                     ANHAEVNFVPRNLVVPVPKDVRMEHAAFSTIGAIAMQGFRQGEMRLGETACVIGLGLL
                     GQILVQILNAAGVRVLGIDLSEERCELARKLGALNACRPDDPGLKSSVRQITRGVGCD
                     AVFICAAGDSKQPAELAVELSRDRGRVIDIGKTHLDLPWKDYYEKEMEVKFSRSYGPG
                     RYDPNYEERGVDYPIGYVRWTENRNMQAFLDLVASGRMQLDPIISNVYPFSEAESVFG
                     QVGDGTIRGLGFVFKYDDAPAEKPASIPSAKPAKAGPVRLGVIGAGNYASSMLLPHLA
                     DNPSVEMRHVVTTTSLTAQNAARKFGFQNSGTDVSAVLNDTDTNAVLIATRHASHAKL
                     TAQALRAGKAVFVEKPLAIDLAGVEEIRAAIVESGNERLQVGFNRRFSPVIRAMKEHI
                     RGIKTPLVLNYRVHAGQTDAKSWYADASEGTRFAGEAGHFLDVFAFLTGARPFSVTAS
                     ALSPDNASADDRENIIATVEYDNGSVASLLYLTQGSPKVPKECLEVFGGGMTMQMINF
                     ESLHIFSGLKKKTVKGGMDKGQKNEMKAFVDAVETGGPMPISVECLLDTTLVTLAALD
                     SVRRGEKIMLADCWQS"
BASE COUNT          575 a          804 c          701 g          543 t
ORIGIN      
        1 ttggcggatg atccccggca gtaagtttcc gcaatgaatc tctgctgctg ataaatggac
       61 tgcagaaccg gtgacccggc cgcgaataaa cgactgtggg cattggaatc gcgaagaagt
      121 cgctcgcacc agacccagga tatcagccga atgccctgct ccagcggatg agtccagttg
      181 acccctgaaa acagcggatt ctgcccgatc cacgacagaa tctgccgctc aacctcatca
      241 ctgaagcgtt catcccctgt cagcgcatat gctgccgcca ataccgacag atgatgatga
      301 cgattcttct cccagatcaa cttgacatcg ccgatggccg aggcatcacg gtgattgatg
      361 tcaaaaccaa atatctgcgg ggcctgtttt ccggataccg ggtcggcatg ccagttgatg
      421 ggttcctctt ttatatccag tccgaaaaaa agccagcgat ggtccagata tctttttgcc
      481 tccgcccgca aggcttccgt cgcggcggcc ggcaggttct gaaaaacaat cttcccttcg
      541 gcgggaaatg gcgcttcgtc catccgccat gatgcatcgg ggccgcgccg ccagtgccgt
      601 tttcgggcgg caaaacgggc gcgatggcag atttcctcca ggctcatggc ccgaagacga
      661 tgtaaatacc atgaagcgga catgatcttt ttgcagcgaa tcaggactgc cagcagtcgg
      721 ccagcatgat cttttcaccc cttctgacgg aatccagcgc ggcaagcgtc accagggtcg
      781 tgtccagcag gcactcgacc gagataggca tcgggccacc ggtctccacg gcatccacga
      841 aagccttcat ttcatttttc tggcccttgt ccatgccgcc tttcaccgtt ttcttcttca
      901 ggccgctgaa aatgtgcagc gactcgaagt taatcatctg catggtcatc ccgccgccaa
      961 acacctccag gcactccttg ggaactttgg gcgagccctg cgtcagatag agaagactgg
     1021 ccacagatcc gttgtcatat tcgacggtgg caatgatgtt ctcacggtca tcggccgagg
     1081 cgttgtccgg cgacaacgct gatgccgtca cggagaacgg cctcgctcca gtgagaaacg
     1141 caaacacatc gagaaaatgt ccggcctcgc ccgcaaaacg cgtgccctcg ctcgcatcgg
     1201 cataccacga tttcgcgtcg gtctggcctg catgcacccg atagttcagc acaagcggcg
     1261 ttttgatccc gcggatatgc tctttcatcg cccgaatgac gggagaaaac cggcggttga
     1321 atccaacctg aagacgttca tttccgcttt ccacaatggc cgcgcgaatc tcctcgaccc
     1381 cggccaaatc aatggccagc ggcttctcaa caaataccgc tttcccggcg cgaagcgcct
     1441 gagccgtcag ctttgcatgc gaggcgtgcc gcgtggcgat cagcacggca tttgtgtccg
     1501 tgtcattgag aacagccgaa acatccgttc cgctgttctg gaatccgaac tttcgcgcgg
     1561 cattttgcgc tgtcaggctg gttgtggtaa ccacatggcg catttcaacg gagggattgt
     1621 ccgcaaggtg cggaagaagc atgctggaag catagtttcc cgcgccgatg acgcccagcc
     1681 tgaccggccc ggctttcgca ggttttgcgg aagggataga tgcgggcttt tcagcgggag
     1741 catcatcata tttgaatacg aagcccaggc cgcgaattgt accgtcgccc acctgtccga
     1801 aaacggattc cgcctcgctg aacggataaa cattcgaaat aatcggatca agctgcatgc
     1861 ggccggacgc gaccagatcg agaaacgcct gcatatttct gttttcggtc cagcggacat
     1921 agccgatcgg ataatccacg ccgcgttctt catagttcgg atcataacgg ccaggaccgt
     1981 aggaacggga gaacttaacc tccatttcct tttcataata atccttccaa ggaagatcaa
     2041 gatgggtttt cccaatatca atcacacggc cgcgatcgcg tgaaagctcg accgccagtt
     2101 ccgcaggctg tttgctgtcg cccgcggcgc agatgaaaac agcatcgcat ccaacccccc
     2161 tggtgatctg ccgcaccgag gatttcaatc cgggatcatc gggccggcag gcgttcagcg
     2221 cacccagttt tcgggccagt tcgcagcgct cttccgagag gtcaatgccc agcacgcgca
     2281 cgccggcggc attgagaatc tgaacaagaa tctgacccag aagcccaagt ccgatcacgc
     2341 aggcggtttc cccgaggcgc atttccccct gccggaatcc ctgcatggca atcgcgccaa
     2401 tggtggaaaa cgctgcatgc tccatgcgga catccttggg caccggcacc acgagattgc
     2461 ggggcacgaa attaacttca gcatgattcg catagccggc gcccgcgcag gcgaccgcct
     2521 gtccgacggt caggtcgttt acgccggcac cgaccgccgt caccgtcccg gcagcggaat
     2581 agcccagcgg agtcagcgaa tcgagcttgt tcatgacctt gtc
//
LOCUS       DAOQWR010000216         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_43380_length_2608_cov_4.116334, whole genome shotgun sequence.
ACCESSION   DAOQWR010000216 DAOQWR010000000
VERSION     DAOQWR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43380_length_2608_cov_4.116334"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PKK36_11680"
     CDS             complement(<1..992)
                     /locus_tag="PKK36_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95255.1"
                     /translation="MLKKISIAGSRYRGELLFMAANYTTPLVAFYASSIAAFFITPEE
                     MGSVQAALLIIPYFSMLHLGVFHGLNRNLAFYFGQGNREKGLMMVNASAFIARTTAWI
                     GALAGLGLVACALLKQNSNMITVAAGVAAALALYCTPYITHISTTNRSGQHFHAYGKI
                     VFISNILRLVYSSLPVIAGWIGYVAALALQPLITVGLLKAKEPYPAEKRFQWADLGEL
                     IKVGFPIMVVGYFTNLLMVADQTFIALYMPKERLGHYVLAKLIMQAMIIIPATLSILI
                     SPKVASCYGETGSARSLRKYFWAFFWLHVAFIVPVCALAYVAVGPLVHWLLPRY"
     gene            complement(986..2344)
                     /locus_tag="PKK36_11685"
     CDS             complement(986..2344)
                     /locus_tag="PKK36_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95256.1"
                     /translation="MNKRLHIWREWVRAYWGRMEEYPELFPLLCRMYFFDYYGGRNME
                     VAAHPEPAAEPPPATGCSACGIEGDVLFVAQSARENCLGVIRRLIQQLQIEAPGIRSI
                     IFQTRRNVVVPPAAENLPPVITYQERVCFPCIARLLRLMGVIWLEGMKVPRVRKCICR
                     HPVEVMLHAVKILIRFPQIQKLLADAKVKMVVAPNEVTAEASLLFPVARRMGIKTVQY
                     LHGTPNRLFVPFISDEFWVWSEVAGQMLTGDRRDDRVVPIGSLEHSDCAVVRSDNTPA
                     GNSSGPRILFLSQIKGDQGWQISAFGRAAERVARIVSAAADTELRVRQHPHEGDEEHR
                     RLEEIFKDTHWQRSSCATSLDKDIAWATHIYTASSTGILAGLAAGKPCYLLWDDELDD
                     IHRRPFFPEDYVVRTDEQMRRSLSRAWSASESQSILTGISGTPGSLQRAVQRIKQIAG
                     SC"
     gene            complement(2408..>2608)
                     /locus_tag="PKK36_11690"
     CDS             complement(2408..>2608)
                     /locus_tag="PKK36_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR95257.1"
                     /translation="IEKMVNAGIYVLNPDVIRHIPAEYYPITEVANSLLRSGRRVGAF
                     DLLNEWIDIGHHDQLRRARQGY"
BASE COUNT          618 a          767 c          689 g          534 t
ORIGIN      
        1 atgtaccggg gcagaagcca gtgcaccagg ggcccgacag caacataggc cagcgcgcag
       61 accggcacaa taaaggccac atgcagccag aaaaaagccc agaaatactt gcgaagcgat
      121 cgtgcgcttc ccgtttcccc atagcagctc gccaccttgg gcgatatcag tatgctcaat
      181 gtcgcgggaa ttataatcat ggcctgcatg atgagctttg ccagcacata atgtccaagc
      241 ctctccttgg gcatgtacag ggcaatgaac gtctggtcag cgaccatcag caggttggtg
      301 aaataaccca ccaccatgat agggaagccc accttgatga gctctccaag atcggcccat
      361 tggaacctct tttccgcagg ataaggctct ttggccttca agagacccac cgtgatcaga
      421 ggctgcagcg ccaaagcggc cacatacccg atccatcccg caatcacggg cagcgacgaa
      481 tagaccagtc tcaggatatt ggatatgaaa acgattttgc cataggcgtg gaaatgctgg
      541 ccgctgcggt ttgtggtgct gatatgcgtg atgtatggcg tgcaataaag cgccagcgcc
      601 gccgcgacac ccgccgccac ggtaatcata ttggaattct gcttcagaag cgcgcaggcc
      661 accagaccca gccccgccag cgcaccaatc caggctgttg ttcgggcaat gaatgcggaa
      721 gcattcacca tcatcagtcc cttttcccgg tttccctggc cgaaatagaa cgccagattg
      781 cggttcaacc cgtggaagac gccaagatga agcatgctga aataggggat gatcagcagc
      841 gccgcctgca ccgaccccat ttcctccggc gtgatgaaaa acgccgcaat ggaactggca
      901 taaaaagcaa ccagcggtgt ggtatagttc gccgccatga aaagaagctc ccctctgtag
      961 cggctgcccg caatggagat ttttttcagc atgatccggc aatctgtttt attcgctgca
     1021 ccgctctctg aagtgatccg ggggttcccg agatcccggt caggatcgat tgagattcag
     1081 aggcgctcca tgcgcgggac aggctcctgc gcatttgctc gtctgtgcga acaacatagt
     1141 cttcggggaa aaagggccgg cggtgaatgt catccagctc atcgtcccac agcagataac
     1201 agggcttccc ggccgcaaga cccgccagaa tgccggtgga gctggcggta taaatatggg
     1261 tggcccacgc gatatcttta tccagcgaag tcgcacaaga tgaccgctgc cagtgtgtgt
     1321 ccttgaatat ctcttccagc cggcgatgtt cctcatcgcc ctcgtgagga tgctggcgca
     1381 cgcgaagctc tgtatccgct gcggcagaaa cgatcctcgc caccctttcc gccgcacggc
     1441 caaacgccga tatctgccag ccctgatccc ccttgatctg ggacagaaac aaaatccggg
     1501 ggcccgacga gttcccggcg ggagtgttat cactgcgcac caccgcgcaa tcgctgtgct
     1561 caagcgatcc gattgggaca acgcggtcat cacgacggtc gccggtcagc atttggcccg
     1621 cgacttcact ccacacccag aattcatcac tgataaaggg gacaaaaagc cggttcgggg
     1681 tgccatgaag atactgaacc gttttgattc ccattctgcg cgcgacagga aacagcagag
     1741 acgcctctgc cgtcacctcg ttgggcgcaa ccaccatttt gacttttgcg tcggccagaa
     1801 gtttttgaat ctgcggaaac cgaatcagaa ttttcaccgc atgcagcatc acttcaacgg
     1861 ggtgccggca aatgcatttc ctgacccggg ggaccttcat cccttccagc caaatcacac
     1921 ccatcagccg cagcagcctg gcaatgcaag ggaaacatac cctttcctga taggtaataa
     1981 cgggcggaag attctccgcg gcgggaggaa ccacaacatt cctgcgggtt tgaaatatga
     2041 tgcttcgaat accgggggct tcaatctgca actgttgaat gagcctccgg atcactccca
     2101 aacagttctc ccgggccgat tgtgcaacaa aaagcacatc accctcgatt ccgcatgcgg
     2161 aacagccggt ggcggggggc ggctccgcgg ccggctccgg atgcgcggca acttccatgt
     2221 ttctgccgcc gtagtaatcg aagaaataca ttctgcagag cagaggaaag agttccggat
     2281 attcctccat gcggccccag taggcccgca cccattcgcg ccagatgtgg agtcttttgt
     2341 tcatgcggca gacaagatat gactgaagag accggatttt ctccggactt cgaaatagaa
     2401 gctttttcta gtatccctga cgggcccgtc tcagctggtc gtgatggccg atatcaatcc
     2461 attcattcaa aaggtcaaac gcgcccacac ggcggccgga gcgcagcagg ctgttcgcca
     2521 cttcggtaat tggatagtat tctgccggga tatgcctgat gacatcggga ttcagcacgt
     2581 aaattcccgc attcaccatc ttttcgat
//
LOCUS       DAOQWR010000217         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_43692_length_2596_cov_5.546242, whole genome shotgun sequence.
ACCESSION   DAOQWR010000217 DAOQWR010000000
VERSION     DAOQWR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43692_length_2596_cov_5.546242"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKK36_11695"
     CDS             <1..486
                     /locus_tag="PKK36_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95258.1"
                     /translation="VPGLGRKTFERLTGQKVSSDATRYAELIHEILGPPKHGIPSVRD
                     VVRRFTLLEDFDGCIITHSDGYTMASSWDHPKEEALGAFTPQMFKKVAQYISQLDMGA
                     LSSITFTIEQQPITIICSGSVFLIAIHVPSRFNKRSVSIAQAIGAEIGRRLTSARDRE
                     E"
     gene            584..1525
                     /locus_tag="PKK36_11700"
     CDS             584..1525
                     /locus_tag="PKK36_11700"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNR95259.1"
                     /translation="MIFLFQIVCVLALVSCGYFARRGGLITAQGTTDLARICIAIIYP
                     ALIFVSMTQLSLAELRANYSLPLLAMVIAISGFLLGLLAARILGDVHPATTRAFLFHC
                     LINNYLFLPLPLVLFRFGEKGVALLVFSSVGYEVLLWSLGVVLFTTDLSGWQRIKSLL
                     SPPFVTLITALLFVVLRDLFTWPAPPGWLALAWQTVRSGMHAVGQATIAFSMLVAGSR
                     FAVMKHEVMLGWRVWLVSFIRLVAVPLIMIPVLGLIPMDETARGLLTIVAVMPAAMAS
                     VLFSERFGGDTDFIAGGLLLTHLWALVTVPLFLAWAL"
     gene            1625..>2596
                     /locus_tag="PKK36_11705"
     CDS             1625..>2596
                     /locus_tag="PKK36_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNR95260.1"
                     /translation="MRLKSLLVPVLAAGLLTCAARAEVILQFFNNTWNEIADEIPEIA
                     EAGYTALWLPPPQKASGGLSVGYDLWDPFDLGSVDQRSTVKTRYGTEEDLLRLIETAH
                     RFGLRVYFDNIMNHRAFDIPGYNENTPVDIYPGMLPEDFHLRVTEEGFYRKWDNTVNW
                     GSTWEVQNRNLSDLIDIAHETPNANFGPNEGDTHPKISFVRHPDNPEFYDYHPTLGWV
                     GFGTTNITTNTLAEHEDYYEEDVGGYLMRSIRWLVDKTKVDGLRLDAVKHVPGYFFGE
                     QWDAGKDSSDSGYCGQAQWQFNMSRGFSDWDNHRDTVFDTEKSYGRND"
BASE COUNT          494 a          771 c          756 g          575 t
ORIGIN      
        1 gtgcccggtc tgggccgaaa aacctttgag cggctcacgg gccagaaagt cagcagtgac
       61 gccacccgct acgccgaact tattcacgag atcctcggtc cgcccaagca cggcatcccc
      121 agcgttcggg atgttgtccg gcgcttcacg ctgctggagg atttcgatgg atgcatcatc
      181 acgcattccg acggctacac catggcctcc agctgggacc atccgaaaga ggaggcgctc
      241 ggggccttca cgccgcagat gttcaagaag gtggcccagt atatcagcca gctcgacatg
      301 ggggcgttgt cctccatcac cttcaccatc gaacagcagc cgatcaccat catctgcagc
      361 ggaagcgtgt tcctgatcgc gatccacgtg cccagccgct tcaacaagcg gtcggtcagc
      421 atcgcgcagg ccattggcgc cgaaatcggc cgccgcctga catcagcgag ggacagggaa
      481 gagtgacgga ccgccgcgtt ttttccaatg attggaactt tttccggaaa aagttccaat
      541 cattggaagt tttcacgcaa aaagttccaa tcattggaag tttatgatat ttctgttcca
      601 gattgtctgt gttttggccc ttgtgtcctg cggatatttt gcgcgccgcg gcgggctgat
      661 caccgcgcag ggcacgaccg atctggcgcg catctgcatc gcaatcatct atcccgcgct
      721 gatctttgtt tctatgaccc agctttcgct ggccgaactg cgcgccaatt attcgcttcc
      781 gcttcttgcc atggtcatcg cgatttcggg gtttcttctg ggactgctgg ccgcgcgaat
      841 tcttggggat gtgcatccgg ccaccacgcg cgcgtttctg ttccactgcc tgatcaataa
      901 ttatcttttt cttcccctgc cgctggtgct tttccgcttt ggggagaaag gggtcgcgct
      961 gctggttttt tcgtcggtgg gctatgaggt ccttctgtgg tcgctgggag ttgtgctgtt
     1021 caccaccgac ctgagcggct ggcagaggat aaaatcgctg ctgagtccgc cgtttgtgac
     1081 gctgatcacc gccctgctgt ttgtggtgct ccgtgacttg ttcacctggc ccgcgccgcc
     1141 cgggtggctg gcactggcat ggcagaccgt gcgcagcggc atgcatgcgg tggggcaggc
     1201 caccattgcc ttttcgatgc tggtcgccgg aagccgtttt gcggtgatga agcatgaagt
     1261 catgctcggc tggcgggtat ggctggtctc tttcatccgg ctcgtggctg ttccgctgat
     1321 catgattccg gtgctgggtc tcattccgat ggacgagacc gcgcgcgggc tgctgaccat
     1381 cgttgcggtg atgccggcgg cgatggccag tgtgctcttc agtgagcgtt ttggaggcga
     1441 taccgacttc attgccggcg ggctgctgct gacgcacctg tgggcgctgg tgaccgtgcc
     1501 gctttttctg gcctgggcgc tgtagaaaaa ggttcgggcg accttgacac aaatgctgtg
     1561 gaggacgtaa tcttaaagaa aggtaaagcg gtttaccccg attgaatttt gaaaggagct
     1621 tcccgtgagg ctgaaatccc ttttggttcc tgtactcgcc gccggattgc tgacctgcgc
     1681 cgcccgcgcg gaggtcattc tccagttttt taacaatacc tggaacgaaa tcgccgacga
     1741 gatcccggaa atcgccgagg cgggctacac cgcgctctgg ctcccgccgc cccagaaggc
     1801 cagcggcggt ctctccgtcg gatatgatct ctgggaccct tttgacctcg ggagtgtgga
     1861 tcagcgcagc acggtcaaga cccgctacgg cacggaggag gacctgcttc gcctgatcga
     1921 aacggctcac cgttttggcc tccgggtcta tttcgacaac atcatgaacc atcgcgcatt
     1981 cgacatcccc ggctacaacg aaaacacccc cgtggacata tatcccggca tgctccccga
     2041 ggatttccac ctgcgcgtga ccgaggaggg cttctaccgc aagtgggaca acaccgtgaa
     2101 ctggggcagc acatgggagg tccagaaccg caacctgtct gacctcattg acatcgcgca
     2161 cgaaacgccc aacgcaaact tcggcccgaa cgagggtgac acccatccca agatttcgtt
     2221 tgtccgccac ccggacaatc ccgaattcta cgactaccac ccgacgctcg gctgggtggg
     2281 attcggcacc accaacatca ccaccaacac gctggcggaa cacgaagact attacgagga
     2341 ggacgtcggc ggctacctga tgcgctccat ccgctggctg gtggataaaa caaaggtgga
     2401 cggccttcgg ctggatgccg tgaagcatgt gccgggatat ttcttcggag aacagtggga
     2461 tgccggaaag gattccagcg attccggcta ctgcggccag gcccagtggc agttcaacat
     2521 gagccgtgga ttcagtgact gggacaacca ccgcgacacc gtgttcgaca ccgagaaatc
     2581 ctatggccga aacgac
//
LOCUS       DAOQWR010000218         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_43887_length_2589_cov_2.094712, whole genome shotgun sequence.
ACCESSION   DAOQWR010000218 DAOQWR010000000
VERSION     DAOQWR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43887_length_2589_cov_2.094712"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKK36_11710"
     CDS             <1..354
                     /locus_tag="PKK36_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR95261.1"
                     /translation="CSFNLPSMLAGKMHALLFRKWANRVKGRDWYDWVWYAARKVPVN
                     LNHLSARMRQTGQWTSEVPILESELKQLLHDRVQELDVNAARRDVEPFLRNPDSVAVW
                     STPFFEDLASRLRTE"
     gene            546..>2589
                     /locus_tag="PKK36_11715"
     CDS             546..>2589
                     /locus_tag="PKK36_11715"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNR95262.1"
                     /translation="MKALQWLDRNKWFIVACVFLGINAFGIFYRGSPGSFGGQKLFAE
                     FKPGADAEFLGRDCLTWIFTEPMVSEGRIGDEAEGEPVVITPAVEGSLRWIDRDCLQF
                     KPSANWPPASTFTAKLADNIKSVAGSRLMSPRSCSFHTEDLCLKSISQADWTAEGEVL
                     LKIQFNQKVSPKVLEKYLQVQDTGNKDLQWTLPGDVVSDRILLKVSCGSRKELKIRIR
                     QGFYGEDGPLPFEKDMEYAVAIEAGLALTSLSVGSPSYENPSITAHFSRAVDLESLRR
                     SITIEPSVRFTVEEQKNWWYGKRYVISGDFAPGRNCTISFEKGLKSQDGRARLEQDIV
                     KPIFITDRTPDIKIRLQGHYLSPSGNLLLPLTSVNLDEFEVSTARVFPNNLVQYAMRS
                     GGHYSRFYGTAEDDISAVITSRTYKVKSVKNEIRDHEISLKDVLPEDPHGAYVVTLRR
                     NGAVQARRLMVVTDTGIAVKQSAGQLLVWANSIHTLEAVTNGHVQVWSEANQLLCEGY
                     TDGQGLAVLSLSREGALPFLVSIQQGQDLSFLNLTDTRLQPISAEGSLPYLSEGYEAF
                     VYSDRGIYRPGETAHIRAVVRGSRLACPAEFPVELQIRRPDGRLHSTLHSMLSSSGTA
                     EFEAPWADYDATGSYRMELKVPGSDRIIGETVIAVEDFVPPRMAVDAALDQSVLSAG"
BASE COUNT          528 a          688 c          792 g          581 t
ORIGIN      
        1 tgcagtttca atctgccgtc catgctggcg ggcaagatgc atgcactgct tttcaggaaa
       61 tgggcaaacc gggtgaaggg tcgtgactgg tatgactggg tgtggtatgc ggcccggaag
      121 gttccggtga acctgaatca tctctcggcg cgaatgcgcc agaccgggca atggacttcg
      181 gaagtgccga ttctggaatc tgaacttaag cagctgctcc atgaccgtgt tcaggaactg
      241 gatgtgaacg ccgcccggcg ggatgtggag ccgttcctcc ggaatccaga cagtgtggcg
      301 gtttggtcca caccgttctt tgaagatctg gcatcaaggc tcagaacaga ataatttacg
      361 catccgtgga tatttccctt tctggattgt gataggcccg ggcggcttca tcaggggttc
      421 tgtcgccgca gaccccgccg aattgcgtcc tgctgaaact gtcacaattg ttcacagtcc
      481 tgtcataatt ctgacacaat cttttggtct cctttgtgca tgaaaactga acaaaggagc
      541 ttttcatgaa ggcattgcag tggctggaca ggaacaagtg gtttatcgtc gcgtgtgtat
      601 ttctggggat caacgcattt ggaatatttt accggggcag tccgggaagc ttcgggggcc
      661 agaagctctt tgcagagttc aagcccgggg cagatgcaga atttcttggc cgtgactgcc
      721 tgacgtggat tttcacggag cctatggttt cggaaggccg catcggggat gaggcggagg
      781 gtgagcccgt ggtgatcact ccggcggtcg aagggtctct gcgctggatt gaccgcgact
      841 gtcttcaatt caagccctcg gcaaactggc ctcccgccag cacattcacg gccaagctgg
      901 cagacaacat aaagagtgtt gccgggagcc ggctgatgtc tccccgctcc tgcagtttcc
      961 acaccgagga tttgtgtctg aaatcaatca gtcaggcgga ctggacggcg gaaggggaag
     1021 ttctcctgaa aatccagttc aaccagaagg tctccccgaa ggttctggag aaatatctcc
     1081 aggtgcagga tacggggaac aaggatctgc aatggactct tccgggggat gtggtgtccg
     1141 accggattct gctgaaggtc tcctgcggct cgcggaagga actcaaaatc cgcatccggc
     1201 aggggttcta cggagaagac ggtccattgc cttttgagaa agatatggag tatgccgtcg
     1261 ccattgaggc gggccttgcg ctgaccagtt tgtcagtggg gtcgccttcc tatgagaacc
     1321 cctcgatcac ggctcatttt tcgcgggcag tggatctgga gagtcttcgc cgctccatca
     1381 ccatcgaacc gtctgtgcgt ttcaccgtcg aggaacagaa aaactggtgg tacgggaaga
     1441 gatatgtgat ctccggagac ttcgcaccgg ggcggaactg caccatctct ttcgagaagg
     1501 gtctcaaatc tcaggacggg cgcgcccggc tggagcagga tattgttaag ccgatattca
     1561 tcacggaccg cactccggat atcaaaatcc gcctgcaggg gcattatctg tcgccatcag
     1621 ggaatctatt gcttccgctc accagtgtga acctggatga gttcgaagtg agcaccgccc
     1681 gcgttttccc gaacaatctg gttcagtatg ccatgcgctc aggcggtcac tattcccgtt
     1741 tctacggcac ggcggaagac gatatcagcg cggtgatcac ttctaggaca tacaaggtga
     1801 agtccgttaa gaacgagatc agggatcatg aaatctctct caaggatgtg ctgcccgaag
     1861 atccgcacgg ggcgtatgtg gttaccctgc gccgcaatgg ggccgtgcag gcccgcaggc
     1921 ttatggtggt cacggacacc ggcattgcag tgaagcagtc cgccggccag ctccttgtgt
     1981 gggccaactc catccacaca ctggaggccg tcaccaacgg gcatgtgcag gtctggtcgg
     2041 aagccaatca gctgctgtgc gagggatata cggacgggca gggtctggcg gtactgtcct
     2101 tgtccaggga gggggccctg ccttttctgg tgtccattca gcaggggcag gacctgtcgt
     2161 tcctgaatct cacagacacg agactgcagc cgatttccgc cgaaggaagc ctcccgtatc
     2221 tctccgaagg ctatgaggcc tttgtgtaca gcgacagagg catataccgt ccgggagaga
     2281 ccgcccatat tcgcgccgtg gtgcgcggca gtcgtctggc gtgtccggcg gagttccctg
     2341 tggagcttca gattcggcgg cccgatggac gcctgcacag cacactgcat tcgatgcttt
     2401 cctcctccgg aacagcggag ttcgaggctc cgtgggcgga ttatgatgcc accggctcat
     2461 atcgcatgga gttgaaggtt cccggatcgg accggatcat tggggagacg gtcatcgcgg
     2521 tcgaggattt cgtcccgccg cgcatggccg ttgatgcggc gctggatcag tcggttcttt
     2581 ccgccggcg
//
LOCUS       DAOQWR010000219         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_44155_length_2579_cov_5.635895, whole genome shotgun sequence.
ACCESSION   DAOQWR010000219 DAOQWR010000000
VERSION     DAOQWR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44155_length_2579_cov_5.635895"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKK36_11720"
     CDS             <1..213
                     /locus_tag="PKK36_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNR95263.1"
                     /translation="SREKVQADVAKFSLTYPVGLAGPDVQAAYGGIRAVPTKFLLGKD
                     GSIIETFIGLVPEEELRSKIEGALAE"
     gene            234..1763
                     /locus_tag="PKK36_11725"
     CDS             234..1763
                     /locus_tag="PKK36_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNR95264.1"
                     /translation="MRRPLFIISLSALLAMHARASSPFQADARLEGDVLRVQLAAPDG
                     HWFYADQFSVEDAAGTRLEPRAGPSAIRHHDPFLNEIKDVYMGTAEFFYDGAGLQSVT
                     VKFQGCNNEVCFFPEARPFNFADGFSTERASASSADESAWRNEIKGFQVRAGDSGYMK
                     TDAFLKFLESAPRERFSLEDRGMIWILALILLGGLALNLTPCVLPMIPVNLAVIGAGA
                     RAGSRRNGFLLGAAYGTAIALTYGMLGAFVVLTGSTFGSLNAFPWFNLGIALVFVLLA
                     LGMFDVIHIDFSRFQGGPVQNNRNPFWLALTMGAVSALLAGACVAPVVISVLVLSAKL
                     YASGVRSGILLPFLLGAGMALPWPFAGMGLSFLPKPGKWMVWLRNGFGALILLLALYY
                     AHLAWTQFGITGLDEAAGGHGEIVPASQANAELADGLIRAAAEGRPVLIDFYASWCKN
                     CLAMDKTTLKDPAVQAALADYVFIKYAAENPSDPLTREVLEYFGVLGLPTYVILSPAV
                     P"
     gene            1789..2274
                     /gene="nuoE"
                     /locus_tag="PKK36_11730"
     CDS             1789..2274
                     /gene="nuoE"
                     /locus_tag="PKK36_11730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNR95265.1"
                     /translation="MNKEISTSVSAVKREDVMKIINAFRDQPGALIPLLQAVQREYGY
                     VPKEAVDLIADELGMFPVDIYGVLTFYAQFYLEPRGRHTIRVCEGTACYIMGGKDILD
                     RLKTDLGVEESKTTADGRFYLETVACLGCCGMAPAMMIDEDFYGNCTVGQLDEILKKY
                     E"
     gene            2267..>2579
                     /locus_tag="PKK36_11735"
     CDS             2267..>2579
                     /locus_tag="PKK36_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNR95266.1"
                     /translation="MSEKRKILICMGTSGIAAGATGVARLFEQELGKRNLADGFQIIR
                     TGDRGLFRDVLVDVIEPDGQRTTYEYVKAEDVVPIVEEHLIEGNPYAKRLAGKSYHDF
                     FK"
BASE COUNT          496 a          705 c          782 g          596 t
ORIGIN      
        1 agccgggaaa aagttcaggc ggatgtcgcg aaattcagcc tgacctatcc ggttggactg
       61 gccggtcccg atgtccaggc cgcctacggc ggcattcgcg ccgtgcccac taaatttctg
      121 cttggtaaag acggcagcat catcgagacc ttcatcgggc ttgttcccga agaagagctg
      181 cgttccaaaa ttgaaggggc gctggcggag taaatcacgg gagagtccca ttcatgcgcc
      241 ggccgctttt catcataagc ctttcagctc tccttgcgat gcatgcgcgg gccagttctc
      301 catttcaggc ggatgcccgt ctggagggag acgtcctgcg ggttcagctg gcggccccgg
      361 atggacactg gttctatgct gatcagtttt ctgtcgagga tgccgctgga acccggttgg
      421 aaccccgcgc cgggccctcc gccattcggc accatgatcc ttttctgaac gagataaaag
      481 atgtctatat ggggaccgca gagttttttt atgacggtgc cggcctgcaa tccgtcacgg
      541 tgaaatttca ggggtgcaac aacgaggtct gtttctttcc cgaggcgcgc ccgttcaatt
      601 tcgctgacgg gttttcaact gaacgagcat ccgcttcctc cgccgacgaa tccgcatggc
      661 ggaacgaaat caaaggattt caggtgcgcg ccggcgattc cggatacatg aagaccgatg
      721 cgttcctgaa gtttctggaa tccgctccgc gagaacgatt ctcgctggag gatcgtggca
      781 tgatatggat tcttgcgctg attctgctgg gcggcctcgc cctcaatctg actccctgtg
      841 tgctccccat gatcccggtg aatctggcgg tcattggcgc gggcgcccgc gccggaagtc
      901 gaagaaacgg attcctgctg ggcgcagcct atggcacagc cattgccctg acctatggaa
      961 tgctgggcgc attcgtggtg ctgacgggct caacattcgg ttcactgaat gccttcccgt
     1021 ggttcaatct tgggatcgcg cttgttttcg tgctgctggc tctgggaatg ttcgatgtta
     1081 ttcacataga cttttcacgc tttcagggcg gccccgtgca gaacaacagg aacccgtttt
     1141 ggctggcact gaccatgggt gcggtgtcgg cgctgctggc cggagcctgc gtggcgcccg
     1201 tggtcatatc ggttctggtc ctttcggcca aactgtatgc gtcgggtgtc cgcagcggca
     1261 tcctgcttcc gtttctgctg ggggcgggaa tggcgctgcc gtggccgttt gccggcatgg
     1321 gactctcttt cctcccgaaa cccggcaaat ggatggtctg gctccgcaac ggctttggag
     1381 cgctgattct gctgctggcg ctgtattacg cgcacctggc gtggacgcag ttcgggatta
     1441 cggggctcga tgaggcagcc ggcggtcacg gggaaattgt tccggcttca caggccaacg
     1501 cggaactggc agacggactc atccgcgccg ccgcggaggg gaggccggtc ctgattgatt
     1561 tctatgcaag ctggtgcaag aactgtcttg cgatggacaa aaccaccctc aaagaccctg
     1621 cggtgcaagc ggccctcgcg gactatgttt tcataaaata tgccgccgag aatccgtccg
     1681 atccgctgac cagggaagtt ctggaatatt ttggtgtgct tggtttgccc acttatgtca
     1741 tattgtcgcc cgccgttccc tgatggagcg tgcgcatgaa actttgcgat gaataaagag
     1801 atttcaacat ctgtatccgc ggtgaagcgc gaagacgtga tgaaaatcat caatgccttc
     1861 cgcgatcagc ccggcgcgct gattcccctg ctgcaggcgg tgcagaggga atacggttat
     1921 gtgccgaaag aggccgtgga cctcattgcc gatgaactgg gcatgttccc tgtggacatc
     1981 tacggtgtgc tgacctttta cgcccagttt tatctcgagc cgcgcggccg gcacaccatc
     2041 cgcgtctgcg agggcacggc ctgctatatc atgggcggaa aggatattct ggaccgcctg
     2101 aaaacggatc ttggcgttga ggagtccaaa accacggccg acggccggtt ctaccttgaa
     2161 accgtcgcct gccttggatg ctgcggcatg gccccggcca tgatgattga tgaagatttc
     2221 tacgggaact gcacagttgg acagcttgat gaaattctga agaagtatga gtgaaaaacg
     2281 taaaatcctg atctgcatgg gcaccagcgg cattgccgcc ggcgcgaccg gtgtcgcccg
     2341 cctttttgag caggagctgg gcaagcggaa tctggcagac ggatttcaaa ttatccggac
     2401 cggagatcgc ggcctcttcc gcgatgtgct ggtggacgtt atcgagcccg atggccagcg
     2461 gacgacctat gaatatgtga aggcggagga tgtcgttccg attgtggaag agcatctgat
     2521 cgagggtaac ccttatgcga agcgcctcgc gggcaagagt tatcatgatt tcttcaaga
//
LOCUS       DAOQWR010000220         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_44856_length_2553_cov_3.742594, whole genome shotgun sequence.
ACCESSION   DAOQWR010000220 DAOQWR010000000
VERSION     DAOQWR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44856_length_2553_cov_3.742594"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKK36_11740"
     CDS             <1..321
                     /locus_tag="PKK36_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95267.1"
                     /translation="VLPSDYAVFVHFRAGTQRFQDDHILLEHIPDAVIREQSRRETFR
                     AQRVFRLPYDITAGSVELEAGLYNRATGKRLRQGRSLINNPRSIRLDGVLRVLQPDSG
                     RKGL"
     gene            318..974
                     /locus_tag="PKK36_11745"
     CDS             318..974
                     /locus_tag="PKK36_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNR95268.1"
                     /translation="MKFISRFPGILRICLIGGTAFVLSACEQTGDGGLGSGHNFGDND
                     PNIVYCLGDSITQGAQTPGVAPYPAVLSGMIGKTCINGGIGGTTSASGVSRVNGVISS
                     VKPGYMIIFYGHNDIYAGYSHEQTRENIRYMINACRAAQVIPIVCNLNPVYFGHGVFA
                     GEVRARSDSISSVANEEGVKLANIMSAFGNDESLLSADGLHPSQEGQNVIASTLANIF
                     "
     gene            complement(1070..1306)
                     /locus_tag="PKK36_11750"
     CDS             complement(1070..1306)
                     /locus_tag="PKK36_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNR95269.1"
                     /translation="MMNIKINHTAHLKLKGIEPGQTASLEPGTSVADLLRRAGVQESH
                     LQFVTPYVNGKEVRIHHVLQDNDELKLFLPVGGG"
     gene            1402..2502
                     /locus_tag="PKK36_11755"
     CDS             1402..2502
                     /locus_tag="PKK36_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR95270.1"
                     /translation="MTAILMTAGLLASGCADRSPPRPVFPGEHWEHIAPDRAGFSPDG
                     LAEFVKKVGGHGVVVRYGRVVDYWGHYAHLLDVASAVKPIYAHLVYMADEDGLIGSLD
                     EKVSVHAPNLERINEELNFKDRDITWRHLLQQTACYGVEEEPGTAFNYSDYQLALLAD
                     TLVFKVHKLTYSEKTAQSLDDYLTGPLRMQDSATLKHPNSLPGRLRISVRDFARFGLL
                     YLNKGRWGRRQIVPRKLAEQAISTPLPANFPRTEQVEAEMIGNQRSLGAGMNLEPHLG
                     SYSYLWWVNGTDKNGQRLLPALPEDTFLAQGHSGHDVLLVIPSLDLVVCWVDAFPKKT
                     HATRFSEGRKNVNKAAELLMAALTPGFEEARP"
BASE COUNT          545 a          699 c          745 g          564 t
ORIGIN      
        1 gtcctgccct ccgactatgc ggtgtttgtg catttccggg cggggacaca gcgattccag
       61 gacgatcata tcctgctgga acatatcccg gatgcggtaa ttcgcgagca gtcccggaga
      121 gaaacattcc gcgcgcagcg ggtgttccgg ctgccctacg acatcacggc cggctccgtg
      181 gagcttgaag cgggcctcta taaccgggct acaggaaaaa gattgcgtca ggggcggtct
      241 ttgatcaata atccgcgctc tataaggttg gatggagtat taagggttct ccagccggac
      301 agtggaagga aaggtctatg aaattcatca gtcgttttcc gggcattttg aggatatgtc
      361 tgataggcgg caccgcgttt gtgctctccg cctgtgaaca gaccggcgat ggcggtctcg
      421 gcagcggaca taactttggc gacaatgatc ccaatatcgt ctactgtctc ggggacagta
      481 ttacccaggg agcccagacc cccggcgtgg ctccataccc ggccgttctc tccggcatga
      541 tcgggaaaac ctgcatcaac ggaggcatcg gcggcaccac ctctgcaagc ggcgtcagcc
      601 gcgtgaacgg ggtcatttcc agcgtcaaac ccggctatat gatcatcttt tacggtcata
      661 acgacatcta tgccggatat tcgcatgagc agacgcgcga aaacatccgt tatatgatca
      721 acgcctgccg cgccgcccag gtgattccca ttgtctgcaa tctgaacccc gtttatttcg
      781 gccacggcgt gtttgccggc gaagtgcgcg cgcgttcgga cagcatttcc agcgtggcca
      841 atgaagaagg cgtgaaactt gccaatatca tgtcggcctt cgggaacgac gagtcgctgc
      901 tttcggccga cggactgcat cccagccagg aaggccagaa cgtcatcgcc tccacgctgg
      961 ccaacatttt ctgaccggcg cctgaaaagt tccaatgact ggaagttctg gctgaaaaac
     1021 ttccaatggc tggaactttt tattccgaaa cttccagtga ttggaacttt cagccgccgc
     1081 cgaccgggag gaacagtttg agttcgtcat tgtcctgcag cacatgatgg atgcggactt
     1141 ccttcccgtt gacatagggg gtgacaaact gcagatgcga ttcctgaacg ccggccctgc
     1201 gcagcagatc ggccacggat gttcccggct ccaggctcgc cgtctgcccc ggctcaatgc
     1261 ctttgagttt caagtgggcc gtgtggttta tctttatatt catcatgttt aatatacatg
     1321 aaaccgccct tacttcaatc ccgccggttt aatccttgtc ccgtgcgtcc gccgcagttt
     1381 agaatgaaaa aaatgaaaat tatgacggct attttgatga cggcggggtt gctggcatcg
     1441 ggatgtgcag acaggagtcc gccgcgtccg gtgttcccgg gagaacactg ggagcacatc
     1501 gctccggacc gcgccggttt ttcgcctgac ggcctggcag aatttgtgaa gaaagtcggc
     1561 ggacacggtg tggtggtgcg ttacggccgc gtggtggact actgggggca ttatgctcat
     1621 ctgctggatg tggcgtcagc cgtaaagccc atctatgccc atctggttta tatggccgat
     1681 gaggatggcc tgatcgggag cctggacgaa aaggtcagcg tgcatgcgcc gaatcttgag
     1741 cggatcaatg aagaactgaa cttcaaggac agagacatca cctggcggca tttgctgcag
     1801 cagactgcgt gctatggcgt cgaggaggaa ccgggcacag cattcaacta cagtgattat
     1861 caactggccc tgctggccga tacgcttgtg tttaaggtgc acaagctcac gtacagtgag
     1921 aaaacagccc aatcgcttga tgactatctg acgggtccgc tgcgcatgca ggattccgcc
     1981 acgctcaagc acccgaactc tcttcccggc cggctcagga tttccgtccg tgacttcgcc
     2041 cgtttcggcc tgctgtattt gaataaaggc aggtggggcc ggcggcagat tgttccccgg
     2101 aaactggcgg aacaggcgat cagtactccg ctcccagcca attttccacg cacggaacag
     2161 gtggaagcgg aaatgatcgg caatcagaga agtcttggcg cgggaatgaa tctggagccg
     2221 catctgggca gctacagtta tctctggtgg gtcaatggaa cggataagaa cggtcagcgc
     2281 ctgctcccgg cgcttccgga agataccttc ctcgcgcagg ggcattccgg ccacgatgtg
     2341 ctgctggtca tcccctcact ggatctggtg gtatgctggg ttgacgcgtt tccgaaaaag
     2401 acccatgcaa cacggttttc tgaaggccgg aaaaatgtca acaaggctgc cgagcttctg
     2461 atggcggcgc tgacgcccgg ttttgaggag gcccgtccgt gaaggtgcag cccggcagac
     2521 tgcggcagca gatcatgctt tccgaggaga gca
//
LOCUS       DAOQWR010000221         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_46464_length_2496_cov_5.551413, whole genome shotgun sequence.
ACCESSION   DAOQWR010000221 DAOQWR010000000
VERSION     DAOQWR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46464_length_2496_cov_5.551413"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>2496
                     /locus_tag="PKK36_11760"
     CDS             176..>2496
                     /locus_tag="PKK36_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95271.1"
                     /translation="MGRKLFAFCSYALAAGITVSTAHAANHFVETFDTLSCAYTDSST
                     VLPSGTWTLNNVFSETATESRGGTGHAARINDDIAGAHLTTPVLNSVGTVSFYYRELN
                     SGGGTFKVQKSVSGGTFTDVGSQAFSGTTYTFYTIDVNDSSSSIAIRILNDNQKGHLI
                     IDDMTVTDYGGAPATNPPVLNEIGSKSVTVSNTLQFTVTATPTESDTVTLSASNLPSG
                     AYFYPTNESGSFIWASATPTGSYNVTFYAADDDGVDSETITITVNPGSGGGGEGGLDI
                     SGWSVVQSNSTSTYTFPSGTTAAPNSYVIIGRYTDKASFETAWGVTLSSDVVYLNGNN
                     LMPMINGAEVYGLYNDSSTLMDGFTPASLNPSGNSIQRTTLGGDASDSGNWSSSAMAN
                     ATPGSGASGNGTAGLRISEYSDAGNYINEFVELYYDASTTLPSNQPPVLAAIGNKTTE
                     ELTALQFTISAADPADGDAITLSMSNAPAMAVLSSTNGEGTFTWALPTPDGVYTMTFY
                     AVDKDGAASETITVTVTPPSASITSNLWDFEDGWQGWTNYSRANNSYNWSRVNFPGAE
                     STAWKMEMEGTSSDDWLISPALNLSAVTDPVLTYWTKRSNRDITIQVKVSTNYPGTGD
                     PLAAGISWVNLPYTRPSRSDVWAGHTNNLSAYVGAEQVYIAYSYSSGGDSTLWSVDQI
                     RYSATATVESNAPPIMAPIGDKSIMISNNLMFPVQSVDPVDGDIISLTVSNLPAGAEF
                     TNDNGNGYFTWVNASPDGVYTTTFHAADKDGFDA"
BASE COUNT          598 a          733 c          633 g          532 t
ORIGIN      
        1 actccgtatt tggtttgcag gcaataacct cacgtcggca acgctatatc ttccgggact
       61 tcatatccag aaaatacgca gtgaaaaaaa tacagccaga cactaaactt cttttcattt
      121 atgaggtata ttcagtccag ttatacaaga aacaagccga acaaggatga gcagtatggg
      181 gagaaagcta tttgcatttt gttcatatgc gctggcggcc ggcatcacgg tttctaccgc
      241 gcacgcagcc aatcattttg ttgaaacatt tgacaccctg tcctgtgcgt atacagactc
      301 cagtacagtg ctgcccagcg ggacatggac gctgaacaat gttttttctg aaacagcgac
      361 cgaatcccgc ggaggaaccg gtcatgccgc gcgcatcaac gacgacatag ccggcgcgca
      421 cctgaccacg cccgtactca attccgttgg aactgtttcc ttctattatc gtgaactcaa
      481 ttccggcgga ggaacattta aagtccaaaa atcggtttcg ggcggcacct tcaccgatgt
      541 cggatcgcag gctttctcgg gcacaacgta cactttttac actatcgatg tgaatgattc
      601 cagcagttcc attgcgattc gcatcctcaa cgacaatcag aagggccatt tgatcataga
      661 tgatatgacc gtgactgatt acggcggggc ccccgccacc aacccgccgg tcctgaatga
      721 gattgggagc aaatccgtaa cggtcagcaa tacgcttcag ttcaccgtga cggccacgcc
      781 gacggaaagt gacacagtta cgctctcagc cagcaatctg ccttccggag cgtatttcta
      841 tcccaccaat gaatccgggt ccttcatctg ggccagtgcc accccaaccg gcagttacaa
      901 cgtcacattc tacgccgcgg acgatgacgg ggttgattcc gaaacaatta ccatcacggt
      961 caacccgggc agtggaggcg ggggcgaagg cggacttgat atcagcgggt ggtcagttgt
     1021 gcagtcaaac tcaacatcga cttacacttt tccctcgggg acgacggccg caccaaacag
     1081 ctatgtgatc ataggccgct acacggataa agcatcattt gagacggcct ggggtgtcac
     1141 gctgtccagc gacgttgtat acctgaacgg gaacaacctc atgcccatga tcaatggagc
     1201 cgaagtctac ggcctctaca atgattccag cactctcatg gacggcttca cccccgcctc
     1261 gctgaatccc tccggaaatt caattcagcg caccacactc ggaggcgacg cctccgattc
     1321 agggaactgg tcttcctctg ccatggccaa tgccacgccc ggcagcggtg catccggcaa
     1381 cggcaccgcc gggctgcgta tcagtgaata ctctgacgcg gggaactata tcaatgagtt
     1441 tgtggagttg tattatgatg cctcgaccac cctcccctcc aaccagccgc cggtgctggc
     1501 cgccatcggc aacaaaacaa ccgaagaact gactgcactg cagttcacga tttcagcagc
     1561 cgatcctgcg gacggtgacg ccatcaccct ttcgatgagc aatgctcccg ccatggcggt
     1621 gctgtcctcc accaatggag aagggacctt cacctgggca ctgccgaccc cggacggggt
     1681 ctacaccatg accttttatg ccgtggacaa ggacggggcg gcgtccgaga ccatcaccgt
     1741 caccgtaacg cccccgtccg cctccattac cagcaacctc tgggatttcg aggacggctg
     1801 gcagggctgg accaattaca gccgcgcgaa caactcatac aactggtcgc gggtgaactt
     1861 tcctggagcg gaaagcacgg catggaaaat ggagatggag ggcacgagca gtgacgactg
     1921 gctgatcagt ccggcgctga acctttccgc tgtaaccgat ccggttctga cctactggac
     1981 gaagcgctca aaccgtgaca tcaccattca ggtgaaagtg tccacgaatt atccggggac
     2041 aggggatccg ctcgcagccg gcatttcctg ggttaatctt ccttataccc ggccttccag
     2101 aagcgatgtc tgggcgggac atacgaataa tttatccgcc tatgtcgggg cagaacaggt
     2161 atatattgca tattcgtaca gcagcggtgg agattccaca ctctggagcg ttgaccagat
     2221 ccgatactcc gccacagcga ctgtcgaatc caatgccccg ccgatcatgg ctccgattgg
     2281 cgacaaatcc atcatgattt caaacaatct gatgttcccg gttcagtccg ttgatcccgt
     2341 tgacggcgac atcatctccc tgaccgtcag taatctgccc gcgggagcgg agttcacgaa
     2401 cgacaatggg aacggttatt tcacatgggt gaatgcatca cctgacggcg tttacaccac
     2461 cacctttcat gcggcggaca aggacggttt tgatgc
//
LOCUS       DAOQWR010000222         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_46579_length_2492_cov_5.926139, whole genome shotgun sequence.
ACCESSION   DAOQWR010000222 DAOQWR010000000
VERSION     DAOQWR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46579_length_2492_cov_5.926139"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKK36_11765"
     CDS             <1..187
                     /locus_tag="PKK36_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNR95272.1"
                     /translation="VCTGASMKQAYYRTFVAEDAAKSFVAACAVGRPRFLSDEQVAEL
                     QSLEAGAYREGLAAEEQ"
     gene            254..1540
                     /locus_tag="PKK36_11770"
     CDS             254..1540
                     /locus_tag="PKK36_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HNR95273.1"
                     /translation="MRAAVHPGGGGRFNCIFCASFIHDQAHVEKIRSGYERALTAAGA
                     SCVTAAEDKLPTCIAQEDALVYFVLTGGVENQVLDLWRKRQQYVPGRPVFLVAHPLHN
                     SLPASLEILARFRQDGVAGEILYLNEPADKAGMGCIISAVHMLNVHRQLAADRIGMIG
                     EPSPWLIASSPDPELIRRLFGPEVVRIPMQELAPYASAAAGAAAAADEFRKIFPKTVE
                     PKDDDLLASARIYLGLKALVEKHELTALTVRCFDFVLKNKATGCLALARLNDEGIIAG
                     CEADLVSLSGMLWAFRVTGSLPWMANPSHIIPADNILSLAHCTVPSKLVTNLVLRSHF
                     ESGLGIGVQGDFEPGPVTLLRLGGADMNSIWLAQGEIIEAARSPDLCRTQIRVRLDPD
                     CPAGALLKNPLGNHMVVIPGRHVSLLREAWPLTVSV"
     gene            1612..>2492
                     /locus_tag="PKK36_11775"
     CDS             1612..>2492
                     /locus_tag="PKK36_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR95274.1"
                     /translation="MKLETQCLHAGQVPDPATLSCAVPVYRTSAYQFKSTDHAANLFG
                     LRELGNIYTRLMNPTHDVLEKRMAALDGGAAALALASGTSAIFYAVANICRAGDEIVS
                     SANLYGGTYTQFHDILPQFGIKAHLVDVRDPQNFAKAITPKTKLLFTESIGNPSLDVA
                     DISAIAKIAHDAGLPLVVDNTFATPALLRPIEHGADIVVHSLTKWLGGHGVGIGGIVV
                     DSGKFDWTSGKFPLLSEPDASYHDIRFAKDLGPLNPLAYILRMRLVPLRNLGACISPD
                     NAWMFLQGIETLPLRMQR"
BASE COUNT          451 a          751 c          757 g          533 t
ORIGIN      
        1 cgtctgcacg ggcgcaagca tgaagcaggc ctattaccgg acatttgtcg cggaggatgc
       61 cgccaagtct ttcgtggccg cctgtgcggt cggccgtccg cgcttcctgt ctgatgagca
      121 ggtggccgaa ctgcagtcgc tggaggccgg agcttatcgc gaagggctgg cggccgaaga
      181 gcagtgaccc gcgtggcgtg gccgctgttt ttccgctgga tatattccgc ggagcgcgct
      241 accatgcctt caaatgagag cagcggttca tccgggaggg ggcggtcgtt tcaactgcat
      301 tttctgtgca tcctttatcc acgaccaggc ccatgtggaa aaaatcaggt ccggctatga
      361 gcgcgctttg acggcggccg gtgcttcctg tgttacggcg gccgaagata agctccccac
      421 ctgcattgcg caggaggatg ccctcgttta tttcgtgctg acggggggcg tggaaaacca
      481 ggttttggac ctttggagaa aacgccagca gtatgttccg ggccgccctg tttttcttgt
      541 cgcgcacccg ctgcataatt ccctgccggc gtccctggag attctggccc gttttcgcca
      601 ggacggagta gcgggagaaa tcctgtatct caacgaaccc gccgacaagg cgggcatggg
      661 gtgcatcatt tccgcagtgc acatgctgaa tgtccatcgg cagcttgcgg ctgaccggat
      721 cggaatgatt ggagaaccgt ccccctggct gattgccagt tcaccggatc cggaactgat
      781 ccggcgcctc ttcgggcccg aggtcgttcg aatccccatg caggaactgg cgccttacgc
      841 ctcggccgcg gccggcgctg ccgctgccgc cgatgagttc agaaagattt tcccgaagac
      901 cgtggagccg aaagacgacg acctgctggc ttctgcccgc atttatctgg ggttgaaggc
      961 gcttgttgaa aagcatgaac tcacggcgct cacagttcgc tgttttgact ttgtcctgaa
     1021 aaacaaggcc acgggttgtc ttgcactggc gcgactgaat gatgagggca taatcgccgg
     1081 ctgcgaagcc gatcttgtca gcctgtccgg catgctctgg gcgtttcgcg tgacgggcag
     1141 cctgccgtgg atggccaatc cgtcgcacat catccctgct gataacatcc tgtcactggc
     1201 ccactgcacg gtgccttcaa aactggtcac caatctggtg cttcgctcgc attttgaatc
     1261 cggcctgggc attggagtgc agggggattt tgaaccggga cccgtcacgc tgctccggct
     1321 gggcggtgcg gacatgaaca gcatctggct ggcgcagggt gaaatcatcg aagcggcccg
     1381 cagccccgat ctctgccgca cacagatccg ggtccggctg gatccggact gccccgcggg
     1441 ggccctcctg aaaaatccgc tgggcaatca catggtcgtc atcccggggc ggcatgtcag
     1501 cctgctgcgt gaggcatggc ccctgacggt gtctgtctga accgcttttc tggctttttt
     1561 ttgcgggcct cccgttgtag catcattctt cattaagttc aggagaatgc catgaagctt
     1621 gaaacgcaat gtctgcacgc gggccaggtt cccgatcccg ccacgctttc ctgcgcggtg
     1681 ccggtgtacc ggaccagcgc ttatcagttc aaaagcacgg accacgccgc aaacctgttt
     1741 ggtctcaggg aacttggaaa tatttacacg cggctgatga accccacgca cgacgtgctg
     1801 gaaaagcgca tggctgcgct ggacggcggc gccgcggctc tggcgctggc ctccggcacc
     1861 agcgcgattt tctatgccgt tgccaatatc tgccgggcgg gcgacgaaat tgtctcctcc
     1921 gccaatctgt acggcgggac ctatacgcag tttcacgaca tcctcccgca gttcggcatc
     1981 aaggcgcatc tggtggatgt ccgcgatccg cagaatttcg caaaagccat caccccgaaa
     2041 acaaaactgc tgtttacgga gtctatcggc aatccctcgc tggacgtggc cgatatttcc
     2101 gccatcgcaa aaatcgcgca cgatgcgggt ctgcccctgg tcgtggacaa cacctttgca
     2161 actccggcgc ttctgcggcc gatcgagcac ggcgcggaca tcgttgtaca ctcgctgact
     2221 aaatggctgg gagggcatgg agtcggcatc ggcggcattg tggtggattc cggcaagttc
     2281 gactggacca gcgggaaatt cccgcttctg tcggagccgg acgcaagcta tcacgacatc
     2341 cgtttcgcga aggatctcgg cccgctgaat ccgctggcgt acatcctgcg gatgaggctg
     2401 gttccgctgc gcaacctcgg cgcgtgcatc tcgccggaca atgcgtggat gttcctgcag
     2461 ggcatcgaga cgctgcccct ccggatgcag cg
//
LOCUS       DAOQWR010000223         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_47703_length_2455_cov_4.405000, whole genome shotgun sequence.
ACCESSION   DAOQWR010000223 DAOQWR010000000
VERSION     DAOQWR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47703_length_2455_cov_4.405000"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1732
                     /locus_tag="PKK36_11780"
     CDS             197..1732
                     /locus_tag="PKK36_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNR95275.1"
                     /translation="MSSEVQVEVFGRPVYRDGILSPEILNEISDVESLAGLARMLRGQ
                     YALIRRAPECVLAVTDPLGTYPLYYSTTEVSSVARKVSASGRVSREAFFSYAALGKNA
                     TSGGMYEDAVRVPPGSVLLMGAGGAARVKPYFRLADLLGLPILRAEDAHDEFLNLAGS
                     YLQAVAGGEPSAASLFSSGIDSALIVMLLKRCCPNLPLAGWSVDYPRCFSPRYSEYTQ
                     AAINAEKLSLPLRKAFCCAADFYNALEQLLADHPDIPPCDRAMPIQYISLKRAGNERP
                     GVYFTGFNADRPYCGHGHWADMARRYSRALNPSAASLPAARIAEMLCYPQPRRVKLME
                     QVVLAAGYSLDEYYCWIRDFKERCVEHVREYGNIDFLQLIMILSEEEIGDLWAAEHLL
                     LERHYGMNFISPFGDISIVRIGFSLPLEFHYSDGGTKRFLREVLRKETGMTLNKRAFP
                     SPMRFWAMRPQNLRLPHTPAMKKMYSGMWRQNLIAGGRSYAAAHDALALGLWLEKQDH
                     ISR"
     gene            1729..>2455
                     /locus_tag="PKK36_11785"
     CDS             1729..>2455
                     /locus_tag="PKK36_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95276.1"
                     /translation="MNQSAVIITGGLIIIAMARAYLRFRDPLHPLMFLGPLCFYVYVY
                     QPLMLGKAGLLNYYFPDEGALEFVQAAVLLFILVFCLGLLKISIPSEALRSLTSHPFD
                     PSPRVKALFRRAALIMGTSGLLGFAYMSAAGPGILKAYSQYKGALVAVSGYIGESTLL
                     TIPACILYIFSRQGEKWRFRHYALLAAFAMPHFLHAFLGARRGPAFSIMVALLVPWFM
                     TRSRRPSLRTVFTALLLVGVVVLF"
BASE COUNT          433 a          684 c          718 g          620 t
ORIGIN      
        1 aggactgtct ggggctctgc ttctatatgc ggcggaatat tctcctgagg cttgcacttg
       61 tgcctgtggc tgcgattatt gctgtttttc atgtcggaca caggaaaccg gtttcacgca
      121 aaacttacag ggcgtttcgc ggactgccag gccatgcacc ccgactgatc aatactgtac
      181 tgccagcccc tttgacatga gcagcgaagt ccaggttgaa gtcttcggcc gtccggttta
      241 tcgcgacggc attctgtccc ccgagatcct gaatgaaata tctgatgtcg agagccttgc
      301 aggccttgcc cggatgcttc gcgggcagta tgcgctgatc cgccgcgccc ccgaatgtgt
      361 tctggcagtt acggacccgc ttggaaccta tccgctctat tacagcacaa ccgaagtgtc
      421 gtccgtggcc cggaaggttt ccgcgtcggg acgtgtgagc cgggaagcgt ttttcagtta
      481 tgccgccctt gggaagaatg cgacttctgg cgggatgtac gaggatgctg tgcgtgttcc
      541 gccgggttcg gttttgttga tgggggctgg cggagcagcc cgggtgaaac cgtatttccg
      601 tctggcggat ctgctgggtc ttccgattct acgggcggaa gatgcgcatg acgaattcct
      661 taatctggcg ggaagctacc ttcaggctgt ggctggcgga gaaccttccg ccgcgtcttt
      721 gttctcctcc ggaatagatt ccgcactgat tgtcatgctg ctgaagcgct gctgcccgaa
      781 ccttcctttg gcgggatggt cggttgacta tccccggtgt ttcagccccc ggtattcaga
      841 gtatacgcag gcggccatca acgcggagaa gttatccctg cccctgagaa aagccttctg
      901 ctgcgccgcg gatttttata atgccctgga gcaactgctg gctgatcacc cggatatccc
      961 tccctgcgac cgggcaatgc cgattcagta tatttccctg aagcgcgcgg ggaatgaacg
     1021 tcccggggta tatttcaccg gattcaatgc ggatcgtccg tactgcggac acggacattg
     1081 ggcggacatg gcgcgcagat attcgcgcgc cttgaatcct tccgcggcat cgctccctgc
     1141 tgccaggata gcggagatgc tttgttatcc gcagccccgc cgtgtaaagc tgatggagca
     1201 ggtggtcctt gctgcgggat attcattaga cgagtactac tgctggattc gggatttcaa
     1261 ggagcgatgt gtggagcatg tgagggaata cgggaatatt gatttccttc agttgatcat
     1321 gatcctttcc gaggaggaaa tcggcgatct ctgggcggcg gaacatctgc tgcttgaacg
     1381 gcattatggc atgaatttca tcagcccttt cggagatata tccatcgtgc ggattggttt
     1441 ttcgcttccg ctggagtttc attattcaga cggcggcacc aaacgtttcc tgcgtgaggt
     1501 gctgcgcaag gaaaccggca tgacgctgaa caaacgcgca ttcccttctc ccatgcggtt
     1561 ctgggccatg cgtcctcaga atctgcgcct gccgcatact ccagcgatga agaagatgta
     1621 ttcgggcatg tggcggcaga atctgatagc cggcggaagg tcgtacgcag cggcgcatga
     1681 tgcccttgcg cttggcttgt ggcttgaaaa acaggaccat atctcccgat gaatcagtct
     1741 gcagtcatca tcaccggcgg cctcattata attgcgatgg cccgggctta tctgcgtttc
     1801 agggatcctt tgcatccgct gatgttcctc gggccactct gcttctatgt ctatgtctat
     1861 cagccgctga tgctcgggaa agccggactg ctgaactact acttcccgga tgaaggcgct
     1921 ctggaatttg ttcaggcggc agtactgctg tttatcctgg tgttctgtct ggggcttctg
     1981 aagatcagca tcccttccga agcgcttcgc agccttacat cccatccctt tgatccgtca
     2041 ccgcgcgtga aggccctctt ccggcgggcc gcgctgatca tgggaacctc cggcctgctg
     2101 gggtttgcct acatgtcggc tgccggcccg ggcattctga aagcctacag ccagtacaaa
     2161 ggggcgctgg tggcggtgag cggctatatc ggcgagagca cgctcctgac catccccgca
     2221 tgcattctgt atattttctc gcggcagggt gaaaaatggc gcttccgtca ctatgcgctg
     2281 ctggcggctt tcgccatgcc tcattttctc catgcgtttc ttggagcgcg ccgcggaccc
     2341 gctttcagca tcatggtggc gctgctggtc ccctggttta tgacccgttc gcgccggcca
     2401 tcgctgagaa cggtgttcac cgcactgctg ctggtcggcg tggtggtgtt gttcc
//
LOCUS       DAOQWR010000224         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_47756_length_2453_cov_5.859049, whole genome shotgun sequence.
ACCESSION   DAOQWR010000224 DAOQWR010000000
VERSION     DAOQWR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47756_length_2453_cov_5.859049"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..384
                     /locus_tag="PKK36_11790"
     CDS             160..384
                     /locus_tag="PKK36_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95277.1"
                     /translation="MKAVSSRCTPKRRKGGATFIILRQELIWISKTSTIIDEEPKNAE
                     IWPKMAKNRLFMMNFGVLGPFLGFFGQFSG"
     gene            complement(392..1333)
                     /locus_tag="PKK36_11795"
     CDS             complement(392..1333)
                     /locus_tag="PKK36_11795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR95278.1"
                     /translation="MNAEQKKVVMVVPFLNEQENIPVLYDRVCKAMEGQPEQFEILFV
                     NDGSSDGSPDWIRNKAKEDPRVRMITFSRNFGHQLAITAGMDYADGDAVVIMDADLQD
                     PPEVIPQLLEKWREGNEVVYAVRAARVGETWLKKFLAATFYKIFHAMAKVNVPLNTGD
                     FRLVDRKVVESLREVRELHRFVRGLTCWVGFRQCAVHYERAARLAGETKYPVWKSMNL
                     AWDAITSFSGTPLRWVTGIGLLFSLCGLIFMLHIVIQTMLHPETVAQGWASLAALTLI
                     MGGVQLICIGLLGQYVSRIFEETKKRPLYLIREKIGR"
     gene            complement(1330..1944)
                     /locus_tag="PKK36_11800"
     CDS             complement(1330..1944)
                     /locus_tag="PKK36_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR95279.1"
                     /translation="MSQLQKKAVFIDRDGVLNEMVYDEDHGIMDSPRRPEQVVMMAGA
                     GEFVRAVRELGFLAVVATNQPGIAKNTLTPENLAAVNERLAELMRAEGGQWDALKFCP
                     HHPDGGYNPRREYVMKCECRKPRPGMLLEAAMEMNIALDRSWMIGDGLTDIQAGHAAG
                     CRTILMTQLKIGQVERFFHTDGREPDYICRDLAGALSIIKKESP"
     gene            complement(1941..>2453)
                     /locus_tag="PKK36_11805"
     CDS             complement(1941..>2453)
                     /locus_tag="PKK36_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HNR95280.1"
                     /translation="SRWGLRVKYSFEDPRALMGTGGALLNALDLLEDTFFVLYGDSYL
                     PMDYRAMAAAFEQAGTPAIMSVYRNRGLFDKSNVRIANGRVVFYSKSARPDEVDCIDY
                     GFTGYRKELLAEYRDAPLPLDLESILRRQVELDRLAAYEVTERFYEIGKPEGLAELEE
                     YLKTRHTAGS"
BASE COUNT          505 a          740 c          684 g          524 t
ORIGIN      
        1 gatcacggcg gtgatgtagt agcgcctctg tgaggcgcat tctgaaatat ataccgctcg
       61 cagagcggtt actacaccag aaccccctgc aaataaaggc gttcccggct gtcttaatca
      121 tggaacgacg aatatattaa aagccgtctg tgaattcgcg tgaaagcggt gtccagtcgc
      181 tgcactccaa agcgccgcaa aggcggcgca acgtttatca ttttacgcca ggagttgatt
      241 tggatatcaa agacttccac gataatcgac gaagaaccga aaaacgccga aatatggccg
      301 aaaatggcca aaaatcgcct gtttatgatg aatttcggcg ttttggggcc gtttttgggc
      361 tttttcggcc aattttcggg ctgaaacagc ttcagcggcc gattttttcg cggatcagat
      421 agagcggacg cttttttgtt tcttcaaata tccggctgac atactgcccc agcaggccga
      481 tgcagatgag ctgcacgccg cccatgatca gtgtcagcgc cgccagcgaa gcccagccct
      541 gcgccacggt ttccggatgg agcatggtct ggattacgat atgcagcatg aaaatcagtc
      601 cgcaaagcga aaacagcagg ccgatgcccg tcacccaccg gagcggtgtg ccggaaaacg
      661 atgtgatggc gtcccacgca aggttcatgg atttccagac cggatatttc gtttcgcccg
      721 ccagccgcgc ggcgcgctcg taatgcaccg cgcactgcct gaaaccgacc cagcaggtca
      781 gcccgcgcac gaagcggtgc agttcacgca cttcgcgcag ggattcgacc accttgcggt
      841 ccaccagccg gaagtcgccg gtgttgagcg ggacattcac tttagccatc gcgtgaaaga
      901 ttttgtagaa cgttgcggcg agaaattttt tcagccatgt ttcgcccacc cgcgcggcgc
      961 gcacggcata gacgacttca ttgccctcgc gccatttttc cagaagctgc ggaatcactt
     1021 ccggcggatc ctgcaggtcg gcatccatga tgaccacggc atcgccgtcg gcatagtcca
     1081 tgcccgccgt gatcgcgagc tgatggccaa agttgcgcga gaaagtgatc atccgcacgc
     1141 gcgggtcttc cttcgccttg ttccggatcc agtcaggcga gccgtcggaa ctgccgtcgt
     1201 tgacgaacag gatctcgaac tgctccggct ggccttccat ggccttgcag acgcggtcat
     1261 agagcacggg gatgttttcc tgctcgttga gaaacggaac caccatcacg actttcttct
     1321 gttcagcgtt catggacttt cctttttgat gatgcttaaa gccccggcaa gatcccggca
     1381 gatataatcg ggctcgcgac cgtcggtgtg gaaaaaccgc tccacctgcc cgattttcaa
     1441 ctgagtcatg agaatggtgc ggcatccggc ggcatgaccc gcctgaatgt cggtgagccc
     1501 gtcgccgatc atccagcttc ggtccagcgc gatgttcatt tccatcgcgg cttccagcag
     1561 catgccgggc cggggcttgc ggcattcgca tttcatcaca tattcccgcc gcgggttgta
     1621 ccctccgtcc gggtgatgcg ggcagaactt cagggcatcc cactgcccgc cttcggccct
     1681 catcagttca gccaggcgct cattgacggc cgcaaggttt tcaggagtca gcgtgttctt
     1741 ggcaatgccc ggctgattcg tggccaccac agccagaaag cccagctcgc gcaccgcgcg
     1801 gacaaactcc cctgccccgg ccatcatgac gacctgctcc ggccggcgcg gggaatccat
     1861 gatgccgtgg tcttcgtcgt agaccatttc attgagcacc ccgtcgcggt caatgaacac
     1921 ggctttcttc tgcagctgac tcatgatccg gcagtatggc gggttttcag atattcttca
     1981 agctccgcaa gaccttcggg cttgccgatt tcatagaagc gttccgtgac ctcataggct
     2041 gcgagccggt caagttccac ctgtctgcgc aggatgctct caaggtccag cggaagcgga
     2101 gcatcccggt attcggccag cagttccttc cggtaacccg tgaagccgta atcaatgcag
     2161 tccacctcgt ccggccgggc cgacttggaa taaaacacca cgcggccgtt tgcgatgcgg
     2221 acattggatt tgtcaaacag gccgcgattg cggtacacgc tcatgatggc cggcgtgccg
     2281 gcctgttcaa atgcggccgc catggcgcgg tagtccatcg ggagatagga gtctccatag
     2341 aggacgaaga aggtgtcctc cagcaggtcc agggcattca gcagggcgcc gccggtgccc
     2401 atcagcgccc gcgggtcttc aaaggaatat ttcacccgca gcccccatcg gga
//
LOCUS       DAOQWR010000225         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_49903_length_2385_cov_4.695708, whole genome shotgun sequence.
ACCESSION   DAOQWR010000225 DAOQWR010000000
VERSION     DAOQWR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49903_length_2385_cov_4.695708"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKK36_11810"
     CDS             <1..1394
                     /locus_tag="PKK36_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNR95281.1"
                     /translation="PFFCWVHYFDPHKPYHEHRELFGDRYAGRPYEAEIEYMDQQIGR
                     ILKTLEEAGRLDDTLIVAVGDHGESLGEHNELYHGNTLYNSGLHVPLIFRLPGRIFAG
                     KRFAERVSLERIFPTVLELMEIRNRKNPLSLAGPVSGAEQVLPAPIHLETDEPWRQYG
                     WHPLRGLIEGDLKYIRSPHAELYNVRSDPRELNNLAGERPDETARMETALLELESGMT
                     ELTAVSAVLSASDEQILTSLGYTAGASSASPTAPPENLPDVKDMLPLLGKTMHAKSLL
                     GENRPADAARLLEEVLSQDAGNASFHFLYAEALHDAGRCSAAVDVLEHLMQNQEMLLT
                     APLRLDSLTLLGKCFYSMNRLEESAAVLREALRIAPDSTVALNGLAWVLATTPSSTPA
                     DHTEAVRLAEEAVRLRGGNNPSHLDTLSVALASAGRRQEARIAAEKALKLAEEQGNRP
                     LAAEIRARLKKFQ"
     gene            complement(1464..>2385)
                     /locus_tag="PKK36_11815"
     CDS             complement(1464..>2385)
                     /locus_tag="PKK36_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR95282.1"
                     /translation="LKNLPGSFSLLLALAVPGFVISLRHKSLRPGLVLAALFTIFHLA
                     SLIPFVYSSQYRIAMLPLMILFAAAALHDIGIKIIERNTRRALPAILGATGIYLAGAW
                     GANAPSTLPQWHYQQAFALERLGRPDEAARHYQAMIELDPGSAWAHGALGRYHAERGR
                     IREAIVHFSQALKLEPDYPKARSQLGSALYLAGRKSEGISELRRVLMKDPDDAASLNA
                     LAWILASDSDPSIRNPAEAVRLAERACELTGRKSAALLDTLAAALAALGDTSRAVQTA
                     EEALAAAQNNGSSELRAGILHRLENYRQKQ"
BASE COUNT          481 a          706 c          735 g          463 t
ORIGIN      
        1 agcctttttt ctgctgggtg cattactttg atccccacaa accctatcat gagcatcgtg
       61 agcttttcgg ggaccgctat gccggccggc cctatgaagc tgaaatcgaa tacatggatc
      121 agcagatagg tcgtatcctg aagacccttg aagaagcggg ccgactggac gacacgctca
      181 ttgtcgccgt gggtgatcac ggcgaaagcc tcggcgagca caatgaactc taccatggca
      241 acacgcttta caattcggga ctgcatgtgc cgctgatctt ccgactgcca ggaaggattt
      301 tcgccgggaa acgctttgcg gaaagggtgt cgctggagcg gatattccca accgtgctgg
      361 aactcatgga gatcaggaac aggaagaatc cattgagtct ggccggcccc gtttcgggcg
      421 cggaacaggt tcttccggcc ccgattcatt tggaaaccga tgaaccctgg cggcagtacg
      481 gctggcatcc gctgcggggg ctgatcgagg gcgatttgaa gtacatccgt tcgccgcacg
      541 ctgagctgta taatgtccgg tctgatcccc gggaactgaa caatctggcc ggggagcgtc
      601 cggatgaaac ggcccgaatg gaaacagcgc tgctggaact ggaatccgga atgaccgaac
      661 tcaccgcggt tagcgcagtc ctgtccgcgt ccgatgagca gatcctgacc agtctcggct
      721 atacggccgg agcgtccagc gcttcgccga ccgctccgcc ggagaatctg ccggatgtca
      781 aggacatgct cccgctgctc ggaaaaacca tgcatgcaaa atccctgctg ggggagaacc
      841 gtcccgcaga cgccgcgcgg cttcttgaag aggtgctctc gcaggatgca ggaaacgcct
      901 ccttccattt tctttacgct gaagcgctcc atgatgcggg gcgctgttca gccgccgtgg
      961 atgtgctgga gcacttgatg cagaatcagg aaatgctcct tacggcgcct ctgcggctgg
     1021 acagtctgac tctgctgggg aaatgttttt acagcatgaa ccgcctggag gaatccgcgg
     1081 ccgtcctgcg cgaggcgctg cggattgctc cggattcaac cgttgccctc aacggtctgg
     1141 cgtgggtgct ggccacaacg ccgtcttcaa ccccggctga tcacacggag gccgttcgcc
     1201 ttgcggaaga ggccgttcgc ctgcgcgggg gtaataatcc gtcgcacctc gataccttgt
     1261 ccgtggcgct ggcgtccgcc ggccgccgtc aggaggcccg catagccgct gaaaaggccc
     1321 tcaaactggc cgaagagcag gggaacaggc cgctggctgc tgaaatccgc gcccgcctca
     1381 aaaagttcca atgattggaa cttttcgaga aaaaacttcc aatggttgga actttttgcc
     1441 ccagaacttc caatgattgg aagttattgc ttctgccggt agttctcaag ccgatgcaga
     1501 atcccggccc gcagctcgga gcttccattg ttctgtgccg cggccagcgc ttcttcagcg
     1561 gtttgaacgg cccgcgaggt gtctccgagg gcggccagag ccgccgcgag agtatccagc
     1621 agggccgccg atttccggcc cgtcagctcg caggcgcgtt ccgcgagccg cacggcttcg
     1681 gcggggttgc ggatggaggg gtcgctgtcc gaggccagga tccacgccag ggcattcagc
     1741 gaagccgcat catccggatc tttcatcaag acccttctga gttctgaaat gccttcggat
     1801 ttccggcctg ccagatacag cgcggagccc agctggctgc gggcctttgg atagtccggc
     1861 tccagtttga gcgcctgtga aaaatgaaca atcgcttcgc ggatgcgtcc ccgttcggca
     1921 tggtaacggc ccagcgcgcc gtgcgcccat gcgctgcccg gatccagttc aatcatggcc
     1981 tgataatgcc gggcggcttc atccggccgg ccgaggcgtt caagggcaaa cgcctgctga
     2041 tagtgccact gcggaagggt ggagggggca tttgcacccc acgcgcccgc caaatagatt
     2101 cccgtcgcgc ccagaatggc cggaagggcc ctgcgggtgt tccgctcaat gatcttgatt
     2161 ccgatgtcat gcagcgccgc ggccgcgaac agaatcataa gcggaagcat ggcgatgcgg
     2221 tactggctcg aatagacaaa gggaatcagg gaggccagat ggaagatggt gaacagcgcg
     2281 gcgagaacga gtcccggcct caacgacttg tgtctgagac tgatcacgaa acccggaacg
     2341 gccagcgcca gaagcaggga gaagctgccc ggcaggtttt tcagc
//
LOCUS       DAOQWR010000226         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_49941_length_2384_cov_6.426363, whole genome shotgun sequence.
ACCESSION   DAOQWR010000226 DAOQWR010000000
VERSION     DAOQWR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49941_length_2384_cov_6.426363"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="recN"
                     /locus_tag="PKK36_11820"
     CDS             complement(<1..1656)
                     /gene="recN"
                     /locus_tag="PKK36_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNR95283.1"
                     /translation="MLATLRVKNMALAEKIEVEFGPGLNIITGETGAGKSILMGALAI
                     LMGERADKSLIRAGEDACGAEAVFHLADASEIDAILEQNALPPCADGELILRRLVRAS
                     GPGQNLVNDSPVTLPVLKSIGEFLVDMHGPHDHQSLLRTETQLAMLDAFGRLWDQRLK
                     YETVYDELRALEKQRDELTAAEDVSGQIEMLNWRIREIREAALQEGEEEQIETEHQVL
                     GHAGRIQELAGAVVQALTGADEGSAFDVLSGSRRALEELQRLLPEAETWAAETDDILR
                     RMQELDMNIRRAAEDIEASPARLAWLDERLAVYHKLKRKYGTDVGGLLALQRESEEKL
                     KMLQTREERLGQIQAEIAACTARLEKQALALREKRVAASTRLSSLIEKELKALGLAHG
                     EFVITFREAQPSASGMDEVEFEFAPNLGEPVSPMRTIASSGEISRVMLAVKKVLADHD
                     RIPVLVFDEIDSNIGGEIGSAVGRKLREVASGHQVLCITHLPQVAVFGARHYAVSKQV
                     RSGRTVSSVLPLDGESRVEEVARMLGGRELTSVTLKHAREMLAG"
     gene            complement(1673..>2384)
                     /locus_tag="PKK36_11825"
     CDS             complement(1673..>2384)
                     /locus_tag="PKK36_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95284.1"
                     /translation="SDEMLESASAAWTAVSDFLDGHLDIGVRMTKHELESSSRPPENA
                     FLGNITKLDGDEDYNMNLFFDVMFIKYVGIEYTSYELSADTYNWNTGTSDGSMEISGP
                     IWSIIGRFPNQTRFTPYAGIGKASVDASFSNDRWWNLGYPTEAYWLDAGSPSTGYRNI
                     RREITVDGDDQNVMFFGCAIRITDWLSADIYYRTMDITLDAHYHRTGAYEQDDAQDLD
                     GEFTLDHTTLGFGLKYVF"
BASE COUNT          432 a          774 c          692 g          486 t
ORIGIN      
        1 cccggccagc atctcgcggg catgcttgag cgtgacgctg gtcagctcgc ggccgcccag
       61 catgcgcgcg acttcctcca cgcggctttc gccgtccagc gggagcacgc tggaaaccgt
      121 gcggccggac cggacctgtt tggaaaccgc ataatgccgg gcgccgaaaa ccgcgacctg
      181 cggcagatgc gtaatgcaca gcacctgatg gccggaagcc acttcgcgca gcttgcggcc
      241 caccgcgctg ccgatctcgc ccccgatgtt gctgtcgatt tcatcaaaca ccagcaccgg
      301 gatgcggtcg tgatccgcaa gcaccttctt gacggccagc atcacgcgcg aaatctctcc
      361 gctggaggca atggtcctca tcggactcac gggctccccg aggttcgggg cgaattcaaa
      421 ttcgacttcg tccatgcccg aggcggaggg ctgggcttca cggaacgtga tcacaaattc
      481 cccgtgcgca agacccagcg ctttcagctc cttttcgatc agggatgaaa gccgggtgga
      541 cgcggccacc cgcttctccc gaagcgcgag agcctgcttt tcgagccggg ccgtgcaggc
      601 cgcgatttcc gcctgaattt ggcccagacg ctcttccctc gtctggagca ttttcagttt
      661 ctcctccgac tcccgctgca gcgccagcag gccgcccaca tcggttccgt atttccgctt
      721 cagtttatga tagaccgcaa ggcgctcgtc cagccaggcc aggcgcgcgg gactggcttc
      781 gatatcctcc gcggcacggc ggatattcat gtccagctcc tgcatgcggc gcagaatgtc
      841 atcggtctcg gcggcccagg tttccgcttc cggcagcagc cgctggagtt cctccagcgc
      901 ccggcgggag cctgagagca catcaaaggc cgagccctcg tccgccccgg tcagggcctg
      961 cacgacagcg ccggccagtt cctgaatgcg gcccgcatgg cccagcacct gatgttcggt
     1021 ttcgatctgc tcctcttcgc cctcctgcag cgccgcctcc ctgatttccc tgatgcgcca
     1081 gttcagcatt tcgatctgcc cggagacatc ttcggcagcg gtcagttcat cccgctgctt
     1141 ctcgagcgcg cgcagttcgt catagaccgt ttcatatttc agccgctggt cccagagccg
     1201 gccaaacgcg tccagcatcg caagctgggt ttcggttctc agcagcgact gatgatcatg
     1261 gggcccgtgc atatccacaa ggaattcgcc aatgctcttg agcacgggaa gcgtcacggg
     1321 cgagtcgttc acaaggttct gccccggccc ggaggcccgg accagacggc ggagaatcag
     1381 ctccccgtcc gcgcagggag gcagcgcgtt ctgttcaagg atggcgtcaa tctcggaggc
     1441 gtccgccaga tggaacaccg cttcggcccc gcaggcgtcc tcgccggccc gaatcaggga
     1501 tttgtcggcg cgctcgccca tcagaatggc cagcgccccc atcagaatgg attttcccgc
     1561 gccggtctcc cccgtgatga tgttcagccc cgggccgaat tccacttcga tcttctcggc
     1621 cagcgccatg tttttaacgc gaagtgtggc cagcatggat caaagccttt tttcagaaaa
     1681 catacttgag gccgaagccc agcgtggtgt gatccagcgt gaattcgccg tccaagtcct
     1741 gcgcatcgtc ctgctcatac gcgccggtgc ggtggtagtg cgcgtcgagc gtgatgtcca
     1801 tggtccggta gtaaatgtcg gccgaaagcc agtcggtaat gcgaatggcg cacccgaaga
     1861 acatgacgtt ctggtcatcg ccgtcaacgg taatttcccg ccggatgttg cggtagccgg
     1921 tggacgggct gccggcatca agccagtaag cctcggtcgg atatcccaga ttccaccacc
     1981 ggtcgttcga aaaactggcg tccacggaag ccttcccgat tcccgcatac ggggtgaaac
     2041 gggtctgatt cgggaagcgg ccgatgatgg accagattgg cccgctgatt tccatggagc
     2101 cgtcgctggt cccggtgttc cagttgtagg tgtcggctga aagttcatag ctggtgtatt
     2161 caatgcccac gtatttgatg aacatgacat cgaaaaacag attcatgttg taatcttcat
     2221 cgccgtccag ctttgtgata tttccgagaa aagcgttttc gggcggccgg gaactgctct
     2281 ccagttcatg cttcgtcatg cgcacgccaa tatccagatg gccgtccagg aaatcggaaa
     2341 cagcggtcca cgccgcggag gccgattcca gcatttcgtc cgaa
//
LOCUS       DAOQWR010000227         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_51336_length_2342_cov_7.626148, whole genome shotgun sequence.
ACCESSION   DAOQWR010000227 DAOQWR010000000
VERSION     DAOQWR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51336_length_2342_cov_7.626148"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PKK36_11830"
     CDS             complement(<1..1535)
                     /locus_tag="PKK36_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95285.1"
                     /translation="MRMKHTSRIVVGIGLAFVVCVLAVIAADVKQTDRLPIDREVYKE
                     FMSTEVVDIYGLEQFFVDQQDVYLPIIPPDPDFILHQSASPYVLPFKWENFPAEFSGN
                     LVVSYVNSAPVYEVTILEDFNTRETVFLNAKGEKLFSLPPLPSYDPFSYLRTIFASLY
                     DGRYNADQISYWQRFYDPARIQIKVKLLPTEYVEPYLYVSALIENTAQRNLLSRKGRI
                     VKLRSPEAETNIVFESVSRTNGGIRMAIGYPNTFTNRLDVFTCNDLLQYVWTFAKKEL
                     STSGTNEIAWVDTNYWIASGPPIRLYAAGNADKDTDGDGYSDAREIMVYKTDFTNSAS
                     RPVKVSGTVSYSGIETGTIYVLSVTASDSWSTAQAVALPGPGAYSNDIGNDQSYWFKA
                     FRDANTSYDMDEWEPWGIYSNAALTVTTDISGINIVMQDQASIWGAVDYTGSETGDIH
                     VIAVSTSNSWDTTYQCVIPWIQGQQSQTGGDIYVTFPADYMISGLPSSNYWIRAFVDT
                     DTN"
BASE COUNT          511 a          637 c          651 g          543 t
ORIGIN      
        1 tcgtttgtgt ccgtatctac aaacgcccga atccagtagt ttgaagacgg caggccgcta
       61 atcatgtaat ccgccgggaa tgtcacgtag atatcgccgc cggtctggct ctgctgtccc
      121 tgtatccacg ggattacaca ttgataggtt gtgtcccagc tgttggaagt tgaaaccgct
      181 atgacatgga tatctccagt ttcacttccc gtataatcca ccgcccccca gatgctcgcc
      241 tgatcctgca tcacgatgtt gattcctgat atatcggtgg tcacggtcaa agcggcgtta
      301 ctgtaaatcc cccacggctc ccattcgtcc atatcatagc tggtgttcgc gtcacggaac
      361 gccttgaacc agtagctttg atcgtttccg atgtcgttgg aataggctcc gggaccgggc
      421 agcgctaccg cttgggctgt tgaccagctg tcggatgccg tgacgctcag gacatagatc
      481 gttccggtct cgattccgct gtagctcacg gttcccgaca ctttcacggg ccgggaagcc
      541 gaattcgtga agtctgtttt ataaaccatg atctcgcggg cgtccgaata cccgtcgccg
      601 tcggtatcct tgtcagcgtt gcctgcggca taaaggcgaa tcggcgggcc gctcgcaatc
      661 cagtagttgg tgtccaccca tgcgatctca ttggtgccac tggtggacag ttcttttttc
      721 gcaaaggtcc agacgtactg cagaagatcg ttgcaggtga acacatccag ccggttcgta
      781 aaggtgttcg gatacccgat tgccatccga atcccgccat tggtccggga aactgattca
      841 aagacgatgt tggtttcggc ttcgggcgag cggagtttta caattcggcc cttcctgctc
      901 aaaagatttc gctgggctgt gttctcaatc agggcggata cataaagata aggttcaacg
      961 tattcggtcg gcagcagctt cacctttatc tgaatccggg ccggatcgta aaagcgctgc
     1021 cagtaggata tctggtctgc gttgtagcgc ccatcgtaca gactggcaaa gatggttcgc
     1081 agataagaaa acgggtcgta actgggcagc ggcgggagtg aaaacagttt ttcgcctttc
     1141 gcgttcagga ataccgtttc gcgggtgttg aaatcttcaa ggatcgtgac ttcataaacc
     1201 ggcgcgctgt tcacatagga gacgacgagg tttccgctga attcagcggg gaagttttcc
     1261 catttgaagg gcagcacgta tggactggcg gactgatgga gtatgaagtc gggatcgggc
     1321 ggaatgatcg gcagataaac gtcctgctga tcaacaaaga actgctcaag gccgtagatg
     1381 tccaccactt ccgtggacat gaattccttg tagacttcac ggtcaatggg gagcctgtcg
     1441 gtctgtttca catctgccgc tatgacggcc agaacgcaca caacaaacgc aagcccgatt
     1501 ccgactacaa tcctgctggt gtgtttcatc ctcatttcct tccttgattg cgtttcctct
     1561 taaagctctg gtcgcgcact ccgcatccgc acaaagaggg cgcggacatc cgccacgcac
     1621 tcttggaggg actggcgtcc ccttggagga acatgaacag gagccgcgcc caatcgggcg
     1681 cgtgctcgcc tgttcgctcc tccgtacaaa gtgccagttt tccaagagaa cgaatcttgc
     1741 gattacgcta tattcaaatg tcttttaaaa gttggatatt cttacgtaac aagaacggat
     1801 aaatacctca ctttcaaaca agtcattgtt cgcaccgccc cggctgtctt gcaagatatt
     1861 tcttttgaaa aagaaagcgt ccgatattca agagcagcca cggagggcca aaggcccgac
     1921 cgccagcaaa ggccagcccg aagggcgcag gccgaagcgg tgccgggcgc atttttttcc
     1981 aatgcgaccg gcaggccatg ccgccggatt cctgccaatt accccgcctt tcccgtaatc
     2041 gggatggacg gggttattgg cccggcggct ccgggcagac gcatttgaag tgacggggtt
     2101 cgatggcaga gttgaccgga cgcaggggac catagccctg cgggaggtca acgacgtccg
     2161 agaaccacgc acttgaaagg cggcattaag cgaagaccgg aggtctgagc gctctggaga
     2221 gcggcggcag ccgtgcgcag cacggcccga aggcgtgaaa ggtttctgcc gattccgcat
     2281 cctctcctct tcgcgcgggc aggggccgcc ccgtttcggg gcggttttca gaaaaggcag
     2341 aa
//
LOCUS       DAOQWR010000228         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_51997_length_2323_cov_4.421076, whole genome shotgun sequence.
ACCESSION   DAOQWR010000228 DAOQWR010000000
VERSION     DAOQWR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51997_length_2323_cov_4.421076"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKK36_11835"
     CDS             <1..244
                     /locus_tag="PKK36_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95286.1"
                     /translation="TNSEMQISMQTLAATGTAYELYSRREYTFEQALSPTGSWSGIFG
                     WQGLTGDGQRLVLTNSPPEDRGFYRGYIQLVPLRQP"
     gene            484..1746
                     /gene="icd"
                     /locus_tag="PKK36_11840"
     CDS             484..1746
                     /gene="icd"
                     /locus_tag="PKK36_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNR95287.1"
                     /translation="MGFEKIKVPMTGEKITMGPDGKLVVPDFPILPFIEGDGIGPDIT
                     RAAMIAWNAAVEKAYRGERRISWMEIYAGEKSVSVYGEGVWLPDETIEAVREYLVAIK
                     GPLTTPVGGGIRSLNVALRQLLDLYVCLRPVRWFAGVPSPVRQPELTDMVIFRENSED
                     IYAGIEWAAGTPEVKKVIEFLQKEMGVKKIRFPNTSGIGIKPVSEEGTRRLVKAAIEY
                     AIENSRPSVTLVHKGNIMKFTEGGFRDWGYQTAKDLFGAVPLDGGPWHKLKSPKSGKD
                     IIIKDVIADAMLQQILTRPADYDVIATLNLNGDYLSDALAACVGGIGIAPGANINYDT
                     GHAVFEATHGTAPKYAGKDQVNPGSLILSGEMMLRYMGWTEAADLILESMSKTISQKR
                     VTYDFHRLMENATLLKCSEFGQALAENM"
     gene            1757..2251
                     /gene="rfaE2"
                     /locus_tag="PKK36_11845"
     CDS             1757..2251
                     /gene="rfaE2"
                     /locus_tag="PKK36_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNR95288.1"
                     /translation="MKLASAKILSREEMKQERARLKAAGKKVSFTNGCFDIIHAGHVD
                     YLQWARNQADALIVGLNSDASVRRNKGDKRPIVDEHDRAYVLAALACVDYVVLFDEDE
                     PAQLIADIVPDVLVKGEDWAHYVSGRETVEAAGGRVALAPLVEGRSTSNLISKIVDVY
                     GGTD"
BASE COUNT          509 a          696 c          654 g          464 t
ORIGIN      
        1 gacgaacagt gaaatgcaaa tatccatgca gaccctcgcg gcaacgggaa cggcatatga
       61 actctacagc cgcagagaat acacctttga gcaggcgctc agccccaccg gatcctggag
      121 cggaattttc ggctggcagg gcttaaccgg tgacggacag cggctggttc tcaccaactc
      181 gccgccggag gaccggggct tctaccgcgg ttacatacag cttgttcccc tgcgccagcc
      241 ctgatcgcgc ccttgcacct ccaattttcc ggatagatca ttttttaaat ttttttggga
      301 atttttgcat tttttgacgt ttttttgcca ttttttgcat tttttgacgt ttttaggccg
      361 tttttgaccg ttttcgggcc atttttgacc gtttttcgtt gatttttcgc aaatatttgc
      421 aggtcgacag aagaggccca aaaacgtata aaaatgcttt ttcctaatga agaaaggcag
      481 tttatgggtt ttgaaaagat caaggttccg atgacaggcg aaaagatcac gatgggtccg
      541 gacggcaaac tggtcgtccc ggattttccc atcctgccgt ttatcgaagg cgacggcatc
      601 gggccggaca tcacccgcgc cgcgatgatc gcctggaacg ccgccgttga aaaagcctac
      661 cgcggcgagc gccggatttc gtggatggaa atctatgccg gcgaaaaatc ggtgagtgtc
      721 tacggcgaag gcgtctggct tcccgatgaa accatcgaag ccgtccgcga atatctggtc
      781 gcaatcaagg gtccgctgac cacacccgtc ggcggcggca tccgcagcct gaacgtcgcc
      841 ctgcgccagc ttctcgatct ttatgtctgc ctccgccccg tgcgctggtt cgcaggcgtc
      901 ccctcgcccg tccggcagcc cgagctgacc gatatggtaa tcttccgcga gaattccgaa
      961 gacatctatg ccggcatcga atgggccgcc ggaaccccgg aagtgaagaa ggtgatcgag
     1021 ttcctgcaga aggaaatggg cgtgaagaaa atccgcttcc cgaacacctc cggcatcggg
     1081 atcaagcccg tctccgaaga aggcacgcgc aggctggtga aggccgccat tgagtacgcc
     1141 attgaaaaca gccggccctc cgtgaccctc gtccacaagg gcaacatcat gaagttcacc
     1201 gaaggcggct tccgcgactg gggctaccag accgcgaagg atctcttcgg cgccgtgccg
     1261 ctggacggcg gcccgtggca caagctgaag agccccaaaa gcggcaagga tatcatcatc
     1321 aaggatgtca tcgccgacgc catgctccag cagatcctga cgcgtccggc cgactacgat
     1381 gtcatcgcca cgctcaacct caacggcgac tatctgtccg acgcgctcgc cgcctgcgtc
     1441 ggcggaatcg gcatcgctcc cggcgccaac atcaactacg acaccggcca cgcggtcttc
     1501 gaggccaccc acggcaccgc gcccaagtac gcgggcaagg accaggtgaa tcccggatcg
     1561 ctgattctct ccggcgaaat gatgctccgc tacatgggct ggaccgaggc ggccgacctc
     1621 attctggagt ccatgagcaa gaccatttcc cagaagcgcg tgacttatga cttccaccgc
     1681 ctgatggaaa atgcgacgct tttgaaatgc agcgaattcg gacaggccct tgccgaaaat
     1741 atgtaatgag gttctcgtga aacttgcctc agccaaaatc ctgtcgcgcg aagaaatgaa
     1801 gcaggaacgc gcgcgcctga aggcggccgg gaaaaaggtt tcgttcacca acggatgttt
     1861 cgacatcatt cacgcgggcc atgtggacta tctccagtgg gcccgcaacc aggccgatgc
     1921 cctcattgtc ggcctcaact ccgacgcctc cgtccgtcgc aacaagggcg acaagcgtcc
     1981 catcgtggac gaacacgacc gcgcctatgt gctggccgcg ctggcctgtg tggactacgt
     2041 ggtgcttttc gacgaagacg agcccgcaca gctcatcgcc gatattgttc ccgatgtgct
     2101 ggtgaagggc gaagactggg cccattacgt cagcgggcgc gaaacagtcg aggccgccgg
     2161 cggccgcgtg gccctggccc cgctggtgga aggacgctcg accagcaacc tgatcagcaa
     2221 aattgttgat gtgtacggag gcacggactg acatggcaaa gatcatcgtt accggcggag
     2281 cgggattcat cggaagcaac cttatcgcgg aactcaacgc gcg
//
LOCUS       DAOQWR010000229         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_53776_length_2273_cov_2.547791, whole genome shotgun sequence.
ACCESSION   DAOQWR010000229 DAOQWR010000000
VERSION     DAOQWR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53776_length_2273_cov_2.547791"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKK36_11850"
     CDS             <1..513
                     /locus_tag="PKK36_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95289.1"
                     /translation="MLGKSAYPNPESLMQKEYRVAKKLSEYADIQNVRIACGDSGVIA
                     YFTGAKFLDTVGLNDGFIARNRDIDLLTDYFFGEKPTLVFAMANKDHTWIDYGHGPLL
                     KRTDWMRDPRWDDYIYAGTITTSGGMYDLHLMLRKDYEQMEELADFLAARIADRMYDP
                     FPLPIGTYVP"
     gene            530..>2273
                     /locus_tag="PKK36_11855"
     CDS             530..>2273
                     /locus_tag="PKK36_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95290.1"
                     /translation="MSKSSNNCPRWLTSVPAIFLAVLLTPALLASLYVLMARGLMPGG
                     AGVVLAGMVCVVLLGGVVRLVTRDLGPRAHAAAVGAVIAGGALVRFAVVLGCRGFVQL
                     EDRGIFIRFVELLEQGGLNAECLSVLSEWYEYNVWIHRAFPLAYPLRWLFGEQHVLAF
                     QLVSAMLSSAAMVVFYALASELLQEKGRRAALAVYAFFPLRLWNVLEYSHRIQIEILI
                     LGVLLLVVKQLRGCTRWVPVALRGAAVGLILFLLNLQRGVDLLLLLFFCAVVFCGQFR
                     LPSLSSKFRLASGLVLIPVMIYLPLVLSFDSWCGKYDLKQMDSGFSGFMARGWSLETL
                     GEFDGKYEQIDKATPVWLKGKVMRAVVFSQLKYHPRLVFSRLLPAKMAKYFLVGSATG
                     MEQGLDQAGLMIPARIFTGQRVVFAPIFLLLVILSSVRMSAGGGEPVVWSSLIMFPLL
                     ACGVFVVLGESSPYYSFYVHAVLVLVAGRLWEPLPETLQAQRRLVRRELVRGCLLFAS
                     LYAVAGIAAPALAGLLPPEFTFHDISRAEITASWTDERMSPRQVSPSLEPYLQRISLP
                     VRPYSAGETVCARLP"
BASE COUNT          387 a          563 c          709 g          614 t
ORIGIN      
        1 atgctgggta aaagcgcgta tccgaacccc gagtcattga tgcagaagga gtatcgcgtt
       61 gcaaagaagc tctctgaata cgcggacata cagaatgtgc gcatcgcctg tggtgattcc
      121 ggagtgattg catatttcac cggggcgaag tttctggata cggtggggct gaacgacgga
      181 ttcattgccc gcaacaggga tatagatctt ctgacggact attttttcgg agagaagccc
      241 acgctggtgt tcgccatggc aaacaaggat catacgtgga ttgattacgg gcatggccct
      301 ctcctgaaac ggacggactg gatgagggat ccccgatggg acgattatat ctacgcgggc
      361 acgataacaa cgagcggcgg tatgtacgat ctccacctga tgcttcgcaa agattatgag
      421 cagatggagg aattggcgga ttttctggcg gcgagaattg ctgatcggat gtatgatccg
      481 ttcccgctgc ccattggcac ctatgttccc tgaagttgtc tgaaacagaa tgagcaaatc
      541 atcgaataat tgtccgcgct ggctgacaag tgttccggcg atctttctgg cggtgctgct
      601 gacccccgct ttgctggctt cgctctatgt gctgatggcc cgtggtctca tgccgggcgg
      661 cgcgggtgtt gttctggccg gcatggtctg tgttgtgctt ctggggggtg ttgtccggct
      721 cgttacgcgg gacttgggcc cacgggcgca tgcggcggcg gtgggggcgg tgatcgcggg
      781 aggagccctg gtgcggttcg cggtggtgct tgggtgccgg gggttcgtgc aacttgagga
      841 ccggggtatt ttcatccgtt ttgttgaact cctggaacag ggagggttga atgcagaatg
      901 cctctccgtg ctgagcgaat ggtatgaata caacgtctgg attcatcggg catttccgct
      961 ggcctacccg ttgcgatggc ttttcggaga gcagcatgta ctggcgttcc agttggtcag
     1021 tgcgatgctt tcatcagccg ccatggttgt tttttatgct ctggcatccg aacttttgca
     1081 ggagaaaggc cgtcgtgcgg ctcttgcggt ctacgcattc tttcctctgc ggttatggaa
     1141 tgtgctggaa tattcgcatc gaatacaaat tgaaattctt attcttggag ttctgctgct
     1201 ggtggtaaag cagctgcggg gctgtacccg atgggtgccg gtggcgctgc gcggtgcggc
     1261 cgtcggattg atccttttcc ttctgaatct tcagagaggc gtggatttgc tgcttctgct
     1321 gttcttctgt gcggtggtct tttgcggaca attccgtctt ccatcccttt cttcaaaatt
     1381 caggctggct tccgggctgg tgctgatacc ggtcatgatt tatctgccac tcgtcttgtc
     1441 ctttgacagc tggtgcggga aatatgatct gaagcagatg gattcaggtt tctccggatt
     1501 catggcgcgt ggatggagcc tggaaaccct gggagagttt gacgggaagt acgagcagat
     1561 agacaaggcc acgccggttt ggctgaaggg taaagtgatg cgggctgtgg tcttctccca
     1621 gttgaaatat catcccaggc tggttttttc ccggctgctc cctgccaaga tggctaaata
     1681 ctttcttgtg ggttctgcga caggaatgga gcaggggctg gaccaggcgg gtttgatgat
     1741 tcctgcccgg atatttaccg gacagcgcgt tgtattcgcg cccatcttcc ttctcctggt
     1801 gattctttca tctgtccgga tgtcggccgg ggggggcgaa ccagtggtct ggtcttcgct
     1861 gatcatgttc ccgctgctgg cctgcggcgt ttttgtggtg ctgggcgaaa gttcgccgta
     1921 ttactcgttc tatgtccacg cagtccttgt gcttgttgcg gggcgtttgt gggaaccgct
     1981 tccagaaaca ctccaggcac agcggcggct cgtgcgccgg gaactggtgc ggggctgttt
     2041 actcttcgcg tctttgtatg cggtcgccgg aatcgctgct cccgcgctgg ccggactcct
     2101 tcccccggaa tttacatttc atgatatctc acgggctgaa atcacggcct cgtggactga
     2161 tgagcgcatg tctccccggc aggtcagccc ttcgctggag ccctaccttc agcgcatttc
     2221 gctgcctgtc cgtccttatt cggcagggga aacggtctgt gcgcgactgc ctc
//
LOCUS       DAOQWR010000230         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_53829_length_2271_cov_5.013989, whole genome shotgun sequence.
ACCESSION   DAOQWR010000230 DAOQWR010000000
VERSION     DAOQWR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53829_length_2271_cov_5.013989"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PKK36_11860"
     CDS             <1..965
                     /locus_tag="PKK36_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HNR95291.1"
                     /translation="EPALRSTKIIAEAWDAAGVYQVGSFPNERWSEWNGRFRDDVRRF
                     WRGEPDMLRAFAARMAGSPDLYNMKNQAPQKSINYVCCHDGFTLNDLVSYEHKHNEAN
                     GEENRDGDNHNHSSSWGVEGETGDSTILSVRRRQQKNMLATVLLARGVPMFQAGDEFG
                     RTQKGNNNAYCQDNEISWVNWHLLHEHADLHAFVRQLIAFRKKHPALNSILFPNHANG
                     SGSPDALYWYGPDGKDPDWEHGQAVACVLDGSSKATGVQDDDPILLLINASDKEVIFT
                     LPLLAGGRWKIEFSTEDAAPSADHETGTMTLGSRALCALTGVPG"
     gene            962..1849
                     /locus_tag="PKK36_11865"
     CDS             962..1849
                     /locus_tag="PKK36_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNR95292.1"
                     /translation="MRRFFPLVLLLVSIFGAPACFAAKKWRVYPNCTLVENPANDGDS
                     FHVKYNRRRYLFRLYFVDAPESDNSLPERVREQADYFGLSEKDTVQLGKEAAKFTQKF
                     LANGFTAHSKLKDALGRSKKDRDYAMIQVGEKYLCEALVENGYARIFGLTEDELPDGK
                     SGKTLLWRLKSLEREAQKNKLGGWAPRLTRMEEMQQRALPPAAVLPPAVVPAPAPVPT
                     PAAPDTVKPALEQQTITLENSINVYSLLDPGKLMGILKAGADVQILGEEPRDMVRIRF
                     KTPAGKVYEAQCFRRELGL"
     gene            1969..>2271
                     /locus_tag="PKK36_11870"
     CDS             1969..>2271
                     /locus_tag="PKK36_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR95293.1"
                     /translation="MGTTSVQRKVLVVDDHKLLRDMLKTFLEGEYEVLHAETAEEGLR
                     IFRASAPDVVLMDIVMPGESGLDALKTMRNLDDKISVVMMTGFPKLETAEEAISLGA"
BASE COUNT          539 a          667 c          660 g          405 t
ORIGIN      
        1 aggagccggc cctccgcagc accaagatca ttgccgaagc atgggacgcc gccggcgtgt
       61 atcaggtggg gtccttcccg aatgagcgct ggtctgaatg gaacggccgc ttccgggacg
      121 acgtgcgccg cttctggagg ggtgaaccgg acatgctgcg cgcctttgca gcccgcatgg
      181 ccggaagccc cgatctgtac aacatgaaga atcaggctcc gcagaaaagc atcaactatg
      241 tctgctgcca tgacgggttc acgctgaacg accttgtcag ctatgaacac aagcacaatg
      301 aagccaacgg agaagagaac cgcgacggcg acaatcacaa tcattcctcc agctggggtg
      361 ttgaggggga aaccggtgat tccacgattc tttcggtccg tcggcgccag cagaaaaaca
      421 tgctggccac cgtgctgctg gcgcgcggcg tgcccatgtt ccaggccggc gatgaattcg
      481 gccgcacaca gaaaggcaac aacaacgcct actgccagga caacgaaatc tcctgggtga
      541 actggcacct gcttcacgaa catgcggatc tgcatgcctt cgtcaggcag ctgatcgcct
      601 tcaggaaaaa gcatccggcc ctcaacagca tcctcttccc aaaccacgca aacgggagcg
      661 gttcacccga tgccctctac tggtacggac cggacgggaa ggacccggac tgggagcacg
      721 gacaagccgt ggcctgcgtg cttgacggca gcagcaaagc caccggcgtg caggacgatg
      781 acccgatcct tctgctgatc aatgcatcgg ataaggaggt gatcttcacg ctcccgctcc
      841 tcgcgggcgg caggtggaaa attgaattca gcacagagga cgcagcccct tccgctgatc
      901 acgaaaccgg aaccatgacg ctgggcagcc gcgcgctttg cgcactcaca ggagttccgg
      961 gatgaggcgt ttcttcccat tggtgcttct gcttgtttcg atcttcggcg cgccggcctg
     1021 ctttgccgca aagaaatggc gggtctatcc gaactgcaca ctggttgaaa atcccgccaa
     1081 tgacggcgac agcttccacg tgaaatacaa ccgccggcgt tacctcttcc gcctctattt
     1141 cgtggacgct ccggagtcgg acaacagcct gcccgaacgc gtcagggaac aggcggacta
     1201 tttcggactg tctgaaaagg atacggttca actggggaag gaagcggcga agttcaccca
     1261 gaaatttctg gcgaacggct tcaccgccca cagcaagctc aaggatgccc ttggccgaag
     1321 caaaaaggac cgggactatg ccatgatcca ggtcggggaa aagtatctct gcgaggcgct
     1381 ggtggaaaac ggatacgccc ggatattcgg actcacggag gacgaactgc ctgacgggaa
     1441 aagcgggaaa actctgctct ggagactgaa aagccttgaa cgcgaggccc agaaaaacaa
     1501 gctcggagga tgggctccgc ggctgacacg aatggaagag atgcagcagc gggcgcttcc
     1561 gccggcagcc gtgctgccgc ccgcagtggt tcccgctccc gcgccggtgc ccactcccgc
     1621 agcccccgac accgtgaaac cggcgctgga acagcagacc atcacactgg aaaacagcat
     1681 taatgtctac tccctgctcg atccgggcaa actcatgggc attcttaagg cgggcgctga
     1741 tgtgcaaatc ctcggcgaag agccgcgcga catggtccgc atccgtttta aaacccccgc
     1801 cggaaaggtt tacgaagcac aatgcttccg ccgcgagctg ggcctctgaa tcttatcatt
     1861 tctgataaaa acccagtgaa acaagggtct ctctcacttt tgagaagggg tggcacggtt
     1921 cctgcttagt taattaccca gaatggaagc ctcaatgggg agaagaaaat gggaacgacc
     1981 tccgtgcaga gaaaggttct ggttgttgat gaccacaagc tgctccggga catgctgaag
     2041 acctttctcg aaggggaata cgaagttctt catgccgaaa ccgccgagga aggcctgcga
     2101 atcttccgcg ccagcgcacc cgacgtggtc ctgatggata tcgtcatgcc cggcgaaagc
     2161 ggcctggatg cgctcaagac tatgcgcaat ctggacgata aaatcagtgt ggtgatgatg
     2221 accggattcc cgaaactcga aacggccgaa gaggccattt ctctcggggc c
//
LOCUS       DAOQWR010000231         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_54547_length_2252_cov_5.018662, whole genome shotgun sequence.
ACCESSION   DAOQWR010000231 DAOQWR010000000
VERSION     DAOQWR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54547_length_2252_cov_5.018662"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..2037
                     /locus_tag="PKK36_11875"
     CDS             145..2037
                     /locus_tag="PKK36_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR95294.1"
                     /translation="MQPSDEIRSPRRRRRHAVPVSARRGIYDGIILTLLLAPSLAGLF
                     LYGGVRTWSISLLMIFSSAGLLLFFLRPLFAEELREIRLPPGFCAGLLLLVYAAIRIP
                     GSEVSHISRFHFVMLANALLVLLAWTTMGSRGSRWKWIAALLIITVSLILWYALVQQV
                     RGTRMVLNLERPEVYGMRASGTHMCPNHFAHLLEVVMCLCLALIASPAAGGALRLFSG
                     YTLLLAAPVLVLTQSRAGWLGAAAGFVVTLWLILWKRRRKLFYISFIVLPLLVAALGF
                     TLWKTVPLVRDRIEQARQGDVRTQLWSDALRVAKDYPVFGCGPGTYRWIEPKYRAHAS
                     QSWVLYAHNEYLQTLAEYGAAGFVLFAVFILWGLGRLFGVYVRRELRGETEALTAGFL
                     GAAAASLVHAVFDFNFHISANTHILLLLGGLASAELYASGVLQVRRPEGWRMRAFSAA
                     GGLAAVLLMVLSAQALASDVFYRLGEKARGEFQDEKALSSFRKAAALDPGFWQPWMGM
                     GHVYKTRSFWNLDEDARKESAGLALQHYEAAQQRNAHSPEILYGRSKVYSSLGEKEKA
                     IELMRGIVSAHPADAFYLTQLGLELRLNGRLEEALEVFERAARLPEPKDVVRINIKLL
                     REALGR"
BASE COUNT          365 a          653 c          719 g          515 t
ORIGIN      
        1 ccagcccaaa aaggtccggc tgctgcgcgg cggaaaatcc acgatccatg atcttaagca
       61 gatcgaaaag aatcctgaca aggatatctc catcgaggcg gaagacgtga tcgtggttcc
      121 gcgaagcgtt ttctagtgcg gaccatgcag ccgtctgatg aaatccggtc cccccgccgc
      181 cgccgccggc atgcggttcc cgtttctgcc cgccgcggaa tttatgacgg gatcattctg
      241 actcttctgc ttgcgccgtc gcttgcgggc cttttcctct atggcggggt gaggacctgg
      301 tccatttcgc tcctaatgat cttttcatct gccggtctgc tgcttttctt cctgcggccc
      361 ttgttcgcgg aggagctgcg ggagatccgc ctgccgcccg gattctgcgc gggcctgctg
      421 cttctggtct atgctgcgat caggattccg ggttccgagg tcagccatat cagccggttc
      481 cattttgtga tgctggcaaa cgcgctgctg gtcctgctgg cctggaccac catgggctcg
      541 cgcggttccc ggtggaagtg gatcgcggcg ctgctgatca tcacggtatc gctcattctc
      601 tggtatgcgc tggtgcagca ggtgcgggga acacgcatgg tgctgaatct cgagcggccc
      661 gaagtctacg gcatgcgcgc cagcgggacc cacatgtgtc caaatcattt tgcccacctg
      721 ctggaagtgg tcatgtgcct gtgtctcgcg ctgattgctt cacctgccgc cggaggagcg
      781 ctccgcctgt tttcgggcta tacgctgctg ctggcggcgc cggttctggt gctgacacag
      841 tcgcgagccg gctggctcgg cgccgcagcc ggattcgtgg tgacgctttg gctcattctc
      901 tggaaaagga gaagaaaact tttctatatc agtttcatcg tgctcccgct gctggtggcc
      961 gcgctcggat tcacgctgtg gaaaacggtc ccgctggtca gggaccgtat tgagcaggcc
     1021 cggcagggag atgtccgcac ccagctctgg tctgatgcgc ttcgggtcgc gaaggactat
     1081 cccgtgttcg gctgcggccc gggaacctac cggtggatcg agccgaaata tcgcgctcac
     1141 gccagccagt cgtgggtgct ctacgcgcac aatgagtatc tgcagaccct ggcggagtac
     1201 ggcgcggccg gattcgtgct ttttgcggtt ttcattctct gggggctggg ccgtctgttt
     1261 ggtgtttatg tcagacggga gcttcgcggc gaaacagagg ccctgacggc gggatttctg
     1321 ggggcggccg cggccagtct ggtgcatgcc gtgtttgact tcaatttcca catttcggcc
     1381 aacacgcata tcctgcttct gctgggaggc ctggcatcgg ccgagctgta tgcctccggc
     1441 gtgcttcagg tccgtcggcc ggaaggctgg cggatgcggg ccttctctgc cgcagggggg
     1501 ctggccgcgg ttctgctgat ggtcctgtcg gcgcaggcgc tggcgtccga tgtgttctat
     1561 cgcctgggtg aaaaggctcg cggggaattc caggatgaaa aggcgctgag cagcttccgg
     1621 aaggcggcgg ccctggatcc cggattctgg cagccgtgga tggggatggg ccatgtctac
     1681 aagaccagga gtttctggaa tctggatgaa gatgcacgga aagagagcgc cggccttgcg
     1741 cttcagcact atgaggccgc gcagcagcgc aatgcgcatt cgccggagat cctctatggc
     1801 cgaagcaagg tttattcctc actgggagaa aaagaaaaag ccattgaact gatgcgcggc
     1861 atcgtgagcg cccatcctgc tgacgccttt tatctcacgc agcttgggct ggagctgcgc
     1921 ctgaacggcc ggcttgaaga ggcactggaa gtgtttgaac gggccgcgcg tttgccggag
     1981 ccgaaggatg ttgttcggat caacatcaag ctgctgcggg aggcgctggg tcgttagttt
     2041 gcgggaagcc atccggcttc gcccgaaaaa aacatccgtc tctttcattt ttcgcttgcg
     2101 cgccccgcgc cgtatgatat gttcaatgcg tattgaacgt tatgcgcatg cgtgacgggc
     2161 ccaaatcctc ggtcgccttg tggaaccctc cgcgcgggaa tccaccggga cgaagtctgt
     2221 cccgcctccg cttttcccgt ttcccgccat cg
//
LOCUS       DAOQWR010000232         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_56312_length_2207_cov_2.818773, whole genome shotgun sequence.
ACCESSION   DAOQWR010000232 DAOQWR010000000
VERSION     DAOQWR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56312_length_2207_cov_2.818773"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..860)
                     /locus_tag="PKK36_11880"
     CDS             complement(9..860)
                     /locus_tag="PKK36_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNR95295.1"
                     /translation="MNTNVKCRILILLMTGIILSASGVLYGDNILLIGDSISLGYQYR
                     AQLYAGSRHSIVHTMANALHSTYCISYAENWLSGTNRVWDVVHFNVGLHDIFRDPDNS
                     APPRTPVETYQENLVKIVNIIRKDSPCAVIVFALTTPILPQEGRLPEDVEVYNSAATN
                     ILADQNVYINDLCSLMLPHQDEYHLEDGTHFTPDGNQFNAENVVNFIDSIPRPKPCLW
                     ADGHWVKYTAKSNDAYNVYFSTNMVAGHCQLIETNILGQGEVRSFQMGSGGESAGWYW
                     IGATTED"
     gene            complement(844..2007)
                     /gene="neuC"
                     /locus_tag="PKK36_11885"
     CDS             complement(844..2007)
                     /gene="neuC"
                     /locus_tag="PKK36_11885"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNR95296.1"
                     /translation="MKRKICAVTGTRAEYGLLYFLLHGISRSADLELQLIVTGAHLSP
                     EFGLTWREIEKDGFRIDKKVEMLLSADTPSSIAKSMGLGLIGFADALNDLKPDMLLLL
                     GDRYELASAALAALVARVPIAHIHGGETTEGAIDEAIRHSITKMSWLHFVAAEEYRRR
                     VIQMGEDPSRVHLVGGMGVDAIKKTKLLNRQDLEKSMGFRFGKKNLLITFHPVTLESG
                     SAGSQFEELLLALGQLKDTHFIFTAPNADAEGRVISGQIDKFVLSHPNVAVQFVSLGR
                     ERYLSTLQFVDAVVGNSSSGLAEAPTFKTGTINIGDRQKGRLKAESVIDCPPRRDSIL
                     EALNTLYSAEFAKRLAKTENPYGDGDATEKIIKILETINLPDEPKKGFYEHKC"
     gene            complement(2004..>2207)
                     /locus_tag="PKK36_11890"
     CDS             complement(2004..>2207)
                     /locus_tag="PKK36_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNR95297.1"
                     /translation="PSPSERKNIPVARKSIVASGDIAAGEKFTEKNITAKRPGTGISP
                     MKWDDVIGRTAKRAYQTDEMIEL"
BASE COUNT          486 a          634 c          546 g          541 t
ORIGIN      
        1 cgcccctttt agtcttcggt tgttgcgcca atccagtacc agcccgccga ttcgccgccc
       61 gatcccattt gaaacgagcg cacttccccc tgacccaaaa tgtttgtttc gatcagctga
      121 cagtggcccg caaccatgtt cgtgctgaag taaacattgt aggcatcgtt cgacttggcg
      181 gtatatttaa cccaatgccc atccgcccac aaacagggct tcgggcgcgg aattgaatca
      241 atgaaattga ccacattctc cgcgttgaac tgatttccat cgggcgtgaa atgcgtaccg
      301 tcctcgagat gatattcgtc ctgatgcggc agcatgagag aacagaggtc attgatgtac
      361 acattctgat ccgcaaggat gttcgtggcc gcgctgttgt acacctccac atcttccggc
      421 agccggccct cctgcgggag tatgggcgta gtcagggcaa atacaatgac tgcgcatggc
      481 gaatcttttc taatgatatt gacaattttc accagatttt cctgataggt ttccaccggg
      541 gtccgcggag gagcgctgtt gtccggatcc cggaaaatat cgtgcaggcc gacattaaag
      601 tgaaccacat cccacaccct gttcgttccc gagagccagt tctcggcgta cgaaatacag
      661 taagtcgagt gaagagcatt tgccatggtg tgcacgatgc tatgcctgct gccggcatat
      721 agctgagccc tgtactgata acccagcgaa atcgaatctc caataagcag gatgttgtct
      781 ccatacagca caccagatgc cgaaagaata atccccgtca tcagcagtat cagtattcgg
      841 cacttaacat ttgtgttcat agaatccttt cttgggctcg tcaggcaggt tgatcgtctc
      901 aagaatcttt atgatctttt cggtggcatc gccatcccca tacggatttt ccgtcttcgc
      961 cagccgcttc gcgaattccg cggaatacag cgtgttcaag gcttccaata tggaatccct
     1021 ccggggcgga cagtcaataa cactttcggc cttcaaccgg cccttctggc ggtccccgat
     1081 gttgatggtg cccgtcttga aagtcggcgc ttcggccaga ccgctggatg aattgcccac
     1141 caccgcatca acaaactgaa gcgttgagag ataccgctcc cggcccagtg aaacaaattg
     1201 aaccgccaca ttcggatgcg acaatacaaa cttgtctatc tgtcccgaaa taacacggcc
     1261 ttccgcatcg gcattgggcg ctgtaaaaat gaagtgcgtg tccttgagct gacccagcgc
     1321 caggagcagt tcttcgaact gactgcccgc tgatccggat tccagcgtaa ccggatggaa
     1381 cgtgatcagc agatttttct tcccgaatct gaaccccatg cttttttcca gatcctgccg
     1441 gttcagcagc ttcgttttct ttatggcgtc cacccccatg ccgccgacca gatgcacccg
     1501 cgacgggtcc tcccccattt gaatgactct tctgcggtat tcctctgccg ccacaaaatg
     1561 cagccaggac atttttgtaa tcgaatgccg gatcgcctca tcaatggcgc cctcggtggt
     1621 ttctcctccg tggatatgcg caatgggaac gcgggccacg agggcagcca gcgcggcgga
     1681 ggccagttca tagcggtccc ccagcaggag cagcatgtcc ggtttaaggt cattgagtgc
     1741 atctgcaaat ccaatcaggc ccagacccat ggatttggca atggaggacg gcgtgtctgc
     1801 cgaaagcagc atttcgactt tcttatcaat acggaaaccg tccttttcga tttcgcgcca
     1861 ggtcaaaccg aattcaggag aaagatgggc gccggtcaca atgagctgca gttccagatc
     1921 ggcggaccgg gaaattccgt gaagcagaaa atacagcagt ccatattccg cccgggttcc
     1981 cgtcactgcg catatttttc ttttcatagc tcgatcatct cgtctgtttg ataggccctt
     2041 ttcgctgttc gcccgatgac atcatcccac ttcatggggc tgatcccggt tccgggccgt
     2101 ttcgcggtta tgtttttctc ggtaaacttc tctccggcgg caatatcccc cgaggcgaca
     2161 atggacttcc gggccacggg aatatttttt ctctcgctgg gagaggg
//
LOCUS       DAOQWR010000233         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_59193_length_2139_cov_5.295585, whole genome shotgun sequence.
ACCESSION   DAOQWR010000233 DAOQWR010000000
VERSION     DAOQWR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59193_length_2139_cov_5.295585"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2139)
                     /locus_tag="PKK36_11895"
     CDS             complement(<1..>2139)
                     /locus_tag="PKK36_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNR95298.1"
                     /translation="DYPVYELQAALRAAAVPGCRIIIRAPTGSGKSTQIPQMLLDGPG
                     GQIVVLQPRRIAARLLAHRVASERGGDVGGEIGYQVRFENHTGPRTRIRYVTEGVLLR
                     QWIDDPELAAVSTVIFDEFHERHLYGDISLSAALRLQQTSRPDLRIIVMSATLDSARL
                     QEHLAPCRVLESEGRVYPVAVEYLPRAVDFRKTAVWDAAAEAFGRLADGGLEGHVLIF
                     MPGAYEIRKTIEMLQSHPRARGLAVLPLHGQLSPRAQDEAVSESGRPRIIVATNVAET
                     SITIAGVTAVIDSGLARMARYDPHRGIDTLYVEKISCASADQRSGRAGRTGPGRCLRL
                     WTAQEHAQRPLQETPEILRVDLAEVVLMLKSCGIDDVRGFPWLDSPGEKALEQAETLL
                     HDLGALGRDGRLQPMGRRMVRFPVHPRYSRMLLTANDYGCVRQAALIAALTQGPPILL
                     RNAGKPVAEKREELFGDEFSSDFFLLMKAWKYAETKNYSPGACQALGIHGVSARQVQP
                     LYERFLKIAEQEGLAINDRAPEDECICRAILAAFSDNLALRCNRGTLRCSLTHQRTGV
                     LARESVLRDQRMFTAAEITEVEGARREKNVLLSMATAVEEEWLEELFPGDCETRTEAR
                     FDTVLKRVVSTERRMFRDLVLSEKSSHMVDPEAAGKILAEEILAGRIRPGSWDGAVDE
                     WVVRVNCLARWCPDWELPAYDDEAWRCILQQ"
BASE COUNT          400 a          715 c          641 g          383 t
ORIGIN      
        1 ctgctgcaga atgcaccgcc atgcttcgtc atcgtaggcc gggagttccc agtcgggaca
       61 ccagcgggca agacagttca cgcggaccac ccattcatcc accgcgccgt cccatgagcc
      121 gggcctgatg cggccggcca gaatttcttc ggccagtatc tttcccgcgg cttcgggatc
      181 caccatgtga gaagattttt cactgagcac caggtcacga aacatgcgcc tctcagtgga
      241 caccacgcgt ttaaggaccg tatcgaaacg cgcctcggtc cgggtctcgc agtcgccggg
      301 gaacagctct tccagccatt cctcctccac ggcggtggcc atgctcagca gcacgttttt
      361 ttcccggcgg gcgccttcca cctccgtgat ctcggccgcg gtaaacatgc gctgatcgcg
      421 cagcacactc tcgcgggcaa gaactcccgt ccgctggtga gtcagactgc agcgaagtgt
      481 tcccctgtta cagcgcagcg ccagattgtc ggagaaggcc gcaagtatcg cccggcagat
      541 gcactcatcc tccggcgcgc gatcgttgat ggccaggccc tcctgctctg cgattttgag
      601 gaaccgctca tacagcggct ggacctgccg cgcggacacc ccgtgaatcc cgagcgcctg
      661 acacgctccg ggagaataat tcttcgtttc ggcatatttc cacgccttca tcagcaggaa
      721 aaagtccgaa gaaaattcgt ccccgaaaag ctcctcccgt ttctccgcga ccggtttgcc
      781 ggcattccgc aggaggatcg gcggaccctg ggtcagggct gcgatcagtg cggcctgccg
      841 aacgcatcca tagtcatttg cggtcagcag catccgcgaa tagcgggggt gcaccggaaa
      901 acggaccatg cgccggccca tcggctgcag cctcccgtcg cgcccgagcg cacccagatc
      961 atgcagcagc gtttcagcct gttccagcgc cttctctccc ggggaatcca gccaggggaa
     1021 cccccggaca tcgtcaatcc cgcaggattt cagcatcaaa accacttccg caagatcaac
     1081 ccgcaggatt tcaggagtct cctgcaacgg ccgctgcgcg tgctcctgcg cggtccacag
     1141 gcgcaggcat cgccccgggc cagttcggcc cgcccggccg gagcgctggt cggccgaggc
     1201 gcagctgatc ttttccacat aaagggtgtc aatgccccgg tgcgggtcat agcgcgccat
     1261 ccgcgccagt ccgctgtcta tcaccgccgt cactccggcg atggtaatgg atgtttccgc
     1321 cacattggtg gcgacaatga tgcgcgggcg tccggattcg ctgacggcct catcctgcgc
     1381 ccggggcgag agctgcccgt gaagcggaag cacagccagt cccctcgcgc ggggatgtga
     1441 ctgcagcatc tctattgttt ttcgtatctc gtaggctccc ggcatgaaga tgagcacatg
     1501 gccctcaagg ccgccgtccg cgagccggcc gaaggcttca gcagcggcat cccagacggc
     1561 cgtttttctg aaatcgacgg ctctggggag atattcgacg gcgaccgggt agacacggcc
     1621 ctccgattcc agcacccggc agggagccag atgttcctgc agccgcgccg aatccagtgt
     1681 ggcggacatc acaatgatcc gcaggtccgg ccgcgaggtc tgctgcaggc ggagcgcagc
     1741 ggaaagagaa atgtccccat acaggtgccg ctcatgaaat tcgtcaaaga tcaccgtgga
     1801 gaccgccgcg agttcgggat catcaatcca ctggcggaga agcacgcctt ctgtcacata
     1861 acggatgcgg gtccggggac cggtgtggtt ttcaaaacgg acctgatatc cgatttcgcc
     1921 gccgacatcg ccgccgcgtt cagaagccac ccggtgagcc agcaggcgcg cggcgatccg
     1981 ccggggctgg agcacgacaa tctggcctcc gggcccgtcc agaagcatct gcgggatctg
     2041 cgtggatttc ccggagccgg tcggtgcgcg gataatgatc cggcagccgg gaaccgcggc
     2101 cgcgcgcagc gccgcctgca gttcatagac gggatagtc
//
LOCUS       DAOQWR010000234         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_59283_length_2137_cov_4.732949, whole genome shotgun sequence.
ACCESSION   DAOQWR010000234 DAOQWR010000000
VERSION     DAOQWR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59283_length_2137_cov_4.732949"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..918
                     /locus_tag="PKK36_11900"
     CDS             121..918
                     /locus_tag="PKK36_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR95299.1"
                     /translation="MKTCLVTGAKGFIGSAVAAEAARLGYEVTAVDLDNYEQLRGSQA
                     DILINAAGNSRKFIDEQDPQRGFELSVTSIMRIMLDFKCGLCVHLSSGTVYPNEGDPS
                     RNAEGANLKPREMTRYGFHKWLAEQMARHYAPRTLIVRMGGFVGPGLRKNAVFDILTD
                     GKLFVHPDSEFQYMDTRDLARLVFELAGGAAGNETLFNLSSTGTVSVRRIAEWAGYKL
                     TRDPLGCPTVRAELNVEKAGRLLALPETGETVRRFIEEVQSGKVKLK"
     gene            915..1901
                     /locus_tag="PKK36_11905"
     CDS             915..1901
                     /locus_tag="PKK36_11905"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HNR95300.1"
                     /translation="MIISRTPFRISFFGGGTDFPGYFTQHGGSVISVTIDKYCYLSVH
                     HLTKFFKHNYRASYSRTESVMKPEEFEHPLIRESLLYAGIREGVEISHVADLPGQTGL
                     GSSSAFTVGLMNSLCAFIKKRITPEWLARAAIEIERNRVKDAGGWQDQYAAAYGGFNR
                     INFQTDREVTVQTVGIAPEALFELEASLMLFYLGSECSAENILREQISRTGENFDRLS
                     RMRQIVDHAEDILQNGKHLDEFGKLLHETWLQKKELSSGISNPVIDEAYDAARGAGAI
                     GGKLLGAGGRGFLCLYAPTAKHAAVREKLKDLKEVSFRFSEEGSQIIFNSDE"
     gene            1894..>2137
                     /locus_tag="PKK36_11910"
     CDS             1894..>2137
                     /locus_tag="PKK36_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95301.1"
                     /translation="MSENKPRIAFWFRYGAAEHSELFHALPGCIRELSRHCEVHYFGL
                     KTRKIVPMELRENAILHEFPFYVNRGSGLDKMLKTAL"
BASE COUNT          480 a          613 c          606 g          438 t
ORIGIN      
        1 tgacgtcgcc cgtctgcaca gtctcgggtt gtcctgcgat acaagcctcg aggacggcct
       61 ccgccgcaca accgaatggt tcatcaaggc ccgcaaagac gggacggtgc ggctgtaatc
      121 atgaaaacat gtctggtcac aggcgcgaag ggctttatcg ggtccgccgt tgcggcagaa
      181 gcggcgcgac tcggctacga agtgaccgcc gttgatctcg acaactacga acagctccgc
      241 ggttcacagg cggacattct gatcaatgcc gccggcaact cgcgcaagtt cattgacgaa
      301 caggatcctc agcgtggatt tgaactttcg gtcacttcca ttatgcggat catgctggac
      361 ttcaagtgcg gcctgtgtgt gcatctttcc agcggcacgg tttatccgaa cgaaggggat
      421 ccctcccgca atgccgaagg cgcaaatctc aagccccgtg aaatgacccg ctacggcttt
      481 cacaaatggc tggccgagca gatggcaagg cattatgccc ccagaaccct catcgttcgc
      541 atgggcggct tcgtggggcc cggcctcagg aaaaatgccg tcttcgacat tctgacggac
      601 gggaaacttt ttgttcatcc cgattcagag ttccagtaca tggacacgcg tgatctcgcg
      661 cggctcgtgt ttgaactggc cgggggagcc gcgggaaatg aaacactctt caacctttcc
      721 tccaccggca ccgtttccgt gcgcaggatt gccgagtggg ccggctacaa actgacgcgt
      781 gatccgctcg gatgtccaac cgtgcgcgcg gaactgaatg tcgaaaaagc cggccgtttg
      841 ctggcccttc cggaaacggg cgaaacggtc cgccggttta tagaggaagt ccaaagcgga
      901 aaggtgaagc tgaaatgatc atatcccgta caccgttcag gatcagtttt ttcggcgggg
      961 gtacggattt ccccggttac ttcacccagc acggaggatc cgtcatctct gtcaccattg
     1021 ataaatactg ctatctttcg gtgcatcatc tgacaaagtt tttcaagcac aactatcgtg
     1081 caagctactc aagaaccgaa tccgtcatga aaccggagga gttcgaacat cccctgatcc
     1141 gcgagtcgct tctttacgct ggaatccgtg aaggcgttga aatatcccat gtggcggacc
     1201 tgccgggcca gaccggcctg ggctcatctt ccgcattcac cgtgggcctg atgaactcgc
     1261 tgtgcgcctt catcaaaaag cgcatcaccc cggaatggct ggcgcgggcc gccatcgaaa
     1321 tcgaacgcaa ccgcgtcaag gacgccggag gctggcagga tcagtatgcg gccgcttatg
     1381 gcggcttcaa ccggatcaat ttccagacgg accgcgaagt gacggtccag accgtcggca
     1441 tcgcgcccga ggcccttttt gagctggaag cctcgctgat gcttttctat ttggggtctg
     1501 aatgctccgc ggaaaacatt ctgcgcgagc agatttcccg cacaggcgaa aacttcgacc
     1561 ggctgtcacg gatgcggcag atcgtggatc acgccgagga tattctgcag aacggcaaac
     1621 atctggacga gttcgggaag ctcctgcacg agacctggct ccagaaaaaa gaactctctt
     1681 ccggaatcag caatccggtc attgacgaag cctatgacgc ggcccgcgga gccggggcca
     1741 ttggcggaaa actgcttggc gcgggcgggc gcggtttcct ctgcctgtac gcgccgactg
     1801 caaaacacgc ggctgtccgg gagaaactga aggacctcaa ggaggtctcg ttccggttca
     1861 gcgaagaagg cagccagatc attttcaaca gcgatgagtg agaacaagcc cagaatcgct
     1921 ttctggttca ggtacggagc cgctgaacac agcgagctgt tccatgccct gcccggctgc
     1981 atccgtgaac tctcaagaca ctgtgaagtg cactatttcg ggctcaaaac ccggaagata
     2041 gtcccgatgg agctcaggga aaacgcgatt cttcacgaat ttccttttta tgtgaaccgg
     2101 ggaagcggcc tggacaaaat gctgaaaact gccttgt
//
LOCUS       DAOQWR010000235         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_59555_length_2132_cov_2.278767, whole genome shotgun sequence.
ACCESSION   DAOQWR010000235 DAOQWR010000000
VERSION     DAOQWR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59555_length_2132_cov_2.278767"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1064
                     /locus_tag="PKK36_11915"
     CDS             279..1064
                     /locus_tag="PKK36_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95302.1"
                     /translation="MQADQSRNEADAVQTMVARMIALSPVGQNLLLIGGFRYRLIDHS
                     SRISRDVDYHWTGNLVRKQEEMLSLLQKRVLPALKREFGYEGRVDPASGPEADSPSVR
                     SIVLSVWRPDPPGGRLEIPVEITKVSCADAPDIRTVDGVIYPTVSDADQIENKVISVF
                     NRLHLQHRDLVDIFLFSSKLLPDSPARLKEKLRALGVGPDVIDRKMKDFSEHSAYHAK
                     AIQAVIDSQLDPVAAANIAAAGGGGMILERVVQILQTMVCSKS"
     gene            1075..1665
                     /locus_tag="PKK36_11920"
     CDS             1075..1665
                     /locus_tag="PKK36_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNR95303.1"
                     /translation="MKALEWIRFLEEQRTRYGKVLFRVTELANAAGRDSHALNVELSR
                     LLKKGVLVRYANGLYGEPGAVNVEQLTVALDPGAYITGLYALHRHNLITQVPVEITCF
                     TNRAHSHSRKRKTPLGGMMFVHVSPQIYCRPRKGVIAGPEQAFCDFVYLARRRGVDPL
                     SLLTFRNLEKLRVRELSAMLKKYPTTVADAVREITG"
     gene            1842..>2132
                     /locus_tag="PKK36_11925"
     CDS             1842..>2132
                     /locus_tag="PKK36_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR95304.1"
                     /translation="MSLGRILVVEDDQQIADVVEHLLKSNDYEVRTAADGQAALRQFK
                     SYRPDLVFLDLNLPRLSGWEVLRQIRQERSDIPVIMLTSQTEEADRVLGLERG"
BASE COUNT          483 a          515 c          620 g          514 t
ORIGIN      
        1 cagcaggcag catattttga ctatctgcct gtttcttgtt ctttttccca atcttcatca
       61 ttgccttgcg gaccgccgcc caattcccag ctgtcatggt gtcgagcatg ttgatggcac
      121 gggaatggcc ctggatggcc tcagaaacca ttcgggatca aattattcct tcgccggcct
      181 gaaaaactct tcgaaaacag cttgacatac aggtattaat gattataaat atataacaat
      241 aatgttgtat attgattcat ataattaccg gagatcacat gcaggctgac cagagcagaa
      301 acgaagcgga tgctgtccag acaatggtgg cgagaatgat tgccttaagc ccggttggac
      361 aaaacctgct gctcatcggc ggctttcgct accggctgat tgatcacagc tccaggattt
      421 caagggatgt tgactatcac tggacgggga atcttgtccg aaagcaggag gagatgctgt
      481 cgctgctcca gaaaagagtg cttccagccc tgaaaagaga atttggatat gagggtcggg
      541 tggatccggc ttcgggaccc gaagccgatt cgccgtccgt gcgaagcatc gttctttccg
      601 tttggcggcc ggatccgccc ggcggccggc tggagattcc ggtggagatt acgaaggttt
      661 cctgcgcgga cgccccggat atacggaccg tggatggagt gatttatccc accgtttctg
      721 atgcggatca gatcgagaac aaggtgatat ccgttttcaa ccgccttcat cttcagcaca
      781 gggacctggt ggatatcttc ctgttcagca gcaagctgct gccggattcg cctgcccgtc
      841 tgaaagagaa gctgcgggca ctgggtgttg gcccggatgt catagaccgg aagatgaagg
      901 acttttcaga gcattccgct taccacgcga aagccataca ggcggttatt gattctcagc
      961 tggatccggt tgcggccgcc aacattgccg cagcgggcgg gggtgggatg attctggaaa
     1021 gggtggttca aattctgcag acaatggtgt gttcgaagtc ctgaagttga ttttatgaaa
     1081 gcgctggaat ggataagatt tcttgaggaa cagcggaccc gatatggcaa ggtcttgttt
     1141 cgggttaccg agcttgccaa tgcggccgga cgtgattccc atgcactgaa tgtcgaactg
     1201 agccgcctgt tgaagaaggg ggtgctggtt cgttatgcaa acggtctcta tggggagccg
     1261 ggtgccgtca atgttgagca gctgacagtg gcgctggatc cgggcgccta tattacgggt
     1321 ttgtatgcgc ttcaccggca caacctgata acgcaagttc cggttgaaat cacctgtttc
     1381 accaacaggg cgcacagcca ttcgcggaag agaaagacgc ccctgggcgg catgatgttt
     1441 gtgcatgtca gtccgcagat ctactgcagg ccgcggaagg gtgtaatcgc cggcccggag
     1501 caggcttttt gcgattttgt gtatctggcc cgccgccgcg gagtggatcc gctgagtctc
     1561 ctgacattca ggaatctgga gaagctgcgc gttcgggaac tttccgcgat gctgaaaaaa
     1621 tatcccacca ccgttgcgga tgctgttcga gaaatcactg gttgagattg tgccaacagg
     1681 gcagcatccc gccgttggtt ttccggccag aattccgtcc ggaaggaatg cgaaatattg
     1741 atcctgcggt gcttgatcca aaagacggat atccgtaaaa cctgggacaa atctgaaaat
     1801 atgctctcta ttcaaattca aatttggtat tcaggataat catgagtctg ggcagaatac
     1861 ttgtagtgga ggatgatcag cagattgccg atgtggtgga gcatctgctg aaatcgaacg
     1921 attatgaggt tcgtacggca gcggatggac aggccgcgct ccggcaattc aaatcgtacc
     1981 gccccgatct cgtctttctt gaccttaatc ttccccggct ttccggatgg gaggtgctgc
     2041 gccagatacg gcaggagcgt tctgatattc ccgtcataat gctgaccagt cagacggaag
     2101 aggccgaccg ggtgctgggg ctggagcggg gc
//
LOCUS       DAOQWR010000236         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_61461_length_2089_cov_4.265487, whole genome shotgun sequence.
ACCESSION   DAOQWR010000236 DAOQWR010000000
VERSION     DAOQWR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61461_length_2089_cov_4.265487"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKK36_11930"
     CDS             <1..909
                     /locus_tag="PKK36_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR95305.1"
                     /translation="GPAQKLAAQFGCEASDDWRRVVERDDIDIVAVCTPPHVHAEISI
                     AAMKAGKHVLCEKPLTRTVEEAEQMLAAAKDTGRTLKCGFNHRHHPAVWEAHQRVSRG
                     EIGRPLFARCRYGICGRPGYENEWRADPAKAAGGQFIEQGTHAIDLFRWFLGEITEAV
                     CMSGILYFKKQSMDDSGMALFRTDSGATASLHTTLTEWQNQFSFEVFGEDGYLKVEGL
                     GSSYGNERLIAGRRDFDAPFQDTVTHYRGGDISWREEWREFLAAIREKREPIGNGQDG
                     LMAMRIALTAYEAELQKRVLPVKGSF"
     gene            919..1410
                     /locus_tag="PKK36_11935"
     CDS             919..1410
                     /locus_tag="PKK36_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNR95306.1"
                     /translation="MKILVTGAGGLIGQCLSTRLREAGHKVHGLDVAGGEGILDADIM
                     DADRLKSLFAEIRPDGIFHLAAQSSPPRSWSEPAETFRMNVNGTLHVLEAVREVCPGA
                     TVVVACSSAEYAMSLDGRPIGEDYPLNPSNPYGISKVAAGELARVMDCAMICRCSAFA
                     PSF"
     gene            1383..1841
                     /locus_tag="PKK36_11940"
     CDS             1383..1841
                     /locus_tag="PKK36_11940"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR95307.1"
                     /translation="MLRVRPFFLIGPRKTGDVCSDFARGIVAVERGKQKELLTGNLEA
                     VRDVMDVRDGAAAFQAVMEKGEPGEVYNICTGCGVRIGELLEMFVKLAKVPVAHRADP
                     ARMRAIDEPVRIGDPSRLKALGWAPACALQDTVRDILDYWRKREDEPCRS"
     gene            1829..>2089
                     /locus_tag="PKK36_11945"
     CDS             1829..>2089
                     /locus_tag="PKK36_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR95308.1"
                     /translation="MQVVILAGGKGTRIQSIARDIPKALIPAAGKPFIEHQFDLLLRA
                     GIRDIVLCAGHLGDQIAGHVGDGSRWGLRVKYSFEDPRALMGT"
BASE COUNT          398 a          587 c          724 g          380 t
ORIGIN      
        1 ggtcccgcgc agaagctggc cgcgcagttc gggtgcgagg cgtccgatga ctggcgccgc
       61 gttgtcgagc gcgacgacat tgacatcgtg gccgtctgca cgccgccgca cgtgcacgcc
      121 gaaatcagca tcgcggcgat gaaggccggc aagcatgtcc tgtgcgaaaa accgctcaca
      181 cggaccgtgg aagaggccga gcagatgctg gccgcggcga aggataccgg ccggacactg
      241 aagtgcggtt tcaatcacag gcatcatccg gccgtctggg aggcgcatca gcgcgtcagc
      301 cggggcgaaa tcggccggcc gcttttcgcg cgctgccgct acgggatctg cggccgcccc
      361 ggttatgaga atgaatggcg ggcggacccg gcaaaagccg cgggcggaca gttcatcgag
      421 caggggactc atgccataga cctgttccgg tggtttctcg gcgaaataac cgaggctgtc
      481 tgcatgagcg gcattctgta tttcaagaag cagtccatgg atgacagcgg catggcactg
      541 tttcggacgg acagcggtgc cacggcaagc ctgcacacca cgctgaccga atggcagaac
      601 cagttttctt tcgaagtctt cggcgaggat ggatatctga aggtcgaagg cctgggcagc
      661 agctatggaa acgagcggct gattgccggg cggcgggatt ttgacgcgcc gtttcaggac
      721 accgtgaccc attatcgcgg cggcgatatt tcgtggcgcg aggaatggcg ggagtttctc
      781 gcggcgataa gggaaaagcg cgagcctatc ggcaacgggc aggacgggct catggccatg
      841 cggattgcgc tgaccgccta tgaagccgaa ctgcagaagc gcgttctccc ggtcaagggc
      901 tccttttagg gatcgtgcat gaaaatcctc gtgacaggag caggcgggct gatcggacaa
      961 tgcctgtcca cccggctgag agaggccggg cacaaggtcc acggcctgga tgtggcgggc
     1021 ggcgaaggga ttctcgacgc tgacatcatg gatgcggaca ggctgaaatc cctttttgcg
     1081 gaaatccggc cggacgggat tttccatctg gccgcccaga gctctccgcc gcgctcatgg
     1141 agtgaaccgg cggaaacatt ccggatgaac gtcaacggca cactgcatgt gctggaagcc
     1201 gtccgggagg tctgtccggg ggcgaccgtc gtggtggcct gttccagcgc cgaatatgcg
     1261 atgtcgctcg atggccggcc catcggcgag gattatccgt tgaatccgtc caatccctac
     1321 ggcatcagca aagtggccgc gggcgagctg gcgcgggtta tggactgcgc aatgatctgc
     1381 aggtgctccg cgttcgcccc ttctttctga tcggcccgcg gaagaccggt gatgtctgct
     1441 ccgattttgc gcgcggcatt gtcgccgttg agagagggaa gcaaaaggaa ctgctgacgg
     1501 gaaatctgga ggccgtgcgc gatgtcatgg atgtgcgcga cggcgcggcc gcttttcagg
     1561 cggtgatgga aaaaggcgag ccgggcgagg tgtacaacat ttgcaccgga tgcggcgtgc
     1621 gcatcggaga gcttctggag atgtttgtca agctggcaaa agttccggtc gcgcatcgcg
     1681 cggatcccgc gcggatgcgg gccattgacg aaccggtgcg gatcggcgat ccctcaagac
     1741 tgaaggcgct tggctgggct ccggcctgcg cactgcagga cacagtgcgg gatattctgg
     1801 attactggcg gaaacgggag gatgaaccat gcaggtcgtg attcttgcag gagggaaggg
     1861 gacccgcatt cagtccattg cccgggacat tcccaaggcc ctgattcccg cggcgggcaa
     1921 accgttcatc gagcaccagt ttgatctgct ccttcgcgcg ggcatccgcg acattgtgct
     1981 gtgcgccggg catctgggcg accagatcgc cggccatgtc ggcgacggct cccggtgggg
     2041 gctgcgggtg aaatattcct ttgaagaccc gcgggcgctg atgggcacc
//
LOCUS       DAOQWR010000237         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_65402_length_2011_cov_2.459611, whole genome shotgun sequence.
ACCESSION   DAOQWR010000237 DAOQWR010000000
VERSION     DAOQWR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65402_length_2011_cov_2.459611"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKK36_11950"
     CDS             <1..198
                     /locus_tag="PKK36_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR95309.1"
                     /translation="CLTLRLNTERPVTIREGTNRLLDIEANTAKVVLETYSSRYKAKP
                     ARIEGWEGHAAKRIVDALGCG"
     gene            195..1352
                     /gene="wecB"
                     /locus_tag="PKK36_11955"
     CDS             195..1352
                     /gene="wecB"
                     /locus_tag="PKK36_11955"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR95310.1"
                     /translation="MKKISVIFGTRPEAIKLAPVILQLRRTPGVITEVCVTAQHRQML
                     DQVLQVFKIKPDVDLNLMEKNQNLGQFTARALLALEGYFESHQPDLVLVQGDTTTVLS
                     ASLSAFYHHVPVGHVEAGLRTGNLQAPWPEEANRILTSQLARLHFAPTETSRQNLLRE
                     GIRDDHIFITGNPVIDALYLVLAQVRETKPIVPGLPSSLQPYEKDRPKTILITGHRRE
                     NFGAGFEEICRAIVVLAQNFPKVQFIYPVHLNPNVRDPVWRVLRPEMNPNIHLIEPLS
                     YLNFVAMMDNSILILTDSGGIQEEAPSLGKPLLVMRDVTERPEALASGCVRLVGPHAD
                     CIISEVSRLLTDSNAYGEMVQTYNPYGDGHAAEYIVKIAMNYLNNARCKDK"
     gene            1388..>2011
                     /locus_tag="PKK36_11960"
     CDS             1388..>2011
                     /locus_tag="PKK36_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR95311.1"
                     /translation="MTLHADACAYDRLKWLKHHLRKGPQNTLDAGCGIGLFTLYAAKR
                     GNNVLGLSFDRSQLDTAETCRDLLGLQNASFKICDLRELDKSDIKRGSYDQIICCEVI
                     EHILDDQKLIRDLSAFLKPGGMIFLTTPFKGNKGLLGDYISSSEDGGHVRWGYTHDEI
                     RELFKKVHLDIVDTSYVSGIISQKAANAWRVLRNINGPLSRLANMLYR"
BASE COUNT          527 a          448 c          511 g          525 t
ORIGIN      
        1 tgcctcactc tccgcctgaa caccgaacgg ccggtgacca tccgtgaggg caccaacagg
       61 ctgctggata ttgaagcgaa tactgctaaa gtagtcttgg agacatattc ttcgcgttat
      121 aaagcgaaac ccgccaggat tgagggctgg gaaggccacg ccgcaaagcg gattgtggat
      181 gcattggggt gtggatgaaa aagatttcag tcatattcgg aacaagaccg gaagcgatta
      241 agctggcgcc tgtcattcta cagttacgtc ggactccggg agtaattacc gaagtatgtg
      301 tgacagcaca gcatcggcaa atgctggatc aggtcctgca ggtattcaaa atcaagcccg
      361 atgtggattt gaatctaatg gagaagaatc aaaacctggg gcagtttact gcccgcgctc
      421 ttcttgcact cgagggctat tttgagagtc atcagccgga tttggtgttg gttcagggtg
      481 acacaaccac tgttttaagc gcttcattgt ctgcttttta ccatcatgtt cccgtcggac
      541 atgtggaagc aggtttgcgc acgggcaatc tgcaggcccc ctggccggaa gaagccaacc
      601 gcatattaac gtcgcaattg gctcgattgc atttcgcccc cacagaaaca agtcgccaga
      661 atcttttacg agagggtatt cgagatgatc acatcttcat aacgggcaat cctgttattg
      721 acgctctgta tctggtttta gcgcaagttc gcgaaacaaa gccgattgtg ccgggtttgc
      781 ccagttccct gcaaccatac gaaaaagacc gtcccaaaac cattttgatt acagggcatc
      841 gccgggaaaa ctttggggcg ggatttgagg aaatctgcag agccatagtt gtgctcgctc
      901 agaattttcc caaggttcag tttatttatc cggtgcattt gaatccgaac gtacgcgatc
      961 ccgtgtggcg cgttttgcgt ccagaaatga atccaaatat acatttgatt gaacccttat
     1021 cctatttgaa ttttgtagca atgatggata attcaatact gattttaaca gattcgggcg
     1081 ggattcagga agaagcgccc agtttgggca agcctttgct ggtaatgcgc gacgtaacgg
     1141 aacgcccgga agcgctggca tcaggttgtg tcaggcttgt ggggcctcac gcggactgca
     1201 ttatcagcga agtatccaga cttctcactg actcaaacgc ctatggagaa atggtccaaa
     1261 catataatcc ctatggtgac ggccacgcag cggaatatat tgtgaaaatt gccatgaact
     1321 atttaaacaa tgcaaggtgc aaagataaat gaatcatagg ttaacacgga tatttggttt
     1381 ttgggggatg actcttcatg cggatgcatg cgcatatgac agattgaaat ggctgaagca
     1441 tcatctccga aaagggcccc aaaatacttt ggatgccggt tgcggcatcg gcttgttcac
     1501 tttgtacgct gcgaagcgcg gaaacaatgt tcttggcctg tctttcgata ggtcacaact
     1561 tgacacggct gagacttgtc gtgatcttct cggtctgcag aatgcctctt tcaaaatatg
     1621 cgatctcaga gaacttgata aatcggatat taaacggggc tcatacgatc agatcatttg
     1681 ttgcgaggtc atcgaacata tattagatga ccagaagctg atacgagatc tctctgcatt
     1741 tcttaaaccc ggcggcatga tatttctgac aacaccgttc aaaggcaaca aagggttgtt
     1801 gggtgattac atttccagtt ccgaagatgg tggacatgtt cgttggggtt atacccacga
     1861 tgagattcgg gagcttttta agaaagttca cctggatatt gtcgacacat cttatgtcag
     1921 cggaattatt tcgcagaaag cggcaaatgc ctggcgagta ttgcgtaaca tcaatggccc
     1981 tttaagccgg ttggccaata tgctatatcg t
//
LOCUS       DAOQWR010000238         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_67073_length_1980_cov_4.657662, whole genome shotgun sequence.
ACCESSION   DAOQWR010000238 DAOQWR010000000
VERSION     DAOQWR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67073_length_1980_cov_4.657662"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1980
                     /locus_tag="PKK36_11965"
     CDS             <1..>1980
                     /locus_tag="PKK36_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95312.1"
                     /translation="NAILVEKRRSAGGSEIHNFVSSWAFASGQDRHSLSHVPLFADAD
                     SWDFHLKSAQGRYEPGTGYATNDAPGVYSPMLDTGDPSSVFTNETAPRGPRINMGLYG
                     NTGQSSLSRTNAWLLAITLNGAGAISGTQDIYWAYGNIDTSETVRLEYSLNGGIEYFT
                     LATNIPVSQESVSWDVSEYNNTARGLWKVVLESDPAVYDAVDNEFSIRNQTRIYYVND
                     TNTAGDVYVDAGALGSPTNTGLTADSPMDSPLTVLQNYSVLAGDWIYIDTGVYVLTND
                     LKLDILNRGQSGMAVRIIGSTNMQAGGTILDRSSTNENSAVIKLEETSYIELEHLRLT
                     GGGRGVLFDNSLDCSLSWVEAYGNIGNGFDVLAGYPYRFDHCLSRNNGGWGISVQSIQ
                     GEAFFNQGILWSNRLGSAEAQAGSLAISNSILHAAGSTNAVYGLDELGRFYNDFNVIW
                     PVGKALVGRDVKRKISFRRLQDWQNDRDQDLHSQWTFPQFADDENGDFHLKTDHPEGR
                     YSTTAGVWTSDAEVSWAIDAGAFSASFANEPDPNGSRLNAGLYGNTWQASKTSTNVQF
                     RAVTLNDGGSINGPLTLYWLVMGMATDETVRLDYSWDGGFNWTNIATGLDPYQDGYYW
                     DPSGYESSPSGLWRVSSETDTNRLDIVDTNFLMRIG"
BASE COUNT          442 a          505 c          594 g          439 t
ORIGIN      
        1 caacgcgatt cttgtggaga aaagacgatc tgccggcggc agcgagatcc ataattttgt
       61 ttcatcctgg gcttttgcct ctggacagga ccgccacagc ctgagccatg ttccgctctt
      121 tgcggatgca gacagctggg atttccatct gaagagcgcc cagggccgtt atgagccggg
      181 aacgggctat gcgaccaatg atgccccggg agtttattcg cccatgctcg ataccgggga
      241 tccctcatct gtcttcacca acgaaacggc accaaggggc ccgcggatca acatggggct
      301 gtacgggaac accgggcagt ccagcctgag ccggactaat gcgtggctgc tggcaattac
      361 ccttaacggg gccggcgcga tttccggcac acaggatatt tactgggcct atggcaatat
      421 tgataccagc gagacggtca ggctggaata ttcgctgaac ggaggaatcg aatacttcac
      481 tcttgccacc aacatccctg tatcgcagga gtctgtttca tgggatgtgt cagaatacaa
      541 caacacagcc cgcggcttgt ggaaggtggt gctcgaaagc gatccggcag tctatgatgc
      601 ggtggacaac gagttcagca tccggaatca gacccggatt tactatgtca acgataccaa
      661 tactgcgggg gatgtctatg ttgatgccgg cgcgctcgga agtccgacca ataccggcct
      721 gacggcggat tcccccatgg acagtccgct cacggtcctg cagaattatt ctgttctggc
      781 cggcgactgg atttatattg atactggcgt ttatgtgctt accaatgacc tcaagctgga
      841 catcctgaac cgcggtcaaa gcggcatggc ggtgcggatt atcgggagca cgaatatgca
      901 ggcgggcgga acgattctgg atcgcagttc gaccaacgaa aactcggcgg tgattaagct
      961 tgaagagacc agctatattg aactggaaca tctccggctg acgggtggcg gccgcggcgt
     1021 tttgtttgac aacagccttg attgttcttt gtcatgggtt gaggcatacg gaaatatagg
     1081 caatggattt gacgtgctgg cgggatatcc gtacaggttt gatcactgcc tgtcccgcaa
     1141 caacggtggc tggggcatca gcgtgcagag tattcagggc gaggctttct tcaatcaggg
     1201 aatattgtgg tccaacaggc tgggaagcgc cgaggcccag gccggcagcc tggccatcag
     1261 caactccatt ctgcacgcgg cggggtccac aaatgcggtg tatggtctgg atgaactggg
     1321 ccgtttctat aatgacttca atgttatttg gccggtgggg aaggcgcttg ttggcagaga
     1381 tgtgaagcgg aagatatcct tcaggcggct tcaggactgg cagaacgaca gggaccagga
     1441 tctccacagc cagtggacgt tcccgcagtt tgcggatgat gaaaacggcg atttccatct
     1501 gaaaacagat cacccggagg gccgatacag tactacagcg ggagtgtgga cttctgatgc
     1561 cgaagtgtcc tgggcgatag atgcgggagc tttttctgca tcattcgcca atgaaccgga
     1621 tcctaacggc tcgcgtctca atgcgggctt gtacggcaat acctggcagg ccagcaagac
     1681 ctcgaccaat gtgcagttca gggctgtgac cttgaatgac ggcggcagca tcaacgggcc
     1741 gttgactctc tactggctgg ttatgggaat ggccaccgat gaaacggtgc ggctggacta
     1801 ttcatgggat ggaggtttca actggaccaa tatcgccacc ggcctggatc cttatcagga
     1861 cggttactac tgggatcctt cgggttatga gtcgtcaccc agcgggcttt ggcgtgtaag
     1921 cagcgagacg gacaccaacc ggctggatat tgtcgacacc aacttcctca tgcgcattgg
//
LOCUS       DAOQWR010000239         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_67176_length_1978_cov_4.075923, whole genome shotgun sequence.
ACCESSION   DAOQWR010000239 DAOQWR010000000
VERSION     DAOQWR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67176_length_1978_cov_4.075923"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PKK36_11970"
     CDS             complement(<1..713)
                     /locus_tag="PKK36_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95313.1"
                     /translation="MNAANPLVTIVIVTRNRAHTLRRALRSCMAQSYLPLEILVYDDA
                     SVDQTPAVVRSEFPGVHLAVADRRVGLIVLRNRGFRDARGEIVFSLDDDAYFADSGTV
                     SRTVEDFAGNACVAAVAIPFMEIFGADAGAKPSDGGDMIPGQRVSSYRGCAYAIRRRV
                     ALAVGGYRDYLVHQGEERDLCIRLREAGYEIIMGSAPPIVHDFSPTRTPMRMRYYGIR
                     NTLLFDVLNIPAPYVWGRF"
     gene            complement(710..1498)
                     /locus_tag="PKK36_11975"
     CDS             complement(710..1498)
                     /locus_tag="PKK36_11975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR95314.1"
                     /translation="MTVTSRNWKTGNLKGDSIHTSEQSANIYSDGTYREKNPDWHSQD
                     ATGKAVDVMPFIIRILDDCARPVRIADIGAGMGGVSEELNKLLRQKRPDADVQFECFD
                     LSPSAVEEGRRLFPDLNFHCREVGDADGPWDVCLLIDVLEHLENPHAWLRKLRSKCRF
                     VILRQPLQENFSTWRHANYRAQRSQWGHVSFFNERSFRCMAEAAGWEMQDTGLFLAGE
                     YGQSRGTRIPLHRRLIMSLNRDWAAYIFGAYLIGTLKSRELKVP"
     gene            complement(1434..>1978)
                     /locus_tag="PKK36_11980"
     CDS             complement(1434..>1978)
                     /locus_tag="PKK36_11980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR95315.1"
                     /translation="IGMCLGISALSILTALHENANGGRLISIDPLQTDPAHEKGIGLL
                     NVKRAGLDGLHTLIEKKSYEALPALLADGLQVDFGYIDGWHSFDYALLDFFYLDKMLS
                     VPGIVGFNDCGWRCVHKVLKFVQRYRRYREMDVGIAPSYKASNHLFSLIKRIEGRISN
                     DRYFQKLEDWEPEGGFYTHF"
BASE COUNT          465 a          593 c          498 g          422 t
ORIGIN      
        1 atgaaacgcc cccagacata cggggctggg atattaagca catcaaacag cagtgtgttt
       61 cggatcccgt agtaccgcat tcgcattggt gtgcgcgtag ggctgaaatc atgcactatc
      121 ggcggcgccg agcccataat gatctcataa ccggcttctc tcagacggat gcagagatca
      181 cgctcttccc cctgatgaac cagatagtct cgatatcccc cgacggccag tgcgacccga
      241 cgccggatgg cataagcaca gccgcgatag ctggatacac gctggccggg gatcatatcg
      301 cctccatcag acggtttcgc accggcatcc gctccgaata tttccataaa gggaatcgcc
      361 acggcggcca cgcacgcgtt ccctgcaaaa tcttccaccg ttcgcgacac cgtgcccgaa
      421 tccgcgaaat acgcatcatc atcaagagaa aacacaattt cgccccgtgc atcccgaaaa
      481 ccccggttgc ggagaacgat gaggccgact cttctgtcag ccacggccag atgcactccg
      541 gggaactcgg aacggaccac agccggcgtt tggtctacag atgcgtcatc atacacaagg
      601 atttccagag gcaaataact ctgcgccatg caggatcgca gcgcgcggcg cagcgtgtgt
      661 gcacggttgc gcgtgacaat gacaattgta acaagagggt ttgccgcatt catggcactt
      721 tcagttccct gctcttcagt gtgccgatca gataggctcc gaatatatag gcggcccagt
      781 cacgattaag gctcatgatc agtctgcggt gcagcggaat tcttgtcccg cgcgactggc
      841 cgtattcgcc cgcgagaaag agcccggtgt cctgcatttc ccatccggca gcttccgcca
      901 tacagcggaa acttcgttca ttgaagaacg atacatgccc ccactgactg cgctgggccc
      961 ggtagttggc atgacgccag gttgaaaagt tctcctgcaa cggctgacga agaatcacaa
     1021 agcggcattt tgagcgcagc ttcctcagcc atgcatgagg attttccagg tgctcaagaa
     1081 catcaatcag cagacataca tcccaggggc catctgcatc gccgacttcc cgacaatgga
     1141 aattcaaatc cggaaacagc cgccggccct cttccactgc agacggtgag agatcaaagc
     1201 attcaaactg aacatctgca tccggccgct tctgccgcag gagtttgttg agttcctcgc
     1261 tgacaccgcc cattcccgcc ccgatgtctg caattcggac aggccgggcg cagtcatcca
     1321 gaatccggat aatgaacggc atcacatcca ccgccttgcc ggttgcatcc tgactgtgcc
     1381 agtcaggatt cttttcgcga taggtcccgt cagagtatat attcgcgctc tgttcagaag
     1441 tgtgtataga atcccccttc aggttcccag tcttccagtt tctggaagta acggtcattg
     1501 ctgatccggc cttcgatacg cttaataagc gaaaacaggt gattggaggc tttgtagctt
     1561 ggggcgattc ccacatccat ctccctgtac cggcgatagc gctgcacaaa cttcagcacc
     1621 ttgtggacgc agcgccagcc gcagtcattg aatcccacta tgccgggaac gctcagcatt
     1681 ttatcgagat agaaaaaatc aagcagcgca taatcgaaac tgtgccaccc gtcaatatat
     1741 ccgaaatcaa cctgcagccc gtcggcgaga agtgccggaa gggcctcgta agactttttt
     1801 tcaataagcg tatgcagtcc gtccagccct gcgcgtttca cattcagcag gcctatgccc
     1861 ttttcatggg ccgggtccgt ctgaagcgga tcaatagata tcagccgccc tccgtttgcg
     1921 ttttcatgca gcgcagtcag aatgctgagc gcagagatgc ccaggcacat gccgatct
//
LOCUS       DAOQWR010000240         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_67916_length_1965_cov_2.852356, whole genome shotgun sequence.
ACCESSION   DAOQWR010000240 DAOQWR010000000
VERSION     DAOQWR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67916_length_1965_cov_2.852356"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1593
                     /locus_tag="PKK36_11985"
     CDS             217..1593
                     /locus_tag="PKK36_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNR95316.1"
                     /translation="MKSRPFIIVALNIAVAVGAPAADILEIGAEGDGIALQWEFPGVL
                     ESAAELSGPWIERPQAVASLVLPAAAPSFFRVRKPVYSVVDPGQTNCFSPSGIITPPL
                     SGEDFYGQDAQFDGPASSYTNNGDGTVTDLETGLMWVRARGFKMTWSNALSGAETNRT
                     GGYSDWRMPTIKELYSLIRFNGVNGPDNMTTAGYVPFIDTNYFEFAYGSGTGGERVID
                     CQDWSATEYVSTTMNNDETIFGVNFADGRIKGYPKYIPGGGGEGYVLYVRYVRGNPAY
                     GVNSFTNNGDGTITDMATALMWSQADSGTGLDWPSALAWVQAQNASNHLGYSDWRLPN
                     IKELQSIVDYTRSPSTTATAAIDTNYFACSPITNEAGQSDYPYYWSATTLLDGAPSPT
                     GAYISFGRAMGYAPLLAAWVDVHGAGSQKSDPMTGDPADFPAGRGPQGDAVRIYNYVR
                     LVRGGLKQ"
BASE COUNT          441 a          548 c          557 g          419 t
ORIGIN      
        1 tcatgccgcc tcctctcgat atccgtgtgc atttgaaccg caccattata ccggccctgc
       61 ccgcttcgcc aaccctattt ccggccggcg cgggaaattc cacaggcaat aaatctccgg
      121 ggaccccgtt atgcgaaaca ggagatatgc tggtatttcg aggtgagcat gagaacgatc
      181 aaagagacag ccggaaaccg gacaggagta aaccggatga aatcccgccc attcatcatt
      241 gttgccctga acattgcggt ggccgttggt gcgccggcgg ccgatattct ggagataggc
      301 gcggagggtg acggcattgc actccagtgg gaatttccgg gtgtgctgga gagtgccgcg
      361 gaactttccg gcccatggat agaacggcct caggccgtgg cctcgctggt tctcccggcc
      421 gctgctccgt cgtttttccg cgtccgcaaa cccgtctatt cggttgtgga tcccggacag
      481 accaactgtt tcagtccttc cggaataatc accccccctc tgtccggcga ggatttctac
      541 ggacaggatg cccagtttga cggaccggcc tcatcataca cgaacaatgg tgacggaacc
      601 gttaccgatc ttgaaacggg tctgatgtgg gttcgggcgc gcggtttcaa aatgacctgg
      661 agcaatgccc tgagcggagc ggaaaccaac cgcaccggcg gatatagcga ctggagaatg
      721 cccacgatca aggaactgta ttcgctgatt cgattcaacg gcgtgaacgg tccggacaac
      781 atgacaacag caggctatgt gcccttcatt gacaccaact attttgagtt tgcctatgga
      841 agcgggacgg gcggtgaacg ggtgattgac tgtcaggact ggtcggcaac ggaatatgtc
      901 agcaccacca tgaacaatga tgaaaccatc ttcggagtta atttcgccga cggccgcatc
      961 aagggttatc ccaaatacat ccccggcgga ggcggagagg gatatgtgct ctatgtgcgc
     1021 tatgtaaggg gtaatccggc ttatggtgtc aattccttca caaacaacgg cgacgggacc
     1081 ataaccgaca tggcgacggc gctgatgtgg tcgcaggcgg acagcgggac cggtcttgac
     1141 tggccttccg cgctggcctg ggtgcaggcg cagaacgcgt caaaccatct cggatacagc
     1201 gactggcggc tcccgaacat caaggagctt cagagcattg tggactacac ccggtcaccg
     1261 tccaccacgg caaccgcggc gattgacacg aattattttg catgcagccc catcacaaat
     1321 gaagccggac agagcgacta tccatattac tggtcggcga caacacttct ggacggcgcc
     1381 ccctcgccga cgggagccta tatttccttc ggccgtgcga tgggatatgc ccccctgctt
     1441 gcggcatggg ttgatgtcca cggtgcaggt tcgcagaagt ccgatcccat gaccggcgat
     1501 cccgccgact ttcccgccgg ccgcggtccg cagggcgacg ctgtgcgcat ttacaactat
     1561 gtccgccttg ttcgcggtgg cctgaaacag taggatatgc tgtgctgttc agtggatacc
     1621 gcagagggca tctggacgtt cgagccggcc tgcgttgggc ggagaactct gctttaccag
     1681 ctccctgccg caggccgccg gattttatcg gcttggggtc tgtgtggagc cttgactaag
     1741 acacgggccg aaaaattgta ataaattatt ctaaaagaaa atattactga aattcgaaac
     1801 catatagtca taaatgtata aaaaatatac atatgaccac atggtcactg ttctatggat
     1861 tgagtacccg gtagaatctc ggagaagctg cgggacccgg ccgtgagatc atgaacgccc
     1921 ccgcccccgt ttcctgaacc gtcgctgcgg tttcatttga ataag
//
LOCUS       DAOQWR010000241         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_69527_length_1938_cov_2.794477, whole genome shotgun sequence.
ACCESSION   DAOQWR010000241 DAOQWR010000000
VERSION     DAOQWR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69527_length_1938_cov_2.794477"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKK36_11990"
     CDS             complement(<1..514)
                     /locus_tag="PKK36_11990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95317.1"
                     /translation="MKVLHIVYDLMRGGTEGQCARVAMACRKLGHDHRVAVFARSGYF
                     LPQVEEACGPVYEVQIRRMLSLQTLREVLRLRDFIRAEGFDLVHTWDMDAGIFGSLAA
                     HLADVPYITSRRNLAEVMPAYKKRLLKIADRGAGAVVVNAKAVANHFIARGLDASKVC
                     MIPNLLDLEEF"
     gene            complement(675..>1938)
                     /locus_tag="PKK36_11995"
     CDS             complement(675..>1938)
                     /locus_tag="PKK36_11995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR95318.1"
                     /translation="FHFHNHFINDRLDESTLRLRASAGESLCLLIVKTEKQHFDRGRP
                     LRVLFVNHMASMIRGGGETFDLEIAKALNRMNCECTLLTSRPIWCGPHNRPDCIHTVY
                     IRSPYTRCIPEAFIPGRWRLRVLDCRIFEHLAAKWAYSHRTDFDIVQACEQVYFVEEW
                     THRQTGIPVVMRLTSPDYYDPVGAVQKAAAVIASGTTMGKMKAGERPDCVDIPNAVDP
                     RYFYPRPSEFRRNHGIGSADPLFIYAARMVPFKRHQWLLDVFASLLPELPAAKLLLVG
                     HGQLEDELKKYCAQKGLLDSVIFMGQMPYEKMPEIYAAADIKVIASDHRESFCFAALE
                     AMAMELPLVSTACGMLPSLIGENEGGRIVDIDDAEGFRSALLSLAADSSARRVIGARN
                     REYVIAHHRWDASARKLRTLYERLVALG"
BASE COUNT          480 a          535 c          495 g          428 t
ORIGIN      
        1 caaattcttc gaggtccagc aggttgggga tcatgcatac tttcgatgca tccaggcccc
       61 gcgcaatgaa atggttggcc accgcctttg cattgacgac cacggccccg gcgccgcggt
      121 ctgcaatttt cagcagccgt ttcttgtagg caggcatgac ttcggccaga ttgcggcggc
      181 tggtgatata gggaacatca gcaagatgtg ccgcgagaga cccgaatatc cccgcatcca
      241 tgtcccaggt gtggacaaga tcaaatcctt cggcccggat gaagtctctg agccggagga
      301 cttcccgcag cgtctgcagg gagagcatcc tgcggatttg cacctcatat accggcccgc
      361 aggcctcttc gacctgcggc agaaaatatc ccgatcttgc aaatacagcg acacggtggt
      421 catgtccgag tttccggcat gccatggcca cgcgggcgca ctgcccctcc gtgccgccgc
      481 gcatcaggtc gtagacaatg tgcaggactt tcatgcagga taaaggttga aggttgaagg
      541 taaaaggtta caggaaagaa tggagatcag aaaccggaga ctttaaatgc ggttgcagtt
      601 ggaatattgg gagaatggaa tgatgatgtt gtggaagaaa cccattattc cagtattcca
      661 cctcaccacc ctcttcatcc gagggcgacc agccgctcat aaagtgtccg cagcttccgg
      721 gcgcttgcgt cccatctgtg gtgcgcaatc acatattcac gattgcgcgc gcctattacc
      781 cgccgggcgc tggagtcagc cgcgagagac aacagggcgc tgcgaaaacc ttccgcatca
      841 tcaatgtcaa caatccggcc accctcattt tctccaatca gcgaggggag cattccacac
      901 gccgtgctga ccagcggaag ctccatggcc atggcttcca gcgcggcaaa gcagaacgac
      961 tcccgatggt cactggcaat caccttgata tccgccgccg catagatttc gggcattttt
     1021 tcatagggca tctgtcccat gaatataaca ctgtccagta agcctttctg cgcgcaatat
     1081 tttttaagct catcttcgag ctgaccatgg cccaccagaa gcagtttcgc cgccggcaat
     1141 tccggcagga gacttgcaaa aacgtccaga agccattgat gtctcttgaa agggaccatc
     1201 cgggcggcat atatgaatag cggatcggcg gagccaatgc cgtgattgcg ccggaattcc
     1261 gaaggtcgag gataaaaata acggggatcc acggcattgg gaatgtcgac acagtccggt
     1321 cgctctcctg ctttcatttt tcccatagtg gtgccactcg caatcacggc ggccgctttc
     1381 tgaactgcgc ccaccgggtc ataataatca ggagaagtca accgcatgac aacaggaatc
     1441 cctgtttgcc tatgcgtcca ttcttcaaca aaatacacct gctcacaagc ctgaacgata
     1501 tcaaagtctg tccgatgtga gtatgcccat ttggccgcca gatgttcgaa tatcctgcag
     1561 tccagcacac gcaaacgcca tcttcccgga ataaacgctt cgggaataca tcgcgtgtac
     1621 ggagatctga tataaacagt atggatgcaa tccggcctat tatgcggccc gcaccaaatg
     1681 gggcggcttg tcagcaatgt acattcgcag ttcatacgat tcagtgcttt tgcaatttca
     1741 agatcgaagg tttctccacc accacgaatc atacttgcca tatgattcac aaacagaacg
     1801 cgcagggggc ggcctctgtc aaaatgctgt ttttcagttt tcacaattaa aagacaaaga
     1861 gattctcccg cagaggcgcg gagacgcaga gttgattcat ccagccgatc attgataaag
     1921 tggttgtgaa aatggaag
//
LOCUS       DAOQWR010000242         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_69968_length_1931_cov_2.066098, whole genome shotgun sequence.
ACCESSION   DAOQWR010000242 DAOQWR010000000
VERSION     DAOQWR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69968_length_1931_cov_2.066098"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKK36_12000"
     CDS             complement(<1..211)
                     /locus_tag="PKK36_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95319.1"
                     /translation="MLSRRAQAAFGTIRPISDIGPEVAGALQVLLWHGMALKGIGLQG
                     LSNATYFPEIEFTIATSPFTCGIQQR"
     gene            complement(509..985)
                     /gene="tagD"
                     /locus_tag="PKK36_12005"
     CDS             complement(509..985)
                     /gene="tagD"
                     /locus_tag="PKK36_12005"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HNR95320.1"
                     /translation="MKKIITYGTFDLLHIGHVNLLRRARELGDHLTVAVSTDEFNEGK
                     GKKTVLPFPERMEIVKSIRYVDEVIPETSWEQKEGDIRNYQINVFTMGDDWAGKFDFL
                     KPLCEVVYLERTKHISSTQLKAELSRLQAVHGVLDKISRNDLKDMITILDRIVNLK"
     gene            complement(982..>1931)
                     /locus_tag="PKK36_12010"
     CDS             complement(982..>1931)
                     /locus_tag="PKK36_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR95321.1"
                     /translation="IYLNGAILGMKRTEIAGKFDEIVEFSGISRFIDTPVKRYSSGMY
                     VRLAFAVAAHLDPEILLIDEVLAVGDAEFQKKCLGKMKDVAGQGRTVLFVSHNMPAVL
                     NLCSRCVLLKQGAIEQIGETEAVVRRYLDSGQTQGAADIGARKDRGGNGLVRLTGFKV
                     RREGAEADLDELSLHDNAIFELSVHVGASTEISVRVRIRDEFGKTISYFSMLPRGLAL
                     HVDTGEQRFLLKLPGIPLIEGAYYIDVLIFDRYSITLLDDIENVRRLIMLPKDVHGAG
                     YRIGSGYGVSYIPHFWNTAADAPGTELIEFAKQGQANIP"
BASE COUNT          475 a          563 c          434 g          459 t
ORIGIN      
        1 ctctttgctg gattccgcag gtgaacgggc tcgttgcgat tgtgaactcg atttccggga
       61 aataagttgc attggacaaa ccctgcaatc caattccctt caaggccatc ccatgccaca
      121 acagaacctg aagtgcccct gcaacttccg gcccaatgtc tgaaatggga cggatcgttc
      181 caaatgcggc ctgcgcgcga cggctcagca ctctcgccat ccagcttttt ctcgggaccc
      241 atggcaaacc ggacttcagc atcaggtaat aagatcgtct gggggccacg ccaatcacca
      301 ctgcacccgg aagacggcat gtgtttttca gaaagtgaaa tgcattccct gcaaatattg
      361 gagaatggca ccccacaaga aaaagaggtt tccttgaaaa aaatgcccgc acaaaagtcc
      421 gaacactcac aagggaatat cggaagttca gatatgcggc atacagccgc tctgcaagcg
      481 tagaaggggt ccacttcatt gaagccgtct atttaaggtt cacgattctg tccagaatcg
      541 tgatcatatc cttcagatcg tttcgactga ttttgtcaag caccccatga accgcctgca
      601 gacgtgaaag ttccgccttc aactgtgtac tggatatgtg ctttgtgcgc tcaagataca
      661 ccacttcaca cagaggtttc aggaaatcga atttcccggc ccagtcatcc cccattgtga
      721 acacattgat ctgataattg cggatgtcac cttccttctg ctcccagctg gtctcgggaa
      781 tgacttcatc cacataacga atacttttaa ctatctccat gcgctccggg aacggaagca
      841 cggtcttctt gcccttgccc tcgttgaatt catcagtgga aaccgccacg gtcaaatgat
      901 cgccaagttc ccgggcacgg cgcagaagat taacgtgccc gatatgcagc aaatcaaatg
      961 tgccgtatgt tatgatcttc ttcatggaat gtttgcctgc ccctgtttgg caaattcgat
     1021 caattcagtt cccggcgcat cggcggctgt attccagaaa tggggaatat aggaaacacc
     1081 atagcccgaa ccaattctgt atcccgcccc atgaacatct ttcggcagca tgatcagtct
     1141 ccgaacattt tcaatgtcgt ccagcagggt tatggaatag cggtcaaata tgagaacgtc
     1201 tatataataa gcgccttcga tcagcggaat gccgggcaac ttcagaagga accgctgctc
     1261 ccctgtatcc acatgcaggg ccagtcctcg agggagcatg gaaaagtagc ttatcgtttt
     1321 gccgaactca tcgcgtatcc gcacacgaac cgatatttca gtggacgcgc cgacgtgcac
     1381 actgagttca aaaatcgcgt tgtcatgcag ggacaattca tccaaatcag cctctgcacc
     1441 ctcgcgtctc accttgaacc ccgtcagccg cacgagcccg ttcccccccc ggtctttgcg
     1501 tgcgccgata tccgctgcgc cctgcgtctg gccgctgtcg agatagcgcc gcacaacggc
     1561 ttcggtttca ccgatctgct caattgctcc ctgtttcagc aatacacatc tgctgcacag
     1621 attcaatact gccggcatgt tatgactcac aaaaaggact gttctgccct gcccggcgac
     1681 atccttcatc ttgcccaggc attttttctg gaactccgca tcgccaacgg caagaacctc
     1741 gtcaatcagc agaatctcgg gatcaagatg cgcggccacc gcaaacgcca gccggacata
     1801 cattccgctg gaataccgct tcaccggggt gtctataaaa cggctgattc cggagaattc
     1861 tacgatttca tcgaatttcc cggcgatctc ggtcctcttc attcccagaa tagctccgtt
     1921 caaataaatg t
//
LOCUS       DAOQWR010000243         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_70039_length_1929_cov_4.596585, whole genome shotgun sequence.
ACCESSION   DAOQWR010000243 DAOQWR010000000
VERSION     DAOQWR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70039_length_1929_cov_4.596585"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKK36_12015"
     CDS             <1..699
                     /locus_tag="PKK36_12015"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNR95322.1"
                     /translation="AEAYRVLRTNMQFAAKKSGGAYVVVSGGVGEGKSTTLFNLAFIC
                     AQLGDKVLIVDTDLRRPVQHTILGMSNRFGLTNVLMRDVPIEETIKATSLPNLHFLPS
                     GKLPRSSLGLLDSQRLRELVKNLKSRYDYVFFDSPPVMGVSDASILVSEMDAVLLVIQ
                     YRKYPKMISTRAKQIVENVGGKLIGVVLNNINIMRDDYYYYYHSYYSHYYAPGEESQV
                     EPPPASKAEQEGRI"
     gene            705..1712
                     /locus_tag="PKK36_12020"
     CDS             705..1712
                     /locus_tag="PKK36_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNR95323.1"
                     /translation="MKTGMRMALFGAVMLSMLTGCRSNRPPAYSYPDYSSRYTRQARE
                     YEQAREKARREEEISRQKEEKARLEREKKAEKERKKAEEKRLAAAQRAARAQTPSQPA
                     AAERSAPPAASPIRPAVHPAATPSEIAPAPARLEVTAPVARRPAPSAARPAVAVPPPS
                     VPREPVVVTESRSSSGSSPYQLKAGDMVAIYLRGIPREEEIQDVVDENGYVTLTYINE
                     VHAAGKTTSELERAIREAYLKAEIYKNISVNVVMQQQSYFVRGEVRSAGRFVLLSGTT
                     LMQAIATAGGYTEFAQPKKVRLLRGDNSTLHDLKQIEKNPEKDISIEAEDVIVVPRSV
                     F"
     gene            1722..>1929
                     /locus_tag="PKK36_12025"
     CDS             1722..>1929
                     /locus_tag="PKK36_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95324.1"
                     /translation="MQPTDEIREPRRRRRRSHVVPVPFSVRRRMYDGIILTLLLAPSL
                     AGLFLYGGVGTWSISLLMIFSSAGL"
BASE COUNT          387 a          570 c          579 g          393 t
ORIGIN      
        1 gccgaggcct atcgcgtgct gcggaccaac atgcagttcg cggccaagaa gtccggcggc
       61 gcctatgtgg tggtgagcgg cggtgtgggc gaaggcaaat ccacgaccct gttcaatctg
      121 gccttcatct gcgcccagct gggggacaaa gtgctgattg tggacacgga tctccgccgt
      181 cccgtgcagc acaccattct gggcatgtcc aaccgtttcg gactcaccaa cgtgctgatg
      241 cgcgatgtgc cgatcgaaga aaccatcaag gccaccagtc tgccgaatct gcatttcctt
      301 cccagcggaa agcttccccg ctcctcgctg ggcctgctgg acagccagcg gctgcgcgaa
      361 ctggtcaaaa atctcaagag ccgctatgac tacgtcttct tcgattcgcc gcctgtgatg
      421 ggtgtgagcg atgcctcgat tctggtgagt gaaatggacg cggttctgct ggtgatccag
      481 tacaggaagt accccaagat gatttccacc cgcgccaagc agattgtcga aaatgtcggc
      541 gggaagctga tcggggtggt gctcaataat attaacatca tgagggatga ttactattat
      601 tactatcatt cctattattc tcactactac gctcccggcg aggagtcgca ggtggagccg
      661 ccgccggctt caaaagcgga acaggagggg agaatttgat cagcgtgaag acaggaatgc
      721 gaatggcgct cttcggggca gtgatgttgt ctatgttgac cggctgccgg tcgaaccgtc
      781 ctccggcata cagctatcct gattattcct cccgctatac gcggcaggcg cgtgaatacg
      841 agcaggcccg cgagaaggcc cggcgcgagg aggagatcag ccgccagaag gaagaaaagg
      901 cccgcttgga gagggagaag aaggccgaga aggagcggaa aaaagcagaa gagaaacgcc
      961 ttgccgctgc gcagcgcgcc gcgcgggcgc agaccccgtc ccagccggcc gccgctgaac
     1021 ggtccgctcc gcccgctgct tctccgattc gcccggccgt tcatcctgct gcgactcctt
     1081 ccgaaatcgc gccggcgccg gcgcgtctgg aggtgaccgc tcccgttgcc cgccgccctg
     1141 cgccgtccgc ggcccgtccg gctgttgcgg ttccgccccc gtcagttccc cgggaacccg
     1201 ttgtcgtgac ggaaagccgt tcctcatccg gaagctcccc gtatcagctc aaggcgggcg
     1261 acatggtggc catctacctg cggggcattc cccgcgagga ggagattcag gatgttgtgg
     1321 acgaaaacgg ctatgttacg ctgacctaca tcaacgaggt tcatgcagcc ggaaagacca
     1381 catctgaact ggagcgcgcc atccgcgagg cctatctgaa agctgaaatt tacaaaaata
     1441 tcagcgtcaa tgtggtcatg cagcagcaga gttactttgt gcgcggtgaa gtgagaagcg
     1501 ccggccgctt tgtgctcctc agcggcacca ccctgatgca ggccattgct acggcgggcg
     1561 gctacaccga atttgcccag ccgaagaagg tgcggctgct gcgaggcgac aattccacgc
     1621 tccatgacct caagcagata gagaagaatc ccgagaagga catttccatc gaggcggagg
     1681 atgtgattgt ggttccccga agcgtatttt agtctgggcc aatgcagccg accgatgaaa
     1741 tccgggaacc ccgccgccgt cgccgccgaa gccatgttgt tcctgttcct ttttctgttc
     1801 gtcgcagaat gtatgacggg atcattctga ctcttctgct ggcgccgtcg cttgcgggcc
     1861 ttttcctcta tggcggggtg gggacctggt ctatttcgct cctgatgatc ttttcttctg
     1921 ccggtctgc
//
LOCUS       DAOQWR010000244         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_75685_length_1841_cov_3.223964, whole genome shotgun sequence.
ACCESSION   DAOQWR010000244 DAOQWR010000000
VERSION     DAOQWR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75685_length_1841_cov_3.223964"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PKK36_12030"
     CDS             <1..619
                     /locus_tag="PKK36_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR95325.1"
                     /translation="LRAINGFADALVEEAGDDIPDTCRHFLAVITRNARSMGQLIDDL
                     LSLSRLGRKPLERLRVDMNTLVREVAAECRPDPAVRQVEFQVEPLPPAQADLVLIRQV
                     WTNLISNAVKFTKNTEHAKIHIWSFEENGQTVYAIRDNGAGFNMDYVDQIFGVFQRLH
                     RAEDFEGTGVGLAIVHRIIQRHGGTIRAEGRENKGAAFFFTISED"
     gene            complement(623..>1841)
                     /gene="gatA"
                     /locus_tag="PKK36_12035"
     CDS             complement(623..>1841)
                     /gene="gatA"
                     /locus_tag="PKK36_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNR95326.1"
                     /translation="LNVKGQPCTCASKILKGYTAPYTATALARLEEQGAVLLGRTNMD
                     EFAMGSSTENSAYKVTRNPWNTDYVPGGSSGGSAAAVAAHEAVSAIGTDTGGSIRQPA
                     AFCGCVGLKPTYGRVSRYGLTAYASSLDQIGPITKDVRDAAILLQAMAGHDPLDSTSV
                     DLPVPDYATALQSGLKGMKLGMPKEYFIEGIDPEVEKAVRSAAEKCRELGAEIVDVNL
                     PHTPYAIAVYYIIATAEASANLARFDGVRYGARVEGDSPIDMYGKTRAAGFGEEVKRR
                     IILGTYVLSSGYYDAYYARAQKVRTLIRKDFEQVFETCDAVLAPVAPTAAYKIGEKTS
                     DPLQMYLGDIFTVTANLAGICGMSVPCGRTASGLPIGLQILGPAFKEENILRAGYAYE
                     QASGFSLCGPPAR"
BASE COUNT          362 a          561 c          567 g          351 t
ORIGIN      
        1 gctccgcgcc atcaacggct ttgcagacgc gctcgttgaa gaggccggag acgacatccc
       61 cgatacctgc cgccatttcc tggccgtgat cacccgcaac gcccgcagca tgggccagct
      121 gattgacgac ctgctttcgc tttcccgcct gggccgaaaa ccgctggagc gcctgcgggt
      181 tgacatgaac acgctggtca gggaggttgc tgctgaatgc cgtcccgatc cggccgtgcg
      241 ccaagttgaa tttcaggttg aacccctgcc cccggcgcag gccgatctcg tgctgatccg
      301 ccaggtgtgg accaacctga tttcaaacgc cgttaaattc acaaaaaata ctgaacatgc
      361 gaagatccat atttggtcgt tcgaggaaaa cgggcagacc gtctatgcca tccgcgacaa
      421 cggagcgggg ttcaacatgg attatgttga ccagatattc ggcgtgtttc agcgcctgca
      481 ccgcgcggag gactttgagg ggacgggcgt gggcctggcc atcgttcacc gcatcatcca
      541 gcggcatgga ggaaccatcc gcgccgaggg ccgtgaaaac aaaggcgcgg cgttcttctt
      601 cacaatctcc gaggactgag gctcaccggg ccggcgggcc gcacagggaa aatccggatg
      661 cctgctcata cgcataaccg gcgcgaagga tgttctcctc cttgaaggcc ggacccagta
      721 tctgcaaacc aatcggcagg ccggacgcgg tccgtccgca gggaaccgac atcccgcaga
      781 tgcccgccag attggcggtg acggtgaata tgtcgcccag atacatctgg agtggatcag
      841 aagttttttc cccgattttg taggcggctg tcggggcaac aggagccaga acggcatcgc
      901 aggtctcaaa cacctgctcg aagtcctttc ggatcagggt gcgcaccttc tgcgcgcggg
      961 cgtaataggc gtcataatag ccgctcgaaa gcacataggt gcccaggatg atgcggcgct
     1021 tcacttcctc gccaaagccg gccgcgcggg tcttgccgta catatcaatc gggctgtcgc
     1081 cctccacgcg cgccccgtag cgcactccgt caaaccgcgc aagattggca gaggcctcgg
     1141 cggtggcaat gatgtaatag acagcaattg cgtatggcgt gtgcgggaga ttgacgtcaa
     1201 caatttcggc ccccagctcc ctgcatttct ccgcggcgga gcgcacggcc ttttccactt
     1261 cgggatcaat gccctcgata aaatattcct tcggcattcc gagcttcatg cccttcaggc
     1321 cggactgcag ggctgttgca taatccggca ccgggaggtc cactgaagtg gaatccagcg
     1381 gatcgtgccc ggccatggcc tgcagaagaa tggcggcatc acgaacgtcc ttggtgatgg
     1441 gcccgatctg gtcgagcgac gaggcatagg ctgtcagtcc gtaccgggaa acacggccgt
     1501 aggttggctt cagcccgacg cagccgcaga atgccgcggg ctgacggatc gaaccgccag
     1561 tgtctgttcc aatggccgaa acggcttcat gggcggcgac ggcggcggcg gagccgccgc
     1621 tggagccgcc ggggacatag tcggtgttcc acggattgcg ggtcaccttg taggcggagt
     1681 tctccgtcga ggagcccatc gcaaattcgt ccatgttggt gcggccaagc agaacggcgc
     1741 cctgctcttc cagccgcgcc agggccgtcg ccgtgtaggg cgcggtatag cctttcagaa
     1801 ttttcgaggc gcaggtgcag ggctgtcctt tcacgttcag c
//
LOCUS       DAOQWR010000245         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_77551_length_1814_cov_3.147811, whole genome shotgun sequence.
ACCESSION   DAOQWR010000245 DAOQWR010000000
VERSION     DAOQWR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77551_length_1814_cov_3.147811"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PKK36_12040"
     CDS             <1..603
                     /locus_tag="PKK36_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNR95327.1"
                     /translation="GAAKMQRDSFREYLNLYLKALLHLDPERAARKGALDGYISYSGM
                     RGQVRWALEHYSRARLITSLRPFDSYAVSHIKSRYQNADMTPELLQEAWEHWYHKVID
                     YLYLKSVFPDRVCLVSFDDLVANTEAAARAICRFLDISYDPCMLVPTLFGCPVKGNSA
                     RPQDDKMRGRIYSDRRRLEKNRIPEDGHNLWKAFEAIKSI"
     gene            626..1309
                     /gene="pseF"
                     /locus_tag="PKK36_12045"
     CDS             626..1309
                     /gene="pseF"
                     /locus_tag="PKK36_12045"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNR95328.1"
                     /translation="MMGLITARGGSRGLPGKNIRALCGKPLIAHTIEAAVQSGCFESV
                     LASTDSQEIADVCLKYGAEVPFLRPAHLAADDAKSRDVVLHALEFMGQRAPVDSVCLL
                     QPTSPLRSAQDIRRAVSIFRQNSADSVISVTEYSHPIQWAVSLSEDGRISETSVLKPS
                     RRQDLSVCYHPNGAIYIFNAEFIRRSESYIGKNTYSLIMPPERSVDIDTPFDFLLAEF
                     IYTHIREEK"
     gene            1306..>1814
                     /locus_tag="PKK36_12050"
     CDS             1306..>1814
                     /locus_tag="PKK36_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNR95329.1"
                     /translation="MIKECTISRRDTLLETLKALSASGEGIALVVDENNRLEGVATDG
                     DIRRALLKGAKLTDAIEPFMTKDYRFVSPDRSRADVLDLMQAQQLRHIPIVSAGKELM
                     GVHLLRKMIARDKKPNWAVVMAGGKGTRLYPITEKIPKPMLEVAGRPILERIVLHLVG
                     HGIQQIFLSV"
BASE COUNT          406 a          475 c          540 g          393 t
ORIGIN      
        1 ggggcggcga aaatgcagcg ggattccttt cgggaatatc tgaacctcta tttgaaggcg
       61 ctgctccatc tggatccgga acgcgcagcc cgcaaaggtg cgctggatgg atatatcagc
      121 tattccggaa tgcgaggcca ggtccgctgg gcgctggagc actacagccg ggcgcgactg
      181 atcacttctc tgcggccgtt tgacagttat gcggtctctc acatcaagtc gagatatcag
      241 aatgccgaca tgacgccgga gctgctgcag gaggcctggg agcactggta tcacaaggtc
      301 atcgattatc tctatttaaa atcggtattc ccagaccggg tgtgcctggt gtcgtttgat
      361 gatctggttg cgaacacgga agcagctgct cgggccattt gccgattcct tgacatcagt
      421 tatgacccat gcatgctggt gccgactctg tttggctgtc cggtgaaggg gaattcagcc
      481 cgcccgcagg atgacaagat gaggggcagg atatattcag accggaggcg gctggagaag
      541 aaccgcattc ccgaagacgg ccataatctg tggaaagcgt ttgaagcgat caaaagcatt
      601 taacgggcaa aaaggcggaa gcactatgat gggactaata acagcccgcg gaggctcgcg
      661 aggccttccg ggcaagaaca tcagagccct ctgcggtaaa ccgctgattg cccacacgat
      721 tgaagcggcg gtgcagagcg gctgcttcga atctgtcctg gcgtcaacag acagtcagga
      781 gattgcggat gtctgcctga aatacggcgc ggaggttccc ttcctccgtc cggcgcattt
      841 ggccgcggat gatgccaaat ccagggatgt ggttcttcat gctctggagt ttatgggtca
      901 gagggcgccg gtggacagtg tctgcctgct tcagcccaca tcaccgctgc gttccgcgca
      961 ggatatccgg cgagccgtat ccatattcag acagaactcg gctgattctg tgatcagcgt
     1021 gaccgaatac agtcatccga tccagtgggc cgtttccttg tccgaagacg ggaggatcag
     1081 cgagacttcg gttctgaaac cttcccggcg gcaggacctg tcagtttgtt atcatccgaa
     1141 cggcgcaata tacatattca atgccgaatt catccggcgc tctgaaagct acatcgggaa
     1201 gaacacgtac tccctcatca tgccgcccga gcggtcggtt gacattgaca ccccttttga
     1261 ttttctactg gcggaattta tatataccca tataagggaa gaaaaatgat caaggaatgc
     1321 acaatcagca ggcgggacac ccttcttgaa accctgaagg ccctgagcgc gtccggggag
     1381 gggattgccc ttgtcgtgga tgagaacaac cggctggagg gtgttgcaac cgatggagat
     1441 atccgccggg ccctgctcaa gggggcgaag ctgaccgatg ccattgagcc cttcatgacc
     1501 aaagattacc gttttgtgtc gcccgaccgc agccgtgcgg acgttctgga tctgatgcag
     1561 gcccagcagc ttaggcacat tccgattgtg agcgccggca aggagctgat gggtgtgcat
     1621 ctgttgagaa agatgatagc ccgcgacaaa aagcccaact gggcggttgt gatggcgggc
     1681 ggcaagggca cgcgccttta tcctatcacg gaaaagattc ccaaacccat gctcgaagtg
     1741 gctggccggc cgatcctgga gcgcattgtt ctccatctgg tgggtcacgg gattcagcag
     1801 atatttcttt cggt
//
LOCUS       DAOQWR010000246         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_82069_length_1752_cov_5.784325, whole genome shotgun sequence.
ACCESSION   DAOQWR010000246 DAOQWR010000000
VERSION     DAOQWR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82069_length_1752_cov_5.784325"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1752
                     /gene="mfd"
                     /locus_tag="PKK36_12055"
     CDS             <1..>1752
                     /gene="mfd"
                     /locus_tag="PKK36_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNR95330.1"
                     /translation="SDILGYQKVERRGIAKTGRKKPPAISRPILSQTEIQPGEYVVHV
                     GCGIGKYLGLYEIRTSGQLQEVLAVEYADGAKLYVPVAQSHLLSRYMGVGRRRPQLHR
                     LGGGRWDRQKEAAEQAIMDMSASLLETQAAREMLRGHAFPPDDKWMHEFEASFPFEET
                     PDQARAITEVKQDMESHKPMDRLVCGDVGYGKTEVAMRAAFKAVASGKQVAILVPTTI
                     LAQQHYDTFSQRMAAYPFKIEMLSRFRTKAEQDRVIEMLGEGQIDIVIGTHRLVQRDV
                     GFKDLGLVIIDEEQRFGVGHKEHLKHLRRLVDVMTLTATPIPRTLYMSLTGAKDMSTI
                     QSPPQERLPIETIVTETEDHTIRQAILRELNREGQVFFLHNRVKSIGIIAEKLMKIVP
                     EARIAVAHGQMAERELAAVMRKFAAREMDVLLCTTIIESGLDMPNVNTILIDRADRFG
                     LADLYQLRGRVGRYKHRAFAYFLLPKDRLLYNTAQERIRAIQRYSSLGAGFKVALRDL
                     EIRGSGNILGRQQSGHIAAIGFDLYCQLLRQTIAKSRGERLSPAVRCELKLDFITLSA
                     QQAEAAGCAAIPYDYME"
BASE COUNT          365 a          539 c          566 g          282 t
ORIGIN      
        1 gagcgacatt ctcggctacc agaaggttga acggcgcgga atcgcaaaaa ccgggcgcaa
       61 aaaacctccg gccatcagcc gcccgatcct atctcagacc gaaatccagc cgggcgaata
      121 tgtggtgcac gtcggctgcg gaatcggaaa gtatctgggg ctctacgaaa tcaggacaag
      181 cggccagctt caggaggttc tggccgtgga gtatgccgac ggcgcgaaac tctatgtgcc
      241 ggtggcccag agccacctgc tgagccgcta catgggcgtg gggcgcaggc gcccgcagct
      301 tcacaggctg ggcggcggcc ggtgggaccg gcagaaggag gccgccgagc aggccatcat
      361 ggatatgtcc gcttcgctgc tcgaaacgca ggccgcccgc gagatgctca gggggcacgc
      421 gttcccgcct gacgacaagt ggatgcatga gttcgaagcc tcattcccgt tcgaggaaac
      481 gcccgaccag gcgagggcga tcaccgaggt gaaacaggac atggaaagcc acaagccgat
      541 ggaccggctg gtctgcggcg atgtgggata cggaaaaacc gaggtcgcca tgcgcgcggc
      601 gttcaaagcg gtcgccagcg gcaagcaggt ggccatcctt gtgcccacca caattcttgc
      661 gcagcagcac tacgacactt tttcgcagcg aatggcggcc tatccgttca agattgaaat
      721 gctgagccgc ttccgcacga aagccgagca ggaccgcgtc attgagatgc tgggcgaggg
      781 gcagattgac atagtgatcg gcacgcaccg gctggtgcag agggacgtgg ggttcaagga
      841 tctggggctc gtgatcatag acgaggagca gcgcttcggc gtggggcaca aagagcattt
      901 gaagcatctc cgccggctgg tggacgtcat gacgctgacc gccaccccga tcccgcgcac
      961 gctttacatg agtctgaccg gggcgaagga catgagcacc atccagtcgc cgccgcagga
     1021 gcggctgccc atcgaaacca tcgtgaccga gacggaagac cacaccatcc gccaggccat
     1081 cctgcgcgaa ctgaaccgcg agggacaggt gttcttcctc cacaaccgcg tgaagagcat
     1141 cggcatcatc gcggagaagc tcatgaaaat tgttccggag gcgcgcatcg cggtggcgca
     1201 cgggcagatg gccgagcgcg agctcgcggc cgtgatgagg aaattcgccg cccgcgaaat
     1261 ggacgtgctg ctgtgcacca cgatcatcga aagcgggctg gacatgccga acgtcaacac
     1321 gatcctcatt gaccgcgccg accgcttcgg gctggcggac ctctaccagc tgcgcggccg
     1381 cgtcggccgc tacaagcacc gcgccttcgc ctattttctg cttccgaagg accggctgct
     1441 ttataacacc gcgcaggagc gcatccgcgc gatccagcgc tattccagcc tcggcgccgg
     1501 gttcaaggtc gcgctgcggg atctcgaaat ccgcgggtcg ggcaacattc tgggccgcca
     1561 gcagagcgga cacattgcgg ccatcggctt cgatctgtac tgccagcttc tccggcagac
     1621 cattgcgaaa agcagggggg agcggctttc gcctgcggtc cgctgtgaac tcaagcttga
     1681 tttcatcacg ctgtccgccc agcaggcgga ggccgccggc tgcgcggcca ttccctatga
     1741 ctatatggag ga
//
LOCUS       DAOQWR010000247         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_84832_length_1717_cov_3.515644, whole genome shotgun sequence.
ACCESSION   DAOQWR010000247 DAOQWR010000000
VERSION     DAOQWR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84832_length_1717_cov_3.515644"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1717)
                     /locus_tag="PKK36_12060"
     CDS             complement(<1..>1717)
                     /locus_tag="PKK36_12060"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR95331.1"
                     /translation="PNPADPDTDGDGMLDGWEMVNGLNPVDPGDDGGDLDGDGLSNLE
                     EFQNNTDPNAADSDGDGLTDGDEVNNRLTDPTLADTDLDGLDDGYEVGIGTEPTDADS
                     NGNYLSDGDMYQLGWDPSVPMDVYNVWLEETFETSTWNNWVHHAPNAYMPIDLWHLSQ
                     AEPATDLSNITYMDNRSTNTAYRFADDVHLGTNTAADYNKGMALRAALESWQFDPLTA
                     SNLYIQWTEYYDTEPASDYMEVLARGSENPNWLVVSTAVSGRSGVPQDGSEPARWVRR
                     RADLSRFTGSSNVQVRFLFTAENPVNNDYPGWYVDDVLIYEGTTIRGWVRNENGAPLA
                     GAVVQAIGRGGVNEYIDGHRYINPGKIFAEVKTAEDGSYVMSGLPQGRYYVKASFPNY
                     KSEFFDGPLVAGAYGFGSLDNPGVYDISSVEPSRGWIDLTAAGSSEECDFELEKGSSR
                     SYLGVMMAGQTQDVWVNGLTNALKARIWDGVASTNKPADDFDDYQTWAAGTPTNNHPD
                     WLNNPVKPNLLGDLAPGDHILYIGDGLGMVSAPAVTLRQGEVTLVDIQTNQPLGHVYV
                     STAGGS"
BASE COUNT          326 a          531 c          482 g          378 t
ORIGIN      
        1 cgctgcctcc ggccgtgctc acatacacat ggcccagggg ctgattggtc tggatatcca
       61 ccagggtcac ttccccctgc ctcagcgtca cggcaggagc ggagaccatt ccaagcccat
      121 ctcctatata aaggatatga tcaccgggag ccagatcacc cagcagattg ggcttcaccg
      181 gattgttcag ccagtccgga tggttgttgg tcggtgttcc cgcggcccag gtctgatagt
      241 catcgaaatc atccgccggt ttgttcgtgg atgcgacccc gtcccaaatg cgcgccttga
      301 gggcgttggt gagtccgtta acccagacat cctgcgtctg tccggccatc atgaccccga
      361 ggtagcttcg gctgctgcct ttttcaagtt cgaaatcgca ttcttccgaa ctgccggcgg
      421 ccgtcaaatc aatccatccg cgcgagggct caaccgaact gatgtcatac acacccgggt
      481 tgtcgagact gccaaagccg tatgcgccag cgaccagcgg gccgtcgaag aattccgact
      541 tatagttggg gaacgaggcc ttgacgtagt aacggccctg ggggaggccg gacatcacat
      601 agctcccgtc ttccgctgtc tttacttccg caaatatttt tccggggttg atatagcgat
      661 ggccgtctat gtattcattg accccgccgc ggccgatcgc ctgaaccacg gcgccggcca
      721 ggggcgcgcc gttttcattc cgcacccagc cgcgtatggt ggttccttca tagatcagca
      781 catcgtccac ataccagccg ggatagtcgt tgttgaccgg attctcggcc gtaaacagga
      841 aacggacctg aacattgctg gagcccgtga aacggctgag atccgcccgg cggcgcaccc
      901 agcgggcggg ttcggacccg tcctgcggga caccgctgcg gccggaaacc gccgtgctga
      961 ccacgagcca gttcggattt tcgcttccgc gggcaagcac ttccatgtaa tcgctggcag
     1021 gctcggtgtc gtaatattcc gtccactgga tataaagatt gctggccgtc agaggatcaa
     1081 actgccagct ctccagcgcg gcgcgaagcg ccatgccctt gttgtagtca gcggctgtat
     1141 ttgtgcccag atgcacgtca tcggcaaacc ggtaggcggt gttcgtcgaa cggttgtcca
     1201 tgtaggtgat gttcgaaagg tccgtggcag gctccgcctg cgaaagatgc cacaggtcaa
     1261 tcggcatgta ggcgttgggc gcatgatgca cccagttgtt ccaggttgaa gtctcaaagg
     1321 tttcttccag ccagacattg tacacatcca tgggaacgga gggatcccat ccgagctgat
     1381 acatatcccc gtcgctcagg taattcccgt tgctgtccgc atccgtgggc tctgtgccaa
     1441 tgcccacctc atagccgtcg tccagcccgt ccagatcggt atcagccagt gtgggatcgg
     1501 tcaaacggtt gttgacctca tcaccgtccg tcaaaccgtc cccatcactg tccgcggcgt
     1561 tcggatccgt gttgttctga aattcctcaa ggttggacag gccgtcgcca tccagatcac
     1621 cgccgtcgtc gccgggatct accggattca acccgttaac catttcccat ccgtccagca
     1681 tgccgtcgcc gtcggtgtcg ggatcggccg gattagg
//
LOCUS       DAOQWR010000248         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_86985_length_1690_cov_5.381040, whole genome shotgun sequence.
ACCESSION   DAOQWR010000248 DAOQWR010000000
VERSION     DAOQWR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86985_length_1690_cov_5.381040"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..>1690
                     /locus_tag="PKK36_12065"
     CDS             283..>1690
                     /locus_tag="PKK36_12065"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNR95332.1"
                     /translation="MSHHIKILILLTCAANAFAGTEDFANFPETGSQYLDGGFIGRDG
                     STWSYIQCRGDKPITTPTPGMASGKYPAANVASGTLTGGCGTISFDYMLMYGSNVNLD
                     VVINETIRHTISWTNGNLRNFITNSSLITINQGGNFTLKVAQHDSRAQQVAIDNIVWT
                     PYGGSAPEPPEILFSPVSTNIVIAYSNLITLTVTATEPNEDPARLWASSLPAGSTFNT
                     VTGLTPLNSTFSWRPDSAQTGNYSVIFYAGDKDGTNSRSFDIEVTPIYPYYHYAEGLT
                     GTNLKAKLHEIISTGAHELSNLEEEYAMKEIHTDPANTGAVILIYRKIPMAKTLYGAD
                     GWNKEHCWPVSYKLGDGPAEVDIHNLYAADTEVNRLRGNLYFDESDASDSGYTAPAAV
                     NATNCSYDSNSWEPPRDSKGNVARAVFYMAVRYDGNEPDTQDLEISDLPSTTNTMGIL
                     TTLLDEGGLDPFLEDIRTL"
BASE COUNT          434 a          507 c          402 g          347 t
ORIGIN      
        1 ccggccagat gcggatagac atcgctgatc ctgcgcccga catcctgcac actgatcagc
       61 gtcacaatcc gtgtttccgg atgactgagc agcagctcaa taattcccac gccgccgtac
      121 ccgctggccc ctaccacttt caccttgatc atcgttcacc gtcctgtttg ttcaccggac
      181 gcatttcact cccaacggac cgcaaagtca agccggagcc ttttgcatgc aggcggcagt
      241 tttctgtgga attcatttaa gaaacgggta tgtttatccg ccatgtcgca tcatattaaa
      301 attctgatcc tgctgacctg cgcggcgaac gcattcgccg ggaccgagga ttttgcaaac
      361 ttccctgaaa caggcagtca atatttggat ggggggttca tcgggcgtga cggatcaaca
      421 tggagctata ttcaatgccg cggggacaaa ccaataacca cacctacccc cggcatggct
      481 tccggaaagt atcctgcggc aaacgtggcc tcagggactc tcactggcgg atgcgggaca
      541 atctctttcg actacatgct gatgtacgga tcaaacgtca atctcgatgt cgtgatcaat
      601 gaaaccatac gccacaccat atcgtggacc aatgggaatc tgcgaaactt catcaccaac
      661 agcagtctaa taacaattaa tcagggcgga aatttcacgc ttaaagtggc acagcacgac
      721 agccgtgcac aacaggtggc cattgacaac attgtgtgga ccccctatgg cgggagcgct
      781 cccgaacccc cggaaattct gttctcgccc gtcagcacca atatcgtcat tgcatacagt
      841 aatcttataa ccctcacggt cacggccaca gagcccaatg aagaccccgc ccgcctttgg
      901 gcaagcagcc tgccagcagg gtcaacattc aacactgtga ccgggctgac gcccctcaac
      961 tccaccttct cctggagacc agactccgcg cagaccggaa actattccgt cattttctat
     1021 gccggcgaca aagacggtac caattcacgt tcattcgaca tcgaggtcac gcccatctat
     1081 ccctattacc attatgccga aggcctgacc gggaccaacc tcaaggcgaa gcttcacgaa
     1141 atcatctcca ccggcgcaca tgagctcagc aacctggaag aagaatacgc catgaaggaa
     1201 atacataccg atcccgcgaa caccggtgct gtgattctga tttatcgcaa gattccaatg
     1261 gcgaaaacac tttacggcgc ggatggatgg aataaggagc actgctggcc cgtttcatac
     1321 aagctcggtg acgggcctgc agaggtggat attcacaacc tctatgcggc ggacacagaa
     1381 gtgaacagac tgagaggcaa tctgtatttc gatgaaagcg acgcttcaga ttcaggttac
     1441 acagcccccg cagccgtcaa tgcgaccaac tgcagctacg acagcaactc atgggaaccg
     1501 ccgagggact ccaagggcaa tgtggctcgc gcagtgtttt acatggctgt gcgctatgac
     1561 ggaaacgaac ctgacacgca agacctcgag atatccgatc tgcccagcac cacgaacacc
     1621 atgggcatcc tgaccaccct gctggatgaa ggtggtcttg accccttctt agaggacatc
     1681 cgaaccctct
//
LOCUS       DAOQWR010000249         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_93343_length_1619_cov_9.584399, whole genome shotgun sequence.
ACCESSION   DAOQWR010000249 DAOQWR010000000
VERSION     DAOQWR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93343_length_1619_cov_9.584399"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1435
                     /locus_tag="PKK36_12070"
     CDS             95..1435
                     /locus_tag="PKK36_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HNR95333.1"
                     /translation="MKEDYTIQLEFAPLKGKKITARFDDPRVSSDGGAMLLREVDQQI
                     GLLDRLAAVISDNRRQSHIAHEVTDLLRQRAYQISCGYEDADDCDHLRADPVLKAAVG
                     RDPQVDPDLGSQPTMTRLENSVTARDLLRMGYAFIDQFIASYDAPPTVIVLDMDPTAD
                     IVHGHQQLALFNAYEDEYCFMPFHVYEGQSGKYIASVLRAGKTPTAAEIISVLKRIVK
                     RIRQVWPEVKIIFRADGHHARPEAMDWMDAHRVDFIIGLTPNNRLKTMFALAIQDAEK
                     HRSFGDTCRRYASVDYAAESWSRSRRVIGRVQASGEGTDVRYVVTSFRDAGAQYLYDV
                     VFCGRGRMELMIKDHKVALKSDRTSCTRKEANQFRLFIHSAAYVLMHAMRENLLRGSE
                     LATAQFDTIRLRLLKIGTRVEVGKTFIRFHFPASYPLQPLLGRISTILKALSTA"
BASE COUNT          373 a          437 c          455 g          354 t
ORIGIN      
        1 cctgaattat tcatcaagat tcaggaaaaa atgagtgagc tttctctacc cattctgata
       61 gaatgggttg tgtaaaaaga agaaagactc actcatgaaa gaagattaca cgattcagtt
      121 ggaatttgca cccttaaaag gcaagaaaat cacagcccgc tttgatgatc cccgagtcag
      181 ctctgatggc ggagcgatgc ttttgcggga agtggatcag cagattgggc ttttggatcg
      241 gctggccgca gtgattagcg acaaccgcag acagagccat atagcgcacg aggtcaccga
      301 tctgctgcgc cagagagcct atcagatcag ttgcggctac gaagatgcgg acgactgcga
      361 tcatctgaga gcggaccccg tgttgaaagc ggcggtgggg cgcgatccgc aggtcgatcc
      421 cgacctgggc tcacagccga ccatgacgcg gctggagaac tcggtaacgg cccgggatct
      481 tttgcggatg ggatatgcgt tcattgatca gttcatcgct tcttatgacg cacctccgac
      541 ggtcatcgtt ctggacatgg atccgaccgc ggacattgtt cacggtcatc aacagctggc
      601 gcttttcaat gcgtatgaag acgaatactg tttcatgccc tttcatgtgt atgagggcca
      661 gtccggcaaa tatatagcca gcgtgctccg ggccgggaag actcccacgg cggcagagat
      721 catcagtgtg ctcaagcgca tcgtgaagcg catccggcag gtctggccgg aggtgaaaat
      781 cattttccgc gcggatggtc atcatgccag accggaggcg atggactgga tggatgcgca
      841 ccgggtggac ttcattatag gcttaactcc caataacagg ctcaaaacga tgtttgcgct
      901 ggcgatacag gatgcggaaa agcaccgctc ttttggagac acctgccgac gttacgcatc
      961 cgttgattat gcggccgaat cttggagccg atcccgtcgg gtgattggcc gcgtacaggc
     1021 ctccggagaa ggaaccgatg tgcgttatgt ggtcacctcc ttccgggatg cgggagccca
     1081 atatctttat gatgtcgtct tctgcggccg cggaagaatg gagctgatga tcaaagacca
     1141 caaagtggca ctgaagtcag accggaccag ctgcacaagg aaagaggcca accagttccg
     1201 gctgttcatt cactcggccg cctatgtgct gatgcatgcg atgcgtgaaa accttctgcg
     1261 cggttctgaa ctggccaccg cccagttcga caccattcgt ctgcgtctgt tgaagatcgg
     1321 gacgcgcgtt gaggtgggca agaccttcat ccgtttccac ttcccagcca gttatccact
     1381 tcagccgctg ctcgggcgaa tctcaactat cctcaaggcg ttgtccacgg cataggactt
     1441 catctgcggc gggcctccgc gtcctgaaaa tacggaggag atggcggagc tttgccccga
     1501 tcctgtcttc accgcgatca atcgcccctg tctggagcct tcagcccttt cgaagtctcg
     1561 cgccccataa tccagacccc agatcgcgcc ccaaaagcaa agttgatgaa tagatcagg
//
LOCUS       DAOQWR010000250         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_108751_length_1477_cov_6.758790, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000250 DAOQWR010000000
VERSION     DAOQWR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108751_length_1477_cov_6.758790"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKK36_12075"
     CDS             complement(<1..345)
                     /locus_tag="PKK36_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR95334.1"
                     /translation="MNNVLVVSASWLGDAVMSMPALQAWQQANPEARISVLAKRGPAE
                     LWRMHSAPAEVLTLGTGPGDTWEAVNEVRQRKFTEAFILPHSFRSAVVPFLAGVPVRR
                     GMPGHFRDWMLTS"
     gene            complement(362..>1477)
                     /gene="lpxK"
                     /locus_tag="PKK36_12080"
     CDS             complement(362..>1477)
                     /gene="lpxK"
                     /locus_tag="PKK36_12080"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNR95335.1"
                     /translation="IEGRRKGRGAALLRGVLKALSCVFALLVQIRLRLYKHRILRHHT
                     LGCQVVSVGNLTVGGTGKTPIVEIFARSLQQQGRKVAILSRGYKKSKPPMGKRIIHKL
                     KRLERQTPPLVVSDGKRLLLESDMSGDEPYMLASNLPDVVVLVDKNRVKAGQYAIKHF
                     GCDTLILDDGFQYLALKHRLDIVLVDATNPFGNRSMLPRGLLREPVRNIRRASFIFIT
                     KCSPGGESELKTRLRELNPDAEISECRHCARYLENIYTGERMPLESLRNKAVAAVSGI
                     AAPKGFEDELVRLGARLIYHKRYADHHRYTEQEIIDLINKSLLREADMIITTEKDAVR
                     FPKIKRRDLPVFFLRVEIEMLSGMEDFNACIKRICFL"
BASE COUNT          298 a          450 c          414 g          315 t
ORIGIN      
        1 gctcgtgagc atccagtcgc gaaaatggcc cggcattccg cggcgaacag gaacgccggc
       61 cagaaaggga accaccgcag aacggaaaga atgggggagg atgaaagcct ccgtgaattt
      121 tctttgccgc acttcattga cggcttccca cgtatctccc gggccggttc cgagtgtcag
      181 gacttcggca ggggccgaat gcatccgcca gagttcggcg ggcccgcgtt tggccagcac
      241 actgatccgg gcttccggat tggcctgctg ccacgcctgc agggcgggca tgctcatgac
      301 ggcatcgccc agccagcttg cacttacgac cagcacatta ttcacggcat ttcccgtttg
      361 ctcataaaaa acaaatccgc ttgatgcagg cgttgaagtc ttccattcca ctcagcattt
      421 ctatttccac gcgcaggaaa aatacaggca gatcacgccg cttgattttc gggaagcgca
      481 ccgcgtcctt ctcggtcgtg atgatcatat ccgcctcccg cagcaggctc ttgttgatca
      541 gatcaatgat ttcctgttcc gtatagcgat gatggtcggc atagcgcttg tgatagatca
      601 gcctggcccc cagccgaacc agctcgtcct caaagccctt gggcgcggcg attccggaaa
      661 ccgcggccac ggctttgttc ctgagcgact ccagcggcat gcgctcaccg gtatagatgt
      721 tttccagata gcgcgcgcaa tgccggcatt ccgagatttc cgcgtctgga ttcaactcgc
      781 gcaggcgtgt tttcagctcg ctctcgccgc ctggactgca tttggtgatg aaaatgaaac
      841 tcgcgcgccg gatgttgcgc accggctcac gaagcaggcc ccgcggaagc atgctgcggt
      901 tgccaaaggg gttggtggca tccacaagaa cgatgtccag tctgtgcttc agcgccagat
      961 actgaaagcc gtcatccaga atcagcgtgt cgcacccgaa atgcttgatg gcgtactgtc
     1021 cggccttcac ccggttttta tccaccagaa caacgacatc aggcagattg gaggccagca
     1081 tgtagggttc gtccccgctc atatctgatt ccagcagcag gcgctttccg tcggacacca
     1141 ccagcggcgg agtttgtctc tccagccgtt tgagcttgtg aatgatccgt ttgcccatgg
     1201 gcggctttga cttcttgtag ccccggctca aaatggccac tttgcgcccc tgctgctgga
     1261 gcgagcgcgc gaaaatttcc acaatgggag ttttcccggt tcctcccacg gtcaggttgc
     1321 cgacgctgac cacctggcat cccagagtgt ggtgccggag aatgcgatgc ttgtaaaggc
     1381 ggagccgaat ctgcaccagc agggcaaaaa cacatgagag cgccttcagc actccgcgga
     1441 ggagagcggc accgcgcccc ttgcggcgtc cttcaat
//
LOCUS       DAOQWR010000251         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_133796_length_1306_cov_4.241407, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000251 DAOQWR010000000
VERSION     DAOQWR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133796_length_1306_cov_4.241407"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="rpoB"
                     /locus_tag="PKK36_12085"
     CDS             <1..530
                     /gene="rpoB"
                     /locus_tag="PKK36_12085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373061.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR95336.1"
                     /translation="HLGLACKMLNMYVETPVFDGIPENKISEMLEKAGLAKDGKTVLY
                     DGRTGEPFDQRVVVGMIYMLKLHHLVTDKIHARAVGPYSLVTQQPLGGKAQYGGQRFG
                     EMEVWAMEAYGAAYALQELLTVKSDDIGGRTRIYESIVKGENTLDAGMPESFNVLMKE
                     MQSLALNIKVNKAGE"
     gene            595..>1306
                     /locus_tag="PKK36_12090"
     CDS             595..>1306
                     /locus_tag="PKK36_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR95337.1"
                     /translation="MFGWETKSRFDTASISLASPETIRSWSFGEVKNPETINYRTFNP
                     EKGGLFCERIFGPTKDWECSCGKFKRIKHKGVICDRCGVEITQARVRRERMGHIELAV
                     PVSHIWFFKCTPSRMGLVLDMTSRSLERVLYYEDYIVIDPGDTPLQEKQLLSEIEFRE
                     AQEKYGETAFEAKMGAEAIRDLMGHIKLAELLQELDAELAQTRSKQTRKKLTKRMKVI
                     DGLLQSGARPEWMILEVLP"
BASE COUNT          316 a          336 c          414 g          240 t
ORIGIN      
        1 cccatctggg cctggcctgc aagatgctta atatgtacgt ggaaactccc gtgtttgacg
       61 gcatccccga gaacaagata tccgagatgc tggagaaagc cggcctggcg aaggacggaa
      121 aaaccgttct ttatgacggg cgcacaggcg agccctttga ccagcgggtg gttgtgggca
      181 tgatctacat gctcaagctt catcacctgg tgaccgacaa gatccatgcg cgcgcagtgg
      241 gaccctattc gcttgtgacg cagcagccgc tgggcgggaa ggcccagtac ggcggacagc
      301 gctttggcga aatggaagtg tgggcgatgg aggcctatgg cgccgcctat gcgctgcagg
      361 aactgctgac cgtcaagagc gacgacatcg gcggccgcac gcggatctat gagtccatcg
      421 tcaagggtga aaacacgctg gacgccggca tgccggaatc gttcaacgtg ctgatgaagg
      481 aaatgcagag ccttgcgctg aacataaaag tgaacaaggc cggtgaataa aatttgaacg
      541 attgtcctcg tccagggcag gaggtgcacg ttggaaagca gcaacgccgc taacatattc
      601 ggttgggaaa ccaaaagcag attcgacacg gcaagcattt cgctggcttc gccggaaaca
      661 atccggtcgt ggagctttgg cgaagtcaag aatccggaaa ccatcaacta ccggacgttc
      721 aatccggaaa agggcggtct gttctgcgag cgtattttcg ggcccacaaa ggactgggag
      781 tgctcctgcg gaaaattcaa gcgcatcaag cacaaaggcg tgatatgcga ccgctgcggc
      841 gtggaaatca cccaggcgcg ggttcgccgc gaacggatgg ggcatattga gctggccgtg
      901 ccggtctcgc acatctggtt tttcaagtgc actcccagcc gcatgggcct tgttctggac
      961 atgaccagcc gcagccttga gcgcgttctt tattatgagg actatatcgt cattgatccg
     1021 ggcgacaccc cgctgcagga aaaacagctg ctgagcgaga tcgaattccg cgaggctcag
     1081 gagaaatacg gcgagacggc gtttgaggcc aagatggggg cggaggccat ccgtgatctg
     1141 atgggccata tcaagcttgc ggagctgctg caggaactgg atgcggaact cgcgcaaacc
     1201 cggagcaagc agacccgaaa gaaactgaca aagcgcatga aggttattga cggcctgctg
     1261 cagagcggcg caaggccgga gtggatgatt ctggaagtgc tgccgg
//
LOCUS       DAOQWR010000252         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_144967_length_1246_cov_3.486986, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000252 DAOQWR010000000
VERSION     DAOQWR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144967_length_1246_cov_3.486986"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PKK36_12095"
     CDS             <1..851
                     /locus_tag="PKK36_12095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR95338.1"
                     /translation="EVIVVDDGSTDAGPEIARSFGARIRLDQQPHQGAPAARNRGVDI
                     AEGELIQFLDADDLLNSAKLERQVPLARDFPEAITCTEYYLQEDQPPSKRHIHLPDAD
                     DPVIFALSAYSLISGPLHWKPSLQRSGGFCVGLSCCQDYDLLLRMACEGWGIHVIHEP
                     LWTLHRVSGSLSSSMLKVLNQLQPVYDAAMHQLSLKSPLSAAHRAAFAGKMAKDARRY
                     LKLGHRQQGLAQLSRARELDAASAVRQAYPGPKSRISFHLLGPVLTEKLAMLSRSHCR
                     KLEKGL"
BASE COUNT          243 a          340 c          355 g          308 t
ORIGIN      
        1 ttgaagtcat tgtggtggat gacggttcaa ccgatgccgg accggagatt gcccgttcct
       61 ttggtgcccg catacgcctg gatcagcagc cccatcaggg tgcgcctgcc gcgcgcaatc
      121 gcggtgtgga catcgcggaa ggagaactga ttcagtttct ggatgcggat gaccttctga
      181 attctgctaa actggagcgg caggttccac tcgcccgcga tttcccggag gccataacgt
      241 gcactgagta ttatttgcag gaggatcagc cgccatccaa acgacacatt catttgccgg
      301 atgccgatga ccccgtgata tttgctctta gtgcctattc gttaatttcg gggccactgc
      361 attggaaacc atccttgcag agatcaggtg gattctgcgt gggtttgtct tgctgtcagg
      421 attatgatct tctgcttcgg atggcttgtg aggggtgggg gattcatgtg attcacgaac
      481 cgctctggac gctgcatcgc gtttctggca gcctctccag ttccatgctc aaggttctca
      541 atcagctgca gccggtttat gatgcagcca tgcatcaatt gtccctcaaa tcccccttgt
      601 ccgccgcgca tcgtgccgcc tttgcgggaa agatggcgaa ggatgcccgc cgttatctta
      661 aactgggcca tcggcagcag ggcttggcac agttaagtcg tgcgcgtgaa ctagatgccg
      721 catctgctgt gcgtcaggcg tatcccggcc cgaagtcacg catcagtttc caccttctgg
      781 gtccggttct gacagagaag cttgcaatgt tgagccggag ccactgccgg aaactggaga
      841 aagggctgtg agcaggcaca tcacatttat tgtctgtcgg gattctctcg gggctgtttt
      901 cgattccatt gccatggctc ccgctgcaaa catgcgccga cggggctgtg aagcagatat
      961 tgtatgcatg gcccccgttg gagaatatgt ccggccggcg cttcgaaagc gctggcagga
     1021 ccgcatgaac accgcagaac agaagctccc gggcggcctg aaccgaattc ccgtcagtcc
     1081 cagccgtttc agcagatggc tggatgattc acagcccctg gctcgatgga tgaggcgaat
     1141 tcccgatgat cgtttgttga tatgctgcgg tgcccctgcg gcatattccg ctctaaaagc
     1201 ccgtcgcaaa actgggagca gtgtccgtct cattttttat tcatta
//
LOCUS       DAOQWR010000253         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_199006_length_1036_cov_2.247706, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000253 DAOQWR010000000
VERSION     DAOQWR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199006_length_1036_cov_2.247706"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKK36_12100"
     CDS             complement(<1..586)
                     /locus_tag="PKK36_12100"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNR95339.1"
                     /translation="MRILKVVSLMLFIAISDCGLCQSSTSFLQMDNPVIRVLISDSTA
                     DALITPTSPFSARTLEGEEILEVPPGLEISISRGDNSVILRDNASSYTTEALCMEPLD
                     TDGSFILQKAGLDEKNPGRSYEGRLEIFPGEGGMLEVIIVLPMEKYLMGVVPYEIGPD
                     SPIEAMCAQAVAARSEAFVALVTRKYAGKHHDICS"
     gene            823..>1036
                     /locus_tag="PKK36_12105"
     CDS             823..>1036
                     /locus_tag="PKK36_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR95340.1"
                     /translation="MSDRKTDILVVGCGIAGATAALTAADSGADVILITSAADLDDGN
                     TSYAQGGIVYRGLDDSAELLARDITAA"
BASE COUNT          276 a          252 c          248 g          260 t
ORIGIN      
        1 cggaacagat gtcatgatgc ttcccggcgt atttccgggt cacaagtgcg acgaatgcct
       61 cggaccgggc tgcaaccgct tgagcacaca tggcctcaat gggtgaatca gggccgattt
      121 cataaggcac cactcccatt aaatatttct ccatgggaag aacaataata acctcaagca
      181 ttccaccttc accggggaaa atctcgaggc gtccttcata tgatctgcct ggattcttct
      241 cgtccaaccc cgccttttgc aatatgaaac tgccatcggt atccagcggt tccatgcaaa
      301 gcgcttctgt ggtatatgaa ctggcgttgt ctctgagaat aacggagttg tcaccccggg
      361 aaatggagat ctccaaacct ggaggcactt ccaatatttc ttcaccctca agggtgcggg
      421 cagagaatgg cgatgtcggg gttatgaggg catctgcagt ggaatcagaa atcagcacac
      481 gaatgacggg attgtccatc tggagaaacg atgtgctgga ctggcacaaa ccacagtccg
      541 atatcgcaat aaacagcatc aaggaaacaa ctttcaatat tctcattagc ctctccccat
      601 aataaaaagc tatttgttcc aattcacaca aatctcacat gtcgtcaaag aatatttgtt
      661 cttgtgattg taataaactg tccggcatac cggacaagtc aaattgtttt tcggactgcg
      721 gcttaaaatc tgtcaggaaa tagattgaca ttgccggagc gttttaaacg ataagggaat
      781 ctgattttga atctcagaaa aaccatgcgg agtttcagta aagtgtctga tcgcaaaacg
      841 gatatactcg ttgttggttg cggcattgcc ggagcaacgg ccgccctcac cgccgcagat
      901 tcgggcgccg atgtgattct gattacaagc gccgccgatc tggatgatgg caatacatcc
      961 tatgcccagg gcggaattgt atatcgcggt cttgatgatt ctgcggaact tcttgcgcgt
     1021 gacattactg cagcgg
//
LOCUS       DAOQWR010000254         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1057
            NODE_211288_length_1001_cov_2.300211, whole genome shotgun
            sequence.
ACCESSION   DAOQWR010000254 DAOQWR010000000
VERSION     DAOQWR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852400
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 14:01:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,422
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,369
            CDSs (with protein)               :: 2,369
            Genes (RNA)                       :: 43
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211288_length_1001_cov_2.300211"
                     /isolate="AMR_MDS_1057"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PKK36_12110"
     CDS             <1..811
                     /locus_tag="PKK36_12110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR95341.1"
                     /translation="IHRFIAVSDEVAELLRQAVPHRAGDILTKACAVEVPAHLNRNYS
                     GSDESLQIVYAGRITNHEKRVYKLLPLAEALRKRGVDFHLRIIGDGGFRWGLNKAVRE
                     LTPSLQKCIVIEGPRSPADMASVWEAADVCVLVSDREGTAVSMLEAMAHGCVPVVTRV
                     SGTAAVIDHGINGFVSDIDDLEAMAGHIARLAADRTVLAECGRKGPEKISADYSLKDY
                     LEWFQSLVDGVWSNPPRKWPVAKPLIMPNAADMRPGLWTRLKRELCAKRDS"
BASE COUNT          203 a          246 c          303 g          249 t
ORIGIN      
        1 gattcaccgg tttatcgcgg tcagcgatga agtggcggaa cttctgcgtc aggctgttcc
       61 gcatcgcgcc ggggatattc tcacaaaagc ctgcgcggtg gaggttcctg cgcatctgaa
      121 ccgaaactat tccgggtccg atgagtcact gcagattgta tatgccgggc gaatcactaa
      181 tcatgaaaag cgggtttata aacttttgcc gcttgccgaa gctttgcgga agcggggcgt
      241 tgattttcac ctgcgcatca tcggggatgg cgggttccgc tggggtctga acaaagccgt
      301 tcgggaactg actcccagtc tgcagaaatg cattgtgatc gagggccccc ggtccccggc
      361 ggatatggcc tctgtgtggg aagcggcgga tgtttgtgtg ctggtttcag atcgggaggg
      421 cacggcggtt tccatgcttg aggccatggc ccatggctgt gttcccgttg tgacccgcgt
      481 cagcggcaca gcggcggtca ttgatcacgg gatcaacggc tttgtctccg acattgatga
      541 tcttgaggcg atggccggtc acattgcaag gctggcagcg gacagaactg tgctggcgga
      601 atgcggacgg aagggtcctg aaaagatttc cgcagactat tcactgaaag attatctgga
      661 atggtttcaa agtctggttg acggggtatg gagtaatccc ccccggaaat ggcccgtcgc
      721 gaaaccgttg attatgccaa acgctgcgga tatgcgtccc ggcctttgga cacgccttaa
      781 gcgggaactt tgcgcgaagc gagattcttg ataagtcaca ttaaactgtc tgtatcattg
      841 gcataggaca tgagtttcaa cagtttcagt tatgcgctgt ttctgccgct cgttctggtg
      901 gtgacgcaac ttctgaggca tcgcataaca gctcgaaatg cattccttct ggcggccagc
      961 tatttcttct acggctcatg ggactggaga ttcctgagcc t
//
